BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1559 (hypD) DE:hydrogenase expression/formation protein (hypD) (367 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75031 hydrogenase expression/formation protein (hypd) PAB1... 760 0.0 pir||G71076 probable hydrogenase expression/formation protein hy... 668 0.0 sp|Q58400|Y993_METJA PUTATIVE HYDROGENASE EXPRESSION/FORMATION P... 333 1e-90 gi|11498966 hydrogenase expression/formation protein (hypD) [Arc... 330 1e-89 pir||D69009 hydrogenase expression/formation protein HypD - Meth... 322 4e-87 pir||F70399 hydrogenase expression/formation protein HypD - Aqui... 289 3e-77 sp|P31903|HYPD_ALCEU HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 284 9e-76 sp|P42033|HUPD_AZOCH HYDROGENASE EXPRESSION/FORMATION PROTEIN HU... 279 3e-74 sp|P31882|HYPD_AZOVI HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 277 2e-73 pir||H81410 hydrogenase isoenzymes formation protein Cj0625 [imp... 273 3e-72 sp|P40598|HYPD_RHILV HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 272 5e-72 sp|P31904|HYPD_BRAJA HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 271 1e-71 pir||S74470 hydrogenase isoenzymes formation protein hypD - Syne... 262 4e-69 pir||B64632 hydrogenase expression/formation protein - Helicobac... 261 7e-69 pir||F71883 hydrogenase expression/formation protein - Helicobac... 259 5e-68 gb|AAA69239.1| (U29579) CG Site no. 33116; ORF_o373 [Escherichia... 243 2e-63 sp|P24192|HYPD_ECOLI HYDROGENASE ISOENZYMES FORMATION PROTEIN HY... 243 3e-63 pir||S59984 hypD protein - Rhodobacter capsulatus >gi|46055|emb|... 236 2e-61 sp|P26411|HYPD_RHOCA HYDROGENASE EXPRESSION/FORMATION PROTEIN HY... 236 2e-61 gb|AAB64435.1| (AF006594) HupD [Anabaena PCC7120] 146 4e-34 gb|AAF20001.1|AF214145_8 (AF214145) hydrogenase maturating prote... 69 6e-11 >pir||E75031 hydrogenase expression/formation protein (hypd) PAB1559 - Pyrococcus abyssi (strain Orsay) >gi|5458659|emb|CAB50146.1| (AJ248287) hydrogenase expression/formation protein (hypD) [Pyrococcus abyssi] Length = 367 Score = 760 bits (1940), Expect = 0.0 Identities = 367/367 (100%), Positives = 367/367 (100%) Query: 1 MLEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGP 60 MLEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGP Sbjct: 1 MLEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGP 60 Query: 61 GCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVV 120 GCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVV Sbjct: 61 GCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVV 120 Query: 121 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV 180 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV Sbjct: 121 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV 180 Query: 181 EALLKAGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMV 240 EALLKAGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMV Sbjct: 181 EALLKAGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMV 240 Query: 241 KEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEI 300 KEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEI Sbjct: 241 KEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEI 300 Query: 301 RTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIF 360 RTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIF Sbjct: 301 RTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIF 360 Query: 361 YKYGALF 367 YKYGALF Sbjct: 361 YKYGALF 367 >pir||G71076 probable hydrogenase expression/formation protein hypD - Pyrococcus horikoshii >gi|3257290|dbj|BAA29973.1| (AP000004) 367aa long hypothetical hydrogenase expression/formation protein hypD [Pyrococcus horikoshii] Length = 367 Score = 668 bits (1705), Expect = 0.0 Identities = 311/367 (84%), Positives = 344/367 (92%) Query: 1 MLEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGP 60 MLEKFR++ +AQ+I+ KI +E KG +E+RFMHVCGTHEDTVTR+GIRSLLP+NVKIMSGP Sbjct: 1 MLEKFRERAVAQRIIEKIKEEVKGMEEVRFMHVCGTHEDTVTRSGIRSLLPKNVKIMSGP 60 Query: 61 GCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVV 120 GCPVCITPVEDIV+MMEIM+ A+EEGE+IILTTFGDMY+IPTP GSFADLKS+G+DVR+V Sbjct: 61 GCPVCITPVEDIVKMMEIMKIAREEGEEIILTTFGDMYKIPTPRGSFADLKSQGYDVRIV 120 Query: 121 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV 180 YSIYDSYKIA+EN DKLVVHFSPGFETTTAPTAGMLE V+ EGL+NF+IYSVHRLTPPAV Sbjct: 121 YSIYDSYKIAKENPDKLVVHFSPGFETTTAPTAGMLEVVVEEGLENFRIYSVHRLTPPAV 