BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1320 (PAB1320) DE:THERMOSTABLE CARBOXYPEPTIDASE
(501 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75004 thermostable carboxypeptidase PAB1320 - Pyrococcus a... 1026 0.0
pir||C71158 probable thermostable carboxypeptidase - Pyrococcus ... 965 0.0
pir||E72561 probable thermostable carboxypeptidase APE1775 - Aer... 565 e-160
gb|AAK43208.1| Thermostable carboxypeptidase 1 [Sulfolobus solfa... 402 e-111
sp|P50848|YPWA_BACSU HYPOTHETICAL 58.2 KDA PROTEIN IN KDGT-XPT I... 357 2e-97
dbj|BAB05868.1| (AP001514) carboxypeptidase [Bacillus halodurans] 338 8e-92
sp|P42663|CTAQ_THEAQ THERMOSTABLE CARBOXYPEPTIDASE 1 (CARBOXYPEP... 325 6e-88
gb|AAG20654.1| (AE005134) probable carboxypeptidase; Cxp [Haloba... 316 3e-85
pir||G75276 carboxypeptidase - Deinococcus radiodurans (strain R... 298 7e-80
gb|AAA96610.1| (L77246) putative [Bacillus subtilis] 296 4e-79
pir||B82202 thermostable carboxypeptidase 1 VC1414 [imported] - ... 275 1e-72
gb|AAB38482.1| (L47838) putative [Bacillus subtilis] 177 2e-43
>pir||E75004 thermostable carboxypeptidase PAB1320 - Pyrococcus abyssi (strain
Orsay) >gi|5458989|emb|CAB50475.1| (AJ248288)
THERMOSTABLE CARBOXYPEPTIDASE [Pyrococcus abyssi]
Length = 501
Score = 1026 bits (2624), Expect = 0.0
Identities = 501/501 (100%), Positives = 501/501 (100%)
Query: 1 MEGQEVFKNETIKKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQ 60
MEGQEVFKNETIKKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQ
Sbjct: 1 MEGQEVFKNETIKKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQ 60
Query: 61 ELLLKPYFVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKA 120
ELLLKPYFVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKA
Sbjct: 61 ELLLKPYFVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKA 120
Query: 121 WEEAKAKDDFSKFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEV 180
WEEAKAKDDFSKFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEV
Sbjct: 121 WEEAKAKDDFSKFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEV 180
Query: 181 LEKKLRPLLDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAH 240
LEKKLRPLLDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAH
Sbjct: 181 LEKKLRPLLDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAH 240
Query: 241 PFTTEFGIRDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIH 300
PFTTEFGIRDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIH
Sbjct: 241 PFTTEFGIRDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIH 300
Query: 301 ESQSRFWENIIGRSREFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPDFIRTEADVVT 360
ESQSRFWENIIGRSREFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPDFIRTEADVVT
Sbjct: 301 ESQSRFWENIIGRSREFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPDFIRTEADVVT 360
Query: 361 YNFHIILRFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIG 420
YNFHIILRFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIG
Sbjct: 361 YNFHIILRFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIG 420
Query: 421 YFPTYSIGTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLR 480
YFPTYSIGTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLR
Sbjct: 421 YFPTYSIGTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLR 480
Query: 481 RAIGEEMDAEYFIRWIEERYL 501
RAIGEEMDAEYFIRWIEERYL
Sbjct: 481 RAIGEEMDAEYFIRWIEERYL 501
>pir||C71158 probable thermostable carboxypeptidase - Pyrococcus horikoshii
>gi|3256869|dbj|BAA29552.1| (AP000002) 515aa long
hypothetical thermostable carboxypeptidase [Pyrococcus
horikoshii]
Length = 515
Score = 965 bits (2468), Expect = 0.0
Identities = 460/498 (92%), Positives = 490/498 (98%)
Query: 4 QEVFKNETIKKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELL 63
+EVF+N+TIK+IL+KYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELL
Sbjct: 18 EEVFRNDTIKEILQKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELL 77
Query: 64 LKPYFVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEE 123
LKP FVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFL+EVSETTSKATKAWEE
Sbjct: 78 LKPEFVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLKEVSETTSKATKAWEE 137
Query: 124 AKAKDDFSKFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEK 183
AKAKDDFSKFEPWLDKII LAK+AAEYLGYEEEPYDALLDLYEEGLRTRDVVKMF+VLEK
Sbjct: 138 AKAKDDFSKFEPWLDKIISLAKRAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFDVLEK 197
Query: 184 KLRPLLDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFT 243
+LRPLL+KILEE KVPREH LEKEKYEREWME+VNLWVLEKFGYPLGTRARLDVSAHPFT
Sbjct: 198 ELRPLLEKILEEDKVPREHPLEKEKYEREWMEKVNLWVLEKFGYPLGTRARLDVSAHPFT 257
Query: 244 TEFGIRDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQ 303
TEFGIRDVRITTRYEGFDFRR LLST+HEFGHALYELQQDERFMFTPIAGGVSLGIHESQ
Sbjct: 258 TEFGIRDVRITTRYEGFDFRRALLSTIHEFGHALYELQQDERFMFTPIAGGVSLGIHESQ 317
Query: 304 SRFWENIIGRSREFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPDFIRTEADVVTYNF 363
SRFWENIIGRS+EFVE IYPVLKENLPF++KYTQEDVYLYFNMVRPDFIRTEADVVTYNF
Sbjct: 318 SRFWENIIGRSKEFVEFIYPVLKENLPFMEKYTQEDVYLYFNMVRPDFIRTEADVVTYNF 377
Query: 364 HIILRFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIGYFP 423
H++LRFKLER+MVSE+VKA DLPEMWN+EMERLLGIRPK+Y+EGILQDIHWAHGSIGYFP
Sbjct: 378 HVLLRFKLERMMVSEDVKAKDLPEMWNDEMERLLGIRPKTYREGILQDIHWAHGSIGYFP 437
Query: 424 TYSIGTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAI 483
TYSIGTILSAQLYYHIK+DIPDFEEKVAKGEFEPIK WLREKIHRWGSIYPPKELL++AI
Sbjct: 438 TYSIGTILSAQLYYHIKKDIPDFEEKVAKGEFEPIKSWLREKIHRWGSIYPPKELLKKAI 497
Query: 484 GEEMDAEYFIRWIEERYL 501
GE+++AEYF+RWI+ERYL
Sbjct: 498 GEDVNAEYFVRWIKERYL 515
>pir||E72561 probable thermostable carboxypeptidase APE1775 - Aeropyrum pernix
(strain K1) >gi|5105465|dbj|BAA80778.1| (AP000062) 538aa
long hypothetical thermostable carboxypeptidase
[Aeropyrum pernix]
Length = 538
Score = 565 bits (1441), Expect = e-160
Identities = 262/496 (52%), Positives = 361/496 (71%), Gaps = 1/496 (0%)
Query: 5 EVFKNETIKKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELLL 64
EVF+N +++++E YR +W++ HA S++GWD E MPK G+ ER++A+ +L VL + LLL
Sbjct: 41 EVFENSVVRELVEAYRPVWSVSHALSLMGWDSETYMPKGGVGERALARADLFVLRRRLLL 100
Query: 65 KPYFVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEA 124
+ + L+E+A+ E LNEYE GVVRVL R++RI +S P EF+ E ++ +AT AW A
Sbjct: 101 REELLRLLERAESQEGLNEYEAGVVRVLKREVRIARSLPEEFVFEFAKLREEATHAWRTA 160
