BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1578 (PAB1578) DE:GROUP II DECARBOXYLASE
(384 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G75102 group II decarboxylase PAB1578 - Pyrococcus abyssi (... 773 0.0
pir||D71084 hypothetical protein PH0937 - Pyrococcus horikoshii ... 708 0.0
pir||E69015 glutamate decarboxylase - Methanobacterium thermoaut... 359 3e-98
gi|11499586 group II decarboxylase [Archaeoglobus fulgidus] >gi|... 344 1e-93
sp|Q60358|Y050_METJA HYPOTHETICAL PROTEIN MJ0050 >gi|2128098|pir... 299 4e-80
gb|AAG18904.1| (AE004992) glutamate decarboxylase; GadD [Halobac... 237 1e-61
gi|6677927 sphingosine phosphate lyase 1 [Mus musculus] >gi|7451... 208 9e-53
gb|AAG26964.1|AC069471_25 (AC069471) sphingosine-1-phosphate lya... 202 5e-51
gi|11498921 group II decarboxylase [Archaeoglobus fulgidus] >gi|... 201 9e-51
gi|10190653 sphingosine-1-phosphate lyase 1; Sphingosine-1-phosp... 201 1e-50
gi|11432276 sphingosine-1-phosphate lyase 1 [Homo sapiens] >gi|5... 201 1e-50
dbj|BAA86566.1| (AB033078) KIAA1252 protein [Homo sapiens] 201 1e-50
gb|AAF57903.1| (AE003804) CG8946 gene product [alt 1] [Drosophil... 199 6e-50
gi|6320500 dihydrosphingosine phosphate lyase (also known as sph... 183 2e-45
pir||T33760 sphingosine-1-phosphate lyase (EC 4.-.-.-) Y66H1B.4 ... 180 2e-44
gi|11499876 group II decarboxylase [Archaeoglobus fulgidus] >gi|... 164 2e-39
pir||T29835 sphingosine-1-phosphate lyase (EC 4.-.-.-) B0222.4 [... 155 6e-37
pir||G72753 hypothetical protein APE0020 - Aeropyrum pernix (str... 148 1e-34
pir||G72452 probable glutamate decarboxylase APE2267 - Aeropyrum... 137 2e-31
sp|P54767|DCE_LYCES GLUTAMATE DECARBOXYLASE (GAD) (ERT D1) >gi|1... 122 7e-27
pir||F70975 probable glutamate decarboxylase - Mycobacterium tub... 121 1e-26
dbj|BAA88152.1| (AB025422) glutamic acid decarboxylase [Aspergil... 121 2e-26
gb|AAC24195.1| (AF020425) glutamate decarboxylase isozyme 1 [Nic... 118 1e-25
gb|AAD20099.1| (AC006532) putative glutamate decarboxylase [Arab... 118 1e-25
sp|Q07346|DCE_PETHY GLUTAMATE DECARBOXYLASE (GAD) >gi|1076648|pi... 118 1e-25
dbj|BAB02870.1| (AB026646) glutamate decarboxylase [Arabidopsis ... 117 2e-25
gb|AAC46188.1| (AF005098) glutamate decarboxylase [Lactococcus l... 117 2e-25
pir||T49478 probable glutamate decarboxylase [imported] - Neuros... 116 3e-25
gb|AAB40608.1| (U54774) glutamate decarboxylase [Nicotiana tabacum] 115 9e-25
dbj|BAB10520.1| (AB005238) glutamate decarboxylase 1 (GAD 1) [Ar... 113 4e-24
gb|AAD20093.1| (AC006532) putative glutamate decarboxylase [Arab... 113 5e-24
sp|Q42521|DCE1_ARATH GLUTAMATE DECARBOXYLASE 1 (GAD 1) >gi|49797... 113 5e-24
pir||T01962 glutamate decarboxylase (EC 4.1.1.15) 2, calmodulin-... 113 5e-24
dbj|BAA24585.1| (AB010789) glutamate decarboxylase [Lactococcus ... 112 6e-24
sp|P05034|DCHS_MORMO HISTIDINE DECARBOXYLASE (HDC) >gi|79014|pir... 112 8e-24
pir||T36342 probable glutamate decarboxylase - Streptomyces coel... 112 8e-24
dbj|BAA78331.1| (AB014076) histidine decarboxylase [Brassica napus] 110 2e-23
pir||S75150 glutamate decarboxylase - Synechocystis sp. (strain ... 110 3e-23
gb|AAF60394.1| (AC006701) contains similarity to Pfam family PF0... 109 4e-23
gb|AAF63121.1|AC009526_6 (AC009526) Putative histidine decarbox... 108 9e-23
gb|AAG22562.1|AF309077_2 (AF309077) glutamate decarboxylase [Lis... 108 1e-22
sp|Q42472|DCE2_ARATH GLUTAMATE DECARBOXYLASE 2 (GAD 2) >gi|11849... 106 6e-22
sp|P28578|DCHS_KLEPL HISTIDINE DECARBOXYLASE (HDC) >gi|435602|gb... 105 1e-21
pir||D72734 probable histidine decarboxylase APE0412 - Aeropyrum... 105 1e-21
pir||B40004 histidine decarboxylase (EC 4.1.1.22) - Klebsiella p... 104 2e-21
gb|AAG22560.1|AF309076_1 (AF309076) glutamate decarboxylase [Lis... 102 9e-21
sp|P54772|DCHS_LYCES HISTIDINE DECARBOXYLASE (HDC) (TOM92) >gi|4... 99 6e-20
sp|P28577|DCHS_ENTAE HISTIDINE DECARBOXYLASE (HDC) >gi|95555|pir... 97 4e-19
pir||H82234 probable glutamate decarboxylase VC1149 [imported] -... 92 1e-17
emb|CAA70530.1| (Y09356) pyridoxal-dependent histidine decarboxy... 92 1e-17
sp|P80063|DCEA_ECOLI GLUTAMATE DECARBOXYLASE ALPHA (GAD-ALPHA) >... 91 2e-17
sp|P28302|DCEB_ECOLI GLUTAMATE DECARBOXYLASE BETA (GAD-BETA) >gi... 91 2e-17
sp|Q9Z3R1|RHSB_RHIME L-2,4-DIAMINOBUTYRATE DECARBOXYLASE (DABA D... 90 5e-17
pir||S49218 histidine decarboxylase (EC 4.1.1.22) - Vibrio angui... 88 1e-16
dbj|BAA15157.1| (D90790) Glutamate decarboxylase (EC 4.1.1.15) b... 88 1e-16
gi|6323905 glutamate decarboxylase; Gad1p [Saccharomyces cerevis... 86 6e-16
