BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1151 (act-1) DE:PYRUVATE FORMATE-LYASE ACTIVATING ENZYME
(ACT-1.
(302 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75027 pyruvate formate-lyase activating enzyme (act-1) PAB... 616 e-176
pir||F71212 hypothetical protein PH1966 - Pyrococcus horikoshii ... 582 e-165
gi|11498523 pyruvate formate-lyase activating enzyme (act-2) [Ar... 257 1e-67
sp|Q58214|Y804_METJA HYPOTHETICAL PROTEIN MJ0804 >gi|2826326|gb|... 172 5e-42
pir||D64400 hypothetical protein MJ0804 - Methanococcus jannaschii 171 1e-41
>pir||E75027 pyruvate formate-lyase activating enzyme (act-1) PAB1151 -
Pyrococcus abyssi (strain Orsay)
>gi|5459173|emb|CAB50659.1| (AJ248288) PYRUVATE
FORMATE-LYASE ACTIVATING ENZYME ( ACT-1. [Pyrococcus
abyssi]
Length = 302
Score = 616 bits (1572), Expect = e-176
Identities = 302/302 (100%), Positives = 302/302 (100%)
Query: 1 MIKYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSY 60
MIKYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSY
Sbjct: 1 MIKYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSY 60
Query: 61 ELTPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLR 120
ELTPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLR
Sbjct: 61 ELTPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLR 120
Query: 121 KRFPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKI 180
KRFPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKI
Sbjct: 121 KRFPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKI 180
Query: 181 VGEDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLD 240
VGEDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLD
Sbjct: 181 VGEDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLD 240
Query: 241 FERAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKI 300
FERAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKI
Sbjct: 241 FERAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKI 300
Query: 301 EL 302
EL
Sbjct: 301 EL 302
>pir||F71212 hypothetical protein PH1966 - Pyrococcus horikoshii
>gi|3258410|dbj|BAA31093.1| (AP000007) 303aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 303
Score = 582 bits (1485), Expect = e-165
Identities = 277/300 (92%), Positives = 294/300 (97%)
Query: 3 KYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYEL 62
+YSWE+ AR MG+EPQRLENKEARELKKFV+E+TWPTHCNYCQGLDL NPNPVHHPSYEL
Sbjct: 4 RYSWEELARAMGIEPQRLENKEARELKKFVDEMTWPTHCNYCQGLDLTNPNPVHHPSYEL 63
Query: 63 TPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLRKR 122
TPACNHDCIFCYSNVAVKLGKAPKPGYYGW+NPK ITISQYGEPLLSPRIVEVNKMLRKR
Sbjct: 64 TPACNHDCIFCYSNVAVKLGKAPKPGYYGWDNPKVITISQYGEPLLSPRIVEVNKMLRKR 123
Query: 123 FPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVG 182
FPEARLDLQTNGSLLT+ELWEKLDFDLVMISLNAADREKH+RIANADTF+ VVNALKIVG
Sbjct: 124 FPEARLDLQTNGSLLTRELWEKLDFDLVMISLNAADREKHRRIANADTFEQVVNALKIVG 183
Query: 183 EDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFE 242
EDKSVRSVVRT+FMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERL+KAGLDFE
Sbjct: 184 EDKSVRSVVRTVFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLKKAGLDFE 243
Query: 243 RAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKIEL 302
RAESIRE LKAAMEAKKYIDVRISGC+LVQL+QMDPITLYSV+RVAREVVPLVKRSK+++
Sbjct: 244 RAESIRELLKAAMEAKKYIDVRISGCILVQLKQMDPITLYSVRRVAREVVPLVKRSKLDI 303
>gi|11498523 pyruvate formate-lyase activating enzyme (act-2) [Archaeoglobus
fulgidus] >gi|7450322|pir||F69364 pyruvate formate-lyase
activating enzyme (act-2) homolog - Archaeoglobus
fulgidus >gi|2649678|gb|AAB90319.1| (AE001040) pyruvate
formate-lyase activating enzyme (act-2) [Archaeoglobus
fulgidus]
Length = 283
Score = 257 bits (650), Expect = 1e-67
Identities = 139/277 (50%), Positives = 188/277 (67%), Gaps = 13/277 (4%)
Query: 27 ELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYELTPACNHDCIFCYSNVAVKLGKAPK 86
E+KKF+E + +P HC +C+G D N NPVHHPSYE+TP+CN CIFCYS VA G APK
Sbjct: 7 EIKKFLESLPYPKHCIHCEGTDENAENPVHHPSYEVTPSCNLRCIFCYSRVAQLKGTAPK 66
