BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1331 (cheB) DE:protein-glutamate methylesterase (cheB) (368 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75002 protein-glutamate methylesterase (cheb) PAB1331 - Py... 721 0.0 sp|O58192|CHEB_PYRHO PROTEIN-GLUTAMATE METHYLESTERASE >gi|743507... 701 0.0 sp|Q9WYN9|CHEB_THEMA PROTEIN-GLUTAMATE METHYLESTERASE >gi|743506... 353 2e-96 pir||F83623 probable methylesterase PA0173 [imported] - Pseudomo... 302 4e-81 gi|11498646 protein-glutamate methylesterase (cheB) [Archaeoglob... 291 9e-78 dbj|BAB06154.1| (AP001515) MCP-glutamate methylesterase / two-co... 288 6e-77 sp|P07330|CHEB_ECOLI PROTEIN-GLUTAMATE METHYLESTERASE >gi|67229|... 279 4e-74 gb|AAA23569.1| (M13463) cheB peptide [Escherichia coli] 277 1e-73 sp|P04042|CHEB_SALTY PROTEIN-GLUTAMATE METHYLESTERASE >gi|67228|... 277 2e-73 dbj|BAB15840.1| (AB040876) CheB [Enterobacter cloacae] 272 5e-72 sp|Q05522|CHEB_BACSU PROTEIN-GLUTAMATE METHYLESTERASE >gi|107589... 267 1e-70 sp|O87717|CHEB_CAUCR PROTEIN-GLUTAMATE METHYLESTERASE >gi|338736... 265 8e-70 sp|Q52883|CHEB_RHIME PROTEIN-GLUTAMATE METHYLESTERASE >gi|212079... 262 4e-69 sp|O85128|CHEB_AGRTU PROTEIN-GLUTAMATE METHYLESTERASE >gi|328279... 257 2e-67 gb|AAG19394.1| (AE005033) chemotaxis protein; CheB [Halobacteriu... 255 7e-67 sp|Q48300|CHEB_HALSA PROTEIN-GLUTAMATE METHYLESTERASE >gi|136346... 255 7e-67 pir||H82122 protein-glutamate methylesterase CheB VC2062 [import... 244 1e-63 gb|AAF32418.1| (AF069392) chemotaxis protein CheB [Vibrio paraha... 243 3e-63 sp|O52262|CHEB_PSEPU PROTEIN-GLUTAMATE METHYLESTERASE >gi|285360... 242 7e-63 sp|O87125|CHEB_PSEAE PROTEIN-GLUTAMATE METHYLESTERASE >gi|112626... 241 1e-62 pir||G83463 probable methyltransferase PA1459 [imported] - Pseud... 239 3e-62 gb|AAF67048.1|AF183382_8 (AF183382) methylesterase CheB [Pseudom... 238 8e-62 sp|O83639|CHEB_TREPA PROTEIN-GLUTAMATE METHYLESTERASE >gi|743506... 235 5e-61 sp|P72253|CHEB_RHOCE PROTEIN-GLUTAMATE METHYLESTERASE >gi|162128... 216 3e-55 sp|O33558|CHEB_RHOSH PROTEIN-GLUTAMATE METHYLESTERASE >gi|112626... 215 8e-55 pir||A82206 protein-glutamate methylesterase CheB VC1401 [import... 200 2e-50 sp|O51376|Y415_BORBU PROBABLE PROTEIN-GLUTAMATE METHYLESTERASE B... 178 7e-44 sp|P31758|FRZG_MYXXA PROTEIN-GLUTAMATE METHYLESTERASE FRZG >gi|9... 169 3e-41 pir||F83183 probable methylesterase PA3703 [imported] - Pseudomo... 166 5e-40 sp|Q45047|CHEB_BORBU PROTEIN-GLUTAMATE METHYLESTERASE >gi|743506... 164 2e-39 pdb|1CHD| Cheb Methylesterase Domain 159 5e-38 sp|Q56312|CHEY_THEMA CHEMOTAXIS PROTEIN CHEY >gi|7443033|pir||B7... 96 5e-19 emb|CAA53764.1| (X76170) cheY [Listeria monocytogenes] 95 1e-18 gi|11498647 chemotaxis response regulator (cheY) [Archaeoglobus ... 87 2e-16 gb|AAC27631.1| (AF076485) defective in fruiting DifD [Myxococcus... 87 4e-16 pir||E71160 probable chemotaxis protein (cheY) - Pyrococcus hori... 85 9e-16 pir||F75002 chemotaxis response regulator (chey) PAB1330 - Pyroc... 85 1e-15 dbj|BAB06687.1| (AP001517) two-component response regulator invo... 84 3e-15 dbj|BAB06163.1| (AP001515) two-component response regulator invo... 82 1e-14 sp|P45709|CCDB_BACSU CCDB PROTEIN >gi|1361404|pir||S57405 respon... 81 2e-14 emb|CAB99140.1| (AL390188) putative two-component system respons... 81 2e-14 sp|P24072|CHEY_BACSU CHEMOTAXIS PROTEIN CHEY HOMOLOG >gi|98251|p... 80 4e-14 sp|P54662|DEGU_BACBR TRANSCRIPTIONAL REGULATORY PROTEIN DEGU >gi... 78 2e-13 pir||T35758 probable response regulator - Streptomyces coelicolo... 78 2e-13 sp|P52931|SP0A_BACCI STAGE 0 SPORULATION PROTEIN A >gi|497953|gb... 77 3e-13 pir||S60871 phosphorylation-activated transcription factor Spo0A... 77 3e-13 sp|P52934|SP0A_BACST STAGE 0 SPORULATION PROTEIN A >gi|2654216|e... 77 3e-13 gb|AAD16452.1| (AF102271) response-regulator [Streptomyces coeli... 76 5e-13 dbj|BAB07347.1| (AP001519) two-component response regulator invo... 76 8e-13 sp|P55184|YXJL_BACSU HYPOTHETICAL TRANSCRIPTIONAL REGULATOR IN G... 75 1e-12 emb|CAA63989.1| (X94328) spo0a [Clostridium difficile] 75 1e-12 sp|P52938|SP0A_CLODI STAGE 0 SPORULATION PROTEIN A HOMOLOG >gi|1... 75 1e-12 pir||H70558 hypothetical protein Rv1626 - Mycobacterium tubercul... 75 1e-12 pir||T34653 probable response regulator - Streptomyces coelicolo... 75 1e-12 emb|CAB59507.1| (AL132648) putative two-component response regul... 75 1e-12 pir||T36318 two-component response regulator - Streptomyces coel... 75 2e-12 pir||T36530 probable two-component system regulator - Streptomyc... 75 2e-12 pir||A70080 two-component response regulator [YxjM] homolog yxjL... 74 2e-12 pir||G69780 two-component response regulator [YdfH] homolog ydfI... 74 2e-12 pir||T35281 probable two component response regulator - Streptom... 74 2e-12 pir||H75381 DNA-binding response regulator - Deinococcus radiodu... 74 2e-12 emb|CAB54571.1| (AJ006393) response regulator [Streptococcus pne... 73 4e-12 emb|CAB87987.1| (AJ288948) Spo0A protein [Clostridium cellulolyt... 73 4e-12 pir||T36528 probable two-component system regulator - Streptomyc... 73 4e-12 pir||T30314 chpB protein - Pseudomonas aeruginosa >gi|11351447|p... 73 5e-12 pir||T35166 probable two-component regulator - Streptomyces coel... 73 5e-12 pir||T36862 probable two-component response regulator - Streptom... 73 5e-12 sp|P33356|YEHT_ECOLI HYPOTHETICAL 27.9 KD PROTEIN IN MOLR-BGLX I... 73 7e-12 gb|AAC33471.1| (AF074950) CheY [Treponema denticola] 73 7e-12 pdb|1DZ3|A Chain A, Domain-Swapping In The Sporulation Response ... 73 7e-12 emb|CAB90919.1| (AL355774) putative DNA-binding response regulat... 73 7e-12 emb|CAB95289.1| (AL359779) putative two component system respons... 73 7e-12 sp|P37478|YYCF_BACSU HYPOTHETICAL 27.2 KD SENSORY TRANSDUCTION P... 72 9e-12 pir||S58645 response regulator cheY [validated] - Halobacterium ... 72 9e-12 pir||I40540 vsrD protein - Pseudomonas solanacearum >gi|603069|g... 72 1e-11 dbj|BAA88407.1| (AB016841) chiR [Streptomyces thermoviolaceus] 72 1e-11 dbj|BAB05816.1| (AP001514) two-component response regulator [Bac... 72 1e-11 gb|AAB07804.1| (U01971) MtrA [Mycobacterium tuberculosis] 71 2e-11 pir||H70592 probable mtrA protein - Mycobacterium tuberculosis (... 71 2e-11 pir||T34675 probable response regulator - Streptomyces coelicolo... 71 2e-11 sp|P52932|SP0A_BACME STAGE 0 SPORULATION PROTEIN A >gi|497955|gb... 71 3e-11 pir||F70645 hypothetical protein Rv3133c - Mycobacterium tubercu... 71 3e-11 pir||S60872 phosphorylation-activated transcription factor Spo0A... 71 3e-11 pir||F75593 DNA-binding response regulator - Deinococcus radiodu... 71 3e-11 pir||C81104 transcription regulator, LuxR family NMB1250 [import... 71 3e-11 pir||H82218 transcription regulator LuxR family VC1277 [imported... 71 3e-11 dbj|BAB05842.1| (AP001514) two-component response regulator [Bac... 71 3e-11 dbj|BAB05932.1| (AP001514) two-component response regulator [Bac... 71 3e-11 dbj|BAB07746.1| (AP001520) two-component response regulator [Bac... 71 3e-11 sp|P52940|SP0A_CLOPA STAGE 0 SPORULATION PROTEIN A HOMOLOG >gi|4... 70 3e-11 pir||S60880 phosphorylation-activated transcription factor Spo0A... 70 3e-11 pir||E75450 DNA-binding response regulator - Deinococcus radiodu... 70 3e-11 sp|P13800|DEGU_BACSU TRANSCRIPTIONAL REGULATORY PROTEIN DEGU (PR... 70 4e-11 pdb|1QMP|A Chain A, Phosphorylated Aspartate In The Crystal Stru... 70 4e-11 emb|CAB64972.1| (AJ012050) VicR protein [Enterococcus faecalis] 70 4e-11 sp|P52929|SP0A_BACBR STAGE 0 SPORULATION PROTEIN A >gi|2119860|p... 69 6e-11 pir||T35199 probable two-component regulator - Streptomyces coel... 69 6e-11 dbj|BAB07561.1| (AP001520) two-component response regulator [Bac... 69 6e-11 sp|P96126|CHEY_TREPA CHEMOTAXIS PROTEIN CHEY >gi|7443016|pir||D7... 69 8e-11 gi|11467627 ompR homolog [Guillardia theta] >gi|6136611|sp|O7842... 69 8e-11 pir||T36543 probable two-component system regulator - Streptomyc... 69 8e-11 >pir||E75002 protein-glutamate methylesterase (cheb) PAB1331 - Pyrococcus abyssi (strain Orsay) >gi|5458973|emb|CAB50459.1| (AJ248288) protein-glutamate methylesterase (cheB) [Pyrococcus abyssi] Length = 368 Score = 721 bits (1841), Expect = 0.0 Identities = 368/368 (100%), Positives = 368/368 (100%) Query: 1 MPLTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLD 60 MPLTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLD Sbjct: 1 MPLTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLD 60 Query: 61 VEMPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINM 120 VEMPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINM Sbjct: 61 VEMPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINM 120 Query: 121 RELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSL 180 RELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSL Sbjct: 121 RELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSL 180 Query: 181 LKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG 240 LKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG Sbjct: 181 LKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG 240 Query: 241 DYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQG 300 DYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQG Sbjct: 241 DYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQG 300 Query: 301 IVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIRGGGNL 360 IVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIRGGGNL Sbjct: 301 IVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIRGGGNL 360 Query: 361 GRHVTIPR 368 GRHVTIPR Sbjct: 361 GRHVTIPR 368 >sp|O58192|CHEB_PYRHO PROTEIN-GLUTAMATE METHYLESTERASE >gi|7435070|pir||F71160 hypothetical protein PH0483 - Pyrococcus horikoshii >gi|3256888|dbj|BAA29571.1| (AP000002) 368aa long hypothetical protein-glutamate methylesterase [Pyrococcus horikoshii] Length = 368 Score = 701 bits (1790), Expect = 0.0 Identities = 356/368 (96%), Positives = 365/368 (98%) Query: 1 MPLTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLD 60 MPL GRKI+VLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLD Sbjct: 1 MPLMGRKIKVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLD 60 Query: 61 VEMPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINM 120 VEMPRMNGLDALRVIMK+YPVPVIMISALTQEGA+ATIKALEYGAIDFIPKPSSSISINM Sbjct: 61 VEMPRMNGLDALRVIMKRYPVPVIMISALTQEGADATIKALEYGAIDFIPKPSSSISINM 120 Query: 121 RELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSL 180 +ELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSL Sbjct: 121 KELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSL 180 Query: 181 LKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG 240 LKIFPKFPE+LKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG Sbjct: 181 LKIFPKFPEDLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG 240 Query: 241 DYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQG 300 DYHMEVTLR GKPVITLNKKPK+HGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQG Sbjct: 241 DYHMEVTLRAGKPVITLNKKPKIHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQG 300 Query: 301 IVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIRGGGNL 360 IVEIKK+GGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVV+AVE+IRGGGNL Sbjct: 301 IVEIKKKGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVKAVEIIRGGGNL 360 Query: 361 GRHVTIPR 368 GRHVTI R Sbjct: 361 GRHVTISR 368 >sp|Q9WYN9|CHEB_THEMA PROTEIN-GLUTAMATE METHYLESTERASE >gi|7435065|pir||F72380 protein-glutamate methylesterase - Thermotoga maritima (strain MSB8) >gi|4980913|gb|AAD35493.1|AE001720_7 (AE001720) protein-glutamate methylesterase [Thermotoga maritima] Length = 344 Score = 353 bits (896), Expect = 2e-96 Identities = 174/349 (49%), Positives = 249/349 (70%), Gaps = 5/349 (1%) Query: 3 LTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVE 62 +T R IRVLVVDDSAFMR VLKDII+S P+++V G A+DG+EA+E + +PDV+T+D+E Sbjct: 1 MTDRVIRVLVVDDSAFMRMVLKDIIDSQPDMKVVGFAKDGLEAVEKAIELKPDVITMDIE 60 Query: 63 MPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRE 122 MP +NG++AL++IMKK P VIM+S+LT+EGA TI+AL GA+DFI KP SIS+ R+ Sbjct: 61 MPNLNGIEALKLIMKKAPTRVIMVSSLTEEGAAITIEALRNGAVDFITKPHGSISLTFRQ 120 Query: 123 LKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLK 182 + E++ KI++A V R L + +V + KP+V +I V I +STGGP+SL Sbjct: 121 VAPELLEKIRQAMNVDPRTLLFKP----KVSRLTITKPAVSGKIVV-IGSSTGGPRSLDM 175 Query: 183 IFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDY 242 I P P+N A I++VQHMPPGFT+S A RLDS S++ VKEAE+G+ V+ G Y+APGD+ Sbjct: 176 IIPNLPKNFPAPIVVVQHMPPGFTKSLAMRLDSTSELTVKEAEDGEEVKPGFVYIAPGDF 235 Query: 243 HMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIV 302 H+ + + GK L+K K++ VRPA D + AA+++ +T+ V++TGMG+DG +G Sbjct: 236 HLGLKAQNGKVFFFLDKSDKINNVRPAVDFTLDKAAEIYKSKTIAVILTGMGKDGTKGAF 295 Query: 303 EIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAV 351 ++K GG IA+D++T ++FGMPK+ IE G DYV+P KIPE ++ V Sbjct: 296 KVKFYGGTVIAEDKETCVVFGMPKSVIEEGYADYVLPAYKIPEKLIELV 344 >pir||F83623 probable methylesterase PA0173 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9946006|gb|AAG03563.1|AE004455_4 (AE004455) probable methylesterase [Pseudomonas aeruginosa] Length = 349 Score = 302 bits (765), Expect = 4e-81 Identities = 157/352 (44%), Positives = 232/352 (65%), Gaps = 7/352 (1%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I VLVVDDSA +R +LK+II +DPEL + G A D A +++++ PDV++LDVEMPRM+ Sbjct: 3 