BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1501 (dph5) DE:diphthine synthase (dph5)
(262 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||H75041 diphthine synthase (dph5) PAB1501 - Pyrococcus abyss... 522 e-147
pir||F71119 probable diphthine synthase - Pyrococcus horikoshii ... 466 e-130
pir||A64459 diphthine synthase (EC 2.1.1.98) - Methanococcus jan... 288 4e-77
emb|CAA73259.1| (Y12727) diphthine synthase [Pyrococcus furiosus] 277 1e-73
pir||G69117 diphthine synthase - Methanobacterium thermoautotrop... 267 8e-71
pir||T41424 probable diphthine synthase - fission yeast (Schizo... 196 2e-49
gb|AAG19507.1| (AE005042) L-lactate permease; Lds [Halobacterium... 191 8e-48
pir||T05091 probable methyltransferase F28M20.20 - Arabidopsis t... 188 7e-47
gi|6323201 S-adenosylmethionine (AdoMet)-dependent methyltransfe... 184 8e-46
gi|7706272 CGI-30 protein [Homo sapiens] >gi|4680699|gb|AAD27739... 178 8e-44
gb|AAD46869.1|AF160929_1 (AF160929) BcDNA.LD12153 [Drosophila me... 174 1e-42
gb|AAF56023.1| (AE003739) Dph5 gene product [Drosophila melanoga... 174 1e-42
gi|11497993 diphthine synthase (dph5) [Archaeoglobus fulgidus] >... 173 2e-42
pir||T18768 hypothetical protein B0491.7 - Caenorhabditis elegan... 170 1e-41
gb|AAK41227.1| Diphthine synthase, hypothetical [Sulfolobus solf... 157 1e-37
gb|AAF67485.1|AF157319_1 (AF157319) AD-018 protein [Homo sapiens] 118 5e-26
gb|AAF29107.1|AF161492_1 (AF161492) HSPC143 [Homo sapiens] 116 2e-25
emb|CAC12012.1| (AL445065) methyltransferase DPH5 related protei... 112 5e-24
gi|11422835 CGI-30 protein [Homo sapiens] 106 2e-22
pir||C72689 probable diphtine synthase APE0931 - Aeropyrum perni... 93 3e-18
>pir||H75041 diphthine synthase (dph5) PAB1501 - Pyrococcus abyssi (strain
Orsay) >gi|5458742|emb|CAB50229.1| (AJ248287) diphthine
synthase (dph5) [Pyrococcus abyssi]
Length = 262
Score = 522 bits (1329), Expect = e-147
Identities = 262/262 (100%), Positives = 262/262 (100%)
Query: 1 MALYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSR 60
MALYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSR
Sbjct: 1 MALYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSR 60
Query: 61 EDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAI 120
EDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAI
Sbjct: 61 EDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAI 120
Query: 121 AITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTANE 180
AITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTANE
Sbjct: 121 AITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTANE 180
Query: 181 GMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPG 240
GMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPG
Sbjct: 181 GMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPG 240
Query: 241 RLHIVEAEYLVEIAGAPREIIR 262
RLHIVEAEYLVEIAGAPREIIR
Sbjct: 241 RLHIVEAEYLVEIAGAPREIIR 262
>pir||F71119 probable diphthine synthase - Pyrococcus horikoshii
>gi|3257133|dbj|BAA29816.1| (AP000003) 265aa long
hypothetical diphthine synthase [Pyrococcus horikoshii]
Length = 265
Score = 466 bits (1186), Expect = e-130
Identities = 228/262 (87%), Positives = 246/262 (93%)
