BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1511 (PAB1511) DE:alcohol dehydrogenase, iron-containing
(375 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75040 alcohol dehydrogenase, iron-containing PAB1511 - Pyr... 736 0.0
pir||H71121 probable alcohol dehydrogenase (EC 1.1.1.-) PH0743 [... 651 0.0
sp|P13604|ADH1_CLOAB NADPH-DEPENDENT BUTANOL DEHYDROGENASE (BDH)... 266 2e-70
gb|AAD20601.1| (AF112135) putative NADPH-dependent butanol dehyd... 215 6e-55
sp|Q24803|ADH2_ENTHI ALDEHYDE-ALCOHOL DEHYDROGENASE 2 [INCLUDES:... 208 1e-52
pir||S53319 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.1... 207 2e-52
gi|11497951 alcohol dehydrogenase, iron-containing [Archaeoglobu... 206 4e-52
sp|P17547|ADHE_ECOLI ALDEHYDE-ALCOHOL DEHYDROGENASE [INCLUDES: A... 201 8e-51
gb|AAB51438.1| (U55016) alcohol dehydrogenase [Actinobacillus pl... 198 7e-50
pir||D82127 alcohol dehydrogenase/acetaldehyde dehydrogenase VC2... 196 3e-49
pir||E72498 probable alcohol dehydrogenase APE1963 - Aeropyrum p... 194 1e-48
gb|AAC47539.1| (U93353) alcohol dehydrogenase E [Giardia intesti... 189 6e-47
gb|AAD39016.1| (AF026270) PduQ [Salmonella enterica serovar Typh... 180 2e-44
pir||G69231 1,3-propanediol dehydrogenase - Methanobacterium the... 178 9e-44
sp|P31005|MEDH_BACMT NAD-DEPENDENT METHANOL DEHYDROGENASE (MEDH)... 177 2e-43
emb|CAA33124.1| (X15025) fucO ORF (AA 1-383) [Escherichia coli] 175 6e-43
sp|P11549|FUCO_ECOLI LACTALDEHYDE REDUCTASE (PROPANEDIOL OXIDORE... 175 1e-42
gi|11497645 alcohol dehydrogenase, iron-containing [Archaeoglobu... 174 1e-42
dbj|BAB04253.1| (AP001508) NAD-dependent methanol dehydrogenase ... 173 2e-42
emb|CAA04467.1| (AJ001008) Alcohol-acetaldehyde dehydrogenase [L... 168 7e-41
emb|CAA04465.1| (AJ001007) Alcohol-acetaldehyde dehydrogenase [L... 166 3e-40
sp|P76553|EUTG_ECOLI ETHANOLAMINE UTILIZATION PROTEIN EUTG >gi|1... 165 8e-40
pir||D65020 ethanolamine utilization protein EutG - Escherichia ... 165 8e-40
sp|P37686|ADH2_ECOLI PROBABLE ALCOHOL DEHYDROGENASE >gi|1073456|... 164 1e-39
dbj|BAA75510.1| (AB023641) alcohol dehydrogenase [Rhodospirillum... 164 1e-39
emb|CAA34911.1| (X17065) alcohol dehydrogenase (AA 1-383) [Zymom... 162 7e-39
sp|P06758|ADH2_ZYMMO ALCOHOL DEHYDROGENASE II (ADH II) >gi|78223... 162 7e-39
pir||T33729 alcohol dehydrogenase (EC 1.1.1.1) II - Zymomonas mo... 161 1e-38
gi|11499601 alcohol dehydrogenase, iron-containing [Archaeoglobu... 159 5e-38
sp|Q09669|ADHF_SCHPO PUTATIVE IRON ALCOHOL DEHYDROGENASE >gi|107... 157 2e-37
sp|P33744|ADHE_CLOAB ALDEHYDE-ALCOHOL DEHYDROGENASE [INCLUDES: A... 156 4e-37
pir||A49346 aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) / alcohol... 156 4e-37
emb|CAA29410.1| (X05992) ADH4 (AA 1-382) [Saccharomyces cerevisiae] 153 2e-36
gi|6321181 alcohol dehydrogenase isoenzyme IV; Adh4p [Saccharomy... 153 2e-36
gb|AAC45651.1| (AF006034) 1,3-propanediol dehydrogenase [Clostri... 153 2e-36
gb|AAD43989.1|U59485_16 (U59485) AttL [Agrobacterium tumefaciens] 153 4e-36
pir||A82347 iron-containing alcohol dehydrogenase family protein... 150 2e-35
sp|Q59477|DHAT_KLEPN 1,3-PROPANEDIOL DEHYDROGENASE (3-HYDROXYPRO... 150 3e-35
pir||A72317 alcohol dehydrogenase, iron-containing - Thermotoga ... 149 5e-35
sp|P45513|DHAT_CITFR 1,3-PROPANEDIOL DEHYDROGENASE (3-HYDROXYPRO... 148 1e-34
pir||H82236 iron-containing alcohol dehydrogenase VC1147 [import... 147 2e-34
gb|AAC98741.1| (AF052750) alcohol dehydrogenase [Pseudomonas put... 146 3e-34
pir||G83397 probable iron-containing alcohol dehydrogenase PA199... 145 6e-34
pir||F82998 probable iron-containing alcohol dehydrogenase PA518... 145 8e-34
pir||S28477 probable dehydrogenase (EC 1.1.1.-) rfbM [similarity... 145 1e-33
gb|AAC25557.1| (AF013169) iron containing alcohol dehydrogenase ... 140 3e-32
pir||A83503 probable iron-containing alcohol dehydrogenase PA114... 135 9e-31
emb|CAA74334.1| (Y14015) alcohol dehydrogenase [Thermococcus hyd... 131 1e-29
pir||F72416 alcohol dehydrogenase, iron-containing - Thermotoga ... 127 2e-28
sp|P41795|EUTG_SALTY ETHANOLAMINE UTILIZATION PROTEIN EUTG >gi|6... 127 2e-28
pir||G70398 1,3 propanediol dehydrogenase - Aquifex aeolicus >gi... 127 3e-28
gb|AAC44365.1| (U47861) GbsB [Bacillus subtilis] 126 3e-28
pir||B69629 alcohol dehydrogenase gbsB - Bacillus subtilis >gi|2... 126 3e-28
sp|P71017|GBSB_BACSU ALCOHOL DEHYDROGENASE 126 3e-28
gb|AAB63011.1| (U72646) alcohol dehydrogenase [Thermococcus sp.] 125 6e-28
gb|AAD55928.1|AF148264_1 (AF148264) 4-hydroxybutyrate dehydrogen... 125 6e-28
sp|P38945|4HDB_CLOKL NAD-DEPENDENT 4-HYDROXYBUTYRATE DEHYDROGENA... 123 4e-27
dbj|BAA22024.1| (AB002787) NAD+-dependent alcohol dehydrogenase ... 116 3e-25
emb|CAC11961.1| (AL445065) NADPH-dependent butanol dehydrogenase... 116 5e-25
gb|AAG01186.1|AF178965_1 (AF178965) long-chain primary alcohol d... 114 2e-24
gb|AAG23616.1|AF244667_1 (AF244667) alcohol dehydrogenase [Carbo... 114 2e-24
gb|AAF21473.1|U89273_1 (U89273) methanol:NDMA oxidoreductase [Am... 110 2e-23
pir||I39568 4-hydroxybutyrate dehydrogenase (EC 1.1.1.61) [impor... 108 1e-22
gb|AAB80771.1| (U21071) ThcE [Rhodococcus erythropolis] 107 2e-22
sp|O05239|ADHA_BACSU PROBABLE NADH-DEPENDENT BUTANOL DEHYDROGENA... 101 1e-20
pir||T26686 hypothetical protein Y38F1A.6 - Caenorhabditis elega... 101 1e-20
emb|CAB45024.1| (AL078635) putative alcohol dehydrogenase [Amyco... 100 3e-20
pir||F82428 iron-containing alcohol dehydrogenase VCA0702 [impor... 100 3e-20
pir||S60213 fomC protein - Streptomyces wedmorensis >gi|1061009|... 98 2e-19
sp|O05240|ADHB_BACSU PROBABLE NADH-DEPENDENT BUTANOL DEHYDROGENA... 98 2e-19
pir||G72329 hypothetical protein TM0820 - Thermotoga maritima (s... 98 2e-19
emb|CAC11962.1| (AL445065) NADPH-dependent butanol dehydrogenase... 95 1e-18
pir||B47013 butanol dehydrogenase (EC 1.1.1.-) II - Clostridium ... 94 2e-18
sp|Q04945|ADHB_CLOAB NADH-DEPENDENT BUTANOL DEHYDROGENASE B (BDH... 94 2e-18
gb|AAF46832.1| (AE003457) T3dh gene product [Drosophila melanoga... 93 5e-18
dbj|BAB07063.1| (AP001518) NADH-dependent butanol dehydrogenase ... 90 5e-17
sp|Q04944|ADHA_CLOAB NADH-DEPENDENT BUTANOL DEHYDROGENASE A (BDH... 89 1e-16
gb|AAD19418.1| (AF102543) NADH-dependent butanol dehydrogenase [... 89 1e-16
gb|AAG23601.1|AF244652_1 (AF244652) iron alcohol dehydrogenase [... 89 1e-16
pir||A47013 butanol dehydrogenase (EC 1.1.1.-) I - Clostridium a... 88 1e-16
sp|Q45072|TFTE_BURCE MALEYLACETATE REDUCTASE >gi|2120655|pir||I4... 85 1e-15
gb|AAB07597.1| (U68173) alcohol dehydrogenase [Salmonella typhim... 85 2e-15
sp|P94135|TFF2_ALCEU MALEYLACETATE REDUCTASE II >gi|1747424|gb|A... 85 2e-15
sp|Q24857|ADH3_ENTHI ALCOHOL DEHYDROGENASE 3 (ADH) >gi|1381024|d... 84 2e-15
sp|Q46856|YQHD_ECOLI HYPOTHETICAL OXIDOREDUCTASE IN METC-SUFI IN... 83 6e-15
sp|P27137|TFF1_ALCEU MALEYLACETATE REDUCTASE I >gi|95490|pir||D3... 81 2e-14
sp|O84992|MCA1_RHOOP MALEYLACETATE REDUCTASE I >gi|3387795|gb|AA... 78 2e-13
gb|AAK42626.1| Maleylacetate reductase (clcE) [Sulfolobus solfat... 76 8e-13
gb|AAD55886.1|AF130250_3 (AF130250) maleylacetate reductase [Ral... 76 8e-13
pir||S68143 alcohol dehydrogenase (acceptor) (EC 1.1.99.8) - Ent... 74 2e-12
gb|AAF45302.1|AF239724_1 (AF239724) alcohol dehydrogenase [Giard... 73 5e-12
gb|AAF59422.1| (U32188) putative chloromaleylacetate reductase [... 72 1e-11
>pir||C75040 alcohol dehydrogenase, iron-containing PAB1511 - Pyrococcus abyssi
(strain Orsay) >gi|5458729|emb|CAB50216.1| (AJ248287)
alcohol dehydrogenase, iron-containing [Pyrococcus
abyssi]
Length = 375
Score = 736 bits (1879), Expect = 0.0
Identities = 375/375 (100%), Positives = 375/375 (100%)
Query: 1 MRFFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
MRFFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI
Sbjct: 1 MRFFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS
Sbjct: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLP 180
RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLP
Sbjct: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLP 180
Query: 181 EKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREE 240
EKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREE
Sbjct: 181 EKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREE 240
Query: 241 VHYAATMAGIAFLNGRLGLVHAMSHKSAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMA 300
VHYAATMAGIAFLNGRLGLVHAMSHKSAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMA
Sbjct: 241 VHYAATMAGIAFLNGRLGLVHAMSHKSAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMA 300
Query: 301 RELGLSSADELLEKVKELNEKLGVPKLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEP 360
RELGLSSADELLEKVKELNEKLGVPKLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEP
Sbjct: 301 RELGLSSADELLEKVKELNEKLGVPKLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEP 360
Query: 361 SVEDIKNLYLRALHD 375
SVEDIKNLYLRALHD
Sbjct: 361 SVEDIKNLYLRALHD 375
>pir||H71121 probable alcohol dehydrogenase (EC 1.1.1.-) PH0743 [similarity] -
Pyrococcus horikoshii >gi|3257151|dbj|BAA29834.1|
(AP000003) 375aa long hypothetical alchol dehydrogenase
[Pyrococcus horikoshii]
Length = 375
Score = 651 bits (1660), Expect = 0.0
Identities = 316/375 (84%), Positives = 354/375 (94%)
Query: 1 MRFFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
M FFLKTTILMGRGSLEN+K+++SEGERVL+FSS+SMDRLGFL EV DY++EAG YE+I
Sbjct: 1 MTFFLKTTILMGRGSLENIKKLVSEGERVLVFSSKSMDRLGFLKEVIDYLDEAGATYESI 60
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
GLP EPS+ENVEE+LPKV++FSPETFIALGGGSVID++KA+KVFYDAP LDFDSVAIFS
Sbjct: 61 TGLPSEPSIENVEELLPKVKDFSPETFIALGGGSVIDISKALKVFYDAPELDFDSVAIFS 120
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLP 180
RFKKAQPLPKLKT+LIA+PSTSGAGSEVSAA+VIKKGD KYTIVSPELCP+YAILDPRLP
Sbjct: 121 RFKKAQPLPKLKTKLIAVPSTSGAGSEVSAATVIKKGDIKYTIVSPELCPNYAILDPRLP 180
Query: 181 EKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREE 240
E MPREVARNSGLDVLVHAIEAYVSKASTPFSDA+AVKAAR I E+LEDSVNGD AREE
Sbjct: 181 ENMPREVARNSGLDVLVHAIEAYVSKASTPFSDAMAVKAARTILEKLEDSVNGDPTAREE 240
Query: 241 VHYAATMAGIAFLNGRLGLVHAMSHKSAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMA 300
VHYAATMAGIAFLNGRLGLVHAMSHK+AWIGPHGL+NAI LPYVMEFNME+AREKYDAMA
Sbjct: 241 VHYAATMAGIAFLNGRLGLVHAMSHKAAWIGPHGLINAILLPYVMEFNMEKAREKYDAMA 300
Query: 301 RELGLSSADELLEKVKELNEKLGVPKLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEP 360
+ELGLS+A+ELL+KVKELNE+L VPKLSEIV EE+F ++++MSRK YEDPLV+FNPVEP
Sbjct: 301 KELGLSNAEELLQKVKELNERLNVPKLSEIVSEEDFTSRLDEMSRKAYEDPLVNFNPVEP 360
Query: 361 SVEDIKNLYLRALHD 375
SV+DIKN+YLRA HD
Sbjct: 361 SVDDIKNIYLRAFHD 375
>sp|P13604|ADH1_CLOAB NADPH-DEPENDENT BUTANOL DEHYDROGENASE (BDH) >gi|80486|pir||JU0053
NADPH-dependent butanol dehydrogenase (EC 1.1.1.-) -
Clostridium acetobutylicum >gi|144714|gb|AAA83520.1|
(M26941) NADPH-dependent butanol dehydrogenase
[Clostridium acetobutylicum]
Length = 388
Score = 266 bits (674), Expect = 2e-70
Identities = 152/383 (39%), Positives = 234/383 (60%), Gaps = 21/383 (5%)
Query: 1 MRFFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
MRF L I G+GSLE +K + + + +L+ SM R GF+ +V Y++EAG + I
Sbjct: 2 MRFTLPRDIYYGKGSLEQLKNLKGK-KAMLVLGGGSMKRFGFVDKVLGYLKEAGIEVKLI 60
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ P+PSVE V + + +F P+ IA+GGGS ID AKA+ +FY+ P FD +
Sbjct: 61 EGVEPDPSVETVFKGAELMRQFEPDWIIAMGGGSPIDAAKAMWIFYEHPEKTFDDI---- 116
Query: 121 RFKKAQPLPKL--KTRLIAIPSTSGAGSEVSAASVIK--KGDTKYTIVSPELCPDYAILD 176
K +P+L K + +AIPSTSG +EV+A SVI K + KY + + PD A++D
Sbjct: 117 --KDPFTVPELRNKAKFLAIPSTSGTATEVTAFSVITDYKTEIKYPLADFNITPDVAVVD 174
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAK 236
L E MP ++ ++G+D L HAIEAYV+ +PF+D LA++A +I E L S GD +
Sbjct: 175 SELAETMPPKLTAHTGMDALTHAIEAYVATLHSPFTDPLAMQAIEMINEHLFKSYEGDKE 234
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG--PHGLVNAIFLPYVMEFNMERARE 294
ARE++HYA +AG+AF N LG+ H+M+HK+ + PHG NAI+LPYV++FN + + E
Sbjct: 235 AREQMHYAQCLAGMAFSNALLGICHSMAHKTGAVFHIPHGCANAIYLPYVIKFNSKTSLE 294
Query: 295 KYDAMARELGLSS------ADELLEKVKELNEKLGVP-KLSEI-VKEEEFLGKVEDMSRK 346
+Y +A+++ L+ D L+ VKELN+K+ +P L E + E+EF KV+ +S +
Sbjct: 295 RYAKIAKQISLAGNTNEELVDSLINLVKELNKKMQIPTTLKEYGIHEQEFKNKVDLISER 354
Query: 347 TYEDPLVSFNPVEPSVEDIKNLY 369
D NP + + +++K ++
Sbjct: 355 AIGDACTGSNPRQLNKDEMKKIF 377
>gb|AAD20601.1| (AF112135) putative NADPH-dependent butanol dehydrogenase
[Trichomonas vaginalis]
Length = 396
Score = 215 bits (542), Expect = 6e-55
Identities = 139/396 (35%), Positives = 219/396 (55%), Gaps = 29/396 (7%)
Query: 2 RFFLKTTILMGRGSLENVKRIISEGERVLIFS-SRSMDRLGFLSEVADYVEEAGGVYETI 60
RF L + G+G+LEN+K + +G+R + + S +M + GFL +V +Y++EA
Sbjct: 3 RFTLPRVLYHGKGALENLKTL--KGKRAFVVTGSHAMRKFGFLDKVVNYLKEAKFEVHVF 60
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ +PSVE V + +EEF P+ +ALGGGS ID AKA+ Y+ P + F+ + +
Sbjct: 61 DGVEQDPSVETVLKGAKTMEEFKPDWIVALGGGSPIDAAKAMWAVYEYPEMTFEKLIVPF 120
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI---KKGDTKYTIVSPELCPDYAILDP 177
F P + K AI STSG +EV+A SVI +KG KY + + PD AI+DP
Sbjct: 121 SF----PELRQKAHFCAITSTSGTATEVTAFSVITDYQKG-IKYPLADFNITPDVAIVDP 175
Query: 178 RLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKA 237
L E MP+++ +G+D L HA EAYVS ++D LA+ + ++IFE LE S NG KA
Sbjct: 176 DLAETMPKKLVAFTGMDALTHAFEAYVSTLHCDYTDGLALHSIKLIFETLEASFNGCMKA 235
Query: 238 REEVHYAATMAGIAFLNG---RLGLVHAMSHKSAWIGPH--GLVNAIFLPYVMEFNM--E 290
R +H A +AG+AF N L + PH L NA++LP V+++N E
Sbjct: 236 RATMHNAQCLAGMAFSNSPPRHCPLNGPQDRRCLHKWPHRPRLRNAMYLPKVIKYNSKNE 295
Query: 291 RAREKYDAMARELGLSSADE------LLEKVKELNEKLGVPKL-----SEIVKEEEFLGK 339
A +Y +AR LG+ D+ + +++++L K+ VP + ++ E+E+L K
Sbjct: 296 EALHRYGDIARFLGMQGKDDVELVDAICQRIRDLCVKIEVPLCIKDYENGMINEQEYLAK 355
Query: 340 VEDMSRKTYEDPLVSFNPVEPSVEDIKNLYLRALHD 375
++D++ D NP +P+ E+++ L L +D
Sbjct: 356 LKDVATNAIGDACTGSNPRQPTQEEMEKLLLAVFYD 391
>sp|Q24803|ADH2_ENTHI ALDEHYDE-ALCOHOL DEHYDROGENASE 2 [INCLUDES: ALCOHOL DEHYDROGENASE
(ADH); ALCETALDEHYDE DEHYDROGENASE (ACDH)]
>gi|488430|gb|AAA81906.1| (U04863) alcohol dehydrogenase
2 [Entamoeba histolytica]
Length = 870
Score = 208 bits (523), Expect = 1e-52
Identities = 133/388 (34%), Positives = 211/388 (54%), Gaps = 40/388 (10%)
Query: 23 ISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYET--IIGLPPEPSVENVEEVLPKVE 80
+ E ++ I S R M +LG++ V D ++ E I + P+PS++ V++ L +
Sbjct: 484 LKELSKIFIVSDRMMYKLGYVDRVMDVLKRRSNEVEIEIFIDVEPDPSIQTVQKGLAVMN 543
Query: 81 EFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRF----KKAQPLPKL--KTR 134
F P+ IA+GGGS +D AK + + Y+ P DF A+ +F K+A P + K R
Sbjct: 544 TFGPDNIIAIGGGSAMDAAKIMWLLYEHPEADF--FAMKQKFIDLRKRAFKFPTMGKKAR 601
Query: 135 LIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPEKMPREVARNSG 192
LI IP+TSG GSEV+ +VI +T KY + L P AI+DP +P+ ++G
Sbjct: 602 LICIPTTSGTGSEVTPFAVISDHETGKKYPLADYSLTPSVAIVDPMFTMSLPKRAIADTG 661
Query: 193 LDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREEVHYAATMAGIAF 252
LDVLVHA EAYVS + ++D LA +A +++FE L S NGD +ARE++H AAT+AG+AF
Sbjct: 662 LDVLVHATEAYVSVMANEYTDGLAREAVKLVFENLLKSYNGDLEAREKMHNAATIAGMAF 721
Query: 253 LNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAR--------------EKY 296
+ LG+ H+M+HK +A+ PHG A+ LP+V+ +N ++ R ++Y
Sbjct: 722 ASAFLGMDHSMAHKVGAAFHLPHGRCVAVLLPHVIRYNGQKPRKLAMWPKYNFYKADQRY 781
Query: 297 DAMARELGL----------SSADELLEKVKELNEKLGVPKLSEIVKEEEFLGKVEDMSRK 346
+A+ +GL + A E +K G K + + E ++ KV +M+
Sbjct: 782 MELAQMVGLKCNTPAEGVEAFAKACEELMKATETITGFKKAN--IDEAAWMSKVPEMALL 839
Query: 347 TYEDPLVSFNPVEPSVEDIKNLYLRALH 374
+ED NP P V+D++ + A +
Sbjct: 840 AFEDQCSPANPRVPMVKDMEKILKAAYY 867
>pir||S53319 acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10) / alcohol
dehydrogenase (EC 1.1.1.1) - Entamoeba histolytica
>gi|443985|emb|CAA54388.1| (X77132) alcohol
dehydrogenase [Entamoeba histolytica]
Length = 872
Score = 207 bits (521), Expect = 2e-52
Identities = 134/390 (34%), Positives = 216/390 (55%), Gaps = 40/390 (10%)
Query: 21 RIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYET--IIGLPPEPSVENVEEVLPK 78
R + E ++ I S R M +LG++ V D ++ E I + P+PS++ V++ L
Sbjct: 484 RELKELSKIFIVSDRMMYKLGYVDRVMDVLKRRSNEVEIEIFIDVEPDPSIQIVQKGLAV 543
Query: 79 VEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRF----KKAQPLPKL--K 132
+ F P+ IA+GGGS +D AK +++ Y+ P DF A+ +F K+A P + K
Sbjct: 544 MNTFGPDNIIAIGGGSAMDAAKIMRLLYEHPEADF--FAMKQKFIDLRKRAFKFPTMGKK 601
Query: 133 TRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPEKMPREVARN 190
RLI IP+TSG GSEV+ +VI +T KY + L P AI+DP +P+ +
Sbjct: 602 ARLICIPTTSGTGSEVTPFAVISDHETGKKYPLADYSLTPSVAIVDPMFTMSLPKRAIAD 661
Query: 191 SGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREEVHYAATMAGI 250
+GLDVLVHA EAYVS + ++D LA +A +++FE L S NGD +ARE++H AAT+AG+
Sbjct: 662 TGLDVLVHATEAYVSVMANEYTDGLAREAVKLVFENLLKSYNGDLEAREKMHNAATIAGM 721
Query: 251 AFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAR--------------E 294
AF + LG+ H+M+HK + + PHG A+ LP+V+ +N ++ R +
Sbjct: 722 AFASAFLGMDHSMAHKVGAGFHLPHGRCVAVLLPHVIRYNGQKPRKLAMWPKYNFYKADQ 781
Query: 295 KYDAMARELGL--SSADELLEKVKELNEKLGVPKLSEI--------VKEEEFLGKVEDMS 344
+Y +A+ +GL ++ E +E + E+L K +E + E ++ KV +M+
Sbjct: 782 RYMELAQMVGLKCNTPAEGVEAFAKACEEL--MKATETITGFKQANIDEAAWMSKVPEMA 839
Query: 345 RKTYEDPLVSFNPVEPSVEDIKNLYLRALH 374
+ED NP P V+D++ + A +
Sbjct: 840 LLAFEDQCSPANPRVPMVKDMEKILKAAYY 869
>gi|11497951 alcohol dehydrogenase, iron-containing [Archaeoglobus fulgidus]
>gi|7431293|pir||C69292 alcohol dehydrogenase,
iron-containing homolog - Archaeoglobus fulgidus
>gi|2650296|gb|AAB90896.1| (AE001081) alcohol
dehydrogenase, iron-containing [Archaeoglobus fulgidus]
Length = 395
Score = 206 bits (518), Expect = 4e-52
Identities = 130/375 (34%), Positives = 207/375 (54%), Gaps = 14/375 (3%)
Query: 1 MRFFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
M +F+ + G ++E+++ ERVLI + + + + +L EV ++ E
Sbjct: 16 MWWFVVPKTVFGDDAIEHLQS--ERAERVLIVTDKVLSEMSYLEEVKKNLKAER--IEVF 71
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
+ PEPS+E + E + IALGGGSV+DVAK ++ + LD D I
Sbjct: 72 DEVEPEPSIETALKCSKVARELDAQLIIALGGGSVMDVAKMARILME---LDVDPEFITP 128
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIV--SPELCPDYAILDPR 178
+ + K +LIAIP+TSG G++ + A V+ K ++ + E PD ILD R
Sbjct: 129 FTDLYELGFQKKAKLIAIPTTSGTGADATWAMVLTDTKEKRKVLPANREAIPDLTILDYR 188
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAR 238
E +P ++ +G+D L HAIE ++ FSDA+ KA +IF+ LE S NGD +AR
Sbjct: 189 FVENLPPKLVAGTGMDALTHAIEGALAPWRNDFSDAMCEKAIELIFDNLEKSYNGDKEAR 248
Query: 239 EEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEF--NMERARE 294
++H+AATMAG+ F N ++G VH++ H + + HG+ A+FLPYV++F N E +R
Sbjct: 249 AKMHHAATMAGMGFGNSQVGPVHSLGHAFGAYFKVHHGICVAVFLPYVLQFYLNDEGSRA 308
Query: 295 KYDAMARELGLSSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKTYEDPLV 353
D +A +GL S + ++EKV +L +K+ P K+S+IV E+E+ G +ED+ D +
Sbjct: 309 MMDYLAGRVGLESGESMVEKVMDLMKKIEAPTKVSDIVGEKEYFGAIEDIWSHAINDACM 368
Query: 354 SFNPVEPSVEDIKNL 368
+P P E + L
Sbjct: 369 ITSPRCPDTEQTRKL 383
>sp|P17547|ADHE_ECOLI ALDEHYDE-ALCOHOL DEHYDROGENASE [INCLUDES: ALCOHOL DEHYDROGENASE
(ADH); ACETALDEHYDE DEHYDROGENASE [ACETYLATING] (ACDH);
PYRUVATE-FORMATE-LYASE DEACTIVASE (PFL DEACTIVASE)]
>gi|65948|pir||DEEC acetaldehyde dehydrogenase
(acetylating) (EC 1.2.1.10) / alcohol dehydrogenase (EC
1.1.1.1) - Escherichia coli >gi|40900|emb|CAA41955.1|
(X59263) alcohol dehydrogenase [Escherichia coli]
>gi|145206|gb|AAA23420.1| (M33504) alcohol dehydrogenase
(adhE) [Escherichia coli] >gi|1651639|dbj|BAA36121.1|
(D90759) Alcohol dehydrogenase (EC 1.1.1.1).
