BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1443 (PAB1443) DE:SIMILARITY TO MYXOCOCCUS XANTHUS GUFA PROTEIN (254 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75052 gufA protein homolog PAB1443 - Pyrococcus abyssi (st... 495 e-139 pir||H71107 gufA protein homolog - Pyrococcus horikoshii >gi|325... 352 3e-96 pir||D70456 gufA protein homolog aq_1814 - Aquifex aeolicus >gi|... 210 2e-53 pir||G72550 probable gufA protein APE1691 - Aeropyrum pernix (st... 208 4e-53 pir||A72218 gufA protein - Thermotoga maritima (strain MSB8) >gi... 169 4e-41 pir||C83088 hypothetical protein PA4467 [imported] - Pseudomonas... 153 1e-36 pir||A69162 gufA protein homolog MTH473 - Methanobacterium therm... 147 1e-34 pir||A75447 gufA protein - Deinococcus radiodurans (strain R1) >... 137 2e-31 sp|Q06916|GUFA_MYXXA GUFA PROTEIN >gi|628449|pir||S39876 gufA pr... 133 2e-30 pir||C70127 gufA protein homolog - Lyme disease spirochete >gi|2... 133 2e-30 pir||T29905 hypothetical protein F59A3.4 - Caenorhabditis elegan... 115 4e-25 pir||F81444 probable integral membrane protein Cj0263 [imported]... 98 1e-19 pir||G81230 conserved hypothetical protein NMB0175 [imported] - ... 90 3e-17 gb|AAG19368.1| (AE005031) GufA protein; GufA [Halobacterium sp. ... 89 4e-17 pir||D82001 probable integral membrane protein NMA0093 [imported... 89 5e-17 sp|P24198|YGIE_ECOLI HYPOTHETICAL 26.5 KD PROTEIN IN TOLC-RIBB I... 81 2e-14 gb|AAA69208.1| (U28377) ORF_o265; alternate name ygiE; orfB of M... 81 2e-14 dbj|BAB02495.1| (AB025629) gene_id:MOE17.18~unknown protein [Ara... 75 1e-12 gb|AAF26056.1|AC012562_11 (AC012562) unknown protein; 37802-3561... 74 2e-12 >pir||E75052 gufA protein homolog PAB1443 - Pyrococcus abyssi (strain Orsay) >gi|5458827|emb|CAB50314.1| (AJ248287) SIMILARITY TO MYXOCOCCUS XANTHUS GUFA PROTEIN [Pyrococcus abyssi] Length = 254 Score = 495 bits (1261), Expect = e-139 Identities = 254/254 (100%), Positives = 254/254 (100%) Query: 1 MNETLILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPA 60 MNETLILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPA Sbjct: 1 MNETLILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPA 60 Query: 61 IDISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIH 120 IDISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIH Sbjct: 61 IDISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIH 120 Query: 121 NLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGV 180 NLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGV Sbjct: 121 NLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGV 180 Query: 181 AEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFF 240 AEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFF Sbjct: 181 AEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFF 240 Query: 241 LGFYLMLFLDTALG 254 LGFYLMLFLDTALG Sbjct: 241 LGFYLMLFLDTALG 254 >pir||H71107 gufA protein homolog - Pyrococcus horikoshii >gi|3257039|dbj|BAA29722.1| (AP000003) 269aa long hypothetical Gufa protein [Pyrococcus horikoshii] Length = 269 Score = 352 bits (893), Expect = 3e-96 