Query: 125 KAKDDFSKFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKK 184
+ +DDF F+P+L++I+E A++ A+YLG+E PY+ALLDL+EEGL DV ++F+ +
Sbjct: 161 RERDDFGLFKPYLERIVEYARRMADYLGWEGHPYNALLDLHEEGLTVGDVDRIFDSIVPT 220
Query: 185 LRPLLDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFTT 244
LR LD++L G PR H LE +YE+ ME VN VL+ G+P R RLDVS HPFT
Sbjct: 221 LRRALDRVLSTGSYPRSHSLESVEYEQGVMEAVNREVLDLLGFPWD-RGRLDVSPHPFTI 279
Query: 245 EFGIRDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQS 304
GI DVRITTRYEG DFR TL + +HEFGHALYELQ DER +P+A G SLG+HESQS
Sbjct: 280 SMGIGDVRITTRYEGRDFRHTLFAVIHEFGHALYELQIDERLKASPLAEGASLGVHESQS 339
Query: 305 RFWENIIGRSREFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPDFIRTEADVVTYNFH 364
RFWEN++ RSR F+ L+ P+L++ LPF+ Y+ +DVYLYFN+VRP IR EAD +TYNFH
Sbjct: 340 RFWENVVARSRSFIPLVTPILRKRLPFLKDYSDDDVYLYFNIVRPGLIRVEADELTYNFH 399
Query: 365 IILRFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIGYFPT 424
I LR+ LE+ ++ +K DLPE+WN ME LLG+RPKSY++G+LQDIHW+HGSIGYFPT
Sbjct: 400 IYLRYTLEKGLIEGSIKVDDLPELWNSMMEDLLGVRPKSYRDGVLQDIHWSHGSIGYFPT 459
Query: 425 YSIGTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAIG 484
Y++GT+LSAQ+ I + V GE + ++ +LRE IHR+GS Y PKEL+ R +G
Sbjct: 460 YTLGTVLSAQIKAAIDGSLGGLYRLVENGELDRVRTFLRENIHRYGSTYAPKELVERGLG 519
Query: 485 EEMDAEYFIRWIEERY 500
E ++ EY+ ++I E++
Sbjct: 520 EPLNPEYYNQYITEKF 535
>gb|AAK43208.1| Thermostable carboxypeptidase 1 [Sulfolobus solfataricus]
Length = 467
Score = 402 bits (1022), Expect = e-111
Identities = 206/486 (42%), Positives = 301/486 (61%), Gaps = 26/486 (5%)
Query: 19 YRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELLLKPYFVELVEKAKGI 78
Y IWA+ HA S+L WD++ MP+ GI R A LS L ++LLL + + + +
Sbjct: 2 YEDIWAIEHAISLLDWDIQTYMPQSGIKARGEALARLSNLRRKLLLG-----IRGEIEKL 56
Query: 79 EDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEAKAKDDFSKFEPWLD 138
E N+ E+G+ RVL+R+ + + P E ++ TS+AT W AKAK DF+ F+P+L+
Sbjct: 57 EPKNDIEKGLKRVLEREYKYYDAVPEELDMKLHRITSEATVVWRNAKAKGDFNAFKPYLE 116
Query: 139 KIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKKLRPLLDKILEEGKV 198
+I+E+ ++ A LGY++ PY ALLD YEEG D ++F L L +L+KI + K
Sbjct: 117 QILEIKREIAHKLGYKDHPYSALLDRYEEGFTVTDAERVFNELLPGLSKILNKI--DDKF 174
Query: 199 PREHELEKEKYEREWMERVNLWVLEKFGYPL----GTRARLDVSAHPFTTEFGIRDVRIT 254
R++ E EKY+ M + +E Y + R R+DVS HPFT DVRIT
Sbjct: 175 TRKYHFEDEKYDVFQMSKT----IEAIAYEVLKMPKDRFRIDVSPHPFTVSMSRNDVRIT 230
Query: 255 TRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQSRFWENIIGRS 314
RYEG+DF+R L S VHE GHA+YELQ D ++P+A S+G+HESQSRFWEN++GRS
Sbjct: 231 VRYEGYDFKRVLYSLVHESGHAIYELQIDPSLEYSPLANAPSMGLHESQSRFWENVVGRS 290
Query: 315 REFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPDFIRTEADVVTYNFHIILRFKLERL 374
F++ IYP+L K + +DVY Y N V+ IR +AD VTYNFHI +R+++E+
Sbjct: 291 YGFIKTIYPLLNV------KDSIDDVYYYVNGVKRQPIRVDADEVTYNFHIAIRYEIEKR 344
Query: 375 MVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIGYFPTYSIGTILSAQ 434
+ ++A + P ++N+ M++ L IRPK+ EG+LQD+HW+ GS GYFPTY++G +++
Sbjct: 345 AIEGSLEASEFPSLFNDLMDKYLNIRPKNDGEGVLQDVHWSQGSFGYFPTYTLGNVIAGM 404
Query: 435 LYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAIGEEMDAEYFIR 494
+YYH+K E E IK WLRE+IH++GSIY PKEL R+ GE + +
Sbjct: 405 VYYHMKS-----ERGFDISNIEGIKNWLRERIHKYGSIYSPKELQMRSFGEAYNPSRLLD 459
Query: 495 WIEERY 500
++ E+Y
Sbjct: 460 YMREKY 465
>sp|P50848|YPWA_BACSU HYPOTHETICAL 58.2 KDA PROTEIN IN KDGT-XPT INTERGENIC REGION
>gi|7451077|pir||D69943 carboxypeptidase homolog ypwA -
Bacillus subtilis >gi|2634627|emb|CAB14125.1| (Z99115)
similar to carboxypeptidase [Bacillus subtilis]
Length = 501
Score = 357 bits (906), Expect = 2e-97
Identities = 185/490 (37%), Positives = 285/490 (57%), Gaps = 4/490 (0%)
Query: 13 KKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELLLKPYFVELV 72
K+ + +RI A +++ WD PK G +R+ + G+LS + EL+
Sbjct: 8 KEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFNIQTSDRMKELI 67
Query: 73 EKA-KGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEAKAKDDFS 131
+ + +DL+E + V + ++ K P +E SKA AWEEAK K DFS
Sbjct: 68 DVLYERFDDLSEDTKKAVELAKKEYEENKKIPEAEYKEYVILCSKAETAWEEAKGKSDFS 127
Query: 132 KFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKKLRPLLDK 191
F P+L+++IE K+ Y GY+E PYDALLDL+E G+ + + ++F L++ + PL+ +
Sbjct: 128 LFSPYLEQLIEFNKRFITYWGYQEHPYDALLDLFEPGVTVKVLDQLFAELKEAIIPLVKQ 187
Query: 192 ILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFTTEFGIRDV 251
+ G P + K + +E + ++L+ L++ GY RLD + HPF T DV
Sbjct: 188 VTASGNKPDTSFITKA-FPKEKQKELSLYFLQELGYDFDG-GRLDETVHPFATTLNRGDV 245
Query: 252 RITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQSRFWENII 311
R+TTRY+ DFR + T+HE GHA+YE DE T ++ G S+GIHESQS F+EN I
Sbjct: 246 RVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLFYENFI 305
Query: 312 GRSREFVELIYPVLKENLPFIDK-YTQEDVYLYFNMVRPDFIRTEADVVTYNFHIILRFK 370
GR++ F Y ++E P K + +D N +P FIR EAD +TY HII+R++
Sbjct: 306 GRNKHFWTPYYKKIQEASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHIIIRYE 365
Query: 371 LERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIGYFPTYSIGTI 430
+E+ + S EV DLP +WN++ + LGI P++ EGILQD+HWA G GYFP+Y++G +
Sbjct: 366 IEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTDAEGILQDVHWAGGDFGYFPSYALGYM 425
Query: 431 LSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAIGEEMDAE 490
+AQL + D+P+F+ + +GEF PIK WL EK+H G P ++++ A GEE++
Sbjct: 426 YAAQLKQKMLEDLPEFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGEELNVR 485
Query: 491 YFIRWIEERY 500
Y I ++ +Y
Sbjct: 486 YLIDYLSNKY 495
>dbj|BAB05868.1| (AP001514) carboxypeptidase [Bacillus halodurans]
Length = 505
Score = 338 bits (858), Expect = 8e-92
Identities = 181/493 (36%), Positives = 277/493 (55%), Gaps = 8/493 (1%)
Query: 13 KKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSV----LSQELLLKPYF 68
K L + ++I A S+L WD PK+GI++RS G LS +S + Y
Sbjct: 10 KDFLNQVKKIEDYKQALSLLAWDSRTGAPKKGIVQRSEVIGTLSSEVFQMSTSTEMGEYL 69
Query: 69 VELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEAKAKD 128
L E+ E L+E R V ++ P E +E +++ WEEAK K
Sbjct: 70 HALQEEGVQAE-LSEITRKTVEECAKEYERNVKIPKEEYKEYVVLQNQSESIWEEAKEKA 128
Query: 129 DFSKFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKKLRPL 188
DF F P L+K++E K+ Y GY++ PY+ LLD YE G+ + ++F L ++L PL
Sbjct: 129 DFETFRPNLEKLVEFKKRFIGYWGYDKHPYNTLLDDYEPGVFVDTIDEVFGTLREQLIPL 188
