BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1490 (thrS) DE:threonyl-tRNA synthetase (thrS) (625 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||C75044 threonyl-tRNA synthetase (thrs) PAB1490 - Pyrococcus... 1262 0.0 sp|O58430|SYT_PYRHO THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 1186 0.0 gi|11498158 threonyl-tRNA synthetase (thrS) [Archaeoglobus fulgi... 775 0.0 sp|Q58597|SYT_METJA THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 581 e-165 pir||D64449 threonine--tRNA ligase (EC 6.1.1.3) - Methanococcus ... 581 e-165 sp|O27504|SYT_METTH THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 574 e-162 sp|P56881|SYT_THETH THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 184 3e-45 sp|P18255|SYT1_BACSU THREONYL-TRNA SYNTHETASE 1 (THREONINE--TRNA... 182 8e-45 sp|P18256|SYT2_BACSU THREONYL-TRNA SYNTHETASE 2 (THREONINE--TRNA... 177 4e-43 emb|CAB02510.1| (Z80360) Threonyl tRNA Synthetase [Bacillus subt... 177 4e-43 sp|Q55806|SYT_SYNY3 THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 176 6e-43 gb|AAG20043.1| (AE005084) threonyl-tRNA synthetase; ThrS [Haloba... 174 3e-42 pir||B75317 threonyl-tRNA synthetase - Deinococcus radiodurans (... 173 5e-42 dbj|BAB06860.1| (AP001517) threonyl-tRNA synthetase 1 [Bacillus ... 171 3e-41 gb|AAK42622.1| Threonyl-tRNA synthetase (thrS) [Sulfolobus solfa... 165 2e-39 sp|Q9WZJ9|SYT_THEMA THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 163 6e-39 sp|O05947|SYT_RICPR THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 162 8e-39 sp|O67583|SYT_AQUAE THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 161 2e-38 pir||A82479 threonyl-tRNA synthetase VCA0287 [imported] - Vibrio... 160 5e-38 pir||H82878 threonyl-tRNA synthetase UU534 [imported] - Ureaplas... 157 4e-37 sp|O51662|SYT_BORBU THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 155 1e-36 sp|Q9ZMV3|SYT_HELPJ THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 153 4e-36 sp|P56071|SYT_HELPY THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 150 3e-35 sp|O83809|SYT_TREPA THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 150 6e-35 pir||T51624 threonine--tRNA ligase (EC 6.1.1.3) [imported] - Ara... 149 1e-34 pir||B81939 probable threonine--tRNA ligase (EC 6.1.1.3) NMA0929... 148 1e-34 pir||E81167 threonyl-tRNA synthetase NMB0720 [imported] - Neisse... 148 2e-34 gb|AAF53166.1| (AE003635) CG5353 gene product [alt 1] [Drosophil... 147 3e-34 sp|O07151|SYT_MYCLE THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 147 3e-34 dbj|BAA15486.1| (D90813) Threonine--tRNA ligase (EC 6.1.1.3) [Es... 146 5e-34 pdb|1EVK|A Chain A, Crystal Structure Of A Truncated Form Of Thr... 146 5e-34 sp|P00955|SYT_ECOLI THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 146 5e-34 emb|CAA23560.1| (V00291) threonyl-tRNA synthetase (thrS; EC 6.1.... 146 5e-34 emb|CAB70933.1| (AL137778) threonine-tRNA synthetase [Streptomyc... 143 7e-33 sp|P52709|SYTC_CAEEL PROBABLE THREONYL-TRNA SYNTHETASE, CYTOPLAS... 142 1e-32 sp|O06200|SYT_MYCTU THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 139 6e-32 sp|P57225|SYT_BUCAI THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 139 6e-32 sp|O04630|SYTC_ARATH PROBABLE THREONYL-TRNA SYNTHETASE, CYTOPLAS... 139 1e-31 sp|Q9Z7A0|SYT_CHLPN THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 138 2e-31 sp|O84585|SYT_CHLTR THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 138 2e-31 pir||G71497 threonine--tRNA ligase (EC 6.1.1.3) - Chlamydia trac... 138 2e-31 sp|P47615|SYT_MYCGE THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 137 3e-31 sp|P75225|SYT_MYCPN THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 137 3e-31 pir||F83303 threonyl-tRNA synthetase PA2744 [imported] - Pseudom... 136 7e-31 pir||D82770 threonyl-tRNA synthetase XF0736 [imported] - Xylella... 135 2e-30 pir||A81656 threonyl-tRNA synthetase TC0870 [imported] - Chlamyd... 134 3e-30 sp|P43014|SYT_HAEIN THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 133 5e-30 gi|4507367 threonyl-tRNA synthetase [Homo sapiens] >gi|135177|sp... 133 6e-30 sp|P87144|SYTC_SCHPO THREONYL-TRNA SYNTHETASE, CYTOPLASMIC (THRE... 129 9e-29 emb|CAC11475.1| (AL445064) threonine--tRNA ligase related protei... 129 1e-28 emb|CAC10316.1| (AL442629) SCH63.25, possible threonyl tRNA synt... 128 2e-28 pir||E81439 threonine--tRNA ligase (EC 6.1.1.3) Cj0206 [imported... 126 8e-28 gb|AAK40712.1| Threonyl-tRNA synthetase homolog (thrS-like) [Sul... 118 1e-25 sp|P46244|SYT_BUCAP THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 116 7e-25 emb|CAA21975.1| (AL033497) mitochondrial threonyl tRNA synthetas... 116 7e-25 gb|AAF97275.1|AC034106_18 (AC034106) Strong similarity to threon... 116 9e-25 gi|6322654 mitochondrial threonine-tRNA synthetase; Mst1p [Sacch... 113 8e-24 gb|AAA34808.1| (M12087) thr-tRNA-synthetase [Saccharomyces cerev... 112 1e-23 emb|CAA26666.1| (X02906) threonyl-tRNA synthetase (aa 1-734) [Sa... 111 3e-23 gi|6322112 Threonyl-tRNA synthetase, cytoplasmic; Ths1p [Sacchar... 111 3e-23 dbj|BAB14117.1| (AK022590) unnamed protein product [Homo sapiens] 102 9e-21 sp|O13969|SYTM_SCHPO PROBABLE THREONYL-TRNA SYNTHETASE, MITOCHON... 99 2e-19 sp|Q9YFY3|SYT2_AERPE PROBABLE THREONYL-TRNA SYNTHETASE 2 (THREON... 85 2e-15 sp|P52833|SYT_PSESY THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LI... 85 3e-15 gb|AAD34790.1|AF136400_4 (AF136400) threonyl-tRNA synthetase [Ps... 80 1e-13 gb|AAF78372.1|AC069551_5 (AC069551) T10O22.10 [Arabidopsis thali... 80 1e-13 gb|AAF97829.1|AC034107_12 (AC034107) Contains similarity to thre... 73 9e-12 >pir||C75044 threonyl-tRNA synthetase (thrs) PAB1490 - Pyrococcus abyssi (strain Orsay) >gi|5458761|emb|CAB50248.1| (AJ248287) threonyl-tRNA synthetase (thrS) [Pyrococcus abyssi] Length = 625 Score = 1262 bits (3231), Expect = 0.0 Identities = 625/625 (100%), Positives = 625/625 (100%) Query: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA Sbjct: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 Query: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY Sbjct: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 Query: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFD 180 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFD Sbjct: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFD 180 Query: 181 FTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL 240 FTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL Sbjct: 181 FTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL 240 Query: 241 LEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG 300 LEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG Sbjct: 241 LEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG 300 Query: 301 QFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK 360 QFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK Sbjct: 301 QFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK 360 Query: 361 EEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQ 420 EEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQ Sbjct: 361 EEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQ 420 Query: 421 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA 480 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA Sbjct: 421 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA 480 Query: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV Sbjct: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 Query: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK 600 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK Sbjct: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK 600 Query: 601 EKTEGFPYKPRPLPLLLSKRPKFRG 625 EKTEGFPYKPRPLPLLLSKRPKFRG Sbjct: 601 EKTEGFPYKPRPLPLLLSKRPKFRG 625 >sp|O58430|SYT_PYRHO THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7450857|pir||D71116 threonine--tRNA ligase (EC 6.1.1.3) - Pyrococcus horikoshii >gi|3257107|dbj|BAA29790.1| (AP000003) 625aa long hypothetical threonyl-tRNA synthetase [Pyrococcus horikoshii] Length = 625 Score = 1186 bits (3035), Expect = 0.0 Identities = 575/625 (92%), Positives = 607/625 (97%) Query: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 MR+LLIHSDYIEYEVKDKA+KNPEPISE+ K+GRM+EVLVAFISVEKVDE NPEEV KA Sbjct: 1 MRILLIHSDYIEYEVKDKAIKNPEPISEEEKKGRMDEVLVAFISVEKVDETNPEEVVSKA 60 Query: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 +EEI VA QVKAENVFVYPFAHLSSELAKPSVA +IL +VY+GLKE+G+NVGKAPFGYY Sbjct: 61 VEEIINVASQVKAENVFVYPFAHLSSELAKPSVAQEILRKVYEGLKEKGYNVGKAPFGYY 120 Query: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFD 180 KAFKISCKGHPLAELSRTI+PE A+ EEVPEAL+KEE ELVSYWYILTPEGELIEVDKFD Sbjct: 121 KAFKISCKGHPLAELSRTIIPEGAKEEEVPEALKKEETELVSYWYILTPEGELIEVDKFD 180 Query: 181 FTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL 240 FTGYENLRKF NYEI+K+RIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL Sbjct: 181 FTGYENLRKFANYEISKSRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL 240 Query: 241 LEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG 300 LEQYVTEKVIEYGAMEVETP+MYDFEHPALEKYLNRFPARQYIVLSGDK+YFLRFAACFG Sbjct: 241 LEQYVTEKVIEYGAMEVETPVMYDFEHPALEKYLNRFPARQYIVLSGDKKYFLRFAACFG 300 Query: 301 QFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK 360 QF+I KDA+ISYR+LPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKD+EQAK Sbjct: 301 QFLISKDAVISYRHLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDLEQAK 360 Query: 361 EEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQ 420 +EFKKQFKLSMEVL+GVGLTPEDYEVAIRFTEDFWKE++DFIVELVK+IGKPVLIEMWKQ Sbjct: 361 DEFKKQFKLSMEVLKGVGLTPEDYEVAIRFTEDFWKENRDFIVELVKIIGKPVLIEMWKQ 420 Query: 421 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA 480 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGI YYDENGEEKYPLILHCSPSGA Sbjct: 421 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIKYYDENGEEKYPLILHCSPSGA 480 Query: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 IERVMYAILEKQAKLM EGKKPM PLWLSPIQVRVIPVSEEYLDYALY+AGKLEGA+IRV Sbjct: 481 IERVMYAILEKQAKLMQEGKKPMLPLWLSPIQVRVIPVSEEYLDYALYIAGKLEGARIRV 540 Query: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK 600 DVDDEDERLNKKIRRAEKEWIPYIVVVGA+EKE+GTITVRRREDGKQYETR+EELIKEIK Sbjct: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAKEKESGTITVRRREDGKQYETRLEELIKEIK 600 Query: 601 EKTEGFPYKPRPLPLLLSKRPKFRG 625 EK EGFPYKPRPLPLLLSKRPKFRG Sbjct: 601 EKVEGFPYKPRPLPLLLSKRPKFRG 625 >gi|11498158 threonyl-tRNA synthetase (thrS) [Archaeoglobus fulgidus] >gi|3334363|sp|O29703|SYT_ARCFU THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7450858|pir||D69318 threonyl-tRNA synthetase (thrS) homolog - Archaeoglobus fulgidus >gi|2650076|gb|AAB90685.1| (AE001066) threonyl-tRNA synthetase (thrS) [Archaeoglobus fulgidus] Length = 619 Score = 775 bits (1980), Expect = 0.0 Identities = 388/629 (61%), Positives = 487/629 (76%), Gaps = 15/629 (2%) Query: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 M++LLIH+DY+EYEVK K K EP D K R+EEVLVAF SVEK D++N V KA Sbjct: 1 MKLLLIHADYMEYEVKKKT-KLAEPF--DGKGERVEEVLVAFTSVEKGDDEN---VVRKA 54 Query: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 E I +VAE+V AE + +YP+AHLSS LA A+ +L ++ L + F V ++PFG+Y Sbjct: 55 AEAIREVAEKVNAERIMIYPYAHLSSNLADAETAVKLLKQLEAELSD--FEVHRSPFGWY 112 Query: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFD 180 KAF+ISCKGHPL+ELSR I E EV +AL+ EEE++VSYWYILTPE EL+EV+KFD Sbjct: 113 KAFRISCKGHPLSELSREIGGEAEA--EVTQALKDEEEKVVSYWYILTPEKELVEVEKFD 170 Query: 181 FTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL 240 FTGYE LRKFVNYEIAK R + PPHV+ M EL DYEP SD G++RYYPKGRL+K+L Sbjct: 171 FTGYEKLRKFVNYEIAKRRAVDVTPPHVEYMRRLELADYEPASDSGHIRYYPKGRLVKTL 230 Query: 241 LEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG 300 LEQ++T K I+YGAMEVETPIMYD HP L +YL RFPARQYI+ + +FLRFAACFG Sbjct: 231 LEQFITRKCIDYGAMEVETPIMYDRNHPTLRRYLERFPARQYIIKGDKREFFLRFAACFG 290 Query: 301 QFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK 360 QF++ + I+YRNLPL++YELTRYSFR+E+RGEL GLRRLRAFTMPDMHT+AKD+EQAK Sbjct: 291 QFLMLSSSTITYRNLPLKIYELTRYSFRKEQRGELVGLRRLRAFTMPDMHTVAKDMEQAK 350 Query: 361 EEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQ 420 EEF Q++LS+EVL +GL PEDYEVA+R T+DF++E+++F+ LV ++ KP+LIEMW Sbjct: 351 EEFFNQYRLSVEVLREIGLEPEDYEVAVRITKDFYEENREFVHSLVDILKKPILIEMWDH 410 Query: 421 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA 480 RFFYF+LKFEFNFVD LDKA+ALSTVQIDVENAER+GIT+ D +G+EK+P ILHCS SGA Sbjct: 411 RFFYFVLKFEFNFVDALDKASALSTVQIDVENAERYGITFVDSDGKEKHPYILHCSVSGA 470 Query: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 +ERVMYA+LEK +++EG+ PM P+WLSP QVRVIPVSE ++D A+ +A + IRV Sbjct: 471 VERVMYALLEKAKFMLDEGRLPMLPVWLSPTQVRVIPVSERFVDAAIKIADDIARNGIRV 530 Query: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENG--TITVR---RREDGKQYETRIEEL 595 DVDD +E L KKIR A+ EWIPYI VVG +E E+G +TVR +++ K+ E EEL Sbjct: 531 DVDDRNETLGKKIRDAQTEWIPYIAVVGEKEIESGKLAVTVRAESTQKEQKRVEMSAEEL 590 Query: 596 IKEIKEKTEGFPYKPRPLPLLLSKRPKFR 624 + ++ + EG P+ P PLP LLS RP FR Sbjct: 591 AERVRSECEGKPFMPLPLPKLLSLRPSFR 619 >sp|Q58597|SYT_METJA THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) Length = 620 Score = 581 bits (1482), Expect = e-165 Identities = 309/636 (48%), Positives = 427/636 (66%), Gaps = 30/636 (4%) Query: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 M++LLIHSDY+E+E K+K E +E++K G+++E L FI+VE+ DE NPE ++ A Sbjct: 1 MKMLLIHSDYLEFEAKEKTKIAEE--TENLK-GKLDECLACFIAVEREDENNPEGTAIGA 57 Query: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 +EEI KVA Q+K N+ VYP+AHLSS+L+ P A+ +L + LKERG+NV +APFG+Y Sbjct: 58 VEEIEKVANQLKVNNIVVYPYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGWY 117 Query: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGE-LIEVDK- 178 KAFKISCKGHPL+ELSR IV +E + EE E S +Y+L PE E +IE+++ Sbjct: 118 KAFKISCKGHPLSELSRKIVAKEEKKEEGEE----------SKFYLLNPETEEIIELNEN 167 Query: 179 -FDFTGYENLRKFVNYEIAKNRIAEK-EPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRL 236 + E L +E+ E EPPHVK + E ++ YE SDPG+ R+YPKG+L Sbjct: 168 NINIIKDEELLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKL 227 Query: 237 IKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFA 296 ++ LL YV V+ GAM VETPIMYD +PA+ ++ ++F RQY G+K LRFA Sbjct: 228 MRDLLADYVYNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFA 287 Query: 297 ACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDI 356 ACFGQFM+KKD + R LPL++YEL+ YSFR E+RGEL GL+RLR FTMPDMHT+ ++ Sbjct: 288 ACFGQFMMKKDMYLLPRYLPLKLYELSTYSFRYEQRGELVGLKRLRCFTMPDMHTVCLNL 347 Query: 357 EQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVK----LIGKP 412 EQA EEF+KQF ++ + + L+ Y V RFT+DF+ EH+D+ ++ K GK Sbjct: 348 EQAMEEFEKQFWECLKTGDDLNLS---YSVIFRFTKDFFDEHRDWFFKIAKEYKNKYGKD 404 Query: 413 VLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLI 472 V++E+ +R Y++ K + +D+L + TVQIDVE+A+RF I + G E YP+I Sbjct: 405 VILEILPKRKHYWVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEG-EIYPII 463 Query: 473 LHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGK 532 LHCSP+G+IERV+ +LEK A +G PM P+WLSPIQVRVIPV+E + DYAL VA K Sbjct: 464 LHCSPTGSIERVLCGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEK 523 Query: 533 LEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDG---KQYE 