[Escherichia coli] >gi|1787493|gb|AAC74323.1| (AE000222)
CoA-linked acetaldehyde dehydrogenase and iron-dependent
alcohol dehydrogenase; pyruvate-formate-lyase deactivase
[Escherichia coli K12] >gi|1805517|dbj|BAA16034.1|
(D90852) alcohol dehydrogenase (EC 1.1.1.1) [Escherichia
coli] >gi|4867938|dbj|BAA77747.1| (AB008676) alcohol
dehydrogenase [Escherichia coli]
Length = 891
Score = 201 bits (507), Expect = 8e-51
Identities = 139/406 (34%), Positives = 218/406 (53%), Gaps = 42/406 (10%)
Query: 5 LKTTILMGRGSLE-NVKRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIG 62
L +I RGSL + +I++G +R LI + R + G+ ++ ++ AG E
Sbjct: 456 LPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGVETEVFFE 515
Query: 63 LPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRF 122
+ +P++ V + F P+ IALGGGS +D AK + V Y+ P F+ +A+ RF
Sbjct: 516 VEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHFEELAL--RF 573
Query: 123 ----KKAQPLPKL--KTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAI 174
K+ PK+ K ++IA+ +TSG GSEV+ +V+ T KY + L PD AI
Sbjct: 574 MDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLADYALTPDMAI 633
Query: 175 LDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGD 234
+D L MP+ + GLD + HA+EAYVS ++ FSD A++A +++ E L S +
Sbjct: 634 VDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLLKEYLPASYHEG 693
Query: 235 AK---AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNM 289
+K ARE VH AAT+AGIAF N LG+ H+M+HK S + PHGL NA+ + V+ +N
Sbjct: 694 SKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLANALLICNVIRYNA 753
Query: 290 E---------------RAREKYDAMARELGLSS--------ADELLEKVKELNEKLGVPK 326
+AR +Y +A LGLS+ ++LL ++ L +LG+PK
Sbjct: 754 NDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKLLAWLETLKAELGIPK 813
Query: 327 -LSEI-VKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYL 370
+ E V+E +FL V+ +S ++D NP P + ++K + L
Sbjct: 814 SIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQILL 859
>gb|AAB51438.1| (U55016) alcohol dehydrogenase [Actinobacillus pleuropneumoniae]
Length = 368
Score = 198 bits (499), Expect = 7e-50
Identities = 134/367 (36%), Positives = 203/367 (54%), Gaps = 34/367 (9%)
Query: 40 LGFLSEVADYVEEAGG--VYETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVID 97
LGF+ + D + + + + + P PS++ V+ + F P+T IALGGGS +D
Sbjct: 2 LGFVDRITDQLRQRRNKVMIQLFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMD 61
Query: 98 VAKAVKVFYDAPGLDF-DSVAIFSRFKK-AQPLPKL--KTRLIAIPSTSGAGSEVSAASV 153
AK + +FY+ P +DF D V F +K A P+L K + + IP+TSG + SA V
Sbjct: 62 AAKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTVPK-SAVCV 120
Query: 154 IKKGDTKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSD 213
I GD KY + L P AI+DP L +P VA ++GLDVL HA EAY S + ++D
Sbjct: 121 ITDGDIKYPLADYSLTPTIAIVDPALVMSVPAHVAADTGLDVLTHATEAYTSILANDYTD 180
Query: 214 ALAVKAARIIFERLEDSVNG-DAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWI 270
LA++A +++FE LE SV D ARE++H A+TMAGIAF N LG+ H+M+HK +
Sbjct: 181 GLALQAIKLVFENLEKSVKEFDEVAREKMHNASTMAGIAFANAFLGICHSMAHKIGGKFH 240
Query: 271 GPHGLVNAIFLPYVMEFNMER--------------AREKYDAMARELGL--SSADELLEK 314
HG NAI LP+V+ +N R A +++ +AR LGL ++ +E +E
Sbjct: 241 TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADKRFQDIARILGLPATTPEEAVES 300
Query: 315 ----VKELNEKLGVPKLS---EIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKN 367
V +L + GV K+S + + E+EFL +++ +ED NP VED++
Sbjct: 301 YAKAVHDLAVRCGV-KMSLREQGIDEQEFLDARRELALNAFEDQCTPANPRLAMVEDMEE 359
Query: 368 LYLRALH 374
+ +A +
Sbjct: 360 IMTKAYY 366
>pir||D82127 alcohol dehydrogenase/acetaldehyde dehydrogenase VC2033 [imported]
- Vibrio cholerae (group O1 strain N16961)
>gi|9656578|gb|AAF95181.1| (AE004277) alcohol
dehydrogenase/acetaldehyde dehydrogenase [Vibrio
cholerae]
Length = 894
Score = 196 bits (494), Expect = 3e-49
Identities = 132/408 (32%), Positives = 210/408 (51%), Gaps = 40/408 (9%)
Query: 5 LKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLP 64
L +I RGSL + +R + + R + G+ +V ++ G +T +
Sbjct: 457 LPKSIYFRRGSLPIALSDLEGKKRAFLVTDRFLFNNGYADDVVALLKAQGMEVQTFFEVE 516
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRF-- 122
+P++ VE+ ++ F P+ +ALGGGS +D AK + V Y+ P F+ +A+ RF
Sbjct: 517 ADPTLSVVEKGAAAMQSFQPDVILALGGGSPMDAAKIMWVMYEHPDTHFEELAM--RFMD 574
Query: 123 --KKAQPLPKL--KTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILD 176
K+ PK+ K L+ I +TSG GSEV+ +V+ T KY + EL P AI+D
Sbjct: 575 IRKRIYKFPKMGKKAELVCITTTSGTGSEVTPFAVVTDDKTGAKYPLADYELTPQMAIVD 634
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAK 236
L MP+ + G D + HA+EAYVS + +SD A++A +++ E L S AK
Sbjct: 635 ANLVMNMPKSLTAFGGYDAVTHALEAYVSVLANEYSDGQALQALKMLKEYLPSSYANGAK 694
Query: 237 ---AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNME- 290
ARE+VH AAT+AGIAF N LG+ H+M+HK + + PHGL NA+ + V+ +N
Sbjct: 695 DPIAREKVHNAATIAGIAFANAFLGVCHSMAHKIGAEFHLPHGLANALLIANVVRYNAND 754
Query: 291 --------------RAREKYDAMARELGLS--------SADELLEKVKELNEKLGVPKLS 328
+AR +Y +A LGLS + LL + EL L +PK
Sbjct: 755 NPTKQTAFSQYDRPQARRRYAEVADHLGLSQPGDRTAQKIERLLTWLDELKVNLDIPKSI 814
Query: 329 EI--VKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYLRALH 374
+ V E +FL KV++++ + ++D NP P + ++K + L + +
Sbjct: 815 QAAGVAEADFLAKVDELAVEAFDDQCTGANPRYPLIAELKEVLLASYY 862
>pir||E72498 probable alcohol dehydrogenase APE1963 - Aeropyrum pernix (strain
K1) >gi|5105661|dbj|BAA80973.1| (AP000063) 399aa long
hypothetical alcohol dehydrogenase [Aeropyrum pernix]
Length = 399
Score = 194 bits (488), Expect = 1e-48
Identities = 126/360 (35%), Positives = 193/360 (53%), Gaps = 22/360 (6%)
Query: 27 ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPET 86
+ VL+ + + + + E ++V G G+ PEP + + + + E
Sbjct: 39 KHVLVVTDGKVAAMSWFKEAVEHVASLGVEVCIFDGVTPEPEFDVGDAIASEARRCRAEA 98
Query: 87 FIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGS 146
+A+GGGSVID AKA V + P D + +A F+ L L+A+P+TSG GS
Sbjct: 99 IVAVGGGSVIDAAKAGFVKFVRPDADLEGLAPFNYLG----LEDSGIVLVAVPTTSGTGS 154
Query: 147 EVSAASVIKK----GDTKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEA 202
+ S V+ K G K + S E+ P +ILDP + MP + + +D L H++EA
Sbjct: 155 DASYGIVLTKRVNGGREKIAVGSYEVVPYASILDPSITLGMPERLTVGTAVDALAHSVEA 214
Query: 203 YVSKASTPFSDALAVKAARIIFERLEDSVN--GDAKAREEVHYAATMAGIAFLNGRLGLV 260
S + P SDALA KAA I+F RL + VN G+ AR E+H AATMAG+AF N LGL
Sbjct: 215 IASTNANPLSDALAAKAAEIVFRRLPEVVNDPGNYDARAEMHIAATMAGMAFTNSGLGLA 274
Query: 261 HAMSHK-SAWIGP-HGLVNAIFLPYVMEFNMER---AREKY----DAMARELGLSSADEL 311
HA++H A +G HG + LP+V+ FN R AR KY + LGL L
Sbjct: 275 HAIAHPLGARLGTHHGATVGMVLPHVVRFNESRSDYARRKYWELKLLLESLLGLEERGSL 334
Query: 312 LEKVKELNEKLGVP-KLSEI--VKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNL 368
+ ++EL ++G P ++ E+ ++ E +L ++++ + DP ++FNPV P++ED+K L
Sbjct: 335 ADHIEELYARVGQPTRVRELAGIERERYLTLADEVAEAAFRDPELAFNPVMPTMEDVKTL 394
>gb|AAC47539.1| (U93353) alcohol dehydrogenase E [Giardia intestinalis]
Length = 888
Score = 189 bits (474), Expect = 6e-47
Identities = 133/408 (32%), Positives = 210/408 (50%), Gaps = 46/408 (11%)
Query: 11 MGRGSLENVKR-IISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPS 68
+G G+L +IS G R I + + M LGF ++ + + + P+P
Sbjct: 480 VGEGALAKAAADLISRGLSRAYIITGKVMHDLGFTDKIISPLTAGNVTVKVFTDVLPDPD 539
Query: 69 VENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAI--------FS 120
+ L +V +F P+ IALGGGS +D+AK V++ Y+ P +DF +A
Sbjct: 540 LGTCYRSLAEVRDFQPDMIIALGGGSAMDLAKMVRLLYEHPKVDFAGLAQRFMDIRKRIY 599
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
+ + L KT +AIP+TSG GSEV+ SVI +K KY + +L A++DP
Sbjct: 600 EYPECLDLRTAKTFSVAIPTTSGTGSEVTPFSVITDEKEHVKYPLADYQLMTHMAVIDPE 659
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDS-VNGDAKA 237
L +P +A +G+D L HAIE+YVS +T ++ L+++A + +FE LE S V+ A
Sbjct: 660 LVLTVPASLASWTGVDALTHAIESYVSVMATEYTMPLSLQAIKTVFENLEKSVVSRCPTA 719
Query: 238 REEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFN------- 288
R VH AAT+AGIAF N LG+ H+ +HK + PHGL NAI LP+V+ +N
Sbjct: 720 RGNVHQAATIAGIAFANAFLGICHSCAHKLGQKYHIPHGLANAIMLPHVIRYNAVDDPVK 779
Query: 289 --------MERAREKYDAMARELGLSSADE----------LLEKVKELNEKLGV-PKLS- 328
A E+Y +A G ++ ++ L++K+ +L EK+G+ K+S
Sbjct: 780 IATFPQYLYPVALERYAEIADYCGFTNKNDGKSVKEKTEILIKKIYDLYEKVGIDAKISA 839
Query: 329 --EIVKEEEFLGK--VEDMSRKTYEDPLVSFNPVEPSVEDIKNLYLRA 372
E E +F + ++ ++ ++D NP P +ED K L+ A
Sbjct: 840 CKEAPVEADFFAEENLDYLAYHAFDDQCTGANPRYPLIEDFKELFRAA 887
>gb|AAD39016.1| (AF026270) PduQ [Salmonella enterica serovar Typhimurium]
Length = 370
Score = 180 bits (453), Expect = 2e-44
Identities = 121/384 (31%), Positives = 206/384 (53%), Gaps = 40/384 (10%)
Query: 3 FFLKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIG 62
F L+T + G+GSL +KR ++ + I + L + + + A
Sbjct: 4 FSLQTRLYSGQGSLAVLKRFTNK--HIWIICDGFLAHSPLLDTLRNALP-ADNRISVFSE 60
Query: 63 LPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRF 122
+ P+P++ V + + +++ P+ I GGGS +D AKA+ F G++ ++
Sbjct: 61 ITPDPTIHTVVQGIAQMQALQPQVVIGFGGGSAMDAAKAIVWFSQQSGINIETC------ 114
Query: 123 KKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLP 180
+AIP+TSG GSEV++A VI D KY + + L PD AILDP L
Sbjct: 115 -------------VAIPTTSGTGSEVTSACVISDPDKGIKYPLFNNALYPDMAILDPELV 161
Query: 181 EKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV-NGDAKA-R 238
+P ++ N+G+DVL HA+EA+VS ++ F+DALA KAA+++F+ L +V GD A R
Sbjct: 162 VSVPPQITANTGMDVLTHALEAWVSPRASDFTDALAEKAAKLVFQYLPTAVEKGDCVATR 221
Query: 239 EEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFN--MERARE 294
++H A+T+AG+AF LGL HA++H+ + PHGL NA+ L V+ FN + RA +
Sbjct: 222 GKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTTVIRFNAGVPRAAK 281
Query: 295 KYDAMARELG--------LSSADELLEKVKELNEKLGVPKLSEIVKE--EEFLGKVEDMS 344
+Y +A+ G +++ + L+++++ L ++ +P L+ +KE +F ++ M
Sbjct: 282 RYARLAKACGFCPAEANDIAAINALIQQIELLKQRCVLPSLAVALKEGRSDFSARIPAMV 341
Query: 345 RKTYEDPLVSFNPVEPSVEDIKNL 368
+ D + NP + E I+ L
Sbjct: 342 QAALADVTLRTNPRPANAEAIREL 365
>pir||G69231 1,3-propanediol dehydrogenase - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622085|gb|AAB85481.1| (AE000872) 1,3-propanediol
dehydrogenase [Methanobacterium thermoautotrophicum]
Length = 341
Score = 178 bits (447), Expect = 9e-44
Identities = 118/351 (33%), Positives = 185/351 (52%), Gaps = 21/351 (5%)
Query: 33 SSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGG 92
+ R + + GF+ +V+ +EE G Y + P P V E + +ALGG
Sbjct: 2 TDRGLMKTGFVEDVSSSLEEEGLEYVIFDDVTPNPRDHEVMEGAEVFDAEECNMIVALGG 61
Query: 93 GSVIDVAKAVKVFYD--APGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSA 150
GS ID AKA+ LDF+ V + +P +I IP+T+G G++VS
Sbjct: 62 GSPIDCAKAMGAVVSNRMHVLDFEGVDM---------IPTPSVPIICIPTTAGTGADVSQ 112
Query: 151 ASVI--KKGDTKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKAS 208
++I + K I+S + PD +++DP M RE+ ++G+D L H+IEAYVS AS
Sbjct: 113 FAIILDTRRMVKIAIISKAMIPDASLIDPETTVTMDRELTASTGMDALAHSIEAYVSNAS 172
Query: 209 TPFSDALAVKAARIIFERLEDSVN--GDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK 266
+D A+ A RII L +V+ + + RE + A+ AG+AF N LGLVHAM+H
Sbjct: 173 FHLTDLNALDAIRIISRCLPSAVDEPENMEYREGMMLASLEAGLAFSNASLGLVHAMAHS 232
Query: 267 --SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELGL--SSADELLEKVKELNEKL 322
HG NA+ L +V+EFN A E+Y +AR +GL S EL++ ++E +
Sbjct: 233 LGGKLDMAHGEANALLLEHVIEFNYPGATERYHEIARAMGLRGSGPAELIDYIREFRSSI 292
Query: 323 GVPKLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYLRAL 373
G+ E + E + +++ + +DP + NPV PS+ED++ ++ RAL
Sbjct: 293 GIDMTLEDLGVGE--DDIPELAEASMKDPCIVTNPVRPSIEDVEAIFRRAL 341
>sp|P31005|MEDH_BACMT NAD-DEPENDENT METHANOL DEHYDROGENASE (MEDH) >gi|285326|pir||A42952
methanol dehydrogenase (EC 1.1.1.244) - Bacillus sp
>gi|143175|gb|AAA22593.1| (M65004) methanol
dehydrogenase alpha-10 subunit [Bacillus sp.]
Length = 381
Score = 177 bits (444), Expect = 2e-43
Identities = 123/389 (31%), Positives = 207/389 (52%), Gaps = 29/389 (7%)
Query: 3 FFLKTTILMGRGSLENVKRIISE--GERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
FF+ ++GRG+++ V + + ++ LI + + G EVA + EAG
Sbjct: 4 FFIPPASVIGRGAVKEVGTRLKQIGAKKALIVTDAFLHSTGLSEEVAKNIREAGLDVAIF 63
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
P+P+ V E + ++ + + +++GGGS D AKA+ + G D + S
Sbjct: 64 PKAQPDPADTQVHEGVDVFKQENCDALVSIGGGSSHDTAKAIGLVAANGGRINDYQGVNS 123
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKG--DTKYTIVSPELCPDYAILDPR 178
K P+ +AI +T+G GSE ++ +VI K ++ ++ P AI+DP
Sbjct: 124 VEKPVVPV-------VAITTTAGTGSETTSLAVITDSARKVKMPVIDEKITPTVAIVDPE 176
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV-NG-DAK 236
L K P + +G+D L HAIEAYV+K +TP +DA A++A ++I E L +V NG D +
Sbjct: 177 LMVKKPAGLTIATGMDALSHAIEAYVAKGATPVTDAFAIQAMKLINEYLPKAVANGEDIE 236
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG--PHGLVNAIFLPYVMEFNMERARE 294
ARE + YA MAG+AF NG LGLVH++SH+ + HG+ N++ +P+V FN+ E
Sbjct: 237 AREAMAYAQYMAGVAFNNGGLGLVHSISHQVGGVYKLQHGICNSVNMPHVCAFNLIAKTE 296
Query: 295 KYDAMARELG--------LSSADELLEKVKELNEKLGVPK-LSEI-VKEEEFLGKVEDMS 344
++ +A LG ++A+ + ++ N+ G+P +E+ VKEE+ +E ++
Sbjct: 297 RFAHIAELLGENVSGLSTAAAAERAIVALERYNKNFGIPSGYAEMGVKEED----IELLA 352
Query: 345 RKTYEDPLVSFNPVEPSVEDIKNLYLRAL 373
+ +ED NP +V+DI + AL
Sbjct: 353 KNAFEDVCTQSNPRVATVQDIAQIIKNAL 381
>emb|CAA33124.1| (X15025) fucO ORF (AA 1-383) [Escherichia coli]
Length = 383
Score = 175 bits (440), Expect = 6e-43
Identities = 121/386 (31%), Positives = 198/386 (50%), Gaps = 29/386 (7%)
Query: 2 RFFLKTTILMGRGSL----ENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVY 57
R L T GRG++ + VKR ++ LI + +++ + G +++V D ++ AG +
Sbjct: 5 RMILNETAWFGRGAVGALTDEVKR--RGYQKALIVTDKTLVQCGVVAKVTDKMDAAGLAW 62
Query: 58 ETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVA 117
G+ P P++ V+E L + + IA+GGGS D KA+ + + P A
Sbjct: 63 AIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDTCKAIGIISNNP-----EFA 117
Query: 118 IFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAIL 175
+ P K ++AIP+T+G +EV+ VI + K+ V P P A +
Sbjct: 118 DVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRRKFVCVDPHDIPQVAFI 177
Query: 176 DPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDA 235
D + + MP + +G+D L HAIE Y+++ + +DAL +KA II L SV GD
Sbjct: 178 DADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHIKAIEIIAGALRGSVAGDK 237
Query: 236 KAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAR 293
A EE+ +AG+ F N LGLVH M+H + + PHG+ NAI LP+VM +N +
Sbjct: 238 DAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYTTPHGVANAILLPHVMRYNADFTG 297
Query: 294 EKYDAMARELGL--------SSADELLEKVKELNEKLGV-PKLSEI-VKEEEFLGKVEDM 343
EKY +AR +G+ + + +E V LN +G+ P L ++ V++E+ + +
Sbjct: 298 EKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVGIPPHLRDVGVRKED----IPAL 353
Query: 344 SRKTYEDPLVSFNPVEPSVEDIKNLY 369
++ +D NP E ++EDI LY
Sbjct: 354 AQAALDDVCTGGNPREATLEDIVELY 379
>sp|P11549|FUCO_ECOLI LACTALDEHYDE REDUCTASE (PROPANEDIOL OXIDOREDUCTASE)
>gi|65949|pir||RDECLA lactaldehyde reductase (EC
1.1.1.77) - Escherichia coli >gi|146043|gb|AAA23824.1|
(M31059) oxireductase [Escherichia coli]
>gi|146045|gb|AAA23825.1| (M27177) 1,2-propanediol
oxidoreductase [Escherichia coli]
>gi|882694|gb|AAB40449.1| (U29581) 1,2-propanediol
oxidoreductase (lactaldehyde reductase [Escherichia
coli] >gi|1789163|gb|AAC75841.1| (AE000363)
L-1,2-propanediol oxidoreductase [Escherichia coli K12]
Length = 383
Score = 175 bits (438), Expect = 1e-42
Identities = 121/386 (31%), Positives = 198/386 (50%), Gaps = 29/386 (7%)
Query: 2 RFFLKTTILMGRGSL----ENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVY 57
R L T GRG++ + VKR ++ LI + +++ + G +++V D ++ AG +
Sbjct: 5 RMILNETAWFGRGAVGALTDEVKR--RGYQKALIVTDKTLVQCGVVAKVTDKMDAAGLAW 62
Query: 58 ETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVA 117
G+ P P++ V+E L + + IA+GGGS D KA+ + + P A
Sbjct: 63 AIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDTCKAIGIISNNP-----EFA 117
Query: 118 IFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAIL 175
+ P K ++AIP+T+G +EV+ VI + K+ V P P A +
Sbjct: 118 DVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRRKFVCVDPHDIPQVAFI 177
Query: 176 DPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDA 235
D + + MP + +G+D L HAIE Y+++ + +DAL +KA II L SV GD
Sbjct: 178 DADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHIKAIEIIAGALRGSVAGDK 237
Query: 236 KAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAR 293
A EE+ +AG+ F N LGLVH M+H + + PHG+ NAI LP+VM +N +
Sbjct: 238 DAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPHGVANAILLPHVMRYNADFTG 297
Query: 294 EKYDAMARELGL--------SSADELLEKVKELNEKLGV-PKLSEI-VKEEEFLGKVEDM 343
EKY +AR +G+ + + +E V LN +G+ P L ++ V++E+ + +
Sbjct: 298 EKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVGIPPHLRDVGVRKED----IPAL 353
Query: 344 SRKTYEDPLVSFNPVEPSVEDIKNLY 369
++ +D NP E ++EDI LY
Sbjct: 354 AQAALDDVCTGGNPREATLEDIVELY 379
>gi|11497645 alcohol dehydrogenase, iron-containing [Archaeoglobus fulgidus]
>gi|7431294|pir||H69252 alcohol dehydrogenase,
iron-containing homolog - Archaeoglobus fulgidus
>gi|2650627|gb|AAB91203.1| (AE001105) alcohol
dehydrogenase, iron-containing [Archaeoglobus fulgidus]
Length = 416
Score = 174 bits (437), Expect = 1e-42
Identities = 110/323 (34%), Positives = 180/323 (55%), Gaps = 24/323 (7%)
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKK 124
PE + +++ K+E P+ +A+GGGSV+D+AKA + Y+AP D +V
Sbjct: 94 PEVPLSSIKPAAEKMEALQPDLIVAVGGGSVMDMAKASWILYEAPQTDLTNVDAM----- 148
Query: 125 AQPLP-KLKTRLIAIPSTSGAGSEVSAASVIKKGDT----KYTIVSPELCPDYAILDPRL 179
QP+ + K L IP+T+G GSE + +VI K +V PEL PD+A+LDPR
Sbjct: 149 -QPIGLRRKAFLACIPTTAGTGSEATNVAVITDDTVEPHRKEGVVHPELYPDFAVLDPRF 207
Query: 180 PEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVN--GDAKA 237
KMP + +GLD L HA++ Y+ ++S SDALAV+A +++F+ L ++ + +
Sbjct: 208 VLKMPPNLTLGTGLDALSHAVDCYLCRSSNEISDALAVRATKLVFKYLPLALKYPDNPQI 267
Query: 238 REEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREK 295
R + AA +AG AF NG +G HA++H + + HG+ + +PYV++F E +K
Sbjct: 268 RLKTQIAAMIAGSAFSNGGIGTTHAVAHNIGALFEVHHGMACGVTIPYVIKFYAE-VDDK 326
Query: 296 YDAMARELGL--SSADELLEKV----KELNEKLGVPKLSEI--VKEEEFLGKVEDMSRKT 347
Y + A ELG+ S +E L+K+ + K+G P +KE++F K++ ++
Sbjct: 327 YLSFAEELGVRGGSNEETLKKLIDIFMDFYRKIGAPTTMRDLGIKEDDFKEKLDTFAQYV 386
Query: 348 YEDPLVSFNPVEPSVEDIKNLYL 370
+DP F P + +VE+ K L +
Sbjct: 387 VKDPTGPFAPRQLTVEEAKELLM 409
>dbj|BAB04253.1| (AP001508) NAD-dependent methanol dehydrogenase [Bacillus
halodurans]
Length = 382
Score = 173 bits (435), Expect = 2e-42
Identities = 123/362 (33%), Positives = 191/362 (51%), Gaps = 25/362 (6%)
Query: 27 ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPET 86
++VLI S R + G+L +V + +E+ + T +G+ +P VE+ + +
Sbjct: 31 KKVLIVSDRGVANAGWLEKVMELCKESHLPFATFVGVTTDPKDREVEQGCQVFVQHECDA 90
Query: 87 FIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGS 146
I +GGGSV+DVAKA+ + G D I + KK PLP + I I +T+G+GS
Sbjct: 91 IIGVGGGSVLDVAKAIAILATNGGAISDYEGI-DKIKK--PLPPM----IMIMTTAGSGS 143
Query: 147 EVSAASVIKKGDTK--YTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYV 204
EVS S+I + K TIVS L PD AI+DP + +G+DVL HAIEAYV
Sbjct: 144 EVSQFSIIVDSNRKKKMTIVSKTLVPDIAIIDPYTLVTKDSSLTATTGMDVLTHAIEAYV 203
Query: 205 SKASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEVHYAATMAGIAFLNGRLGLVHA 262
S A+TP +D A A + + L SV + +A+E + A+ AG+AF N LG HA
Sbjct: 204 SIAATPLTDVQAKNALILCAQYLRPSVASKTNTEAKEAMAMASLQAGLAFSNAILGAAHA 263
Query: 263 MSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELGL--------SSADELL 312
+SH + PHG +NAI LPYVM+FN +++ +A LG+ + + +
Sbjct: 264 ISHAIGGKFPLPHGEINAILLPYVMDFNFIATPKRFLDIATCLGIDTRGLTEREAGNAAI 323
Query: 313 EKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYLR 371
VKEL+ +G+P + E+ +E + + +++ ED + NP + +VE +K +
Sbjct: 324 HFVKELSFDIGIPTRFREVGITQEMIHHISEVA---LEDACMITNPRDMNVEQVKQVLKN 380
Query: 372 AL 373
L
Sbjct: 381 VL 382
>emb|CAA04467.1| (AJ001008) Alcohol-acetaldehyde dehydrogenase [Lactococcus lactis]
Length = 903
Score = 168 bits (422), Expect = 7e-41
Identities = 120/401 (29%), Positives = 207/401 (50%), Gaps = 61/401 (15%)
Query: 28 RVLIFSSRSMDRLGFLSEVADYVEEAGGVYETII--GLPPEPSVENVEEVLPKVEEFSPE 85
+ I + M + GF+ +V + + ET I + P+P++ + ++++F P+
Sbjct: 503 KAFIVADPGMVKFGFVDKVLEQLAIRPTQVETSIYGSVQPDPTLSEAIAIARQMKQFEPD 562
Query: 86 TFIALGGGSVIDVAKAVKVFY--DAPG-LDF-DSVAIFSRFKK-AQPLPKLKTRLI---- 136
T I LGGGS +D K ++ Y DA G D D ++ F++ AQ ++ R+I
Sbjct: 563 TVICLGGGSALDAGKIGRLIYEYDARGEADLSDDASLKELFQELAQKFVDIRKRIIKFYH 622
Query: 137 -------AIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPEKMPREV 187
AIP+TSG GSEV+ +VI +T KY + +L P AI+DP +P+
Sbjct: 623 PHKAQMVAIPTTSGTGSEVTPFAVITDDETHVKYPLADYQLTPQVAIVDPEFVMTVPKRT 682
Query: 188 ARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGD--------AKARE 239
SG+D + HA+E+YVS S+ ++ ++++A ++IFE L +S + D KARE
Sbjct: 683 VSWSGIDAMSHALESYVSVMSSDYTKPISLQAIKLIFENLTESYHYDPAHPTKEGQKARE 742
Query: 240 EVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG-----PHGLVNAIFLPYVMEFN------ 288
+H AAT+AG+AF N LG+ H+++HK IG PHGL AI +P+V++FN
Sbjct: 743 NMHNAATLAGMAFANAFLGINHSLAHK---IGGEFGLPHGLAIAIAMPHVIKFNAVTGNV 799
Query: 289 ---------MERAREKYDAMARELGLSSADE--------LLEKVKELNEK--LGVPKLSE 329
RA+E Y ++R +G + D+ L+ ++K+L + + +
Sbjct: 800 KRTPYPRYETYRAQEDYAEISRFMGFAGKDDSDEKAVQALVAELKKLTDSIDINITLSGN 859
Query: 330 IVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYL 370
+ + +++ ++ Y+D NP +P +++IK L L
Sbjct: 860 GIDKAHLERELDKLADLVYDDQCTPANPRQPRIDEIKQLLL 900
>emb|CAA04465.1| (AJ001007) Alcohol-acetaldehyde dehydrogenase [Lactococcus lactis]
Length = 903
Score = 166 bits (417), Expect = 3e-40
Identities = 119/398 (29%), Positives = 205/398 (50%), Gaps = 55/398 (13%)
Query: 28 RVLIFSSRSMDRLGFLSEVADYVEEAGGVYETII--GLPPEPSVENVEEVLPKVEEFSPE 85
+ I + M + GF+ +V + + ET I + P+P++ + ++ F P+
Sbjct: 503 KAFIVADPGMVKFGFVDKVLEQLAIRPTQVETSIYGSVQPDPTLSEAIAIARQMNHFEPD 562
Query: 86 TFIALGGGSVIDVAKAVKVFY--DAPG-LDF-DSVAIFSRFKK-AQPLPKLKTRLI---- 136
T I LGGGS +D K ++ Y DA G D D ++ F++ AQ ++ R+I
Sbjct: 563 TVICLGGGSALDAGKIGRLIYEYDARGEADLSDDASLKEIFQELAQKFVDIRKRIIKFYH 622
Query: 137 -------AIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPEKMPREV 187
AIP+TSG GSEV+ +VI +T KY + +L P AI+DP +P+
Sbjct: 623 PHKAQMVAIPTTSGTGSEVTPFAVITDDETHVKYPLADYQLTPQVAIVDPEFVMTVPKRT 682
Query: 188 ARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGD--------AKARE 239
SG+D + HA+E+YVS S+ ++ ++++A ++IFE L +S + D KARE
Sbjct: 683 VSWSGIDAMSHALESYVSVMSSDYTKPISLQAIKLIFENLTESYHYDPAHPTKEGQKARE 742
Query: 240 EVHYAATMAGIAFLNGRLGLVHAMSHKSA--WIGPHGLVNAIFLPYVMEFN--------- 288
+H AAT+AG+AF N LG+ H+++HK A + PHGL AI +P+V++FN
Sbjct: 743 NMHNAATLAGMAFANAFLGINHSLAHKIAGEFGLPHGLAIAIAMPHVIKFNAVTGNVKFT 802
Query: 289 ------MERAREKYDAMARELGLSSADE--------LLEKVKELNEK--LGVPKLSEIVK 332
RA+E Y ++R +G + ++ L+ ++K+L + + + V
Sbjct: 803 PYPRYETYRAQEDYAEISRFMGFAGKEDSDEKAVKALVAELKKLTDSIDINITLSGNGVD 862
Query: 333 EEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYL 370
+ +++ ++ Y+D NP +P +++IK L L
Sbjct: 863 KAHLERELDKLADLVYDDQCTPANPRQPRIDEIKQLLL 900
>sp|P76553|EUTG_ECOLI ETHANOLAMINE UTILIZATION PROTEIN EUTG >gi|1799879|dbj|BAA16331.1|
(D90874) ETHANOLAMINE UTILIZATION PROTEIN EUTG.
