BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB0943 (nifs-like) DE:CAPREOMYCIN ACETYLTRANSFERASE or NIFS-LIKE PROTEIN (386 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75054 capreomycin acetyltransferase or nifs-like protein P... 771 0.0 pir||A71107 hypothetical protein PH0626 - Pyrococcus horikoshii ... 610 e-174 gb|AAK42385.1| NifS protein homolog (nifS) [Sulfolobus solfatari... 136 3e-31 pir||H72558 hypothetical protein APE1754 - Aeropyrum pernix (str... 113 4e-24 sp|P42253|YCBU_BACSU HYPOTHETICAL 40.8 KD PROTEIN IN PCP-LMRB IN... 111 1e-23 pir||E75213 nifs protein PAB0157 - Pyrococcus abyssi (strain Ors... 93 4e-18 pir||G69051 nifS protein - Methanobacterium thermoautotrophicum ... 90 4e-17 pir||JC4557 capreomycin acetyltransferase (EC 2.3.1.-) - Strepto... 82 1e-14 gb|AAA92036.1| (U13077) capreomycin acetyltransferase [Streptomy... 81 3e-14 pir||F70019 nifS protein homolog yurW - Bacillus subtilis >gi|26... 81 3e-14 pir||A83275 hypothetical protein PA2958 [imported] - Pseudomonas... 76 8e-13 pir||A72506 probable tRNA splicing protein SPL1 APE2023 - Aeropy... 75 2e-12 gi|11499012 aspartate aminotransferase (aspC) [Archaeoglobus ful... 73 6e-12 pir||S77167 L-cysteine desulfhydrase (EC 4.4.1.-) - Synechocysti... 71 3e-11 gb|AAD02935.1| (AF061964) L-cysteine/cystine lyase C-DES [Synech... 70 4e-11 pir||G72047 nifs-related aminotransferase - Chlamydophila pneumo... 69 8e-11 pdb|1ELU|A Chain A, Complex Between The Cystine C-S Lyase C-Des ... 69 8e-11 >pir||E75054 capreomycin acetyltransferase or nifs-like protein PAB0943 - Pyrococcus abyssi (strain Orsay) >gi|5458843|emb|CAB50330.1| (AJ248287) CAPREOMYCIN ACETYLTRANSFERASE or NIFS-LIKE PROTEIN [Pyrococcus abyssi] Length = 386 Score = 771 bits (1970), Expect = 0.0 Identities = 386/386 (100%), Positives = 386/386 (100%) Query: 1 MRELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVL 60 MRELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVL Sbjct: 1 MRELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVL 60 Query: 61 SEAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKH 120 SEAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKH Sbjct: 61 SEAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKH 120 Query: 121 NLKVEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVI 180 NLKVEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVI Sbjct: 121 NLKVEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVI 180 Query: 181 VDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTE 240 VDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTE Sbjct: 181 VDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTE 240 Query: 241 PPVPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKE 300 PPVPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKE Sbjct: 241 PPVPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKE 300 Query: 301 DLKLAEMVREWALEKGFNVLGDSQITLIVTGIEFEREKEIVRKLNEMGIIVSQRGAKGIH 360 DLKLAEMVREWALEKGFNVLGDSQITLIVTGIEFEREKEIVRKLNEMGIIVSQRGAKGIH Sbjct: 301 DLKLAEMVREWALEKGFNVLGDSQITLIVTGIEFEREKEIVRKLNEMGIIVSQRGAKGIH 360 Query: 361 GIRVSPHLYNTKEDIEKFIEGIERFL 386 GIRVSPHLYNTKEDIEKFIEGIERFL Sbjct: 361 GIRVSPHLYNTKEDIEKFIEGIERFL 386 >pir||A71107 hypothetical protein PH0626 - Pyrococcus horikoshii >gi|3257032|dbj|BAA29715.1| (AP000003) 385aa long hypothetical protein [Pyrococcus horikoshii] Length = 