589 L IR D DD +E ++KKIR A KEW+PY+VV+G E E+ +TV RE K Y+ Sbjct: 524 LRENNIRADFDDREESVSKKIRNAGKEWVPYVVVIGDEEMESDKLTVTIREKSTLKKPYK 583 Query: 590 TR--IEELIKEIKEKTEGFPYKPRPLPLLLSKRPKF 623 + ++ELI+ IK++T +PY+P PLP+ S +PKF Sbjct: 584 EKMTLDELIERIKKETANYPYRPLPLPIRCSLQPKF 619 >pir||D64449 threonine--tRNA ligase (EC 6.1.1.3) - Methanococcus jannaschii >gi|1591826|gb|AAB99201.1| (U67561) threonyl-tRNA synthetase (thrS) [Methanococcus jannaschii] Length = 624 Score = 581 bits (1482), Expect = e-165 Identities = 309/636 (48%), Positives = 427/636 (66%), Gaps = 30/636 (4%) Query: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 M++LLIHSDY+E+E K+K E +E++K G+++E L FI+VE+ DE NPE ++ A Sbjct: 5 MKMLLIHSDYLEFEAKEKTKIAEE--TENLK-GKLDECLACFIAVEREDENNPEGTAIGA 61 Query: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 +EEI KVA Q+K N+ VYP+AHLSS+L+ P A+ +L + LKERG+NV +APFG+Y Sbjct: 62 VEEIEKVANQLKVNNIVVYPYAHLSSDLSSPETAVKVLKDIESILKERGYNVLRAPFGWY 121 Query: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGE-LIEVDK- 178 KAFKISCKGHPL+ELSR IV +E + EE E S +Y+L PE E +IE+++ Sbjct: 122 KAFKISCKGHPLSELSRKIVAKEEKKEEGEE----------SKFYLLNPETEEIIELNEN 171 Query: 179 -FDFTGYENLRKFVNYEIAKNRIAEK-EPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRL 236 + E L +E+ E EPPHVK + E ++ YE SDPG+ R+YPKG+L Sbjct: 172 NINIIKDEELLALAKHELGIREHKEHDEPPHVKFIKEKDICSYEEASDPGHFRWYPKGKL 231 Query: 237 IKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFA 296 ++ LL YV V+ GAM VETPIMYD +PA+ ++ ++F RQY G+K LRFA Sbjct: 232 MRDLLADYVYNLVVNMGAMPVETPIMYDLGNPAIREHADKFGERQYRFRQGNKELMLRFA 291 Query: 297 ACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDI 356 ACFGQFM+KKD + R LPL++YEL+ YSFR E+RGEL GL+RLR FTMPDMHT+ ++ Sbjct: 292 ACFGQFMMKKDMYLLPRYLPLKLYELSTYSFRYEQRGELVGLKRLRCFTMPDMHTVCLNL 351 Query: 357 EQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVK----LIGKP 412 EQA EEF+KQF ++ + + L+ Y V RFT+DF+ EH+D+ ++ K GK Sbjct: 352 EQAMEEFEKQFWECLKTGDDLNLS---YSVIFRFTKDFFDEHRDWFFKIAKEYKNKYGKD 408 Query: 413 VLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLI 472 V++E+ +R Y++ K + +D+L + TVQIDVE+A+RF I + G E YP+I Sbjct: 409 VILEILPKRKHYWVGKVDIAVIDSLGRPIENPTVQIDVESAKRFDIKVHTNEG-EIYPII 467 Query: 473 LHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGK 532 LHCSP+G+IERV+ +LEK A +G PM P+WLSPIQVRVIPV+E + DYAL VA K Sbjct: 468 LHCSPTGSIERVLCGLLEKAAIEAEKGNAPMLPVWLSPIQVRVIPVAERHYDYALKVAEK 527 Query: 533 LEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDG---KQYE 589 L IR D DD +E ++KKIR A KEW+PY+VV+G E E+ +TV RE K Y+ Sbjct: 528 LRENNIRADFDDREESVSKKIRNAGKEWVPYVVVIGDEEMESDKLTVTIREKSTLKKPYK 587 Query: 590 TR--IEELIKEIKEKTEGFPYKPRPLPLLLSKRPKF 623 + ++ELI+ IK++T +PY+P PLP+ S +PKF Sbjct: 588 EKMTLDELIERIKKETANYPYRPLPLPIRCSLQPKF 623 >sp|O27504|SYT_METTH THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7450856|pir||G69060 threonine--tRNA ligase (EC 6.1.1.3) - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622569|gb|AAB85930.1| (AE000907) threonyl-tRNA synthetase [Methanobacterium thermoautotrophicum] Length = 605 Score = 574 bits (1463), Expect = e-162 Identities = 299/623 (47%), Positives = 415/623 (65%), Gaps = 18/623 (2%) Query: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 MR+LLIHSDY++YE K+K E I ED +G E LV F +VE DE NPE V A Sbjct: 1 MRILLIHSDYLKYETKNKT-GIAEEIPEDKMQGDFRESLVVFTAVEAEDEDNPESVIENA 59 Query: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 + EI KV VKAENV +YP+AHLSS L+ P A+++L + L+ G +V + PFG+Y Sbjct: 60 VNEIIKVFNDVKAENVVIYPYAHLSSSLSSPKTAVEVLEGMESALRSEGVDVSRVPFGWY 119 Query: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFD 180 KAFKISCKGHPL+ELSR+I P+ +VEE E EE S W+IL GE ++ F+ Sbjct: 120 KAFKISCKGHPLSELSRSIRPQPPKVEE-------ESEE--SEWFIL-HRGETVKPGDFE 169 Query: 181 FTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL 240 F +L V YE+ + + +EPPHV+LM E L DYEP +D G+LR+YP+GRLI+ L Sbjct: 170 FKN-RDLENLVKYELGELESSGEEPPHVRLMREKGLADYEPSADVGHLRWYPRGRLIRDL 228 Query: 241 LEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFG 300 L YV V GAM VETPIMYD E A+ + +F RQY + K LR+A CFG Sbjct: 229 LADYVYMLVTSEGAMPVETPIMYDLEDEAIRVHAEKFGERQY-RMKNRKELMLRYACCFG 287 Query: 301 QFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAK 360 F I D+ ++++NLP +YEL+ YSFR EK+GE+ GL+RLR FTMPD+HT+ D++Q+ Sbjct: 288 AFRILSDSFLTWKNLPASIYELSTYSFRLEKKGEVVGLKRLRGFTMPDLHTVCADLDQSL 347 Query: 361 EEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQ 420 EEF +Q ++ M + + + +YEV R T DF++E+++++ E+ IGKPVL+E+ Sbjct: 348 EEFARQVEMCMRTGQDLQV---NYEVIFRATADFYEEYREWVHEVADKIGKPVLLEILPS 404 Query: 421 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA 480 R Y+I K +F +D L + TVQIDVE+ ERFGITY + + EE P+ILHCSP+G+ Sbjct: 405 RKHYWIAKMDFAAIDYLGRPIENPTVQIDVESGERFGITYVNSDEEEVNPIILHCSPTGS 464 Query: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 IERV+ ++LEK A M+E K PM PLWLSP QVRV+P++E +++YA + +L A +R Sbjct: 465 IERVICSLLEKTAIEMDE-KPPMLPLWLSPTQVRVLPIAERHMEYASDLVSELIAADVRA 523 Query: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK 600 D+DD E L KKIR A ++W+PY+VV+G E E G +TV RE G++ + ++ELI+ I+ Sbjct: 524 DLDDRSETLGKKIRNAAQDWVPYVVVIGDSEME-GKLTVNVRETGEKLQMGLDELIERIR 582 Query: 601 EKTEGFPYKPRPLPLLLSKRPKF 623 +TEG P++ PLP+ LS+R F Sbjct: 583 AETEGMPFRRLPLPVKLSERINF 605 >sp|P56881|SYT_THETH THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|6689424|emb|CAB65483.1| (AJ250953) threonyl-tRNA synthetase [Thermus thermophilus] Length = 659 Score = 184 bits (462), Expect = 3e-45 Identities = 131/396 (33%), Positives = 194/396 (48%), Gaps = 25/396 (6%) Query: 203 KEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIM 262 K+ H +L E EL +P G + + PKG +++ L ++ E+ + G V TP + Sbjct: 249 KKRDHRRLGRELELFLIDPLVGKGLVLWLPKGNVVREELMAFMREEQVRRGYQLVTTPHI 308 Query: 263 YDFEHPALEKYL-----NRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPL 317 E + ++FP + ++ Y L+ C I SYR LPL Sbjct: 309 GSLELYKTSGHYPYYAESQFPPISFKERGEEEEYLLKPMNCPHHIRIYAYRKRSYRELPL 368 Query: 318 RMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGV 377 R+ E +R EK GEL GL R+R FT D H E+ K EF L ++V + Sbjct: 369 RLAEFGTV-YRYEKAGELLGLTRVRGFTQDDAHIFCTP-EEVKGEFLGVLDLVLKVFATL 426 Query: 378 GLTPEDYEVAIRFT--------EDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKF 429 GL + +R E W + I E G +E F+ K Sbjct: 427 GLKDYRARIGVRDPKSDKYVGDEAKWALAERQIEEAAAEAGLRYTVEEGDAAFYG--PKL 484 Query: 430 EFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAIL 489 +F D L + L T+Q+D ERFG+TY ++GEE P++LH +P G++ER + ++ Sbjct: 485 DFVVKDALGREWQLGTIQVDYNLPERFGLTYVGKDGEEHRPVMLHRAPFGSLERFIGILI 544 Query: 490 EKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERL 549 E A FPLWL+P+Q V+PVSE+ DYA VAG+L+ A +R + D ER+ Sbjct: 545 EHFAG--------DFPLWLAPVQAVVVPVSEKQEDYAREVAGRLKEAGLRAEADTRPERM 596 Query: 550 NKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDG 585 +IR AE + +PYI+VVG REK G ++VRRR+ G Sbjct: 597 QARIRDAEVQKVPYILVVGEREKAEGAVSVRRRKKG 632 >sp|P18255|SYT1_BACSU THREONYL-TRNA SYNTHETASE 1 (THREONINE--TRNA LIGASE) (THRRS) >gi|68516|pir||YSBST1 threonine--tRNA ligase (EC 6.1.1.3) thrS [validated] - Bacillus subtilis >gi|143766|gb|AAA22864.1| (M36594) (thrSv) (EC 6.1.1.3) [Bacillus subtilis] >gi|1769999|emb|CAA99608.1| (Z75208) threonyl-tRNA-synthetase [Bacillus subtilis] >gi|2293284|gb|AAC00362.1| (AF008220) threonine tRNA synthetase [Bacillus subtilis] >gi|2635360|emb|CAB14855.1| (Z99118) threonyl-tRNA synthetase [Bacillus subtilis] Length = 643 Score = 182 bits (458), Expect = 8e-45 Identities = 127/407 (31%), Positives = 200/407 (48%), Gaps = 23/407 (5%) Query: 203 KEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIM 262 KE H KL E +L G + PKG I+ ++E+Y+ +K I G V TP++ Sbjct: 242 KERDHRKLGKELKLFANSQKVGQGLPLWLPKGATIRRVIERYIVDKEISLGYEHVYTPVL 301 Query: 263 YDFEHPALEKYLNRFPARQYIVLSGDKRYF-LRFAACFGQFMIKKDAIISYRNLPLRMYE 321 E + + + + + D LR C MI K I SYR LP+R+ E Sbjct: 302 GSKELYETSGHWDHYQEGMFPPMEMDNETLVLRPMNCPHHMMIYKQDIHSYRELPIRIAE 361 Query: 322 LTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTP 381 L R E G LSGL+R+R T+ D H + +Q K+EF + +L +V E GL+ Sbjct: 362 LGTMH-RYEMSGALSGLQRVRGMTLNDAHIFVRP-DQIKDEFIRTVRLIQDVYEDFGLSD 419 Query: 382 EDYEVAIRFTED---------FWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFN 432 + ++ R ED W + + + E + IG + F+ K + Sbjct: 420 YTFRLSYRDPEDTEKYFDDDEMWNKAQSMLKEAMDEIGHDYYEAEGEAAFYG--PKLDVQ 477 Query: 433 FVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQ 492 + K LSTVQ+D ERF +TY E+G++ P+++H +ER + ++E+ Sbjct: 478 VKTAIGKEETLSTVQLDFLLPERFDLTYIGEDGKQHRPVVIHRGVVSTMERFVAFLIEEH 537 Query: 493 AKLMNEGKKPMFPLWLSPIQVRVIPVSEE-YLDYALYVAGKLEGAKIRVDVDDEDERLNK 551 K P WL+P+Q +VIPVS +LDYA V +L+ +RV+VD DE++ Sbjct: 538 --------KGALPTWLAPVQFQVIPVSPAVHLDYAKKVQERLQCEGLRVEVDSRDEKIGY 589 Query: 552 KIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKE 598 KIR A+ + IPY++VVG +E ENG + VR+ + ++E +K+ Sbjct: 590 KIREAQMQKIPYMLVVGDQEAENGAVNVRKYGEQNSETISLDEFVKK 636 >sp|P18256|SYT2_BACSU THREONYL-TRNA SYNTHETASE 2 (THREONINE--TRNA LIGASE) (THRRS) >gi|68515|pir||YSBST2 threonine--tRNA ligase (EC 6.1.1.3) thrZ [validated] - Bacillus subtilis >gi|143764|gb|AAA22863.1| (M36593) threonyl-tRNA synthetase (thrS2) (EC 6.1.1.3) [Bacillus subtilis] >gi|2636292|emb|CAB15783.1| (Z99123) threonyl-tRNA synthetase [Bacillus subtilis] Length = 638 Score = 177 bits (444), Expect = 4e-43 Identities = 110/389 (28%), Positives = 202/389 (51%), Gaps = 26/389 (6%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG Y PKG+++++ LE++ E G EV TP M + + + + Y Sbjct: 263 PGMPFYLPKGQIVRNELERFSRELQTNAGYDEVRTPFMMNQRLWEQSGHWDHYRDNMYFS 322 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 D R+ ++ C G +I K+++ SYR+LP+RM E + R E G L+G+ R+R F Sbjct: 323 EVDDTRFAMKPMNCPGHMLIFKNSLYSYRDLPIRMAEFGQVH-RHEYSGALNGMLRVRTF 381 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFT-------EDFWKE 397 D H ++ +Q + E K+ +L EV G +Y V + + W+ Sbjct: 382 CQDDAHIFVRE-DQIESEIKEAIRLIDEVYRTFGF---EYSVELSTRPEDSLGDDSLWEA 437 Query: 398 HKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFG 457 + + +++ +G I F+ K +F+ D L ++ +T+Q+D + E+F Sbjct: 438 SERALARVLEELGLSYEINEGDGAFYG--PKIDFHIKDALKRSHQCATIQLDFQMPEKFD 495 Query: 458 ITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIP 517 +TY +E E+ P+++H + G+I+R ++E FP+WL+PIQV++IP Sbjct: 496 LTYINELNEKVRPVVIHRAVFGSIDRFFGILIEHYGGA--------FPVWLAPIQVQIIP 547 Query: 518 VSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTI 577 VS +LDY V +L+ A IR +D+ +E+L KIR ++ + IPY++V+G E++ + Sbjct: 548 VSHVHLDYCRKVQAELKQAGIRAGIDERNEKLGYKIRESQVQKIPYVLVLGDHEEQENAV 607 Query: 578 TVRR----REDGKQYETRIEELIKEIKEK 602 VRR + + ++T ++L+K+++ + Sbjct: 608 NVRRFGHQQNEHVPFQTFKDKLVKQVENR 636 >emb|CAB02510.1| (Z80360) Threonyl tRNA Synthetase [Bacillus subtilis] Length = 601 Score = 177 bits (444), Expect = 4e-43 Identities = 110/389 (28%), Positives = 202/389 (51%), Gaps = 26/389 (6%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG Y PKG+++++ LE++ E G EV TP M + + + + Y Sbjct: 226 PGMPFYLPKGQIVRNELERFSRELQTNAGYDEVRTPFMMNQRLWEQSGHWDHYRDNMYFS 285 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 D R+ ++ C G +I K+++ SYR+LP+RM E + R E G L+G+ R+R F Sbjct: 286 EVDDTRFAMKPMNCPGHMLIFKNSLYSYRDLPIRMAEFGQVH-RHEYSGALNGMLRVRTF 344 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFT-------EDFWKE 397 D H ++ +Q + E K+ +L EV G +Y V + + W+ Sbjct: 345 CQDDAHIFVRE-DQIESEIKEAIRLIDEVYRTFGF---EYSVELSTRPEDSLGDDSLWEA 400 Query: 398 HKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFG 457 + + +++ +G I F+ K +F+ D L ++ +T+Q+D + E+F Sbjct: 401 SERALARVLEELGLSYEINEGDGAFYG--PKIDFHIKDALKRSHQCATIQLDFQMPEKFD 458 Query: 458 ITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIP 517 +TY +E E+ P+++H + G+I+R ++E FP+WL+PIQV++IP Sbjct: 459 LTYINELNEKVRPVVIHRAVFGSIDRFFGILIEHYGGA--------FPVWLAPIQVQIIP 510 Query: 518 VSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTI 577 VS +LDY V +L+ A IR +D+ +E+L KIR ++ + IPY++V+G E++ + Sbjct: 511 VSHVHLDYCRKVQAELKQAGIRAGIDERNEKLGYKIRESQVQKIPYVLVLGDHEEQENAV 570 Query: 578 TVRR----REDGKQYETRIEELIKEIKEK 602 VRR + + ++T ++L+K+++ + Sbjct: 571 NVRRFGHQQNEHVPFQTFKDKLVKQVENR 599 >sp|Q55806|SYT_SYNY3 THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437520|pir||S76615 hypothetical protein - Synechocystis sp. (strain PCC 6803) >gi|1001722|dbj|BAA10559.1| (D64004) threonyl-tRNA synthetase [Synechocystis sp.] Length = 603 Score = 176 bits (442), Expect = 6e-43 Identities = 131/491 (26%), Positives = 238/491 (47%), Gaps = 47/491 (9%) Query: 127 CKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFDFTGYEN 186 C G P E + I P+ +E V A YW + ++ + T +E Sbjct: 134 CAG-PHLESTADINPKAIALESVAGA----------YW---RGDANKAQLQRIYGTAWET 179 Query: 187 LRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVT 246 + Y+ K +++ H KL E L + PG + PKG +I+++LE ++ Sbjct: 180 PEQLAEYQRRKEEALKRD--HRKLGKELGLFIFADPVGPGLPLWTPKGTIIRTILEDFLK 237 Query: 247 EKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKR-------YFLRFAACF 299 ++ I+ G + V TP + + + ++ + +++ + L+ C Sbjct: 238 QEQIKRGYLPVVTPHIARVDLFKQSGHWQKYQEDMFPMMAESPEEAAQEMGFVLKPMNCP 297 Query: 300 GQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQA 359 I K + SYR+LPLR+ E +R E+ GEL GL R+R FT+ D H +Q Sbjct: 298 FHIQIYKSELRSYRDLPLRLAEFGTV-YRYEQSGELGGLTRVRGFTVDDSHLFVTP-DQL 355 Query: 360 KEEFKKQFKLSMEVLEGVGLTPEDYEVAIRF----------TEDFWKEHKDFIVELVKLI 409 EEF L + V + + L ++++ + F +++ W++ + I V+ + Sbjct: 356 DEEFLSVVDLILTVFKSLQL--KNFKARLSFRDPESDKYIGSDEAWEKAQGAIRRAVQEL 413 Query: 410 GKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKY 469 + F+ K +F F D L++ L TVQ+D ERF + Y +G + Sbjct: 414 EMDYFEAPGEAAFYG--PKLDFIFQDALEREWQLGTVQVDYNLPERFDLEYIAADGSRQR 471 Query: 470 PLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYV 529 P+++H +P G++ER++ ++E+ A FPLWL+PIQVR++ VS+E+L +A + Sbjct: 472 PVMIHRAPFGSLERLIGILIEEYAG--------DFPLWLAPIQVRLLAVSDEFLPFAQSI 523 Query: 530 AGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYE 589 +++ +R +VD +RL K IR AEK+ IP + V+GA+E E + +R R G+ E Sbjct: 524 VQQMQSLGLRAEVDTSGDRLGKMIRNAEKQKIPVMAVIGAKEVEANALNIRTRASGELGE 583 Query: 590 TRIEELIKEIK 600 + E++++++ Sbjct: 584 IPVTEVLEKLQ 594 >gb|AAG20043.1| (AE005084) threonyl-tRNA synthetase; ThrS [Halobacterium sp. NRC-1] Length = 650 Score = 174 bits (436), Expect = 3e-42 Identities = 125/424 (29%), Positives = 211/424 (49%), Gaps = 24/424 (5%) Query: 194 EIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYG 253 E R KE H KL E +L + PG Y+P G+ + L +YV E G Sbjct: 240 EYLARREEAKERDHRKLGREMDLFSIPDVTGPGLPLYHPNGKTVLRELSEYVRGLNSEMG 299 Query: 254 AMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYR 313 EVETP ++ E + + + +++ D+ Y L+ C G I + SYR Sbjct: 300 YDEVETPHLFRTELWKQSGHYDNYVDDMFLLDVNDEEYGLKPMNCPGHATIFNEGSWSYR 359 Query: 314 NLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEV 373 +LP+R +E + +R+E+RGELSGL R+ AFT+ D H A+ +Q + E ++ EV Sbjct: 360 DLPVRYFEDGKV-YRKEQRGELSGLSRVWAFTIDDGHVFAR-ADQIEAEVRRLMDTITEV 417 Query: 374 LEGVGLTPEDYEVAIRF-------TEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFI 426 L+ L DYEVA+ ++D W++ + + E++ G +E F+ Sbjct: 418 LDTFDL---DYEVALATRPEKSVGSDDIWEQSERQLREVLDQQGVDYDVEPGDGAFYG-- 472 Query: 427 LKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMY 486 K +F F D L ++ TVQ+D +RF + Y + + P+++H + G+ ER Sbjct: 473 