BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1440 (alaS-frag) DE:ALANYL-TRNA SYNTHETASE fragment
(159 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||A75053 alanine--tRNA ligase truncated homolog PAB1440 - Pyr... 321 2e-87
pir||B71172 alanine--tRNA ligase truncated homolog PH0574 - Pyro... 278 3e-74
gb|AAK41508.1| Alanyl-tRNA synthetase truncated homolog (alaS-li... 185 2e-46
pir||B75017 alanyl-tRNA synthetase (alas) PAB1245 - Pyrococcus a... 90 2e-17
sp|Q57984|SYA_METJA ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE... 84 7e-16
gi|11499836 alanyl-tRNA synthetase (alaS) [Archaeoglobus fulgidu... 84 7e-16
sp|O58035|SYA_PYRHO ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE... 83 2e-15
gb|AAK40671.1| Alanyl-tRNA synthetase (alaS) [Sulfolobus solfata... 81 6e-15
gb|AAG23137.1|AF245445_1 (AF245445) alanyl-tRNA synthetase [Giar... 78 7e-14
gb|AAF05594.1|AF188719_1 (AF188719) alanyl-tRNA synthetase [Giar... 76 2e-13
sp|O27718|SYA_METTH ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE... 76 3e-13
sp|Q9Y9X3|SYA_AERPE ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE... 73 1e-12
>pir||A75053 alanine--tRNA ligase truncated homolog PAB1440 - Pyrococcus abyssi
(strain Orsay) >gi|5458831|emb|CAB50318.1| (AJ248287)
ALANYL-TRNA SYNTHETASE fragment [Pyrococcus abyssi]
Length = 159
Score = 321 bits (815), Expect = 2e-87
Identities = 159/159 (100%), Positives = 159/159 (100%)
Query: 1 MVAVDVEVRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRI 60
MVAVDVEVRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRI
Sbjct: 1 MVAVDVEVRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRI 60
Query: 61 EELANEKVRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNK 120
EELANEKVRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNK
Sbjct: 61 EELANEKVRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNK 120
Query: 121 EHVKRTGEIGKIKIRKVRFRKSKGLLEVHFDVLEFEDPS 159
EHVKRTGEIGKIKIRKVRFRKSKGLLEVHFDVLEFEDPS
Sbjct: 121 EHVKRTGEIGKIKIRKVRFRKSKGLLEVHFDVLEFEDPS 159
>pir||B71172 alanine--tRNA ligase truncated homolog PH0574 - Pyrococcus
horikoshii >gi|3256980|dbj|BAA29663.1| (AP000002) 157aa
long hypothetical alanyl-tRNA synthetase [Pyrococcus
horikoshii]
Length = 157
Score = 278 bits (703), Expect = 3e-74
Identities = 132/154 (85%), Positives = 143/154 (92%)
Query: 6 VEVRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELAN 65
+EVRTHSALHVVKGAVVKVLG AKWTYSTYVKGN+GVL VKFDRKPS+EEIR IE LAN
Sbjct: 4 IEVRTHSALHVVKGAVVKVLGSEAKWTYSTYVKGNKGVLIVKFDRKPSDEEIREIERLAN 63
Query: 66 EKVRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKR 125
EKV+EN PIK+YELPREEAEK FGE+MYDLFPVPEDV+ILKVVVIEDWNVNACNKEH K
Sbjct: 64 EKVKENAPIKIYELPREEAEKMFGEDMYDLFPVPEDVRILKVVVIEDWNVNACNKEHTKT 123
Query: 126 TGEIGKIKIRKVRFRKSKGLLEVHFDVLEFEDPS 159
TGEIG IKIRKVRFRKSKGLLE+HF++LE E+PS
Sbjct: 124 TGEIGPIKIRKVRFRKSKGLLEIHFELLELENPS 157