385 Score = 610 bits (1557), Expect = e-174 Identities = 288/386 (74%), Positives = 343/386 (88%), Gaps = 1/386 (0%) Query: 1 MRELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVL 60 MR+LFP L++F AYLNTAGLGLLP+ VL+++ EF L ++ YK+GINAVE++D +YLEP L Sbjct: 1 MRDLFPALKKFNAYLNTAGLGLLPLNVLEDIAEFFLSMLAYKDGINAVEVMDTIYLEPTL 60 Query: 61 SEAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKH 120 EAAKLM+T RN+ LS+QTTDGLKRALLAL+PK+ M I+SFDLEFPTISA+V SY++ Sbjct: 61 KEAAKLMKTSHRNITLSLQTTDGLKRALLALKPKRGMNIVSFDLEFPTISAIVNSYSKLT 120 Query: 121 NLKVEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVI 180 +KV+VI++ G+YD+SDVEKA+DD TFAVVFSDVQWITGQ+MPTKEI+EIAHEHGAWVI Sbjct: 121 GIKVKVIKNNGGIYDLSDVEKAVDDNTFAVVFSDVQWITGQRMPTKEIAEIAHEHGAWVI 180 Query: 181 VDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTE 240 VDAVQSLGAL+VYPEK+GVD+LVAGGEKWLLNPNMGSG+M+LS+RF+EE+KP++GLLNTE Sbjct: 181 VDAVQSLGALEVYPEKMGVDILVAGGEKWLLNPNMGSGVMFLSNRFIEESKPVLGLLNTE 240 Query: 241 PPVPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKE 300 PPVPWS+WWGDKDKDLW++LPVR DARKLDPGTP YL+AVALK SLELINDVGIKKIE+ Sbjct: 241 PPVPWSEWWGDKDKDLWEMLPVRKDARKLDPGTPPYLAAVALKASLELINDVGIKKIERN 300 Query: 301 DLKLAEMVREWALEKGFNVLGDSQITLIVTGIEFEREKEIVRKLNEMGIIVSQRGAKGIH 360 +L+LAE V+EWA EKGF LG SQI LI+ G++FE EK+IV KL E IIVSQRGAKGIH Sbjct: 301 NLRLAERVKEWANEKGFKTLGSSQIVLIM-GLDFEEEKKIVNKLKEDKIIVSQRGAKGIH 359 Query: 361 GIRVSPHLYNTKEDIEKFIEGIERFL 386 GIRVSPH YN K+DIEKF EG+E L Sbjct: 360 GIRVSPHFYNNKDDIEKFAEGMESLL 385 >gb|AAK42385.1| NifS protein homolog (nifS) [Sulfolobus solfataricus] Length = 376 Score = 136 bits (340), Expect = 3e-31 Identities = 107/385 (27%), Positives = 187/385 (47%), Gaps = 30/385 (7%) Query: 2 RELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVLS 61 RE P R++ YLN A + P+ L E +L +V G AV + L + S Sbjct: 10 RENVPVTRKY-VYLNHASVSPTPLPSLFEAYRYLYEVAN--RGSIAVNEEEEDELYHIRS 66 Query: 62 EAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKHN 121 + + L+ S + L T+ G+ LE K + I++ +LEFPT+ +K Sbjct: 67 KISNLVGAYSDEISLIPNTSYGVNLVAHGLEWKGDDNIVTDNLEFPTVVYPFLKLTKK-G 125 Query: 122 LKVEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIV 181 +K+ ++E+ ++ D+ ID T V S V + TG ++ ++I + A E+ V++ Sbjct: 126 VKINIVETNPYTFE-EDIISHIDKNTRLVAISHVSFNTGLKVDVRKIVKAARENNTLVLL 184 Query: 182 DAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEP 241 D +QS GA+++ ++LG+D +AGG KWL++P GSG +Y+ +E+ Sbjct: 185 DIIQSAGAVKINVKELGIDFAIAGGYKWLMSP-QGSGFIYVKRGLIEDP----------- 232 Query: 242 PVPWSDWWGDKDKDLWDILP----VRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKI 297 P+ W D D P + R+ + GT + ++L S E+I + ++ I Sbjct: 233 --PFYGW--KTSADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLAKSCEIIGE-NMELI 287 Query: 298 EKEDLKLAEMVREWALEKGFNVLGDSQITLIVTGIEFEREKEIVRKLNEMGIIVSQRGAK 357 E L++ A + V+ + ++ ++ KEI ++L + I+VS RG Sbjct: 288 ESSVTNLSQFAIRLAKDHSMEVITPEDKRAGIVIVKVKKPKEIAKELLKENIVVSPRG-- 345 Query: 358 GIHGIRVSPHLYNTKEDIEKFIEGI 382 GIR+S H YNT+E+++K IE I Sbjct: 346 --EGIRISTHFYNTEEEVQKTIEKI 368 >pir||H72558 hypothetical protein APE1754 - Aeropyrum pernix (strain K1) >gi|5105444|dbj|BAA80757.1| (AP000062) 415aa long hypothetical protein [Aeropyrum pernix] Length = 415 Score = 113 bits (280), Expect = 4e-24 Identities = 102/391 (26%), Positives = 175/391 (44%), Gaps = 16/391 (4%) Query: 1 MRELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVL 60 +RE P