BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1530 (alkA) DE:3-methyladenine DNA glycosylase (alkA)
(276 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75036 3-methyladenine DNA glycosylase (alka) PAB1530 - Pyr... 558 e-158
pir||B71127 probable DNA-3-methyladenine glycosidase - Pyrococcu... 464 e-130
gi|11499700 3-methyladenine DNA glycosylase (alkA) [Archaeoglobu... 123 2e-27
pir||G69806 DNA-3-methyladenine glycosidase II homolog yfjP - Ba... 84 1e-15
emb|CAB40601.1| (AJ010139) DNA alkylation repair enzyme [Bacillu... 82 6e-15
sp|P37878|3MGA_BACSU DNA-3-METHYLADENINE GLYCOSYLASE (3-METHYLAD... 80 3e-14
pir||A48494 3-methyladenine DNA glycosylase - Bacillus subtilis 80 3e-14
dbj|BAB05468.1| (AP001513) DNA-3-methyladenine glycosidase [Baci... 74 2e-12
gb|AAD39589.1|AC007858_3 (AC007858) This gene is a member of PF|... 69 4e-11
>pir||E75036 3-methyladenine DNA glycosylase (alka) PAB1530 - Pyrococcus abyssi
(strain Orsay) >gi|5458699|emb|CAB50186.1| (AJ248287)
3-methyladenine DNA glycosylase (alkA) [Pyrococcus
abyssi]
Length = 276
Score = 558 bits (1422), Expect = e-158
Identities = 276/276 (100%), Positives = 276/276 (100%)
Query: 1 MMSMIDLAKTTHEMIKNGTWKYEKGIFWQALPQGIIGYDGENFIIPEGLSRKEGKIVKEK 60
MMSMIDLAKTTHEMIKNGTWKYEKGIFWQALPQGIIGYDGENFIIPEGLSRKEGKIVKEK
Sbjct: 1 MMSMIDLAKTTHEMIKNGTWKYEKGIFWQALPQGIIGYDGENFIIPEGLSRKEGKIVKEK 60
Query: 61 ISFILGLDTDLDSFYSEIEDSKFSFLIDEFYGLTIPAAPSLYQAMVEVIAQQQVSFEFAQ 120
ISFILGLDTDLDSFYSEIEDSKFSFLIDEFYGLTIPAAPSLYQAMVEVIAQQQVSFEFAQ
Sbjct: 61 ISFILGLDTDLDSFYSEIEDSKFSFLIDEFYGLTIPAAPSLYQAMVEVIAQQQVSFEFAQ 120
Query: 121 KAISNLVKLVGRKVNDLYLFPEPSDIIRLGDRIREAKLGYRASYILSITQEYLRGRLKLD 180
KAISNLVKLVGRKVNDLYLFPEPSDIIRLGDRIREAKLGYRASYILSITQEYLRGRLKLD
Sbjct: 121 KAISNLVKLVGRKVNDLYLFPEPSDIIRLGDRIREAKLGYRASYILSITQEYLRGRLKLD 180
Query: 181 LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGLRKNVYPAGDLGLRRGIAKIFGLNPKKVK 240
LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGLRKNVYPAGDLGLRRGIAKIFGLNPKKVK
Sbjct: 181 LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGLRKNVYPAGDLGLRRGIAKIFGLNPKKVK 240
Query: 241 EKDVREIIEPYGKWKSLLAFYILCYDRKTQKPGVRK 276
EKDVREIIEPYGKWKSLLAFYILCYDRKTQKPGVRK
Sbjct: 241 EKDVREIIEPYGKWKSLLAFYILCYDRKTQKPGVRK 276
>pir||B71127 probable DNA-3-methyladenine glycosidase - Pyrococcus horikoshii
