BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1105 (rpoE1) DE:DNA-directed RNA polymerase, subunit E'
(rpoE1)
(188 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G75018 DNA-directed RNA polymerase, chain E' (rpoe1) PAB110... 379 e-104
pir||B71205 probable DNA-directed RNA polymerase subunit E - Pyr... 366 e-101
sp|Q57840|RPE1_METJA DNA-DIRECTED RNA POLYMERASE SUBUNIT E' >gi|... 219 2e-56
pir||C69133 DNA-dependent RNA polymerase, subunit E' - Methanoba... 200 1e-50
gi|11498717 DNA-directed RNA polymerase, subunit E' (rpoE1) [Arc... 187 8e-47
gb|AAG20209.1| (AE005097) DNA-directed RNA polymerase subunit E'... 174 6e-43
gb|AAK40742.1| DNA-directed RNA polymerase, subunit E' (rpoE1) [... 168 3e-41
sp|P39466|RPOE_SULAC DNA-DIRECTED RNA POLYMERASE SUBUNIT E >gi|4... 155 4e-37
emb|CAC12217.1| (AL445066) DNA-directed RNA polymerase, subunit ... 152 4e-36
pir||A72784 probable DNA-directed RNA polymerase subunit E' APE0... 132 2e-30
>pir||G75018 DNA-directed RNA polymerase, chain E' (rpoe1) PAB1105 - Pyrococcus
abyssi (strain Orsay) >gi|5459103|emb|CAB50589.1|
(AJ248288) DNA-directed RNA polymerase, subunit E'
(rpoE1) [Pyrococcus abyssi]
Length = 188
Score = 379 bits (962), Expect = e-104
Identities = 188/188 (100%), Positives = 188/188 (100%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV
Sbjct: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY
Sbjct: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK
Sbjct: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
Query: 181 RKEKEGKK 188
RKEKEGKK
Sbjct: 181 RKEKEGKK 188
>pir||B71205 probable DNA-directed RNA polymerase subunit E - Pyrococcus
horikoshii >gi|3258350|dbj|BAA31033.1| (AP000007) 188aa
long hypothetical DNA-directed RNA polymerase subunit E
[Pyrococcus horikoshii]
Length = 188
Score = 366 bits (930), Expect = e-101
Identities = 179/188 (95%), Positives = 184/188 (97%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLR+TYEG YD+DEGVILSIVEVKEVGDGIIV
Sbjct: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRDTYEGTYDRDEGVILSIVEVKEVGDGIIV 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
PGDGATYHEVVFDVLVWEPK+ EVVEGTVVD VPFGAF+RIGPMDGLVHISQLMDDYVVY
Sbjct: 61 PGDGATYHEVVFDVLVWEPKRDEVVEGTVVDVVPFGAFIRIGPMDGLVHISQLMDDYVVY 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
DERNKQFVGKEKKYLLKIGDAVRARIITVS KSR+IRENRIGLTMRQPGLGKFEWIEKEK
Sbjct: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSPKSRVIRENRIGLTMRQPGLGKFEWIEKEK 180
Query: 181 RKEKEGKK 188
RKEKEGKK
Sbjct: 181 RKEKEGKK 188
>sp|Q57840|RPE1_METJA DNA-DIRECTED RNA POLYMERASE SUBUNIT E' >gi|2127868|pir||E64349
DNA-directed RNA polymerase (EC 2.7.7.6) subunit E' -
Methanococcus jannaschii >gi|1591102|gb|AAB98387.1|
(U67492) DNA-directed RNA polymerase, subunit E' (rpoE1)
[Methanococcus jannaschii]
Length = 187
Score = 219 bits (552), Expect = 2e-56
Identities = 101/184 (54%), Positives = 138/184 (74%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
MYKI+ + DVV++PP F D KE +L E YEG+ DKD G +LSIV+VK++G+G +V
Sbjct: 1 MYKILEIADVVKVPPEEFGKDLKETVKKILMEKYEGRLDKDVGFVLSIVDVKDIGEGKVV 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
GDG+ YH VVF+ LV+ P+ E++EG VVD V FG+FVR+GP+DGL+H+SQ+MDDYV Y
Sbjct: 61 HGDGSAYHPVVFETLVYIPEMYELIEGEVVDVVEFGSFVRLGPLDGLIHVSQIMDDYVSY 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
D + + +GKE +L+IGD VRARI+ +S K+ R ++I LTMRQP LGK EWIE+EK
Sbjct: 121 DPKREAIIGKETGKVLEIGDYVRARIVAISLKAERKRGSKIALTMRQPYLGKLEWIEEEK 180
Query: 181 RKEK 184
K++
Sbjct: 181 AKKQ 184