emb|CAA44834.1| (X63123) glutamate decarboxylase [Escherichia coli] 86 6e-16
pir||T44576 L-2,4-diaminobutyrate decarboxylase (EC 4.1.1.-) [im... 86 6e-16
gb|AAF81243.1| (AF263012) amino acid decarboxylase-like protein ... 84 3e-15
gi|11120696 cysteine-sulfinate decarboxylase [Rattus norvegicus]... 83 4e-15
gb|AAG12476.1|AC037197_1 (AC037197) Putatitve histidine decarbox... 83 4e-15
pir||S55689 sulfoalanine decarboxylase (EC 4.1.1.29) - rat >gi|8... 83 7e-15
dbj|BAB06342.1| (AP001516) L-2,4-diaminobutyrate decarboxylase [... 79 7e-14
emb|CAB71551.1| (AJ270937) aromatic amino acid decarboxylase [So... 79 7e-14
gb|AAF73187.1|AF149833_1 (AF149833) glutamic acid decarboxylase ... 78 1e-13
gb|AAG33932.1|AF045595_1 (AF045595) glutamic acid decarboxylase ... 78 2e-13
gb|AAG20873.1| (AE005155) L-2,4-diaminobutyrate decarboxylase; B... 78 2e-13
sp|Q9Y600|CSD_HUMAN CYSTEINE SULFINIC ACID DECARBOXYLASE (SULFIN... 77 4e-13
gb|AAD32546.1|AF116548_1 (AF116548) cysteine sulfinic acid decar... 76 5e-13
emb|CAB87219.1| (AL163641) putative pyridoxal-dependent decarbox... 73 6e-12
pir||T35762 probable amino acid decarboxylase - Streptomyces coe... 71 2e-11
sp|P71362|DDC_HAEIN L-2,4-DIAMINOBUTYRATE DECARBOXYLASE (DABA DE... 70 5e-11
pir||T26636 hypothetical protein Y37D8A.23 - Caenorhabditis eleg... 69 6e-11
>pir||G75102 group II decarboxylase PAB1578 - Pyrococcus abyssi (strain Orsay)
>gi|5458636|emb|CAB50124.1| (AJ248286) GROUP II
DECARBOXYLASE [Pyrococcus abyssi]
Length = 384
Score = 773 bits (1974), Expect = 0.0
Identities = 384/384 (100%), Positives = 384/384 (100%)
Query: 1 MSKFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDP 60
MSKFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDP
Sbjct: 1 MSKFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDP 60
Query: 61 GLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANILAVRAFRNISDAERPELILPK 120
GLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANILAVRAFRNISDAERPELILPK
Sbjct: 61 GLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANILAVRAFRNISDAERPELILPK 120
Query: 121 SAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIP 180
SAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIP
Sbjct: 121 SAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIP 180
Query: 181 ALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIP 240
ALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIP
Sbjct: 181 ALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIP 240
Query: 241 AGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIV 300
AGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIV
Sbjct: 241 AGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIV 300
Query: 301 RKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKNLRKVERELKRRGWGISAHRGYIR 360
RKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKNLRKVERELKRRGWGISAHRGYIR
Sbjct: 301 RKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKNLRKVERELKRRGWGISAHRGYIR 360
Query: 361 IVFMPHVTKEMVEEFLRDLREVLK 384
IVFMPHVTKEMVEEFLRDLREVLK
Sbjct: 361 IVFMPHVTKEMVEEFLRDLREVLK 384
>pir||D71084 hypothetical protein PH0937 - Pyrococcus horikoshii
>gi|3257351|dbj|BAA30034.1| (AP000004) 383aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 383
Score = 708 bits (1807), Expect = 0.0
Identities = 340/381 (89%), Positives = 366/381 (95%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
KFP GLP+E+V+ L+ +KTK DLTFSSGKILGSMCTMPH+LAIEV+ +YIDRNLGDPGL
Sbjct: 2 KFPRIGLPKEKVIELINEKTKKDLTFSSGKILGSMCTMPHDLAIEVYTKYIDRNLGDPGL 61
Query: 63 HPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANILAVRAFRNISDAERPELILPKSA 122
HPGTRKIEEEVIEM+SDLLHLEKG+GHIVSGGTEANILAVRAFRN+SD E+PELILPKSA
Sbjct: 62 HPGTRKIEEEVIEMISDLLHLEKGHGHIVSGGTEANILAVRAFRNLSDVEKPELILPKSA 121
Query: 123 HFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPAL 182
HFSFIKAGEMLGVKLVWAEL DY VDV+DVEAKISDNTIGIVGIAGTTGLGVVDDIPAL
Sbjct: 122 HFSFIKAGEMLGVKLVWAELNPDYTVDVRDVEAKISDNTIGIVGIAGTTGLGVVDDIPAL 181
Query: 183 SDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAG 242
SDLAR+YGIPLHVDAAFGGFVIPFAK LGY+LPDFDFKLKGV+SITIDPHKMGMAPIPAG
Sbjct: 182 SDLARDYGIPLHVDAAFGGFVIPFAKELGYELPDFDFKLKGVQSITIDPHKMGMAPIPAG 241
Query: 243 GIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRK 302
GI+FRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV+AVWALIKHLGFEGY IV +
Sbjct: 242 GIVFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVIAVWALIKHLGFEGYMRIVER 301
Query: 303 AMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKNLRKVERELKRRGWGISAHRGYIRIV 362
