BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1449 (PAB1449) DE:hydrolase related
(262 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75051 hydrolase related PAB1449 - Pyrococcus abyssi (strai... 527 e-149
pir||C71109 hypothetical protein PH0642 - Pyrococcus horikoshii ... 474 e-133
emb|CAB45873.1| (Y19104) beta-alanine synthase [Lycopersicon esc... 145 5e-34
gb|AAD19716.1| (AF124349) hydrolase [Zymomonas mobilis] 136 4e-31
pir||F75263 probable hydrolase - Deinococcus radiodurans (strain... 136 4e-31
pir||B83387 hypothetical protein PA2074 [imported] - Pseudomonas... 134 1e-30
pir||G83608 probable hydratase PA0293 [imported] - Pseudomonas a... 130 2e-29
gi|9910460 Nit protein 2 [Homo sapiens] >gi|11433227|ref|XP_0030... 129 3e-29
gi|11497735 conserved hypothetical protein [Archaeoglobus fulgid... 129 3e-29
pir||B81369 probable hydrolase Cj0947c [imported] - Campylobacte... 129 5e-29
pir||T41662 probable nitrilase - fission yeast (Schizosaccharomy... 128 6e-29
pir||G71949 hypothetical protein jhp0294 - Helicobacter pylori (... 127 2e-28
pir||E64558 conserved hypothetical protein HP0309 - Helicobacter... 126 2e-28
gb|AAF87102.1|AF284573_1 (AF284573) Nit protein 2 [Mus musculus] 126 3e-28
pir||T28684 hypothetical protein - Streptomyces coelicolor >gi|3... 124 1e-27
pir||H82556 beta-alanine synthetase XF2443 [imported] - Xylella ... 124 1e-27
gi|9631647 contains ATP/GTP-binding site motif A; similar to rat... 122 6e-27
pir||T34905 probable hydrolase - Streptomyces coelicolor >gi|321... 119 3e-26
dbj|BAB04766.1| (AP001510) unknown conserved protein [Bacillus h... 118 7e-26
pir||H83195 conserved hypothetical protein PA3598 [imported] - P... 118 9e-26
gb|AAD15597.1| (AC006232) putative nitrilase [Arabidopsis thaliana] 116 2e-25
emb|CAB76877.1| (AL159139) putative hydrolase [Streptomyces coel... 115 4e-25
sp|P55176|YPQQ_PSEFL HYPOTHETICAL 31.2 KD PROTEIN IN PQQF 5'REGI... 114 1e-24
emb|CAA62550.1| (X91070) D-N-alpha-carbamilase [Agrobacterium ra... 110 2e-23
gb|AAB47607.1| (U59376) N-carbamoyl-D-amino acid amidohydrolase ... 109 3e-23
pir||JW0082 N-carbamyl-D-amino acid amidohydrolase (EC 3.5.-.-) ... 108 6e-23
pir||B69109 N-carbamoyl-D-amino acid amidohydrolase - Methanobac... 105 5e-22
emb|CAC12333.1| (AL445066) nitrilase related protein [Thermoplas... 105 6e-22
pir||E64614 beta-alanine synthetase homolog - Helicobacter pylor... 104 1e-21
pir||F71901 hypothetical protein jhp0694 - Helicobacter pylori (... 102 3e-21
pir||E69863 conserved hypothetical protein ykrU - Bacillus subti... 99 6e-20
pir||JW0083 N-carbamyl-D-amino acid amidohydrolase (EC 3.5.-.-) ... 98 1e-19
pir||B72408 conserved hypothetical protein - Thermotoga maritima... 96 3e-19
gb|AAF54370.1| (AE003682) CG8132 gene product [Drosophila melano... 96 3e-19
pir||T16068 hypothetical protein F13H8.7 - Caenorhabditis elegan... 95 9e-19
gi|7706509 beta-ureidopropionase [Homo sapiens] >gi|6288771|gb|A... 94 2e-18
gi|11417872 beta-ureidopropionase [Homo sapiens] 94 2e-18
pir||T48563 hypothetical protein F14F18.210 - Arabidopsis thalia... 94 2e-18
gi|6323383 nitrilase superfamily member; Nit3p [Saccharomyces ce... 93 3e-18
dbj|BAB09868.1| (AB008268) beta-ureidopropionase [Arabidopsis th... 93 4e-18
sp|Q03248|BUP_RAT BETA-UREIDOPROPIONASE (BETA-ALANINE SYNTHASE) ... 90 2e-17
gb|AAF06739.1| (AF169560) beta-ureidopropionase [Homo sapiens] 89 4e-17
pir||T36488 probable hydrolase - Streptomyces coelicolor >gi|545... 88 1e-16
gb|AAC39137.1| (AF069989) nitrilase and fragile histidine triad ... 87 2e-16
gb|AAF54141.1| (AE003676) CG3027 gene product [Drosophila melano... 87 2e-16
sp|Q9X0Y0|NAE2_THEMA PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHET... 87 3e-16
gb|AAC38297.1| (AF012132) unknown [Staphylococcus epidermidis] 84 2e-15
gb|AAF87104.1|AF284575_1 (AF284575) Nit protein 1 [Xenopus laevis] 83 3e-15
sp|Q10166|YAUB_SCHPO HYPOTHETICAL 35.7 KD PROTEIN C26A3.11 IN CH... 83 4e-15
gb|AAK40748.1| Conserved hypothetical protein [Sulfolobus solfat... 82 9e-15
pir||A72510 hypothetical protein APE2055 - Aeropyrum pernix (str... 81 1e-14
sp|P54608|YHCX_BACSU HYPOTHETICAL 60.2 KD PROTEIN IN CSPB-GLPP I... 81 2e-14
gi|6754856 nitrilase 1 [Mus musculus] >gi|3228668|gb|AAC40185.1|... 81 2e-14
gb|AAC40184.1| (AF069985) nitrilase homolog 1 [Mus musculus] 81 2e-14
pir||A70310 conserved hypothetical protein aq_103 - Aquifex aeol... 80 2e-14
sp|P46010|NRL3_ARATH NITRILASE 3 >gi|11266291|pir||T49148 nitril... 79 5e-14
sp|P32961|NRL1_ARATH NITRILASE 1 >gi|99738|pir||S22398 nitrilase... 79 6e-14
pir||T49147 nitrilase 1 - Arabidopsis thaliana >gi|1389699|gb|AA... 79 6e-14
sp|P55178|YAG5_STALU HYPOTHETICAL PROTEIN IN AGR OPERON (ORF 5) ... 79 6e-14
emb|CAA72932.1| (Y12252) AmiE protein, aliphatic amidase [Helico... 78 1e-13
pir||B71951 aliphatic amidase - Helicobacter pylori (strain J99)... 78 1e-13
gi|11499237 conserved hypothetical protein [Archaeoglobus fulgid... 78 1e-13
gb|AAF14257.1| (AF136599) aliphatic amidase [Bacillus stearother... 77 2e-13
pir||T27679 probable nitrilase (EC 3.5.5.1) ZK1058.6 - Caenorhab... 77 2e-13
pir||F64556 aliphatic amidase - Helicobacter pylori (strain 2669... 76 3e-13
sp|P32962|NRL2_ARATH NITRILASE 2 >gi|322548|pir||S31969 nitrilas... 76 4e-13
emb|CAA68934.3| (Y07648) nitrilase 2 [Arabidopsis thaliana] 76 4e-13
gb|AAB05220.1| (U38845) nitrilase 2 [Arabidopsis thaliana] 76 5e-13
dbj|BAA32602.1| (AB017194) hypothetical protein [Plectonema bory... 76 5e-13
sp|P55177|YAG5_STAAU HYPOTHETICAL 29.8 KD PROTEIN IN AGR OPERON ... 75 7e-13
dbj|BAA77679.1| (AB027054) nitrilase-like protein [Oryza sativa] 75 7e-13
pir||H83170 conserved hypothetical protein PA3797 [imported] - P... 75 1e-12
sp|P55175|Y601_SYNY3 HYPOTHETICAL 30.2 KD PROTEIN SLL0601 >gi|74... 73 3e-12
pir||T43198 nitrilase/Fhit protein - Caenorhabditis elegans >gi|... 73 5e-12
dbj|BAB04808.1| (AP001510) unknown conserved protein [Bacillus h... 73 5e-12
gb|AAF69000.1|AF257487_1 (AF257487) amidase [Bacillus sp. BR449] 71 1e-11
gb|AAK42348.1| Amidohydrolase, putative [Sulfolobus solfataricus] 71 1e-11
sp|Q01360|ALAM_RHOER ALIPHATIC AMIDASE (WIDE SPECTRUM AMIDASE) >... 71 1e-11
sp|Q03217|NRL2_RHORH ALIPHATIC NITRILASE >gi|322251|pir||A45070 ... 71 1e-11
sp|Q02068|NRL1_RHORH ALIPHATIC NITRILASE >gi|322250|pir||A43470 ... 71 2e-11
dbj|BAA90460.1| (AB028892) nitrilase [Bacillus sp. OxB-1] 71 2e-11
sp|P46011|NRL4_ARATH NITRILASE 4 >gi|508737|gb|AAA19628.1| (U099... 70 2e-11
gi|6322522 52-kDa amidase specific for N-terminal asparagine and... 70 2e-11
pir||B81199 nitrilase NMB0441 [imported] - Neisseria meningitidi... 70 3e-11
sp|Q42965|NRL4_TOBAC NITRILASE 4 >gi|7435979|pir||T03736 nitrila... 69 4e-11
pir||A72454 probable nitrilase APE2277 - Aeropyrum pernix (strai... 69 4e-11
gi|9055236 VNN3 protein [Homo sapiens] >gi|7160973|emb|CAB76824.... 69 4e-11
gi|11418681 VNN3 protein [Homo sapiens] 69 4e-11
pir||T03739 nitrilase (EC 3.5.5.1) 4B - common tobacco >gi|11816... 68 9e-11
pir||G75274 nitrilase-related protein - Deinococcus radiodurans ... 68 9e-11
>pir||C75051 hydrolase related PAB1449 - Pyrococcus abyssi (strain Orsay)
>gi|5458817|emb|CAB50304.1| (AJ248287) hydrolase related
[Pyrococcus abyssi]
Length = 262
Score = 527 bits (1344), Expect = e-149
Identities = 262/262 (100%), Positives = 262/262 (100%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA
Sbjct: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
Query: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYRE 120
QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYRE
Sbjct: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYRE 120
Query: 121 KFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRA 180
KFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRA
Sbjct: 121 KFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRA 180
Query: 181 MPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLV 240
MPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLV
Sbjct: 181 MPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLV 240
Query: 241 RNKRINDLNDIFKDRREEYYFR 262
RNKRINDLNDIFKDRREEYYFR
Sbjct: 241 RNKRINDLNDIFKDRREEYYFR 262
>pir||C71109 hypothetical protein PH0642 - Pyrococcus horikoshii
>gi|3257050|dbj|BAA29733.1| (AP000003) 262aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 262
Score = 474 bits (1208), Expect = e-133
Identities = 227/262 (86%), Positives = 251/262 (95%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
MVKV Y+QM P+ILE DKNYSKAEKLIKEASK+GA+LVVLPELFDTGYNFE+REEVF++A
Sbjct: 1 MVKVGYIQMEPKILELDKNYSKAEKLIKEASKEGAKLVVLPELFDTGYNFESREEVFDVA 60
Query: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYRE 120
Q+IPEGETTTFLM++AR+ G+YIVAGTAEK G+ LYNSAVVVGPRG+IGKYRKIHLFYRE
Sbjct: 61 QQIPEGETTTFLMELARELGLYIVAGTAEKSGNYLYNSAVVVGPRGYIGKYRKIHLFYRE 120
Query: 121 KFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRA 180
K FFEPGDLGF+VFD+GF KVGVMICFDWFFPESARTLALKGA++IAHPANLVMPYAPRA
Sbjct: 121 KVFFEPGDLGFKVFDIGFAKVGVMICFDWFFPESARTLALKGAEIIAHPANLVMPYAPRA 180
Query: 181 MPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLV 240
MPIRALEN+VYT+TADRVGEERGLKFIGKSLIASPKAEVLS+ASETEEE+GV EIDL L
Sbjct: 181 MPIRALENRVYTITADRVGEERGLKFIGKSLIASPKAEVLSIASETEEEIGVVEIDLNLA 240
Query: 241 RNKRINDLNDIFKDRREEYYFR 262
RNKR+ND+NDIFKDRREEYYFR
Sbjct: 241 RNKRLNDMNDIFKDRREEYYFR 262
>emb|CAB45873.1| (Y19104) beta-alanine synthase [Lycopersicon esculentum]
Length = 300
Score = 145 bits (362), Expect = 5e-34
Identities = 91/267 (34%), Positives = 148/267 (55%), Gaps = 28/267 (10%)
Query: 19 NYSKAEKLIKEASKQGAQLVVLPELFDTGYNF--ETREEVFEIAQKIPEGETTTFLMDVA 76
N + AE+L++ A ++GA ++++ ELF+ GY F +EE F A+ P T + ++A
Sbjct: 24 NVATAERLVRAAHQKGANIILIQELFE-GYYFCQAQKEEFFHRAKPYPGHPTIVRMQNLA 82
Query: 77 RDTGVYIVAGTAEKDGDVLYNSAVVVGPRGF-IGKYRKIHL----FYREKFFFEPGDLGF 131
++ GV I E+ + YNS ++ G +G YRK H+ Y+EK++F PGD GF
Sbjct: 83 KELGVVIPVSFFEEANNAHYNSVAIIDADGTDLGLYRKSHIPDGPGYQEKYYFNPGDTGF 142
Query: 132 RVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANL----------VMPYAPRAM 181
+VF + K+GV IC+D +FPE+AR +AL+GA+V+ +P + + R M
Sbjct: 143 KVFQTKYAKIGVAICWDQWFPEAARAMALQGAEVLFYPTAIGSEPQDDGLDSRDHWRRVM 202
Query: 182 PIRALENKVYTVTADRVGEE--------RGLKFIGKSLIASPKAEVLSMASETEEEVGVA 233
A N V V ++R+G+E + F G S IA P E+++ A + EE V VA
Sbjct: 203 QGHAGANVVPLVASNRIGKEIIETEHGNSEITFYGYSFIAGPTGELVAAAGDKEEAVLVA 262
Query: 234 EIDLYLVRNKRINDLNDIFKDRREEYY 260
+ DL +++KR +++DRR + Y
Sbjct: 263 QFDLDKIKSKRHG--WGVYRDRRPDLY 287
>gb|AAD19716.1| (AF124349) hydrolase [Zymomonas mobilis]
Length = 282
Score = 136 bits (338), Expect = 4e-31
Identities = 87/260 (33%), Positives = 137/260 (52%), Gaps = 18/260 (6%)
Query: 19 NYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREE-VFEIAQKIPEGETTTFLMDVAR 77
N + L++EA+ QGA++++ PELF+ Y T +E F A+ + + + +A+
Sbjct: 20 NIDRVSVLVEEAAAQGAKIILPPELFEGHYFCATEDESCFARAKPVEKHPVVLAMQKLAK 79
Query: 78 DTGVYIVAGTAEKDGDVLYNSAVVVGPRGFI-GKYRKIHL----FYREKFFFEPGDLGFR 132
V I E D YNS ++ G + G YRK H+ Y EKF+F PG+ GF+
Sbjct: 80 RLKVTIPTSFFESDPPHYYNSLAMINSDGEVMGVYRKSHIPDGPGYEEKFYFRPGNSGFK 139
Query: 133 VFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANL-VMPYAP---------RAMP 182
V+D G +K+GV IC+D ++PE+AR + L GA+++ P + P+ P RAM
Sbjct: 140 VWDCGGIKIGVGICWDQWYPETARAMMLMGAELLFFPTAIGNEPHDPDLDTSRLWRRAMI 199
Query: 183 IRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRN 242
A+ N V + ++R+G+E L F G S IA + +++ + E V VA D+ +R
Sbjct: 200 GHAVSNVVPVIASNRIGQEATLSFYGHSFIADQRGDLVQAFGKDESGVLVAHFDIEQIRQ 259
Query: 243 KRINDLNDIFKDRREEYYFR 262
R F+DRR E Y R
Sbjct: 260 HRA--AFGFFRDRRPELYHR 277
>pir||F75263 probable hydrolase - Deinococcus radiodurans (strain R1)
>gi|6460353|gb|AAF12070.1|AE002082_6 (AE002082)
hydrolase, putative [Deinococcus radiodurans]
Length = 297
Score = 136 bits (338), Expect = 4e-31
Identities = 93/280 (33%), Positives = 151/280 (53%), Gaps = 24/280 (8%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFET-REEVFEIA 60
V +A VQM+ + + N +A + ++EA+++GAQ+++LPELF+ Y + RE+ F +A
Sbjct: 8 VHLAVVQMH-MTDQLEDNVERAAEHVREAARRGAQVILLPELFENLYFCQVEREDYFGLA 66
Query: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRG-FIGKYRKIHL--- 116
+ ++AR+ V + EK G YNS V + G +G YRK H+
Sbjct: 67 HPLEGHPFIGRFQELARELNVVLPVSYFEKAGQAHYNSLVCIDAGGELLGNYRKTHIPDG 126
Query: 117 -FYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMP 175
Y EK++F PGD GF+V+D F +VGV IC+D ++PE+AR + L+GAD + +P +
Sbjct: 127 PGYEEKYYFNPGDTGFKVWDTRFGRVGVGICWDQWYPETARVMMLQGADFLLYPTAIGSE 186
Query: 176 YA-----------PRAMPIRALENKVYTVTADRVGEE--RGLK--FIGKSLIASPKAEVL 220
A RAM A+ N Y +++R+G+E GL+ + G S I+ E++
Sbjct: 187 PAEVETPNNHQMWQRAMVGHAVSNSSYVGSSNRIGKEIVGGLEQTYYGHSFISDYTGELV 246
Query: 221 SMASETEEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
+ ++EE + E++L R R F+DRR E Y
Sbjct: 247 AELGDSEEGPLLHELNLKEARKFRAG--MGFFRDRRPELY 284
>pir||B83387 hypothetical protein PA2074 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9948084|gb|AAG05462.1|AE004634_5
(AE004634) hypothetical protein [Pseudomonas aeruginosa]
Length = 270
Score = 134 bits (334), Expect = 1e-30
Identities = 93/270 (34%), Positives = 136/270 (49%), Gaps = 14/270 (5%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
V VA Q+ P+I + N AE+ I+EA+++GA +VVLPEL +GY F R E +A+
Sbjct: 4 VTVACCQIAPRIGAQEHNLRLAERAIREAARRGANVVVLPELAASGYVFADRGEALALAE 63
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEK-DGDVLYNSAVVVGPRGFIGKYRKIHLFYRE 120
+G + +A + + IV G E+ D + NSA +V G YRK HL+ E
Sbjct: 64 T-RDGPSLGLWKALAGELDLVIVGGFCERLDPQRVANSAALVDADGVRAIYRKAHLWNEE 122
Query: 121 KFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRA 180
FE G+ V F ++ VM+C+D FPE R AL GA ++ P N P APR
Sbjct: 123 SGIFEAGEQPPPVVATRFGRIAVMVCYDLEFPEWVRLPALSGAQLLCAPVN--WPAAPRP 180
Query: 181 MPIRALE----------NKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEV 230
R E N+++ DR G+ERG+ ++G S+I L + E +