Query: 87 PGYYGWENPKAITISQYGEPLL--SPRIVEVNKMLRKRFPEARLDLQTNGSLLTKELWEK 144
PGYYG NPKAITISQYGEP + + R+VE+ + LR+RF E R+D+QTNG+LL E+
Sbjct: 67 PGYYGSLNPKAITISQYGEPFVVGTSRVVEIIRKLRERFGEVRIDIQTNGTLLEPEILGS 126
Query: 145 LDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVGEDKSVRSVVRTIFMPGINDEDI 204
+ D+VMISL+A RE + I D F+ VV ++ + E +VVRT+FMPGINDE++
Sbjct: 127 -EADIVMISLDAGSRESYAEITGKDFFERVVKNIEKMSE--HTYTVVRTVFMPGINDEEL 183
Query: 205 PKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFERAESIREFLKAAMEAKKYIDVR 264
+IAE+A+ DE+ LQPL+I+ N E L++ LD ERAES+ ++L+ A + VR
Sbjct: 184 ERIAEIASQ--ADELFLQPLSIYRENTELLKR--LDVERAESLGDYLQTAYRLSEIASVR 239
Query: 265 ISGCLLVQ----LRQMDPITLYSVKRVAREVVPLVKR 297
I GCLL+ LR+ + L VKR A VP+++R
Sbjct: 240 IPGCLLLNMRWFLREFEIGELMLVKRDAFGSVPMMQR 276
>sp|Q58214|Y804_METJA HYPOTHETICAL PROTEIN MJ0804 >gi|2826326|gb|AAB98804.1| (U67525)
pyruvate formate-lyase activating enzyme (act)
[Methanococcus jannaschii]
Length = 286
Score = 172 bits (431), Expect = 5e-42
Identities = 109/289 (37%), Positives = 164/289 (56%), Gaps = 24/289 (8%)
Query: 20 LENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYELTPACNHDCIFCYSNVAV 79
L N+ +L+ + + + HC C+G++L NP HHPS ELT CN +CI+CYS +
Sbjct: 4 LRNEICDKLENIAKILKFVRHCIGCEGINLEIENPQHHPSIELTQKCNLNCIYCYSRL-- 61
Query: 80 KLGKAPKPGYYG-WENPKAITISQYGEPLLS----PRIVEVNKMLRKRFPEARLDLQTNG 134
K K G YG E + +TISQYGEPLL + +E K L R+DLQTNG
Sbjct: 62 ---KTVKRGIYGNLEEAETVTISQYGEPLLDLEGVKKAIEFCKDL-----GLRVDLQTNG 113
Query: 135 SLLTKELWEK---LDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVGEDKSVRSVV 191
+LL +E+ ++ L DL+MISL++ REK+K + D F+ V+N +KI K + ++V
Sbjct: 114 TLLNEEIIKELKDLGLDLIMISLSSFSREKYKLLTGKDYFNRVLNNIKIA--SKYLHTIV 171
Query: 192 RTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFERAESIREFL 251
R+I++PG ND ++ +A+ + DE+ + L ++ N L+ AG+D IR+ L
Sbjct: 172 RSIYIPGFNDNELLNLAKELNNYA-DEIMVHQLISYKENENLLKNAGIDLNNLGRIRDLL 230
Query: 252 KAAMEAKK---YIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKR 297
E +K I+V I GCLLVQL++MD L ++ VP +KR
Sbjct: 231 LIVDEMQKNAPKINVTIKGCLLVQLKEMDGFILNNITYDVFSEVPDIKR 279
>pir||D64400 hypothetical protein MJ0804 - Methanococcus jannaschii
Length = 286
Score = 171 bits (428), Expect = 1e-41
Identities = 109/289 (37%), Positives = 163/289 (55%), Gaps = 24/289 (8%)
Query: 20 LENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYELTPACNHDCIFCYSNVAV 79
L N+ +L+ + + HC C+G++L NP HHPS ELT CN +CI+CYS +
Sbjct: 4 LRNEICDKLENIAKIXKFVRHCIGCEGINLEIENPQHHPSIELTQKCNLNCIYCYSRL-- 61
Query: 80 KLGKAPKPGYYG-WENPKAITISQYGEPLLS----PRIVEVNKMLRKRFPEARLDLQTNG 134
K K G YG E + +TISQYGEPLL + +E K L R+DLQTNG
Sbjct: 62 ---KTVKRGIYGNLEEAETVTISQYGEPLLDLEGVKKAIEFCKDL-----GLRVDLQTNG 113
Query: 135 SLLTKELWEK---LDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVGEDKSVRSVV 191
+LL +E+ ++ L DL+MISL++ REK+K + D F+ V+N +KI K + ++V
Sbjct: 114 TLLNEEIIKELKDLGLDLIMISLSSFSREKYKLLTGKDYFNRVLNNIKIA--SKYLHTIV 171
Query: 192 RTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFERAESIREFL 251
R+I++PG ND ++ +A+ + DE+ + L ++ N L+ AG+D IR+ L
Sbjct: 172 RSIYIPGFNDNELLNLAKELNNYA-DEIMVHQLISYKENENLLKNAGIDLNNLGRIRDLL 230
Query: 252 KAAMEAKK---YIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKR 297
E +K I+V I GCLLVQL++MD L ++ VP +KR
Sbjct: 231 LIVDEMQKNAPKINVTIKGCLLVQLKEMDGFILNNITYDVFSEVPDIKR 279
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.137 0.405
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109903702
Number of Sequences: 2977
Number of extensions: 4535006
Number of successful extensions: 10721
Number of sequences better than 1.0e-10: 5
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 10701
Number of HSP's gapped (non-prelim): 5
length of query: 302
length of database: 189,106,746
effective HSP length: 55
effective length of query: 247
effective length of database: 156,189,961
effective search space: 38578920367
effective search space used: 38578920367
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 165 (68.7 bits)