PKIDFGFEDALGRSWDGPTVQLDFNMPDRFDLEYTGSDNDAHQPVMIHRALYGSYERFFM 532 Query: 487 AILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDED 546 ++E FP WL+P QVR++PV+++ L YA V +L RV+V+D D Sbjct: 533 VLIEHY--------NGRFPTWLAPEQVRILPVTDDNLGYAHRVKNEL--GDYRVEVEDRD 582 Query: 547 ERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKEKTEGF 606 + KKI++A + +PY++VVG E++ ++VR R++ + + E + + +G Sbjct: 583 WTVGKKIQQAHDDNVPYMLVVGDDEEDADAVSVRDRQEREGKGVDLAEFRAHLDSEVDGK 642 Query: 607 PYKP 610 +P Sbjct: 643 RTEP 646 >pir||B75317 threonyl-tRNA synthetase - Deinococcus radiodurans (strain R1) >gi|6459875|gb|AAF11630.1|AE002044_3 (AE002044) threonyl-tRNA synthetase [Deinococcus radiodurans] Length = 649 Score = 173 bits (434), Expect = 5e-42 Identities = 127/412 (30%), Positives = 204/412 (48%), Gaps = 24/412 (5%) Query: 203 KEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIM 262 K H KL E EL +P G + P G +++ L ++ E+ + G V TP + Sbjct: 245 KRRDHRKLGKELELFTIDPLVGKGLPLWLPNGTVLREELTNFMKEQQFQRGYQGVVTPNI 304 Query: 263 YDFEHPALEKYLNRFPARQYIVLSGDKR-YFLRFAACFGQFMIKKDAIISYRNLPLRMYE 321 + + + + Q+ + D+ Y L+ C I SYR+LP+R+ E Sbjct: 305 GNLDLYRTSGHYPYYSESQFNPIQVDEEEYMLKPMNCPHHVRIYASKPRSYRDLPVRLAE 364 Query: 322 LTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTP 381 +R E+ GEL+GL R+R FT D H + +Q K EF L++ VL+ G+T Sbjct: 365 FGTV-YRYEQSGELNGLTRVRGFTQDDAHIFCRP-DQLKREFLDVLDLTVLVLKTFGMTD 422 Query: 382 EDYEVAIRFTEDF--------WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNF 433 + V +R E W + I+E V+ +G P IE F+ K +F Sbjct: 423 VRFRVGVRDPESDKYVGDEQNWALAERQIIEAVEEVGLPYTIEPGDAAFYG--PKLDFVV 480 Query: 434 VDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQA 493 D L + L T+Q+D ERF I+Y E+G+E P+++H +P G+IER ++E A Sbjct: 481 KDVLGREWQLGTIQVDYNLPERFDISYTGEDGQEHRPIMIHRAPFGSIERFTGILIEHYA 540 Query: 494 KLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKI 553 FPLWL+P QV +IP+++ + YA + +L A +R +VDD R+ K+ Sbjct: 541 G--------DFPLWLAPRQVMIIPIADRHNAYAEELREELHRAGLRAEVDDSSNRMQAKV 592 Query: 554 RRAEKEWIPYIVVVGAREKENGTITVRRR--EDGKQYE-TRIEELIKEIKEK 602 R AE IP +++VG +E+E ++VR R E K+ + + +EL E+ E+ Sbjct: 593 RDAELHKIPVMLIVGDKEQEAREVSVRERTGEGTKERKGVKFDELKAELLER 644 >dbj|BAB06860.1| (AP001517) threonyl-tRNA synthetase 1 [Bacillus halodurans] Length = 645 Score = 171 bits (428), Expect = 3e-41 Identities = 123/413 (29%), Positives = 202/413 (48%), Gaps = 23/413 (5%) Query: 203 KEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIM 262 KE H KL E + G + PKG I+ ++E+Y+ +K + G V TP++ Sbjct: 244 KERDHRKLGKELGIFALSQKVGQGLPLWLPKGATIRRIIERYIVDKEEKLGYQHVYTPVL 303 Query: 263 YDFEHPALEKYLNRFPARQYIVLSGD-KRYFLRFAACFGQFMIKKDAIISYRNLPLRMYE 321 E + + + + + + + LR C M+ K + SYR LPLR+ E Sbjct: 304 ASSELYKTSGHWDHYKDDMFPTMEMENEELVLRPMNCPHHMMVYKTEMRSYRQLPLRIAE 363 Query: 322 LTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTP 381 L R E G +SGL+R+R T+ D H + +Q K+EF + +L V E GL Sbjct: 364 LG-LMHRYEMSGAVSGLQRVRGMTLNDAHIFCRP-DQIKDEFVRVVRLIQAVYEDFGLKN 421 Query: 382 EDYEVAIRFTED---------FWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFN 432 + ++ R ED W + + + E + + + E + FY K + Sbjct: 422 YSFRLSYRDPEDKEKYFDDDNMWNKAQGMLKEAMDEL-ELEYFEAEGEAAFYGP-KLDVQ 479 Query: 433 FVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQ 492 L K LSTVQ+D ERF +TY E+G+ P+++H +ER + +LE+ Sbjct: 480 VRTALGKDETLSTVQLDFLLPERFDLTYVGEDGQPHRPVVVHRGVVSTMERFVAFLLEEY 539 Query: 493 AKLMNEGKKPMFPLWLSPIQVRVIPVSEE-YLDYALYVAGKLEGAKIRVDVDDEDERLNK 551 K FP WL+P+QV+VIPVS E +L+YA V L+ A IRV++D+ DE++ Sbjct: 540 --------KGAFPTWLAPVQVQVIPVSPEAHLEYAKNVQETLQQAGIRVEIDERDEKIGY 591 Query: 552 KIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKEKTE 604 KIR A+ + IPY++V+G +E E + VR+ + ++E ++ + + + Sbjct: 592 KIREAQMQKIPYMLVLGDKEVEANGVNVRKYGEKDSSSMGLDEFVRHVAREAK 644 >gb|AAK42622.1| Threonyl-tRNA synthetase (thrS) [Sulfolobus solfataricus] Length = 545 Score = 165 bits (413), Expect = 2e-39 Identities = 119/445 (26%), Positives = 223/445 (49%), Gaps = 28/445 (6%) Query: 169 PEGELIEVDKFDFTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNL 228 P + + + F E L+ ++++ + K +E H + + +L + + G + Sbjct: 107 PNEQYVRIRGVAFETEEQLKDYLSW-LEK----AEETDHRLIGEKLDLFSFHEEAGSGLV 161 Query: 229 RYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGD 288 ++PKG+ I++ L ++ E G EV T ++ + + + + + + Sbjct: 162 LFHPKGQTIRNELIAFMREINDSMGYQEVYTSHVFKTDIWKISGHYTLYRDKLIVFNMEG 221 Query: 289 KRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPD 348 Y ++ C +I K +YR+LP+R E + +R EK+GEL GL R+R F D Sbjct: 222 DEYGVKPMNCPAHILIYKSKPRTYRDLPIRFSEFG-HVYRWEKKGELYGLLRVRGFVQDD 280 Query: 349 MHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRF-------TEDFWKEHKDF 401 H ++ +Q +EE K ++EV G +D + + +++ W++ + Sbjct: 281 GHIFLRE-DQLREEIKMLISKTVEVWHKFGFKDDDIKPYLSTRPDESIGSDELWEKATNA 339 Query: 402 IVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYY 461 ++ ++ G I+ K+ FY K +F D+L + LST+Q+D ERF + Y Sbjct: 340 LISALQESGLKFGIKE-KEGAFYGP-KIDFEIRDSLGRWWQLSTIQVDFNLPERFKLEYI 397 Query: 462 DENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEE 521 D++G +K P+++H + G+I+R + +LE K P WLS +QVRV+P+++E Sbjct: 398 DKDGIKKRPVMVHRAIYGSIDRFVAILLEHF--------KGKLPTWLSSVQVRVLPITDE 449 Query: 522 YLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRR 581 +YA V + +IR ++D E L+K+I+ A + +PYI++VG +E GT+TVR Sbjct: 450 VNEYAEKVLNDMRKRRIRAEIDYAGETLSKRIKNAYDQGVPYILIVGKKEASEGTVTVRA 509 Query: 582 RED----GKQYETRIEELIKEIKEK 602 R + ++E +E LI EI ++ Sbjct: 510 RGNIEVRNVKFEKFLELLITEIAQR 534 >sp|Q9WZJ9|SYT_THEMA THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437519|pir||G72339 threonine--tRNA ligase (EC 6.1.1.3) - Thermotoga maritima (strain MSB8) >gi|4981265|gb|AAD35821.1|AE001744_11 (AE001744) threonyl-tRNA synthetase [Thermotoga maritima] Length = 640 Score = 163 bits (408), Expect = 6e-39 Identities = 120/450 (26%), Positives = 216/450 (47%), Gaps = 31/450 (6%) Query: 162 SYWYILTPEGELIEVDKFDFTGYENLRKFVNY-EIAKNRIAEKEPPHVKLMLEHELVDYE 220 +YW L V F E+L ++ + E A+ R K P ++L + + +Y Sbjct: 201 AYWRGSEKNPMLTRVYGTAFAKKEDLDNYLKFLEEAQRRDHRKLGPQLELFMLN--TEYA 258 Query: 221 PGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPAR 280 PG + PKG ++ + L ++ E E G E+ TP++ + + + + + + Sbjct: 259 PGMP----FFLPKGVVVLNELMKFSRELHRERGYQEIFTPLIMNEQLWKISGHWDHYAEN 314 Query: 281 QYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRR 340 Y + ++RY ++ C G ++ K +SYR+LPLR +E R R E+ G L GL R Sbjct: 315 MYFIEKDEERYAVKPMNCPGHILVYKSRTVSYRDLPLRFFEFGRVH-RYERSGVLHGLMR 373 Query: 341 LRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLT--------PEDYEVAIRFTE 392 +R+FT D H +Q +EE L + G T PED+ E Sbjct: 374 VRSFTQDDAHIFCTP-DQIEEEILGVLDLINTIYSQFGFTYRVELSTMPEDH----MGDE 428 Query: 393 DFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVEN 452 W++ + ++ G + + F+ K +F+ D++ + +T+Q+D Sbjct: 429 AIWEKATTALKNALERAGLSYKVNEGEGAFYG--PKIDFHIRDSIGREWQCATIQLDFMM 486 Query: 453 AERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQ 512 E+F +TY + +E +++H + G++ER ++E A FP WL+PIQ Sbjct: 487 PEKFNVTYIGPDNKEHRAVMIHRAIYGSLERFFGILIEHFAGA--------FPTWLAPIQ 538 Query: 513 VRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREK 572 V VIP+SE++ D A VA ++ RV D+ E L +IR+A+ + IPY++++G +E Sbjct: 539 VAVIPISEKHNDGAEKVARRISQEGFRVFFDNRRETLGYRIRQAQTQKIPYMIIIGDKEL 598 Query: 573 ENGTITVRRREDGKQYETRIEELIKEIKEK 602 E+G I+VR R + + E ++ ++ + Sbjct: 599 ESGKISVRTRTGKEIKDVDPEHFVETLRNE 628 >sp|O05947|SYT_RICPR THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437518|pir||E71733 threonine--tRNA ligase (EC 6.1.1.3) - Rickettsia prowazekii >gi|2073475|emb|CAA72469.1| (Y11780) threonyl-tRNA synthetase [Rickettsia prowazekii] >gi|3860783|emb|CAA14684.1| (AJ235270) THREONYL-TRNA SYNTHETASE (thrS) [Rickettsia prowazekii] Length = 635 Score = 162 bits (407), Expect = 8e-39 Identities = 119/415 (28%), Positives = 206/415 (48%), Gaps = 27/415 (6%) Query: 201 AEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETP 260 AEK H K+ E +L ++ + G + ++ KG I + +EQY+ +K+ + G +EV+TP Sbjct: 238 AEKRD-HRKIGKELDLFHFQEEAQ-GMVFWHDKGWSIYNTIEQYIRKKIRKNGYIEVKTP 295 Query: 261 IMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMY 320 ++ D + +F + + + DK L+ C I K I SYR+LPLRM Sbjct: 296 VLVDKSLWEASGHWAKFRCDMFTLETDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMS 355 Query: 321 ELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLT 380 E R E G L GL R+R+ D H + EQ +E + KL EV + G T Sbjct: 356 EFG-LCHRNEASGALHGLMRVRSLVQDDAHIFCAE-EQITDETVRFCKLLTEVYKDFGFT 413 Query: 381 PEDYEVAIRFTE---------DFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEF 431 ++ ++F++ + W + ++ + ++ G + + F+ K EF Sbjct: 414 ----DIKVKFSDRSEICAGNDEVWDKAENALKTAIEKAGFIYTLNPGEAAFYG--PKIEF 467 Query: 432 NFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEK 491 D + + T+Q+D ER Y +GE+K P++LH + G++ER + ++E+ Sbjct: 468 VLTDAIGRQWQCGTLQMDFVLPERLDANYIAASGEKKRPVMLHRAILGSLERFIGILIEE 527 Query: 492 QAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNK 551 A GK FP+WL+P+QV + ++ + DYAL V L +R D++ +++N Sbjct: 528 YA-----GK---FPIWLAPVQVAIATITNDLNDYALEVQKTLIDNGVRTDINISPDKINY 579 Query: 552 KIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKEKTEGF 606 KIR + IP I V+G +E+ N +T+R+ +Q IE+LI IK++ + Sbjct: 580 KIREFSNQKIPIIAVIGKQEQANKQVTIRKLGTIEQEILSIEQLIAMIKKENSNY 634 >sp|O67583|SYT_AQUAE THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437523|pir||F70444 threonine--tRNA ligase (EC 6.1.1.3) - Aquifex aeolicus >gi|2984004|gb|AAC07549.1| (AE000750) threonyl-tRNA synthetase [Aquifex aeolicus] Length = 638 Score = 161 bits (404), Expect = 2e-38 Identities = 114/409 (27%), Positives = 202/409 (48%), Gaps = 24/409 (5%) Query: 203 KEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIM 262 K+ H +L E E + G + + P+G + + +LE Y+ E+ ++ G V TP + Sbjct: 240 KKRDHRRLGKELEFFTIDDNVGAGLILWLPRGAIYRKVLEDYLREEHLKRGYQLVYTPHV 299 Query: 263 YDFEHPALEKYLNRFPARQYIVLSGDKR-YFLRFAACFGQFMIKKDAIISYRNLPLRMYE 321 + +L + + + D+ Y+++ C I K SY+ LPL+++E Sbjct: 300 GKSKLWETSGHLECYKQNMFPSMKIDEEEYYVKPMNCPFHIAIYKSRTRSYKELPLKLFE 359 Query: 322 LTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGL-- 379 L +R E G L GL R+R FT D H + EQ + ++ ++ L+ G Sbjct: 360 LGTV-YRYELSGVLHGLLRVRGFTQDDAHIVCTP-EQVNDVIRETLDFALSTLKDFGFNE 417 Query: 380 ------TPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNF 433 T +Y + +++ W+ ++ + + ++ +G I+ F+ K + Sbjct: 418 FKIYLSTRPEYSIG---SDEQWEVSQNALKKAIEDLGYEYEIDEGGGAFYG--PKIDVKI 472 Query: 434 VDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQA 493 D + + LST+Q D ERF +TY + ++ P ++H + G+IER +LE A Sbjct: 473 RDAIGRMWQLSTIQFDFNLPERFDMTYVGPDNKKHRPYMIHRALLGSIERFTGILLEHYA 532 Query: 494 KLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKI 553 L+ P+WLSP QV +IP+++ + +YA V L+ IR ++D +ER+N KI Sbjct: 533 GLL--------PIWLSPTQVMIIPIADRHHEYAKKVYEFLKENGIRAEMDLREERMNAKI 584 Query: 554 RRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKEK 602 R AE + IP I+VVG RE +N T++VR +++G + + + IKEK Sbjct: 585 RDAELKKIPVILVVGDREAQNNTVSVRTKKEGNLGSMELNKFLDWIKEK 633 >pir||A82479 threonyl-tRNA synthetase VCA0287 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9657684|gb|AAF96196.1| (AE004368) threonyl-tRNA synthetase [Vibrio cholerae] Length = 642 Score = 160 bits (400), Expect = 5e-38 Identities = 108/381 (28%), Positives = 187/381 (48%), Gaps = 17/381 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++ G I LE ++ +K+ EYG EV+ P+M D + +++ + Sbjct: 262 PGMVFWHHNGWSIFRDLEIFIRQKLNEYGYQEVKGPLMMDRVLWERSGHWDKYADAMFTT 321 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S ++ Y ++ C G I + SYR+LPLRM E R E G L G+ R+R F Sbjct: 322 SSENREYAIKPMNCPGHIQIFNQGLKSYRDLPLRMAEFGS-CHRNEPSGSLHGIMRVRGF 380 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H + +Q ++E K+ + G +++ R +++ W + + Sbjct: 381 TQDDAHIFCTE-DQIQQEVTSCIKMVYDTYTTFGFQNIVVKLSTRPEKRVGSDEIWDKSE 439 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 +++ +K + P I+ + F+ K EF D LD+A TVQ+D R G T Sbjct: 440 QALIDSLKAMDIPFEIQEGEGAFYG--PKIEFTLYDCLDRAWQCGTVQLDFNLPTRLGAT 497 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y E+ E P+++H + G++ER + ++E+ A FP WL+P Q V+ ++ Sbjct: 498 YVGESNERLIPVMIHRAILGSLERFIGILIEEYAGF--------FPTWLAPEQAVVVNIT 549 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 ++ DYA VA KL+ IR D +E++ KIR + +PY++V G +E E G I V Sbjct: 550 DKQADYAHEVAQKLQKCGIRAKADLRNEKIGFKIREHTLKRVPYMLVCGDQEMEAGEIAV 609 Query: 580 RRREDGKQYETRIEELIKEIK 600 R R+ + ++++ I I+ Sbjct: 610 RTRKGKDLGKFKLDDFIAHIQ 630 >pir||H82878 threonyl-tRNA synthetase UU534 [imported] - Ureaplasma urealyticum >gi|6899538|gb|AAF30947.1|AE002152_6 (AE002152) threonyl-tRNA synthetase [Ureaplasma urealyticum] Length = 580 Score = 157 bits (392), Expect = 4e-37 Identities = 123/453 (27%), Positives = 214/453 (47%), Gaps = 29/453 (6%) Query: 162 SYWYILTPEGELIEVDKFDFTGYENLRKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEP 221 SYW +LI + F+ + L ++ K+ KE H K+ + L + Sbjct: 140 SYWRGDANNDQLIRIYGVAFSDQDQLDAYL-----KDLQERKERDHRKIGKDLNLFTFNN 194 Query: 222 GSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQ 281 + G + P G +IK+ +++++ E ++ V TPI+ + + + + Sbjct: 195 LAGQGLPIWLPNGTIIKNQVQKFINEVEFQFNFDTVITPILGSIDLYKTSGHWDHYKDNI 254 Query: 282 YIVLSGDKRYF-LRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRR 340 + + D LR C ++ + + SYR+LP+R+ E + R E G L+G R Sbjct: 255 FSPVQIDNEILVLRPMTCPHHTLVYSNELRSYRSLPIRLSEHS-ILHRYESSGGLTGFER 313 Query: 341 LRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTED------- 393 +R + D H + +Q + E FK+ E EG+G+ + +++ D Sbjct: 314 VREMILEDCHVFCR-FDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPNDKEKYYDD 372 Query: 394 --FWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVE 451 W++ ++ + +++K P EM + FY K +F L++ +ST+Q+D Sbjct: 373 PQMWEQSQNVLRKMLKDHNIPYK-EMVGEAAFYGP-KIDFQVKTVLNRIITVSTIQLDFL 430 Query: 452 NAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPI 511 RF +TY +E+ E+ P+++H G ER++ +LE+ K + PLWLSPI Sbjct: 431 LPNRFNLTYINESNEQSVPVMIHIGIIGTYERLLAILLEQ--------TKGILPLWLSPI 482 Query: 512 QVRVIPVSEE-YLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAR 570 QV +IPV+E + DY + KL IR +VD +ERL+KKIR A+ + IPY +V+G Sbjct: 483 QVVIIPVNENLHTDYVKELNIKLRKHLIRSNVDLRNERLSKKIREAQIQKIPYQIVIGDE 542 Query: 571 E-KENGTITVRRREDGKQYETRIEELIKEIKEK 602 E K N +T R K I + I ++ K Sbjct: 543 EIKNNKMVTYRCYGSEKTTTVSITDFINMLENK 575 >sp|O51662|SYT_BORBU THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437524|pir||G70189 threonine--tRNA ligase (EC 6.1.1.3) thrZ - Lyme disease spirochete >gi|2688663|gb|AAC67076.1| (AE001172) threonyl-tRNA synthetase (thrZ) [Borrelia burgdorferi] Length = 581 Score = 155 bits (388), Expect = 1e-36 Identities = 130/479 (27%), Positives = 217/479 (45%), Gaps = 27/479 (5%) Query: 128 KGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFDFTGYENL 187 K H +L R P + ++ K +YW L + + + L Sbjct: 114 KSHNFVDLCRG--PHVENMNKIDPKAFKLTSIAGAYWRGSEKNPMLTRIYGTLWNNEKEL 171 Query: 188 RKFVNYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTE 247 R ++N R K+ H KL E +L PG + ++P G I++L+E + E Sbjct: 172 RSYLNL-----REEIKKRDHRKLGKELDLFSIHEEIGPGLVFFHPNGAKIRALIEDFWRE 226 Query: 248 KVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKR-YFLRFAACFGQFMIKK 306 + + G + TP + +L+ + + + DK Y+L+ C I Sbjct: 227 EHSKNGYDILFTPHIGKSWLWQTSGHLDFYKDSMFEKIEMDKSDYYLKPMNCPFHIAIYN 