Sbjct: 181 EGERPAEVVRVQANASVNRLFIAACDRCGQERGVDWVGGSVIVDADGYPLDGPLQGEAGM 240
Query: 231 GVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
+A +DL KRI+ N + +DRR E Y
Sbjct: 241 LLARLDLAEADEKRISAHNHVHRDRRPELY 270
>pir||G83608 probable hydratase PA0293 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9946137|gb|AAG03682.1|AE004467_4
(AE004467) probable hydratase [Pseudomonas aeruginosa]
Length = 292
Score = 130 bits (324), Expect = 2e-29
Identities = 89/279 (31%), Positives = 143/279 (50%), Gaps = 23/279 (8%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFET-REEVFEIA 60
V VA QM P N ++AEKL+++A+ +GAQ++++ ELF+T Y + + ++A
Sbjct: 5 VTVAATQMACSWDRP-ANIARAEKLVRQAAARGAQIILIQELFETPYFCQKPNPDYLQLA 63
Query: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNS-AVVVGPRGFIGKYRKIHL--- 116
+ E +AR+ V + E+ G +NS AV+ G +G YRK H+
Sbjct: 64 TTVEENAAIAHFQALARELQVVLPISFFERAGRARFNSIAVIDADGGNLGVYRKSHIPDG 123
Query: 117 -FYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANL--- 172
Y EK++F PGD GF+V+ + ++GV IC+D +FPESAR++AL GA+++ +P +
Sbjct: 124 PGYHEKYYFNPGDTGFKVWQTRYARIGVGICWDQWFPESARSMALLGAELLFYPTAIGSE 183
Query: 173 -------VMPYAPRAMPIRALENKVYTVTADRVGEER----GLKFIGKSLIASPKAEVLS 221
+ R A N + V ++R+G E + F G S IA P E +
Sbjct: 184 PHDASISSRDHWQRVQQGHAGANLMPLVASNRIGREEQDGYDITFYGSSFIADPFGEKVE 243
Query: 222 MASETEEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
+ TEE + V DL + +R +F+DRR Y
Sbjct: 244 ELNRTEEGILVHTFDLDAL--ERTRSAWGVFRDRRPNLY 280
>gi|9910460 Nit protein 2 [Homo sapiens] >gi|11433227|ref|XP_003017.1| Nit
protein 2 [Homo sapiens]
>gi|9367116|gb|AAF87103.1|AF284574_1 (AF284574) Nit
protein 2 [Homo sapiens]
Length = 276
Score = 129 bits (322), Expect = 3e-29
Identities = 90/274 (32%), Positives = 147/274 (52%), Gaps = 25/274 (9%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQK 62
++A +Q+ ++ D N ++A I+EA+ QGA++V LPE F++ Y + E A+K
Sbjct: 5 RLALIQLQISSIKSD-NVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEY---AEK 60
Query: 63 IPEGETTTFLMDVARDTGVYIVAGTA-EKDGDVLYNSAVVVGPRG-FIGKYRKIHLF--- 117
IP GE+T L +VA++ +Y++ G+ E+D LYN+ V GP G + KYRKIHLF
Sbjct: 61 IP-GESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDID 119
Query: 118 ------YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPAN 171
++E PGD F FD + +VG+ IC+D F E A+ A +G ++ +P
Sbjct: 120 VPGKITFQESKTLSPGD-SFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGA 178
Query: 172 LVMPYAPRAMPI----RALENKVYTVTADRVGEERGLKFI-GKSLIASPKAEVLSMASET 226
+ P + RA++N+VY TA +++ G S + +P EVL+ A T
Sbjct: 179 FNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAG-T 237
Query: 227 EEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
EE + ++ID L + I +F+ +R + Y
Sbjct: 238 EEAIVYSDID--LKKLAEIRQQIPVFRQKRSDLY 269
>gi|11497735 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7449039|pir||C69264 conserved hypothetical protein
AF0115 - Archaeoglobus fulgidus
>gi|2650534|gb|AAB91113.1| (AE001098) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 257
Score = 129 bits (321), Expect = 3e-29
Identities = 95/264 (35%), Positives = 145/264 (53%), Gaps = 31/264 (11%)
Query: 2 VKVAYVQMNPQILEPDK--NYSKAEKLIKEASKQGAQLVVLPELFDTGY---NFETREEV 56
+++A Q Q + PD+ N K LIK A + A +V+LPE+F+TG+ N+ET E
Sbjct: 7 IRIALAQ---QRILPDREVNIMKGMSLIKRAIQVRADMVILPEVFNTGFYKHNYETVE-- 61
Query: 57 FEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHL 116
P E + L+ ++ + I+ G AE++GD LYNSAV++ IGKYRK HL
Sbjct: 62 -------PLEEELSLLLKISEQKDIMIITGVAEREGDDLYNSAVIIHKGKIIGKYRKTHL 114
Query: 117 F--YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLV- 173
F EK +F+ GD VF+ K+G++IC++ FPE +R L GA++I PA
Sbjct: 115 FPLTNEKKYFKAGD-KLEVFETHLGKIGLLICYEVRFPELSRKLVKMGAEIIVIPAEFPK 173
Query: 174 --MPYAPRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVG 231
+ + + RA+EN+V+ + V E L + G S++ P VL ASE +EV
Sbjct: 174 ERIDHWRVLLQARAIENQVFVAGVNCV--EGDLDYGGHSMLIDPMGTVLIEASE-YQEVM 230
Query: 232 VAEI---DLYLVRNK--RINDLND 250
+++I D+Y VR K +NDL +
Sbjct: 231 MSDIRLGDVYEVRKKFPFLNDLRE 254
>pir||B81369 probable hydrolase Cj0947c [imported] - Campylobacter jejuni
(strain NCTC 11168) >gi|6968384|emb|CAB73204.1|
(AL139076) putative hydrolase [Campylobacter jejuni]
Length = 290
Score = 129 bits (320), Expect = 5e-29
Identities = 95/287 (33%), Positives = 147/287 (51%), Gaps = 34/287 (11%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETRE-EVFEIA 60
+K+A +Q L ++ K + I+EASKQGA+LV L EL + Y ++ + F+ A
Sbjct: 1 MKIALIQQKFH-LNKEQTIKKTCEFIEEASKQGAELVCLGELHQSEYFCQSENVDFFDYA 59
Query: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLY-NSAVVVGPRGFI-GKYRKIHL-- 116
+ F ++AR + ++A EK LY N+AVV G I GKYRK+H+
Sbjct: 60 NDYEKD--VKFWANIARKNQIVLIASLFEKRSAGLYHNTAVVFEKDGSIAGKYRKMHIPD 117
Query: 117 ---FYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLV 173
FY EKF+F PGDLGF + K+GV+IC+D ++PE+AR +ALKGA+++ +P +
Sbjct: 118 DPCFY-EKFYFTPGDLGFEPINTSLGKLGVLICWDQWYPEAARIMALKGAEILIYPTAIG 176
Query: 174 MPYAPR-------------AMPIRALENKVYTVTADRVGEER-------GLKFIGKSLIA 213
+ A+ N +Y V +RVG E+ G++F G S +
Sbjct: 177 WFDKDKDEEKQRQLNAWLGVQKGHAIANGLYVVAINRVGFEKDVSGVEEGIRFWGNSFVF 236
Query: 214 SPKAEVLSMASETEEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
P+ E L + E V + EID R++ + +DRR EY+
Sbjct: 237 GPQGEELCLLDSQNECVKIIEID--KKRSENVRRWWPFLRDRRIEYF 281
>pir||T41662 probable nitrilase - fission yeast (Schizosaccharomyces pombe)
>gi|3136038|emb|CAA19069.1| (AL023590) hypothetical
protein [Schizosaccharomyces pombe]
Length = 272
Score = 128 bits (319), Expect = 6e-29
Identities = 91/268 (33%), Positives = 133/268 (48%), Gaps = 13/268 (4%)
Query: 4 VAYVQMNPQILEPDKNYSKAEKLIKEA--SKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
+A VQM P++ + N K + E S L++ PEL +GY E +IA+
Sbjct: 5 IACVQMAPKVCDVKHNLQKMSSYVHEVMESNPSTNLILFPELITSGY--ECGNTFTQIAE 62
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEKD---GDVLYNSAVVVGPRGFIGK-YRKIHLF 117
EG + + ++A V I+ G EK+ +++YNS + + G +G YRK+HLF
Sbjct: 63 IAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEKQSNIIYNSCIYITENGNLGGVYRKVHLF 122
Query: 118 YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYA 177
E+ F+ G F +F+ F K+GVMIC+D FPE AR AL GAD++ N PY+
Sbjct: 123 DTERKHFKKGS-DFPIFETSFGKLGVMICWDTAFPEVARIHALNGADLLVVATNWENPYS 181
Query: 178 ---PRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAE 234
RA EN + V A+RVG + L F G S I P +V+ E +E V
Sbjct: 182 DDWDLVTKARAFENCIPLVAANRVGTDEKLSFFGHSKIIGPTGKVIKALDEEKEGVISYT 241
Query: 235 IDLYLVRNKRINDLNDIFKDRREEYYFR 262
+DL + R N F+DR + Y R
Sbjct: 242 VDLDDAKPLRKN-YYTFFEDRMPDLYKR 268
>pir||G71949 hypothetical protein jhp0294 - Helicobacter pylori (strain J99)
>gi|4154816|gb|AAD05871.1| (AE001466) putative
[Helicobacter pylori J99]
Length = 294
Score = 127 bits (315), Expect = 2e-28
Identities = 90/265 (33%), Positives = 142/265 (52%), Gaps = 23/265 (8%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVF--- 57
++K A +QM + ++N A L KEA +GA L+VLPELFD+GY ++ F
Sbjct: 10 ILKTAVIQMQSKPYALNENLQLALNLAKEAHNKGANLIVLPELFDSGYCVNDKDADFGLD 69
Query: 58 ----EIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRG-FIGKYR 112
E ++ + ET L D A+ + +IVA + EK+ LY+SA ++ P+G +GK+R
Sbjct: 70 FKAIEHGEETLKNETLRALSDFAKSSDTHIVACSIEKNNKKLYDSAYIIPPKGKIVGKHR 129
Query: 113 KIHLFYREKFFFEPGDLGFRVFDLGF----MKVGVMICFDWFFPESARTLALKGADVIAH 168
KI+L+ EK F+ G + VF L F KVG+ IC++ F A L L+GA+V+ +
Sbjct: 130 KIYLWGDEKSRFKRGK-KYEVFTLDFGDFSAKVGLQICYETGFGVGANLLVLQGAEVLIY 188
Query: 169 PANLVMPYA---PRAMPIRALENKVYTVTADRVGEE------RGLKFIGKSLIASPKAEV 219
P+ A RALEN + + GEE + L+F G S I +P ++
Sbjct: 189 PSAFGKARAYNWDLLSKARALENGCFVCACNHSGEETNAKLKQTLEFAGDSRIIAPNGKI 248
Query: 220 LSMASETEEEVGVAEIDLYLVRNKR 244
++ A++ E + +AE+DL V +R
Sbjct: 249 IAQATKLNEVI-IAEMDLNEVALQR 272
>pir||E64558 conserved hypothetical protein HP0309 - Helicobacter pylori
(strain 26695) >gi|2313405|gb|AAD07375.1| (AE000549)
conserved hypothetical protein [Helicobacter pylori
26695]
Length = 292
Score = 126 bits (314), Expect = 2e-28
Identities = 92/263 (34%), Positives = 140/263 (52%), Gaps = 21/263 (7%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
++K A +QM + ++N A L KEA +GA L+VLPELFD+GY ++ F +
Sbjct: 10 ILKTAVIQMQSKPYALNENLQLALNLAKEAHDKGANLIVLPELFDSGYFVNDKDADFGLD 69
Query: 61 QKIPE-----GETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPR-GFIGKYRKI 114
K E ET L D A+ V++VA + EK LY+SA ++ P+ G +GK+RKI
Sbjct: 70 FKAIEHSELKSETLRALSDFAKSNKVHLVACSIEKTNQKLYDSAYIIPPKVGIVGKHRKI 129
Query: 115 HLFYREKFFFEPGDLGFRVFDLGF----MKVGVMICFDWFFPESARTLALKGADVIAHPA 170
+L+ EK F G + VF L F KVG+ IC++ F A LAL+GA+V+ +P+
Sbjct: 130 YLWGDEKSRFRRGK-KYEVFTLDFGDFSAKVGLQICYEIGFGVGANLLALQGAEVLIYPS 188
Query: 171 NLVMPYA---PRAMPIRALENKVYTVTADRVGEE------RGLKFIGKSLIASPKAEVLS 221
A RALEN + + GEE + L+F G S I +P ++++
Sbjct: 189 AFGKARAYNWDLLSRARALENGCFVCACNHNGEETNAQLKQTLEFAGDSRIIAPNGKIIA 248
Query: 222 MASETEEEVGVAEIDLYLVRNKR 244
A++ E + +AE+DL V +R
Sbjct: 249 QATKLNEVI-IAEMDLNEVALQR 270
>gb|AAF87102.1|AF284573_1 (AF284573) Nit protein 2 [Mus musculus]
Length = 276
Score = 126 bits (313), Expect = 3e-28
Identities = 90/274 (32%), Positives = 146/274 (52%), Gaps = 25/274 (9%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQK 62
++A +Q+ ++ D N ++A L++EA+KQGA +V LPE F++ Y + A+K
Sbjct: 5 RLALIQLQVSSIKSD-NLTRACSLVREAAKQGANIVSLPECFNSPYGTTYFPDY---AEK 60
Query: 63 IPEGETTTFLMDVARDTGVYIVAGTA-EKDGDVLYNSAVVVGPRG-FIGKYRKIHLF--- 117
IP GE+T L +VA+++ +Y++ G+ E+D LYN+ V GP G + K+RKIHLF
Sbjct: 61 IP-GESTQKLSEVAKESSIYLIGGSIPEEDAGKLYNTCSVFGPDGSLLVKHRKIHLFDID 119
Query: 118 ------YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPAN 171
++E PGD F FD + KVG+ IC+D F E A+ A +G ++ +P
Sbjct: 120 VPGKITFQESKTLSPGD-SFSTFDTPYCKVGLGICYDMRFAELAQIYAQRGCQLLVYPGA 178
Query: 172 LVMPYAPRAMPI----RALENKVYTVTADRVGEERGLKFI-GKSLIASPKAEVLSMASET 226
+ P + RA++N+VY TA +++ G S + P +VL+ A T
Sbjct: 179 FNLTTGPAHWELLQRARAVDNQVYVATASPARDDKASYVAWGHSTVVDPWGQVLTKAG-T 237
Query: 227 EEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
EE + ++ID L + I I K +R + Y
Sbjct: 238 EETILYSDID--LKKLAEIRQQIPILKQKRADLY 269
>pir||T28684 hypothetical protein - Streptomyces coelicolor
>gi|3127835|emb|CAA18901.1| (AL023496) hypothetical
protein [Streptomyces coelicolor A3(2)]
Length = 280
Score = 124 bits (308), Expect = 1e-27
Identities = 85/251 (33%), Positives = 137/251 (53%), Gaps = 16/251 (6%)
Query: 24 EKLIKEASKQGAQLVVLPELFDTGYNFETRE-EVFEIAQKIPEGETTTFLMDVARDTGVY 82
E+ +++A+ QGAQ++ ELF Y + ++ E + A+++P+G +AR+ G+
Sbjct: 26 EQAVRDAAAQGAQVMCFQELFYGPYFCQVQDPEFYAYAERVPDGPIVERFQRLAREHGIV 85
Query: 83 IVAGTAEKDGD-VLYNSAVVVGPRG-FIGKYRKIHL----FYREKFFFEPGDLGFRVFDL 136
+V E++ VLYN+A V+ G ++GKYRK H+ + EKF+F PG+ G+ VFD
Sbjct: 86 LVLPMYEEEQPGVLYNTAAVIDADGSYLGKYRKTHIPQVRGFWEKFYFRPGNSGWPVFDT 145
Query: 137 GFMKVGVMICFDWFFPESARTLALKGADVIAHPA----NLVMPYAPRAMPIRALENKVYT 192
KVGV IC+D FPE R L L+GA+++ +P+ L P A+ N+ +
Sbjct: 146 AVGKVGVYICYDRHFPEGWRALGLEGAEIVFNPSATSRGLSGYLWQLEQPAAAVANEYFV 205
Query: 193 VTADRVG-EERG-LKFIGKSLIASPKAE-VLSMASETEEEVGVAEIDLYLVRNKRINDLN 249
+RVG EE G F G S P+A V +AS+ E E+ V ++D + R + + D
Sbjct: 206 GAINRVGVEELGDNDFYGTSYFVDPEARFVGEVASDKETELVVRDLD--MARLREVRDRW 263
Query: 250 DIFKDRREEYY 260
++DR Y
Sbjct: 264 QFYRDRAPGAY 274
>pir||H82556 beta-alanine synthetase XF2443 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9107636|gb|AAF85242.1|AE004053_5
(AE004053) beta-alanine synthetase [Xylella fastidiosa]
Length = 295
Score = 124 bits (308), Expect = 1e-27
Identities = 92/272 (33%), Positives = 145/272 (52%), Gaps = 33/272 (12%)
Query: 17 DKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMD 74
+ N S E + EA+ QGAQLV+L EL ++ Y F E V F++A+ IP G +T L
Sbjct: 20 EANLSIIEARVTEAAAQGAQLVLLHELHNSAY-FCQHESVNEFDLAEPIP-GPSTERLSA 77
Query: 75 VARDTGVYIVAGTAEKDGDVLY-NSAVVVGPRG-FIGKYRKIHL-----FYREKFFFEPG 127
+A+ V I+ EK LY N+AVV+ G +GKYRK+H+ FY EKF+F PG
Sbjct: 78 LAKQHRVVIIGSLFEKRAAGLYHNTAVVLEKDGRLVGKYRKMHIPDDPGFY-EKFYFTPG 136
Query: 128 DLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVM-PYAPRAMPIR-- 184
D+GF+ D ++GV++C+D ++PE+AR +AL GA+++ +P + P R
Sbjct: 137 DIGFKPIDTSVGRLGVLVCWDQWYPEAARLMALAGAELLLYPTAIGWDPNDEHDEQTRQR 196
Query: 185 ----------ALENKVYTVTADRVGEE------RGLKFIGKSLIASPKAEVLSMASETEE 228
A+ N + ++ +R G E G+ F G S + P+ E L+ A+
Sbjct: 197 DAWLLSHRGHAIANSLPVLSCNRTGHEPSPLGTSGIHFWGNSHVLGPQGEFLAEANSNGP 256
Query: 229 EVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
E+ EI+L R++ + + +DRR + Y
Sbjct: 257 EILTCEINLQ--RSEHVRRIWPFLRDRRIDAY 286
>gi|9631647 contains ATP/GTP-binding site motif A; similar to rat beta-alanine
synthetase, corresponds to Swiss-Prot Accession Number
Q03248 [Paramecium bursaria Chlorella virus 1]
>gi|7461248|pir||T17568 hydrolase homolog A78R -
Chlorella virus PBCV-1 >gi|624085|gb|AAC96446.1|
(U42580) contains ATP/GTP-binding site motif A; similar
to rat beta-alanine synthetase, corresponds to
Swiss-Prot Accession Number Q03248 [Paramecium bursaria
Chlorella virus 1]
Length = 298
Score = 122 bits (302), Expect = 6e-27
Identities = 90/277 (32%), Positives = 132/277 (47%), Gaps = 35/277 (12%)
Query: 19 NYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETRE-EVFEIAQKIPEGETTTFLMDVAR 77
N +AE L++ A+ GAQ++VL ELF T Y +T+ + F+ A + +A+
Sbjct: 21 NIERAEMLVRNAAANGAQVIVLQELFATKYFCQTQSPQYFKFADPADDSVIVEIFSKLAK 80
Query: 78 DTGVYIVAGTAEKDGDVLYNSAVVVGPRG-FIGKYRKIHL----FYREKFFFEPGDLGFR 132
+ GV I EKDG+ YNS V G +G YRK H+ Y EKF+F P +
Sbjct: 81 ELGVVIPIPFFEKDGNNYYNSVAVADADGSIVGVYRKTHIPQSKCYEEKFYFTPSSNPYE 140
Query: 133 VFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANL-VMPYAP---------RAMP 182
VF+ F K+GV+IC+D +F E+A+ LAL+GAD I +P + P P R +
Sbjct: 141 VFETKFGKMGVLICWDQWFSEAAKCLALEGADFIVYPTAIGSEPEFPNGESYLHWARTIT 200
Query: 183 IRALENKVYTVTADRVGEER----GLKFIGKSLIASPKAEVLSMASETEEEVGVAE---- 234
A V + A+RVG ER + F G S IA V++ ++ G +
Sbjct: 201 GHAAATGVPVIVANRVGRERFGKTKIDFFGGSFIADGTGAVVTQVGGVPQKNGGVDPEPV 260
Query: 235 ---------IDLYLVRNKRINDLNDIFKDRREEYYFR 262