ISVLVVDDSALIRSLLKEIIQADPELRLVGCAPDAFVARDLIKQHAPDVISLDVEMPRMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 GL L +MK P PV+MIS+LT+ G+EAT++ALE GA+DFI KP I+ M+ +EI Sbjct: 63 GLTFLDKLMKARPTPVLMISSLTERGSEATLRALELGAVDFIAKPRLGIAEGMQAYAEEI 122 Query: 128 IAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKF 187 AK+K A+ LRR + P + +A+ ASTGG ++L ++ Sbjct: 123 RAKLKTVARA-----RLRRRAADAPAPPESAAPLLSTEKIIALGASTGGTEALKEVLLGL 177 Query: 188 PENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVT 247 P + G+++ QHMPPGFTRSFA+RLD ++++ V EA +GD + G A VAPGD+HMEV Sbjct: 178 PAH-SPGVVITQHMPPGFTRSFAERLDRLTRLSVSEARDGDRILPGHALVAPGDHHMEVQ 236 Query: 248 LRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKR 307 G V+ LN++ +++G RPA D M + A+ GR + ++TGMG+DGA+G++ I++ Sbjct: 237 RSGANYVVRLNRQAQVNGHRPAVDVMFESLARCAGRNLLAGLLTGMGKDGARGLLAIRQA 296 Query: 308 GGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIRGGGN 359 GG T+AQDE T +++GMP+ A+E G + V+PLE+I +++ RG GN Sbjct: 297 GGYTLAQDEATCVVYGMPREAVELGAAEDVLPLERIAAALLQQAAR-RGSGN 347 >gi|11498646 protein-glutamate methylesterase (cheB) [Archaeoglobus fulgidus] >gi|7531056|sp|O29221|CHEB_ARCFU PROTEIN-GLUTAMATE METHYLESTERASE >gi|7435066|pir||A69380 protein-glutamate methylesterase (EC 3.1.1.61) cheB - Archaeoglobus fulgidus >gi|2649549|gb|AAB90199.1| (AE001031) protein-glutamate methylesterase (cheB) [Archaeoglobus fulgidus] Length = 349 Score = 291 bits (737), Expect = 9e-78 Identities = 146/346 (42%), Positives = 225/346 (64%), Gaps = 2/346 (0%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +RVLVVDDSA +R + DI+ +EV A++G EA+E K +PDV+TLD+ MP M+ Sbjct: 1 MRVLVVDDSALVRMAVTDILTK-AGIEVVDTAKNGREAVEKALKLKPDVITLDINMPEMD 59 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 GL AL++IM+K P PV+M+S+LT+EGA T++AL+ GA+DF+ KP ++ +++ + E+ Sbjct: 60 GLTALKLIMEKQPTPVVMLSSLTKEGARETLEALKIGAVDFVTKPDGAL-VDLSSVAQEL 118 Query: 128 IAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKF 187 + K++ AA L L+ ++ ++ + + + V I +STGGP +L I P+ Sbjct: 119 VRKVQMAASTSLNVLRLQNLKKIKGEVVRGNWKGKTKDVCVLIGSSTGGPSALEMIIPRL 178 Query: 188 PENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVT 247 P ++ A + +VQHMPPGFT+ A+RL+S+S+++VKEAE + V++G YVAPG YHM+V Sbjct: 179 PADIPAPVFVVQHMPPGFTKQLAERLNSISEVEVKEAENNERVKDGVVYVAPGGYHMKVR 238 Query: 248 LRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKR 307 I + ++ V+P+ D + Q +G VG ++TGMG DGA G+ IK R Sbjct: 239 RAANVVRIKVVDGEPVNAVKPSVDVTADSVVQAYGGNVVGAILTGMGEDGAYGMKLIKDR 298 Query: 308 GGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEM 353 GG+TIA E T ++FGMPKAAIE G + V P+ +I E +VR +E+ Sbjct: 299 GGLTIASSEDTCMVFGMPKAAIELGGITSVKPVFEIAEEIVRFLEV 344 >dbj|BAB06154.1| (AP001515) MCP-glutamate methylesterase / two-component response regulator [Bacillus halodurans] Length = 346 Score = 288 bits (730), Expect = 6e-77 Identities = 146/343 (42%), Positives = 225/343 (65%), Gaps = 4/343 (1%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 +I+VLVVDDSAFMRKV+ ++ SDP LEV G AR+G +A++ + PDV+TLDVEMP M Sbjct: 4 RIKVLVVDDSAFMRKVITSMLESDPRLEVVGTARNGEDALKKRKTLAPDVMTLDVEMPVM 63 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +G++ L+ +M + P+P+I++S+ T+EGAE + A+E GA+DFI KPS IS+++ +++D Sbjct: 64 DGIETLKRLMSENPLPIIIVSSTTKEGAENALTAMELGAVDFITKPSGPISLDLEKVRDL 123 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPK 186 +I K+ A V + + + R + +P + + I STGGP++L ++ K Sbjct: 124 LIEKVASAPNVKLKPTSKKAPVVFRPLQ----QPVPFDKTIIVIGTSTGGPKALKQVLTK 179 Query: 187 FPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEV 246 P++L A IL+VQHMP GFT S A RL+S+ +I VKEAE+G+++ G AY+APG YH+E Sbjct: 180 LPKDLPAPILIVQHMPAGFTESLANRLNSLCEIHVKEAEDGEIIRPGVAYIAPGGYHLET 239 Query: 247 TLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKK 306 G + L K+ G RP+ D + + + + V V+MTGMG DG +G+ ++K+ Sbjct: 240 KRLGTSIALHLTKEEPRKGHRPSVDVLFESVSALSSIYKVAVIMTGMGSDGTEGLEQLKR 299 Query: 307 RGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVR 349 IA+ E++ ++FGMPK AIE +VD V LE I +++VR Sbjct: 300 GSCYAIAESEQSCVVFGMPKVAIERKLVDEVTHLELIADSIVR 342 >sp|P07330|CHEB_ECOLI PROTEIN-GLUTAMATE METHYLESTERASE >gi|67229|pir||XYECEB protein-glutamate methylesterase (EC 3.1.1.61) - Escherichia coli >gi|1736536|dbj|BAA15699.1| (D90830) Protein-glutamate methylesterase (EC 3.1.1.61). [Escherichia coli] >gi|1788192|gb|AAC74953.1| (AE000282) response regulator for chemotaxis (cheA sensor); protein methylesterase [Escherichia coli K12] Length = 349 Score = 279 bits (706), Expect = 4e-74 Identities = 147/345 (42%), Positives = 221/345 (63%), Gaps = 6/345 (1%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIRVL VDDSA MR+++ +IINS ++E+ A D + A ++++K PDV+TLDVEMPRM Sbjct: 3 KIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRM 62 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +GLD L +M+ P+PV+M+S+LT +G+E T++ALE GAIDF+ KP I M + Sbjct: 63 DGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYNEM 122 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPK 186 I K++ AAK L + L K P + + +AI ASTGG +++ + Sbjct: 123 IAEKVRTAAKA-----SLAAHKPLSAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQP 177 Query: 187 FPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEV 246 P + A +L+ QHMPPGFTRSFA RL+ + +I VKEAE+G+ V G AY+APGD HME+ Sbjct: 178 LPLSSPA-LLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMEL 236 Query: 247 TLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKK 306 + G I ++ P ++ RP+ D + + A+ GR VGV++TGMG DGA G++ +++ Sbjct: 237 SRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAVGVILTGMGNDGAAGMLAMRQ 296 Query: 307 RGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAV 351 G T+AQ+E + ++FGMP+ AI G V VV L ++ + ++ + Sbjct: 297 AGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQMLAKI 341 >gb|AAA23569.1| (M13463) cheB peptide [Escherichia coli] Length = 349 Score = 277 bits (701), Expect = 1e-73 Identities = 146/345 (42%), Positives = 220/345 (63%), Gaps = 6/345 (1%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIRVL VDDSA MR+++ +IINS ++E+ A D + A ++++K PDV+TLDVEMPRM Sbjct: 3 KIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRM 62 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +GLD L +M+ P+PV+M+S+LT +G+E T++ LE GAIDF+ KP I M + Sbjct: 63 DGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRPLELGAIDFVTKPQLGIREGMLAYNEM 122 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPK 186 I K++ AAK L + L K P + + +AI ASTGG +++ + Sbjct: 123 IAQKVRTAAKA-----SLAAHKPLSAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQP 177 Query: 187 FPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEV 246 P + A +L+ QHMPPGFTRSFA RL+ + +I VKEAE+G+ V G AY+APGD HME+ Sbjct: 178 LPLSSPA-LLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMEL 236 Query: 247 TLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKK 306 + G I ++ P ++ RP+ D + + A+ GR VGV++TGMG DGA G++ +++ Sbjct: 237 SRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAVGVILTGMGNDGAAGMLAMRQ 296 Query: 307 RGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAV 351 G T+AQ+E + ++FGMP+ AI G V VV L ++ + ++ + Sbjct: 297 AGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQMLAKI 341 >sp|P04042|CHEB_SALTY PROTEIN-GLUTAMATE METHYLESTERASE >gi|67228|pir||XYEBET protein-glutamate methylesterase (EC 3.1.1.61) - Salmonella typhimurium >gi|3212266|pdb|1A2O|A Chain A, Structural Basis For Methylesterase Cheb Regulation By A Phosphorylation-Activated Domain >gi|3212267|pdb|1A2O|B Chain B, Structural Basis For Methylesterase Cheb Regulation By A Phosphorylation-Activated Domain Length = 349 Score = 277 bits (700), Expect = 2e-73 Identities = 144/345 (41%), Positives = 220/345 (63%), Gaps = 6/345 (1%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIRVL VDDSA MR+++ +IINS ++E+ A D + A ++++K PDV+TLDVEMPRM Sbjct: 3 KIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRM 62 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +GLD L +M+ P+PV+M+S+LT +G+E T++ALE GAIDF+ KP I M + Sbjct: 63 DGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYSEM 122 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPK 186 I K++ AA+ + + + K P + + +AI ASTGG +++ + Sbjct: 123 IAEKVRTAARA-----RIAAHKPMAAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQP 177 Query: 187 FPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEV 246 P + A +++ QHMPPGFTRSFA+RL+ + +I VKEAE+G+ V G AY+APGD HME+ Sbjct: 178 LPLSSPA-VIITQHMPPGFTRSFAERLNKLCQISVKEAEDGERVLPGHAYIAPGDKHMEL 236 Query: 247 TLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKK 306 G I ++ P ++ RP+ D + + A+ GR VGV++TGMG DGA G++ + + Sbjct: 237 ARSGANYQIKIHDGPPVNRHRPSVDVLFHSVAKHAGRNAVGVILTGMGNDGAAGMLAMYQ 296 Query: 307 RGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAV 351 G TIAQ+E + ++FGMP+ AI G V VV L ++ + ++ + Sbjct: 297 AGAWTIAQNEASCVVFGMPREAINMGGVSEVVDLSQVSQQMLAKI 341 >dbj|BAB15840.1| (AB040876) CheB [Enterobacter cloacae] Length = 350 Score = 272 bits (688), Expect = 5e-72 Identities = 144/337 (42%), Positives = 214/337 (62%), Gaps = 6/337 (1%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIRVL VDDSA MR+++ +IINS ++E+ A D + A ++++K PDV+TLDVEMPRM Sbjct: 3 KIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKYNPDVLTLDVEMPRM 62 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +G+D L +M+ P+PV+M+S+LT +G+E T++ALE GA+DF+ KP I M + Sbjct: 63 DGIDFLEKLMRLRPMPVVMVSSLTGKGSEITLRALELGAVDFVTKPQLGIREGMLAYSEM 122 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPK 186 I KI+ ++ +L L K P + + + I ASTGG +++ + Sbjct: 123 IAEKIRHPSRA-----KLAAHTPLAAPDTLKAGPLLSSEKLLVIGASTGGTEAIRHVLQP 177 Query: 187 FPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEV 246 P + GIL+ QHMPPGFTRSFA+RL+ + +I VKEAE+G+ V G AY+APGD HME+ Sbjct: 178 LPLS-SPGILITQHMPPGFTRSFAERLNKLCQISVKEAEDGERVPPGHAYIAPGDKHMEL 236 Query: 247 TLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKK 306 + R I ++ P ++ RP+ D + + A+ GR VGV++TGMG DGA G++ + Sbjct: 237 SRRRANYQIKIHDGPPVNRHRPSVDVLFHSVAKHAGRNAVGVILTGMGNDGAAGMLAMPI 296 Query: 307 RGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKI 343 G TIAQ+E + ++FGMP+ AI G V VV L ++ Sbjct: 297 AGAWTIAQNEASCVVFGMPREAINMGGVSEVVDLSQV 333 >sp|Q05522|CHEB_BACSU PROTEIN-GLUTAMATE METHYLESTERASE >gi|1075896|pir||A48511 protein-glutamate methylesterase (EC 3.1.1.61) cheB - Bacillus subtilis >gi|580836|emb|CAA48007.1| (X67806) CheB [Bacillus subtilis] >gi|2634014|emb|CAB13515.1| (Z99112) methyl-accepting chemotaxis proteins (MCP)-glutamate methylesterase and two-component response regulator-like [Bacillus subtilis] Length = 357 Score = 267 bits (676), Expect = 1e-70 Identities = 142/358 (39%), Positives = 226/358 (62%), Gaps = 13/358 (3%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVLVVDDSAFMRK++ D + + ++EV G AR+G EA++ ++ +PDV+TLDVEMP MN Sbjct: 2 IRVLVVDDSAFMRKMISDFLTEEKQIEVIGTARNGEEALKKIELLKPDVITLDVEMPVMN 61 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G D +R I++ Y +PVIM+S+ T++G E TI LE GA DFI KPS SIS+++ ++K+++ Sbjct: 62 GTDTVRKIIEIYNLPVIMVSSQTEKGKECTINCLEIGAFDFITKPSGSISLDLYKIKEQL 121 Query: 128 IAKIKEAAKVPRRFLELRR-------IRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSL 180 + ++ A +R + + +R + + K KP +I V I STGGP++L Sbjct: 122 VERVVAAGLSGKRKRPVSQTVRPEPIVRAVVKPELSKPKPGTGRQI-VCIGTSTGGPRAL 180 Query: 181 LKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG 240 K+ PK P++L A +++VQHMP GFT S A RL+ +S I VKEA++G+ G Y+APG Sbjct: 181 QKVIPKLPKDLNAPVVVVQHMPEGFTASLADRLNHLSDIQVKEAKDGEAALNGCVYIAPG 240 Query: 241 DYHMEV--TLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGA 298 ++ V G + V+ + P H +P+AD + + ++ V V+MTGMG DG Sbjct: 241 GKNISVIKNSEGLQVVLDNHDTPSRH--KPSADYLFRSVGKLTDYEKVAVIMTGMGSDGT 298 Query: 299 QGIVEIKKRGGI-TIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIR 355 G+ ++ G + IA+ E++ +++GMPKAA++ G++ + +E I ++ V+ R Sbjct: 299 AGLKDMLTAGNVKAIAESEESCVVYGMPKAAVKAGLIHEIKHVEDIAASITSCVKKER 356 >sp|O87717|CHEB_CAUCR PROTEIN-GLUTAMATE METHYLESTERASE >gi|3387366|emb|CAA07180.1| (AJ006687) CheB [Caulobacter crescentus] Length = 344 Score = 265 bits (669), Expect = 8e-70 Identities = 149/343 (43%), Positives = 210/343 (60%), Gaps = 9/343 (2%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIRVLVVDDSA MR ++ +N DP++EV G A D EA M++ PDVVTLD+EMP M Sbjct: 3 KIRVLVVDDSATMRSLISAALNRDPDIEVVGGAGDPFEARGMIKALNPDVVTLDIEMPNM 62 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 NG+D L IM+ P+PV+M+S LTQ GAE T++ALE GA+D + KP+ + +E E Sbjct: 63 NGIDFLEKIMRLRPMPVVMVSTLTQAGAEMTLRALELGAVDCVGKPAD--ATGTQEALAE 120 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPK 186 I+AK+K AA+ R + K +P+ VAI +STGG ++LL I Sbjct: 121 IVAKVKIAARASVR----TNAGAAPTSAPTRRKDFMPSGDIVAIGSSTGGVEALLSILQL 176 Query: 187 FPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGD-YHME 245 FPE + + QHMP FT SFA RLD S V+EA +G ++E GK YVAPG H+E Sbjct: 177 FPETCPPTV-ITQHMPATFTASFAARLDRSSGAKVQEASDGALLEPGKVYVAPGGATHLE 235 Query: 246 VTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIK 305 V G + P + G RP+ D + + A G + VGV++TGMGRDGAQG++ ++ Sbjct: 236 VVRSAGLRCRLVAGDP-VSGHRPSVDVLFNSVAHAVGDKAVGVILTGMGRDGAQGLLTMR 294 Query: 306 KRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVV 348 K G T+ QDE + +++GMP++A E G V+ V L + ++++ Sbjct: 295 KAGAKTLGQDEASCVVYGMPRSAFEIGAVERQVSLSSMGQSIL 337 >sp|Q52883|CHEB_RHIME PROTEIN-GLUTAMATE METHYLESTERASE >gi|2120799|pir||S61837 protein-glutamate methylesterase (EC 3.1.1.61) cheB - Rhizobium meliloti >gi|534840|gb|AAA86677.1| (U13166) CheB [Sinorhizobium meliloti] Length = 349 Score = 262 bits (663), Expect = 4e-69 Identities = 146/350 (41%), Positives = 213/350 (60%), Gaps = 9/350 (2%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVLVVDDSA MR ++ ++N+DP++ V G+A D +EA + +++ PDVVTLD+EMP MNG Sbjct: 6 RVLVVDDSATMRGLISAVLNADPDISVVGQAADALEARQAIKQLDPDVVTLDIEMPNMNG 65 Query: 69 LDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEII 128 L+ L IM+ P+PVIM+S LT GAEATI ALE GA D + KP R+L D Sbjct: 66 LEFLDKIMRLRPMPVIMVSTLTHRGAEATIAALEIGAFDCVGKPQPGDPHPFRDLAD--- 122 Query: 129 AKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKFP 188 K+K AA+ R+ + A V R VAI ASTGG ++L+ + KFP Sbjct: 123 -KVKAAARSQRK--SMITSNRAAAPAATAVSDYRAGRKIVAIGASTGGVEALITVLQKFP 179 Query: 189 ENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAP-GDYHMEVT 247 N + + QHMP FT+SFA+RL+ + V+EA +G +E G+ Y+AP G+ H+EV Sbjct: 180 ANCPPTV-ITQHMPHTFTKSFAERLNRLCAPTVQEATDGARLEVGRVYLAPGGERHLEVH 238 Query: 248 LRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKR 307 P L P ++G RP+ D + + A++ GR +GV++TGMGRDGA G+++++ Sbjct: 239 -NAAAPCCRLVDHPPVNGHRPSVDVLFDSVAELAGRNAIGVILTGMGRDGAAGLLKLRHA 297 Query: 308 GGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIRGG 357 G T Q+EKT +++GMP+ A E G V+ +PL I E +++ + G Sbjct: 298 GARTFGQNEKTCVVYGMPRVAYELGAVETQLPLGSIGEEILKTAAARKEG 347 >sp|O85128|CHEB_AGRTU PROTEIN-GLUTAMATE METHYLESTERASE >gi|3282794|gb|AAC25079.1| (AF044495) CheB homolog [Agrobacterium tumefaciens] Length = 351 Score = 257 bits (649), Expect = 2e-67 Identities = 138/352 (39%), Positives = 216/352 (61%), Gaps = 7/352 (1%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVLVVDDS MR ++ ++ +DPE+EV G+A + +EA +++ PDVVTLD+EMP MNG Sbjct: 6 RVLVVDDSPTMRGLISAVLKADPEVEVVGQAGNAMEARAAIKQLNPDVVTLDIEMPEMNG 65 Query: 69 LDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEII 128 L+ L IM+ P+PVIM+S+LT GA+A++ ALE GA D + KP+ + +L D Sbjct: 66 LEFLEKIMRLRPMPVIMVSSLTHRGADASLAALEIGAFDCVGKPAPGDARPFGDLAD--- 122 Query: 129 AKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKFP 188 K+K AA+ R + + R VAI +STGG ++L+ + KFP Sbjct: 123 -KVKAAARSQHAAYRATRPETAAAPQPVPMSEYRAGRKVVAIGSSTGGVEALIAVLQKFP 181 Query: 189 ENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG-DYHMEVT 247 N ++ QHMPP FT+SFA+RL+ + V+EA +G ++ GK Y+APG + H+++ Sbjct: 182 ANCPPTVI-TQHMPPTFTKSFAERLNRICAPVVEEATDGARLQTGKIYLAPGGERHLQIA 240 Query: 248 LRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKR 307 R L++ P ++G RP+ D + + A++ GR VGV++TGMGRDGA G+++++ Sbjct: 241 NRSAPCCRLLDRDP-VNGHRPSVDVLFDSVAELAGRNAVGVILTGMGRDGAAGLLKMRHA 299 Query: 308 GGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAVEMIRGGGN 359 G T+ Q+EKT +++GMP+ A E G V+ +PL I E +++ + G + Sbjct: 300 GARTVGQNEKTCVVYGMPRVAYELGAVEQQLPLASIGEEILKLTTARKEGAD 351 >gb|AAG19394.1| (AE005033) chemotaxis protein; CheB [Halobacterium sp. NRC-1] Length = 347 Score = 255 bits (644), Expect = 7e-67 Identities = 137/342 (40%), Positives = 203/342 (59%), Gaps = 4/342 (1%) Query: 11 LVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNGLD 70 LVVDDS FMR V+ DI+ D ++V G A +G A++ V +PDV+T+DVEMP M+G++ Sbjct: 5 LVVDDSHFMRTVISDILE-DGGVDVVGTAENGARALDAVTDVQPDVITMDVEMPEMDGIE 63 Query: 71 ALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEIIAK 130 A IM++ P P++M+SALT E A+AT++A+E GAID KP +IS + +E++A Sbjct: 64 ATAEIMREQPTPILMVSALTTEDADATLEAMEKGAIDTFAKPGGTISTELSGHSEELVAA 123 Query: 131 IKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKFPEN 190 ++ A + + + I ASTGGP + I P Sbjct: 124 VERVASADP--TAGHDVEMEPASPPDATTSEYADNPTLLIGASTGGPNVVESILASLPAE 181 Query: 191 LKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVT-LR 249 +L+VQHMP FT FA RLD+ S+ D+ EAE+G + G+ VA GDYHM V+ Sbjct: 182 ADFRVLIVQHMPDQFTSRFADRLDAASQYDITEAEDGSRIGGGEGLVARGDYHMRVSGYS 241 Query: 250 GGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGG 309 G+ + L++ ++H VRPA D +AA+ V VV+TGMG DGA G+ +K GG Sbjct: 242 NGRLRVRLDQSERLHSVRPAIDVTFKSAAERVTDPLVSVVLTGMGSDGADGVRAVKDAGG 301 Query: 310 ITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAV 351 T+AQ+E TS +FG+P+ AIETG VD V+P++++ E + ++ Sbjct: 302 ATLAQNEATSAVFGIPERAIETGCVDDVLPVDQLTEAIADSI 343 >sp|Q48300|CHEB_HALSA PROTEIN-GLUTAMATE METHYLESTERASE >gi|1363463|pir||S58646 protein-glutamate methylesterase (EC 3.1.1.61) cheB [similarity] - Halobacterium salinarum >gi|994803|emb|CAA60162.1| (X86407) cheB [Halobacterium salinarum] Length = 347 Score = 255 bits (644), Expect = 7e-67 Identities = 137/342 (40%), Positives = 203/342 (59%), Gaps = 4/342 (1%) Query: 11 LVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNGLD 70 LVVDDS FMR V+ DI+ D ++V G A +G A++ V +PDV+T+DVEMP M+G++ Sbjct: 5 LVVDDSHFMRTVISDILE-DGGVDVVGTAENGARALDAVTDVQPDVITMDVEMPEMDGIE 63 Query: 71 ALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEIIAK 130 A IM++ P P++M+SALT E A+AT++A+E GAID KP +IS + +E++A Sbjct: 64 ATAEIMREQPTPILMVSALTTEDADATLEAMEKGAIDTFAKPGGTISTELSGHSEELVAA 123 Query: 131 IKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKFPEN 190 ++ A + + + I ASTGGP + I P Sbjct: 124 VERVASADP--TAGHDVEMEPASPPDATTSEYADNPTLLIGASTGGPNVVESILASLPAE 181 Query: 191 LKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVT-LR 249 +L+VQHMP FT FA RLD+ S+ D+ EAE+G + G+ VA GDYHM V+ Sbjct: 182 ADFRVLIVQHMPDQFTSRFADRLDAASQYDITEAEDGSRIGGGEGLVARGDYHMRVSGYS 241 Query: 250 GGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGG 309 G+ + L++ ++H VRPA D +AA+ V VV+TGMG DGA G+ +K GG Sbjct: 242 NGRLRVRLDQSERLHSVRPAIDVTFKSAAERVTDPLVSVVLTGMGSDGADGVRPVKDAGG 301 Query: 310 ITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVRAV 351 T+AQ+E TS +FG+P+ AIETG VD V+P++++ E + ++ Sbjct: 302 ATLAQNEATSAVFGIPERAIETGCVDDVLPVDQLTEAIADSI 343 >pir||H82122 protein-glutamate methylesterase CheB VC2062 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9656608|gb|AAF95208.1| (AE004280) protein-glutamate methylesterase CheB [Vibrio cholerae] Length = 377 Score = 244 bits (617), Expect = 1e-63 Identities = 140/373 (37%), Positives = 213/373 (56%), Gaps = 36/373 (9%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+VLVVDDS+F R+ + +IINS+ LEV A +G EA+E + +PDV+T+D+EMP M+ Sbjct: 3 IKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGKEAVEKAARLKPDVITMDIEMPVMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+ A+R IM PVP++M S+LT +GA+AT+ AL+ GA+DF+PK I+ N +DE Sbjct: 63 GISAVREIMANNPVPILMFSSLTHDGAKATLDALDAGALDFLPKKFEDIARN----RDEA 118 Query: 128 IAKIKE-AAKVPRRFLELRRIRLLR-------------------------------VQKA 155 + +++ + + + LRR R V A Sbjct: 119 VTLLQQRVLSIASKKMFLRRPAAPRPAPTTSIAASSSLSQERAAATSPLGNRPSTAVSAA 178 Query: 156 KKVKPSVPARIAVAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDS 215 + K S AI STGGP +L KI K P N I+L+QHMP FT +FA RL+S Sbjct: 179 TRFKASGKKYQLTAIGTSTGGPVALQKILTKLPVNYPHPIVLIQHMPATFTAAFASRLNS 238 Query: 216 VSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMI 275 + KI+VKEAE+GD++ G AY+APG M + R G + + +P D Sbjct: 239 LCKIEVKEAEDGDMLRPGVAYLAPGGKQMMLDGRPGAARLRIIDGGDRMNYKPCVDVTFG 298 Query: 276 TAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVD 335 +AA++FG + + +++TGMG DG +G +K+ G AQDE++ +++GMP+A + G+ Sbjct: 299 SAAKIFGDKVLSMILTGMGADGREGARMLKQAGATIWAQDEESCVVYGMPQAVAKAGIST 358 Query: 336 YVVPLEKIPETVV 348 +PLE+I E ++ Sbjct: 359 EDLPLERIAERML 371 >gb|AAF32418.1| (AF069392) chemotaxis protein CheB [Vibrio parahaemolyticus] Length = 369 Score = 243 bits (613), Expect = 3e-63 Identities = 137/365 (37%), Positives = 213/365 (57%), Gaps = 28/365 (7%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+VLVVDDS+F R+ + +IINS+ LEV A +G EA+E + +PDV+T+D+EMP M+ Sbjct: 3 IKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGREAVEKAKALKPDVITMDIEMPVMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+ A+R IM P P++M S+LT +GA+AT+ AL+ GA+DF+PK I+ N +DE Sbjct: 63 GITAVREIMAASPTPILMFSSLTHDGAKATLDALDAGALDFLPKKFEDIARN----RDEA 118 Query: 128 IAKIKEAA-KVPRRFLELRRIRLLRVQKAKKVKP----------SVPARIAV-------- 168 ++ +++ ++ + +RR +P S PAR A Sbjct: 119 VSLLQQRVIQIASKRAFMRRPVARPAAATSAARPLASRTAAPAASAPARPAATKFRASGK 178 Query: 169 -----AIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKE 223 AI STGGP +L KI + P N I+L+QHMP FT +FA RL+++ KI VKE Sbjct: 179 KYQLTAIGTSTGGPVALQKILTRLPMNYPHPIVLIQHMPATFTAAFASRLNTLCKIQVKE 238 Query: 224 AEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGR 283 A++GDV++ G AY+APG M + R G + + +P D +AA+V+G Sbjct: 239 AQDGDVLQAGVAYLAPGGKQMMIDGRAGAARLRIIDGGDRMNYKPCVDVTFGSAAKVYGD 298 Query: 284 RTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKI 343 + + +V+TGMG DG +G +K G AQDE++ +++GMP+A + G+ +PL++I Sbjct: 299 KVLSMVLTGMGADGREGARMLKSAGSTIWAQDEESCVVYGMPQAVAKAGISSEDLPLDRI 358 Query: 344 PETVV 348 E ++ Sbjct: 359 AERMM 363 >sp|O52262|CHEB_PSEPU PROTEIN-GLUTAMATE METHYLESTERASE >gi|2853601|gb|AAC08065.1| (AF031898) CheB [Pseudomonas putida] Length = 374 Score = 242 bits (610), Expect = 7e-63 Identities = 135/373 (36%), Positives = 212/373 (56%), Gaps = 33/373 (8%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 ++VLVVDDS F R+ + +I+++DP ++V G A +G EAI+ +PDV+T+D EMP M+ Sbjct: 3 VKVLVVDDSGFFRRRVSEILSADPTIQVVGTATNGKEAIDQALALKPDVITMDYEMPMMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+ A+R IM++ P PV+M S+LT EGA T+ AL+ GA+D++PK IS N ++K + Sbjct: 63 GITAVRHIMQRCPTPVLMFSSLTHEGARVTLDALDAGAVDYLPKNFEDISRNPDKVKQML 122 Query: 128 IAKIKEAAKVPRRFLELRRIRLLRVQ-------------KAKKVKPSVPARIA------- 167 K+ ++ RR R + +P+ AR A Sbjct: 123 CEKVHTISRSNRRLGSYARPAPVAAPVPASSPAPASSFASPAPARPAATARAAAPAASHS 182 Query: 168 ----------VAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVS 217 VAI STGGP +L ++ + P N A I+L+QHMP FT++FA+RLD + Sbjct: 183 PAPKRKPYKLVAIGTSTGGPVALQRVLTQLPANFPAPIVLIQHMPAAFTKAFAERLDKLC 242 Query: 218 KIDVKEAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITA 277 +I+VKEAE+GD++ G A +APG M + RG ++ +++ +P D +A Sbjct: 243 RINVKEAEDGDMLRPGLALLAPGGKQMMIDGRGTVKILPGDER---LNYKPCVDITFGSA 299 Query: 278 AQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYV 337 A+ +G + + VV+TGMG DG +G +K+ G AQDE + +I+GMP A ++ + D V Sbjct: 300 AKSYGDKVLSVVLTGMGADGREGARLLKQGGSTVWAQDEASCVIYGMPMAIVKANLADAV 359 Query: 338 VPLEKIPETVVRA 350 L+ I + +V A Sbjct: 360 YSLDDIGKHLVEA 372 >sp|O87125|CHEB_PSEAE PROTEIN-GLUTAMATE METHYLESTERASE >gi|11262675|pir||T46615 chemotaxis protein cheB [imported] - Pseudomonas aeruginosa >gi|3721571|dbj|BAA33550.1| (AB012767) CheB [Pseudomonas aeruginosa] Length = 368 Score = 241 bits (608), Expect = 1e-62 Identities = 137/369 (37%), Positives = 213/369 (57%), Gaps = 27/369 (7%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 ++VLVVDDS F R+ + +I+++D +++V G A +G EAIE RPDV+T+D EMP M+ Sbjct: 3 VKVLVVDDSGFFRRRVSEILSADGQIQVVGTATNGREAIEQALALRPDVITMDYEMPLMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+ A+R IM++ P PV+M S+LT EGA T+ AL+ GA+D++PK IS N +++ + Sbjct: 63 GITAVRNIMQRCPTPVLMFSSLTHEGARVTLDALDAGAVDYLPKNFEDISRNPDKVRQLL 122 Query: 128 IAKIKEAAKVPRRFLELRRI-----------------RLLRVQKAKKVKP----SVPARI 166 K+ A+ RR + L + RV P + P R Sbjct: 123 CEKVLTIARSNRRSISLPPLPSATSSSHAPASSSSVGASARVGAGASPAPASTSAAPKRK 182 Query: 167 A---VAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKE 223 A VAI STGGP +L ++ + P N A ++L+QHMP FT++FA+RLD + +I+VKE Sbjct: 183 AYRLVAIGTSTGGPVALQRVLTQLPANFPAPLVLIQHMPAAFTKAFAERLDKLCRINVKE 242 Query: 224 AEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGR 283 AE+GD++ G A +APG M V RG ++ +++ +P D +AA+ + Sbjct: 243 AEDGDILRPGLALLAPGGKQMMVDGRGTVRILPGDER---LNYKPCVDVTFGSAAKAYND 299 Query: 284 RTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKI 343 + + VV+TGMG DG +G +K+ G AQDE + +I+GMP A ++ + D V L+ I Sbjct: 300 KVLAVVLTGMGADGREGARLLKQGGSQVWAQDEASCVIYGMPMAVVKANLADAVYGLDDI 359 Query: 344 PETVVRAVE 352 +V A + Sbjct: 360 GRHLVEACQ 368 >pir||G83463 probable methyltransferase PA1459 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9947411|gb|AAG04848.1|AE004575_7 (AE004575) probable methyltransferase [Pseudomonas aeruginosa] Length = 368 Score = 239 bits (604), Expect = 3e-62 Identities = 136/369 (36%), Positives = 212/369 (56%), Gaps = 27/369 (7%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 ++VLVVDDS F R+ + +I+++D +++V G +G EAIE RPDV+T+D EMP M+ Sbjct: 3 VKVLVVDDSGFFRRRVSEILSADGQIQVVGTGTNGREAIEQALALRPDVITMDYEMPLMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+ A+R IM++ P PV+M S+LT EGA T+ AL+ GA+D++PK IS N +++ + Sbjct: 63 GITAVRNIMQRCPTPVLMFSSLTHEGARVTLDALDAGAVDYLPKNFEDISRNPDKVRQLL 122 Query: 128 IAKIKEAAKVPRRFLELRRI-----------------RLLRVQKAKKVKP----SVPARI 166 K+ A+ RR + L + RV P + P R Sbjct: 123 CEKVLTIARSNRRSISLPPLPSATSSSHAPASSSSVGASARVGAGASPAPASTSAAPKRK 182 Query: 167 A---VAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKE 223 A VAI STGGP +L ++ + P N A ++L+QHMP FT++FA+RLD + +I+VKE Sbjct: 183 AYRLVAIGTSTGGPVALQRVLTQLPANFPAPLVLIQHMPAAFTKAFAERLDKLCRINVKE 242 Query: 224 AEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGR 283 AE+GD++ G A +APG M V RG ++ +++ +P D +AA+ + Sbjct: 243 AEDGDILRPGLALLAPGGKQMMVDGRGTVRILPGDER---LNYKPCVDVTFGSAAKAYND 299 Query: 284 RTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKI 343 + + VV+TGMG DG +G +K+ G AQDE + +I+GMP A ++ + D V L+ I Sbjct: 300 KVLAVVLTGMGADGREGARLLKQGGSQVWAQDEASCVIYGMPMAVVKANLADAVYGLDDI 359 Query: 344 PETVVRAVE 352 +V A + Sbjct: 360 GRHLVEACQ 368 >gb|AAF67048.1|AF183382_8 (AF183382) methylesterase CheB [Pseudomonas putida] Length = 374 Score = 238 bits (601), Expect = 8e-62 Identities = 134/373 (35%), Positives = 210/373 (55%), Gaps = 33/373 (8%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 ++VLVVDDS F R+ + +I+++DP ++V G A +G EAI+ +PDV+T+D EMP M+ Sbjct: 3 VKVLVVDDSGFFRRRVSEILSADPTIQVVGTATNGKEAIDQALALKPDVITMDYEMPMMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+ A+R IM++ P PV+M S+LT EGA T+ AL+ GA+D++PK IS N ++K + Sbjct: 63 GITAVRHIMQRCPTPVLMFSSLTHEGARVTLDALDAGAVDYLPKNFEDISRNPDKVKQML 122 Query: 128 IAKIKEAAKVPRRF-------LELRRIRLLRVQKAKKVKPSVPARIA------------- 167 K+ ++ RR R + A PAR A Sbjct: 123 CEKVHTISRSNRRLGSYALAGAGRRPVPASSPAPASSFASPAPARPAATARAAAPAASHS 182 Query: 168 ----------VAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVS 217 + I STGGP +L ++ + P N A I+L+QHMP FT++FA+RLD + Sbjct: 183 PAPKRKPYKLLRIGTSTGGPVALQRVLTQLPANFPAPIVLIQHMPAAFTKAFAERLDKLC 242 Query: 218 KIDVKEAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITA 277 +I+VKEAE+GD++ G A +APG M + RG ++ +++ +P D +A Sbjct: 243 RINVKEAEDGDMLRPGLALLAPGGKQMMIDGRGTVKILPGDER---LNYKPCVDITFGSA 299 Query: 278 AQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYV 337 A+ +G + + VV+TGMG DG +G +K+ G AQDE + +I+GMP A ++ + D V Sbjct: 300 AKSYGDKVLSVVLTGMGADGREGARLLKQGGSTVWAQDEASCVIYGMPMAIVKANLADAV 359 Query: 338 VPLEKIPETVVRA 350 L+ I + +V A Sbjct: 360 YSLDDIGKHLVEA 372 >sp|O83639|CHEB_TREPA PROTEIN-GLUTAMATE METHYLESTERASE >gi|7435068|pir||E71300 probable protein-glutamate methylesterase (cheB) - syphilis spirochete >gi|3322930|gb|AAC65606.1| (AE001238) protein-glutamate methylesterase (cheB) [Treponema pallidum] Length = 403 Score = 235 bits (594), Expect = 5e-61 Identities = 140/392 (35%), Positives = 208/392 (52%), Gaps = 53/392 (13%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I VL+VDDSA MRKV+ +I P L + G+A +G A++M+++ +PDV+ LD+EMP MN Sbjct: 7 IAVLIVDDSALMRKVIGKVIEGAPGLSIAGKAMNGRFALDMLERVQPDVILLDLEMPHMN 66 Query: 68 GLDALRVIMK-KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 GL L + + +PVI++S++ +EGA T++ LE GA DF+ KP S S ++R + + Sbjct: 67 GLQFLEQRKRLRIDIPVIILSSIAKEGARVTMQCLELGASDFVTKPFGSESAHLRTVSRK 126 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPS------------------------- 161 I+ + RR+ LRR R +P+ Sbjct: 127 IVDYVTAYG---RRYKLLRRTRRCLAMDTPVERPAGEEDLNCLDTQERASLPSVCARAPA 183 Query: 162 -------------------VPARIA-----VAIAASTGGPQSLLKIFPKFPENLKAGILL 197 VP R + +AI STGGP +L IF + +L +++ Sbjct: 184 RDRASYTITPTEGARQTRIVPLRESGALQIIAIGVSTGGPSALRHIFAQLDADLPQPVVV 243 Query: 198 VQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITL 257 VQHMP GFTR FA L+ V ++VKEA+EGD+V G+ +APGD H+ V R V + Sbjct: 244 VQHMPAGFTREFAYSLNQVCALEVKEAQEGDLVRRGRVLIAPGDRHLTVERRSLATVAHI 303 Query: 258 NKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEK 317 N +G RP+ D + + A+ F R +G++MTGMGRDGA + + G TIAQD Sbjct: 304 NSDEPENGHRPSVDVLFESVARHFENRALGILMTGMGRDGAAQLARLYTEGSRTIAQDAD 363 Query: 318 TSIIFGMPKAAIETGMVDYVVPLEKIPETVVR 349 + I++GMP+ A E G V V L+ + T+ R Sbjct: 364 SCIVYGMPRVACELGAVGEQVSLDDMAATINR 395 >sp|P72253|CHEB_RHOCE PROTEIN-GLUTAMATE METHYLESTERASE >gi|1621288|gb|AAB71332.1| (U64519) CheB [Rhodospirillum centenum (Rhodocista centenaria)] Length = 394 Score = 216 bits (545), Expect = 3e-55 Identities = 126/362 (34%), Positives = 195/362 (53%), Gaps = 28/362 (7%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RV+VVDDSA +R ++ + SDPE++V +G AI + + DV+ LD+EMP ++G Sbjct: 21 RVMVVDDSAVIRGLITRALESDPEIKVVASVANGQMAINTLSRQPIDVIVLDIEMPVLDG 80 Query: 69 LDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRE-LKDE 126 L AL +++ P V ++M S LT +GA+ +++AL GA D+IPKPSS+ + + K E Sbjct: 81 LSALPHLLQADPNVKIVMASTLTAKGADISLRALRAGAADYIPKPSSTRELTGADTFKRE 140 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPS----VPARI---------------- 166 + K+K RR R +P+ +P+ + Sbjct: 141 LTEKVKALGAAARRSGPRREGTAAARPPGAAAQPTSGYTLPSPVRAKPETGPLTVRPLPP 200 Query: 167 -----AVAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDV 221 +AI +STGGPQ+L ++ IL+ QHMP FT A + I Sbjct: 201 DGRPDVIAIGSSTGGPQALFEVLGHL-RGATQPILITQHMPATFTTILADHITRQCGIQC 259 Query: 222 KEAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVF 281 EA++G+ + G+AYVAPGD+H V R G P + L K + RPA DPM+ + + + Sbjct: 260 AEAKDGEPIVGGRAYVAPGDFHFLVVNRNGVPTVQLTKDAPENFCRPAVDPMLRSIVRQW 319 Query: 282 GRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLE 341 GRR + V++TGMG DG +G + + GG+ I QDE TS+++GMP A G+ ++PL+ Sbjct: 320 GRRVLSVILTGMGHDGQKGCESVVQAGGVVIGQDEATSVVWGMPGAVATAGLCSAILPLK 379 Query: 342 KI 343 +I Sbjct: 380 EI 381 >sp|O33558|CHEB_RHOSH PROTEIN-GLUTAMATE METHYLESTERASE >gi|11262673|pir||T45021 protein-glutamate methylesterase (EC 3.1.1.61) cheB [imported] - Rhodobacter sphaeroides >gi|2293004|emb|CAA04433.1| (AJ000977) methylesterase [Rhodobacter sphaeroides] Length = 369 Score = 215 bits (541), Expect = 8e-55 Identities = 129/348 (37%), Positives = 199/348 (57%), Gaps = 9/348 (2%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVL+VDDSA R + K I+ SDP L+V D A ++ PDV+ LD+E+P M+G Sbjct: 11 RVLIVDDSAAARAMFKVIVESDPALQVMAAVPDAFAAARAMRTELPDVILLDLELPSMDG 70 Query: 69 LDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEII 128 L LR IM+++P+PV++ S+ G EA + ALE GA + I KP++ + +E I Sbjct: 71 LTFLRKIMQQHPIPVVVCSSHVGAGTEAMVSALELGAREVISKPAARNDLERQEASIRIC 130 Query: 129 AKIKEAAKVPRRFLELRRIRLLRVQK--AKKVKPSVPAR------IAVAIAASTGGPQSL 180 I+ A + RR + L K A ++ P+ P R V I ASTGG ++L Sbjct: 131 DAIRAATETTRRRSQPEPRPLAPGPKLTADEILPARPPRPVPETMPVVCIGASTGGTEAL 190 Query: 181 LKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPG 240 + P + I++VQHMP GFT +FA+RLDS+ I+V EAE+ V G+A +A G Sbjct: 191 RDVLTALPASAPP-IVIVQHMPRGFTAAFARRLDSLCAIEVLEAEDEMQVMPGRAIIAQG 249 Query: 241 DYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQG 300 D H+ + R +++ + RP+ D + +AAQ G +GV+MTGMG DGA+ Sbjct: 250 DRHLLLRRRNQGYRVSVLDGAYVCRHRPSVDVLFRSAAQEAGGNALGVIMTGMGDDGARC 309 Query: 301 IVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVV 348 + E++ G TIAQ+E++ +++GMP+ A+ G V V PL+++ ++ Sbjct: 310 MAEMRAAGAETIAQNEESCVVYGMPREAVAHGGVGKVEPLDRLAARIM 357 >pir||A82206 protein-glutamate methylesterase CheB VC1401 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9655896|gb|AAF94558.1| (AE004218) protein-glutamate methylesterase CheB [Vibrio cholerae] Length = 358 Score = 200 bits (503), Expect = 2e-50 Identities = 117/327 (35%), Positives = 186/327 (56%), Gaps = 6/327 (1%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +++LVVDDSA MR + DI+ + P E+ ARDG++AI+ V K +PDV+TLD+ MP M+ Sbjct: 1 MKILVVDDSALMRTTISDILQNIPNAEI-KTARDGMDAIDKVMKWQPDVMTLDINMPNMD 59 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 GL L IM + P+P++M+S+LT EGA T++AL GA+DF+ KP +I ++E+ I Sbjct: 60 GLTCLTQIMVERPLPIVMLSSLTHEGAITTLEALYLGAVDFVAKPGGTICGRLQEVAPLI 119 Query: 128 IAKIKEAAKVPRRF---LELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIF 184 K++ AAK+ + L + + K+ V+ + ++A+ + STGGP ++ I Sbjct: 120 REKVRAAAKMRVKVHTQLSVSPAEVEHKHKSPVVENNEDHQLAI-MGVSTGGPGTVETIL 178 Query: 185 PKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHM 244 P + I++ QHMP FT +FA+RL+ + VKE V+E G YV GD Sbjct: 179 RHLPADFSLPIIVNQHMPESFTAAFAQRLNRHLQQPVKEINRALVLEAGTVYVCKGDRDC 238 Query: 245 EVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGR-RTVGVVMTGMGRDGAQGIVE 303 VTLR GK P+ ++ +A + G + V++TGMG DGA + + Sbjct: 239 IVTLRDGKLAAMPTPNDSHFSWHPSVSKLVASAIRTCGEDNLLCVMLTGMGDDGATEMAQ 298 Query: 304 IKKRGGITIAQDEKTSIIFGMPKAAIE 330 + + GI AQ+ T ++ MP+A ++ Sbjct: 299 VAQGNGIVFAQEPTTCVVPSMPEALLK 325 >sp|O51376|Y415_BORBU PROBABLE PROTEIN-GLUTAMATE METHYLESTERASE BB0415 >gi|7435071|pir||F70151 protein-glutamate methylesterase (cheB-1) homolog - Lyme disease spirochete >gi|2688322|gb|AAC66789.1| (AE001146) protein-glutamate methylesterase (cheB-1) [Borrelia burgdorferi] Length = 375 Score = 178 bits (448), Expect = 7e-44 Identities = 113/362 (31%), Positives = 196/362 (53%), Gaps = 32/362 (8%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI VL+++ A RK++ D+INS P+L+V A + A ++K +P+V+ +++E + Sbjct: 4 KISVLIIEYFAVKRKLISDLINSSPKLQVIATASNSKFATNKLKKHKPEVILMNLEENNI 63 Query: 67 NG---LDALRVIMKKYPVPVI-----MISALTQEGAEATI------------------KA 100 L+ + K P+ V +I+ +GA+ I Sbjct: 64 KDILFLEEKNSLNKTIPIVVTSSNQNLINIAASKGADDLILVSKNKKSHEIKKEQIINSL 123 Query: 101 LEYGAIDFIPKPSSSISINMREL-KDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVK 159 L YG+I K + + + + + I K + E + + L ++ KK+K Sbjct: 124 LAYGSISIKNKIACNKDMKTKNYERANFILNHKNDISSLNQLEEHAKEKTLNEKEIKKLK 183 Query: 160 PSVPARIAVAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKI 219 + +AI S GGP +L I P+ PE+ I++VQHMP GFT FAK L+++ KI Sbjct: 184 --LRKFDIIAIGVSAGGPVALKSILPEIPESFPP-IIIVQHMPKGFTEEFAKNLNNLCKI 240 Query: 220 DVKEAEEGDVVEEGKAYVAPGDYHMEV-TLRGGKPVITLNKKPKMHGVRPAADPMMITAA 278 VKE +++++G AY++ G YH ++ + G + TL+ K ++G +P+ + + A Sbjct: 241 SVKETTNNEILKQGYAYISSGGYHTKIKKIDGNYQIKTLDGK-HINGHKPSIGVLFQSIA 299 Query: 279 QVFGRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVV 338 ++ + + ++MTGMG DG++ I +IKK GG+TIAQD+++S++FGMPK AI+ +DY+V Sbjct: 300 EIAKDKAIAIIMTGMGNDGSREIGDIKKAGGLTIAQDKESSMVFGMPKIAIKENNIDYIV 359 Query: 339 PL 340 PL Sbjct: 360 PL 361 >sp|P31758|FRZG_MYXXA PROTEIN-GLUTAMATE METHYLESTERASE FRZG >gi|94529|pir||A36707 protein-glutamate methylesterase homolog - Myxococcus xanthus >gi|150093|gb|AAA25397.1| (M35200) FrzG protein [Myxococcus xanthus] Length = 334 Score = 169 bits (425), Expect = 3e-41 Identities = 105/311 (33%), Positives = 174/311 (55%), Gaps = 18/311 (5%) Query: 45 AIEMVQKCRPDVVTLDVEMPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYG 104 A+ VQ+ PDVV +D+ ++ L A+ +M + PVPV+ + G EA +A+ G Sbjct: 39 ALVAVQRHFPDVVLVDLSA--LDALPAIEHVMVERPVPVLALHPGVLSGQEA-FQAMVAG 95 Query: 105 AIDFIPKPSSSISINMRELKDEII--AKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSV 162 A+D + +P++ + +++ A++K +V R Q A++ KP Sbjct: 96 ALDVLERPANPGPEFWTHVSRKLVLLAQVKAVRQVQTR---------PPPQAAREAKPPP 146 Query: 163 PARIAVAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVK 222 P + VAIAAS GGP+++ ++ P A I QH+ GFT A L + + + V Sbjct: 147 PYPL-VAIAASLGGPKAVAQVLRMIPRAFPAPIAYCQHISDGFTEGLAHWLSNETALRVL 205 Query: 223 EAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFG 282 EAE ++ G Y+AP H+ V G + L+ P + G RP+ D ++ +A + FG Sbjct: 206 EAEHDVLMAPGTVYIAPSGSHLLVRPEGR---LELDAGPALRGFRPSCDMLLTSAGESFG 262 Query: 283 RRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEK 342 R +GV++TGMGRDGA+G+ EI++RGG TIAQDE +S+++GMP+ A+ G V+PL + Sbjct: 263 PRCIGVILTGMGRDGARGLKEIRERGGRTIAQDEASSVVWGMPREAVLMGAAHEVLPLSR 322 Query: 343 IPETVVRAVEM 353 I +++ V++ Sbjct: 323 IGAALMQWVDV 333 >pir||F83183 probable methylesterase PA3703 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9949867|gb|AAG07090.1|AE004789_10 (AE004789) probable methylesterase [Pseudomonas aeruginosa] Length = 335 Score = 166 bits (415), Expect = 5e-40 Identities = 106/336 (31%), Positives = 180/336 (53%), Gaps = 10/336 (2%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +R+ +V+D + L+ + +P+ ++ A +G EA+ PDVV +D+ MP M+ Sbjct: 1 MRIGIVNDMPLAVEALRRALAFEPQHQIVWVASNGAEAVTQCAADTPDVVLMDLLMPVMD 60 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G++A R IM + P +++++ ++ +A+ YGA+D + P+ I N + + Sbjct: 61 GVEATRRIMAESPCAIVIVTVDIEQNVHRVFEAMGYGALDAVNTPALGIG-NPQTAAAPL 119 Query: 128 IAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTGGPQSLLKIFPKF 187 + KI+ +L +R +VQ R+ VAI AS GGP SL + + Sbjct: 120 LRKIQNVG-----WLIGQRDNRGKVQVVPPKAGGARQRL-VAIGASAGGPASLAVLLKQL 173 Query: 188 PENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVT 247 P + A ++LVQH+ F A+ L S SK+ V+ A +G+ G+ +A + H+ + Sbjct: 174 PASFNAAVVLVQHVDEVFAAGMAEWLASESKLPVRLARDGEPPIPGQILLAGTNNHIRL- 232 Query: 248 LRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKR 307 LR G V T +P+ RP+ D + A + VGV++TGMGRDGAQG+ ++++R Sbjct: 233 LRNGSLVYTA--EPRSFVYRPSIDVFFESVANYWRGDAVGVLLTGMGRDGAQGLKQMRER 290 Query: 308 GGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKI 343 G +TIAQD+ + ++GMPKAA ++ LEKI Sbjct: 291 GFLTIAQDQASCAVYGMPKAAAAIDAAVQILSLEKI 326 >sp|Q45047|CHEB_BORBU PROTEIN-GLUTAMATE METHYLESTERASE >gi|7435067|pir||G70170 protein-glutamate methylesterase (cheB-2) homolog - Lyme disease spirochete >gi|2688489|gb|AAC66934.1| (AE001158) protein-glutamate methylesterase (cheB-2) [Borrelia burgdorferi] Length = 385 Score = 164 bits (410), Expect = 2e-39 Identities = 111/384 (28%), Positives = 196/384 (50%), Gaps = 43/384 (11%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +++LV+D +++V + D ++E+ + + I + + PD+V +D R N Sbjct: 1 MKILVIDIQGLIKQVFVRAFSKDNDVEILNAGFNSLNLINVFLQKFPDLVIIDENTARSN 60 Query: 68 GLDALRVIMKKYPVPVIMISA-----------LTQEGAEATIKALEYG-AID-FIPKPSS 114 ++L ++ +PV+ I+ ++E + I L + +D F K + Sbjct: 61 FGNSLNNVLNNISLPVVFIAQNEMLPNFGCLEQSKEKVKLIINKLNFKLTVDLFRSKYLA 120 Query: 115 SISINMREL-KDEIIAKIK----------EAAKVPRRFLELRRIRLLR-------VQKAK 156 I + ++ L K+++I+ + +KV R L + + + A Sbjct: 121 LIKLELKNLGKNKLISSFEVKRIQAPDFSSNSKVELRENNLNDSSIRKSYRVSDVINFAP 180 Query: 157 KVKPSVPARIA-----------VAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGF 205 K P V + + + +STGG ++L +IF + + I++VQHMP GF Sbjct: 181 KNDPDVIIKYQGLINKHKTGKIIVVGSSTGGTEAL-RIFLRSFKKDSPPIIIVQHMPGGF 239 Query: 206 TRSFAKRLDSVSKIDVKEAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHG 265 T+SFAK L++ ID+KEAE+GD++ G +A G YH+ V G + L P + Sbjct: 240 TKSFAKNLNNEFNIDIKEAEDGDILRPGLVIIANGSYHLIVKYSSGNYFVNLLDGPLVSR 299 Query: 266 VRPAADPMMITAAQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMP 325 +P+ + + +AA G +GV++TGMG DGA ++E+KK G TIAQD++TS++FGMP Sbjct: 300 HKPSVNVLFRSAAMYAGSNAIGVILTGMGDDGAVCMLEMKKNGAYTIAQDQETSVVFGMP 