BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1099 (endA) DE:tRNA intron endonuclease (endA)
(170 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75017 tRNA intron endonuclease (enda) PAB1099 - Pyrococcus... 349 9e-96
pir||H71454 hypothetical protein PH0295 - Pyrococcus horikoshii ... 313 9e-85
sp|Q58819|ENDA_METJA TRNA-INTRON ENDONUCLEASE >gi|2128850|pir||G... 147 6e-35
pdb|1A79|A Chain A, Crystal Structure Of The Trna Splicing Endon... 145 2e-34
sp|O07165|ENDA_METTH PUTATIVE TRNA-INTRON ENDONUCLEASE >gi|74466... 132 3e-30
gb|AAK40764.1| tRNA intron endonuclease, putative [Sulfolobus so... 98 5e-20
gi|11498505 tRNA intron endonuclease (endA) [Archaeoglobus fulgi... 96 2e-19
gb|AAG20339.1| (AE005107) tRNA intron endonuclease; EndA [Haloba... 93 2e-18
pir||B72545 probable tRNA intron endonuclease APE1646 - Aeropyru... 91 6e-18
sp|O07118|ENDA_HALVO TRNA-INTRON ENDONUCLEASE >gi|11357105|pir||... 73 2e-12
>pir||E75017 tRNA intron endonuclease (enda) PAB1099 - Pyrococcus abyssi
(strain Orsay) >gi|5459093|emb|CAB50579.1| (AJ248288)
tRNA intron endonuclease (endA) [Pyrococcus abyssi]
Length = 170
Score = 349 bits (887), Expect = 9e-96
Identities = 170/170 (100%), Positives = 170/170 (100%)
Query: 1 MKKVIEFYLSGDRVYSTREKAINQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDE 60
MKKVIEFYLSGDRVYSTREKAINQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDE
Sbjct: 1 MKKVIEFYLSGDRVYSTREKAINQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDE 60
Query: 61 DRELTFEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNAEHSDWL 120
DRELTFEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNAEHSDWL
Sbjct: 61 DRELTFEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNAEHSDWL 120
Query: 121 IWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIKF 170
IWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIKF
Sbjct: 121 IWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIKF 170
>pir||H71454 hypothetical protein PH0295 - Pyrococcus horikoshii
>gi|3256684|dbj|BAA29367.1| (AP000001) 170aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 170
Score = 313 bits (793), Expect = 9e-85
Identities = 145/170 (85%), Positives = 163/170 (95%)
Query: 1 MKKVIEFYLSGDRVYSTREKAINQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDE 60
MKK IEFYLSGDRVYSTREKAINQLYNNRGYGELKG+KLFLSLIEAAYL E+GWIKV+D+
Sbjct: 1 MKKPIEFYLSGDRVYSTREKAINQLYNNRGYGELKGDKLFLSLIEAAYLTEKGWIKVVDK 60
Query: 61 DRELTFEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNAEHSDWL 120
D+EL F+++ KLGK +DEDFDIKY+VYKDLRDRGYIVKSALKFGSH+RVYRK+AEHSDWL
Sbjct: 61 DKELKFDDLMKLGKSRDEDFDIKYIVYKDLRDRGYIVKSALKFGSHYRVYRKDAEHSDWL 120
Query: 121 IWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIKF 170
IWVLRE+++LSPND+TAR RVAHGVRKNMV+AIVDED DVVYYK+EWIKF