DL + N R +++DRR E Y R
Sbjct: 261 DLKGYTKYTFDLDALENHRA--FWGLYRDRRPELYSR 295
>pir||T34905 probable hydrolase - Streptomyces coelicolor
>gi|3218363|emb|CAA19622.1| (AL023861) putative
hydrolase [Streptomyces coelicolor A3(2)]
Length = 280
Score = 119 bits (296), Expect = 3e-26
Identities = 83/254 (32%), Positives = 137/254 (53%), Gaps = 16/254 (6%)
Query: 21 SKAEKLIKEASKQGAQLVVLPELFDTGYNFETRE-EVFEIAQKIPEGETTTFLMDVARDT 79
+K E+ +EA+++GA+++ E+F+ Y + ++ E + A+ +P+G T + +AR+T
Sbjct: 23 AKHEEHAREAARRGARVIGFQEVFNAPYFCQVQDPEHYRWAEPVPDGPTVRRMQALARET 82
Query: 80 GVYIVAGTAE-KDGDVLYNSAVVVGPRGFI-GKYRKIHL----FYREKFFFEPGDLGFRV 133
G+ IV E + YN+A V+ G + G YRK H+ + EKF+F PG+ G+ V
Sbjct: 83 GMVIVVPVFEVEQSGFYYNTAAVIDADGTVLGTYRKHHIPQVKGFWEKFYFRPGNAGWPV 142
Query: 134 FDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPA----NLVMPYAPRAMPIRALENK 189
FD KVGV IC+D FPE R L L GA ++ +P+ L P A+ N+
Sbjct: 143 FDTAVGKVGVYICYDRHFPEGWRQLGLGGAQLVYNPSATHRGLSAHLWRLEQPAAAVANE 202
Query: 190 VYTVTADRVG-EERG-LKFIGKSLIASPKAE-VLSMASETEEEVGVAEIDLYLVRNKRIN 246
+ +RVG EE G F G S P+ + V +AS+++EE+ V ++D L+ +
Sbjct: 203 YFVAAINRVGVEEYGDNDFYGTSYFVDPRGQFVGDVASDSKEELVVRDLDFDLI--DEVR 260
Query: 247 DLNDIFKDRREEYY 260
++DRR + Y
Sbjct: 261 QQWAFYRDRRPDAY 274
>dbj|BAB04766.1| (AP001510) unknown conserved protein [Bacillus halodurans]
Length = 271
Score = 118 bits (293), Expect = 7e-26
Identities = 82/242 (33%), Positives = 132/242 (53%), Gaps = 23/242 (9%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQG--AQLVVLPELFDTGYNFETREEVFEI 59
+KVA QM+ +P N K ++ I++ +Q L+VLPE++ T Y + E + E
Sbjct: 1 MKVALYQMDILPGDPRGNERKVKEWIEDVMQQEDVPDLLVLPEMWTTAYTLDQLEHLAEG 60
Query: 60 AQKIPEGETTTFLMDVARDTGVYIVAGT-AEKDGDVLYNSAVVVGPRGF-IGKYRKIHL- 116
++ E FL ++AR+ V IVAG+ A+K+ LYN A+V RG + +Y KIHL
Sbjct: 61 EERYTE----LFLKELAREHNVNIVAGSIAKKEKGKLYNRALVFDRRGHTVYQYDKIHLV 116
Query: 117 -FYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMP 175
E + GD VF+L K+G++IC+D FPE R+LAL+GA+++ ++
Sbjct: 117 PMLSEPDYLTGGDAAASVFELEGTKMGLVICYDLRFPELMRSLALEGAEIV-----FIVA 171
Query: 176 YAPRAMPI--------RALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETE 227
P A + RA+EN+ Y ++ +RVG G+ F G+S++ P +VL S +
Sbjct: 172 EWPEARAVHWEVLQRARAIENQSYVISCNRVGAYAGVTFAGRSMVIDPWGDVLIQGSRDQ 231
Query: 228 EE 229
E+
Sbjct: 232 EQ 233
>pir||H83195 conserved hypothetical protein PA3598 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9949754|gb|AAG06986.1|AE004780_5 (AE004780)
conserved hypothetical protein [Pseudomonas aeruginosa]
Length = 271
Score = 118 bits (292), Expect = 9e-26
Identities = 83/247 (33%), Positives = 130/247 (52%), Gaps = 10/247 (4%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
+KV VQ+ + + N +A I + + +LVV PE TG F + + + +A+
Sbjct: 1 MKVELVQIAGRDGDTAHNLERALAAIADCAAD-TELVVFPETHLTG--FPSEDNIAALAE 57
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYREK 121
+ +G T + + VAR+ V + G AE D YN+ +++ P G KYRK HL+ ++
Sbjct: 58 PL-DGPTVSAVQRVARERNVSVAIGIAEADAGRYYNTTLLIAPDGIALKYRKTHLWASDR 116
Query: 122 FFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAP--- 178
F PGD G ++VG+++CFD FPESAR L GA++I + PY P
Sbjct: 117 GIFTPGDRYATALWNG-IRVGLLVCFDIEFPESARALGQLGAELIIVTNGNMDPYGPTHR 175
Query: 179 RAMPIRALENKVYTVTADRVGE-ERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDL 237
A+ RA+EN+ Y V +RVG + GL F G S + P ++L A EE + E+DL
Sbjct: 176 TAIMARAMENQAYAVMVNRVGHGDGGLVFAGGSAVVDPYGQLLCEAGR-EECRQIVELDL 234
Query: 238 YLVRNKR 244
+++ R
Sbjct: 235 GRLQDAR 241
>gb|AAD15597.1| (AC006232) putative nitrilase [Arabidopsis thaliana]
Length = 220
Score = 116 bits (289), Expect = 2e-25
Identities = 72/212 (33%), Positives = 113/212 (52%), Gaps = 25/212 (11%)
Query: 72 LMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGF-IGKYRKIHL----FYREKFFFEP 126
+ +A++ GV I E+ YNS ++ G +G YRK H+ Y+EKF+F P
Sbjct: 1 MQKLAKELGVVIPVSFFEEANTAHYNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNP 60
Query: 127 GDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANL----------VMPY 176
GD GF+VF F K+GV IC+D +FPE+AR + L+GA+++ +P + +
Sbjct: 61 GDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTAIGSEPQDQGLDSRDH 120
Query: 177 APRAMPIRALENKVYTVTADRVGEE--------RGLKFIGKSLIASPKAEVLSMASETEE 228
R M A N V V ++R+G+E + F G S IA P E+++ A + E
Sbjct: 121 WRRVMQGHAGANVVPLVASNRIGKEIIETEHGPSQITFYGTSFIAGPTGEIVAEADDKSE 180
Query: 229 EVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
V VA+ DL ++++KR +F+DRR + Y
Sbjct: 181 AVLVAQFDLDMIKSKR--QSWGVFRDRRPDLY 210
>emb|CAB76877.1| (AL159139) putative hydrolase [Streptomyces coelicolor A3(2)]
Length = 262
Score = 115 bits (286), Expect = 4e-25
Identities = 80/264 (30%), Positives = 131/264 (49%), Gaps = 11/264 (4%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
++ A +Q + + +N ++ A+ GA L+V ELF TGY + +
Sbjct: 1 MRTALLQSSGRPGSTAENLEVLDEAAGRAAAAGAALLVTSELFLTGYAIG---DAVGVLA 57
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGF-IGKYRKIHLF-YR 119
+ +G+ + ++A G+ + G E+DG+ ++NS ++ G + YRK HLF
Sbjct: 58 EPADGDAADAVAEIAGRHGLAVAYGYPERDGERVFNSVQLIAADGTRLAGYRKTHLFGCF 117
Query: 120 EKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPY--- 176
E+ F PG+ +L + VG+MIC+D FPE+ R AL G D++ P + P+
Sbjct: 118 ERDHFTPGEQPVVQAELNGLTVGLMICYDVEFPENVRAHALAGTDLLLVPTAQMHPFQFV 177
Query: 177 APRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEID 236
A +P+RA EN++Y DRVG E +F+G S +A P + A EE + +A+ D
Sbjct: 178 AESVVPVRAFENQMYVAYVDRVGREGEFEFVGLSTLAGPDGVARARAGRGEELI-LADAD 236
Query: 237 LYLVRNKRINDLNDIFKDRREEYY 260
+ R + N KDRR Y
Sbjct: 237 PAFLAASR--EANPYLKDRRPGLY 258
>sp|P55176|YPQQ_PSEFL HYPOTHETICAL 31.2 KD PROTEIN IN PQQF 5'REGION (ORF2)
>gi|2120658|pir||S58240 hypothetical protein 2 -
Pseudomonas fluorescens >gi|929801|emb|CAA60729.1|
(X87299) unidentified ORF2 [Pseudomonas fluorescens]
Length = 285
Score = 114 bits (283), Expect = 1e-24
Identities = 86/265 (32%), Positives = 140/265 (52%), Gaps = 13/265 (4%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
++VA Q P+ L+ N + ++ EA+ A L+VLPE+F +GYN E V +A+
Sbjct: 22 MRVALYQCPPRPLDVAGNLQRLHQVAMEATD--ADLLVLPEMFLSGYNIGL-EAVGALAE 78
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEKDGD-VLYNSAVVVGPRGF-IGKYRKIHLFYR 119
+G + + +A+ G I+ G E+ D +YN+ ++ +G + YRK HLF
Sbjct: 79 A-QDGPSAQRIAAIAQAAGTAILYGYPERSVDGQIYNAVQLIDAQGQRLCNYRKTHLFGD 137
Query: 120 -EKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPY-- 176
+ F G+ F + +L K+G +IC+D FPE+AR LAL GA++I P ++PY
Sbjct: 138 LDHSMFSAGEDDFPLVELDGWKLGFLICYDIEFPENARRLALAGAELILVPTANMIPYDF 197
Query: 177 -APRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEI 235
A + RA EN+ Y A+ G E +++ G+S IA+P +++A +E + + +
Sbjct: 198 VADVTIRARAFENQCYVAYANYCGHEEQIRYCGQSSIAAPDGSRIALAG-LDEALIIGTL 256
Query: 236 DLYLVRNKRINDLNDIFKDRREEYY 260
D L+ R LN DRR E Y
Sbjct: 257 DRQLMGESRA--LNRYLSDRRPELY 279
>emb|CAA62550.1| (X91070) D-N-alpha-carbamilase [Agrobacterium radiobacter]
>gi|1589569|prf||2211348A carbamoyl-D-AA amidohydrolase
[Agrobacterium radiobacter]
Length = 304
Score = 110 bits (272), Expect = 2e-23
Identities = 83/270 (30%), Positives = 132/270 (48%), Gaps = 36/270 (13%)
Query: 26 LIKEASKQGAQLVVLPEL----FDTGYNFETREEVFEIAQKIPEGETTTFLMDVARDTGV 81
++ A+ +G +V PEL F ++F E+ + G L + A + G+
Sbjct: 31 MLTNAASRGVNFIVFPELALTTFFPRWHFTDEAELDSFYETEMPGPVVRPLFETAAELGI 90
Query: 82 YIVAGTAEK--DGDVL--YNSAVVVGPRG-FIGKYRKIHL-------FYR-----EKFFF 124
G AE +G V +N++++V G +GKYRKIHL YR EK +F
Sbjct: 91 GFNLGYAELVVEGGVKRRFNTSILVDKSGKIVGKYRKIHLPGHKEYEAYRPFQHLEKRYF 150
Query: 125 EPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVM--PYAPR--- 179
EPGDLGF V+D+ K+G+ IC D +PE+ R + LKGA++I N P P+
Sbjct: 151 EPGDLGFPVYDVDAAKMGMFICNDRRWPETWRVMGLKGAEIICGGYNTPTHNPPVPQHDH 210
Query: 180 --------AMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVG 231
+M + +N ++ A +VG E G +G S I +P E++++ + E+EV
Sbjct: 211 LTSFHHLLSMQAGSYQNGAWSAAAGKVGMEEGCMLLGHSCIVAPTGEIVALTTTLEDEVI 270
Query: 232 VAEIDLYLVRNKRINDLNDIFKDRREEYYF 261
A +DL R R + N FK R+ ++
Sbjct: 271 TAAVDLDRCRELREHIFN--FKAHRQPQHY 298
>gb|AAB47607.1| (U59376) N-carbamoyl-D-amino acid amidohydrolase [Agrobacterium
radiobacter]
Length = 304
Score = 109 bits (271), Expect = 3e-23
Identities = 83/270 (30%), Positives = 132/270 (48%), Gaps = 36/270 (13%)
Query: 26 LIKEASKQGAQLVVLPEL----FDTGYNFETREEVFEIAQKIPEGETTTFLMDVARDTGV 81
++ A+ +G +V PEL F ++F E+ + G L + A + G+
Sbjct: 31 MLTNAASRGVNFIVFPELALTTFFPRWHFTDEAELDSFYETEMPGPVVRPLFETAAELGI 90
Query: 82 YIVAGTAEK--DGDVL--YNSAVVVGPRG-FIGKYRKIHL-------FYR-----EKFFF 124
G AE +G V +N++++V G +GKYRKIHL YR EK +F
Sbjct: 91 GFNLGYAELVVEGGVKRRFNTSILVDKSGKIVGKYRKIHLPGHKEYEAYRPFQHLEKRYF 150
Query: 125 EPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVM--PYAPR--- 179
EPGDLGF V+D+ K+G+ IC D +PE+ R + LKGA++I N P P+
Sbjct: 151 EPGDLGFPVYDVDAAKMGMFICNDRRWPETWRVMGLKGAEIICGGYNTPTHNPPVPQHDH 210
Query: 180 --------AMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVG 231
+M + +N ++ A +VG E G +G S I +P E++++ + E+EV
Sbjct: 211 LTSFHHLLSMQAGSYQNGAWSAAAGKVGMEEGCMLLGHSCIVAPTGEIVALTTTLEDEVI 270
Query: 232 VAEIDLYLVRNKRINDLNDIFKDRREEYYF 261
A +DL R R + N FK R+ ++
Sbjct: 271 TAALDLDRCRELREHIFN--FKAHRQPQHY 298
>pir||JW0082 N-carbamyl-D-amino acid amidohydrolase (EC 3.5.-.-) - Agrobacterium
sp
Length = 304
Score = 108 bits (268), Expect = 6e-23
Identities = 82/270 (30%), Positives = 133/270 (48%), Gaps = 36/270 (13%)
Query: 26 LIKEASKQGAQLVVLPEL----FDTGYNFETREEVFEIAQKIPEGETTTFLMDVARDTGV 81
++ +A+ +GA +V PEL F ++F E+ + G L + A + G+
Sbjct: 31 MLTKAASRGANFIVFPELALTTFFPRWHFTDEAELDSFYETEMPGPVVRPLFEKAAELGI 90
Query: 82 YIVAGTAEK--DGDVL--YNSAVVVGPRG-FIGKYRKIHL-------FYR-----EKFFF 124
G AE +G V +N++++V G +GKYRKIHL YR EK +F
Sbjct: 91 GFNLGYAELVVEGGVKRRFNTSILVDKSGKIVGKYRKIHLPGHKEYEAYRPFQHLEKRYF 150
Query: 125 EPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVM--PYAPR--- 179
EPGDLGF V+D+ K+G+ IC D +PE+ R + L+GA++I N P P+
Sbjct: 151 EPGDLGFPVYDVDAAKMGMFICNDRRWPEAWRVMGLRGAEIICGGYNTPTHNPPVPQHDH 210
Query: 180 --------AMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVG 231
+M + +N ++ A +VG E +G S I +P E++++ + E+EV
Sbjct: 211 LTSFHHLLSMQAGSYQNGAWSAAAGKVGMEENCMLLGHSCIVAPTGEIVALTTTLEDEVI 270
Query: 232 VAEIDLYLVRNKRINDLNDIFKDRREEYYF 261
A +DL R R + N FK R+ ++
Sbjct: 271 TAAVDLDRCRELREHIFN--FKQHRQPQHY 298
>pir||B69109 N-carbamoyl-D-amino acid amidohydrolase - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622943|gb|AAB86277.1| (AE000934)
N-carbamoyl-D-amino acid amidohydrolase
[Methanobacterium thermoautotrophicum]
Length = 272
Score = 105 bits (260), Expect = 5e-22
Identities = 84/262 (32%), Positives = 131/262 (49%), Gaps = 24/262 (9%)
Query: 15 EPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMD 74
+ +KN A ++I EA+++GA+L+VLPE+F Y+ E E G + +
Sbjct: 13 DKEKNVGTAVEMIVEAAERGARLIVLPEMFTCPYDVNLFSEYAE----DENGYSIRTMRS 68
Query: 75 VARDTGVYIVAGT-AEKDGDVLYNSAVVVGPRG-FIGKYRKIHLF---------YREKFF 123
+AR+ G+++VAG+ E+ + +YN++ V+ G IGK+RK+HLF +RE
Sbjct: 69 IARELGIHLVAGSIPEETPEGIYNTSFVMDDGGNIIGKHRKVHLFDINVPGEISFRESDS 128
Query: 124 FEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPI 183
GD V + +GV IC+D FPE +R +AL GA+V+ P M P +
Sbjct: 129 LIAGD-SVTVIETPQCVMGVGICYDMRFPELSRMMALGGAEVLIFPGAFNMTTGPAHWRL 187
Query: 184 ----RALENKVYTVTADRV-GEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLY 238
RAL+N+ Y V A G S+IA P V+ A + V A+IDL
Sbjct: 188 LVRSRALDNQCYCVAASPARNPSASYVAYGHSMIADPWGSVVCEA-DASPSVITADIDLE 246
Query: 239 LVRNKRINDLNDIFKDRREEYY 260
V +RI + ++RR + Y
Sbjct: 247 AV--ERIRRKLPLLRNRRPDVY 266
>emb|CAC12333.1| (AL445066) nitrilase related protein [Thermoplasma acidophilum]
Length = 270
Score = 105 bits (259), Expect = 6e-22
Identities = 84/270 (31%), Positives = 138/270 (51%), Gaps = 22/270 (8%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
+KVA VQM + +KN + +L+++A + + LVV PE F+ ++++ I++
Sbjct: 1 MKVAVVQMESST-DREKNIEASYRLLEKA--KNSDLVVFPEYQIYAPAFDGKDDMKTISE 57
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVL--YNSAVVVGPRGFIGKYRKIHLF-- 117
+ +G+ + ++AR I+ E++ L +N+A+ + G I KYRK+HLF
Sbjct: 58 PL-DGKFVKSITEIARSESQKIILNIPERNQYNLKPFNTAIYIDELGLILKYRKLHLFDA 116
Query: 118 --YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLV-- 173
+RE FE GD +F+ +GV+IC+D FPE AR LAL GA +I + A
Sbjct: 117 FGFRESSVFEKGDARPAIFNGSGDPLGVLICYDLRFPEPARMLALDGAKLIIYQAGWFAG 176
Query: 174 ---MPYAPRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEV 230
+ RA+EN V+ + A + G +F G S++ SP +VL+ T+E V
Sbjct: 177 ERKYDQWKTLLKARAMENGVFVIGAAQTGH----RFTGHSMVISPYGDVLAEMG-TDEGV 231
Query: 231 GVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
+ID LV R + + K RR + Y
Sbjct: 232 ITFDIDFSLVEKYR--EEVPVLKHRRTDVY 259
>pir||E64614 beta-alanine synthetase homolog - Helicobacter pylori (strain
26695) >gi|2313883|gb|AAD07805.1| (AE000588)
beta-alanine synthetase homolog [Helicobacter pylori
26695]
Length = 292
Score = 104 bits (257), Expect = 1e-21
Identities = 89/274 (32%), Positives = 136/274 (49%), Gaps = 37/274 (13%)
Query: 18 KNYSKAEKLIKEASKQGAQ--LVVLPELFDTGYNFETRE-EVFEIAQKIPEGETTTFLMD 74
K L+++A K+ + LVVL EL Y ++ + F++ + E + F
Sbjct: 17 KTIEHTANLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKA--FFSA 74
Query: 75 VARDTGVYIVAGTAEKDGDVLY-NSAVVVGPRGFI-GKYRKIHL-----FYREKFFFEPG 127
+A+ V +VA EK LY NSAVV G I G YRK+H+ FY EKF+F PG
Sbjct: 75 LAQKFQVVLVASLFEKRAKGLYHNSAVVFEKDGSIAGVYRKMHIPDDPGFY-EKFYFTPG 133
Query: 128 DLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHP---------ANLVMPYAP 178
DLGF K+G+M+C+D ++PE+AR +ALKGA+++ +P +N
Sbjct: 134 DLGFEPIVTSVGKLGLMVCWDQWYPEAARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQ 193
Query: 179 RAMPI----RALENKVYTVTADRVGEE--------RGLKFIGKSLIASPKAEVLSMASET 226
A A+ N + + +RVG E G+ F G S + E L+ AS+
Sbjct: 194 NAWETIQRGHAIANGLPLIATNRVGVELDPSGAIKGGITFFGSSFVVGALGEFLAKASD- 252
Query: 227 EEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
+EE+ AEID L R + + + +DRR ++Y
Sbjct: 253 KEEILYAEID--LERTEEVRRMWPFLRDRRIDFY 284