AM+LSRWFAEEIKK+NNAWLVREPMLNIVSFQTKNL+KVERELK RGWGISAHRGYIRIV
Sbjct: 302 AMKLSRWFAEEIKKINNAWLVREPMLNIVSFQTKNLKKVERELKSRGWGISAHRGYIRIV 361
Query: 363 FMPHVTKEMVEEFLRDLREVL 383
FMPHVT+EM+EEFL+DL+EVL
Sbjct: 362 FMPHVTREMIEEFLKDLKEVL 382
>pir||E69015 glutamate decarboxylase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622218|gb|AAB85605.1| (AE000881)
glutamate decarboxylase [Methanobacterium
thermoautotrophicum]
Length = 363
Score = 359 bits (912), Expect = 3e-98
Identities = 182/361 (50%), Positives = 254/361 (69%), Gaps = 7/361 (1%)
Query: 26 LTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEK 85
+T++SG+ILGSMCT H LA V+ +++ NLGDPGL GTR++E VI ML +LL
Sbjct: 1 MTYTSGRILGSMCTSSHPLARRVYCDFLESNLGDPGLFRGTRELESGVIGMLGELLSEPD 60
Query: 86 GYGHIVSGGTEANILAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQD 145
GHI++GGTEAN++A+RA RN++ AE+PE+I+PKSAHFSF KA ++LG++L AEL QD
Sbjct: 61 AAGHIITGGTEANLMAMRAARNMAGAEKPEIIVPKSAHFSFRKAADILGLRLREAELDQD 120
Query: 146 YAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIP 205
Y VDV+ V IS+NT+ +VG+AGTT LG +D + LS++ + I LH+DAAFGGF+IP
Sbjct: 121 YRVDVESVRKLISENTVAVVGVAGTTELGRIDPVEELSEICLDEDIHLHIDAAFGGFIIP 180
Query: 206 FAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGG 265
F + G +LP+FDFKL+GV SIT+DPHKMG+APIP+G I+FR YL A+S+ PYL
Sbjct: 181 FLRETGAELPEFDFKLQGVSSITVDPHKMGLAPIPSGCILFRDASYLDAMSIETPYLT-- 238
Query: 266 KVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVRE 325
+ Q+TI GTR GAS A WA++KH+G EGYR++ + M ++R + + +L+ LV E
Sbjct: 239 EKQQSTIVGTRTGASAAATWAIMKHMGREGYRKLALRVMGVTRRLRDGLVELDYQ-LVVE 297
Query: 326 PMLNIVSFQTKNL--RKVERELKRRGWGISAHR--GYIRIVFMPHVTKEMVEEFLRDLRE 381
P LNIV+F + ++ L+ GW +S IR+V MPH+ +E +E LRDL
Sbjct: 298 PELNIVAFNHPAMGPHELADRLEELGWAVSVSSCPPAIRVVLMPHIMEEHIELLLRDLEG 357
Query: 382 V 382
+
Sbjct: 358 I 358
>gi|11499586 group II decarboxylase [Archaeoglobus fulgidus]
>gi|7436490|pir||C69500 group II decarboxylase homolog -
Archaeoglobus fulgidus >gi|2648533|gb|AAB89250.1|
(AE000964) group II decarboxylase [Archaeoglobus
fulgidus]
Length = 367
Score = 344 bits (873), Expect = 1e-93
Identities = 180/375 (48%), Positives = 253/375 (67%), Gaps = 13/375 (3%)
Query: 13 EVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGLHPGTRKIEEE 72
+++ L + D+ +S ++L SMCT+PH +A+E +I+ NLGDPG+ GT ++E +
Sbjct: 2 DIIEELRAYREKDIPYS--RVLSSMCTVPHPVAVEAHRMFIETNLGDPGIFRGTVELEAK 59
Query: 73 VIEMLSDLLHLEKGYGHIVSGGTEANILAVRAFRNISDAERPELILPKSAHFSFIKAGEM 132
++ ++ D+LH E G+I SGGTEANI +RA RN+ E P +++PK+AHFSF K G++
Sbjct: 60 LMRLIGDILHCETPAGYICSGGTEANIQGIRAARNVQKKENPNIVIPKTAHFSFEKIGDI 119
Query: 133 LGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIP 192
LGVK+ A + ++Y VDV VE + +NT+ IVGIAGTT LG +D I LS LA E +
Sbjct: 120 LGVKIKRAGVDEEYKVDVGQVEDLMDENTVAIVGIAGTTELGQIDPIVELSKLAEERQVE 179
Query: 193 LHVDAAFGGFVIPFAKSLGYDLP-DFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKY 251
LHVDAAFGG VIPF D P FDF+ +GV SITIDPHKMGMA IPAGGIIFR + Y
Sbjct: 180 LHVDAAFGGLVIPF-----MDNPYPFDFQNRGVSSITIDPHKMGMATIPAGGIIFRNESY 234
Query: 252 LKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFA 311
L+A+ V PYL Q T+TGTRPG V + +A++K LGFEG RE+V+ ++ +R
Sbjct: 235 LRALEVETPYLTSKT--QFTLTGTRPGTGVASAYAVLKSLGFEGMREVVKNCLKNTRILV 292
Query: 312 EEIKKLNNAWLVREPMLNIVSFQTKNLRKVERELKRRGWGISAHR--GYIRIVFMPHVTK 369
EE++ L ++ EP++N+VSF+T +++ EL R W IS R IR V MPHVT+
Sbjct: 293 EEMRDLGFEPVI-EPVMNVVSFRTDEAERIKEELYRMRWVISTIREPKAIRFVVMPHVTE 351
Query: 370 EMVEEFLRDLREVLK 384
E+++ F+ D R+VL+
Sbjct: 352 EVIKNFISDFRKVLR 366
>sp|Q60358|Y050_METJA HYPOTHETICAL PROTEIN MJ0050 >gi|2128098|pir||B64306 hypothetical
protein MJ0050 - Methanococcus jannaschii
>gi|1498811|gb|AAB98031.1| (U67463) group II
decarboxylase [Methanococcus jannaschii]
Length = 396
Score = 299 bits (757), Expect = 4e-80
Identities = 168/400 (42%), Positives = 252/400 (63%), Gaps = 25/400 (6%)
Query: 1 MSKFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDP 60
M EKG+ +E+L L+ +DL + G I GSMC+ + ++ +++ NLGDP
Sbjct: 1 MRNMQEKGVSEKEILEELKKYRSLDLKYEDGNIFGSMCSNVLPITRKIVDIFLETNLGDP 60
Query: 61 GLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANILAVRAFRNI---------SDA 111
GL GT+ +EE+ + +L LL+ + YGHIVSGGTEAN++A+R +NI S
Sbjct: 61 GLFKGTKLLEEKAVALLGSLLNNKDAYGHIVSGGTEANLMALRCIKNIWREKRRKGLSKN 120
Query: 112 ERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTI-GIVGIAGT 170
E P++I+P +AHFSF K EM+ ++ ++A +K+DY +D K V+ + D + GI+GIAGT
Sbjct: 121 EHPKIIVPITAHFSFEKGREMMDLEYIYAPIKEDYTIDEKFVKDAVEDYDVDGIIGIAGT 180
Query: 171 TGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPF------AKSLGYDLPDFDFKLKGV 224