286 Query: 307 DAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQ 366 SYR+LP R EL +R EK G L G+ R R FT D H + Q +E K+ Sbjct: 287 TGKHSYRDLPFRWAELGTV-YRYEKIGALHGMMRARGFTQDDAHIICTH-SQVLDEIKEV 344 Query: 367 FKLSMEVLEGVGLT-PEDY-----EVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQ 420 + ++ + G + P+ Y + ++ D W+ + E + P I+ Sbjct: 345 LRFAIYMWSKFGFSNPKAYLSTKPDKSVGNDSD-WEMSLKVLEETLSDFEVPYEIDKGGG 403 Query: 421 RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGA 480 F+ K + VD+L++ +ST+Q D ERF +TY E+G+EK P ++H + G+ Sbjct: 404 AFYG--PKIDLKIVDSLEREWQMSTIQFDFNLPERFNMTYTAEDGKEKRPFMIHRALLGS 461 Query: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 IER ++E FPLWLSP+QV +IPV+ DYA+ V K + IR+ Sbjct: 462 IERFFGILVEHYGGA--------FPLWLSPVQVVIIPVNNIVEDYAIKVFNKFKNEGIRI 513 Query: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEI 599 +D+ R+N KIR + + IPY+ ++G RE I++R R + + +++E +K I Sbjct: 514 KLDNSSSRMNAKIREYQAKKIPYMFIIGEREATEERISIRTRTNEQINGMKLDEALKFI 572 >sp|Q9ZMV3|SYT_HELPJ THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437521|pir||G71972 threonine--tRNA ligase (EC 6.1.1.3) - Helicobacter pylori (strain J99) >gi|4154621|gb|AAD05692.1| (AE001450) THREONYL-TRNA SYNTHETASE [Helicobacter pylori J99] Length = 612 Score = 153 bits (384), Expect = 4e-36 Identities = 114/438 (26%), Positives = 210/438 (47%), Gaps = 25/438 (5%) Query: 173 LIEVDKFDFTGYENLRKFV-NYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYY 231 LI + F E L+ ++ E AK R H KL +E L ++ G + Sbjct: 190 LIRIYGIAFATKEGLKDYLFQIEEAKKR------DHRKLGVELGLFSFDDEIGAGLPLWL 243 Query: 232 PKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRY 291 PKG ++ +E +++ ++ G V+ P + + + + + + Y ++ Y Sbjct: 244 PKGARLRKRIEDLLSQALLLRGYEPVKGPEILKSDVWKISGHYDNYKENMYFTTIDEQEY 303 Query: 292 FLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHT 351 ++ C G + + A+ SYR+LPLR YE R EK G L GL R+R FT D H Sbjct: 304 GIKPMNCVGHIKVYQSALHSYRDLPLRFYEYG-VVHRHEKSGVLHGLLRVREFTQDDAHI 362 Query: 352 LAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDF-----WKEHKDFIVELV 406 EQ + E + ++++ G + E E++ R + W++ + + E + Sbjct: 363 FCS-FEQIQSEVSAILDFTHKIMQAFGFSYE-MELSTRPAKSIGDDKVWEKATNALKEAL 420 Query: 407 KLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGE 466 K I+ F+ K + D L + T+Q+D+ ERF + + +E Sbjct: 421 KEHRIDYKIDEGGGAFYG--PKIDIKITDALKRKWQCGTIQVDMNLPERFKLAFTNEYNH 478 Query: 467 EKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYA 526 + P+++H + G+ ER + +++E FP +++P Q+ +IP++EE+ +A Sbjct: 479 AEQPVMIHRAILGSFERFI--------AILSEHFGGNFPFFVAPTQIALIPINEEHHVFA 530 Query: 527 LYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGK 586 L + L+ I V+V D+++ LNKK+R AEK+ IP I+V+G E E +++R RE Sbjct: 531 LKLKEALKKCDIFVEVLDKNDSLNKKVRLAEKQKIPMILVLGNEEVETEILSIRDREKQA 590 Query: 587 QYETRIEELIKEIKEKTE 604 QY+ ++E + ++ K + Sbjct: 591 QYKMPLKEFLNMVESKMQ 608 >sp|P56071|SYT_HELPY THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437522|pir||C64535 threonine--tRNA ligase (EC 6.1.1.3) - Helicobacter pylori (strain 26695) >gi|2313207|gb|AAD07192.1| (AE000534) threonyl-tRNA synthetase (thrS) [Helicobacter pylori 26695] Length = 612 Score = 150 bits (376), Expect = 3e-35 Identities = 113/438 (25%), Positives = 209/438 (46%), Gaps = 25/438 (5%) Query: 173 LIEVDKFDFTGYENLRKFV-NYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYY 231 LI + F E L+ ++ E AK R H KL +E L ++ G + Sbjct: 190 LIRIYGIAFATKEGLKDYLFQIEEAKKR------DHRKLGVELGLFSFDDEIGAGLPLWL 243 Query: 232 PKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRY 291 PKG ++ +E +++ ++ G V+ P + + + + + + Y ++ Y Sbjct: 244 PKGARLRKRIEDLLSQALLLRGYEPVKGPEILKSDVWKISGHYDNYKENMYFTTIDEQEY 303 Query: 292 FLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHT 351 ++ C G + + A+ SYR+LPLR YE R EK G L GL R+R FT D H Sbjct: 304 GIKPMNCVGHIKVYQSALHSYRDLPLRFYEYG-VVHRHEKSGVLHGLLRVREFTQDDAHI 362 Query: 352 LAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDF-----WKEHKDFIVELV 406 EQ + E + ++++ + E E++ R + W++ + + E + Sbjct: 363 FCS-FEQIQSEVSAILDFTHKIMQAFDFSYE-MELSTRPAKSIGDDKVWEKATNALKEAL 420 Query: 407 KLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGE 466 K I+ F+ K + D L + T+Q+D+ ERF + + +E Sbjct: 421 KEHRIDYKIDEGGGAFYG--PKIDIKITDALKRKWQCGTIQVDMNLPERFKLAFTNEYNH 478 Query: 467 EKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYA 526 + P+++H + G+ ER + +++E FP +++P Q+ +IP++EE+ +A Sbjct: 479 AEQPVMIHRAILGSFERFI--------AILSEHFGGNFPFFVAPTQIALIPINEEHHVFA 530 Query: 527 LYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGK 586 L + L+ I V+V D+++ LNKK+R AEK+ IP I+V+G E E +++R RE Sbjct: 531 LKLKEALKKRDIFVEVLDKNDSLNKKVRLAEKQKIPMILVLGNEEVETEILSIRDREKQD 590 Query: 587 QYETRIEELIKEIKEKTE 604 QY+ ++E + ++ K + Sbjct: 591 QYKMPLKEFLNMVESKMQ 608 >sp|O83809|SYT_TREPA THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437525|pir||B71275 threonine--tRNA ligase (EC 6.1.1.3) (thrS) - syphilis spirochete >gi|3323150|gb|AAC65804.1| (AE001254) threonyl-tRNA synthetase (thrS) [Treponema pallidum] Length = 592 Score = 150 bits (374), Expect = 6e-35 Identities = 130/472 (27%), Positives = 209/472 (43%), Gaps = 42/472 (8%) Query: 122 AFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEEELVSYWYILTPEGELIEVDKFDF 181 AF C+G P + R I P ++ + A YW +L + + Sbjct: 125 AFTDLCRG-PHVQSMRDINPHAFKLTSIAGA----------YWRGNERGPQLTRIYGTAW 173 Query: 182 TGYENLRKFVNYEI-AKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSL 240 E+L ++ + AK R K P + L E +PG + ++P+G + Sbjct: 174 ESEEDLHTYLRMQDEAKRRDHRKLGPALGLFHLDE-------ENPGQVFWHPEGWTLYVA 226 Query: 241 LEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYF-LRFAACF 299 ++QY+ + E G EV TP + + +++ A Y+ G+KR F L+ C Sbjct: 227 IQQYLRRVMHEDGYAEVHTPFVMPQSLWERSGHWDKYRANMYLT-EGEKRSFALKPMNCP 285 Query: 300 GQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQA 359 G I K SYR+LPLR+ E + R E G L G+ R+R F D H + + A Sbjct: 286 GHVEIFKQKTRSYRDLPLRLSEFGSCT-RNEPSGSLHGVMRVRGFVQDDAHIFCTEAQIA 344 Query: 360 KEEFKKQFKLSMEVLEGVGLTPEDYEVAI------RFTEDF-WKEHKDFIVELVKLIGKP 412 E + +L V G E V R +D W + + E + G Sbjct: 345 SE-VTRFCRLLARVYADFGFAQEQIRVKFSTRPEQRIGDDATWDRAERALAEACEAAG-- 401 Query: 413 VLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVE--NAERFGITYYDENGEEKYP 470 + E ++ K EF +D L++ T+Q+D + + ER Y E+ + P Sbjct: 402 LSYEHAPGEGAFYGPKLEFALIDTLEREWQCGTIQVDYQLPSCERLNAEYVGEDNQRHMP 461 Query: 471 LILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVA 530 +ILH + G++ER + ++E FP WL+P+Q VIPV+ +L+YA +VA Sbjct: 462 VILHRTVIGSLERFIGILIEHYGGA--------FPPWLAPVQAVVIPVAPAFLEYAQHVA 513 Query: 531 GKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRR 582 +L +RV D ER+N KIR A+ + +PY+++VG RE + VR R Sbjct: 514 RELCARSLRVQADVSAERMNAKIRTAQTQKVPYLLIVGERELRAQQVAVRPR 565 >pir||T51624 threonine--tRNA ligase (EC 6.1.1.3) [imported] - Arabidopsis thaliana >gi|3617770|emb|CAA74705.1| (Y14329) threonyl-tRNA synthetase [Arabidopsis thaliana] Length = 709 Score = 149 bits (372), Expect = 1e-34 Identities = 125/454 (27%), Positives = 210/454 (45%), Gaps = 31/454 (6%) Query: 162 SYWYILTPEGELIEVDKFDFTGYENLRKFVNY-EIAKNRIAEKEPPHVKLMLEHELVDYE 220 +YW L V + + L+K++ + E AK +L H+L Sbjct: 277 AYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLLGQKQELFFSHQL---- 332 Query: 221 PGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPAR 280 PG+ + P G + + L ++ + G EV TP MY+ E + + + Sbjct: 333 ---SPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQTSGHADNYKDN 389 Query: 281 QYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRR 340 + + + L+ C G +I + + SYR LPLR+ + R E G LSGL R Sbjct: 390 MFTFNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPLRLADFG-VLHRNEASGALSGLTR 448 Query: 341 LRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAI-----RFTEDF- 394 +R F D H EQ K E + + V + G T YE+ + ++ D Sbjct: 449 VRRFQQDDAHIFCTT-EQVKGEVQGVLEFIDYVYKVFGFT---YELKLSTRPEKYLGDLE 504 Query: 395 -WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENA 453 W + + + E ++ GKP+++ F+ K + D +++ +T+Q+D + Sbjct: 505 TWDKAEADLKEAIEAFGKPLVLNEGDGAFYG--PKIDITVSDAMNRKFQCATLQLDFQLP 562 Query: 454 ERFGITYYDENGEEKY-PLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQ 512 RF + Y E+ +K P+++H + G++ER M+AIL + K GK +P W+SP Q Sbjct: 563 IRFNLEYAAEDEAKKSRPVMIHRAVLGSVER-MFAILLEHYK----GK---WPFWISPRQ 614 Query: 513 VRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREK 572 V P+SE+ YA V +++ A VD D D +++KK+R A+ YI+VVG E Sbjct: 615 AIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEA 674 Query: 573 ENGTITVRRREDGKQYETRIEELIKEIKEKTEGF 606 G ++VR R++ IE+L++E K KT F Sbjct: 675 ATGQVSVRVRDNAAHSVKSIEDLLEEFKAKTAEF 708 >pir||B81939 probable threonine--tRNA ligase (EC 6.1.1.3) NMA0929 [imported] - Neisseria meningitidis (group A strain Z2491) >gi|7379634|emb|CAB84201.1| (AL162754) putative threonyl-tRNA synthetase [Neisseria meningitidis Z2491] Length = 637 Score = 148 bits (371), Expect = 1e-34 Identities = 108/392 (27%), Positives = 192/392 (48%), Gaps = 19/392 (4%) Query: 199 RIAEKEP-PHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEV 257 RI E E H KL + +L + + PG + ++PKG + ++EQ++ +++ G EV Sbjct: 234 RIEEAEKRDHRKLGKQLDLFHLQDEA-PGMVFWHPKGWALWQVIEQHMRKELNAAGYKEV 292 Query: 258 ETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPL 317 +TP + D + + ++ S + Y ++ C G I + + SYR+LP+ Sbjct: 293 KTPQIMDKTFWEKSGHWENYKDNMFVTSSEKREYAVKPMNCPGHVQIFNNGLRSYRDLPM 352 Query: 318 RMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGV 377 R+ E R E G L GL R+R F D H + +Q E + +L + + + Sbjct: 353 RLAEFGS-CHRNEPSGALHGLMRVRGFVQDDAHIFCTE-DQIVSEARAFNELLVRIYKQF 410 Query: 378 GLTPEDYEVAIR-----FTEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFN 432 G ++++R ++D W + + + E + G E+ + FY K E++ Sbjct: 411 GFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE-WGELPGEGAFYGP-KIEYH 468 Query: 433 FVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQ 492 D L ++ T+Q+D ER Y EN + P++LH + G++ER + ++E Sbjct: 469 VRDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVMLHRAILGSLERFIGILIENH 528 Query: 493 AKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKK 552 A FPLWL+P+Q+ ++ ++E DY VA KL+ A RV++D +E++ K Sbjct: 529 AG--------SFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGFRVELDLRNEKIGYK 580 Query: 553 IRRAEKEWIPYIVVVGAREKENGTITVRRRED 584 IR + PY +VVG +EK+ + VRR+ + Sbjct: 581 IRDNSQYRFPYQIVVGDKEKQENKVAVRRKAE 612 >pir||E81167 threonyl-tRNA synthetase NMB0720 [imported] - Neisseria meningitidis (group B strain MD58) >gi|7225946|gb|AAF41133.1| (AE002426) threonyl-tRNA synthetase [Neisseria meningitidis MC58] Length = 637 Score = 148 bits (369), Expect = 2e-34 Identities = 107/392 (27%), Positives = 192/392 (48%), Gaps = 19/392 (4%) Query: 199 RIAEKEP-PHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEV 257 RI E E H KL + +L + + PG + ++PKG + ++EQ++ +++ G EV Sbjct: 234 RIEEAEKRDHRKLGKQLDLFHLQDEA-PGMVFWHPKGWALWQVIEQHMRKELNAAGYKEV 292 Query: 258 ETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPL 317 +TP + D + + + ++ S + Y ++ C G I + + SYR+LP+ Sbjct: 293 KTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCPGHVQIFNNGLRSYRDLPM 352 Query: 318 RMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGV 377 R+ E R E G L GL R+R F D H + +Q E + +L + + + Sbjct: 353 RLAEFGS-CHRNEPSGALHGLMRVRGFVQDDAHIFCTE-DQIVSEARAFNELLIRIYKQF 410 Query: 378 GLTPEDYEVAIR-----FTEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFN 432 G ++++R ++D W + + + E + G E+ + FY K E++ Sbjct: 411 GFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE-WGELPGEGAFYGP-KIEYH 468 Query: 433 FVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQ 492 D L ++ T+Q+D ER Y EN + P++LH + G++ER + ++E Sbjct: 469 VRDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVMLHRAILGSLERFIGILIENH 528 Query: 493 AKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKK 552 A FPLWL+P+Q+ ++ ++E DY VA KL+ A R ++D +E++ K Sbjct: 529 AG--------SFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGFRAELDLRNEKIGYK 580 Query: 553 IRRAEKEWIPYIVVVGAREKENGTITVRRRED 584 IR + PY +VVG +EK+ + VRR+ + Sbjct: 581 IRDNSQYRFPYQIVVGDKEKQENKVAVRRKAE 612 >gb|AAF53166.1| (AE003635) CG5353 gene product [alt 1] [Drosophila melanogaster] >gi|7297922|gb|AAF53167.1| (AE003635) CG5353 gene product [alt 2] [Drosophila melanogaster] Length = 690 Score = 147 bits (368), Expect = 3e-34 Identities = 115/416 (27%), Positives = 199/416 (47%), Gaps = 40/416 (9%) Query: 207 HVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE 266 H K+ E EL + S PG+ + P+G I + L ++ + + G EV +P +Y+ + Sbjct: 292 HRKIGREQELFFFHELS-PGSCFFQPRGAHIYNTLMGFIKAEYRKRGFQEVISPNIYNAK 350 Query: 267 HPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYS 326 + + + + +++ L+ C G +I + S+R LPLRM + Sbjct: 351 LWMTSGHWQHYAENMFSFEAEKEKFALKPMNCPGHCLIFDNRNRSWRELPLRMADFG-VL 409 Query: 327 FRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGV--------- 377 R E G L+GL R+R F D H A E+ K + K +E L+ V Sbjct: 410 HRNELSGALTGLTRVRRFQQDDAHIFC-----APEQIKSEMKGCLEFLKYVYTIFGFSFQ 464 Query: 378 ---GLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQR---FFYFILKFEF 431 P++Y + + W + + + E + G P WK+ ++ K + Sbjct: 465 LVLSTRPDNYLGEL----EQWNDAEKALAESLNEFGMP-----WKENPGDGAFYGPKIDI 515 Query: 432 NFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEK 491 +D L +A +T+Q+D + RF ++Y ++GE+K P+I+H + G++ER++ + E Sbjct: 516 TIMDALKRAHQCATIQLDFQLPIRFNLSYIADDGEKKRPVIIHRAILGSVERMIAILTEN 575 Query: 492 QAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVD-DEDERLN 550 A GK +P WLSP QV V+PV Y YA V +L A + D D + +N Sbjct: 576 FA-----GK---WPFWLSPRQVMVVPVGPAYDQYAQSVRDQLHDAGFMSEADCDAGDTMN 627 Query: 551 KKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKEKTEGF 606 KKIR A+ +I+VVG +E+ + T+ VR R++ E + ELI ++++ + F Sbjct: 628 KKIRNAQLAQFNFILVVGDKERSSNTVNVRTRDNKVHGEVSVAELITKLQKIRDEF 683 >sp|O07151|SYT_MYCLE THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|2193902|emb|CAB09620.1| (Z96801) ThrS [Mycobacterium leprae] Length = 702 Score = 147 bits (368), Expect = 3e-34 Identities = 118/424 (27%), Positives = 198/424 (45%), Gaps = 35/424 (8%) Query: 207 HVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE 266 H KL +E +L + G ++PKG +++ +E+Y K IE G V TP + + Sbjct: 280 HRKLGIELDLFSFPDEIGSGLAIFHPKGSIVRREMEEYSRRKHIEAGYQFVNTPHITKAQ 339 Query: 267 --HPA--LEKYLNR-FPARQYIVLSGD--------KRYFLRFAACFGQFMIKKDAIISYR 313 H + L+ Y FP D + Y+L+ C +I SYR Sbjct: 340 LFHTSGHLDWYAEGIFPPMHLDAEHNDDGTVRKPGQDYYLKPMNCPMHTLIFSSRGRSYR 399 Query: 314 NLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEV 373 LPLR++E +R EK G + GL R R FTM D H EQ E + +++ Sbjct: 400 ELPLRLFEFGTI-YRYEKSGVVHGLTRARGFTMDDSHIFCTR-EQLHCELASLLRFVLDL 457 Query: 374 LEGVGLTPEDYEVAIRFTEDF------WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFIL 427 L GL E++ + E F W+E + E+ + P++ + F+ Sbjct: 458 LGDYGLEDFYLELSTKDPEKFVGSEEIWEEATAALAEVAENSTLPLVPDPGGAAFYG--P 515 Query: 428 KFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYA 487 K D L ++ +ST+Q+D ERF + Y +G + P+++H + G+IER Sbjct: 516 KISVQVRDALGRSWQMSTIQVDFNFPERFALEYTSADGTRQRPVMIHRALFGSIERFFGI 575 Query: 488 ILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDE 547 + E A FP WL+PIQV IPV+ E++ Y VA +L+ +R +VD D+ Sbjct: 576 LTEHYAGA--------FPAWLAPIQVVGIPVTGEHVSYLEEVAAQLKSCGVRTEVDVSDD 627 Query: 548 RLNKKIRRAEKEWIPYIVVVGAREKENGTITV----RRREDGKQYETRIEELIKEIKEKT 