>pir||F71901 hypothetical protein jhp0694 - Helicobacter pylori (strain J99)
>gi|4155248|gb|AAD06269.1| (AE001500) putative
[Helicobacter pylori J99]
Length = 292
Score = 102 bits (253), Expect = 3e-21
Identities = 87/274 (31%), Positives = 136/274 (48%), Gaps = 37/274 (13%)
Query: 18 KNYSKAEKLIKEASKQGAQ--LVVLPELFDTGYNFETRE-EVFEIAQKIPEGETTTFLMD 74
K L+++A K+ + LVVL EL Y ++ + F++ + E + F
Sbjct: 17 KTIEHTANLLEQALKKHPKTNLVVLQELNPYSYFCQSENPKFFDLGEYFEEDKA--FFSA 74
Query: 75 VARDTGVYIVAGTAEKDGDVLY-NSAVVVGPRGFI-GKYRKIHL-----FYREKFFFEPG 127
+A+ V ++A EK LY NSAVV G I G YRK+H+ FY EKF+F PG
Sbjct: 75 LAQKFQVVLIASLFEKRAKGLYHNSAVVFEKDGSIAGVYRKMHIPDDPGFY-EKFYFTPG 133
Query: 128 DLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHP---------ANLVMPYAP 178
DLGF K+G+M+C+D ++PE+AR +ALKGA+++ +P +N
Sbjct: 134 DLGFEPIATSVGKLGLMVCWDQWYPEAARIMALKGAEILIYPSAIGFLEEDSNEEKKRQQ 193
Query: 179 RAMPI----RALENKVYTVTADRVGEE--------RGLKFIGKSLIASPKAEVLSMASET 226
A A+ N + + +RVG E G+ F G S + E L+ AS+
Sbjct: 194 NAWETIQRGHAIANGLPLIATNRVGVELDPSGAIKGGITFFGSSFVVGALGEFLAKASD- 252
Query: 227 EEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
+EE+ AEI+ L R + + + +DRR ++Y
Sbjct: 253 KEEILYAEIE--LERTEEVRRMWPFLRDRRIDFY 284
>pir||E69863 conserved hypothetical protein ykrU - Bacillus subtilis
>gi|2633728|emb|CAB13230.1| (Z99111) similar to
hypothetical proteins [Bacillus subtilis]
Length = 259
Score = 98.7 bits (242), Expect = 6e-20
Identities = 81/267 (30%), Positives = 134/267 (49%), Gaps = 22/267 (8%)
Query: 4 VAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKI 63
++ +Q + +P +N KAE I++ SK A ++VLPEL+ TGY+ +E+ +
Sbjct: 5 ISCLQFDISYGKPSENIKKAEFFIEKESKH-ADVLVLPELWTTGYDLANLDELAD----- 58
Query: 64 PEGETT-TFLMDVARDTGVYIVAGTA--EKDGDVLYNSAVVVGPRG-FIGKYRKIHLFYR 119
+G + ++L A+ GV+IVAG+ K+ DV YN+ + G I +YRK HLF
Sbjct: 59 EDGRSAQSWLKKTAKKHGVHIVAGSVAVRKNSDV-YNTMYIADKEGQIIKEYRKAHLFQL 117
Query: 120 --EKFFFEPG-DLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMP- 175
E + G + G+ F+L +K +IC+D FPE R KGA+V+ A +P
Sbjct: 118 MDEHLYLSAGSEDGY--FELDGVKSSGLICYDIRFPEWIRKHTTKGANVLFISAEWPLPR 175
Query: 176 --YAPRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVA 233
+ + RA+EN+ + + G +F G SLI P VL +E E G+
Sbjct: 176 LDHWKSLLIARAIENQCFVAACNCTGSNPDNEFAGHSLIIDPWGRVL---AEGGREEGIV 232
Query: 234 EIDLYLVRNKRINDLNDIFKDRREEYY 260
++ L + + + +F D R++ Y
Sbjct: 233 RAEIDLQESAEVRESIPVFDDIRKDLY 259
>pir||JW0083 N-carbamyl-D-amino acid amidohydrolase (EC 3.5.-.-) - Pseudomonas
sp
Length = 312
Score = 97.9 bits (240), Expect = 1e-19
Identities = 86/297 (28%), Positives = 138/297 (45%), Gaps = 39/297 (13%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLI---KEASKQGAQLVVLPEL----FDTGYNFETR 53
+V A QM P I + +LI +EA +G+ LVV EL F + E
Sbjct: 4 IVNAAAAQMGP-ISRSETRKDTVRRLIALMREAKARGSDLVVFTELALTTFFPRWVIEDE 62
Query: 54 EEVFEIAQKIPEGETTTFLMDVAR--DTGVYI-VAGTAEKDGDVL-YNSAVVVGPRG-FI 108
E+ +K G T L D A+ + G Y+ A AE+ G +N++++V G +
Sbjct: 63 AELDSFYEKEMPGPETQPLFDEAKRLEIGFYLGYAELAEEGGRKRRFNTSILVDRSGRIV 122
Query: 109 GKYRKIHL------------FYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESAR 156
GKYRK+HL + EK +FEPGDLGF V+ +G+ IC D +PE+ R
Sbjct: 123 GKYRKVHLPGHKEPQPGRKHQHLEKRYFEPGDLGFGVWRAFDGVMGMCICNDRRWPETYR 182
Query: 157 TLALKGADVIAHPAN-------------LVMPYAPRAMPIRALENKVYTVTADRVGEERG 203
+ L+G +++ N L + +M A +N + + + G E G
Sbjct: 183 VMGLQGVEMVMLGYNTPYDHTGHDDIDSLTQFHNHLSMQAGAYQNSTWVIGTAKCGTEEG 242
Query: 204 LKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
K +G+S+I +P E+++MA E+E+ A DL + + R + D + R + Y
Sbjct: 243 SKMVGQSVIVAPSGEIVAMACTIEDEIITARCDLDMGKRYR-ETIFDFARHREPDAY 298
>pir||B72408 conserved hypothetical protein - Thermotoga maritima (strain MSB8)
>gi|4980679|gb|AAD35276.1|AE001703_5 (AE001703)
conserved hypothetical protein [Thermotoga maritima]
Length = 267
Score = 96.3 bits (236), Expect = 3e-19
Identities = 78/255 (30%), Positives = 127/255 (49%), Gaps = 14/255 (5%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
++VA VQM P I + + N + E+ I+ A +GA++VV PEL +GY ++ E + +
Sbjct: 1 MRVAAVQMLPAIGDFEGNLERIEQFIEMAVSEGAEVVVFPELTISGYTWD--EAILKRGA 58
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYREK 121
L+ ++R+ + I GT L NS V+ + + Y K HLF EK
Sbjct: 59 LFFSEVAKKKLLKLSREGQILIAVGTPRVVLGKLRNSLVIFKKKKELLFYDKTHLFRGEK 118
Query: 122 FFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVI------AHPANLVMP 175
FEPG+ F VF + G +IC++ FPE +R L KG+ VI
Sbjct: 119 NVFEPGEY-FLVFSYRGVVFGTLICYEIGFPEISRILTFKGSKVILSAFAFGKARGHTYD 177
Query: 176 YAPRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEI 235
A R+ RA+EN V+ V + G E ++F+G++ I P +V++ E+ E + ++
Sbjct: 178 VATRS---RAVENGVFLVASSMCG-EGFVEFVGRTRIVGPNGKVMN-EIESGEGLICEDL 232
Query: 236 DLYLVRNKRINDLND 250
DL V + R ++ D
Sbjct: 233 DLDAVYHYRYDEEGD 247
>gb|AAF54370.1| (AE003682) CG8132 gene product [Drosophila melanogaster]
Length = 283
Score = 96.3 bits (236), Expect = 3e-19
Identities = 81/258 (31%), Positives = 128/258 (49%), Gaps = 29/258 (11%)
Query: 21 SKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDVARDTG 80
+K E +KE + L+ LPE F+ Y + E E IP+G T+ L ++AR
Sbjct: 30 TKIEAAVKEHKPR---LITLPECFNAPYGTKYFREYSET---IPDGYTSQQLSNLARKHQ 83
Query: 81 VYIVAGTAEKDG--DVLYNSAVVVGPRG-FIGKYRKIHLF---------YREKFFFEPGD 128
VYIV GT + G D +YN+ V P G + K+RK+HLF ++E G+
Sbjct: 84 VYIVGGTIPELGENDAIYNTCTVWSPTGDLVAKHRKMHLFDIDVKGGIRFKESETLSAGN 143
Query: 129 LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPI----R 184
F + ++ K+G+ IC+D F E AR G ++I +PA M P + R
Sbjct: 144 -DFTIINVDGHKIGIGICYDIRFEEMARLYRNAGCEMIIYPAAFNMTTGPLHWELLQRSR 202
Query: 185 ALENKVYTVTADRVGEERGLKFI--GKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRN 242
A +N+++ VT + +++ G S++ +P A+V ASE EE V VA+ID V
Sbjct: 203 ANDNQLFVVTTS-PARDTSAEYVAYGHSMVVNPWAKVQQSASEGEEIV-VADIDFSEV-- 258
Query: 243 KRINDLNDIFKDRREEYY 260
+++ +F RR + Y
Sbjct: 259 EQVRQQIPVFGQRRLDLY 276
>pir||T16068 hypothetical protein F13H8.7 - Caenorhabditis elegans
>gi|722377|gb|AAC46683.1| (U23139) highly similar to
beta-ureidopropionase (SP:BUP_RAT) [Caenorhabditis
elegans]
Length = 387
Score = 94.8 bits (232), Expect = 9e-19
Identities = 80/278 (28%), Positives = 131/278 (46%), Gaps = 37/278 (13%)
Query: 1 MVKVAYVQ-------MNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETR 53
+V+VA +Q + + + D + + +I+ A+ GA ++ L E + + F TR
Sbjct: 73 LVRVAAIQNKIHRPTTDSVVEQRDAIHQRVGAMIEAAASAGANVIGLQEAWTMPFAFCTR 132
Query: 54 EEV--FEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKD---GDVLYNSAVVVGPRG-F 107
E + E A+ + G TT FL +A + I++ E+D DV++N+AVV+ G
Sbjct: 133 ERLPWTEFAESVYTGPTTQFLSKLAVKHDIVIISPILERDEEKDDVIWNTAVVISHTGRV 192
Query: 108 IGKYRKIHL----FYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
IG+ RK H+ + E ++ LG VF+ + ++G+ IC+ P++ AL GA
Sbjct: 193 IGRSRKNHIPRVGDFNESTYYMESTLGHPVFETKYGRIGINICYGRHHPQNWMMYALNGA 252
Query: 164 DVIAHPANLVMPYAPRAMPIR----ALENKVYTVTADRVGEE---------RGL------ 204
++I +P+ V + I A+ N V+TV +RVG E G
Sbjct: 253 EIIFNPSATVGALSEPLWGIEARNAAIANHVFTVGINRVGTEVFPNEFTSGNGQPAHKDF 312
Query: 205 -KFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVR 241
F G S IA+P S E V +AE+DL L R
Sbjct: 313 GHFYGSSYIAAPDGSRTPALSRVREGVLIAELDLNLCR 350
>gi|7706509 beta-ureidopropionase [Homo sapiens]
>gi|6288771|gb|AAF06735.1|AF163312_1 (AF163312)
beta-ureidopropionase [Homo sapiens]
>gi|6635205|dbj|BAA88634.1| (AB013885)
beta-ureidopropionase [Homo sapiens]
Length = 384
Score = 94.0 bits (230), Expect = 2e-18
Identities = 74/273 (27%), Positives = 133/273 (48%), Gaps = 32/273 (11%)
Query: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMDVAR 77
+ + + +++ A+ G ++ E + + F TRE++ E A+ +G TT F +A+
Sbjct: 97 HRRIKAIVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAK 156
Query: 78 DTGVYIVAGTAEKD---GDVLYNSAVVVGPRGFI-GKYRKIHL----FYREKFFFEPGDL 129
+ + +V+ E+D GDVL+N+AVV+ G + GK RK H+ + E ++ G+L
Sbjct: 157 NHDMVVVSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNL 216
Query: 130 GFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPIR----A 185
G VF F ++ V IC+ P + ++ GA++I +P+ + + PI A
Sbjct: 217 GHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESLWPIEARNAA 276
Query: 186 LENKVYTVTADRVGEER---------GLK-------FIGKSLIASPKAEVLSMASETEEE 229
+ N +T +RVG E G K F G S +A+P + S + +
Sbjct: 277 IANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSRTPGLSRSRDG 336
Query: 230 VGVAEIDLYLVRNKRINDLNDIFKDRREEYYFR 262
+ VA++DL L +++ND+ + R E Y R
Sbjct: 337 LLVAKLDLNLC--QQVNDVWNFKMTGRYEMYAR 367
>gi|11417872 beta-ureidopropionase [Homo sapiens]
Length = 404
Score = 94.0 bits (230), Expect = 2e-18
Identities = 74/273 (27%), Positives = 133/273 (48%), Gaps = 32/273 (11%)
Query: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMDVAR 77
+ + + +++ A+ G ++ E + + F TRE++ E A+ +G TT F +A+
Sbjct: 117 HRRIKAIVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAK 176
Query: 78 DTGVYIVAGTAEKD---GDVLYNSAVVVGPRGFI-GKYRKIHL----FYREKFFFEPGDL 129
+ + +V+ E+D GDVL+N+AVV+ G + GK RK H+ + E ++ G+L
Sbjct: 177 NHDMVVVSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNL 236
Query: 130 GFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPIR----A 185
G VF F ++ V IC+ P + ++ GA++I +P+ + + PI A
Sbjct: 237 GHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESLWPIEARNAA 296
Query: 186 LENKVYTVTADRVGEER---------GLK-------FIGKSLIASPKAEVLSMASETEEE 229
+ N +T +RVG E G K F G S +A+P + S + +
Sbjct: 297 IANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSRTPGLSRSRDG 356
Query: 230 VGVAEIDLYLVRNKRINDLNDIFKDRREEYYFR 262
+ VA++DL L +++ND+ + R E Y R
Sbjct: 357 LLVAKLDLNLC--QQVNDVWNFKMTGRYEMYAR 387
>pir||T48563 hypothetical protein F14F18.210 - Arabidopsis thaliana
>gi|7573371|emb|CAB87677.1| (AL163812) putative protein
[Arabidopsis thaliana]
Length = 318
Score = 93.6 bits (229), Expect = 2e-18
Identities = 84/277 (30%), Positives = 139/277 (49%), Gaps = 44/277 (15%)
Query: 18 KNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEI-AQKIPEG----ETTTFL 72
+N S A+K I+EA+ +GA+LV+LPE++++ Y+ + F + A++I G +T L
Sbjct: 41 RNISHAKKAIEEAASKGAKLVLLPEIWNSPYS----NDSFPVYAEEIDAGGDASPSTAML 96
Query: 73 MDVARDTGVYIVAGT-AEKDGDVLYNSAVVVGPRGFI-GKYRKIHLF---------YREK 121
+V++ + I+ G+ E+ GD LYN+ V G G + K+RKIHLF + E
Sbjct: 97 SEVSKRLKITIIGGSIPERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPGKITFMES 156
Query: 122 FFFEPG------DLGFR------VFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHP 169
G D G+ + D+G ++G+ IC+D F E A A +GA ++ +P
Sbjct: 157 KTLTAGETPTIVDTGYNLGLPNIIPDVG--RIGIGICYDIRFQELAMIYAARGAHLLCYP 214
Query: 170 ANLVMPYAPRAMPI----RALENKVYTVTADRVGEE-RGLKFIGKSLIASPKAEVLSMAS 224
M P + RA +N++Y T + G G S + P EVL+ +
Sbjct: 215 GAFNMTTGPLHWELLQRARATDNQLYVATCSPARDSGAGYTAWGHSTLVGPFGEVLA-TT 273
Query: 225 ETEEEVGVAEIDLYLVRNKRIN-DLNDIFKDRREEYY 260
E EE + +AEID ++ +R + LN + RR + Y
Sbjct: 274 EHEEAIIIAEIDYSILEQRRTSLPLN---RQRRGDLY 307
>gi|6323383 nitrilase superfamily member; Nit3p [Saccharomyces cerevisiae]
>gi|1730592|sp|P49954|YL85_YEAST HYPOTHETICAL 32.5 KD
PROTEIN YLR351C >gi|1077418|pir||S51459 hypothetical
protein YLR351c - yeast (Saccharomyces cerevisiae)
>gi|609401|gb|AAB67751.1| (U19102) Ylr351cp
[Saccharomyces cerevisiae]
>gi|9367112|gb|AAF87101.1|AF284572_1 (AF284572) Nit
protein 3 [Saccharomyces cerevisiae]
Length = 291
Score = 93.2 bits (228), Expect = 3e-18
Identities = 76/279 (27%), Positives = 131/279 (46%), Gaps = 24/279 (8%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQ--GAQLVVLPELFDTGYNFETREEVFEI 59
+KVA VQ++ + N +A I+ A K+ +LVVLPE F++ Y+ + + E+
Sbjct: 11 IKVALVQLSGSSPDKMANLQRAATFIERAMKEQPDTKLVVLPECFNSPYSTDQFRKYSEV 70
Query: 60 AQKIPEGETTTFLMDVARDTGVYIVAGTA---EKDGDVLYNSAVVVGPRG-FIGKYRKIH 115
+ FL ++A + +V GT + D +YN++++ G I K+RK+H
Sbjct: 71 INPKEPSTSVQFLSNLANKFKIILVGGTIPELDPKTDKIYNTSIIFNEDGKLIDKHRKVH 130
Query: 116 LF---------YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVI 166
LF + E PG+ + D + K GV IC+D FPE A A KGA +
Sbjct: 131 LFDVDIPNGISFHESETLSPGEKSTTI-DTKYGKFGVGICYDMRFPELAMLSARKGAFAM 189
Query: 167 AHPANLVMPYAPRAMPI----RALENKVYTVTADRVGE-ERGLKFIGKSLIASPKAEVLS 221
+P+ P + RA++N+VY + + G S++ P+ ++++
Sbjct: 190 IYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVDPRGKIVA 249
Query: 222 MASETEEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
A E EE + AE+D ++ + R + K RR + Y
Sbjct: 250 EAGEGEEII-YAELDPEVIESFR--QAVPLTKQRRFDVY 285
>dbj|BAB09868.1| (AB008268) beta-ureidopropionase [Arabidopsis thaliana]
Length = 405
Score = 92.8 bits (227), Expect = 4e-18
Identities = 71/251 (28%), Positives = 122/251 (48%), Gaps = 30/251 (11%)
Query: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVF-EIAQKIPEGETTTFLMDVARD 78
+ K + +I A G ++ L E + + F TRE + E A+ + +GE+T FL ++A+
Sbjct: 112 FDKLKPIIDAAGVAGVNILCLQEAWTMPFAFCTRERRWCEFAEPV-DGESTKFLQELAKK 170
Query: 79 TGVYIVAGTAEKD---GDVLYNSAVVVGPRG-FIGKYRKIHL----FYREKFFFEPGDLG 130
+ IV+ E+D G+VL+N+AV++G G IGK+RK H+ + E ++ GD G
Sbjct: 171 YNMVIVSPILERDIDHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTG 230
Query: 131 FRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPIR----AL 186
VF+ F K+ V IC+ P + L GA+++ +P+ V + PI A+
Sbjct: 231 HPVFETVFGKIAVNICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNAAI 290
Query: 187 ENKVYTVTADRVGEERGLK----------------FIGKSLIASPKAEVLSMASETEEEV 230
N + + +RVG E F G S ++P A S ++ +
Sbjct: 291 ANSYFVGSINRVGTEVFPNPFTSGDGKPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGL 350
Query: 231 GVAEIDLYLVR 241
++++DL L R
Sbjct: 351 LISDMDLNLCR 361
>sp|Q03248|BUP_RAT BETA-UREIDOPROPIONASE (BETA-ALANINE SYNTHASE)
(N-CARBAMOYL-BETA-ALANINE AMIDOHYDROLASE)
>gi|285064|pir||S27881 beta-alanine synthase - rat
>gi|203106|gb|AAA40804.1| (M97662) beta-alanine synthase
[Rattus norvegicus]
Length = 393
Score = 90.1 bits (220), Expect = 2e-17
Identities = 73/273 (26%), Positives = 129/273 (46%), Gaps = 32/273 (11%)
Query: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMDVAR 77