359 Query: 326 KAAIETGMVDYVVPLEKIPETVVR 349 AI+ G VD ++PL +I + V+R Sbjct: 360 MEAIKIGAVDKILPLSEIADHVLR 383 >pdb|1CHD| Cheb Methylesterase Domain Length = 203 Score = 159 bits (398), Expect = 5e-38 Identities = 80/195 (41%), Positives = 124/195 (63%), Gaps = 1/195 (0%) Query: 157 KVKPSVPARIAVAIAASTGGPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSV 216 K P + + +AI ASTGG +++ + P + A +++ QHMPPGFTRSFA+RL+ + Sbjct: 2 KAGPLLSSEKLIAIGASTGGTEAIRHVLQPLPLSSPA-VIITQHMPPGFTRSFAERLNKL 60 Query: 217 SKIDVKEAEEGDVVEEGKAYVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMIT 276 +I VKEAE+G+ V G AY+APGD HME+ G I ++ P ++ RP+ D + + Sbjct: 61 CQISVKEAEDGERVLPGHAYIAPGDKHMELARSGANYQIKIHDGPPVNRHRPSVDVLFHS 120 Query: 277 AAQVFGRRTVGVVMTGMGRDGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDY 336 A+ GR VGV++TGMG DGA G++ + + G TIAQ+E + ++FGMP+ AI G V Sbjct: 121 VAKHAGRNAVGVILTGMGNDGAAGMLAMYQAGAWTIAQNEASCVVFGMPREAINMGGVSE 180 Query: 337 VVPLEKIPETVVRAV 351 VV L ++ + ++ + Sbjct: 181 VVDLSQVSQQMLAKI 195 >sp|Q56312|CHEY_THEMA CHEMOTAXIS PROTEIN CHEY >gi|7443033|pir||B72346 chemotaxis response regulator CheY - Thermotoga maritima (strain MSB8) >gi|2781289|pdb|1TMY| Chey From Thermotoga Maritima (Apo-I) >gi|2781153|pdb|3TMY|A Chain A, Chey From Thermotoga Maritima (Mn-Iii) >gi|2781154|pdb|3TMY|B Chain B, Chey From Thermotoga Maritima (Mn-Iii) >gi|2781128|pdb|2TMY| Chey From Thermotoga Maritima (Apo-Ii) >gi|2781158|pdb|4TMY|A Chain A, Chey From Thermotoga Maritima (Mg-Iv) >gi|2781159|pdb|4TMY|B Chain B, Chey From Thermotoga Maritima (Mg-Iv) >gi|940149|gb|AAA96389.1| (U30501) CheY [Thermotoga maritima] >gi|4981223|gb|AAD35782.1|AE001741_15 (AE001741) chemotaxis response regulator CheY [Thermotoga maritima] Length = 120 Score = 96.3 bits (236), Expect = 5e-19 Identities = 53/105 (50%), Positives = 74/105 (70%), Gaps = 4/105 (3%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVL+VDD+AFMR +LKDII EV GEA +G EA+E ++ +PD+VT+D+ MP MNG Sbjct: 4 RVLIVDDAAFMRMMLKDIITK-AGYEVAGEATNGREAVEKYKELKPDIVTMDITMPEMNG 62 Query: 69 LDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 +DA++ IMK P +I+ SA+ Q+ I+A++ GA DFI KP Sbjct: 63 IDAIKEIMKIDPNAKIIVCSAMGQQA--MVIEAIKAGAKDFIVKP 105 >emb|CAA53764.1| (X76170) cheY [Listeria monocytogenes] Length = 119 Score = 95.2 bits (233), Expect = 1e-18 Identities = 53/130 (40%), Positives = 84/130 (63%), Gaps = 13/130 (10%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +++L+VDD+ FMR ++K+I+ D + EV EA +G+EA++ + +PD+VTLD+ MP M+ Sbjct: 2 LKLLIVDDAMFMRTMIKNIVK-DSDFEVVAEAENGLEAVKKYDEVKPDIVTLDITMPEMD 60 Query: 68 GLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 GL+AL IM K P VIM SA+ Q+G + A++ GA DFI KP + D Sbjct: 61 GLEALAQIMAKDPSAKVIMCSAMGQQG--MVVDAIKKGAKDFIVKPFQA---------DR 109 Query: 127 IIAKIKEAAK 136 ++ +++AAK Sbjct: 110 VLEALEKAAK 119 >gi|11498647 chemotaxis response regulator (cheY) [Archaeoglobus fulgidus] >gi|7443007|pir||B69380 chemotaxis response regulator (cheY) homolog - Archaeoglobus fulgidus >gi|2649557|gb|AAB90207.1| (AE001031) chemotaxis response regulator (cheY) [Archaeoglobus fulgidus] Length = 121 Score = 87.4 bits (213), Expect = 2e-16 Identities = 49/117 (41%), Positives = 80/117 (67%), Gaps = 5/117 (4%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 +VL+VDD+AFMRK+L++I+ S ++ GEA +G +A+EM ++ +PD+VT+D+ MP MNG Sbjct: 3 KVLIVDDTAFMRKLLRNILFSGG-FDIAGEAENGKQAVEMYKQLKPDIVTMDIVMPEMNG 61 Query: 69 LDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELK 124 ++AL+ I K P V+M +A+ QE + A++ GA +I KP + + + ELK Sbjct: 62 IEALKAIKKIDPSAKVVMCTAVGQE--QMVKAAIKLGAKGYIVKPFQAPKV-IEELK 115 >gb|AAC27631.1| (AF076485) defective in fruiting DifD [Myxococcus xanthus] Length = 122 Score = 86.6 bits (211), Expect = 4e-16 Identities = 51/105 (48%), Positives = 70/105 (66%), Gaps = 4/105 (3%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVLVVDD+ FMR ++KDI S EV GEA +G+EA+E ++ +PD+ T+D+ MP +G Sbjct: 4 RVLVVDDAIFMRNMIKDIFASGG-FEVVGEAANGLEAVEKYKELKPDLTTMDIVMPFKSG 62 Query: 69 LDALRVIMK-KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 ++A R I+K VIM SAL QE ++A+E GA DFI KP Sbjct: 63 IEATREIIKADSSAVVIMCSALGQE--SLVMEAIEAGASDFIVKP 105 >pir||E71160 probable chemotaxis protein (cheY) - Pyrococcus horikoshii >gi|3256887|dbj|BAA29570.1| (AP000002) 120aa long hypothetical chemotaxis protein (cheY) [Pyrococcus horikoshii] Length = 120 Score = 85.4 bits (208), Expect = 9e-16 Identities = 50/105 (47%), Positives = 72/105 (67%), Gaps = 4/105 (3%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVLVVDD+AFMR +LK I+ EV GEA +G EA+E ++ +PD+VT+D+ MP M+G Sbjct: 3 RVLVVDDAAFMRMLLKKILTQAGH-EVVGEASNGKEAVEKYKQLKPDLVTMDIVMPEMDG 61 Query: 69 LDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 + A++ IMK P +IMI+A+ QE ++AL+ GA +I KP Sbjct: 62 ITAVKEIMKIDPNAKIIMITAVGQEA--KVMEALKSGAKGYIVKP 104 >pir||F75002 chemotaxis response regulator (chey) PAB1330 - Pyrococcus abyssi (strain Orsay) >gi|5458974|emb|CAB50460.1| (AJ248288) chemotaxis response regulator (cheY) [Pyrococcus abyssi] Length = 120 Score = 85.0 bits (207), Expect = 1e-15 Identities = 49/105 (46%), Positives = 72/105 (67%), Gaps = 4/105 (3%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 R+LVVDD+AFMR +LK I+ EV GEA +G EA+E ++ +PD+VT+D+ MP M+G Sbjct: 3 RILVVDDAAFMRMLLKKILTQAGH-EVVGEASNGKEAVEKYKQLKPDLVTMDIVMPEMDG 61 Query: 69 LDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 + A++ IMK P +IMI+A+ QE ++AL+ GA +I KP Sbjct: 62 ITAVKEIMKIDPNAKIIMITAVGQEA--KVMEALKSGAKGYIVKP 104 >dbj|BAB06687.1| (AP001517) two-component response regulator involved in modulation of flagellar [Bacillus halodurans] Length = 120 Score = 83.9 bits (204), Expect = 3e-15 Identities = 50/105 (47%), Positives = 71/105 (67%), Gaps = 6/105 (5%) Query: 10 VLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNGL 69 VLVVDD+AFMR ++KDI+ + + EV GEA +G EA+++ ++ PD+VTLD+ MP +G+ Sbjct: 4 VLVVDDAAFMRMMIKDILTKN-DFEVVGEAANGEEAVQLYKEHSPDLVTLDITMPEKDGI 62 Query: 70 DALRVIMKKY--PVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 AL+ I K Y VIM SA+ Q+ I A++ GA DFI KP Sbjct: 63 QALKEI-KAYDADAKVIMCSAMGQQA--MVIDAIQSGAKDFIVKP 104 >dbj|BAB06163.1| (AP001515) two-component response regulator involved in modulation of flagellar [Bacillus halodurans] Length = 121 Score = 81.5 bits (198), Expect = 1e-14 Identities = 46/105 (43%), Positives = 69/105 (64%), Gaps = 4/105 (3%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 R+L+VDD++FMR ++KDI+ + +V GEA DG +A+E ++ PD+VT+D+ MP +G Sbjct: 4 RILIVDDASFMRMMIKDILTKNG-YDVVGEAHDGEQAVEKYKELSPDLVTMDITMPEKDG 62 Query: 69 LDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 + AL+ I P VIM SA+ Q+ I A++ GA DFI KP Sbjct: 63 IAALKDIRAIDPNAKVIMCSAMGQQA--MVIDAIQAGAKDFIVKP 105 >sp|P45709|CCDB_BACSU CCDB PROTEIN >gi|1361404|pir||S57405 response regulator homolog yneI - Bacillus subtilis >gi|870926|emb|CAA61117.1| (X87845) response regulator [Bacillus subtilis] >gi|1405450|emb|CAA97595.1| (Z73234) YneI [Bacillus subtilis] >gi|2634178|emb|CAB13678.1| (Z99113) alternate gene name: yoxH~similar to two-component response regulator (CheY homolog) [Bacillus subtilis] Length = 120 Score = 81.1 bits (197), Expect = 2e-14 Identities = 47/105 (44%), Positives = 68/105 (64%), Gaps = 4/105 (3%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVLVVDD+ FMR +++I+ + + GEA DG +A ++ +K RPD+VT+D+ MP NG Sbjct: 3 RVLVVDDAKFMRVKIREILE-EANYIIAGEAADGEQAADLYKKLRPDLVTMDITMPVKNG 61 Query: 69 LDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 + ALR I+ P VIM +A+ Q+ +A+E GA DFI KP Sbjct: 62 IKALRDILTFDPKAKVIMCTAMRQQ--RIVTEAIELGAKDFIVKP 104 >emb|CAB99140.1| (AL390188) putative two-component system response regulator [Streptomyces coelicolor A3(2)] Length = 220 Score = 81.1 bits (197), Expect = 2e-14 Identities = 39/104 (37%), Positives = 64/104 (61%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +RVL+VDD +R L+ ++ + P++EV GEA DG + +V++ RPDVV +DV MP ++ Sbjct: 3 VRVLLVDDEPLVRAGLRAVLEAQPDIEVVGEAADGAAVVPLVRQVRPDVVAMDVRMPLLD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 G++A R +++ P ++ T E E +AL GA F+ K Sbjct: 63 GIEATRALLRTVADPPKILVVTTFENDEYVYEALRAGADGFLLK 106 >sp|P24072|CHEY_BACSU CHEMOTAXIS PROTEIN CHEY HOMOLOG >gi|98251|pir||A40874 chemotactic tumbling protein cheY - Bacillus subtilis >gi|142682|gb|AAA22311.1| (M59781) chemotactic response protein [Bacillus subtilis] >gi|2634005|emb|CAB13506.1| (Z99112) two-component response regulator [Bacillus subtilis] Length = 120 Score = 80.0 bits (194), Expect = 4e-14 Identities = 45/105 (42%), Positives = 69/105 (64%), Gaps = 4/105 (3%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 R+L+VDD+AFMR ++KDI+ + EV EA +G +A+E ++ PD+VT+D+ MP M+G Sbjct: 4 RILIVDDAAFMRMMIKDILVKNG-FEVVAEAENGAQAVEKYKEHSPDLVTMDITMPEMDG 62 Query: 69 LDALRVIMK-KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 + AL+ I + +IM SA+ Q+ I A++ GA DFI KP Sbjct: 63 ITALKEIKQIDAQARIIMCSAMGQQ--SMVIDAIQAGAKDFIVKP 105 >sp|P54662|DEGU_BACBR TRANSCRIPTIONAL REGULATORY PROTEIN DEGU >gi|2119837|pir||I39835 transcription activator - Bacillus brevis >gi|710496|gb|AAC41439.1| (L15444) transcriptional activator protein [Brevibacillus brevis] Length = 236 Score = 78.0 bits (189), Expect = 2e-13 Identities = 34/105 (32%), Positives = 68/105 (64%), Gaps = 1/105 (0%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI+++++DD R+ +K I+ +PE EV + DG A+E+V+K PDV+ +D+ MP++ Sbjct: 9 KIQIVIIDDHQLFREGVKRILAMEPEFEVVADGEDGENAVELVEKYNPDVILMDINMPKV 68 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 NG++A R ++++YP +++ ++ + + T L+ GA ++ K Sbjct: 69 NGVEATRDLIQRYPDVKVLVLSIHDDESYVT-HVLKTGASGYLLK 112 >pir||T35758 probable response regulator - Streptomyces coelicolor >gi|5689906|emb|CAB52069.1| (AL109732) putative response regulator [Streptomyces coelicolor A3(2)] Length = 218 Score = 78.0 bits (189), Expect = 2e-13 Identities = 50/146 (34%), Positives = 85/146 (57%), Gaps = 18/146 (12%) Query: 2 PLTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDV 61 PLT RV++ +D A +R LK+++ + V GEA DG EA+E+ ++ RPD+V LDV Sbjct: 22 PLT---TRVVIAEDEALIRLDLKEMLEEEG-YSVVGEAGDGEEAVELAREHRPDLVILDV 77 Query: 62 EMPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMR 121 +MP+M+G+ A I ++ PV+M++A +Q + +A + GA+ ++ KP S Sbjct: 78 KMPKMDGISAAEKIAEESIAPVLMLTAFSQR--DLVERARDAGAMAYLVKPFS------- 128 Query: 122 ELKDEIIAKIKEAAKVPRRFLELRRI 147 K +++ I+ A RF EL+ + Sbjct: 129 --KSDVVPAIEMAVS---RFTELKAL 149 >sp|P52931|SP0A_BACCI STAGE 0 SPORULATION PROTEIN A >gi|497953|gb|AAA18874.1| (U09973) Spo0A [Bacillus circulans subsp. circulans] Length = 210 Score = 77.3 bits (187), Expect = 3e-13 Identities = 45/109 (41%), Positives = 72/109 (65%), Gaps = 4/109 (3%) Query: 6 RKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPR 65 +KI+V +VDD+ + +L++ I++ ++EV G A +G E +E+V++ PDV+ LD+ MP Sbjct: 2 KKIKVCIVDDNRELVGLLEEYIDAQDDMEVIGVAHNGQECLELVEQLEPDVMVLDIIMPH 61 Query: 66 MNGLDAL-RVIMKKYPVP-VIMISALTQEGAEATIKALEYGAIDFIPKP 112 ++GL L R+ P+P VIM++A QE + T KA+E GA FI KP Sbjct: 62 IDGLAVLERMRQMNKPMPNVIMLTAFGQE--DVTKKAVELGASYFILKP 108 >pir||S60871 phosphorylation-activated transcription factor Spo0A - Bacillus circulans (fragment) Length = 211 Score = 77.3 bits (187), Expect = 3e-13 Identities = 45/109 (41%), Positives = 72/109 (65%), Gaps = 4/109 (3%) Query: 6 RKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPR 65 +KI+V +VDD+ + +L++ I++ ++EV G A +G E +E+V++ PDV+ LD+ MP Sbjct: 2 KKIKVCIVDDNRELVGLLEEYIDAQDDMEVIGVAHNGQECLELVEQLEPDVMVLDIIMPH 61 Query: 66 MNGLDAL-RVIMKKYPVP-VIMISALTQEGAEATIKALEYGAIDFIPKP 112 ++GL L R+ P+P VIM++A QE + T KA+E GA FI KP Sbjct: 62 IDGLAVLERMRQMNKPMPNVIMLTAFGQE--DVTKKAVELGASYFILKP 108 >sp|P52934|SP0A_BACST STAGE 0 SPORULATION PROTEIN A >gi|2654216|emb|CAA05307.1| (AJ002297) Spo0A protein [Bacillus stearothermophilus] Length = 259 Score = 76.9 bits (186), Expect = 3e-13 Identities = 45/136 (33%), Positives = 84/136 (61%), Gaps = 6/136 (4%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+V + DD+ + +L + I+S P++EV G A +G + ++M+++ RPD++ LD+ MP ++ Sbjct: 3 IKVCIADDNRELVSLLDEYISSQPDMEVIGTAYNGQDCLQMLEEKRPDILLLDIIMPHLD 62 Query: 68 GLDALRVIMK--KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP--SSSISINMREL 123 GL L I ++ VIM++A QE + T KA+E GA FI KP +++ ++R++ Sbjct: 63 GLAVLERIRAGFEHQPNVIMLTAFGQE--DVTKKAVELGASYFILKPFDMENLAHHIRQV 120 Query: 124 KDEIIAKIKEAAKVPR 139 + +++AA P+ Sbjct: 121 YGKTTPVVRKAAPAPQ 136 >gb|AAD16452.1| (AF102271) response-regulator [Streptomyces coelicolor A3(2)] >gi|8568799|emb|CAB94548.1| (AL359152) chitinase two-component response regulator [Streptomyces coelicolor A3(2)] Length = 213 Score = 76.5 bits (185), Expect = 5e-13 Identities = 40/104 (38%), Positives = 66/104 (63%), Gaps = 2/104 (1%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVLV +D + +R L I+ S P++EV GEA DG +A+ + ++ RPD+V +DV+MPRM+ Sbjct: 2 IRVLVAEDQSAVRAGLVLILGSAPDIEVVGEAADGEQAVALARELRPDLVLMDVQMPRMD 61 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 G+ A R+++ + V++++ + E AL GA F+ K Sbjct: 62 GVSATRIVVGEQLADVLVLTTFDLD--EYVFGALRAGAAGFLLK 103 >dbj|BAB07347.1| (AP001519) two-component response regulator involved in degradative enzyme [Bacillus halodurans] Length = 236 Score = 75.7 bits (183), Expect = 8e-13 Identities = 33/105 (31%), Positives = 67/105 (63%), Gaps = 1/105 (0%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI+++++DD R+ +K I+ +PE EV + DG A+E+V+ PDV+ +D+ MP++ Sbjct: 9 KIQIVIIDDHQLFREGVKRILAMEPEFEVVADGEDGENAVELVETYNPDVILMDINMPKV 