BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1316 (PAB1316) DE:2-HALOALKANOIC ACID DEHALOGENASE (EC
3.8.1.2) RELATED PROTEIN
(229 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75005 probable haloacid dehalogenase (EC 3.8.1.-) PAB1316 ... 469 e-131
pir||D71157 probable haloacid dehalogenase (EC 3.8.1.-) PH0459 [... 403 e-111
pir||D64479 hypothetical protein MJ1437 - Methanococcus jannasch... 95 8e-19
emb|CAB57577.1| (Y18930) hypothetical protein [Sulfolobus solfat... 84 1e-15
gb|AAK41024.1| 2-haloalkanoic acid dehalogenase, putative [Sulfo... 84 1e-15
pir||H71208 hypothetical protein PH1936 - Pyrococcus horikoshii ... 84 2e-15
dbj|BAB18787.1| (AB048605) hypothetical protein [Thermus thermop... 78 9e-14
pir||B69983 conserved hypothetical protein ysaA - Bacillus subti... 77 2e-13
gb|AAK42086.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solf... 76 3e-13
pir||A75022 hypothetical protein PAB1224 - Pyrococcus abyssi (st... 76 3e-13
pir||F69125 conserved hypothetical protein MTH209 - Methanobacte... 75 8e-13
pir||E75169 hypothetical protein PAB2019 - Pyrococcus abyssi (st... 73 2e-12
gb|AAK42335.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solf... 72 7e-12
pir||S74066 hypothetical protein c0135 - Sulfolobus solfataricus... 72 7e-12
gb|AAK42215.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solf... 71 9e-12
pir||A75103 phosphoglycolate phosphatase PAB1576 - Pyrococcus ab... 69 4e-11
pir||G75522 hydrolase, CbbY/CbbZ/GpH/YieH family - Deinococcus r... 68 8e-11
pir||B82987 probable hydrolase PA5281 [imported] - Pseudomonas a... 68 8e-11
>pir||E75005 probable haloacid dehalogenase (EC 3.8.1.-) PAB1316 [similarity] -
Pyrococcus abyssi (strain Orsay)
>gi|5458997|emb|CAB50483.1| (AJ248288) 2-HALOALKANOIC
ACID DEHALOGENASE (EC 3.8.1.2 RELATED PROTEIN
[Pyrococcus abyssi]
Length = 229
Score = 469 bits (1193), Expect = e-131
Identities = 229/229 (100%), Positives = 229/229 (100%)
Query: 1 MIRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKP 60
MIRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKP
Sbjct: 1 MIRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKP 60
Query: 61 YRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEVLKELRQSYHVGM 120
YRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEVLKELRQSYHVGM
Sbjct: 61 YRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEVLKELRQSYHVGM 120
Query: 121 ITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVYVGD 180
ITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVYVGD
Sbjct: 121 ITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVYVGD 180
Query: 181 NPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIVKGQ 229
NPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIVKGQ
Sbjct: 181 NPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIVKGQ 229
>pir||D71157 probable haloacid dehalogenase (EC 3.8.1.-) PH0459 [similarity] -
Pyrococcus horikoshii >gi|3256862|dbj|BAA29545.1|