+ + E++ + A+ G ++ E ++ + F TRE++ E A+ +G TT F +A+
Sbjct: 97 HKRIEEIAEVAAMCGVNIICFQEAWNMPFAFCTREKLPWTEFAESAEDGLTTRFCQKLAK 156
Query: 78 DTGVYIVAGTAEKD---GDVLYNSAVVVGPRGFI-GKYRKIHL----FYREKFFFEPGDL 129
+ +++ E+D G VL+N+AVV+ G + GK RK H+ + E ++ G+L
Sbjct: 157 KHNMVVISPILERDRDHGGVLWNTAVVISNSGLVMGKTRKNHIPRVGDFNESTYYMEGNL 216
Query: 130 GFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPIR----A 185
G VF F ++ V IC+ P + ++ GA++I +P+ + + PI A
Sbjct: 217 GHPVFQTQFGRIAVNICYGRHHPLNWLMYSVNGAEIIFNPSATIGELSESMWPIEARNAA 276
Query: 186 LENKVYTVTADRVGEER---------GLK-------FIGKSLIASPKAEVLSMASETEEE 229
+ N +T +RVG+E G K F G S +A+P S ++
Sbjct: 277 IANHCFTCALNRVGQEHYPNEFTSGDGKKAHHDLGYFYGSSYVAAPDGSRTPGLSRNQDG 336
Query: 230 VGVAEIDLYLVRNKRINDLNDIFKDRREEYYFR 262
+ V E++L L ++IND R E Y R
Sbjct: 337 LLVTELNLNLC--QQINDFWTFKMTGRLEMYAR 367
>gb|AAF06739.1| (AF169560) beta-ureidopropionase [Homo sapiens]
Length = 387
Score = 89.3 bits (218), Expect = 4e-17
Identities = 74/276 (26%), Positives = 133/276 (47%), Gaps = 35/276 (12%)
Query: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMDVAR 77
+ + + +++ A+ G ++ E + + F TRE++ E A+ +G TT F +A+
Sbjct: 97 HRRIKAIVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAK 156
Query: 78 DTGVYIVAGTAEKD---GDVLYNSAVVVGPRGFI-GKYRKIHL----FYREKFFFEPGDL 129
+ + +V+ E+D GDVL+N+AVV+ G + GK RK H+ + E ++ G+L
Sbjct: 157 NHDMVVVSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNL 216
Query: 130 GFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPA---NLVMPYAPRAMPIR-- 184
G VF F ++ V IC+ P + ++ GA++I +P+ + + PI
Sbjct: 217 GHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALRSLSESLWPIEAR 276
Query: 185 --ALENKVYTVTADRVGEER---------GLK-------FIGKSLIASPKAEVLSMASET 226
A+ N +T +RVG E G K F G S +A+P + S +
Sbjct: 277 NAAIANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSRTPGLSRS 336
Query: 227 EEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYYFR 262
+ + VA++DL L +++ND+ + R E Y R
Sbjct: 337 RDGLLVAKLDLNLC--QQVNDVWNFKMTGRYEMYAR 370
>pir||T36488 probable hydrolase - Streptomyces coelicolor
>gi|5457242|emb|CAB46930.1| (AL096822) putative
hydrolase [Streptomyces coelicolor A3(2)]
Length = 264
Score = 87.8 bits (214), Expect = 1e-16
Identities = 74/227 (32%), Positives = 109/227 (47%), Gaps = 29/227 (12%)
Query: 22 KAEKLIKEASKQGAQLVVLPELFDTG-YNFETREEVFEIAQKIPEGETTTFLMDVARDTG 80
+A L++E + GA LVVLPEL+ TG + FE F+ A + G T + A D G
Sbjct: 22 RAAALVREQA--GADLVVLPELWTTGAFAFEE----FDAAAEPLRGPTYEAMAKAASDAG 75
Query: 81 VYIVAGTAEKDGDV--LYNSAVVVGPRGFI-GKYRKIHLFYREKFFFEPGDLGFR----- 132
V++ AG+ + G LYN+++V P G + YRKIH +F F+ G+
Sbjct: 76 VWLHAGSVPERGPDGRLYNTSLVFSPAGDLTASYRKIH-----RFGFDKGEAVLMGAGRE 130
Query: 133 --VFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRA-----MPIRA 185
L +GV C+D FPE R+L GA+++ PA P RA RA
Sbjct: 131 PVTVRLPDTTLGVATCYDLRFPELFRSLVDAGAEILVVPAG--WPERRRAHWTLLARARA 188
Query: 186 LENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGV 232
+EN+ + + G G+ G S++ P EVL+ A EE + V
Sbjct: 189 VENQAFVLACGTAGTHAGVPQAGHSIVVDPWGEVLAEAGADEEVLAV 235
>gb|AAC39137.1| (AF069989) nitrilase and fragile histidine triad fusion protein
NitFhit [Drosophila melanogaster]
>gi|7291930|gb|AAF47347.1| (AE003467) NitFhit gene
product [Drosophila melanogaster]
Length = 460
Score = 87.4 bits (213), Expect = 2e-16
Identities = 69/257 (26%), Positives = 127/257 (48%), Gaps = 20/257 (7%)
Query: 19 NYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDVARD 78
N S+ +L+ A Q A ++ LPE D + E+R + E+++ + +GE ++A+
Sbjct: 49 NLSQVIELVDRAKSQNACMLFLPECCD--FVGESRTQTIELSEGL-DGELMAQYRELAKC 105
Query: 79 TGVYI-VAGTAEKDGDVLYNSAVVVGPRGFIGK-YRKIHLF--------YREKFFFEPGD 128
++I + G E++ ++N+ V++ +G + YRK+H+F RE PG
Sbjct: 106 NKIWISLGGVHERNDQKIFNAHVLLNEKGELAAVYRKLHMFDVTTKEVRLRESDTVTPGY 165
Query: 129 LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPI----R 184
R ++G+ IC+D F E A L GA+++ +P+ I R
Sbjct: 166 CLERPVSTPVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAFTYATGKAHWEILLRAR 225
Query: 185 ALENKVYTVTADRVG-EERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRNK 243
A+E + + V A ++G + + G S+I SP VL+ SE E ++G AE+DL ++++
Sbjct: 226 AIETQCFVVAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQELDIGTAEVDLSVLQS- 284
Query: 244 RINDLNDIFKDRREEYY 260
+ F+ RR + Y
Sbjct: 285 -LYQTMPCFEHRRNDIY 300
>gb|AAF54141.1| (AE003676) CG3027 gene product [Drosophila melanogaster]
Length = 406
Score = 87.4 bits (213), Expect = 2e-16
Identities = 56/197 (28%), Positives = 103/197 (51%), Gaps = 14/197 (7%)
Query: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMDVAR 77
++K + +IK A++ G +V E + + F TRE+ E A++ G TT L ++A+
Sbjct: 98 WNKVKTMIKAAAEAGCNIVCTQEAWTMPFAFCTREKFPWCEFAEEAENGPTTKMLAELAK 157
Query: 78 DTGVYIVAGTAEKD---GDVLYNSAVVVGPRG-FIGKYRKIHL----FYREKFFFEPGDL 129
+ I+ E+D G+ ++N+AVV+ G ++GK+RK H+ + E ++ G+
Sbjct: 158 AYNMVIIHSILERDMEHGETIWNTAVVISNSGRYLGKHRKNHIPRVGDFNESTYYMEGNT 217
Query: 130 GFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPIR----A 185
G VF+ F K+ V IC+ P++ L GA+++ +P+ + + I A
Sbjct: 218 GHPVFETEFGKLAVNICYGRHHPQNWMMFGLNGAEIVFNPSATIGRLSEPLWSIEARNAA 277
Query: 186 LENKVYTVTADRVGEER 202
+ N +TV +RVG E+
Sbjct: 278 IANSYFTVPINRVGTEQ 294
>sp|Q9X0Y0|NAE2_THEMA PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
[GLUTAMINE-HYDROLYSING]) >gi|7451135|pir||G72277
NH(3)-dependent NAD(+) synthetase - Thermotoga maritima
(strain MSB8) >gi|4981808|gb|AAD36328.1|AE001780_12
(AE001780) NH(3)-dependent NAD(+) synthetase [Thermotoga
maritima]
Length = 576
Score = 86.6 bits (211), Expect = 3e-16
Identities = 75/250 (30%), Positives = 124/250 (49%), Gaps = 21/250 (8%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
++V Q+NP + + + N KA + ++ A +G+ L+V PELF GY E +
Sbjct: 4 LRVTLAQLNPTLGDFEGNLKKAIEALRVAEDRGSDLLVFPELFLPGYPPEDLMLRLSFLR 63
Query: 62 KIPEGETTTFLMDVA---RDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHL-- 116
E +L A R+ GV ++ G + D D YN+A VV +G YRKI L
Sbjct: 64 -----ENRKYLQKFAQHTRNLGVTVLMGFIDSDEDA-YNAAAVVKDGEILGVYRKISLPN 117
Query: 117 --FYREKFFFEPGDLGFRVFDLGFMKVGVMICFD-WFFPESARTLAL-KGADVIAH---- 168
+ E+ +F+PG+ V +G +KVGV IC D W E + +L+L +G +IA+
Sbjct: 118 YGVFDERRYFKPGE-ELLVVKIGNIKVGVTICEDIWNPVEPSASLSLGEGVHLIANLSAS 176
Query: 169 PANLVMPYAPR-AMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETE 227
P ++ P + + ++A + V + VG + L F G S++ EV++ E
Sbjct: 177 PYHVGKPVLRKDYLSMKAYDYHVAMAYCNMVGGQDELVFDGGSMVVDASGEVINYGKLFE 236
Query: 228 EEVGVAEIDL 237
EE+ ++DL
Sbjct: 237 EEIITVDLDL 246
>gb|AAC38297.1| (AF012132) unknown [Staphylococcus epidermidis]
Length = 264
Score = 83.9 bits (204), Expect = 2e-15
Identities = 70/268 (26%), Positives = 128/268 (47%), Gaps = 15/268 (5%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
++K+ +Q N + DKN + + + +VVLPE+++ GY E E+ +
Sbjct: 3 ILKIQILQFNVERGNVDKNMQNIKTKFNQYLDKDTSVVVLPEMWNNGYALEELEQKADKN 62
Query: 61 QKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDV-LYNSAVVVGP-RGFIGKYRKIHL-- 116
K +++ F+ D+A V I+AG+ + +YN+A + + I +Y K+HL
Sbjct: 63 LK----DSSLFIKDLAHTFNVDIIAGSVSNIRENHIYNTAFAINKNKELINEYDKVHLVP 118
Query: 117 FYREKFFFEPGDLGFRVFDLGFMK-VGVMICFDWFFPESARTLALKGADV---IAHPANL 172
RE F G++ F L V +IC+D FPE R A KGA + +A +
Sbjct: 119 MLREPDFLCGGNVVPEPFYLSDQTLVTQIICYDLRFPEILRYPARKGAKIAFYVAQWPSS 178
Query: 173 VMPYAPRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGV 232
+ + + RA+EN ++ V + G++ + G S++ +P E+L + +E V
Sbjct: 179 RLDHWLSLLKARAIENDIFIVACNSCGDDGHTNYAGNSIVINPNGEILDHLDD-KEGVLT 237
Query: 233 AEIDLYLVRNKRINDLNDIFKDRREEYY 260
ID+ LV +R + +F++ + Y
Sbjct: 238 THIDVDLVDQQR--EYIPVFRNLKPHLY 263
>gb|AAF87104.1|AF284575_1 (AF284575) Nit protein 1 [Xenopus laevis]
Length = 288
Score = 83.1 bits (202), Expect = 3e-15
Identities = 73/269 (27%), Positives = 127/269 (47%), Gaps = 31/269 (11%)
Query: 15 EPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMD 74
+ +KN++ +LI+EA+ + A +V LPE FD Y + EE +A+ + G+T
Sbjct: 19 DKEKNFATCSRLIREAAGRRACMVFLPEAFD--YIGGSIEETLSLAESL-HGDTIQRYTQ 75
Query: 75 VARDTGVYIVAGTAEKDG------DVLYNSAVVVGPRGFI-GKYRKIHLF---------Y 118
+AR+ G+++ G + G + NS VVV G I YRK HLF
Sbjct: 76 LARECGLWLSLGGFHEKGPNWDTDQRISNSHVVVDNTGHIVSVYRKAHLFDVDLQNGVSL 135
Query: 119 REKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLV----M 174
RE PG R K+G+ +C+D FPE + LA +GA+++ +P+ +
Sbjct: 136 RESSSTLPGAELIRPITSPAGKIGLGVCYDLRFPEFSLALAQQGAELLTYPSAFTLTTGL 195
Query: 175 PYAPRAMPIRALENKVYTVTA---DRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVG 231
+ + RA+E + Y V A DR E+R G +++ P V+ + +E G
Sbjct: 196 AHWEVLLRARAIETQCYVVAAAQTDRHNEKR--TSYGHAMVVDPWGLVI---GQCQEGTG 250
Query: 232 VAEIDLYLVRNKRINDLNDIFKDRREEYY 260
+ ++ + +R+ +++ RR + Y
Sbjct: 251 ICYAEIDIPYMERVRRDMPVWRHRRTDLY 279
>sp|Q10166|YAUB_SCHPO HYPOTHETICAL 35.7 KD PROTEIN C26A3.11 IN CHROMOSOME I
>gi|7492388|pir||T38399 probable amidohydrolase -
fission yeast (Schizosaccharomyces pombe)
>gi|1177358|emb|CAA93234.1| (Z69240) putative
amidohydrolase [Schizosaccharomyces pombe]
Length = 322
Score = 82.7 bits (201), Expect = 4e-15
Identities = 75/264 (28%), Positives = 121/264 (45%), Gaps = 26/264 (9%)
Query: 15 EPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFE-IAQKIPEGETTTFLM 73
+ +N A + EA+K G+ ++VLPE+F++ Y + E I + P + L
Sbjct: 56 DKSENLQLARLKVLEAAKNGSNVIVLPEIFNSPYGTGYFNQYAEPIEESSPSYQA---LS 112
Query: 74 DVARDTGVYIVAGT--AEKDGDVLYNSAVVVGPRG-FIGKYRKIHLF---------YREK 121
+A+DT Y+ G+ KDG LYN+A+V P G I +RKIHLF +RE
Sbjct: 113 SMAKDTKTYLFGGSIPERKDGK-LYNTAMVFDPSGKLIAVHRKIHLFDIDIPGGVSFRES 171
Query: 122 FFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAM 181
PGD + D + K G+ IC+D FPE A A G V+ +P + P
Sbjct: 172 DSLSPGD-AMTMVDTEYGKFGLGICYDIRFPELAMIAARNGCSVMIYPGAFNLSTGPLHW 230
Query: 182 PI----RALENKVYTV-TADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEID 236
+ RA++N+++ A G S + P +V++ E + + A+ID
Sbjct: 231 ELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVVDPFGKVIATTDE-KPSIVYADID 289
Query: 237 LYLVRNKRINDLNDIFKDRREEYY 260
++ R + I+ RR + Y
Sbjct: 290 PSVMSTAR--NSVPIYTQRRFDVY 311
>gb|AAK40748.1| Conserved hypothetical protein [Sulfolobus solfataricus]
Length = 293
Score = 81.5 bits (198), Expect = 9e-15
Identities = 70/240 (29%), Positives = 120/240 (49%), Gaps = 20/240 (8%)
Query: 4 VAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFE-------TREEV 56
+A V + + L N KA+KLI++A +GA+LV+LP LF +G FE R +
Sbjct: 8 IALVHLRLKELSRKHNLEKAKKLIRQAKDKGAKLVILPSLFPSGNMFEIYDNDKKLRSFI 67
Query: 57 FEIAQKIPEGETTTFLMDVARDTGVYIVAG-TAEKDGDVLYNSAVVVGPRG-FIGKYRKI 114
+A+KIP G T L+++A D V+++ G E+ G ++ +++++ P+G IGKYRK
Sbjct: 68 KNLAEKIP-GNNTDTLINLAMDGEVHVIVGPILEQAGPKIFLTSLIISPQGEIIGKYRKT 126
Query: 115 HLFYRE-KFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLV 173
L ++ + G V + K G++ D F PE +R LA+ G++++ +
Sbjct: 127 VLSEKDIRLGISAGKEPVNV--VLDRKYGIIAEDDIFSPEISRLLAMGGSEIVIGTMKAL 184
Query: 174 ---MPYAPRAMPIRALENKV-YTVTADRVGEERGLKFIGKS--LIASPKAEVLSMASETE 227
R +EN + Y + + + +E G + IG S I SP + A E +
Sbjct: 185 GKEQQVIKHLAIARTIENGIPYLIVGESIEDEEG-EIIGYSPTFITSPDNLISKEAEEDD 243
>pir||A72510 hypothetical protein APE2055 - Aeropyrum pernix (strain K1)
>gi|5105753|dbj|BAA81065.1| (AP000063) 302aa long
hypothetical protein [Aeropyrum pernix]
Length = 302
Score = 81.1 bits (197), Expect = 1e-14
Identities = 60/175 (34%), Positives = 89/175 (50%), Gaps = 26/175 (14%)
Query: 2 VKVAYVQMNPQILEPDK--NYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEI 59
++VA VQ P +P N KA L+ AS+ +VV PE F
Sbjct: 8 LRVAVVQPAPVNNDPSHPGNPLKAAGLVTRASRLDPDIVVFPEYF--------------- 52
Query: 60 AQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGF-IGKYRKIHL-- 116
P E+ FL D D+GVY+VAG A ++G LYN+A + P G+ + + RK ++
Sbjct: 53 ----PFHESRGFL-DAVADSGVYVVAGIAYREGGRLYNTATIYSPEGYPLYRQRKRYVGR 107
Query: 117 FYREKFFFEPGDLGFRVFDLGFMKVGVMICFD-WFFPESARTLALKGADVIAHPA 170
R + F+ + V D+G +K+GV +C D W FPE+A L L GAD+ +P+
Sbjct: 108 LERRLWGFDRWSGDYGVVDIGGVKLGVAVCADFWSFPEAALELFLGGADLFINPS 162
>sp|P54608|YHCX_BACSU HYPOTHETICAL 60.2 KD PROTEIN IN CSPB-GLPP INTERGENIC REGION
>gi|7474587|pir||D69824 conserved hypothetical protein
yhcX - Bacillus subtilis >gi|1240000|emb|CAA65708.1|
(X96983) hypothetical protein [Bacillus subtilis]
>gi|2633248|emb|CAB12753.1| (Z99108) similar to
hypothetical proteins [Bacillus subtilis]
Length = 513
Score = 80.8 bits (196), Expect = 2e-14
Identities = 77/264 (29%), Positives = 122/264 (46%), Gaps = 29/264 (10%)
Query: 21 SKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA-QKIPE--GETTTFLMDVAR 77
++ E + AS + V PE+F T E +A Q+I E + + D+A
Sbjct: 249 NQVEYYVDVASDARSDFAVFPEIFTTQLMSFLEERSPSLAVQRITEYTEDYISLFTDLAV 308
Query: 78 DTGVYIVAGT--AEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYREKFFF--EPGDLGFRV 133
V I+ G+ E++G + YN A + G I K K+H+ E+ ++ GD RV
Sbjct: 309 KYNVNIIGGSHFVEEEGKI-YNIAYLFRRDGTIEKQYKLHITPNERKWWGISAGDQ-VRV 366
Query: 134 FDLGFMKVGVMICFDWFFPESARTLALKGADVIAHP-------ANLVMPYAPRAMPIRAL 186
FD K+ + IC+D FPE AR A KGA +I P L + Y +A RA+
Sbjct: 367 FDTDCGKIAIQICYDIEFPELARIAADKGAKIIFTPFCTEDRQGYLRVRYCSQA---RAV 423
Query: 187 ENKVYTVTADRVG-----EERGLKFIGKSLIASPKAE-----VLSMASETEEEVGVAEID 236
EN++YTV + VG E +++ + A E ++ + E V + ++D
Sbjct: 424 ENQIYTVISGTVGNLPQTENMDIQYAQSGIFAPSDFEFARDGIVGETNPNIEMVVIGDVD 483
Query: 237 LYLVRNKRINDLNDIFKDRREEYY 260
L ++R +R N KDRR + Y
Sbjct: 484 LEILRRQRQNGTVRQLKDRRRDIY 507
>gi|6754856 nitrilase 1 [Mus musculus] >gi|3228668|gb|AAC40185.1| (AF069988)
nitrilase 1 [Mus musculus]
Length = 323
Score = 80.8 bits (196), Expect = 2e-14
Identities = 75/282 (26%), Positives = 125/282 (43%), Gaps = 32/282 (11%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
+V V V P E N+ +L++EA++ GA L LPE FD F R +
Sbjct: 44 LVAVCQVTSTPNKQE---NFKTCAELVQEAARLGACLAFLPEAFD----FIARNPAETLL 96
Query: 61 QKIP-EGETTTFLMDVARDTGVYIVAGTAEKDGD------VLYNSAVVVGPRG-FIGKYR 112
P G+ +AR+ G+++ G + G +YN V++ +G + YR
Sbjct: 97 LSEPLNGDLLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYR 156