Identities = 163/250 (65%), Positives = 208/250 (83%), Gaps = 2/250 (0%) Query: 5 LILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDIS 64 +I+A G+FVA MT+LG+L+A+F++RMP WG+D SL+FAAGVMIVASFTSLILPAID++ Sbjct: 22 IIVAVYAGIFVALMTTLGALIAIFANRMPDWGMDVSLSFAAGVMIVASFTSLILPAIDLT 81 Query: 65 SSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIHNLPE 124 SF P GIGI LG+ ++ +D FIPHEH+++GYEGPKEFK+K+K WL+ A+IIHNLPE Sbjct: 82 GSFTPAGIGIFLGVLLIYGIDNFIPHEHIIKGYEGPKEFKEKLKLVWLVVLAVIIHNLPE 141 Query: 125 GIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGVAEMI 184 G+A+G+S+ Y+ G++T +AIGIQD PEGT VSLPLAT+QKKRL+PILLG LSG+AEM+ Sbjct: 142 GLAVGISIVYNLNAGLVTAIAIGIQDFPEGTVVSLPLATIQKKRLVPILLGALSGIAEMV 201 Query: 185 MVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFFLGFY 244 MV+LG FS LPYGL +AGGAMLYVT+KEMIPEIY ++ NE ++T GFFLGFY Sbjct: 202 MVILGAFLFSIFKSFLPYGLGLAGGAMLYVTVKEMIPEIYK--RQENEKLVTLGFFLGFY 259 Query: 245 LMLFLDTALG 254 +MLFLD+ LG Sbjct: 260 VMLFLDSMLG 269 >pir||D70456 gufA protein homolog aq_1814 - Aquifex aeolicus >gi|2984109|gb|AAC07647.1| (AE000757) hypothetical protein [Aquifex aeolicus] Length = 243 Score = 210 bits (528), Expect = 2e-53 Identities = 119/249 (47%), Positives = 155/249 (61%), Gaps = 11/249 (4%) Query: 3 ETLILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAID 62 E L++A L L T LGS L+LF S V SLAFAAGVM+VASFTSLILP I+ Sbjct: 2 EILLIASLLSLG----TFLGSFLSLFFR---SVNVGVSLAFAAGVMLVASFTSLILPGIE 54 Query: 63 ISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIHNL 122 I F+ GI LG F+++L++ PHEH+V+G EG + K+ + L+ + IHN+ Sbjct: 55 IGG-FWKTATGIVLGFFLMMLVEVLSPHEHVVKGKEGLIK-KESLNRLILIVIGITIHNV 112 Query: 123 PEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGVAE 182 PEGI++GV+ ++S G +AI +QD+PEG VSLPL + K L+P+++G LSG E Sbjct: 113 PEGISVGVATSHSWNTGFPLAIAIAVQDIPEGLVVSLPLMVMMKSTLIPLIIGFLSGFIE 172 Query: 183 MIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFFLG 242 I V G LLPYGL GGAMLYVT+KE +PEIY G+ E IT F G Sbjct: 173 SIFAVFGYYLMETFKNLLPYGLGFGGGAMLYVTVKEALPEIYASGE--RETKITLSFLTG 230 Query: 243 FYLMLFLDT 251 F LMLFLD+ Sbjct: 231 FLLMLFLDS 239 >pir||G72550 probable gufA protein APE1691 - Aeropyrum pernix (strain K1) >gi|5105379|dbj|BAA80692.1| (AP000062) 269aa long hypothetical gufA protein [Aeropyrum pernix] Length = 269 Score = 208 bits (525), Expect = 4e-53 Identities = 110/251 (43%), Positives = 161/251 (63%), Gaps = 9/251 (3%) Query: 8 AFKLGLFVAFMTSLGSLLALFSH----RMPSWGVDFSLAFAAGVMIVASFTSLILPAIDI 63 AF L L+ MTSLG L L + R+ +D + F++G+MIVASFTSL+LPA++I Sbjct: 24 AFLLSLYAFAMTSLGGFLVLAARTHGGRLDVL-LDVGMGFSSGIMIVASFTSLLLPALEI 82 Query: 64 SSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIHNLP 123 S + I +G + ++++ +PHEHL++GYEGP F+ K+KAAWL+A A+IIHNLP Sbjct: 83 SGPGIVI-ISFIVGAIAVYIINEILPHEHLIKGYEGPPSFRRKVKAAWLVATAIIIHNLP 141 Query: 124 EGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGVAEM 183 EG++IG + +Y+ ++G+ LAIG QD PEG AVSLP+ + +L+ +LSG +E+ Sbjct: 142 EGMSIGAAASYAISEGLAVALAIGTQDFPEGLAVSLPVFAASGSLYLALLVAMLSGFSEV 201 Query: 184 IMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFFLGF 243 + + +S LL L+ A GAM++V E +PE + G NE + T GFF GF Sbjct: 202 VAATIVAALIGYSPGLLASALAFAAGAMVFVVSYEALPESHRSG---NEKLATVGFFAGF 258 Query: 244 YLMLFLDTALG 254 +ML+LDTALG Sbjct: 259 IIMLYLDTALG 269 >pir||A72218 gufA protein - Thermotoga maritima (strain MSB8) >gi|4982315|gb|AAD36803.1|AE001812_13 (AE001812) gufA protein [Thermotoga maritima] Length = 245 Score = 169 bits (423), Expect = 4e-41 Identities = 101/238 (42%), Positives = 142/238 (59%), Gaps = 10/238 (4%) Query: 19 TSLGSLLALF--SHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISSSFFPVGIGIAL 76 TSLG+L LF H +D L FAAGVM+ AS SL+ P++++ IG L Sbjct: 16 TSLGALPFLFLKPHHTSDKVIDSFLGFAAGVMLAASAFSLVAPSLEMGG-IVRFLIGFVL 74 Query: 77 GIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIHNLPEGIAIGVSLAYSK 136 G + L DK IPHEHL++G+EGP ++K WL A+ IHN PEG+A+GVS A++ Sbjct: 75 GGLFVNLADKLIPHEHLLKGHEGPDT--KRLKGVWLFIIAITIHNFPEGMAVGVS-AFTP 131 Query: 137 TDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGVAEMIMVVLGVIFFSFS 196 + +AIG+Q++PEG AV L ++ K+ L+ L+G+ E I +LG S S Sbjct: 132 -QALAIAIAIGVQNIPEGAAVMASLIPMKYKKGKAFLITFLTGLVEAIGGLLGAGIVSIS 190 Query: 197 SKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFFLGFYLMLFLDTALG 254 +LLPY ++ A GAM+YV E+IPE + KG NEL+ T+ +GF +M LD ALG Sbjct: 191 QRLLPYMMAFAAGAMIYVVSDEVIPETHSKG---NELLSTWWIMVGFLVMASLDVALG 245 >pir||C83088 hypothetical protein PA4467 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9950703|gb|AAG07855.1|AE004860_11 (AE004860) hypothetical protein [Pseudomonas aeruginosa] Length = 310 Score = 153 bits (384), Expect = 1e-36 Identities = 98/258 (37%), Positives = 137/258 (52%), Gaps = 13/258 (5%) Query: 5 LILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDIS 64 ++LA K GL AF T+LG++ L MP+ D L F AGVM+ A+ SLI+PA+ + Sbjct: 58 IVLALKGGLLCAFGTALGAMPVLVVKTMPARLSDALLGFGAGVMLSATAFSLIMPALSAA 117 Query: 65 SSF--------FPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFA 116 F V G+A+G+ L +L + +P H G EG L A Sbjct: 118 GDLGYSKFGAGFLVSFGLAVGVMGLFILGRLMPDVH--PGCEGAPLSGGIPPRILLFVTA 175 Query: 117 MIIHNLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGV 176 +++HN+PEG+A+GVS + L I +QDVPEG V+L LA + R +L+G Sbjct: 176 IVLHNIPEGMAVGVSAGAGLDEANGLALGIALQDVPEGLVVALVLAGVGMSRFKAMLVGA 235 Query: 177 LSGVAEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIIT 236 SG+ E + VL S+ LLP+GL+ A GAML+V E+IPE + +G T Sbjct: 236 ASGLVEPLFAVLCAWLVGLSALLLPWGLAAAAGAMLFVVTHEIIPESHRQGHAAE---AT 292 Query: 237 FGFFLGFYLMLFLDTALG 254 G GF LM+ LDTALG Sbjct: 293 LGLVFGFCLMMVLDTALG 310 >pir||A69162 gufA protein homolog MTH473 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2621542|gb|AAB84979.1| (AE000831) conserved protein [Methanobacterium thermoautotrophicum] Length = 259 Score = 147 bits (368), Expect = 1e-34 Identities = 101/259 (38%), Positives = 138/259 (52%), Gaps = 23/259 (8%) Query: 8 AFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISSS- 66 AF +F MT G+ L + +D SL FAAG+MI ASF SL++PAI++S + Sbjct: 10 AFLATIFTWLMTLAGAALVFLPLQGDRKLLDSSLGFAAGIMIAASFWSLLVPAIELSPTG 69 Query: 67 ---FFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAW----LLAFAMII 