68 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 NG++A R ++++YP +++ ++ + + T L+ GA ++ K Sbjct: 69 NGVEATRDLIQRYPDVKVLVLSIHDDESYVT-HVLKTGASGYLLK 112 >sp|P55184|YXJL_BACSU HYPOTHETICAL TRANSCRIPTIONAL REGULATOR IN GALE-PEPT INTERGENIC REGION >gi|1429258|emb|CAA67717.1| (X99339) orf4; putative [Bacillus subtilis] Length = 218 Score = 75.3 bits (182), Expect = 1e-12 Identities = 42/130 (32%), Positives = 74/130 (56%), Gaps = 10/130 (7%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIRV + DD +R+ + +IN+ ++ V GEA DG + I + ++ +PDV+ +DV+MPR Sbjct: 5 KIRVALADDQPLVREAFRYVINAQTDMTVSGEAGDGHDIIALAKQTKPDVILMDVQMPRC 64 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +G++A + IM P I+I T + E + + GA+ ++ K + L +E Sbjct: 65 SGIEAAKDIMSALPNTKIVI-LTTFDTEEYVFEGIRAGAVGYLLKDT---------LPEE 114 Query: 127 IIAKIKEAAK 136 +I I+ AA+ Sbjct: 115 LIDAIRAAAR 124 >emb|CAA63989.1| (X94328) spo0a [Clostridium difficile] Length = 256 Score = 74.9 bits (181), Expect = 1e-12 Identities = 50/208 (24%), Positives = 111/208 (53%), Gaps = 9/208 (4%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI++++ DD+ +VLK+ ++++ ++++ G A+DGIEA+++V+K +PD++ LDV MP + Sbjct: 8 KIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGIEALDLVKKTQPDLLILDVIMPHL 67 Query: 67 NGLDALRVI----MKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRE 122 +GL + + + K P +I++SA+ Q+ + T A+ GA +I KP + + + Sbjct: 68 DGLGVIEKLNTMDIPKMP-KIIVLSAVGQD--KITQSAINLGADYYIVKPFDFV-VFINR 123 Query: 123 LKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARI-AVAIAASTGGPQSLL 181 +++ + ++ + PR E + R V+ ++ + I + + A G L Sbjct: 124 IRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIETEITNIIHEIGVPAHIKGYLYLR 183 Query: 182 KIFPKFPENLKAGILLVQHMPPGFTRSF 209 + +N++ + + + P + F Sbjct: 184 EAMKMVIDNVELLGAVTKELYPSIAKKF 211 >sp|P52938|SP0A_CLODI STAGE 0 SPORULATION PROTEIN A HOMOLOG >gi|1174099|gb|AAB05561.1| (U43514) phosphorylation-activated transcription factor spo0a [Clostridium difficile] Length = 274 Score = 74.9 bits (181), Expect = 1e-12 Identities = 50/208 (24%), Positives = 111/208 (53%), Gaps = 9/208 (4%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI++++ DD+ +VLK+ ++++ ++++ G A+DGIEA+++V+K +PD++ LDV MP + Sbjct: 8 KIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGIEALDLVKKTQPDLLILDVIMPHL 67 Query: 67 NGLDALRVI----MKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRE 122 +GL + + + K P +I++SA+ Q+ + T A+ GA +I KP + + + Sbjct: 68 DGLGVIEKLNTMDIPKMP-KIIVLSAVGQD--KITQSAINLGADYYIVKPFDFV-VFINR 123 Query: 123 LKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARI-AVAIAASTGGPQSLL 181 +++ + ++ + PR E + R V+ ++ + I + + A G L Sbjct: 124 IRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIETEITNIIHEIGVPAHIKGYLYLR 183 Query: 182 KIFPKFPENLKAGILLVQHMPPGFTRSF 209 + +N++ + + + P + F Sbjct: 184 EAMKMVIDNVELLGAVTKELYPSIAKKF 211 >pir||H70558 hypothetical protein Rv1626 - Mycobacterium tuberculosis (strain H37RV) >gi|2113910|emb|CAB08879.1| (Z95554) hypothetical protein Rv1626 [Mycobacterium tuberculosis] Length = 205 Score = 74.9 bits (181), Expect = 1e-12 Identities = 51/154 (33%), Positives = 89/154 (57%), Gaps = 15/154 (9%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 RVL+ +D A +R L +++ + E+ GEA DG EA+E+ + +PD+V +DV+MPR +G Sbjct: 15 RVLIAEDEALIRMDLAEMLREEG-YEIVGEAGDGQEAVELAELHKPDLVIMDVKMPRRDG 73 Query: 69 LDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSS------SISINMRE 122 +DA I K P+++++A +Q + +A + GA+ ++ KP S +I + + Sbjct: 74 IDAASEIASKRIAPIVVLTAFSQR--DLVERARDAGAMAYLVKPFSISDLIPAIELAVSR 131 Query: 123 LKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAK 156 + EI A E A + R LE R++ V++AK Sbjct: 132 FR-EITALEGEVATLSER-LETRKL----VERAK 159 >pir||T34653 probable response regulator - Streptomyces coelicolor >gi|4007689|emb|CAA22374.1| (AL034446) putative response regulator [Streptomyces coelicolor A3(2)] Length = 219 Score = 74.9 bits (181), Expect = 1e-12 Identities = 39/107 (36%), Positives = 66/107 (61%), Gaps = 5/107 (4%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRV++ DD A +R L I+++ P+LEV GE DG +++ ++ RPDVV +D+ MP ++ Sbjct: 5 IRVVICDDQALIRTGLATIVDAQPDLEVVGECGDGQTGVDLARELRPDVVVMDIRMPVLD 64 Query: 68 GLDALRVIM---KKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 GL+A R++ +PV V++++ + E +AL GA F+ K Sbjct: 65 GLEATRLLAGAGVAHPVKVLVVTTFNLD--EYVYEALRAGASGFLLK 109 >emb|CAB59507.1| (AL132648) putative two-component response regulator [Streptomyces coelicolor A3(2)] Length = 223 Score = 74.9 bits (181), Expect = 1e-12 Identities = 40/107 (37%), Positives = 68/107 (63%), Gaps = 5/107 (4%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVL+VDD +R + I+ ++ +L V GEA DG++A++ V+ +PDVV +D+ MPRM+ Sbjct: 3 IRVLLVDDQPLLRTGFRMILEAEQDLAVVGEAGDGLQALDQVRALQPDVVLMDIRMPRMD 62 Query: 68 GLDALRVIM---KKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 G++A R I + P V++++ + E ++AL+ GA F+ K Sbjct: 63 GVEATRQITGPERDGPAKVLVLTTFDLD--EYVVEALKAGASGFLLK 107 >pir||T36318 two-component response regulator - Streptomyces coelicolor >gi|1293660|gb|AAB08053.1| (U51332) AbsA2 [Streptomyces coelicolor] >gi|4490635|emb|CAB38597.1| (AL035654) two component response regulator [Streptomyces coelicolor A3(2)] Length = 222 Score = 74.5 bits (180), Expect = 2e-12 Identities = 42/107 (39%), Positives = 63/107 (58%), Gaps = 3/107 (2%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVL+ DD +R ++ I+ ++P +EV EA DG EA+E+ +K RPDV LD+ MP M+ Sbjct: 2 IRVLLADDETIIRAGVRSILTTEPGIEVVAEASDGREAVELARKHRPDVALLDIRMPEMD 61 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATI-KALEYGAIDFIPKPS 113 GL A + P +++ LT G + I +AL+ G F+ K S Sbjct: 62 GLTAAGEMRTTNPDTAVVV--LTTFGEDRYIERALDQGVAGFLLKAS 106 >pir||T36530 probable two-component system regulator - Streptomyces coelicolor >gi|4753864|emb|CAB42027.1| (AL049754) putative two-component system regulator [Streptomyces coelicolor A3(2)] Length = 228 Score = 74.5 bits (180), Expect = 2e-12 Identities = 39/112 (34%), Positives = 68/112 (59%), Gaps = 3/112 (2%) Query: 5 GRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMP 64 GR IRVL+ DD +R+ ++N+ P++EV G+A DG++A+ V + P+VV +D+ MP Sbjct: 7 GRVIRVLIADDQQMVRQGFTVLLNTQPDIEVIGQAVDGLDAVAKVAELAPEVVLMDIRMP 66 Query: 65 RMNGLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSSS 115 + G++A R I P + V++++ + E +AL GA F+ K +S+ Sbjct: 67 ELGGIEATRRITTATPEIRVLVLTTFDLD--EYVYEALRAGASGFLLKDASA 116 >pir||A70080 two-component response regulator [YxjM] homolog yxjL - Bacillus subtilis >gi|1783236|dbj|BAA11713.1| (D83026) homologous to many transcriptional activator proteins; hypothetical [Bacillus subtilis] >gi|2636426|emb|CAB15917.1| (Z99123) similar to two-component response regulator [YxjM] [Bacillus subtilis] Length = 218 Score = 74.1 bits (179), Expect = 2e-12 Identities = 42/130 (32%), Positives = 74/130 (56%), Gaps = 10/130 (7%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIRV + DD +R+ + +IN+ ++ V GEA DG + I + ++ +PDV+ +DV+MPR Sbjct: 5 KIRVALADDQPLVREGFRYVINAQTDMTVSGEAGDGHDIIALAKQTKPDVILMDVQMPRC 64 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +G++A + IM P I+I T + E + + GA+ ++ K + L +E Sbjct: 65 SGIEAAKDIMSALPNTKIVI-LTTFDTEEYVFEGIRAGAVGYLLKDT---------LPEE 114 Query: 127 IIAKIKEAAK 136 +I I+ AA+ Sbjct: 115 LIDAIRAAAR 124 >pir||G69780 two-component response regulator [YdfH] homolog ydfI - Bacillus subtilis >gi|1881349|dbj|BAA19376.1| (AB001488) PROBABLE REGULATORY COMPONENT OF SENSORY TRANSDUCTION SYSTEM. [Bacillus subtilis] >gi|2632842|emb|CAB12349.1| (Z99106) similar to two-component response regulator [YdfH] [Bacillus subtilis] Length = 213 Score = 74.1 bits (179), Expect = 2e-12 Identities = 34/107 (31%), Positives = 68/107 (62%), Gaps = 2/107 (1%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 +VL+VDD +R+ LK +I ++ + + GEA +G A+ + + PD++ +D+ MP M+G Sbjct: 3 KVLIVDDHLVVREGLKLLIETNDQYTIIGEAENGKVAVRLADELEPDIILMDLYMPEMSG 62 Query: 69 LDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSS 115 L+A++ I +K+ P+I+++ ++ I+ +E GA ++ K +SS Sbjct: 63 LEAIKQIKEKHDTPIIILTTYNED--HLMIEGIELGAKGYLLKDTSS 107 >pir||T35281 probable two component response regulator - Streptomyces coelicolor >gi|5578847|emb|CAB51249.1| (AL096872) putative two component response regulator [Streptomyces coelicolor A3(2)] Length = 227 Score = 74.1 bits (179), Expect = 2e-12 Identities = 44/129 (34%), Positives = 72/129 (55%), Gaps = 10/129 (7%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVL+ DD A +R + +++S+P++EV GEA DG EA + + R DVV +D+ MP + Sbjct: 3 IRVLLADDQALLRSAFRVLVDSEPDMEVVGEASDGAEAARLAAEQRADVVLMDIRMPGTD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQ-EGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 GL A R+I + + + LT E + +++L GA F+ K S +E Sbjct: 63 GLAATRMISADPSLAHVRVVILTTFEVDDYVVQSLRAGASGFLGKGSE---------PEE 113 Query: 127 IIAKIKEAA 135 +++ I+ AA Sbjct: 114 LLSAIRVAA 122 >pir||H75381 DNA-binding response regulator - Deinococcus radiodurans (strain R1) >gi|6459320|gb|AAF11120.1|AE001999_4 (AE001999) DNA-binding response regulator [Deinococcus radiodurans] Length = 225 Score = 74.1 bits (179), Expect = 2e-12 Identities = 55/168 (32%), Positives = 92/168 (54%), Gaps = 13/168 (7%) Query: 3 LTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVE 62 +T +RVL+VDD A +R+ L+ + D +EV GEA +G EA++ ++ RP+VV +D+ Sbjct: 9 MTTPTVRVLLVDDHAVVRQGLRLFLGLDEGIEVVGEAANGEEALQEAERLRPEVVVMDLM 68 Query: 63 MPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIK-ALEYGAIDFIPKPSSSISINMR 121 MP M+G+ A R + ++ P ++ ALT E + A+E GAI ++ K +SS Sbjct: 69 MPVMDGITATRELRRRLPDTEVI--ALTSTLEENKVNGAIEAGAISYMLKDASS------ 120 Query: 122 ELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSV-PARIAV 168 D + I AA+ R RL+R ++ +++ S+ P AV Sbjct: 121 ---DTLADAIHAAARGEVRLHPEAARRLVRDFRSPEMRESLTPKETAV 165 >emb|CAB54571.1| (AJ006393) response regulator [Streptococcus pneumoniae] Length = 210 Score = 73.4 bits (177), Expect = 4e-12 Identities = 38/108 (35%), Positives = 65/108 (60%), Gaps = 1/108 (0%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +++L+VDD +R LK + ++EV GEA +G + I++ + RPDV+ +D+ MP MN Sbjct: 1 MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALELRPDVIVMDIVMPEMN 60 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSS 115 G+DA I+K++P I+I + E + L+ GA ++ K SS+ Sbjct: 61 GIDATLAILKEWPEAKILIVTSYLDN-EKIMPVLDAGAKGYMLKTSSA 107 >emb|CAB87987.1| (AJ288948) Spo0A protein [Clostridium cellulolyticum] Length = 292 Score = 73.4 bits (177), Expect = 4e-12 Identities = 45/129 (34%), Positives = 78/129 (59%), Gaps = 9/129 (6%) Query: 3 LTGRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVE 62 L+ +KI VL+ DD+ +L + +++ ++EV G ARDG EA++++ + PD+ LD+ Sbjct: 12 LSSKKIEVLIADDNREFGDILCEYLSNQEDIEVVGLARDGFEAVDLILQNTPDIAILDII 71 Query: 63 MPRMNGLDALRVI----MKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP--SSSI 116 MP ++GL L I ++K P+ I++SA+ Q+ + T +AL GA +I KP + Sbjct: 72 MPHLDGLGVLEKIASSNLEKKPL-FIVLSAVGQD--KITQRALSLGAEYYIVKPFDMDVL 128 Query: 117 SINMRELKD 125 +R+LKD Sbjct: 129 VSRIRQLKD 137 >pir||T36528 probable two-component system regulator - Streptomyces coelicolor >gi|4753862|emb|CAB42025.1| (AL049754) putative two-component system regulator [Streptomyces coelicolor A3(2)] Length = 221 Score = 73.4 bits (177), Expect = 4e-12 Identities = 42/120 (35%), Positives = 70/120 (58%), Gaps = 6/120 (5%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVL+ DD +R+ ++N+ P++EV GEA +G EA+ V++ PDVV +D+ MP +N Sbjct: 3 IRVLIADDQMMVREGFSVLLNAMPDIEVAGEAVNGREAVAKVRELSPDVVLMDIRMPELN 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G++A R I+ +++ T + E +AL GA F+ K +S+ R+L D + Sbjct: 63 GIEATREIVAADSTSKVLV-LTTFDLDEYVYQALRAGASGFLLKDASA-----RQLADGV 116 >pir||T30314 chpB protein - Pseudomonas aeruginosa >gi|11351447|pir||F83594 probable methylesterase PA0414 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|3241970|gb|AAC23932.1| (U79580) ChpB [Pseudomonas aeruginosa] >gi|9946269|gb|AAG03803.1|AE004478_8 (AE004478) probable methylesterase [Pseudomonas aeruginosa] Length = 343 Score = 73.0 bits (176), Expect = 5e-12 Identities = 79/354 (22%), Positives = 141/354 (39%), Gaps = 44/354 (12%) Query: 9 RVLVVDDSAFMRKVLKD---------IINSDPELEVCGEARDGIEAIEMVQKCRPDVVTL 59 RV V+ D++ R VL+ ++N+DP AR A+E C PD+ + Sbjct: 8 RVAVIADTSLQRHVLQQALLGHGYEVVLNADP-------ARVDDAALE----CAPDLWLV 56 Query: 60 DVEMPRMNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAID----FIPKPSSS 115 D + + + L ++++ PV+ E E I I PS Sbjct: 57 D--LTQQDDSPLLDSLLEQDRAPVLFGEGHAPERHTEHYPRWERRLIGKLKRLIGDPSDG 114 Query: 116 ISINMRELKDEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAIAASTG 175 + ++ L DE + PR + + A ++ AR +AAS G Sbjct: 115 VGDSLGALLDE--------ERPPR-------LEIPGDLAAMPLQAGEAAREVWLLAASLG 159 Query: 176 GPQSLLKIFPKFPENLKAGILLVQHMPPGFTRSFAKRLDSVSKIDVKEAEEGDVVEEGKA 235 GP ++ P L G L QH+ F ++ + + S+ VK +G+ + G+ Sbjct: 160 GPAAVKAFLDALPGGLPIGFLYAQHIDASFEQNLPQAVGRHSQWHVKNVRDGEALRCGEV 219 Query: 236 YVAPGDYHMEVTLRGGKPVITLNKKPKMHGVRPAADPMMITAAQVFGRRTVGVVMTGMGR 295 V P + + G + +++ P+ D MM+ AQ FG +V +GMG Sbjct: 220 MVVPVLHELGFHHDGR---LKCSQRSWPEPYTPSIDQMMLNLAQQFGPDCGVIVFSGMGS 276 Query: 296 DGAQGIVEIKKRGGITIAQDEKTSIIFGMPKAAIETGMVDYVVPLEKIPETVVR 349 DG+ ++++GG Q + + MP + E G + ++ E +V+ Sbjct: 277 DGSAAAAYVRRQGGEVWTQRADSCVCSSMPDSLREAGYSSFNASPRELAEALVK 330 >pir||T35166 probable two-component regulator - Streptomyces coelicolor >gi|3334798|emb|CAA19946.1| (AL031107) putative