603 R+ KKI R + +P++++ G R+ G+++ R + +G ++ +E ++ I ++ Sbjct: 628 RMAKKIVRHTNQKVPFMLLAGDRDVRTGSVSFRFGDRTQINGVARDSAVEAIVCWIVDRE 687 Query: 604 EGFP 607 FP Sbjct: 688 NDFP 691 >dbj|BAA15486.1| (D90813) Threonine--tRNA ligase (EC 6.1.1.3) [Escherichia coli] Length = 628 Score = 146 bits (366), Expect = 5e-34 Identities = 104/383 (27%), Positives = 180/383 (46%), Gaps = 17/383 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++ G I LE +V K+ EY EV+ P M D + + + + Sbjct: 247 PGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTT 306 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S ++ Y ++ C G I + SYR+LPLRM E R E G L GL R+R F Sbjct: 307 SSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS-CHRNEPSGSLHGLMRVRGF 365 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H + EQ ++E +L ++ G +++ R +++ W + Sbjct: 366 TQDDAHIFCTE-EQIRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAE 424 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 + ++ P ++ + F+ K EF D LD+A TVQ+D R + Sbjct: 425 ADLAVALEENNIPFEYQLGEGAFYG--PKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSAS 482 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y E+ E K P+++H + G++ER + + E+ A FP WL+P+QV ++ ++ Sbjct: 483 YVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGF--------FPTWLAPVQVVIMNIT 534 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 + +Y + KL A IRV D +E++ KIR +PY++V G +E E+G + V Sbjct: 535 DSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAV 594 Query: 580 RRREDGKQYETRIEELIKEIKEK 602 R R + E+I++++++ Sbjct: 595 RTRRGKDLGSMDVNEVIEKLQQE 617 >pdb|1EVK|A Chain A, Crystal Structure Of A Truncated Form Of Threonyl-Trna Synthetase With The Ligand Threonine >gi|9955216|pdb|1EVK|B Chain B, Crystal Structure Of A Truncated Form Of Threonyl-Trna Synthetase With The Ligand Threonine >gi|9955217|pdb|1EVL|A Chain A, Crystal Structure Of A Truncated Form Of Threonyl-Trna Synthetase With A Threonyl Adenylate Analog >gi|9955218|pdb|1EVL|B Chain B, Crystal Structure Of A Truncated Form Of Threonyl-Trna Synthetase With A Threonyl Adenylate Analog >gi|9955219|pdb|1EVL|C Chain C, Crystal Structure Of A Truncated Form Of Threonyl-Trna Synthetase With A Threonyl Adenylate Analog >gi|9955220|pdb|1EVL|D Chain D, Crystal Structure Of A Truncated Form Of Threonyl-Trna Synthetase With A Threonyl Adenylate Analog Length = 401 Score = 146 bits (366), Expect = 5e-34 Identities = 104/383 (27%), Positives = 180/383 (46%), Gaps = 17/383 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++ G I LE +V K+ EY EV+ P M D + + + + Sbjct: 20 PGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTT 79 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S ++ Y ++ C G I + SYR+LPLRM E R E G L GL R+R F Sbjct: 80 SSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS-CHRNEPSGSLHGLMRVRGF 138 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H + EQ ++E +L ++ G +++ R +++ W + Sbjct: 139 TQDDAHIFCTE-EQIRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAE 197 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 + ++ P ++ + F+ K EF D LD+A TVQ+D R + Sbjct: 198 ADLAVALEENNIPFEYQLGEGAFYG--PKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSAS 255 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y E+ E K P+++H + G++ER + + E+ A FP WL+P+QV ++ ++ Sbjct: 256 YVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGF--------FPTWLAPVQVVIMNIT 307 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 + +Y + KL A IRV D +E++ KIR +PY++V G +E E+G + V Sbjct: 308 DSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAV 367 Query: 580 RRREDGKQYETRIEELIKEIKEK 602 R R + E+I++++++ Sbjct: 368 RTRRGKDLGSMDVNEVIEKLQQE 390 >sp|P00955|SYT_ECOLI THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7428463|pir||SYECTT threonine--tRNA ligase (EC 6.1.1.3) - Escherichia coli >gi|5107656|pdb|1QF6|A Chain A, Structure Of E. Coli Threonyl-Trna Synthetase Complexed With Its Cognate Trna >gi|1742811|dbj|BAA15498.1| (D90814) Threonine--tRNA ligase (EC 6.1.1.3) [Escherichia coli] >gi|1742818|dbj|BAA15504.1| (D90815) Threonine--tRNA ligase (EC 6.1.1.3) [Escherichia coli] >gi|1788013|gb|AAC74789.1| (AE000267) threonine tRNA synthetase [Escherichia coli K12] Length = 642 Score = 146 bits (366), Expect = 5e-34 Identities = 104/383 (27%), Positives = 180/383 (46%), Gaps = 17/383 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++ G I LE +V K+ EY EV+ P M D + + + + Sbjct: 261 PGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTT 320 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S ++ Y ++ C G I + SYR+LPLRM E R E G L GL R+R F Sbjct: 321 SSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS-CHRNEPSGSLHGLMRVRGF 379 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H + EQ ++E +L ++ G +++ R +++ W + Sbjct: 380 TQDDAHIFCTE-EQIRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAE 438 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 + ++ P ++ + F+ K EF D LD+A TVQ+D R + Sbjct: 439 ADLAVALEENNIPFEYQLGEGAFYG--PKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSAS 496 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y E+ E K P+++H + G++ER + + E+ A FP WL+P+QV ++ ++ Sbjct: 497 YVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGF--------FPTWLAPVQVVIMNIT 548 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 + +Y + KL A IRV D +E++ KIR +PY++V G +E E+G + V Sbjct: 549 DSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAV 608 Query: 580 RRREDGKQYETRIEELIKEIKEK 602 R R + E+I++++++ Sbjct: 609 RTRRGKDLGSMDVNEVIEKLQQE 631 >emb|CAA23560.1| (V00291) threonyl-tRNA synthetase (thrS; EC 6.1.1.3) [Escherichia coli] Length = 642 Score = 146 bits (366), Expect = 5e-34 Identities = 104/383 (27%), Positives = 180/383 (46%), Gaps = 17/383 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++ G I LE +V K+ EY EV+ P M D + + + + Sbjct: 261 PGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTT 320 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S ++ Y ++ C G I + SYR+LPLRM E R E G L GL R+R F Sbjct: 321 SSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS-CHRNEPSGSLHGLMRVRGF 379 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H + EQ ++E +L ++ G +++ R +++ W + Sbjct: 380 TQDDAHIFCTE-EQIRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAE 438 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 + ++ P ++ + F+ K EF D LD+A TVQ+D R + Sbjct: 439 ADLAVALEENNIPFEYQLGEGAFYG--PKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSAS 496 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y E+ E K P+++H + G++ER + + E+ A FP WL+P+QV ++ ++ Sbjct: 497 YVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGF--------FPTWLAPVQVVIMNIT 548 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 + +Y + KL A IRV D +E++ KIR +PY++V G +E E+G + V Sbjct: 549 DSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAV 608 Query: 580 RRREDGKQYETRIEELIKEIKEK 602 R R + E+I++++++ Sbjct: 609 RTRRGKDLGSMDVNEVIEKLQQE 631 >emb|CAB70933.1| (AL137778) threonine-tRNA synthetase [Streptomyces coelicolor A3(2)] Length = 658 Score = 143 bits (356), Expect = 7e-33 Identities = 113/395 (28%), Positives = 182/395 (45%), Gaps = 22/395 (5%) Query: 201 AEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETP 260 AEK H KL E +L G ++PKG +I+ ++E Y + E G V TP Sbjct: 255 AEKRD-HRKLGSELDLFSIPEQIGSGLAVFHPKGGIIRRVMEDYSRRRHEEEGYEFVYTP 313 Query: 261 IMYDFEHPALEKYLNRFPARQYIVLSGDKR--YFLRFAACFGQFMIKKDAIISYRNLPLR 318 + +L+ + Y + D+ Y+L+ C +I SYR LPLR Sbjct: 314 HATKGKLFETSGHLDWYADGMYPPMQLDEGVDYYLKPMNCPMHNLIFDARGRSYRELPLR 373 Query: 319 MYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVG 378 ++E +R EK G + GL R R FT D H EQ EE K + +L G Sbjct: 374 LFEFGTV-YRYEKSGVVHGLTRARGFTQDDAHIYCTR-EQMSEELDKTLTFVLNLLRDYG 431 Query: 379 LTPEDYEVAIRFTEDF------WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFN 432 L E++ + E F W+E + + ++ + ++ + F+ K Sbjct: 432 LNDFYLELSTKDPEKFVGSDEAWEEATETLRQVAEKQNLELVADPGGAAFYG--PKISVQ 489 Query: 433 FVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQ 492 D + + +ST+Q+D ERF + Y +G + P+++H + G+IER +LE Sbjct: 490 ARDAIGRTWQMSTIQLDFNLPERFSLEYTAADGAKTRPVMIHRALFGSIERFFAVLLEHY 549 Query: 493 AKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKK 552 A M P WL+P+Q IPV + ++ Y A + +RVDVD +R+ KK Sbjct: 550 AGAM--------PPWLAPVQAVGIPVGDAHVQYLEEFAAEARRKGLRVDVDASSDRMQKK 601 Query: 553 IRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQ 587 IR +K+ +P++++VG + GT++ R R DG Q Sbjct: 602 IRTQQKQKVPFMIIVGDEDMHGGTVSFRYR-DGSQ 635 >sp|P52709|SYTC_CAEEL PROBABLE THREONYL-TRNA SYNTHETASE, CYTOPLASMIC (THREONINE--TRNA LIGASE) (THRRS) >gi|7497557|pir||T19994 hypothetical protein C47D12.6 - Caenorhabditis elegans >gi|3875006|emb|CAA93762.1| (Z69902) predicted using Genefinder~Similarity to Human threonyl-tRNA synthetase (SW:SYTC_HUMAN), contains similarity to Pfam domain: PF00587 (tRNA synthetase class II (G, H, P, S and T)), Score=493.9, E-value=4e-145, N=1~cDNA EST EMBL:M89192 comes fro> Length = 725 Score = 142 bits (354), Expect = 1e-32 Identities = 108/404 (26%), Positives = 193/404 (47%), Gaps = 27/404 (6%) Query: 207 HVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE 266 H KL EH+L + S PG+ +YPKG I + L ++ ++ G EV TP MY+ + Sbjct: 322 HRKLGKEHDLFFFHQLS-PGSAFWYPKGAHIYNKLVDFIRKQYRRRGFTEVITPNMYNKK 380 Query: 267 HPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYS 326 + + + + + + L+ C G ++ +Y LP R + Sbjct: 381 LWETSGHWQHYSEDMFKIEVEKEEFGLKPMNCPGHCLMFGHMPHTYNELPFRFADFG-VL 439 Query: 327 FRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGV-GLT----- 380 R E G L+GL R+R F D H + +Q EE K+ E V G T Sbjct: 440 HRNEMSGALTGLTRVRRFQQDDAHIFCRQ-DQISEEIKQCLDFLEYAYEKVFGFTFKLNL 498 Query: 381 ---PEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNL 437 PE + I + W + + + + G+ ++ F+ K + D L Sbjct: 499 STRPEGFLGNI----ETWDKAEADLTNALNASGRKWVLNPGDGAFYG--PKIDITIQDAL 552 Query: 438 DKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMN 497 + +T+Q+D + +F ++Y+DE GE++ P+++H + G++ER+ + E Sbjct: 553 KRNFQCATIQLDFQLPNQFDLSYFDEKGEKQRPVMIHRAVLGSVERMTAILTESYG---- 608 Query: 498 EGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDE-DERLNKKIRRA 556 GK +P WLSP Q ++I V E DYA V ++ A ++ ++ + +NK++R+A Sbjct: 609 -GK---WPFWLSPRQCKIITVHESVRDYANDVKKQIFEAGFEIEYEENCGDTMNKQVRKA 664 Query: 557 EKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK 600 + +I+V+GA+EKENGT+ VR R++ + E +++LI + + Sbjct: 665 QLAQFNFILVIGAKEKENGTVNVRTRDNAVRGEVALDKLISKFR 708 >sp|O06200|SYT_MYCTU THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437528|pir||E70571 probable thrS protein - Mycobacterium tuberculosis (strain H37RV) >gi|2104299|emb|CAB08628.1| (Z95387) thrS [Mycobacterium tuberculosis] Length = 692 Score = 139 bits (348), Expect = 6e-32 Identities = 107/393 (27%), Positives = 182/393 (46%), Gaps = 31/393 (7%) Query: 207 HVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE 266 H KL +E +L + G ++PKG +++ LE Y K E G V +P + + Sbjct: 270 HRKLGVELDLFSFPDEIGSGLAVFHPKGGIVRRELEDYSRRKHTEAGYQFVNSPHITKAQ 329 Query: 267 HPALEKYLNRFPARQYIVLSGD-------------KRYFLRFAACFGQFMIKKDAIISYR 313 +L+ + + + D + Y+L+ C +I + SYR Sbjct: 330 LFHTSGHLDWYADGMFPPMHIDAEYNADGSLRKPGQDYYLKPMNCPMHCLIFRARGRSYR 389 Query: 314 NLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEV 373 LPLR++E +R EK G + GL R+R TM D H +Q ++E + + +++ Sbjct: 390 ELPLRLFEFGTV-YRYEKSGVVHGLTRVRGLTMDDAHIFCTR-DQMRDELRSLLRFVLDL 447 Query: 374 LEGVGLTPEDYEVAIRFTEDF------WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFIL 427 L GLT E++ + E F W+E + E+ G ++ + F+ Sbjct: 448 LADYGLTDFYLELSTKDPEKFVGAEEVWEEATTVLAEVGAESGLELVPDPGGAAFYG--P 505 Query: 428 KFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYA 487 K D L + +ST+Q+D ERFG+ Y +G P+++H + G+IER Sbjct: 506 KISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTAADGTRHRPVMIHRALFGSIERFFGI 565 Query: 488 ILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDE 547 + E A FP WL+P+QV IPV++E++ Y VA +L+ +R +VD D+ Sbjct: 566 LTEHYA--------GAFPAWLAPVQVVGIPVADEHVAYLEEVATQLKSHGVRAEVDASDD 617 Query: 548 RLNKKIRRAEKEWIPYIVVVGAREKENGTITVR 580 R+ KKI +P++V+ G R+ G ++ R Sbjct: 618 RMAKKIVHHTNHKVPFMVLAGDRDVAAGAVSFR 650 >sp|P57225|SYT_BUCAI THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|10038808|dbj|BAB12843.1| (AP001118) threonyl-tRNA synthetase [Buchnera sp. APS] Length = 642 Score = 139 bits (348), Expect = 6e-32 Identities = 103/383 (26%), Positives = 180/383 (46%), Gaps = 17/383 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++ KG +I + L+ +V K+ EY EV+TP++ D + + + + Sbjct: 261 PGMIFWHNKGWIIFNELQNFVRVKLKEYKYEEVKTPLLIDKLIWKQSGHWDNYKNAIFTT 320 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 LS + Y ++ C G I + SYR+LP+RM E R E G L GL R+R F Sbjct: 321 LSEHREYCIKPMNCPGHVQIFNSRLKSYRDLPIRMAEFGS-CHRNEPSGSLHGLMRVRNF 379 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H EQ + E K+ ++ +++ R T+ W E + Sbjct: 380 TQDDAHIFCTR-EQVRSEINDCIKMIYDLYSTFHFKKILVKLSTRPEKRIGTDSLWNESE 438 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 + ++ LI + E ++ K EF D+LD+ T+Q+D R Sbjct: 439 KDLSDM--LIENHLSFEYQSGEGAFYGPKIEFILQDSLDRNWQCGTIQLDFYLPLRLSSF 496 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y +E E+K P+I+H + G+IER + ++E+ + + P WLSP+QV +I ++ Sbjct: 497 YINEKNEKKVPVIIHRAILGSIERFIGILIEECSGNL--------PTWLSPVQVVIISIT 548 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 + Y + K IR++ D +E++ KIR IPYI++ G +E + I+V Sbjct: 549 DISSGYVKELFKKFSDVNIRIECDLRNEKIGFKIREHTLRRIPYILICGEKESSSKKISV 608 Query: 580 RRREDGKQYETRIEELIKEIKEK 602 R R+ ++ IK+++++ Sbjct: 609 RNRQGHNFGMIDVDFFIKKLQKE 631 >sp|O04630|SYTC_ARATH PROBABLE THREONYL-TRNA SYNTHETASE, CYTOPLASMIC (THREONINE--TRNA LIGASE) (THRRS) >gi|7437517|pir||T01763 threonine--tRNA ligase (EC 6.1.1.3) - Arabidopsis thaliana >gi|2191162|gb|AAB61048.1| (AF007270) Similar to threonyl-tRNA synthetase; coded for by A. thaliana cDNA R65376 [Arabidopsis thaliana] Length = 702 Score = 139 bits (346), Expect = 1e-31 Identities = 124/467 (26%), Positives = 210/467 (44%), Gaps = 44/467 (9%) Query: 162 SYWYILTPEGELIEVDKFDFTGYENLRKFVNY-EIAKNRIAEKEPPHVKLMLEHELVDYE 220 +YW L V + + L+K++ + E AK +L H+L Sbjct: 257 AYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLLGQKQELFFSHQL---- 312 Query: 221 PGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPAR 280 PG+ + P G + + L ++ + G EV TP MY+ E + + + Sbjct: 313 ---SPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQTSGHADNYKDN 369 Query: 281 QYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRR 340 + + + L+ C G +I + + SYR LP+R+ + R E G LSGL R Sbjct: 370 MFTFNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPMRLADFG-VLHRNEASGALSGLTR 428 Query: 341 LRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAI-----RFTEDF- 394 +R F D H EQ K E + + V + G T YE+ + ++ D Sbjct: 429 VRRFQQDDAHIFCTT-EQVKGEVQGVLEFIDYVYKVFGFT---YELKLSTRPEKYLGDLE 484 Query: 395 -WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENA 453 W + + + E ++ GKP+++ F+ K + D +++ +T+Q+D + Sbjct: 485 TWDKAEADLKEAIEAFGKPLVLNEGDGAFYG--PKIDITVSDAMNRKFQCATLQLDFQLP 542 Query: 454 ERFGITYYDENGEEKY-PLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQ 512 RF + Y E+ +K P+++H + G++ER M+AIL + K GK +P W+SP Q Sbjct: 543 IRFNLEYAAEDEAKKSRPVMIHRAVLGSVER-MFAILLEHYK----GK---WPFWISPRQ 