(AP000002) 232aa long hypothetical protein [Pyrococcus
horikoshii]
Length = 232
Score = 403 bits (1024), Expect = e-111
Identities = 189/226 (83%), Positives = 211/226 (92%)
Query: 1 MIRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKP 60
MIRAVFFDFVGTLLS EGEA THLKIMEEVL Y L+P+ +L+EYEKLTREAFS YAGKP
Sbjct: 1 MIRAVFFDFVGTLLSVEGEAKTHLKIMEEVLGDYPLNPKTLLDEYEKLTREAFSNYAGKP 60
Query: 61 YRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEVLKELRQSYHVGM 120
YRPIRDIEEE+MRK++E+YGFKYPENFWEIHL+MHQ YG+LYPEVVEVLK L+ YHVGM
Sbjct: 61 YRPIRDIEEEVMRKLAEKYGFKYPENFWEIHLRMHQRYGELYPEVVEVLKSLKGKYHVGM 120
Query: 121 ITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVYVGD 180
ITDSDTEYL AHL+ALG++DLFDSITTSEEAGFFKPHPRIFE+AL+KAGVKGE+AVYVGD
Sbjct: 121 ITDSDTEYLMAHLDALGIKDLFDSITTSEEAGFFKPHPRIFELALKKAGVKGEEAVYVGD 180
Query: 181 NPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIV 226
NPVKDCGG+KNLGM SILLDR GEK++ WD+CDF+VSDLREVIKIV
Sbjct: 181 NPVKDCGGSKNLGMTSILLDRKGEKREFWDKCDFIVSDLREVIKIV 226
>pir||D64479 hypothetical protein MJ1437 - Methanococcus jannaschii
>gi|1592085|gb|AAB99446.1| (U67584) L-2-haloalkanoic
acid dehalogenase isolog [Methanococcus jannaschii]
Length = 228
Score = 94.8 bits (232), Expect = 8e-19
Identities = 70/232 (30%), Positives = 119/232 (51%), Gaps = 14/232 (6%)
Query: 1 MIRAVFFDFVGTLL-SSEGEAVTHLKIMEEVLK-GYNLDPRKILEEYEKLTREAFSAYAG 58
MI+ + FD TL SSE + + ++ ++ G N+D + + K+ ++ S Y G
Sbjct: 1 MIKGILFDLDDTLYNSSEFVEIARREAVKSMIDAGLNIDFEEAMNILNKIIKDKGSNY-G 59
Query: 59 KPYRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEVLKELR-QSYH 117
K + +++++ + +Y K + + + YP ++ L EL+
Sbjct: 60 KHF-------DDLVKAVLGKYDPKIITTGIITYHNVKVALLRPYPHTIKTLMELKAMGLK 112
Query: 118 VGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVY 177
+G+ITD T L LG+ FD + TSEE G KPH F+ L++ G+K E+ VY
Sbjct: 113 LGVITDGLTIKQWEKLIRLGIHPFFDDVITSEEFGLGKPHLEFFKYGLKRMGLKAEETVY 172
Query: 178 VGDNPVKDCGGAKNLGMISILLDRTGEKKDLWD--RCDFVVSDLREVIKIVK 227
VGD KD AK LGMI++ + + G+ KD+ D D+ ++ L+E++ IVK
Sbjct: 173 VGDRVDKDIKPAKELGMITVRILK-GKYKDMEDDEYSDYTINSLQELVDIVK 223
>emb|CAB57577.1| (Y18930) hypothetical protein [Sulfolobus solfataricus]
Length = 242
Score = 84.3 bits (205), Expect = 1e-15
Identities = 75/247 (30%), Positives = 112/247 (44%), Gaps = 45/247 (18%)
Query: 2 IRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKPY 61
++AV FDF TL+ +A L + + + Y I E Y++
Sbjct: 11 VKAVLFDFDDTLVDFSSKAKEALDAVSKDIYTY------IKENYQQEIN----------I 54
Query: 62 RPIRDIEEEIMRKISEEYGFKYPENFWEIHLK----MHQEYGKLYP-------------- 103
IR + EE RK+ + G +WE LK +H + +LY
Sbjct: 55 NIIRKLVEEESRKLDSQ-GEYNRNKWWENILKSLNIVHIDKSQLYDWTSLYWSIASQTEP 113
Query: 104 -----EVVEVLKELRQSYHVGMITDSDTEYLN--AHLEALGVRDLFDSITTSEEAGFF-K 155
E++E L + Y +G++T+SD E N + L + D FD I + E G K
Sbjct: 114 YEDAKEIIEYLDS--KGYKLGLVTNSDGEGGNKSSRLRTFPLIDKFDLILIAGEGGIRPK 171
Query: 156 PHPRIFEIALRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFV 215
P+ F I+ K V+ V+VGD PVKDC AK MIS+L+DR G+ KD DFV