>gi|3257193|dbj|BAA29876.1| (AP000003) 283aa long
hypothetical DNA-3-methyladenine glycosidase [Pyrococcus
horikoshii]
Length = 283
Score = 464 bits (1180), Expect = e-130
Identities = 219/270 (81%), Positives = 252/270 (93%)
Query: 1 MMSMIDLAKTTHEMIKNGTWKYEKGIFWQALPQGIIGYDGENFIIPEGLSRKEGKIVKEK 60
M+ MIDLAKTT+EMIKNGTWKYEKG FWQALPQGI+GYDGENFI+P+ L++++ K+VKEK
Sbjct: 1 MIGMIDLAKTTNEMIKNGTWKYEKGTFWQALPQGIVGYDGENFILPDELNKRDRKLVKEK 60
Query: 61 ISFILGLDTDLDSFYSEIEDSKFSFLIDEFYGLTIPAAPSLYQAMVEVIAQQQVSFEFAQ 120
I FILGLDTDLDSFYSEI DSKFSFLIDEFYGLTIPAAP++YQA+VEVIAQQQVSFEFAQ
Sbjct: 61 IKFILGLDTDLDSFYSEISDSKFSFLIDEFYGLTIPAAPNVYQAIVEVIAQQQVSFEFAQ 120
Query: 121 KAISNLVKLVGRKVNDLYLFPEPSDIIRLGDRIREAKLGYRASYILSITQEYLRGRLKLD 180
KAI NLV L G+K+NDLYLFP P DIIRLG++I+EAKLGYR +YILS+T+EYL+GRLKLD
Sbjct: 121 KAIKNLVILSGKKINDLYLFPGPQDIIRLGEKIKEAKLGYRGNYILSVTEEYLKGRLKLD 180
Query: 181 LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGLRKNVYPAGDLGLRRGIAKIFGLNPKKVK 240
L+D++E EA+ YLT+F+GIG+WSAELFLMYGLRKNVYPAGDLGLRRGIAKIF L+ KKV
Sbjct: 181 LRDMDEKEAIRYLTRFRGIGKWSAELFLMYGLRKNVYPAGDLGLRRGIAKIFNLSLKKVT 240
Query: 241 EKDVREIIEPYGKWKSLLAFYILCYDRKTQ 270
E+ VREIIEPYG+WKSLLAFYILCYDRKT+
Sbjct: 241 ERIVREIIEPYGRWKSLLAFYILCYDRKTE 270
>gi|11499700 3-methyladenine DNA glycosylase (alkA) [Archaeoglobus fulgidus]
>gi|7482882|pir||E69514 3-methyladenine DNA glycosylase
(alkA) homolog - Archaeoglobus fulgidus
>gi|2648411|gb|AAB89134.1| (AE000958) 3-methyladenine
DNA glycosylase (alkA) [Archaeoglobus fulgidus]
Length = 295
Score = 123 bits (307), Expect = 2e-27
Identities = 89/268 (33%), Positives = 143/268 (53%), Gaps = 18/268 (6%)
Query: 11 THEMIKNGTWKYEKGIFWQALPQGIIGY-DGENFIIPEG-LSRKEGKIVKEKISFILGLD 68
T +++++G W+ + I ++ Y + E I+ EG +E + V+ K+ LGL
Sbjct: 26 TPDVVESGVWR--RAIVLDGRAVAVMAYPESERTIVVEGNFENREWEAVRRKLVEYLGLQ 83
Query: 69 TDLDSFYSEIEDSKFSFLIDEFYGLTIPA--APSLYQAMVEVIAQQQVSFEFAQKAISNL 126
+ + D K L + FYG + S+++ + + I QQQ+SF A+K + +
Sbjct: 84 NPEELYRFMDGDEKLRMLKNRFYGFGRAGLMSMSVFEGIAKAIIQQQISFVVAEKLAAKI 143
Query: 127 VKLVGRKVN----DLYLFPEPSDIIRLG-DRIREAKLGYR-ASYILSITQEYLRGRLKLD 180
V G +V Y FP I++ G + +RE L R A I+ I +E L+ +
Sbjct: 144 VGRFGDEVEWNGLKFYGFPTQEAILKAGVEGLRECGLSRRKAELIVEIAKEE---NLE-E 199