594 Query: 513 VRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKK-------------IRRAEKE 559 V P+SE+ YA V +++ A VD D D +++KK +R A+ Sbjct: 595 AIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVNDFGSCSKFCSVVREAQLA 654 Query: 560 WIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKEKTEGF 606 YI+VVG E G ++VR R++ IE+L++E K KT F Sbjct: 655 QYNYILVVGETEAATGQVSVRVRDNAAHSVKSIEDLLEEFKAKTAEF 701 >sp|Q9Z7A0|SYT_CHLPN THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|7437526|pir||G72034 threonine--tRNA ligase (EC 6.1.1.3) CP1065 [similarity] - Chlamydophila pneumoniae (strains CWL029 and AR39) >gi|4377117|gb|AAD18944.1| (AE001661) Threonyl tRNA Synthetase [Chlamydophila pneumoniae CWL029] >gi|7189978|gb|AAF38837.1| (AE002263) threonyl-tRNA synthetase [Chlamydophila pneumoniae AR39] >gi|8979180|dbj|BAA99014.1| (AP002548) threonyl tRNA synthetase [Chlamydophila pneumoniae J138] Length = 635 Score = 138 bits (343), Expect = 2e-31 Identities = 102/386 (26%), Positives = 181/386 (46%), Gaps = 19/386 (4%) Query: 223 SDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQY 282 S PG ++P+G ++ L +Y + G E+ TP + + + + + + + A Y Sbjct: 253 SSPGMPFFHPRGMIVWDALIRYWKQLHTAAGYKEILTPQLMNRQLWEVSGHWDNYKANMY 312 Query: 283 IVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLR 342 + D+ Y ++ C G + K + SY+ PLR+ E+ + R+E G LSGL R+R Sbjct: 313 TLQIDDEDYAIKPMNCPGCMLYYKTRLHSYKEFPLRVAEVG-HVHRQEASGALSGLMRVR 371 Query: 343 AFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTED------FWK 396 AF D H EQ +EE +L + GL E++ R +D W+ Sbjct: 372 AFHQDDAHVFLTP-EQVEEETLNILQLVSTLYGTFGLEYH-LELSTRPEKDTIGDDSLWE 429 Query: 397 EHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERF 456 D + + G P ++ + F+ K + + D + + T+Q+D+ ERF Sbjct: 430 LATDALNRALVQSGTPFIVRPGEGAFYG--PKIDIHVKDAIQRTWQCGTIQLDMFLPERF 487 Query: 457 GITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVI 516 + Y G + P++LH + G+IER + ++E K FPLWLSP QVR+I Sbjct: 488 ELEYTTAQGTKSVPVMLHRALFGSIERFLGILIENF--------KGRFPLWLSPEQVRII 539 Query: 517 PVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGT 576 V++ ++ A + + + V +DD E ++KKIR A+ + Y++ +G E Sbjct: 540 TVADRHIPRAKELEEAWKRLGLVVTLDDSSESVSKKIRNAQNMQVNYMITLGDHEINENV 599 Query: 577 ITVRRREDGKQYETRIEELIKEIKEK 602 + VR R++ + +E + I E+ Sbjct: 600 LAVRTRDNRVINDVSVERFLNTILEE 625 >sp|O84585|SYT_CHLTR THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|6578108|gb|AAC68183.2| (AE001328) Threonyl tRNA Synthetase [Chlamydia trachomatis] Length = 635 Score = 138 bits (343), Expect = 2e-31 Identities = 102/389 (26%), Positives = 177/389 (45%), Gaps = 23/389 (5%) Query: 230 YYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDK 289 ++P+G ++ + L Y G +++TP + + E + + + Y + ++ Sbjct: 260 FHPRGMVVWNALVDYWKRLHQRAGYQQIQTPQLMNRELWEISGHWENYKENMYTLTVDEE 319 Query: 290 RYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDM 349 Y ++ C G + K + SYR PLR+ E+ + R E G LSGL R+R F D Sbjct: 320 DYAIKPMNCPGCMLYYKTQLHSYREFPLRIAEIG-HVHRHELSGALSGLMRVRTFHQDDA 378 Query: 350 HTLAKDIEQAKEEFKKQFKLSMEVLEGVGLT--------PEDYEVAIRFTEDFWKEHKDF 401 H EQ +EE L E+ GL PE + ++D W+ Sbjct: 379 HVFLTP-EQVEEETLNILNLVSELYGTFGLEYHLELSTRPEQGTIG---SDDLWELATKA 434 Query: 402 IVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYY 461 + + KP +I + F+ K + + D +++ T+Q+D+ ERF + Y Sbjct: 435 LKRALVKSQKPFIISPGEGAFYG--PKIDIHVKDAINRTWQCGTIQLDMFLPERFDLKYT 492 Query: 462 DENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEE 521 + GE+ P++LH + G+IER + ++E K FPLWLSP VR+I V++ Sbjct: 493 NAQGEKSTPIMLHRALFGSIERFLGILIEHF--------KGRFPLWLSPEHVRIITVADR 544 Query: 522 YLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRR 581 + A +A I V VD +E ++KKIR A+ + Y++ +G +E E + VR Sbjct: 545 HEARAQELAKHFSQMGIIVSVDSSNESVSKKIRNAQNMQVNYMITIGDKELETHLLAVRT 604 Query: 582 REDGKQYETRIEELIKEIKEKTEGFPYKP 610 R++ + +E+ I E+ P Sbjct: 605 RDNRVLNDIAVEQFSHVILEELRSLSLTP 633 >pir||G71497 threonine--tRNA ligase (EC 6.1.1.3) - Chlamydia trachomatis (serotype D, strain UW3/Cx) Length = 660 Score = 138 bits (343), Expect = 2e-31 Identities = 102/389 (26%), Positives = 177/389 (45%), Gaps = 23/389 (5%) Query: 230 YYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDK 289 ++P+G ++ + L Y G +++TP + + E + + + Y + ++ Sbjct: 285 FHPRGMVVWNALVDYWKRLHQRAGYQQIQTPQLMNRELWEISGHWENYKENMYTLTVDEE 344 Query: 290 RYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDM 349 Y ++ C G + K + SYR PLR+ E+ + R E G LSGL R+R F D Sbjct: 345 DYAIKPMNCPGCMLYYKTQLHSYREFPLRIAEIG-HVHRHELSGALSGLMRVRTFHQDDA 403 Query: 350 HTLAKDIEQAKEEFKKQFKLSMEVLEGVGLT--------PEDYEVAIRFTEDFWKEHKDF 401 H EQ +EE L E+ GL PE + ++D W+ Sbjct: 404 HVFLTP-EQVEEETLNILNLVSELYGTFGLEYHLELSTRPEQGTIG---SDDLWELATKA 459 Query: 402 IVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYY 461 + + KP +I + F+ K + + D +++ T+Q+D+ ERF + Y Sbjct: 460 LKRALVKSQKPFIISPGEGAFYG--PKIDIHVKDAINRTWQCGTIQLDMFLPERFDLKYT 517 Query: 462 DENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEE 521 + GE+ P++LH + G+IER + ++E K FPLWLSP VR+I V++ Sbjct: 518 NAQGEKSTPIMLHRALFGSIERFLGILIEHF--------KGRFPLWLSPEHVRIITVADR 569 Query: 522 YLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRR 581 + A +A I V VD +E ++KKIR A+ + Y++ +G +E E + VR Sbjct: 570 HEARAQELAKHFSQMGIIVSVDSSNESVSKKIRNAQNMQVNYMITIGDKELETHLLAVRT 629 Query: 582 REDGKQYETRIEELIKEIKEKTEGFPYKP 610 R++ + +E+ I E+ P Sbjct: 630 RDNRVLNDIAVEQFSHVILEELRSLSLTP 658 >sp|P47615|SYT_MYCGE THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|1361863|pir||E64241 threonine--tRNA ligase (EC 6.1.1.3) - Mycoplasma genitalium >gi|3844963|gb|AAC71602.1| (U39719) threonyl-tRNA synthetase (thrS) [Mycoplasma genitalium] Length = 564 Score = 137 bits (342), Expect = 3e-31 Identities = 107/392 (27%), Positives = 185/392 (46%), Gaps = 23/392 (5%) Query: 194 EIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYG 253 ++ K + H L + EL ++P G + KG +++++ +V + + +G Sbjct: 155 QLIKENEERLKKDHRSLGKQLELFSFDPLIGAGLPIWLAKGTTLRNIIGNFVHHQQLLFG 214 Query: 254 AMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKR-YFLRFAACFGQFMIKKDAIISY 312 V +P++ + E + + + + + D + LR C +I K SY Sbjct: 215 FNTVCSPVLANIELFKISGHYQHYKEDMFPAIKLDSQAMMLRPMTCPHHCLIFKQKRYSY 274 Query: 313 RNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSME 372 + +P R E R E G L GL R+R T+ D H + +Q K E K F L + Sbjct: 275 KKMPQRFSE-DSILHRFEASGGLIGLERVRCMTLLDNHIFCR-ADQIKSEIKNAFNLIQK 332 Query: 373 V-------LEGVGLTPEDYEVAIRFTED--FWKEHKDFIVELVKLIGKPVLIEMWKQRFF 423 V + + L+ D + +F ++ W+E + + ++K + E+ F+ Sbjct: 333 VNKKFGFIFDRIDLSLHDPKNQSKFIDNPGLWRESESQMENVLKDLNIQYQKEIGAAAFY 392 Query: 424 YFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIER 483 K +F F K ++T+Q+D E+F +TY D+ K P+I+H G ER Sbjct: 393 G--PKIDFQFKTIFKKMITIATIQLDFLLPEKFDLTYIDKKNTLKKPVIIHVGIIGTYER 450 Query: 484 VMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS-EEYLDYALYVAGKLEGAKIRVDV 542 + A+LEK + FPLWL+P+Q +IPV+ +++L A + KL IRV++ Sbjct: 451 FIAALLEKTS--------GNFPLWLAPVQAVIIPVNIQKHLKAAKKLYNKLLKENIRVNL 502 Query: 543 DDEDERLNKKIRRAEKEWIPYIVVVGAREKEN 574 DD +RL KK+R+A E IP ++VG +E EN Sbjct: 503 DDNQDRLAKKVRQAIIEKIPLQLIVGDKEIEN 534 >sp|P75225|SYT_MYCPN THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|2146662|pir||S73615 threonine--tRNA ligase (EC 6.1.1.3) thrSv - Mycoplasma pneumoniae (strain ATCC 29342) >gi|1673960|gb|AAB95937.1| (AE000027) threonyl-tRNA synthetase [Mycoplasma pneumoniae] Length = 564 Score = 137 bits (342), Expect = 3e-31 Identities = 109/399 (27%), Positives = 188/399 (46%), Gaps = 24/399 (6%) Query: 215 ELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYL 274 E+ ++P G + KG ++K ++ +V + + +G V +P++ + E + + Sbjct: 176 EIFTFDPLVGAGFPIWLEKGAVLKKIIGDFVHYQQLMFGFKTVSSPVLANLELYKISGHY 235 Query: 275 NRFPARQY-IVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRG 333 + + V ++ LR C +I K SY+ LP R E R E G Sbjct: 236 VHYSEDMFPSVKLENQMMMLRPMTCPHHCLIFKHKRRSYKELPQRFCE-DSILHRFEASG 294 Query: 334 ELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRF--- 390 L+GL R+R T+ D H + +Q K E K FKL V G + + ++A+ Sbjct: 295 GLTGLERVRCMTLLDNHIFCRT-DQIKAEIKNAFKLIQTVNNKFGFSFDRIDLALHDPNN 353 Query: 391 ------TEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALS 444 + W + I + K + P I++ F+ K +F F L+K ++ Sbjct: 354 KAKFIDNDQLWANSESQIESVFKELNVPYKIDVGAAAFYG--PKIDFQFKTALNKMITIA 411 Query: 445 TVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMF 504 T+Q+D ERF + Y+DE + P+I+H G ER + A+LEK G Sbjct: 412 TIQLDFLLPERFELRYFDEQSNAQTPVIIHVGIVGTYERFIAALLEK-----THGN---L 463 Query: 505 PLWLSPIQVRVIPVS-EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPY 563 PLW++P+QV VIPV+ +++ A + KL IRV +D+ ++RL KK+R A IP Sbjct: 464 PLWMAPVQVVVIPVNIDKHEKAAKKLYQKLLKENIRVSLDESEDRLGKKVRTAIVNKIPL 523 Query: 564 IVVVGAREKENGT-ITVRRREDGKQYETRIEELIKEIKE 601 ++VG +E ++ T IT R + K + +K++++ Sbjct: 524 QIIVGDKEMQDLTRITCRGFKGEKVQKLYWANFVKKVRK 562 >pir||F83303 threonyl-tRNA synthetase PA2744 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9948822|gb|AAG06132.1|AE004702_9 (AE004702) threonyl-tRNA synthetase [Pseudomonas aeruginosa] Length = 640 Score = 136 bits (339), Expect = 7e-31 Identities = 103/382 (26%), Positives = 175/382 (44%), Gaps = 20/382 (5%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++P G + +LEQY+ + ++G +EV TP + D + + + + Sbjct: 260 PGMVFWHPNGWSVYQVLEQYMRKVQRDHGYVEVRTPQVVDRILWERSGHWSNYAENMFTT 319 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S + Y ++ C I + SYR+LPLR+ E R E G L G+ R+R F Sbjct: 320 SSESRDYAVKPMNCPCHVQIFNQGLKSYRDLPLRLAEFGA-CHRNEPSGALHGIMRVRGF 378 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H + EQ K+E KL+++V G T +++ R +++ W + Sbjct: 379 TQDDAHIFCTE-EQVKKEAADFIKLTLQVYRDFGFTDIAMKLSTRPAKRVGSDELWDRAE 437 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 + + + G + E ++ K EF D L + T+Q D ER + Sbjct: 438 GALADALNESG--LAWEYQPGEGAFYGPKIEFTLKDCLGRNWQCGTLQYDPNLPERLDAS 495 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y E+ K P++LH + G+ ER + ++E A FP WL+P Q V+ ++ Sbjct: 496 YIAEDNNRKRPVMLHRAILGSFERFIGMLIEHYAGA--------FPAWLAPTQAVVMNIT 547 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 ++ D+A V L + R D +E++ KIR +PY++V+G RE E+ + V Sbjct: 548 DKQADFAAEVVRILGESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDREVESKAVAV 607 Query: 580 RRRED---GKQYETRIEELIKE 598 R RE G T+ EL+ + Sbjct: 608 RTREGEDLGSMPVTQFAELLAQ 629 >pir||D82770 threonyl-tRNA synthetase XF0736 [imported] - Xylella fastidiosa (strain 9a5c) >gi|9105624|gb|AAF83546.1|AE003915_11 (AE003915) threonyl-tRNA synthetase [Xylella fastidiosa] Length = 635 Score = 135 bits (336), Expect = 2e-30 Identities = 98/365 (26%), Positives = 165/365 (44%), Gaps = 15/365 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++PKG +I +E Y+ G E+ P + D + + + + Sbjct: 262 PGLVFWHPKGWVIWQTIENYIRRVYRNSGYGELRCPQILDVSLWQKSGHWDNYKENMFFT 321 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S + Y ++ C G + + SYR+LP+R E R E G L GL R+R F Sbjct: 322 DSEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRYGEFGA-CHRNEPSGALHGLLRVRGF 380 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIVE 404 T D H + Q + E + +++V G ++A+R + E Sbjct: 381 TQDDGHIFCTEA-QIESEVTAFHRQALQVYADFGFEDIQIKIALRPDKRLGDSLSWDKAE 439 Query: 405 LVKLIGKPVLIEMWKQ---RFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYY 461 W++ ++ K E++ D + + L T+Q+D R G Y Sbjct: 440 AALRAALSACEVEWQELPGEGAFYGPKIEYHLKDAIGRTWQLGTIQVDFMMPGRLGAEYV 499 Query: 462 DENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEE 521 DE + +P++LH + G++ER + ++E A + +P WL+P+QV V +++ Sbjct: 500 DERSQRCHPVMLHRAIVGSMERFIGILIEHYAGI--------WPTWLAPVQVVVANITDA 551 Query: 522 YLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVR- 580 +YA V L RV+ D +E++ KIR + +PY++VVG REKENGT+ VR Sbjct: 552 QYEYAERVHKALLNQGFRVNTDLRNEKIGYKIREHTLQRVPYLLVVGDREKENGTVAVRT 611 Query: 581 -RRED 584 RED Sbjct: 612 CSRED 616 >pir||A81656 threonyl-tRNA synthetase TC0870 [imported] - Chlamydia muridarum (strain Nigg) >gi|7190897|gb|AAF39666.1| (AE002353) threonyl-tRNA synthetase [Chlamydia muridarum] Length = 635 Score = 134 bits (334), Expect = 3e-30 Identities = 101/381 (26%), Positives = 174/381 (45%), Gaps = 23/381 (6%) Query: 230 YYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDK 289 ++P+G +I + L Y G +++TP + E + + + Y + ++ Sbjct: 260 FHPRGMVIWNALIDYWKRLHQLAGYQQIQTPQLMSRELWEISGHWENYKENMYTLTVDEE 319 Query: 290 RYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDM 349 Y ++ C G + K + SYR PLR+ E+ + R E G LSGL R+R F D Sbjct: 320 DYAIKPMNCPGCMLYYKTQLHSYREFPLRIAEIG-HVHRHELSGALSGLMRVRTFHQDDA 378 Query: 350 HTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRF--------TEDFWKEHKDF 401 H EQ +EE L E+ GL DY + + ++D W+ + Sbjct: 379 HVFLTP-EQVEEETLNILNLVSELYGTFGL---DYHLELSTRPEQGTIGSDDLWELATEA 434 Query: 402 IVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYY 461 + + KP +I + F+ K + + D +++ T+Q+D+ ERF + Y Sbjct: 435 LKNALVKSQKPFIISPGEGAFYG--PKIDIHVKDAINRTWQCGTIQLDMFLPERFDLKYT 492 Query: 462 DENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEE 521 + GE+ P++LH + G+IER + ++E K FPLWLSP VR+I V++ Sbjct: 493 NPQGEKSTPIMLHRALFGSIERFLGILIEHF--------KGRFPLWLSPEHVRIITVADR 544 Query: 522 YLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRR 581 + A +A I VD E ++KKIR A+ + Y++ +G +E E + VR Sbjct: 545 HEARAQELAKYFTQQGILATVDQASESVSKKIRNAQNMQVNYMITIGDKELETQLLAVRT 604 Query: 582 REDGKQYETRIEELIKEIKEK 602 R++ + +E I E+ Sbjct: 605 RDNRVLNDISVEHFTNTILEE 625 >sp|P43014|SYT_HAEIN THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|1075350|pir||H64119 threonine--tRNA ligase (EC 6.1.1.3) - Haemophilus influenzae >gi|1574199|gb|AAC23014.1| (U32816) threonyl-tRNA synthetase (thrS) [Haemophilus influenzae Rd] Length = 643 Score = 133 bits (332), Expect = 5e-30 Identities = 103/383 (26%), Positives = 169/383 (43%), Gaps = 17/383 (4%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIV 284 PG + ++ G I LE +V K+ +Y EV+ P M D + + + Sbjct: 261 PGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTT 320 Query: 285 LSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAF 344 S ++ Y ++ C G I + SYR+LP+RM E R E G L GL R+R F Sbjct: 321 QSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGS-CHRNEPSGSLHGLMRVRGF 379 Query: 345 TMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIR-----FTEDFWKEHK 399 T D H + +Q + E K+ ++ G T +++ R +++ W + Sbjct: 380 TQDDAHIFCTE-DQIESEVTSCIKMVYDIYSTFGFTNIAVKLSTRPENRIGSDEMWDRAE 438 Query: 400 DFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGIT 459 + + G I+ + F+ K EF D L + TVQ+D R T Sbjct: 439 AGLAAALAHNGLEYEIQEGEGAFYG--PKIEFALRDCLGREWQCGTVQLDFALPGRLDAT 496 Query: 460 YYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVS 519 Y E+ K P+++H + G+IER + I E+ A FP WL+P Q V+ ++ Sbjct: 497 YVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGF--------FPAWLAPTQAVVMNIT 548 Query: 520 EEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITV 579 + DY VA +L +RV D +E++ KIR +PY++V G +E G + V Sbjct: 549 DSQADYVQKVAKQLSDVGLRVKTDLRNEKVGFKIREHTLRRVPYMLVCGDKEIAEGKVAV 608 Query: 580 RRREDGKQYETRIEELIKEIKEK 602 R R+ +EE + +K + Sbjct: 609 RTRKGADLGTFTVEEFAEILKNQ 631 >gi|4507367 threonyl-tRNA synthetase [Homo sapiens] >gi|135177|sp|P26639|SYTC_HUMAN THREONYL-TRNA SYNTHETASE, CYTOPLASMIC (THREONINE--TRNA LIGASE) (THRRS) >gi|319870|pir||YSHUT