119 + P G G L +D+ IPH H EG + W LL A++I Sbjct: 70 VYAWVPPAAGFLAGGLFLSAMDRIIPHLHPGCDPEG-------LPTTWRRNRLLLLAVVI 122 Query: 120 HNLPEGIAIGVSL-AYSKTDGIITGLA----IGIQDVPEGTAVSLPLATLQKKRLMPILL 174 HNLPEG+AIGV+ A + T G+ LA IGIQ++PEG AVSLPL R + Sbjct: 123 HNLPEGLAIGVAFGAAASTGGLAAPLALALGIGIQNLPEGAAVSLPLLGEGLSRWRSFIY 182 Query: 175 GVLSGVAEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELI 234 G LSGV E++ + G + S ++PY L A GAM++V I+++IPE G N + Sbjct: 183 GQLSGVIEVLGGIGGALMVYSFSGIMPYALGFAAGAMIFVVIEDLIPECQSGG---NTDL 239 Query: 235 ITFGFFLGFYLMLFLDTAL 253 T +GF LM+ LD AL Sbjct: 240 ATVSALMGFVLMMVLDLAL 258 >pir||A75447 gufA protein - Deinococcus radiodurans (strain R1) >gi|6458760|gb|AAF10605.1|AE001954_9 (AE001954) gufA protein [Deinococcus radiodurans] Length = 256 Score = 137 bits (341), Expect = 2e-31 Identities = 98/257 (38%), Positives = 131/257 (50%), Gaps = 36/257 (14%) Query: 13 LFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISSSFFPVGI 72 +F +T+LG+ LF+ + + ++ + AAGVM+ ASF SL+ PAI Sbjct: 21 IFTWSLTALGASAVLFTRSVSNRLLNIAQGLAAGVMLAASFWSLLAPAI----------- 69 Query: 73 GIALGIFILVLLDKFIPHEHLVRGY---EGPKEFKDKIKAAW----LLAFAMIIHNLPEG 125 G+ LG + LLDK +PH H + EGP A W LL AM +HN PEG Sbjct: 70 GLLLGAAFVGLLDKLLPHLHPGFSHGEAEGPP-------ARWSQGALLLAAMTLHNFPEG 122 Query: 126 IAIGVSL--AYSKTDGIITG------LAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVL 177 +A+GVS A + G G L IG+Q++PEG AV+LPL R + G Sbjct: 123 MAVGVSFGAAGAGAGGATLGGALALALGIGLQNIPEGLAVALPLRAAGLSRRRAFMFGQA 182 Query: 178 SGVAEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITF 237 S + E + LG F S L+PY LS A GAM++V I+E+IPE G N I T Sbjct: 183 SALVEPVGGWLGAAFVGTSLPLMPYALSFAAGAMIFVVIEELIPEAQRGG---NADIATQ 239 Query: 238 GFFLGFYLMLFLDTALG 254 LGF M+ LD ALG Sbjct: 240 ATLLGFVAMMILDVALG 256 >sp|Q06916|GUFA_MYXXA GUFA PROTEIN >gi|628449|pir||S39876 gufA protein - Myxococcus xanthus >gi|49253|emb|CAA50385.1| (X71062) orfX [Myxococcus xanthus] >gi|861060|emb|CAA50380.1| (X71062) gufA [Myxococcus xanthus] Length = 254 Score = 133 bits (331), Expect = 2e-30 Identities = 89/251 (35%), Positives = 125/251 (49%), Gaps = 14/251 (5%) Query: 13 LFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDI--------S 64 L T LG+L L + + + G SL++PA+++ Sbjct: 9 LLAGTATGLGALPVLVTSELSRKAQGPDVGLQRGRDAGGQSFSLVIPAMELVRGQGHDGP 68 Query: 65 SSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIHNLPE 124 S+ V G+ LG L + +PHEH ++G+EG K +A L AM +HN PE Sbjct: 69 SAALRVAAGVLLGGLFLRVWHDLMPHEHALKGHEG--HGGTKWNSALLFVLAMTLHNFPE 126 Query: 125 GIAIGVSLAYSKTD-GIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGVAEM 183 G+A+GVS A + + G+ L IG Q++PEG V+L L L +L+G+ E Sbjct: 127 GLAVGVSFAAPQPELGLSVALGIGAQNIPEGLVVALALRASGASASRAAFLALLTGMVEP 186 Query: 184 IMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFFLGF 243 + + GV+ S SS LLP+GL+ AGGAMLYV EMIPE + G E T G GF Sbjct: 187 VGALFGVLALSLSSALLPWGLAFAGGAMLYVISHEMIPESHRGGFERE---ATTGLMWGF 243 Query: 244 YLMLFLDTALG 254 L L LD +LG Sbjct: 244 VLALVLDMSLG 254 >pir||C70127 gufA protein homolog - Lyme disease