BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1213 (hydG-like) DE:HYDROGENASE, GAMMA CHAIN (HYDG).
(292 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75024 hydrogenase, gamma chain (hydg). PAB1213 - Pyrococcu... 604 e-172
pir||E71200 hypothetical protein PH1874 - Pyrococcus horikoshii ... 541 e-153
pir||F75069 hydrogenase, chain gamma related protein PAB1737 - P... 241 7e-63
sp|Q9X1X4|PYRK_THEMA PROBABLE DIHYDROOROTATE DEHYDROGENASE ELECT... 215 5e-55
pir||F71076 hypothetical protein PH0878 - Pyrococcus horikoshii ... 183 2e-45
pir||E71288 probable hydrogenase, gamma chain (hydG) - syphilis ... 169 4e-41
pir||G70479 hypothetical protein aq_2098 - Aquifex aeolicus >gi|... 126 3e-28
gb|AAB93308.1| (L04500) unknown [Eubacterium acidaminophilum] 94 1e-18
sp|Q58841|PYRK_METJA PROBABLE DIHYDROOROTATE DEHYDROGENASE ELECT... 71 1e-11
>pir||C75024 hydrogenase, gamma chain (hydg). PAB1213 - Pyrococcus abyssi
(strain Orsay) >gi|5459147|emb|CAB50633.1| (AJ248288)
HYDROGENASE, GAMMA CHAIN (HYDG). [Pyrococcus abyssi]
Length = 292
Score = 604 bits (1540), Expect = e-172
Identities = 292/292 (100%), Positives = 292/292 (100%)
Query: 1 MLIVYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEG 60
MLIVYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEG
Sbjct: 1 MLIVYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEG 60
Query: 61 WIVLIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPI 120
WIVLIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPI
Sbjct: 61 WIVLIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPI 120
Query: 121 AKAWQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALT 180
AKAWQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALT
Sbjct: 121 AKAWQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALT 180
Query: 181 GKVRELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTV 240
GKVRELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTV
Sbjct: 181 GKVRELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTV 240
Query: 241 GGEIKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYMEELKSKGVM 292
GGEIKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYMEELKSKGVM
Sbjct: 241 GGEIKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYMEELKSKGVM 292
>pir||E71200 hypothetical protein PH1874 - Pyrococcus horikoshii
>gi|3258313|dbj|BAA30996.1| (AP000007) 298aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 298
Score = 541 bits (1380), Expect = e-153
Identities = 254/292 (86%), Positives = 276/292 (93%)
Query: 1 MLIVYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEG 60
+ I+YKILEK+EIAMRNTWYK+ APHVAKKVQPGQFVIVRAF NGERIPLTP+MWDR+EG
Sbjct: 7 VFIMYKILEKKEIAMRNTWYKIYAPHVAKKVQPGQFVIVRAFPNGERIPLTPIMWDREEG 66
Query: 61 WIVLIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPI 120
WIVL+AFT+GKTTM+MA ELKEGD ILNVAGPLGNPAPMEKFGK+LAIGAY GI EVYPI
Sbjct: 67 WIVLVAFTKGKTTMKMAMELKEGDEILNVAGPLGNPAPMEKFGKILAIGAYVGIAEVYPI 126
Query: 121 AKAWQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALT 180
AKAWQEIGNDVTTLHVT EPMV+LK+YFEKAVSRHIVEPVK D +KSF EN KNVTKALT
Sbjct: 127 AKAWQEIGNDVTTLHVTFEPMVILKDYFEKAVSRHIVEPVKIDQEKSFQENTKNVTKALT 186
