BLASTP 2.0.10 [Aug-26-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= PAB1151 (act-1) DE:PYRUVATE FORMATE-LYASE ACTIVATING ENZYME
(ACT-1.
         (302 letters)

Database: ./suso.pep; /banques/blast2/nr.pep
           598,487 sequences; 189,106,746 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||E75027 pyruvate formate-lyase activating enzyme (act-1) PAB...   616  e-176
pir||F71212 hypothetical protein PH1966 - Pyrococcus horikoshii ...   582  e-165
gi|11498523 pyruvate formate-lyase activating enzyme (act-2) [Ar...   257  1e-67
sp|Q58214|Y804_METJA HYPOTHETICAL PROTEIN MJ0804 >gi|2826326|gb|...   172  5e-42
pir||D64400 hypothetical protein MJ0804 - Methanococcus jannaschii    171  1e-41

>pir||E75027 pyruvate formate-lyase activating enzyme (act-1) PAB1151 -
           Pyrococcus abyssi (strain Orsay)
           >gi|5459173|emb|CAB50659.1| (AJ248288) PYRUVATE
           FORMATE-LYASE ACTIVATING ENZYME ( ACT-1. [Pyrococcus
           abyssi]
           Length = 302
           
 Score =  616 bits (1572), Expect = e-176
 Identities = 302/302 (100%), Positives = 302/302 (100%)

Query: 1   MIKYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSY 60
           MIKYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSY
Sbjct: 1   MIKYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSY 60

Query: 61  ELTPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLR 120
           ELTPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLR
Sbjct: 61  ELTPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLR 120

Query: 121 KRFPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKI 180
           KRFPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKI
Sbjct: 121 KRFPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKI 180

Query: 181 VGEDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLD 240
           VGEDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLD
Sbjct: 181 VGEDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLD 240

Query: 241 FERAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKI 300
           FERAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKI
Sbjct: 241 FERAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKI 300

Query: 301 EL 302
           EL
Sbjct: 301 EL 302


>pir||F71212 hypothetical protein PH1966 - Pyrococcus horikoshii
           >gi|3258410|dbj|BAA31093.1| (AP000007) 303aa long
           hypothetical protein [Pyrococcus horikoshii]
           Length = 303
           
 Score =  582 bits (1485), Expect = e-165
 Identities = 277/300 (92%), Positives = 294/300 (97%)

Query: 3   KYSWEDFARLMGVEPQRLENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYEL 62
           +YSWE+ AR MG+EPQRLENKEARELKKFV+E+TWPTHCNYCQGLDL NPNPVHHPSYEL
Sbjct: 4   RYSWEELARAMGIEPQRLENKEARELKKFVDEMTWPTHCNYCQGLDLTNPNPVHHPSYEL 63

Query: 63  TPACNHDCIFCYSNVAVKLGKAPKPGYYGWENPKAITISQYGEPLLSPRIVEVNKMLRKR 122
           TPACNHDCIFCYSNVAVKLGKAPKPGYYGW+NPK ITISQYGEPLLSPRIVEVNKMLRKR
Sbjct: 64  TPACNHDCIFCYSNVAVKLGKAPKPGYYGWDNPKVITISQYGEPLLSPRIVEVNKMLRKR 123

Query: 123 FPEARLDLQTNGSLLTKELWEKLDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVG 182
           FPEARLDLQTNGSLLT+ELWEKLDFDLVMISLNAADREKH+RIANADTF+ VVNALKIVG
Sbjct: 124 FPEARLDLQTNGSLLTRELWEKLDFDLVMISLNAADREKHRRIANADTFEQVVNALKIVG 183

Query: 183 EDKSVRSVVRTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFE 242
           EDKSVRSVVRT+FMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERL+KAGLDFE
Sbjct: 184 EDKSVRSVVRTVFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLKKAGLDFE 243

Query: 243 RAESIREFLKAAMEAKKYIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKRSKIEL 302
           RAESIRE LKAAMEAKKYIDVRISGC+LVQL+QMDPITLYSV+RVAREVVPLVKRSK+++
Sbjct: 244 RAESIRELLKAAMEAKKYIDVRISGCILVQLKQMDPITLYSVRRVAREVVPLVKRSKLDI 303