Query: 113 KIHLF---------YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
K HL RE + +PG KVG+ IC+D FPE + LA GA
Sbjct: 157 KTHLCDVEIPGQGPMRESNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPELSLKLAQAGA 216
Query: 164 DVIAHPANLVMPYAPRAMPI----RALENKVYTVTADRVGEERGLK-FIGKSLIASPKAE 218
+++ +P+ P + RA+E++ Y + A + G + G S++ P
Sbjct: 217 EILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGT 276
Query: 219 VLSMASETEEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
V++ SE + +A IDL+ ++ R + +F+ RR + Y
Sbjct: 277 VVARCSE-GPGLCLARIDLHFLQQMRQH--LPVFQHRRPDLY 315
>gb|AAC40184.1| (AF069985) nitrilase homolog 1 [Mus musculus]
Length = 323
Score = 80.8 bits (196), Expect = 2e-14
Identities = 75/282 (26%), Positives = 125/282 (43%), Gaps = 32/282 (11%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
+V V V P E N+ +L++EA++ GA L LPE FD F R +
Sbjct: 44 LVAVCQVTSTPNKQE---NFKTCAELVQEAARLGACLAFLPEAFD----FIARNPAETLL 96
Query: 61 QKIP-EGETTTFLMDVARDTGVYIVAGTAEKDGD------VLYNSAVVVGPRG-FIGKYR 112
P G+ +AR+ G+++ G + G +YN V++ +G + YR
Sbjct: 97 LSEPLNGDLLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYR 156
Query: 113 KIHLF---------YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
K HL RE + +PG KVG+ IC+D FPE + LA GA
Sbjct: 157 KTHLCDVEIPGQGPMRESNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPELSLKLAQAGA 216
Query: 164 DVIAHPANLVMPYAPRAMPI----RALENKVYTVTADRVGEERGLK-FIGKSLIASPKAE 218
+++ +P+ P + RA+E++ Y + A + G + G S++ P
Sbjct: 217 EILTYPSAFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGT 276
Query: 219 VLSMASETEEEVGVAEIDLYLVRNKRINDLNDIFKDRREEYY 260
V++ SE + +A IDL+ ++ R + +F+ RR + Y
Sbjct: 277 VVARCSE-GPGLCLARIDLHFLQQMRQH--LPVFQHRRPDLY 315
>pir||A70310 conserved hypothetical protein aq_103 - Aquifex aeolicus
>gi|2982848|gb|AAC06470.1| (AE000673) hypothetical
protein [Aquifex aeolicus]
Length = 246
Score = 80.4 bits (195), Expect = 2e-14
Identities = 68/228 (29%), Positives = 112/228 (48%), Gaps = 18/228 (7%)
Query: 16 PDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDV 75
P++N K +++ + LV+LPE++ +G+++E EE AQK PE L +
Sbjct: 13 PEENLKKVINFLEKVEENS--LVLLPEMWYSGFDYENLEEH---AQKTPE--VLEVLKKI 65
Query: 76 ARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLF--YREKFFFEPGDLGFRV 133
+++ + + EK + + N+A ++ IGK KI LF + E +F PG +V
Sbjct: 66 SKEKSLTLCGTLPEKGTEGILNTAFLIEDGRVIGKRSKIKLFPIFDEDKYFIPGKEN-KV 124
Query: 134 FDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRA-----MPIRALEN 188
F+ K G++ICF+ F + + DV+ PA YA R RA+E
Sbjct: 125 FETKLGKAGILICFEIRFTDLIMNFWRERPDVVLVPAQ--WGYARRKHFETLCRARAIEL 182
Query: 189 KVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEID 236
+ Y + ++ GE G +F G S I SP EVL+ SE + + A+ D
Sbjct: 183 QAYLLASNTWGEYLGTRFAGHSGIYSPWGEVLAF-SEKGDTLLAADYD 229
>sp|P46010|NRL3_ARATH NITRILASE 3 >gi|11266291|pir||T49148 nitrilase 3 - Arabidopsis
thaliana >gi|508735|gb|AAA19627.1| (U09959) nitrilase
[Arabidopsis thaliana] >gi|6580144|emb|CAA68936.2|
(Y07648) nitrilase 3 [Arabidopsis thaliana]
>gi|7649383|emb|CAB89000.1| (AL353865) nitrilase 3
[Arabidopsis thaliana]
Length = 346
Score = 79.2 bits (192), Expect = 5e-14
Identities = 78/275 (28%), Positives = 122/275 (44%), Gaps = 41/275 (14%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------- 48
V+V VQ + + KAEK I EA+ +GA+LV+ PE F GY
Sbjct: 25 VRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAVGVH 84
Query: 49 NFETREE---VFEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPR 105
N E R+E A K+P G L ++A V++V G EKDG LY +A+ P+
Sbjct: 85 NEEGRDEFRNYHASAIKVP-GPEVERLAELAGKNNVHLVMGAIEKDGYTLYCTALFFSPQ 143
Query: 106 G-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
G F+GK+RK+ E+ + GD V+D K+G IC++ P L KG
Sbjct: 144 GQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYAKGI 203
Query: 164 DVIAHP-ANLVMPYAPRAMPIRALENKVYTVTADRVGEERGLK----------------- 205
++ P A+ + + + I A+E + ++A + + R
Sbjct: 204 EIYCAPTADYSLEWQASMIHI-AVEGGCFVLSAHQFCKRREFPEHPDYLFNDIVDTKEHD 262
Query: 206 ---FIGKSLIASPKAEVLSMASETEEEVGVAEIDL 237
G S+I SP +VL+ + E + A++DL
Sbjct: 263 PTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDL 297
>sp|P32961|NRL1_ARATH NITRILASE 1 >gi|99738|pir||S22398 nitrilase (EC 3.5.5.1) -
Arabidopsis thaliana >gi|16400|emb|CAA45041.1| (X63445)
nitrilase I [Arabidopsis thaliana]
Length = 346
Score = 78.8 bits (191), Expect = 6e-14
Identities = 79/274 (28%), Positives = 118/274 (42%), Gaps = 39/274 (14%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------- 48
V+V VQ + + KAEK I EA+ +GA+LV+ PE F GY
Sbjct: 25 VRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVH 84
Query: 49 NFETREEVFEI---AQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPR 105
N E R+E + A +P G L DVAR VY+V G EK+G LY + + P+
Sbjct: 85 NEEGRDEFRKYHASAIHVP-GPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143
Query: 106 G-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
G F+GK+RK+ E+ + GD V+D K+G IC++ P L KG
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203
Query: 164 DVIAHPANLVMPYAPRAMPIRALENKVYTVTADRV--------------------GEERG 203
++ P +M A+E + ++A + E
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263
Query: 204 LKFIGKSLIASPKAEVLSMASETEEEVGVAEIDL 237
+ G S+I SP +VL+ + E + A+IDL
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDL 297
>pir||T49147 nitrilase 1 - Arabidopsis thaliana >gi|1389699|gb|AAB05221.1|
(U38845) nitrilase 1 [Arabidopsis thaliana]
>gi|6580143|emb|CAA68935.2| (Y07648) nitrilase 1
[Arabidopsis thaliana] >gi|7649382|emb|CAB88999.1|
(AL353865) nitrilase 1 [Arabidopsis thaliana]
Length = 346
Score = 78.8 bits (191), Expect = 6e-14
Identities = 79/274 (28%), Positives = 118/274 (42%), Gaps = 39/274 (14%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------- 48
V+V VQ + + KAEK I EA+ +GA+LV+ PE F GY
Sbjct: 25 VRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVH 84
Query: 49 NFETREEVFEI---AQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPR 105
N E R+E + A +P G L DVAR VY+V G EK+G LY + + P+
Sbjct: 85 NEEGRDEFRKYHASAIHVP-GPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143
Query: 106 G-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
G F+GK+RK+ E+ + GD V+D K+G IC++ P L KG
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203
Query: 164 DVIAHPANLVMPYAPRAMPIRALENKVYTVTADRV--------------------GEERG 203
++ P +M A+E + ++A + E
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263
Query: 204 LKFIGKSLIASPKAEVLSMASETEEEVGVAEIDL 237
+ G S+I SP +VL+ + E + A+IDL
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDL 297
>sp|P55178|YAG5_STALU HYPOTHETICAL PROTEIN IN AGR OPERON (ORF 5)
>gi|295136|gb|AAA71975.1| (L13334) ORF5 [Staphylococcus
lugdunensis]
Length = 234
Score = 78.8 bits (191), Expect = 6e-14
Identities = 64/216 (29%), Positives = 107/216 (48%), Gaps = 13/216 (6%)
Query: 37 LVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDVARDTGVYIVAGT-AEKDGDVL 95
+V+LPE+++ GY E EE + + +T F+ ++A V I+AG+ + K D +
Sbjct: 9 VVILPEMWNNGYALEQLEEKADFDLE----RSTDFIKNLALQYQVDIIAGSVSNKHHDHI 64
Query: 96 YNSAVVVGPRG-FIGKYRKIHL--FYREKFFFEPGDLGFRVFDLGF-MKVGVMICFDWFF 151
+N+A + G I +Y K+HL E F G F L +KV MIC+D F
Sbjct: 65 FNTAFAIDKTGKVINQYDKMHLVPMLDEPAFLTAGKNVPETFKLSNGVKVTQMICYDLRF 124
Query: 152 PESARTLALKGADV---IAHPANLVMPYAPRAMPIRALENKVYTVTADRVGEERGLKFIG 208
PE R A GA + +A + + + + RA+EN +Y + + G + ++ G
Sbjct: 125 PELLRYPARSGATIAFYVAQWPSARLNHWQVLLKARAIENNMYVIGCNGCGYDGKTQYAG 184
Query: 209 KSLIASPKAEVLSMASETEEEVGVAEIDLYLVRNKR 244
S+ +P E++ S TE+E+ V ID+ V +R
Sbjct: 185 HSVAINPNGEIIQELSTTEKELTVT-IDIDAVEQQR 219
>emb|CAA72932.1| (Y12252) AmiE protein, aliphatic amidase [Helicobacter pylori]
Length = 339
Score = 78.0 bits (189), Expect = 1e-13
Identities = 68/260 (26%), Positives = 119/260 (45%), Gaps = 12/260 (4%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQK 62
K+ + Q+LE +N +K +K+ G L++ PE G ++ R+E+F+ A
Sbjct: 21 KMPRLHTKEQVLENCRNIAKVIGGVKQGLP-GLDLIIFPEYSTHGIMYD-RQEMFDTAAS 78
Query: 63 IPEGETTTFLMDVARDTGVYIVAGTAEKDGDVL---YNSAVVVGPRG-FIGKYRKIHLFY 118
+P ET F ++ + + T EK YN+ ++V +G + KYRKI +
Sbjct: 79 VPGEETAIFAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWC 138
Query: 119 REKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAP 178
+ ++ PGD + V +KV ++IC D +PE R A++GA++I + P
Sbjct: 139 PIECWY-PGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKE 197
Query: 179 RAMPI---RALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEI 235
+ + I A N+ Y A+ G + + G S I L E E + A++
Sbjct: 198 QQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGHTLGECGEEENGLQYAQL 257
Query: 236 DLYLVRNKRIND--LNDIFK 253
+ +R+ R D N +FK
Sbjct: 258 SVQQIRDARKYDQSQNQLFK 277
>pir||B71951 aliphatic amidase - Helicobacter pylori (strain J99)
>gi|4154804|gb|AAD05860.1| (AE001465) ALIPHATIC AMIDASE
[Helicobacter pylori J99]
Length = 339
Score = 78.0 bits (189), Expect = 1e-13
Identities = 69/261 (26%), Positives = 122/261 (46%), Gaps = 14/261 (5%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQK 62
K+ + ++LE +N +K +K+ G L++ PE G ++ R+E+F+ A
Sbjct: 21 KMPRLHTKNEVLENCRNIAKVIGGVKQGLP-GLDLIIFPEYSTHGIMYD-RQEMFDTAAS 78
Query: 63 IPEGETTTFLMDVARDTGVY-IVAGTAEKDGDVL---YNSAVVVGPRG-FIGKYRKIHLF 117
+P GE T L + + V+ + + T EK YN+ ++V +G + KYRKI +
Sbjct: 79 VP-GEETAILAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPW 137
Query: 118 YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYA 177
+ ++ PGD + V +KV ++IC D +PE R A++GA++I + P
Sbjct: 138 CPIECWY-PGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAK 196
Query: 178 PRAMPI---RALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAE 234
+ + I A N+ Y A+ G + + G S I L E E + A+
Sbjct: 197 EQQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGHTLGECGEEENGLQYAQ 256
Query: 235 IDLYLVRNKRIND--LNDIFK 253
+ + +R+ R D N +FK
Sbjct: 257 LSVQQIRDARKYDQSQNQLFK 277
>gi|11499237 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7483155|pir||F69455 conserved hypothetical protein
AF1647 - Archaeoglobus fulgidus
>gi|2648903|gb|AAB89595.1| (AE000989) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 258
Score = 77.6 bits (188), Expect = 1e-13
Identities = 67/240 (27%), Positives = 112/240 (45%), Gaps = 27/240 (11%)
Query: 19 NYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDVARD 78
++ AE+L A + GA+L++ PE F +A ++ + ET +FL ++ +
Sbjct: 13 DFGSAERLAMAAVEGGAELLLFPEYFSYS----------RLAPEVLD-ETLSFLKRISSE 61
Query: 79 TGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYREKFFFEPGDLGFRVFDLGF 138
GV +V+G A D + N + + IG KIH E+ +VF++
Sbjct: 62 HGV-VVSGNAVVDENGYRNRSFLFDSGELIGVQDKIHPTRVERELGIQCGTKLQVFEVRG 120
Query: 139 MKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMP----------IRALEN 188
+ ++C D +PE R ALKGA+++ +P V+ + +P R+ +N
Sbjct: 121 AMISTLVCADILYPEICRVAALKGAEIVLNP---VVSFRKSELPGEEYRYCLYFTRSFDN 177
Query: 189 KVYTVTADRVGEE-RGLKFIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRN-KRIN 246
V A VG G + +G+SLIAS + E EEV +A++DL +R K IN
Sbjct: 178 AYAIVKAGGVGTTFTGSEAVGRSLIASFDGILARFRDENAEEVVIADVDLKRIREYKTIN 237
>gb|AAF14257.1| (AF136599) aliphatic amidase [Bacillus stearothermophilus]
Length = 348
Score = 77.3 bits (187), Expect = 2e-13
Identities = 72/261 (27%), Positives = 118/261 (44%), Gaps = 13/261 (4%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQK 62
K+ + +++E KN + +K+ G LV+ PE G ++ R+E+FE A
Sbjct: 21 KMPRLHTKKEVIENAKNIANMIVGMKQGLP-GMDLVIFPEYSTMGIMYD-RKEMFETATT 78
Query: 63 IPEGETTTFLMDV--ARDTGVYIVAGTAEKDGDVL--YNSAVVVGPRG-FIGKYRKIHLF 117
IP ET F A GV+ + G ++ YN+ V++ +G + KYRKI +
Sbjct: 79 IPGPETEIFAEACRKANTWGVFSLTGEQHEEHPHKNPYNTLVLINNKGEIVQKYRKIIPW 138
Query: 118 YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYA 177
+ ++ PGD + +K+ ++IC D +PE R A+KGA++I + P
Sbjct: 139 CPIEGWY-PGDTTYVTEGPKGIKISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAK 197
Query: 178 PRAMPI---RALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAE 234
+ + + A N VY A+ G + + G S I L E E + AE
Sbjct: 198 EQQIMMAKTMAWANNVYVAVANATGFDGVYSYFGHSAIIGFDGRTLGECGEEENGIQYAE 257
Query: 235 IDLYLVRNKRIN--DLNDIFK 253
I L +R+ R N N +FK
Sbjct: 258 ISLSQIRDFRQNAQSQNHLFK 278
>pir||T27679 probable nitrilase (EC 3.5.5.1) ZK1058.6 - Caenorhabditis elegans
>gi|3881500|emb|CAA84681.1| (Z35604) contains similarity
to Pfam domain: PF00795 (Carbon-nitrogen hydrolase),
Score=416.5, E-value=7.9e-122, N=1 [Caenorhabditis
elegans]
Length = 305
Score = 76.9 bits (186), Expect = 2e-13
Identities = 71/269 (26%), Positives = 108/269 (39%), Gaps = 33/269 (12%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------ 48
M K+A VQ + + K +K ++EA+ GA+LV+ PE F GY
Sbjct: 1 MPKIAIVQAGTPLFDKPATLEKVKKNVEEAAGNGAELVLFPEAFIGGYPKWNSFGITMGT 60
Query: 49 -NFETREEVFEIAQKIPE--GETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPR 105
E R+E + E GE + + +A ++IV G E++ LY S P
Sbjct: 61 RTPEGRKEFKRYFENAIEENGEESKLIESLAAQNNIHIVIGVVEREASTLYCSVFFYSPS 120
Query: 106 GFIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGAD 164
G++GK+RK+ E+ + GD VF K+G IC++ + P TL K
Sbjct: 121 GYLGKHRKLLPTALERCVWGQGDGSTMPVFSTSVGKIGSAICWENYMPLYRMTLYSKEIQ 180
Query: 165 VIAHPANLVMPYAPRAMPIRALENKVYTVTADRV----------------GEERGLKFIG 208
+ P M ALE + + V+A + GE++ L G
Sbjct: 181 IYLAPTVDDRDVWLSTMRTIALEGRCFVVSACQFLKSSDYPLDHPLRKEHGEDKVL-IRG 239
Query: 209 KSLIASPKAEVLSMASETEEEVGVAEIDL 237
S P VL T+E + E DL
Sbjct: 240 GSCAVDPLGTVLVEPDFTKETIRYTEFDL 268
>pir||F64556 aliphatic amidase - Helicobacter pylori (strain 26695)
>gi|2313392|gb|AAD07363.1| (AE000548) aliphatic amidase
(aimE) [Helicobacter pylori 26695]
Length = 339
Score = 76.5 bits (185), Expect = 3e-13
Identities = 67/260 (25%), Positives = 119/260 (45%), Gaps = 12/260 (4%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQK 62
K+ + ++LE +N +K +K+ G L++ PE G ++ R+E+F+ A
Sbjct: 21 KMPRLHTKNEVLENCRNIAKVIGGVKQGLP-GLDLIIFPEYSTHGIMYD-RQEMFDTAAS 78
Query: 63 IPEGETTTFLMDVARDTGVYIVAGTAEKDGDVL---YNSAVVVGPRG-FIGKYRKIHLFY 118
+P ET F ++ + + T EK YN+ ++V +G + KYRKI +