R + YL+TAG G L + V V FL + EG E LD + L Sbjct: 33 VRERIPIFSR-KVYLDTAGAGPLTLDVKDAVEGFL--ELWNSEGEPWGEALDDILESRRL 89 Query: 61 SEAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKH 120 A+++ + V T GL L +L+ + ++ + FPT + A + Sbjct: 90 F--AQMVGAKVEEVAAVPGVTYGLNAFLSSLKLPPDSNVVVSETNFPTGVYTFHAMARRG 147 Query: 121 NLK-VEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWV 179 ++ V + + +G + E+ IDD T V V WITG + + ++E+AH HGA + Sbjct: 148 LIREVRIASASDGCVGLDGFERLIDDSTSIVYVDMVSWITGCREDLRTLAELAHSHGALM 207 Query: 180 IVDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPII-GLLN 238 + D + G + +KLG+D G KWLL + G G Y+S+ L +P+ G + Sbjct: 208 VSDVFHAAGVYPIDVKKLGLDAAFTGSYKWLLGLH-GGGFAYVSEELLPSLEPMFSGWMA 266 Query: 239 TEPPVPWSDWWGDK-DKDLWDILPVR--GDARKLDPGTPAYLSAVALKPSLELINDVGIK 295 + V GD +++ R DA+ L+ GT ++ V+LK SL++++ Sbjct: 267 IDDSVMERMARGDPLFSRPFNVREFRRASDAKVLEWGTWPAIAFVSLKASLKMLSAYDAP 326 Query: 296 KIEKEDL-KLAEMVREWALEKGFNVLGDSQITLIVTGIEFEREKEIVRKLNEMGIIVSQR 354 + +L + + + + G + + + + + R L GI+ S R Sbjct: 327 GLYASHTGRLVKRLIDSLTDMGVETVTPRGMAAGIVVFRHRHPERLGRFLESRGIVASVR 386 Query: 355 GAKGIHGIRVSPHLYNTKEDIEKFIEGIERF 385 +RVS H YN++ED+E + +E F Sbjct: 387 PG----SVRVSIHFYNSREDVEALLRALEEF 413 >sp|P42253|YCBU_BACSU HYPOTHETICAL 40.8 KD PROTEIN IN PCP-LMRB INTERGENIC REGION PRECURSOR (ORF21) >gi|7475675|pir||E69754 nifS protein homolog ycbU - Bacillus subtilis >gi|2415717|dbj|BAA22227.1| (AB000617) YcbU [Bacillus subtilis] >gi|2632552|emb|CAB12060.1| (Z99105) similar to NifS protein homolog [Bacillus subtilis] Length = 370 Score = 111 bits (275), Expect = 1e-23 Identities = 103/382 (26%), Positives = 167/382 (42%), Gaps = 28/382 (7%) Query: 2 RELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVLS 61 R+LFP L+ L + L V + + ++ D + YK G N E ++ E + Sbjct: 9 RQLFPTLQT-HTMLASCSQSALAEPVSRAIQDYY-DSLLYK-GTNWKEAIEKT--EFARN 63 Query: 62 EAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKHN 121 E AKL+ V + +D L L ++ D++FP + V +++++ Sbjct: 64 EFAKLIGAEPDEVAIVPSVSDALVSVASYLTAFGKKHVVYTDMDFPAVPHVWQAHSD--- 120 Query: 122 LKVEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIV 181 V VI S +G+ + E I DET V + G K I+EI+ G+ + V Sbjct: 121 YTVSVIPSIDGVLPLEQYETHISDETVLTCVPHVHYRDGYVQDIKAIAEISQRKGSLLFV 180 Query: 182 DAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEP 241 DA QS G + + ++ GVD+L AG K+LL G +Y+ + KP Sbjct: 181 DAYQSAGHIPIDVKEWGVDMLAAGTRKYLLG-IPGVAFLYVRKELADALKP--------- 230 Query: 242 PVPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKED 301 S W+G + V AR+ GTPA++S A +L L+N +G+ I Sbjct: 231 --KASAWFGRESGFDGAYAKV---ARRFQTGTPAFISVYAAAAALSLLNHIGVSHIRDHV 285 Query: 302 LKLAEMVREWALEKGFNV-LGDSQITLIVTGIEFEREKEIVRKLNEMGIIVSQRGAKGIH 360 + ++A EKG + I + I ER E L + +I + R + Sbjct: 286 KTICADAVQYAAEKGLQLAAAQGGIQPGMVAIRDERASETAGLLKKKKVICAPRE----N 341 Query: 361 GIRVSPHLYNTKEDIEKFIEGI 382 IR++PH YNTKE++ I+ I Sbjct: 342 VIRLAPHFYNTKEEMRHAIDEI 363 >pir||E75213 nifs protein PAB0157 - Pyrococcus abyssi (strain Orsay) >gi|5457665|emb|CAB49156.1| (AJ248283) NIFS PROTEIN [Pyrococcus abyssi] Length = 401 Score = 93.2 bits (228), Expect = 4e-18 Identities = 95/392 (24%), Positives = 162/392 (41%), Gaps = 35/392 (8%) Query: 14 YLNTAGLGLLPITVLKEVTEFLLDV-IKYKEGINAVEILDPMYLEPVLSEAAKLMRTRSR 72 Y + L P V++ + E+ L G++ + + E A + Sbjct: 19 YFDNTATSLTPKPVIEAMDEYYLRYRANVHRGVHRLSQMATQKYEESRKVVADFINAEFD 78 Query: 73 