Query: 181 LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGLRKNVYPAGDLGLRRGIAKIFGLNPKKVK 240
LK+ E+EA YLT FKGIGRW+AEL L L KNV+PA DLG+RR +++++ N +
Sbjct: 200 LKEWGEEEAYEYLTSFKGIGRWTAELVLSMALGKNVFPADDLGVRRAVSRLY-FNGEIQS 258
Query: 241 EKDVREII-EPYGKWKSLLAFYILCYDR 267
+ VREI E +G++ + FY+ YDR
Sbjct: 259 AEKVREIARERFGRFARDILFYLFLYDR 286
>pir||G69806 DNA-3-methyladenine glycosidase II homolog yfjP - Bacillus subtilis
>gi|2633125|emb|CAB12630.1| (Z99108) similar to
DNA-3-methyladenine glycosidase II [Bacillus subtilis]
>gi|2780400|dbj|BAA24301.1| (D78509) YfjP [Bacillus
subtilis]
Length = 287
Score = 84.3 bits (205), Expect = 1e-15
Identities = 61/228 (26%), Positives = 110/228 (47%), Gaps = 8/228 (3%)
Query: 36 IGYDGENFIIPEGLSRKEGKIVKEKISFILGLDTDLDSFYSEIEDSKFSFLIDEFYGLTI 95
+G+ GE + G +G+++KE I I + L + S + +E G +
Sbjct: 55 LGHAGEPEFLVSG-ETDQGEMMKE-IKRIFQWENHLQHVLDHFSKTSLSAIFEEHAGTPL 112
Query: 96 PAAPSLYQAMVEVIAQQQVSFEFAQKAISNLVKLVGRKVNDLYLFPEPSDIIRLG-DRIR 154
S+Y M++ I QQ++ FA V G + + ++ +P+P I L +R
Sbjct: 113 VLDYSVYNCMMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKPETIAELDYQDLR 172
Query: 155 EAKLGYR-ASYILSITQEYLRGRLKL-DLKDLNEDEAVNYLTKFKGIGRWSAELFLMYGL 212
+ + R A Y + ++ G L L +L + +++ + L K +GIG W+ + LM+GL
Sbjct: 173 DLQFSMRKAEYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGL 232
Query: 213 -RKNVYPAGDLGLRRGIAKIFGLNPKKVKE--KDVREIIEPYGKWKSL 257
R N++P D+GL+ I + F L+ K K+ + + EPY + SL
Sbjct: 233 GRPNLFPLADIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASL 280
>emb|CAB40601.1| (AJ010139) DNA alkylation repair enzyme [Bacillus cereus]
Length = 287
Score = 82.3 bits (200), Expect = 6e-15
Identities = 47/162 (29%), Positives = 88/162 (54%), Gaps = 6/162 (3%)
Query: 102 YQAMVEVIAQQQVSFEFAQKAISNLVKLVGRKVNDLYLFPEPSDIIRLG-DRIREAKLGY 160
+ ++ I QQ++ +FA VK G + N ++ FP P + + + +RE K
Sbjct: 119 FACLLRCIIHQQINLKFATVLTEQFVKRYGTEKNGVFFFPTPEIVANISIEELREQKFSQ 178
Query: 161 R-ASYILSITQEYLRGRLKLD-LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGL-RKNVY 217
R A Y++ + + + G+L L +++ E+ L +GIG W+ + FLM+GL RKN++
Sbjct: 179 RKAEYMVGLAKCIIGGKLDLTRIENETEEGVAAQLLPIRGIGAWTVQNFLMFGLGRKNMF 238
Query: 218 PAGDLGLRRGIAKIFGLN--PKKVKEKDVREIIEPYGKWKSL 257
P D+G++R + +F L+ P ++V++ EPY + +L
Sbjct: 239 PKADIGIQRAVQGVFQLDDKPDDAFLENVKQECEPYCSYAAL 280
