BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB0950 (PAB0950) DE:TRANSCRIPTION regulator
(190 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75055 transcription regulator PAB0950 - Pyrococcus abyssi ... 387 e-107
pir||B71106 hypothetical protein PH0619 - Pyrococcus horikoshii ... 342 2e-93
sp|Q58187|Y777_METJA HYPOTHETICAL PROTEIN MJ0777 >gi|2826316|gb|... 141 4e-33
pir||A64397 hypothetical protein MJ0777 - Methanococcus jannaschii 141 4e-33
pir||A69090 conserved hypothetical protein MTH1669 - Methanobact... 123 1e-27
gi|11498363 transcription initiation factor IIE, subunit alpha, ... 111 4e-24
gb|AAK40605.1| Transcription Factor E (TFE), TFIIE alpha subunit... 107 1e-22
gb|AAG19231.1| (AE005019) transcription initiation factor IIE al... 102 2e-21
pir||F72503 hypothetical protein APE2004 - Aeropyrum pernix (str... 76 3e-13
>pir||C75055 transcription regulator PAB0950 - Pyrococcus abyssi (strain Orsay)
>gi|5458849|emb|CAB50336.1| (AJ248287) TRANSCRIPTION
regulator [Pyrococcus abyssi]
Length = 190
Score = 387 bits (984), Expect = e-107
Identities = 190/190 (100%), Positives = 190/190 (100%)
Query: 1 MSRKNKALLEIAKDIGGDEAVEIVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEK 60
MSRKNKALLEIAKDIGGDEAVEIVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEK
Sbjct: 1 MSRKNKALLEIAKDIGGDEAVEIVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEK 60
Query: 61 LADFKRIKDEETGWYYYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPE 120
LADFKRIKDEETGWYYYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPE
Sbjct: 61 LADFKRIKDEETGWYYYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPE 120
Query: 121 HPKLTFDEAFEYGFTCPICGEILQEYDNSAVIEELKKRIEELEIELGLRPSPKKEKKKTR 180
HPKLTFDEAFEYGFTCPICGEILQEYDNSAVIEELKKRIEELEIELGLRPSPKKEKKKTR
Sbjct: 121 HPKLTFDEAFEYGFTCPICGEILQEYDNSAVIEELKKRIEELEIELGLRPSPKKEKKKTR 180
Query: 181 AKAKRKTRKK 190
AKAKRKTRKK
Sbjct: 181 AKAKRKTRKK 190
>pir||B71106 hypothetical protein PH0619 - Pyrococcus horikoshii
>gi|3257025|dbj|BAA29708.1| (AP000003) 197aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 197
Score = 342 bits (867), Expect = 2e-93
Identities = 161/190 (84%), Positives = 177/190 (92%)
Query: 1 MSRKNKALLEIAKDIGGDEAVEIVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEK 60
MS++NK LLEI +DIGGDEAVEI+KALEKKGEATDEELAEITGIRVNTVRKILYALYD K
Sbjct: 7 MSKRNKELLEIGRDIGGDEAVEIIKALEKKGEATDEELAEITGIRVNTVRKILYALYDAK 66
Query: 61 LADFKRIKDEETGWYYYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPE 120
LADF+R+KD+ETGWYYYYWH+ETKRLPEIIRARK++ELE+LKKMLQEETSEVYYHCGNP+
Sbjct: 67 LADFRRVKDDETGWYYYYWHIETKRLPEIIRARKMQELEKLKKMLQEETSEVYYHCGNPD 126
Query: 121 HPKLTFDEAFEYGFTCPICGEILQEYDNSAVIEELKKRIEELEIELGLRPSPKKEKKKTR 180
HPKLTFDEAFEYGF CPICGEIL +YDNSAVIEELKKRIEELEIELGLR PKKEKK +
Sbjct: 127 HPKLTFDEAFEYGFVCPICGEILHQYDNSAVIEELKKRIEELEIELGLRAPPKKEKKGKK 186
Query: 181 AKAKRKTRKK 190
+K + K KK
Sbjct: 187 SKKRSKKSKK 196
>sp|Q58187|Y777_METJA HYPOTHETICAL PROTEIN MJ0777 >gi|2826316|gb|AAB98767.1| (U67522)
putative transcription initiation factor (TFIIE, subunit
alpha) [Methanococcus jannaschii]
Length = 191
Score = 141 bits (353), Expect = 4e-33