Query: 1 MALYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSR 60
M LYFIGLGLYDE+DIT+KGLEIA+KCDYVFAEFYTSLMAGT L RI+KLIGKEIRVLSR
Sbjct: 1 MVLYFIGLGLYDERDITVKGLEIAKKCDYVFAEFYTSLMAGTTLGRIQKLIGKEIRVLSR 60
Query: 61 EDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAI 120
EDVELNFE IVLPLAK++DVAFLT GDPLVATTHAELR+RAK+ GVESYVIHAPSIYSA+
Sbjct: 61 EDVELNFENIVLPLAKENDVAFLTPGDPLVATTHAELRIRAKRAGVESYVIHAPSIYSAV 120
Query: 121 AITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTANE 180
ITGLHIYKFGKSAT++YPE NWFPTSYYDVIKEN ERGLHTLLFLDIKAEK YMTANE
Sbjct: 121 GITGLHIYKFGKSATVAYPEGNWFPTSYYDVIKENAERGLHTLLFLDIKAEKRMYMTANE 180
Query: 181 GMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPG 240
M+LLLKVEDMKK GVFT +TLVVVLARAGSLNP IRAGYV+D+IREDFG PPH+LIVPG
Sbjct: 181 AMELLLKVEDMKKGGVFTDDTLVVVLARAGSLNPTIRAGYVKDLIREDFGDPPHILIVPG 240
Query: 241 RLHIVEAEYLVEIAGAPREIIR 262
+LHIVEAEYLVEIAGAPREI+R
Sbjct: 241 KLHIVEAEYLVEIAGAPREILR 262
>pir||A64459 diphthine synthase (EC 2.1.1.98) - Methanococcus jannaschii
>gi|1591910|gb|AAB99280.1| (U67568) diphthine synthase
(dph5) [Methanococcus jannaschii]
Length = 257
Score = 288 bits (730), Expect = 4e-77
Identities = 137/250 (54%), Positives = 194/250 (76%), Gaps = 3/250 (1%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
L GLGLYDE D+TLK L+ A+K + ++AEFYT+++ GT E+IE+++GK+I VLSR+D
Sbjct: 2 LILAGLGLYDENDMTLKTLKFAKKAEKIYAEFYTAVLTGTTTEKIEEVLGKKIHVLSRKD 61
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAI 122
VE N +++ AKD D+ FLTAGDP+VATTH +L + AKK G+E +I+APSIYSA+ I
Sbjct: 62 VEYNGYKLIEE-AKDKDIMFLTAGDPMVATTHVDLAIEAKKKGIEVLIINAPSIYSAVGI 120
Query: 123 TGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKG--KYMTANE 180
TGL +YKFGK+ +I +PE+N+FP + Y+VIKENLERGLHTL LDI+ ++ ++MTANE
Sbjct: 121 TGLQLYKFGKTTSIVFPEENYFPETPYNVIKENLERGLHTLCLLDIRIDENEKRFMTANE 180
Query: 181 GMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPG 240
G+K+LL++E+ KKEG+ ++T VV+ARAGSL P + G ++D+I DFG P H +I+PG
Sbjct: 181 GLKVLLELENRKKEGIINEDTKAVVVARAGSLKPKLVYGKIKDLINYDFGEPLHCIIIPG 240
Query: 241 RLHIVEAEYL 250
+LH +E + L
Sbjct: 241 KLHFMEEDAL 250
>emb|CAA73259.1| (Y12727) diphthine synthase [Pyrococcus furiosus]
Length = 213
Score = 277 bits (701), Expect = 1e-73
Identities = 133/161 (82%), Positives = 152/161 (93%)
Query: 38 LMAGTNLERIEKLIGKEIRVLSREDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAEL 97
LMAGT LE+IE++IGK+I VL+REDVE+NFE+IVLP AK+ +VAFLTAGDP+VATTHAEL
Sbjct: 1 LMAGTTLEKIEEVIGKKIIVLNREDVEMNFEKIVLPEAKEKEVAFLTAGDPMVATTHAEL 60
Query: 98 RLRAKKFGVESYVIHAPSIYSAIAITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLE 157
R+RAK+ GVESYVIHAPSIYSA+AITGLHIYKFGKSAT++YPE NWFPTSYYDV+KENLE
Sbjct: 61 RIRAKRMGVESYVIHAPSIYSAVAITGLHIYKFGKSATVAYPEGNWFPTSYYDVVKENLE 120
Query: 158 RGLHTLLFLDIKAEKGKYMTANEGMKLLLKVEDMKKEGVFT 198
RGLHTLLFLDIKAEKGKYMTANE M+LLLKVE+M+ E VFT
Sbjct: 121 RGLHTLLFLDIKAEKGKYMTANEAMELLLKVEEMRGENVFT 161
>pir||G69117 diphthine synthase - Methanobacterium thermoautotrophicum (strain
Delta H) >gi|2623012|gb|AAB86340.1| (AE000940) diphthine
synthase [Methanobacterium thermoautotrophicum]
Length = 264
Score = 267 bits (676), Expect = 8e-71