threonine--tRNA ligase (EC 6.1.1.3) - human >gi|1464742|gb|AAB04939.1| (M63180) threonyl-tRNA synthetase [Homo sapiens] Length = 712 Score = 133 bits (331), Expect = 6e-30 Identities = 122/457 (26%), Positives = 202/457 (43%), Gaps = 39/457 (8%) Query: 162 SYWYILTPEGELIEVDKFDFTGYENLRKFVNY-EIAKNRIAEKEPPHVKLMLEHELVDYE 220 +YW L + F + L+++ + E AKNR K +L HEL Sbjct: 271 TYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHEL---- 326 Query: 221 PGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPAR 280 PG+ + PKG I + L +++ + + G EV TP +++ + + Sbjct: 327 ---SPGSCFFLPKGVYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSEN 383 Query: 281 QYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRR 340 + + + L+ C G ++ S+R LPLR+ + R E G L+GL R Sbjct: 384 MFSFEVEKELFALKPMNCPGHSLMFDHRPRSWRELPLRLADFGGLH-RNELSGALTGLTR 442 Query: 341 LRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLT--------PEDYEVAIRFTE 392 +R F D H +EQ ++E K V G + PE + I + Sbjct: 443 VRRFQQDDAHIFCA-MEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWD 501 Query: 393 DFWKEHKDFIVELVKLIGKPVLIEMWKQRF---FYFILKFEFNFVDNLDKAAALSTVQID 449 K+ ++ + E E W+ ++ K + D + + +T+Q+D Sbjct: 502 QAEKQLENSLNEFG---------EKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLD 552 Query: 450 VENAERFGITYYDENGEEKY-PLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWL 508 + RF +TY +GE+K P+I+H + G++ER++ + E N G K + P WL Sbjct: 553 FQLPIRFNLTYVSHDGEDKKRPVIVHRAILGSVERMIAILTE------NYGGK-LAPFWL 605 Query: 509 SPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVD-DEDERLNKKIRRAEKEWIPYIVVV 567 SP QV V+PV +YA V + AK D+D D LNKKIR A+ +I+VV Sbjct: 606 SPRQVMVVPVGPTCDEYAQNVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVV 665 Query: 568 GAREKENGTITVRRREDGKQYETRIEELIKEIKEKTE 604 G +EK GT+ +R R++ E I E I+ +++ E Sbjct: 666 GEKEKITGTVNIRTRDNKVHGERTISETIERLQQLKE 702 >sp|P87144|SYTC_SCHPO THREONYL-TRNA SYNTHETASE, CYTOPLASMIC (THREONINE--TRNA LIGASE) (THRRS) >gi|7493479|pir||T39997 Ths1p - fission yeast (Schizosaccharomyces pombe) >gi|2104451|emb|CAB08788.1| (Z95397) threonyl-trna synthetase, cytoplasmic [Schizosaccharomyces pombe] Length = 703 Score = 129 bits (321), Expect = 9e-29 Identities = 117/419 (27%), Positives = 188/419 (43%), Gaps = 35/419 (8%) Query: 207 HVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE 266 H K+ + EL + S PG+ + P G I + L +Y+ + + G EV TP MY+ Sbjct: 291 HRKIGRDQELFFFNEIS-PGSCFFLPHGARIYNTLLKYMRYQYSKRGYQEVITPNMYNVN 349 Query: 267 HPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYS 326 + N + + ++Y L+ C G ++ K SYR+LP R+ + Sbjct: 350 LWKTSGHWNNYSENMFSFDIEKEKYALKPMNCPGHCVMFKSRDRSYRDLPWRVADFG-VL 408 Query: 327 FRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEV 386 R E G LSGL R+R F D H +Q + E + F EV G T E+ Sbjct: 409 HRNEFSGALSGLTRVRRFQQDDAHIFCTP-DQVRSEIEGCFDFLKEVYGTFGFTFH-LEL 466 Query: 387 AIRFTEDF------WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKA 440 + R E + W + + + + G + F+ K + D L + Sbjct: 467 STRPEEKYLGDLATWDKAEAQLKAALDASGYKWELNAGDGAFYG--PKIDITVFDALKRQ 524 Query: 441 AALSTVQIDVENAERFGITYY---------DENGEEKY--PLILHCSPSGAIERVMYAIL 489 +T+Q+D + ERF + ++ + KY P+++H + G++ER++ + Sbjct: 525 HQCATIQLDFQLPERFQLEFHAPASAEEAKESGNNNKYTRPVMVHRAILGSLERMIAILT 584 Query: 490 EKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERL 549 E A GK +P W+SP QV +IPVS +YA V L A I VD D D L Sbjct: 585 EHYA-----GK---WPFWMSPRQVCIIPVSAAAYNYADKVHNILSEADIFVDTDKSDNTL 636 Query: 550 NKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYE----TRIEELIKEIKEKTE 604 KKIR A+ +I VVGA E+ ++ VR R+D K+ +EE++K++ + E Sbjct: 637 PKKIRNAQLAQYNFIFVVGAEEESTNSVNVRNRDDPKKQSKGSTVAVEEVVKKLLKLKE 695 >emb|CAC11475.1| (AL445064) threonine--tRNA ligase related protein [Thermoplasma acidophilum] Length = 660 Score = 129 bits (320), Expect = 1e-28 Identities = 122/455 (26%), Positives = 198/455 (42%), Gaps = 61/455 (13%) Query: 192 NYEIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIE 251 N E AK R H +++ E +L + PG Y G+ I+ L Y+ + Sbjct: 217 NLEEAKRR------DHRRIIAEMDLAVFNSEWAPGFPLYTQYGQTIRKELLSYMDSMNRK 270 Query: 252 YGAMEVETPIMY-DFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAII 310 G +V TP ++ D Y P L Y ++ C G I Sbjct: 271 NGWFDVWTPHVFRDTIWKQSGHYAKYRPNMYLFTLPDGDSYGIKPMNCPGHIAIFSRRKY 330 Query: 311 SYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLS 370 SYR+LP+R Y +R EK GE+ GL R RAFT D H + ++Q +E + + Sbjct: 331 SYRDLPVR-YSEPGTVYRYEKSGEVGGLTRPRAFTQDDGHAFLR-MDQIGDEIRTLLTMV 388 Query: 371 MEVLE----GVGLT----------PEDYEVAI------------RFTE------------ 392 +V + L+ PE+Y V+ R T+ Sbjct: 389 KDVFTILFGNIELSFDLSVIDRDHPENYLVSYVCRNCGYRIEGARGTDIECPVCHSHDLE 448 Query: 393 ---DFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQID 449 W + + + +K +G + + + ++ K + + D + + LST+Q+D Sbjct: 449 PDLSVWDNATEQLRDALKSMG--IEYKEYPGEAAFYGPKIDVHVKDAIGRMWQLSTIQLD 506 Query: 450 VENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLS 509 FG+TY + G+E +++H + G+ ERVM +LE A GK P WL+ Sbjct: 507 FFMPVNFGLTYTNSEGKEDRVVMIHRAIYGSYERVMAILLEHYA-----GK---LPTWLT 558 Query: 510 PIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGA 569 PIQ V+P+S + +YA +V +L IR ++D E ++KKI+ YI+VVG+ Sbjct: 559 PIQTYVVPISSNFDEYARHVHEELIRHGIRSEIDTSQETVSKKIKLIHPYRPAYIIVVGS 618 Query: 570 REKENGTITVRRREDGKQYETRIEELIKEIKEKTE 604 +E E ++T R RE GK + E I +K++ E Sbjct: 619 KEMETNSVTARNRE-GKSKTYSLSEFIDVVKKEIE 652 >emb|CAC10316.1| (AL442629) SCH63.25, possible threonyl tRNA synthetase, len: 430 aa; highly similar to C-terminal region of many threonyl tRNA synthetases, e.g. SW:SYT1_BACSU (EMBL:M36594) Bacillus subtilis threonyl tRNA synthetase 1 (EC 6.1.1.3) ThrS, 643 aa; fasta s> Length = 430 Score = 128 bits (318), Expect = 2e-28 Identities = 109/416 (26%), Positives = 187/416 (44%), Gaps = 26/416 (6%) Query: 199 RIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVE 258 R +++ H +L E L D +P G + P G +++ LE+YV E G V Sbjct: 21 RSHDRDHDHRRLGRELALFDTDPLMGAGLPYWLPDGAVVRHTLEEYVREAERRAGYRHVY 80 Query: 259 TPIMYDFEHPALEKYLNRFPARQYIVLS-GDKRYFLRFAACFGQFMIKKDAIISYRNLPL 317 +P++ E + + + + + G + LR + C ++ + SYR LPL Sbjct: 81 SPVLGKRELYEISGHWEHYRDDMFPPMELGAEEVVLRPSLCPHHALVYRSRARSYRELPL 140 Query: 318 RMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGV 377 R+ EL +R E G L GL R+RA + D H +QA E + L + Sbjct: 141 RIAELGAM-YRSEPSGVLGGLSRVRAIHLNDAHIFCTP-DQAAAEARAALGLIRRAHADL 198 Query: 378 GLTPEDYEVAI-----RFTED--FWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFE 430 G+ + +++ ++ D W+ + E L G P E + ++ K + Sbjct: 199 GIRAARHRLSLPGPGGKYVADPELWRRATALLEEA--LDGLPY--EAVEGEAAFYGPKID 254 Query: 431 FNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILE 490 D + + LSTVQID ERFG+ Y +G P+++H S G++ER + ++E Sbjct: 255 VQIEDAAGRESTLSTVQIDFHQPERFGLRYLGPDGARHRPVMVHRSVIGSVERAVAHLIE 314 Query: 491 KQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDV-DDEDERL 549 FP WL+P+Q+ V+PV+ + A + + +R +V D E L Sbjct: 315 AHG--------GAFPAWLAPVQLVVLPVAGAQREAAYALVRQATDLGLRAEVADPERGSL 366 Query: 550 NKKIRRAEKEWIPYIVVVGAREKENG-TITVRRREDGKQYETRIEELIKEIKEKTE 604 ++R A K +PY V+G RE ++G + VR R+ + + EL+ I ++ E Sbjct: 367 GARVRAARK--VPYQAVIGVREAQDGDLVDVRLRDGRRPGQLSGTELLHRITDRVE 420 >pir||E81439 threonine--tRNA ligase (EC 6.1.1.3) Cj0206 [imported] - Campylobacter jejuni (strain NCTC 11168) >gi|6967699|emb|CAB72689.1| (AL139074) threonyl-tRNA synthetase [Campylobacter jejuni] Length = 602 Score = 126 bits (313), Expect = 8e-28 Identities = 111/430 (25%), Positives = 191/430 (43%), Gaps = 29/430 (6%) Query: 181 FTGYENLRKFVNY-EIAKNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKS 239 F E+L++++ E AK R H KL E +L ++ G + G ++S Sbjct: 188 FADKESLKEYLTIIEEAKKR------DHRKLGTELKLFTFDDEIGGGLPIWLSNGARLRS 241 Query: 240 LLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACF 299 LE + + G V P + + + + + Y ++ Y ++ C Sbjct: 242 KLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNCV 301 Query: 300 GQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQA 359 G I + + SYR+LPL+ +E R EK G L GL R+R FT D H Q Sbjct: 302 GHIKIYQSDVRSYRDLPLKFFEYG-VVHRHEKSGVLHGLFRVREFTQDDAHIFCMP-SQI 359 Query: 360 KEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFT-------EDFWKEHKDFIVELVKLIGKP 412 KE+ + +++ L YE+ I ++ W+ + E + G Sbjct: 360 KEQVLEILAFVDNLMK---LFDFSYEMEISTKPEKAIGDDEIWEIATKALKEALDEQGLK 416 Query: 413 VLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLI 472 I+ F+ K + D L + T+Q+D RF + Y D + E+K P++ Sbjct: 417 YGIDEGGGAFYG--PKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVM 474 Query: 473 LHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGK 532 LH + G+ ER + + E A FP +++P V ++P+ E ++ YA + + Sbjct: 475 LHRAILGSFERFIGILTEHCAG--------EFPFFIAPTAVGIVPIGEAHIAYAKEIQKE 526 Query: 533 LEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRI 592 L I +V +++E L+KKIR AEK+ +P I+V+G E ++ +R R +Q + Sbjct: 527 LLELNIDSEVYEKNESLSKKIRIAEKQKLPMILVLGDDEVAKRSVALRDRRAKEQKNLSL 586 Query: 593 EELIKEIKEK 602 +E IK +KEK Sbjct: 587 DEFIKLVKEK 596 >gb|AAK40712.1| Threonyl-tRNA synthetase homolog (thrS-like) [Sulfolobus solfataricus] Length = 386 Score = 118 bits (294), Expect = 1e-25 Identities = 70/158 (44%), Positives = 96/158 (60%), Gaps = 14/158 (8%) Query: 1 MRVLLIHSDYIEYEVKDKALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKA 60 M +L IH+ + VK++A+K PE +K ++ LV F +VEK D+ EE+ KA Sbjct: 1 MIILFIHASDFSFNVKERAIKEPEEAK--LKSIELKNTLVCFTTVEKGDD---EEILSKA 55 Query: 61 IEEISKVAEQVKAENVFVYPFAHLSSELAKPSVAMDILNRVYQGLKERGFNVGKAPFGYY 120 I++I V +VKA++V +YP+AHLSS LA P A+ IL + LK++ V +APFG+Y Sbjct: 56 IDDILDVYSKVKADSVVIYPYAHLSSNLANPDTAIKILESLENLLKDK-VKVYRAPFGWY 114 Query: 121 KAFKISCKGHPLAELSRTIVPEEARVEEVPEALRKEEE 158 KAF ISC GHPL+ELSR I E L K EE Sbjct: 115 KAFSISCYGHPLSELSRRI--------RKTEELEKSEE 144 Score = 108 bits (267), Expect = 2e-22 Identities = 51/121 (42%), Positives = 81/121 (66%), Gaps = 5/121 (4%) Query: 484 VMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVD 543 + Y ILE + + P P WL+PIQVR++PV +++LD+++ VA +L IRV++D Sbjct: 254 IYYFILESVKQ-----QPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRKEGIRVNID 308 Query: 544 DEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKEKT 603 D D+ L KIRRA +WIP+++V+G RE + T+TV+ R +Q +EEL+KEIK++ Sbjct: 309 DLDDSLGNKIRRAGTDWIPFVIVIGEREVKTNTLTVKIRARNEQKSMTVEELVKEIKDEV 368 Query: 604 E 604 + Sbjct: 369 K 369 >sp|P46244|SYT_BUCAP THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|2118340|pir||I40071 threonine--tRNA ligase (EC 6.1.1.3) - Buchnera aphidicola >gi|868027|gb|AAC43605.1| (U11066) threonyl-tRNA synthetase [Buchnera aphidicola] >gi|1098080|prf||2115235B Thr-tRNA synthetase [Buchnera aphidicola] Length = 642 Score = 116 bits (288), Expect = 7e-25 Identities = 100/391 (25%), Positives = 179/391 (45%), Gaps = 32/391 (8%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEK---VIEYGAMEVETPIMYDFEHPALEKYLNRFPARQ 281 PG + ++ G +I + LE +V EK +I Y EV+TP++ + + + R Sbjct: 260 PGMIFWHNNGWIIFNELEIFVREKLKGIINY--KEVKTPLLIHKSIWQKSGHWDNYQDRI 317 Query: 282 YIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRL 341 + S ++ Y ++ C G I + SYR+LP+RM E R E G L GL R+ Sbjct: 318 FTTSSENREYCIKPMNCPGHVQIFNCGLKSYRDLPIRMAEFGS-CHRNESSGSLHGLMRI 376 Query: 342 RAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFT---------E 392 R FT D H EQ + E K+ ++ ++ ++F+ E Sbjct: 377 RNFTQDDAHIFCTQ-EQLRYEINNCIKMIYDLYSTFNFK----KILVKFSTRPKKRIGDE 431 Query: 393 DFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVEN 452 W + + + ++ LI + E + ++ K EF D+LD+ T+Q+D Sbjct: 432 SVWDQAEKDLSDV--LIENNLKFEHQEGEGAFYGPKIEFVLQDSLDRNWQCGTIQLDFYL 489 Query: 453 AERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQ 512 R Y DE+ +K P+I+H + G+IER + ++E+ ++ P WLSPIQ Sbjct: 490 PIRLRSFYIDEHNHQKIPIIIHRAILGSIERFIGILIEEFQEI---------PTWLSPIQ 540 Query: 513 VRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREK 572 V ++ +++ +++Y + IRV+ D +E++ KIR + + +EK Sbjct: 541 VVILSITDSHINYVKKIVQHFSDINIRVESDLRNEKIGFKIRATYIASNSIYINLWRKEK 600 Query: 573 EN-GTITVRRREDGKQYETRIEELIKEIKEK 602 N I+VR R I+ IK+++++ Sbjct: 601 LNLKKISVRTRNGYNLGIIDIDCFIKKLQKE 631 >emb|CAA21975.1| (AL033497) mitochondrial threonyl tRNA synthetase [Candida albicans] Length = 455 Score = 116 bits (288), Expect = 7e-25 Identities = 98/434 (22%), Positives = 190/434 (43%), Gaps = 43/434 (9%) Query: 197 KNRIAEKEPPHVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAME 256 K + AE ++ +L+ + PG++ + P G I + L Q++ + +YG E Sbjct: 22 KTKEAESSTQSNHVISSKQLLYTTDPTSPGSIFFLPHGTRILNKLIQFMKNQQTKYGFQE 81 Query: 257 VETPIMY------------DFEHPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMI 304 V TP+++ +++ + N +Y + Y L+ C MI Sbjct: 82 VVTPLIFKTKLWKKSGHWDNYKDDMFKVVGNDITKEEYTDNIEEHEYGLKPMNCPSHCMI 141 Query: 305 KKDAIISYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFK 364 SY+ LP+R + + R E G LSGL R+R F D H ++Q E K Sbjct: 142 FAKFDRSYQELPVRYSDFSSLH-RNEASGALSGLTRVRRFHQDDGHIFCS-MDQIDGEIK 199 Query: 365 KQFKLSMEVLEGVGLTPEDYEVAIRFTEDFWKEHKDFIV---ELVKLIGKPVLIEMWKQR 421 +L + G+ ++ ++ R + F E + + + +L ++ W R Sbjct: 200 NTLELIKDTYNVFGINEIEFYLSTRPEDKFIGEIETWDIAESQLKNVLNSTAGEGNWATR 259 Query: 422 F---FYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKY-PLILHCSP 477 ++ K + D +K + T+Q+D + RF + Y E+G+ + P+++H + Sbjct: 260 EGDGAFYGPKIDVLLKDAFNKKHQVGTIQLDFQLPNRFELKYVGEDGKRDHQPILVHRAV 319 Query: 478 SGAIERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAK 537 G++ER +L+ +GK +P W++P Q ++P++E ++ A + KL G Sbjct: 320 FGSLERFFAILLDHY-----QGK---WPFWINPRQALIVPINESHVPVAEELQKKLSGDI 371 Query: 538 I--------------RVDVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRRE 583 + VD+D E + + + A ++ Y++++G ++ NGT VR R+ Sbjct: 372 VSCDNAIAPLTGFTFHVDIDKRGETVGARTKDAIQKGYSYLIMIGDKDIANGTFAVRSRD 431 Query: 584 DGKQYETRIEELIK 597 D K +E+ K Sbjct: 432 DRKIKNMTSDEIYK 445 >gb|AAF97275.1|AC034106_18 (AC034106) Strong similarity to threonyl-tRNA synthetase from Arabidopsis thaliana gb|Y14329 and is a member of the tRNA synthetase PF|00587 class II Length = 458 Score = 116 bits (287), Expect = 9e-25 Identities = 91/303 (30%), Positives = 141/303 (46%), Gaps = 26/303 (8%) Query: 315 LPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVL 374 LP+RM E R E G L G+ R+R F D H + ++Q +EE K V Sbjct: 170 LPIRMAEFG-VLHRNEDSGALGGMTRVRRFVQDDAHIFCR-VDQVEEEVKGVLDFIDYVY 227 Query: 375 EGVGLTPEDYEVAIRFTE-------DFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFIL 427 G T YE+ + + W + ++ + + + GKP +I+ F+ Sbjct: 228 RIFGFT---YELTLSTRPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYG--P 282 Query: 428 KFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYA 487 K + D ++ +T+Q+D + F + Y E E + P+++H + G+IER+ Sbjct: 283 KIDITVSDARNRKFQCATIQLDFQLPACFKLKYLSEKNEMEAPVMIHSAVLGSIERMFAI 342 Query: 488 ILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDE 547 +LE K ++P WLSP Q V +SE+ YA V ++ VD+D+ D Sbjct: 343 LLEHY--------KGIWPFWLSPRQAIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDR 394 Query: 548 RLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRRED--GKQ--YETRIEELIKEIKEKT 603 L KK+ A YI+VVG +E G +TVR RED G++ E IE L+ E K KT Sbjct: 395 SLRKKVADARDAPYNYILVVGQKEVATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKT 454 Query: 604 EGF 606 F Sbjct: 455 VNF 457 >gi|6322654 mitochondrial threonine-tRNA synthetase; Mst1p [Saccharomyces