BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1306 (PAB1306) DE:putative, translation initiation factor
aIF-2BII translation initiation factor
(276 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UYB6|E2BL_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 543 e-154
sp|O58185|E2BL_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 453 e-126
gi|11499619 translation initiation factor eIF-2B, subunit delta ... 173 3e-42
sp|Q57586|E2B2_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 162 3e-39
sp|Q9V281|E2B2_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 147 1e-34
sp|O57947|E2B2_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 143 2e-33
gb|AAG20059.1| (AE005085) translation initiation factor eIF-2B s... 127 2e-28
gb|AAK43082.1| Translation initiation factor aiF-2B, subunit 1 (... 116 2e-25
sp|Q9X013|E2B_THEMA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 111 9e-24
gi|11497982 translation initiation factor eIF-2B, subunit delta ... 108 8e-23
gi|4503503 eukaryotic translation initiation factor 2B, subunit ... 107 1e-22
sp|Q9YE84|E2B1_AERPE PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 107 1e-22
gb|AAF02860.1|AC009324_9 (AC009324) Similar to initiation factor... 107 1e-22
sp|Q57896|E2B1_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 106 3e-22
gb|AAF27156.1|AC016529_19 (AC016529) putative translation initia... 104 1e-21
sp|Q64270|E2BA_RAT TRANSLATION INITIATION FACTOR EIF-2B ALPHA SU... 104 1e-21
sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 102 6e-21
gi|6322878 34 KD alpha subunit of eIF2B; Gcn3p [Saccharomyces ce... 101 1e-20
pir||T40858 translation initiation factor eif-2b alpha subunit -... 100 2e-20
emb|CAC12337.1| (AL445066) translation initiation factor aIF-2B,... 100 3e-20
gb|AAF56977.1| (AE003771) CG7883 gene product [Drosophila melano... 99 7e-20
gb|AAG38014.1| (AF289100) eukaryotic initiation factor eIF2B alp... 99 7e-20
gb|AAA68046.1| (M98035) guanine nucleotide exchange factor, eIF-... 94 2e-18
gi|6753736 eukaryotic translation initiation factor 2B [Mus musc... 94 2e-18
pir||A55146 guanine nucleotide exchange factor eIF-2B delta chai... 94 2e-18
sp|Q63186|E2BD_RAT TRANSLATION INITIATION FACTOR EIF-2B DELTA SU... 92 5e-18
emb|CAA53204.1| (X75451) translation initiation factor eIF-2B-de... 92 7e-18
sp|P41111|E2BD_RABIT TRANSLATION INITIATION FACTOR EIF-2B DELTA ... 92 7e-18
emb|CAB57260.1| (AJ011305) guanine nucleotide exchange factor, d... 90 3e-17
emb|CAB57261.1| (AJ011306) guanine nucleotide exchange factor (l... 90 3e-17
emb|CAB57305.1| (AJ011307) guanine nucleotide exchange factor [H... 90 3e-17
pir||T08757 probable translation initiation factor eIF-2B delta ... 90 3e-17
sp|O27900|E2B1_METTH PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 89 7e-17
sp|Q9UI10|E2BD_HUMAN TRANSLATION INITIATION FACTOR EIF-2B DELTA ... 88 1e-16
sp|Q09924|E2BD_SCHPO PROBABLE TRANSLATION INITIATION FACTOR EIF-... 87 2e-16
gb|AAD52847.2|AF137288_1 (AF137288) putative translation initiat... 87 2e-16
gb|AAF20216.1|AC012395_3 (AC012395) putative translation initiat... 86 4e-16
sp|O31662|E2B_BACSU PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 86 5e-16
gb|AAF46995.1| (AE003460) CG10315 gene product [Drosophila melan... 86 5e-16
gb|AAF02141.1|AC009853_1 (AC009853) putative translation initiat... 85 9e-16
dbj|BAB10154.1| (AB005231) contains similarity to guanine nucleo... 83 4e-15
sp|P34604|E2BA_CAEEL PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 82 6e-15
gb|AAG19758.1| (AE005061) translation initiation factor eIF-2B s... 81 1e-14
gb|AAF69705.1|AC016041_10 (AC016041) F27J15.24 [Arabidopsis thal... 80 3e-14
pir||T00687 hypothetical protein F6E13.20 - Arabidopsis thaliana... 77 2e-13
sp|O58433|E2B1_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 76 3e-13
sp|Q9UZ16|E2B1_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 74 2e-12
sp|Q9PAG5|E2B_XYLFA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 71 1e-11
gb|AAF57154.1| (AE003778) CG11334 gene product [alt 1] [Drosophi... 71 1e-11
>sp|Q9UYB6|E2BL_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2-LIKE
(EIF-2B GDP-GTP EXCHANGE FACTOR) >gi|7521496|pir||B75007
probable translation initiation factor aif-2bII
translation initiation factor PAB1306 - Pyrococcus
abyssi (strain Orsay) >gi|5459010|emb|CAB50496.1|
(AJ248288) putative, translation initiation factor
aIF-2BII translation initiation factor [Pyrococcus
abyssi]
Length = 276
Score = 543 bits (1384), Expect = e-154
Identities = 276/276 (100%), Positives = 276/276 (100%)
Query: 1 MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNP 60
MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNP
Sbjct: 1 MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNP 60
Query: 61 SMASLYNLARFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSS 120
SMASLYNLARFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSS
Sbjct: 61 SMASLYNLARFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSS 120
Query: 121 SVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATI 180
SVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATI
Sbjct: 121 SVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATI 180
Query: 181 SMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFS 240
SMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFS
Sbjct: 181 SMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFS 240
Query: 241 RSGFRVRNVLFDLTPWKFIRGIITELGIVIPPRDIQ 276
RSGFRVRNVLFDLTPWKFIRGIITELGIVIPPRDIQ
Sbjct: 241 RSGFRVRNVLFDLTPWKFIRGIITELGIVIPPRDIQ 276
>sp|O58185|E2BL_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2-LIKE
(EIF-2B GDP-GTP EXCHANGE FACTOR) >gi|7521497|pir||A71155
probable translation initiation factor eIF-2B -
Pyrococcus horikoshii >gi|3256843|dbj|BAA29526.1|
(AP000002) 276aa long hypothetical translation
initiation factor eIF-2B [Pyrococcus horikoshii]
Length = 276
Score = 453 bits (1152), Expect = e-126
Identities = 218/276 (78%), Positives = 254/276 (91%)
Query: 1 MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNP 60
MLP+RVLEIL EMK ERI+GA+WLA+KGAEAFL LAEELDE+LLEDAI ELR +++VNP
Sbjct: 1 MLPERVLEILREMKRERIKGASWLAKKGAEAFLTLAEELDESLLEDAIMELREEVVKVNP 60
Query: 61 SMASLYNLARFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSS 120
SMASLYNLARF+P+TN R+++K RALEFLRRM+EAKRELASIGAQLIDDGDVIITHSFSS
Sbjct: 61 SMASLYNLARFIPVTNRRDILKSRALEFLRRMEEAKRELASIGAQLIDDGDVIITHSFSS 120
Query: 121 SVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATI 180
+VLEI + AK+R+K FKVI+TESSPDYEGLHLA ELE GIEFEVITD+QMGLFCR+A+I
Sbjct: 121 TVLEIIRTAKERKKRFKVILTESSPDYEGLHLARELEFSGIEFEVITDAQMGLFCREASI 180
Query: 181 SMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFS 240
++VGADM+T+DG+VVNKAGTYLLALACHE+ +PFYVAAETYKFHPT+KS +V+L ERD
Sbjct: 181 AIVGADMITKDGYVVNKAGTYLLALACHENAIPFYVAAETYKFHPTLKSGDVMLMERDLI 240
Query: 241 RSGFRVRNVLFDLTPWKFIRGIITELGIVIPPRDIQ 276
R R+RNVLFD+TPWK++RGIITELGIVIPPRDIQ
Sbjct: 241 RGNVRIRNVLFDVTPWKYVRGIITELGIVIPPRDIQ 276
>gi|11499619 translation initiation factor eIF-2B, subunit delta (eif2BD)
[Archaeoglobus fulgidus]
>gi|3182986|sp|O28242|E2B2_ARCFU PUTATIVE TRANSLATION
INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447866|pir||D69504 translation
initiation factor eIF-2B, subunit delta (eif2BD) homolog
- Archaeoglobus fulgidus >gi|2648498|gb|AAB89217.1|
(AE000962) translation initiation factor eIF-2B, subunit
delta (eif2BD) [Archaeoglobus fulgidus]
Length = 313
Score = 173 bits (433), Expect = 3e-42
Identities = 101/283 (35%), Positives = 155/283 (54%), Gaps = 15/283 (5%)
Query: 4 DRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMA 63
+ VLE ++++ +RGA +AR AE + AE+ + ++ +R ++ P+
Sbjct: 2 EEVLEAAEKIRSMEVRGAARIARFAAETLMKFAEKASDEKFDEEMRFAAETLLNTRPTAV 61
Query: 64 SLYNLARFM------PITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHS 117
SLYN ++ + R+ V RA EF+ ++ A+R++ IG + I DG ++TH
Sbjct: 62 SLYNAINYVMRYSGESVEEKRQSVIRRAREFINWVETAQRKIGEIGEKRIKDGYTVMTHC 121
Query: 118 FSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRK 177
SS+ L + K A + K +VI TES P ++G +L GIE +I DS + F +
Sbjct: 122 NSSAALSVIKKAHENGKRVEVIATESRPRWQGHLTVKQLREAGIEVTLIVDSAVRYFINE 181
Query: 178 ATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHP-TVKSNEVVLHE 236
