BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1576 (PAB1576) DE:PHOSPHOGLYCOLATE PHOSPHATASE (231 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||A75103 phosphoglycolate phosphatase PAB1576 - Pyrococcus ab... 460 e-129 pir||B71084 hypothetical protein PH0935 - Pyrococcus horikoshii ... 369 e-101 pir||D64479 hypothetical protein MJ1437 - Methanococcus jannasch... 80 2e-14 gb|AAK42335.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solf... 78 9e-14 pir||S74066 hypothetical protein c0135 - Sulfolobus solfataricus... 78 9e-14 pir||G71045 hypothetical protein PH1655 - Pyrococcus horikoshii ... 71 9e-12 pir||E75169 hypothetical protein PAB2019 - Pyrococcus abyssi (st... 71 2e-11 pir||E75005 probable haloacid dehalogenase (EC 3.8.1.-) PAB1316 ... 69 4e-11 >pir||A75103 phosphoglycolate phosphatase PAB1576 - Pyrococcus abyssi (strain Orsay) >gi|5458638|emb|CAB50126.1| (AJ248286) PHOSPHOGLYCOLATE PHOSPHATASE [Pyrococcus abyssi] Length = 231 Score = 460 bits (1171), Expect = e-129 Identities = 231/231 (100%), Positives = 231/231 (100%) Query: 1 MMLVILDLDDTLCNTWEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDLHLLD 60 MMLVILDLDDTLCNTWEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDLHLLD Sbjct: 1 MMLVILDLDDTLCNTWEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDLHLLD 60 Query: 61 FKGIFNRIMEDIYEDVDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLKAKIVLVT 120 FKGIFNRIMEDIYEDVDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLKAKIVLVT Sbjct: 61 FKGIFNRIMEDIYEDVDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLKAKIVLVT 120 Query: 121 DSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMFPREEIVFVVGDRDE 180 DSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMFPREEIVFVVGDRDE Sbjct: 121 DSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMFPREEIVFVVGDRDE 180 Query: 181 TDMKGGKSIGAITILVRRGYFKGRRVRYADHVVNDLYEALEVIKNELKARA 231 TDMKGGKSIGAITILVRRGYFKGRRVRYADHVVNDLYEALEVIKNELKARA Sbjct: 181 TDMKGGKSIGAITILVRRGYFKGRRVRYADHVVNDLYEALEVIKNELKARA 231 >pir||B71084 hypothetical protein PH0935 - Pyrococcus horikoshii >gi|3257349|dbj|BAA30032.1| (AP000004) 246aa long hypothetical protein [Pyrococcus horikoshii] Length = 246 Score = 369 bits (937), Expect = e-101 Identities = 172/229 (75%), Positives = 207/229 (90%) Query: 1 MMLVILDLDDTLCNTWEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDLHLLD 60 +MLVILDLDDTLCNTWEALR+A+++L P II RKF+LIAYF+TKRYE+LE +RD+HLLD Sbjct: 18 LMLVILDLDDTLCNTWEALRLALIRLTPIIIMKRKFKLIAYFLTKRYEKLESIRDIHLLD 77 Query: 61 FKGIFNRIMEDIYEDVDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLKAKIVLVT 120 F+GIFNRIMEDIY D+D+EE+KE+L LFDR FF++LKLY+D FL ELK +KAK+ LVT Sbjct: 78 FEGIFNRIMEDIYRDLDKEEIKEMLDLFDRAFFANLKLYDDVLPFLNELKQMKAKLALVT 137 Query: 121 DSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMFPREEIVFVVGDRDE 180 DSASSWQR+KLEVLGI+ YFD +IVSGDTGHTKLEPYNF LA +MFP+E+ +FV+GDRD+ Sbjct: 138 DSASSWQRRKLEVLGIQRYFDKIIVSGDTGHTKLEPYNFYLATKMFPKEDKIFVIGDRDD 197 Query: 181 TDMKGGKSIGAITILVRRGYFKGRRVRYADHVVNDLYEALEVIKNELKA 229 TDMKGGK+IGA TILV+RGYFKGRR +YAD++VN+LYEALEVIK ELKA Sbjct: 198 TDMKGGKAIGATTILVKRGYFKGRRAKYADYIVNNLYEALEVIKGELKA 246 >pir||D64479 hypothetical protein MJ1437 - Methanococcus jannaschii >gi|1592085|gb|AAB99446.1| (U67584) L-2-haloalkanoic acid dehalogenase isolog [Methanococcus jannaschii] Length = 228 Score = 80.4 bits (195), Expect = 2e-14 Identities = 64/229 (27%), Positives = 113/229 (48%), Gaps = 16/229 (6%) Query: 4 VILDLDDTLCNTWEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDLHLLDFKG 63 ++ DLDDTL N+ E + IA + V ++I + +E + + + D Sbjct: 5 ILFDLDDTLYNSSEFVEIARREAVKSMIDAG--------LNIDFEEAMNILNKIIKDKGS 56 Query: 64 IFNRIMEDIYEDV----DREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLKAKIVLV 119 + + +D+ + V D + + + + + L+ Y L ELK + K+ ++ Sbjct: 57 NYGKHFDDLVKAVLGKYDPKIITTGIITYHNVKVALLRPYPHTIKTLMELKAMGLKLGVI 116 Query: 120 TDSASSWQRKKLEVLGIENYFDAVIVSGDTGHTK--LEPYNFKLAKRMFPREEIVFVVGD 177 TD + Q +KL LGI +FD VI S + G K LE + + L + EE V+ VGD Sbjct: 117 TDGLTIKQWEKLIRLGIHPFFDDVITSEEFGLGKPHLEFFKYGLKRMGLKAEETVY-VGD 175 Query: 178 RDETDMKGGKSIGAITILVRRGYFKGRR-VRYADHVVNDLYEALEVIKN 225 R + D+K K +G IT+ + +G +K Y+D+ +N L E ++++KN Sbjct: 176 RVDKDIKPAKELGMITVRILKGKYKDMEDDEYSDYTINSLQELVDIVKN 224 >gb|AAK42335.1| 2-haloalkanoic acid dehalogenase [Sulfolobus solfataricus] Length = 222 Score = 78.0 bits (189), Expect = 9e-14 Identities = 69/236 (29%), Positives = 117/236 (49%), Gaps = 24/236 (10%) Query: 1 MMLVILDLDDTLCNT----WEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDL 56 M + +DL +TL + E + A+ ++ + R F+ +A + K + ++ L Sbjct: 1 MKTIFVDLGETLVHFKPRYHENIAYALKEIGYNVDERRVFKAVAKILGKHHYPSQEYGGL 60 Query: 57 HLLDFKGIFNRIMEDIYED---VDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLK 113 DF+ +F + +IY D + R K +L +LY+D+ FL+E K L Sbjct: 61 SAFDFRELFYEL--NIYPDEQLITRLNSKNLLS-------GEYELYDDSITFLEEAKGLG 111 Query: 114 AKIVLVTDSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMFPREEIVF 173 K+VLV+++ S K +E LGI+ YFD ++ S D K P F A + + I Sbjct: 112 FKLVLVSNATRSIY-KIVEDLGIKKYFDGIVASCDLNIMKPHPKIFSYAMEIAKSDGI-- 168 Query: 174 VVGDRDETDMKGGKSIGAITILV-RRGYFKGRRVRYADHVVNDLYEALEVIKNELK 228 +GD E D+ G K G IL+ R G++ R ++ V++L EALE++K +L+ Sbjct: 169 HIGDIYEIDVIGAKRAGLEAILLDRLGFYPEIR----ENKVSNLLEALELVKEKLR 220 >pir||S74066 hypothetical protein c0135 - Sulfolobus solfataricus >gi|1707765|emb|CAA69437.1| (Y08256) orf c01035 [Sulfolobus solfataricus] Length = 222 Score = 78.0 bits (189), Expect = 9e-14 Identities = 69/236 (29%), Positives = 117/236 (49%), Gaps = 24/236 (10%) Query: 1 MMLVILDLDDTLCNT----WEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDL 56 M + +DL +TL + E + A+ ++ + R F+ +A + K + ++ L Sbjct: 1 MKTIFVDLGETLVHFKPRYHENIAYALKEIGYNVDERRVFKAVAKILGKHHYPSQEYGGL 60 Query: 57 HLLDFKGIFNRIMEDIYED---VDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLK 113 DF+ +F + +IY D + R K +L +LY+D+ FL+E K L Sbjct: 61 SAFDFRELFYEL--NIYPDEQLITRLNSKNLLS-------GEYELYDDSITFLEEAKGLG 111 Query: 114 AKIVLVTDSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMFPREEIVF 173 K+VLV+++ S K +E LGI+ YFD ++ S D K P F A + + I Sbjct: 112 FKLVLVSNATRSIY-KIVEDLGIKKYFDGIVASCDLNIMKPHPKIFSYAMEIAKSDGI-- 168 Query: 174 VVGDRDETDMKGGKSIGAITILV-RRGYFKGRRVRYADHVVNDLYEALEVIKNELK 228 +GD E D+ G K G IL+ R G++ R ++ V++L EALE++K +L+ Sbjct: 169 HIGDIYEIDVIGAKRAGLEAILLDRLGFYPEIR----ENKVSNLLEALELVKEKLR 220 >pir||G71045 hypothetical protein PH1655 - Pyrococcus horikoshii >gi|3258084|dbj|BAA30767.1| (AP000006) 241aa long hypothetical protein [Pyrococcus horikoshii] Length = 241 Score = 71.4 bits (172), Expect = 9e-12 Identities = 62/228 (27%), Positives = 111/228 (48%), Gaps = 8/228 (3%) Query: 3 LVILDLDDTLCNTWEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDLHLLDFK 62 ++ DLDDTL +T + IA + +IR L F T E +E +++ + +F Sbjct: 4 VIFFDLDDTLVDTSKLAEIARKNAIENMIR---HGLPVDFETAYSELIELIKE-YGSNFP 