BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0916 (nfi) DE:endonuclease V (nfi) (194 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||B75049 endonuclease v (nfi) PAB0916 - Pyrococcus abyssi (st... 383 e-106 pir||F71111 hypothetical protein PH0661 - Pyrococcus horikoshii ... 280 1e-74 gb|AAK42594.1| Endonuclease V (nfi) [Sulfolobus solfataricus] 108 5e-23 gi|11497746 conserved hypothetical protein [Archaeoglobus fulgid... 100 2e-20 emb|CAC11602.1| (AL445064) endonuclease V related protein [Therm... 98 7e-20 pir||C72747 probable endonuclease V APE0506 - Aeropyrum pernix (... 89 3e-17 gb|AAF33502.1| (AF170176) 95% identity over 218 of 233 amino aci... 87 1e-16 pir||B72202 endonuclease V - Thermotoga maritima (strain MSB8) >... 86 3e-16 pir||A82625 endonuclease V (deoxyinosine 3'endonuclease) XF1892 ... 86 3e-16 pir||A65207 hypothetical 24.9 kD protein in heme-hupa intergenic... 85 5e-16 sp|P32679|NFI_ECOLI ENDONUCLEASE V (DEOXYINOSINE 3'ENDONUCLEASE) 85 5e-16 pir||D70349 conserved hypothetical protein aq_548 - Aquifex aeol... 78 7e-14 pir||T35251 probable endonuclease - Streptomyces coelicolor >gi|... 72 5e-12 >pir||B75049 endonuclease v (nfi) PAB0916 - Pyrococcus abyssi (strain Orsay) >gi|5458800|emb|CAB50287.1| (AJ248287) endonuclease V (nfi) [Pyrococcus abyssi] Length = 194 Score = 383 bits (974), Expect = e-106 Identities = 194/194 (100%), Positives = 194/194 (100%) Query: 1 MLEKIAEVQKKLSKRIVEKEVRMVSKIAAVDVSYKGNKARVALVICSFPDCKVLKTKVLE 60 MLEKIAEVQKKLSKRIVEKEVRMVSKIAAVDVSYKGNKARVALVICSFPDCKVLKTKVLE Sbjct: 1 MLEKIAEVQKKLSKRIVEKEVRMVSKIAAVDVSYKGNKARVALVICSFPDCKVLKTKVLE 60 Query: 61 TEVSFPYIPTFFFLRETRPILLVTKGEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTI 120 TEVSFPYIPTFFFLRETRPILLVTKGEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTI Sbjct: 61 TEVSFPYIPTFFFLRETRPILLVTKGEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTI 120 Query: 121 GVAKKLLRGTPENSYRKVGKAYVSVGNMITLKDAVRIIEKLLDGGYPKPLKLADKLSKGK 180 GVAKKLLRGTPENSYRKVGKAYVSVGNMITLKDAVRIIEKLLDGGYPKPLKLADKLSKGK Sbjct: 121 GVAKKLLRGTPENSYRKVGKAYVSVGNMITLKDAVRIIEKLLDGGYPKPLKLADKLSKGK 180 Query: 181 ISEDENTLPSDKTS 194 ISEDENTLPSDKTS Sbjct: 181 ISEDENTLPSDKTS 194 >pir||F71111 hypothetical protein PH0661 - Pyrococcus horikoshii >gi|3257069|dbj|BAA29752.1| (AP000003) 211aa long hypothetical protein [Pyrococcus horikoshii] Length = 211 Score = 280 bits (708), Expect = 1e-74 Identities = 141/195 (72%), Positives = 167/195 (85%), Gaps = 2/195 (1%) Query: 1 MLEKIAEVQKKLSKRIVEKEVRMVSKIAAVDVSYKGNKARVALVICSFPDCKVLKTKVLE 60 MLE+IA +QKKLSK IVE+++ V K+AAVDVSYK KAR ALVI +FP+ +VLKTKV+E Sbjct: 1 MLERIANIQKKLSKSIVERKINEVRKVAAVDVSYKEEKARAALVITTFPEGEVLKTKVIE 60 Query: 61 TEVSFPYIPTFFFLRETRPILLVTKGEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTI 120 T VSFPYIPTFFFLRET+PIL+ TKGE FDVLIVEGHGKAHPR