BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1335 (cheD) DE:CHEMOTAXIS PROTEIN CHED (163 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||A75002 chemotaxis protein ched PAB1335 - Pyrococcus abyssi ... 324 4e-88 pir||E71161 hypothetical protein PH0490 - Pyrococcus horikoshii ... 299 1e-80 pir||F72318 chemotaxis methylation protein - Thermotoga maritima... 145 4e-34 gi|11498643 conserved hypothetical protein [Archaeoglobus fulgid... 130 1e-29 dbj|BAB06152.1| (AP001515) methylation of methyl-accepting chemo... 127 5e-29 sp|P40404|CHED_BACSU CHEMOTAXIS PROTEIN CHED >gi|1075910|pir||B5... 115 3e-25 emb|CAA07182.1| (AJ006687) CheD [Caulobacter crescentus] 90 2e-17 pir||S61839 hypothetical protein 9 - Rhizobium meliloti >gi|5348... 85 6e-16 gb|AAC25081.1| (AF044495) unknown [Agrobacterium tumefaciens] 82 5e-15 pir||G83623 conserved hypothetical protein PA0174 [imported] - P... 80 2e-14 emb|CAB87132.1| (X80205) CheD protein [Rhodobacter sphaeroides] 77 9e-14 pir||F82379 probable chemotaxis protein CheD VCA1090 [imported] ... 74 1e-12 gb|AAG19390.1| (AE005033) chemotaxis protein; CheD [Halobacteriu... 74 1e-12 >pir||A75002 chemotaxis protein ched PAB1335 - Pyrococcus abyssi (strain Orsay) >gi|5458969|emb|CAB50455.1| (AJ248288) CHEMOTAXIS PROTEIN CHED [Pyrococcus abyssi] Length = 163 Score = 324 bits (821), Expect = 4e-88 Identities = 163/163 (100%), Positives = 163/163 (100%) Query: 1 MTREIKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGN 60 MTREIKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGN Sbjct: 1 MTREIKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGN 60 Query: 61 PAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELK 120 PAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELK Sbjct: 61 PAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELK 120 Query: 121 KLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQVIEKVY 163 KLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQVIEKVY Sbjct: 121 KLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQVIEKVY 163 >pir||E71161 hypothetical protein PH0490 - Pyrococcus horikoshii >gi|3256895|dbj|BAA29578.1| (AP000002) 162aa long hypothetical protein [Pyrococcus horikoshii] Length = 162 Score = 299 bits (757), Expect = 1e-80 Identities = 147/161 (91%), Positives = 156/161 (96%) Query: 3 REIKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNPA 62 +E+KVGIGDYAVGKG GIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNPA Sbjct: 2 KEVKVGIGDYAVGKGNGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNPA 61 Query: 63 KYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELKKL 122 KYVDTGLQLLLKEV+KLGASKFRLEAKLFGGAQMF NI+S+ELKIGE+NVQ AKREL+KL Sbjct: 62 KYVDTGLQLLLKEVIKLGASKFRLEAKLFGGAQMFDNIRSEELKIGEKNVQVAKRELRKL 121 Query: 123 GIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQVIEKVY 163 GIRLVAEDTGG+GGRTIYLDLSTGKVRMRKV G+VIEKVY Sbjct: 122 GIRLVAEDTGGKGGRTIYLDLSTGKVRMRKVTEGKVIEKVY 162 >pir||F72318 chemotaxis methylation protein - Thermotoga maritima (strain MSB8) >gi|4981439|gb|AAD35984.1|AE001755_7 (AE001755) chemotaxis methylation protein [Thermotoga maritima] Length = 157 Score = 145 bits (361), Expect = 4e-34 Identities = 73/154 (47%), Positives = 103/154 (66%), Gaps = 5/154 (3%) Query: 7 VGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNPAKYVD 66 +GIG+YAV K G+I T GLGSCV + + D V KVG + H +LP++ G P KY D Sbjct: 5 IGIGEYAVMKNPGVIVTLGLGSCVAVCMRDPVAKVGAMAHVMLPDSG--GKTDKPGKYAD 62 Query: 67 TGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELKKLGIRL 126 T ++ L++E+ K+GA RLEAK+ GGA MF+ S + IG RNV+ K+ LK GI+L Sbjct: 63 TAVKTLVEELKKMGAKVERLEAKIAGGASMFE---SKGMNIGARNVEAVKKHLKDFGIKL 119 Query: 127 VAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQVIE 160 +AEDTGG R++ ++ TGK+ +RKV GG+ +E Sbjct: 120 LAEDTGGNRARSVEYNIETGKLLVRKVGGGEQLE 153 >gi|11498643 conserved hypothetical protein [Archaeoglobus fulgidus] >gi|7448490|pir||F69379 conserved hypothetical protein AF1038 - Archaeoglobus fulgidus >gi|2649552|gb|AAB90202.1| (AE001031) conserved hypothetical protein [Archaeoglobus fulgidus] Length = 157 Score = 130 bits (323), Expect = 1e-29 Identities = 65/158 (41%), Positives = 104/158 (65%), Gaps = 3/158 (1%) Query: 1 MTREIKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGN 60 M EI VGIG++ V KG ++ T GLGSCVGI LYD ++GGL H +LP++ R Sbjct: 1 MGSEILVGIGEFRVAKG-AVLKTIGLGSCVGIALYDPKLRLGGLAHVMLPQSGNGTKRS- 58 Query: 61 PAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELK 120 AKY D ++++ + + +LG+ + R+ AK+ GGAQ+F+++ D L+IG+RN + + L+ Sbjct: 59 -AKYADHAVEMMTEAMERLGSDRKRIVAKMAGGAQIFKHMTMDMLRIGDRNAEAIRTILR 117 Query: 121 KLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQV 158 GIR+V+ED GG GRT+Y + G++ ++ GG++ Sbjct: 118 DYGIRIVSEDLGGDEGRTVYFFTNDGRMLVKYSRGGEL 155 >dbj|BAB06152.1| (AP001515) methylation of methyl-accepting chemotaxis protein [Bacillus halodurans] Length = 162 Score = 127 bits (317), Expect = 5e-29 Identities = 70/159 (44%), Positives = 100/159 (62%), Gaps = 2/159 (1%) Query: 1 MTREIKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAA-RYGHRG 59 M +KVG+ D V I T GLGSCVG+ LYD V KV G+ H +LP++A Sbjct: 1 MNDVVKVGMADLNVVTPPHTIRTAGLGSCVGVVLYDDVKKVAGMAHIMLPDSALGKKQEF 60 Query: 60 NPAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQ-NIKSDELKIGERNVQTAKRE 118 N AKY DT L LLL ++ +GA ++ L+AK+ GGAQMF +D ++IG+RNV+ K++ Sbjct: 61 NRAKYADTALDLLLSKLEAIGAKRYSLKAKMAGGAQMFSFASNNDMMRIGQRNVEAVKKK 120 Query: 119 LKKLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQ 157 L++L I ++AED GG GRTI + T ++ +R V G+ Sbjct: 121 LRELSIPILAEDVGGSNGRTIEFNPETKRLSIRTVHQGE 159 >sp|P40404|CHED_BACSU CHEMOTAXIS PROTEIN CHED >gi|1075910|pir||B55216 chemotaxis methylation system protein cheD - Bacillus subtilis >gi|289303|gb|AAA61469.1| (M20144) ORFB [Bacillus subtilis] >gi|2634018|emb|CAB13519.1| (Z99112) alternate gene name: ylxK [Bacillus subtilis] Length = 166 Score = 115 bits (285), Expect = 3e-25 Identities = 63/155 (40%), Positives = 92/155 (58%), Gaps = 2/155 (1%) Query: 5 IKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAA-RYGHRGNPAK 63 IKVGI D + + I T GLGSCVG+ LYD+ + GL+H +LP++ N AK Sbjct: 9 IKVGIADVKIARFPDTIRTSGLGSCVGLVLYDKEKQTAGLVHVMLPDSTLSKTAELNRAK 68 Query: 64 YVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQ-NIKSDELKIGERNVQTAKRELKKL 122 Y DT +Q + +++ G KF L+AKL GG++MF+ +D +KIG RNV K +L Sbjct: 69 YADTAVQTTIDMLIEAGCRKFALKAKLAGGSEMFKFKSTNDLMKIGPRNVLAIKEQLSLF 128 Query: 123 GIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIGGQ 157 I +++EDTGG GRTI + + + +R V G+ Sbjct: 129 NIPIISEDTGGSSGRTIEFEPKSCMLHIRTVKQGE 163 >emb|CAA07182.1| (AJ006687) CheD [Caulobacter crescentus] Length = 187 Score = 89.7 bits (219), Expect = 2e-17 Identities = 52/153 (33%), Positives = 85/153 (54%), Gaps = 6/153 (3%) Query: 3 REIKVGI--GDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGN 60 R +KV + G+ V ++ T LGSC+ + D + VGG+ H LLP++ G+ Sbjct: 11 RAVKVHVTQGESHVTADPNVVMTTVLGSCIAACIRDPQSGVGGMNHFLLPDSGDGRRDGD 70 Query: 61 PAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELK 120 +Y +++L+ ++LK GA + RLEAK+FGGA++F + +G N A+R L+ Sbjct: 71 AVRYGAYAMEVLINDLLKRGARRERLEAKIFGGAKLFDGLSD----VGASNAAFAERFLR 126 Query: 121 KLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKV 153 GI +V+ TGG R + ++G+VR R V Sbjct: 127 DEGIPIVSSSTGGVSARRVEFWPASGRVRQRLV 159 >pir||S61839 hypothetical protein 9 - Rhizobium meliloti >gi|534842|gb|AAA86679.1| (U13166) ORF9 [Sinorhizobium meliloti] Length = 183 Score = 84.7 bits (206), Expect = 6e-16 Identities = 56/154 (36%), Positives = 82/154 (52%), Gaps = 5/154 (3%) Query: 3 REIKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNPA 62 R + V G++ V I+ + LGSCV + D VGG+ H LLP +A G A Sbjct: 8 RRVHVIQGEFKVVNDPDIVLSTILGSCVAACMRDPAAGVGGMNHFLLPGSATSPSSGADA 67 Query: 63 -KYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELKK 121 +Y ++LL+ +LK GA + RLEAK+FGGA+ + +GE+N A++ L Sbjct: 68 TRYGVHLMELLINGLLKQGARRDRLEAKIFGGARTIARFSN----VGEQNAAFARQFLMD 123 Query: 122 LGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKVIG 155 GIR+V E TGG GR + S+G+ R + G Sbjct: 124 EGIRIVGESTGGDHGRKLEYWPSSGRARQYALTG 157 >gb|AAC25081.1| (AF044495) unknown [Agrobacterium tumefaciens] Length = 181 Score = 81.5 bits (198), Expect = 5e-15 Identities = 53/153 (34%), Positives = 80/153 (51%), Gaps = 5/153 (3%) Query: 10 GDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNPAKYVDTGL 69 G+Y V ++ T LGSCV L D V +GG+ H LLP G+ +Y + Sbjct: 14 GEYKVVSDPDVVMTTILGSCVAACLRDPVAGLGGMNHFLLPGTGNV-TGGDATRYGVHLM 72 Query: 70 QLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELKKLGIRLVAE 129 +LL+ +LK GA + RLEAK+FGGA+ + + +GE+N A + LK GI +++ Sbjct: 73 ELLINGLLKQGARRDRLEAKVFGGAKTIASFSN----VGEQNAIFAMQFLKDEGIPVISS 128 Query: 130 DTGGRGGRTIYLDLSTGKVRMRKVIGGQVIEKV 162 TGG GR I +G+ R + G + + V Sbjct: 129 STGGDHGRKIEFWPVSGRARQHPLSGAETQKTV 161 >pir||G83623 conserved hypothetical protein PA0174 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9946007|gb|AAG03564.1|AE004455_5 (AE004455) conserved hypothetical protein [Pseudomonas aeruginosa] Length = 200 Score = 79.6 bits (193), Expect = 2e-14 Identities = 51/151 (33%), Positives = 81/151 (52%), Gaps = 5/151 (3%) Query: 5 IKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNP-AK 63 +K+ G+Y GE ++ LGSCV + L D + + G+ H +LPE G +P A+ Sbjct: 24 VKLLPGEY-FASGEDLVIVTVLGSCVSVCLRDPLNGIAGMNHFMLPERGLGGDPASPSAR 