[Escherichia coli]
Length = 395
Score = 165 bits (413), Expect = 8e-40
Identities = 117/316 (37%), Positives = 167/316 (52%), Gaps = 19/316 (6%)
Query: 66 EPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKA 125
EP + +V + ++ E + IA GGGSV+D AKAV + P ++A S
Sbjct: 89 EPCITDVCAAVAQLRESGCDGVIAFGGGSVLDAAKAVTLLVTNPD---STLAEMSETSVL 145
Query: 126 QPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPRLPEKM 183
QP + LIAIP+T+G GSE + +VI K + L PD AILD L E +
Sbjct: 146 QP----RLPLIAIPTTAGTGSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGV 201
Query: 184 PREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEV 241
P V +G+D L HAIEAY + +TPF+D+LA+ A +I + L +V D ARE +
Sbjct: 202 PSHVTAMTGIDALTHAIEAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESM 261
Query: 242 HYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAM 299
A+ MAG+AF + LGL HAM+H+ +A PHGL NA+ LP VMEFN RE++ +
Sbjct: 262 LLASCMAGMAFSSAGLGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQI 321
Query: 300 ARELGLSSAD--ELLEKVKELNEKLGVPK-LSEIVKEEEFLGKVEDMSRKTYEDPLVSFN 356
R L +D + + V EL ++G+ K L ++ G ++ ED + N
Sbjct: 322 GRALRTKKSDDRDAINAVSELIAEVGIGKRLGDVGATSAHYGA---WAQAALEDICLRSN 378
Query: 357 PVEPSVEDIKNLYLRA 372
P S+E I LY A
Sbjct: 379 PRTASLEQIVGLYAAA 394
>pir||D65020 ethanolamine utilization protein EutG - Escherichia coli (strain
K-12) >gi|1788795|gb|AAC75506.1| (AE000332) ethanolamine
utilization; homolog of Salmonella enzyme, similar to
iron-containing alcohol dehydrogenase [Escherichia coli
K12]
Length = 404
Score = 165 bits (413), Expect = 8e-40
Identities = 117/316 (37%), Positives = 167/316 (52%), Gaps = 19/316 (6%)
Query: 66 EPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKA 125
EP + +V + ++ E + IA GGGSV+D AKAV + P ++A S
Sbjct: 98 EPCITDVCAAVAQLRESGCDGVIAFGGGSVLDAAKAVTLLVTNPD---STLAEMSETSVL 154
Query: 126 QPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPRLPEKM 183
QP + LIAIP+T+G GSE + +VI K + L PD AILD L E +
Sbjct: 155 QP----RLPLIAIPTTAGTGSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGV 210
Query: 184 PREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEV 241
P V +G+D L HAIEAY + +TPF+D+LA+ A +I + L +V D ARE +
Sbjct: 211 PSHVTAMTGIDALTHAIEAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESM 270
Query: 242 HYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAM 299
A+ MAG+AF + LGL HAM+H+ +A PHGL NA+ LP VMEFN RE++ +
Sbjct: 271 LLASCMAGMAFSSAGLGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQI 330
Query: 300 ARELGLSSAD--ELLEKVKELNEKLGVPK-LSEIVKEEEFLGKVEDMSRKTYEDPLVSFN 356
R L +D + + V EL ++G+ K L ++ G ++ ED + N
Sbjct: 331 GRALRTKKSDDRDAINAVSELIAEVGIGKRLGDVGATSAHYGA---WAQAALEDICLRSN 387
Query: 357 PVEPSVEDIKNLYLRA 372
P S+E I LY A
Sbjct: 388 PRTASLEQIVGLYAAA 403
>sp|P37686|ADH2_ECOLI PROBABLE ALCOHOL DEHYDROGENASE >gi|1073456|pir||S47810 probable
alcohol dehydrogenase (EC 1.1.1.1) - Escherichia coli
>gi|466727|gb|AAB18566.1| (U00039) No definition line
found [Escherichia coli] >gi|1790015|gb|AAC76613.1|
(AE000436) putative oxidoreductase [Escherichia coli
K12]
Length = 382
Score = 164 bits (412), Expect = 1e-39
Identities = 126/385 (32%), Positives = 201/385 (51%), Gaps = 29/385 (7%)
Query: 3 FFLKTTILMGRGSLENVKRIISEG--ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
FF+ + ++G SL + ++++ R LI + + +LG +V +EE
Sbjct: 6 FFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTKLGMAGDVQKALEERNIFSVIY 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G P P+ ENV L ++E + ++ I+LGGGS D AK + + A G D
Sbjct: 66 DGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCAKGIALVA-ANGGDIRDYEGVD 124
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
R K Q LP +IAI +T+G SE++ +I + K IV + P ++ D
Sbjct: 125 RSAKPQ-LP-----MIAINTTAGTASEMTRFCIITDEARHIKMAIVDKHVTPLLSVNDSS 178
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAK 236
L MP+ + +G+D L HAIEAYVS A+TP +DA A+KA +I E L +V +AK
Sbjct: 179 LMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALKAVTMIAENLPLAVEDGSNAK 238
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERARE 294
ARE + YA +AG+AF N LG VHAM+H+ + PHG+ NA+ LP+V FN + A
Sbjct: 239 AREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHGVCNAVLLPHVQVFNSKVAAA 298
Query: 295 KYDAMARELGLS--------SADELLEKVKELNEKLGVPK-LSEI-VKEEEFLGKVEDMS 344
+ A +G++ A+ + ++EL +K+ +P L ++ VKEE+F ++
Sbjct: 299 RLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIPAGLRDLNVKEEDF----AVLA 354
Query: 345 RKTYEDPLVSFNPVEPSVEDIKNLY 369
+D NP++ + E+I +Y
Sbjct: 355 TNALKDACGFTNPIQATHEEIVAIY 379
>dbj|BAA75510.1| (AB023641) alcohol dehydrogenase [Rhodospirillum rubrum]
Length = 383
Score = 164 bits (411), Expect = 1e-39
Identities = 127/390 (32%), Positives = 195/390 (49%), Gaps = 33/390 (8%)
Query: 3 FFLKTTILMGRGSLENVKRIISEG--ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
FF+ + LMG G L I+E +R LI + +++LG +V + +++ G
Sbjct: 6 FFIPSINLMGAGCLATAVNTINERGFKRALIVTDAVLEKLGVAGKVTELLDDHGVESIVF 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGL--DFDSVAI 118
G P P+V NVE L + E + I+LGGGS D AK + + G+ D++ V
Sbjct: 66 GGAQPNPTVSNVEAGLAILREAECDCVISLGGGSPHDCAKGIALCATNGGVIGDYEGVD- 124
Query: 119 FSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGD--TKYTIVSPELCPDYAILD 176
K A P + LIAI +T+G SE++ +I K IV + P ++ D
Sbjct: 125 ----KSAHP----QLPLIAINTTAGTASEMTRFCIITDESRLVKMAIVDRHVTPIMSVND 176
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGD 234
P L P + +G+D L HAIEAYVS A+TP +DA A+KA II L +V D
Sbjct: 177 PELMIGKPAALTAATGMDALTHAIEAYVSIAATPITDACALKAVSIIARTLRRAVADGAD 236
Query: 235 AKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERA 292
KARE + YA +AG+AF N LG VHAM+H+ + PHG+ NA+ LP+V FN A
Sbjct: 237 IKAREAMSYAQFLAGMAFNNASLGFVHAMAHQLGGFYDLPHGVCNAVLLPHVAAFNASVA 296
Query: 293 REKYDAMARELGL--------SSADELLEKVKELNEKLGVPK-LSEI-VKEEEFLGKVED 342
+ +A +G+ A+ + ++ L +G+P L+E+ VK+ G V
Sbjct: 297 GARLKDVAEAMGVDIANLSDDQGAEACIAAIRTLAADIGIPAGLTELGVKD----GDVPT 352
Query: 343 MSRKTYEDPLVSFNPVEPSVEDIKNLYLRA 372
++ +D NP + +I+ ++ A
Sbjct: 353 LAINALKDACGLTNPRPATQAEIEAIFRAA 382
>emb|CAA34911.1| (X17065) alcohol dehydrogenase (AA 1-383) [Zymomonas mobilis]
Length = 383
Score = 162 bits (405), Expect = 7e-39
Identities = 122/387 (31%), Positives = 198/387 (50%), Gaps = 27/387 (6%)
Query: 3 FFLKTTILMGRGSLEN-VKRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F++ MG GSLE +K + G + LI S M++ G + +VAD ++ G
Sbjct: 6 FYIPFVNEMGEGSLEKAIKDLNGSGFKNALIVSDAFMNKSGVVKQVADLLKTQGINSAVY 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ P P+V V E L +++ + + I+LGGGS D AKA+ + G D I
Sbjct: 66 DGVMPNPTVTAVLEGLKILKDNNSDFVISLGGGSPHDCAKAIALVATNGGEVKDYEGIDK 125
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
K A PL + T T+G SE++ +I + K IV + P ++ DP
Sbjct: 126 SKKPALPLMSINT-------TAGTASEMTRFCIITDEVRHVKMAIVDRHVTPMVSVNDPL 178
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DAK 236
L MP+ + +G+D L HA EAY S A+TP +DA A+KAA +I + L+ + + D
Sbjct: 179 LMVGMPKGLTAATGMDALTHAFEAYSSTAATPITDACALKAASMIAKNLKTACDNGKDMP 238
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERARE 294
ARE + YA +AG+AF N LG VHAM+H+ + PHG+ NA+ LP+V+ +N
Sbjct: 239 AREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGYYNLPHGVCNAVLLPHVLAYNASVVAG 298
Query: 295 KYDAMARELGL--------SSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSR 345
+ + +GL A+ ++ V++L +G+P L+E+ ++E + + D +
Sbjct: 299 RLKDVGVAMGLDIANLGDKEGAEATIQAVRDLAASIGIPANLTELGAKKEDVPLLADHAL 358
Query: 346 KTYEDPLVSFNPVEPSVEDIKNLYLRA 372
K D NP + ++++ L+L A
Sbjct: 359 K---DACALTNPRQGDQKEVEELFLSA 382
>sp|P06758|ADH2_ZYMMO ALCOHOL DEHYDROGENASE II (ADH II) >gi|78223|pir||A25978 alcohol
dehydrogenase (EC 1.1.1.1) 2 - Zymomonas mobilis
>gi|155577|gb|AAA27683.1| (M15394) alcohol dehydrogenase
II (EC 1.1.1.1) [Zymomonas mobilis]
Length = 383
Score = 162 bits (405), Expect = 7e-39
Identities = 122/387 (31%), Positives = 198/387 (50%), Gaps = 27/387 (6%)
Query: 3 FFLKTTILMGRGSLEN-VKRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F++ MG GSLE +K + G + LI S M++ G + +VAD ++ G
Sbjct: 6 FYIPFVNEMGEGSLEKAIKDLNGSGFKNALIVSDAFMNKSGVVKQVADLLKAQGINSAVY 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ P P+V V E L +++ + + I+LGGGS D AKA+ + G D I
Sbjct: 66 DGVMPNPTVTAVLEGLKILKDNNSDFVISLGGGSPHDCAKAIALVATNGGEVKDYEGIDK 125
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
K A PL + T T+G SE++ +I + K IV + P ++ DP
Sbjct: 126 SKKPALPLMSINT-------TAGTASEMTRFCIITDEVRHVKMAIVDRHVTPMVSVNDPL 178
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DAK 236
L MP+ + +G+D L HA EAY S A+TP +DA A+KAA +I + L+ + + D
Sbjct: 179 LMVGMPKGLTAATGMDALTHAFEAYSSTAATPITDACALKAASMIAKNLKTACDNGKDMP 238
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERARE 294
ARE + YA +AG+AF N LG VHAM+H+ + PHG+ NA+ LP+V+ +N
Sbjct: 239 AREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGYYNLPHGVCNAVLLPHVLAYNASVVAG 298
Query: 295 KYDAMARELGL--------SSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSR 345
+ + +GL A+ ++ V++L +G+P L+E+ ++E + + D +
Sbjct: 299 RLKDVGVAMGLDIANLGDKEGAEATIQAVRDLAASIGIPANLTELGAKKEDVPLLADHAL 358
Query: 346 KTYEDPLVSFNPVEPSVEDIKNLYLRA 372
K D NP + ++++ L+L A
Sbjct: 359 K---DACALTNPRQGDQKEVEELFLSA 382
>pir||T33729 alcohol dehydrogenase (EC 1.1.1.1) II - Zymomonas mobilis
>gi|3820588|gb|AAC70367.1| (AF086791) alcohol
dehydrogenase II [Zymomonas mobilis]
Length = 383
Score = 161 bits (404), Expect = 1e-38
Identities = 122/387 (31%), Positives = 198/387 (50%), Gaps = 27/387 (6%)
Query: 3 FFLKTTILMGRGSLEN-VKRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F++ MG GSLE +K + G + LI S M++ G + +VAD ++ G
Sbjct: 6 FYIPFVNEMGEGSLEKAIKDLNGSGFKNALIVSDAFMNKSGVVKQVADLLKAQGINSAVY 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ P P+V V E L +++ + + I+LGGGS D AKA+ + G D I
Sbjct: 66 DGVMPNPTVTAVLEGLKILKDNNSDFVISLGGGSPHDCAKAIALVATNGGEVKDYEGIDK 125
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
K A PL + T T+G SE++ +I + K IV + P ++ DP
Sbjct: 126 SKKPALPLMSINT-------TAGTASEMTRFCIITDEVRHVKMAIVDRHVTPMVSVNDPL 178
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DAK 236
L MP+ + +G+D L HA EAY S A+TP +DA A+KAA +I + L+ + + D
Sbjct: 179 LMVGMPKGLTAATGMDALTHAFEAYSSTAATPITDACALKAASMIAKNLKTACDNGKDMP 238
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERARE 294
ARE + YA +AG+AF N LG VHAM+H+ + PHG+ NA+ LP+V+ +N
Sbjct: 239 AREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGYYNLPHGVCNAVLLPHVLAYNASVVAG 298
Query: 295 KYDAMARELGL--------SSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSR 345
+ + +GL A+ ++ V++L +G+P L+E+ ++E + + D +
Sbjct: 299 RLKDVGVAMGLDIANLGDKEGAEATIQAVRDLAAPIGIPANLTELGAKKEDVPLLADHAL 358
Query: 346 KTYEDPLVSFNPVEPSVEDIKNLYLRA 372
K D NP + ++++ L+L A
Sbjct: 359 K---DACALTNPRQGDQKEVEELFLSA 382
>gi|11499601 alcohol dehydrogenase, iron-containing [Archaeoglobus fulgidus]
>gi|7431303|pir||B69502 alcohol dehydrogenase,
iron-containing homolog - Archaeoglobus fulgidus
>gi|2648512|gb|AAB89231.1| (AE000963) alcohol
dehydrogenase, iron-containing [Archaeoglobus fulgidus]
Length = 371
Score = 159 bits (398), Expect = 5e-38
Identities = 125/384 (32%), Positives = 198/384 (51%), Gaps = 41/384 (10%)
Query: 9 ILMGRGSLENVKRIIS--EGERVLIFSSRSMDRLGFLSEVADYVEEAGGV--YETIIGLP 64
++ G G LE ++ + +G +V+ + R++ + VEE YE +
Sbjct: 3 VVFGWGRLERIEEEVERLKGGKVVFIADRAVRKA---------VEELNLSFDYEIFSDVE 53
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKK 124
PEP + V+++ +E+FS +T + +GGGS +DVAK V A G +V + +
Sbjct: 54 PEPEIGIVDDI---IEKFSFDTVVGVGGGSTLDVAKLTAV---AAGEKAKAVELVGK--- 104
Query: 125 AQPLPKLKTRLIAIPSTSGAGSEVSAASVIK--KGDTKYTIVSPELCPDYAILDPRLPEK 182
LP+ K +L+ P+T+G GSEV+ +V K K + KY L D AI+DP+L
Sbjct: 105 --KLPERKCKLLLCPTTAGTGSEVTKLAVFKIPKREVKYVFDDESLYADIAIIDPKLTLS 162
Query: 183 MPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDS-VNGD-AKAREE 240
P V +SG+D HAIEAY S S P SD A KA II + L ++ NG+ +AR +
Sbjct: 163 CPPHVTASSGIDAFCHAIEAYTSLFSNPISDMFAEKAIAIIPKALREAYANGENVRARTD 222
Query: 241 VHYAATMAGIAFLNGRLGLVHAM--SHKSAWIGPHGLVNAIFLPYVMEFNMERAREKYDA 298
+ +A+ +AGIAF + GL HA+ +H PHG I +PYV+++N EK+
Sbjct: 223 MSFASLLAGIAFNSAGTGLGHALGYAHNHIHNSPHGKSVGITMPYVLQYNAIADLEKHAR 282
Query: 299 MARELGLSSADELLEKV-------KELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKTYED 350
+AR LG S L E +L + LG+P KLS++ E+ + K+ D R +
Sbjct: 283 IARALG-ESGSSLRETALRAGIGFAKLLKDLGMPHKLSDVGAGEDDIEKIVD--RIFLSE 339
Query: 351 PLVSFNPVEPSVEDIKNLYLRALH 374
V NP E++ ++ +++H
Sbjct: 340 KHVRRNPRVVRREEMYDVVRKSIH 363
>sp|Q09669|ADHF_SCHPO PUTATIVE IRON ALCOHOL DEHYDROGENASE >gi|1076891|pir||S55484
probable alcohol dehydrogenase (EC 1.1.1.1) - fission
yeast (Schizosaccharomyces pombe)
>gi|854605|emb|CAA89956.1| (Z49811) iron containing
alcohol dehydrogenase [Schizosaccharomyces pombe]
Length = 422
Score = 157 bits (393), Expect = 2e-37
Identities = 114/385 (29%), Positives = 203/385 (52%), Gaps = 25/385 (6%)
Query: 3 FFLKTTILMGRGSL-ENVKRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F++ + L G+G L E K+I G + LI + + ++G +V +EE
Sbjct: 49 FYIPSFNLFGKGCLAEAAKQIKMSGFKNTLIVTDPGIIKVGLYDKVKALLEEQSITVHLY 108
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ P P+V NV + L V++ + ++ +++GGGS D AK + + G D +
Sbjct: 109 DGVQPNPTVGNVNQGLEIVKKENCDSMVSIGGGSAHDCAKGIALLATNGGKIADYEGVDK 168
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPR 178
K PL IAI +T+G SE++ ++I + K I+ P ++ DP
Sbjct: 169 SSKPQLPL-------IAINTTAGTASEMTRFAIITEETRHIKMAIIDKHTMPILSVNDPE 221
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DAK 236
+P + +G+D L HA+EAYVS A+ P +DA AVK ++ + L+ +V+ D +
Sbjct: 222 TMYGLPPSLTAATGMDALTHAVEAYVSTAANPITDACAVKCIELVNKYLKRAVDNGKDEE 281
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHK-SAWIG-PHGLVNAIFLPYVMEFNME--RA 292
AR+ + YA + G+AF N LG VHAM+H+ + G PHG+ NA+ L +V +FN RA
Sbjct: 282 ARDNMAYAEFLGGMAFNNASLGYVHAMAHQLGGFYGIPHGVCNAVLLAHVQKFNSRDPRA 341
Query: 293 REKYDAMARELGLS--SADELLEKVKELNEKLGV-PKLSEI-VKEEEFLGKVEDMSRKTY 348
+ +A LG +A+ L+++ +L ++ + P L ++ VKE++F + +
Sbjct: 342 NARLGDIAFHLGCEEHTAEAALDRISQLVLEVKIRPHLVDLGVKEKDF----DVLVDHAM 397
Query: 349 EDPLVSFNPVEPSVEDIKNLYLRAL 373
+D + NP++P+ +++K ++ A+
Sbjct: 398 KDACGATNPIQPTHDEVKAIFKSAM 422
>sp|P33744|ADHE_CLOAB ALDEHYDE-ALCOHOL DEHYDROGENASE [INCLUDES: ALCOHOL DEHYDROGENASE
(ADH); ACETALDEHYDE DEHYDROGENASE [ACETYLATING] (ACDH)]
Length = 872
Score = 156 bits (390), Expect = 4e-37
Identities = 112/378 (29%), Positives = 192/378 (50%), Gaps = 34/378 (8%)
Query: 27 ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPET 86
+R I + L ++ + +E ++ + E ++ +++ ++ F P+T
Sbjct: 478 KRAFIVTDSDPYNLNYVDSIIKILEHLDIDFKVFNKVGREADLKTIKKATEEMSSFMPDT 537
Query: 87 FIALGGGSVIDVAKAVKVFYDAPGLDFDSVAI--FSRFKKAQPLPKL--KTRLIAIPSTS 142
IALGG + AK + V Y+ P + F+ +AI K+ PKL K L+AI +++
Sbjct: 538 IIALGGTPEMSSAKLMWVLYEHPEVKFEDLAIKFMDIRKRIYTFPKLGKKAMLVAITTSA 597
Query: 143 GAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAI 200
G+GSEV+ +++ +T KY + E+ P+ AI+D L KMP+ + SG+D LV++I
Sbjct: 598 GSGSEVTPFALVTDNNTGNKYMLADYEMTPNMAIVDAELMMKMPKGLTAYSGIDALVNSI 657
Query: 201 EAYVSKASTPFSDALAVKAARIIFERLEDSV-NG--DAKAREEVHYAATMAGIAFLNGRL 257
EAY S ++ +++ LA++A R+IF+ L ++ NG + KARE++ +A+TMAG+A N L
Sbjct: 658 EAYTSVYASEYTNGLALEAIRLIFKYLPEAYKNGRTNEKAREKMAHASTMAGMASANAFL 717
Query: 258 GLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFN--------------------MERAR-E 294
GL H+M+ K S P G+ NA+ + V++FN AR
Sbjct: 718 GLCHSMAIKLSSEHNIPSGIANALLIEEVIKFNAVDNPVKQAPCPQYKYPNTIFRYARIA 777
Query: 295 KYDAMARELGLSSADELLEKVKELNEKLGVPKLSEI--VKEEEFLGKVEDMSRKTYEDPL 352
Y + D L+ K+ EL + L +P + V EE F ++ +S +D
Sbjct: 778 DYIKLGGNTDEEKVDLLINKIHELKKALNIPTSIKDAGVLEENFYSSLDRISELALDDQC 837
Query: 353 VSFNPVEPSVEDIKNLYL 370
NP P +IK +Y+
Sbjct: 838 TGANPRFPLTSEIKEMYI 855
>pir||A49346 aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) / alcohol dehydrogenase
(EC 1.1.1.1) E - Clostridium acetobutylicum
>gi|298083|emb|CAA51344.1| (X72831) alcohol
dehydrogenase E [Clostridium acetobutylicum]
>gi|3790107|gb|AAD04638.1| (L14817) aldehyde-alcohol
dehydrogenase [Clostridium acetobutylicum]
Length = 862
Score = 156 bits (390), Expect = 4e-37
Identities = 112/378 (29%), Positives = 192/378 (50%), Gaps = 34/378 (8%)
Query: 27 ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPET 86
+R I + L ++ + +E ++ + E ++ +++ ++ F P+T
Sbjct: 478 KRAFIVTDSDPYNLNYVDSIIKILEHLDIDFKVFNKVGREADLKTIKKATEEMSSFMPDT 537
Query: 87 FIALGGGSVIDVAKAVKVFYDAPGLDFDSVAI--FSRFKKAQPLPKL--KTRLIAIPSTS 142
IALGG + AK + V Y+ P + F+ +AI K+ PKL K L+AI +++
Sbjct: 538 IIALGGTPEMSSAKLMWVLYEHPEVKFEDLAIKFMDIRKRIYTFPKLGKKAMLVAITTSA 597
Query: 143 GAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAI 200
G+GSEV+ +++ +T KY + E+ P+ AI+D L KMP+ + SG+D LV++I
Sbjct: 598 GSGSEVTPFALVTDNNTGNKYMLADYEMTPNMAIVDAELMMKMPKGLTAYSGIDALVNSI 657
Query: 201 EAYVSKASTPFSDALAVKAARIIFERLEDSV-NG--DAKAREEVHYAATMAGIAFLNGRL 257
EAY S ++ +++ LA++A R+IF+ L ++ NG + KARE++ +A+TMAG+A N L
Sbjct: 658 EAYTSVYASEYTNGLALEAIRLIFKYLPEAYKNGRTNEKAREKMAHASTMAGMASANAFL 717
Query: 258 GLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFN--------------------MERAR-E 294
GL H+M+ K S P G+ NA+ + V++FN AR
Sbjct: 718 GLCHSMAIKLSSEHNIPSGIANALLIEEVIKFNAVDNPVKQAPCPQYKYPNTIFRYARIA 777