Sbjct: 79 VPGEETAIFAEACKKNKVWGVFSLTGEKHEQAKKNPYNTLILVNDKGEIVQKYRKILPWC 138
Query: 119 REKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAP 178
+ ++ PGD + V +KV ++IC D +PE R A++GA++I + P
Sbjct: 139 PIECWY-PGDKTYVVDGPKGLKVSLIICDDGNYPEIWRDCAMRGAELIVRCQGYMYPAKE 197
Query: 179 RAMPI---RALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVAEI 235
+ + I A N+ Y A+ G + + G S I L E E + A++
Sbjct: 198 QQIAIVKAMAWANQCYVAVANATGFDGVYSYFGHSSIIGFDGHTLGECGEEENGLQYAQL 257
Query: 236 DLYLVRNKRIND--LNDIFK 253
+ +R+ R D N +FK
Sbjct: 258 SVQQIRDARKYDQSQNQLFK 277
>sp|P32962|NRL2_ARATH NITRILASE 2 >gi|322548|pir||S31969 nitrilase (EC 3.5.5.1)
[similarity] - Arabidopsis thaliana
>gi|22656|emb|CAA48377.1| (X68305) nitrilase II
[Arabidopsis thaliana] >gi|508733|gb|AAB60275.1|
(U09958) nitrilase [Arabidopsis thaliana]
>gi|7649381|emb|CAB88998.1| (AL353865) nitrilase 2
[Arabidopsis thaliana]
Length = 339
Score = 76.1 bits (184), Expect = 4e-13
Identities = 60/187 (32%), Positives = 88/187 (46%), Gaps = 19/187 (10%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------ 48
+V+ VQ + + KA K I EA+ +G++LVV PE F GY
Sbjct: 17 IVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGV 76
Query: 49 -NFETREEVFEI---AQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGP 104
N E R+E + A K+P G L ++A VY+V G EKDG LY +A+ P
Sbjct: 77 HNEEGRDEFRKYHASAIKVP-GPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSP 135
Query: 105 RG-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKG 162
+G F+GK+RK+ E+ + GD V+D K+G IC++ P L KG
Sbjct: 136 QGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKG 195
Query: 163 ADVIAHP 169
++ P
Sbjct: 196 IELYCAP 202
>emb|CAA68934.3| (Y07648) nitrilase 2 [Arabidopsis thaliana]
Length = 339
Score = 76.1 bits (184), Expect = 4e-13
Identities = 60/187 (32%), Positives = 88/187 (46%), Gaps = 19/187 (10%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------ 48
+V+ VQ + + KA K I EA+ +G++LVV PE F GY
Sbjct: 17 IVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGV 76
Query: 49 -NFETREEVFEI---AQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGP 104
N E R+E + A K+P G L ++A VY+V G EKDG LY +A+ P
Sbjct: 77 HNEEGRDEFRKYHASAIKVP-GPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSP 135
Query: 105 RG-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKG 162
+G F+GK+RK+ E+ + GD V+D K+G IC++ P L KG
Sbjct: 136 QGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKG 195
Query: 163 ADVIAHP 169
++ P
Sbjct: 196 IELYCAP 202
>gb|AAB05220.1| (U38845) nitrilase 2 [Arabidopsis thaliana]
Length = 339
Score = 75.7 bits (183), Expect = 5e-13
Identities = 60/187 (32%), Positives = 88/187 (46%), Gaps = 19/187 (10%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------ 48
+V+ VQ + + KA K I EA+ +G++LVV PE F GY
Sbjct: 17 IVRATIVQASTVYNDTPATLGKANKFIVEAATKGSELVVFPEAFIGGYPRGFRFGLGVGV 76
Query: 49 -NFETREEVFEI---AQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGP 104
N E R+E + A K+P G L ++A VY+V G EKDG LY +A+ P
Sbjct: 77 HNEEGRDEFRKYHASAIKVP-GPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSP 135
Query: 105 RG-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKG 162
+G F+GK+RK+ E+ + GD V+D K+G IC++ P L KG
Sbjct: 136 QGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKG 195
Query: 163 ADVIAHP 169
++ P
Sbjct: 196 IELYCAP 202
>dbj|BAA32602.1| (AB017194) hypothetical protein [Plectonema boryanum]
Length = 270
Score = 75.7 bits (183), Expect = 5e-13
Identities = 77/252 (30%), Positives = 121/252 (47%), Gaps = 30/252 (11%)
Query: 5 AYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETR-EEVFEIAQKI 63
A VQMN + + KN ++AE+LI+ A ++GA+L+ LPE F + E + + EIA
Sbjct: 7 AAVQMN-SLPDLQKNLAQAEELIEIAVRRGAELIGLPENFSFLGDEEGKIAQADEIAV-- 63
Query: 64 PEGETTTFLMDVARDTGVYIVAG--TAEKDGDVLYNSAVVVGPRG-FIGKYRKIHLF--- 117
E+ FL +A+ V I+ G + +YN+A++V P G + +Y K+HLF
Sbjct: 64 ---ESEKFLKTMAQRFQVTILGGGYPVPVEPGKVYNTALLVAPNGEELARYEKVHLFDVN 120
Query: 118 ------YREKFFFEPGDLGFRVF---DLGFMKVGVMICFDWFFPESARTLALKGADVIAH 168
YRE G + DLG +G+ +C+D FPE R + GA+V+
Sbjct: 121 LPDGNTYRESATVVAGTRVPPIHPSKDLG--NIGLSVCYDVRFPELYRQMTQLGAEVLFV 178
Query: 169 PANLV----MPYAPRAMPIRALENKVYTVTADRVGEERGLK-FIGKSLIASPKAEVLSMA 223
PA + + RA+EN Y + + G L+ G ++I P +L+ A
Sbjct: 179 PAAFTAHTGKDHWQVLLQARAIENTCYVIAPAQTGRHNSLRQSHGHAMIIDPWGVILADA 238
Query: 224 SETEEEVGVAEI 235
E V +AEI
Sbjct: 239 GEM-TGVAIAEI 249
>sp|P55177|YAG5_STAAU HYPOTHETICAL 29.8 KD PROTEIN IN AGR OPERON (ORF 5)
>gi|97824|pir||S20793 hypothetical protein 5 -
Staphylococcus aureus >gi|46506|emb|CAA36779.1| (X52543)
hypothetical protein [Staphylococcus aureus]
Length = 261
Score = 75.3 bits (182), Expect = 7e-13
Identities = 66/267 (24%), Positives = 119/267 (43%), Gaps = 15/267 (5%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
+KV Q+ + KN ++ + ++ +VVLPE+++ GY+ E E +
Sbjct: 1 MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNNGYDLEHLNEKADNNL 60
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTAEK-DGDVLYNSAVVVGPRG-FIGKYRKIHL--F 117
G++ +F+ +A V IVAG+ + ++N+A V G I +Y K+HL
Sbjct: 61 ----GQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSGQLINEYDKVHLVPM 116
Query: 118 YREKFFFEPGDLGFRVFDLGF-MKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPY 176
RE F G+ F L V +IC+D FPE R A GA + + A M
Sbjct: 117 LREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPARSGAKIAFYVAQWPMSR 176
Query: 177 APR---AMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASETEEEVGVA 233
+ RA+EN ++ + + G + ++ G S++ +P + + E E +
Sbjct: 177 LQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGD---LVGELNESADIL 233
Query: 234 EIDLYLVRNKRINDLNDIFKDRREEYY 260
+DL L ++ + +FK + + Y
Sbjct: 234 TVDLNLNEVEQQRENIPVFKSIKLDLY 260
>dbj|BAA77679.1| (AB027054) nitrilase-like protein [Oryza sativa]
Length = 362
Score = 75.3 bits (182), Expect = 7e-13
Identities = 75/278 (26%), Positives = 118/278 (41%), Gaps = 43/278 (15%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------- 48
V+ VQ + + KAE+LI+EA+ G+QLVV PE F GY
Sbjct: 31 VRATVVQASTVFYDTPATLDKAERLIEEAAGYGSQLVVFPEAFVGGYPRGSTFGFGANIS 90
Query: 49 -------NFETREEVFEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVV 101
E + A ++P G T L +A V++V G E++G LY S +
Sbjct: 91 IGNPKDKGKEEFRKYHAAAIEVP-GPEVTRLAAMAGKYKVFLVMGVIEREGYTLYCSVLF 149
Query: 102 VGPRG-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLA 159
P G ++GK+RK+ E+ + GD V+D K+G +IC++ P L
Sbjct: 150 FDPLGRYLGKHRKLMPTALERIIWGFGDGSTIPVYDTPLGKIGALICWENKMPLLRTALY 209
Query: 160 LKGADVIAHPANLVMPYAPRAMPIRALENKVYTVTADR-----------------VGEER 202
KG ++ P +M ALE + ++A++ +GEE
Sbjct: 210 GKGIEIYCAPTADSRQVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFTGLGEEP 269
Query: 203 GLKFI---GKSLIASPKAEVLSMASETEEEVGVAEIDL 237
+ G S+I SP EVL+ + E + A++DL
Sbjct: 270 SPDTVVCPGGSVIISPSGEVLAGPNYEGEALITADLDL 307
>pir||H83170 conserved hypothetical protein PA3797 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9949970|gb|AAG07184.1|AE004798_7 (AE004798)
conserved hypothetical protein [Pseudomonas aeruginosa]
Length = 264
Score = 74.5 bits (180), Expect = 1e-12
Identities = 63/205 (30%), Positives = 100/205 (48%), Gaps = 17/205 (8%)
Query: 31 SKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPE-GETTTFLMDVARDTGVYIVAGTAE 89
S GA LVVLPE+F TG++ + E Q PE G T +L++ AR G +
Sbjct: 37 SAAGADLVVLPEMFTTGFSMASAE------QAEPELGPTHAWLLEQARRLGAVVTGSLIV 90
Query: 90 KDGDVLY-NSAVVVGPRGFIGKYRKIHLFYR--EKFFFEPGDLGFRVFDLGFMKVGVMIC 146
+ D + N + P G + Y K HLF E + PG+ +F+L +V +IC
Sbjct: 91 QLADGSHRNRLLWARPDGEMLHYDKRHLFRMAGEHEHYSPGERQ-ELFELKGWRVRPLIC 149
Query: 147 FDWFFPESARTLALKGADVIAHPANLVMP---YAPRAMPIRALENKVYTVTADRVGEE-R 202
+D FP +R D++ + AN P + R +P RA+EN Y V +R+GE+
Sbjct: 150 YDLRFPVWSRD--PHDTDLLLYTANWPAPRRQHWNRLLPARAIENLCYVVAVNRIGEDGN 207
Query: 203 GLKFIGKSLIASPKAEVLSMASETE 227
L++ G S + + + L A++ +
Sbjct: 208 ALRYAGDSQVLDFQGDSLFNAADAD 232
>sp|P55175|Y601_SYNY3 HYPOTHETICAL 30.2 KD PROTEIN SLL0601 >gi|7449033|pir||S76524
hypothetical protein - Synechocystis sp. (strain PCC
6803) >gi|1001639|dbj|BAA10370.1| (D64002) hypothetical
protein [Synechocystis sp.]
Length = 272
Score = 73.0 bits (176), Expect = 3e-12
Identities = 75/242 (30%), Positives = 111/242 (44%), Gaps = 31/242 (12%)
Query: 18 KNYSKAEKLIKEASKQGAQLVVLPELFD-TGYNFETREEVFEIAQKIPEGETTTFLMDVA 76
+N +AE+LI A +QGA+LV LPE F G E E+ IA T FL +A
Sbjct: 19 ENLQEAEELIDLAVRQGAELVGLPENFAFLGNETEKLEQATAIATA-----TEKFLQTMA 73
Query: 77 RDTGVYIVAG----TAEKDGDVLYNSAVVVGPRGF-IGKYRKIHLF---------YREKF 122
+ V I+AG + YN+A ++ P G + +Y K+HLF Y E
Sbjct: 74 QRFQVTILAGGFPFPVAGEAGKAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWESA 133
Query: 123 FFEPGDLGFRVFDL-GFMKVGVMICFDWFFPESARTLALKGADVIAHPANLV----MPYA 177
G V+ F +G+ IC+D FPE R L+ +GADV+ PA +
Sbjct: 134 TVMAGQKYPPVYHSDSFGNLGLSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDHW 193
Query: 178 PRAMPIRALENKVYTVTADRVG---EERGLKFIGKSLIASPKAEVLSMASETEEEVGVAE 234
+ RA+EN Y + + G E R G ++I P +L+ A E + + +AE
Sbjct: 194 QVLLQARAIENTCYVIAPAQTGCHYERRHTH--GHAMIIDPWGVILADAGE-KPGLAIAE 250
Query: 235 ID 236
I+
Sbjct: 251 IN 252
>pir||T43198 nitrilase/Fhit protein - Caenorhabditis elegans
>gi|9955180|pdb|1EMS|A Chain A, Crystal Structure Of The
C. Elegans Nitfhit Protein >gi|9955181|pdb|1EMS|B Chain
B, Crystal Structure Of The C. Elegans Nitfhit Protein
>gi|3228664|gb|AAC39136.1| (AF069986) nitrilase and
fragile histidine triad fusion protein NitFhit
[Caenorhabditis elegans] >gi|6425404|emb|CAB60517.1|
(AL132860) contains similarity to Pfam domain: PF00795
(Carbon-nitrogen hydrolase), Score=419.1,
E-value=1.4e-122, N=1; PF01230 (HIT family), Score=97.2,
E-value=1.1e-25, N=1~cDNA EST yk103f2.3 comes from this
gene~cDNA EST yk103f2.5 comes from this ge>
Length = 440
Score = 72.6 bits (175), Expect = 5e-12
Identities = 67/264 (25%), Positives = 120/264 (45%), Gaps = 27/264 (10%)
Query: 17 DKNYSKAEKLIKEASKQGAQLVVLPELFD-TGYNFETREEVFEIAQKIPEGETTTFLMDV 75
+KN+ A+ +I+ A ++ ++V LPE FD G N + E ++A + E ++
Sbjct: 29 EKNFQAAKNMIERAGEKKCEMVFLPECFDFIGLN---KNEQIDLAM-ATDCEYMEKYREL 84
Query: 76 ARDTGVYIVAGTAE----KDGDVLYNSAVVVGPRGFI-GKYRKIHLF---------YREK 121
AR +++ G D +N+ +++ G +Y K+HLF E
Sbjct: 85 ARKHNIWLSLGGLHHKDPSDAAHPWNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLMES 144
Query: 122 FFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPA----NLVMPYA 177
F + G D ++G+ IC+D FPE + +GA +++ P+ N + +
Sbjct: 145 EFSKAGTEMIPPVDTPIGRLGLSICYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLAHW 204
Query: 178 PRAMPIRALENKVYTVTADRVGEERGLK-FIGKSLIASPKAEVLSMASETEEEVGVAEID 236
+ RA+EN+ Y V A + G + G S++ P V++ SE ++ AEID
Sbjct: 205 ETLLRARAIENQCYVVAAAQTGAHNPKRQSYGHSMVVDPWGAVVAQCSE-RVDMCFAEID 263
Query: 237 LYLVRNKRINDLNDIFKDRREEYY 260
L V R ++ +F RR + Y
Sbjct: 264 LSYVDTLR--EMQPVFSHRRSDLY 285
>dbj|BAB04808.1| (AP001510) unknown conserved protein [Bacillus halodurans]
Length = 512
Score = 72.6 bits (175), Expect = 5e-12
Identities = 67/202 (33%), Positives = 102/202 (50%), Gaps = 28/202 (13%)
Query: 81 VYIVAGT--AEKDGDVLYNSAVVVGPRGFIGKYRKIHLFYRE-KFFFEPGDLGFRVFDLG 137
+ I+ G+ E+DG + YN A + G I K K+H+ E KF+ G RVFD
Sbjct: 311 INIIGGSHFVEEDGKI-YNIAYLFRRDGTIEKQYKLHITPNERKFWGITGGDEVRVFDTD 369
Query: 138 FMKVGVMICFDWFFPESARTLALKGADVIAHP-------ANLVMPYAPRAMPIRALENKV 190
K+ + IC+D FPE AR KGA++I P L + Y +A RA+EN+V
Sbjct: 370 CGKIAIQICYDIEFPELARIAVDKGANIIFTPFCTDDRQGYLRVRYCSQA---RAIENQV 426
Query: 191 YTVTADRVG-----EERGLKFIGKSLIASP------KAEVLSMASETEEEVGVAEIDLYL 239
YTV + VG E +++ +S I SP + V+ S E V V ++DL +
Sbjct: 427 YTVLSGTVGNLPDVENMDIQY-AQSGIFSPSDFTFARDGVVGECSPNIETVVVGDVDLEI 485
Query: 240 V-RNKRINDLNDIFKDRREEYY 260
+ R++R +N + +DRR + Y
Sbjct: 486 LRRHRRAGSVNQL-RDRRHDIY 506
>gb|AAF69000.1|AF257487_1 (AF257487) amidase [Bacillus sp. BR449]
Length = 348
Score = 71.4 bits (172), Expect = 1e-11
Identities = 63/230 (27%), Positives = 104/230 (44%), Gaps = 12/230 (5%)
Query: 34 GAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDVAR-DT-GVYIVAGTAEKD 91
G LVV PE G ++ ++E+F A IP ET F + DT GV+ + G +D
Sbjct: 51 GMDLVVFPEYSTMGIMYD-QDEMFATAASIPGEETAIFAEACKKADTWGVFSLTGEKHED 109
Query: 92 --GDVLYNSAVVVGPRG-FIGKYRKIHLFYREKFFFEPGDLGFRVFDLGFMKVGVMICFD 148
YN+ V++ +G + KYRKI + + ++ PGD + +K+ +++C D
Sbjct: 110 HPNKAPYNTLVLINNKGEIVQKYRKIIPWCPIEGWY-PGDTTYVTEGPKGLKISLIVCDD 168
Query: 149 WFFPESARTLALKGADVIAHPANLVMPYAPRAMPI---RALENKVYTVTADRVGEERGLK 205
+PE R A+KGA++I + P + + + A N Y A+ G +
Sbjct: 169 GNYPEIWRDCAMKGAELIVRCQGYMYPAKEQQIMMAKAMAWANNTYVAVANATGFDGVYS 228
Query: 206 FIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRNKRIN--DLNDIFK 253
+ G S I L E + AE+ + +R+ R N N +FK
Sbjct: 229 YFGHSAIIGFDGRTLGECGTEENGIQYAEVSISQIRDFRKNAQSQNHLFK 278
>gb|AAK42348.1| Amidohydrolase, putative [Sulfolobus solfataricus]
Length = 270
Score = 71.0 bits (171), Expect = 1e-11
Identities = 72/254 (28%), Positives = 112/254 (43%), Gaps = 34/254 (13%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY-----NFETREEV 56
+KV Q+ + +NY K ++I+ +S A V+ PEL TGY +E ++
Sbjct: 3 IKVELAQIRSYPGDVYRNYKKHLEIIESSS---ADCVIFPELSLTGYIIKDLTYEIYKDA 59
Query: 57 FEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVV--GPRGFIGK-YRK 113
E QKI E + R + G +L NSA V+ G +I K Y
Sbjct: 60 EEATQKIAEKVNKCVVFGTIRQV----------RKG-ILRNSAAVIINGKLDYIYKFYLP 108
Query: 114 IHLFYREKFFFEPGD--LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPA- 170
+ + E+ +F+ GD ++F+ +K GV+IC D + PE L+L GAD I P+
Sbjct: 109 TYGLFEERRYFQRGDPLKDLKIFEYKDLKFGVVICEDAWHPEPIEALSLMGADAIFIPSA 168
Query: 171 --------NLVMPYA-PRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLS 221
NL + + + +L N V+TV + VG + F G S + SP +V
Sbjct: 169 SPMRKLRENLAIEESWDSLLKAHSLMNTVWTVFTNVVGSQEEEYFWGGSRVVSPLGDVKL 228
Query: 222 MASETEEEVGVAEI 235
E+ G EI
Sbjct: 229 KLKLFYEDRGTIEI 242
>sp|Q01360|ALAM_RHOER ALIPHATIC AMIDASE (WIDE SPECTRUM AMIDASE) >gi|98711|pir||JC1174
amidase (EC 3.5.1.4), aliphatic - Brevibacterium sp.