Identities = 134/260 (51%), Positives = 181/260 (69%), Gaps = 3/260 (1%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
LY IGLGLYDE DI++KGL CD V+AEFYT+++ G++L IE+ IG+ ++VL R +
Sbjct: 2 LYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAMLQGSSLSAIERRIGRSVKVLGRGE 61
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAI-A 121
+E ERI + A + DVA L AGDPLVATTH EL + A + G+E+ VIH+ SI SA
Sbjct: 62 IEE--ERIPIERALELDVALLVAGDPLVATTHTELLIDAHRRGIETRVIHSSSIISAAPG 119
Query: 122 ITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTANEG 181
+ GL YKFG+ TI + N+FPTS Y I ENL+ G H+L+ LDI+A + +YMTA EG
Sbjct: 120 LAGLQAYKFGRIITIPFTSDNYFPTSPYTNIGENLKAGSHSLVLLDIEAHRNRYMTATEG 179
Query: 182 MKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPGR 241
++ LLK + +G + ETL VV+ARAGS P++RA + ++ EDFG P H L+VP
Sbjct: 180 LRYLLKASEKLDDGTISGETLAVVIARAGSEKPLVRADKIATLVEEDFGDPLHCLVVPAG 239
Query: 242 LHIVEAEYLVEIAGAPREII 261
LH +EA+YLVEIAGAPR ++
Sbjct: 240 LHFIEADYLVEIAGAPRAVV 259
>pir||T41424 probable diphthine synthase - fission yeast (Schizosaccharomyces
pombe) >gi|3687469|emb|CAA21193.1| (AL031798) putative
diphthine synthase [Schizosaccharomyces pombe]
Length = 283
Score = 196 bits (493), Expect = 2e-49
Identities = 109/263 (41%), Positives = 166/263 (62%), Gaps = 18/263 (6%)
Query: 4 YFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSREDV 63
Y IGLGL+DEKDITL+GLE +KC ++ E YTS++ E++E+L GKE+ + RE V
Sbjct: 3 YLIGLGLFDEKDITLRGLETVKKCQRIYLEAYTSILL-VQKEKLEELYGKEVILADREMV 61
Query: 64 ELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAIT 123
E + + I L A + DVA L GDP+ ATTHA+L +RA++ + +IH SI +AI
Sbjct: 62 ESSSDEI-LKDADNCDVAMLVVGDPMGATTHADLVIRARELKIPVRMIHNASIMNAIGAC 120
Query: 124 GLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKA------------- 170
GL +YKFG++ ++ + E N+ P S+YD IKEN+ GLHTL+ LDIK
Sbjct: 121 GLQLYKFGQTVSLVFFENNYRPQSFYDHIKENVSLGLHTLVLLDIKVKEQSWENLARGRK 180
Query: 171 --EKGKYMTANEGMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIRED 228
E +YM+A+ + +L+VE+ ++E + T ++L V + R GS + VI AG ++++ D
Sbjct: 181 VYEPPRYMSASLAAQQMLEVEEDRQENICTPDSLCVAVGRMGSDDQVIFAGTLQELAEHD 240
Query: 229 FGSPPHVLIVPGR-LHIVEAEYL 250
G P H +++ GR +H +E E+L
Sbjct: 241 IGPPLHSVVLVGRDVHDLELEFL 263
>gb|AAG19507.1| (AE005042) L-lactate permease; Lds [Halobacterium sp. NRC-1]
Length = 282
Score = 191 bits (480), Expect = 8e-48
Identities = 115/268 (42%), Positives = 160/268 (58%), Gaps = 18/268 (6%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
L F+GLGLYDE +T+ G + D VFAEFYTS + GT++ +E I R
Sbjct: 24 LTFVGLGLYDEASVTVAGRDAIAAADRVFAEFYTSRLIGTDVAALEAHHDTTIERRDRAG 83
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIA- 121
VE + E I L A D D FLTAGD +++TTH +LR+RA G+++ VIHAP+ SA A
Sbjct: 84 VEQHPEPI-LDAAADGDAVFLTAGDTMISTTHVDLRMRAADRGIDTRVIHAPTAASAAAG 142
Query: 122 ITGLHIYKFGKSATISYP---EKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGK---- 174
+TGL Y+FGK+ T+ +P + P S D I+ N ERGLHTL++LDIK + +
Sbjct: 143 LTGLQNYRFGKATTLPFPWAHGADGVPGSVTDTIEANRERGLHTLVYLDIKVDHPRVDGD 202
Query: 175 -YMTANEGMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPP 233
YMTA++ LL D + L VV+ARAG+ + +RA + + DFGSP
Sbjct: 203 AYMTASQAADLLATNWDA--------DALGVVVARAGAPDATVRADRLGALADADFGSPL 254