Query: 295 KYDAMARELGLSSADELLEKVKELNEKLGVPKLSEI--VKEEEFLGKVEDMSRKTYEDPL 352
Y + D L+ K+ EL + L +P + V EE F ++ +S +D
Sbjct: 778 DYIKLGGNTDEEKVDLLINKIHELKKALNIPTSIKDAGVLEENFYSSLDRISELALDDQC 837
Query: 353 VSFNPVEPSVEDIKNLYL 370
NP P +IK +Y+
Sbjct: 838 TGANPRFPLTSEIKEMYI 855
>emb|CAA29410.1| (X05992) ADH4 (AA 1-382) [Saccharomyces cerevisiae]
Length = 382
Score = 153 bits (384), Expect = 2e-36
Identities = 117/384 (30%), Positives = 188/384 (48%), Gaps = 25/384 (6%)
Query: 3 FFLKTTILMGRGSLENVKRIISEGE--RVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F++ G G+LE I + + LI + + +G V +EE G
Sbjct: 7 FYIPPISFFGEGALEETADYIKNKDYKKALIVTDPGIAAIGLSGRVQKMLEERGLNVAIY 66
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
P P++ NV L ++E + E +++GGGS D AKA+ + G D +
Sbjct: 67 DKTQPNPNIANVTAGLKVLKEENSEIVVSIGGGSAHDNAKAIALLATNGGEIGDYEGVNQ 126
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKY--TIVSPELCPDYAILDPR 178
K A PL AI +T+G SE++ ++I + K I+ + P A+ DP
Sbjct: 127 SKKAALPL-------FAINTTAGTASEMTRFTIISNEEKKIKMAIIDNNVTPAVAVNDPS 179
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DAK 236
+P + +GLD L H IEAYVS AS P +DA A+K +I E L + D K
Sbjct: 180 TMFGLPPALTAATGLDALTHCIEAYVSTASNPITDACALKGIDLINESLVAAYKDGKDKK 239
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNME--RA 292
AR ++ YA +AG+AF N LG VHA++H+ + PHG+ NA+ LP+V E NM+ +A
Sbjct: 240 ARTDMCYAEYLAGMAFNNASLGYVHALAHQLGGFYHLPHGVCNAVLLPHVQEANMQCPKA 299
Query: 293 REKYDAMARELGLSSAD--ELLEKVKELNEKLGVPK-LSEI-VKEEEFLGKVEDMSRKTY 348
+++ +A G S D E ++ + LN + +P+ L ++ VK E+F + ++
Sbjct: 300 KKRLGEIALHCGASQEDPEETIKALHVLNRTMNIPRNLKDLGVKTEDF----DILAEHAM 355
Query: 349 EDPLVSFNPVEPSVEDIKNLYLRA 372
D NPV+ + E + + +A
Sbjct: 356 HDACHLTNPVQFTKEQVVAIIKKA 379
>gi|6321181 alcohol dehydrogenase isoenzyme IV; Adh4p [Saccharomyces
cerevisiae] >gi|1703177|sp|P10127|ADH4_YEAST ALCOHOL
DEHYDROGENASE IV >gi|2144321|pir||DEBY4 alcohol
dehydrogenase (EC 1.1.1.1) 4 - yeast (Saccharomyces
cerevisiae) >gi|1150583|emb|CAA64131.1| (X94357) ORF
NRC465; EMBL:SCADH4;X05992; PIR:DEBY4;S07614
[Saccharomyces cerevisiae] >gi|1322937|emb|CAA96976.1|
(Z72778) ORF YGL256w [Saccharomyces cerevisiae]
Length = 465
Score = 153 bits (384), Expect = 2e-36
Identities = 118/384 (30%), Positives = 187/384 (47%), Gaps = 25/384 (6%)
Query: 3 FFLKTTILMGRGSLENVKRIISEGE--RVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F++ G G+LE I + + LI + + +G V +EE
Sbjct: 90 FYIPPISFFGEGALEETADYIKNKDYKKALIVTDPGIAAIGLSGRVQKMLEERDLNVAIY 149
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
P P++ NV L ++E + E +++GGGS D AKA+ + G D +
Sbjct: 150 DKTQPNPNIANVTAGLKVLKEQNSEIVVSIGGGSAHDNAKAIALLATNGGEIGDYEGVNQ 209
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKY--TIVSPELCPDYAILDPR 178
K A PL AI +T+G SE++ ++I + K I+ + P A+ DP
Sbjct: 210 SKKAALPL-------FAINTTAGTASEMTRFTIISNEEKKIKMAIIDNNVTPAVAVNDPS 262
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DAK 236
+P + +GLD L H IEAYVS AS P +DA A+K +I E L + D K
Sbjct: 263 TMFGLPPALTAATGLDALTHCIEAYVSTASNPITDACALKGIDLINESLVAAYKDGKDKK 322
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNME--RA 292
AR ++ YA +AG+AF N LG VHA++H+ + PHG+ NA+ LP+V E NM+ +A
Sbjct: 323 ARTDMCYAEYLAGMAFNNASLGYVHALAHQLGGFYHLPHGVCNAVLLPHVQEANMQCPKA 382
Query: 293 REKYDAMARELGLSSAD--ELLEKVKELNEKLGVPK-LSEI-VKEEEFLGKVEDMSRKTY 348
+++ +A G S D E ++ + LN + +P+ L E+ VK E+F E ++
Sbjct: 383 KKRLGEIALHFGASQEDPEETIKALHVLNRTMNIPRNLKELGVKTEDF----EILAEHAM 438
Query: 349 EDPLVSFNPVEPSVEDIKNLYLRA 372
D NPV+ + E + + +A
Sbjct: 439 HDACHLTNPVQFTKEQVVAIIKKA 462
>gb|AAC45651.1| (AF006034) 1,3-propanediol dehydrogenase [Clostridium pasteurianum]
Length = 385
Score = 153 bits (384), Expect = 2e-36
Identities = 116/389 (29%), Positives = 199/389 (50%), Gaps = 29/389 (7%)
Query: 3 FFLKTTILMGRGSLENV--KRIISEGERVLIFSSRSMDRL--GFLSEVADYVEEAGGVYE 58
F MG G+++ V + I G++ LI + + + + G +++ Y++EAG
Sbjct: 6 FLAPNVNFMGAGAIKLVGERCKILGGKKALIVTDKFLRNMEDGAVAQTVKYIKEAGIDVA 65
Query: 59 TIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAI 118
+ P P NV + L + + + + +GGGS D K + + G +D I
Sbjct: 66 FYDDVEPNPKDTNVRDGLKVYRKENCDLIVTVGGGSSHDCGKGIGIAATHEGDLYDYAGI 125
Query: 119 FSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTK--YTIVSPELCPDYAILD 176
+ PLP + +A+ +T+G GSEV+ VI TK + IVS P +I D
Sbjct: 126 ETL---TNPLPPI----VAVNTTAGTGSEVTRHCVITNTKTKIKFVIVSWRNLPLVSIND 178
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGD 234
P L K P + +G+D L HAIE+YVSK + P +DALA++A ++I L +V +
Sbjct: 179 PILMIKKPAGLTAATGMDALTHAIESYVSKDANPVTDALAIQAIKLIANNLRQAVALGEN 238
Query: 235 AKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERA 292
+ARE + YA+ +AG+AF N LG VHAM+H+ + HG+ NA+ LP+V +N+
Sbjct: 239 LEARENMAYASLLAGMAFNNANLGYVHAMAHQLGGLYDMAHGVANAMLLPHVERYNLISN 298
Query: 293 REKYDAMARELG--------LSSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDM 343
+K+ +A +G + +A++ ++ + L++ +G+P L E+ E G E M
Sbjct: 299 PKKFADIAEFMGENIEGLSVMEAAEKAIDAMFRLSKDVGIPASLKEMGVNE---GDFEYM 355
Query: 344 SRKTYEDPLVSFNPVEPSVEDIKNLYLRA 372
++ +D NP + + +DI ++ A
Sbjct: 356 AKMALKDGNAFSNPRKGNEKDIVKIFREA 384
>gb|AAD43989.1|U59485_16 (U59485) AttL [Agrobacterium tumefaciens]
Length = 386
Score = 153 bits (382), Expect = 4e-36
Identities = 117/365 (32%), Positives = 187/365 (51%), Gaps = 29/365 (7%)
Query: 25 EGERVLIFSSRSMDRLGFLSEVADYVEEAG---GVYETIIGLPPEPSVENVEEVLPKVEE 81
+ + L+ + + + G + +AD + +G +++ ++ PPE V + E +
Sbjct: 33 QARKALLITDAGLIKAGLIKPIADALAASGFNVTIFDKVVADPPEHIVADCVE---SARQ 89
Query: 82 FSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPST 141
+ I LGGGS +D+AK V V + D I + K LP L+ +P+T
Sbjct: 90 IGVDIVIGLGGGSSLDIAKLVAVLLVSDQTLADMYGIGN--VKGSRLP-----LVLVPTT 142
Query: 142 SGAGSEVSAASVIKKGDT-KYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAI 200
+G GSEV+ S+I G+T K +VSP+L D+ +LD L +P+ +G+D +VHAI
Sbjct: 143 AGTGSEVTNISIITTGETTKMGVVSPQLYADFVLLDAELTVGLPQVHTAATGIDAMVHAI 202
Query: 201 EAYVSK-ASTPFSDALAVKAARIIFERLEDSV-NG-DAKAREEVHYAATMAGIAFLNGRL 257
EAY SK P SDALA +A R++ L + NG D KARE + AT+AG AF N +
Sbjct: 203 EAYTSKHKKNPLSDALAREALRLLGANLIAACRNGADRKAREGMLLGATLAGQAFANSPV 262
Query: 258 GLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELGL-------SSA 308
VHA+++ + PHGL NA+ L V+ FN + A Y +A LG+ + +
Sbjct: 263 AAVHALAYPLGGHYHVPHGLSNALMLGPVLRFNAKAAASLYAELADVLGVPGEGDAATRS 322
Query: 309 DELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKN 367
D ++ ++ L ++ G P +L ++ + L + + K + L+ NPVE ED
Sbjct: 323 DAFVQHMETLMDESGAPRRLRDVGVTDNTLAMLASDAMK--QSRLLVNNPVEVREEDALA 380
Query: 368 LYLRA 372
LY A
Sbjct: 381 LYREA 385
>pir||A82347 iron-containing alcohol dehydrogenase family protein RfbM VC0250
[imported] - Vibrio cholerae (group O1 strain N16961)
>gi|9654659|gb|AAF93426.1| (AE004113) iron-containing
alcohol dehydrogenase family protein RfbM [Vibrio
cholerae]
Length = 374
Score = 150 bits (376), Expect = 2e-35
Identities = 104/315 (33%), Positives = 165/315 (52%), Gaps = 18/315 (5%)
Query: 66 EPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKA 125
EPSVE + V+ ++E+F P+ +ALGGGS+ID AK +FY+ P L +S ++ F A
Sbjct: 71 EPSVEELSGVIGELEDFKPDYILALGGGSIIDGAKLAWLFYECPTL--ESELLYRPF--A 126
Query: 126 QPLPKLKTRLIAIPSTSGAGSEVSAASVIKKG--DTKYTIVSPELCPDYAILDPRLPEKM 183
P + + + AIP+T GAGSEVS+A+V+ ++K +V+ + PD ILDP L ++
Sbjct: 127 LPSLRGRAKFAAIPTTVGAGSEVSSAAVMLDSVTNSKKAVVTHDFLPDLVILDPDLVSEV 186
Query: 184 PREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLE--DSVNGDAKAREEV 241
P +V + + D L HAIE YVS P + A +A II+ + N ++ E+
Sbjct: 187 PPKVLKTTVADALSHAIEGYVSLIDNPLMNTFAEQAVSIIYRYRHQFSNDNWSSEMLSEL 246
Query: 242 HYAATMAGIAFLNGRLGLVHAMSHKSAWIG-PHGLVNAIFLPYVMEFNMER--AREKYDA 298
AA AG + +GL HA++H+ HGL N + +P V+ FN A KY+
Sbjct: 247 QQAAMFAGWVQNHCIVGLSHAIAHQLGTFNIGHGLANGLLMPAVINFNYREHTAATKYEQ 306
Query: 299 MARELGLSSADELLEKVKELNEKLGVPKLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPV 358
+ + GL + + LLE + L +G K E++ + ++ DP NPV
Sbjct: 307 LIYKSGLPNKEALLELFEVL---VGEQKTDMHFSEQQ----LTTIAENALLDPAAKSNPV 359
Query: 359 EPSVEDIKNLYLRAL 373
+ D+K + + L
Sbjct: 360 RFTENDVKEIVKQCL 374
>sp|Q59477|DHAT_KLEPN 1,3-PROPANEDIOL DEHYDROGENASE (3-HYDROXYPROPIONALDEHYDE REDUCTASE)
(1,3-PROPANEDIOL OXIDOREDUCTASE)
>gi|940440|gb|AAA74260.1| (U30903) 1,3-propanediol
oxidoreductase [Klebsiella pneumoniae]
Length = 387
Score = 150 bits (374), Expect = 3e-35
Identities = 107/365 (29%), Positives = 185/365 (50%), Gaps = 29/365 (7%)
Query: 26 GERVLIFSSRSMDRL--GFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFS 83
G++ L+ + + + + G + + Y+ EAG G+ P P NV + L
Sbjct: 33 GKKALLVTDKGLRAIKDGAVDKTLHYLREAGIEVAIFDGVEPNPKDTNVRDGLAVFRREQ 92
Query: 84 PETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSG 143
+ + +GGGS D K + + G + I + PLP + +A+ +T+G
Sbjct: 93 CDIIVTVGGGSPHDCGKGIGIAATHEGDLYQYAGIETL---TNPLPPI----VAVNTTAG 145
Query: 144 AGSEVSAASVIKKGDTK--YTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIE 201
SEV+ V+ +TK + IVS P +I DP L P + +G+D L HA+E
Sbjct: 146 TASEVTRHCVLTNTETKVKFVIVSWRNLPSVSINDPLLMIGKPAALTAATGMDALTHAVE 205
Query: 202 AYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEVHYAATMAGIAFLNGRLGL 259
AY+SK + P +DA A++A R+I L +V + +ARE + YA+ +AG+AF N LG
Sbjct: 206 AYISKDANPVTDAAAMQAIRLIARNLRQAVALGSNLQARENMAYASLLAGMAFNNANLGY 265
Query: 260 VHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELG--------LSSAD 309
VHAM+H+ + PHG+ NA+ LP+V +N+ EK+ +A +G L +A+
Sbjct: 266 VHAMAHQLGGLYDMPHGVANAVLLPHVARYNLIANPEKFADIAELMGENITGLSTLDAAE 325
Query: 310 ELLEKVKELNEKLGVPK-LSEI-VKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKN 367
+ + + L+ +G+P+ L ++ VKE +F M+ +D NP + + ++I
Sbjct: 326 KAIAAITRLSMDIGIPQHLRDLGVKEADF----PYMAEMALKDGNAFSNPRKGNEQEIAA 381
Query: 368 LYLRA 372
++ +A
Sbjct: 382 IFRQA 386
>pir||A72317 alcohol dehydrogenase, iron-containing - Thermotoga maritima
(strain MSB8) >gi|4981457|gb|AAD36001.1|AE001756_4
(AE001756) alcohol dehydrogenase, iron-containing
[Thermotoga maritima]
Length = 359
Score = 149 bits (372), Expect = 5e-35
Identities = 123/384 (32%), Positives = 188/384 (48%), Gaps = 43/384 (11%)
Query: 3 FFLKTTILMGRGSLENVKRIISE-GERVLIFSSRSMDRL-GFLSEVADYVEEAGGVYETI 60
F++ T + G LE II G+R L+ + +S + G L ++ ++E YE
Sbjct: 4 FYMPTDVFFGEKILEKRGNIIDLLGKRALVVTGKSSSKKNGSLDDLKKLLDETEISYEIF 63
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
+ PS +NV + + + S + + LGGGS +D AKAV V L + ++
Sbjct: 64 DEVEENPSFDNVMKAVERYRNDSFDFVVGLGGGSPMDFAKAVAVLLKEKDLSVED--LYD 121
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI------KKGDTKYTIVSPELCPDYAI 174
R K LP ++ IP+T+G GSEV+ S++ K+G T + P YA
Sbjct: 122 REKVKHWLP-----VVEIPTTAGTGSEVTPYSILTDPEGNKRGCTL-------MFPVYAF 169
Query: 175 LDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGD 234
LDPR M E+ ++G+D L HA+E Y+S+ STP SDALA++A +II L ++ G+
Sbjct: 170 LDPRYTYSMSDELTLSTGVDALSHAVEGYLSRKSTPPSDALAIEAMKIIHRNLPKAIEGN 229
Query: 235 AKAREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG--PHGLVNAIFLPYVMEFNMERA 292
+AR+++ A+ +AG+ L HA+ + HG + LP+VME E
Sbjct: 230 REARKKMFVASCLAGMVIAQTGTTLAHALGYPLTTEKGIKHGKATGMVLPFVMEVMKEEI 289
Query: 293 REKYDAMARELGLSSADELLEKVKELN--EKLGVPKLSEIVKEEEFLGKVEDMSR-KTYE 349
EK D + G S LL+ +KEL EK+ V EE VE SR K +
Sbjct: 290 PEKVDTVNHIFGGS----LLKFLKELGLYEKVA-------VSSEELEKWVEKGSRAKHLK 338
Query: 350 DPLVSFNPVEPSVEDIKNLYLRAL 373
+ +F P E I+N+Y AL
Sbjct: 339 NTPGTFTP-----EKIRNIYREAL 357
>sp|P45513|DHAT_CITFR 1,3-PROPANEDIOL DEHYDROGENASE (3-HYDROXYPROPIONALDEHYDE REDUCTASE)
(1,3-PROPANEDIOL OXIDOREDUCTASE) >gi|1360956|pir||A56275
1,3-propanediol dehydrogenase (EC 1.1.1.202) -
Citrobacter freundii >gi|493086|gb|AAB48848.1| (U09771)
1,3-propanediol dehydrogenase [Citrobacter freundii]
Length = 387
Score = 148 bits (369), Expect = 1e-34
Identities = 108/365 (29%), Positives = 184/365 (49%), Gaps = 29/365 (7%)
Query: 26 GERVLIFSSRSMDRL--GFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFS 83
G++ L+ + + + + G + + ++ EAG G+ P P NV + L +
Sbjct: 33 GKKALLVTDKGLRAIKDGAVDKTLTHLREAGIDVVVFDGVEPNPKDTNVRDGLEVFRKEH 92
Query: 84 PETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSG 143
+ + +GGGS D K + + G D S A PLP + +A+ +T+G
Sbjct: 93 CDIIVTVGGGSPHDCGKGIGIAATHEG-DLYSYAGIETL--TNPLPPI----VAVNTTAG 145
Query: 144 AGSEVSAASVIKKGDTK--YTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIE 201
SEV+ V+ TK + IVS P +I DP L P + +G+D L HA+E
Sbjct: 146 TASEVTRHCVLTNTKTKVKFVIVSWRNLPSVSINDPLLMLGKPAPLTAATGMDALTHAVE 205
Query: 202 AYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEVHYAATMAGIAFLNGRLGL 259
AY+SK + P +DA A++A R+I L +V + KARE + YA+ +AG+AF N LG
Sbjct: 206 AYISKDANPVTDAAAIQAIRLIARNLRQAVALGSNLKARENMAYASLLAGMAFNNANLGY 265
Query: 260 VHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELG--------LSSAD 309
VHAM+H+ + PHG+ NA+ LP+V +N+ EK+ +A +G + +A+
Sbjct: 266 VHAMAHQLGGLYDMPHGVANAVLLPHVARYNLIANPEKFADIAEFMGENTDGLSTMDAAE 325
Query: 310 ELLEKVKELNEKLGVPK-LSEI-VKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKN 367
+ + L+ +G+P+ L ++ VKE +F M+ +D NP + + ++I
Sbjct: 326 LAIHAIARLSADIGIPQHLRDLGVKEADF----PYMAEMALKDGNAFSNPRKGNEKEIAE 381
Query: 368 LYLRA 372
++ +A
Sbjct: 382 IFRQA 386
>pir||H82236 iron-containing alcohol dehydrogenase VC1147 [imported] - Vibrio
cholerae (group O1 strain N16961)
>gi|9655620|gb|AAF94306.1| (AE004194) iron-containing
alcohol dehydrogenase [Vibrio cholerae]
Length = 422
Score = 147 bits (367), Expect = 2e-34
Identities = 111/349 (31%), Positives = 179/349 (50%), Gaps = 32/349 (9%)
Query: 2 RFFLKTTILMGRGSLENVKRIISE-GERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
+F T I+ G GSLE+ IIS+ G VL+ S ++ +R L+ Y+++ Y+ +
Sbjct: 10 QFMTATRIIFGEGSLESSLSIISQYGYSVLLVSGKNAERYAPLTA---YLQQQNMRYQHV 66
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
+ + EP++ VEE F P+ IA+GGGSV+D+ KAV G +D V +
Sbjct: 67 V-VSGEPNITMVEEAAMLGRRFKPDMVIAIGGGSVLDMGKAVAAIIPNQGNVYDYVEVVG 125
Query: 121 RF--KKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGD--TKYTIVSPELCPDYAILD 176
R KA+PLP IAIP+T+ +GSEV+ +V+K K ++ SPE+ D AI+D
Sbjct: 126 RSVPLKAKPLP-----FIAIPTTASSGSEVTRNAVLKSAQDRVKISLRSPEMLADVAIID 180
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFE-RLEDSVNGDA 235
P L + +D H +EAYV P +D + + R + + + D
Sbjct: 181 PTLTYGTDPVTSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRRLSRAMIAGCLRDDK 240
Query: 236 KAREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG----PHGLVNAIFLPYVMEFNMER 291
KAR ++ +AA + G+A N +LG H ++ SA G PH ++ A P VM N++
Sbjct: 241 KARSDLSFAAMLGGMATTNAKLGAAHGLA--SALGGKLDAPHSVITARLAPIVMRENIDV 298
Query: 292 ARE--------KYDAMAREL-GLSSA--DELLEKVKELNEKLGVPKLSE 329
A+E +Y ++R L G +A ++ + V + EKL +P L++
Sbjct: 299 AKEAGRSDILQRYKIVSRILTGRKNAGVEDGILWVNMMLEKLSIPNLTD 347
>gb|AAC98741.1| (AF052750) alcohol dehydrogenase [Pseudomonas putida]
Length = 382
Score = 146 bits (366), Expect = 3e-34
Identities = 106/323 (32%), Positives = 167/323 (50%), Gaps = 24/323 (7%)
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKK 124
PEP + VE+ E + I LGGGS ID+AK V F G D + +K
Sbjct: 68 PEPEISLVEDCTRAFREGGHDGLIGLGGGSAIDIAKGVAAFAGHDGALADLFGVDQVSRK 127
Query: 125 AQPLPKLKTRLIAIPSTSGAGSEVSA--ASVIKKGDTKYTIVSPELCPDYAILDPRLPEK 182
PL IAIP+T+G GSEV + +K ++ +VS L PD A++ P L
Sbjct: 128 GPPL-------IAIPTTAGTGSEVLTWRSFPTRKRSSRKALVSDYLLPDVALVSPGLDAH 180
Query: 183 MPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DAKAREE 240
+P V+R SG+D LVHAIEAY+S +P +DA+A+ A ++I L + D ARE+
Sbjct: 181 LPTNVSRASGVDALVHAIEAYLSINRSPITDAIALGAIKLIARALPKAYANPDDLAARED 240
Query: 241 VHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDA 298
+ A+ M G+AF N +G VHA+++ + HG+ NA+ LP+VM +N E+
Sbjct: 241 MATASLMGGMAFGNAGVGAVHALAYPLGGRFNIAHGVSNALLLPHVMRWNKLACTERLRD 300
Query: 299 MARELGLS--------SADELLEKVKELNEKLGVPK-LSEIVKEEEFLGKVEDMSRKTYE 349
+A+ +G++ +AD ++ + EL + +P L ++ L + + + K
Sbjct: 301 VAQAMGVNIAGLSDSQAADASVDAMAELCAAVDIPTGLRAFNVPQDALAPMAEEASKI-- 358
Query: 350 DPLVSFNPVEPSVEDIKNLYLRA 372
D L+ NP + + DI+ +Y A
Sbjct: 359 DRLMRNNPRKFTAGDIEAIYRAA 381
>pir||G83397 probable iron-containing alcohol dehydrogenase PA1991 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9947992|gb|AAG05379.1|AE004625_9 (AE004625) probable
iron-containing alcohol dehydrogenase [Pseudomonas
aeruginosa]
Length = 387
Score = 145 bits (363), Expect = 6e-34
Identities = 114/392 (29%), Positives = 188/392 (47%), Gaps = 31/392 (7%)
Query: 1 MRFFLKTTILMGRGSLENVKRIISE--GERVLIFSSRSMDRLGFLSEVADYVEEAGGVYE 58
+R F+ I+ G G +V + +VL+ S + G+++++ +E G Y
Sbjct: 8 LRKFVSPEIIFGAGCRHSVANYVKTFGARKVLLVSDPGVLAAGWVADIQASLEGQGIDYC 67
Query: 59 TIIGLPPEPSVENVEEVLPKVEEFSPE---TFIALGGGSVIDVAKAVKVF--YDAPGLDF 113
+ P P V+EV+ E + E +A+GGGS +D AK + + + L+F
Sbjct: 68 LYTDVSPNP---RVDEVMLGAEFYRSEGCNVIVAVGGGSPMDCAKGIGIVAAHGRSILEF 124
Query: 114 DSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPD 171
+ V R + P LI IP+T+G ++VS +I D K+++VS + PD
Sbjct: 125 EGV---DRLRVPSP------PLILIPTTAGTSADVSQFVIISNQDERMKFSVVSKAVVPD 175
Query: 172 YAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV 231
+++DP M ++ +G+D LVHAIEA+VS P +D A++A R+I L +
Sbjct: 176 VSLIDPETTLTMDPFLSACTGIDALVHAIEAFVSTGHGPLTDPHALEAMRLIDGNLVQMI 235