NVGLSIQTTDGLKRALLALEP--KKNMKIISFDLEFPTISAVVKSYAEKHNLKVEVIESK 130 + + T++ L L LE KK KI++ E + + A+K LK+E IE Sbjct: 79 EIAFTKNTSESLNLVALGLEHLFKKGDKIVTTPYEHHSNLLPWQRLAKKKGLKLEFIEGD 138 Query: 131 N-GLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVDAVQSLGA 189 + G D++D EK I V V G +E+ ++ E GA +VDA QS+G Sbjct: 139 DEGNLDLADAEKKIKGAKLVAV-QHVSNALGVIHEVEELGKMVKEEGAIFVVDAAQSVGH 197 Query: 190 LQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPPVPWSDWW 249 ++V +KL D L G K + P G G++Y++ F + EPP+ Sbjct: 198 MEVDVKKLKADFLAFSGHKGPMGPT-GIGVLYINKEFFDV---------FEPPLIGGGTI 247 Query: 250 GDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKEDLKLAEMVR 309 D + + + + + GTP A+ L ++ I +GI KIEK++ KL + Sbjct: 248 EDVELCCYKLTE---PPERFEAGTPNIGGAIGLAAGIKYIEKIGIDKIEKQERKLVKRTT 304 Query: 310 EWALEKGFNVLGDSQITLIVTGIEF--------------EREKEIVRKLNEMGIIVSQRG 355 E E G + + F + K +VR + + V +R Sbjct: 305 EGLDELEIPWYGPRNLDKHAGVVSFNVPPLHPHDVASVLDEHKIMVRSGHHCALPVMKR- 363 Query: 356 AKGIHG-IRVSPHLYNTKEDIEKFIEGIERFL 386 I+G +R S H+YN+ E++E F+ +E + Sbjct: 364 -LKINGTVRASFHVYNSLEEVETFLGVLEELV 394 >pir||G69051 nifS protein - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622499|gb|AAB85866.1| (AE000901) nifS protein [Methanobacterium thermoautotrophicum] Length = 400 Score = 90.1 bits (220), Expect = 4e-17 Identities = 98/405 (24%), Positives = 167/405 (41%), Gaps = 54/405 (13%) Query: 8 LRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVLSEAAKLM 67 L R YL+ A P+ V++ +TE+ + G A + VL KL Sbjct: 15 LLRDHVYLDAASTTPTPLPVVRAMTEYFKEY-NANTGRGAYSL--------VLRATRKLQ 65 Query: 68 RTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKHN------ 121 R + G ++D + + + + I++ L F +VV E H+ Sbjct: 66 EAREKVAGFINASSDEI---VFTKNTSEAINIVAGGLRFRKGDSVVVPNIEHHSNFLPWL 122 Query: 122 ------LKVEVIES-KNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHE 174 + V V+++ + G+ D +E A+D+ T V + + G KEI IAH+ Sbjct: 123 RLRERGVDVRVVKADEGGVVDPGRIEDAVDETTRLVTVTHISNALGTVQDVKEIGRIAHD 182 Query: 175 HGAWVIVDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPII 234 GA +VDA QS+G ++V +G D G K L P +G+G +Y S E +P++ Sbjct: 183 VGALYLVDAAQSIGHMEVDVRAIGADFAAFPGHKGTLGP-VGTGFLYCSTDVQGELEPLM 241 Query: 235 GLLNTEPPVPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGI 294 T V +D + + P K + GT + L S++ +N +GI Sbjct: 242 LGGGTVLDV-------SEDGYVLEDFPA-----KFEAGTLNIAGFIGLGASIDYMNRIGI 289 Query: 295 KKIEKEDLKLAEMVREWALE-KGFNVLGDSQ--ITLIVTGIEFEREKEIVRKLNEMGIIV 351 ++I+K +K+ E + G GD Q ++ I ++ + L+E I Sbjct: 290 RRIQKHGMKMTEELHATVSSIDGIECYGDPQNIYGILSFNINNMDPHDVAKILDETAGIC 349 Query: 352 SQRGAK---------GIH----GIRVSPHLYNTKEDIEKFIEGIE 383 + G +H +R S H YNT E+I+ E +E Sbjct: 350 VRSGHHCAIPAMKHLALHETGGTVRASIHYYNTSEEIQLLGETLE 394 >pir||JC4557 capreomycin acetyltransferase (EC 2.3.1.-) - Streptomyces capreolus >gi|1223838|gb|AAA92035.1| (U13077) capreomycin acetyltransferase [Streptomyces capreolus] >gi|1586532|prf||2204233A capreomycin acetyltransferase [Streptomyces capreolus] Length = 359 Score = 81.9 bits (199), Expect = 1e-14 Identities = 82/333 (24%), Positives = 146/333 (43%), Gaps = 31/333 (9%) Query: 64 AKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKHNLK 123 A LM V + T A A+ +++ ++E+ + + A + + Sbjct: 36 ASLMGAHPDEVAFTDSATRSWLAAFDAVPLGPGDRVLVTEVEYGGNAIPILHRAREVGFE 95 Query: 124 VEVIESKN-GLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVD 182 V I S G D+ + + +D+ V G