Sbjct: 121 IWVLRENQRLSPNDITARARVAHGVRKNMVLAIVDEDGDVVYYKVEWIKF 170
>sp|Q58819|ENDA_METJA TRNA-INTRON ENDONUCLEASE >gi|2128850|pir||G64477 hypothetical
protein MJ1424 - Methanococcus jannaschii
>gi|1592074|gb|AAB99435.1| (U67583) tRNA intron
endonuclease (endA) [Methanococcus jannaschii]
Length = 179
Score = 147 bits (368), Expect = 6e-35
Identities = 79/174 (45%), Positives = 115/174 (65%), Gaps = 6/174 (3%)
Query: 1 MKKVIEFYLSGDRVYSTREKAINQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDE 60
M K I L GDRV + I++L + R YG ++GN L LSL+EA YL+ GW++V +
Sbjct: 6 MGKKITGLLDGDRVIVFDKNGISKL-SARHYGNVEGNFLSLSLVEALYLINLGWLEVKYK 64
Query: 61 DRE-LTFEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNA----E 115
D + L+FEE+++ + +E +KYLVYKDLR RGYIVK+ LK+G+ FR+Y + A E
Sbjct: 65 DNKPLSFEELYEYARNVEERLCLKYLVYKDLRTRGYIVKTGLKYGADFRLYERGANIDKE 124
Query: 116 HSDWLIWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIK 169
HS +L+ V E +++T VRVAH VRK +++AIVD D D+VYY + ++K
Sbjct: 125 HSVYLVKVFPEDSSFLLSELTGFVRVAHSVRKKLLIAIVDADGDIVYYNMTYVK 178
>pdb|1A79|A Chain A, Crystal Structure Of The Trna Splicing Endonuclease From
Methanococcus Jannaschii >gi|5107418|pdb|1A79|B Chain B,
Crystal Structure Of The Trna Splicing Endonuclease From
Methanococcus Jannaschii >gi|5107419|pdb|1A79|C Chain C,
Crystal Structure Of The Trna Splicing Endonuclease From
Methanococcus Jannaschii >gi|5107420|pdb|1A79|D Chain D,
Crystal Structure Of The Trna Splicing Endonuclease From
Methanococcus Jannaschii
Length = 171
Score = 145 bits (363), Expect = 2e-34
Identities = 76/166 (45%), Positives = 112/166 (66%), Gaps = 6/166 (3%)
Query: 9 LSGDRVYSTREKAINQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDEDRE-LTFE 67
L GDRV + I++L + R YG ++GN L LSL+EA YL+ GW++V +D + L+FE
Sbjct: 6 LDGDRVIVFDKNGISKL-SARHYGNVEGNFLSLSLVEALYLINLGWLEVKYKDNKPLSFE 64
Query: 68 EIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNA----EHSDWLIWV 123
E+++ + +E +KYLVYKDLR RGYIVK+ LK+G+ FR+Y + A EHS +L+ V
Sbjct: 65 ELYEYARNVEERLCLKYLVYKDLRTRGYIVKTGLKYGADFRLYERGANIDKEHSVYLVKV 124
Query: 124 LRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIK 169
E +++T VRVAH VRK +++AIVD D D+VYY + ++K
Sbjct: 125 FPEDSSFLLSELTGFVRVAHSVRKKLLIAIVDADGDIVYYNMTYVK 170
>sp|O07165|ENDA_METTH PUTATIVE TRNA-INTRON ENDONUCLEASE >gi|7446670|pir||D69131 tRNA
intron endonuclease - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2160790|gb|AAC45445.1| (AF001577) putative tRNA
intron endonuclease [Methanobacterium
thermoautotrophicum] >gi|2621300|gb|AAB84756.1|
(AE000812) tRNA intron endonuclease [Methanobacterium
thermoautotrophicum]
Length = 170
Score = 132 bits (328), Expect = 3e-30
Identities = 70/165 (42%), Positives = 111/165 (66%), Gaps = 9/165 (5%)
Query: 11 GDRVYSTREKAI-NQLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDEDRELTFEE- 68