T LG +D+I LS +A+E I +HVDAAFGG VIPF K + Y FDF L GV
Sbjct: 181 TELGTIDNIEELSKIAKENNIYIHVDAAFGGLVIPFLDDKYKKKGVNY---KFDFSL-GV 236
Query: 225 ESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAV 284
+SITIDPHKMG PIP+GGI+F+ Y + + V APYL + QATI GTR G
Sbjct: 237 DSITIDPHKMGHCPIPSGGILFKDIGYKRYLDVDAPYLT--ETRQATILGTRVGFGGACT 294
Query: 285 WALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKNLRKVERE 344
+A++++LG EG R+IV + ME + + +++K+ NN V EP+LNIV+ + ++ ++V ++
Sbjct: 295 YAVLRYLGREGQRKIVNECMENTLYLYKKLKE-NNFKPVIEPILNIVAIEDEDYKEVCKK 353
Query: 345 LKRRGWGISAHR--GYIRIVFMPHVTKEMVEEFLRDLREV 382
L+ RG +S +RIV MPH+ +E ++ F+ L +
Sbjct: 354 LRDRGIYVSVCNCVKALRIVVMPHIKREHIDNFIEILNSI 393
>gb|AAG18904.1| (AE004992) glutamate decarboxylase; GadD [Halobacterium sp. NRC-1]
Length = 355
Score = 237 bits (599), Expect = 1e-61
Identities = 138/349 (39%), Positives = 199/349 (56%), Gaps = 13/349 (3%)
Query: 32 KILGSMCTMPHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGYGHIV 91
++L SMCT PH A E ++ N GDP +P + E + + +L +++ L +G+I
Sbjct: 15 RVLSSMCTTPHPAAREAAQAFLATNPGDPETYPAVAERERDAVALLGEIVGLSSPHGYIA 74
Query: 92 SGGTEANILAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVK 151
+GGTEAN+ AVRA RN +DA+ ++ P SAHFSF KA ++LGV+L A D+ DV
Sbjct: 75 AGGTEANLQAVRAARNRADADAVNVVAPASAHFSFQKAADVLGVELRLAPTDGDHRADVA 134
Query: 152 DVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLG 211
V + +T +VG+AGTT G VD IPAL+D+A LHVDAA+GGFV+PF
Sbjct: 135 AVADLVDGDTAVVVGVAGTTEYGRVDPIPALADIAAGVDANLHVDAAWGGFVLPFTDH-- 192
Query: 212 YDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQAT 271
D+ F V ++ IDPHKMG AP+PAGG + R + L A+++ PYL Q T
Sbjct: 193 ----DWSFADAPVNTMAIDPHKMGQAPVPAGGFLARDPETLDALAIETPYLESDT--QPT 246
Query: 272 ITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIV 331
+ GTR GA V A ++ L +GYRE + + + A E+ + V +P L +V
Sbjct: 247 LGGTRSGAGVAGALASLRALWPDGYREQYERTQGNAEYLAAELAA--RGYDVVDPELPLV 304
Query: 332 SFQTKNLRKVERELKRRGWGIS-AHRGYIRIVFMPHVTKEMVEEFLRDL 379
+ + + L+ GW IS +R+V MPHVT+EM+ FL D+
Sbjct: 305 AADMPDAE--FQALREEGWRISRTASDALRVVCMPHVTREMLAAFLDDV 351
>gi|6677927 sphingosine phosphate lyase 1 [Mus musculus]
>gi|7451520|pir||JC5923 sphingosine-1-phosphate lyase
(EC 4.-.-.-) [validated] - mouse
>gi|2906011|gb|AAC03768.1| (AF036894)
sphingosine-1-phosphate lyase; pyridoxal-phosphate
protein; SPL [Mus musculus]
Length = 568
Score = 208 bits (524), Expect = 9e-53
Identities = 120/390 (30%), Positives = 210/390 (53%), Gaps = 13/390 (3%)
Query: 5 PEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPH---ELAIEVFARYIDRNLGDPG 61
P +G+ EVL L++ + +D ++ GK G++ EL ++ + + N P
Sbjct: 117 PAQGMGTAEVLERLKEYSSMDGSWQEGKASGAVYNGEPKLTELLVQAYGEFTWSNPLHPD 176
Query: 62 LHPGTRKIEEEVIEMLSDLLHL-EKGYGHIVSGGTEANILAVRAFRNIS---DAERPELI 117
+ PG RK+E E++ M L + G + SGGTE+ ++A +A+R+++ + PE++
Sbjct: 177 IFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGGTESILMACKAYRDLALEKGIKTPEIV 236
Query: 118 LPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVD 177
P+SAH +F KA G+K+V LK++ VDV+ ++ IS NT +V GV+D
Sbjct: 237 APESAHAAFDKAAHYFGMKIVRVALKKNMEVDVQAMKRAISRNTAMLVCSTPQFPHGVMD 296
Query: 178 DIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDL-PDFDFKLKGVESITIDPHKMGM 236
+P ++ L Y IPLHVDA GGF+I F + GY L FDF++KGV SI+ D HK G
Sbjct: 297 PVPEVAKLTVRYKIPLHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGY 356
Query: 237 APIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGY 296
AP + +++ +KY + GG +I G+RPG + A WA + H G GY
Sbjct: 357 APKGSSVVMYSNEKYRTYQFFVGADWQGGVYASPSIAGSRPGGIIAACWAALMHFGENGY 416
Query: 297 REIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRGWG--- 351
E ++ ++ +R+ E++ + N ++ +P L++++ + ++ ++ + +GW
Sbjct: 417 VEATKQIIKTARFLKSELENIKNIFIFGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNY 476
Query: 352 ISAHRGYIRIVFMPHVTKEMVEEFLRDLRE 381
+ R + + H K + +FL+D+RE
Sbjct: 477 LQFPRSIHFCITLVHTRKRVAIQFLKDIRE 506
>gb|AAG26964.1|AC069471_25 (AC069471) sphingosine-1-phosphate lyase, putative [Arabidopsis
thaliana]
Length = 544
Score = 202 bits (509), Expect = 5e-51
Identities = 119/332 (35%), Positives = 190/332 (56%), Gaps = 17/332 (5%)
Query: 68 KIEEEVIEMLSDLLHLEKGY------GHIVSGGTEANILAVRAFRNISDAE----RPELI 117
+ E EV+ M + LL ++ G++ SGGTE+ +LAV++ R+ + RPE+I
Sbjct: 174 RFESEVVAMTAALLGSKETASGGQICGNMTSGGTESIVLAVKSSRDYMKYKKGITRPEMI 233
Query: 118 LPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVD 177
+P+S H ++ KA + +KL + +D+ DVK I+ NTI IVG A G++D
Sbjct: 234 IPESGHSAYDKAAQYFKIKLWRVPVDKDFRADVKATRRHINRNTIMIVGSAPGFPHGIID 293