Query: 232 --NGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK-SAWIG-PHGLVNAIFLPYVMEF 287
GD RE++ + AG+AF N LG VHAMSH ++ PHGL NA + +V+ F
Sbjct: 236 ANPGDIALREKIMLGSMQAGLAFSNAILGAVHAMSHSLGGYLDLPHGLCNAALVEHVVAF 295
Query: 288 NMERAREKYDAMARELGLSSADELLEKVKE------LNEKLGVPKLSEIVKEEEFLGKVE 341
N A E++ +A G+ +V++ + K V + +
Sbjct: 296 NYSAAPERFKVIAETFGIDCRGLTQGQVRQRLVDHLIAFKHAVGFHESLALHGVGSSDIP 355
Query: 342 DMSRKTYEDPLVSFNPVEPSVEDIKNLYLRAL 373
+SR +DP + NP E S D++ +Y AL
Sbjct: 356 FLSRHAMQDPCILTNPRESSQRDVEVVYGEAL 387
>pir||F82998 probable iron-containing alcohol dehydrogenase PA5186 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9951490|gb|AAG08571.1|AE004931_8 (AE004931) probable
iron-containing alcohol dehydrogenase [Pseudomonas
aeruginosa]
Length = 387
Score = 145 bits (362), Expect = 8e-34
Identities = 124/391 (31%), Positives = 188/391 (47%), Gaps = 31/391 (7%)
Query: 3 FFLKTTILMGRGSLENVKRIISE--GERVLIFSSRSMDRLGFLSEVADYVEEAG---GVY 57
F IL GS + + E RVLI + + RLG L + G VY
Sbjct: 6 FATTAQILCEAGSAIRLAELCRERRARRVLIVTDPGITRLGLLDAILPGFPRLGLAVEVY 65
Query: 58 ETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVA 117
+ ++ PPE VE L + + E + GGGS +DVAK V + P
Sbjct: 66 DQVLADPPESVVE---AALGRARGMAAELVVGFGGGSSMDVAKLVALLAH-PEAGQGLGE 121
Query: 118 IFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT-KYTIVSPELCPDYAILD 176
+F P + LI +P+T+G GSEV+ +++ G+T K +VSP L PD A+LD
Sbjct: 122 VFGVGNARGP----RLPLIQVPTTAGTGSEVTPIAIVTTGETTKMGVVSPHLLPDLALLD 177
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSK-ASTPFSDALAVKAARIIFERLEDSV--NG 233
L +P V +G+D +VHAIE+Y SK P SD LA +A R++ LE +V G
Sbjct: 178 ADLTLGLPPAVTAATGIDAMVHAIESYTSKLKKNPLSDLLAREALRLLAANLERAVGDGG 237
Query: 234 DAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMER 291
D +AR+ + A +AG AF N + VHA+++ + PHGL NA+ LP V+ FN
Sbjct: 238 DREARQAMLLGACLAGQAFANAPVAAVHALAYPLGGHFHIPHGLSNALVLPEVIRFNAPA 297
Query: 292 AREKYDAMAREL---GLSSAD------ELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVE 341
A Y +A L L D +L++++ +L+ + G+P +L + +E L ++
Sbjct: 298 AAGLYAELAPLLLGDRLRPGDRDALTVQLIDELAQLSPRNGLPSRLRDAGVDEAMLPRL- 356
Query: 342 DMSRKTYEDPLVSFNPVEPSVEDIKNLYLRA 372
+ + L+ NP E D +Y A
Sbjct: 357 -AADAMLQQRLLVNNPREVGEADALAIYRAA 386
>pir||S28477 probable dehydrogenase (EC 1.1.1.-) rfbM [similarity] - Vibrio
cholerae >gi|48392|emb|CAA42143.1| (X59554) rfbM [Vibrio
cholerae] >gi|1586114|prf||2203284C rfbM gene [Vibrio
cholerae]
Length = 326
Score = 145 bits (361), Expect = 1e-33
Identities = 93/258 (36%), Positives = 144/258 (55%), Gaps = 11/258 (4%)
Query: 66 EPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKA 125
EPSVE + V+ ++E+F P+ +ALGGGS+ID AK +FY+ P L +S ++ F A
Sbjct: 71 EPSVEELSGVIGELEDFKPDYILALGGGSIIDGAKLAWLFYECPTL--ESELLYRPF--A 126
Query: 126 QPLPKLKTRLIAIPSTSGAGSEVSAASVIKKG--DTKYTIVSPELCPDYAILDPRLPEKM 183
P + + + AIP+T GAGSEVS+A+V+ ++K +V+ + PD ILDP L ++
Sbjct: 127 LPSLRGRAKFAAIPTTVGAGSEVSSAAVMLDSVTNSKKAVVTHDFLPDLVILDPDLVSEV 186
Query: 184 PREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLE--DSVNGDAKAREEV 241
P +V + + D L HAIE YVS P + A +A II+ + N ++ E+
Sbjct: 187 PPKVLKTTVADALSHAIEGYVSLIDNPLMNTFAEQAVSIIYRYRHQFSNDNWSSEMLSEL 246
Query: 242 HYAATMAGIAFLNGRLGLVHAMSHKSAWIG-PHGLVNAIFLPYVMEFNMER--AREKYDA 298
AA AG + +GL HA++H+ HGL N + +P V+ FN A KY+
Sbjct: 247 QQAAMFAGWVQNHCIVGLSHAIAHQLGTFNIGHGLANGLLMPAVINFNYREHTAATKYEQ 306
Query: 299 MARELGLSSADELLEKVK 316
+ + GL + + LLE +K
Sbjct: 307 LIYKSGLPNKEALLELLK 324
>gb|AAC25557.1| (AF013169) iron containing alcohol dehydrogenase [Pyrococcus
furiosus]
Length = 390
Score = 140 bits (349), Expect = 3e-32
Identities = 115/394 (29%), Positives = 197/394 (49%), Gaps = 36/394 (9%)
Query: 4 FLKTTILMGRGSLENVKRIISE----GERVLIFSSRSMDRLGFLSEVADYVEEAGG---V 56
+L T I+ G G LE + +++ + G+ +++ RS G L V + +++AG V
Sbjct: 7 YLPTEIVFGPGKLEMLPKLVKKHGLSGKALIVTGRRSTKETGVLYRVQELLKQAGVESIV 66
Query: 57 YETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSV 116
++ II P P +V+E + + + LGGGS ID AKA+ + + G +D V
Sbjct: 67 FDKII---PNPISTHVDEGAEIARKENVSFVVGLGGGSAIDSAKAIAMTAASGGKYWDYV 123
Query: 117 AIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYT--IVSPELCPDYAI 174
KK P ++AIP+T G G+E +VI +TK I L P ++I
Sbjct: 124 PAVGGGKK----PTGALPIVAIPTTHGTGTEADPYAVITNPETKEKQGIGYDVLFPKFSI 179
Query: 175 LDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG- 233
+DP L +P++ + +D H+IEA+++ + P+SD LA+ + R I L +
Sbjct: 180 VDPELMLTLPKDQTVYTSMDAFYHSIEAFLNVRANPYSDVLALDSMRRIVTYLPLAYENL 239
Query: 234 -DAKAREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWI---GPHGLVNAIFLPYVMEFNM 289
+ +AR ++ +A+T AGI + HA+ H + PHGL I PY+ E+ +
Sbjct: 240 RNLEARTQLAWASTEAGITETVTGVVANHALEHGLSGFYPEVPHGLGLCILGPYLFEYIL 299
Query: 290 ERAREKYDAMAREL-------GLSSADELLEKVKELNEKLGV-PKLSEI-VKEEEFLGKV 340
+ A EK + RE+ +A+ ++K+++ GV KL E+ VKEE+ +
Sbjct: 300 DYAYEKLAIVGREVFGVYEPNDRKAAELAIKKLRDFQSLFGVNKKLRELGVKEED----I 355
Query: 341 EDMSRKTYE--DPLVSFNPVEPSVEDIKNLYLRA 372
+M+R Y P++ P + VED++ +Y RA
Sbjct: 356 PEMARTAYRMMKPVIEATPGDLKVEDLEEIYRRA 389
>pir||A83503 probable iron-containing alcohol dehydrogenase PA1146 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9947067|gb|AAG04535.1|AE004544_7 (AE004544) probable
iron-containing alcohol dehydrogenase [Pseudomonas
aeruginosa]
Length = 393
Score = 135 bits (336), Expect = 9e-31
Identities = 108/335 (32%), Positives = 165/335 (49%), Gaps = 20/335 (5%)
Query: 7 TTILMGRGSLENVKR--IISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLP 64
T IL G G+LE + R ++ R L+ + M L L V + +E AG ++ L
Sbjct: 11 TDILCGVGALEQLPRRCALAGARRPLLVTDPGMRALEPLRLVRECLERAGIDHDLFHELS 70
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAP-GLD-FDSVAIFSRF 122
PS+ V+ + E + IALGGGS +D AK + + P GL+ F+ +
Sbjct: 71 SNPSLAEVQGGARRFEAGGHDALIALGGGSALDAAKGIALLSRDPHGLERFEWTQTLRSY 130
Query: 123 KKAQPLPKLKTR-LIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRL 179
P L L+A+P+T+G GSE+ +V+ K + EL LDPRL
Sbjct: 131 PTLADYPPLGLPPLLALPTTAGTGSELGREAVLTDTQLGIKRVVGHRELLAACVFLDPRL 190
Query: 180 PEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAKA 237
+P ++ +G+D L H +EA S P S +A++ R++ + LE++V D A
Sbjct: 191 TRGLPPALSAATGMDALTHHLEALFSPLYHPMSAGIALEGVRLVRQHLENAVRDGNDLAA 250
Query: 238 REEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIGP-----HGLVNAIFLPYVMEFNMERA 292
RE + A+ A +AF G LG VHA++H +G HGL+NA+ LPYV+ N
Sbjct: 251 REGMLVASASAAVAFQKG-LGGVHALAHP---LGARHHLHHGLLNAVLLPYVLLANRPAI 306
Query: 293 REKYDAMAR--ELGLSSADELLEKVKELNEKLGVP 325
+AR EL +S D LL + EL ++G+P
Sbjct: 307 EADAARLARYLELDEASFDGLLAWILELRARIGIP 341
>emb|CAA74334.1| (Y14015) alcohol dehydrogenase [Thermococcus hydrothermalis]
Length = 406
Score = 131 bits (327), Expect = 1e-29
Identities = 121/397 (30%), Positives = 189/397 (47%), Gaps = 41/397 (10%)
Query: 6 KTTILMGRGSLENVKRIISE------GERVLIFSSRSMDRLGFLSEVADYVEEAGGVYET 59
KTT G ++ I+ E + +L+ S + G V +EE G Y
Sbjct: 18 KTTNYFGLCAIHKFNDIVRELKGKGVDKVILVTGXSSYKKCGAWDVVRPALEENGVEYVH 77
Query: 60 IIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIF 119
+ P+V+ ++E EF + I +GGGS ID AK+V + + P + F
Sbjct: 78 YDKVGANPTVDMIDEAAEMGREFGAQAVIGIGGGSPIDSAKSVAILLEYPDKTARDLYEF 137
Query: 120 SRFKKAQPLPKLKTRLIAIPSTSGAGSEVS--AASVIKKGDTKYTIVSPELCPDYAILDP 177
RF + P +IAI +T G G+EV A + I + + K I + P YAI DP
Sbjct: 138 -RFTPVKAKP-----IIAINTTHGTGTEVDRFAVASIPEKEYKPAIAYDCIYPLYAIDDP 191
Query: 178 RLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVN--GDA 235
L K+P E +D L H EA +K + P+S LA +AAR+IF L +++N +
Sbjct: 192 ALTTKLPPEQTLYVTIDALNHITEAATTKVANPYSILLAKEAARLIFTYLPEALNHPDNL 251
Query: 236 KAREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG---PHGLVNAIFLPYVMEFNMERA 292
+AR + YA+ +AGI+F NG L HA+ H + + PHGL A+ LP V+
Sbjct: 252 QARYALLYASAIAGISFDNGLLHFTHALEHPLSAVKPDLPHGLGLAMLLPAVI------- 304
Query: 293 REKYDAMAREL------------GLSSADELL-EKVKELNEKLGV-PKLSEIVKEEEFLG 338
R+ Y A A+ L G+ EL+ +V+E +G+ KL+++ E +
Sbjct: 305 RQIYPATAKILAEVYRPLVPEAKGVPGEVELVARRVEEWLFSIGITEKLADVGFTEGDVD 364
Query: 339 KVEDMSRKTYE-DPLVSFNPVEPSVEDIKNLYLRALH 374
K+ ++ T D L+S PVE + E I +Y +L+
Sbjct: 365 KLTQLAMTTPSLDLLLSMAPVEATKERIAAIYRDSLY 401
>pir||F72416 alcohol dehydrogenase, iron-containing - Thermotoga maritima
(strain MSB8) >gi|4980602|gb|AAD35205.1|AE001697_5
(AE001697) alcohol dehydrogenase, iron-containing
[Thermotoga maritima]
Length = 387
Score = 127 bits (317), Expect = 2e-28
Identities = 107/386 (27%), Positives = 192/386 (49%), Gaps = 23/386 (5%)
Query: 3 FFLKTTILMGRGSLENVK-RIISEGERVLIFSSRSMDR-LGFLSEVADYVEEAGGVYETI 60
F+L T I+ G+++ ++ R G++ LI + RS + G L V D +++AG
Sbjct: 6 FYLPTEIIFRVGAVDELEERAKKLGKKALIVTGRSSTKKTGLLQRVVDLLKKAGVESFVF 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
+ P P ++V+E V + + I LGGGS ID AKA+ + G +D V +
Sbjct: 66 DKIVPNPISDHVDEAAEIVRKEKIDFIIGLGGGSPIDSAKAISITAPNEGKFWDYVPV-- 123
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPR 178
+P+ ++AIP+T G G+E +VI T K I P +++DP
Sbjct: 124 ---GGGKIPEKSIPVVAIPTTHGTGTEADPFAVITNPQTKEKVGIGYRNTFPVLSLVDPE 180
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV-NG-DAK 236
+ + +P++ + +D HAIEA+++ + P+SD LA+ A + I L + NG D +
Sbjct: 181 VMKTLPKDQTAYTSMDAFYHAIEAFLNVNANPYSDVLALDAMKRIVTYLPVAYENGEDME 240
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIGP---HGLVNAIFLPYVMEFNMERAR 293
AR + +A+T AGI + HA+ H + P HGL I PY+ E+ + A
Sbjct: 241 ARTNLAWASTEAGITETLTGVIANHALEHGLSGFYPEITHGLGLCITGPYLFEYIFDHAY 300
Query: 294 EKYDAMAREL-GLSSADE------LLEKVKELNEKLGVPK-LSEIVKEEEFLGKVEDMSR 345
E+ + RE+ G+ D+ ++K+++ E G+ K LSE+ ++E + K+ +
Sbjct: 301 ERLAIVGREVFGVYETDDRKAGRLAIKKLRDFQEMFGLNKRLSELGVKKEDIPKMAETGY 360
Query: 346 KTYEDPLVSFNPVEPSVEDIKNLYLR 371
+ + +V P + +D++ ++ R
Sbjct: 361 RIL-NGVVVVTPGNLTAKDMEEIFNR 385
>sp|P41795|EUTG_SALTY ETHANOLAMINE UTILIZATION PROTEIN EUTG >gi|687647|gb|AAA80211.1|
(U18560) eutG gene product [Salmonella typhimurium]
>gi|3885920|gb|AAC78120.1| (AF093749) alcohol
dehydrogenase [Salmonella typhimurium]
Length = 391
Score = 127 bits (316), Expect = 2e-28
Identities = 99/282 (35%), Positives = 144/282 (50%), Gaps = 20/282 (7%)
Query: 55 GVYETIIGLPP-EPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDF 113
GV T+ PP EP + +V + ++ E + + +A GGGSV+D AKAV + P
Sbjct: 77 GVAMTVWPCPPGEPCITDVCAAVAQLREAACDGVVAFGGGSVLDAAKAVALLVTNPDQTL 136
Query: 114 DSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPD 171
++ S + P LIA+P+T+G GSE + +VI K + L PD
Sbjct: 137 SAMTEHSTLRPRLP-------LIAVPTTAGTGSETTNVTVIIDAVSGRKQVLAHASLMPD 189
Query: 172 YAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFS-DALAVKAARIIFERLEDS 230
AILD + + +P V +G+D L HAIEAY A TP + A +I + L +
Sbjct: 190 VAILDAAVTD-VPPNVTAMTGIDALTHAIEAY--SALTPRRLPQPGIGAIAMIGKSLPKA 246
Query: 231 V--NGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVME 286
V D ARE + A+ MAG+AF + LGL HAM+H+ +A PHG NA+ LP VM
Sbjct: 247 VGYGHDLAARENMLLASCMAGMAFSSAGLGLCHAMAHQPGAALHIPHGQANAMLLPTVMG 306
Query: 287 FNMERAREKYDAMARELGLSSAD--ELLEKVKELNEKLGVPK 326
FN RE++ + R L +D + + V EL ++G K
Sbjct: 307 FNRMVCRERFSQIGRALTNKKSDDRDAIAAVCELIAEVGQSK 348
>pir||G70398 1,3 propanediol dehydrogenase - Aquifex aeolicus
>gi|2983604|gb|AAC07174.1| (AE000725) 1,3 propanediol
dehydrogenase [Aquifex aeolicus]
Length = 387
Score = 127 bits (315), Expect = 3e-28
Identities = 108/378 (28%), Positives = 181/378 (47%), Gaps = 21/378 (5%)
Query: 9 ILMGRGSLENVKRIISE-GERVLIFSSR-SMDRLGFLSEVADYVEEAGGVYETIIGLPPE 66
+ G G+++ + G + LI + R S G L ++ + +++ Y + P
Sbjct: 12 VFFGEGAVKKAGEVGRRFGFKALIVTGRKSTKESGALDKLINSLKQNQIEYVVFDEITPN 71
Query: 67 PSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQ 126
P+ + V + + + +GGGSV+D AKA+ + G +D V R+ +
Sbjct: 72 PTDKQVNKGAEIAVNEKVDFIVGIGGGSVLDAAKAISIVSSNEGYAWDYV----RYPEGS 127
Query: 127 PL-PKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELC--PDYAILDPRLPEKM 183
L P L +I IP+T+G GSEV+ SV+ TK +V P A++DP L M
Sbjct: 128 RLIPFLNRPVITIPTTAGTGSEVNRYSVLTNPMTKEKMVISHSLNYPKVALVDPELTYSM 187
Query: 184 PREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVN--GDAKAREEV 241
P + +G D L+HA+E+ +K ++ +VKA +I + L + + +AR +
Sbjct: 188 PPRLTALTGFDALMHALESLTNKRENFIAEEYSVKAIELIRKWLPVAFEEPENKEARRYM 247
Query: 242 HYAATMAGIAFLNGRLGLVHAMSHKSAWIGP---HGLVNAIFLPYVMEFNMERAREKYDA 298
YAA +AGIA + + L+HAM H + P H + PY+ FN EKY
Sbjct: 248 SYAAMLAGIAIDHLGVALIHAMEHPVSGHYPEVAHAEGLSALAPYITAFNYRGNPEKYAL 307
Query: 299 MARELG-LSSADELLEKVKELNEKLGVPK-LSEIVKEEEFLGKVEDMSRKTYEDPLVSF- 355
AR +G + ++ + + E+ +PK L E+ E+E K+ ++ Y SF
Sbjct: 308 FARLMGEEEKPHKAVDALVKFIERFSLPKTLKELGVEKE---KLPRLAEDVYMCARHSFA 364
Query: 356 -NPVEPSVEDIKNLYLRA 372
NPVE +E+++ LY RA
Sbjct: 365 VNPVEVGMEEVEELYERA 382
>gb|AAC44365.1| (U47861) GbsB [Bacillus subtilis]
Length = 393
Score = 126 bits (314), Expect = 3e-28
Identities = 107/388 (27%), Positives = 182/388 (46%), Gaps = 23/388 (5%)
Query: 3 FFLKTTILMGRGSLENV-KRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F + T I G G++++ + + + G + L+ + + + G V + ++EAG
Sbjct: 7 FEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADPVIESLKEAGIEVVLF 66
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
+ P P V V E ++ + +A+GGGS +D AKA+ V G D A
Sbjct: 67 NKVEPNPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAADG 126
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
+ +P L T IP+T+G GSEV+ +VI ++ + K+ P + I+DP
Sbjct: 127 KKPLENRIPPLTT----IPTTAGTGSEVTQWAVITDEEREFKFNTGGPLIAAHLTIIDPE 182
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAK 236
L MP V +G+D L HAIE Y K + P +DA+A+ A ++ + D +
Sbjct: 183 LHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYIKRAFADGEDLE 242
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIGP--HGLVNAIFLPYVMEFNMERARE 294
AR + AA +AG+++ + G HAMS I P HG A + VME+N + E
Sbjct: 243 ARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPE 302
Query: 295 KYDAMARELGLSSADELLEK--------VKELNEKLGVPKLSEIVKEEEFLGKVEDMSRK 346
K+ +A+ G+ ++ E+ + +L E L VP L E + +E +S++
Sbjct: 303 KFARIAKAFGIDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPDM---IERLSKE 359
Query: 347 TYEDPLVSFNPVEPSVEDIKNLYLRALH 374
+DP NP + + + +Y R +
Sbjct: 360 AMKDPQTFGNPRDLNEKAYNWIYKRCFN 387
>pir||B69629 alcohol dehydrogenase gbsB - Bacillus subtilis
>gi|2635589|emb|CAB15083.1| (Z99119) alcohol
dehydrogenase [Bacillus subtilis]
Length = 403
Score = 126 bits (314), Expect = 3e-28
Identities = 107/388 (27%), Positives = 182/388 (46%), Gaps = 23/388 (5%)
Query: 3 FFLKTTILMGRGSLENV-KRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F + T I G G++++ + + + G + L+ + + + G V + ++EAG
Sbjct: 17 FEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADPVIESLKEAGIEVVLF 76
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
+ P P V V E ++ + +A+GGGS +D AKA+ V G D A
Sbjct: 77 NKVEPNPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAADG 136
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
+ +P L T IP+T+G GSEV+ +VI ++ + K+ P + I+DP
Sbjct: 137 KKPLENRIPPLTT----IPTTAGTGSEVTQWAVITDEEREFKFNTGGPLIAAHLTIIDPE 192
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAK 236
L MP V +G+D L HAIE Y K + P +DA+A+ A ++ + D +
Sbjct: 193 LHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYIKRAFADGEDLE 252
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIGP--HGLVNAIFLPYVMEFNMERARE 294
AR + AA +AG+++ + G HAMS I P HG A + VME+N + E
Sbjct: 253 ARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPE 312
Query: 295 KYDAMARELGLSSADELLEK--------VKELNEKLGVPKLSEIVKEEEFLGKVEDMSRK 346
K+ +A+ G+ ++ E+ + +L E L VP L E + +E +S++
Sbjct: 313 KFARIAKAFGIDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPDM---IERLSKE 369
Query: 347 TYEDPLVSFNPVEPSVEDIKNLYLRALH 374
+DP NP + + + +Y R +
Sbjct: 370 AMKDPQTFGNPRDLNEKAYNWIYKRCFN 397
>sp|P71017|GBSB_BACSU ALCOHOL DEHYDROGENASE
Length = 402
Score = 126 bits (314), Expect = 3e-28
Identities = 107/388 (27%), Positives = 182/388 (46%), Gaps = 23/388 (5%)
Query: 3 FFLKTTILMGRGSLENV-KRIISEG-ERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
F + T I G G++++ + + + G + L+ + + + G V + ++EAG
Sbjct: 16 FEIPTVIKHGIGAIKHTGEEVAALGVSKALLVTDPGIYKAGVADPVIESLKEAGIEVVLF 75
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
+ P P V V E ++ + +A+GGGS +D AKA+ V G D A
Sbjct: 76 NKVEPNPPVRLVNEGSELYKKENCNGLVAVGGGSSMDTAKAIGVEATHEGSVLDYEAADG 135
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
+ +P L T IP+T+G GSEV+ +VI ++ + K+ P + I+DP
Sbjct: 136 KKPLENRIPPLTT----IPTTAGTGSEVTQWAVITDEEREFKFNTGGPLIAAHLTIIDPE 191
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAK 236
L MP V +G+D L HAIE Y K + P +DA+A+ A ++ + D +
Sbjct: 192 LHVSMPPHVTAMTGIDALAHAIECYTMKFAQPITDAVALMAIEYAAHYIKRAFADGEDLE 251
Query: 237 AREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIGP--HGLVNAIFLPYVMEFNMERARE 294
AR + AA +AG+++ + G HAMS I P HG A + VME+N + E
Sbjct: 252 ARYGMAQAAMLAGLSYGSESAGAAHAMSQTLGGIIPVAHGQCVAAMMGPVMEYNWKGYPE 311
Query: 295 KYDAMARELGLSSADELLEK--------VKELNEKLGVPKLSEIVKEEEFLGKVEDMSRK 346
K+ +A+ G+ ++ E+ + +L E L VP L E + +E +S++
Sbjct: 312 KFARIAKAFGIDTSKMTTEEAAKASVNWMYDLVEDLEVPTLEEQGVSPDM---IERLSKE 368
Query: 347 TYEDPLVSFNPVEPSVEDIKNLYLRALH 374
+DP NP + + + +Y R +
Sbjct: 369 AMKDPQTFGNPRDLNEKAYNWIYKRCFN 396
>gb|AAB63011.1| (U72646) alcohol dehydrogenase [Thermococcus sp.]