P +E++ AHE GA V++D Sbjct: 96 VVTIPSDAVGQVDVEALRELVDERVKLVSLVHAPTNGGLVNPVREVASAAHEVGALVLLD 155 Query: 183 AVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPP 242 A QSLG L V ++LGVD++ A G KWL P G+G++ + + +P + L++ Sbjct: 156 ACQSLGQLPVRADELGVDIISATGRKWLRGPR-GTGVLVVCRSAADRLRPRLVDLHS--- 211 Query: 243 VPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKEDL 302 +W G ++ + +R DAR + + + L + D+GI I E Sbjct: 212 ---GEWVGPEE------VKLREDARVYELWETSVADRLGLIAAARYALDLGIDAIAAEVR 262 Query: 303 KLAEMVREWALE-KGFNVLG-DSQITLIVTGIEFEREKEIVR-------------KLNEM 347 AE +R E G V ++++ IVT R+ VR L Sbjct: 263 SRAERLRAGLREVPGVEVRDIGARLSGIVTFTVEGRDPVAVRDELARHDVVVTTSHLTST 322 Query: 348 GIIVSQRGAKGIHGIRVSPHLYNTKEDIEKFIE 380 I +++RG +G+ +R+SPH + + E +++ +E Sbjct: 323 RIDMTRRGVEGL--VRMSPHYFVSPEQVDRAVE 353 >gb|AAA92036.1| (U13077) capreomycin acetyltransferase [Streptomyces capreolus] Length = 289 Score = 80.8 bits (196), Expect = 3e-14 Identities = 70/264 (26%), Positives = 122/264 (45%), Gaps = 30/264 (11%) Query: 132 GLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVDAVQSLGALQ 191 G D+ + + +D+ V G P +E++ AHE GA V++DA QSLG L Sbjct: 35 GQVDVEALRELVDERVKLVSLVHAPTNGGLVNPVREVASAAHEVGALVLLDACQSLGQLP 94 Query: 192 VYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPPVPWSDWWGD 251 V ++LGVD++ A G KWL P G+G++ + + +P + L++ +W G Sbjct: 95 VRADELGVDIISATGRKWLRGPR-GTGVLVVCRSAADRLRPRLVDLHS------GEWVGP 147 Query: 252 KDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKEDLKLAEMVREW 311 ++ + +R DAR + + + L + D+GI I E AE +R Sbjct: 148 EE------VKLREDARVYELWETSVADRLGLIAAARYALDLGIDAIAAEVRSRAERLRAG 201 Query: 312 ALE-KGFNVLG-DSQITLIVTGIEFEREKEIVR-------------KLNEMGIIVSQRGA 356 E G V ++++ IVT R+ VR L I +++RG Sbjct: 202 LREVPGVEVRDIGARLSGIVTFTVEGRDPVAVRDELARHDVVVTTSHLTSTRIDMTRRGV 261 Query: 357 KGIHGIRVSPHLYNTKEDIEKFIE 380 +G+ +R+SPH + + E +++ +E Sbjct: 262 EGL--VRMSPHYFVSPEQVDRAVE 283 >pir||F70019 nifS protein homolog yurW - Bacillus subtilis >gi|2635765|emb|CAB15258.1| (Z99120) similar to NifS protein homolog [Bacillus subtilis] Length = 406 Score = 80.8 bits (196), Expect = 3e-14 Identities = 70/270 (25%), Positives = 125/270 (45%), Gaps = 33/270 (12%) Query: 130 KNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVDAVQSLGA 189 ++G + DV + + T V S V + G P KE+++IAH++GA ++VD QS Sbjct: 146 EDGTISLEDVRETVTSNTKIVAVSHVSNVLGTVNPIKEMAKIAHDNGAVIVVDGAQSTPH 205 Query: 190 LQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPPVPWSDWW 249 +++ + L D K + P G G++Y LE +P E D+ Sbjct: 206 MKIDVQDLDCDFFALSSHK-MCGPT-GVGVLYGKKALLENMEP------AEFGGEMIDFV 257 Query: 250 GDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKEDLKLAEMVR 309 G + W LP K + GTP A+ L +++ + ++G+ +I + + KLA Sbjct: 258 G-LYESTWKELP-----WKFEAGTPIIAGAIGLGAAIDFLEEIGLDEISRHEHKLA---- 307 Query: 310 EWALEK-----GFNVLG-DSQITLIVTGIEFEREKEIVRKLNEMGIIV------SQRGAK 357 +ALE+ G V G + + L+ ++ ++ L+ GI V +Q K Sbjct: 308 AYALERFRQLDGVTVYGPEERAGLVTFNLDDVHPHDVATVLDAEGIAVRAGHHCAQPLMK 367 Query: 358 GIH---GIRVSPHLYNTKEDIEKFIEGIER 384 + R S +LYNT+E+I+K +E +++ Sbjct: 368 WLDVTATARASFYLYNTEEEIDKLVEALQK 397 >pir||A83275 hypothetical protein PA2958 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9949056|gb|AAG06346.1|AE004722_2 (AE004722) hypothetical protein [Pseudomonas aeruginosa] Length = 377 Score = 75.7 bits (183), Expect = 8e-13 Identities = 90/375 (24%), Positives = 164/375 (43%), Gaps = 37/375 (9%) Query: 14 YLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVLSEA-AKLMRTRS- 71 YLN A + P V+ F + + + A + + + +E L E A+L+ R+ Sbjct: 16 YLNHAAVAPWPRRAADAVSAFARENVAH----GASDYPEWLKIEHRLRERLARLLNARAG 71 Query: 72 RNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKHNLKVEVIE-SK 130 ++ L T++ L L+ ++ +++ + EFP+ V ++ + VEV+E Sbjct: 72 SDLALVKNTSEALSFVAFGLDWREGDQVVIGNEEFPSNRVVWEALRPRG---VEVVEVGL 128 Query: 131 NGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVDAVQSLGAL 190 G S + A T + S VQ+ +G ++ + I G +DA+Q LGAL Sbjct: 129 AGDDPESALLAACGPRTRLLSISAVQYASGLRLDLERIGAGCRRRGVLFCIDAIQQLGAL 188 Query: 191 QVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPPVPWSDWWG 250 + +A G KW+L P G G+ Y E + + L W Sbjct: 189 PFDVQAYDCAFAMADGYKWMLGPE-GLGVFYCH----PEQRERLAL----HEYGWHMLEH 239 Query: 251 DKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGIKKIEKEDLKLAEMVRE 310 D + D P R AR+ + G+P L A+AL+ SL L+ +VG++++ ++ +AE V + Sbjct: 240 ALDYERRDWRPAR-SARRFECGSPNMLGAMALEASLGLLEEVGMERVGRD---IAERV-Q 294 Query: 311 WALEKGFNVLG---------DSQITLIVTGIEFEREKEIVRKLNEMGIIVSQRGAKGIHG 361 W + + G + ++ ++ + + + L + +I +QRG G Sbjct: 295 WLQDGLSGIPGVRLHSPRNPSRRAGILTFSLDGQDNRTLFETLRQERVICAQRGG----G 350 Query: 362 IRVSPHLYNTKEDIE 376 IR SPH Y + I+ Sbjct: 351 IRFSPHFYTSSTVID 365 >pir||A72506 probable tRNA splicing protein SPL1 APE2023 - Aeropyrum pernix (strain K1) >gi|5105721|dbj|BAA81033.1| (AP000063) 411aa long hypothetical tRNA splicing protein SPL1 [Aeropyrum pernix] Length = 411 Score = 74.5 bits (180), Expect = 2e-12 Identities = 94/418 (22%), Positives = 177/418 (41%), Gaps = 51/418 (12%) Query: 1 MRELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLL-DVIKYKEGINAVEILDPMYLEPV 59 +R FP L R YL+ A L P V++ + EF G++ + + E Sbjct: 9 VRSEFPELERGIVYLDNAASTLKPRRVVEAMREFSYRSYANVHRGVHRLSMEASKAYEDA 68 Query: 60 LSEAAKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMK-----IISFDLEFPTISAVVK 114 A+L+ V + TT+ ++ A L L ++ + + D + V Sbjct: 69 HEVVARLVGGSWDEVVFTRNTTEAMQLAALTLAYNGLLRGGEVLVTAADHHSTLLPWVRA 128 Query: 115 SYAEKHNLKVEVIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHE 174 + ++ ++ + G+ +E I ++T AV V +TG P ++I + A + Sbjct: 129 ARLGGGRARILPLDGR-GVPRWDLLEDYITEDTRAVAVGHVSNVTGVVAPVEDIVKAAKK 187 Query: 175 HGAWVIVDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPII 234 GA V++D+ Q + L V ++GVD+ G K +L P G G+++ LEE +P + Sbjct: 188 VGALVVLDSAQGVPHLPVDFRRMGVDMAAFSGHK-MLGPT-GIGVLWARRDLLEELEPPL 245 Query: 235 GLLNTEPPVPWSDWWGDKDKDLWDILPVRGDARKLDPGTPAYLSAVALKPSLELINDVGI 294 G T V G + W+ P K + GTP + AV L + ++ ++G+ Sbjct: 246 GGGGTVSRVRLQ---GGSVEIEWEEPP-----WKFEAGTPPIIEAVGLAEAANMLMEIGM 297 Query: 295 KKIEKED-------LKLAEMVREWALEK--GFNVLGDSQITLIVTGIEFEREKEIVRKLN 345 + + + + +KL E + L + G + G+ + +T ++ + Sbjct: 298 EHVARHEDELTSHTMKLLEPLYSEGLIRYVGPDKPGERHGIVSIT--------THLKSPD 349 Query: 346 EMGIIVSQRG---AKGIH--------------GIRVSPHLYNTKEDIEKFIEGIERFL 386 E+G+++ +RG G+H + S ++YN KED E+ E +E L Sbjct: 350 ELGLLLDRRGIAVRTGLHCAHILHDHVDASMGSVWASFYIYNCKEDAERLAEALEEIL 407 >gi|11499012 aspartate aminotransferase (aspC) [Archaeoglobus fulgidus] >gi|7434112|pir||H69426 probable transaminase (EC 2.6.1.