Query: 189 LDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFTTEFGI 248
+ + E P E ++ E++ + + ++ VL + GY RLD + HPF
Sbjct: 189 VKGVTESATQP-EADVLFERFPKANQQALSEAVLREIGYDFEA-GRLDETVHPFAIGINP 246
Query: 249 RDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQSRFWE 308
DVR+TT+Y DFR +L T+HE GHALYE + + TP+ G S+GIHESQS FWE
Sbjct: 247 NDVRVTTKYNERDFRVSLFGTIHEGGHALYEQNISKELIGTPLCTGTSMGIHESQSLFWE 306
Query: 309 NIIGRSREFVELIYPVLKENLPF-IDKYTQEDVYLYFNMVRPDFIRTEADVVTYNFHIIL 367
+G+ F + Y +LK++ D + +D Y N +P IR EAD +TY HIIL
Sbjct: 307 KFVGKHYGFWQRNYELLKQHASGQFDNVSLDDFYFAVNQAKPSLIRIEADELTYCLHIIL 366
Query: 368 RFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIGYFPTYSI 427
R++LE+ + ++ DLP +WNE+ME LG+RP EG+LQD+HW+ G+ GYFP+Y++
Sbjct: 367 RYELEKGLFENSIEVKDLPGLWNEKMEEYLGLRPSHDGEGVLQDVHWSFGAFGYFPSYAL 426
Query: 428 GTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAIGEEM 487
G I SAQL I +DIP F+ + G++ PI+ WL +K+H++G + P E+++ G +
Sbjct: 427 GYIYSAQLKEAILKDIPSFDRLIQSGDYAPIREWLTKKVHQYGKMKKPTEIIQDITGGGI 486
Query: 488 DAEYFIRWIEERY 500
D+ I+++ E+Y
Sbjct: 487 DSAPLIQYLNEKY 499
>sp|P42663|CTAQ_THEAQ THERMOSTABLE CARBOXYPEPTIDASE 1 (CARBOXYPEPTIDASE TAQ)
>gi|625662|pir||JC2294 carboxypeptidase (EC 3.4.-.-) Taq
- Thermus aquaticus (strain YT-1)
>gi|992957|dbj|BAA04551.1| (D17669) carboxypeptidase Taq
[Thermus aquaticus] >gi|1089937|prf||2018171A
carboxypeptidase Taq [Thermus aquaticus]
Length = 511
Score = 325 bits (825), Expect = 6e-88
Identities = 189/500 (37%), Positives = 263/500 (51%), Gaps = 14/500 (2%)
Query: 13 KKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELLLKPYFVELV 72
+ +LE R LG ++ WD +P++G R+ L+ L E P E +
Sbjct: 8 QNLLEFQRETAYLGSLGALAAWDQRTMIPRKGHGHRARQMAALARLLHERATDPRIGEWL 67
Query: 73 EKAKG---IEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEAKAKDD 129
EK +G +ED VR R ++ P E+++ S+ AWE + +DD
Sbjct: 68 EKVEGSSLVEDPLSDAAVNVRAWRRAYERARAIPERLAVELAQARSEGETAWEALRPRDD 127
Query: 130 FSKFEPWLDKIIELAKKAAEYL---GYEE------EPYDALLDLYEEGLRTRDVVKMFEV 180
+ F P+L ++ LAK+ AE L G + E YDALLD YE G R RD+ +F
Sbjct: 128 WQGFLPYLKRLFALAKEEAEILMAVGPDPLDPPYGELYDALLDGYEPGARARDLEPLFRE 187
Query: 181 LEKKLRPLLDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAH 240
L L+ LLD+IL G+ P L + Y +E L +L+ GY L RLD +AH
Sbjct: 188 LSSGLKGLLDRILGSGRRPDVGVLHRH-YPKEAQRAFALELLQACGYDLEA-GRLDPTAH 245
Query: 241 PFTTEFGIRDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIH 300
PF G DVRITTRY F + T+HE GHALYE E TP SLG+H
Sbjct: 246 PFEIAIGPGDVRITTRYYEDFFNAGIFGTLHEMGHALYEQGLPEAHWGTPRGEAASLGVH 305
Query: 301 ESQSRFWENIIGRSREFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPDFIRTEADVVT 360
ESQSR WEN++GRS F E +P KE + ED + N V P IR EAD VT
Sbjct: 306 ESQSRTWENLVGRSLGFWERFFPRAKEVFSSLADVRLEDFHFAVNAVEPSLIRVEADEVT 365
Query: 361 YNFHIILRFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIG 420
YN HI++R +LE + E+ DLPE W E+ LG+ P+ YK+G++QD+HW+ G G
Sbjct: 366 YNLHILVRLELELALFRGELFLEDLPEAWREKYRAYLGVAPRDYKDGVMQDVHWSGGMFG 425
Query: 421 YFPTYSIGTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLR 480
YFPTY++G + +AQ + + ++ E A+GEF P W R KIH GS + P+ L+
Sbjct: 426 YFPTYTLGNLYAAQFFAKAQEELGPLEPLFARGEFTPFLDWTRRKIHAEGSRFRPRALVE 485
