BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1333 (cheA-like) DE:CHEA HISTIDINE KINASE related protein (212 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||C75002 chea histidine kinase related protein PAB1333 - Pyro... 418 e-116 pir||B71161 hypothetical protein PH0487 - Pyrococcus horikoshii ... 369 e-101 gi|11498644 conserved hypothetical protein [Archaeoglobus fulgid... 100 2e-20 dbj|BAB06153.1| (AP001515) inhibition of CheR-mediated methylati... 99 4e-20 pir||G72318 chemotaxis protein CheC - Thermotoga maritima (strai... 96 2e-19 pir||C71161 hypothetical protein PH0488 - Pyrococcus horikoshii ... 95 5e-19 pir||B75002 chea histidine kinase PAB1334 - Pyrococcus abyssi (s... 91 1e-17 sp|P40403|CHEC_BACSU CHEMOTAXIS PROTEIN CHEC >gi|1075909|pir||A5... 78 1e-13 >pir||C75002 chea histidine kinase related protein PAB1333 - Pyrococcus abyssi (strain Orsay) >gi|5458971|emb|CAB50457.1| (AJ248288) CHEA HISTIDINE KINASE related protein [Pyrococcus abyssi] Length = 212 Score = 418 bits (1062), Expect = e-116 Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 1 MDIVAENIEEYVKNLDEFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKN 60 MDIVAENIEEYVKNLDEFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKN Sbjct: 1 MDIVAENIEEYVKNLDEFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKN 60 Query: 61 VPEIVGEDVKVVVYIELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSF 120 VPEIVGEDVKVVVYIELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSF Sbjct: 61 VPEIVGEDVKVVVYIELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSF 120 Query: 121 MEMGNILISAFANALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDI 180 MEMGNILISAFANALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDI Sbjct: 121 MEMGNILISAFANALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDI 180 Query: 181 KISGVEFEEHFLLFPKPEDMKKILEKLLGGLA 212 KISGVEFEEHFLLFPKPEDMKKILEKLLGGLA Sbjct: 181 KISGVEFEEHFLLFPKPEDMKKILEKLLGGLA 212 >pir||B71161 hypothetical protein PH0487 - Pyrococcus horikoshii >gi|3256892|dbj|BAA29575.1| (AP000002) 212aa long hypothetical protein [Pyrococcus horikoshii] Length = 212 Score = 369 bits (937), Expect = e-101 Identities = 182/212 (85%), Positives = 198/212 (92%) Query: 1 MDIVAENIEEYVKNLDEFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKN 60 MDIV E EEY+KNLDE +KSALLETFNIGASHAATALSEMTG+EVNISVPDLK++ IKN Sbjct: 1 MDIVDEKWEEYLKNLDEMSKSALLETFNIGASHAATALSEMTGKEVNISVPDLKIIAIKN 60 Query: 61 VPEIVGEDVKVVVYIELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSF 120 VPE+VGEDVKV VYIELGKDFSSHAFF ADY+D LRMFD+IMGNP GTT E+D+MVKSSF Sbjct: 61 VPEVVGEDVKVAVYIELGKDFSSHAFFIADYEDALRMFDIIMGNPLGTTKEMDEMVKSSF 120 Query: 121 MEMGNILISAFANALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDI 180 ME+GNILISAFANALS+FLGITIEQTPP+L IDFLPAILD ALADIGK+CDYTIILQT+I Sbjct: 121 MEVGNILISAFANALSEFLGITIEQTPPSLAIDFLPAILDFALADIGKYCDYTIILQTNI 180 Query: 181 KISGVEFEEHFLLFPKPEDMKKILEKLLGGLA 212 KIS V+FEEHFLLFPKPEDMKKILEKLLGG A Sbjct: 181 KISDVKFEEHFLLFPKPEDMKKILEKLLGGFA 212 >gi|11498644 conserved hypothetical protein [Archaeoglobus fulgidus] >gi|7483078|pir||G69379 conserved hypothetical protein AF1039 - Archaeoglobus fulgidus >gi|2649551|gb|AAB90201.1| (AE001031) conserved hypothetical protein [Archaeoglobus