Sbjct: 172 PNLEPFIISCEKLSVEPSSCVFVGDEPVKDCLAAKKANMISVLIDREGKVKDAELYADFV 231
Query: 216 VSDLREV 222
+S L+++
Sbjct: 232 LSSLKQL 238
>gb|AAK41024.1| 2-haloalkanoic acid dehalogenase, putative [Sulfolobus
solfataricus]
Length = 242
Score = 84.3 bits (205), Expect = 1e-15
Identities = 75/247 (30%), Positives = 112/247 (44%), Gaps = 45/247 (18%)
Query: 2 IRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKPY 61
++AV FDF TL+ +A L + + + Y I E Y++
Sbjct: 11 VKAVLFDFDDTLVDFSSKAKEALDAVSKDIYTY------IKENYQQEIN----------I 54
Query: 62 RPIRDIEEEIMRKISEEYGFKYPENFWEIHLK----MHQEYGKLYP-------------- 103
IR + EE RK+ + G +WE LK +H + +LY
Sbjct: 55 NIIRKLVEEESRKLDSQ-GEYNRNKWWENILKSLNIVHIDKSQLYDWTSLYWSIASQTEP 113
Query: 104 -----EVVEVLKELRQSYHVGMITDSDTEYLN--AHLEALGVRDLFDSITTSEEAGFF-K 155
E++E L + Y +G++T+SD E N + L + D FD I + E G K
Sbjct: 114 YEDAKEIIEYLDS--KGYKLGLVTNSDGEGGNKSSRLRTFPLIDKFDLILIAGEGGIRPK 171
Query: 156 PHPRIFEIALRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFV 215
P+ F I+ K V+ V+VGD PVKDC AK MIS+L+DR G+ KD DFV
Sbjct: 172 PNLEPFIISCEKLSVEPSSCVFVGDEPVKDCLAAKKANMISVLIDREGKVKDAELYADFV 231
Query: 216 VSDLREV 222
+S L+++
Sbjct: 232 LSSLKQL 238
>pir||H71208 hypothetical protein PH1936 - Pyrococcus horikoshii
>gi|3258380|dbj|BAA31063.1| (AP000007) 217aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 217
Score = 83.9 bits (204), Expect = 2e-15
Identities = 71/242 (29%), Positives = 119/242 (48%), Gaps = 41/242 (16%)
Query: 1 MIRAVFFDFVGTLLSSEGEAVTHL-KIMEEVLKGYNL---DPRKI-LEEYEKLTREA--- 52
M++AV FD GTLLS + + L ++ +E+ N+ + R+I L E EK +
Sbjct: 1 MVKAVLFDIDGTLLSEKPLIMFILPQVYDELANKLNISRMEARRIFLSEIEKRKGKYEWH 60
Query: 53 ----FSAYAGKPYRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEV 108
F P++ E++ KYP +++P VVEV
Sbjct: 61 DWNFFFRLFSLPFK-------------YEDFIMKYPTKI------------EVFPGVVEV 95
Query: 109 LKELRQ-SYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRK 167
LK L++ Y +G++T S Y L+ G+ FD + T ++ G KP PRIF AL K
Sbjct: 96 LKYLKERGYRLGIVT-SGPRYQVLKLKVSGIYRYFDVVVTRDDVGSIKPEPRIFLAALEK 154
Query: 168 AGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIVK 227
+ +DAV VGD+ +D GAK +GM S+ ++ GE ++ DF +S + ++ ++++
Sbjct: 155 LKISPKDAVMVGDSLEQDILGAKGVGMKSVWINVGGENG--YNLPDFEISSILQLPEVIE 212
Query: 228 GQ 229
+
Sbjct: 213 DE 214
>dbj|BAB18787.1| (AB048605) hypothetical protein [Thermus thermophilus]
Length = 218
Score = 78.0 bits (189), Expect = 9e-14
Identities = 61/175 (34%), Positives = 86/175 (48%), Gaps = 11/175 (6%)
Query: 51 EAFSAYAGKPYRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGK--LYPEVVEV 108
EA +A+AG P+R E + R+ EE G PE E+ +E + LYPE
Sbjct: 46 EALAAWAG-PFR------ERVFREALEEAGGP-PERARELAEAFFRERRRYPLYPEAEAF 97
Query: 109 LKELRQ-SYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRK 167
L E R+ + ++T+ + L G+ F + S E G KP PR+F +AL
Sbjct: 98 LAEARRRGLALALLTNGVPDLQREKLVGAGLAHHFSLVLISGEVGIGKPDPRLFRMALCA 157
Query: 168 AGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREV 222
