BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1037 (PAB1037) DE:SIMILAR TO E. COLI GLUCOSAMINE SYNTHETASE (208 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||D75004 hypothetical protein PAB1037 - Pyrococcus abyssi (st... 440 e-123 pir||B64489 hypothetical protein MJ1515 - Methanococcus jannasch... 139 2e-32 pir||A82104 conserved hypothetical protein VC2229 [imported] - V... 127 8e-29 sp|P44098|YAFJ_HAEIN HYPOTHETICAL PROTEIN HI1037 >gi|1074620|pir... 120 2e-26 sp|Q47147|YAFJ_ECOLI HYPOTHETICAL 28.6 KD PROTEIN IN GMHA-DINJ I... 116 2e-25 pir||H81038 conserved hypothetical protein NMB1815 [imported] - ... 114 8e-25 gb|AAF61909.1|AF216815_5 (AF216815) YafJ-like protein [Azospiril... 114 1e-24 pir||F81984 hypothetical protein NMA0646 [imported] - Neisseria ... 114 1e-24 emb|CAA05723.1| (AJ002783) YafJ [Neisseria gonorrhoeae] 113 2e-24 pir||G83481 conserved hypothetical protein PA1307 [imported] - P... 109 3e-23 pir||C69062 conserved hypothetical protein MTH1466 - Methanobact... 77 2e-13 >pir||D75004 hypothetical protein PAB1037 - Pyrococcus abyssi (strain Orsay) >gi|5458988|emb|CAB50474.1| (AJ248288) SIMILAR TO E. COLI GLUCOSAMINE SYNTHETASE [Pyrococcus abyssi] Length = 208 Score = 440 bits (1120), Expect = e-123 Identities = 208/208 (100%), Positives = 208/208 (100%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSELNPHGWGVGWYLMTINGNRAASIIKQPIPTYRS 60 MCELFGVNANKDVDVNFTWRGFVRKSELNPHGWGVGWYLMTINGNRAASIIKQPIPTYRS Sbjct: 1 MCELFGVNANKDVDVNFTWRGFVRKSELNPHGWGVGWYLMTINGNRAASIIKQPIPTYRS 60 Query: 61 RIALMLPRLNIRSQIIMSHVRFATSGVSYLNTHPFVRRIWSVGQYDEWIFAHNGVLDGVE 120 RIALMLPRLNIRSQIIMSHVRFATSGVSYLNTHPFVRRIWSVGQYDEWIFAHNGVLDGVE Sbjct: 61 RIALMLPRLNIRSQIIMSHVRFATSGVSYLNTHPFVRRIWSVGQYDEWIFAHNGVLDGVE 120 Query: 121 ELPKRFKPLGTTDSEAAFCYIMENLEGIRTIKELFTKLYQLLNEVSNYGTLNILMSNGRY 180 ELPKRFKPLGTTDSEAAFCYIMENLEGIRTIKELFTKLYQLLNEVSNYGTLNILMSNGRY Sbjct: 121 ELPKRFKPLGTTDSEAAFCYIMENLEGIRTIKELFTKLYQLLNEVSNYGTLNILMSNGRY 180 Query: 181 LFAYTHYPGKGMVLLKRHPPHRGSFKTP 208 LFAYTHYPGKGMVLLKRHPPHRGSFKTP Sbjct: 181 LFAYTHYPGKGMVLLKRHPPHRGSFKTP 208 >pir||B64489 hypothetical protein MJ1515 - Methanococcus jannaschii >gi|1592147|gb|AAB99533.1| (U67592) conserved hypothetical protein [Methanococcus jannaschii] Length = 355 Score = 139 bits (348), Expect = 2e-32 Identities = 81/206 (39%), Positives = 121/206 (58%), Gaps = 14/206 (6%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSELNPHGWGVGWYLMTINGNRAASIIKQPIPTYRS 60 MCEL G+ NK V+V + F +SE +P+GWG+ +Y + +IK+PI + Sbjct: 1 MCELLGICFNKKVNVELSLNSFKHRSEDHPNGWGIAFY-----PDGFVRVIKEPIKMNEA 