82 Query: 64 YVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELKKLG 123 Y ++LL+ +L LGAS+ RL+AK+FGG + + I + IG+RNV+ L Sbjct: 83 YGSHAMELLINRMLALGASRERLQAKVFGGGSVLRQISA---AIGQRNVEFTLDYLATER 139 Query: 124 IRLVAEDTGGRGGRTIYLDLSTGKVRMRKVI 154 I L+A D R IY G+V ++ ++ Sbjct: 140 IPLLASDVLEAFPRKIYFFPRNGRVLVKSLV 170 >emb|CAB87132.1| (X80205) CheD protein [Rhodobacter sphaeroides] Length = 199 Score = 77.3 bits (187), Expect = 9e-14 Identities = 48/124 (38%), Positives = 67/124 (53%), Gaps = 6/124 (4%) Query: 26 LGSCVGITLYDRVTKVGGLLHALLPEAARYGHRGNPAKYVDTGLQLLLKEVLKLGASKFR 85 LGSC+ + D V VGG+ H LLP A +Y ++LL+ +LKLGA++ R Sbjct: 37 LGSCICTCMCDPVAGVGGMNHFLLPSADV--EDAQHLRYGSHAMELLINALLKLGAARQR 94 Query: 86 LEAKLFGGAQMFQNIKSDELKIGERNVQTAKRELKKLGIRLVAEDTGGRGGRTIYLDLST 145 +EAK+FGGA M + + IG+ N A+R L+ GIR A GG R I T Sbjct: 95 IEAKIFGGAMMTPQLGA----IGQANAAFARRYLRDEGIRCTAHSLGGNRARRIRFWPKT 150 Query: 146 GKVR 149 G+V+ Sbjct: 151 GRVQ 154 >pir||F82379 probable chemotaxis protein CheD VCA1090 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9658536|gb|AAF96982.1| (AE004434) chemotaxis protein CheD, putative [Vibrio cholerae] Length = 233 Score = 73.7 bits (178), Expect = 1e-12 Identities = 49/159 (30%), Positives = 84/159 (52%), Gaps = 17/159 (10%) Query: 5 IKVGIGDYAVGKGEGIISTYGLGSCVGITLYDRVTKVGGLLHALLPEAARYGHR------ 58 +KV G + II T GLGSCV +D KVGG+ H LLP ++ + Sbjct: 50 VKVMPGGVYATSDQEIIHT-GLGSCVSACAWDIEMKVGGMNHFLLPFNNQFESQHWHPQA 108 Query: 59 --GNPAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQM--FQNIKSDELKIGERNVQT 114 + ++Y +++L+ +L +GA + RL+ KLFGGA + +Q++ +GE+NV+ Sbjct: 109 LLSDSSRYGCYAMEVLINRLLSMGAERERLKFKLFGGAHLMGYQSL------VGEKNVEF 162 Query: 115 AKRELKKLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKV 153 K+ + +VA+D GG R + D TG+ ++++ Sbjct: 163 VLEYAKREKLNVVAQDLGGAQPRKLLFDPQTGQAWVKRI 201 >gb|AAG19390.1| (AE005033) chemotaxis protein; CheD [Halobacterium sp. NRC-1] Length = 109 Score = 73.7 bits (178), Expect = 1e-12 Identities = 45/106 (42%), Positives = 61/106 (57%), Gaps = 4/106 (3%) Query: 48 LLPEAARYGHRGNPAKYVDTGLQLLLKEVLKLGASKFRLEAKLFGGAQMFQNIKSDELKI 107 +LPEA G PAKYVD+GL ++ +L+LGAS L AKL GG+ M D + Sbjct: 1 MLPEAD--GPVDTPAKYVDSGLDEMVSALLRLGASPSTLTAKLAGGSTMLDLSIGD--SV 56 Query: 108 GERNVQTAKRELKKLGIRLVAEDTGGRGGRTIYLDLSTGKVRMRKV 153 G RNV A+R L I L+A +TGG GR++ S+G V + +V Sbjct: 57 GSRNVSAAERALADADIELLASETGGDSGRSVSFSPSSGDVTIDRV 102 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.319 0.142 0.397 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 60694468 Number of Sequences: 2977 Number of extensions: 2533819 Number of successful extensions: 5745 Number of sequences better than 1.0e-10: 13 Number of HSP's better than 0.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 5721 Number of HSP's gapped (non-prelim): 13 length of query: 163 length of database: 189,106,746 effective HSP length: 54 effective length of query: 109 effective length of database: 156,788,448 effective search space: 17089940832 effective search space used: 17089940832 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 162 (67.5 bits)