+VGAD +T +G ++NK GT +ALA E+ VPF VAAETYKF P T+ VV+ E
Sbjct: 182 VDCVVVGADTITANGALINKIGTSQIALAAKEARVPFMVAAETYKFSPKTLFGELVVIEE 241
Query: 237 RD--------FSRSGFRVRNVLFDLTPWKFIRGIITELGIVIP 271
RD + G +VRN FD+TP +I IITE+G + P
Sbjct: 242 RDAREVAPEEILKLGVKVRNPAFDVTPRDYIDVIITEIGAIPP 284
>sp|Q57586|E2B2_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2129320|pir||B64315
translation initiation factor eIF-2B delta chain homolog
- Methanococcus jannaschii >gi|1590893|gb|AAB98103.1|
(U67469) translation initiation factor aIF-2B, subunit
delta, putative [Methanococcus jannaschii]
Length = 308
Score = 162 bits (407), Expect = 3e-39
Identities = 102/284 (35%), Positives = 151/284 (52%), Gaps = 16/284 (5%)
Query: 4 DRVLEILNEMKAERIRGATWLARKGAEAFLALA---EELDEALLEDAIRELRSRIIEVNP 60
D + E ++K IRGA + R A+A A L+E ++ +RE + +I P
Sbjct: 9 DIIKETYEKIKNMEIRGAGRIGRAAAKALKEYALKISHLNEEEFKNKMREAGNILISARP 68
Query: 61 SMASLYNLARF----MPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITH 116
+ SL N+ ++ + N +E V RA EF+ +A + GA I DGD I+TH
Sbjct: 69 TAVSLPNVVKYVLKGLNEENPKERVIERADEFINSSLKAIENIGKFGANRIKDGDTILTH 128
Query: 117 SFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCR 176
S + + + K A D K KV TE+ P +G A L + GI+ +I DS + F +
Sbjct: 129 CNSEAAISVIKTAYDEGKDIKVFCTETRPRNQGYLTAKTLYDYGIDVTLIVDSAVRYFIK 188
Query: 177 KATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHP-TVKSNEVVLH 235
+ I +VGAD +T +G +VNK GT +AL +ES VPF AAETYKFHP T+ + +
Sbjct: 189 EIDIVVVGADAITANGCLVNKIGTSQIALIANESRVPFLTAAETYKFHPKTIVGELIEIE 248
Query: 236 ER--------DFSRSGFRVRNVLFDLTPWKFIRGIITELGIVIP 271
ER + G ++RN FD+TP K+I IITE+G++ P
Sbjct: 249 ERSPEEVAVFEDKYKGIKIRNPAFDVTPAKYIDAIITEVGLIPP 292
>sp|Q9V281|E2B2_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447876|pir||F75208
translation initiation factor aif-2bII translation
initiation factor PAB2402 - Pyrococcus abyssi (strain
Orsay) >gi|5457626|emb|CAB49117.1| (AJ248283)
translation initiation factor aIF-2BII translation
initiation factor [Pyrococcus abyssi]
Length = 324
Score = 147 bits (368), Expect = 1e-34
Identities = 101/298 (33%), Positives = 153/298 (50%), Gaps = 27/298 (9%)
Query: 1 MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSR---IIE 57
M+ V EI ++K+ IRGA +AR A+A + AE+ E+ EL+ + +
Sbjct: 4 MIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYK 63
Query: 58 VNPSMASLYNLARFM------PITNNRELVKMR------ALEFLRRMDEAKRELASIGAQ 105
P+ SL N R++ ++ +L +R A EF+ ++A + IGA+
Sbjct: 64 TRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAK 123
Query: 106 LIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEV 165
I+DGDVI+TH S + + + K A D+ K KVI+TE+ P ++G A EL + GI
Sbjct: 124 RIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIY 183
Query: 166 ITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHP 225
+ DS + + ++GAD +T +G V+NK GT L+AL E V +AAETYKFHP
Sbjct: 184 VVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHP 243
Query: 226 -TVKSNEVVLHERDFSR-----------SGFRVRNVLFDLTPWKFIRGIITELGIVIP 271
T+ V + RD + V N FD+TP ++I IITE GI+ P
Sbjct: 244 ETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPP 301
>sp|O57947|E2B2_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447865|pir||F71243
probable translation initiation factor eIF-2B delta -
Pyrococcus horikoshii >gi|3256594|dbj|BAA29277.1|
(AP000001) 324aa long hypothetical translation
initiation factor eIF-2B delta [Pyrococcus horikoshii]
Length = 324
Score = 143 bits (358), Expect = 2e-33
Identities = 100/298 (33%), Positives = 153/298 (50%), Gaps = 27/298 (9%)
Query: 1 MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELR--SRII-E 57
M+ V E ++K+ IRGA +AR A+A + AE+ E+ EL+ S+I+
Sbjct: 4 MIVKEVYETAEKIKSMEIRGAGRIARAAAQALMIQAEKSKAKEPEELWNELKVASKILYN 63
Query: 58 VNPSMASLYNLARFM------PITNNRELVKMR------ALEFLRRMDEAKRELASIGAQ 105
P+ SL N R++ +L +R A EF+ ++A + IGA+
Sbjct: 64 TRPTAVSLPNALRYVMHRVKAAYLGGADLETLRFTAINSAKEFIYNSEKAIERIGEIGAK 123
Query: 106 LIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEV 165
I+DGD+I+TH S + + + K A ++ K+ KVI+TE+ P ++G A EL + GI
Sbjct: 124 RIEDGDIIMTHCHSKAAISVMKKAFEQGKNIKVIVTETRPKWQGKITAKELASYGIPVIY 183
Query: 166 ITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHP 225
I DS + + ++GAD +T +G V+NK GT L+AL E V +AAETYKFHP
Sbjct: 184 IVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHP 243
Query: 226 TVKSNEVV-LHERDFSR-----------SGFRVRNVLFDLTPWKFIRGIITELGIVIP 271
++V + RD + V N FD+TP ++I IITE GI+ P
Sbjct: 244 ATMLGQLVEIEMRDPTEVIPEEELRTWPKNIEVWNPAFDVTPPEYIDVIITERGIIPP 301
>gb|AAG20059.1| (AE005085) translation initiation factor eIF-2B subunit alpha;
Eif2ba [Halobacterium sp. NRC-1]
Length = 314
Score = 127 bits (315), Expect = 2e-28
Identities = 89/296 (30%), Positives = 142/296 (47%), Gaps = 22/296 (7%)
Query: 1 MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDE---ALLEDAIRELRSRIIE 57
M+ + V +++ IRGA +AR A+A + A + D A +R + +
Sbjct: 1 MVTEDVRATADDIADMEIRGAATIARAAADALESQATDSDADSPAAFRAELRAAARHLRD 60
Query: 58 VNPSMASLYNLARF-MPITNNRELVKMRAL------EFLRRMDEAKRELASIGAQLIDDG 110
P+ SL N R+ + T+ + +RA F +++D A+ +L +IGA + DG
Sbjct: 61 TRPTAVSLPNALRYVLGNTDGETVAALRASVTDAADAFRQQLDRAQDDLGAIGANRLRDG 120
Query: 111 DVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQ 170
D ++TH S+ L K A D K + ++ E+ P +G A++L G+ +I D+
Sbjct: 121 DTVMTHCHSTDALACVKAALDDGKHIEAVVKETRPRKQGHITADQLREWGVPVTLIVDNA 180
Query: 171 MGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHP-TVKS 229
+ +VGAD + DG V+NK GT +LA+ E VP VAA+T K HP T+
Sbjct: 181 GRRYLDAVDHVLVGADSIAADGSVINKIGTSMLAVVARERGVPVMVAAQTLKLHPDTLTG 240
Query: 230 NEVVLHERDFSR----------SGFRVRNVLFDLTPWKFIRGIITELGIVIPPRDI 275
N V + RD + V N FD+TP +++ I+TE G PP I
Sbjct: 241 NTVEIEMRDETEVLAAADRHQLGAPDVENPAFDVTPPRYVDAIVTERG-QFPPESI 295
>gb|AAK43082.1| Translation initiation factor aiF-2B, subunit 1 (eiF2B) [Sulfolobus
solfataricus]
Length = 366
Score = 116 bits (289), Expect = 2e-25
Identities = 95/301 (31%), Positives = 144/301 (47%), Gaps = 41/301 (13%)
Query: 13 MKAERIRGATWLA-RKGAEAFLALAEELDEALLEDAIREL-RSRII--EVNPSMASL-YN 67
+K ++RGA + G LAL + L++AIREL R++ I P+ +L +
Sbjct: 55 IKNMQVRGAPAIGITAGYGMVLALTSNKNVKTLDEAIRELTRAKAILDSARPTAVNLVWA 114
Query: 68 LARFMPITNN----------RELVKMRALEFLRRMDE---AKRELASIGAQLIDDGDVII 114
+R + + N EL+ + +E R DE A+ ++ G + ++D D ++
Sbjct: 115 TSRMLTLAKNAVENGNAKTINELIDLMKVEAKRIFDEEYDAEIQMGLYGLEKLNDSDTVL 174
Query: 115 THSFSSSV---------LEIFKVAKDRRKSFKVIITESSPDYEGLHLA-NELENLGIEFE 164
T + + L K+AK + VI E+ P +G L EL GI+
Sbjct: 175 TQCNAGGLATGTGLGTALSPVKLAKALGMNVSVIAPETRPWLQGSRLTVYELMEEGIKVT 234
Query: 165 VITDSQMGLFCRKATIS--MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYK 222
+ITD+ +GL + ++ MVGAD + RDG V NK GTY A+ HE +PFY A T
Sbjct: 235 LITDTAVGLVMYRGMVNSVMVGADRILRDGHVFNKIGTYKEAVIAHELGIPFYALAPTSS 294
Query: 223 FHPTVKSNEVVLHERD-----------FSRSGFRVRNVLFDLTPWKFIRGIITELGIVIP 271
F NE+ + ERD + V N +FD+TP K+I GIITE GI+ P
Sbjct: 295 FDLKSNVNEIKIEERDPNEVRTIRGIPIAPENVNVYNPVFDVTPPKYIAGIITEKGIIYP 354
Query: 272 P 272
P
Sbjct: 355 P 355
>sp|Q9X013|E2B_THEMA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447871|pir||F72319 translation
initiation factor IF-2B alpha subunit-related TM0911
[similarity] - Thermotoga maritima (strain MSB8)
>gi|4981447|gb|AAD35992.1|AE001755_15 (AE001755)
translation initiation factor, aIF-2B alpha
subunit-related [Thermotoga maritima]
Length = 343
Score = 111 bits (275), Expect = 9e-24
Identities = 85/296 (28%), Positives = 138/296 (45%), Gaps = 31/296 (10%)
Query: 8 EILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASLY- 66
E+ + +K +RGA + A ++ + L D +++++ + P+ +L+
Sbjct: 37 EVAHAIKEMIVRGAPAIGVAAAFGYVLGLRDYKTGSLTDWMKQVKETLARTRPTAVNLFW 96
Query: 67 NLARFMPI-------TNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFS 119
L R + N E+++ AL+ E + + GAQLI DG I+TH +
Sbjct: 97 ALNRMEKVFFENADRENLFEILENEALKMAYEDIEVNKAIGKNGAQLIKDGSTILTHCNA 156
Query: 120 SSV--------LEIFKVAKDRRKSFKVIITESSPDYEGLHL-ANELENLGIEFEVITDSQ 170
++ L + + A + K +V E+ P +G L A EL GIE VITD+
Sbjct: 157 GALATVDYGTALGVIRAAVESGKRIRVFADETRPYLQGARLTAWELMKDGIEVYVITDNM 216
Query: 171 MGLFCRKATIS--MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVK 228
G ++ I +VGAD + +G NK GTY LA+ +++PFYVAA PT++
Sbjct: 217 AGWLMKRGLIDAVVVGADRIALNGDTANKIGTYSLAVLAKRNNIPFYVAAPVSTIDPTIR 276