59 Query: 63 GIFNRIMEDIYEDVDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLKAKIVLVTDS 122 F+ ++ + + + + + + F+ L+ A L LK L ++ ++TD Sbjct: 60 YHFDYLLRRLDLPYNPKWISAGVIAYHNTKFAYLREVPGARKVLIRLKELGYELGIITDG 119 Query: 123 ASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMF-PREEIVFVVGDRDET 181 Q +K+ L ++++F+ VI+S G K P FK A + F + E +VGDR + Sbjct: 120 NPVKQWEKILRLELDDFFEHVIISDFEGVKKPHPKIFKKALKAFNVKPEEALMVGDRLYS 179 Query: 182 DMKGGKSIGAITILVRRGYFKGRRV---RYADHVVNDLYEALEVIKNE 226 D+ G K +G T+ R G R + +YAD+ +++L LEV+ E Sbjct: 180 DIYGAKRVGMKTVWFRYGKHSERELEYRKYADYEIDNLESLLEVLARE 227 >pir||E75169 hypothetical protein PAB2019 - Pyrococcus abyssi (strain Orsay) >gi|5457946|emb|CAB49436.1| (AJ248284) hypothetical protein [Pyrococcus abyssi] Length = 238 Score = 70.6 bits (170), Expect = 2e-11 Identities = 60/228 (26%), Positives = 112/228 (48%), Gaps = 8/228 (3%) Query: 3 LVILDLDDTLCNTWEALRIAVMKLVPTIIRMRKFRLIAYFITKRYERLEKLRDLHLLDFK 62 ++ DLDDTL +T + +A + +IR L F T E +E +++ + +F Sbjct: 4 VIFFDLDDTLVDTTKLAELARRNAIENMIR---HGLPVDFETAYSELMELIKE-YGSNFP 59 Query: 63 GIFNRIMEDIYEDVDREEVKEILKLFDRFFFSSLKLYEDAEYFLKELKNLKAKIVLVTDS 122 F+ ++ + + + V + + F+ L+ A L L+ L ++ ++TD Sbjct: 60 HHFDYLLRRLDLPYNPKWVSAGVIAYHNTKFAYLREVPGARKVLIRLRELGYRLGIITDG 119 Query: 123 ASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEPYNFKLAKRMFPRE-EIVFVVGDRDET 181 Q +K+ L I+++F+ VI+S G K P FK A + F + + +VGDR + Sbjct: 120 NPVKQWEKILRLEIDDFFEHVIISDFEGVKKPHPKIFKKALKAFNVDAQEALMVGDRLYS 179 Query: 182 DMKGGKSIGAITILVRRGYFKGRRV---RYADHVVNDLYEALEVIKNE 226 D+ G K++G T+ + G + + YAD+ + L + L+VI+NE Sbjct: 180 DIYGAKNVGMKTVWFKYGKYSKEELEYREYADYEIEKLQDLLKVIENE 227 >pir||E75005 probable haloacid dehalogenase (EC 3.8.1.-) PAB1316 [similarity] - Pyrococcus abyssi (strain Orsay) >gi|5458997|emb|CAB50483.1| (AJ248288) 2-HALOALKANOIC ACID DEHALOGENASE (EC 3.8.1.2 RELATED PROTEIN [Pyrococcus abyssi] Length = 229 Score = 69.1 bits (166), Expect = 4e-11 Identities = 57/191 (29%), Positives = 92/191 (47%), Gaps = 10/191 (5%) Query: 45 KRYERLEKLRDLHLLDFKGIFNRIMEDIYEDVDREEVKEI-LKLFDRFFFSSLK------ 97 K E EKL + G R + DI E++ R+ +E K + F+ LK Sbjct: 40 KILEEYEKLTREAFSAYAGKPYRPIRDIEEEIMRKISEEYGFKYPENFWEIHLKMHQEYG 99 Query: 98 -LYEDAEYFLKELKNLKAKIVLVTDSASSWQRKKLEVLGIENYFDAVIVSGDTGHTKLEP 156 LY + LKEL+ + ++TDS + + LE LG+ + FD++ S + G K P Sbjct: 100 KLYPEVVEVLKELRQ-SYHVGMITDSDTEYLNAHLEALGVRDLFDSITTSEEAGFFKPHP 158 Query: 157 YNFKLA-KRMFPREEIVFVVGDRDETDMKGGKSIGAITILVRRGYFKGRRVRYADHVVND 215 F++A ++ + E VGD D G K++G I+IL+ R K D VV+D Sbjct: 159 RIFEIALRKAGVKGEDAVYVGDNPVKDCGGAKNLGMISILLDRTGEKKDLWDRCDFVVSD 218 Query: 216 LYEALEVIKNE 226 L E ++++K + Sbjct: 219 LREVIKIVKGQ 229 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.326 0.144 0.407 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 81026491 Number of Sequences: 2977 Number of extensions: 3283162 Number of successful extensions: 11855 Number of sequences better than 1.0e-10: 8 Number of HSP's better than 0.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 11837 Number of HSP's gapped (non-prelim): 8 length of query: 231 length of database: 189,106,746 effective HSP length: 54 effective length of query: 177 effective length of database: 156,788,448 effective search space: 27751555296 effective search space used: 27751555296 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 40 (21.6 bits) S2: 164 (68.3 bits)