-) AF1417 [similarity] - Archaeoglobus fulgidus >gi|2649155|gb|AAB89830.1| (AE001006) aspartate aminotransferase (aspC) [Archaeoglobus fulgidus] Length = 372 Score = 73.0 bits (176), Expect = 6e-12 Identities = 72/281 (25%), Positives = 130/281 (45%), Gaps = 33/281 (11%) Query: 81 TDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKHNLKVEVIESKNGLYDISDVE 140 T G++ A+ + K + + + + + Y + +KV ES +++ V+ Sbjct: 60 TAGMEAAIASFSRVKKIATLENGKFGERLGDIAERYTQVERVKVPWGES----FELDAVK 115 Query: 141 KAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVDAVQSLGALQVYPEKLGVD 200 +A+D+ AV F + TG P KEI+++A E+ A VI+DA+ S G V ++ GVD Sbjct: 116 EALDNGCEAVAFVHNETSTGILNPAKEIAKLAKEYDALVIMDAITSAGGDYVKMDEWGVD 175 Query: 201 VLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPPVPWSDWWGDKDKDLWDIL 260 V + G +K L P G + +S++ W D++ ++ D+ Sbjct: 176 VAIVGSQKCLGAP-PGLAAVAVSEK------------------AW-DYYNERCPYYLDLA 215 Query: 261 PVRGDARKLD-PGTPAYLSAVALKPSLELINDVGIKKIEKEDLKLAEMVREWALEKGFNV 319 R + + P TPA AL +L++I++ G++ + L+ VR+WA+E G + Sbjct: 216 AYRKKLKDMQTPYTPAVPLFFALAEALKIIDEEGLENRIQRHRILSAAVRKWAVEAGLEL 275 Query: 320 LGD----SQITLIVTGIEFER---EKEIVRKL-NEMGIIVS 352 + S + VT I+ + E+ L E GI+VS Sbjct: 276 FPNLNKYSSYSNTVTAIKMPEGVSDSELRGTLKKEYGILVS 316 >pir||S77167 L-cysteine desulfhydrase (EC 4.4.1.-) - Synechocystis sp. (strain PCC 6803) >gi|1652806|dbj|BAA17725.1| (D90908) isopenicillin N epimerase [Synechocystis sp.] Length = 401 Score = 70.6 bits (170), Expect = 3e-11 Identities = 58/232 (25%), Positives = 102/232 (43%), Gaps = 17/232 (7%) Query: 2 RELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVLS 61 R+ FPGL + Y N G G+LP L+ +T + Y + I Y++ +++ Sbjct: 11 RQQFPGLAN-KTYFNFGGQGILPTVALEAITA----MYGYLQENGPFSIAANQYIQQLIA 65 Query: 62 EAAKLM----RTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYA 117 + + + + ++ T G L ++ +K +I+ D E P I A+V++ A Sbjct: 66 QLRQALGETFNVDPNTITITDNVTTGCDIVLWGIDWQKGDEILLTDCEHPGIIAIVQAIA 125 Query: 118 EKHNLKVE---VIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHE 174 + + V ++ N + + + +T V+ S + W TGQ +P EI + Sbjct: 126 ARFGVTYRFFPVADTLNQGDAAAVLVNHLGPKTRLVILSHLLWNTGQVLPLGEIMAVCRR 185 Query: 175 HGA----WVIVDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYL 222 H V+VD QS G+L + +L VD G KW P G G +Y+ Sbjct: 186 HQGDYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGP-AGVGGLYI 236 >gb|AAD02935.1| (AF061964) L-cysteine/cystine lyase C-DES [Synechocystis PCC6714] Length = 393 Score = 70.2 bits (169), Expect = 4e-11 Identities = 61/242 (25%), Positives = 102/242 (41%), Gaps = 17/242 (7%) Query: 2 RELFPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVLS 61 R FPGL + Y N G G+LP L+ +T + Y + I +++ +++ Sbjct: 11 RHQFPGLAN-KTYFNFGGQGILPTVALEAITA----MYGYLQENGPFSIAANQHIQQLIA 65 Query: 62 EA----AKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYA 117 + A+ + ++ T G L L+ + +I+ D E P I A+V++ A Sbjct: 66 QLRQALAETFNVDPNTITITDNVTTGCDIVLWGLDWHQGDEILLTDCEHPGIIAIVQAIA 125 Query: 118 EKHNLKVE---VIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHE 174 + + V + N + + + +T V+ S + W TGQ +P EI + Sbjct: 126 ARFGITYRFFPVAATLNQGDAAAVLANHLGPKTRLVILSHLLWNTGQVLPLAEIMAVCRR 185 Query: 175 HGA----WVIVDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEA 230 H V+VD QS G+L + +L VD G KW P G G +Y+ L E Sbjct: 186 HQGNYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGP-AGVGGLYIHGDCLGEI 244 Query: 231 KP 232 P Sbjct: 245 NP 246 >pir||G72047 nifs-related aminotransferase - Chlamydophila pneumoniae (strain CWL029) >gi|4376991|gb|AAD18828.1| (AE001651) NifS-related Aminotransferase [Chlamydophila pneumoniae