Length = 406
Score = 125 bits (312), Expect = 6e-28
Identities = 110/361 (30%), Positives = 178/361 (48%), Gaps = 21/361 (5%)
Query: 29 VLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPETFI 88
+L+ S S + G V +EE G Y + P+V+ ++E EF + I
Sbjct: 47 ILVTGSSSYKKCGAWDVVRPALEENGVEYVHYDKVGANPTVDMIDEAAEMGREFGAQAVI 106
Query: 89 ALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEV 148
+GGGS ID AK+V + + P + F RF + P +IAI +T GAG+EV
Sbjct: 107 GIGGGSPIDSAKSVAILLEYPDKTARDLYEF-RFTPVKAKP-----VIAINTTHGAGTEV 160
Query: 149 S--AASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSK 206
A + I + + K I + P YAI DP L K+ E +D L H EA +K
Sbjct: 161 DRFAVATIPEKEYKPAIAYDCIYPLYAIDDPSLTVKLSPEQTLYLTIDALNHVTEAATTK 220
Query: 207 ASTPFSDALAVKAARIIFERLEDSVN--GDAKAREEVHYAATMAGIAFLNGRLGLVHAMS 264
+ P+S LA +AAR+IF+ L +++ + +AR + YA+ +AGI+F NG L HA+
Sbjct: 221 LANPYSILLAKEAARLIFDYLPEALKHPDNLQARYALLYASAIAGISFDNGLLHFTHALE 280
Query: 265 HKSAWIG---PHGLVNAIFLPYVMEF----NMERAREKYDAMAREL-GLSSADELL-EKV 315
H + + PHG+ A+ LP V+ + E Y + E G+ EL+ +V
Sbjct: 281 HPLSAVKPDFPHGIGLAMLLPAVIRHIYPATAKILAEVYRPLVPEAKGVPGEVELVARRV 340
Query: 316 KELNEKLGV-PKLSEIVKEEEFLGKVEDMSRKTYE-DPLVSFNPVEPSVEDIKNLYLRAL 373
+E +G+ KL ++ E + ++ +++ KT + L+S P E + E I +Y +L
Sbjct: 341 EEWLFSIGITEKLEDVGFSETDVNRLTELAMKTPSLNLLLSMAPGEATRERIAAIYRDSL 400
Query: 374 H 374
+
Sbjct: 401 Y 401
>gb|AAD55928.1|AF148264_1 (AF148264) 4-hydroxybutyrate dehydrogenase GbD [uncultured
bacterium AH5]
Length = 378
Score = 125 bits (312), Expect = 6e-28
Identities = 101/358 (28%), Positives = 171/358 (47%), Gaps = 22/358 (6%)
Query: 28 RVLIFSSRSMDRLGFLSEVADYVEEA-GGVYETIIGLPPEPSVENVEEVLPKVEEFSPET 86
R L+ + + G L + D + + +++ P E +V E+ +
Sbjct: 31 RPLVITDAGVRAAGVLQKALDALADLPAAIFDGTPSNPTEAAVRAAREIW---NAHRADG 87
Query: 87 FIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGS 146
+A+GGGS ID+AK + + PG I K + +IA+P+TSG GS
Sbjct: 88 LVAVGGGSSIDLAKGLAILATHPGELKTYATIEGGSAK---ITAAVAPIIAVPTTSGTGS 144
Query: 147 EVSAASVIKKGD-TKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVS 205
EV+ +++ D K S L P AI DP L +P + +G+D + H +E ++S
Sbjct: 145 EVARGAIVIVDDHRKLGFHSWHLVPKGAICDPDLTLGLPAGLTAATGMDAVAHCMETFMS 204
Query: 206 KASTPFSDALAVKAARIIFERLEDSV-NG-DAKAREEVHYAATMAGIAFLNGRLGLVHAM 263
A P +D +A+ + +E + NG D +AR+++ A+ +AF G LG VH++
Sbjct: 205 AAFNPPADGIALDGLERAWAHIERATPNGSDREARKQMMSASMQGAMAFQKG-LGCVHSL 263
Query: 264 SHKSAWIGP---HGLVNAIFLPYVMEFNME----RAREKYDAMARELGLSSADELLEKVK 316
SH + P HG +NA+FLP V+ FN E + + + MA +GL+SA ++ E +
Sbjct: 264 SHALGGVNPKLHHGTLNAMFLPAVVRFNAEAESVKTERRLERMAHAMGLASAADIPEAIV 323
Query: 317 ELNEKLGVPK-LSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYLRAL 373
+N +LG+ L+ + E + KV + D NP S ED +++ A+
Sbjct: 324 AMNARLGLASGLAALGVERDCFDKVIE---GALADHCHKTNPRLASAEDYRSMLEAAM 378
>sp|P38945|4HDB_CLOKL NAD-DEPENDENT 4-HYDROXYBUTYRATE DEHYDROGENASE (4HBD)
>gi|347073|gb|AAA92348.1| (L21902) NAD-dependent
4-hydroxybutyrate dehydrogenase [Clostridium kluyveri]
Length = 371
Score = 123 bits (305), Expect = 4e-27
Identities = 109/331 (32%), Positives = 161/331 (47%), Gaps = 26/331 (7%)
Query: 53 AGGVYETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLD 112
A V++ GL EPS E + ++ + + IA+GGGSVID+AK + + Y LD
Sbjct: 49 ADAVFQEKYGLG-EPSDEMINNIIKDIGDKQYNRIIAVGGGSVIDIAKILSLKYTDDSLD 107
Query: 113 FDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASV--IKKGDTKYTIVSPELCP 170
F+ PL K K LI +P+T G GSEV+ SV +K+ TK I S EL
Sbjct: 108 L--------FEGKVPLVKNK-ELIIVPTTCGTGSEVTNVSVAELKRRHTKKGIASDELYA 158
Query: 171 DYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARII----FER 226
YA+L P + +P + S +D L+HA EAYVS + P++D +VKA +I +
Sbjct: 159 TYAVLVPEFIKGLPYKFFVTSSVDALIHATEAYVSPNANPYTDMFSVKAMELILNGYMQM 218
Query: 227 LEDSVNGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYV 284
+E + + E+ + AGIAF N +G VHA+S+ + PHG N +F +
Sbjct: 219 VEKGNDYRVEIIEDFVIGSNYAGIAFGNAGVGAVHALSYPIGGNYHVPHGEANYLFFTEI 278
Query: 285 MEFNMERAREKYDAMAREL--GLSSAD--ELLEKVKELNEKLGVPK-LSEIVKEEEFLGK 339
+ E+ +L G+ D E + + +L +KL K L E +EE +
Sbjct: 279 FKTYYEKNPNGKIKDVNKLLAGILKCDESEAYDSLSQLLDKLLSRKPLREYGMKEEEIET 338
Query: 340 VEDMSRKTYEDPLVSFNPVEP-SVEDIKNLY 369
D + + LV N EP S EDI N Y
Sbjct: 339 FADSVIEGQQRLLV--NNYEPFSREDIVNTY 367
>dbj|BAA22024.1| (AB002787) NAD+-dependent alcohol dehydrogenase [Entamoeba
histolytica]
Length = 172
Score = 116 bits (289), Expect = 3e-25
Identities = 57/136 (41%), Positives = 87/136 (63%), Gaps = 2/136 (1%)
Query: 160 KYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKA 219
KY + L P AI+DP +P+ ++GLDVLVHA EAYVS + ++D LA +A
Sbjct: 1 KYPLADYSLTPSVAIVDPMFTMSLPKRAIADTGLDVLVHATEAYVSVMANEYTDGLAREA 60
Query: 220 ARIIFERLEDSVNGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVN 277
+++FE L S NGD +ARE++H AAT+AG+ F + LG+ H+M+HK + + PHG
Sbjct: 61 VKLVFENLLKSYNGDLEAREKMHNAATIAGMGFASAFLGMDHSMAHKVGAGFHLPHGRCG 120
Query: 278 AIFLPYVMEFNMERAR 293
+ LP+V+ +N ++ R
Sbjct: 121 GVLLPHVIRYNGQKPR 136
>emb|CAC11961.1| (AL445065) NADPH-dependent butanol dehydrogenase related protein
[Thermoplasma acidophilum]
Length = 224
Score = 116 bits (287), Expect = 5e-25
Identities = 79/225 (35%), Positives = 118/225 (52%), Gaps = 11/225 (4%)
Query: 5 LKTTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLP 64
L I R ++N++ + E +L+ S R++ +L + + + ++ G Y I +
Sbjct: 10 LPRKIYQQRDIIDNIRETLQLKEPILLVSDRNIMKL-YGQRIKNALD--GSDYRVIEDVR 66
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKK 124
PEP V ++E + F P+T IA GGGSVID AK++ V P + V F
Sbjct: 67 PEPEVSDIENTYRSLSGFQPKTIIAFGGGSVIDFAKSIDVKISYPDRSLEEVNPF----- 121
Query: 125 AQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMP 184
+PL LK L+AIP+TSG GS+VS VI K + + +L P ++LD L P
Sbjct: 122 -EPL-NLKADLVAIPTTSGTGSDVSFGIVITDQGRKLALGNFDLVPYVSVLDSSLVPSDP 179
Query: 185 REVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLED 229
+ R +G+D LVH+ EA + S+ F+DALA KA IF L D
Sbjct: 180 -SIIRPTGVDALVHSFEALTANTSSIFTDALAEKAIETIFGILID 223
>gb|AAG01186.1|AF178965_1 (AF178965) long-chain primary alcohol dehydrogenase
[Thermoanaerobacter ethanolicus]
Length = 399
Score = 114 bits (282), Expect = 2e-24
Identities = 113/385 (29%), Positives = 182/385 (46%), Gaps = 27/385 (7%)
Query: 6 KTTILMGRGSLENVKRIISEGER------VLIFSSRSMDRLGFLSEVADYVEEAGGVYET 59
K T G GS++ +K I+ + +L+ S G V +E G Y
Sbjct: 17 KNTTYFGIGSIKKIKDILEVLKNKGINNVILVTGKGSYKASGAWDVVKPALETLGFKYSL 76
Query: 60 IIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIF 119
+ P P+V+ ++E E + I +GGGS ID AK+V V + D ++ ++
Sbjct: 77 YDKVGPNPTVDMIDEAAKIGRETGAKAVIGIGGGSPIDTAKSVAVLLEYT--DKNARELY 134
Query: 120 SRFKKAQPLPKLKTRLIAIPSTSGAGSEVS--AASVIKKGDTKYTIVSPELCPDYAILDP 177
+ + +P+ +IAI T G G+EV A + I + + K I L P YAI DP
Sbjct: 135 EQ----KFIPEKAAPIIAINLTHGTGTEVDRFAVATIPEKNYKPAIAYDCLYPMYAIDDP 190
Query: 178 RLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG--DA 235
L K+ ++ +D L H EA + ++P+S +A + R+I L +VN +
Sbjct: 191 SLMTKLDKKQTIAVTIDALNHVTEAATTLVASPYSVLMAKETVRLIVRYLPAAVNDPENL 250
Query: 236 KAREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIGP---HGLVNAIFLPYVME----FN 288
AR + YA+ +AGI+F NG L L HA+ H + + P HGL LP V++
Sbjct: 251 VARYYLLYASALAGISFDNGLLHLTHALEHPLSAVKPEIAHGLGLGAILPAVVKAIYPSV 310
Query: 289 MERAREKYDAMAREL-GL-SSADELLEKVKELNEKLG-VPKLSEIVKEEEFLGKVEDMSR 345
E E Y + L GL + A+ + +KV+E K+G KLS+ +E + + +++
Sbjct: 311 AEVLAEVYSPIVPGLKGLPAEAEYVAKKVEEWLFKVGCTQKLSDFGFTKEDIPTLVRLAK 370
Query: 346 KTYE-DPLVSFNPVEPSVEDIKNLY 369
T D L+S PVE + I +Y
Sbjct: 371 TTPSLDGLLSNAPVEATEAVIAKIY 395
>gb|AAG23616.1|AF244667_1 (AF244667) alcohol dehydrogenase [Carboxydothermus
hydrogenoformans]
Length = 204
Score = 114 bits (282), Expect = 2e-24
Identities = 75/187 (40%), Positives = 111/187 (59%), Gaps = 15/187 (8%)
Query: 86 TFIALGGGSVIDVAKAVKVFYDAPGL--DFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSG 143
T I+ GGGS D AK + + G D++ V K A+P+ L+A+ +T+G
Sbjct: 3 TGISFGGGSPHDCAKGIGLVATNGGSIKDYEGVN-----KSAKPM----LPLVAVNTTAG 53
Query: 144 AGSEVSAASVIKKGD--TKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIE 201
SE++ S+I D K IV + P A+ DP L +MP+ + +G+D L HAIE
Sbjct: 54 TASEMTKFSIIXDEDRHVKMAIVDWHVTPIMAVNDPELMVEMPKALTAATGMDALTHAIE 113
Query: 202 AYVSKASTPFSDALAVKAARIIFERLEDSV-NG-DAKAREEVHYAATMAGIAFLNGRLGL 259
AYVS +TP +DA A+KA +IF+ L+ +V NG D +AR+++ YA +AG+AF N LG
Sbjct: 114 AYVSIDATPVTDAAALKAIELIFKYLKRAVQNGKDIEARDKMAYAEYLAGVAFNNASLGY 173
Query: 260 VHAMSHK 266
VHAM+H+
Sbjct: 174 VHAMAHQ 180
>gb|AAF21473.1|U89273_1 (U89273) methanol:NDMA oxidoreductase [Amycolatopsis methanolica]
Length = 429
Score = 110 bits (273), Expect = 2e-23
Identities = 93/328 (28%), Positives = 152/328 (45%), Gaps = 29/328 (8%)
Query: 67 PSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQ 126
P NV + + +E ++F+++GGGS D K ++ G + + F++ + +
Sbjct: 80 PKDYNVMDAVKLYQENKCDSFVSIGGGSSHDACKGARISVAHDGRNVNDFEGFNKSENPR 139
Query: 127 PLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT------KYTIVSPELCPDYAILDPRLP 180
P IA+ +T+G GSE S A VI T KY AI DP L
Sbjct: 140 NPPH-----IAVSTTAGTGSETSWAYVITDTTTDPDNPHKYVAFDDASVATLAIDDPVLY 194
Query: 181 EKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAKAR 238
P + G DVL HA E YVS+ + S A++A ++ E L + + R
Sbjct: 195 YSCPIDYTAQCGFDVLAHASEPYVSRLNFEPSLGNALRAIKLTAENLRQATWNPSELSGR 254
Query: 239 EEVHYAATMAGIAFLNGRLGLVHAMSH--KSAWIGPHGLVNAIFLPYVMEFNMERAREKY 296
E + YA +A AF +G LG++H++SH + + HGL NAI LP V FNM A +++
Sbjct: 255 EGMMYAQYIAAQAFNSGGLGIIHSISHAVSAFYDTHHGLNNAIALPRVWAFNMPVAYKRF 314
Query: 297 DAMARELG--------LSSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKT 347
MA +G + +AD L ++ L + +G+P K +++ ++ ++ K
Sbjct: 315 ADMAEAMGVDTHGMTDVQAADALAAAIRLLRD-VGIPEKFTDVTQDSYSKNRLGQGPTKF 373
Query: 348 YEDPLVSFNPVEPSVEDIKNLYLRALHD 375
YE V ++ ED+ + L D
Sbjct: 374 YEQASV----IKGDDEDVDRITNHVLGD 397
>pir||I39568 4-hydroxybutyrate dehydrogenase (EC 1.1.1.61) [imported] -
Alcaligenes eutrophus >gi|695279|gb|AAC41425.1| (L36817)
NAD-dependent 4-hydroxybutyrate dehydrogenase [Ralstonia
eutropha] >gi|1093466|prf||2104199G succinic
semialdehyde dehydrogenase [Ralstonia eutropha]
Length = 382
Score = 108 bits (267), Expect = 1e-22
Identities = 91/330 (27%), Positives = 147/330 (43%), Gaps = 42/330 (12%)
Query: 64 PPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPG-------LDFDSV 116
P P+ V + + E + +A+GGGS ID+AK + + G ++ S
Sbjct: 65 PSNPTEAMVRKAAAQYREAGCDGLVAVGGGSSIDLAKGIAILATHEGELTTYATIEGGSA 124
Query: 117 AIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGD-TKYTIVSPELCPDYAIL 175
I KA PL IA+P+TSG GSEV+ ++I D K S L P A+
Sbjct: 125 RITD---KAAPL-------IAVPTTSGTGSEVARGAIIILDDGRKLGFHSWHLLPKSAVC 174
Query: 176 DPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNG-- 233
DP L +P + +G+D + H IE +++ A P +D +A+ + +E +
Sbjct: 175 DPELTLGLPAGLTAATGMDAIAHCIETFLAPAFNPPADGIALDGLERGWGHIERATRDGQ 234
Query: 234 DAKAREEVHYAATMAGIAFLNGRLGLVHAMSHKSAWIG-------PHGLVNAIFLPYVME 286
D AR + A+ +AF G LG VH++SH + HG +NA+ +P V+
Sbjct: 235 DRDARLNMMSASMQGAMAFQKG-LGCVHSLSHPLGGLKIDGRTGLHHGTLNAVVMPAVLR 293
Query: 287 FNMERAR----EKYDAMARELGLSSADELLEKVKELNEKLGVPKLSEIVKEEEFLGKVED 342
FN + ++Y + R + L ++ + V ++ +LG+P +G ED
Sbjct: 294 FNADAPTVVRDDRYARLRRAMHLPDGADIAQAVHDMTVRLGLP------TGLRQMGVTED 347
Query: 343 MSRKTYEDPLVSF----NPVEPSVEDIKNL 368
M K LV NP E S D + +
Sbjct: 348 MFDKVIAGALVDHCHKTNPKEASAADYRRM 377
>gb|AAB80771.1| (U21071) ThcE [Rhodococcus erythropolis]
Length = 424
Score = 107 bits (265), Expect = 2e-22
Identities = 95/326 (29%), Positives = 147/326 (44%), Gaps = 24/326 (7%)
Query: 41 GFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAK 100
G + E+ +E G + P NV E ++ ++ I++GGGS D AK
Sbjct: 55 GIIEELVGKIEYQGVEVVLYDKVESNPKDYNVMEAAALYQKEKCDSIISIGGGSSHDAAK 114
Query: 101 AVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIK----- 155
+V G + + F++ + P IA+ +T+G GSE S A VI
Sbjct: 115 GARVVIAHDGRNINEFEGFAKSTNKENPPH-----IAVSTTAGTGSETSWAYVITDTSDM 169
Query: 156 KGDTKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDAL 215
K+ AI DP L P+ G DVL H E +VS+ S
Sbjct: 170 NNPHKWVGFDEATIVTLAIDDPLLYYTCPQHFTAYCGFDVLAHGSEPFVSRLDFAPSLGN 229
Query: 216 AVKAARIIFERLEDSV--NGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK-SAWIGP 272
A+ + ++ + L ++V + KARE + A +AG AF +G LG+VH++SH SA+
Sbjct: 230 AIYSVELVAKNLREAVFEPRNLKAREGMMNAQYIAGQAFNSGGLGIVHSISHAVSAFFDS 289
Query: 273 H-GLVNAIFLPYVMEFNMERAREKYDAMARELG--------LSSADELLEKVKELNEKLG 323
H GL NAI LP V E+N+ E+Y +A LG + +AD +E L + +G
Sbjct: 290 HHGLNNAIALPRVWEYNLPSRYERYAQLAGALGVDTRNLTTVQAADAAVEAAIRLAKDVG 349
Query: 324 VPKLSEIVKEEEFLGKVEDMSRKTYE 349
+P V+ + + M+ K YE
Sbjct: 350 IPDNFGQVRTDSY--AKNQMNTKKYE 373
>sp|O05239|ADHA_BACSU PROBABLE NADH-DEPENDENT BUTANOL DEHYDROGENASE 1
>gi|7431291|pir||A70011 NADH-dependent butanol
dehydrogenase homolog yugJ - Bacillus subtilis
>gi|1934793|emb|CAB07922.1| (Z93934) NADH-dependent
butanol dehydrogenase [Bacillus subtilis]
>gi|2635633|emb|CAB15126.1| (Z99120) similar to
NADH-dependent butanol dehydrogenase [Bacillus subtilis]
Length = 387
Score = 101 bits (250), Expect = 1e-20
Identities = 100/362 (27%), Positives = 163/362 (44%), Gaps = 37/362 (10%)
Query: 7 TTILMGRGSLENVKRIISE-GERVL-IFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLP 64
T ++ GRG +E + + G+ VL ++ S+ R G +V + + +AG + G+
Sbjct: 10 TKLIFGRGEVERLPEELKPYGKNVLLVYGGGSIKRSGLYDQVIEQLNKAGATVHELAGVE 69
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKK 124
P P V V + + +E + + +A+GGGSVID KA+ A G +D A KK
Sbjct: 70 PNPRVSTVNKGVAICKEQNIDFLLAVGGGSVIDCTKAI-----AAGAKYDGDAWDIVTKK 124
Query: 125 AQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPEK 182
QP L + + + GSE+++ SVI +T KY SP + P ++ILDP
Sbjct: 125 HQPKDALPFGTVL--TLAATGSEMNSGSVITNWETKEKYGWGSPLVFPKFSILDPVNTFT 182
Query: 183 MPREVARNSGLDVLVHAIEAYVSKAS-TPFSDALAVKAARIIFERLEDSVNG--DAKARE 239
+P+ +D++ H E Y S TP+ D + R + E +N + + RE
Sbjct: 183 VPKNHTIYGMVDMMSHVFEQYFHHVSNTPYQDRMCESLLRTVIETAPKLINDLENYELRE 242
Query: 240 EVHYAATMAGIAFLNGRLGL-------VHAMSH--KSAWIGPHGLVNAIFLPYVMEFNME 290
+ Y T+A LNG L + H + H + + PH AI P M +
Sbjct: 243 TILYTGTIA----LNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMRHTLS 298
Query: 291 R--AREKYDAM----ARELGLSSADELLEKVKELN---EKLGVP-KLSEIVKEEEFLGKV 340
AR K A+ E G + + LE + +L+ LG P +L++ +E L +
Sbjct: 299 ENPARMKQLAVRVFDVEEAGKTDEEIALEGIDKLSAFWTSLGAPNRLADYDINDEQLDTI 358
Query: 341 ED 342
D
Sbjct: 359 AD 360
>pir||T26686 hypothetical protein Y38F1A.6 - Caenorhabditis elegans
>gi|5824752|emb|CAA21631.2| (AL032639) predicted using
Genefinder~contains similarity to Pfam domain: PF00465
(Iron-containing alcohol dehydrogenases), Score=177.7,
E-value=1.9e-50, N=2~cDNA EST EMBL:Z14517 comes from
this gene; cDNA EST yk18d4.3 comes from this gene~cDNA
EST yk1>
Length = 465
Score = 101 bits (250), Expect = 1e-20
Identities = 97/365 (26%), Positives = 160/365 (43%), Gaps = 51/365 (13%)
Query: 57 YETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSV 116
YE + EP+V ++++ + + ++FIA+GGGSVID KA ++ P DF
Sbjct: 101 YEVFDDVLIEPTVNSMQKAIAFAKSKQFDSFIAVGGGSVIDTTKAAALYASNPEADFLDF 160
Query: 117 AIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELC--PDYAI 174
+ F K+ LIA+P+T+G GSE +AA+++ + K C P A+
Sbjct: 161 -VGPPFGKSMQPKNPMLPLIAVPTTAGTGSETTAAAIMDLPEHKCKTGIRLRCIKPYLAV 219
Query: 175 LDPRLPEKMPREVARNSGLDVLVHAIEAYVS-------------------KASTPFSDAL 215
+DP MPR VA SG DVL HA+E++ + + S P SD
Sbjct: 220 VDPLNVMSMPRNVAIYSGFDVLCHALESFTALPFDQRSPRPENPGVRPLYQGSNPISDVW 279
Query: 216 AVKAARIIFERLEDSV--NGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSH-------- 265
+ +A RII + S+ D +AR E+ A++ AGI F N + L H +S+
Sbjct: 280 SKEALRIIGKYFRRSIFDPTDEEARTEMLKASSFAGIGFGNAGVHLCHGLSYPISSQAKS 339
Query: 266 -------KSAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELGLSSADE-------- 310
K + PHGL EF +++ A+ LG +
Sbjct: 340 CVADDYPKEKNLIPHGLSVMTTAVADFEFTTAACPDRHLISAQTLGADIPNNASNEYISR 399
Query: 311 -LLEKVKELNEKLGVPKLSEIVKEEEFLGKVEDMSR-KTYEDPLVSFNPVEPSVEDIKNL 368
L ++++ GVP + + E +E ++ ++ P ++ +P E I L
Sbjct: 400 TLCDRLRGYMRDFGVPNGLKGMGFE--FSDIEMLTEAASHSVPNIAISPKSADREIISTL 457
Query: 369 YLRAL 373
Y ++L
Sbjct: 458 YEKSL 462
>emb|CAB45024.1| (AL078635) putative alcohol dehydrogenase [Amycolatopsis
orientalis]
Length = 377
Score = 100 bits (246), Expect = 3e-20
Identities = 81/303 (26%), Positives = 142/303 (46%), Gaps = 17/303 (5%)
Query: 6 KTTILMGRGSLENVKRIISE--GERVLIFSSRS-MDRLGFLSEVADYVEEAGGVYETIIG 62
+ ++ G G + ++ + +RVL+ ++R+ + R G +A + ++
Sbjct: 9 RVEVIEGAGVAAKLPAVLRKMRAQRVLVVATRNAVHRTGAPGWLARHA------HDYFYD 62
Query: 63 LPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRF 122
+ P P +V V + P+ I +GGGS +D AKAV++ +
Sbjct: 63 VAPNPQAADVVRGARAVLRYKPDVVIGVGGGSTLDTAKAVRLLPPTKDEAERVLRGDEPI 122
Query: 123 KKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEK 182
+ A+P RL+ IP+T+G GSEV+A + I G KY++ P + D A++DP L
Sbjct: 123 RVARP-----PRLVVIPTTAGPGSEVTAFATIYVGTRKYSLDHPTVKADVALIDPALTHS 177
Query: 183 MPREVARNSGLDVLVHAIEAYVS-KASTPFSDALAVKAARIIFERLEDSVNGDAKAREEV 241
P + + LD HAIE++ S ++S P D AR++ + + ++ RE +
Sbjct: 178 CPPTLTAHCALDATAHAIESWWSLRSSAPSRDLARSALARLVPILQKGFRDTSSQQRELL 237
Query: 242 HYAATMAGIAFLNGRLGLVHAMSH-KSAWIG-PHGLVNAIFLPYVMEFNMERAREKYDAM 299
AA MAG A R HA ++ +A G PHGL A+ L +++ + + M
Sbjct: 238 SNAAVMAGQAIDLTRTTAAHAFAYPTTARFGVPHGLACALHLVWLLPLTLSQLEPSSSRM 297
Query: 300 ARE 302
A +
Sbjct: 298 AAD 300
>pir||F82428 iron-containing alcohol dehydrogenase VCA0702 [imported] - Vibrio
cholerae (group O1 strain N16961)
>gi|9658120|gb|AAF96601.1| (AE004399) iron-containing
alcohol dehydrogenase [Vibrio cholerae]
Length = 382
Score = 100 bits (246), Expect = 3e-20
Identities = 90/325 (27%), Positives = 154/325 (46%), Gaps = 32/325 (9%)