Identities = 64/155 (41%), Positives = 108/155 (69%), Gaps = 1/155 (0%)
Query: 16 GGDEAVEIVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEKLADFKRIKDEETGWY 75
G ++ E++ L +KGE T+EE+A+ G+++N VRK+LY LYD +L D+KR KDE+T WY
Sbjct: 35 GDEKGFEVIDVLLEKGETTEEEIAKELGVKLNVVRKLLYKLYDARLVDYKRWKDEDTNWY 94
Query: 76 YYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPEHPKLTFDEAFEYGFT 135
Y W ++LP +++ + ++ L+K L+ E + +++ C N + + TF+EA +YGF+
Sbjct: 95 SYTWLPTLEKLPYVVKKKINELIKDLEKKLEFEKNNMFFFCPNC-NVRFTFEEAMDYGFS 153
Query: 136 CPICGEILQEYDNSAVIEELKKRIEELEIELGLRP 170
CP CG +LQE+DNS +I++L+++I+ L+ EL P
Sbjct: 154 CPGCGNMLQEFDNSELIKDLEEQIKFLKEELKNNP 188
>pir||A64397 hypothetical protein MJ0777 - Methanococcus jannaschii
Length = 193
Score = 141 bits (353), Expect = 4e-33
Identities = 64/155 (41%), Positives = 108/155 (69%), Gaps = 1/155 (0%)
Query: 16 GGDEAVEIVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEKLADFKRIKDEETGWY 75
G ++ E++ L +KGE T+EE+A+ G+++N VRK+LY LYD +L D+KR KDE+T WY
Sbjct: 37 GDEKGFEVIDVLLEKGETTEEEIAKELGVKLNVVRKLLYKLYDARLVDYKRWKDEDTNWY 96
Query: 76 YYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPEHPKLTFDEAFEYGFT 135
Y W ++LP +++ + ++ L+K L+ E + +++ C N + + TF+EA +YGF+
Sbjct: 97 SYTWLPTLEKLPYVVKKKINELIKDLEKKLEFEKNNMFFFCPNC-NVRFTFEEAMDYGFS 155
Query: 136 CPICGEILQEYDNSAVIEELKKRIEELEIELGLRP 170
CP CG +LQE+DNS +I++L+++I+ L+ EL P
Sbjct: 156 CPGCGNMLQEFDNSELIKDLEEQIKFLKEELKNNP 190
>pir||A69090 conserved hypothetical protein MTH1669 - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622798|gb|AAB86141.1| (AE000925) conserved protein
[Methanobacterium thermoautotrophicum]
Length = 178
Score = 123 bits (306), Expect = 1e-27
Identities = 60/154 (38%), Positives = 98/154 (62%), Gaps = 2/154 (1%)
Query: 10 EIAKDIGGDEAVEIVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEKLADFKRIKD 69
E+ + +EA+ +++ L KG+ TDEE++E T +++N VR+ILY LYD +A +KR KD
Sbjct: 13 EVITEEEEEEAIPVLECL-MKGKVTDEEISEKTQLKLNIVRRILYRLYDAGVASYKRSKD 71
Query: 70 EETGWYYYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPEHPKLTFDEA 129
ET WY Y W E +++ E+I+ + + +L++ L+ E + +++ C N H + TFDEA
Sbjct: 72 PETQWYTYTWKFEPEKVTEMIKRKHSEIVRKLREALEFEENNMFFVCVN-GHYRFTFDEA 130
Query: 130 FEYGFTCPICGEILQEYDNSAVIEELKKRIEELE 163
E FTCP CG ++ DNS +I+E+K + E
Sbjct: 131 TEENFTCPECGSKMEFMDNSEIIQEIKDELSLYE 164
>gi|11498363 transcription initiation factor IIE, subunit alpha, putative
[Archaeoglobus fulgidus] >gi|7450075|pir||E69344
transcription initiation factor IIE alpha homolog -
Archaeoglobus fulgidus >gi|2649850|gb|AAB90479.1|
(AE001052) transcription initiation factor IIE, subunit
alpha, putative [Archaeoglobus fulgidus]
Length = 177
Score = 111 bits (276), Expect = 4e-24
Identities = 55/157 (35%), Positives = 97/157 (61%), Gaps = 4/157 (2%)
Query: 10 EIAKDIGGDEAVE---IVKALEKKGEATDEELAEITGIRVNTVRKILYALYDEKLADFKR 66
E+ ++ G A E I+ +L +GE TD++L+ GI +N +RK L+ALY+ LAD+ R
Sbjct: 11 ELLNELVGRAAGEVGLILFSLGIEGEFTDDQLSLELGIEINEIRKALFALYEIGLADYVR 70
Query: 67 IKDEETGWYYYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPEHPKLTF 126
+D+ETGW YYW + + ++++ + ++L++ ++ ETS +YY C N K+++
Sbjct: 71 RRDDETGWMEYYWRINYDKALDVLKRELEKTAKKLREKIEAETSTIYYICPN-MCVKVSY 129