spirochete >gi|2688111|gb|AAC66605.1| (AE001132) gufA protein [Borrelia burgdorferi] Length = 273 Score = 133 bits (331), Expect = 2e-30 Identities = 81/254 (31%), Positives = 140/254 (54%), Gaps = 17/254 (6%) Query: 14 FVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISS-----SFF 68 F F T+ G+ F ++ + +D L F+AG+MI ASF SLI PAI+ + ++ Sbjct: 24 FTWFTTAFGAAAVFFFRKVDNKIMDAMLGFSAGIMIAASFFSLIQPAIERAEELGYITWV 83 Query: 69 PVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWLLAFAMIIHNLPEGIAI 128 P G +G F + ++D F+P + + ++ K +LL A+ +HN PEG+A+ Sbjct: 84 PAVFGFLVGAFFIYIVDVFVPDLDKLTFID--EDLTKHGKKDFLLFTAVTLHNFPEGLAV 141 Query: 129 GVSLAYSKTD--------GIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGV 180 GV+ ++ ++ L IGIQ++PEG A+SLPL G +SG+ Sbjct: 142 GVAFGALASNPDIQTLVGAMLLTLGIGIQNIPEGAAISLPLRRGNVALAKCFNYGQMSGL 201 Query: 181 AEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFF 240 E++ ++G +++LP+ L+ + GAM+YV+I+++IPE K K+++ + + Sbjct: 202 VEIVGGLMGAYAVYSFTRILPFALAFSAGAMIYVSIEQLIPE--AKRKDIDNKVPSIFGV 259 Query: 241 LGFYLMLFLDTALG 254 +GF LM+FLD +LG Sbjct: 260 IGFTLMMFLDVSLG 273 >pir||T29905 hypothetical protein F59A3.4 - Caenorhabditis elegans >gi|1707057|gb|AAB37816.1| (U80448) coded for by C. elegans cDNA CEESS55F; coded for by C. elegans cDNA yk84a1.3; coded for by C. elegans cDNA yk78g7.3; coded for by C. elegans cDNA yk168g9.5; coded for by C. elegans cDNA yk78g7.5; coded for by C. elegans cDNA yk84a1.5; strong s> Length = 321 Score = 115 bits (286), Expect = 4e-25 Identities = 88/304 (28%), Positives = 128/304 (41%), Gaps = 70/304 (23%) Query: 18 MTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISSS------FFPV- 70 +T+LG+ L +D SL FAAGVM ASF SL+ PAI+I+ + F PV Sbjct: 21 VTALGASLVFIMRHQSKKLLDISLGFAAGVMTAASFWSLLAPAIEIAEADFEKWAFVPVA 80 Query: 71 -GIGIALGIFILV----------------------------------------LLDKFIP 89 G + G L L + + Sbjct: 81 IGFAVGAGFVHLADTLLPSCVGDAGMTSLLSPPTRRSETEMSLMQAREDLDVAALARSVN 140 Query: 90 HEHLVRGYE------------GPKEFKDKIKAAWLLAFAMIIHNLPEGIAIGVSLAYSKT 137 EH R ++++ + LL A+ +HN+PEG+A+GV + Sbjct: 141 EEHRERDRSVDMGSDKRPDVIPEEDYRQSWRRILLLILAVTVHNIPEGLAVGVGFGSAGK 200 Query: 138 DGIIT-------GLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGVAEMIMVVLGV 190 T + IG+Q+ PEG AVSLPLA +L G LSG+ E I + G Sbjct: 201 TKQATFESAFNLAIGIGLQNFPEGLAVSLPLAAFGHSKLKAFWYGQLSGMVEPIAALFGA 260 Query: 191 IFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFFLGFYLMLFLD 250 F +LPY L+ A GAM+YV + ++IPE G N + + G +GF +M+ +D Sbjct: 261 AAVIFMEPVLPYALAFAAGAMIYVVVDDIIPEAQRNG---NGKLASLGCIIGFLVMMCMD 317 Query: 251 TALG 254 LG Sbjct: 318 VGLG 321 >pir||F81444 probable integral membrane protein Cj0263 [imported] - Campylobacter jejuni (strain NCTC 11168) >gi|6967740|emb|CAB72731.1| (AL139074) putative integral membrane protein [Campylobacter jejuni] Length = 291 Score = 97.9 bits (240), Expect = 1e-19 Identities = 88/293 (30%), Positives = 135/293 (46%), Gaps = 49/293 (16%) Query: 3 ETLILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAI- 61 E + +A L LF F T++GS++A FS + + L F+AGVMI SF ++ A+ Sbjct: 6 