Query: 7 TTILMGRGSLENVKRIISEGERVLI-FSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPP 65
T I G+G + +++R I + +VL+ + S+ R G +V + + E V G+ P
Sbjct: 9 TMIHFGQGQIASIRRDIPKDHKVLVLYGGGSIKRNGVYDQVVEALSEHQWV--EFSGVEP 66
Query: 66 EPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAK--AVKVFYDAPGLDFDSVAIFSRFK 123
P+ E +++ + V+ + +A+GGGSVID +K A FY+ G +D + K
Sbjct: 67 NPTKETLDKAVHIVKNQHIDFILAVGGGSVIDGSKYVAAAAFYEGDG--WDILTGQHTVK 124
Query: 124 KAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRLPE 181
+A P+ + T +P+T GSE + +VI K +T K +SP + P +A+LDP + +
Sbjct: 125 QATPIGAILT----LPAT---GSESNTGAVITKAETQDKLAFLSPAVQPRFAVLDPDVMK 177
Query: 182 KMPREVARNSGLDVLVHAIEAYVS-KASTPFSDALAVKAARIIFERLEDSVNGDAKA-RE 239
+P N +D VH E Y++ + A R + D VN D A R
Sbjct: 178 SLPERQLVNGLVDAWVHVCEQYLTLPTQAMVQEGYAEVLLRNLLALGADFVNRDNDAWRA 237
Query: 240 EVHYAATMAGIAFLNGRLG-------LVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNME 290
+ + A A LNG +G H + H+ + W H AI P+++ ++
Sbjct: 238 NLMWTANQA----LNGLIGTGVPQDWATHMIGHELTALWHVDHARSLAIVQPWLLRNQLK 293
Query: 291 RAREKYDAMAREL-GLSSADELLEK 314
+ K + M + + GL +D+L EK
Sbjct: 294 HKKAKLEQMGKNVFGLPQSDDLAEK 318
>pir||S60213 fomC protein - Streptomyces wedmorensis >gi|1061009|dbj|BAA32497.1|
(AB016934) fomC [Streptomyces wedmorensis]
Length = 262
Score = 97.9 bits (240), Expect = 2e-19
Identities = 59/186 (31%), Positives = 91/186 (48%), Gaps = 6/186 (3%)
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKK 124
P P+V++V L V E P+ + +GGGS +D+AK + V P D + R +
Sbjct: 65 PNPAVDDVARGLDAVRELRPDAVVGVGGGSAMDMAKLLCVLDGVPRADLED-----RVRT 119
Query: 125 AQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMP 184
+ +L+ P+TSG+GSE + +V+ GD KY++ L PD A+LDP L E
Sbjct: 120 GPAADTRRMKLVLAPTTSGSGSEATQFAVVYVGDAKYSVAGAGLLPDAAVLDPALTESGT 179
Query: 185 REVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREEV-HY 243
SG+D + IE+ + S LA + R++ +ED V G A +
Sbjct: 180 PHQRATSGVDAIAQGIESLWAVGGDDESRGLARRGLRLLLAGIEDFVAGRTPASPAPWRW 239
Query: 244 AATMAG 249
AAT +G
Sbjct: 240 AATSSG 245
>sp|O05240|ADHB_BACSU PROBABLE NADH-DEPENDENT BUTANOL DEHYDROGENASE 2
>gi|7431290|pir||B70011 NADH-dependent butanol
dehydrogenase homolog yugK - Bacillus subtilis
>gi|1934794|emb|CAB07923.1| (Z93934) NADH-dependent
butanol dehydrogenase [Bacillus subtilis]
>gi|2635632|emb|CAB15125.1| (Z99120) similar to
NADH-dependent butanol dehydrogenase [Bacillus subtilis]
Length = 390
Score = 97.9 bits (240), Expect = 2e-19
Identities = 101/377 (26%), Positives = 169/377 (44%), Gaps = 45/377 (11%)
Query: 3 FFLKTTILMGRGSLENVKRIISE-GERVL-IFSSRSMDRLGFLSEVADYVEEAGGVYETI 60
++ T ++ G+G LE +++ G+ VL ++ S+ R G +V ++E G V +
Sbjct: 6 YYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGAVVHEL 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVA--I 118
G+ P P + VE+ + E + +A+GGGSVID KA+ A G+ +D A I
Sbjct: 66 SGVEPNPRLATVEKGIGLCREHDIDFLLAVGGGSVIDCTKAI-----AAGVKYDGDAWDI 120
Query: 119 FSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILD 176
FS+ A+ T L + + GSE++ SVI +T K+ S P ++ILD
Sbjct: 121 FSKKVTAEDALPFGTVL----TLAATGSEMNPDSVITNWETNEKFVWGSNVTHPRFSILD 176
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKA-STPFSDALAVKAARIIFER----LEDSV 231
P +P +D++ H E Y +TP D + + + E LED
Sbjct: 177 PENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLE 236
Query: 232 NGDAKAREEVHYAATMAGIAFLNGRLGL-------VHAMSH--KSAWIGPHGLVNAIFLP 282
N + RE + YA T+A LNG L + H M H + + PH AI P
Sbjct: 237 N--YELRETILYAGTIA----LNGTLQMGYFGDWASHTMEHAVSAVYDIPHAGGLAILFP 290
Query: 283 YVMEFNMERAREKYDAMAREL------GLSSADELLEKVKELN---EKLGVP-KLSEIVK 332
M + ++ ++ + + G + + LE + +L+ LG P +L++
Sbjct: 291 NWMRYTLDTNVGRFKNLMLNMFDIDTEGKTDKEIALEGIDKLSAFWTSLGAPSRLADYNI 350
Query: 333 EEEFLGKVEDMSRKTYE 349
EE L + D++ K E
Sbjct: 351 GEEKLELIADIAAKEME 367
>pir||G72329 hypothetical protein TM0820 - Thermotoga maritima (strain MSB8)
>gi|4981351|gb|AAD35902.1|AE001749_5 (AE001749)
NADH-dependent butanol dehydrogenase, putative
[Thermotoga maritima]
Length = 395
Score = 97.5 bits (239), Expect = 2e-19
Identities = 93/364 (25%), Positives = 167/364 (45%), Gaps = 35/364 (9%)
Query: 3 FFLKTTILMGRGSLENV-KRIISEGERVLIF--SSRSMDRLGFLSEVADYVEEAGGVYET 59
F T I+ GRG++ + + I + G R ++F S+ + G +V D +++ G +
Sbjct: 6 FHNPTKIVFGRGTIPKIGEEIKNAGIRKVLFLYGGGSIKKNGVYDQVVDSLKKHGIEWVE 65
Query: 60 IIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIF 119
+ G+ P P + V E + ++ E + +GGGSV+D AKAV G +D+
Sbjct: 66 VSGVKPNPVLSKVHEAVEVAKKEKVEAVLGVGGGSVVDSAKAVAAGALYEGDIWDAFIGK 125
Query: 120 SRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDP 177
+ +KA P + + + S G+E++ +VI +K KY + S L P +I+DP
Sbjct: 126 YQIEKALP-------IFDVLTISATGTEMNGNAVITNEKTKEKYGVSSKALYPKVSIIDP 178
Query: 178 RLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVN--GDA 235
+ +P+E +D + H +E Y +S S+ +A R I + E + D
Sbjct: 179 SVQFTLPKEQTVYGAVDAISHILEYYFDGSSPEISNEIAEGTIRTIMKMTERLIEKPDDY 238
Query: 236 KAREEVHYAATMAGIAFLNGRLGL--------VHAMSH--KSAWIGPHGLVNAIFLPYVM 285
+AR + ++AT+A LNG + + H + H + + HG AI P M
Sbjct: 239 EARANLAWSATIA----LNGTMAVGRRGGEWACHRIEHSLSALYDIAHGAGLAIVFPAWM 294
Query: 286 EFNMERAREKYDAMAREL-GLSSADELL-----EKVKELNEKLGVP-KLSEIVKEEEFLG 338
++ + +++ A+++ G E L E K +K+G P L + EE +
Sbjct: 295 KYVYRKNPAQFERFAKKIFGFEGEGEELILKGIEAFKNWLKKVGAPVSLKDAGIPEEDID 354
Query: 339 KVED 342
K+ D
Sbjct: 355 KIVD 358
>emb|CAC11962.1| (AL445065) NADPH-dependent butanol dehydrogenase related protein
[Thermoplasma acidophilum]
Length = 148
Score = 95.2 bits (233), Expect = 1e-18
Identities = 56/151 (37%), Positives = 87/151 (57%), Gaps = 9/151 (5%)
Query: 231 VNGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFN 288
+ G+ ARE +H AATMAGI+F N L HA+ H S HG +FLPYV+EFN
Sbjct: 1 MKGEEDARENMHLAATMAGISFTNSGTALAHALGHSFGSTHHVVHGTCVGLFLPYVIEFN 60
Query: 289 M--ERAREKYDAMARELGLSSADELLEKVKELNEKLGVP-KLSEI-VKEEEFLGKVEDMS 344
E A EKY +AR LG A + +++L ++G P ++EI + ++ ++ ++ M
Sbjct: 61 SSDENASEKYARIARRLGYKDA---ISALRDLYRRIGQPLTVAEIGIPKDAYMKSLDSMV 117
Query: 345 RKTYEDPLVSFNPVEPSVEDIKNLYLRALHD 375
K D ++FNPV ED++++Y++A D
Sbjct: 118 EKALADSELAFNPVIAGDEDVRSIYIKAYGD 148
>pir||B47013 butanol dehydrogenase (EC 1.1.1.-) II - Clostridium acetobutylicum
Length = 390
Score = 94.4 bits (231), Expect = 2e-18
Identities = 98/386 (25%), Positives = 170/386 (43%), Gaps = 38/386 (9%)
Query: 3 FFLKTTILMGRGSLENVKRIISE-GERVLI-FSSRSMDRLGFLSEVADYVEEAGGVYETI 60
+ + T I G+ + + R + + G +VLI + S+ R G + +E+ + +
Sbjct: 6 YSIPTRIFFGKDKINVLGRELKKYGSKVLIVYGGGSIKRNGIYDKAVSILEKNSIKFYEL 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ P P V VE+ + E E +A+GGGS ID AK + + G +D V S
Sbjct: 66 AGVEPNPRVTTVEKGVKICRENGVEVVLAIGGGSAIDCAKVIAAACEYDGNPWDIVLDGS 125
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVS--PELCPDYAILDPR 178
+ K+ P+ + T + GSE+ +VI DT +++ P++ P ++ILDP
Sbjct: 126 KIKRVLPIASILT-------IAATGSEMDTWAVINNMDTNEKLIAAHPDMAPKFSILDPT 178
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPF-SDALAVKAAR--IIFERLEDSVNGDA 235
+P D++ H E Y S T + D +A R I + + + D
Sbjct: 179 YTYTVPTNQTAAGTADIMSHIFEVYFSNTKTAYLQDRMAEALLRTCIKYGGIALAKPDDY 238
Query: 236 KAREEVHYAATMAGIAFLNGRLGL-------VHAMSHK-SAWIG-PHGLVNAIFLPYVME 286
+AR + +A+++A +NG L VH M H+ SA+ HG+ AI P ME
Sbjct: 239 EARANLMWASSLA----INGLLTYGKDTNWSVHLMEHELSAYYDITHGVGLAILTPNWME 294
Query: 287 F--NMERAREKYDAMARELGLSS-------ADELLEKVKE-LNEKLGVP-KLSEIVKEEE 335
+ N + + + G+ A + ++K ++ LG+P +L ++ EEE
Sbjct: 295 YILNNDTVYKFVEYGVNVWGIDKEKNHYDIAHQAIQKTRDYFVNVLGLPSRLRDVGIEEE 354
Query: 336 FLGKVEDMSRKTYEDPLVSFNPVEPS 361
L + S K + + PV S
Sbjct: 355 KLDIMAKESVKLTGGTIGNLRPVNAS 380
>sp|Q04945|ADHB_CLOAB NADH-DEPENDENT BUTANOL DEHYDROGENASE B (BDH II)
>gi|144731|gb|AAA23207.1| (M96946) butanol dehydrogenase
[Clostridium acetobutylicum]
Length = 390
Score = 94.0 bits (230), Expect = 2e-18
Identities = 98/386 (25%), Positives = 169/386 (43%), Gaps = 38/386 (9%)
Query: 3 FFLKTTILMGRGSLENVKRIISE-GERVLI-FSSRSMDRLGFLSEVADYVEEAGGVYETI 60
+ + T I G+ + + R + + G +VLI + S+ R G + +E+ + +
Sbjct: 6 YSIPTRIFFGKDKINVLGRELKKYGSKVLIVYGGGSIKRNGIYDKAVSILEKNSIKFYEL 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS 120
G+ P P V VE+ + E E +A+GGGS ID AK + + G +D V S
Sbjct: 66 AGVEPNPRVTTVEKGVKICRENGVEVVLAIGGGSAIDCAKVIAAACEYDGNPWDIVLDGS 125
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVS--PELCPDYAILDPR 178
+ K+ P+ + T + GSE+ +VI DT +++ P++ P ++ILDP
Sbjct: 126 KIKRVLPIASILT-------IAATGSEMDTWAVINNMDTNEKLIAAHPDMAPKFSILDPT 178
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVSKASTPF-SDALAVKAAR--IIFERLEDSVNGDA 235
+P D++ H E Y S T + D +A R I + + D
Sbjct: 179 YTYTVPTNQTAAGTADIMSHIFEVYFSNTKTAYLQDRMAEALLRTCIKYGGIALEKPDDY 238
Query: 236 KAREEVHYAATMAGIAFLNGRLGL-------VHAMSHK-SAWIG-PHGLVNAIFLPYVME 286
+AR + +A+++A +NG L VH M H+ SA+ HG+ AI P ME
Sbjct: 239 EARANLMWASSLA----INGLLTYGKDTNWSVHLMEHELSAYYDITHGVGLAILTPNWME 294
Query: 287 F--NMERAREKYDAMARELGLSS-------ADELLEKVKE-LNEKLGVP-KLSEIVKEEE 335
+ N + + + G+ A + ++K ++ LG+P +L ++ EEE
Sbjct: 295 YILNNDTVYKFVEYGVNVWGIDKEKNHYDIAHQAIQKTRDYFVNVLGLPSRLRDVGIEEE 354
Query: 336 FLGKVEDMSRKTYEDPLVSFNPVEPS 361
L + S K + + PV S
Sbjct: 355 KLDIMAKESVKLTGGTIGNLRPVNAS 380
>gb|AAF46832.1| (AE003457) T3dh gene product [Drosophila melanogaster]
Length = 464
Score = 92.8 bits (227), Expect = 5e-18
Identities = 82/315 (26%), Positives = 136/315 (43%), Gaps = 39/315 (12%)
Query: 28 RVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPETF 87
+V + + +++ +L + D + G YE EP+ ++ + + F
Sbjct: 72 KVCLVTDKNVVQLPSVKVALDSLARNGINYEVYDETRVEPTDGSMWHAVEFARGKEFDAF 131
Query: 88 IALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSE 147
+A+GGGS +D AKA +F +F + + KLK LIA+P+TSG GSE
Sbjct: 132 LAIGGGSAMDTAKAANLFSSDANAEFLDYVNCPIGRGKEISVKLKP-LIAMPTTSGTGSE 190
Query: 148 VSAASVI--KKGDTKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVS 205
+ ++ KK K I S L P A++DP P+ V +G DV HA+E++ +
Sbjct: 191 TTGVAIFDYKKLHAKTGISSKFLKPTLAVIDPLHTLSQPQRVMAFAGFDVFCHALESFTA 250
Query: 206 -------------------KASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEVHYA 244
+ P SD A A I + +++ + +AR ++H A
Sbjct: 251 VDYRERGLAPSDPSLRPTYQGRNPVSDVWARFALETIRKNFVNAIYQPDNLEARSQMHLA 310
Query: 245 ATMAGIAFLNGRLGLVHAMSHKSAW---------------IGPHGLVNAIFLPYVMEFNM 289
+TMAG+ F N + L H +S+ + + PHGL I P V EF
Sbjct: 311 STMAGVGFGNAGVHLCHGLSYPISGNVRDYKPKGYSADHALIPHGLSVVISAPAVFEFTA 370
Query: 290 ERAREKYDAMARELG 304
+++ A+ LG
Sbjct: 371 PACPDRHLEAAQLLG 385
>dbj|BAB07063.1| (AP001518) NADH-dependent butanol dehydrogenase [Bacillus
halodurans]
Length = 387
Score = 89.7 bits (219), Expect = 5e-17
Identities = 89/369 (24%), Positives = 171/369 (46%), Gaps = 39/369 (10%)
Query: 29 VLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPETFI 88
+L++ S+ R G V + +E AG + G+ P P + V++ + + + E +
Sbjct: 34 LLVYGGGSIKRNGLYDRVLEQLERAGKAVIELSGVEPNPRLTTVQKGIDLCKAQNIEFIL 93
Query: 89 ALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEV 148
A+GGGSVID KA+ G +D + + + A P + T + GSE+
Sbjct: 94 AVGGGSVIDATKAIAAGAKYDGDVWDIITKKTTAEDALPFGTILT-------LAATGSEM 146
Query: 149 SAASVIKKGDTKYTI--VSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSK 206
+A SVI +T I S + P ++ILDP+ +P++ +D++ H +E+Y
Sbjct: 147 NANSVITNWETNQKIGWSSSHVYPMFSILDPKNTVTVPKDQTIYGIVDMMSHVLESYFHP 206
Query: 207 AS-TPFSDALAVKAARIIFERLEDSVNG--DAKAREEVHYAATMAGIAFLNGRLGL---- 259
S TP D + + + E VN + RE + Y TMA LNG L +
Sbjct: 207 VSNTPLQDRMCEAVLQTVIETGPKLVNDLESLEYRETILYCGTMA----LNGVLSMGARG 262
Query: 260 ---VHAMSH--KSAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMA-RELGL-------- 305
H + H + + PH AI P+ + +++++ +++ +A R G+
Sbjct: 263 DWATHNIEHAVSAVYDIPHAGGLAILFPHWLTYSLDQKIDRHVQLATRVFGVDPSGKSEE 322
Query: 306 SSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVED 364
+ A E +E++ +LG P +L++ ++ + ++ +++ T + L +F ++ +D
Sbjct: 323 AIAHEGIERLSAFWTELGAPNRLADYDIDDSQVERMAEIA--TEQGELGNFKLLKK--QD 378
Query: 365 IKNLYLRAL 373
+ N+ AL
Sbjct: 379 VANILTAAL 387
>sp|Q04944|ADHA_CLOAB NADH-DEPENDENT BUTANOL DEHYDROGENASE A (BDH I)
>gi|144729|gb|AAA23206.1| (M96945) butanol dehydrogenase
[Clostridium acetobutylicum]
Length = 389
Score = 88.5 bits (216), Expect = 1e-16
Identities = 95/398 (23%), Positives = 176/398 (43%), Gaps = 43/398 (10%)
Query: 3 FFLKTTILMGRGSLENVKRIISE-GERVLI-FSSRSMDRLGFLSEVADYVEEAGGVYETI 60
+ + T + G+G ++ + I + G RVLI + S+ R G ++E + +
Sbjct: 6 YSIPTKVFFGKGKIDVIGEEIKKYGSRVLIVYGGGSIKRNGIYDRATAILKENNIAFYEL 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAK--AVKVFYDAPGLDFDSVAI 118
G+ P P + V++ + E + + +A+GGGS ID +K A V+YD G +D V
Sbjct: 66 SGVEPNPRITTVKKGIEICRENNVDLVLAIGGGSAIDCSKVIAAGVYYD--GDTWDMVKD 123
Query: 119 FSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILD 176
S+ K P+ + T S GSE+ +VI +T K + ++ P +++LD
Sbjct: 124 PSKITKVLPIASILT-------LSATGSEMDQIAVISNMETNEKLGVGHDDMRPKFSVLD 176
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPF-SDAL--AVKAARIIFERLEDSVNG 233
P +P+ D++ H E+Y S + D + A+ I + ++
Sbjct: 177 PTYTFTVPKNQTAAGTADIMSHTFESYFSGVEGAYVQDGIREAILRTCIKYGKIAMEKTD 236
Query: 234 DAKAREEVHYAATMAGIAFLNGRLGL-------VHAMSHK-SAWIG-PHGLVNAIFLPYV 284
D +AR + +A+++A +NG L L H M H+ SA+ HG+ AI P
Sbjct: 237 DYEARANLMWASSLA----INGLLSLGKDRKWSCHPMEHELSAYYDITHGVGLAILTPNW 292
Query: 285 MEF--NMERAREKYDAMARELGLSS-------ADELLEKVKELNEKLGVP-KLSEIVKEE 334
ME+ N + + G+ A E ++ +E LG+P KL E+ +
Sbjct: 293 MEYILNDDTLHKFVSYGINVWGIDKNKDNYEIAREAIKNTREYFNSLGIPSKLREVGIGK 352
Query: 335 EFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYLRA 372
+ L + + + + S P+ + ED+ ++ ++
Sbjct: 353 DKLELMAKQAVRNSGGTIGSLRPI--NAEDVLEIFKKS 388
>gb|AAD19418.1| (AF102543) NADH-dependent butanol dehydrogenase [Zymomonas mobilis]
Length = 385
Score = 88.5 bits (216), Expect = 1e-16
Identities = 92/348 (26%), Positives = 157/348 (44%), Gaps = 40/348 (11%)
Query: 3 FFLKTTILMGRGSLENVKRIISEGERVLI-FSSRSMDRLGFLSEVADYVEEAGGVYETII 61
++ T I+ G+G + + ++S+ RVL+ + S + G L EV + + Y
Sbjct: 6 YYNPTHIVFGKGRIAQLDTLLSKDARVLVLYGGSSAQKTGTLDEVRKALGDR--TYFEFG 63
Query: 62 GLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAI-FS 120
G+ P PS E + + + +V++ + +A+GGGSVID K V G ++ +
Sbjct: 64 GIEPNPSYETLMKAVEQVKQEKVDFLLAVGGGSVIDGTKFVAAAVPYEGEPWEILETDGK 123
Query: 121 RFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVI--KKGDTKYTIVSPELCPDYAILDPR 178
+ K+A P+ + T +P+T GSE++ SV+ K +K + + P ++ILDP
Sbjct: 124 KIKEALPVGTVLT----LPAT---GSEMNRNSVVTRKSIKSKRGFHNDHVFPVFSILDPT 176
Query: 179 LPEKMPREVARNSGLDVLVHAIEAYVS-----KASTPFSDALAVKAARIIFERLEDSVNG 233
+P N +D +H E Y++ F++ L +I E L+D N
Sbjct: 177 KVYTLPPRQLANGVVDSFIHITEQYLTYPVDGMVQDEFAEGLLRTLIKIGPELLKDQKNY 236
Query: 234 DAKAREEVHYAATMAGIAFLNGRLG-------LVHAMSHK--SAWIGPHGLVNAIFLPYV 284
D A + AT+A LNG +G H + H+ +A+ HG AI LP +
Sbjct: 237 DLAA--NFMWTATLA----LNGLIGAGVPQDWATHMVGHELTAAFGIDHGRTLAIILPSL 290
Query: 285 MEFNMERAREK---YDAMARELGLSSADE----LLEKVKELNEKLGVP 325
++ E + K Y + S DE +EK + E LG+P
Sbjct: 291 LQNQREAKKGKLLQYAKNVWHIDQGSDDERIDAAIEKTRHFFESLGIP 338
>gb|AAG23601.1|AF244652_1 (AF244652) iron alcohol dehydrogenase [Carboxydothermus
hydrogenoformans]
Length = 182
Score = 88.5 bits (216), Expect = 1e-16
Identities = 68/186 (36%), Positives = 104/186 (55%), Gaps = 18/186 (9%)
Query: 92 GGSVIDVAKAVKVFYDAPGL--DFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVS 149
G S D AK + + G D++ V K A+P+ L+A+ +T+G SE++
Sbjct: 1 GSSPHDCAKGIGLVATNGGSIKDYEGVN-----KSAKPM----LPLVAVNTTAGTASEMT 51
Query: 150 AASVIKKGD--TKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKA 207
S+I D K IV + P A+ DP L +MP+ + +G+D L HAIEAYVS
Sbjct: 52 RFSIITDEDRHVKMAIVDWHVTPIMAVNDPELMVEMPKALTAATGMDALTHAIEAYVSID 111
Query: 208 STPFSDALAVKAARIIFERLEDSV-NG-DAKAREEVHYAATMAGIAFLNGRLGLVHAMSH 265
+TP +DA A+KA +IF+ L+ +V NG D +AR+++ YA + G+ L +L VHAM
Sbjct: 112 ATPVTDAAALKAIDLIFKYLKRAVENGKDIEARDKMAYAEYLPGLP-LTMQLRYVHAM-- 168
Query: 266 KSAWIG 271
++W G
Sbjct: 169 PTSWEG 174
>pir||A47013 butanol dehydrogenase (EC 1.1.1.-) I - Clostridium acetobutylicum
Length = 389
Score = 88.2 bits (215), Expect = 1e-16
Identities = 95/395 (24%), Positives = 174/395 (44%), Gaps = 43/395 (10%)
Query: 3 FFLKTTILMGRGSLENVKRIISE-GERVLI-FSSRSMDRLGFLSEVADYVEEAGGVYETI 60
+ + T + G+G ++ + I + G RVLI + S+ R G ++E + +
Sbjct: 6 YSIPTKVFFGKGKIDVIGEEIKKYGSRVLIVYGGGSIKRNGIYDRATAILKENNIAFYEL 65
Query: 61 IGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAK--AVKVFYDAPGLDFDSVAI 118
G+ P P + V++ + E + + +A+GGGS ID +K A V+YD G +D V
Sbjct: 66 SGVEPNPRITTVKKGIEICRENNVDLVLAIGGGSAIDCSKVIAAGVYYD--GDTWDMVKD 123
Query: 119 FSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILD 176
S+ K P+ + T S GSE+ +VI +T K + ++ P +++LD
Sbjct: 124 PSKITKVLPIASILT-------LSATGSEMDQIAVISNMETNEKLGVGHDDMRPKFSVLD 176
Query: 177 PRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPF-SDAL--AVKAARIIFERLEDSVNG 233
P +P+ D++ H E+Y S + D + A+ I + ++
Sbjct: 177 PTYTFTVPKNQTAAGTADIMSHTFESYFSGVEGAYVQDGIREAILRTCIKYGKIAMEKTD 236
Query: 234 DAKAREEVHYAATMAGIAFLNGRLGL-------VHAMSHK-SAWIG-PHGLVNAIFLPYV 284
D +AR + +A+++A +NG L L H M H+ SA+ HG+ AI P
Sbjct: 237 DYEARANLMWASSLA----INGLLSLGKDRKWSCHPMEHELSAYYDITHGVGLAILTPNW 292
Query: 285 MEF--NMERAREKYDAMARELGLSS-------ADELLEKVKELNEKLGVP-KLSEIVKEE 334
ME+ N + + G+ A E ++ +E LG+P KL E+ +
Sbjct: 293 MEYILNDDTLHKFVSYGINVWGIDKNKDNYEIAREAIKNTREYFNSLGIPSKLREVGIGK 352
Query: 335 EFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLY 369
+ L + + + + S P+ + ED+ ++
Sbjct: 353 DKLELMAKQAVRNSGGTIGSLRPI--NAEDVLEIF 385
>sp|Q45072|TFTE_BURCE MALEYLACETATE REDUCTASE >gi|2120655|pir||I40177 maleylacetate
reductase (EC 1.3.1.32) [validated] - Pseudomonas
cepacia >gi|643636|gb|AAC43333.1| (U19883) maleylacetate
reductase [Burkholderia cepacia]
Length = 352