>pir||C69133 DNA-dependent RNA polymerase, subunit E' - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621314|gb|AAB84770.1| (AE000812) DNA-dependent RNA
polymerase, subunit E' [Methanobacterium
thermoautotrophicum]
Length = 184
Score = 200 bits (503), Expect = 1e-50
Identities = 97/186 (52%), Positives = 130/186 (69%), Gaps = 3/186 (1%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
MY + + D VRIPP F +E A+ VL ETY G+ DK+ G ++++ E++++G G ++
Sbjct: 1 MYYVTKIVDTVRIPPDRFEEPLEEVAVEVLNETYVGRMDKNLGQLITVKEIEDIGIGKVI 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
GDGA YHEV F L ++P+ E+VEG V++ FGAFVRIGP+DGLVH+SQ+ DDY+ Y
Sbjct: 61 MGDGAAYHEVTFTALFFKPELHEIVEGEVIEIAEFGAFVRIGPVDGLVHVSQVTDDYITY 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIREN-RIGLTMRQPGLGKFEWIEKE 179
D + GKE L GD VRARI+ +S K R RE ++GLTMRQPGLGKFEWIE+E
Sbjct: 121 DPKKGSLTGKESGRRLDEGDLVRARIVALSLKGR--REGVKVGLTMRQPGLGKFEWIEEE 178
Query: 180 KRKEKE 185
KRK K+
Sbjct: 179 KRKSKK 184
>gi|11498717 DNA-directed RNA polymerase, subunit E' (rpoE1) [Archaeoglobus
fulgidus] >gi|7447212|pir||D69389 DNA-directed RNA
polymerase, subunit E' (rpoE1) homolog - Archaeoglobus
fulgidus >gi|2649472|gb|AAB90126.1| (AE001027)
DNA-directed RNA polymerase, subunit E' (rpoE1)
[Archaeoglobus fulgidus]
Length = 189
Score = 187 bits (470), Expect = 8e-47
Identities = 87/182 (47%), Positives = 129/182 (70%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
MY + ++D VR+PP D ++ +L E +EG+ D++ G+I+ I ++E+G+G I+
Sbjct: 1 MYARIKLRDTVRVPPSKLGEDIEKVINSLLWEQFEGRLDREYGMIIGIESIEEIGEGRII 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
GDG Y +VVF+ + + P QEVVEG V++ V FGAFV IGP D L+H+SQ+ DDY+V+
Sbjct: 61 EGDGGVYFDVVFNAICFRPLHQEVVEGEVIEIVEFGAFVSIGPFDALLHMSQVTDDYMVF 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
DE+NK+ VG+E K +L+ GD VRARI+++S K R +++IGLTMRQP LG +WIE+E
Sbjct: 121 DEKNKRLVGRETKKVLQEGDKVRARIVSLSLKEREPEKSKIGLTMRQPWLGALKWIEEEI 180
Query: 181 RK 182
K
Sbjct: 181 EK 182
>gb|AAG20209.1| (AE005097) DNA-directed RNA polymerase subunit E'; RpoE'
[Halobacterium sp. NRC-1]
Length = 190
Score = 174 bits (437), Expect = 6e-43
Identities = 78/182 (42%), Positives = 121/182 (65%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
MYK +KD + +PP + +L++ EG+ D+D G I+++ EV ++G+G ++
Sbjct: 1 MYKRARLKDTIEVPPEYLGAVGPDLVKRLLQDKLEGRMDEDVGSIVTVTEVHDLGEGAVL 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
P Y+E FD + +EP+ QEVV+G VV+ V FGAFV IGP+DGL+H+SQ+ D+Y+ +
Sbjct: 61 PNRPGVYYEAEFDAVTFEPEMQEVVDGEVVEVVSFGAFVGIGPVDGLLHVSQISDEYLAF 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
DE N+Q +E +L GD+VRARI+T S R RE++IGLT +QPGLGK W++ ++
Sbjct: 121 DEENQQLASRESNQVLGTGDSVRARIVTKSIDERNPRESKIGLTAKQPGLGKHGWLQADR 180
Query: 181 RK 182
+
Sbjct: 181 EQ 182
>gb|AAK40742.1| DNA-directed RNA polymerase, subunit E' (rpoE1) [Sulfolobus
solfataricus]
Length = 180
Score = 168 bits (422), Expect = 3e-41
Identities = 89/181 (49%), Positives = 120/181 (66%), Gaps = 1/181 (0%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
MYK++ + +VRIPP F E A+ LR+ Y+ K KD G++L+I+ VK +GI+V
Sbjct: 1 MYKLIKARSIVRIPPNEFGKPLNEIALNELRQQYQEKILKDLGLVLAILNVKTSEEGILV 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
GDGATYHEV FD++ + P QEVVEG V+ +G FV +GPMDGLVHISQ+ DD + Y
Sbjct: 61 FGDGATYHEVEFDMITYVPVVQEVVEGEVLQVDNYGIFVNLGPMDGLVHISQITDDTLKY 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
D G++ K +++ GD VRAR+I+V A + R RI LTMRQP LGK EWI + K
Sbjct: 121 DNVRGIIFGEKSKKVIQKGDKVRARVISV-ASTVTGRLPRIALTMRQPYLGKLEWITQTK 179
Query: 181 R 181
+
Sbjct: 180 K 180
>sp|P39466|RPOE_SULAC DNA-DIRECTED RNA POLYMERASE SUBUNIT E >gi|481466|pir||S38658