Query: 178 DIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMA 237
I L LA YGI HVD GGFV+PFA+ LGY +P FDF ++GV SI++D HK G+A
Sbjct: 294 PIEELGQLALSYGICFHVDLCLGGFVLPFARKLGYQIPPFDFSVQGVTSISVDVHKYGLA 353
Query: 238 PIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYR 297
P +++R + K V +GG TI G+RPG+ V WA + LG EGY
Sbjct: 354 PKGTSTVLYRNHEIRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYL 413
Query: 298 EIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRGWGISAH 355
+ K ME S+ E +++++ +++ +P + IV+F +K ++ +V + +GW ++A
Sbjct: 414 QNTSKIMEASKRLEEGVREIHELFVIGKPDMTIVAFGSKALDIFEVNDIMSSKGWHLNAL 473
Query: 356 RGYIRI---VFMPHVTKEMVEEFLRDLREVLK 384
+ I + + HV +V++FLRDLRE ++
Sbjct: 474 QRPNSIHICITLQHV--PVVDDFLRDLREAVE 503
>gi|11498921 group II decarboxylase [Archaeoglobus fulgidus]
>gi|7436489|pir||B69415 group II decarboxylase homolog -
Archaeoglobus fulgidus >gi|2649253|gb|AAB89922.1|
(AE001012) group II decarboxylase [Archaeoglobus
fulgidus]
Length = 488
Score = 201 bits (507), Expect = 9e-51
Identities = 135/399 (33%), Positives = 213/399 (52%), Gaps = 21/399 (5%)
Query: 4 FPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPH----ELAIEVFARYIDRNLGD 59
FP G E VL LED K D S ++ G + ELA + + Y+D+ + D
Sbjct: 34 FPN-GSDAEGVLKRLEDYAKNDFEPHSRRMWGHIYYAGLKDVVELARKAYLMYMDKTMLD 92
Query: 60 PGLHPGTRKIEEEVIEMLSDLLHL-EKGYGHIVSGGTEANILAVRAFRNISDAER----- 113
P ++E EV+ M S LL+ E+ G+ GGTE+ +LA++A R E
Sbjct: 93 FTCFPSLLRMEREVVRMASSLLNGDEEVVGNFTYGGTESIMLALKAAREKFRKEEGGNVV 152
Query: 114 PELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGL 173
PE++LP +AH +F K+ E LG++ + A+L + DV+ V+ + D T IVG A
Sbjct: 153 PEIVLPATAHPAFWKSAEYLGMRCLRAKLDDELRADVETVKELVGDKTAMIVGSAPNYPF 212
Query: 174 GVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHK 233
GVVDDI ALSD+A + + LHVDA GGF +PF + LG +PDFDF ++GV SI+ D HK
Sbjct: 213 GVVDDIKALSDIAVDGKLWLHVDACLGGFHLPFFRELGEKIPDFDFSVEGVHSISADFHK 272
Query: 234 MGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGF 293
G++P A I++R K + + G + + TR ++ A WA++ +LGF
Sbjct: 273 YGLSPRGASVILYRNAKLREGQIFVMASWPGYPLVNTAVLSTRSAGTLAAAWAVMSYLGF 332
Query: 294 EGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRGWG 351
+GY ++ +K + + + + +L L+ P +++F ++ NL KV + +GW
Sbjct: 333 DGYLKLAKKTLYAKKRLIDGLTEL-GLELLGSPEGAVLAFTSERHNLFKVSTLMAEKGWY 391
Query: 352 ISAHRGYIRIVFMPHV-------TKEMVEEFLRDLREVL 383
+ + G ++ F + E+V+EFL D+REVL
Sbjct: 392 VQSQPGSKKLGFPRSLHFSVIPGHAEVVDEFLEDMREVL 430
>gi|10190653 sphingosine-1-phosphate lyase 1; Sphingosine-1-phosphate lyase
[Homo sapiens] >gi|10129683|emb|CAA09590.2| (AJ011304)
sphingosine-1-phosphate lyase [Homo sapiens]
Length = 568
Score = 201 bits (506), Expect = 1e-50
Identities = 121/390 (31%), Positives = 208/390 (53%), Gaps = 13/390 (3%)
Query: 5 PEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPH---ELAIEVFARYIDRNLGDPG 61
P +GL VL L++ + +D + G+ G++ + EL ++ + + N P
Sbjct: 117 PSQGLSSSAVLEKLKEYSSMDAFWQEGRASGTVYSGEEKLTELLVKAYGDFAWSNPLHPD 176
Query: 62 LHPGTRKIEEEVIEMLSDLLHL-EKGYGHIVSGGTEANILAVRAFRNIS---DAERPELI 117
+ PG RKIE E++ + L + G + SGGTE+ ++A +A+R+++ + PE++
Sbjct: 177 IFPGLRKIEAEIVRIACSLFNGGPDSCGCVTSGGTESILMACKAYRDLAFEKGIKTPEIV 236
Query: 118 LPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVD 177
P+SAH +F KA G+K+V L + VDV+ + IS NT +V GV+D
Sbjct: 237 APQSAHAAFNKAASYFGMKIVRVPLTKMMEVDVRAMRRAISRNTAMLVCSTPQFPHGVID 296
Query: 178 DIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLP-DFDFKLKGVESITIDPHKMGM 236
+P ++ LA +Y IPLHVDA GGF+I F + GY L FDF++KGV SI+ D HK G
Sbjct: 297 PVPEVAKLAVKYKIPLHVDACLGGFLIVFMEKAGYPLEHPFDFRVKGVTSISADTHKYGY 356
Query: 237 APIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGY 296
AP + +++ KKY + GG TI G+RPG A WA + H G GY
Sbjct: 357 APKGSSLVLYSDKKYRNYQFFVDTDWQGGIYASPTIAGSRPGGISAAAWAALMHFGENGY 416
Query: 297 REIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRGWGISA 354
E ++ ++ +R+ E++ + ++ P L++++ ++ ++ ++ + +GW ++
Sbjct: 417 VEATKQIIKTARFLKSELENIKGIFVFGNPQLSVIALGSRDFDIYRLSNLMTAKGWNLNQ 476
Query: 355 HRGYIRIVF---MPHVTKEMVEEFLRDLRE 381
+ I F + H K + +FL+D+RE
Sbjct: 477 LQFPPSIHFCITLLHARKRVAIQFLKDIRE 506
>gi|11432276 sphingosine-1-phosphate lyase 1 [Homo sapiens]
>gi|5532487|gb|AAD44755.1|AF144638_1 (AF144638)
sphingosine-1-phosphate lyase [Homo sapiens]
Length = 568
Score = 201 bits (505), Expect = 1e-50
Identities = 121/390 (31%), Positives = 208/390 (53%), Gaps = 13/390 (3%)
Query: 5 PEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPH---ELAIEVFARYIDRNLGDPG 61
P +GL VL L++ + +D + G+ G++ + EL ++ + + N P
