BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1219 (PAB1219) DE:METAL DEPENDENT HYDROLASE
(270 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75023 metal dependent hydrolase PAB1219 - Pyrococcus abyss... 552 e-156
pir||A71210 hypothetical protein PH1945 - Pyrococcus horikoshii ... 510 e-143
pir||E64471 hypothetical protein MJ1374 - Methanococcus jannasch... 232 4e-60
pir||D69200 conserved hypothetical protein MTH751 - Methanobacte... 199 3e-50
pir||B70440 conserved hypothetical protein aq_1625 - Aquifex aeo... 104 1e-21
>pir||B75023 metal dependent hydrolase PAB1219 - Pyrococcus abyssi (strain
Orsay) >gi|5459138|emb|CAB50624.1| (AJ248288) METAL
DEPENDENT HYDROLASE [Pyrococcus abyssi]
Length = 270
Score = 552 bits (1408), Expect = e-156
Identities = 270/270 (100%), Positives = 270/270 (100%)
Query: 1 MIEITFLGAGGGRFVTITQVRSTGGFFIKASKNIFVDPGPGALVRMIRYKLDPRKIDVLF 60
MIEITFLGAGGGRFVTITQVRSTGGFFIKASKNIFVDPGPGALVRMIRYKLDPRKIDVLF
Sbjct: 1 MIEITFLGAGGGRFVTITQVRSTGGFFIKASKNIFVDPGPGALVRMIRYKLDPRKIDVLF 60
Query: 61 ISHRHTDHCNDAEVIVEGMTYGVTKKRGTLIGSRSVVYGDEDHTPALSKYHLEALEEVHA 120
ISHRHTDHCNDAEVIVEGMTYGVTKKRGTLIGSRSVVYGDEDHTPALSKYHLEALEEVHA
Sbjct: 61 ISHRHTDHCNDAEVIVEGMTYGVTKKRGTLIGSRSVVYGDEDHTPALSKYHLEALEEVHA 120
Query: 121 PNPGDRFRLGNEEMIITPSQHSDPTTIGFRLKTSLGDISYIADTEYFPELVSWHDGSRVL 180
PNPGDRFRLGNEEMIITPSQHSDPTTIGFRLKTSLGDISYIADTEYFPELVSWHDGSRVL
Sbjct: 121 PNPGDRFRLGNEEMIITPSQHSDPTTIGFRLKTSLGDISYIADTEYFPELVSWHDGSRVL 180
Query: 181 IASVTRPRDMKIPYHLSTDDIVYMLKAMKQRPEVLVMTHLGMKMHFAGPYKEAQYIQNVT 240
IASVTRPRDMKIPYHLSTDDIVYMLKAMKQRPEVLVMTHLGMKMHFAGPYKEAQYIQNVT
Sbjct: 181 IASVTRPRDMKIPYHLSTDDIVYMLKAMKQRPEVLVMTHLGMKMHFAGPYKEAQYIQNVT 240
Query: 241 GVKTYVAKEGFRVTIDKEIKVKTLRPARFV 270
GVKTYVAKEGFRVTIDKEIKVKTLRPARFV
Sbjct: 241 GVKTYVAKEGFRVTIDKEIKVKTLRPARFV 270
>pir||A71210 hypothetical protein PH1945 - Pyrococcus horikoshii
>gi|3258389|dbj|BAA31072.1| (AP000007) 284aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 284
Score = 510 bits (1298), Expect = e-143
Identities = 242/270 (89%), Positives = 258/270 (94%)
Query: 1 MIEITFLGAGGGRFVTITQVRSTGGFFIKASKNIFVDPGPGALVRMIRYKLDPRKIDVLF 60
MIE+TFLGAGGGRFVTITQVRSTGGFFIKASKNIFVDPGPGAL+RMIRYKLDPRKIDVLF
Sbjct: 15 MIEVTFLGAGGGRFVTITQVRSTGGFFIKASKNIFVDPGPGALIRMIRYKLDPRKIDVLF 74
Query: 61 ISHRHTDHCNDAEVIVEGMTYGVTKKRGTLIGSRSVVYGDEDHTPALSKYHLEALEEVHA 120