YGLASHIG++L KPTI Sbjct: 61 TTVSFPYIPTFFFLRETKPILIATKGETFDVLIVEGHGKAHPRGYGLASHIGVVLRKPTI 120 Query: 121 GVAKKLLRGTPENSYRKVGKAYVSVGNMITLKDAVRIIEKLLD-GGYPKPLKLADKLSKG 179 GVAK+LL+ TP+++Y+KVGK YVSVGN+ITL+DA +II +LD GYPKPLKLADKLSKG Sbjct: 121 GVAKRLLKNTPKDTYKKVGKVYVSVGNLITLEDATKIIRAILDESGYPKPLKLADKLSKG 180 Query: 180 KISEDENTLPSDKTS 194 +I E +NT PS S Sbjct: 181 RIYEVKNT-PSPNRS 194 >gb|AAK42594.1| Endonuclease V (nfi) [Sulfolobus solfataricus] Length = 198 Score = 108 bits (267), Expect = 5e-23 Identities = 70/193 (36%), Positives = 106/193 (54%), Gaps = 20/193 (10%) Query: 1 MLEKIAEVQKKLSK--RIVEKEVRMVSKIAAVDVSYKGNKARVALVICSFPDCKVLKTKV 58 +LE + ++Q +SK +I + V KI VD++YKGN ++ + + K Sbjct: 6 LLEFLEKLQFLISKNVKISHYGIENVKKICGVDIAYKGNLG-FSVGVSMDINSGDYNYKS 64 Query: 59 LETEVSFPYIPTFFFLRETRPILLVTKGEEFDVLIVEGHGKAHPRKYGLASHIGLILGKP 118 EV+FPYIP F F+RE ++ +G + +L+V+GHG AHPRK G+A+ IG++L P Sbjct: 65 YVGEVNFPYIPGFLFMREAPLMIKAIEGLDCHLLLVDGHGIAHPRKSGIAAVIGVLLDFP 124 Query: 119 TIGVAKKLLRGTPENSYR-------------KVGKAYVSVGNMITLKDAVRIIEKLLDGG 165 TIGVAK L G N K G+ + S GN + L+D + + ++ G Sbjct: 125 TIGVAKSRLTGDLVNESEITYVYLNGEKVGVKFGRYFYSPGNKVDLQDCIELGKR----G 180 Query: 166 YPKPLKLADKLSK 178 YPK LK+AD L+K Sbjct: 181 YPKVLKIADMLTK 193 >gi|11497746 conserved hypothetical protein [Archaeoglobus fulgidus] >gi|7450237|pir||A69266 conserved hypothetical protein AF0129 - Archaeoglobus fulgidus >gi|2650516|gb|AAB91100.1| (AE001097) conserved hypothetical protein [Archaeoglobus fulgidus] Length = 221 Score = 99.8 bits (245), Expect = 2e-20 Identities = 63/203 (31%), Positives = 111/203 (54%), Gaps = 26/203 (12%) Query: 2 LEKIAEVQKKLSKRIVEKEVRMVSK---IAAVDVSYKGNKARVALVICSFPDCKVLKTKV 58 LE++ +Q+++S+ +V +++ + + + VD ++ ++ V +FP+ +V+ V Sbjct: 6 LEELRRIQEEMSRSVVLEDLIPLEELEYVVGVDQAFISDEVVSCAVKLTFPELEVVDKAV 65 Query: 59 LETEVSFPYIPTFFFLRETRPILLVTKG--EEFDVLIVEGHGKAHPRKYGLASHIGLILG 116 +V+FPYIPTF RE P + KG ++ ++V+G G AHPR+ GLA++I L L Sbjct: 66 RVEKVTFPYIPTFLMFREGEPAVNAVKGLVDDRAAIMVDGSGIAHPRRCGLATYIALKLR 125 Query: 117 KPTIGVAKK---------------LLRGTPENSY-----RKVGKAYVSVGNMITLKDAVR 156 KPT+G+ KK LL G+ Y R+ ++S G+ I+ A+ Sbjct: 126 KPTVGITKKRLFGEMVEVEDGLWRLLDGSETIGYALKSCRRCKPIFISPGSYISPDSALE 185 Query: 157 IIEKLLDG-GYPKPLKLADKLSK 178 + K L G P+P+++ADKL+K Sbjct: 186 LTRKCLKGYKLPEPIRIADKLTK 208 >emb|CAC11602.1| (AL445064) endonuclease V related protein [Thermoplasma acidophilum] Length = 323 Score = 97.9 bits (240), Expect = 7e-20 Identities = 66/198 (33%), Positives = 105/198 (52%), Gaps = 21/198 (10%) Query: 2 LEKIAEVQKKLSKRIVEKEVRMVSKIAAVDVSYKGNKARVALVICSFPDCKVLKTKVLET 61 L K+ + Q+ ++++ E +AA DVSY+ +A ++VI D ++ V+ Sbjct: 121 LRKMQQYQEYIARKYSESGAGEFRTVAAFDVSYEERRAFASMVI---QDGSTIEAYVISD 177 Query: 62 EVSFPYIPTFFFLRETRPILLVTKGEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTIG 121 ++FPYIP + RE R I + + + D+L+++G+G HPR GLA+H G++L + +IG Sbjct: 178 GINFPYIPGYLAFREFRFIKKLYR--KADLLLIDGNGILHPRFAGLATHAGVLLDRASIG 235 Query: 122 VAKKLLRGTPENSY-----RKVG-----KAYVSVGNMITLKDAVRIIEKLLDGGYPKPLK 171 VAK L R T R VG VS GN I + + + K+ + YP PLK Sbjct: 236 VAKHLTRSTRHQDIIYMQDRPVGVMIRKNMIVSPGNFIDVNGSADTVRKMWNDRYPWPLK 295 Query: 172 LADKLSK------GKISE 183 LA +LS G++SE Sbjct: 296 LAHQLSTRHAETGGRVSE 313 >pir||C72747 probable endonuclease V APE0506 - Aeropyrum pernix (strain K1) >gi|5104155|dbj|BAA79471.1| (AP000059) 218aa long hypothetical endonuclease V [Aeropyrum pernix] Length = 218 Score = 89.3 bits (218), Expect = 3e-17 Identities = 62/172 (36%), Positives = 88/172 (51%), Gaps = 23/172 (13%) Query: 30 VDVSY--KGNKARVALVICSFPDCKVLKTKVLETEVSFPYIPTFFFLRETRPIL--LVTK 85 +D SY K A V+ S + + +V + V PYIP RE + Sbjct: 4 LDASYSAKDGVGVGAAVLISLETLEPVDCRVYISRVCIPYIPGLLAFRELAVMAPAAAAL 63 Query: 86 GEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTIGVAKKLLRGT----PENSY------ 135 E DV++V+GHG AHPR++G+ASH+G+IL +P+IGVAKK L GT P Y Sbjct: 64 SAEADVVMVDGHGIAHPRRFGIASHVGVILERPSIGVAKKKLVGTLVEGPGGMYVVQDGE 123 Query: 136 -------RKVGKAYVSVGNMITLKDAVRIIEKLL--DGGYPKPLKLADKLSK 178 + + YVS G+ ITL++A I + G P+P +LAD +SK Sbjct: 124 RLAIVLGTRPREVYVSPGHRITLEEAASIARATIRPGGWMPEPTRLADVISK 175 >gb|AAF33502.1| (AF170176) 95% identity over 218 of 233 amino acids with E. coli endonuclease V (NFI) (SW:P32679) [Salmonella typhimurium LT2] Length = 223 Score = 87.0 bits (212), Expect = 1e-16 Identities = 53/182 (29%), Positives = 91/182 (49%), Gaps = 28/182 (15%) Query: 27 IAAVDVSYK--GNKARVALVICSFPDCKVLKTKVLETEVSFPYIPTFFFLRETRPILLVT 84 I DV ++ G R A+V+ +P ++++ KV + PYIP F RE +L Sbjct: 31 IGGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAW 90 Query: 85 K--GEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTIGVAKKLLRGTPE---------- 132 + ++ D+L V+GHG +HPR+ G+ASH GL++ PTIGVAKK L G E Sbjct: 91 EQLSQKPDLLFVDGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSAEPGALS 150 Query: 133 -------------NSYRKVGKAYVSVGNMITLKDAVRIIEKLLDG-GYPKPLKLADKLSK 178 S + +++ G+ ++ A+ +++ + G P+P + AD ++ Sbjct: 151 PLMDKGEQLAWVWRSKARCNPLFIATGHRVSTDSALAWVQRCMKGYRLPEPTRWADAVAS 210 Query: 179 GK 180 G+ Sbjct: 211 GR 212 >pir||B72202 endonuclease V - Thermotoga maritima (strain MSB8) >gi|4982450|gb|AAD36927.1|AE001823_9 (AE001823) endonuclease V [Thermotoga maritima] Length = 225 Score = 85.8 bits (209), Expect = 3e-16 Identities = 76/216 (35%), Positives = 110/216 (50%), Gaps = 45/216 (20%) Query: 3 EKIAEVQKKLSKRI----VEKEVRMVSKIAAVDVSYKGNKARVA-LVICSFPDCKVLKTK 57 E+ +VQ +L K+I E E V A VD+S+ G + +A +V+ +P K+L+ Sbjct: 14 EEAIKVQNELRKKIKLTPYEGEPEYV---AGVDLSFPGKEEGLAVIVVLEYPSFKILEVV 70 Query: 58 VLETEVSFPYIPTFFFLRETRPIL-----LVTKGEEFDVLIVEGHGKAHPRKYGLASHIG 112 E++FPYIP RE L L TK DV++ +G G AHPRK G+ASH+G Sbjct: 71 