55 Query: 61 RIALMLPRLNIRSQIIMSHVRFATSGV-SYLNTHPFVRRIWSVGQYDEWIFAHNGVLDGV 119 +A + I+S I ++H+R A++G SY+NTHPFVR++ + E FAHNG L G Sbjct: 56 LLAECVRWTKIKSNIFIAHIRKASAGSESYVNTHPFVRKL----EDKEIAFAHNGTLLGY 111 Query: 120 EELP-KRFKPLGTTDSEAAFCYIMENLEG--IRTIKELFTKLYQLLNEVSNYGTLNILMS 176 E+L + P+G TDSE FCY++ +E I KE F ++ +L +++ YG N L S Sbjct: 112 EDLELDGYYPIGETDSEYVFCYLLSQIEKREIEWNKEGFDEMLDILLDINYYGAFNCLFS 171 Query: 177 NGRYLFAYTHYPG-KGMVLLKRHPPH 201 +G YLFAY Y G + + LKR PP+ Sbjct: 172 DGEYLFAYKDYRGRRELHFLKRKPPY 197 >pir||A82104 conserved hypothetical protein VC2229 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9656787|gb|AAF95373.1| (AE004294) conserved hypothetical protein [Vibrio cholerae] Length = 281 Score = 127 bits (317), Expect = 8e-29 Identities = 73/194 (37%), Positives = 113/194 (57%), Gaps = 19/194 (9%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKS-ELNPH--GWGVGWYLMTINGNRAASIIKQPIPT 57 MCEL G++AN D+ F++ G +++ + PH GWG+ +Y + K P P+ Sbjct: 1 MCELLGMSANVPTDICFSFTGLMQRGGKTGPHRDGWGIAFY-----EGKGFRTFKDPNPS 55 Query: 58 YRSRIALMLPRLNIRSQIIMSHVRFAT-SGVSYLNTHPFVRRIWSVGQYDEWIFAHNGVL 116 +S+IA ++ + I+S+ ++SH+R A GV+ NTHPF R +W GQY W FAHNG L Sbjct: 56 SQSKIAELVQQYPIKSRAVISHIRQANRGGVNLENTHPFTRELW--GQY--WTFAHNGQL 111 Query: 117 DGVEELPK-RFKPLGTTDSEAAFCYIMENL-----EGIRTIKELFTKLYQLLNEVSNYGT 170 G + L R +P+G TDSE AFC+++ + EG + E+ + Q +E+ G Sbjct: 112 TGYQNLVTGRHRPVGETDSELAFCWLLNQMEQCYPEGPQDWPEMLRYVAQCCDELRALGV 171 Query: 171 LNILMSNGRYLFAY 184 N+L+SNG Y+ AY Sbjct: 172 FNMLLSNGEYVMAY 185 >sp|P44098|YAFJ_HAEIN HYPOTHETICAL PROTEIN HI1037 >gi|1074620|pir||H64018 hypothetical protein HI1037 - Haemophilus influenzae (strain Rd KW20) >gi|1574070|gb|AAC22697.1| (U32784) conserved hypothetical protein [Haemophilus influenzae Rd] Length = 294 Score = 120 bits (297), Expect = 2e-26 Identities = 69/197 (35%), Positives = 114/197 (57%), Gaps = 26/197 (13%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSEL---NPHGWGVGWY----LMTINGNRAASIIKQ 53 MC+L G+N N D+ F++ GF R++ L + G+G+ ++ + N+AAS+ Sbjct: 17 MCQLLGMNCNTPTDIVFSFEGFRRRAGLTDCHSDGFGIAFFEGRGVRIFRDNQAASL--- 73 Query: 54 PIPTYRSRIALMLPRLNIRSQIIMSHVRFATSG-VSYLNTHPFVRRIWSVGQYDEWIFAH 112 S IA + + NI+S +++H+R AT G V+ NTHPF+R IW W+FAH Sbjct: 74 ------SPIADCIKQYNIKSLNVIAHIRKATQGEVNIENTHPFIREIWG----QNWVFAH 123 Query: 113 NGVLDGVEELPKRF-KPLGTTDSEAAFCYIMENLEGIRTIK----ELFTKLYQLLNEVSN 167 NG L + ++ F +P+G+TDSEAAFCY+ E L+ K E+F + ++ ++ Sbjct: 124 NGNLKNLPDMTDHFLQPIGSTDSEAAFCYMAEYLKNTFRKKPSEMEIFEAIQKVTKGLAQ 183 Query: 168 YGTLNILMSNGRYLFAY 184 +GT N ++SNG ++ A+ Sbjct: 184 HGTFNFILSNGEWMIAH 200 >sp|Q47147|YAFJ_ECOLI HYPOTHETICAL 28.6 KD PROTEIN IN GMHA-DINJ INTERGENIC REGION >gi|7429308|pir||H64746 yafJ protein - Escherichia coli >gi|984579|dbj|BAA07585.1| (D38582) YafJ [Escherichia coli] >gi|1552793|gb|AAB08645.1| (U70214) hypothetical [Escherichia coli] >gi|1786417|gb|AAC73327.1| (AE000131) putative amidotransferase [Escherichia coli K12] >gi|4902959|dbj|BAA77893.1| (D83536) Hypothetical protein HI1037 [Escherichia coli] Length = 255 Score = 116 bits (288), Expect = 2e-25 Identities = 68/194 (35%), Positives = 105/194 (54%), Gaps = 19/194 (9%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSE-LNPH--GWGVGWYLMTINGNRAASIIKQPIPT 57 MCEL G++AN D+ F++ G V++ PH GWG+ +Y + K P P+ Sbjct: 1 MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFY-----EGKGCRTFKDPQPS 55 Query: 58 YRSRIALMLPRLNIRSQIIMSHVRFATSG-VSYLNTHPFVRRIWSVGQYDEWIFAHNGVL 116 + S IA ++ I+S +++H+R A G V+ NTHPF R +W W +AHNG L Sbjct: 56 FNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWG----RNWTYAHNGQL 111 Query: 117 DGVEELPK-RFKPLGTTDSEAAFCYIMENL-----EGIRTIKELFTKLYQLLNEVSNYGT 170 G + L F+P+G TDSE AFC+++ L + +F + L +E+ G Sbjct: 112 TGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIASLADELRQKGV 171 Query: 171 LNILMSNGRYLFAY 184 N+L+S+GRY+ AY Sbjct: 172 FNMLLSDGRYVMAY 185 >pir||H81038 conserved hypothetical protein NMB1815 [imported] - Neisseria meningitidis (group B strain MD58) >gi|7227068|gb|AAF42150.1| (AE002531) conserved hypothetical protein [Neisseria meningitidis MC58] Length = 280 Score = 114 bits (283), Expect = 8e-25 Identities = 66/193 (34%), Positives = 108/193 (55%), Gaps = 18/193 (9%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSELNPH---GWGVGWYLMTINGNRAASIIKQPIPT 57 MC+L G+N N D+ F++ GF R+ + H G+G+G++ + + P+ Sbjct: 1 MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFF-----EGKGVRLFHDDKPS 55 Query: 58 YRSRIALMLPRLNIRSQIIMSHVRFATSG-VSYLNTHPFVRRIWSVGQYDEWIFAHNG-V 115 S +A ++ I+S+ +++H+R A+ G S NTHPF+R +W G Y W+FAHNG + Sbjct: 56 VNSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMW--GGY--WLFAHNGHL 111 Query: 116 LDGVEELPKRFKPLGTTDSEAAFCYIMENLEGIRTIK----ELFTKLYQLLNEVSNYGTL 171 +D E + F P+GTTDSE AFC+I+ L + LF + L +E+ +G Sbjct: 112 IDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAGLTHEIRKFGLF 171 Query: 172 NILMSNGRYLFAY 184 N ++S+G LFA+ Sbjct: 172 NFMLSDGIALFAH 184 >gb|AAF61909.1|AF216815_5 (AF216815) YafJ-like protein [Azospirillum brasilense] Length = 249 Score = 114 bits (282), Expect = 1e-24 Identities = 65/188 (34%), Positives = 105/188 (55%), Gaps = 19/188 (10%) Query: 7 VNANKDVDVNFTWRGFVRKS-ELNPH--GWGVGWYLMTINGNRAASIIKQPIPTYRSRIA 63 ++AN D+ F++RG +R+ + PH GWG+ +Y + P P+ S IA Sbjct: 1 MSANVPTDICFSFRGLMRRGGQTGPHRDGWGIAFY-----EGKGCRTFHDPAPSCESEIA 55 Query: 64 LMLPRLNIRSQIIMSHVRFATSG-VSYLNTHPFVRRIWSVGQYDEWIFAHNGVLDGVEEL 122 ++ +I+S +++SH+R A G VS NTHPF R +W W FAHNG L G++E Sbjct: 56 RLVSSYSIKSCVVISHIRRANRGRVSLENTHPFTRELWG----RVWTFAHNGQLKGIKER 111 Query: 123 PKRF-KPLGTTDSEAAFCYIMENL-----EGIRTIKELFTKLYQLLNEVSNYGTLNILMS 176 F +P+GTTDSE AFC++++ + E ++ L + L ++ G N+L+S Sbjct: 112 TLTFYEPVGTTDSEHAFCWLLDQIRMQYPEPPKSSGALMRLIRDLATDLGTLGVFNMLLS 171 Query: 177 NGRYLFAY 184 +GRYL+ + Sbjct: 172 DGRYLWCH 179 >pir||F81984 hypothetical protein NMA0646 [imported] - Neisseria meningitidis (group A strain Z2491) >gi|7379377|emb|CAB83935.1| (AL162753) hypothetical protein NMA0646 [Neisseria meningitidis Z2491] Length = 280 Score = 114 bits (282), Expect = 1e-24 Identities = 66/193 (34%), Positives = 108/193 (55%), Gaps = 18/193 (9%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSELNPH---GWGVGWYLMTINGNRAASIIKQPIPT 57 MC+L G+N N D+ F++ GF R+ + H G+G+G++ + + P+ Sbjct: 1 MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFF-----EGKGVRLFHDDKPS 55 Query: 58 YRSRIALMLPRLNIRSQIIMSHVRFATSG-VSYLNTHPFVRRIWSVGQYDEWIFAHNG-V 115 S +A ++ I+S+ +++H+R A+ G S NTHPF+R +W G Y W+FAHNG + Sbjct: 56 ANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMW--GGY--WLFAHNGHL 111 Query: 116 LDGVEELPKRFKPLGTTDSEAAFCYIMENLEGIRTIK----ELFTKLYQLLNEVSNYGTL 171 +D E + F P+GTTDSE AFC+I+ L + LF + L +E+ +G Sbjct: 112 IDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAGLTHEIRKFGLF 171 Query: 172 NILMSNGRYLFAY 184 N ++S+G LFA+ Sbjct: 172 NFMLSDGIALFAH 184 >emb|CAA05723.1| (AJ002783) YafJ [Neisseria gonorrhoeae] Length = 280 Score = 113 bits (279), Expect = 2e-24 Identities = 67/193 (34%), Positives = 109/193 (55%), Gaps = 18/193 (9%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSELNPH---GWGVGWYLMTINGNRAASIIKQPIPT 57 MC+L G+N N D+ F++ GF R+ + H G+G+G++ + + P+ Sbjct: 1 MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFF-----EGKGVRLFHDDKPS 55 Query: 58 YRSRIALMLPRLNIRSQIIMSHVRFATSG-VSYLNTHPFVRRIWSVGQYDEWIFAHNG-V 115 S +A ++ I+S+ +++H+R A+ G S NTHPF+R +W G Y W+FAHNG + Sbjct: 56 ANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMW--GGY--WLFAHNGHL 111 Query: 116 LDGVEELPKRFKPLGTTDSEAAFCYIMENLEG--IRTIKE--LFTKLYQLLNEVSNYGTL 171 +D E + F P+GTTDSE AFC+I+ L R + LF + L +E+ +G Sbjct: 112 VDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFARPSRHDTLFDAIAGLTHEIRKFGLF 