fulgidus] Length = 205 Score = 100 bits (246), Expect = 2e-20 Identities = 60/202 (29%), Positives = 108/202 (52%), Gaps = 10/202 (4%) Query: 12 VKNLDEFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKNVPEIVGEDVKV 71 +++ E AL E NIG AAT+LS+M GR + +SVP+ +V+I + + + + V Sbjct: 7 IEDFGEMELDALKELGNIGVGKAATSLSQMLGRRIEMSVPEAVIVKIPELHKYINAEEMV 66 Query: 72 VVYI----ELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSFMEMGNIL 127 + ++ S + ++ ++++G+ ++MV S+ ME+GNIL Sbjct: 67 AGVVTGLEDIENGHSGFLYITFPEKSSKKLAEILLGDSSD-----EEMVDSTLMEIGNIL 121 Query: 128 ISAFANALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDIKISGVEF 187 S+F +A ++ LGI + TPP+ DF A++D ++ + + DY +I +T ++ S E Sbjct: 122 SSSFCDAAAEMLGIVLLPTPPSFGRDFAIAVIDAVVSQLAEKSDYVVIFETKLEESENEI 181 Query: 188 EEHFLLFPKPEDMKKILEKLLG 209 E +L P + + IL KLLG Sbjct: 182 EALVMLIPNEKFVSYIL-KLLG 202 >dbj|BAB06153.1| (AP001515) inhibition of CheR-mediated methylation of MCPs [Bacillus halodurans] Length = 209 Score = 99.1 bits (243), Expect = 4e-20 Identities = 62/206 (30%), Positives = 104/206 (50%), Gaps = 4/206 (1%) Query: 10 EYVKNLDEFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKNVPEIVG--E 67 E + L F L E NIGA +AATALS++ + ++++VP++ +V + + E +G E Sbjct: 2 ENLSELQAFHLDILKEIGNIGAGNAATALSQLLQKTIDMNVPNVHIVSFQELTEWIGGPE 61 Query: 68 DVKVVVYIELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTT-ELDDMVKSSFMEMGNI 126 +V ++ + D FF A ++ M + G + E+DD+ S+ E+GNI Sbjct: 62 EVVAATFLRIEGDAPGSMFFLATLEEAKGMVAQLTGASRSSLEGEMDDLSMSALKELGNI 121 Query: 127 LISAFANALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDIKISGV- 185 L ++ +AL+ F + + + P L+ID + AIL+ L + + D I++ T I G Sbjct: 122 LAGSYLSALADFTKLKLYPSVPGLSIDMIGAILNYGLIPLSQIGDEAIVIDTQISEEGER 181 Query: 186 EFEEHFLLFPKPEDMKKILEKLLGGL 211 HF L P PE + L GL Sbjct: 182 RGAGHFFLLPDPESFDVMFSSLGVGL 207 >pir||G72318 chemotaxis protein CheC - Thermotoga maritima (strain MSB8) >gi|4981440|gb|AAD35985.1|AE001755_8 (AE001755) chemotaxis protein CheC [Thermotoga maritima] Length = 205 Score = 96.3 bits (236), Expect = 2e-19 Identities = 62/201 (30%), Positives = 103/201 (50%), Gaps = 10/201 (4%) Query: 15 LDEFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKNVPEIVGEDVKVVVY 74 + E K L E NIGA +AATA+S M ++V ISVP++++V I V I + ++VV Sbjct: 3 ISERQKDLLKEIGNIGAGNAATAISYMINKKVEISVPNVEIVPISKVIFIAKDPEEIVVG 62 Query: 75 IELGK--DFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSFMEMGNILISAFA 132 +++ D V ++ +++ G P LD+ S+ E+GNI+ + Sbjct: 63 VKMPVTGDIEGSVLLIMGTTVVKKILEILTGRAPDNLLNLDEFSASALREIGNIMCGTYV 122 Query: 133 NALSQFLGITIEQTPPALTIDFLPAILDLA----LADIGKFCDYTIILQTDIKISGVE-- 186 +AL+ FLG I+ PP L ID + AI A L D + D + ++T +K+ E Sbjct: 123 SALADFLGFKIDTLPPQLVIDMISAIFAEASIEELEDNSE--DQIVFVETLLKVEEEEEP 180 Query: 187 FEEHFLLFPKPEDMKKILEKL 207 + ++ PKP + KI E++ Sbjct: 181 LTSYMMMIPKPGYLVKIFERM 201 >pir||C71161 hypothetical protein PH0488 - Pyrococcus horikoshii >gi|3256893|dbj|BAA29576.1| (AP000002) 211aa long hypothetical protein [Pyrococcus horikoshii] Length = 211 Score = 95.2 bits (233), Expect = 5e-19 Identities = 57/194 (29%), Positives = 93/194 (47%), Gaps = 4/194 (2%) Query: 17 EFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKNVPEIVGE---DVKVVV 73 E K E NI SHA TALS+M G + + PD+ +V +I+ E V Sbjct: 5 