GV E+A VGDNP KD GA+ G+ ++ +DR +D D V DLREV
Sbjct: 158 FGVAPEEAAMVGDNPQKDVRGARLAGVRAVWVDRGLRPEDPEASPDLRVGDLREV 212
>pir||B69983 conserved hypothetical protein ysaA - Bacillus subtilis
>gi|1770000|emb|CAA99609.1| (Z75208) hypothetical
protein [Bacillus subtilis] >gi|2635359|emb|CAB14854.1|
(Z99118) similar to hypothetical proteins [Bacillus
subtilis]
Length = 260
Score = 76.9 bits (186), Expect = 2e-13
Identities = 71/263 (26%), Positives = 111/263 (41%), Gaps = 41/263 (15%)
Query: 2 IRAVFFDFVGTLLSSEGEA-VTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKP 60
++AVFFD TLL E T + + K Y L P + + RE + +Y P
Sbjct: 1 MKAVFFDLDDTLLWDEKSVRTTFAETCLQAEKKYGLAPEEFEAAVREAARELYMSYETYP 60
Query: 61 Y------RPIRDIEEEIMRKISEEYGFK--------YPENFWEIHLKM----HQEYGK-- 100
Y P + ISE GF+ Y N W LK YG+
Sbjct: 61 YTVMIGINPFEGLWSNFSEPISE--GFQKLNKIVPGYRRNAWTNGLKALGIDDPAYGEYL 118
Query: 101 -------------LYPEVVEVLKELRQSYHVGMITDSDTEYLNAHLEALGVRDL---FDS 144
+Y E VL +L+ Y + ++T+ D L GV +L F+
Sbjct: 119 GEFFAAERRKRPFVYDETFAVLDQLKGKYELLLLTNGDPSLQKEKLA--GVPELAPYFNE 176
Query: 145 ITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGE 204
I S G KP IFE L+ ++ +DA+ VGDN D GA G+ ++ ++RT +
Sbjct: 177 IVISGAFGKGKPDVSIFEHCLKLMNIEKDDAIMVGDNLNTDILGASRAGIKTVWINRTDK 236
Query: 205 KKDLWDRCDFVVSDLREVIKIVK 227
K + + D+++S L ++ I++
Sbjct: 237 KNETDVKPDYIISSLHDLFPILE 259
>gb|AAK42086.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solfataricus]
Length = 212
Score = 76.5 bits (185), Expect = 3e-13
Identities = 69/232 (29%), Positives = 108/232 (45%), Gaps = 30/232 (12%)
Query: 1 MIRAVFFDFVGTLLSSEGEAVTHLKIMEEVLK--GYNLDPRKILEEYEKLTREAFSAYAG 58
M RA+F DF TL+ + L+I +LK GY+++ RK+ Y K A A
Sbjct: 1 MYRAIFLDFGNTLVGFKPAFYEKLQI---ILKEHGYDVEIRKVFRAYVK-------AMAI 50
Query: 59 KPYRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGK----LYPEVVEVLKELRQ 114
Y DI+E + Y P + I+ M + +Y EV+E L+ +R
Sbjct: 51 NNYAQPTDIKEFL-------YNLSIPPDEKLINSIMSSDIRDGEAFVYDEVIEFLETIRS 103
Query: 115 SYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGED 174
+ ++ + + LE LG+ FD++ S E G KP+P+IF IA+ K G
Sbjct: 104 ANLKLILVSNSSPRTKKLLEELGLVKYFDNLVLSHEIGIVKPNPKIFAIAISKGGY---P 160
Query: 175 AVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIV 226
A+++GD D GA+ + ++LLDR D + V DLRE+I +
Sbjct: 161 ALHIGDIYEIDYIGARRSYLDAVLLDR----YDFYPEIKEKVRDLREIIPTI 208
>pir||A75022 hypothetical protein PAB1224 - Pyrococcus abyssi (strain Orsay)
>gi|5459129|emb|CAB50615.1| (AJ248288) hypothetical
protein [Pyrococcus abyssi]
Length = 216
Score = 76.5 bits (185), Expect = 3e-13
Identities = 66/236 (27%), Positives = 114/236 (47%), Gaps = 30/236 (12%)
Query: 1 MIRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKP 60
M+RA+ FD TL+S + L + E+L K L + RE F
Sbjct: 1 MVRAILFDLDETLISERPLVLFILPQVYEILA-------KRLNVSKSEAREIFLG----- 48
Query: 61 YRPIRDIEEEIMRKISEEYGFKY-------PENFWEIHLKMHQEYGKLYPEVVEVLKELR 113
E E MR E + + Y P F E+ L ++ L P V + L+ LR