Query: 234 HVLIVPGRLHIVEAEYLVEIAGAPREII 261
H+L+VPG LH +E + L E+AGAP + +
Sbjct: 255 HLLVVPGSLHHIERDALRELAGAPADAL 282
>pir||T05091 probable methyltransferase F28M20.20 - Arabidopsis thaliana
>gi|3281849|emb|CAA19744.1| (AL031004) methyltransferase
- like protein [Arabidopsis thaliana]
>gi|7270082|emb|CAB79897.1| (AL161579)
methyltransferase-like protein [Arabidopsis thaliana]
Length = 277
Score = 188 bits (472), Expect = 7e-47
Identities = 109/268 (40%), Positives = 154/268 (56%), Gaps = 21/268 (7%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMA----GTNLERIEKLIGKEIRVL 58
LY IGLGL DEKDITL+GLE +K V+ E YTSL++ L +EK GK I +
Sbjct: 2 LYIIGLGLGDEKDITLRGLEAVKKSQKVYMEAYTSLLSFGLSADGLSNLEKFYGKPIILA 61
Query: 59 SREDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYS 118
RE VE ++ A D+DVAFL GDP ATTH++L +RAK GV+ V+H S+ +
Sbjct: 62 DREMVEEKAGDMI-DEAIDNDVAFLVVGDPFGATTHSDLVVRAKTLGVKVEVVHNASVMN 120
Query: 119 AIAITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKA-------- 170
A+ I GL +Y +G++ +I + + W P S+Y+ IK+N GLHTL LDI+
Sbjct: 121 AVGICGLQLYHYGETVSIPFFTETWRPDSFYEKIKKNRSLGLHTLCLLDIRVKEPTFESL 180
Query: 171 --------EKGKYMTANEGMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVR 222
E +YM+ N ++ LL+VE + V+ ++T V AR GS + I AG ++
Sbjct: 181 CRGGKKQYEPPRYMSVNTAIEQLLEVEQKHGDSVYGEDTQCVGFARLGSEDQTIVAGTMK 240
Query: 223 DMIREDFGSPPHVLIVPGRLHIVEAEYL 250
+ DFG+P H L++ G H VE E L
Sbjct: 241 QLESVDFGAPLHCLVIVGETHPVEEEML 268
>gi|6323201 S-adenosylmethionine (AdoMet)-dependent methyltransferase of
diphthamide biosynthesis; Dph5p [Saccharomyces
cerevisiae] >gi|416912|sp|P32469|DPH5_YEAST DIPHTHINE
SYNTHASE (DIPHTAMIDE BIOSYNTHESIS METHYLTRANSFERASE)
>gi|320791|pir||S30890 methyltransferase DPH5
[validated] - yeast (Saccharomyces cerevisiae)
>gi|171416|gb|AAA34577.1| (M83375) DPH5 [Saccharomyces
cerevisiae] >gi|577209|gb|AAB67469.1| (U17246) Dph5p:
diphthine synthase [Saccharomyces cerevisiae]
Length = 300
Score = 184 bits (463), Expect = 8e-46
Identities = 104/268 (38%), Positives = 154/268 (56%), Gaps = 17/268 (6%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
LY IGLGL + DIT++GLE +KC V+ E YTS++ + E +E GKEI + RE
Sbjct: 2 LYLIGLGLSYKSDITVRGLEAIKKCSRVYLEHYTSILMAASQEELESYYGKEIILADREL 61
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAI 122
VE ++I L A DVAFL GDP ATTH +L LRAK+ + +IH S+ +A+
Sbjct: 62 VETGSKQI-LNNADKEDVAFLVVGDPFGATTHTDLVLRAKREAIPVEIIHNASVMNAVGA 120
Query: 123 TGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKA------------ 170
GL +Y FG++ ++ + NW P S+YD I EN + GLHTL+ LDIK
Sbjct: 121 CGLQLYNFGQTVSMVFFTDNWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR 180
Query: 171 ---EKGKYMTANEGMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIRE 227
E +YM+ + + LL++E+ + +T +T V ++R GS + ++G + ++
Sbjct: 181 LIYEPPRYMSIAQCCEQLLEIEEKRGTKAYTPDTPAVAISRLGSSSQSFKSGTISELANY 240
Query: 228 DFGSPPHVLIVPGR-LHIVEAEYLVEIA 254
D G P H L++ GR H +E EYL+E A
Sbjct: 241 DSGEPLHSLVILGRQCHELELEYLLEFA 268
>gi|7706272 CGI-30 protein [Homo sapiens] >gi|4680699|gb|AAD27739.1|AF132964_1
(AF132964) CGI-30 protein [Homo sapiens]
Length = 297
Score = 178 bits (446), Expect = 8e-44