SERGEITFPYIPGLLAFREGPLFLKAWEKLRTKP---DVVVFDGQGLAHPRKLGIASHMG 127 Query: 113 LILGKPTIGVAKKLLRGT---PEN-----SY-------------RKVGKA--YVSVGNMI 149 L + PTIGVAK L GT PE+ SY K G A +VS G+++ Sbjct: 128 LFIEIPTIGVAKSRLYGTFKMPEDKRCSWSYLYDGEEIIGCVIRTKEGSAPIFVSPGHLM 187 Query: 150 TLKDAVRIIEKLLDGG--YPKPLKLA----DKLSKG 179 ++ + R+I+ G P+P +LA +L KG Sbjct: 188 DVESSKRLIKAFTLPGRRIPEPTRLAHIYTQRLKKG 223 >pir||A82625 endonuclease V (deoxyinosine 3'endonuclease) XF1892 [imported] - Xylella fastidiosa (strain 9a5c) >gi|9106985|gb|AAF84698.1|AE004009_5 (AE004009) endonuclease V (deoxyinosine 3'endonuclease) [Xylella fastidiosa] Length = 254 Score = 85.8 bits (209), Expect = 3e-16 Identities = 68/215 (31%), Positives = 112/215 (51%), Gaps = 32/215 (14%) Query: 8 VQKKLSKRIVEKE-VRMVSK---IAAVDVSYK--GNKARVALVICSFPDCKVLKTKVLET 61 +Q +++RIV K+ + ++S+ +A DV ++ G R A V+ + D K+L+T V+ Sbjct: 38 LQSDMAERIVLKDDLNLLSEPTLLAGFDVGFEDEGRTTRAAAVLMNACDLKLLETHVVRV 97 Query: 62 EVSFPYIPTFFFLRETRPIL-LVTKGEEFDVLI-VEGHGKAHPRKYGLASHIGLILGKPT 119 S PY+P RE +L +T+ L+ V+GHG AHPR+ G+A+H GL+ P Sbjct: 98 PTSMPYVPGLLSFRELPALLQALTQLSRIPALVFVDGHGIAHPRRLGIAAHFGLVTNLPC 157 Query: 120 IGVAKKLLRG---TPENSY----------RKVGKA----------YVSVGNMITLKDAVR 156 IGVAKK L G P ++ +VG A +S G+ I+L A+ Sbjct: 158 IGVAKKRLVGDFVEPGTAFGEHTPILLHGTQVGWALRSKIRCKPLMISPGHKISLHSALT 217 Query: 157 IIEKLLDG-GYPKPLKLADKLSKGKISEDENTLPS 190 ++ L+G P+P + AD+L+ + + + LPS Sbjct: 218 WTQRCLNGYRLPEPTRQADRLASRRGQKIVSDLPS 252 >pir||A65207 hypothetical 24.9 kD protein in heme-hupa intergenic region - Escherichia coli (strain K-12) >gi|409792|gb|AAC43096.1| (U00006) similar to O.berteriana mitochon. protein involved in cytochrome c biogenesis [Escherichia coli] >gi|1790431|gb|AAC76972.1| (AE000473) endonuclease V (deoxyinosine 3'endoduclease) [Escherichia coli K12] Length = 225 Score = 85.0 bits (207), Expect = 5e-16 Identities = 52/179 (29%), Positives = 91/179 (50%), Gaps = 28/179 (15%) Query: 27 IAAVDVSYK--GNKARVALVICSFPDCKVLKTKVLETEVSFPYIPTFFFLRETRPILLVT 84 IA DV ++ G R A+V+ +P ++++ KV + PYIP F RE +L Sbjct: 33 IAGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAW 92 Query: 85 K--GEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTIGVAKKLLRGTPE---------- 132 + ++ D++ V+GHG +HPR+ G+ASH GL++ PTIGVAKK L G E Sbjct: 93 EMLSQKPDLVFVDGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALA 152 Query: 133 -------------NSYRKVGKAYVSVGNMITLKDAVRIIEKLLDG-GYPKPLKLADKLS 177 S + +++ G+ +++ A+ +++ + G P+P + AD ++ Sbjct: 153 PLMDKGEQLAWVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVA 211 >sp|P32679|NFI_ECOLI ENDONUCLEASE V (DEOXYINOSINE 3'ENDONUCLEASE) Length = 223 Score = 85.0 bits (207), Expect = 5e-16 Identities = 52/179 (29%), Positives = 91/179 (50%), Gaps = 28/179 (15%) Query: 27 IAAVDVSYK--GNKARVALVICSFPDCKVLKTKVLETEVSFPYIPTFFFLRETRPILLVT 84 IA DV ++ G R A+V+ +P ++++ KV + PYIP