EQIFIAMLLTLFAGFSTAIGSIIAFFSRKDDLRVLSLGLGFSAGVMIYISFMEILPTALK 65 Query: 62 ---DISSSFFPVGIGIAL---GIFILVLLDKFIPHEHLVRGYEGPKEFKDKIKAAWL--- 112 + S + +G+A GI I +L+DK IP + V +E PKE ++K L Sbjct: 66 DFKNHYDSHWAELLGLACFFGGILISLLIDKLIPED--VNPHE-PKEDLSELKICPLPQK 122 Query: 113 ---------------------------LAFAMIIHNLPEGIAIGVSLAYSKTDGIITGLA 145 A A+ IHN PEG A +S + T GI +A Sbjct: 123 GQNPPKFHPGEKLHQINTKALKRTGIFTALAIAIHNFPEGFATFISSLDNLTLGIAIAIA 182 Query: 146 IGIQDVPEGTAVSLPL--ATLQKKRLMPILLGVLSGVAEMIMVVLG-VIFFSFSSKL-LP 201 + I ++PEG AVSLP+ AT KK+ + LSG AE + +G +I F L L Sbjct: 183 VAIHNIPEGLAVSLPIYHATGDKKK--AFIYSALSGFAEPLGAFVGALILLPFIGDLTLA 240 Query: 202 YGLSMAGGAMLYVTIKEMIPEIYMKGKEVNELIITFGFFLGFYLMLFLDTALG 254 ++ G M+++++ E++P K + L +G G +M LG Sbjct: 241 ISFAVIAGIMVFISLDELLPAAKTYDKAHDSL---YGLIAGMAIMALSLNLLG 290 >pir||G81230 conserved hypothetical protein NMB0175 [imported] - Neisseria meningitidis (group B strain MD58) >gi|7225393|gb|AAF40632.1| (AE002374) conserved hypothetical protein [Neisseria meningitidis MC58] Length = 269 Score = 89.7 bits (219), Expect = 3e-17 Identities = 67/259 (25%), Positives = 115/259 (43%), Gaps = 15/259 (5%) Query: 5 LILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPA---- 60 L +AF + L T LGS L +FS + F LAFA G M+ S T + + Sbjct: 9 LAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFGLAFAGGAMVYVSLTEIFSKSSEAF 68 Query: 61 ---IDISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDK----IKAAWLL 113 D +F + G+ + L+D+ +P+ H + P + K + + Sbjct: 69 AEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNPHETLDAQDPSFQESKRRHIARVGMMA 128 Query: 114 AFAMIIHNLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPIL 173 AFA+ HN PEG+A + + G+ LAI I ++PEG +++ P+ + R + Sbjct: 129 AFAITAHNFPEGLATFFATLENPAVGMPLALAIAIHNIPEGISIAAPVYFATRSRKKTVW 188 Query: 174 LGVLSGVAEMIMVVLGVIFFS--FSSKLLPYGLSMAGGAMLYVTIKEMIP--EIYMKGKE 229 +LSG+AE + LG + S + + G M+++ + E++P + Y G E Sbjct: 189 ACLLSGLAEPLGAALGYLVLQPFLSPAVFGSVFGVIAGVMVFLALDELLPAAKRYSDGHE 248 Query: 230 VNELIITFGFFLGFYLMLF 248 + T + L+LF Sbjct: 249 TVYGLTTGMAVIAVSLVLF 267 >gb|AAG19368.1| (AE005031) GufA protein; GufA [Halobacterium sp. NRC-1] Length = 266 Score = 89.3 bits (218), Expect = 4e-17 Identities = 69/241 (28%), Positives = 113/241 (46%), Gaps = 35/241 (14%) Query: 3 ETLILAFKLGLFVAFMTSLGSLLALFSH------RMPSWGVDFSLAFAAGVMIVASFTSL 56 E L + F GL T LG+L F R+ WG+ A+G+M+ AS L Sbjct: 6 ELLFVVFA-GLLTDLATGLGALPFFFVDDIDDRWRVGLWGL------ASGIMVSASGFGL 58 Query: 57 ILPAIDISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYE-GPKEFKDK-IKAAWLLA 114 A++ + G G+ ++V+ EH++ +E P++ +K L+A Sbjct: 59 FREALNYGGPT-EIAAGALAGVALVVVA------EHVIDDHEFEPRDIAAADVKKLALIA 111 Query: 115 FAMIIHNLPEGIAIGVSLAYSKTDG-------------IITGLAIGIQDVPEGTAVSLPL 161 + +H+ PEG+A+GVS A DG + +AI I +VPEG AVS+PL Sbjct: 112 GVLTVHSFPEGVAVGVSFADMGIDGGLSVLGFSIPILAVFMTIAISIHNVPEGLAVSIPL 171 Query: 162 ATLQKKRLMPILLGVLSGVAEMIMVVLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIP 221 +R + + V + + + I + +F + LP+G AGGAM+++ + E IP Sbjct: 172 HEHGARRWTLVGVAVFTSIPQPIGAGIAYVFVQIARAALPFGYGFAGGAMVFLVLSEFIP 231 