Query: 229 S-NEVVLHER-----------DFSRSGFRVRNVLFDLTPWKFIRGIITELGIVIPP 272
S E+ + ER + G +V N FD+T I IITE G++ PP
Sbjct: 277 SGEEIPIEERRPEEVTHCGGNRIAPEGVKVLNPAFDVTENTLITAIITEKGVIRPP 332
>gi|11497982 translation initiation factor eIF-2B, subunit delta (eif2BD)
[Archaeoglobus fulgidus]
>gi|3182987|sp|O29877|E2B1_ARCFU PUTATIVE TRANSLATION
INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447868|pir||B69296 translation
initiation factor eIF-2B, subunit delta (eif2BD) homolog
- Archaeoglobus fulgidus >gi|2650263|gb|AAB90865.1|
(AE001079) translation initiation factor eIF-2B, subunit
delta (eif2BD) [Archaeoglobus fulgidus]
Length = 339
Score = 108 bits (267), Expect = 8e-23
Identities = 86/303 (28%), Positives = 145/303 (47%), Gaps = 34/303 (11%)
Query: 6 VLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSR---IIEVNPSM 62
V E+ + +K +RGA L GA A E + A +++ L+ + P+
Sbjct: 30 VEELADAIKKLAVRGAPALEAAGAYGIALAAREREFADVDELKEHLKKAADFLASTRPTA 89
Query: 63 ASLY-NLARFMPITNNRELVK------MRALEFLRRMD-EAKRELASIGAQLIDDGDVII 114
+L+ + R + E V+ +R E L D E R++ GA+L++DGDV++
Sbjct: 90 VNLFVGIERALNAALKGESVEEVKELALREAEKLAEEDVERNRKMGEYGAELLEDGDVVL 149
Query: 115 THSFSS--------SVLEIFKVAKDRRKSFKVIITESSPDYEGLHLAN-ELENLGIEFEV 165
T+ + + L + + A ++ K +VI E+ P +G L EL GI+ +
Sbjct: 150 TYCNAGRLATVDWGTALGVVRSAVEQGKEIRVIACETRPLNQGSRLTCWELMEDGIDVTL 209
Query: 166 ITDSQMGLFCRKATIS--MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKF 223
ITDS +G+ +K + +VGAD + RD V NK GTY +++ ++PFYVAA F
Sbjct: 210 ITDSMVGIVMQKGMVDKVIVGADRIVRDA-VFNKIGTYTVSVVAKHHNIPFYVAAPKATF 268
Query: 224 HPTVKSNEVVLHE-----------RDFSRSGFRVRNVLFDLTPWKFIRGIITELGIVIPP 272
+ +VV+ E R + +V N FD TP + + +ITE G++ PP
Sbjct: 269 DWERTAKDVVIEERPREELIFCGKRQIAPLNVKVYNPAFDPTPLENVTALITEYGVIYPP 328
Query: 273 RDI 275
++
Sbjct: 329 YEV 331
>gi|4503503 eukaryotic translation initiation factor 2B, subunit 1 (alpha,
26kD); eukaryotic translation initiation factor 2B;
eIF-2-beta [Homo sapiens] >gi|11433185|ref|XP_006566.1|
eukaryotic translation initiation factor 2B, subunit 1
(alpha, 26kD) [Homo sapiens]
>gi|2494303|sp|Q14232|E2BA_HUMAN TRANSLATION INITIATION
FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B GDP-GTP EXCHANGE
FACTOR) >gi|1200232|emb|CAA64950.1| (X95648) alfa
subunit [Homo sapiens]
Length = 305
Score = 107 bits (265), Expect = 1e-22
Identities = 79/276 (28%), Positives = 132/276 (47%), Gaps = 22/276 (7%)
Query: 6 VLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASL 65
+LE L K E I+G E + D ++ + EL R I S+ASL
Sbjct: 30 LLEFLKRDKGETIQGLRANLTSAIETLCGV----DSSVAVSSGGELFLRFI----SLASL 81
Query: 66 YNLARFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEI 125
+ + ++++ R FLRR+ ++ ++A + I DG I+TH++S VL +
Sbjct: 82 ----EYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRV 137
Query: 126 FKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGA 185
+ A +K F V +TES PD G +A L +L + V+ D+ +G KA + +VGA
Sbjct: 138 LEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGA 197
Query: 186 DMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHER-DFSRSGF 244
+ V +G ++NK GT +A+ + PFYV AE++KF N+ + ++ +
Sbjct: 198 EGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTL 257
Query: 245 RVRNVLFDLT---PW------KFIRGIITELGIVIP 271
+V DL PW I + T+LG++ P
Sbjct: 258 KVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTP 293
>sp|Q9YE84|E2B1_AERPE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447874|pir||F72657
probable translation initiation factor eIF-2B alpha
subunit APE0686 [imported] - Aeropyrum pernix (strain
K1) >gi|5104347|dbj|BAA79662.1| (AP000060) 370aa long
hypothetical translation initiation factor eFI-2B alpha
subunit [Aeropyrum pernix]
Length = 370
Score = 107 bits (265), Expect = 1e-22
Identities = 88/300 (29%), Positives = 134/300 (44%), Gaps = 44/300 (14%)
Query: 18 IRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASLYNL----ARFMP 73
IRGA + A A A E E I L E+ + + YNL AR
Sbjct: 65 IRGAPAIGVAAAYALALAAAEAASRGGEGFIEALSEARREIESTRPTAYNLFWATARVYG 124
Query: 74 ITNNRELVKMRALEFLRR--MDEAKR----------ELASIGAQLIDDGDVIITHSFSSS 121
+ E + ++ R ++EA R E+ +GA+L++ GD ++TH + +
Sbjct: 125 AVS--EAFRRSGVDAAVRAGLEEATRIYVEDVRGNVEIGRVGARLLESGDTVLTHCNTGA 182
Query: 122 V--------LEIFKVAKDRRKSFKVIITESSPDYEGLHL-ANELENLGIEFEVITDSQMG 172
+ L + + A K +VI TE+ P +G L EL GI F+++ DS +G
Sbjct: 183 LATAGFGTALGVIRYAWMEGKDIRVITTETRPVLQGARLNVWELRKEGIPFKLVVDSAVG 242
Query: 173 LFCRKATIS--MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSN 230
L + +S +VGAD + G NK GTY++A+A VPFYVAA F P
Sbjct: 243 LIMSRGMVSKAIVGADRIVSTGHTANKIGTYMVAMAASRHGVPFYVAAPASTFQPDAGPE 302
Query: 231 EVVLHERD-------FSRSGF--------RVRNVLFDLTPWKFIRGIITELGIVIPPRDI 275
+V+ ER S +G+ N FD+TP + I IT+ G++ PP D+
Sbjct: 303 AIVIEERSPDEVRGVISEAGYVRITLGDVEAYNPSFDVTPPELITAFITDRGVIEPPFDV 362
>gb|AAF02860.1|AC009324_9 (AC009324) Similar to initiation factor 2 subunit [Arabidopsis
thaliana] >gi|6056397|gb|AAF02861.1|AC009324_10
(AC009324) Similar to initiation factor 2 subunit
[Arabidopsis thaliana]
Length = 351
Score = 107 bits (265), Expect = 1e-22
Identities = 71/201 (35%), Positives = 100/201 (49%), Gaps = 11/201 (5%)
Query: 82 KMRALEFLRRMDE----AKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
K R LE + E A+ +A + I DG I+ H FS V EI K + +K F+
Sbjct: 139 KSRVLERAEKFGEISCKARTIIAMLSQDFIFDGCTILVHGFSRVVFEILKTSAQNKKLFR 198
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V+ TE PD G+ LANEL L I +++ DS + + + VGAD V G ++N
Sbjct: 199 VLCTEGRPDKTGVLLANELAKLDIPVKLLIDSAVAYSMDEVDMVFVGADGVVESGGIINM 258
Query: 198 AGTYLLALACHESDVPFYVAAETYKF---HP-TVKSNEVVLHERDFS---RSGFRVRNVL 250
GTY +AL + P YVAAE+YKF +P K E L DFS V
Sbjct: 259 MGTYQIALVAQSMNKPVYVAAESYKFARLYPLDQKDLEPALRPIDFSVPVPPKVEVERSA 318
Query: 251 FDLTPWKFIRGIITELGIVIP 271
D TP +++ + T+LG++ P
Sbjct: 319 RDYTPPQYLTLLFTDLGVLTP 339
>sp|Q57896|E2B1_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2129325|pir||F64356
translation initiation factor aIF-2B alpha chain -
Methanococcus jannaschii >gi|1591158|gb|AAB98443.1|
(U67496) translation initiation factor aIF-2B, subunit
delta (aIF2BD) [Methanococcus jannaschii]
Length = 329
Score = 106 bits (262), Expect = 3e-22
Identities = 75/203 (36%), Positives = 97/203 (46%), Gaps = 22/203 (10%)
Query: 94 EAKRELASIGAQLIDDGDVIITH--------SFSSSVLEIFKVAKDRRKSFKVIITESSP 145
E +++ IG +LI+DGD I+TH S + L + + A K +VI E+ P
Sbjct: 120 ETCKKIGMIGEKLIEDGDTILTHCNAGALATSAYGTALSVIRFAFYNGKKIRVIADETRP 179
Query: 146 DYEGLHL-ANELENLGIEFEVITDSQMGLFCRKATIS--MVGADMVTRDGFVVNKAGTYL 202
+G L A EL GI +VITD+ G +K I +VGAD + DG V NK GTY
Sbjct: 180 RLQGAKLTAFELNYEGIPVKVITDNTAGFLMQKGEIDKIIVGADRILADGTVYNKIGTYS 239
Query: 203 LALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFSR-----------SGFRVRNVLF 251
LA+ +PFYVAA F +V++ ERD G N F
Sbjct: 240 LAVLAKYHRIPFYVAAPLSTFDLRSSEEDVIIEERDEKEVAYIDGVRIVPEGVGCYNYAF 299
Query: 252 DLTPWKFIRGIITELGIVIPPRD 274
D TP I IITE GIV P RD
Sbjct: 300 DKTPPDLITAIITEKGIVKPNRD 322
>gb|AAF27156.1|AC016529_19 (AC016529) putative translation initiation factor EIF-2B alpha
subunit; 76271-74747 [Arabidopsis thaliana]
Length = 362
Score = 104 bits (257), Expect = 1e-21
Identities = 69/201 (34%), Positives = 100/201 (49%), Gaps = 11/201 (5%)
Query: 82 KMRALEFLRRMDE----AKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
K R LE + E A+R +A + I DG I+ H S VLEI K A K F+
Sbjct: 150 KSRLLERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGLSRVVLEILKTAAQNNKLFR 209
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V+ TE PD G+ L++EL L I +++ DS + + + VGAD V G ++N
Sbjct: 210 VLCTEGRPDGTGVLLSSELSKLDIPVKLLLDSAVAYSMDEVDMVFVGADGVVESGGIINM 269
Query: 198 AGTYLLALACHESDVPFYVAAETYKF---HP-TVKSNEVVLHERDFS---RSGFRVRNVL 250
GTY +AL H + P YVAAE+YKF +P K L +F + V
Sbjct: 270 MGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIEFGVKIPTKVEVERSA 329
Query: 251 FDLTPWKFIRGIITELGIVIP 271
D TP +++ + T+LG++ P
Sbjct: 330 RDYTPPQYLTLLFTDLGVLSP 350
>sp|Q64270|E2BA_RAT TRANSLATION INITIATION FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|2144003|pir||I59376 translation
initiation factor eIF-2B alpha chain [validated] - rat
>gi|623033|gb|AAC52196.1| (U05821) translation
initiation factor eIF-2B alpha-subunit [Rattus
norvegicus] >gi|1050328|gb|AAA91276.1| (L41679)