Sbjct: 49 -------EIERMRGRYEWHDWNYFFRRFSLPFKFEELILSYPEKITVL-PGVRDTLEILR 100
Query: 114 QSYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGE 173
+ Y + ++T S Y L+ G+ D FD++ T ++ KP+P+IF AL + V+
Sbjct: 101 EKYRLAIVT-SGPRYQILKLKVSGLLDYFDAVITRDDVKAIKPNPKIFIAALERLKVEPN 159
Query: 174 DAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIVKGQ 229
AV VGD+ +D GAK LG+ ++ +++ G+ ++ DF +S + E++++++ +
Sbjct: 160 KAVMVGDSLEQDVLGAKALGIKTVWINQKGDNG--YNLPDFEISSISELLEVLEDE 213
>pir||F69125 conserved hypothetical protein MTH209 - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621255|gb|AAB84715.1| (AE000808) conserved protein
[Methanobacterium thermoautotrophicum]
Length = 226
Score = 74.9 bits (181), Expect = 8e-13
Identities = 64/201 (31%), Positives = 93/201 (45%), Gaps = 7/201 (3%)
Query: 1 MIRAVFFDFVGTLLSSEGEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKP 60
M++AVFFD TL + G A K V+ L + EE KL RE S
Sbjct: 1 MLKAVFFDIDDTLYDTSGFAKLARKAALNVMIDAGLPLTQ--EEAYKLLREIISEKGSNY 58
Query: 61 YRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEVLKELR-QSYHVG 119
R + + + EE H + + +P L +L+ + Y +G
Sbjct: 59 DRHFNVLTKTV---FGEEKPLLIALGMITYH-NVKFALLRPFPNTTSTLIDLKSKGYRLG 114
Query: 120 MITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVYVG 179
+I++ T L LG+ FD + TS+E GF KP+ RIFE ALR+ G K E +V VG
Sbjct: 115 VISNGITIKQWEKLIRLGIHHFFDEVVTSDEVGFEKPNIRIFEEALRRMGCKPERSVMVG 174
Query: 180 DNPVKDCGGAKNLGMISILLD 200
+ +D GA N GM +IL++
Sbjct: 175 NKFNEDILGATNAGMSAILVN 195
>pir||E75169 hypothetical protein PAB2019 - Pyrococcus abyssi (strain Orsay)
>gi|5457946|emb|CAB49436.1| (AJ248284) hypothetical
protein [Pyrococcus abyssi]
Length = 238
Score = 73.4 bits (177), Expect = 2e-12
Identities = 59/235 (25%), Positives = 110/235 (46%), Gaps = 14/235 (5%)
Query: 1 MIRAVFFDFVGTLLSSE--GEAVTHLKIMEEVLKGYNLDPRKILEEYEKLTREAFSAYAG 58
MI+ +FFD TL+ + E I + G +D E +L +E S +
Sbjct: 1 MIKVIFFDLDDTLVDTTKLAELARRNAIENMIRHGLPVDFETAYSELMELIKEYGSNF-- 58
Query: 59 KPYRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYGKLYPEVVEVLKELRQ-SYH 117
P+ + ++R++ Y K+ + Y + P +VL LR+ Y
Sbjct: 59 -PHH-----FDYLLRRLDLPYNPKWVSAGVIAYHNTKFAYLREVPGARKVLIRLRELGYR 112
Query: 118 VGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDAVY 177
+G+ITD + + L + D F+ + S+ G KPHP+IF+ AL+ V ++A+
Sbjct: 113 LGIITDGNPVKQWEKILRLEIDDFFEHVIISDFEGVKKPHPKIFKKALKAFNVDAQEALM 172
Query: 178 VGDNPVKDCGGAKNLGMISILL---DRTGEKKDLWDRCDFVVSDLREVIKIVKGQ 229
VGD D GAKN+GM ++ + E+ + + D+ + L++++K+++ +
Sbjct: 173 VGDRLYSDIYGAKNVGMKTVWFKYGKYSKEELEYREYADYEIEKLQDLLKVIENE 227
>gb|AAK42335.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solfataricus]
Length = 222
Score = 71.8 bits (173), Expect = 7e-12
Identities = 64/243 (26%), Positives = 110/243 (44%), Gaps = 56/243 (23%)
Query: 12 TLLSSEGEAVTHLK---------IMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKPYR 62
T+ GE + H K ++E+ GYN+D R++ + K+ GK +
Sbjct: 3 TIFVDLGETLVHFKPRYHENIAYALKEI--GYNVDERRVFKAVAKIL--------GKHHY 52