>gb|AAK41508.1| Alanyl-tRNA synthetase truncated homolog (alaS-like2) [Sulfolobus
solfataricus]
Length = 150
Score = 185 bits (465), Expect = 2e-46
Identities = 90/147 (61%), Positives = 117/147 (79%), Gaps = 2/147 (1%)
Query: 6 VEVRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELAN 65
VE+RTH+ALHVVKGAV KVLG AKWT STYV N G LTVKF+RKPS++E+ ++ ELAN
Sbjct: 4 VEIRTHTALHVVKGAVRKVLG--AKWTASTYVNSNHGRLTVKFERKPSDQEMDKVFELAN 61
Query: 66 EKVRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKR 125
EKVR+N+PI + LPREEAE+++G+E+YDLF + +V+ L +VVI DW++NACNK+H K
Sbjct: 62 EKVRKNLPIIIEVLPREEAERKYGDEIYDLFLISAEVRELSIVVIPDWSINACNKQHTKF 121
Query: 126 TGEIGKIKIRKVRFRKSKGLLEVHFDV 152
T EIG+I R+R SK LLE+ FD+
Sbjct: 122 TSEIGEIIKDYWRYRNSKQLLEISFDI 148
>pir||B75017 alanyl-tRNA synthetase (alas) PAB1245 - Pyrococcus abyssi (strain
Orsay) >gi|5459090|emb|CAB50576.1| (AJ248288)
alanyl-tRNA synthetase (alaS) [Pyrococcus abyssi]
Length = 914
Score = 89.7 bits (219), Expect = 2e-17
Identities = 50/127 (39%), Positives = 76/127 (59%), Gaps = 3/127 (2%)
Query: 8 VRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEK 67
+R H+ HV+ GA+V+VLG+ W + + + L + ++ S+EE+R IE LAN
Sbjct: 610 MRHHTGTHVLMGALVRVLGKHV-WQAGSQLTTDWARLDISHYKRISDEELREIERLANRI 668
Query: 68 VRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTG 127
V E+ + LPR EAE+R+G +Y VP ++++VV IEDW+V AC H+ TG
Sbjct: 669 VMEDRKVTWEWLPRTEAEQRYGFRLYQGGVVPG--RVIRVVKIEDWDVQACGGTHLPSTG 726
Query: 128 EIGKIKI 134
+G IKI
Sbjct: 727 LVGPIKI 733
>sp|Q57984|SYA_METJA ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE) (ALARS)
>gi|2118343|pir||D64370 alanine--tRNA ligase (EC
6.1.1.7) - Methanococcus jannaschii
>gi|1591270|gb|AAB98556.1| (U67505) alanyl-tRNA
synthetase (alaS) [Methanococcus jannaschii]
Length = 892
Score = 84.3 bits (205), Expect = 7e-16
Identities = 53/134 (39%), Positives = 76/134 (56%), Gaps = 8/134 (5%)
Query: 8 VRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEK 67
+R H+A H++ A KVLG R W + V ++ L + ++ S EE++ IE +ANE
Sbjct: 587 MRNHTATHIINAAAQKVLG-RHVWQAGSDVDVDKARLDITHYKRISREELKDIERVANEI 645
Query: 68 VRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWN-----VNACNKEH 122
V N IK + R EAE++FG +Y VP +V L++V+IED N V AC H
Sbjct: 646 VLNNYNIKSIFMDRNEAEEKFGFRIYQGGVVPGNV--LRIVIIEDENGNIVDVQACGGTH 703
Query: 123 VKRTGEIGKIKIRK 136
+ TGE+G IKI K
Sbjct: 704 CQNTGEVGFIKIIK 717
>gi|11499836 alanyl-tRNA synthetase (alaS) [Archaeoglobus fulgidus]
>gi|3334348|sp|O28029|SYA_ARCFU ALANYL-TRNA SYNTHETASE
(ALANINE--TRNA LIGASE) (ALARS) >gi|7437618|pir||G69531
alanyl-tRNA synthetase (alaS) homolog - Archaeoglobus
fulgidus >gi|2648270|gb|AAB89002.1| (AE000949)
alanyl-tRNA synthetase (alaS) [Archaeoglobus fulgidus]
Length = 906
Score = 84.3 bits (205), Expect = 7e-16
Identities = 56/130 (43%), Positives = 74/130 (56%), Gaps = 4/130 (3%)
Query: 8 VRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEK 67