Identities = 111/280 (39%), Positives = 154/280 (54%), Gaps = 34/280 (12%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
LY IGLGL D KDIT+KGLE+ R+C V+ E YTS++ E +E+ G+++ V RE+
Sbjct: 2 LYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLT-VGKEALEEFYGRKLVVADREE 60
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAI 122
VE + I L A DVAFL GDP ATTH++L LRA K G+ VIH SI +A+
Sbjct: 61 VEQEADNI-LKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGC 119
Query: 123 TGLHIYKFGKSATISYPE------------KNWFPTSYYDVIKENLERGLHTLLFLDIKA 170
GL +YKFG++ +I W P S++D +K+N + G+HTL LDIK
Sbjct: 120 CGLQLYKFGETVSIMLISVMLHSLWLVIHLDTWRPESFFDKVKKNRQNGMHTLCLLDIKV 179
Query: 171 ---------------EKGKYMTANEGMKLLLKVEDMKK----EGVFTQETLVVVLARAGS 211
E +YM+ N+ + LL++ ++ E T+ETL V LAR G+
Sbjct: 180 KEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGA 239
Query: 212 LNPVIRAGYVRDMIREDFGSPPHVLIVP-GRLHIVEAEYL 250
+ I AG +R M D G P H LI+ G +H +E E L
Sbjct: 240 DDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEML 279
>gb|AAD46869.1|AF160929_1 (AF160929) BcDNA.LD12153 [Drosophila melanogaster]
Length = 281
Score = 174 bits (436), Expect = 1e-42
Identities = 105/271 (38%), Positives = 153/271 (55%), Gaps = 22/271 (8%)
Query: 4 YFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSREDV 63
Y IGLGL D KDIT+KGLEI ++C V+ E YTS++ G +LE +++ G+ + + R+ V
Sbjct: 3 YLIGLGLGDLKDITVKGLEIVKQCSRVYLEMYTSIL-GCSLEDMQEFYGRPLLLADRDLV 61
Query: 64 ELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAIT 123
E + I L A + DVA L GDP ATTH + LRAK+ + VIH SI +A+
Sbjct: 62 EQGADEI-LAGAGESDVALLVVGDPFGATTHTDFILRAKEKNIPYKVIHNASIMNAVGCC 120
Query: 124 GLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEK----------- 172
GL +YKFG++ +I Y ++ W P S+YD IK N +HTL LDIK ++
Sbjct: 121 GLQLYKFGETVSIPYWDETWKPDSFYDKIKLNRLHNMHTLCLLDIKVKEPTPESLMRKRK 180
Query: 173 ----GKYMTANEGMKLLL----KVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDM 224
++MT E LL K + ++K V +++L V LAR G + I G + +M
Sbjct: 181 EYMPPRFMTVAEAAHQLLSIVEKKDSLEKNTVLNEQSLCVGLARVGQESQQIAVGTLLEM 240
Query: 225 IREDFGSPPHVLIVPGR-LHIVEAEYLVEIA 254
D G P H LI+P + +H +E E+L + A
Sbjct: 241 RSTDLGGPLHSLIIPAKEMHPLEVEFLQQYA 271
>gb|AAF56023.1| (AE003739) Dph5 gene product [Drosophila melanogaster]
Length = 558
Score = 174 bits (436), Expect = 1e-42
Identities = 105/271 (38%), Positives = 153/271 (55%), Gaps = 22/271 (8%)
Query: 4 YFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSREDV 63
Y IGLGL D KDIT+KGLEI ++C V+ E YTS++ G +LE +++ G+ + + R+ V
Sbjct: 280 YLIGLGLGDLKDITVKGLEIVKQCSRVYLEMYTSIL-GCSLEDMQEFYGRPLLLADRDLV 338
Query: 64 ELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAIT 123
E + I L A + DVA L GDP ATTH + LRAK+ + VIH SI +A+
Sbjct: 339 EQGADEI-LAGAGESDVALLVVGDPFGATTHTDFILRAKEKNIPYKVIHNASIMNAVGCC 397
Query: 124 GLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEK----------- 172
GL +YKFG++ +I Y ++ W P S+YD IK N +HTL LDIK ++
Sbjct: 398 GLQLYKFGETVSIPYWDETWKPDSFYDKIKLNRLHNMHTLCLLDIKVKEPTPESLMRKRK 457
Query: 173 ----GKYMTANEGMKLLL----KVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDM 224
++MT E LL K + ++K V +++L V LAR G + I G + +M
Sbjct: 458 EYMPPRFMTVAEAAHQLLSIVEKKDSLEKNTVLNEQSLCVGLARVGQESQQIAVGTLLEM 517