180 Query: 181 EALLKAGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMV 240 EALLKAGTVF GLI PGHVSTIIGV+GW YLTEK+ IPQVVAGFEPVDVLL IL LIR+V Sbjct: 181 EALLKAGTVFQGLITPGHVSTIIGVKGWVYLTEKFGIPQVVAGFEPVDVLLAILTLIRLV 240 Query: 241 KEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEI 300 K GEAKI+NEY+R VKWEGNVKAQELI KFFEVKDAKWRALGIIP+SGLEL++EWK+LEI Sbjct: 241 KRGEAKIVNEYNRAVKWEGNVKAQELIRKFFEVKDAKWRALGIIPKSGLELRKEWKELEI 300 Query: 301 RTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIF 360 R Y+PDVPKLPDLEKGCLCGA+LRGLALP QCPHFGKTCTPRHPIGPCMVSYEGTC IF Sbjct: 301 RNYYDPDVPKLPDLEKGCLCGAVLRGLALPTQCPHFGKTCTPRHPIGPCMVSYEGTCHIF 360 Query: 361 YKYGALF 367 YKYGALF Sbjct: 361 YKYGALF 367 >sp|Q58400|Y993_METJA PUTATIVE HYDROGENASE EXPRESSION/FORMATION PROTEIN MJ0993 >gi|2127979|pir||A64424 hydrogenase expression/formation protein D - Methanococcus jannaschii >gi|1591656|gb|AAB98998.1| (U67542) hydrogenase expression/formation protein (hypD) [Methanococcus jannaschii] Length = 358 Score = 333 bits (846), Expect = 1e-90 Identities = 164/358 (45%), Positives = 232/358 (63%), Gaps = 9/358 (2%) Query: 7 DKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPGCPVCI 66 D+ + +K + KI+K A+ D+L+ MHVCG+HE T+ + GIR +LPEN+ ++ GPGCPVC+ Sbjct: 6 DRALIKKAIDKINKLAEKIDKLKIMHVCGSHEHTICKYGIRDVLPENITVVPGPGCPVCV 65 Query: 67 TPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVVYSIYDS 126 T ++I + + ++TT GDMYR+P S + +SEG DVR+VYSI ++ Sbjct: 66 TTQKEIDTAIYLADNG------YVITTLGDMYRVPGSEKSLMEKQSEGCDVRIVYSISEA 119 Query: 127 YKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAVEALLKA 186 K+A++ RDK V + GFETT T L ++ N+ ++NF I + HR TPP +E LL Sbjct: 120 VKMAKKERDKKFVFVAIGFETTAPTTGAELISLKNKDVNNFFILNCHRQTPPVMEFLLNE 179 Query: 187 GTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMVKEGEAK 246 G I PGHVSTI G++ + L EKY P VVAGFEP+DVL+ I+++++ V GEAK Sbjct: 180 GVYLDAFICPGHVSTITGLKPYYGLCEKYKAPMVVAGFEPIDVLMAIIMILKQVISGEAK 239 Query: 247 IINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEIRTLYNP 306 + NEY R VK EGNV AQ++IN+ FE D WR ++ G L+ ++K +I + Sbjct: 240 VENEYIRAVKPEGNVLAQKIINEVFESIDVPWRGFPVVKNGGFGLREKYKKFDI--YEHE 297 Query: 307 DVPKLPD-LEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIFYKY 363 D+P++ + + KGC+C ILRG LP CP FG CTP +P+G CMVS EGTC IFYKY Sbjct: 298 DIPEIKEKIPKGCICDKILRGEKLPTDCPLFGTVCTPLNPVGSCMVSDEGTCRIFYKY 355 >gi|11498966 hydrogenase expression/formation protein (hypD) [Archaeoglobus fulgidus] >gi|7447453|pir||A69421 hydrogenase expression/formation protein (hypD) homolog - Archaeoglobus fulgidus >gi|2649201|gb|AAB89873.1| (AE001009) hydrogenase expression/formation protein (hypD) [Archaeoglobus fulgidus] Length = 371 Score = 330 bits (838), Expect = 1e-89 Identities = 167/356 (46%), Positives = 236/356 (65%), Gaps = 15/356 (4%) Query: 13 KIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPGCPVCITPVEDI 72 ++ KI + +K F+ ++ MH+CGTHEDT+T+ +RSLLPEN+ ++SGPGCPVCITP EDI Sbjct: 22 RLSEKIRELSKEFESVKIMHLCGTHEDTITKYNLRSLLPENISLLSGPGCPVCITPDEDI 81 Query: 73 VRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVVYSIYDSYKIAQE 132 +M ++ K E+I L TFGDM R+P S L++EG+D+R+VYSI+DS K+A E Sbjct: 82 QMVMHLLEK-----ENITLATFGDMARVPFQGKSLFTLRAEGYDIRIVYSIFDSLKLA-E 135 Query: 133 NRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAVEALLKAGTVFHG 192 + D+ VVHF+ GFETT TA + + +NF ++S HR PAV AL + TV Sbjct: 136 SSDRPVVHFAIGFETTMPSTA----IALLDEKENFYVFSAHRFFIPAVHALCENTTV-DA 190 Query: 193 LIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMVKEGEAKIINEYS 252 I+PGHVSTIIGV+ + + +KY IPQV+AGFEP DV+L I +L+ + +G ++INEYS Sbjct: 191 FINPGHVSTIIGVKPYREILKKYGIPQVIAGFEPEDVMLAIYLLLEAMSDGRREVINEYS 250 Query: 253 RVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEIRTLYN---PDVP 309 R VK EGNV+A E +++ F +D WR LG++ SG L + ++ + R ++ D Sbjct: 251 RAVKEEGNVRALEAMDRVFRKEDWSWRGLGVVKGSGGALAKRFEQFDARKVFEDAFADFQ 310 Query: 310 KLPDLEKG-CLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIFYKYG 364 + D K C CG +L+G+A P CP F K C PR+P+G CMVS EGTC+I+ G Sbjct: 311 PVEDKSKRLCRCGDVLKGIATPKDCPLFMKACNPRNPVGACMVSVEGTCNIWATSG 366 >pir||D69009 hydrogenase expression/formation protein HypD - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622170|gb|AAB85561.1| (AE000877) hydrogenase expression/formation protein HypD [Methanobacterium thermoautotrophicum] Length = 348 Score = 322 bits (817), Expect = 4e-87 Identities = 153/359 (42%), Positives = 