two-component regulator [Streptomyces coelicolor A3(2)] Length = 222 Score = 73.0 bits (176), Expect = 5e-12 Identities = 39/104 (37%), Positives = 60/104 (57%), Gaps = 2/104 (1%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 +RV+V DD +R I+ + P++EV EA DG EA+ V++ PDV+ LD+ MP M+ Sbjct: 3 VRVVVADDQELVRSGFSMILEAQPDIEVVAEAGDGAEAVAAVERYAPDVLLLDIRMPVMD 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 GLDA R + + V+M++ + E +AL GA F+ K Sbjct: 63 GLDAARRVCARSACKVVMLTTFDLD--EYVYEALYAGASGFLLK 104 >pir||T36862 probable two-component response regulator - Streptomyces coelicolor >gi|5123537|emb|CAB45293.1| (AL079332) putative two component response regulator [Streptomyces coelicolor A3(2)] Length = 210 Score = 73.0 bits (176), Expect = 5e-12 Identities = 37/105 (35%), Positives = 66/105 (62%), Gaps = 3/105 (2%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+VL+VDD +R+ L+ + ++EV GEA DG E ++ Q+ +PDV+ +DV+MP M+ Sbjct: 2 IKVLLVDDHQVVRRGLRTFLEVQDDIEVVGEAADGAEGVDRAQELKPDVILMDVKMPGMD 61 Query: 68 GLDALRVIMK-KYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 G+DALR + + V+++++ T++ + AL GA ++ K Sbjct: 62 GVDALRRLRELDNHARVLVVTSFTEQ--RTVVPALRAGAAGYVYK 104 >sp|P33356|YEHT_ECOLI HYPOTHETICAL 27.9 KD PROTEIN IN MOLR-BGLX INTERGENIC REGION >gi|7449507|pir||D64980 yehT protein - Escherichia coli (strain K-12) >gi|405856|gb|AAA60488.1| (U00007) yehT [Escherichia coli] >gi|2367130|gb|AAC75186.1| (AE000301) orf, hypothetical protein [Escherichia coli K12] >gi|744225|prf||2014253V yehT gene [Escherichia coli] Length = 244 Score = 72.6 bits (175), Expect = 7e-12 Identities = 36/105 (34%), Positives = 63/105 (59%), Gaps = 4/105 (3%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+VL+VDD R+ L+ + ++E+ GE + +E I V K RPDV+ LD++MPR++ Sbjct: 7 IKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVHKLRPDVLFLDIQMPRIS 66 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 GL+ + ++ ++ ++ ++A E IKA E A D++ KP Sbjct: 67 GLEMVGMLDPEHRPYIVFLTAFD----EYAIKAFEEHAFDYLLKP 107 >gb|AAC33471.1| (AF074950) CheY [Treponema denticola] Length = 146 Score = 72.6 bits (175), Expect = 7e-12 Identities = 49/111 (44%), Positives = 67/111 (60%), Gaps = 6/111 (5%) Query: 5 GRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRP--DVVTLDVE 62 G ++LVVDDS F+ K + I+NS+ EV A DG E +E ++ P D+VT+D+ Sbjct: 22 GTPYKILVVDDSIFVTKQIGQILNSEG-YEVVATAVDGFEGVEKYKELCPNVDLVTMDIT 80 Query: 63 MPRMNGLDALRVIMK-KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 MP+M+GL AL IM V+MISAL +E E KAL GA ++I KP Sbjct: 81 MPKMDGLTALEQIMAFDKNAKVVMISALGKE--ELVKKALLLGAKNYIVKP 129 >pdb|1DZ3|A Chain A, Domain-Swapping In The Sporulation Response Regulator Spo0a Length = 130 Score = 72.6 bits (175), Expect = 7e-12 Identities = 41/107 (38%), Positives = 68/107 (63%), Gaps = 4/107 (3%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+V + DD+ + +L + I+S P++EV G A +G + ++M+++ RPD++ LD+ MP ++ Sbjct: 3 IKVCIADDNRELVSLLDEYISSQPDMEVIGTAYNGQDCLQMLEEKRPDILLLDIIMPHLD 62 Query: 68 GLDALRVIMK--KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 GL L I ++ VIM++A QE + T KA+E GA FI KP Sbjct: 63 GLAVLERIRAGFEHQPNVIMLTAFGQE--DVTKKAVELGASYFILKP 107 >emb|CAB90919.1| (AL355774) putative DNA-binding response regulator [Streptomyces coelicolor A3(2)] Length = 252 Score = 72.6 bits (175), Expect = 7e-12 Identities = 43/128 (33%), Positives = 71/128 (54%), Gaps = 10/128 (7%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVLVVDD A R+ L+ ++ ++ +++V GEA DG EA+E PD+V +DV MP+ Sbjct: 36 IRVLVVDDHALFRRGLEIVLAAEEDIQVVGEAGDGAEAVEKAADLLPDIVLMDVRMPKRG 95 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G++A I + P I++ ++ E A+ A++ GA ++ K S+ DE+ Sbjct: 96 GIEACTSIKEVAPSAKIIMLTISDEEAD-LYDAIKAGATGYLLKEIST---------DEV 145 Query: 128 IAKIKEAA 135 I+ A Sbjct: 146 ATAIRAVA 153 >emb|CAB95289.1| (AL359779) putative two component system response regulator [Streptomyces coelicolor A3(2)] Length = 221 Score = 72.6 bits (175), Expect = 7e-12 Identities = 39/105 (37%), Positives = 63/105 (59%), Gaps = 1/105 (0%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVL+ DD + +R + ++++ P++EV EA DG EA+ +++ RPDVV +D+ MP ++ Sbjct: 2 IRVLLADDQSLVRAGFRALLDAQPDIEVVAEAADGEEAVRSIRELRPDVVLMDIRMPLLD 61 Query: 68 GLDALRVIMKKYPVPVIMISALTQ-EGAEATIKALEYGAIDFIPK 111 GL A R I +P + + LT E E +A+ GA F+ K Sbjct: 62 GLAATRRITGDPGLPDVRVVMLTTFELDEYVFEAIRSGASGFLVK 106 >sp|P37478|YYCF_BACSU HYPOTHETICAL 27.2 KD SENSORY TRANSDUCTION PROTEIN IN ROCR-PURA INTERGENIC REGION >gi|2126981|pir||S65967 two-component response regulator yycF - Bacillus subtilis >gi|467327|dbj|BAA05173.1| (D26185) unknown [Bacillus subtilis] >gi|1064814|dbj|BAA11300.1| (D78193) homologous to sp:PHOP_BACSUB [Bacillus subtilis] >gi|2636588|emb|CAB16078.1| (Z99124) similar to two-component response regulator [YycG] [Bacillus subtilis] Length = 235 Score = 72.2 bits (174), Expect = 9e-12 Identities = 42/124 (33%), Positives = 73/124 (58%), Gaps = 9/124 (7%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 ++LVVDD + +L+ + + C A DG EA+EMV++ +PD++ LD+ +P +G Sbjct: 4 KILVVDDEKPIADILEFNLRKEGYEVHC--AHDGNEAVEMVEELQPDLILLDIMLPNKDG 61 Query: 69 LDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEII 128 ++ R + KKY +P+IM++A E + + LE GA D++ KP S+ REL + Sbjct: 62 VEVCREVRKKYDMPIIMLTAKDSEIDK--VIGLEIGADDYVTKPFST-----RELLARVK 114 Query: 129 AKIK 132 A ++ Sbjct: 115 ANLR 118 >pir||S58645 response regulator cheY [validated] - Halobacterium salinarum >gi|994802|emb|CAA60161.1| (X86407) cheY [Halobacterium salinarum] >gi|10580529|gb|AAG19395.1| (AE005033) chemotaxis protein; CheY [Halobacterium sp. NRC-1] Length = 120 Score = 72.2 bits (174), Expect = 9e-12 Identities = 45/122 (36%), Positives = 75/122 (60%), Gaps = 6/122 (4%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 +VL+VDDS FMR +L++I+ + E E+ EA +G+EA+EM ++ PD+V +D+ MP +G Sbjct: 4 QVLLVDDSEFMRNLLREIL--EEEFEIADEAENGVEAVEMYKEYDPDLVMMDIVMPIRDG 61 Query: 69 LDALRVIMK-KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 ++A I + +IM +++ QE E KA++ GA +I KP S+ M + D + Sbjct: 62 IEATSEIKEFDAGAHIIMCTSIGQE--EKMKKAVKAGADGYITKPFQKPSV-MDAISDVL 118 Query: 128 IA 129 A Sbjct: 119 TA 120 >pir||I40540 vsrD protein - Pseudomonas solanacearum >gi|603069|gb|AAA66240.1| (U18135) VsrD [Ralstonia solanacearum] Length = 210 Score = 71.8 bits (173), Expect = 1e-11 Identities = 36/108 (33%), Positives = 66/108 (60%), Gaps = 3/108 (2%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVL+ DD +R L+ ++ + ++EV GEA G E +E ++ DVV LD+ MP N Sbjct: 2 IRVLIADDHEIVRAGLRQFLSEERDIEVAGEAASGEEVMEQLRTEAFDVVVLDISMPDRN 61 Query: 68 GLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSS 114 G+D L+++ +++P +PV+++S ++ + I + GA ++ K S+ Sbjct: 62 GIDTLKLVRQRHPDLPVLILSTFPED--QYAINLIRAGASGYLTKESA 107 >dbj|BAA88407.1| (AB016841) chiR [Streptomyces thermoviolaceus] Length = 213 Score = 71.8 bits (173), Expect = 1e-11 Identities = 44/129 (34%), Positives = 72/129 (55%), Gaps = 11/129 (8%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IRVLV +D + +R L I+ S P +EV GEA DG A+++ + RPDVV +DV MPR++ Sbjct: 2 IRVLVAEDQSAVRAGLVLILRSAPGIEVAGEAEDGQRAVDLARDLRPDVVLMDVRMPRLD 61 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+ A R I+ + V++++ T + AL GA F+ K + + D++ Sbjct: 62 GVSATRQIVAEQLADVLVLT--TFDFDAYVFGALRAGAAGFLLKSAEA---------DDL 110 Query: 128 IAKIKEAAK 136 I ++ A+ Sbjct: 111 IDAVRTVAR 119 >dbj|BAB05816.1| (AP001514) two-component response regulator [Bacillus halodurans] Length = 210 Score = 71.8 bits (173), Expect = 1e-11 Identities = 46/163 (28%), Positives = 95/163 (58%), Gaps = 9/163 (5%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+VL+ +D +R+ LK +I +D ++ V GEA +G EAI + + R DV LD+ MP M+ Sbjct: 2 IKVLLAEDQVMVRQGLKVMIETDEDIFVTGEATNGKEAIRLCESQRFDVAVLDIRMPEMD 61 Query: 68 GLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 G++A +V+ ++P + ++M++ T + + ++AL+ G ++ K + S+ +R +K Sbjct: 62 GIEAAKVLRSRFPEMKILMLT--TFDDHQYVMEALKLGVSGYMLKDGDTESL-IRSIKSA 118 Query: 127 IIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSV-PARIAV 168 + + +V + + + LL+ ++A + P++ P IA+ Sbjct: 119 LNGGLSLEDQVAAKVMPV----LLQHEEAIPIDPTLTPREIAI 157 >gb|AAB07804.1| (U01971) MtrA [Mycobacterium tuberculosis] Length = 225 Score = 71.4 bits (172), Expect = 2e-11 Identities = 45/142 (31%), Positives = 76/142 (52%), Gaps = 11/142 (7%) Query: 9 RVLVVDDSAFMRKVLKDIINSDP-ELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 R+LVVDD A + ++L ++ + + V G DG +A+ V++ RPD+V LD+ +P MN Sbjct: 4 RILVVDDDASLAEMLTIVLRGEGFDTAVIG---DGTQALTAVRELRPDLVLLDLMLPGMN 60 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+D RV+ VP++M++A T + LE GA D+I KP +EL + Sbjct: 61 GIDVCRVLRADSGVPIVMLTAKTD--TVDVVLGLESGADDYIMKP-----FKPKELVARV 113 Query: 128 IAKIKEAAKVPRRFLELRRIRL 149 A+++ P L + + + Sbjct: 114 RARLRRNDDEPAEMLSIADVEI 135 >pir||H70592 probable mtrA protein - Mycobacterium tuberculosis (strain H37RV) >gi|2072713|emb|CAB08347.1| (Z95121) mtrA [Mycobacterium tuberculosis] Length = 228 Score = 71.4 bits (172), Expect = 2e-11 Identities = 45/142 (31%), Positives = 76/142 (52%), Gaps = 11/142 (7%) Query: 9 RVLVVDDSAFMRKVLKDIINSDP-ELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 R+LVVDD A + ++L ++ + + V G DG +A+ V++ RPD+V LD+ +P MN Sbjct: 7 RILVVDDDASLAEMLTIVLRGEGFDTAVIG---DGTQALTAVRELRPDLVLLDLMLPGMN 63 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G+D RV+ VP++M++A T + LE GA D+I KP +EL + Sbjct: 64 GIDVCRVLRADSGVPIVMLTAKTD--TVDVVLGLESGADDYIMKP-----FKPKELVARV 116 Query: 128 IAKIKEAAKVPRRFLELRRIRL 149 A+++ P L + + + Sbjct: 117 RARLRRNDDEPAEMLSIADVEI 138 >pir||T34675 probable response regulator - Streptomyces coelicolor >gi|4007711|emb|CAA22396.1| (AL034446) putative response regulator [Streptomyces coelicolor A3(2)] Length = 260 Score = 71.0 bits (171), Expect = 2e-11 Identities = 38/106 (35%), Positives = 65/106 (60%), Gaps = 3/106 (2%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KIR+L+ DD A +R+ ++ I++ +P+LEV EA DG EAI+M + D+ LD+ MPR+ Sbjct: 47 KIRILLADDHALVRRGVRLILDREPDLEVVAEAGDGAEAIDMARAHEADLAVLDIAMPRL 106 Query: 67 NGLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPK 111 GL A R + P + ++M++ + + +AL+ GA ++ K Sbjct: 107 TGLQAARELAALKPGLRILMLT--MHDNEQYLFQALKSGACGYVLK 150 >sp|P52932|SP0A_BACME STAGE 0 SPORULATION PROTEIN A >gi|497955|gb|AAA18875.1| (U09974) Spo0A [Bacillus megaterium] Length = 212 Score = 70.6 bits (170), Expect = 3e-11 Identities = 44/110 (40%), Positives = 69/110 (62%), Gaps = 5/110 (4%) Query: 6 RKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPR 65 +KI+V +VDD+ + +L+D I+ ++EV G A +G E + +++ PDV+ LD+ MP Sbjct: 2 KKIKVCLVDDNRELIGLLEDYISEQEDMEVIGVAYNGQECLSILKDKNPDVLVLDIIMPH 61 Query: 66 MNGLDALRVI--MKKYPVP-VIMISALTQEGAEATIKALEYGAIDFIPKP 112 ++GL L + MKK P VIM++A QE + T KA++ GA FI KP Sbjct: 62 LDGLAVLGQLKEMKKENYPNVIMLTAFGQE--DVTKKAVDLGAAYFILKP 109 >pir||F70645 hypothetical protein Rv3133c - Mycobacterium tuberculosis (strain H37RV) >gi|1781234|emb|CAB06281.1| (Z83867) hypothetical protein Rv3133c [Mycobacterium tuberculosis] >gi|4335782|gb|AAD17452.1| (U22037) DevR [Mycobacterium tuberculosis] Length = 217 Score = 70.6 bits (170), Expect = 3e-11 Identities = 40/119 (33%), Positives = 70/119 (58%), Gaps = 4/119 (3%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 ++V +VDD +R+ L D++ +DPEL+V GEA EA+ V RPDV LDV +P N Sbjct: 2 VKVFLVDDHEVVRRGLVDLLGADPELDVVGEAGSVAEAMARVPAARPDVAVLDVRLPDGN 61 Query: 68 GLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKD 125 G++ R ++ + P + +++++ T + EA + A+ GA ++ K + + R +KD Sbjct: 62 GIELCRDLLSRMPDLRCLILTSYTSD--EAMLDAILAGASGYVVKDIKGMEL-ARAVKD 117 >pir||S60872 phosphorylation-activated transcription factor Spo0A - Bacillus megaterium (fragment) Length = 213 Score = 70.6 bits (170), Expect = 3e-11 Identities = 44/110 (40%), Positives = 69/110 (62%), Gaps = 5/110 (4%) Query: 6 RKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPR 65 +KI+V +VDD+ + +L+D I+ ++EV G A +G E + +++ PDV+ LD+ MP Sbjct: 2 KKIKVCLVDDNRELIGLLEDYISEQEDMEVIGVAYNGQECLSILKDKNPDVLVLDIIMPH 61 Query: 66 MNGLDALRVI--MKKYPVP-VIMISALTQEGAEATIKALEYGAIDFIPKP 112 ++GL L + MKK P VIM++A QE + T KA++ GA FI KP Sbjct: 62 LDGLAVLGQLKEMKKENYPNVIMLTAFGQE--DVTKKAVDLGAAYFILKP 109 >pir||F75593 DNA-binding response regulator - Deinococcus radiodurans (strain R1) >gi|6460582|gb|AAF12288.1|AE001862_114 (AE001862) DNA-binding response regulator [Deinococcus radiodurans] Length = 211 Score = 70.6 bits (170), Expect = 3e-11 Identities = 51/179 (28%), Positives = 93/179 (51%), Gaps = 11/179 (6%) Query: 1 MPLTGR---KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVV 57 +P TG IRVL+ +D + L +++ + +L+V G A DG EA+ V RPDV+ Sbjct: 3 LPETGAGSSPIRVLLAEDQTLVLGALAALLSLEDDLDVVGTAADGAEALMQVHTLRPDVL 62 Query: 58 TLDVEMPRMNGLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPK--PSS 114 D+EMPR++GLD + ++ P V++++ T+ G +AL+ GA ++ K PS Sbjct: 63 VTDIEMPRLSGLDLAERVRQEAPQTRVVIVTTFTRAG--YLRRALDAGARGYLLKDAPSH 120 Query: 115 SISINMRELK---DEIIAKIKEAAKVPRRFLELRRIRLLRVQKAKKVKPSVPARIAVAI 170 ++ +R + I A + E + L R I++LR + + ++ A + + + Sbjct: 121 ELADAIRRVHAGGRAIDAGLAEEIWDAQSPLTEREIQVLRAAETGRSTAAIAAELGIGV 179 >pir||C81104 transcription regulator, LuxR family NMB1250 [imported] - Neisseria meningitidis (group B strain MD58, group A strain Z2491) >gi|7226490|gb|AAF41630.1| (AE002473) transcriptional regulator, LuxR family [Neisseria meningitidis MC58] >gi|7380078|emb|CAB84659.1| (AL162755) putative two-component system response