Query: 63 PIRDIEEEIMRKISEEYG----FKYPENFWEIHLKMHQ--------------EYGKLYPE 104
P S+EYG F + E F+E+++ + EY +LY +
Sbjct: 53 P------------SQEYGGLSAFDFRELFYELNIYPDEQLITRLNSKNLLSGEY-ELYDD 99
Query: 105 VVEVLKELRQSYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIA 164
+ L+E + ++ + T + +E LG++ FD I S + KPHP+IF A
Sbjct: 100 SITFLEEAKGLGFKLVLVSNATRSIYKIVEDLGIKKYFDGIVASCDLNIMKPHPKIFSYA 159
Query: 165 LRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIK 224
+ + D +++GD D GAK G+ +ILLDR G ++ + VS+L E ++
Sbjct: 160 ME---IAKSDGIHIGDIYEIDVIGAKRAGLEAILLDRLGFYPEIREN---KVSNLLEALE 213
Query: 225 IVK 227
+VK
Sbjct: 214 LVK 216
>pir||S74066 hypothetical protein c0135 - Sulfolobus solfataricus
>gi|1707765|emb|CAA69437.1| (Y08256) orf c01035
[Sulfolobus solfataricus]
Length = 222
Score = 71.8 bits (173), Expect = 7e-12
Identities = 64/243 (26%), Positives = 110/243 (44%), Gaps = 56/243 (23%)
Query: 12 TLLSSEGEAVTHLK---------IMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGKPYR 62
T+ GE + H K ++E+ GYN+D R++ + K+ GK +
Sbjct: 3 TIFVDLGETLVHFKPRYHENIAYALKEI--GYNVDERRVFKAVAKIL--------GKHHY 52
Query: 63 PIRDIEEEIMRKISEEYG----FKYPENFWEIHLKMHQ--------------EYGKLYPE 104
P S+EYG F + E F+E+++ + EY +LY +
Sbjct: 53 P------------SQEYGGLSAFDFRELFYELNIYPDEQLITRLNSKNLLSGEY-ELYDD 99
Query: 105 VVEVLKELRQSYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIA 164
+ L+E + ++ + T + +E LG++ FD I S + KPHP+IF A
Sbjct: 100 SITFLEEAKGLGFKLVLVSNATRSIYKIVEDLGIKKYFDGIVASCDLNIMKPHPKIFSYA 159
Query: 165 LRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIK 224
+ + D +++GD D GAK G+ +ILLDR G ++ + VS+L E ++
Sbjct: 160 ME---IAKSDGIHIGDIYEIDVIGAKRAGLEAILLDRLGFYPEIREN---KVSNLLEALE 213
Query: 225 IVK 227
+VK
Sbjct: 214 LVK 216
>gb|AAK42215.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solfataricus]
Length = 212
Score = 71.4 bits (172), Expect = 9e-12
Identities = 68/231 (29%), Positives = 108/231 (46%), Gaps = 28/231 (12%)
Query: 1 MIRAVFFDFVGTLLSSEGEAVTHLK-IMEEVLKGYNLDPRKILEEYEKLTREAFSAYAGK 59
M RA+F DF TL+ + L+ I+ E GY++D R++ Y K A A
Sbjct: 1 MYRAIFVDFGNTLVGFKPAFYEKLQTILRE--HGYDVDIRRVFRAYVK-------AMAVN 51
Query: 60 PYRPIRDIEEEIMRKISEEYGFKYPENFWEI-HLK---MHQEYGKLYPEVVEVLKELRQS 115
Y DI+E + Y P + I H++ + +Y +V+E L+ +R +
Sbjct: 52 NYSQPTDIKEFL-------YNLNIPPSDRLISHIRGSDIRDGEAFIYDDVMEFLETIRST 104
Query: 116 YHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRIFEIALRKAGVKGEDA 175
++ + + LE LG+ FD + S E G KP+P+IF IA+ K G A
Sbjct: 105 NTKLILLSNSSPRTKKLLEELGLVKYFDDLVLSHEIGIVKPNPKIFAIAILKGGY---PA 161
Query: 176 VYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSDLREVIKIV 226
+++GD D GA+ + +ILLDR D + V +LRE+I +V
Sbjct: 162 LHIGDIYEIDYVGARRSYVDAILLDR----YDFYPEIKEKVRNLREIIPMV 208
>pir||A75103 phosphoglycolate phosphatase PAB1576 - Pyrococcus abyssi (strain
Orsay) >gi|5458638|emb|CAB50126.1| (AJ248286)
PHOSPHOGLYCOLATE PHOSPHATASE [Pyrococcus abyssi]
Length = 231
Score = 69.1 bits (166), Expect = 4e-11