241/359 (66%), Gaps = 14/359 (3%) Query: 8 KEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPGCPVCIT 67 K +++++V +I + ++ ++ MHVCG+HE T+ + G+RSLLPE V++++GPGCPVC Sbjct: 2 KNLSRELVSRIQEISR---PVKIMHVCGSHEHTIMQHGVRSLLPEEVEVVAGPGCPVCCV 58 Query: 68 PVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVVYSIYDSY 127 P +I +E+ R+ + +TTFGDM R+P GS AD +++G DVR+VY + ++ Sbjct: 59 PAREIDECIELARQG------VTITTFGDMLRVPGSRGSLADARADGADVRIVYGVGNAV 112 Query: 128 KIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAVEALLKAG 187 +IA++ D+ VV + GFETT TA +++ +NF + S HRL PPA+ L+++G Sbjct: 113 EIARK-LDREVVFMAAGFETTAPTTAS---EILSGPPENFSVLSCHRLIPPALRFLIESG 168 Query: 188 TV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMVKEGEAK 246 V + LI+PGHVSTIIG++ + + YNIPQV+AGF P+D+L+ + +++R + GEAK Sbjct: 169 EVNLNALIEPGHVSTIIGMKPYEPFSRDYNIPQVIAGFNPLDILMAVYMILRQIDRGEAK 228 Query: 247 IINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEIRTLYNP 306 + NEY R VK EGN+KAQ+++++ F+V + +WR +IP+S E++ E+ + R ++ Sbjct: 229 VENEYKRAVKPEGNLKAQKVMDEVFKVTEREWRGFPVIPESVYEIRDEFSEFNAREKFDI 288 Query: 307 DVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIFYKYGA 365 +V D GC+CGA+LRG+A P +C F CTP PIG CMVS EGTC+I Y+Y + Sbjct: 289 EVEDTVDTPAGCICGAVLRGVARPEECKLFKNECTPTSPIGACMVSREGTCNIAYRYSS 347 >pir||F70399 hydrogenase expression/formation protein HypD - Aquifex aeolicus >gi|2983616|gb|AAC07185.1| (AE000726) hydrogenase expression/formation protein HypD [Aquifex aeolicus] Length = 380 Score = 289 bits (732), Expect = 3e-77 Identities = 141/373 (37%), Positives = 232/373 (61%), Gaps = 16/373 (4%) Query: 1 MLEKFRDKEIAQKIVRKIHKEAKGFDE-LRFMHVCGTHEDTVTRAGIRSLLPENVKIMSG 59 + E ++DKE K+ KI K ++ + M CG H + + GI LL V+ + G Sbjct: 8 IFEAYKDKERVLKLSEKIKKLSEKIGRTVNIMEFCGGHTHAIMKYGIDQLLKGVVRFIHG 67 Query: 60 PGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIP-TPMGSFADLKSEGFDVR 118 PGCPVC+ P++ I +E+ ++ +D+I+ ++GD+ R+P + S ++++EG DVR Sbjct: 68 PGCPVCVMPMQRIDLAIELAKR-----KDVIMCSYGDLLRVPGSKRKSLLNVRAEGADVR 122 Query: 119 VVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPP 178 +VYS D+ KIA+EN DK V+ F+ GFETTT TA +++ EG+ N + S H +TP Sbjct: 123 MVYSCLDTLKIAKENPDKKVIFFAIGFETTTPQTAVLIKKAKEEGIKNLFVVSNHVITPA 182 Query: 179 AVEALLKAGTV-------FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLL 231 A++ +L A + I PGHVS IIG + + Y E++ P V+AGFEP++VL Sbjct: 183 AIQHILNAPEIREIGKVEIDAFIGPGHVSVIIGTKPYHYFAEEFQKPVVIAGFEPLNVLK 242 Query: 232 GILILIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEV-KDAKWRALGIIPQSGLE 290 + ++++ + +G A++ N Y+R V ++GN+KAQ L+ + FE+ K+ +WR LG IP S L+ Sbjct: 243 AVYLILKQIYKGRAEVKNNYTRFVTYKGNLKAQNLVAELFELRKEFEWRGLGTIPYSALK 302 Query: 291 LKREWKDLEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCM 350 +++E+++ + ++ ++P+ P C+CG ++RG+A P C FG CTP +P+G CM Sbjct: 303 IRKEYEEFDAEKVFEVELPE-PKEHPACICGKVIRGVARPTDCKLFGTVCTPSNPLGSCM 361 Query: 351 VSYEGTCSIFYKY 363 VS EG C+ +YKY Sbjct: 362 VSSEGACAAYYKY 374 >sp|P31903|HYPD_ALCEU HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPD >gi|321749|pir||S29978 hypD protein - Alcaligenes eutrophus >gi|38775|emb|CAA49734.1| (X70183) HYPD [Ralstonia eutropha] Length = 379 Score = 284 bits (720), Expect = 9e-76 Identities = 147/372 (39%), Positives = 218/372 (58%), Gaps = 15/372 (4%) Query: 2 LEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPG 61 +E+FRD E+AQ+I + EA+ FM CG H ++R G+ LLPENV+++ GPG Sbjct: 4 IEEFRDGELAQRIAAHVRAEARPGQRYNFMEFCGGHTHAISRYGVTELLPENVRMIHGPG 63 Query: 62 CPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMG-SFADLKSEGFDVRVV 120 CPVC+ P+ I + + + D I+ T+GD R+P G S K+ G D+R+V Sbjct: 64 CPVCVLPIGRIDLALHLALE-----RDAIVCTYGDTMRVPASGGMSLIRAKAHGADIRMV 118 Query: 121 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV 180 YS D+ KIAQ + + VV + GFETTT PTA ++ +DNF + H LTP A+ Sbjct: 119 YSAADALKIAQRHPQREVVFLAIGFETTTPPTALIIREAKARQVDNFSVLCCHVLTPSAI 178 Query: 181 EALLKA------GTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGI 233 +L++ GTV G + P HVS +IG R + + + +Y P V+AGFEP+DV+ I Sbjct: 179 THILESPEVRDYGTVPIDGFVGPAHVSIVIGTRPYEHFSREYGKPVVIAGFEPLDVMQAI 238 Query: 234 LILIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDA-KWRALGIIPQSGLELK 292 L+L+R V G A++ NE+ R V +GN AQ ++++ FE++ + +WR LG +P S L ++ Sbjct: 239 LMLVRQVNSGRAEVENEFVRAVTRDGNESAQAMVSEVFELRPSFEWRGLGEVPYSALRIR 298 Query: 293 REWKDLEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVS 352 ++ + ++ +PD