Query: 225 IREDFGSPPHVLIVPGR-LHIVEAEYLVEIA 254
D G P H LI+P + +H +E E+L + A
Sbjct: 518 RSTDLGGPLHSLIIPAKEMHPLEVEFLQQYA 548
>gi|11497993 diphthine synthase (dph5) [Archaeoglobus fulgidus]
>gi|7450868|pir||E69297 diphthine synthase (dph5)
homolog - Archaeoglobus fulgidus
>gi|2650252|gb|AAB90855.1| (AE001078) diphthine synthase
(dph5) [Archaeoglobus fulgidus]
Length = 251
Score = 173 bits (434), Expect = 2e-42
Identities = 105/262 (40%), Positives = 157/262 (59%), Gaps = 17/262 (6%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
L F+GLGL+D KDI++KGLE R+ D V+ E+YTS + +++E +E+ GK + L R D
Sbjct: 2 LTFVGLGLWDVKDISVKGLEAVREADEVYVEYYTSKLL-SSIEEMEEFFGKRVVELERSD 60
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAI-A 121
+E N R++ AK V L GDP+VATTH+ ++L A++ GV++ +IH SI +A+
Sbjct: 61 LEENSFRLI-ERAKSKSVVLLVPGDPMVATTHSAIKLEAERKGVKTRIIHGASISTAVCG 119
Query: 122 ITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTANEG 181
+TGLH Y+FGKSAT+S+ + +VIK N HTLLFLD+ E
Sbjct: 120 LTGLHNYRFGKSATVSWHRSQ----TPVNVIKANRSIDAHTLLFLDLHPEP--------- 166
Query: 182 MKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPGR 241
M + VE++ E ++ V +ARAGS V++ + ++ + DFG P HV++V +
Sbjct: 167 MTIGHAVENLIAEDAQMKDLYAVGIARAGSGEEVVKCDRLENLKKIDFGKPLHVMVVLAK 226
Query: 242 -LHIVEAEYLVEIAGAPREIIR 262
LH +E E L E A AP E+ R
Sbjct: 227 TLHFMEFECLREFADAPAELER 248
>pir||T18768 hypothetical protein B0491.7 - Caenorhabditis elegans
>gi|3873806|emb|CAA90089.1| (Z49907) contains similarity
to Pfam domain: PF00590 (Tetrapyrrole (Corrin/Porphyrin)
Methylases.), Score=82.7, E-value=2.5e-21, N=1~cDNA EST
yk534c1.5 comes from this gene [Caenorhabditis elegans]
Length = 274
Score = 170 bits (427), Expect = 1e-41
Identities = 106/270 (39%), Positives = 143/270 (52%), Gaps = 21/270 (7%)
Query: 1 MALYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMA-GTNLERIEKLIGKEIRVLS 59
M Y IGLGL D +DIT+KGL I + C V E YTS++ G + +EK G+EI
Sbjct: 1 MVFYLIGLGLGDVEDITVKGLNIVKNCARVHLEAYTSILCYGLDKTNLEKFYGREIIEAD 60
Query: 60 REDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSA 119
R VE + I+ K+ DVA L GDP ATTHA+L LRAK+ + VIH SI +A
Sbjct: 61 RTVVEQESDAILNGADKE-DVALLVVGDPFGATTHADLVLRAKQQNIPVKVIHNASIMNA 119
Query: 120 IAITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKA--------- 170
+ GL +Y FG++ +I W P SYYD I N +RG+HTL LDIK
Sbjct: 120 VGCCGLQLYNFGETVSIVMWTDEWQPESYYDKIALNRKRGMHTLCLLDIKTKEQTVENMM 179
Query: 171 ------EKGKYMTANEGMKLLLKVEDMKK----EGVFTQETLVVVLARAGSLNPVIRAGY 220
E +Y +E + LL + + +K E + + T+VV LAR G N I
Sbjct: 180 RGRKIFEPARYQKCSEAARQLLTIYERRKAKGEECAYDENTMVVGLARVGWDNQKIVYAS 239
Query: 221 VRDMIREDFGSPPHVLIVPGRLHIVEAEYL 250
++DM + G P H LI+PG H +E + L
Sbjct: 240 MKDMSEMEMGEPLHSLIIPGETHPLEVDML 269
>gb|AAK41227.1| Diphthine synthase, hypothetical [Sulfolobus solfataricus]
Length = 257
Score = 157 bits (393), Expect = 1e-37
Identities = 93/249 (37%), Positives = 149/249 (59%), Gaps = 6/249 (2%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLS--R 60
L +GLG+ +K IT +E D + + YTS N++ + +L+ E + R
Sbjct: 4 LSLVGLGI-SKKFITDSAIETLSNSDIIIFDRYTSRSCDINVDVLRRLVKGEREFIEADR 62
Query: 61 EDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYS-A 119