Score = 85.0 bits (207), Expect = 1e-15
Identities = 87/355 (24%), Positives = 148/355 (41%), Gaps = 49/355 (13%)
Query: 9 ILMGRGSLENVKRIISE--GERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPE 66
++ G G+L+++ R I R L+ S+ AD AG + +G+ P
Sbjct: 13 VVFGAGALQHLVREIDAMGSTRALVLSTPEQS--------ADAERVAGILGSRAVGVFPR 64
Query: 67 PS----VENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRF 122
+ +E E + + +A+GGGS + KA+ + P
Sbjct: 65 ATMHVPIELAREARLEASRLGADCAVAIGGGSTTGLGKAIALESGLP------------- 111
Query: 123 KKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEK 182
+++IP+T AGSE++ + K T + P I DP L
Sbjct: 112 ------------ILSIPTTY-AGSEMTPIYGVTDNGVKQTGRDARVLPRTVIYDPELTLG 158
Query: 183 MPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLE--DSVNGDAKAREE 240
+P + +SGL+ + HA E+ + P +A + R I L + D AR +
Sbjct: 159 LPIRMTVSSGLNAIAHAAESLYAHDGNPVIGLMAEEGIRAIGAALTPLQANPADLVARSD 218
Query: 241 VHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDA 298
Y A + G +GL H + H A+ PH ++ + LP+ + +N RA + +
Sbjct: 219 ALYGAWLCGAVLGAVSMGLHHKLCHTLGGAFNLPHAELHTVILPHALAYNSARASQAMER 278
Query: 299 MARELGLSSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKT-YEDP 351
+AR LG+SSA L +L E+ G+P L+ + E + DM+ K Y +P
Sbjct: 279 IARALGVSSAPRGL---FDLAERSGLPVSLAALGMPREGIELAADMAVKNPYPNP 330
>gb|AAB07597.1| (U68173) alcohol dehydrogenase [Salmonella typhimurium]
Length = 878
Score = 84.7 bits (206), Expect = 2e-15
Identities = 108/409 (26%), Positives = 166/409 (40%), Gaps = 57/409 (13%)
Query: 5 LKTTILMGRGSLE-NVKRIISEG-ERVLIFSSR-----------SMDRLGFLSEVADYVE 51
L +I RGSL + +I++G +R LI + R +R G +EV VE
Sbjct: 451 LPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFCSTTVSDRSLCAERRGVETEVFFEVE 510
Query: 52 EAGGVYETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGL 111
A +P++ V + F P+ IA+GG + + + P
Sbjct: 511 AA------------DPTLSVVRKGPELANSFKPDVIIAVGGVPRWTRGEIIGSCTNHPET 558
Query: 112 DFDSVAI-FSRFKKAQPLPKL-KTRLIAIPSTSGAGSEVSAASVIKKG--DTKYTIVSPE 167
+ F + PK+ K + +TSG GS++ + KY +
Sbjct: 559 RLIEERVRFMTSYRIYKFPKMVKAKCSPSTTTSGTGSKLHRLRLCPTTLLPQKYPLADYA 618
Query: 168 LCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERL 227
+ PD AI+D L MP + R L L HA+E VS ++ SD A++A + F
Sbjct: 619 VTPDMAIVDANLVMDMPNTLTRKGPLHRLTHAMERIVSVLASQ-SDGQALQALKEYFPAS 677
Query: 228 EDSVNGDAKAREEVHYAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIF----- 280
ARE +H AAT+A IAF N LG+ H M+HK + PHG NA +
Sbjct: 678 YHEGKNPV-ARERLHSAATIAPIAFANAFLGVCHWMAHKLPAQLHIPHGPFNARYRHSVR 736
Query: 281 ------------LPYVMEF-----NMERAREKYDAMARELGLSSADELLEKVKELNEKLG 323
Y+ F ER+ + R L VK + LG
Sbjct: 737 RAQSNPTKQVALSQYLYNFAAHRWPAERSIPRARTGIRPRKYKLVPVALCHVKGIKADLG 796
Query: 324 VPK-LSEI-VKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVEDIKNLYL 370
+PK + E V+E +FL V+ +S ++D NP P + ++K + L
Sbjct: 797 IPKSIREAGVQEADFLAHVDKLSEDAFDDQCTGANPRYPLMSELKQILL 845
>sp|P94135|TFF2_ALCEU MALEYLACETATE REDUCTASE II >gi|1747424|gb|AAC44727.1| (U16782)
maleylacetate reductase [Ralstonia eutropha]
Length = 359
Score = 84.7 bits (206), Expect = 2e-15
Identities = 76/307 (24%), Positives = 128/307 (40%), Gaps = 36/307 (11%)
Query: 4 FLKTTILMGRGSLENVKRIISE--GERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETII 61
F ++ G GS E + + R L+ + L + D + G ++ +
Sbjct: 12 FWPVRVVFGAGSTERIPAEVKRLGARRALVLCTPDQRDLA-QRVLGDLGDLGAGFHDGAV 70
Query: 62 GLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSR 121
PE SV + + + +A+GGGS I +AKA+ + +
Sbjct: 71 MHVPEASVTRAAQA---ARDADADLLVAVGGGSTIGLAKALALHHGM------------- 114
Query: 122 FKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPE 181
R +A+P+T AGSE++ + K T P + P + DP
Sbjct: 115 ------------RFVALPTTY-AGSEMTPIWGLTADGAKRTGRDPRVLPSTVLYDPHHLT 161
Query: 182 KMPREVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVN--GDAKARE 239
+P EV SG++ + HA+E+ + P + LA ++ R + + L +V+ GD AR
Sbjct: 162 SLPPEVTGPSGMNAIAHAVESMYAPDRNPITMLLAEESIRAMAQGLPVAVDSPGDLDART 221
Query: 240 EVHYAATMAGIAFLNGRLGLVHAMSH--KSAWIGPHGLVNAIFLPYVMEFNMERAREKYD 297
YAA +AG +GL H + H + PH ++A+ LP+V FN A +
Sbjct: 222 RTLYAAWLAGTVLGMVSMGLHHKLCHVLGGRFNLPHAPMHAVLLPHVAAFNEVAAPAELG 281
Query: 298 AMARELG 304
+A LG
Sbjct: 282 RVAAALG 288
>sp|Q24857|ADH3_ENTHI ALCOHOL DEHYDROGENASE 3 (ADH) >gi|1381024|dbj|BAA08651.1| (D49910)
putative NADH dependent alcohol dehydrogenase [Entamoeba
histolytica]
Length = 395
Score = 84.3 bits (205), Expect = 2e-15
Identities = 100/384 (26%), Positives = 177/384 (46%), Gaps = 51/384 (13%)
Query: 3 FFLKTTILMGRGSLENVKR--IISEGERVLI-FSSRSMDRLGFLSEVADYVE---EAGGV 56
++ ++ G+GSL+ +++ +I E R+++ + S+ + G EV +++ E GG+
Sbjct: 8 YYNPVRLIYGKGSLDEIEKQHLIPEDARIMMTYGGGSIKKNGVYEEVLKHIKPIVEFGGI 67
Query: 57 YETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLD--FD 114
P PS E + + +E +A+GGGS+ID K + + + D +D
Sbjct: 68 -------EPNPSHETCIKAIKIAKENKINFLVAVGGGSIIDATKYIALGMEHTYSDDPYD 120
Query: 115 SVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIV--SPELCPDY 172
+FK K+ ++ IP+T GSE + VI + K + + + P +
Sbjct: 121 ICLKGGKFKVNPAQAKIGV-VLTIPAT---GSETNCWGVISRHADKLKLPFNNESVFPTW 176
Query: 173 AILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKAS-TPFSDALAVKAARIIF----ERL 227
+I+DP +P RN +D VH IE Y+ P +A R I + L
Sbjct: 177 SIVDPCFTMSLPDNQIRNGLVDSFVHCIEQYIGNYHLNPVVEAETEGVMRTIIGVSHKTL 236
Query: 228 EDSVNGDAKAREEVHYAATMA-------GIAFLNGR-------LGLVHAMSHK-SAWIG- 271
E+ + D +AR YAAT+A G+ G G HA+ H+ +++ G
Sbjct: 237 EN--HQDYQARITFCYAATVALNMSLLCGVTLCGGAHAVGVTLCGGAHAVGHELTSYYGL 294
Query: 272 PHGLVNAIFLPYVMEFNMERAREKYDAMAREL-GL--SSADELLEKVKELNEKLGV-PKL 327
HG AI P VM FN E+ +K M ++ G+ S+ + +E ++ + +G+ +L
Sbjct: 295 AHGETLAITTPGVMRFNKEKNAKKLIQMGEQVFGIKNSTPEAAIEATEKWFKSIGMKTRL 354
Query: 328 SEIVKEEEFLGKVEDMSRKTYEDP 351
SE K +E + E ++RK +P
Sbjct: 355 SEWGKGKE---EFETIARKFEGNP 375
>sp|Q46856|YQHD_ECOLI HYPOTHETICAL OXIDOREDUCTASE IN METC-SUFI INTERGENIC REGION
>gi|7431288|pir||A65088 hypothetical protein b3011 -
Escherichia coli (strain K-12) >gi|882540|gb|AAA69178.1|
(U28377) ORF_o387 [Escherichia coli]
>gi|1789386|gb|AAC76047.1| (AE000383) putative
oxidoreductase [Escherichia coli K12]
Length = 387
Score = 82.7 bits (201), Expect = 6e-15
Identities = 98/347 (28%), Positives = 149/347 (42%), Gaps = 44/347 (12%)
Query: 7 TTILMGRGSLENVKRIISEGERVLI-FSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPP 65
T IL G+G++ ++ I RVLI + S+ + G L +V D ++ G G+ P
Sbjct: 10 TRILFGKGAIAGLREQIPHDARVLITYGGGSVKKTGVLDQVLDALK--GMDVLEFGGIEP 67
Query: 66 EPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFS----R 121
P+ E + + V E +A+GGGSV+D K + + P + D I
Sbjct: 68 NPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTKFIAAAANYPE-NIDPWHILQTGGKE 126
Query: 122 FKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDT--KYTIVSPELCPDYAILDPRL 179
K A P+ + T +P+T GSE +A +VI + T K S + P +A+LDP
Sbjct: 127 IKSAIPMGCVLT----LPAT---GSESNAGAVISRKTTGDKQAFHSAHVQPVFAVLDPVY 179
Query: 180 PEKMPREVARNSGLDVLVHAIEAYVSK-ASTPFSDALAVKAARIIFE----RLEDSVNGD 234
+P N +D VH +E YV+K D A + E L++ N D
Sbjct: 180 TYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAEGILLTLIEDGPKALKEPENYD 239
Query: 235 AKAREEVHYAATMAGIAFLNGRLG-------LVHAMSHK-SAWIG-PHGLVNAIFLPYVM 285
+A V +AAT A LNG +G H + H+ +A G H AI LP +
Sbjct: 240 VRA--NVMWAATQA----LNGLIGAGVPQDWATHMLGHELTAMHGLDHAQTLAIVLPALW 293
Query: 286 EFNMERAREK---YDAMARELGLSSADELLE----KVKELNEKLGVP 325
+ R K Y + S DE ++ + E+LGVP
Sbjct: 294 NEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFFEQLGVP 340
>sp|P27137|TFF1_ALCEU MALEYLACETATE REDUCTASE I >gi|95490|pir||D35255 probable
trans-chlorodienelactone isomerase - Alcaligenes
eutrophus >gi|150770|gb|AAA98265.1| (M35097) tfdF gene
product [Plasmid pJP4]
Length = 354
Score = 81.1 bits (197), Expect = 2e-14
Identities = 75/288 (26%), Positives = 121/288 (41%), Gaps = 39/288 (13%)
Query: 74 EVLPKVE----EFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLP 129
+V+ K E + ++ IA+GGGS +AK + + D P L
Sbjct: 72 DVIQKAERAFNDTDADSIIAIGGGSTTGLAKILSMNLDVPSL------------------ 113
Query: 130 KLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMPREVAR 189
IP+T AGSE++ + +G K T P++ P I DP L +P ++
Sbjct: 114 -------VIPTTY-AGSEMTTIWGVTEGGMKRTGRDPKVLPKTVIYDPLLTVDLPLAISV 165
Query: 190 NSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEVHYAATM 247
S L+ + HA E S P + + + +F+ + V DA+AR + + A M
Sbjct: 166 TSALNAIAHAAEGLYSADLNPVLETMCKQGICALFDAIPRLVAKPTDAEARTDALFGAWM 225
Query: 248 AGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELGL 305
G A + +GL H + H PH +AI LP+ + +N+ A + G
Sbjct: 226 CGTALCHLGMGLHHKLCHTLGGTLNLPHAETHAIVLPHALAYNLPYAAPAERLLQEVAGS 285
Query: 306 SSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMS-RKTYEDP 351
S L +L G P L+EI E + +V D++ R Y +P
Sbjct: 286 SDVPSAL---YDLARNAGAPLSLAEIGMRPEDIPRVRDLALRDQYPNP 330
>sp|O84992|MCA1_RHOOP MALEYLACETATE REDUCTASE I >gi|3387795|gb|AAC38802.1| (AF030176)
maleylacetate reductase [Rhodococcus opacus]
Length = 357
Score = 78.0 bits (189), Expect = 2e-13
Identities = 75/308 (24%), Positives = 132/308 (42%), Gaps = 40/308 (12%)
Query: 9 ILMGRGSLENVKRIISE--GERVLIFSSRSMDRLGFLSEVADYVEEAGG--VYETIIGLP 64
++ G G + NV+ + RVL+ S + L EV D V++ G G
Sbjct: 17 VIFGAGRMRNVREEVERLGRGRVLLLGSEN------LREVCDQVQDLLGELFVNRYDGAA 70
Query: 65 PEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKK 124
VE + L ++ + +A+GGGS +AKA+ G+D
Sbjct: 71 MHTPVEVTDIALAQLRTSEADCVVAIGGGSTTGLAKALAA---RTGVD------------ 115
Query: 125 AQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMP 184
+ +P+T AGSEV+ K T + + P+ I D L + +P
Sbjct: 116 ----------QVILPTTY-AGSEVTPVLGETVEGRKTTRSTLAVLPETVIYDVELSKNLP 164
Query: 185 REVARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDA--KAREEVH 242
+A S ++ L HA+EA S + P D A++AA+ + L V+ + + R ++
Sbjct: 165 VPIAVASAVNALAHAVEAMYSPDANPVVDTWALEAAQALARGLRGLVSDPSCRRIRTDLL 224
Query: 243 YAATMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMA 300
+ +AG+ + + + H + H A+ PH + + LPY M FN + D++A
Sbjct: 225 RGSWLAGMCLGSVGMAVHHKLCHTLGGAFGLPHAPTHTVVLPYAMSFNASEVPDVMDSLA 284
Query: 301 RELGLSSA 308
+ +S+A
Sbjct: 285 SAMNVSNA 292
>gb|AAK42626.1| Maleylacetate reductase (clcE) [Sulfolobus solfataricus]
Length = 327
Score = 75.7 bits (183), Expect = 8e-13
Identities = 85/363 (23%), Positives = 155/363 (42%), Gaps = 54/363 (14%)
Query: 7 TTILMGRGSLENVKRIISEGERVLIFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPE 66
T ++ G +L+ + + +G+R+ + ++RS+ + L ++ + E I G
Sbjct: 9 TQVIYGLNALDWLASV--KGKRIALVTTRSLLKSKILEQILSLINA-----EVIEGPRQH 61
Query: 67 PSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQ 126
V +V + +++ + + I LGGGSVID K L F+
Sbjct: 62 TPVGDVNNLTERLKGY--DVVIGLGGGSVIDGIK----------LSFNGY---------- 99
Query: 127 PLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMPRE 186
IAIP+T + G K I D ILDPR + P+
Sbjct: 100 --------YIAIPTTFSGAEHTRSGGATIDGIKKSRIGKE---ADAIILDPRATLETPKW 148
Query: 187 VARNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFERLEDSVNGDAKAREEVHYAAT 246
+ SG+ + HA+EA SK STPF+D+LA++ + + + + D + + +A ++
Sbjct: 149 LLIASGVRAIDHAVEALYSKDSTPFTDSLAIEGYKKLVKCIRDLDSIENRALCQI--GTW 206
Query: 247 MAGIAFLNGRLGLVHAMS--HKSAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELG 304
++ + ++G+ H + + PHG+ + I LP ++ N AR K + E
Sbjct: 207 LSSLTMRYAKMGISHNFGYVYGPRFNIPHGVTSCISLPSTIKLNYSVARNKLREIENE-- 264
Query: 305 LSSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKVEDMSRKTYEDPLVSFNPVEPSVE 363
+ L E + + + +G +LSE +E L V+ + S NPV+ +E
Sbjct: 265 ---GEPLYEFMDKFLKDIGAKRRLSEFTTLDEALKYVQTFIQIVNN----SGNPVKIDIE 317
Query: 364 DIK 366
K
Sbjct: 318 TAK 320
>gb|AAD55886.1|AF130250_3 (AF130250) maleylacetate reductase [Ralstonia eutropha]
Length = 360
Score = 75.7 bits (183), Expect = 8e-13
Identities = 72/275 (26%), Positives = 116/275 (42%), Gaps = 38/275 (13%)
Query: 88 IALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSE 147
+A+GGGS I +AKA+ + + P +IA+P+T AGSE
Sbjct: 90 VAIGGGSTIGLAKAIALESELP-------------------------VIAVPTTY-AGSE 123
Query: 148 VSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKA 207
++ + K T P + P + DP L +P ++ SG++ + HA E +
Sbjct: 124 MTPIYGLTDAGLKRTGRDPRVLPRTILYDPDLSLGLPVPISVTSGMNAIAHAAEGLYASD 183
Query: 208 STPFSD-------ALAVKAARIIFERLEDSVNGDAKAREEVHYAATMAGIAFLNGRLGLV 260
P S A +A +I + + +AR + Y A + G + GL
Sbjct: 184 RNPVSQWMSREGIAAFGRALPVIAAAEQHTDEARREARSDALYGAWLCGAVLGSVTAGLH 243
Query: 261 HAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMARELGLSSADELLEKVKEL 318
H + H + PH V+ + LP+ + +N A E A+A+ LG L V EL
Sbjct: 244 HKLCHTLGGTFNLPHAEVHTVVLPHALAYNAPAAPEAMAAIAQALGAGHGSGPL-AVFEL 302
Query: 319 NEKLGVP-KLSEIVKEEEFLGKVEDMS-RKTYEDP 351
KLG P L E+ E L + +++ R Y +P
Sbjct: 303 AAKLGAPTSLRELGLSSEDLDRACELALRDQYPNP 337
>pir||S68143 alcohol dehydrogenase (acceptor) (EC 1.1.99.8) - Entamoeba
histolytica >gi|1502307|emb|CAA88639.1| (Z48752) alcohol
dehydrogenase 3 [Entamoeba histolytica]
Length = 383
Score = 74.1 bits (179), Expect = 2e-12
Identities = 95/371 (25%), Positives = 171/371 (45%), Gaps = 37/371 (9%)
Query: 3 FFLKTTILMGRGSLENVKR--IISEGERVLI-FSSRSMDRLGFLSEVADYVE---EAGGV 56
++ ++ G+GSL+ +++ +I E R+++ + S+ + G EV +++ E GG+
Sbjct: 8 YYNPVRLIYGKGSLDEIEKQHLIPEDARIMMTYGGGSIKKNGVYEEVLKHIKPIVEFGGI 67
Query: 57 YETIIGLPPEPSVENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLD--FD 114
P PS E + + +E +A+GGGS+ID K + + + D +D
Sbjct: 68 -------EPNPSHETCIKAIKIAKENKINFLVAVGGGSIIDATKYIALGMEHTYSDDPYD 120
Query: 115 SVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIV--SPELCPDY 172
+FK K+ ++ IP+T GSE + VI + K + + + P +
Sbjct: 121 ICLKGGKFKVNPAQAKIGV-VLTIPAT---GSETNCWGVISRHADKLKLPFNNESVFPTW 176
Query: 173 AILDPRLPEKMPREVARNSGLDVLVHAIEAYVSKAS-TPFSDALAVKAARIIF--ERLED 229
+I+DP +P N +D VH IE Y+ P +A R I
Sbjct: 177 SIVDPCFTMSLPDNQIINGLVDSFVHCIEQYIGNYHLNPVVEAETEGVMRTIIGVSHKHK 236
Query: 230 SVNGDAKAREEVHYAATMA-GIAFLNG--RLGLVHAMSHK-SAWIG-PHGLVNAIFLPYV 284
+ +AR YAAT+A ++ L G G HA+ H+ +++ G HG AI P
Sbjct: 237 KIIKTIQARITFCYAATVALNMSLLCGVTLCGGAHAVGHELTSYYGLAHGETLAITTP-G 295
Query: 285 MEFNMERAREKYDAMAREL-GL--SSADELLEKVKELNEKLGV-PKLSEIVKEEEFLGKV 340
EFN E+ +K M ++ G+ S+ + +E ++ + +G+ +LSE K +E +
Sbjct: 296 NEFNKEKNAKKLIQMGEQVFGIKNSTPEAAIEATEKWFKSIGMKTRLSEWGKGKE---EF 352
Query: 341 EDMSRKTYEDP 351
E ++RK +P
Sbjct: 353 ETIARKFEGNP 363
>gb|AAF45302.1|AF239724_1 (AF239724) alcohol dehydrogenase [Giardia intestinalis]
Length = 408
Score = 73.0 bits (176), Expect = 5e-12
Identities = 86/367 (23%), Positives = 158/367 (42%), Gaps = 32/367 (8%)
Query: 31 IFSSRSMDRLGFLSEVADYVEEAGGVYETIIGLPPEPSVENVEEVLPKVE-EFSPETFIA 89
+ S + + G + + E G Y G+ P+VE ++E + ++ P +
Sbjct: 49 VSSPTAYQKCGLWDTITKALAERGIEYLHYNGISTNPTVEQIDEAVDLFRAKYGPGFLVC 108
Query: 90 -LGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPLPKLKTRLIAIPSTSGAGSEV 148
+GGGS D AKAV + L++ + +K + PK +++L+ + T G G+E
Sbjct: 109 GIGGGSPCDSAKAVSIL-----LEYSNNKARELYK-GEFEPKCRSKLVLVNLTHGTGTEC 162
Query: 149 SAASV--IKKGDT---KYTIVSPELCPDYAILDPRLPEKMPREVARNSGLDVLVHAIEAY 203
SV I G+ K I L PDY+I D + ++ R + +D L H +EA
Sbjct: 163 DRYSVASILTGELYPIKSGIGFEFLYPDYSIDDINGLFSLSPDMVRFTTIDALNHVMEAS 222
Query: 204 VSKASTPFSDALAVKAARIIFERLEDSV--NGDAKAREEVHYAATMAGIAFLNGRLGLVH 261
+ TP+ L +A +I L ++ + +AR + YAA +AG+A L H
Sbjct: 223 TTTIMTPYVCTLTREAVYLIVRYLPIALAEPDNLRARYWLSYAAAIAGMAIDESGCHLTH 282
Query: 262 AMSHKSAWIGP---HGLVNAIFLPYVMEFNMERAREKYDAMAREL------GLSSADELL 312
M H + P HG A+ P V++ + + + R + A++
Sbjct: 283 LMEHTLSAYRPRLAHGRGLALLQPAVLKHIWPKVCGYLNDLFRPILGNLKGTPDEAEQAF 342
Query: 313 EKVKELNEKLGVPKLSEIVKEEEF-LGKVEDMSRKTY----EDPLVSFNPVEPSVEDIKN 367
+++ + K+G S + +E F L V+ ++ T +S PV S D++
Sbjct: 343 RRMRAFHNKVG---FSGTLTDEGFNLEDVDTLTDATLAYYGAKATLSITPVPTSRSDLQR 399
Query: 368 LYLRALH 374
++ + +
Sbjct: 400 IFAESFY 406
>gb|AAF59422.1| (U32188) putative chloromaleylacetate reductase [Achromobacter
xylosoxidans subsp. denitrificans]
Length = 357
Score = 71.8 bits (173), Expect = 1e-11
Identities = 68/290 (23%), Positives = 121/290 (41%), Gaps = 37/290 (12%)
Query: 69 VENVEEVLPKVEEFSPETFIALGGGSVIDVAKAVKVFYDAPGLDFDSVAIFSRFKKAQPL 128
VE + L +V + + IALGGGS I ++KA+ + D P L
Sbjct: 71 VEVTQAALERVRPWHADGIIALGGGSAIGLSKAIALHTDLPQL----------------- 113
Query: 129 PKLKTRLIAIPSTSGAGSEVSAASVIKKGDTKYTIVSPELCPDYAILDPRLPEKMPREVA 188
IP+T AGSE++ + K + ++ P+ I D L +P ++
Sbjct: 114 --------VIPTTY-AGSEMTPILGQTESGVKTAQRTLKVLPETVIYDVELTYSLPLAMS 164
Query: 189 RNSGLDVLVHAIEAYVSKASTPFSDALAVKAARIIFE---RLEDSVNGDAKAREEVHYAA 245
SG++ + H++EA + + P LA ++ +L D D R Y +
Sbjct: 165 ICSGINAMAHSVEALYASDTNPIIRQLAEAGIAALYSGLPKLRDDAR-DPAVRAATLYGS 223
Query: 246 TMAGIAFLNGRLGLVHAMSHK--SAWIGPHGLVNAIFLPYVMEFNMERAREKYDAMAREL 303
++ + +GL H + H A PH + + LP+ + FN R+ + + R +
Sbjct: 224 WLSASCLASTSMGLHHKLCHTLGGALNLPHAETHTVVLPHAIAFNAPNVRDAVEILKRAM 283
Query: 304 GLSSADELLEKVKELNEKLGVP-KLSEIVKEEEFLGKV-EDMSRKTYEDP 351
G + ++ ++ + GVP L +I E + KV + K Y +P
Sbjct: 284 G---GGGVAGRIYDIAKDAGVPVALRDIGMTESDIDKVTAAVMAKPYPNP 330
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.136 0.375
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 135192917
Number of Sequences: 2977
Number of extensions: 5642650
Number of successful extensions: 18178
Number of sequences better than 1.0e-10: 92
Number of HSP's better than 0.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 78
Number of HSP's that attempted gapping in prelim test: 17855
Number of HSP's gapped (non-prelim): 98
length of query: 375
length of database: 189,106,746
effective HSP length: 60
effective length of query: 315
effective length of database: 153,197,526
effective search space: 48257220690
effective search space used: 48257220690
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 166 (69.1 bits)