GD V + + +I +L+ YG++ ++L LSLIEAAYL+ERG +K++ +D E++ EE
Sbjct: 8 GDEVVTIKATSIARRLHGKSHYGKMYEDRLQLSLIEAAYLMERGKLKLMKDDDEVSPEEF 67
Query: 69 IFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNA----EHSDWLIWVL 124
I LG+R KYLVY+DLR+RGYIVK+ K+G+ FR+Y + HS +L+ V+
Sbjct: 68 ISLLGER---GLYSKYLVYRDLRNRGYIVKTGFKYGAEFRLYERGGAPGRTHSAYLVRVI 124
Query: 125 RESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIK 169
E++ + D ++ VRVAHGV K ++MA +D++ D+ YY ++WI+
Sbjct: 125 SENDTIHALDFSSYVRVAHGVNKKLLMAFLDDEEDITYYLVDWIR 169
>gb|AAK40764.1| tRNA intron endonuclease, putative [Sulfolobus solfataricus]
Length = 182
Score = 98.3 bits (241), Expect = 5e-20
Identities = 57/181 (31%), Positives = 107/181 (58%), Gaps = 15/181 (8%)
Query: 4 VIEFYLSGDRVYSTREKAINQLYNNRGYGEL------KGNK-----LFLSLIEAAYLVER 52
+++ L G +V LY+N YG+ KG K L LSLIE+ YL ++
Sbjct: 1 MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKK 60
Query: 53 GWIKVLDEDRELT-FEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYR 111
G I V+D++ +L ++++++ K F+I Y VY+DLR++G+IV+S +K+G+ F VY
Sbjct: 61 GLINVVDKNGDLLEYKKLYEYSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYT 120
Query: 112 --KNAEHSDWLIWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDND-VVYYKIEWI 168
EH+ +++ + E+++P+++ + RV+H +K +V+A+VD ++ + Y +W+
Sbjct: 121 LGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRLVLALVDRKSEGIRYIMFKWV 180
Query: 169 K 169
K
Sbjct: 181 K 181
>gi|11498505 tRNA intron endonuclease (endA) [Archaeoglobus fulgidus]
>gi|3913569|sp|O29362|ENDA_ARCFU PUTATIVE TRNA-INTRON
ENDONUCLEASE >gi|7483999|pir||D69362 tRNA intron
endonuclease (endA) homolog - Archaeoglobus fulgidus
>gi|2649697|gb|AAB90338.1| (AE001041) tRNA intron
endonuclease (endA) [Archaeoglobus fulgidus]
Length = 305
Score = 96.3 bits (236), Expect = 2e-19
Identities = 52/134 (38%), Positives = 86/134 (63%), Gaps = 10/134 (7%)
Query: 24 QLYNNRGYGELKGNKLFLSLIEAAYLVERGWIKVLDEDRELTFEEIFKLGKRKDEDFDIK 83
++++ YG KG+ + LSLIE+ YL++ G + +L+ DRE E+ K + + +FD +
Sbjct: 164 EIFSRYFYGSEKGDLVTLSLIESLYLLDLGKLNLLNADRE----ELVKRAREVERNFDRR 219
Query: 84 YLVYKDLRDRGYIVKSALKFGSHFRVYRK-----NAEHSDWLIWVLRESEKLSPNDMTAR 138
Y VY++L++RG++VK+ KFGS FRVYRK + HS++L+ + +S ++ D+
Sbjct: 220 YEVYRNLKERGFVVKTGFKFGSEFRVYRKVESVDDLPHSEYLV-DIADSREIRLIDLARA 278
Query: 139 VRVAHGVRKNMVMA 152
VR+A VRK MV A
Sbjct: 279 VRLAQNVRKRMVFA 292
>gb|AAG20339.1| (AE005107) tRNA intron endonuclease; EndA [Halobacterium sp. NRC-1]
Length = 343
Score = 93.2 bits (228), Expect = 2e-18
Identities = 55/150 (36%), Positives = 87/150 (57%), Gaps = 16/150 (10%)
Query: 24 QLYNNRGYGELKGNK------LFLSLIEAAYLVERGWIKVLDEDRELTFEEIFKLGKRKD 77
+L+N YG+ G + L LSL+EAAYL++ G +++ D T +++ G+ +
Sbjct: 188 RLHNAGFYGQPLGGRAADHDALQLSLVEAAYLIDAGVLRLTDA----TIDDVRARGRLGE 243