ISHRHTDHCNDAEVIVEGMTYGVTK+RG LIGSRSVV+GD+ HTPALSKYHLEALEEVHA
Sbjct: 75 ISHRHTDHCNDAEVIVEGMTYGVTKRRGILIGSRSVVHGDDAHTPALSKYHLEALEEVHA 134
Query: 121 PNPGDRFRLGNEEMIITPSQHSDPTTIGFRLKTSLGDISYIADTEYFPELVSWHDGSRVL 180
PNPGDRF+LGNE+M+ITPS HSDPTTIGFRLKT+ GDISYIADTEYF L WHDG+RVL
Sbjct: 135 PNPGDRFKLGNEDMVITPSVHSDPTTIGFRLKTNFGDISYIADTEYFEGLEEWHDGTRVL 194
Query: 181 IASVTRPRDMKIPYHLSTDDIVYMLKAMKQRPEVLVMTHLGMKMHFAGPYKEAQYIQNVT 240
IASVTRPRDMKIPYHLSTDD+VYMLK MK++PEVL+MTHLGMKMHFAGPYKEA+YIQNVT
Sbjct: 195 IASVTRPRDMKIPYHLSTDDVVYMLKKMKEKPEVLIMTHLGMKMHFAGPYKEAEYIQNVT 254
Query: 241 GVKTYVAKEGFRVTIDKEIKVKTLRPARFV 270
GVKTYVAKEGFR+TI KEI VKTLRP RFV
Sbjct: 255 GVKTYVAKEGFRITIGKEIHVKTLRPVRFV 284
>pir||E64471 hypothetical protein MJ1374 - Methanococcus jannaschii
>gi|1592019|gb|AAB99381.1| (U67577) conserved
hypothetical protein [Methanococcus jannaschii]
Length = 266
Score = 232 bits (585), Expect = 4e-60
Identities = 116/255 (45%), Positives = 167/255 (65%), Gaps = 3/255 (1%)
Query: 2 IEITFLGAGGGRFVTITQVRSTGGFFIKASK-NIFVDPGPGALVRMIRYKLDPRKIDVLF 60
+EI FLG GGGR+ TITQ ++TGGF I ++ + VDPGPGA+VR+ K+ P + + LF
Sbjct: 3 VEIIFLGCGGGRWATITQKKATGGFRIHTNELRMHVDPGPGAIVRLNELKISPWRTNALF 62
Query: 61 ISHRHTDHCNDAEVIVEGMTYGVTKKRGTLIGSRSVVYGDEDHTPALSKYHLEALEEVHA 120
ISH H DH D E+IVE +T G+TKKRG +GS SVV G ++ +SKYH LEEV
Sbjct: 63 ISHCHPDHYTDGEIIVEAITQGMTKKRGVFLGSLSVVEGFGEYEYVVSKYHQSKLEEVRV 122
Query: 121 PNPGDRFRLGNEEMIITPSQHSDPTTIGFRLKTSLGDISYIADTEYFPELVSWHDGSRVL 180
PGD L + + T ++H DP IGFRL T GDI Y +DTE+ P+L+ DG R+L
Sbjct: 123 LYPGDSAELYDTTIKATHTKHGDPFGIGFRLSTIYGDIGYTSDTEFIPQLIEDFDGVRIL 182
Query: 181 IASVTRPRDMKIPYHLSTDDIVYMLKAMKQRPEVLVMTHLGMKMHFAGPYKEAQYIQNVT 240
IA++ R ++ +I HL ++D + ++ +M ++PE+L+M H+G+KM P EA+YI T
Sbjct: 183 IANIVRKKNERIKGHLCSNDAIDLINSMNKKPELLIMNHMGVKM--TNPQIEAEYISQNT 240
Query: 241 GVKTYVAKEGFRVTI 255
G++ A+ G +V +
Sbjct: 241 GIEVIPARLGLKVEL 255
>pir||D69200 conserved hypothetical protein MTH751 - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621840|gb|AAB85254.1| (AE000854) conserved protein
[Methanobacterium thermoautotrophicum]
Length = 241
Score = 199 bits (501), Expect = 3e-50
Identities = 105/222 (47%), Positives = 143/222 (64%), Gaps = 5/222 (2%)
Query: 14 FVTITQVRSTGGFFIK--ASKNIFVDPGPGALVRMIRYKLDPRKIDVLFISHRHTDHCND 71
F TITQ R TGG I A NI +DPGPGALVR ++ DPRK+D + +SH HTDH D
Sbjct: 6 FATITQKRMTGGLRIDGIAGMNIHIDPGPGALVRSYQFDSDPRKLDAVMVSHSHTDHYTD 65