Query: 181 GKVRELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTV 240
KV+ELL+ E+WDLVFMVGPPGDQKAVF VVK++GIPM+VDLHPIMVDGTGMCGACRVTV
Sbjct: 187 AKVKELLDNENWDLVFMVGPPGDQKAVFEVVKKYGIPMKVDLHPIMVDGTGMCGACRVTV 246
Query: 241 GGEIKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYMEELKSKGVM 292
GGE+KFACVDGPEFDAY VDWDELIHRVGFYSKLEKLALEKYMEELK+KGV+
Sbjct: 247 GGEVKFACVDGPEFDAYLVDWDELIHRVGFYSKLEKLALEKYMEELKAKGVI 298
>pir||F75069 hydrogenase, chain gamma related protein PAB1737 - Pyrococcus
abyssi (strain Orsay) >gi|5458371|emb|CAB49859.1|
(AJ248286) hydrogenase, subunit gamma related protein
[Pyrococcus abyssi]
Length = 278
Score = 241 bits (609), Expect = 7e-63
Identities = 133/283 (46%), Positives = 180/283 (62%), Gaps = 10/283 (3%)
Query: 4 VYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEGWIV 63
++KIL K +A ++V+AP +AK +PGQFVI+R + GERIPLT +DRD+G I
Sbjct: 1 MFKILHKERLAPGINLFEVEAPRIAKHAKPGQFVIIRLHERGERIPLTIADFDRDKGSIT 60
Query: 64 LIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPIAKA 123
++A GKTT + T +EGDYI ++ GPLGNP+ ++KFG V+ +G G+ E+YP+A+A
Sbjct: 61 IVAQEVGKTTKELGT-YEEGDYIHDILGPLGNPSHVDKFGTVVMVGGGVGVAEIYPVARA 119
Query: 124 WQEIGNDVTT-LHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALTGK 182
+E GN V + L + +V ++ K IV D S+ MK T K
Sbjct: 120 MKEKGNYVISILGFRTKDLVFWEDKLRKVSDEVIVTT----NDGSY--GMKGFTTHALQK 173
Query: 183 VRELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTVGG 242
+ + E DLV VGP KAV + K +GI L+PIMVDGTGMCGACRVTVGG
Sbjct: 174 LID--EGRKIDLVHAVGPAIMMKAVAELTKPYGIKTVASLNPIMVDGTGMCGACRVTVGG 231
Query: 243 EIKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYMEE 285
EIKFACVDGPEFDA+ VDWDEL+ R+ +Y LE+++LEK+ E
Sbjct: 232 EIKFACVDGPEFDAHLVDWDELMKRLSYYRDLERISLEKWERE 274
>sp|Q9X1X4|PYRK_THEMA PROBABLE DIHYDROOROTATE DEHYDROGENASE ELECTRON TRANSFER SUBUNIT
>gi|7451851|pir||G72230 hypothetical protein TM1639 -
Thermotoga maritima (strain MSB8)
>gi|4982212|gb|AAD36706.1|AE001806_16 (AE001806)
electron transfer protein, putative [Thermotoga
maritima]
Length = 282
Score = 215 bits (542), Expect = 5e-55
Identities = 120/281 (42%), Positives = 177/281 (62%), Gaps = 14/281 (4%)
Query: 6 KILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEGWIVLI 65
+I++K +IA + + +P V+K+ +PGQFV++R + GERIPLT +EG ++
Sbjct: 8 EIVKKVKIAEDVFDFWIHSPSVSKEARPGQFVVIRLHEKGERIPLTVADTKPEEGLFRMV 67
Query: 66 AFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPIAKAWQ 125
GKTT ++ + KEGD IL+V GPLGNP+ +E +G VL +G GI +YPIAKA +
Sbjct: 68 VKVVGKTTHELSLK-KEGDTILDVVGPLGNPSEIENYGNVLLVGGGVGIATLYPIAKALK 126
Query: 126 EIGNDVTTL--HVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALTGKV 183
E GN++TT+ T + ++M+ E+ E I + + D S MK V +T +
Sbjct: 127 EAGNNITTVLGARTKDYLIMVDEFKE------ISDVLLVTDDGS--AGMKGV---VTDAM 175
Query: 184 RELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTVGGE 243
+L +D+ + VGP K +EFG+P+ V L+PIMVDGTGMCGACRVTV G+
Sbjct: 176 DKLFRERKFDICWAVGPTIMMKFCTLKAREFGVPIWVSLNPIMVDGTGMCGACRVTVSGQ 235
Query: 244 IKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYME 284
IKFACVDGPEF +VDWDEL+ R+ Y + EK++ E++++