(strain R312) >gi|144094|gb|AAA22990.1| (M76451) amidase
[Brevibacterium sp.] >gi|152056|gb|AAA26186.1| (M88614)
aliphatic amidase [Rhodococcus erythropolis]
Length = 345
Score = 71.0 bits (171), Expect = 1e-11
Identities = 63/230 (27%), Positives = 103/230 (44%), Gaps = 12/230 (5%)
Query: 34 GAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMDVAR-DT-GVYIVAGTAEKD 91
G LVV PE G + EE++ A IP ET F DT G++ + G +D
Sbjct: 51 GMDLVVFPEYSTQGIMYN-EEEMYATAATIPGDETAIFSAACREADTWGIFSITGEQHED 109
Query: 92 --GDVLYNSAVVVGPRG-FIGKYRKIHLFYREKFFFEPGDLGFRVFDLGFMKVGVMICFD 148
YN+ +++ +G + +YRKI + + ++ PGD + +K+ ++IC D
Sbjct: 110 HPNKPPYNTLILIDNKGEIVQRYRKILPWCPIEGWY-PGDTTYVTEGPKGLKISLIICDD 168
Query: 149 WFFPESARTLALKGADVIAHPANLVMPYAPRAMPI---RALENKVYTVTADRVGEERGLK 205
+PE R A+KGA++I + P + + + A N Y A+ G +
Sbjct: 169 GNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVMMSKAMAWANNCYVAVANAAGFDGVYS 228
Query: 206 FIGKSLIASPKAEVLSMASETEEEVGVAEIDLYLVRNKRIND--LNDIFK 253
+ G S I L E E + A++ + +R+ R ND N IFK
Sbjct: 229 YFGHSAIIGFDGRTLGETGEEEYGIQYAQLSVSAIRDARENDQSQNHIFK 278
>sp|Q03217|NRL2_RHORH ALIPHATIC NITRILASE >gi|322251|pir||A45070 nitrilase - Rhodococcus
rhodochrous >gi|216934|dbj|BAA01994.1| (D11425)
nitrilase [Rhodococcus rhodochrous]
>gi|1665735|dbj|BAA11037.1| (D67026) nitrilase
[Rhodococcus rhodochrous]
Length = 366
Score = 71.0 bits (171), Expect = 1e-11
Identities = 69/276 (25%), Positives = 119/276 (43%), Gaps = 43/276 (15%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREE-----VF 57
KVA VQ P + K K +I EA++ G +LV PE+F GY + + +
Sbjct: 9 KVAAVQAQPVWFDAAKTVDKTVSIIAEAARNGCELVAFPEVFIPGYPYHIWVDSPLAGMA 68
Query: 58 EIAQKIPEGETT------TFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRG-FIGK 110
+ A + E T L+D ARD + +V G +E+DG LY + +V+ G + +
Sbjct: 69 KFAVRYHENSLTMDSPHVQRLLDAARDHNIAVVVGISERDGGSLYMTQLVIDADGQLVAR 128
Query: 111 YRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHP 169
RK+ + E+ + G+ V+D+ F ++G + C++ F ++ A+ H
Sbjct: 129 RRKLKPTHVERSVYGEGNGSDISVYDMPFARLGALNCWEHF--QTLTKYAMYSMHEQVHV 186
Query: 170 A-----NLVMPYAP--------RAMPIRALENKVYTVTADRV----------GEERGLKF 206
A +L P P A + ALE + + V +V + K
Sbjct: 187 ASWPGMSLYQPEVPAFGVDAQLTATRMYALEGQTFVVCTTQVVTPEAHEFFCDNDEQRKL 246
Query: 207 IGKS-----LIASPKAEVLSMASETEEEVGVAEIDL 237
IG+ +I ++ + +E EE + A+IDL
Sbjct: 247 IGRGGGFARIIGPDGRDLATPLAEDEEGILYADIDL 282
>sp|Q02068|NRL1_RHORH ALIPHATIC NITRILASE >gi|322250|pir||A43470 aliphatic nitrilase -
Rhodococcus rhodochrous >gi|216932|dbj|BAA02127.1|
(D12583) aliphatic nitrilase [Rhodococcus rhodochrous]
Length = 383
Score = 70.6 bits (170), Expect = 2e-11
Identities = 53/166 (31%), Positives = 78/166 (46%), Gaps = 13/166 (7%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNF-----ETREEV 56
VKVA VQ P IL+ D KA I+EA+K GA+ + PE++ GY + + + V
Sbjct: 13 VKVATVQAEPVILDADATIDKAIGFIEEAAKNGAEFLAFPEVWIPGYPYWAWIGDVKWAV 72
Query: 57 FEIAQKIPEGETT------TFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRG-FIG 109
+ K E T L AR + +V G +EKDG Y S V + G +
Sbjct: 73 SDFIPKYHENSLTLGDDRMRRLQLAARQNNIALVMGYSEKDGASRYLSQVFIDQNGDIVA 132
Query: 110 KYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPES 154
RK+ + E+ + G+ F D GF +VG + C++ F P S
Sbjct: 133 NRRKLKPTHVERTIYGEGNGTDFLTHDFGFGRVGGLNCWEHFQPLS 178
>dbj|BAA90460.1| (AB028892) nitrilase [Bacillus sp. OxB-1]
Length = 339
Score = 70.6 bits (170), Expect = 2e-11
Identities = 68/272 (25%), Positives = 115/272 (42%), Gaps = 39/272 (14%)
Query: 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTG-----------YNFE 51
+VA VQ +P +L+ D K +L+ EA+ GA+++ PE F G Y +
Sbjct: 8 RVAAVQASPVLLDLDATIDKTCRLVDEAAANGAKVIAFPEAFIPGYPWWIWLGNADYGMK 67
Query: 52 TREEVFEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRG-FIGK 110
++++ + +IP + A VY EKDG LY + + P G IGK
Sbjct: 68 YYIQLYKNSVEIP--SLAVQKLSSAGTNKVYFCVSVTEKDGGSLYLTQLWFDPNGDLIGK 125
Query: 111 YRKIHLFYREKFFFEPGDLGFR-VFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHP 169
+RK+ EK + GD VF+ F +G + C++ F P + +A V
Sbjct: 126 HRKLKATNAEKTIWGDGDGSMMPVFETEFGNLGGLQCWEHFLPLNVAAMASMNEQVHVAS 185
Query: 170 ANLVMP-----YAPR----AMPIRALENKVYTVTADRV------------GEERGLKFIG 208
+ MP + P A A+ N+V+ + + ++ E+R +G
Sbjct: 186 WPIGMPQEGHLFGPEQCVTATKYYAISNQVFCLLSSQIWTEEQRDKICETEEQRNFMKVG 245
Query: 209 ---KSLIASPKAEVLSMASETEEEVGVAEIDL 237
+IA E+ + + EE + A+IDL
Sbjct: 246 HGFSKIIAPNGMEIGNKLAHDEEGITYADIDL 277
>sp|P46011|NRL4_ARATH NITRILASE 4 >gi|508737|gb|AAA19628.1| (U09961) nitrilase
[Arabidopsis thaliana] >gi|9757810|dbj|BAB08328.1|
(AB007651) Nitrilase 4 [Arabidopsis thaliana]
Length = 355
Score = 70.2 bits (169), Expect = 2e-11
Identities = 73/278 (26%), Positives = 115/278 (41%), Gaps = 45/278 (16%)
Query: 1 MVKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEI- 59
+V+ VQ + + KAE+L+ EA++ G+QLVV PE F GY R FE+
Sbjct: 35 IVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQLVVFPEAFIGGY---PRGSTFELA 91
Query: 60 ------------------AQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVV 101
A +P E + +A+ VY+V G E++G LY + +
Sbjct: 92 IGSRTAKGRDDFRKYHASAIDVPGPEVERLAL-MAKKYKVYLVMGVIEREGYTLYCTVLF 150
Query: 102 VGPRG-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLA 159
+G F+GK+RK+ E+ + GD VFD K+G IC++ P +
Sbjct: 151 FDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIGKIGAAICWENRMPSLRTAMY 210
Query: 160 LKGADVIAHPANLVMPYAPRAMPIRALENKVYTVTADRV---------------GEERGL 204
KG ++ P +M ALE + ++A++ G E L
Sbjct: 211 AKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFCRRKDYPSPPEYMFSGSEESL 270
Query: 205 K-----FIGKSLIASPKAEVLSMASETEEEVGVAEIDL 237
G S I SP VL+ + E + A++DL
Sbjct: 271 TPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDL 308
>gi|6322522 52-kDa amidase specific for N-terminal asparagine and glutamine;
Nta1p [Saccharomyces cerevisiae]
>gi|730197|sp|P40354|NTA1_YEAST PROTEIN N-TERMINAL
AMIDASE (NT-AMIDASE) >gi|626099|pir||S47938
amino-terminal amidase (EC 3.5.1.-) NTA1 - yeast
(Saccharomyces cerevisiae) >gi|531232|gb|AAB59320.1|
(L35564) N-terminal amidase [Saccharomyces cerevisiae]
>gi|1015735|emb|CAA89590.1| (Z49562) ORF YJR062c
[Saccharomyces cerevisiae] >gi|1019684|gb|AAB39288.1|
(L47993) ORF YJR062c [Saccharomyces cerevisiae]
Length = 457
Score = 70.2 bits (169), Expect = 2e-11
Identities = 47/146 (32%), Positives = 74/146 (50%), Gaps = 7/146 (4%)
Query: 2 VKVAYVQMNPQILEPD----KNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVF 57
+KV +Q+NPQI + D + +S +K+ K A+ +++ PE TGY+F R+++
Sbjct: 19 LKVLVIQLNPQIGQVDQTIKRTWSILDKVTKSATYVKPDIILFPEFALTGYSFHARKDIL 78
Query: 58 EIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGD-VLYNSAVVVGPRG-FIGKYRKIH 115
K EG + ++ Y + G E D + LYNSA+VV P+G I YRK
Sbjct: 79 PYVTKKDEGPSFELAKSISEKFQCYTIIGYPEDDDEQKLYNSALVVNPQGEQIFNYRKTF 138
Query: 116 LFYRE-KFFFEPGDLGFRVFDLGFMK 140
L+ E + E GF+ F + F K
Sbjct: 139 LYDTEMNWDCEENPEGFQTFPMDFSK 164
>pir||B81199 nitrilase NMB0441 [imported] - Neisseria meningitidis (group B
strain MD58) >gi|7225666|gb|AAF40879.1| (AE002400)
nitrilase [Neisseria meningitidis MC58]
Length = 270
Score = 69.9 bits (168), Expect = 3e-11
Identities = 78/262 (29%), Positives = 119/262 (44%), Gaps = 27/262 (10%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQ 61
++VA VQM + P+ N + ++L+ A++QGA V+LPE + +T + +A+
Sbjct: 4 IRVAAVQMVSGV-SPETNVAAMKRLVARAAEQGADWVLLPEYWVLMGANDT--DKLALAE 60
Query: 62 KIPEGETTTFLMDVARDTGVYIVAGTA---EKDGDVLYNSAVVVGPRGF-IGKYRKIHLF 117
+ G T L + A++ GV + GT + + N+ +V G G G Y K+HLF
Sbjct: 61 PLGGGRFQTALSETAKECGVVLFGGTVPLQSCEAGKVMNTLLVYGRDGVRTGLYHKMHLF 120
Query: 118 --------YREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHP 169
Y E G + G + V IC+D FPE R DV+ P
Sbjct: 121 GFSGLGERYAEADTIRAGGDVPHLSAEG-VPVAAGICYDVRFPEFFRRQL--PFDVLMLP 177
Query: 170 ANLV----MPYAPRAMPIRALENKVYTVTADRVG-EERGLKFIGKSLIASPKAEVLSMAS 224
A + + RA+EN+ Y V A + G E G + G S+I P +VL +
Sbjct: 178 AAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRRTFGHSMIVDPWGDVLDVLP 237
Query: 225 ETEEEVGVAEID---LYLVRNK 243
E E V A+ID L VRN+
Sbjct: 238 EGEGVV-TADIDANRLNSVRNR 258
>sp|Q42965|NRL4_TOBAC NITRILASE 4 >gi|7435979|pir||T03736 nitrilase (EC 3.5.5.1) - common
tobacco >gi|1171482|dbj|BAA09645.1| (D63331) nitrilase
[Nicotiana tabacum]
Length = 349
Score = 69.5 bits (167), Expect = 4e-11
Identities = 74/274 (27%), Positives = 111/274 (40%), Gaps = 39/274 (14%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------- 48
V+ VQ + + KAE+L+ EA+ GAQLVV PE F GY
Sbjct: 29 VRATVVQASTIFYDTPATLVKAERLLAEAASYGAQLVVFPEAFIGGYPRGSTFGVSIGNR 88
Query: 49 ---NFETREEVFEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPR 105
E + A +P G L +A VY+V G E+DG LY + + +
Sbjct: 89 TAKGKEEFRKYHASAIDVP-GPEVDRLAAMAGKYKVYLVMGVIERDGYTLYCTVLFFDSQ 147
Query: 106 G-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
G F+GK+RKI E+ + GD V+D K+G IC++ P + KG
Sbjct: 148 GHFLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIGAAICWENRMPLLRTAMYAKGI 207
Query: 164 DVIAHPANLVMPYAPRAMPIRALENKVYTVTADRV---------------GEERGLK--- 205
++ P +M ALE + ++A++ G E L
Sbjct: 208 EIYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEDLTPDS 267
Query: 206 --FIGKSLIASPKAEVLSMASETEEEVGVAEIDL 237
G S+I SP VL+ + E + A++DL
Sbjct: 268 IVCAGGSVIISPSGAVLAGPNYVGEALISADLDL 301
>pir||A72454 probable nitrilase APE2277 - Aeropyrum pernix (strain K1)
>gi|5105978|dbj|BAA81289.1| (AP000064) 287aa long
hypothetical nitrilase [Aeropyrum pernix]
Length = 287
Score = 69.5 bits (167), Expect = 4e-11
Identities = 69/238 (28%), Positives = 100/238 (41%), Gaps = 22/238 (9%)
Query: 15 EPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPEGETTTFLMD 74
E D N ++L K +V+ PE R+ +++ A+ + EG + L
Sbjct: 32 EKDANLESVKRLASRV-KNSPDIVLTPEYLMLDPTGLGRDAIYDAAEDL-EGRWSRELSK 89
Query: 75 VARDTGVYIVAGTAEKDGDVLYNSAVVVGPR--GFIGKYRKIHLF----YREKFFFEPGD 128
+A G ++ K +A V+ R G IG YRK HLF Y E F EPGD
Sbjct: 90 IAESLGSCLLGHLFLKTPSGRVANAAVLYSRDGGIIGVYRKTHLFDAYGYVESSFTEPGD 149
Query: 129 LGFRVFDLGFMKVGVMICFDWFFPESARTLAL-KGADVIAHPANLVM-PYAPRAMPI--- 183
+ +GV IC++ FPE RT +L G D+ PA P A+ +
Sbjct: 150 ELWEPRKACGASIGVAICYELRFPEIFRTQSLVGGVDIFLVPAAWYRGPGKEEALSVLSR 209
Query: 184 -RALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASE----TEEEVGVAEID 236
RA EN Y A G F+G+S+I P L+ A E E+ + EI+
Sbjct: 210 ARAQENTSYVAVASNA----GANFVGRSMIIHPLGYTLAQAPPWEWVLEHEIDLREIE 263
>gi|9055236 VNN3 protein [Homo sapiens] >gi|7160973|emb|CAB76824.1| (AJ238982)
VNN3 protein [Homo sapiens]
Length = 501
Score = 69.5 bits (167), Expect = 4e-11
Identities = 56/178 (31%), Positives = 79/178 (43%), Gaps = 30/178 (16%)
Query: 7 VQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPE- 65
V +L +KN EK +K A+KQGA ++V PE G+ F TRE ++ + IP+
Sbjct: 45 VSKEEALLLMNKNIDVLEKAVKLAAKQGAHIIVTPEDGIYGWIF-TRESIYPYLEDIPDP 103
Query: 66 -------------GETTTF--LMDVARDTGVYIVAGTAEK------------DGDVLYNS 98
G T L +A+D +Y+VA +K DG YN+
Sbjct: 104 GVNWIPCRDPWRFGNTPVQQRLSCLAKDNSIYVVANIGDKKPCNASDSQCPPDGRYQYNT 163
Query: 99 AVVVGPRG-FIGKYRKIHLFYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESA 155
VV +G + +Y K +LF E F P D FD F K G+ CFD F + A
Sbjct: 164 DVVFDSQGKLLARYHKYNLFAPEIQFDFPKDSELVTFDTPFGKFGIFTCFDIFSHDPA 221
>gi|11418681 VNN3 protein [Homo sapiens]
Length = 274
Score = 69.5 bits (167), Expect = 4e-11
Identities = 56/178 (31%), Positives = 79/178 (43%), Gaps = 30/178 (16%)
Query: 7 VQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIAQKIPE- 65
V +L +KN EK +K A+KQGA ++V PE G+ F TRE ++ + IP+
Sbjct: 45 VSKEEALLLMNKNIDVLEKAVKLAAKQGAHIIVTPEDGIYGWIF-TRESIYPYLEDIPDP 103
Query: 66 -------------GETTTF--LMDVARDTGVYIVAGTAEK------------DGDVLYNS 98
G T L +A+D +Y+VA +K DG YN+
Sbjct: 104 GVNWIPCRDPWRFGNTPVQQRLSCLAKDNSIYVVANIGDKKPCNASDSQCPPDGRYQYNT 163
Query: 99 AVVVGPRG-FIGKYRKIHLFYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESA 155
VV +G + +Y K +LF E F P D FD F K G+ CFD F + A
Sbjct: 164 DVVFDSQGKLLARYHKYNLFAPEIQFDFPKDSELVTFDTPFGKFGIFTCFDIFSHDPA 221
>pir||T03739 nitrilase (EC 3.5.5.1) 4B - common tobacco
>gi|1181615|dbj|BAA11770.1| (D83078) nitrilase
[Nicotiana tabacum]
Length = 348
Score = 68.3 bits (164), Expect = 9e-11
Identities = 70/273 (25%), Positives = 110/273 (39%), Gaps = 38/273 (13%)
Query: 2 VKVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGY------------- 48
V+ VQ + + KAE+L+ EA+ GAQLVV PE F GY
Sbjct: 29 VRATVVQASTIFYDTPATLDKAERLLAEAASYGAQLVVFPEAFIGGYPRGSTFGVSIGNR 88
Query: 49 ---NFETREEVFEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPR 105
E + A +P G L +A VY+V G E+DG LY + + +
Sbjct: 89 TAKGKEEFRKYHASAIDVP-GPEVDRLAAMAGKYKVYLVMGVIERDGYTLYCTVLFFDSQ 147
Query: 106 G-FIGKYRKIHLFYREKFFFEPGD-LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGA 163
G ++GK+RKI E+ + GD V+D K+G IC++ P + KG
Sbjct: 148 GHYLGKHRKIMPTALERIIWGFGDGSTIPVYDTPLGKIGAAICWENRMPLLRTAMYAKGI 207
Query: 164 DVIAHPANLVMPYAPRAMPIRALENKVYTVTADRVGEER-------------------GL 204
++ P +M ALE + ++A++ + +
Sbjct: 208 EIYCAPTADSRDVWQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFSGTEDLTPDSI 267
Query: 205 KFIGKSLIASPKAEVLSMASETEEEVGVAEIDL 237
G S+I SP VL+ + E + A++DL
Sbjct: 268 VCAGGSVIISPSGAVLAGPNYEGEALISADLDL 300
>pir||G75274 nitrilase-related protein - Deinococcus radiodurans (strain R1)
>gi|6460248|gb|AAF11974.1|AE002073_4 (AE002073)
nitrilase-related protein [Deinococcus radiodurans]
Length = 293
Score = 68.3 bits (164), Expect = 9e-11
Identities = 59/184 (32%), Positives = 91/184 (49%), Gaps = 18/184 (9%)
Query: 27 IKEASKQGAQLVVLPE--------LFDTGYNFETREEVFEIAQKIPEGETTTFLMDVARD 78
++EA++Q AQL+V PE L G + + R + + +PE +AR+
Sbjct: 34 VEEAARQRAQLLVFPEYAPLELISLLPAGLHHDLRGLLPALQPLLPE--VLALHERLARE 91
Query: 79 TGVYIVAGTAE-KDGDVLYNSAVVVGPRGFIGKYRKIHL--FYREKFFFEPGDLGFRVFD 135
GV +VAG+ G N A V GP+G +G K+ + F E++ +PG G RVF
Sbjct: 92 HGVTLVAGSFPVAHGAGYVNRAHVFGPQGPLGHQDKLLMTRFEAEEWGIDPG-AGVRVFG 150
Query: 136 LGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPI----RALENKVY 191
G ++ GV IC+D FP AR A GA+++ P+ + + RALEN+ Y
Sbjct: 151 AGGVQFGVAICYDSEFPHLARAQAELGAELLVVPSFTASRAGFTRVRVGSMARALENQCY 210
Query: 192 TVTA 195
+ A
Sbjct: 211 ALHA 214
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.140 0.402
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91914476
Number of Sequences: 2977
Number of extensions: 3746670
Number of successful extensions: 9349
Number of sequences better than 1.0e-10: 90
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 86
Number of HSP's that attempted gapping in prelim test: 9177
Number of HSP's gapped (non-prelim): 92
length of query: 262
length of database: 189,106,746
effective HSP length: 55
effective length of query: 207
effective length of database: 156,189,961
effective search space: 32331321927
effective search space used: 32331321927
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 164 (68.3 bits)