K C CGAILRG+ P C F CTP +P+G CMVS Sbjct: 299 AQFARFDAEQRFDLRYRPVPD-NKACECGAILRGVKKPTDCKLFATVCTPENPMGSCMVS 357 Query: 353 YEGTCSIFYKYG 364 EG C+ Y YG Sbjct: 358 SEGACAAHYSYG 369 >sp|P42033|HUPD_AZOCH HYDROGENASE EXPRESSION/FORMATION PROTEIN HUPD >gi|2120752|pir||I39743 hydrogenase homolog 1 - Azotobacter chroococcum >gi|142321|gb|AAA22136.1| (M92282) hydrogenase-related protein [Azotobacter chroococcum] >gi|382215|prf||1819270A hydrogenase [Azotobacter chroococcum] Length = 379 Score = 279 bits (707), Expect = 3e-74 Identities = 148/371 (39%), Positives = 223/371 (59%), Gaps = 14/371 (3%) Query: 2 LEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPG 61 +++FRD A+++ +I EA + R M CG H + R GI LLP +V+++ GPG Sbjct: 4 VDEFRDGATARQLAARIAAEADPARQYRLMEFCGGHTHAIFRYGIPDLLPASVRLIHGPG 63 Query: 62 CPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTP-MGSFADLKSEGFDVRVV 120 CPVC+ P+ + +E+ R+ ++IL T+GDM R+P S ++E VR++ Sbjct: 64 CPVCVMPIGRLDMAIELARRP-----EVILCTYGDMLRVPASGRVSLLKARAESAAVRML 118 Query: 121 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV 180 YS ++ K+AQ+N K VV F+ GFETTT PTA ++E GL NF ++ H LTP A+ Sbjct: 119 YSPAEALKLAQDNPSKEVVFFAIGFETTTPPTALVIEQAQRLGLKNFSVFCNHVLTPAAM 178 Query: 181 EALL-----KAGTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGIL 234 A+L +AGT+ G I P HVST+IG +A ++Y P V+AGFEP+D+L +L Sbjct: 179 HAILATPEARAGTLQLDGFIGPAHVSTVIGSAPYAPFPQQYGTPLVIAGFEPLDLLQALL 238 Query: 235 ILIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDA-KWRALGIIPQSGLELKR 293 +L+R + EG A++ N+++R V EGN KA+ L + FEV+++ +WR LG +P S L+++ Sbjct: 239 MLVRQLNEGRAEVENQFTRAVTAEGNRKAKALTERVFEVRESFEWRGLGAVPHSALQIRE 298 Query: 294 EWKDLEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSY 353 + + + + P+ K C C AILRG P C FG CTP +P+G CMVS Sbjct: 299 AFAEFNAERRFALE-PRTGLENKACECPAILRGAKSPRDCKLFGNPCTPDNPLGSCMVSS 357 Query: 354 EGTCSIFYKYG 364 EG C+ +Y YG Sbjct: 358 EGACAAWYAYG 368 >sp|P31882|HYPD_AZOVI HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPD >gi|95053|pir||S23443 hypothetical protein 8 - Azotobacter vinelandii >gi|39245|emb|CAA45187.1| (X63650) ORF8 [Azotobacter vinelandii] >gi|398011|gb|AAA19512.1| (L23970) hypD [Azotobacter vinelandii] Length = 379 Score = 277 bits (700), Expect = 2e-73 Identities = 146/371 (39%), Positives = 222/371 (59%), Gaps = 14/371 (3%) Query: 2 LEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPG 61 +++FRD A+++ +I EA + R M CG H + R GI LLP NV+++ GPG Sbjct: 4 VDEFRDGATARQLAARIAAEADPKRQYRLMEFCGGHTHAIFRYGIPDLLPANVRLIHGPG 63 Query: 62 CPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTP-MGSFADLKSEGFDVRVV 120 CPVC+ P+ + +E+ R+ +IL T+GDM R+P S ++EG VR++ Sbjct: 64 CPVCVMPIGRLDMAIELARRP-----GVILCTYGDMLRVPASGRVSLLKARAEGAAVRML 118 Query: 121 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV 180 YS ++ K+AQ+N D+ VV F+ GFETTT PTA ++E GL NF ++ H LTP A+ Sbjct: 119 YSPIEALKLAQDNPDRDVVFFAIGFETTTPPTALVIEQAQRLGLKNFSVFCNHVLTPAAM 178 Query: 181 EALL-----KAGTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGIL 234 A+L +AGT+ G I P HVST+IG +A +Y P V+AGFEP+D+L +L Sbjct: 179 HAILATPEARAGTLQLDGFIGPAHVSTVIGSDAYAPFPRQYGTPLVIAGFEPLDLLQALL 238 Query: 235 ILIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDA-KWRALGIIPQSGLELKR 293 +L+R + EG A++ N+++R V GN+KA+ L + FE++++ +WR LG +P S L ++ Sbjct: 239 MLVRQLNEGRAEVENQFTRAVTAGGNLKAKALTGRIFELRESFEWRGLGALPNSALRIRE 298 Query: 294 EWKDLEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSY 353 + + + + + + K C C AILRG P C FG C+P +P+G CMVS Sbjct: 299 AFAEFDAERRFALEARSGLE-NKACECPAILRGAKSPRDCKLFGNPCSPDNPLGSCMVSS 357 Query: 354 EGTCSIFYKYG 364 EG C+ +Y YG Sbjct: 358 EGACAAWYAYG 368 >pir||H81410 hydrogenase isoenzymes formation protein Cj0625 [imported] - Campylobacter jejuni (strain NCTC 11168) >gi|6968092|emb|CAB75261.1| (AL139075) hydrogenase isoenzymes formation protein [Campylobacter jejuni] Length = 363 Score = 273 bits (690), Expect = 3e-72 Identities = 146/363 (40%), Positives = 214/363 (58%), Gaps = 9/363 (2%) Query: 2 LEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPG 61 +++FRDKE + + I +E K + + M +CG H ++ + + S+LP+ + + GPG Sbjct: 4 IDEFRDKESILALKKLIEQELK--NPINIMEICGGHTHSIMKYALPSILPKEINFIHGPG 61 Query: 62 CPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRVVY 121 CPVC+ P VR+ ++ A + D I T GD+ R+P S DL+++G DVR +Y Sbjct: 62 CPVCVMPR---VRIDTAIKLASMK--DTIFCTLGDLLRVPGSEISLLDLRAKGADVRALY 116 Query: 122 SIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAVE 181 S + +IA++N +K ++ F+ GFETTT +A +L+ VI E ++N + H P VE Sbjct: 117 SPLEVLEIAKQNLNKNIIFFAIGFETTTPMSALLLQKVIEEKINNVFFHINHITVPAPVE 176 Query: 182 ALLKAGTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMV 240 A++ V + + P HVS I G + L K+ P V+GFEPVD+L +L +I+ Sbjct: 177 AIMNDENVKINAFLGPSHVSVITGYGIYEPLAAKFKTPIAVSGFEPVDILESVLNIIKQS 236 Query: 241 KEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWKDLEI 300 EG K+ N+Y R V EGNVKAQ L+ K+F V D ++R LG+I GLELK E+ + Sbjct: 237 NEGTFKVYNQYKRAVSKEGNVKAQNLVKKYFRVCDFEFRGLGLIKDGGLELKEEFSAYDA 296 Query: 301 RTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIF 360 ++ V + K C+CG ILRGLA P C FGK CTPR PIG CMVS EG C+ + Sbjct: 297 SKKFDCTVQSKNE-SKACICGQILRGLAKPYDCKVFGKACTPRSPIGSCMVSGEGACAAY 355 Query: 361 YKY 363 YKY Sbjct: 356 YKY 358 >sp|P40598|HYPD_RHILV HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPD >gi|420900|pir||S32877 hypD protein - Rhizobium leguminosarum bv. viciae >gi|48736|emb|CAA37163.1| (X52974) hypD [Rhizobium leguminosarum] Length = 385 Score = 272 bits (688), Expect = 5e-72 Identities = 146/372 (39%), Positives = 212/372 (56%), Gaps = 15/372 (4%) Query: 2 LEKFRDKEIAQKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSGPG 61 ++++RD +A+ + R+I A FM CG H ++R G+ LLP NV+++ GPG Sbjct: 4 IDEYRDGSLAKDVARQITALADPARSYHFMEFCGGHTHAISRYGLEDLLPANVRMIHGPG 63 Query: 62 CPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGS-FADLKSEGFDVRVV 120 CPVC+ P+ I + + + +I L T+GD+ R+P GS K+ G D+R+V Sbjct: 64 CPVCVLPIGRIDAAIALAMRP-----EITLCTYGDLMRVPGSNGSSLLKAKAAGADIRMV 118 Query: 121 YSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPAV 180 YS D+ +IA D+ VV F+ GFETTT TA L+ I GL NF I+ H LTP + Sbjct: 119 YSTLDALRIADAEPDREVVFFAIGFETTTPTTALALKAAIARGLGNFSIFCNHVLTPAPI 178 Query: 181 EALL------KAGTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGI 233 + +L K GT+ G I P HVST+IG + + E++ P VVAGFEP+DV+ I Sbjct: 179 QNILESPDVRKLGTIKIDGFIGPAHVSTVIGTEPYEFFAEEFRKPVVVAGFEPLDVIQAI 238 Query: 234 LILIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDA-KWRALGIIPQSGLELK 292 L+L+R V +G ++ N+Y R V GN KAQ + FFE++++ +WR LG +P L L+ Sbjct: 239 LMLVRQVNDGRHEVENQYIRAVTALGNEKAQAEVANFFELRESFEWRGLGEVPYGALRLR 298 Query: 293 REWKDLEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVS 352 ++ + ++ P D C CGAILRG+ P C FG CTP P+G CMVS Sbjct: 299 PQYAAYDAEKRFSMVTPAAKD-NPACECGAILRGVKKPADCKLFGTVCTPDTPMGSCMVS 357 Query: 353 YEGTCSIFYKYG 364 EG C+ + YG Sbjct: 358 PEGACAAHWAYG 369 >sp|P31904|HYPD_BRAJA HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPD >gi|486752|pir||S35229 hypD' protein - Bradyrhizobium japonicum (fragment) >gi|39535|emb|CAA78988.1| (Z17373) hypD' [Bradyrhizobium japonicum] >gi|448282|prf||1916405A hypD gene [Bradyrhizobium japonicum] Length = 347 Score = 271 bits (685), Expect = 1e-71 Identities = 136/340 (40%), Positives = 206/340 (60%), Gaps = 15/340 (4%) Query: 34 CGTHEDTVTRAGIRSLLPENVKIMSGPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTT 93 CG H ++R G+ +LP NV+++ GPGCPVC+ P I + + + DIIL Sbjct: 3 CGGHTHAISRYGLEDMLPANVRMIHGPGCPVCVLPAGRIDMAIRLAMRP-----DIILCV 57 Query: 94 FGDMYRIPTPMG-SFADLKSEGFDVRVVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPT 152 +GD+ R+P G S K+ G D+R+VYS D+ +IA++N + VV F+ GFETTT PT Sbjct: 58 YGDLMRVPGSQGASLLKAKARGADIRMVYSTIDAIRIAEDNPGREVVFFAIGFETTTPPT 117 Query: 153 AGMLETVINEGLDNFKIYSVHRLTPPAVEALLKAGTV-------FHGLIDPGHVSTIIGV 205 A M+ + L+NF ++ H LTPPA++ +L++ + G + P HVSTIIG Sbjct: 118 AVMIRLAGKKQLENFSVFCNHVLTPPAMQNILESPDIRNIGRVEIDGFVGPAHVSTIIGT 177 Query: 206 RGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMVKEGEAKIINEYSRVVKWEGNVKAQE 265 + + E++ P V+AGFEP+D++ IL+L+R V E ++ N+YSR V +GN++A+E Sbjct: 178 APYEFFAEEFGKPVVIAGFEPLDMMQAILMLVRQVNEHRHEVENQYSRAVTRDGNLRAKE 237 Query: 266 LINKFFEVKDA-KWRALGIIPQSGLELKREWKDLEIRTLYNPDVPKLPDLEKGCLCGAIL 324 ++ FE++D +WR LG +P SGL+LKR + + ++ + ++ D C CGAIL Sbjct: 238 EVSDIFELRDQFEWRGLGQVPYSGLKLKRAYAKYDAEVRFDMNELRVDD-NPACECGAIL 296 Query: 325 RGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIFYKYG 364 RG+ P C FG CTP P+G CMVS EG C+ + YG Sbjct: 297 RGVKKPVDCKLFGTVCTPETPMGSCMVSSEGACAAHWTYG 336 >pir||S74470 hydrogenase isoenzymes formation protein hypD - Synechocystis sp. (strain PCC 6803) >gi|1651694|dbj|BAA16622.1| (D90899) hydrogenase isoenzymes formation protein HypD [Synechocystis sp.] Length = 374 Score = 262 bits (663), Expect = 4e-69 Identities = 140/371 (37%), Positives = 211/371 (56%), Gaps = 22/371 (5%) Query: 2 LEKFRDKEIA----QKIVRKIHKEAKGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIM 57 ++++RD + Q I R+I K M +CG ++ + G+ +LLP+N+ ++ Sbjct: 4 VDEYRDAQAVAHYRQAIAREITKP------WTLMEICGGQTHSIVKYGLDALLPKNLTLI 57 Query: 58 SGPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDV 117 GPGCPVC+TP+E I + + + ++ +II +FGDM R+P +K++G DV Sbjct: 58 HGPGCPVCVTPMELIDQALWLAKQP-----EIIFCSFGDMLRVPGSGADLLSIKAQGGDV 112 Query: 118 RVVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTP 177 R+VYS D IA+EN ++ VV F GFETT TA L +G+ NF + H L P Sbjct: 113 RIVYSPLDCLAIARENPNREVVFFGVGFETTAPATAMTLHQARAQGISNFSLLCAHVLVP 172 Query: 178 PAVEALL-KAGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILIL 236 PA+EALL ++ G + GHV T+ G R + ++ EKY +P V+ GFEPVD++ GI Sbjct: 173 PAMEALLGNPNSLVQGFLAAGHVCTVTGERAYQHIAEKYQVPIVITGFEPVDIMQGIFAC 232 Query: 237 IRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDAKWRALGIIPQSGLELKREWK 296 +R ++ G+ N+Y R V+ +GN AQ++I++ FE D WR LG+IP SGL L+ + Sbjct: 233 VRQLESGQFTCNNQYRRSVQPQGNAHAQKIIDQVFEPVDRHWRGLGLIPASGLGLRPAFA 292 Query: 297 D----LEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVS 352 ++ L P + E C+ G IL+G P CP FG CTP P+G MVS Sbjct: 293 PWDAAVKFANLLQTMAPTMG--ETVCISGEILQGQRKPSDCPAFGTICTPEQPLGAPMVS 350 Query: 353 YEGTCSIFYKY 363 EG C+ +Y+Y Sbjct: 351 SEGACAAYYRY 361 >pir||B64632 hydrogenase expression/formation protein - Helicobacter pylori (strain 26695) >gi|2314033|gb|AAD07944.1| (AE000599) hydrogenase expression/formation protein (hypD) [Helicobacter pylori 26695] Length = 370 Score = 261 bits (661), Expect = 7e-69 Identities = 141/367 (38%), Positives = 212/367 (57%), Gaps = 9/367 (2%) Query: 1 MLEKFRDKEIAQKIVRKIHKEAKGFDE-LRFMHVCGTHEDTVTRAGIRSLLPENVKIMSG 59 ++ FRDK + I K A ++ L M VCG H ++ + G+ L+P N++ + G Sbjct: 6 LISPFRDKRTLLALSNAIKKLAFKLEKKLVIMEVCGGHTHSIMKYGLLDLMPNNLEFVHG 65 Query: 60 PGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRV 119 PGCPVC+ P + E+ +D I+ + GDM R+P GS + +G D R Sbjct: 66 PGCPVCVMPRARLDEAYELATI-----KDSIVLSLGDMMRVPGSYGSLIQAREKGLDARF 120 Query: 120 VYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPA 179 +YS + +IA+EN K V++ + GFETTT +A +L + E ++N + H L PP+ Sbjct: 121 LYSPMQALEIAKENPTKKVIYIAIGFETTTPMSASVLWSAKKEKINNLFFHINHILVPPS 180 Query: 180 VEALLK-AGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIR 238 V A+LK + L+ P HVS I G + +A L +++ IP +V+GFEPVD+L +L+LI+ Sbjct: 181 VSAILKDPACQINALLAPSHVSVISGAQIYAPLVDRFKIPIIVSGFEPVDILESVLMLIK 240 Query: 239 MVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKD-AKWRALGIIPQSGLELKREWKD 297 EAK+ +Y R V +EGNVKAQEL+N EV++ +WR LG I +S L+LK + Sbjct: 241 QALNKEAKLEIQYKRAVSFEGNVKAQELVNACMEVRENFEWRGLGNIKRSALKLKEAFAS 300 Query: 298 LEIRTLYNPDVP-KLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGT 356 + ++ + K K C CG IL+G+A P C F TCTP++PIG CMVS EG Sbjct: 301 YDAEEVFKEYLSHKTSKENKACKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGA 360 Query: 357 CSIFYKY 363 C+ +Y+Y Sbjct: 361 CAAYYRY 367 >pir||F71883 hydrogenase expression/formation protein - Helicobacter pylori (strain J99) >gi|4155404|gb|AAD06413.1| (AE001512) Hydrogenase expression/formation protein [Helicobacter pylori J99] Length = 370 Score = 259 bits (654), Expect = 5e-68 Identities = 140/367 (38%), Positives = 209/367 (56%), Gaps = 9/367 (2%) Query: 1 MLEKFRDKEIAQKIVRKIHKEA-KGFDELRFMHVCGTHEDTVTRAGIRSLLPENVKIMSG 59 ++ FRDK I + +I K A K +L M VCG H ++ + G+ L+P+N++ G Sbjct: 6 LISPFRDKRIILALSNEIKKFASKLTKKLVIMEVCGGHTHSIMKYGLLDLMPKNLEFAHG 65 Query: 60 PGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVRV 119 PGCPVC+ P + E+ +D I+ + GDM R+P GS + +G D R Sbjct: 66 PGCPVCVMPRARLDEAYELASM-----KDSIVLSLGDMMRVPGSYGSLIQAREKGLDARF 120 Query: 120 VYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPPA 179 +YS + +IA+EN K V++ + GFETTT TA +L E L N + H L PP+ Sbjct: 121 LYSPMQALEIAKENPHKKVIYIAIGFETTTPMTASVLLNAKKEKLKNLFFHINHILVPPS 180 Query: 180 VEALLKAGTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIR 238 V +L+ + L+ P HVS I G + +A L +++ +P +V+GFEPVD+L +L+L + Sbjct: 181 VSTILEDKACRINALLAPSHVSVISGAQIYAPLVDRFKLPIIVSGFEPVDILESVLMLTK 240 Query: 239 MVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKD-AKWRALGIIPQSGLELKREWKD 297 EAK+ +Y R V +EGN KAQEL+N EV++ +WR LG I +S L+LK + Sbjct: 241 QALNKEAKLEIQYKRAVSYEGNTKAQELVNACMEVRENFEWRGLGNIKRSALKLKEAFAS 300 