Sbjct: 117 PSQGLSSSAVLEKLKEYSSMDAFWQEGRASGTVYSGEEKLTELLVKAYGDFAWSNPLHPD 176
Query: 62 LHPGTRKIEEEVIEMLSDLLHL-EKGYGHIVSGGTEANILAVRAFRNIS---DAERPELI 117
+ PG RKIE E++ + L + G + SGGTE+ ++A +A+R+++ + PE++
Sbjct: 177 IFPGLRKIEAEIVRIACSLFNGGPDSCGCVTSGGTESILMACKAYRDLAFEKGIKTPEIV 236
Query: 118 LPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVD 177
P+SAH +F KA G+K+V L + VDV+ + IS NT +V GV+D
Sbjct: 237 APQSAHAAFNKAASYFGMKIVRVPLTKMMEVDVRAMRRAISRNTAMLVCSTPQFPHGVID 296
Query: 178 DIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLP-DFDFKLKGVESITIDPHKMGM 236
+P ++ LA +Y IPLHVDA GGF+I F + GY L FDF++KGV SI+ D HK G
Sbjct: 297 PVPEVAKLAVKYKIPLHVDACLGGFLIVFMEKAGYPLEHPFDFRVKGVTSISADTHKYGY 356
Query: 237 APIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGY 296
AP + +++ KKY + GG TI G+RPG A WA + H G GY
Sbjct: 357 APKGSSLVLYSDKKYRNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWAALMHFGENGY 416
Query: 297 REIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRGWGISA 354
E ++ ++ +R+ E++ + ++ P L++++ ++ ++ ++ + +GW ++
Sbjct: 417 VEATKQIIKTARFLKSELENIKGIFVFGNPQLSVIALGSRDFDIYRLSNLMTAKGWNLNQ 476
Query: 355 HRGYIRIVF---MPHVTKEMVEEFLRDLRE 381
+ I F + H K + +FL+D+RE
Sbjct: 477 LQFPPSIHFCITLLHARKRVAIQFLKDIRE 506
>dbj|BAA86566.1| (AB033078) KIAA1252 protein [Homo sapiens]
Length = 580
Score = 201 bits (505), Expect = 1e-50
Identities = 121/390 (31%), Positives = 208/390 (53%), Gaps = 13/390 (3%)
Query: 5 PEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPH---ELAIEVFARYIDRNLGDPG 61
P +GL VL L++ + +D + G+ G++ + EL ++ + + N P
Sbjct: 129 PSQGLSSSAVLEKLKEYSSMDAFWQEGRASGTVYSGEEKLTELLVKAYGDFAWSNPLHPD 188
Query: 62 LHPGTRKIEEEVIEMLSDLLHL-EKGYGHIVSGGTEANILAVRAFRNIS---DAERPELI 117
+ PG RKIE E++ + L + G + SGGTE+ ++A +A+R+++ + PE++
Sbjct: 189 IFPGLRKIEAEIVRIACSLFNGGPDSCGCVTSGGTESILMACKAYRDLAFEKGIKTPEIV 248
Query: 118 LPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVD 177
P+SAH +F KA G+K+V L + VDV+ + IS NT +V GV+D
Sbjct: 249 APQSAHAAFNKAASYFGMKIVRVPLTKMMEVDVRAMRRAISRNTAMLVCSTPQFPHGVID 308
Query: 178 DIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLP-DFDFKLKGVESITIDPHKMGM 236
+P ++ LA +Y IPLHVDA GGF+I F + GY L FDF++KGV SI+ D HK G
Sbjct: 309 PVPEVAKLAVKYKIPLHVDACLGGFLIVFMEKAGYPLEHPFDFRVKGVTSISADTHKYGY 368
Query: 237 APIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGY 296
AP + +++ KKY + GG TI G+RPG A WA + H G GY
Sbjct: 369 APKGSSLVLYSDKKYRNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWAALMHFGENGY 428
Query: 297 REIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRGWGISA 354
E ++ ++ +R+ E++ + ++ P L++++ ++ ++ ++ + +GW ++
Sbjct: 429 VEATKQIIKTARFLKSELENIKGIFVFGNPQLSVIALGSRDFDIYRLSNLMTAKGWNLNQ 488
Query: 355 HRGYIRIVF---MPHVTKEMVEEFLRDLRE 381
+ I F + H K + +FL+D+RE
Sbjct: 489 LQFPPSIHFCITLLHARKRVAIQFLKDIRE 518
>gb|AAF57903.1| (AE003804) CG8946 gene product [alt 1] [Drosophila melanogaster]
>gi|7302830|gb|AAF57904.1| (AE003804) CG8946 gene
product [alt 2] [Drosophila melanogaster]
>gi|10636392|emb|CAC10531.1| (AJ297394)
sphingosine-phosphate lyase [Drosophila melanogaster]
Length = 545
Score = 199 bits (500), Expect = 6e-50
Identities = 116/391 (29%), Positives = 208/391 (52%), Gaps = 15/391 (3%)
Query: 5 PEKGLPREEVLNLLEDKTKVD-LTFSSGKILGSMCTMPH---ELAIEVFARYIDRNLGDP 60
PEKGL +EE+L L+++ K + G++ G++ EL EV+ + N
Sbjct: 104 PEKGLSKEEILRLVDEHLKTGHYNWRDGRVSGAVYGYKPDLVELVTEVYGKASYTNPLHA 163
Query: 61 GLHPGTRKIEEEVIEMLSDLLHLEKGY-GHIVSGGTEANILAVRAFRNIS----DAERPE 115
L PG K+E EV+ M +L H G + +GGTE+ ++A++A+R+ + RP
Sbjct: 164 DLFPGVCKMEAEVVRMACNLFHGNSASCGTMTTGGTESIVMAMKAYRDFAREYKGITRPN 223
Query: 116 LILPKSAHFSFIKAGEMLGVKLVWAELKQD-YAVDVKDVEAKISDNTIGIVGIAGTTGLG 174
+++PK+ H +F K G+ + + ++ + Y VD+K + I+ NTI +VG A G
Sbjct: 224 IVVPKTVHAAFDKGGQYFNIHVRSVDVDPETYEVDIKKFKRAINRNTILLVGSAPNFPYG 283
Query: 175 VVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKM 234
+DDI A++ L +Y IP+HVDA G FV+ ++ GY L FDF++KGV SI+ D HK
Sbjct: 284 TIDDIEAIAALGVKYDIPVHVDACLGSFVVALVRNAGYKLRPFDFEVKGVTSISADTHKY 343
Query: 235 GMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFE 294
G AP + I++ KKY + GG T+ G+R G + A WA + G++
Sbjct: 344 GFAPKGSSVILYSDKKYKDHQFTVTTDWPGGVYGSPTVNGSRAGGIIAACWATMMSFGYD 403
Query: 295 GYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRGWGI 352
GY E ++ ++ +R+ ++ ++ ++ +P ++++ + ++ ++ L + GW +
Sbjct: 404 GYLEATKRIVDTARYIERGVRDIDGIFIFGKPATSVIALGSNVFDIFRLSDSLCKLGWNL 463
Query: 353 SAHR---GYIRIVFMPHVTKEMVEEFLRDLR 380
+A + G V H + ++F+ D+R
Sbjct: 464 NALQFPSGIHLCVTDMHTQPGVADKFIADVR 494
>gi|6320500 dihydrosphingosine phosphate lyase (also known as sphingosine