Query: 127 DEAFEYGFTCPICGEILQEYDNSAVIEELKKRIEELE 163
++A E FTCP CG +L D S IE++++ + +E
Sbjct: 130 NDAMELNFTCPRCGAMLDYLDCSKAIEKIEEEVRRIE 166
>gb|AAK40605.1| Transcription Factor E (TFE), TFIIE alpha subunit homolog (tfE)
[Sulfolobus solfataricus]
Length = 178
Score = 107 bits (264), Expect = 1e-22
Identities = 53/159 (33%), Positives = 95/159 (59%), Gaps = 2/159 (1%)
Query: 9 LEIAKDIGGDEAVEIVKALEKKG-EATDEELAEITGIRVNTVRKILYALYDEKLADFKRI 67
+ +AK + GD+ +++++ L KG E TDEE+A I+VN VRK L L ++ +++
Sbjct: 9 INLAKSLLGDDVIDVLRILLDKGTEMTDEEIANQLNIKVNDVRKKLNLLEEQGFVSYRKT 68
Query: 68 KDEETGWYYYYWHLETKRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPEHPKLTFD 127
+D+++GW+ YYW ++ EI+ RK L++LK L+ E + ++ C ++ + +F+
Sbjct: 69 RDKDSGWFIYYWKPNIDQINEILLNRKRLILDKLKTRLEYEKNNTFFICPQ-DNSRYSFE 127
Query: 128 EAFEYGFTCPICGEILQEYDNSAVIEELKKRIEELEIEL 166
EAFE F C CG L YD + L+++I ++E E+
Sbjct: 128 EAFENEFKCLKCGSQLTYYDTDKIKSFLEQKIRQIEEEI 166
>gb|AAG19231.1| (AE005019) transcription initiation factor IIE alpha subunit; TfeA
[Halobacterium sp. NRC-1]
Length = 181
Score = 102 bits (253), Expect = 2e-21
Identities = 52/145 (35%), Positives = 80/145 (54%), Gaps = 1/145 (0%)
Query: 24 VKALEKKGEATDEELAEITGIRVNTVRKILYALYDEKLADFKRIKDEETGWYYYYWHLET 83
V A GE TDEELAE G+ +N VR+ L+ LY+ LA ++R++DE++GW Y W +
Sbjct: 36 VAAAPPDGEVTDEELAERLGLELNDVRRALFILYENDLATYRRVRDEDSGWLTYLWTFQY 95
Query: 84 KRLPEIIRARKLRELERLKKMLQEETSEVYYHCGNPEHPKLTFDEAFEYGFTCPICGEIL 143
+ +P +R R LE L + E +Y C + F EA + GF CP CG +
Sbjct: 96 ENVPGNLREEMDRLLEALVDRREYELENEFYLC-EVCSIRFEFGEAMDLGFECPECGSAV 154
Query: 144 QEYDNSAVIEELKKRIEELEIELGL 168
+ +N+ +++ + RIE L EL +
Sbjct: 155 EAMENTDLVDAMDDRIERLRSELAV 179
>pir||F72503 hypothetical protein APE2004 - Aeropyrum pernix (strain K1)
>gi|5105702|dbj|BAA81014.1| (AP000063) 160aa long
hypothetical protein [Aeropyrum pernix]
Length = 160
Score = 75.7 bits (183), Expect = 3e-13
Identities = 40/136 (29%), Positives = 73/136 (53%), Gaps = 1/136 (0%)
Query: 31 GEATDEELAEITGIRVNTVRKILYALYDEKLADFKRIKDEETGWYYYYWHLETKRLPEII 90
G +D++L +TG + + +R+IL L D+++ ++ + Y+W +++ + +
Sbjct: 16 GGISDDDLESLTGYKQSDIRRILRLLGDKRIIVSRKGRHPRKEATRYFWRIDSDTINVSL 75
Query: 91 RARKLRELERLKKMLQEETSEVYYHCGNPEHPKLTFDEAFEYGFTCPICGEILQEYDNSA 150
K + LE+L ++ YY C K +FDEAF FTCP CGE+L+E D+
Sbjct: 76 LTLKKKVLEKLVVKEAHDSGNSYYTCPRCGS-KYSFDEAFTLDFTCPRCGEVLEEADSRE 134
Query: 151 VIEELKKRIEELEIEL 166
+E L++ I+ L E+
Sbjct: 135 GLERLRRTIDALREEI 150
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.314 0.135 0.381
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69986138
Number of Sequences: 2977
Number of extensions: 2969036
Number of successful extensions: 13483
Number of sequences better than 1.0e-10: 9
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 13462
Number of HSP's gapped (non-prelim): 10
length of query: 190
length of database: 189,106,746
effective HSP length: 57
effective length of query: 133
effective length of database: 154,992,987
effective search space: 20614067271
effective search space used: 20614067271
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 42 (21.9 bits)
S2: 162 (67.5 bits)