>gi|11498523 pyruvate formate-lyase activating enzyme (act-2) [Archaeoglobus
           fulgidus] >gi|7450322|pir||F69364 pyruvate formate-lyase
           activating enzyme (act-2) homolog - Archaeoglobus
           fulgidus >gi|2649678|gb|AAB90319.1| (AE001040) pyruvate
           formate-lyase activating enzyme (act-2) [Archaeoglobus
           fulgidus]
           Length = 283
           
 Score =  257 bits (650), Expect = 1e-67
 Identities = 139/277 (50%), Positives = 188/277 (67%), Gaps = 13/277 (4%)

Query: 27  ELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYELTPACNHDCIFCYSNVAVKLGKAPK 86
           E+KKF+E + +P HC +C+G D N  NPVHHPSYE+TP+CN  CIFCYS VA   G APK
Sbjct: 7   EIKKFLESLPYPKHCIHCEGTDENAENPVHHPSYEVTPSCNLRCIFCYSRVAQLKGTAPK 66

Query: 87  PGYYGWENPKAITISQYGEPLL--SPRIVEVNKMLRKRFPEARLDLQTNGSLLTKELWEK 144
           PGYYG  NPKAITISQYGEP +  + R+VE+ + LR+RF E R+D+QTNG+LL  E+   
Sbjct: 67  PGYYGSLNPKAITISQYGEPFVVGTSRVVEIIRKLRERFGEVRIDIQTNGTLLEPEILGS 126

Query: 145 LDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVGEDKSVRSVVRTIFMPGINDEDI 204
            + D+VMISL+A  RE +  I   D F+ VV  ++ + E     +VVRT+FMPGINDE++
Sbjct: 127 -EADIVMISLDAGSRESYAEITGKDFFERVVKNIEKMSE--HTYTVVRTVFMPGINDEEL 183

Query: 205 PKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFERAESIREFLKAAMEAKKYIDVR 264
            +IAE+A+    DE+ LQPL+I+  N E L++  LD ERAES+ ++L+ A    +   VR
Sbjct: 184 ERIAEIASQ--ADELFLQPLSIYRENTELLKR--LDVERAESLGDYLQTAYRLSEIASVR 239

Query: 265 ISGCLLVQ----LRQMDPITLYSVKRVAREVVPLVKR 297
           I GCLL+     LR+ +   L  VKR A   VP+++R
Sbjct: 240 IPGCLLLNMRWFLREFEIGELMLVKRDAFGSVPMMQR 276


>sp|Q58214|Y804_METJA HYPOTHETICAL PROTEIN MJ0804 >gi|2826326|gb|AAB98804.1| (U67525)
           pyruvate formate-lyase activating enzyme (act)
           [Methanococcus jannaschii]
           Length = 286
           
 Score =  172 bits (431), Expect = 5e-42
 Identities = 109/289 (37%), Positives = 164/289 (56%), Gaps = 24/289 (8%)

Query: 20  LENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYELTPACNHDCIFCYSNVAV 79
           L N+   +L+   + + +  HC  C+G++L   NP HHPS ELT  CN +CI+CYS +  
Sbjct: 4   LRNEICDKLENIAKILKFVRHCIGCEGINLEIENPQHHPSIELTQKCNLNCIYCYSRL-- 61

Query: 80  KLGKAPKPGYYG-WENPKAITISQYGEPLLS----PRIVEVNKMLRKRFPEARLDLQTNG 134
              K  K G YG  E  + +TISQYGEPLL      + +E  K L       R+DLQTNG
Sbjct: 62  ---KTVKRGIYGNLEEAETVTISQYGEPLLDLEGVKKAIEFCKDL-----GLRVDLQTNG 113