phosphate lyase); Dpl1p [Saccharomyces cerevisiae]
>gi|2132493|pir||S70123 sphingosine-1-phosphate lyase
(EC 4.-.-.-) [similarity] - yeast (Saccharomyces
cerevisiae) >gi|1230658|gb|AAB64470.1| (U51031) Ydr294cp
[Saccharomyces cerevisiae]
Length = 589
Score = 183 bits (461), Expect = 2e-45
Identities = 129/407 (31%), Positives = 206/407 (49%), Gaps = 33/407 (8%)
Query: 3 KFPEKGLPREEV---LNLLEDKTKVDLTFSSGKILGSMCTMPHELA---IEVFARYIDRN 56
+ P G+P+++V LN L D + GK+ G++ +L + +Y N
Sbjct: 137 QLPSNGIPQDDVIEELNKLNDLIP-HTQWKEGKVSGAVYHGGDDLIHLQTIAYEKYCVAN 195
Query: 57 LGDPGLHPGTRKIEEEVIEMLSDLLHL--EKGYGHIVSGGTEANILAVRAFRNISDAER- 113
P + P RK+E EV+ M+ + + + G G SGGTE+ +LA + + + R
Sbjct: 196 QLHPDVFPAVRKMESEVVSMVLRMFNAPSDTGCGTTTSGGTESLLLACLSAKMYALHHRG 255
Query: 114 ---PELILPKSAHFSFIKAGEMLGVKLVWAELKQD-YAVDVKDVEAKISDNTIGIVGIAG 169
PE+I P +AH F KA G+KL EL Y VD+ V+ I+ NTI +VG A
Sbjct: 256 ITEPEIIAPVTAHAGFDKAAYYFGMKLRHVELDPTTYQVDLGKVKKFINKNTILLVGSAP 315
Query: 170 TTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGY-DLPDFDFKLKGVESIT 228
G+ DDI L +A++Y +PLHVD+ G F++ F + GY +LP DF++ GV SI+
Sbjct: 316 NFPHGIADDIEGLGKIAQKYKLPLHVDSCLGSFIVSFMEKAGYKNLPLLDFRVPGVTSIS 375
Query: 229 IDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALI 288
D HK G AP + I++R + P GG T+ G+RPGA V+ WA +
Sbjct: 376 CDTHKYGFAPKGSSVIMYRNSDLRMHQYYVNPAWTGGLYGSPTLAGSRPGAIVVGCWATM 435
Query: 289 KHLGFEGY----REIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVE 342
++G GY +EIV AM+ ++ E I LN ++ P +++SF +K N+ ++
Sbjct: 436 VNMGENGYIESCQEIVGAAMKFKKYIQENIPDLN---IMGNPRYSVISFSSKTLNIHELS 492
Query: 343 RELKRRGWGISA-------HRGYIRIVFMPHVTKEMVEEFLRDLREV 382
L ++GW +A H + R+ HV E+ + ++E+
Sbjct: 493 DRLSKKGWHFNALQKPVALHMAFTRL--SAHVVDEICDILRTTVQEL 537
>pir||T33760 sphingosine-1-phosphate lyase (EC 4.-.-.-) Y66H1B.4 [similarity] -
Caenorhabditis elegans >gi|3801005|gb|AAC69001.1|
(AF100673) similar to pyridoxal-dependent decarboxylases
conserved domain (Pfam: pyridoxal_deC.hmm. score: 76.39)
[Caenorhabditis elegans]
>gi|5532489|gb|AAD44756.1|AF144639_1 (AF144639)
sphingosine-1-phosphate lyase [Caenorhabditis elegans]
Length = 552
Score = 180 bits (452), Expect = 2e-44
Identities = 117/396 (29%), Positives = 202/396 (50%), Gaps = 14/396 (3%)
Query: 1 MSKFPEKGLPREEVLNLLEDKTKVD-LTFSSGKILGSMCTMPH----ELAIEVFARYIDR 55
+S P L ++ ++ L + + G++ G++ T H L +++ +Y
Sbjct: 106 ISTLPIAPLSQDSIMELAKKYEDYNTFNIDGGRVSGAVYTDRHAEHINLLGKIYEKYAFS 165
Query: 56 NLGDPGLHPGTRKIEEEVIEMLSDLLH-LEKGYGHIVSGGTEANILAVRAFRNISDA--- 111
N P + PG RK+E E+I M+ +L + E G + SGGTE+ I+A ++RN + +
Sbjct: 166 NPLHPDVFPGARKMEAELIRMVLNLYNGPEDSSGSVTSGGTESIIMACFSYRNRAHSLGI 225
Query: 112 ERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTT 171
E P ++ K+AH +F KA + G++L + D VD+K++E I N +VG A
Sbjct: 226 EHPVILACKTAHAAFDKAAHLCGMRLRHVPVDSDNRVDLKEMERLIDSNVCMLVGSAPNF 285
Query: 172 GLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDP 231
G +D IP ++ L ++YGIP+HVDA GGF+IPF GY +P FDF+ GV SI+ D
Sbjct: 286 PSGTIDPIPEIAKLGKKYGIPVHVDACLGGFMIPFMNDAGYLIPVFDFRNPGVTSISCDT 345
Query: 232 HKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHL 291
HK G P + +++R K+ GG TI G+R GA+ WA +
Sbjct: 346 HKYGCTPKGSSIVMYRSKELHHFQYFSVADWCGGIYATPTIAGSRAGANTAVAWATLLSF 405
Query: 292 GFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERELKRRG 349
G + Y + ++ +R AE+I+K+ + +++V+F N+ +V ++ + G
Sbjct: 406 GRDEYVRRCAQIVKHTRMLAEKIEKIKWIKPYGKSDVSLVAFSGNGVNIYEVSDKMMKLG 465
Query: 350 WGISAHRGYIRI---VFMPHVTKEMVEEFLRDLREV 382
W ++ + I + + +E+V F DL ++
Sbjct: 466 WNLNTLQNPAAIHICLTINQANEEVVNAFAVDLEKI 501
>gi|11499876 group II decarboxylase [Archaeoglobus fulgidus]
>gi|7436488|pir||G69536 group II decarboxylase homolog -
Archaeoglobus fulgidus >gi|2648227|gb|AAB88962.1|
(AE000946) group II decarboxylase [Archaeoglobus
fulgidus]
Length = 414
Score = 164 bits (411), Expect = 2e-39
Identities = 118/393 (30%), Positives = 196/393 (49%), Gaps = 24/393 (6%)
Query: 12 EEVLNLLEDKTKVDLTFSSGKILGSMCTMPHE----LAIEVFARYIDRNLGDPGLHPGTR 67
++VL+LLE DL +G++ + E +A + R+ ++NL D +
Sbjct: 4 DDVLSLLEKAESEDLNPRTGRLFAYVYETGDENIRKVAEKALVRFAEKNLLDFTVFRSAV 63
Query: 68 KIEEEVIEMLSDLLHLEKGYGHIVSGGTEANILAVRAFRNI-----SDAERPELILPKSA 122
E+EV+ +L+H + G GGTE+ +LAV+A R+ AE PE++ P S
Sbjct: 64 FFEKEVVGFARNLMHGDAAVGSFTFGGTESIMLAVKAARDYYRKKEGTAEVPEILAPISI 123
Query: 123 HFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPAL 182
H +F+KA + LG+K+V +K D DV +S T I A G +D + +
Sbjct: 124 HPAFLKAADYLGLKVVRLPVK-DAKGDVDAFAEAVSGKTALIALSAPNWPFGTIDPVEEI 182