GTP-exchange protein [Rattus norvegicus]
>gi|1096885|prf||2112359A initiation factor eIF-2B
[Rattus norvegicus]
Length = 305
Score = 104 bits (257), Expect = 1e-21
Identities = 80/277 (28%), Positives = 134/277 (47%), Gaps = 24/277 (8%)
Query: 6 VLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASL 65
+LE L K E I+G K E A+ D ++ + EL R I S+ SL
Sbjct: 30 LLEFLKRDKGETIQGLRAHLTKAIETLCAV----DSSVAVSSGGELFLRFI----SLTSL 81
Query: 66 YNLARFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEI 125
+ + ++++ R FL R+ ++ ++AS+ I DG I+TH++S VL +
Sbjct: 82 ----EYSDYSKCKKIMIERGELFLSRISLSRTKIASLCHAFIKDGARILTHAYSRVVLRV 137
Query: 126 FKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGA 185
+ A +K F V ITES PD G +A L +L + V+ D+ +G K + +VGA
Sbjct: 138 LEEAVAAKKRFSVYITESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKVDLVIVGA 197
Query: 186 DMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFSRSGFR 245
+ V +G ++NK GT +A+ + PFYV AE++KF N+ + ++ F
Sbjct: 198 EGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDK-FKYKADT 256
Query: 246 VRNV-----LFDLTPW------KFIRGIITELGIVIP 271
+++V L + PW I + T+LG++ P
Sbjct: 257 LKSVQAGQDLKEEHPWVDYTSPSLITLLFTDLGVLTP 293
>sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7519844|pir||C70481 translation
initiation factor eIF-2B alpha chain - Aquifex aeolicus
>gi|2984318|gb|AAC07840.1| (AE000773) initiation factor
eIF-2B alpha subunit [Aquifex aeolicus]
Length = 456
Score = 102 bits (251), Expect = 6e-21
Identities = 82/290 (28%), Positives = 134/290 (45%), Gaps = 29/290 (10%)
Query: 8 EILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASLY- 66
E+ +K +RGA + A F+ L + ++ ++ + P+ +L+
Sbjct: 165 EVAKAIKEMAVRGAPAIGCTAAYGFV-----LGVKVQKEDPEKVYETLKNTRPTAYNLFW 219
Query: 67 NLARFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSV---- 122
L R M + ++ A E + EA + + IG++++ ++TH + ++
Sbjct: 220 ALDRMMKALKEGKDIEEEAKEIEKEDYEANKRMGEIGSEIVPLNAKVLTHCNTGALATAG 279
Query: 123 ----LEIFKVAKDRRKSFKVIITESSPDYEGLHL-ANELENLGIEFEVITDSQMGLFCRK 177
L + + A K+ V + E+ P +G L A EL I ++ITDS G +K
Sbjct: 280 WGTALGVIRSAHYGGKNIFVWVDETRPYLQGARLTAWELVKESIPHKIITDSTAGFLMKK 339
Query: 178 ATISMV--GADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKS-NEVVL 234
+ +V GAD +T G V NK GTY LA+ C E +VPFYVAA T +KS E+++
Sbjct: 340 GMVDLVIVGADRITAKGDVANKIGTYTLAVLCKEHNVPFYVAAPTSTIDSNIKSGEEIII 399
Query: 235 HERD-----------FSRSGFRVRNVLFDLTPWKFIRGIITELGIVIPPR 273
ER + ++ FD+TP + I GIITE GI P R
Sbjct: 400 EERSPEEVKVCGGCAIAPKESDALHLAFDITPAELITGIITEKGIFKPER 449
>gi|6322878 34 KD alpha subunit of eIF2B; Gcn3p [Saccharomyces cerevisiae]
>gi|121065|sp|P14741|E2BA_YEAST TRANSLATION INITIATION
FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B GDP-GTP EXCHANGE
FACTOR) (GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT
GCN3) (GCD COMPLEX SUBUNIT GCN3) (TRANSCRIPTIONAL
ACTIVATOR GCN3) >gi|83571|pir||A31562 guanine nucleotide
exchange factor eIF-2B delta chain [validated] - yeast
(Saccharomyces cerevisiae) >gi|171580|gb|AAA34637.1|
(M23356) transcriptional activator protein GCN3
[Saccharomyces cerevisiae] >gi|486453|emb|CAA82098.1|
(Z28251) ORF YKR026c [Saccharomyces cerevisiae]
Length = 305
Score = 101 bits (248), Expect = 1e-20
Identities = 59/192 (30%), Positives = 100/192 (51%), Gaps = 8/192 (4%)
Query: 88 FLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDY 147
F+ R +++ ++A IG I D D+I+ H +S +V + A ++ F+ ++TES P
Sbjct: 101 FVSRAKKSRNKIAEIGVDFIADDDIILVHGYSRAVFSLLNHAANKFIRFRCVVTESRPSK 160
Query: 148 EGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALAC 207
+G L LE GI +I DS +G K VGA+ V G ++N GTY + +
Sbjct: 161 QGNQLYTLLEQKGIPVTLIVDSAVGAVIDKVDKVFVGAEGVAESGGIINLVGTYSVGVLA 220
Query: 208 HESDVPFYVAAETYKFHP--TVKSNEVVL--HERDFSRSGFRVRNVL----FDLTPWKFI 259
H + PFYV E++KF + S+++ + DF+R + + L D T ++I
Sbjct: 221 HNARKPFYVVTESHKFVRMFPLSSDDLPMAGPPLDFTRRTDDLEDALRGPTIDYTAQEYI 280
Query: 260 RGIITELGIVIP 271
+IT+LG++ P
Sbjct: 281 TALITDLGVLTP 292
>pir||T40858 translation initiation factor eif-2b alpha subunit - fission yeast
(Schizosaccharomyces pombe) >gi|6016995|emb|CAB57849.1|
(AL121783) translation initiation factor eif-2b alpha
subunit [Schizosaccharomyces pombe]
Length = 341
Score = 100 bits (247), Expect = 2e-20
Identities = 67/229 (29%), Positives = 106/229 (46%), Gaps = 44/229 (19%)
Query: 78 RELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
R LV+ L F++R ++ +A +G LI DG VI+TH FS V + A R FK
Sbjct: 105 RHLVENGKL-FIQRARACRQRIAHLGYPLIRDGSVILTHGFSRGVAAVLLAAAKRHVRFK 163
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V +TES P G + L+N I ++ DS + + + +VGA+ V +G ++N+
Sbjct: 164 VFVTESRPSGSGCLMTRTLKNACIPTCMVLDSAVSFTMNRVDLVLVGAEGVVENGGLINQ 223
Query: 198 AGTYLLALACHESDVPFYVAAETYKF----------------------------HPTVK- 228
GT+ LA+ + PFY AE++KF HP +
Sbjct: 224 IGTFQLAVFAKHAHKPFYAVAESHKFVRMFPLSQYDIPFSRPILEFDDPSPETVHPEPEP 283
Query: 229 --------SNEVVLHERDFSRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
NE++++E N D+TP +F+ G+IT+LGI+
Sbjct: 284 IPTPSDAIHNELIMNEEQIR------NNPTLDVTPPEFVSGLITDLGII 326
>emb|CAC12337.1| (AL445066) translation initiation factor aIF-2B, alpha chain
related protein [Thermoplasma acidophilum]
Length = 340
Score = 99.8 bits (245), Expect = 3e-20
Identities = 85/288 (29%), Positives = 142/288 (48%), Gaps = 34/288 (11%)
Query: 8 EILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASLYN 67
+I +K +RGA + A LA+A++ E + ++A+R++ S P+ L+
Sbjct: 44 DIAYAIKNMVVRGAPAIGVTAAYG-LAMAKKKGENM-DEAVRKIGS----TRPTAYDLFK 97
Query: 68 LARFMPITN-NRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEI- 125
R+M I + + + A+E R R++ IG QLI I+TH + ++ +
Sbjct: 98 AIRYMQINDFDMNSARRYAMEISGR----SRKIGEIGNQLIGQNARILTHCNAGALAVVD 153
Query: 126 -------FKVAKDRRKSFKVIITESSPDYEGLHL-ANELENLGIEFEVITDSQMGLFCRK 177
++A K+ V + E+ P +G L A EL GI+ +I D+ G + R+
Sbjct: 154 WGTALAPIRMAHGDGKNIFVFVDETRPRLQGAKLTAWELAQEGIDHAIIADNAAGFYMRR 213
Query: 178 ATISMV--GADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKS-NEVVL 234
+ +V GAD + +G NK GTY A+ + +PFYVAA F ++KS +E+ +
Sbjct: 214 KEVDLVIVGADRIASNGDFANKIGTYEKAVLAKTNGIPFYVAAPGSTFDFSIKSGDEIPI 273
Query: 235 HERDFSR----SGFRV-------RNVLFDLTPWKFIRGIITELGIVIP 271
ERD + +G R+ RN FD+TP +++ G ITE GI P
Sbjct: 274 EERDENEVLEINGTRIGPVESHSRNPAFDVTPNEYVTGFITEYGIFKP 321
>gb|AAF56977.1| (AE003771) CG7883 gene product [Drosophila melanogaster]
Length = 306
Score = 98.7 bits (242), Expect = 7e-20
Identities = 68/239 (28%), Positives = 116/239 (48%), Gaps = 11/239 (4%)
Query: 44 LEDAIRELRSRIIEVNPSMASLYNLARFMPITNN-------RELVKMRALEFLRRMDEAK 96
+ +A+ +R+ + + +++ RF+ ++ + R+++ R FL ++ ++
Sbjct: 56 MREAVAAMRNTDLSIAAIVSAGELFCRFITLSLDDKHMEECRQIMLNRGKIFLTKLLNSR 115
Query: 97 RELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANEL 156
+ +A + I DG I+THS S VL+ A +KSF V +T+ G + +L
Sbjct: 116 QVIAQQAQRFITDGCRILTHSRSRVVLKALITASQNKKSFHVYVTQGGTGNSGEEMVKDL 175
Query: 157 ENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYV 216
GI+ +I DS G +VGA+ V G ++N+ GTY + L E PFYV
Sbjct: 176 HAAGIDCTLILDSATGYVMESVDFVLVGAEAVVESGGIINRIGTYTMGLCAREMKKPFYV 235
Query: 217 AAETYKFHPTVKSNEVVL-HERDFSRSGFR-VRNV--LFDLTPWKFIRGIITELGIVIP 271
AE++KF N+ L +E +SR V V L D TP +I + T+LG + P
Sbjct: 236 LAESFKFSRLYPLNQRDLPNEYKYSRKHLNDVSKVHPLVDYTPPVYITLLFTDLGRLTP 294
>gb|AAG38014.1| (AF289100) eukaryotic initiation factor eIF2B alpha subunit
[Drosophila melanogaster]
Length = 300
Score = 98.7 bits (242), Expect = 7e-20
Identities = 68/239 (28%), Positives = 116/239 (48%), Gaps = 11/239 (4%)
Query: 44 LEDAIRELRSRIIEVNPSMASLYNLARFMPITNN-------RELVKMRALEFLRRMDEAK 96
+ +A+ +R+ + + +++ RF+ ++ + R+++ R FL ++ ++
Sbjct: 50 MREAVAAMRNTDLSIAAIVSAGELFCRFITLSLDDKHMEECRQIMLNRGKIFLTKLLNSR 109
Query: 97 RELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANEL 156
+ +A + I DG I+THS S VL+ A +KSF V +T+ G + +L
Sbjct: 110 QVIAQQAQRFITDGCRILTHSRSRVVLKALITASQNKKSFHVYVTQGGTGNSGEEMVKDL 169
Query: 157 ENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYV 216
GI+ +I DS G +VGA+ V G ++N+ GTY + L E PFYV
Sbjct: 170 HAAGIDCTLILDSATGYVMESVDFVLVGAEAVVESGGIINRIGTYTMGLCAREMKKPFYV 229
Query: 217 AAETYKFHPTVKSNEVVL-HERDFSRSGFR-VRNV--LFDLTPWKFIRGIITELGIVIP 271
AE++KF N+ L +E +SR V V L D TP +I + T+LG + P
Sbjct: 230 LAESFKFSRLYPLNQRDLPNEYKYSRKHLNDVSKVHPLVDYTPPVYITLLFTDLGRLTP 288