Query: 135 SLLTKELWEK---LDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVGEDKSVRSVV 191
           +LL +E+ ++   L  DL+MISL++  REK+K +   D F+ V+N +KI    K + ++V
Sbjct: 114 TLLNEEIIKELKDLGLDLIMISLSSFSREKYKLLTGKDYFNRVLNNIKIA--SKYLHTIV 171

Query: 192 RTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFERAESIREFL 251
           R+I++PG ND ++  +A+   +   DE+ +  L  ++ N   L+ AG+D      IR+ L
Sbjct: 172 RSIYIPGFNDNELLNLAKELNNYA-DEIMVHQLISYKENENLLKNAGIDLNNLGRIRDLL 230

Query: 252 KAAMEAKK---YIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKR 297
               E +K    I+V I GCLLVQL++MD   L ++       VP +KR
Sbjct: 231 LIVDEMQKNAPKINVTIKGCLLVQLKEMDGFILNNITYDVFSEVPDIKR 279


>pir||D64400 hypothetical protein MJ0804 - Methanococcus jannaschii
           Length = 286
           
 Score =  171 bits (428), Expect = 1e-41
 Identities = 109/289 (37%), Positives = 163/289 (55%), Gaps = 24/289 (8%)

Query: 20  LENKEARELKKFVEEITWPTHCNYCQGLDLNNPNPVHHPSYELTPACNHDCIFCYSNVAV 79
           L N+   +L+   +   +  HC  C+G++L   NP HHPS ELT  CN +CI+CYS +  
Sbjct: 4   LRNEICDKLENIAKIXKFVRHCIGCEGINLEIENPQHHPSIELTQKCNLNCIYCYSRL-- 61

Query: 80  KLGKAPKPGYYG-WENPKAITISQYGEPLLS----PRIVEVNKMLRKRFPEARLDLQTNG 134
              K  K G YG  E  + +TISQYGEPLL      + +E  K L       R+DLQTNG
Sbjct: 62  ---KTVKRGIYGNLEEAETVTISQYGEPLLDLEGVKKAIEFCKDL-----GLRVDLQTNG 113

Query: 135 SLLTKELWEK---LDFDLVMISLNAADREKHKRIANADTFDAVVNALKIVGEDKSVRSVV 191
           +LL +E+ ++   L  DL+MISL++  REK+K +   D F+ V+N +KI    K + ++V
Sbjct: 114 TLLNEEIIKELKDLGLDLIMISLSSFSREKYKLLTGKDYFNRVLNNIKIA--SKYLHTIV 171

Query: 192 RTIFMPGINDEDIPKIAELAASLGIDEMHLQPLTIHELNVERLRKAGLDFERAESIREFL 251
           R+I++PG ND ++  +A+   +   DE+ +  L  ++ N   L+ AG+D      IR+ L
Sbjct: 172 RSIYIPGFNDNELLNLAKELNNYA-DEIMVHQLISYKENENLLKNAGIDLNNLGRIRDLL 230

Query: 252 KAAMEAKK---YIDVRISGCLLVQLRQMDPITLYSVKRVAREVVPLVKR 297
               E +K    I+V I GCLLVQL++MD   L ++       VP +KR
Sbjct: 231 LIVDEMQKNAPKINVTIKGCLLVQLKEMDGFILNNITYDVFSEVPDIKR 279


  Database: ./suso.pep
    Posted date:  Jul 6, 2001  5:57 PM
  Number of letters in database: 840,471
  Number of sequences in database:  2977
  
  Database: /banques/blast2/nr.pep
    Posted date:  Dec 14, 2000 12:46 PM
  Number of letters in database: 188,266,275
  Number of sequences in database:  595,510
  
Lambda     K      H
   0.320    0.137    0.405 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109903702
Number of Sequences: 2977
Number of extensions: 4535006
Number of successful extensions: 10721
Number of sequences better than 1.0e-10: 5
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 10701
Number of HSP's gapped (non-prelim): 5
length of query: 302
length of database: 189,106,746
effective HSP length: 55
effective length of query: 247
effective length of database: 156,189,961
effective search space: 38578920367
effective search space used: 38578920367
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 165 (68.7 bits)