Query: 78 -EDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRKNAE-----HSDWLIWVLRESEKLS 131
E FD + VY+ LRD G + K+ KFG+ FRVY + HS+ L+ V+ ++ S
Sbjct: 244 GEHFDCRLAVYRALRDAGAVPKTGFKFGADFRVYSAVSSVDDLGHSELLVRVITDTHVFS 303
Query: 132 PNDMTARVRVAHGVRKNMVMAIVDEDNDVV 161
P D++ VR+AHGVRK MV A D+ +D +
Sbjct: 304 PGDLSLDVRLAHGVRKRMVFAATDDTDDTI 333
>pir||B72545 probable tRNA intron endonuclease APE1646 - Aeropyrum pernix
(strain K1) >gi|5105334|dbj|BAA80647.1| (AP000062) 187aa
long hypothetical tRNA intron endonuclease [Aeropyrum
pernix]
Length = 187
Score = 91.3 bits (223), Expect = 6e-18
Identities = 59/173 (34%), Positives = 88/173 (50%), Gaps = 13/173 (7%)
Query: 9 LSGDRVYSTREKAINQLYNNRGYGE-LKGNK---------LFLSLIEAAYLVERGWIKVL 58
L GD V T + LY+ YG+ L K L LSLIE+ YL E+G ++V
Sbjct: 15 LIGDSVLVTDVEQARSLYSCGYYGQPLDVEKPRGADFEGPLRLSLIESLYLAEKGVLEVA 74
Query: 59 DEDRELTFEEIFKLGKRKDEDFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYR--KNAEH 116
D E + R + F + Y +Y+DLR+RG++V+S LKFGS F VYR +H
Sbjct: 75 KPDGSSVGVEDLRTAVRGNPRFSMLYNIYRDLRERGFVVRSGLKFGSDFAVYRLGPGIDH 134
Query: 117 SDWLIWVLRESEKLSPNDMTARVRVAHGVRKNMVMAIVDEDNDVVYYKIEWIK 169
+ +++ + + P ++ R++H VRK V A V DV Y I+W +
Sbjct: 135 APFIVHAYSPEDNIDPVEIVRAGRLSHSVRKKFVFA-VTRGGDVSYLMIDWFR 186
>sp|O07118|ENDA_HALVO TRNA-INTRON ENDONUCLEASE >gi|11357105|pir||T44993 tRNA-intron
endonuclease (EC 3.1.27.9) [validated] - Haloferax
volcanii >gi|2160792|gb|AAC45446.1| (AF001578) tRNA
intron endonuclease [Haloferax volcanii]
Length = 339
Score = 73.0 bits (176), Expect = 2e-12
Identities = 48/147 (32%), Positives = 75/147 (50%), Gaps = 14/147 (9%)
Query: 26 YNNRGYGE-LKGNKLFLSLIEAAYLVERGWIKVLDEDRELTFEEIFKLGKRKDED-FDIK 83
+ R YG L LSL+EAAYL + + + D + G+ + D FD +
Sbjct: 195 FGQRLYGRNADSGPLQLSLLEAAYLARADALAIDEAD-------VVSRGRDVEGDRFDRR 247
Query: 84 YLVYKDLRDRGYIVKSALKFGSHFRVYRK-----NAEHSDWLIWVLRESEKLSPNDMTAR 138
VY LR+ + KS KFGS FRVY + + HS++L+ V+ P D++
Sbjct: 248 LAVYAALREAKTVPKSGFKFGSDFRVYTEFESVDDLSHSEFLVRVVAPDHTFVPRDLSLD 307
Query: 139 VRVAHGVRKNMVMAIVDEDNDVVYYKI 165
VR+A GVRK MV A+ D++ ++ + +
Sbjct: 308 VRLAGGVRKRMVFALTDDNGEIDWLSV 334
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.140 0.414
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64217759
Number of Sequences: 2977
Number of extensions: 2498410
Number of successful extensions: 5701
Number of sequences better than 1.0e-10: 10
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 5677
Number of HSP's gapped (non-prelim): 15
length of query: 170
length of database: 189,106,746
effective HSP length: 52
effective length of query: 118
effective length of database: 157,985,422
effective search space: 18642279796
effective search space used: 18642279796
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 162 (67.5 bits)