Sbjct: 236 IKFACVDGPEFRGEEVDWDELLKRLAQYREQEKISYERFLK 276
>pir||F71076 hypothetical protein PH0878 - Pyrococcus horikoshii
>gi|3257289|dbj|BAA29972.1| (AP000004) 285aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 285
Score = 183 bits (461), Expect = 2e-45
Identities = 99/289 (34%), Positives = 164/289 (56%), Gaps = 10/289 (3%)
Query: 5 YKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEGWIVL 64
YKI +KR+++ + + +V+APH+AK +PGQFVI + GER+P++ ++ + G I +
Sbjct: 6 YKITDKRDLSPIDFFVEVEAPHIAKAWKPGQFVIFILHERGERVPMS--VYKAENGRIGM 63
Query: 65 IAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVL-AIGAYTGIVEVYPIAKA 123
GKT++++ E GD ++ GPLG P ++ +G V+ A A G E Y KA
Sbjct: 64 FIRKLGKTSLQLYYEFNVGDEFYSMVGPLGKPIKVKYYGNVVFASDAVCGQAENYATLKA 123
Query: 124 WQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALTGKV 183
+E+GN ++ + E F + +PV + + + + V
Sbjct: 124 MKEVGNYTISIQTFENAENVYPEEF-------LAKPVADEHYLTTEDGSVGIKGHYLDIV 176
Query: 184 RELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTVGGE 243
+EL+E + D++F G G + + + +GIP + IMVDGTGMCG+CR+ GE
Sbjct: 177 KELIEKDKVDIIFAGGKLGSLAKLAELTRPYGIPTYATVRQIMVDGTGMCGSCRILYDGE 236
Query: 244 IKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYMEELKSKGVM 292
+KFAC DGP FDA++VDW++++ R + + E+LA E+Y+E L+ KGV+
Sbjct: 237 VKFACRDGPVFDAHKVDWEDVLRRANRFLEQERLAKERYLEYLRKKGVI 285
>pir||E71288 probable hydrogenase, gamma chain (hydG) - syphilis spirochete
>gi|3323041|gb|AAC65704.1| (AE001245) hydrogenase, gamma
chain (hydG) [Treponema pallidum]
Length = 280
Score = 169 bits (423), Expect = 4e-41
Identities = 107/274 (39%), Positives = 145/274 (52%), Gaps = 11/274 (4%)
Query: 4 VYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQN-GERIPLTPVMWDRDEGWI 62
++ ILEK++ + + + KV AP +AK GQFV+V+ ERIPLT DEGWI
Sbjct: 1 MFSILEKKQFSPQVFYLKVHAPDIAKNRAAGQFVLVQLDDEYAERIPLTIADAHADEGWI 60
Query: 63 VLIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPIAK 122
L+ T G TTMR+ E + GD I V GPLGNP +E G V + G +YPIA+
Sbjct: 61 ALVIQTVGATTMRLC-EKEVGDSISVVLGPLGNPTLIENVGTVACVAGGVGAAPLYPIAQ 119
Query: 123 AWQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALTGK 182
A + GN V + +++ FE+ + E V D S+ +T
Sbjct: 120 AHKRAGNHVIVILGARNRDLII---FEEEMRALADELVIVTDDGSYGRK-----GLVTEP 171
Query: 183 VRELLETEDWDL-VFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTVG 241
+REL V +GPP K + FGI V L+ IM+DGTGMCG CRV VG
Sbjct: 172 LRELCXRASCPQEVVAIGPPIMMKFCAETTRPFGIRTVVSLNTIMIDGTGMCGGCRVNVG 231
Query: 242 GEIKFACVDGPEFDAYQVDWDELIHRVGFYSKLE 275
EIKF CVDGPEFD +QVD++ ++ R+ + E
Sbjct: 232 SEIKFVCVDGPEFDGHQVDFESMMRRMRAFQARE 265
>pir||G70479 hypothetical protein aq_2098 - Aquifex aeolicus
>gi|2984312|gb|AAC07835.1| (AE000772) putative protein
[Aquifex aeolicus]
Length = 279
Score = 126 bits (314), Expect = 3e-28
Identities = 88/284 (30%), Positives = 142/284 (49%), Gaps = 12/284 (4%)
Query: 4 VYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEGWIV 63
+YK+L K + + +VQ AK V+PGQ+ +V+A + E IPL+ D E
Sbjct: 1 MYKVLRKERLTPKAVLLEVQDER-AKLVKPGQYALVQANEESEPIPLS--FLDVKESSYT 57
Query: 64 LIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPMEKFGKVLAIGAYTGIVEVYPIAKA 123