Query: 481 RAIGEEMDAEYFIRWIEERY 500
R G A+ F+R++E +Y
Sbjct: 486 RVTGSPPGAQAFLRYLEAKY 505
>gb|AAG20654.1| (AE005134) probable carboxypeptidase; Cxp [Halobacterium sp. NRC-1]
Length = 502
Score = 316 bits (802), Expect = 3e-85
Identities = 172/487 (35%), Positives = 273/487 (55%), Gaps = 5/487 (1%)
Query: 15 ILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELLLKPYFVELVEK 74
+L+ Y I LG A +L WD EV MP+ G RS + +S + +LL + ++
Sbjct: 14 LLDHYETITGLGGAAGILSWDQEVMMPEGGTPARSTQKSAVSTVRHDLLTDQQVADWLDD 73
Query: 75 AKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEAKAKDDFSKFE 134
DL + + VVR + R+ P + + ++SE TS+A W++A+ +DDFS F
Sbjct: 74 LADA-DLTDEQAAVVREIRREHERAARVPSDLIADLSEKTSEAHPKWKQAREEDDFSIFA 132
Query: 135 PWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKKLRPLLDKILE 194
P L+ +I+L ++ A ++ + + Y+ L YE L ++ E L +L PL+ I
Sbjct: 133 PVLEDLIDLKREYANHIDPDADAYEVLFADYEPYLDLDTAERVLERLRDELVPLIADI-R 191
Query: 195 EGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFTTEFGIRDVRIT 254
+ V +++ + E + VL+ GY R+D + HPF++ D R+T
Sbjct: 192 DSDVTLADPFAGSQFDVDTQEDLARDVLDTLGYDW-EHGRIDTAPHPFSSGTPF-DARVT 249
Query: 255 TRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQSRFWENIIGRS 314
TR++ LLST+HEFGHA Y L TP+ L +HESQSR WEN IGRS
Sbjct: 250 TRFDPEKPVDALLSTIHEFGHARYTLGLPRAEYGTPLGESRDLTVHESQSRLWENHIGRS 309
Query: 315 REFVELIYPVLKENLPFIDKYTQEDVYLYFNMVRPD-FIRTEADVVTYNFHIILRFKLER 373
F E P +K P ++ T + + N + D IR EAD +TY+ HI++RF++ER
Sbjct: 310 PAFWEAFLPEMKTQFPQVEDATPQAAFEAANEIFEDNLIRVEADELTYHMHIVVRFEIER 369
Query: 374 LMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIGYFPTYSIGTILSA 433
++ ++ D+PE WN++ E LG+RP++ EG LQDIHW+ GSIGYFPTYS+G++L+A
Sbjct: 370 ELIEGDLDVADVPEAWNDKYEEYLGVRPETDAEGCLQDIHWSQGSIGYFPTYSLGSVLAA 429
Query: 434 QLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAIGEEMDAEYFI 493
Q++ ++ DI +E+V +G+F+ + WL +H+ G+ Y +L+R A GE A+YF+
Sbjct: 430 QIFAALEADIGPVDERVREGDFDVVGDWLESNVHQHGARYTTPDLVREATGEAFTADYFL 489
Query: 494 RWIEERY 500
+ E+Y
Sbjct: 490 DHVHEKY 496
>pir||G75276 carboxypeptidase - Deinococcus radiodurans (strain R1)
>gi|6460239|gb|AAF11966.1|AE002072_8 (AE002072)
carboxypeptidase [Deinococcus radiodurans]
Length = 503
Score = 298 bits (756), Expect = 7e-80
Identities = 171/490 (34%), Positives = 267/490 (53%), Gaps = 5/490 (1%)
Query: 13 KKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELLLKPYFVELV 72
+++ E ++ + G +++LGWD +P +R+ Q L+ L + +L+
Sbjct: 11 QQLTEHWQELADFGGIEALLGWDQSTFLPAGAAEDRARQQSLLAGLRHARATDAGYGKLL 70
Query: 73 EKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEAKAKDDFSK 132
+ A DL+ + +V+V + P EF+RE S ++ AW EA+ +DF +
Sbjct: 71 DAASSRSDLSPEQARMVQVARQDFEKATRIPAEFVREFSGHVGQSYSAWTEARPANDFGR 130
Query: 133 FEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKKLRPLLDKI 192
P+L+K ++L+ +AA Y +P D ++ +EG+ V ++F L L PL D +
Sbjct: 131 MVPYLEKTLDLSLQAASYFPEFGDPLDYYINESDEGMTAEQVGQVFAELRAALVPLADAV 190
Query: 193 LEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFTTEFGIRDVR 252
+ G PR L + + +E V+ +GY R R D++ HPF T G DVR
Sbjct: 191 IAAG-APRTDFLGRG-FAQERQLAFGERVIRDYGYDF-RRGRQDLTHHPFMTRLGGHDVR 247