F RE +L Sbjct: 31 IAGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAW 90 Query: 85 K--GEEFDVLIVEGHGKAHPRKYGLASHIGLILGKPTIGVAKKLLRGTPE---------- 132 + ++ D++ V+GHG +HPR+ G+ASH GL++ PTIGVAKK L G E Sbjct: 91 EMLSQKPDLVFVDGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALA 150 Query: 133 -------------NSYRKVGKAYVSVGNMITLKDAVRIIEKLLDG-GYPKPLKLADKLS 177 S + +++ G+ +++ A+ +++ + G P+P + AD ++ Sbjct: 151 PLMDKGEQLAWVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVA 209 >pir||D70349 conserved hypothetical protein aq_548 - Aquifex aeolicus >gi|2983195|gb|AAC06795.1| (AE000695) hypothetical protein [Aquifex aeolicus] Length = 188 Score = 78.0 bits (189), Expect = 7e-14 Identities = 57/165 (34%), Positives = 80/165 (47%), Gaps = 27/165 (16%) Query: 38 KARVALVICSFPDCKVLKTKVLETEVSFPYIPTFFFLRETRPILLV---TKGEEFDVLIV 94 +A +LV+ K + V++ V FPYIPTF RE P+LL T + DV + Sbjct: 14 RAWASLVVVELKTLKPVYQHVVKDIVDFPYIPTFLAFREM-PLLLKLYETAKVKPDVYFI 72 Query: 95 EGHGKAHPRKYGLASHIGLILGKPTIGVAKKLLRGTPEN--------SYRK--------- 137 +G G AHPR G+ASH G+ G+ T+GVAK L G + +Y K Sbjct: 73 DGQGIAHPRGCGIASHFGVETGEVTVGVAKSKLFGYAKEPAPQRGSYTYLKYKGKIIGAV 132 Query: 138 ------VGKAYVSVGNMITLKDAVRIIEKLLDGGYPKPLKLADKL 176 YVSVG+ I+LK A+ ++ K P+P +LA L Sbjct: 133 VRTKDNTAPVYVSVGHRISLKTAIDLVLKTSKYRVPEPTRLAHNL 177 >pir||T35251 probable endonuclease - Streptomyces coelicolor >gi|4584473|emb|CAB40676.1| (AL049587) putative endonuclease [Streptomyces coelicolor A3(2)] Length = 233 Score = 71.8 bits (173), Expect = 5e-12 Identities = 50/167 (29%), Positives = 77/167 (45%), Gaps = 13/167 (7%) Query: 3 EKIAEVQKKLSKRIVEKEVRM---VSKIAAVDVSYKGNKARVAL--VICSFPDCKVLKTK 57 E+ VQ +L R+V E ++ VDV+Y + VA V+ V+ Sbjct: 17 ERARAVQDELRARVVLDEPGPPPGTGRVTGVDVAYDDERDVVAAAAVVLDAGTLAVVAEA 76 Query: 58 VLETEVSFPYIPTFFFLRETRPILLVTKGEEFD--VLIVEGHGKAHPRKYGLASHIGLIL 115 +SFPY+P RE +L + +++ +G+G AHPR++GLASH+G++ Sbjct: 77 TAVGRISFPYVPGLLAFREIPTVLAALEALPCPPGLVVCDGYGLAHPRRFGLASHLGVLT 136 Query: 116 GKPTIGVAKKLLR------GTPENSYRKVGKAYVSVGNMITLKDAVR 156 G PTIGVAK TP S + VG + +D V+ Sbjct: 137 GLPTIGVAKNPFTFTHDDPDTPRGSTSPLLAGAEEVGRAVRTRDGVK 183 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.317 0.138 0.381 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 70129166 Number of Sequences: 2977 Number of extensions: 2890777 Number of successful extensions: 5880 Number of sequences better than 1.0e-10: 13 Number of HSP's better than 0.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 5858 Number of HSP's gapped (non-prelim): 13 length of query: 194 length of database: 189,106,746 effective HSP length: 57 effective length of query: 137 effective length of database: 154,992,987 effective search space: 21234039219 effective search space used: 21234039219 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.6 bits) S2: 163 (67.9 bits)