Identities = 57/191 (29%), Positives = 92/191 (47%), Gaps = 10/191 (5%)
Query: 40 KILEEYEKLTREAFSAYAGKPYRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYG 99
K E EKL + G R + DI E++ R+ +E K + F+ LK
Sbjct: 45 KRYERLEKLRDLHLLDFKGIFNRIMEDIYEDVDREEVKEI-LKLFDRFFFSSLK------ 97
Query: 100 KLYPEVVEVLKELRQ-SYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHP 158
LY + LKEL+ + ++TDS + + LE LG+ + FD++ S + G K P
Sbjct: 98 -LYEDAEYFLKELKNLKAKIVLVTDSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEP 156
Query: 159 RIFEIALRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSD 218
F++A ++ + E VGD D G K++G I+IL+ R K D VV+D
Sbjct: 157 YNFKLA-KRMFPREEIVFVVGDRDETDMKGGKSIGAITILVRRGYFKGRRVRYADHVVND 215
Query: 219 LREVIKIVKGQ 229
L E ++++K +
Sbjct: 216 LYEALEVIKNE 226
>pir||G75522 hydrolase, CbbY/CbbZ/GpH/YieH family - Deinococcus radiodurans
(strain R1) >gi|6458094|gb|AAF09992.1|AE001901_7
(AE001901) hydrolase, CbbY/CbbZ/GpH/YieH family
[Deinococcus radiodurans]
Length = 155
Score = 68.3 bits (164), Expect = 8e-11
Identities = 42/136 (30%), Positives = 71/136 (51%), Gaps = 3/136 (2%)
Query: 91 HLKMHQEYGKLYPEVVEVLKELRQ-SYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSE 149
H ++ + P EVL ELR+ ++G++T+ + L + DL D + SE
Sbjct: 21 HYDTALDHAQAMPHAAEVLTELRRRGVNIGVVTNGWEDAQTRCLAGCDLSDLADDVVISE 80
Query: 150 EAGFFKPHPRIFEIALRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLW 209
G KP P I+ +AL++ GV + +VGD+P D G + +GM + L TG +
Sbjct: 81 AVGLSKPDPCIYHLALKRLGVTTAHSWFVGDSPRNDVWGPQQVGMRAAYLP-TGHPLN-G 138
Query: 210 DRCDFVVSDLREVIKI 225
+R D ++DLR+V+ +
Sbjct: 139 ERPDVTLTDLRDVLNL 154
>pir||B82987 probable hydrolase PA5281 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9951594|gb|AAG08666.1|AE004940_10
(AE004940) probable hydrolase [Pseudomonas aeruginosa]
Length = 232
Score = 68.3 bits (164), Expect = 8e-11
Identities = 37/104 (35%), Positives = 59/104 (56%), Gaps = 5/104 (4%)
Query: 101 LYPEVVEVLKELRQSYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHPRI 160
L+PEV L+ L + +G++T+ NA + LG+ D F +EE G KP P
Sbjct: 108 LFPEVHPTLEILADRFTLGVLTNG-----NADVRRLGLADYFRFALCAEELGVGKPDPTP 162
Query: 161 FEIALRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGE 204
F AL++AGV+ A+++GD+P D GA+ GM +I + +G+
Sbjct: 163 FREALKRAGVEASAAIHIGDHPSDDIAGARRAGMRAIWFNPSGK 206
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.140 0.408
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86895867
Number of Sequences: 2977
Number of extensions: 3778636
Number of successful extensions: 10363
Number of sequences better than 1.0e-10: 18
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 10346
Number of HSP's gapped (non-prelim): 20
length of query: 229
length of database: 189,106,746
effective HSP length: 54
effective length of query: 175
effective length of database: 156,788,448
effective search space: 27437978400
effective search space used: 27437978400
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 163 (67.9 bits)