+E N + I+ L K ++V+ + GD L+ATTH L + AK G E VI S++
Sbjct: 63 SLLENNSKAIIDYLDKGYNVSIASIGDALIATTHVSLLIEAKHRGHEVKVIPGISVHCYL 122
Query: 120 IAITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTAN 179
I+ + L YKFGKS T+++P ++ + Y+VIK+N ERGLHT+L+LD+K EK MTAN
Sbjct: 123 ISKSLLSSYKFGKSVTVTFPYNDFIDPTPYNVIKDNKERGLHTILYLDLKNEKA--MTAN 180
Query: 180 EGMKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVP 239
E +++LL++E+ K+ V ++ +++V AR G + I A V + DFG+ PH++I+P
Sbjct: 181 EALQILLRLEERHKKSVLSKSDIIIVGARLGCDDERIIALKVEEATSFDFGNTPHIIIIP 240
Query: 240 GRLHIVEAE 248
G LH +EA+
Sbjct: 241 GNLHYMEAD 249
>gb|AAF67485.1|AF157319_1 (AF157319) AD-018 protein [Homo sapiens]
Length = 243
Score = 118 bits (294), Expect = 5e-26
Identities = 88/268 (32%), Positives = 122/268 (44%), Gaps = 73/268 (27%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
LY IGLGL D KDIT+KGLE+ R+C V+ E YTS++ E +E+ G+++ V RE+
Sbjct: 2 LYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLT-VGKEALEEFYGRKLVVADREE 60
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAI 122
VE + I L A DVAFL GD L
Sbjct: 61 VEQEADNI-LKDADISDVAFLVVGDHL--------------------------------- 86
Query: 123 TGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKA------------ 170
W P S++D +K+N + G+HTL LDIK
Sbjct: 87 ------------------DTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGR 128
Query: 171 ---EKGKYMTANEGMKLLLKVEDMKK----EGVFTQETLVVVLARAGSLNPVIRAGYVRD 223
E +YM+ N+ + LL++ ++ E T+ETL V LAR G+ + I AG +R
Sbjct: 129 KIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQ 188
Query: 224 MIREDFGSPPHVLIVP-GRLHIVEAEYL 250
M D G P H LI+ G +H +E E L
Sbjct: 189 MCTVDLGEPLHSLIITGGSIHPMEMEML 216
>gb|AAF29107.1|AF161492_1 (AF161492) HSPC143 [Homo sapiens]
Length = 234
Score = 116 bits (289), Expect = 2e-25
Identities = 89/268 (33%), Positives = 123/268 (45%), Gaps = 73/268 (27%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
LY IGLGL D KDIT+KGLE+ R+C V+ E YTS++ E +E+ G+++ V RE+
Sbjct: 2 LYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLT-VGKEALEEFYGRKLVVADREE 60
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAI 122
VE + I L A DVAFL GDP H E R
Sbjct: 61 VEQEADNI-LKDADISDVAFLVVGDPF---GHLETR------------------------ 92
Query: 123 TGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKA------------ 170
S++D +K+N + G+HTL LDIK
Sbjct: 93 ------------------------SFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGR 128
Query: 171 ---EKGKYMTANEGMKLLLKVEDMKK----EGVFTQETLVVVLARAGSLNPVIRAGYVRD 223
E +YM+ N+ + LL++ ++ E T+ETL V LAR G+ + I AG +R
Sbjct: 129 KIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQ 188
Query: 224 MIREDFGSPPHVLIVP-GRLHIVEAEYL 250
M D G P H LI+ G +H +E E L
Sbjct: 189 MCTVDLGEPLHSLIITGGSIHPMEMEML 216
>emb|CAC12012.1| (AL445065) methyltransferase DPH5 related protein (diphthamide
biosynthesis) [Thermoplasma acidophilum]
Length = 257
Score = 112 bits (277), Expect = 5e-24
Identities = 73/249 (29%), Positives = 131/249 (52%), Gaps = 9/249 (3%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
L IG+GL IT + R D+V+A+ YTS+ + +I +I ++I L+R++
Sbjct: 2 LNIIGVGLRGTGSITFDEFDALRTSDFVYADMYTSIGQPGLIRKISAMIDRDILPLTRDE 61