+ G+ T+ +A ++E I + P G+P P++ +GKVL I G+ + I +A
Sbjct: 58 FLVEVGGRATLEIAELVEEK--IQFIEAPCGSPFPVKNYGKVLLISKNWGVAPLINIGRA 115
Query: 124 WQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALTGKV 183
+E N++ +HV + + + K S E + F D S E N AL +
Sbjct: 116 LKEESNEIYFVHVAEDEE---RVFLTKEASEISEEFLLFTEDGSLGEE-GNSEWALGYFI 171
Query: 184 RELLETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTVGGE 243
+ DL+ G D + + +E IP ++ ++D G+C ACRV V G+
Sbjct: 172 ENFGKP---DLIVSAGSNLDSQMINRFAEERNIPHIAMVNAHILDANGLCLACRVLVEGQ 228
Query: 244 IKFACVDGPEFDAYQVDWDELIHRVGFYSKLEKLALEKYMEELK 287
K AC+DGP FDA +VDWD I + FY + E+ AL+ ++ EL+
Sbjct: 229 EKLACIDGPWFDASKVDWDYAIQKEAFYKEEEEKALKTFLRELQ 272
>gb|AAB93308.1| (L04500) unknown [Eubacterium acidaminophilum]
Length = 168
Score = 94.4 bits (231), Expect = 1e-18
Identities = 57/160 (35%), Positives = 85/160 (52%), Gaps = 8/160 (5%)
Query: 4 VYKILEKREIAMRNTWYKVQAPHVAKKVQPGQFVIVRAFQNGERIPLTPVMWDRDEGWIV 63
++KI+EK+ +A K+ AP VAK +PGQFVI+R + GERIPLT +D++ G +
Sbjct: 1 MFKIVEKKVLAPSIFLMKIDAPRVAKSAKPGQFVIIRMDEKGERIPLTICDYDKEAGTVT 60
Query: 64 LIAFTRGKTTMRMATELKEGDYILNVAGPLGNPAPME-------KFGKVLAIGAYTGIVE 116
++ + G +T RMA E EGDY + GPLG + K K+L + G
Sbjct: 61 IVVQSMGCSTRRMA-EFNEGDYFSDFVGPLGRESEFVHESIDALKNKKILFVAGGVGTAP 119
Query: 117 VYPIAKAWQEIGNDVTTLHVTLEPMVMLKEYFEKAVSRHI 156
VYP K E G D + +M+ E K+VS+++
Sbjct: 120 VYPQVKWLHENGIDADVIIGAKNKEMMIMEEELKSVSKNL 159
>sp|Q58841|PYRK_METJA PROBABLE DIHYDROOROTATE DEHYDROGENASE ELECTRON TRANSFER SUBUNIT
>gi|2127836|pir||E64480 cytochrome-c3 hydrogenase gamma
chain homolog - Methanococcus jannaschii
>gi|1592091|gb|AAB99455.1| (U67585) cytochrome-c3
hydrogenase, gamma chain [Methanococcus jannaschii]
Length = 257
Score = 71.4 bits (172), Expect = 1e-11
Identities = 62/187 (33%), Positives = 88/187 (46%), Gaps = 18/187 (9%)
Query: 70 GKTTMRMATELKEGDYILNVAGPLGNPAPMEKFG-KVLAIGAYTGIVEVYPIAKAWQEIG 128
G+ T +M ELKEGD I+ V GP G E G KVLA+ G + + + + G
Sbjct: 62 GEFTKKMH-ELKEGD-IIGVRGPYGTY--FEPIGDKVLAVAGGIGAAPIITAVEEFSKQG 117
Query: 129 NDVTT-LHVTLEPMVMLKEYFEKAVSRHIVEPVKFDPDKSFPENMKNVTKALTGKVRELL 187
++TT L + ++ + FEK I D SF K T T K++E+L
Sbjct: 118 IEITTILGARTKEELLFLDRFEKVSRLEICTD-----DGSF--GFKGFT---TEKMKEVL 167
Query: 188 ETEDWDLVFMVGPPGDQKAVFNVVKEFGIPMRVDLHPIMVDGTGMCGACRVTVGGEIKFA 247
+ E +DL+ GP K V + E+ IP++V + M G G+CG C V E
Sbjct: 168 KEEKFDLIITCGPEIMMKKVVEIANEYNIPVQVSMERYMKCGIGICGQC--CVDDEGLCV 225
Query: 248 CVDGPEF 254
C DGP F
Sbjct: 226 CKDGPVF 232
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.140 0.425
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 115354149
Number of Sequences: 2977
Number of extensions: 5094841
Number of successful extensions: 10964
Number of sequences better than 1.0e-10: 9
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 10944
Number of HSP's gapped (non-prelim): 9
length of query: 292
length of database: 189,106,746
effective HSP length: 52
effective length of query: 240
effective length of database: 157,985,422
effective search space: 37916501280
effective search space used: 37916501280
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 165 (68.7 bits)