Query: 72 AEVIVEGMTYGVTKKRGTLIGSRSVVYGDEDHTPALSKYHLEALEEVHAPNPGDRFRLGN 131
AEV++E MT G+T++RGT++GS SV+ G D P +S+YH+ + + G+ +G+
Sbjct: 66 AEVLIEAMTRGMTRQRGTILGSLSVMEGYRDWGPCISEYHMRRSRCI-TLSAGETVDIGD 124
Query: 132 EEMIITPSQHSDPTTIGFRLKTSLGDISYIADTEYFPELVSWHDGSRVLIASVTRPRDMK 191
E+ T + H DPT +GFRL+T +SY +DTEYF L +H G+ VLI+SV RP D
Sbjct: 125 LEVTGTGTVHGDPTGVGFRLRTDDLSVSYTSDTEYFDGLADYHRGADVLISSVIRPGDEH 184
Query: 192 IPYHLSTDDIVYMLKAMKQRPEVLVMTHLGMKMHFAGPYKEA 233
I H+ TDD + +++ +K P + VMTHLGMKM P EA
Sbjct: 185 IRGHMCTDDFIKLVEDVK--PGIAVMTHLGMKMILNDPEMEA 224
>pir||B70440 conserved hypothetical protein aq_1625 - Aquifex aeolicus
>gi|2983972|gb|AAC07519.1| (AE000748) hypothetical
protein [Aquifex aeolicus]
Length = 249
Score = 104 bits (257), Expect = 1e-21
Identities = 72/256 (28%), Positives = 124/256 (48%), Gaps = 9/256 (3%)
Query: 2 IEITFLGAGGGRFVTITQVRSTGGFFIKASKN-IFVDPGPGALVRMIRYKLDPRKIDVLF 60
+++ FLG GGR VT +R +GGF ++ + + + VDPGPGA V + + +D +KID++
Sbjct: 1 MKVIFLGTAGGRVVTFNLIRRSGGFLLEENSSYLHVDPGPGAFVYLHQLGIDYKKIDLIV 60
Query: 61 ISHRHTDHCNDAEVIVEGMTYGVTKKRGTLIGSRSVVYGDEDHTPALSKYHLEALEEVHA 120
+SH H DH D ++E T G + +L +S + G + + Y+ L +
Sbjct: 61 LSHLHLDHSADVNTLIEAATNGGKYRNLSLFAPKSALEGAD---RVVFPYNRRRLVKEET 117
Query: 121 PNPGDRFRLGNEEM-IITPSQHSDPTTIGFRLKTSLGDISYIADTEYFPELVSWHDGSRV 179
+ G N ++ + +H T G L+ + +S E L + + +
Sbjct: 118 FSEGSEHHYKNWKVKAVIKHKHHRTETYGLFLQNKVVYVSCAGFEERM--LTLYPKNADL 175
Query: 180 LIASVTRPRDMKIPYHLSTDDIVYMLKAMKQRPEVLVMTHLGMKMHFAGPYKEAQYIQNV 239
+I + T R HLS +D ++ +K P+ V+TH M+M GP K A+ I
Sbjct: 176 MIINTTFYRKRPNVEHLSAEDAKVLISHLK--PKKAVITHYSMEMLQIGPDKVAEEITKE 233
Query: 240 TGVKTYVAKEGFRVTI 255
TG++T AK+ + I
Sbjct: 234 TGIETIAAKDNMILEI 249
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.140 0.410
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105139981
Number of Sequences: 2977
Number of extensions: 4426030
Number of successful extensions: 8046
Number of sequences better than 1.0e-10: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 8035
Number of HSP's gapped (non-prelim): 5
length of query: 270
length of database: 189,106,746
effective HSP length: 54
effective length of query: 216
effective length of database: 156,788,448
effective search space: 33866304768
effective search space used: 33866304768
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 164 (68.3 bits)