Query: 298 LEIRTLYNPDVP-KLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGT 356 + ++ + K K C CG IL+G+A P C F TCTP++PIG CMVS EG Sbjct: 301 YDAEKVFKAYLSHKTSKENKACKCGEILKGIAKPLDCSLFATTCTPQNPIGSCMVSSEGA 360 Query: 357 CSIFYKY 363 C+ +Y+Y Sbjct: 361 CAAYYRY 367 >gb|AAA69239.1| (U29579) CG Site no. 33116; ORF_o373 [Escherichia coli] Length = 373 Score = 243 bits (614), Expect = 2e-63 Identities = 133/369 (36%), Positives = 200/369 (54%), Gaps = 13/369 (3%) Query: 2 LEKFRDKEIAQKIVRKIHKEAKGFD-----ELRFMHVCGTHEDTVTRAGIRSLLPENVKI 56 ++++R E +++ + + A LR M VCG H + + G+ LLPENV+ Sbjct: 4 VDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPENVEF 63 Query: 57 MSGPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFD 116 + GPGCPVC+ P+ I +EI ++I TFGD R+P GS K+ G D Sbjct: 64 IHGPGCPVCVLPMGRIDTCVEIASHP-----EVIFCTFGDAMRVPGKQGSLLQAKARGAD 118 Query: 117 VRVVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLT 176 VR+VYS D+ K+AQEN + VV F GFETT TA L+ + NF + H Sbjct: 119 VRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARDVQNFYFFCQHITL 178 Query: 177 PPAVEALL-KAGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILI 235 P + +LL + + PGHVS +IG + ++ ++ P VVAGFEP+D+L G+++ Sbjct: 179 IPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVM 238 Query: 236 LIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVK-DAKWRALGIIPQSGLELKRE 294 L++ +K+ N+Y RVV GN+ AQ+ I F V D++WR LG+I SG+ L + Sbjct: 239 LVQQKIAAHSKVENQYRRVVPDAGNLLAQQAIXDVFCVNGDSEWRGLGVIESSGVHLTPD 298 Query: 295 WKDLEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYE 354 ++ + + P ++ D + CG +L G P QCP FG TC P+ G MVS E Sbjct: 299 YQRFDAEAHFRPAPQQVCD-DPRARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSE 357 Query: 355 GTCSIFYKY 363 G C+ +Y+Y Sbjct: 358 GACAAWYQY 366 >sp|P24192|HYPD_ECOLI HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPD >gi|95944|pir||S15200 hydrogenase expression/formation protein HypD - Escherichia coli >gi|41778|emb|CAA38415.1| (X54543) hydrogenase isoenzyme hypD [Escherichia coli] >gi|1789084|gb|AAC75771.1| (AE000356) pleiotrophic effects on 3 hydrogenase isozymes [Escherichia coli K12] Length = 373 Score = 243 bits (613), Expect = 3e-63 Identities = 133/369 (36%), Positives = 200/369 (54%), Gaps = 13/369 (3%) Query: 2 LEKFRDKEIAQKIVRKIHKEAKGFD-----ELRFMHVCGTHEDTVTRAGIRSLLPENVKI 56 ++++R E +++ + + A LR M VCG H + + G+ LLPENV+ Sbjct: 4 VDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPENVEF 63 Query: 57 MSGPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFD 116 + GPGCPVC+ P+ I +EI ++I TFGD R+P GS K+ G D Sbjct: 64 IHGPGCPVCVLPMGRIDTCVEIASHP-----EVIFCTFGDAMRVPGKQGSLLQAKARGAD 118 Query: 117 VRVVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLT 176 VR+VYS D+ K+AQEN + VV F GFETT TA L+ + NF + H Sbjct: 119 VRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARDVQNFYFFCQHITL 178 Query: 177 PPAVEALL-KAGTVFHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILI 235 P + +LL + + PGHVS +IG + ++ ++ P VVAGFEP+D+L G+++ Sbjct: 179 IPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVM 238 Query: 236 LIRMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVK-DAKWRALGIIPQSGLELKRE 294 L++ +K+ N+Y RVV GN+ AQ+ I F V D++WR LG+I SG+ L + Sbjct: 239 LVQQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPD 298 Query: 295 WKDLEIRTLYNPDVPKLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYE 354 ++ + + P ++ D + CG +L G P QCP FG TC P+ G MVS E Sbjct: 299 YQRFDAEAHFRPAPQQVCD-DPRARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSE 357 Query: 355 GTCSIFYKY 363 G C+ +Y+Y Sbjct: 358 GACAAWYQY 366 >pir||S59984 hypD protein - Rhodobacter capsulatus >gi|46055|emb|CAA43328.1| (X61007) HypD [Rhodobacter capsulatus] Length = 473 Score = 236 bits (597), Expect = 2e-61 Identities = 129/371 (34%), Positives = 200/371 (53%), Gaps = 15/371 (4%) Query: 4 KFRDKEIAQKIVRKIHKEAKGFDE-----LRFMHVCGTHEDTVTRAGIRSLLPENVKIMS 58 +FRD +A+ ++ +I + A + M +CG H ++ R G+ L+ ++ + Sbjct: 102 EFRDPALAKGLLAEIARLADQIGATAEKPVHIMEICGGHTHSIFRYGLDKLIHPGIEFIH 161 Query: 59 GPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVR 118 GPGCPVC+ P + +EI + ++I TFGD R+P S K+ G D+R Sbjct: 162 GPGCPVCVLPRARVDECIEIAGRP-----EVIFCTFGDAMRVPGSKLSLMQAKAAGADIR 216 Query: 119 VVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPP 178 +VYS D+ ++A+ N + VV F GFETTT TA ++ EGL NF ++ H P Sbjct: 217 MVYSPLDALELARRNPGREVVFFGLGFETTTPSTALAIQQAAREGLANFSVFCNHITVPE 