Query: 222 E 222 E Sbjct: 232 E 232 >pir||D82001 probable integral membrane protein NMA0093 [imported] - Neisseria meningitidis (group A strain Z2491) >gi|7378866|emb|CAB83409.1| (AL162752) putative integral membrane protein [Neisseria meningitidis Z2491] Length = 269 Score = 88.9 bits (217), Expect = 5e-17 Identities = 60/230 (26%), Positives = 104/230 (45%), Gaps = 13/230 (5%) Query: 5 LILAFKLGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPA---- 60 L +AF + L T LGS L +FS + F LAFA G M+ S T + + Sbjct: 9 LAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFGLAFAGGAMVYVSLTEIFSKSSEAF 68 Query: 61 ---IDISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYEGPKEFKDK----IKAAWLL 113 D +F + G+ + L+D+ +P+ H + P + K + + Sbjct: 69 AEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNPHETLDAQDPSFQESKRRHIARVGMMA 128 Query: 114 AFAMIIHNLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPIL 173 AFA+ HN PEG+A + + G+ LAI I ++PEG +++ P+ + R + Sbjct: 129 AFAITAHNFPEGLATFFATLENPAVGMPLALAIAIHNIPEGISIAAPVYFATRSRKKTVW 188 Query: 174 LGVLSGVAEMIMVVLGVIFFS--FSSKLLPYGLSMAGGAMLYVTIKEMIP 221 +LSG+AE + LG + S + + G M+++ + E++P Sbjct: 189 ACLLSGLAEPLGAALGYLVLQPFLSPAVFGSVFGVIAGVMVFLALDELLP 238 >sp|P24198|YGIE_ECOLI HYPOTHETICAL 26.5 KD PROTEIN IN TOLC-RIBB INTERGENIC REGION (ORFB) (O265) >gi|96076|pir||S22363 gufA protein homolog - Escherichia coli >gi|146680|gb|AAA71878.1| (M77129) ORFB [Escherichia coli] >gi|1789419|gb|AAC76076.1| (AE000386) orf, hypothetical protein [Escherichia coli K12] Length = 257 Score = 80.8 bits (196), Expect = 2e-14 Identities = 63/223 (28%), Positives = 111/223 (49%), Gaps = 19/223 (8%) Query: 11 LGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISSSFFPV 70 L + T +G+ L + + + + FSL FAAG+M++ S + LPA + PV Sbjct: 7 LTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIMLLISLMEM-LPAALAAEGMSPV 65 Query: 71 GIGIALGIFILVLL-----DKFIPHEH----LVRGYEG-PKEFKDKIKAAWLLAFAMIIH 120 + G+FI LL D+ +PH H + + + PK K + A LL + +H Sbjct: 66 ---LGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLPKSIK---RTAILLTLGISLH 119 Query: 121 NLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGV 180 N PEGIA V+ + + G LA+ + ++PEG AV+ P+ + IL +SG+ Sbjct: 120 NFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPVYAATGSKRTAILWAGISGL 179 Query: 181 AEMIMVVLG--VIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIP 221 AE++ VL ++ S ++ ++ G M+ +++ E++P Sbjct: 180 AEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVDELMP 222 >gb|AAA69208.1| (U28377) ORF_o265; alternate name ygiE; orfB of M77129 [Escherichia coli] Length = 265 Score = 80.8 bits (196), Expect = 2e-14 Identities = 63/223 (28%), Positives = 111/223 (49%), Gaps = 19/223 (8%) Query: 11 LGLFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISSSFFPV 70 L + T +G+ L + + + + FSL FAAG+M++ S + LPA + PV Sbjct: 15 LTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIMLLISLMEM-LPAALAAEGMSPV 73 Query: 71 GIGIALGIFILVLL-----DKFIPHEH----LVRGYEG-PKEFKDKIKAAWLLAFAMIIH 120 + G+FI LL D+ +PH H + + + PK K + A LL + +H Sbjct: 74 ---LGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLPKSIK---RTAILLTLGISLH 127 Query: 121 NLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGV 180 N PEGIA V+ + + G LA+ + ++PEG AV+ P+ + IL +SG+ Sbjct: 128 NFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPVYAATGSKRTAILWAGISGL 187 Query: 181 AEMIMVVLG--VIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIP 221 AE++ VL ++ S ++ ++ G M+ +++ E++P Sbjct: 188 AEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVDELMP 230 >dbj|BAB02495.1| (AB025629) gene_id:MOE17.18~unknown protein [Arabidopsis thaliana] Length = 374 Score = 74.5 bits (180), Expect = 1e-12 Identities = 61/223 (27%), Positives = 100/223 (44%), Gaps = 29/223 (13%) Query: 39 FSLAFAAGVMIVASFTSLILPAIDISSSFFPVGIGIALGIFILVLLDKFIPHEHLVRGYE 98 F FA+G+M+ SF L AI+ S FF + G E Sbjct: 145 FLQGFASGLMLSISFLDLAHNAIN-SIGFFKANLW--------------------KNGDE 183 Query: 99 GPKEFKDK-----IKAAWLLAFAMIIHNLPEGIAIGVSLAYSKTDGIITGLAIGIQDVPE 153 G K+ K + + + A + +HN PEG+A+ + G+ LAI + ++PE Sbjct: 184 GGKDMMKKHRKQVLYSGLITAIGISLHNFPEGMAVFLGSIKGMRVGVNLALAIALHNIPE 243 Query: 154 GTAVSLPLATLQKKRLMPILLGVLSGVAEMIMVVLGVIFF--SFSSKLLPYGLSMAGGAM 211 G AV+LP+ + + L LSG+AE + V++ F S S ++L L GG M Sbjct: 244 GVAVALPIYFATESKWQAFKLATLSGLAEPLGVIIVAYLFPRSLSPEILEGLLGAVGGIM 303 Query: 212 LYVTIKEMIPEIY-MKGKEVNELIITFGFFLGFYLMLFLDTAL 253 ++T+ EM+P + G++ + FG + FL+ +L Sbjct: 304 AFLTLHEMLPLAFDYAGQKQAVKAVFFGMACMSASLYFLELSL 346 >gb|AAF26056.1|AC012562_11 (AC012562) unknown protein; 37802-35617 [Arabidopsis thaliana] Length = 595 Score = 74.1 bits (179), Expect = 2e-12 Identities = 61/218 (27%), Positives = 107/218 (48%), Gaps = 16/218 (7%) Query: 13 LFVAFMTSLGSLLALFSHRMPSWGVDFSLAFAAGVMIVASFTSLILPAIDISSSFFPVGI 72 L +A T LG++ F P W AAGVM+ ASF + S ++ GI Sbjct: 83 LAMAAATGLGAVPFFFVELDPQWA-GICNGMAAGVMLAASFDLVKEGQEHGSGNWVVTGI 141 Query: 73 GIALGIFILV---LLDKFIPHEHL-VRGYEGPKEFKDKIKAAWLLAFAMIIHNLPEGIAI 128 +A +FI + +L+++ L ++G + K L+ M +H+ EG + Sbjct: 142 -LAGALFIWLCKQILEQYGEVSMLDIKGADATKVV--------LVIGIMTLHSFGEGSGV 192 Query: 129 GVSLAYSK--TDGIITGLAIGIQDVPEGTAVSLPLATLQKKRLMPILLGVLSGVAEMIMV 186 GVS A SK + G++ LAI + ++PEG AVS+ LA+ +L +++ + + ++ Sbjct: 193 GVSFAGSKGFSQGLLVTLAIAVHNIPEGLAVSMVLASRGVSPQNAMLWSIITSLPQPLVA 252 Query: 187 VLGVIFFSFSSKLLPYGLSMAGGAMLYVTIKEMIPEIY 224 V + SK LP+ A G M+++ I E++P+ + Sbjct: 253 VPAFLCADAFSKFLPFCTGFAAGCMIWMVIAEVLPDAF 290 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.329 0.146 0.423 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 85547738 Number of Sequences: 2977 Number of extensions: 3439053 Number of successful extensions: 12350 Number of sequences better than 1.0e-10: 19 Number of HSP's better than 0.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 12265 Number of HSP's gapped (non-prelim): 28 length of query: 254 length of database: 189,106,746 effective HSP length: 52 effective length of query: 202 effective length of database: 157,985,422 effective search space: 31913055244 effective search space used: 31913055244 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 40 (21.7 bits) S2: 164 (68.3 bits)