regulator [Neisseria meningitidis Z2491] Length = 218 Score = 70.6 bits (170), Expect = 3e-11 Identities = 39/129 (30%), Positives = 75/129 (57%), Gaps = 10/129 (7%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+++++DD R +K +++ EV GEA DG+ I+M+ + +PDVV LD++MP MN Sbjct: 3 IKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMISRLQPDVVLLDLDMPGMN 62 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 G +AL I+ P +++ ++++ + T + + GA ++ K +IN D + Sbjct: 63 GREALSQIISINPQQAVIMLTVSEDSDDLT-ECMRIGARGYLLK-----NIN----ADFL 112 Query: 128 IAKIKEAAK 136 + I++AA+ Sbjct: 113 LESIRKAAE 121 >pir||H82218 transcription regulator LuxR family VC1277 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9655763|gb|AAF94436.1| (AE004207) transcriptional regulator, LuxR family [Vibrio cholerae] Length = 216 Score = 70.6 bits (170), Expect = 3e-11 Identities = 36/113 (31%), Positives = 70/113 (61%), Gaps = 1/113 (0%) Query: 6 RKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPR 65 + IRV++VDD + + +PE+EV A +G+EA+E+V+ +PDVV +DV MP Sbjct: 7 KPIRVVMVDDHQVVLDGFIARLEQEPEIEVVATASNGLEALELVKLHQPDVVLMDVSMPI 66 Query: 66 MNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISI 118 MNG++A R+I ++ P +++ + + E ++ ++ GA+ ++ K S++ + Sbjct: 67 MNGIEATRLIKEEVPHTKVLMLTM-HDNREYIMQVMQAGAMGYMLKEISALKM 118 >dbj|BAB05842.1| (AP001514) two-component response regulator [Bacillus halodurans] Length = 223 Score = 70.6 bits (170), Expect = 3e-11 Identities = 38/105 (36%), Positives = 66/105 (62%), Gaps = 3/105 (2%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I VL+ DD MR+ LK II+ + ++ V G A++G E ++ + P V+ +D++MP MN Sbjct: 4 INVLIADDQTLMREGLKTIIDLEDDMNVTGLAKNGKEVLDQIPCLLPHVILMDIQMPIMN 63 Query: 68 GLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPK 111 G++ L+V+ + YP + V+M++ T E + I++L GA F+ K Sbjct: 64 GIECLKVVKRIYPHINVLMLT--TFEDDDYIIESLVGGANGFLLK 106 >dbj|BAB05932.1| (AP001514) two-component response regulator [Bacillus halodurans] Length = 217 Score = 70.6 bits (170), Expect = 3e-11 Identities = 39/99 (39%), Positives = 64/99 (64%), Gaps = 3/99 (3%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IR+ VVDD +RK L + + + + GEA DG A+ +V+K +PD+V +DV MP +N Sbjct: 3 IRIAVVDDHHVVRKGLIYFLETYHDFTIVGEAEDGEAALTLVEKVQPDIVLMDVIMPGIN 62 Query: 68 GLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGA 105 G++A + I +K+P V V+++S+ + E+ I AL+ GA Sbjct: 63 GIEATKKIKQKWPTVHVVVLSSSAER--ESVIPALQAGA 99 >dbj|BAB07746.1| (AP001520) two-component response regulator [Bacillus halodurans] Length = 236 Score = 70.6 bits (170), Expect = 3e-11 Identities = 40/124 (32%), Positives = 72/124 (57%), Gaps = 9/124 (7%) Query: 9 RVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMNG 68 ++LVVDD + +LK + + +C A DG EA+E V + +PD++ LD+ +P +G Sbjct: 5 KILVVDDEKPIADILKFNLEKEGFTVIC--AYDGAEAVEQVNQEKPDLILLDIMLPNKDG 62 Query: 69 LDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEII 128 ++ R + K++ +P+IM++A E + + LE GA D++ KP S+ REL + Sbjct: 63 MEVCREVRKQFEMPIIMLTAKDSEIDK--VLGLELGADDYVTKPFST-----RELLARVK 115 Query: 129 AKIK 132 A ++ Sbjct: 116 ANLR 119 >sp|P52940|SP0A_CLOPA STAGE 0 SPORULATION PROTEIN A HOMOLOG >gi|497971|gb|AAA18883.1| (U09982) Spo0A [Clostridium pasteurianum] Length = 273 Score = 70.2 bits (169), Expect = 3e-11 Identities = 41/109 (37%), Positives = 70/109 (63%), Gaps = 5/109 (4%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI VL+ DD+ +L D + + ++ V G A+DGIEA++++++ +PD+V LD+ MP M Sbjct: 5 KISVLIADDNKEFCNILNDYLLNQSDIVVVGIAKDGIEALKLIEEKKPDLVILDIIMPNM 64 Query: 67 NGLDALRVI--MKKYPVP-VIMISALTQEGAEATIKALEYGAIDFIPKP 112 +GL L + + PVP VI++SA+ Q+ + T +A+ GA ++ KP Sbjct: 65 DGLVVLEKLANINIDPVPNVIVLSAVGQD--KITQRAITLGADYYVVKP 111 >pir||S60880 phosphorylation-activated transcription factor Spo0A - Clostridium pasteurianum Length = 274 Score = 70.2 bits (169), Expect = 3e-11 Identities = 41/109 (37%), Positives = 70/109 (63%), Gaps = 5/109 (4%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI VL+ DD+ +L D + + ++ V G A+DGIEA++++++ +PD+V LD+ MP M Sbjct: 5 KISVLIADDNKEFCNILNDYLLNQSDIVVVGIAKDGIEALKLIEEKKPDLVILDIIMPNM 64 Query: 67 NGLDALRVI--MKKYPVP-VIMISALTQEGAEATIKALEYGAIDFIPKP 112 +GL L + + PVP VI++SA+ Q+ + T +A+ GA ++ KP Sbjct: 65 DGLVVLEKLANINIDPVPNVIVLSAVGQD--KITQRAITLGADYYVVKP 111 >pir||E75450 DNA-binding response regulator - Deinococcus radiodurans (strain R1) >gi|6458715|gb|AAF10563.1|AE001951_3 (AE001951) DNA-binding response regulator [Deinococcus radiodurans] Length = 239 Score = 70.2 bits (169), Expect = 3e-11 Identities = 51/173 (29%), Positives = 83/173 (47%), Gaps = 18/173 (10%) Query: 6 RKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPR 65 R I +L+VDD +RK ++++ + +L V GEA G EA+ + PDV+ +DV MP Sbjct: 24 RPITLLLVDDHPVVRKGTRELLEGETDLRVVGEAGSGEEALAQARSLTPDVILMDVSMPG 83 Query: 66 MNGLDALRVIMKKYPVPVIMISALTQEGAEATIKA-LEYGAIDFIPKPSS---------S 115 MNG++A + I K P + + LT +A + A LE GA ++ K +S + Sbjct: 84 MNGIEATKAI--KAEQPSVGVLVLTSYDDDAYVFALLEAGAAGYLLKNASEDDLLGAVRA 141 Query: 116 ISINMRELKDEIIAKIKEAAKV------PRRFLELRRIRLLRVQKAKKVKPSV 162 ++ L I K+ E V P L R + +LRV + + + Sbjct: 142 VAAGESALHPSIARKVLERFSVASTPTPPEDDLSPRELEVLRVAASGRTNKEI 194 >sp|P13800|DEGU_BACSU TRANSCRIPTIONAL REGULATORY PROTEIN DEGU (PROTEASE PRODUCTION ENHANCER PROTEIN) >gi|72614|pir||RGBSXD extracellular proteinase response regulator degU - Bacillus subtilis >gi|143089|gb|AAA22545.1| (M21658) iep protein [Bacillus subtilis] >gi|143499|gb|AAA22733.1| (M23558) degU protein (gtg start codon) [Bacillus subtilis] >gi|143502|gb|AAA22735.1| (M23649) protein kinase (put.); putative [Bacillus subtilis] >gi|1762330|gb|AAC44938.1| (U56901) transcriptional regulator of degradation enzymes [Bacillus subtilis] >gi|2636075|emb|CAB15566.1| (Z99122) two-component response regulator [Bacillus subtilis] Length = 229 Score = 69.9 bits (168), Expect = 4e-11 Identities = 34/105 (32%), Positives = 63/105 (59%), Gaps = 1/105 (0%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 K+ ++++DD R+ +K I++ +P EV E DG EA +V+ PDVV +D+ MP + Sbjct: 3 KVNIVIIDDHQLFREGVKRILDFEPTFEVVAEGDDGDEAARIVEHYHPDVVIMDINMPNV 62 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPK 111 NG++A + +++ YP ++I ++ + T AL+ GA ++ K Sbjct: 63 NGVEATKQLVELYPESKVIILSIHDDENYVT-HALKTGARGYLLK 106 >pdb|1QMP|A Chain A, Phosphorylated Aspartate In The Crystal Structure Of The Sporulation Response Regulator, Spo0a >gi|6573583|pdb|1QMP|C Chain C, Phosphorylated Aspartate In The Crystal Structure Of The Sporulation Response Regulator, Spo0a >gi|6573584|pdb|1QMP|D Chain D, Phosphorylated Aspartate In The Crystal Structure Of The Sporulation Response Regulator, Spo0a >gi|6573582|pdb|1QMP|B Chain B, Phosphorylated Aspartate In The Crystal Structure Of The Sporulation Response Regulator, Spo0a Length = 130 Score = 69.9 bits (168), Expect = 4e-11 Identities = 40/107 (37%), Positives = 67/107 (62%), Gaps = 4/107 (3%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I+V + DD+ + +L + I+S P++EV G A +G + ++M+++ RPD++ L + MP ++ Sbjct: 3 IKVCIADDNRELVSLLDEYISSQPDMEVIGTAYNGQDCLQMLEEKRPDILLLXIIMPHLD 62 Query: 68 GLDALRVIMK--KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 GL L I ++ VIM++A QE + T KA+E GA FI KP Sbjct: 63 GLAVLERIRAGFEHQPNVIMLTAFGQE--DVTKKAVELGASYFILKP 107 >emb|CAB64972.1| (AJ012050) VicR protein [Enterococcus faecalis] Length = 283 Score = 69.9 bits (168), Expect = 4e-11 Identities = 42/128 (32%), Positives = 76/128 (58%), Gaps = 11/128 (8%) Query: 9 RVLVVDDSAFMRKVLK-DIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 ++LVVDD + +++K +++ E+ A DG EA+E V++ PD++ LD+ +P+M+ Sbjct: 52 KILVVDDEKPISEIVKYNLVKEGYEVFT---AYDGEEALEKVEEVEPDLIILDLMLPKMD 108 Query: 68 GLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDEI 127 GL+ R + K + +P+IM++A E + + LE GA D++ KP S+ REL + Sbjct: 109 GLEVAREVRKTHDMPIIMVTAKDSEIDK--VLGLELGADDYVTKPFSN-----RELVARV 161 Query: 128 IAKIKEAA 135 A ++ A Sbjct: 162 KANLRRGA 169 >sp|P52929|SP0A_BACBR STAGE 0 SPORULATION PROTEIN A >gi|2119860|pir||S60869 phosphorylation-activated transcription factor Spo0A - Bacillus brevis (fragment) >gi|497949|gb|AAA18872.1| (U09971) Spo0A [Brevibacillus brevis] Length = 212 Score = 69.5 bits (167), Expect = 6e-11 Identities = 44/109 (40%), Positives = 67/109 (61%), Gaps = 5/109 (4%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 KI VL+ DD+ +L++ I+S ++ V G A +G E + ++Q+ PDV+ LD+ MP + Sbjct: 3 KIEVLLADDNREFVSLLEEYISSQYDMNVIGVAYNGNEVVRLLQERVPDVLILDIIMPHL 62 Query: 67 NGLDALRVI--MKKYPVP-VIMISALTQEGAEATIKALEYGAIDFIPKP 112 +GL L I M+ P P +IM++A QE E T KA+E GA +I KP Sbjct: 63 DGLAVLEQIQAMRLSPQPKIIMLTAFGQE--EITKKAVELGAAYYILKP 109 >pir||T35199 probable two-component regulator - Streptomyces coelicolor >gi|3036890|emb|CAA18523.1| (AL022374) SC5B8.15, probable two-component transcriptional reg ulator, len: 229 aa; similar to many e.g. DEGU_BACSU P13800 transcriptional regulator (229 aa), fasta scores; opt: 485 z-score: 582.9 E(): 2.8e-25, 37.1% identity in 221 aa over lap. Conta> Length = 229 Score = 69.5 bits (167), Expect = 6e-11 Identities = 44/138 (31%), Positives = 73/138 (52%), Gaps = 2/138 (1%) Query: 6 RKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPR 65 R RV+VVDD +R+ + ++ P ++V G A DG EA+ +V + PDVV +D+ MPR Sbjct: 8 RPARVVVVDDQTVVREGIVMLLGLLPGVDVVGAAGDGEEAVRVVAELAPDVVLMDLRMPR 67 Query: 66 MNGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKD 125 +G++A R I ++P +++ T E+ AL GA ++ K + I +R + Sbjct: 68 CDGVEATRRIRSEHPGTQVVV-LTTYADDESLFPALHAGARGYLTKDAGGDEI-VRAVHS 125 Query: 126 EIIAKIKEAAKVPRRFLE 143 + A + RR LE Sbjct: 126 VLSGDAGLAPSIQRRLLE 143 >dbj|BAB07561.1| (AP001520) two-component response regulator [Bacillus halodurans] Length = 530 Score = 69.5 bits (167), Expect = 6e-11 Identities = 39/128 (30%), Positives = 74/128 (57%), Gaps = 5/128 (3%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 I VL+VDD R+ LK + P + + GEA +G +AIE + +PD++ +D++MP ++ Sbjct: 2 INVLIVDDEQIEREGLKLFLKDRPNIHIVGEASNGRQAIEKALETQPDLMMMDIKMPGVD 61 Query: 68 GLDALRVIMKKYP-VPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 G+ A++ I ++ P IM+SA + E + + YG +++ KP++ + D Sbjct: 62 GVQAVKEIKQQLPETEFIMVSAF--DTFEYAREVMRYGVKEYLLKPTTKEDV--LTALDR 117 Query: 127 IIAKIKEA 134 I+ +I++A Sbjct: 118 IVGEIEQA 125 >sp|P96126|CHEY_TREPA CHEMOTAXIS PROTEIN CHEY >gi|7443016|pir||D71335 probable chemotaxis response regulator (cheY) - syphilis spirochete >gi|1765976|gb|AAC45558.1| (U61851) chemotaxis protein CheY [Treponema pallidum] >gi|3322643|gb|AAC65351.1| (AE001215) chemotaxis response regulator (cheY) [Treponema pallidum] >gi|5565836|gb|AAD45223.1|AF105017_4 (AF105017) chemotaxis protein CheY [Treponema pallidum subsp. pertenue] Length = 144 Score = 69.1 bits (166), Expect = 8e-11 Identities = 46/111 (41%), Positives = 67/111 (59%), Gaps = 6/111 (5%) Query: 5 GRKIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRP--DVVTLDVE 62 G RVLVVDDS F+ K + I+ S+ EV A DG++ +E + P D+VT+D+ Sbjct: 22 GAPYRVLVVDDSMFVSKQIGQILTSEG-YEVADTAVDGVDGVEKYKAMSPGVDLVTMDIT 80 Query: 63 MPRMNGLDALRVIMK-KYPVPVIMISALTQEGAEATIKALEYGAIDFIPKP 112 MP+M+G+ AL I++ V++ISAL +E E KAL GA ++I KP Sbjct: 81 MPKMDGITALEKILEFDKNAKVVIISALGKE--ELVKKALLLGAKNYIVKP 129 >gi|11467627 ompR homolog [Guillardia theta] >gi|6136611|sp|O78428|YC27_GUITH PROBABLE TRANSCRIPTIONAL REGULATOR YCF27 (OMPR-LIKE PROTEIN) >gi|3602952|gb|AAC35613.1| (AF041468) ompR homolog [Guillardia theta] Length = 254 Score = 69.1 bits (166), Expect = 8e-11 Identities = 47/130 (36%), Positives = 71/130 (54%), Gaps = 9/130 (6%) Query: 7 KIRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRM 66 K +VL+VDD A +RK+L+ ++ V A DG EA+ + + PDVV LD+ MP++ Sbjct: 14 KEKVLIVDDEASIRKILETRLSMIGYFVVT--ASDGEEALNLFHQEMPDVVILDIMMPKL 71 Query: 67 NGLDALRVIMKKYPVPVIMISALTQEGAEATIKALEYGAIDFIPKPSSSISINMRELKDE 126 +G + I K VP+IM++AL T LE GA D++ KP S +EL+ Sbjct: 72 DGYGVCQEIRKDSDVPIIMLTALGDVADRIT--GLELGADDYVVKPFSP-----KELEAR 124 Query: 127 IIAKIKEAAK 136 I A ++ K Sbjct: 125 IRAVLRRTNK 134 >pir||T36543 probable two-component system regulator - Streptomyces coelicolor >gi|4753877|emb|CAB42040.1| (AL049754) putative two-component system regulator [Streptomyces coelicolor A3(2)] Length = 248 Score = 69.1 bits (166), Expect = 8e-11 Identities = 41/105 (39%), Positives = 58/105 (55%), Gaps = 1/105 (0%) Query: 8 IRVLVVDDSAFMRKVLKDIINSDPELEVCGEARDGIEAIEMVQKCRPDVVTLDVEMPRMN 67 IR+LVVDD +R L ++ + P +EV GEA DG EA+ + + RPDVV +D+ MP + Sbjct: 19 IRLLVVDDQYLVRTGLATLLRAAPGMEVVGEASDGAEAVVLAARTRPDVVLMDIRMPGLT 78 Query: 68 GLDALRVIMKKYPVPVIMISALTQ-EGAEATIKALEYGAIDFIPK 111 G+ A I+ + P I LT + E AL GA F+ K Sbjct: 79 GIQATERILAQAVDPAPRILVLTTFDLDEYVYGALRAGASGFLLK 123 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.320 0.138 0.386 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 128776504 Number of Sequences: 2977 Number of extensions: 5293071 Number of successful extensions: 13959 Number of sequences better than 1.0e-10: 101 Number of HSP's better than 0.0 without gapping: 40 Number of HSP's successfully gapped in prelim test: 61 Number of HSP's that attempted gapping in prelim test: 13835 Number of HSP's gapped (non-prelim): 107 length of query: 368 length of database: 189,106,746 effective HSP length: 58 effective length of query: 310 effective length of database: 154,394,500 effective search space: 47862295000 effective search space used: 47862295000 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.8 bits) S2: 166 (69.1 bits)