Query: 253 ITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQSRFWENIIG 312
ITTR + D L ST+HE GHALYE D F+ TP+ GGVS G+HESQSR WEN++G
Sbjct: 248 ITTRVKEQDPTDALYSTLHEAGHALYEQGVDAAFLGTPLGGGVSAGVHESQSRLWENLVG 307
Query: 313 RSREFVELIYPVLKENLP-FIDKYTQEDVYLYFNMVRPDFIRTEADVVTYNFHIILRFKL 371
RSR F + ++ P + T+E++Y N V IRT+AD +TYN H+I RF+L
Sbjct: 308 RSRAFWAAYFGDWRDTFPEQLAGVTEEEMYRAVNTVSRSLIRTDADELTYNLHVITRFEL 367
Query: 372 ERLMVSEEVKAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSI-GYFPTYSIGTI 430
ER M++ ++ DL + W+ E+ LG+R S +G LQD+HW G I G F Y+IG +
Sbjct: 368 EREMLAGKLAVRDLADAWHAAYEQNLGLRAPSDVDGALQDVHWYFGPIGGSFQGYTIGNV 427
Query: 431 LSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAIGEEMDAE 490
LSAQ Y + P E A+ +F + WLRE ++R G + P EL+ RA G+ + A
Sbjct: 428 LSAQFYAAAEAANPGLEADFARKDFSRLHGWLRENVYRHGRRWTPGELIERATGQALTAG 487
Query: 491 YFIRWIEERY 500
+++++ +Y
Sbjct: 488 PYLKYLRGKY 497
>gb|AAA96610.1| (L77246) putative [Bacillus subtilis]
Length = 364
Score = 296 bits (750), Expect = 4e-79
Identities = 146/360 (40%), Positives = 221/360 (60%), Gaps = 3/360 (0%)
Query: 142 ELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKKLRPLLDKILEEGKVPRE 201
E K+ Y GY+E PYDALLDL+E G+ + + ++F L++ + PL+ ++ G P
Sbjct: 1 EFNKRFITYWGYQEHPYDALLDLFEPGVTVKVLDQLFAELKEAIIPLVKQVTASGNKPDT 60
Query: 202 HELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFTTEFGIRDVRITTRYEGFD 261
+ K + +E + ++L+ L++ GY RLD + HPF T DVR+TTRY+ D
Sbjct: 61 SFITKA-FPKEKQKELSLYFLQELGYDFDG-GRLDETVHPFATTLNRGDVRVTTRYDEKD 118
Query: 262 FRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGIHESQSRFWENIIGRSREFVELI 321
FR + T+HE GHA+YE DE T ++ G S+GIHESQS F+EN IGR++ F
Sbjct: 119 FRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLFYENFIGRNKHFWTPY 178
Query: 322 YPVLKENLPFIDK-YTQEDVYLYFNMVRPDFIRTEADVVTYNFHIILRFKLERLMVSEEV 380
Y ++E P K + +D N +P FIR EAD +TY HII+R+++E+ + S EV
Sbjct: 179 YKKIQEASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHIIIRYEIEKAIFSNEV 238
Query: 381 KAGDLPEMWNEEMERLLGIRPKSYKEGILQDIHWAHGSIGYFPTYSIGTILSAQLYYHIK 440
DLP +WN++ + LGI P++ EGILQD+HWA G GYFP+Y++G + +AQL +
Sbjct: 239 SVEDLPSLWNQKYQDYLGITPQTDAEGILQDVHWAGGDFGYFPSYALGYMYAAQLKQKML 298
Query: 441 RDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYPPKELLRRAIGEEMDAEYFIRWIEERY 500
D+P+F+ + +GEF PIK WL EK+H G P ++++ A GEE++ Y I ++ +Y
Sbjct: 299 EDLPEFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGEELNVRYLIDYLSNKY 358
>pir||B82202 thermostable carboxypeptidase 1 VC1414 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9655911|gb|AAF94571.1|
(AE004220) thermostable carboxypeptidase 1 [Vibrio
cholerae]
Length = 524
Score = 275 bits (695), Expect = 1e-72
Identities = 162/507 (31%), Positives = 276/507 (53%), Gaps = 12/507 (2%)
Query: 1 MEGQEVFKNETIKKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQ 60
+ ++ K+++ ++I H S++GWD MP G RS A ELSV
Sbjct: 23 LSSRKAISMNAFNKLVKHSKKISNFNHLSSIVGWDQAAVMPSGGAEARSQAMAELSVHIH 82
Query: 61 ELLLKPYFVELVEKAKGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKA 120
L+ +P + +A+ E LN ++ +R + RQ + P + S SK A
Sbjct: 83 GLMTQPQLADWFAEAES-ETLNIEQQATLREMKRQWQQATVLPEALVEAQSLAGSKCEHA 141