>gb|AAA68046.1| (M98035) guanine nucleotide exchange factor, eIF-2B, delta subunit
[Mus musculus]
Length = 544
Score = 93.6 bits (229), Expect = 2e-18
Identities = 71/262 (27%), Positives = 119/262 (45%), Gaps = 33/262 (12%)
Query: 41 EALLEDAIRELR---SRIIEVNPSMASLYNLARF-------MPITNNRELVKMRALEFLR 90
E L D + +L+ S + + P AS+ N +F M + E K E L
Sbjct: 267 EELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELKEALD 326
Query: 91 RMDEAKRELAS-----IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSP 145
R + K LA+ + I DGDVI+ + SS V I + A+ + F+V++ +S P
Sbjct: 327 RYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRP 386
Query: 146 DYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLAL 205
EG H+ + L G+ + + + ++GA + +G V+++ GT LAL
Sbjct: 387 RLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLAL 446
Query: 206 ACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------FSRSGFRVR 247
+VP V ETYKF V+++ V +E D S R+
Sbjct: 447 VARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGDQVALANWQSHPSLRLL 506
Query: 248 NVLFDLTPWKFIRGIITELGIV 269
N+++D+TP + + +ITELG++
Sbjct: 507 NLVYDVTPPELVDLVITELGMI 528
>gi|6753736 eukaryotic translation initiation factor 2B [Mus musculus]
>gi|2494307|sp|Q61749|E2BD_MOUSE TRANSLATION INITIATION
FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP EXCHANGE
FACTOR) >gi|1083345|pir||B55146 guanine nucleotide
exchange factor eIF-2B delta chain, short form - mouse
>gi|529430|gb|AAA68047.1| (M98036) guanine nucleotide
exchange factor, eIF-2B, delta subunit [Mus musculus]
Length = 524
Score = 93.6 bits (229), Expect = 2e-18
Identities = 71/262 (27%), Positives = 119/262 (45%), Gaps = 33/262 (12%)
Query: 41 EALLEDAIRELR---SRIIEVNPSMASLYNLARF-------MPITNNRELVKMRALEFLR 90
E L D + +L+ S + + P AS+ N +F M + E K E L
Sbjct: 247 EELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELKEALD 306
Query: 91 RMDEAKRELAS-----IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSP 145
R + K LA+ + I DGDVI+ + SS V I + A+ + F+V++ +S P
Sbjct: 307 RYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRP 366
Query: 146 DYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLAL 205
EG H+ + L G+ + + + ++GA + +G V+++ GT LAL
Sbjct: 367 RLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLAL 426
Query: 206 ACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------FSRSGFRVR 247
+VP V ETYKF V+++ V +E D S R+
Sbjct: 427 VARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGDQVALANWQSHPSLRLL 486
Query: 248 NVLFDLTPWKFIRGIITELGIV 269
N+++D+TP + + +ITELG++
Sbjct: 487 NLVYDVTPPELVDLVITELGMI 508
>pir||A55146 guanine nucleotide exchange factor eIF-2B delta chain, long form -
mouse
Length = 544
Score = 93.6 bits (229), Expect = 2e-18
Identities = 71/262 (27%), Positives = 119/262 (45%), Gaps = 33/262 (12%)
Query: 41 EALLEDAIRELR---SRIIEVNPSMASLYNLARF-------MPITNNRELVKMRALEFLR 90
E L D + +L+ S + + P AS+ N +F M + E K E L
Sbjct: 267 EELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELREALD 326
Query: 91 RMDEAKRELAS-----IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSP 145
R + K LA+ + I DGDVI+ + SS V I + A+ + F+V++ +S P
Sbjct: 327 RYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRP 386
Query: 146 DYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLAL 205
EG H+ + L G+ + + + ++GA + +G V+++ GT LAL
Sbjct: 387 RLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLAL 446
Query: 206 ACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------FSRSGFRVR 247
+VP V ETYKF V+++ V +E D S R+
Sbjct: 447 VARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGDQVALANWQSHPSLRLL 506
Query: 248 NVLFDLTPWKFIRGIITELGIV 269
N+++D+TP + + +ITELG++
Sbjct: 507 NLVYDVTPPELVDLVITELGMI 528
>sp|Q63186|E2BD_RAT TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|1083768|pir||S52373 translation
initiation factor eIF-2B delta chain - rat
>gi|7514109|pir||S71961 translation initiation factor
eIF-2B delta chain - rat >gi|667056|emb|CAA88256.1|
(Z48225) protein synthesis initiation factor eIF-2B
delta subunit [Rattus norvegicus]
Length = 524
Score = 92.4 bits (226), Expect = 5e-18
Identities = 71/263 (26%), Positives = 121/263 (45%), Gaps = 33/263 (12%)
Query: 40 DEALLEDAIRELR---SRIIEVNPSMASLYNLARF-------MPITNNRELVKMRALEFL 89
+E L D + +L+ S + + P AS+ N +F M + E K E +
Sbjct: 246 NEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFFNKEVTGMSSSKREEEAKSELKEAI 305
Query: 90 RRMDEAKRELAS-----IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESS 144
R + K LAS ++ I DGDVI+ + SS V I + A + F+V++ +S
Sbjct: 306 DRYVQEKIVLASQAISRFASKKISDGDVILVYGCSSLVSRILQEAWVEGRRFRVVVVDSR 365
Query: 145 PDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLA 204
P EG H+ + L G+ + + + ++GA + +G V+++ GT LA
Sbjct: 366 PRLEGRHMLHCLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLA 425
Query: 205 LACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------FSRSGFRV 246
L +VP V ETYKF V+++ V +E D + S R+
Sbjct: 426 LVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGDQVTLANWQNNSSLRL 485
Query: 247 RNVLFDLTPWKFIRGIITELGIV 269
N+++D+TP + + +ITELG++
Sbjct: 486 LNLVYDVTPPELVDLVITELGMI 508
>emb|CAA53204.1| (X75451) translation initiation factor eIF-2B-delta [Oryctolagus
cuniculus]
Length = 527
Score = 92.1 bits (225), Expect = 7e-18
Identities = 70/270 (25%), Positives = 123/270 (44%), Gaps = 47/270 (17%)
Query: 40 DEALLEDAIRELRSRII---EVNPSMASLYNLARFMPITNNRELVKMRALEFLRRMDEAK 96
+E L D + +L+ I + P AS+YN +F+ N+E+ + + +R +EAK
Sbjct: 249 NEELSRDLVNKLKPYICFLTQCRPLSASMYNAIKFL----NKEITGVSST---KREEEAK 301
Query: 97 RELAS-------------------IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
EL + ++ I +GDVI+ + SS V I + A + F+
Sbjct: 302 AELQAAADRYVQEKIVLAAQAILRFASKKISNGDVILVYGCSSLVSRILQEAWSEGRKFR 361
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V++ +S P EG H+ L G+ + + + ++GA + +G V+++
Sbjct: 362 VVVVDSRPRLEGRHMLRFLVRAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSR 421
Query: 198 AGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------F 239
GT LAL +VP V ETYKF V+++ V +E D
Sbjct: 422 VGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCERGDHVALANWQ 481
Query: 240 SRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
S R+ N+++D+TP + + +ITELG++
Sbjct: 482 SHPSLRLLNLVYDVTPPELVDLVITELGMI 511
>sp|P41111|E2BD_RABIT TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|631601|pir||S42727 translation
initiation factor eIF-2 delta chain - rabbit
Length = 523
Score = 92.1 bits (225), Expect = 7e-18
Identities = 70/270 (25%), Positives = 123/270 (44%), Gaps = 47/270 (17%)
Query: 40 DEALLEDAIRELRSRII---EVNPSMASLYNLARFMPITNNRELVKMRALEFLRRMDEAK 96
+E L D + +L+ I + P AS+YN +F+ N+E+ + + +R +EAK
Sbjct: 245 NEELSRDLVNKLKPYICFLTQCRPLSASMYNAIKFL----NKEITGVSST---KREEEAK 297
Query: 97 RELAS-------------------IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
EL + ++ I +GDVI+ + SS V I + A + F+
Sbjct: 298 AELQAAADRYVQEKIVLAAQAILRFASKKISNGDVILVYGCSSLVSRILQEAWSEGRKFR 357
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V++ +S P EG H+ L G+ + + + ++GA + +G V+++
Sbjct: 358 VVVVDSRPRLEGRHMLRFLVRAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSR 417
Query: 198 AGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------F 239
GT LAL +VP V ETYKF V+++ V +E D
Sbjct: 418 VGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCERGDHVALANWQ 477
Query: 240 SRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
S R+ N+++D+TP + + +ITELG++
Sbjct: 478 SHPSLRLLNLVYDVTPPELVDLVITELGMI 507
>emb|CAB57260.1| (AJ011305) guanine nucleotide exchange factor, delta subunit [Homo
sapiens] >gi|6012057|emb|CAB57304.1| (AJ011307) guanine
nucleotide exchange factor [Homo sapiens]
Length = 522
Score = 89.7 bits (219), Expect = 3e-17
Identities = 69/270 (25%), Positives = 124/270 (45%), Gaps = 47/270 (17%)
Query: 40 DEALLEDAIRELR---SRIIEVNPSMASLYNLARFMPITNNRELVKMRALEFLRRMDEAK 96
+E L D + +L+ S + + P AS++N +F+ N+E+ + + +R +EAK
Sbjct: 244 NEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFL----NKEITSVGSS---KREEEAK 296
Query: 97 RELAS-------------------IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
EL + Q I +GDVI+ + SS V I + A + F+
Sbjct: 297 SELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFR 356
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V++ +S P EG H L + G+ + + + ++GA + +G V+++