CWL029] >gi|8163356|gb|AAF73623.1| (AE002169) aminotransferase, class V [Chlamydophila pneumoniae AR39] >gi|8979061|dbj|BAA98896.1| (AP002547) NifS-related aminotransferase [Chlamydophila pneumoniae J138] Length = 406 Score = 69.1 bits (166), Expect = 8e-11 Identities = 75/307 (24%), Positives = 135/307 (43%), Gaps = 40/307 (13%) Query: 88 LLALEPKKNMKIISFDLEFPTISAVVKSYAEKHNLKVEVIESKNGLYDISDVEKAIDDET 147 +L E + + ++S+++ ++VK K+ V +S GL D+ D+EK +++ Sbjct: 113 VLVSEAEHHANVLSWEIACRRRGSLVK--------KIRVHDS--GLIDLDDLEKLLNEGA 162 Query: 148 FAVVFSDVQWITGQQMPTKEISEIAHEHGAWVIVDAVQSLGALQVYPEKLGVDVLVAGGE 207 V V +TG P ++++E+ H + A++ VD Q L + + VD V Sbjct: 163 QFVSIPHVSNVTGCVQPLQQVAELVHRYDAYLAVDGAQGAPHLPIDVQLWDVDFYVFSSH 222 Query: 208 KWLLNPNMGSGIMYLSDRFLEEAKPIIGLLNTEPPVPWSDWWGDKDKDLWDILPVRGDAR 267 K + P G G++Y L++ P+ G D D + LP Sbjct: 223 K-IYGPT-GIGVLYGKKDLLDQLPPVEG---------GGDMVAIYDHQNPEYLPA---PM 268 Query: 268 KLDPGTPAYLSAVALKPSLELINDVGIKKIEKEDLKLAEMVREWALE-KGFNVLGDS--- 323 K + GTP + L +L+ ++ + K I +++ L + + LE G +LG S Sbjct: 269 KFEAGTPNIAGVLGLGAALDYLDGLSAKFIYDKEIALTTYLHKELLEIPGVEILGPSIEE 328 Query: 324 -QITLIVTGIEFEREKEIVRKLNEMGIIV----------SQRGAKGIHGIRVSPHLYNTK 372 + LI I+ ++ L+ GI V +R G H +RVS +YN + Sbjct: 329 PRGALIGMTIDGAHPLDLGFLLDLRGIAVRTGHQCAQPAMERWNVG-HVLRVSLGIYNDE 387 Query: 373 EDIEKFI 379 +DI++FI Sbjct: 388 DDIDQFI 394 >pdb|1ELU|A Chain A, Complex Between The Cystine C-S Lyase C-Des And Its Reaction Product Cysteine Persulfide. >gi|7767012|pdb|1ELU|B Chain B, Complex Between The Cystine C-S Lyase C-Des And Its Reaction Product Cysteine Persulfide. >gi|7767009|pdb|1ELQ|A Chain A, Crystal Structure Of The Cystine C-S Lyase C-Des >gi|7767010|pdb|1ELQ|B Chain B, Crystal Structure Of The Cystine C-S Lyase C-Des Length = 390 Score = 69.1 bits (166), Expect = 8e-11 Identities = 60/239 (25%), Positives = 101/239 (42%), Gaps = 17/239 (7%) Query: 5 FPGLRRFRAYLNTAGLGLLPITVLKEVTEFLLDVIKYKEGINAVEILDPMYLEPVLSEA- 63 FPGL + Y N G G+LP L+ +T + Y + I +++ ++++ Sbjct: 11 FPGLAN-KTYFNFGGQGILPTVALEAITA----MYGYLQENGPFSIAANQHIQQLIAQLR 65 Query: 64 ---AKLMRTRSRNVGLSIQTTDGLKRALLALEPKKNMKIISFDLEFPTISAVVKSYAEKH 120 A+ + ++ T G L L+ + +I+ D E P I A+V++ A + Sbjct: 66 QALAETFNVDPNTITITDNVTTGCDIVLWGLDWHQGDEILLTDCEHPGIIAIVQAIAARF 125 Query: 121 NLKVE---VIESKNGLYDISDVEKAIDDETFAVVFSDVQWITGQQMPTKEISEIAHEHGA 177 + V + N + + + +T V+ S + W TGQ +P EI + H Sbjct: 126 GITYRFFPVAATLNQGDAAAVLANHLGPKTRLVILSHLLWNTGQVLPLAEIMAVCRRHQG 185 Query: 178 ----WVIVDAVQSLGALQVYPEKLGVDVLVAGGEKWLLNPNMGSGIMYLSDRFLEEAKP 232 V+VD QS G+L + +L VD G KW P G G +Y+ L E P Sbjct: 186 NYPVRVLVDGAQSAGSLPLDFSRLEVDYYAFTGHKWFAGP-AGVGGLYIHGDCLGEINP 243 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.318 0.139 0.401 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 143310018 Number of Sequences: 2977 Number of extensions: 6192214 Number of successful extensions: 16687 Number of sequences better than 1.0e-10: 17 Number of HSP's better than 0.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 16657 Number of HSP's gapped (non-prelim): 19 length of query: 386 length of database: 189,106,746 effective HSP length: 56 effective length of query: 330 effective length of database: 155,591,474 effective search space: 51345186420 effective search space used: 51345186420 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.6 bits) S2: 166 (69.1 bits)