Query: 121 WEEAKAKDDFSKFEPWLDKIIELAKKAAEYLGYE--EEPYDALLDLYEEGLRTRDVVKMF 178
W + +D++ FE ++++L+++ A+ + PYDA+L+LYE G T + ++F
Sbjct: 142 WRSQRKNNDWTGFEKNWAEVVKLSQEEAQIRAEATGKTPYDAMLELYEPGTTTEQLNRVF 201
Query: 179 EVLEKKLRPLLDKILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVS 238
++ L L+D ++E+ + K Y E + + L V++ + RLD S
Sbjct: 202 SDVKTWLPSLIDVVIEK-QSSESFIAPKGHYPAESQKALGLDVMKLLQFDFN-HGRLDES 259
Query: 239 AHPFTTEFGI-RDVRITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSL 297
HPF G+ DVRITTRY +F ++L+ VHE GHA YE + TP S+
Sbjct: 260 VHPFCG--GVPSDVRITTRYNESEFVQSLMGIVHETGHARYEQGLPKHLAGTPAGEARSM 317
Query: 298 GIHESQSRFWENIIGRSREFVELIYPVLKENLPFIDK--YTQEDVYLYFNMVRPDFIRTE 355
GIHESQS F+E +GRS F+ + + ++ ++ + E++ + V+ DFIR +
Sbjct: 318 GIHESQSLFFEMQVGRSPAFIAHLAELAGKHFSAMNDPVFRVENIQKLYTRVQKDFIRVD 377
Query: 356 ADVVTYNFHIILRFKLERLMVSEEVKAGDLPEMWNEEMERLLGIRPK-SYKEGILQDIHW 414
AD +TY H+ILRF++ER +++ ++K D+PE+W+++M+ LG+ K + + G +QDIHW
Sbjct: 378 ADELTYPAHVILRFEIERDLMNGKIKHTDVPELWDQKMQAYLGLSTKGNDQNGCMQDIHW 437
Query: 415 AHGSIGYFPTYSIGTILSAQLYYHIKRDIPDFEEKVAKGEFEPIKVWLREKIHRWGSIYP 474
GS GYFP+Y++G + +AQ +K+ + D + + +G+ PI WL E I GS++
Sbjct: 438 TDGSFGYFPSYTLGAMYAAQFMAAMKKTV-DVDGAIRRGDLSPIFTWLSENIWSKGSLFS 496
Query: 475 PKELLRRAIGEEMDAEYFIRWIEERYL 501
EL+++A GE ++ E+F + +RYL
Sbjct: 497 TDELVKQATGETLNPEHFKAHLSQRYL 523
>gb|AAB38482.1| (L47838) putative [Bacillus subtilis]
Length = 293
Score = 177 bits (445), Expect = 2e-43
Identities = 99/288 (34%), Positives = 156/288 (53%), Gaps = 3/288 (1%)
Query: 13 KKILEKYRRIWALGHAQSVLGWDLEVNMPKEGILERSVAQGELSVLSQELLLKPYFVELV 72
K+ + +RI A +++ WD PK G +R+ + G+LS + EL+
Sbjct: 8 KEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFNIQTSDRMKELI 67
Query: 73 EKA-KGIEDLNEYERGVVRVLDRQIRIMKSFPPEFLREVSETTSKATKAWEEAKAKDDFS 131
+ + +DL+E + V + ++ K P +E SKA AWEEAK K DFS
Sbjct: 68 DVLYERFDDLSEDTKKAVELAKKEYEENKKIPEAEYKEYVILCSKAETAWEEAKGKSDFS 127
Query: 132 KFEPWLDKIIELAKKAAEYLGYEEEPYDALLDLYEEGLRTRDVVKMFEVLEKKLRPLLDK 191
F P+L+++IE K+ Y GY+E PYDALLDL+E G+ + + ++F L++ + PL+ +
Sbjct: 128 LFSPYLEQLIEFNKRFITYWGYQEHPYDALLDLFEPGVTVKVLDQLFAELKEAIIPLVKQ 187
Query: 192 ILEEGKVPREHELEKEKYEREWMERVNLWVLEKFGYPLGTRARLDVSAHPFTTEFGIRDV 251
+ G P + K + +E + ++L+ L++ GY RLD + HPF T DV
Sbjct: 188 VTASGNKPDTSFITK-AFPKEKQKELSLYFLQELGYDF-DGGRLDETVHPFATTLNRGDV 245
Query: 252 RITTRYEGFDFRRTLLSTVHEFGHALYELQQDERFMFTPIAGGVSLGI 299
R+TTRY+ DFR + T+HE GHA+YE DE T ++ G S+GI
Sbjct: 246 RVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGI 293
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.140 0.417
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 192708853
Number of Sequences: 2977
Number of extensions: 8416840
Number of successful extensions: 23108
Number of sequences better than 1.0e-10: 12
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 23062
Number of HSP's gapped (non-prelim): 13
length of query: 501
length of database: 189,106,746
effective HSP length: 55
effective length of query: 446
effective length of database: 156,189,961
effective search space: 69660722606
effective search space used: 69660722606
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 167 (69.5 bits)