Sbjct: 357 VVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSR 416
Query: 198 AGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------F 239
GT LAL +VP V ETYKF V+++ V +E D
Sbjct: 417 VGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQ 476
Query: 240 SRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
+ + R+ N+++D+TP + + +ITELG++
Sbjct: 477 NHASLRLLNLVYDVTPPELVDLVITELGMI 506
>emb|CAB57261.1| (AJ011306) guanine nucleotide exchange factor (long isoform) [Homo
sapiens]
Length = 543
Score = 89.7 bits (219), Expect = 3e-17
Identities = 69/270 (25%), Positives = 124/270 (45%), Gaps = 47/270 (17%)
Query: 40 DEALLEDAIRELR---SRIIEVNPSMASLYNLARFMPITNNRELVKMRALEFLRRMDEAK 96
+E L D + +L+ S + + P AS++N +F+ N+E+ + + +R +EAK
Sbjct: 265 NEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFL----NKEITSVGSS---KREEEAK 317
Query: 97 RELAS-------------------IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
EL + Q I +GDVI+ + SS V I + A + F+
Sbjct: 318 SELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFR 377
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V++ +S P EG H L + G+ + + + ++GA + +G V+++
Sbjct: 378 VVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSR 437
Query: 198 AGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------F 239
GT LAL +VP V ETYKF V+++ V +E D
Sbjct: 438 VGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQ 497
Query: 240 SRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
+ + R+ N+++D+TP + + +ITELG++
Sbjct: 498 NHASLRLLNLVYDVTPPELVDLVITELGMI 527
>emb|CAB57305.1| (AJ011307) guanine nucleotide exchange factor [Homo sapiens]
Length = 542
Score = 89.7 bits (219), Expect = 3e-17
Identities = 69/270 (25%), Positives = 124/270 (45%), Gaps = 47/270 (17%)
Query: 40 DEALLEDAIRELR---SRIIEVNPSMASLYNLARFMPITNNRELVKMRALEFLRRMDEAK 96
+E L D + +L+ S + + P AS++N +F+ N+E+ + + +R +EAK
Sbjct: 264 NEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFL----NKEITSVGSS---KREEEAK 316
Query: 97 RELAS-------------------IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
EL + Q I +GDVI+ + SS V I + A + F+
Sbjct: 317 SELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFR 376
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V++ +S P EG H L + G+ + + + ++GA + +G V+++
Sbjct: 377 VVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSR 436
Query: 198 AGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------F 239
GT LAL +VP V ETYKF V+++ V +E D
Sbjct: 437 VGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQ 496
Query: 240 SRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
+ + R+ N+++D+TP + + +ITELG++
Sbjct: 497 NHASLRLLNLVYDVTPPELVDLVITELGMI 526
>pir||T08757 probable translation initiation factor eIF-2B delta chain - human
(fragment) >gi|4884138|emb|CAB43277.1| (AL050109)
hypothetical protein [Homo sapiens]
Length = 394
Score = 89.7 bits (219), Expect = 3e-17
Identities = 69/270 (25%), Positives = 124/270 (45%), Gaps = 47/270 (17%)
Query: 40 DEALLEDAIRELR---SRIIEVNPSMASLYNLARFMPITNNRELVKMRALEFLRRMDEAK 96
+E L D + +L+ S + + P AS++N +F+ N+E+ + + +R +EAK
Sbjct: 116 NEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFL----NKEITSVGSS---KREEEAK 168
Query: 97 RELAS-------------------IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
EL + Q I +GDVI+ + SS V I + A + F+
Sbjct: 169 SELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFR 228
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V++ +S P EG H L + G+ + + + ++GA + +G V+++
Sbjct: 229 VVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSR 288
Query: 198 AGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------F 239
GT LAL +VP V ETYKF V+++ V +E D
Sbjct: 289 VGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQ 348
Query: 240 SRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
+ + R+ N+++D+TP + + +ITELG++
Sbjct: 349 NHASLRLLNLVYDVTPPELVDLVITELGMI 378
>sp|O27900|E2B1_METTH PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447870|pir||E69117
translation initiation factor eIF-2B, alpha subunit -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2623009|gb|AAB86338.1| (AE000939) translation
initiation factor eIF-2B, alpha subunit
[Methanobacterium thermoautotrophicum]
Length = 311
Score = 88.5 bits (216), Expect = 7e-17
Identities = 78/267 (29%), Positives = 119/267 (44%), Gaps = 24/267 (8%)
Query: 27 KGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASLYNLARFMPITNNRELVKMRAL 86
+GA A + + A+ E A ++ E+ S + NL + E A+
Sbjct: 46 RGAPA-IGVTAAFGVAMAELAGEDVEVAAEEIKASRPTAVNLFWAVDRVMKSESPLDEAI 104
Query: 87 EFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSV--------LEIFKVAKDRRKSFKV 138
E R + GA LI+DGD ++TH + ++ L + + A D K V
Sbjct: 105 TMYMEDMETNRAIGRHGAGLIEDGDTVLTHCNAGALACVDYGTALGVIRAAWDDGKRLTV 164
Query: 139 IITESSPDYEGLHLAN-ELENLGIEFEVITDSQMGLFCRKATIS--MVGADMVTRDGFVV 195
I E+ P +G L+ E++ GI ++ITD G + I ++GAD + G V
Sbjct: 165 ICDETRPVGQGARLSVWEMQQEGIPVKLITDVAAGYLMQTGMIDKVIIGADRIAEGG-VA 223
Query: 196 NKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFSR----SGFRVR---- 247
NK G+ ++ALA +VPFYVAA F ++ + ERD + G R+
Sbjct: 224 NKIGSLMVALAAKRFNVPFYVAAPMSTFDTENSIYDIEIEERDPAEVLYYGGCRIAPQDT 283
Query: 248 ---NVLFDLTPWKFIRGIITELGIVIP 271
N FD+ P I GIITE GI+ P
Sbjct: 284 EAINPAFDIVPSDLISGIITEKGILDP 310
>sp|Q9UI10|E2BD_HUMAN TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|6563202|gb|AAF17195.1|AF112207_1
(AF112207) translation initiation factor eIF-2b delta
subunit [Homo sapiens]
Length = 523
Score = 88.2 bits (215), Expect = 1e-16
Identities = 69/270 (25%), Positives = 124/270 (45%), Gaps = 47/270 (17%)
Query: 40 DEALLEDAIRELR---SRIIEVNPSMASLYNLARFMPITNNRELVKMRALEFLRRMDEAK 96
+E L D + +L+ S + + P AS++N +F+ N+E+ + + +R +EAK
Sbjct: 245 NEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFL----NKEITSVGSS---KREEEAK 297
Query: 97 RELAS-------------------IGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFK 137
EL + Q I +GDVI+ + SS V I + A + F+
Sbjct: 298 SELRAAIDRYVQEKIVLAAQAISRFSYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFR 357
Query: 138 VIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNK 197
V++ +S P EG H L + G+ + + + ++GA + +G V+++
Sbjct: 358 VVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSR 417
Query: 198 AGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD------------------F 239
GT LAL +VP V ETYKF V+++ V +E D
Sbjct: 418 VGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQ 477
Query: 240 SRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
+ + R+ N+++D+TP + + +ITELG++
Sbjct: 478 NHALLRLLNLVYDVTPPELVDLVITELGMI 507
>sp|Q09924|E2BD_SCHPO PROBABLE TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2130308|pir||S62591
translation initiation factor eif-2b delta subunit -
fission yeast (Schizosaccharomyces pombe)
>gi|7493512|pir||T38138 translation initiation factor
eif-2b delta subunit - fission yeast
(Schizosaccharomyces pombe) >gi|1067222|emb|CAA91965.1|
(Z67999) translation initiation factor eif-2b delta
subunit [Schizosaccharomyces pombe]
Length = 467
Score = 87.4 bits (213), Expect = 2e-16
Identities = 62/250 (24%), Positives = 118/250 (46%), Gaps = 35/250 (14%)
Query: 55 IIEVNPSMASLYNLARFMPIT-----------NNRELVKMRALEFLR-RMDEAKRELASI 102
++ P S+ N RF+ + +EL+ + ++R R+ A + +
Sbjct: 201 LVSTRPLSISMGNAIRFLKLEISVLDIDLTDDEGKELLLEKIDSYIRDRIIIAGQVIVQA 260
Query: 103 GAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIE 162
+ I DGDVI+T+ SS+V ++ AK+ K F+V++ +S P++EG L GIE
Sbjct: 261 ATEKIQDGDVILTYLHSSTVNDVLIHAKNVGKKFRVVVVDSRPEFEGRVCLKLLTEHGIE 320
Query: 163 FEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYK 222
+ S + ++ T +G + +G + ++AGT L++L HES+VP E+YK
Sbjct: 321 CTYVMISALSYIMQEVTKIFLGGHAMLSNGALYSRAGTSLISLLGHESNVPVIACCESYK 380
Query: 223 FHPTVKSNEVVLHE------------RDF-----------SRSGFRVRNVLFDLTPWKFI 259
F ++ + +V +E DF S ++ ++ +D+TP + I
Sbjct: 381 FTERIQLDSLVYNELAPGDQLVNMGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTPPRLI 440
Query: 260 RGIITELGIV 269
+ E+G++
Sbjct: 441 TVCVCEMGLL 450
>gb|AAD52847.2|AF137288_1 (AF137288) putative translation initiation factor 2B beta subunit
[Nicotiana tabacum]
Length = 415
Score = 87.4 bits (213), Expect = 2e-16
Identities = 62/202 (30%), Positives = 95/202 (46%), Gaps = 21/202 (10%)