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIY-SAIA 121
+E +LP A +V+ + GDPL+ATTH ELR A G+ + SI +AI
Sbjct: 62 IE---NGSILPQAASKNVSLIVVGDPLMATTHNELRYEAMNQGIGVRIFENASILNAAIG 118
Query: 122 ITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMTANEG 181
GL +YK ++ + +FP S D IK N + GLHT + +D++ ++ + ++
Sbjct: 119 KAGLMVYKVAPPVSLPRISEKFFPLSVIDKIKRNADLGLHTPVLIDLEDQEN--IPLHDA 176
Query: 182 MKLLLKVEDMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSPPHVLIVPGR 241
+ LL++E ++ +E + VL+R + I G + DM++++ S P+++ + +
Sbjct: 177 LASLLEMERRREYSGIIRE--ICVLSRISFPDEKILFGRIEDMMQQEVNS-PYMMFILSK 233
Query: 242 LHIVEAEYL 250
L E +L
Sbjct: 234 LDDNERRFL 242
>gi|11422835 CGI-30 protein [Homo sapiens]
Length = 349
Score = 106 bits (263), Expect = 2e-22
Identities = 60/123 (48%), Positives = 78/123 (62%), Gaps = 2/123 (1%)
Query: 3 LYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVLSRED 62
LY IGLGL D KDIT+KGLE+ R+C V+ E YTS++ E +E+ G+++ V RE+
Sbjct: 2 LYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLT-VGKEALEEFYGRKLVVADREE 60
Query: 63 VELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVESYVIHAPSIYSAIAI 122
VE + I L A DVAFL GDP ATTH++L LRA K G+ VIH SI +A+
Sbjct: 61 VEQEADNI-LKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGC 119
Query: 123 TGL 125
GL
Sbjct: 120 CGL 122
>pir||C72689 probable diphtine synthase APE0931 - Aeropyrum pernix (strain K1)
>gi|5104600|dbj|BAA79915.1| (AP000060) 294aa long
hypothetical diphtine synthase [Aeropyrum pernix]
Length = 294
Score = 93.2 bits (228), Expect = 3e-18
Identities = 82/268 (30%), Positives = 127/268 (46%), Gaps = 12/268 (4%)
Query: 1 MALYFIGLGLYDEKDITLKGLEIARKCDYVFAEFYTSLMAGTNLERIEKLIGKEIRVL-- 58
+ L +G G Y TL+ L+ R+ D V+ E YT + + + + G E RV+
Sbjct: 8 VTLLLVGWG-YAPGMQTLEALDAVRRADVVYVESYTMPGSSWLYKSVVEAAG-EARVVEA 65
Query: 59 SREDVELNFERIVLPLAKDHDVAFLTAGDPLVATTHAELRLRAKKFGVE-SYVIHAPSIY 117
SR D+E IV A D VA +TAGDP+VATTH+ L A + GV Y+ +
Sbjct: 66 SRRDLEERSREIV-SRALDAVVAVVTAGDPMVATTHSSLAAEALEAGVAVRYIPGVSGVQ 124
Query: 118 SAIAITGLHIYKFGKSATISYPEKNWFPTSYYDVIKENLERGLHTLLFLDIKAEKGKYMT 177
+A T L Y+FG + T+ P + P S I NL GLHT LD+ E+G ++
Sbjct: 125 AARGATMLSFYRFGGTVTLPGPWRGVTPISVARRIYLNLCAGLHTTALLDVD-ERGVQLS 183
Query: 178 ANEGMKLLLKVE-----DMKKEGVFTQETLVVVLARAGSLNPVIRAGYVRDMIREDFGSP 232
+G+ LLL+ + + + + V+V A AG + V+ + + D
Sbjct: 184 PGQGVSLLLEADREYAREAGAPALLARLPSVLVEAGAGGGHRVLYWSSLERLSTADVEGG 243
Query: 233 PHVLIVPGRLHIVEAEYLVEIAGAPREI 260
+ +++P RL VE L +G R +
Sbjct: 244 VYSIVIPARLSGVEEWLLAAASGQRRPL 271
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.141 0.401
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93566274
Number of Sequences: 2977
Number of extensions: 3809489
Number of successful extensions: 8067
Number of sequences better than 1.0e-10: 20
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 8015
Number of HSP's gapped (non-prelim): 23
length of query: 262
length of database: 189,106,746
effective HSP length: 55
effective length of query: 207
effective length of database: 156,189,961
effective search space: 32331321927
effective search space used: 32331321927
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 164 (68.3 bits)