DNA-directed RNA polymerase (EC 2.7.7.6) chain E
[validated] - Sulfolobus acidocaldarius
>gi|415999|emb|CAA53164.1| (X75411) DNA-directed RNA
polymerase [Sulfolobus acidocaldarius]
Length = 248
Score = 155 bits (387), Expect = 4e-37
Identities = 85/188 (45%), Positives = 115/188 (60%), Gaps = 5/188 (2%)
Query: 1 MYKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIV 60
M+K+V K +VRIPP F E A+ +LR+ Y+ K KD GV+L IV K +G I+
Sbjct: 1 MFKLVRAKGIVRIPPEYFGQSVDEIAIKILRQEYQEKLIKDIGVVLGIVNAKASEEGFII 60
Query: 61 PGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVVY 120
GDGATYHEV FD+LV+ P EV+EG V +G +V +GP+DGLVHISQ+ DD + +
Sbjct: 61 FGDGATYHEVEFDMLVYTPIIHEVIEGEVSQVDNYGVYVNMGPVDGLVHISQITDDNLKF 120
Query: 121 DERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKEK 180
D +G++ K + GD VRA II+ S S R RI LTM+QP LGK +K
Sbjct: 121 DSNRGILIGEKSKKSITKGDRVRAMIISASMSSG--RLPRIALTMKQPYLGK---NRMDK 175
Query: 181 RKEKEGKK 188
+ +G+K
Sbjct: 176 SRNSKGEK 183
>emb|CAC12217.1| (AL445066) DNA-directed RNA polymerase, subunit E related protein
[Thermoplasma acidophilum]
Length = 189
Score = 152 bits (379), Expect = 4e-36
Identities = 77/186 (41%), Positives = 115/186 (61%), Gaps = 10/186 (5%)
Query: 4 IVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEG--------KYDKDEG--VILSIVEVKE 53
+V K + R+PP M D A V + + EG K ++EG +++S++ +
Sbjct: 1 MVEDKYIARVPPEMLGEDYDVAVREVTKRSLEGRLVDVINEKTSQNEGKYLVISVLYTES 60
Query: 54 VGDGIIVPGDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQL 113
+G+G IV GDG Y EV + LV+ P+ QE+V+G VV FGAF++ +GL+HISQ+
Sbjct: 61 IGEGTIVHGDGGVYQEVKYRALVYYPEMQEIVDGEVVSVQKFGAFIKFSSFEGLLHISQI 120
Query: 114 MDDYVVYDERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKF 173
MDD + D NK+F+GKE K LK+GD VR RI+ ++ S I +++IG TM+Q GLGK
Sbjct: 121 MDDRIDIDLENKRFIGKETKRDLKVGDRVRVRIVAMNLASSSISDSKIGFTMKQVGLGKL 180
Query: 174 EWIEKE 179
+W+ KE
Sbjct: 181 DWLAKE 186
>pir||A72784 probable DNA-directed RNA polymerase subunit E' APE0257 - Aeropyrum
pernix (strain K1) >gi|5103650|dbj|BAA79171.1|
(AP000058) 186aa long hypothetical DNA-directed RNA
polymerase subunit E' [Aeropyrum pernix]
Length = 186
Score = 132 bits (330), Expect = 2e-30
Identities = 74/183 (40%), Positives = 109/183 (59%), Gaps = 2/183 (1%)
Query: 2 YKIVTVKDVVRIPPRMFTMDPKEAAMLVLRETYEGKYDKDEGVILSIVEVKEVGDGIIVP 61
Y+ V + + P +++ + + A L EG+ D++ GVI+++++ K VGDGII P
Sbjct: 3 YEYVVEEWIGMEPASIYSSEDLDKAALEHLRRLEGRIDEEMGVIVAVLDAKIVGDGIIPP 62
Query: 62 --GDGATYHEVVFDVLVWEPKQQEVVEGTVVDAVPFGAFVRIGPMDGLVHISQLMDDYVV 119
GD + Y+ V + VL ++P Q E+V G V A FG V +GP+DG VH +Q+MD+ V
Sbjct: 63 VSGDPSIYYPVRYRVLAFKPVQLEIVRGVVTRADDFGLVVNLGPLDGKVHRNQIMDEGVE 122
Query: 120 YDERNKQFVGKEKKYLLKIGDAVRARIITVSAKSRIIRENRIGLTMRQPGLGKFEWIEKE 179
FVG K +++GD VRAR++ VS SR + IGLTMRQP LGK EW+ E
Sbjct: 123 LLPDRSGFVGLASKREIRVGDVVRARVVHVSRPSRTAQIIGIGLTMRQPYLGKEEWVAPE 182
Query: 180 KRK 182
+K
Sbjct: 183 AKK 185
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.141 0.402
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70868835
Number of Sequences: 2977
Number of extensions: 2899064
Number of successful extensions: 6851
Number of sequences better than 1.0e-10: 10
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 6836
Number of HSP's gapped (non-prelim): 10
length of query: 188
length of database: 189,106,746
effective HSP length: 54
effective length of query: 134
effective length of database: 156,788,448
effective search space: 21009652032
effective search space used: 21009652032
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 162 (67.5 bits)