Query: 87 EFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPD 146
E ++ + ++A + I +VI+T S +V+E AK++++SF+V + E +P
Sbjct: 186 ELIQDIATCHEQIAEQAVEHIHHNEVILTLGNSRTVMEFLCAAKEKKRSFRVFVAEGAPR 245
Query: 147 YEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALA 206
Y+G LA EL G++ VITDS + + + +VGA V +G V+ G ++ALA
Sbjct: 246 YQGHALAKELVARGLQTTVITDSAIFAMISRVNMVVVGAHAVMANGGVIAPVGMNMVALA 305
Query: 207 CHESDVPFYVAAETYKFHPTVKSN-EVVLHE----------------RDFSRSG----FR 245
VPF V A T+K P N EV+L+E DF S
Sbjct: 306 AQRHAVPFVVLAGTHKLCPLYPHNPEVLLNELKSPAELLDFGEFSDCLDFGSSSGSPLLH 365
Query: 246 VRNVLFDLTPWKFIRGIITELG 267
V N FD P + IT+ G
Sbjct: 366 VVNPAFDYVPPNLVSLFITDTG 387
>gb|AAF20216.1|AC012395_3 (AC012395) putative translation initiation factor EIF-2B beta
subunit [Arabidopsis thaliana]
Length = 407
Score = 86.2 bits (210), Expect = 4e-16
Identities = 59/200 (29%), Positives = 95/200 (47%), Gaps = 19/200 (9%)
Query: 87 EFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPD 146
+ ++ + ++A + I +VI+T S +VLE AK++++SF+V + E +P
Sbjct: 186 QLIQEIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPR 245
Query: 147 YEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALA 206
Y+G LA EL G++ VITDS + + + ++GA V +G V+ G + ALA
Sbjct: 246 YQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVGVNMAALA 305
Query: 207 CHESDVPFYVAAETYKFHPTVKSN-EVVLHE-------RDFSR-----------SGFRVR 247
+ VPF V A ++K P N EV+L+E DF +V
Sbjct: 306 AQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCLDFGAGSGSLQVV 365
Query: 248 NVLFDLTPWKFIRGIITELG 267
N FD P + IT+ G
Sbjct: 366 NPTFDYVPPNLVSLFITDTG 385
>sp|O31662|E2B_BACSU PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447872|pir||C69863 translation
initiation factor IF-2B alpha chain homolog ykrS
[similarity] - Bacillus subtilis
>gi|2633726|emb|CAB13228.1| (Z99111) similar to
initiation factor eIF-2B (alpha subunit) [Bacillus
subtilis]
Length = 353
Score = 85.8 bits (209), Expect = 5e-16
Identities = 83/300 (27%), Positives = 133/300 (43%), Gaps = 42/300 (14%)
Query: 8 EILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEV----NPSMA 63
++ + + ++RGA + A A++++ D + E R R+ ++ N S
Sbjct: 40 DVFDAIVTLKVRGAPAIGITAAFGLALAAKDIET----DNVTEFRRRLEDIKQYLNSSRP 95
Query: 64 SLYNLA-----------RFMPITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDV 112
+ NL+ + + + + A++ +E R + QL GD
Sbjct: 96 TAINLSWALERLSHSVENAISVNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDR 155
Query: 113 IITHSFSSSV--------LEIFKVAKDRRKSFKVIITESSPDYEGLHL-ANELENLGIEF 163
I+T + S+ L F +AK + + E+ P +G L A EL GI+
Sbjct: 156 IMTICNAGSIATSRYGTALAPFYLAKQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDV 215
Query: 164 EVITDSQMGLFCRKATIS--MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETY 221
+ITDS ++ IS +VGAD + ++G NK GTY LA+ + D+PF+VAA
Sbjct: 216 TLITDSMAAHTMKEKQISAVIVGADRIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLS 275
Query: 222 KFHPTVKSN-EVVLHERDFSR----SGFR-------VRNVLFDLTPWKFIRGIITELGIV 269
F VK ++ + ERD SG R V N FD+TP I GIITE GI+
Sbjct: 276 TFDTKVKCGADIPIEERDPEEVRQISGVRTAPSNVPVFNPAFDITPHDLISGIITEKGIM 335
>gb|AAF46995.1| (AE003460) CG10315 gene product [Drosophila melanogaster]
Length = 626
Score = 85.8 bits (209), Expect = 5e-16
Identities = 57/222 (25%), Positives = 111/222 (49%), Gaps = 17/222 (7%)
Query: 65 LYNLARFMPITNNRELVKMRALEFLR-RMDEAKRELASIGAQLIDDGDVIITHSFSSSVL 123
L L +P T ++EL+ ++ ++ +A + ++ + I DGDV++T + SS +
Sbjct: 388 LTQLPADVPETESKELLVHFIDTYIENQIGKAAQAISGFLQEKITDGDVLLTFACSSLIQ 447
Query: 124 EIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMV 183
I + AK R+ +F+VI+ +S P EG L L GI + + +G +AT ++
Sbjct: 448 FICEEAKRRQVAFRVIVVDSRPGCEGQELLRRLHATGIPCTYVLINAVGYVMAEATKVLL 507
Query: 184 GADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERD----- 238
GA + +G+V+ + GT +AL + +VP V ET+KF +++ +V +E
Sbjct: 508 GAHALLANGYVMARTGTAQVALVANAHNVPVLVCCETHKFSERFQTDAIVYNELSDPNQL 567
Query: 239 -----------FSRSGFRVRNVLFDLTPWKFIRGIITELGIV 269
++ N+ +D+TP + + ++TE+ I+
Sbjct: 568 VRGDKCQLSNWAAKGKLLPLNLSYDITPPELVSAVVTEVAIL 609
>gb|AAF02141.1|AC009853_1 (AC009853) putative translation initiation factor EIF-2B beta
subunit, 3' partial [Arabidopsis thaliana]
Length = 349
Score = 85.0 bits (207), Expect = 9e-16
Identities = 49/151 (32%), Positives = 82/151 (53%), Gaps = 1/151 (0%)
Query: 87 EFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPD 146
+ ++ + ++A + I +VI+T S +VLE AK++++SF+V + E +P
Sbjct: 186 QLIQEIAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPR 245
Query: 147 YEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALA 206
Y+G LA EL G++ VITDS + + + ++GA V +G V+ G + ALA
Sbjct: 246 YQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVGVNMAALA 305
Query: 207 CHESDVPFYVAAETYKFHPTVKSN-EVVLHE 236
+ VPF V A ++K P N EV+L+E
Sbjct: 306 AQKHAVPFVVLAGSHKLCPLYPHNPEVLLNE 336
>dbj|BAB10154.1| (AB005231) contains similarity to guanine nucleotide exchange
factor~gene_id:MBB18.19 [Arabidopsis thaliana]
Length = 642
Score = 82.7 bits (201), Expect = 4e-15
Identities = 69/251 (27%), Positives = 109/251 (42%), Gaps = 32/251 (12%)
Query: 53 SRIIEVNPSMASLYNLARF-------MPITNNRELVKMRALEFLRRMDEAKRELASI--- 102
S +IE P S+ N RF +PIT + K + R K +A
Sbjct: 375 SFLIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKATLQSDIERFINEKIIVADTVIV 434
Query: 103 --GAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLG 160
I DGDV++T+ ++V + A + RK F+V++ +S P EG L L G
Sbjct: 435 KHAVTKIRDGDVLLTYGSPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIKRG 494
Query: 161 IEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAET 220
I + + + T +GA V +G V ++ GT +A+ + VP V E
Sbjct: 495 INCTYTHINAISYIMHEVTKVFLGASSVLSNGTVYSRVGTACVAMVANAFRVPVLVCCEA 554
Query: 221 YKFHPTVK-----SNEV-------VLHERD--------FSRSGFRVRNVLFDLTPWKFIR 260
YKFH V+ SNE+ +H R+ + + + N+++D TP +I
Sbjct: 555 YKFHERVQLDSICSNELGDPNAISEVHGREDINYLDGLTNNANLQFLNLMYDATPSDYIS 614
Query: 261 GIITELGIVIP 271
IIT+ G+V P
Sbjct: 615 MIITDYGMVPP 625
>sp|P34604|E2BA_CAEEL PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|482164|pir||S40927
hypothetical protein ZK1098.4 - Caenorhabditis elegans
>gi|3881483|emb|CAA80132.1| (Z22176) similar to GCN3
(yeast transcription activator), contains similarity to
Pfam domain: PF01008 (Initiation factor 2 subunit
family), Score=413.9, E-value=5e-121, N=1
[Caenorhabditis elegans]
Length = 305
Score = 82.3 bits (200), Expect = 6e-15
Identities = 57/199 (28%), Positives = 92/199 (45%), Gaps = 6/199 (3%)
Query: 79 ELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKV 138
+L + RA F++ + ++ +++ I+THS+S VLE A V
Sbjct: 95 DLYRQRARSFIKNVRGSRAKISKCARLFFTHHMNILTHSYSKVVLETILDAHKSGYHLHV 154
Query: 139 IITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKA 198
+TES PD G + EL+ G+ ++ DS +G + +VGA+ V G ++NK
Sbjct: 155 WVTESQPDASGKLVFEELKKNGVPTTLVLDSCVGYVMERIQAVLVGAEGVMETGGIINKI 214
Query: 199 GTYLLALACHESDVPFYVAAETYKFHPTVKSNEVVLHERDFSRSGFRVRNVL------FD 252
GT + + VP YV AET KF N+ + + R+ RN L D
Sbjct: 215 GTVNVCIIAKSRHVPVYVCAETIKFVREFPLNQADIPQEFKYRTSVIERNNLELEHPDVD 274
Query: 253 LTPWKFIRGIITELGIVIP 271
T +F+ IIT++G + P
Sbjct: 275 YTAPEFLTLIITDVGAMKP 293
>gb|AAG19758.1| (AE005061) translation initiation factor eIF-2B subunit delta;
Eif2bd [Halobacterium sp. NRC-1]
Length = 288
Score = 81.1 bits (197), Expect = 1e-14
Identities = 71/281 (25%), Positives = 122/281 (43%), Gaps = 26/281 (9%)
Query: 8 EILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELR---SRIIEVNPSMAS 64
E E++ R ++ +A K A A AL + D + +R L + + NPS AS
Sbjct: 4 ETAEEIREMRTHSSSVVAVKAARALEALLDS-DYPTVAAYVRALERNSTALRRANPSHAS 62
Query: 65 LYNLARFM----------PITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVII 114
L R + I +E+ + + R++ K + A+ A+ I DG ++
Sbjct: 63 LVTTQREVVSRVRDADPASIPEAQEVTAAAIDDVVARVNMGKHDAAAATAEHITDGQTLL 122
Query: 115 THSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLG-IEFEVITDSQMGL 173
TH +SS+VLE ++A V +TE+ P + G L L ++ +I D G
Sbjct: 123 THDYSSTVLEAIELAAQDGAHLDVFVTEARPRHLGRKTVRALAQLDRVDPTLIVDGAAGH 182
Query: 174 FCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVA-------AETYKFHPT 226
++G D + D + N+ GTY + A ++ P VA A+ ++F
Sbjct: 183 VLDDCDRVLLGMDCIVADTY-YNRVGTYPIVAAAADAGTPVTVAGSAAKLVADGFQFTND 241
Query: 227 VKSNEVVLHERDFSRSGFRVRNVLFDLTPWKFIRGIITELG 267