>sp|P37878|3MGA_BACSU DNA-3-METHYLADENINE GLYCOSYLASE (3-METHYLADENINE-DNA GLYCOSIDASE)
>gi|7474403|pir||E69584 3-methyladenine DNA glycosylase
(EC 3.2.2.-) - Bacillus subtilis
>gi|436209|dbj|BAA03361.1| (D14465) 3-methyladenine DNA
glycosylase [Bacillus subtilis]
>gi|2632447|emb|CAB11956.1| (Z99104) DNA-3-methyladenine
glycosylase [Bacillus subtilis]
>gi|2632465|emb|CAB11973.1| (Z99105) DNA-3-methyladenine
glycosylase [Bacillus subtilis]
>gi|3599598|dbj|BAA33073.1| (AB006424) 3-METHYLADENINE
DNA GLYCOSYLASE [Bacillus subtilis]
Length = 303
Score = 80.0 bits (194), Expect = 3e-14
Identities = 61/205 (29%), Positives = 96/205 (46%), Gaps = 10/205 (4%)
Query: 67 LDTDLDSFYSEIE-DSKFSFLIDEFYGLTIPAAPSLYQAMVEVIAQQQVSFEFAQKAISN 125
LD DL FY + D +FYGL + P L++A+ + QQ++ FA
Sbjct: 95 LDNDLTPFYEMAKADPLLKMPARKFYGLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQ 154
Query: 126 LVKLVGRKV----NDLYLFPEPSDIIRLGDR-IREAKLGYRAS-YILSITQEYLRGRLKL 179
V+ G + ++FP I RL + + K+ + S YI+ I + G L
Sbjct: 155 FVEAFGDSIEWNGKKYWVFPPYERIARLTPTDLADIKMTVKKSEYIIGIARLMASGELSR 214
Query: 180 D-LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGLR-KNVYPAGDLGLRRGIAKIFGLNPK 237
+ L +N +A L K +GIG W+A LM LR +P D+GL I + +N K
Sbjct: 215 EKLMKMNFKDAEKNLIKIRGIGPWTANYVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRK 274
Query: 238 KVKEKDVREIIEPYGKWKSLLAFYI 262
K+ ++ EI P+ +W+S FY+
Sbjct: 275 PTKD-EILEISVPWKEWQSYATFYL 298
>pir||A48494 3-methyladenine DNA glycosylase - Bacillus subtilis
Length = 303
Score = 80.0 bits (194), Expect = 3e-14
Identities = 61/205 (29%), Positives = 96/205 (46%), Gaps = 10/205 (4%)
Query: 67 LDTDLDSFYSEIE-DSKFSFLIDEFYGLTIPAAPSLYQAMVEVIAQQQVSFEFAQKAISN 125
LD DL FY + D +FYGL + P L++A+ + QQ++ FA
Sbjct: 95 LDNDLTPFYEMAKADPLLKMPARKFYGLRVIGIPDLFEALCWGVLGQQINLAFAYSLKKQ 154
Query: 126 LVKLVGRKV----NDLYLFPEPSDIIRLGDR-IREAKLGYRAS-YILSITQEYLRGRLKL 179
V+ G + ++FP I RL + + K+ + S YI+ I + G L
Sbjct: 155 FVEAFGDSIEWNGKKYWVFPPYERIARLTPTDLADIKMTVKKSEYIIGIARLMASGELSR 214
Query: 180 D-LKDLNEDEAVNYLTKFKGIGRWSAELFLMYGLR-KNVYPAGDLGLRRGIAKIFGLNPK 237
+ L +N +A L K +GIG W+A LM LR +P D+GL I + +N K
Sbjct: 215 EKLMKMNFKDAEKNLIKIRGIGPWTANYVLMRCLRFPTAFPIDDVGLIHSIKILRNMNRK 274
Query: 238 KVKEKDVREIIEPYGKWKSLLAFYI 262
K+ ++ EI P+ +W+S FY+
Sbjct: 275 PTKD-EILEISVPWKEWQSYATFYL 298