ELYKDIFKEASNIAMSHALTALSQMIGGPIEMEAPDVDIVSRVEFLKILAERGVSKGFTV 64 Query: 74 YIELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSFMEMGNILISAFAN 133 ++ + S L + V++G PG+ TELD+M KS+ ME+GNILIS + + Sbjct: 65 MFDITEGMSGLTILQFPRQSALNISSVLLGMEPGSMTELDEMGKSAIMEVGNILISVYTD 124 Query: 134 ALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDIKISGVEFEEHFLL 193 LS LG + +PP +L +D+ + + ++ ++ + E +F + Sbjct: 125 ILSNLLGEPVSLSPPKPAESLYDIEKELGRSDL-RNVNSVVVFKSKFYKEDIRVESYFYI 183 Query: 194 FPKPEDMKKILEKL 207 P PE K+++KL Sbjct: 184 IPTPESFTKLVKKL 197 >pir||B75002 chea histidine kinase PAB1334 - Pyrococcus abyssi (strain Orsay) >gi|5458970|emb|CAB50456.1| (AJ248288) CHEA HISTIDINE KINASE [Pyrococcus abyssi] Length = 212 Score = 90.9 bits (222), Expect = 1e-17 Identities = 56/194 (28%), Positives = 93/194 (47%), Gaps = 4/194 (2%) Query: 17 EFAKSALLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKNVPEIVGE---DVKVVV 73 E K E NI SHA T+LS+M G + + PD+++V +++ E V Sbjct: 5 ELYKDIFKEASNIAMSHALTSLSQMIGGPIEMEAPDVEIVSRAEFLKLLAERGVSKGFTV 64 Query: 74 YIELGKDFSSHAFFAADYDDVLRMFDVIMGNPPGTTTELDDMVKSSFMEMGNILISAFAN 133 ++ + S L + V+MG PG+ ELD+M KS+ ME+GNILISA+ + Sbjct: 65 MFDVTEGLSGLTILQFPKHSALNISAVLMGMEPGSMEELDEMGKSAIMEVGNILISAYTD 124 Query: 134 ALSQFLGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDIKISGVEFEEHFLL 193 LS +G + +PP +L D+ + + ++ ++ + E +F L Sbjct: 125 ILSNLIGEPVSLSPPKPAESLYDIEKELGRPDL-RNVNSIVVFKSKFYKEDIGVESYFYL 183 Query: 194 FPKPEDMKKILEKL 207 P PE K+++KL Sbjct: 184 VPTPESFTKLVKKL 197 >sp|P40403|CHEC_BACSU CHEMOTAXIS PROTEIN CHEC >gi|1075909|pir||A55216 chemotactic methyltransferase inhibitor protein - Bacillus subtilis >gi|289302|gb|AAA61468.1| (M20144) ORFA [Bacillus subtilis] >gi|2634017|emb|CAB13518.1| (Z99112) alternate gene name: ylxJ [Bacillus subtilis] Length = 209 Score = 77.6 bits (188), Expect = 1e-13 Identities = 46/191 (24%), Positives = 96/191 (50%), Gaps = 6/191 (3%) Query: 23 LLETFNIGASHAATALSEMTGREVNISVPDLKVVEIKNVPEIVG-EDVKVV-VYIELGKD 80 L E NIGA H+A+A++++ R++++ VP K++ + + G DV V +++ + D Sbjct: 15 LREVGNIGAGHSASAMAQLLNRKIDMEVPFAKLLSFDELVDFFGGADVPVASIFLRMEGD 74 Query: 81 FSSHAFFAADYDDVLRMFDVIMGNPPGTTTEL--DDMVKSSFMEMGNILISAFANALSQF 138 + FF + + ++GNP +L D M S+ E+GNIL ++ AL+ Sbjct: 75 LTGSMFFIMPFFQAEQFIRELIGNPDFDIEDLGEDHMSSSALHELGNILAGSYLTALADL 134 Query: 139 LGITIEQTPPALTIDFLPAILDLALADIGKFCDYTIILQTDI--KISGVEFEEHFLLFPK 196 + + + P +++D A++ L ++ + ++ I++ T I + E + H + P Sbjct: 135 TKLQLYPSVPEVSLDMFGAVISEGLMELSQVGEHAIVVDTSIFDQSHQQELKAHMFMLPD 194 Query: 197 PEDMKKILEKL 207 + +K+ L Sbjct: 195 YDSFEKLFVAL 205 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.320 0.139 0.385 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 74144813 Number of Sequences: 2977 Number of extensions: 2843093 Number of successful extensions: 6772 Number of sequences better than 1.0e-10: 8 Number of HSP's better than 0.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 6750 Number of HSP's gapped (non-prelim): 12 length of query: 212 length of database: 189,106,746 effective HSP length: 56 effective length of query: 156 effective length of database: 155,591,474 effective search space: 24272269944 effective search space used: 24272269944 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.8 bits) S2: 163 (67.9 bits)