+ V+ E +GF V N +D TP + + ++TE G
Sbjct: 242 FRDTSEVIRE---PPAGFTVENPAYDATPTRLLDAVVTEDG 279
>gb|AAF69705.1|AC016041_10 (AC016041) F27J15.24 [Arabidopsis thaliana]
>gi|11094807|gb|AAG29736.1|AC084414_4 (AC084414) guanine
nucleotide exchange factor eIF-2B delta subunit,
putative [Arabidopsis thaliana]
Length = 756
Score = 80.0 bits (194), Expect = 3e-14
Identities = 68/279 (24%), Positives = 120/279 (42%), Gaps = 40/279 (14%)
Query: 30 EAFLALAEELDEALLEDAIRELRSRI-------IEVNPSMASLYNLARFM-------PIT 75
+AF E+ ++D +L ++I IE P S+ N RF+ P+
Sbjct: 453 QAFQEAIEDYSTPPMKDLTMDLTAKINGYVSFLIECRPLSMSMGNAIRFLKNQIRKLPVN 512
Query: 76 NNRELVKMRALEFLRR-MDE----AKRELASIGAQLIDDGDVIITHSFSSSVLEIFKVAK 130
+ K + R +DE A + + I DG+V++T+ FS V I A
Sbjct: 513 LSESEAKSSLCSDIGRFIDEKTIIADKAIVQHAVTKIRDGEVLLTYGFSCVVEMILLYAH 572
Query: 131 DRRKSFKVIITESSPDYEGLHLANELENLGIEFEVITDSQMGLFCRKATISMVGADMVTR 190
+ K F+V+I +S P+ EG L L G++ + + R+AT +GA +
Sbjct: 573 EIGKKFRVVIVDSRPNLEGQKLLRRLVTRGLDCTYTHINAISYIMREATRVFLGASSIYS 632
Query: 191 DGFVVNKAGTYLLALACHESDVPFYVAAETYKFH-----PTVKSNEV------------- 232
+G + + GT +A+ + VP V E YKFH ++ SNE+
Sbjct: 633 NGTLYARVGTSCIAMVANAFSVPVIVCCEAYKFHERVLLDSICSNELGDPDAVANIPSFR 692
Query: 233 --VLHERDF-SRSGFRVRNVLFDLTPWKFIRGIITELGI 268
H + + + N+++D TP ++I I+++ G+
Sbjct: 693 TNAKHSKTMDNNKNLQFLNLMYDSTPSEYISMIVSDYGM 731
>pir||T00687 hypothetical protein F6E13.20 - Arabidopsis thaliana
>gi|3212863|gb|AAC23414.1| (AC004005) putative
translation initiation factor eIF-2B delta subunit
[Arabidopsis thaliana]
Length = 307
Score = 76.9 bits (186), Expect = 2e-13
Identities = 70/253 (27%), Positives = 110/253 (42%), Gaps = 35/253 (13%)
Query: 53 SRIIEVNPSMASLYNLARF-------MPITNNRELVKMRALEFLRRMDEAK-----RELA 100
S +IE P S+ N RF +PIT + K + R K +
Sbjct: 33 SFLIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKASLHSDIERFINEKIIGADTVIV 92
Query: 101 SIGAQLIDDGDVIITHSFSSSVLEIFKV-AKDRRKSFKVIITESSPDYEGLHLANELENL 159
I DGDV++T+ SS+V+E+ V A + K F+V + +S P +G L L
Sbjct: 93 EHAVTKIRDGDVLLTYG-SSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIKR 151
Query: 160 GIEFEVITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAE 219
GI + + + T +GA V +G V ++ GT +A+ + VP V E
Sbjct: 152 GINCTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVANAFRVPVLVCCE 211
Query: 220 TYKFHPTVK------SNEV-------VLHERD--------FSRSGFRVRNVLFDLTPWKF 258
YKFH V+ SNE+ +H R+ + + + N+++D TP +
Sbjct: 212 AYKFHEKVQLDYSICSNELGDPNAISKVHGREDINYLNGLTNNAKLKFLNLMYDATPSDY 271
Query: 259 IRGIITELGIVIP 271
I IIT+ G+V P
Sbjct: 272 ISMIITDYGMVPP 284
>sp|O58433|E2B1_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447867|pir||G71116
probable translation initiation factor eIF-2 beta chain
- Pyrococcus horikoshii >gi|3257110|dbj|BAA29793.1|
(AP000003) 364aa long hypothetical translation
initiation factor eIF-2 beta chain [Pyrococcus
horikoshii]
Length = 364
Score = 76.5 bits (185), Expect = 3e-13
Identities = 62/222 (27%), Positives = 106/222 (46%), Gaps = 23/222 (10%)
Query: 73 PITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIF-----K 127
P+ + L+ A + EA + GA+++ +G+ I+TH + S+ +
Sbjct: 124 PLDEIKRLIVQEAYKIADEDVEANLRMGHYGAEVLPEGN-ILTHCNAGSLATVHLGTVGS 182
Query: 128 VAKDRRK--SFKVI-ITESSPDYEGLHL-ANELENLGIEFEVITDSQMGLFCRKATIS-- 181
V + K S K++ + E+ P +G L A E G+ ++I D+ ++ +
Sbjct: 183 VVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGFVDAI 242
Query: 182 MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKS-NEVVLHERD-- 238
+VGAD + +G NK GTY+LA+ E +PF+ A +KS ++ + ER
Sbjct: 243 IVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMELKSGKDIPIEERSPE 302
Query: 239 --FSRSGFR------VRNVLFDLTPWKFIRGIITELGIVIPP 272
+ G R V N FD+TP K++ GIIT+ G+V PP
Sbjct: 303 EVLTCGGCRIAPDVPVYNPAFDVTPHKYLTGIITDRGVVWPP 344
>sp|Q9UZ16|E2B1_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447875|pir||A75044
translation initiation factor aif-2bi PAB2444 -
Pyrococcus abyssi (strain Orsay)
>gi|5458759|emb|CAB50246.1| (AJ248287) translation
initiation factor aIF-2BI [Pyrococcus abyssi]
Length = 356
Score = 73.7 bits (178), Expect = 2e-12
Identities = 61/222 (27%), Positives = 106/222 (47%), Gaps = 23/222 (10%)
Query: 73 PITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIF-----K 127
P+ + L+ A + EA + GA+++ +G+ ++TH + S+ +
Sbjct: 124 PLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGA 182
Query: 128 VAKDRRK--SFKVI-ITESSPDYEGLHL-ANELENLGIEFEVITDSQMGLFCRKATIS-- 181
V + K S K++ + E+ P +G L A E G+ ++I D+ ++ +
Sbjct: 183 VVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAI 242
Query: 182 MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKS-NEVVLHERD-- 238
+VGAD + +G NK GTY+LA+ E +PF+ A ++KS E+ + ER
Sbjct: 243 IVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPE 302
Query: 239 --FSRSGFR------VRNVLFDLTPWKFIRGIITELGIVIPP 272
+ G R V N FD+TP K++ GIIT+ +V PP
Sbjct: 303 EVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPP 344
>sp|Q9PAG5|E2B_XYLFA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|11279300|pir||A82544 translation
initiation factor IF-2B alpha subunit-related XF2553
[imported] - Xylella fastidiosa (strain 9a5c)
>gi|9107764|gb|AAF85350.1|AE004062_5 (AE004062)
initiation factor eIF-2B, alpha subunit-related [Xylella
fastidiosa]
Length = 354
Score = 71.4 bits (172), Expect = 1e-11
Identities = 69/297 (23%), Positives = 120/297 (40%), Gaps = 32/297 (10%)
Query: 8 EILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSRIIEVNPSMASLYN 67
++ ++A +RGA + + A E++ +A+R+L + ++ + + N
Sbjct: 47 DVTQAIRALIVRGAPAIGIVAGWGAVLAAREIEAVDGIEALRKLEPALQRLHAARPTAVN 106
Query: 68 LARFMPITNN---------RELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSF 118
LA + R+ + A R A R + + GA LI G ++TH
Sbjct: 107 LAWVLARMRRTLSAAHADWRQAMAREAESIAREDLTANRCMGAYGAALIPIGSGVLTHCN 166
Query: 119 SSSV--------LEIFKVAKDRRKSFKVIITESSPDYEGLHLAN-ELENLGIEFEVITDS 169
+ S+ L + + + + +V E+ P +G L EL+ GI+ +I DS
Sbjct: 167 TGSLATAGFGTALGVIRAGIAQGRIARVFAGETRPWLQGARLTVWELQQDGIDVTLIADS 226
Query: 170 QMGLFCRKATIS--MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTV 227
+ + +VGAD + +G NK GTY LA+ V F V A
Sbjct: 227 AAAHLMKSGQVQWVIVGADRICANGDAANKIGTYQLAITARHHGVKFMVVASAATVDMDT 286
Query: 228 KSNEVV-LHERDFSR-----------SGFRVRNVLFDLTPWKFIRGIITELGIVIPP 272
+ E + + +RD G N +FD+TP I I+TE G++ P
Sbjct: 287 TAGEAIEIEQRDPEELLGVSGVRTVAEGIAAWNPVFDVTPGALIDAIVTERGVIQSP 343
>gb|AAF57154.1| (AE003778) CG11334 gene product [alt 1] [Drosophila melanogaster]
>gi|7302053|gb|AAF57155.1| (AE003778) CG11334 gene
product [alt 2] [Drosophila melanogaster]
Length = 364
Score = 71.0 bits (171), Expect = 1e-11
Identities = 72/269 (26%), Positives = 115/269 (41%), Gaps = 42/269 (15%)
Query: 29 AEAFLALAEEL--DEALLEDAIRELRSRIIEVNPSMASLYNLARFMPITNNRELVKMRAL 86
A+ + LA EL DEA+ + +++ R ++ +M I +NR + A
Sbjct: 99 ADELITLANELYKDEAI---DVTQMKHRFLDATEAMLK-------KDIADNRAIGANGAQ 148
Query: 87 EFLRRMDEAKRELASIGAQLIDDGDV-IITHSFSSSV--------LEIFKVAKDRRKSFK 137
L+ + +A + A G V ++TH + S+ L + + + K
Sbjct: 149 AILQGVAKAGKAPAG------STGSVRVLTHCNTGSLATAGYGTALGVVRQLAELGKLEH 202
Query: 138 VIITESSPDYEGLHL-ANELENLGIEFEVITDSQMGLFCRKATIS--MVGADMVTRDGFV 194
V TE+ P +G L A EL + ++ DS + R ++ +VGAD V +G
Sbjct: 203 VYCTETRPYNQGARLTAYELVHEKFPATLVLDSMVAALLRAKNVAAVVVGADRVASNGDT 262
Query: 195 VNKAGTYLLALACHESDVPFYVAAETYKFHPTVKSNE-VVLHERD-----------FSRS 242
NK GTY +A+ DVPFYVAA + + +++ ER +
Sbjct: 263 ANKIGTYQIAVVAKHHDVPFYVAAPLTSIDLAIPGGDHIIIEERPDREMTHVGEHRIAAP 322
Query: 243 GFRVRNVLFDLTPWKFIRGIITELGIVIP 271
G N FD+TP I GIITE G+ P
Sbjct: 323 GINCWNPAFDVTPASLITGIITERGVFKP 351
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.323 0.138 0.389
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89974877
Number of Sequences: 2977
Number of extensions: 3339457
Number of successful extensions: 9678
Number of sequences better than 1.0e-10: 49
Number of HSP's better than 0.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 9589
Number of HSP's gapped (non-prelim): 53
length of query: 276
length of database: 189,106,746
effective HSP length: 57
effective length of query: 219
effective length of database: 154,992,987
effective search space: 33943464153
effective search space used: 33943464153
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 164 (68.3 bits)