cerevisiae] >gi|549032|sp|P07236|SYTM_YEAST THREONYL-TRNA SYNTHETASE, MITOCHONDRIAL PRECURSOR (THREONINE--TRNA LIGASE) (THRRS) >gi|538561|pir||YSBYTM threonine--tRNA ligase (EC 6.1.1.3) precursor, mitochondrial - yeast (Saccharomyces cerevisiae) >gi|486345|emb|CAA82038.1| (Z28194) ORF YKL194c [Saccharomyces cerevisiae] Length = 462 Score = 113 bits (279), Expect = 8e-24 Identities = 104/402 (25%), Positives = 176/402 (42%), Gaps = 51/402 (12%) Query: 225 PGNLRYYPKG-RLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYI 283 PG++ + P G ++ L+E ++ ++G EV TP++Y + + + Sbjct: 56 PGSMFFLPNGAKIFNKLIEFMKLQQKFKFGFNEVVTPLIYKKTLWEKSGHWENYADDMFK 115 Query: 284 VLSGDKR---YFLRFAACFGQFMI--KKDAIISYRNLPLRMYELTRYSFRREKRGELSGL 338 V + D+ Y L+ C G +I KKD SY LPLR + + R E G LSGL Sbjct: 116 VETTDEEKEEYGLKPMNCPGHCLIFGKKDR--SYNELPLRFSDFSPLH-RNEASGALSGL 172 Query: 339 RRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEV-------LEGVGLTPEDYEVAIRFT 391 RLR F D H Q K E KL V ++G +Y + Sbjct: 173 TRLRKFHQDDGHIFCTP-SQVKSEIFNSLKLIDIVYNKIFPFVKGGSGAESNYFINFSTR 231 Query: 392 EDF-------WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALS 444 D W + + E+++ GKP + F+ K + D+L K ++ Sbjct: 232 PDHFIGDLKVWNHAEQVLKEILEESGKPWKLNPGDGAFYG--PKLDIMVTDHLRKTHQVA 289 Query: 445 TVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMF 504 T+Q+D + ERF + + D++ K P+++H + G+IER M +++ NEG+ + Sbjct: 290 TIQLDFQLPERFDLKFKDQDNSYKRPIMIHRATFGSIERFMALLIDS-----NEGR---W 341 Query: 505 PLWLSPIQVRVIPVSE---EYLDYALYVAGKLEGA--------------KIRVDVDDEDE 547 P WL+P Q +IPV+ + LD + KL VD+D +E Sbjct: 342 PFWLNPYQAVIIPVNTKNVQQLDMCTALQKKLRNELEADDMEPVPLNDWHFNVDLDIRNE 401 Query: 548 RLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYE 589 + +I+ A + Y+++VG E + +R R++ K +E Sbjct: 402 PVGYRIKSAILKNYSYLIIVGDEEVQLQKYNIRERDNRKSFE 443 >gb|AAA34808.1| (M12087) thr-tRNA-synthetase [Saccharomyces cerevisiae] Length = 462 Score = 112 bits (278), Expect = 1e-23 Identities = 104/402 (25%), Positives = 176/402 (42%), Gaps = 51/402 (12%) Query: 225 PGNLRYYPKG-RLIKSLLEQYVTEKVIEYGAMEVETPIMYDFEHPALEKYLNRFPARQYI 283 PG++ + P G ++ L+E ++ ++G EV TP++Y + + + Sbjct: 56 PGSMFFLPNGAKIFNKLIEFMKLQQKFKFGFNEVVTPLIYKKTLWEKSGHWENYADDMFK 115 Query: 284 VLSGDKR---YFLRFAACFGQFMI--KKDAIISYRNLPLRMYELTRYSFRREKRGELSGL 338 V + D+ Y L+ C G +I KKD SY LPLR + + R E G LSGL Sbjct: 116 VETTDEEKEEYGLKPMNCPGHCLIFGKKDR--SYNELPLRFSDFSPLH-RNEASGALSGL 172 Query: 339 RRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEV-------LEGVGLTPEDYEVAIRFT 391 RLR F D H Q K E KL V ++G +Y + Sbjct: 173 TRLRKFHQDDGHIFCTP-SQVKSEIFNSLKLIDIVYNKIFPFVKGGSGAESNYFINFSTR 231 Query: 392 EDF-------WKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALS 444 D W + + E+++ GKP + F+ K + D+L K ++ Sbjct: 232 PDHFIGDLKVWNHAEQVLKEILEESGKPWKLNPSDGAFYG--PKLDIMVTDHLRKTHQVA 289 Query: 445 TVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMF 504 T+Q+D + ERF + + D++ K P+++H + G+IER M +++ NEG+ + Sbjct: 290 TIQLDFQLPERFDLKFKDQDNSYKRPIMIHRATFGSIERFMALLIDS-----NEGR---W 341 Query: 505 PLWLSPIQVRVIPVSE---EYLDYALYVAGKLEGA--------------KIRVDVDDEDE 547 P WL+P Q +IPV+ + LD + KL VD+D +E Sbjct: 342 PFWLNPYQAVIIPVNTKNVQQLDMCTALQKKLRNELEADDMEPVPLNDWHFNVDLDIRNE 401 Query: 548 RLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYE 589 + +I+ A + Y+++VG E + +R R++ K +E Sbjct: 402 PVGYRIKSAILKNYSYLIIVGDEEVQLQKYNIRERDNRKSFE 443 >emb|CAA26666.1| (X02906) threonyl-tRNA synthetase (aa 1-734) [Saccharomyces cerevisiae] Length = 734 Score = 111 bits (274), Expect = 3e-23 Identities = 107/406 (26%), Positives = 180/406 (43%), Gaps = 26/406 (6%) Query: 207 HVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE 266 H K+ E EL + S PG+ + P G I + L + + + G EV TP MY+ + Sbjct: 330 HRKIGKEQELFLFNEMS-PGSCFWLPHGTRIYNTLVDLLRTEYRKRGYEEVITPNMYNSK 388 Query: 267 HPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYS 326 + + + + + L+ C G ++ K SYR LP R+ + Sbjct: 389 LWETSGHWANYKENMFTFEVEKETFGLKPMNCPGHCLMFKSRERSYRELPWRVADFG-VI 447 Query: 327 FRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEV 386 R E G LSGL R+R F D H +Q + E + F ++ + GV + E+ Sbjct: 448 HRNEFSGALSGLTRVRRFQQDDAHIFCTH-DQIESEIENIFNF-LQYIYGVFGFEFEMEL 505 Query: 387 AIRFTE-----DFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAA 441 + R + + W + + +K G I F+ K + D L + Sbjct: 506 STRPEKYVGKIETWDAAESKLESALKKWGGNWEINAGDGAFYG--PKIDIMISDALRRWH 563 Query: 442 ALSTVQIDVENAERFGITYYDENGE-EKY--PLILHCSPSGAIERVMYAILEKQAKLMNE 498 +T+Q+D + RF + + ++ + E Y P+++H + G++ER+ + E A Sbjct: 564 QCATIQLDFQLPNRFELEFKSKDQDSESYERPVMIHRAILGSVERMTAILTEHFA----- 618 Query: 499 GKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEK 558 GK +P WLSP QV V+PV +Y YA V KL A DVD L KK+R + Sbjct: 619 GK---WPFWLSPRQVLVVPVGVKYQGYAEDVRNKLHDAGFYADVDLTGNTLQKKVRNGQM 675 Query: 559 EWIPYIVVVGAREKENGTITVRRR----EDGKQYETRIEELIKEIK 600 +I +VG +E ++ +R R + GK +EE++K+++ Sbjct: 676 LKYNFIFIVGEQEMNEKSVNIRNRDVMEQQGKNATVSVEEVLKQLR 721 >gi|6322112 Threonyl-tRNA synthetase, cytoplasmic; Ths1p [Saccharomyces cerevisiae] >gi|730881|sp|P04801|SYTC_YEAST THREONYL-TRNA SYNTHETASE, CYTOPLASMIC (THREONINE--TRNA LIGASE) (THRRS) >gi|625247|pir||YSBYTC threonine--tRNA ligase (EC 6.1.1.3), cytosolic - yeast (Saccharomyces cerevisiae) >gi|556869|emb|CAA86092.1| (Z37997) threonyl-tRNA synthetase [Saccharomyces cerevisiae] Length = 734 Score = 111 bits (274), Expect = 3e-23 Identities = 109/410 (26%), Positives = 178/410 (42%), Gaps = 34/410 (8%) Query: 207 HVKLMLEHELVDYEPGSDPGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE 266 H K+ E EL + S PG+ + P G I + L + + + G EV TP MY+ + Sbjct: 330 HRKIGKEQELFLFNEMS-PGSCFWLPHGTRIYNTLVDLLRTEYRKRGYEEVITPNMYNSK 388 Query: 267 HPALEKYLNRFPARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYS 326 + + + + + L+ C G ++ K SYR LP R+ + Sbjct: 389 LWETSGHWANYKENMFTFEVEKETFGLKPMNCPGHCLMFKSRERSYRELPWRVADFG-VI 447 Query: 327 FRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGV--------- 377 R E G LSGL R+R F D H +Q + E + F ++ + GV Sbjct: 448 HRNEFSGALSGLTRVRRFQQDDAHIFCTH-DQIESEIENIFNF-LQYIYGVFGFEFKMEL 505 Query: 378 GLTPEDYEVAIRFTEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNL 437 PE Y I + W + + +K G I F+ K + D L Sbjct: 506 STRPEKYVGKI----ETWDAAESKLESALKKWGGNWEINAGDGAFYG--PKIDIMISDAL 559 Query: 438 DKAAALSTVQIDVENAERFGITYYDENGE-EKY--PLILHCSPSGAIERVMYAILEKQAK 494 + +T+Q+D + RF + + ++ + E Y P+++H + G++ER+ + E A Sbjct: 560 RRWHQCATIQLDFQLPNRFELEFKSKDQDSESYERPVMIHRAILGSVERMTAILTEHFA- 618 Query: 495 LMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIR 554 GK +P WLSP QV V+PV +Y YA V KL A DVD L KK+R Sbjct: 619 ----GK---WPFWLSPRQVLVVPVGVKYQGYAEDVRNKLHDAGFYADVDLTGNTLQKKVR 671 Query: 555 RAEKEWIPYIVVVGAREKENGTITVRRR----EDGKQYETRIEELIKEIK 600 + +I +VG +E ++ +R R + GK +EE++K+++ Sbjct: 672 NGQMLKYNFIFIVGEQEMNEKSVNIRNRDVMEQQGKNATVSVEEVLKQLR 721 >dbj|BAB14117.1| (AK022590) unnamed protein product [Homo sapiens] Length = 588 Score = 102 bits (253), Expect = 9e-21 Identities = 83/299 (27%), Positives = 140/299 (46%), Gaps = 23/299 (7%) Query: 311 SYRNLPLRMYELTRYSFRREKRGELSGLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLS 370 S+R LPLR+ + R E G L GL RLR F D H +Q + E + Sbjct: 292 SWRELPLRLADFGALH-RAEASGGLGGLTRLRCFQQDDAHIFCTT-DQLEAEIQSCLDFL 349 Query: 371 MEVLEGVGLTPEDYEVAIR-----FTED--FWKEHKDFIVELVKLIGKPVLIEMWKQRFF 423 V +G + + +A+ F D W + + + + +K G+P + F+ Sbjct: 350 RSVYAVLGFS---FRLALSTRPSGFLGDPCLWDQAEQVLKQALKEFGEPWDLNSGDGAFY 406 Query: 424 YFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIER 483 K + + D L + T+Q+D + RF + Y + G + P+++H + G++ER Sbjct: 407 G--PKIDVHLHDALGRPHQCGTIQLDFQLPLRFDLQYKGQAGALERPVLIHRAVLGSVER 464 Query: 484 VMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVD 543 ++ + E GK +PLWLSP QV VIPV E +YA L A + D+D Sbjct: 465 LLGVLAESCG-----GK---WPLWLSPFQVVVIPVGSEQEEYAKEAQQSLRAAGLVSDLD 516 Query: 544 -DEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIKE 601 D L+++IRRA+ + VVG +E+ T+ +R R++ + E + E ++ + E Sbjct: 517 ADSGLTLSRRIRRAQLAHYNFQFVVGQKEQSKRTVNIRTRDNRRLGEWDLPEAVQRLVE 575 >sp|O13969|SYTM_SCHPO PROBABLE THREONYL-TRNA SYNTHETASE, MITOCHONDRIAL PRECURSOR (THREONINE--TRNA LIGASE) (THRRS) >gi|7493478|pir||T38350 threonine-tRNA ligase precursor, mitochondrial - fission yeast (Schizosaccharomyces pombe) >gi|2330792|emb|CAB11266.1| (Z98601) mitochondrial threonyl trna synthetase [Schizosaccharomyces pombe] Length = 473 Score = 98.7 bits (242), Expect = 2e-19 Identities = 100/420 (23%), Positives = 178/420 (41%), Gaps = 50/420 (11%) Query: 225 PGNLRYYPKGRLIKSLLEQYVTEKVIEYGAMEVETPIMYDFE-------HPALEKYLNRF 277 PG++ + P G I + L ++ + +G E+ TP+++ + EK + R Sbjct: 65 PGSIFFLPHGTRIYNRLVDFLRAQYQIHGFEEIITPLIFKKDLWEKSGHWQNYEKEIFRV 124 Query: 278 P-ARQYIVLSGDKRYFLRFAACFGQFMIKKDAIISYRNLPLRMYELTRYSFRREKRGELS 336 +R + Y L+ C G ++ SY+ LPLR + + R E G LS Sbjct: 125 EESRNVEDEHSNATYGLKPMNCPGHCIVYASTERSYKELPLRFADFSPLH-RNEASGALS 183 Query: 337 GLRRLRAFTMPDMHTLAKDIEQAKEEFKKQFKLSMEVLEGVGLTPEDYEVAIRFTE---- 392 GL RLR F D H E K+E K +V +G+ ++ R E Sbjct: 184 GLTRLRCFHQDDGHIFCSP-ESIKDEIKNTLTFVKQVYSLLGMNKLKLYLSTRPEEHIGS 242 Query: 393 -DFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVE 451 D W E ++ + E ++ G+ +I F+ K + D K +T+Q+D Sbjct: 243 LDTWNEAENGLREALQESGETWIINEGDGAFYG--PKIDVMVADARGKWHQTATIQLDFN 300 Query: 452 NAERFGITYYDENGEE-------KYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMF 504 +RF + Y + G+ K P+++H + G++ER M ++E + Sbjct: 301 LPQRFKLYYRTDAGDNAGSEKLIKQPVMIHRAIFGSLERFMGILIEHL--------NGHW 352 Query: 505 PLWLSPIQVRVIPV--SEEYLDYALYVAGKLEGAKIR-------------VDVDDEDERL 549 P WLSP ++PV ++ L YA V +L ++ VD++ E + + Sbjct: 353 PFWLSPRHAVILPVNQTDRILTYAKQVQKELSNQEVNESEFLPLNKNTYYVDINAEAQSI 412 Query: 550 NKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETR---IEELIKEIKEKTEGF 606 K++R + Y +V+G E + ++V R + +TR I++L KE E + + Sbjct: 413 GKRLRESRLLNYNYEIVIGEDEVDKEILSVSSRHNHNSRDTRKMTIQQLKKEFIENVKAY 472 >sp|Q9YFY3|SYT2_AERPE PROBABLE THREONYL-TRNA SYNTHETASE 2 (THREONINE--TRNA LIGASE 2) (THRRS 2) >gi|7521473|pir||B72766 probable threonyl-tRNA synthetase APE0117 - Aeropyrum pernix (strain K1) >gi|5103507|dbj|BAA79028.1| (AP000058) 406aa long hypothetical threonyl-tRNA synthetase [Aeropyrum pernix] Length = 406 Score = 85.0 bits (207), Expect = 2e-15 Identities = 53/121 (43%), Positives = 73/121 (59%), Gaps = 7/121 (5%) Query: 19 ALKNPEPISEDMKRGRMEEVLVAFISVEKVDEKNPEEVSLKAIEEISKVAEQVKAENVFV 78 ALKNP + G E LV F +VE D P+ V + A +I+ + ++K V + Sbjct: 4 ALKNPPDPPGEASFG---EALVVFTTVE--DGDGPQTV-MYAASDIASHSSRLKVTTVIL 57 Query: 79 YPFAHLSSELAKPSVAMDILNRVYQGLKER-GFNVGKAPFGYYKAFKISCKGHPLAELSR 137 YP+AHLSS LAKP A L + L+ + +V +APFG+YK+F I+CKGHPLAELSR Sbjct: 58 YPYAHLSSRLAKPMAAHKRLIELEGALRTKFPGHVHRAPFGWYKSFSIACKGHPLAELSR 117 Query: 138 T 138 + Sbjct: 118 S 118 Score = 73.7 bits (178), Expect = 6e-12 Identities = 43/113 (38%), Positives = 60/113 (53%), Gaps = 2/113 (1%) Query: 494 KLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKI 553 K EG P L+P+Q VIPV E YA +A L + +RV + L +++ Sbjct: 285 KARKEGLTPYLNPTLTPVQSAVIPVDSESEGYAQRIAEDLAASGVRVSIV-RGSGLGRRV 343 Query: 554 RRAEKEWIPYIVVVGAREKENGTITVRRR-EDGKQYETRIEELIKEIKEKTEG 605 R A + W ++VVG RE+E GT+ VRRR E GKQ ++EL E K+ G Sbjct: 344 REAGRSWASLVIVVGKREEETGTVVVRRRWEPGKQEVLTLDELSSEAKKLASG 396 >sp|P52833|SYT_PSESY THREONYL-TRNA SYNTHETASE (THREONINE--TRNA LIGASE) (THRRS) >gi|1171431|gb|AAB05013.1| (U44118) threonyl-tRNA synthetase [Pseudomonas syringae pv. syringae] Length = 227 Score = 84.7 bits (206), Expect = 3e-15 Identities = 54/193 (27%), Positives = 94/193 (47%), Gaps = 10/193 (5%) Query: 391 TEDFWKEHKDFIVELVKLIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDV 450 +++ W + + + G+P ++ + F+ K EF+ D L + T+Q+D Sbjct: 16 SDELWDRAESALASALDSAGQPYDLQPGEGAFYG--PKIEFSLKDCLGRVWQCGTLQLDF 73 Query: 451 ENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMFPLWLSP 510 R Y E+ K P++LH + G+ ER + ++E EG FP WL+P Sbjct: 74 NLPIRLSAEYVSEDNSRKNPVMLHRAILGSFERFIGILIEHY-----EGA---FPAWLAP 125 Query: 511 IQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYIVVVGAR 570 Q ++ ++++ D+AL V L + R D +E++ KIR +PY++V+G R Sbjct: 126 TQAVIMNITDKQADFALEVEKTLAESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDR 185 Query: 571 EKENGTITVRRRE 583 E E T+ VR RE Sbjct: 186 EVEMQTVAVRTRE 198 >gb|AAD34790.1|AF136400_4 (AF136400) threonyl-tRNA synthetase [Pseudomonas fluorescens] Length = 161 Score = 79.6 bits (193), Expect = 1e-13 Identities = 47/139 (33%), Positives = 74/139 (52%), Gaps = 8/139 (5%) Query: 445 TVQIDVENAERFGITYYDENGEEKYPLILHCSPSGAIERVMYAILEKQAKLMNEGKKPMF 504 T+Q+D R G Y E+ K+P++LH + G+ ER + ++E EG F Sbjct: 2 TLQLDFNLPIRLGAEYVSEDNSRKHPVMLHRAILGSFERFVGILIEHY-----EGA---F 53 Query: 505 PLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRVDVDDEDERLNKKIRRAEKEWIPYI 564 P WL+P Q V+ ++++ D+AL V L + R D +E++ KIR +PY+ Sbjct: 54 PAWLAPTQAVVMNITDKQADFALEVEKTLNQSGFRAKSDLRNEKIGFKIREHTLLKVPYL 113 Query: 565 VVVGAREKENGTITVRRRE 583 +V+G RE E T+ VR RE Sbjct: 114 LVIGDREVEMQTVAVRTRE 132 >gb|AAF78372.1|AC069551_5 (AC069551) T10O22.10 [Arabidopsis thaliana] Length = 270 Score = 79.6 bits (193), Expect = 1e-13 Identities = 63/186 (33%), Positives = 90/186 (47%), Gaps = 22/186 (11%) Query: 422 FFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDENGEE-KYPLILHCSPSGA 480 FF+F F A L+ +D +RF + Y EN + +++H + + Sbjct: 105 FFFFFFLFP---------AFLLAVYLVDFHLRDRFKLEYSAENETMIERSVLIHRAVLSS 155 Query: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 +ER ++AIL +Q K GK FP WLSP Q V VSE+Y YA V ++ A V Sbjct: 156 VER-LFAILLEQYK----GK---FPFWLSPRQAIVCSVSEDYRSYADKVRDQIHEAGYYV 207 Query: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK 600 DVD D ++ K++ A+ YI+VVG E G +TV R++ ET L E K Sbjct: 208 DVDTTDRNVSDKVQEAQVAHYNYILVVGDEEARTGQVTVWLRDEMMSIET----LSDEFK 263 Query: 601 EKTEGF 606 KT F Sbjct: 264 LKTANF 269 >gb|AAF97829.1|AC034107_12 (AC034107) Contains similarity to threonyl-tRNA synthetase from Arabidopsis thaliana gb|Y14329 Length = 130 Score = 73.0 bits (176), Expect = 9e-12 Identities = 46/126 (36%), Positives = 64/126 (50%), Gaps = 4/126 (3%) Query: 481 IERVMYAILEKQAKLMNEGKKPMFPLWLSPIQVRVIPVSEEYLDYALYVAGKLEGAKIRV 540 I R + + +E+ ++ E K FP WLSP Q V VSE+Y YA V ++ A V Sbjct: 8 IHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAIVCSVSEDYRSYADKVRDQIHEAGYYV 67 Query: 541 DVDDEDERLNKKIRRAEKEWIPYIVVVGAREKENGTITVRRREDGKQYETRIEELIKEIK 600 DVD D ++ K++ A+ YI+VVG E G +TV R++ ET L E K Sbjct: 68 DVDTTDRNVSDKVQEAQVAHYNYILVVGDEEARTGQVTVWLRDEMMSIET----LSDEFK 123 Query: 601 EKTEGF 606 KT F Sbjct: 124 LKTANF 129 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.319 0.139 0.399 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 239413458 Number of Sequences: 2977 Number of extensions: 10614569 Number of successful extensions: 33130 Number of sequences better than 1.0e-10: 67 Number of HSP's better than 0.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 34 Number of HSP's that attempted gapping in prelim test: 32843 Number of HSP's gapped (non-prelim): 80 length of query: 625 length of database: 189,106,746 effective HSP length: 58 effective length of query: 567 effective length of database: 154,394,500 effective search space: 87541681500 effective search space used: 87541681500 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 168 (69.9 bits)