>dbj|BAB05468.1| (AP001513) DNA-3-methyladenine glycosidase [Bacillus halodurans]
Length = 221
Score = 74.1 bits (179), Expect = 2e-12
Identities = 52/179 (29%), Positives = 93/179 (51%), Gaps = 15/179 (8%)
Query: 79 EDSKFSFLIDEFYGLTIPAAPSLYQAMVEVIAQQQVSFEFAQKAISNLVKLVGRKVNDLY 138
+DS+ I+ + +P P+ +Q++V I +QQ+S + A + +LVG +
Sbjct: 17 QDSRLFQFIEIAGEVQLPTKPNPFQSLVSSIVEQQLSIKAASAIYGRVEQLVGGALE--- 73
Query: 139 LFPEPSDIIRLGDR-IREAKLGYRA-SYILSITQEYLRGRLKL-DLKDLNEDEAVNYLTK 195
+P + R+ D +R+A + R YI + + GRL +L+ + LT
Sbjct: 74 ---KPEQLYRVSDEALRQAGVSKRKIEYIRHVCEHVESGRLDFTELEGAEATTVIEKLTA 130
Query: 196 FKGIGRWSAELFLMYGL-RKNVYPAGDLGLRRGIAKIFGLNPKKVKEKDVREIIEPYGK 253
KGIG+W+AE+F+M+ L R +V GD+GL+RG ++G E D ++++ +GK
Sbjct: 131 IKGIGQWTAEMFMMFSLGRLDVLSVGDVGLQRGAKWLYGNG-----EGDGKKLLIYHGK 184
>gb|AAD39589.1|AC007858_3 (AC007858) This gene is a member of PF|01332 Alkylbase DNA
glycosidases alkA family. [Oryza sativa]
Length = 334
Score = 69.5 bits (167), Expect = 4e-11
Identities = 48/164 (29%), Positives = 87/164 (52%), Gaps = 8/164 (4%)
Query: 102 YQAMVEVIAQQQVSFEFAQKAISNLVKLVGRKVNDLYLFPEPSDIIRLGDRIREAKLGYR 161
+ ++V I QQ++F+ A S + L+G + N L PE + D + +
Sbjct: 118 FHSLVRSILYQQLAFKAAASVYSRFLSLLGGEHNVL---PEAVLALTTQDLRQIGVSPRK 174
Query: 162 ASYILSITQEYLRGRLKLDLKDLNEDEA--VNYLTKFKGIGRWSAELFLMYGL-RKNVYP 218
ASY+ + ++Y G L D +N D+ LT KGIG WS +F+++ L R +V P
Sbjct: 175 ASYLHDLARKYASGILS-DAAVVNMDDRSLAAMLTMVKGIGAWSVHMFMIFSLNRPDVLP 233
Query: 219 AGDLGLRRGIAKIFGLNPKKVKEKDVREIIEPYGKWKSLLAFYI 262
A DLG+R+G+ ++GL+ + + ++ E + ++S+ A+Y+
Sbjct: 234 AADLGVRKGVQHLYGLDAVP-RPSQMEKLCEQWRPYRSVGAWYM 276
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.142 0.413
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104571011
Number of Sequences: 2977
Number of extensions: 4451341
Number of successful extensions: 9693
Number of sequences better than 1.0e-10: 9
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 9676
Number of HSP's gapped (non-prelim): 9
length of query: 276
length of database: 189,106,746
effective HSP length: 54
effective length of query: 222
effective length of database: 156,788,448
effective search space: 34807035456
effective search space used: 34807035456
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 164 (68.3 bits)