171 Query: 172 NILMSNGRYLFAY 184 N ++S+G LFA+ Sbjct: 172 NFMLSDGISLFAH 184 >pir||G83481 conserved hypothetical protein PA1307 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9947244|gb|AAG04696.1|AE004560_4 (AE004560) conserved hypothetical protein [Pseudomonas aeruginosa] Length = 257 Score = 109 bits (270), Expect = 3e-23 Identities = 64/192 (33%), Positives = 103/192 (53%), Gaps = 17/192 (8%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSE-LNPH--GWGVGWYLMTINGNRAASIIKQPIPT 57 MCEL G++AN D+ F++ G +++ PH GWG+ +Y R + + P+ + Sbjct: 1 MCELLGMSANVPTDIVFSFTGLMQRGGGTGPHRDGWGIAFY-----EGRGVRLFQDPLAS 55 Query: 58 YRSRIALMLPRLNIRSQIIMSHVRFATSG-VSYLNTHPFVRRIWSVGQYDEWIFAHNGVL 116 S +A ++ R I+S+ ++ H+R A G V NTHPF+R + G+Y W FAHNG L Sbjct: 56 VDSEVARLVQRFPIKSETVIGHIRQANVGKVGLSNTHPFIRELG--GRY--WTFAHNGQL 111 Query: 117 DGVEELPKRFKPLGTTDSEAAFCYIMENLEGI----RTIKELFTKLYQLLNEVSNYGTLN 172 + P ++P+G TDSEAAFC ++ + ++ L L +E G N Sbjct: 112 ADFQPKPGFYRPVGETDSEAAFCDLLNRVRRAFPEPVPVEVLLPVLISACDEYRKKGVFN 171 Query: 173 ILMSNGRYLFAY 184 L+S+G +LF + Sbjct: 172 ALISDGDWLFTF 183 >pir||C69062 conserved hypothetical protein MTH1466 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|726052|gb|AAA87414.1| (U19362) unknown [Methanobacterium thermoautotrophicum] >gi|2622581|gb|AAB85941.1| (AE000908) conserved protein [Methanobacterium thermoautotrophicum] Length = 128 Score = 76.9 bits (186), Expect = 2e-13 Identities = 40/96 (41%), Positives = 64/96 (66%), Gaps = 7/96 (7%) Query: 1 MCELFGVNANKDVDVNFTWRGFVRKSELNPHGWGVGWYLMTINGNRAASIIKQPIPTYRS 60 +CEL G+ N++V +F+ RGF +SE NPHGWG+ +Y +++ + K+P+ + +S Sbjct: 21 LCELLGMCFNQEVQPSFSLRGFRMRSERNPHGWGLAFY-----PDKSVQVYKEPVKSRKS 75 Query: 61 RIALMLPRLN-IRSQIIMSHVRFATSG-VSYLNTHP 94 +A ++ N IRS IIMSHVR +SG +S+ +THP Sbjct: 76 IMAEIMEGYNLIRSSIIMSHVRTKSSGEISHKDTHP 111 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.324 0.140 0.445 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 81119917 Number of Sequences: 2977 Number of extensions: 3178093 Number of successful extensions: 6591 Number of sequences better than 1.0e-10: 11 Number of HSP's better than 0.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 6549 Number of HSP's gapped (non-prelim): 12 length of query: 208 length of database: 189,106,746 effective HSP length: 49 effective length of query: 159 effective length of database: 159,780,883 effective search space: 25405160397 effective search space used: 25405160397 T: 11 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (22.0 bits) S2: 163 (67.9 bits)