276 Query: 179 AVEALLKAGTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILI 237 + ALL + G I PGHVS +IG+ + ++ E Y P VVAGFEP D+L +L+++ Sbjct: 277 PIRALLDDPYMRLDGFIGPGHVSMVIGIHPYDFIAEDYGKPLVVAGFEPTDLLQSVLMVL 336 Query: 238 RMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDA-KWRALGIIPQSGLELKREWK 296 R + +G A I N+Y+RVV GN + I +E + + +WR LG I SGL ++ ++ Sbjct: 337 RQISQGRAAIENQYARVVPEHGNRVSLAAIADVYERRPSFEWRGLGEIDASGLRIRAAYR 396 Query: 297 DLEIRTLYNPDVP---KLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSY 353 + + + +GC CGA++ G P C FGK CTP P+G MVS Sbjct: 397 AHDAEEKFGVGYAGQRAAVEEAEGCACGAVMTGRMKPVACAQFGKGCTPEMPLGALMVSS 456 Query: 354 EGTCSIFYKYG 364 EG C+ +++YG Sbjct: 457 EGACAAYWQYG 467 >sp|P26411|HYPD_RHOCA HYDROGENASE EXPRESSION/FORMATION PROTEIN HYPD >gi|2126467|pir||S59985 hypD protein - Rhodobacter capsulatus Length = 377 Score = 236 bits (597), Expect = 2e-61 Identities = 129/371 (34%), Positives = 200/371 (53%), Gaps = 15/371 (4%) Query: 4 KFRDKEIAQKIVRKIHKEAKGFDE-----LRFMHVCGTHEDTVTRAGIRSLLPENVKIMS 58 +FRD +A+ ++ +I + A + M +CG H ++ R G+ L+ ++ + Sbjct: 6 EFRDPALAKGLLAEIARLADQIGATAEKPVHIMEICGGHTHSIFRYGLDKLIHPGIEFIH 65 Query: 59 GPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVR 118 GPGCPVC+ P + +EI + ++I TFGD R+P S K+ G D+R Sbjct: 66 GPGCPVCVLPRARVDECIEIAGRP-----EVIFCTFGDAMRVPGSKLSLMQAKAAGADIR 120 Query: 119 VVYSIYDSYKIAQENRDKLVVHFSPGFETTTAPTAGMLETVINEGLDNFKIYSVHRLTPP 178 +VYS D+ ++A+ N + VV F GFETTT TA ++ EGL NF ++ H P Sbjct: 121 MVYSPLDALELARRNPGREVVFFGLGFETTTPSTALAIQQAAREGLANFSVFCNHITVPE 180 Query: 179 AVEALLKAGTV-FHGLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILI 237 + ALL + G I PGHVS +IG+ + ++ E Y P VVAGFEP D+L +L+++ Sbjct: 181 PIRALLDDPYMRLDGFIGPGHVSMVIGIHPYDFIAEDYGKPLVVAGFEPTDLLQSVLMVL 240 Query: 238 RMVKEGEAKIINEYSRVVKWEGNVKAQELINKFFEVKDA-KWRALGIIPQSGLELKREWK 296 R + +G A I N+Y+RVV GN + I +E + + +WR LG I SGL ++ ++ Sbjct: 241 RQISQGRAAIENQYARVVPEHGNRVSLAAIADVYERRPSFEWRGLGEIDASGLRIRAAYR 300 Query: 297 DLEIRTLYNPDVP---KLPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSY 353 + + + +GC CGA++ G P C FGK CTP P+G MVS Sbjct: 301 AHDAEEKFGVGYAGQRAAVEEAEGCACGAVMTGRMKPVACAQFGKGCTPEMPLGALMVSS 360 Query: 354 EGTCSIFYKYG 364 EG C+ +++YG Sbjct: 361 EGACAAYWQYG 371 >gb|AAB64435.1| (AF006594) HupD [Anabaena PCC7120] Length = 199 Score = 146 bits (365), Expect = 4e-34 Identities = 71/174 (40%), Positives = 106/174 (60%), Gaps = 2/174 (1%) Query: 192 GLIDPGHVSTIIGVRGWAYLTEKYNIPQVVAGFEPVDVLLGILILIRMVKEGEAKIINEY 251 G I PGHVS +IG + ++ ++Y+ P VV+GFEP+D+ I +L++ + E ++ N+Y Sbjct: 7 GFIGPGHVSMVIGTEPYEFIAQQYHKPIVVSGFEPLDIFQSIWMLLQQLVENRCEVENQY 66 Query: 252 SRVVKWEGNVKAQELINKFFEVKDA-KWRALGIIPQSGLELKREWKDLEIRTLYNPDVPK 310 +R+V+ GN A ++K F V++ WR L IP SGL+++ E+ + + K Sbjct: 67 NRLVQKGGNQIALAAMHKVFAVREKFAWRGLDEIPDSGLKIREEYAQFDAELKFTIPNLK 126 Query: 311 LPDLEKGCLCGAILRGLALPPQCPHFGKTCTPRHPIGPCMVSYEGTCSIFYKYG 364 + D K C CG IL+G+ P QC FG CTP PIG CMVS EG C+ +YKYG Sbjct: 127 VAD-HKACKCGEILKGVPQPWQCKVFGTACTPETPIGTCMVSSEGACAAYYKYG 179 >gb|AAF20001.1|AF214145_8 (AF214145) hydrogenase maturating protein HypD [Rhodobacter sphaeroides] Length = 123 Score = 69.5 bits (167), Expect = 6e-11 Identities = 38/123 (30%), Positives = 60/123 (47%), Gaps = 10/123 (8%) Query: 4 KFRDKEIAQKIVRKIHKEAKGFDELR-----FMHVCGTHEDTVTRAGIRSLLPENVKIMS 58 +FRD +A+ ++ I A R M +CG H + R G+ L+ E ++ + Sbjct: 6 EFRDPALARSLLAAIAARADEMGATRARPLHIMEICGGHTHAIFRYGLDRLVHEGIEFIH 65 Query: 59 GPGCPVCITPVEDIVRMMEIMRKAKEEGEDIILTTFGDMYRIPTPMGSFADLKSEGFDVR 118 GPGCPVC+ P+ + + + I +ILTTF +P GS K+ G D+R Sbjct: 66 GPGCPVCVLPIPPLHQSIPI-----PHPPHLILTTFPHPIPLPGARGSLLQAKARGADIR 120 Query: 119 VVY 121 +VY Sbjct: 121 MVY 123 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.322 0.142 0.433 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 146479878 Number of Sequences: 2977 Number of extensions: 6442854 Number of successful extensions: 12846 Number of sequences better than 1.0e-10: 21 Number of HSP's better than 0.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 12754 Number of HSP's gapped (non-prelim): 21 length of query: 367 length of database: 189,106,746 effective HSP length: 52 effective length of query: 315 effective length of database: 157,985,422 effective search space: 49765407930 effective search space used: 49765407930 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.9 bits) S2: 166 (69.1 bits)