+R HSA HV+ ++ KVLG W + ++ L V R+PSEEEI+ IE LAN +
Sbjct: 597 MRHHSATHVLLYSLQKVLGNHV-WQAGARKEFSKARLDVTHFRRPSEEEIKEIEMLANRE 655
Query: 68 VRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTG 127
+ N PIK + R EAE++FG +Y VP KI V V +D V AC H + TG
Sbjct: 656 ILANKPIKWEWMDRIEAERKFGFRLYQ-GGVPPGRKIRVVQVGDD--VQACGGTHCRSTG 712
Query: 128 EIGKIKIRKV 137
EIG +KI KV
Sbjct: 713 EIGMLKILKV 722
>sp|O58035|SYA_PYRHO ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE) (ALARS)
>gi|7437626|pir||C71455 alanine--tRNA ligase (EC
6.1.1.7) - Pyrococcus horikoshii
>gi|3256687|dbj|BAA29370.1| (AP000001) 915aa long
hypothetical alanyl-tRNA synthetase [Pyrococcus
horikoshii]
Length = 915
Score = 83.1 bits (202), Expect = 2e-15
Identities = 49/127 (38%), Positives = 74/127 (57%), Gaps = 3/127 (2%)
Query: 8 VRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEK 67
+R H+ HV+ GA+V+VLG R W + + + L + ++ SEEE++ IE LAN
Sbjct: 610 MRHHTGTHVLMGALVRVLG-RHVWQAGSQLTTDWARLDISHYKRISEEELKEIEMLANRI 668
Query: 68 VRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTG 127
V E+ + LPR AE+++G +Y VP + ++VV IEDW+V AC H+ TG
Sbjct: 669 VMEDRKVTWEWLPRTTAEQKYGFRLYQGGVVPG--REIRVVKIEDWDVQACGGTHLPSTG 726
Query: 128 EIGKIKI 134
+G IKI
Sbjct: 727 LVGPIKI 733
>gb|AAK40671.1| Alanyl-tRNA synthetase (alaS) [Sulfolobus solfataricus]
Length = 900
Score = 81.1 bits (197), Expect = 6e-15
Identities = 46/143 (32%), Positives = 76/143 (52%), Gaps = 4/143 (2%)
Query: 8 VRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEK 67
+R H+ HV+ A KVLG+ W +G L + + +EEE++ IE AN
Sbjct: 601 MRHHTVTHVILAAAKKVLGDHI-WQAGAEKTPEKGRLDITHHKALTEEEVKLIENYANSV 659
Query: 68 VRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTG 127
+ + P+K E+ R EAE ++G +Y+ VP I +++ I+DW++ +C HV T
Sbjct: 660 ISDRRPVKPLEMNRMEAEMKYGVSIYE-GGVPNSATI-RLLEIKDWDIESCGGTHVSNTS 717
Query: 128 EIGKIKIRKVRFRKSKGLLEVHF 150
EIG +KI V R G++ + +
Sbjct: 718 EIGAVKIINVE-RIQDGIIRLEY 739
>gb|AAG23137.1|AF245445_1 (AF245445) alanyl-tRNA synthetase [Giardia intestinalis]
Length = 954
Score = 77.6 bits (188), Expect = 7e-14
Identities = 50/140 (35%), Positives = 72/140 (50%), Gaps = 4/140 (2%)
Query: 11 HSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEKVRE 70
H+A HV+ KVLG W L + R + +E R IE AN +R+
Sbjct: 631 HTAAHVIHATANKVLGPHV-WQAGAKKTTEVATLDITHYRSLTFDEERAIEREANRMIRD 689
Query: 71 NIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTGEIG 130
NIPI+ Y+LP+ EAEK++G +Y VP ++ V I D + AC H+ TG+IG
Sbjct: 690 NIPIRKYQLPKNEAEKKYGFSLYQGGVVPGST--VRCVDITDHDTEACCGTHLDNTGKIG 747
Query: 131 KIKIRKVRFRKSKGLLEVHF 150
I+I K R S G++ + F
Sbjct: 748 LIRIVK-STRISDGVVRLTF 766
>gb|AAF05594.1|AF188719_1 (AF188719) alanyl-tRNA synthetase [Giardia intestinalis]
Length = 969
Score = 76.1 bits (184), Expect = 2e-13
Identities = 49/140 (35%), Positives = 72/140 (51%), Gaps = 4/140 (2%)
Query: 11 HSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEKVRE 70
H+A HV+ KVLG W L + R + +E R IE AN +R+
Sbjct: 646 HTAAHVIHATANKVLGPHV-WQAGAKKTTEVATLDITHYRSLTFDEERAIEREANRMIRD 704
Query: 71 NIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTGEIG 130
NIPI+ Y+LP+ +AEK++G +Y VP ++ V I D + AC H+ TG+IG
Sbjct: 705 NIPIRKYQLPKNKAEKKYGFSLYQGGVVPGST--VRCVDITDHDTEACCGTHLDNTGKIG 762
Query: 131 KIKIRKVRFRKSKGLLEVHF 150
I+I K R S G++ + F
Sbjct: 763 LIRIVK-STRISDGVVRLTF 781
>sp|O27718|SYA_METTH ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE) (ALARS)
>gi|7437627|pir||A69092 alanine--tRNA ligase (EC
6.1.1.7) - Methanobacterium thermoautotrophicum (strain
Delta H) >gi|2622813|gb|AAB86155.1| (AE000926)
alanyl-tRNA synthetase [Methanobacterium
thermoautotrophicum]
Length = 898
Score = 75.7 bits (183), Expect = 3e-13
Identities = 50/142 (35%), Positives = 75/142 (52%), Gaps = 4/142 (2%)
Query: 9 RTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEKV 68
R H+A H++ A KVLG+ W L + R+ + EE++ IE LANE V
Sbjct: 587 RNHTATHLIVSAARKVLGDHI-WQAGAQKGVKSSRLDLSHYRRITLEELQEIERLANEYV 645
Query: 69 RENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTGE 128
N+P+KV + R+ AE+++G +Y VP ++VV I +V AC HV RTG+
Sbjct: 646 MMNVPLKVRWMDRDLAERKYGFILYQGGVVPG--SRIRVVEIPGVDVQACAGTHVHRTGD 703
Query: 129 IGKIKIRKVRFRKSKGLLEVHF 150
IG I+I + R G+ + F
Sbjct: 704 IGLIRINRTE-RIQDGVERIEF 724
>sp|Q9Y9X3|SYA_AERPE ALANYL-TRNA SYNTHETASE (ALANINE--TRNA LIGASE) (ALARS)
>gi|7437623|pir||A72524 probable alanyl-tRNA synthetase
APE2166 - Aeropyrum pernix (strain K1)
>gi|5105865|dbj|BAA81177.1| (AP000063) 900aa long
hypothetical alanyl-tRNA synthetase [Aeropyrum pernix]
Length = 900
Score = 73.4 bits (177), Expect = 1e-12
Identities = 43/127 (33%), Positives = 70/127 (54%), Gaps = 3/127 (2%)
Query: 8 VRTHSALHVVKGAVVKVLGERAKWTYSTYVKGNRGVLTVKFDRKPSEEEIRRIEELANEK 67
+R H+ HV+ A +VLG A W +G L + R + ++I ++E + N+
Sbjct: 584 MRHHTVTHVLIAAARRVLGRHA-WQAGAEKTEEKGRLDITHHRPLTRDDIEKLENVVNQV 642
Query: 68 VRENIPIKVYELPREEAEKRFGEEMYDLFPVPEDVKILKVVVIEDWNVNACNKEHVKRTG 127
+RE + + + EAE+++G +Y VP + K L++V +EDW+V AC HV+ TG
Sbjct: 643 IRERRRVWEDMVDKNEAEEKYGFTIYQ-GGVPME-KRLRLVFVEDWDVEACFGTHVRNTG 700
Query: 128 EIGKIKI 134
EIG KI
Sbjct: 701 EIGGFKI 707
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.137 0.390
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58366810
Number of Sequences: 2977
Number of extensions: 2420838
Number of successful extensions: 7613
Number of sequences better than 1.0e-10: 12
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 7587
Number of HSP's gapped (non-prelim): 16
length of query: 159
length of database: 189,106,746
effective HSP length: 55
effective length of query: 104
effective length of database: 156,189,961
effective search space: 16243755944
effective search space used: 16243755944
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 162 (67.5 bits)