Query: 183 SDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAG 242
+++A E + LHVDA GGF++PF + LG +P FDF+++GV SI++D HK G AP A
Sbjct: 183 AEIAAERNVLLHVDACLGGFILPFFEMLGEKIPKFDFRVEGVTSISLDAHKYGYAPKGAS 242
Query: 243 GIIFRRKKYLKAISVLAPYLAGGKVW-QATITGTRPGASVLAVWALIKHLGFEGYREIVR 301
++FR + LK S+ + G V+ + +RP + A +A+IK+LG EGY+E+
Sbjct: 243 VVLFRDAE-LKKCSMFVDVTSPGYVFVNQAVLSSRPEGPLAAAFAVIKYLGVEGYKELAS 301
Query: 302 KAMELSRWFAEEIKKL--NNAWLVREPMLNIVSFQTKNLRKVERELKRRGWGISAHRGY- 358
K + +K L + V +L + + +L +K+ GW + +G
Sbjct: 302 KILSARDKIYRGLKSLGFESVGEVESSVLAMTN-PDVDLMGFVNNMKKLGWQLHLQKGLK 360
Query: 359 -------IRIVFMPHVTKEMVEEFLRDLREVLK 384
I + P V + E+F+ D + L+
Sbjct: 361 EYDIPDNIHLTLSP-VHDGVAEKFVEDAAKALE 392
>pir||T29835 sphingosine-1-phosphate lyase (EC 4.-.-.-) B0222.4 [similarity] -
Caenorhabditis elegans >gi|1226312|gb|AAA92321.1|
(U50312) Similar to glutamate decarboxylase
[Caenorhabditis elegans]
Length = 542
Score = 155 bits (389), Expect = 6e-37
Identities = 119/397 (29%), Positives = 194/397 (47%), Gaps = 20/397 (5%)
Query: 5 PEKGLPREEVLNLLEDKTKVD-LTFSSGKILGSMCTMP-----HELAIEVFARYIDRNLG 58
P + R EVL L ++ F G++ G++ E+ EVF ++ N
Sbjct: 102 PSHSVGRTEVLRLAAIYDDLEGPAFLEGRVSGAVFNREDDKDEREMYEEVFGKFAWTNPL 161
Query: 59 DPGLHPGTRKIEEEVIEMLSDLLHLE-KGYGHIVSGGTEANILAVRAFRN--ISDAER-P 114
P L PG R +E EV+ M ++++ + + G + +GG+ + +LA A RN + E+
Sbjct: 162 WPKLFPGVRIMEAEVVRMCCNMMNGDSETCGTMSTGGSISILLACLAHRNRLLKRGEKYT 221
Query: 115 ELILPKSAHFSFIKAGEMLGVKLVWAELKQ-DYAVDVKDVEAKISDNTIGIVGIAGTTGL 173
E+I+P S H +F KA E +K+ + + VD+ ++A I+ T +VG A
Sbjct: 222 EMIVPSSVHAAFFKAAECFRIKVRKIPVDPVTFKVDLVKMKAAINKRTCMLVGSAPNFPF 281
Query: 174 GVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHK 233
G VDDI A+ L EY IP+HVDA GGF++PF L D +DF++ GV SI+ D HK
Sbjct: 282 GTVDDIEAIGQLGLEYDIPVHVDACLGGFLLPF---LEEDEIRYDFRVPGVSSISADSHK 338
Query: 234 MGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGF 293
G+AP + +++R K+ L GG AT+ G+R G ++ WA + +
Sbjct: 339 YGLAPKGSSVVLYRNKELLHNQYFCDADWQGGIYASATMEGSRAGHNIALCWAAMLYHAQ 398
Query: 294 EGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKN---LRKVERELKRRGW 350
EGY+ RK ++ +R + + L + IVS+ T + L + +K + W
Sbjct: 399 EGYKANARKIVDTTRKIRNGLSNIKGIKLQGPSDVCIVSWTTNDGVELYRFHNFMKEKHW 458
Query: 351 ---GISAHRGYIRIVFMPHVTKEMVEEFLRDLREVLK 384
G+ G +V M H + E F+ D R ++
Sbjct: 459 QLNGLQFPAGVHIMVTMNHTHPGLAEAFVADCRAAVE 495
>pir||G72753 hypothetical protein APE0020 - Aeropyrum pernix (strain K1)
>gi|5103408|dbj|BAA78929.1| (AP000058) 473aa long
hypothetical protein [Aeropyrum pernix]
Length = 473
Score = 148 bits (369), Expect = 1e-34
Identities = 106/349 (30%), Positives = 172/349 (48%), Gaps = 18/349 (5%)
Query: 52 YIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGY-GHIVSGGTEANILAVRA----FR 106
Y D+ + D ++P ++E++++ L H +GY G GGTE+ ILAV A +R
Sbjct: 55 YRDKTMLDFTVYPSIIELEKQLLGFAGHLFHAPEGYSGTFTYGGTESIILAVLAARERWR 114
Query: 107 NISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQ-DYAVDVKDVEAKISDNTIGIV 165
+ ++++P +AH +F KA +LG+K+ + D +E KI +T+ IV
Sbjct: 115 RAGKSGAGKIVMPITAHPAFAKAAYLLGLKVERVPVDSVTLQADPAIIEEKIDRDTVMIV 174
Query: 166 GIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVE 225
A G +D + L D+A + LHVDA GG V+ FA G ++ FDF ++GV
Sbjct: 175 ASAVDYPYGSLDPVEDLGDIAAARDVWLHVDACIGGMVLAFASDAGEEVGKFDFGVEGVR 234
Query: 226 SITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVW 285
S ++D HK G AP + ++FRR + K + G + I TR ++ A W
Sbjct: 235 SFSVDMHKYGYAPKGSSILLFRRARDKKPTIFVDSSWPGYPLVNQAILSTRSAGTLAAAW 294
Query: 286 ALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKNLRKVE--R 343
A+ + LG EGYRE+ +E R + ++ L L R P I+SF ++ VE
Sbjct: 295 AVARTLGVEGYRELAGMVLEARRRIQKGLESLGLEVLGR-PKAGILSFTDSDIDVVEVAT 353
Query: 344 ELKRRGWGI-----SAHRGY---IRIVFMPHVTKEMVEEFLRDLREVLK 384
L R GW + + H G+ I + P + +V+ FL + E ++
Sbjct: 354 RLGRAGWVVQLQPGNKHLGFPTSIHLTISP-IHARVVDSFLAAVEESIR 401
>pir||G72452 probable glutamate decarboxylase APE2267 - Aeropyrum pernix
(strain K1) >gi|5105968|dbj|BAA81279.1| (AP000064) 454aa
long hypothetical glutamate decarboxylase [Aeropyrum
pernix]
Length = 454
Score = 137 bits (342), Expect = 2e-31
Identities = 106/389 (27%), Positives = 180/389 (46%), Gaps = 22/389 (5%)
Query: 12 EEVLNLLEDKTKVDLTFSSGKILGSMCTMPH----ELAIEVFARYIDRNLGDPGLHPGTR 67
EEV+ L + +D G++ + E+++ F ++D N DP +
Sbjct: 2 EEVVKRLAEIRAMDARGEGGRLFTYLYETGDPGVKEVSLRAFEMFLDTNALDPTVFKSAL 61
Query: 68 KIEEEVIEMLSDLLHLEKGY-GHIVSGGTEANILAVRA----FRNISDAERPELILPKSA 122
E E++ S L +G G + GGTE+ ILA A +R++ + P ++ P++
Sbjct: 62 FFERELVSFASSLAGGVEGVVGTVTYGGTESIILAAMAAREWYRSLGGSRTPGIVAPQTV 121
Query: 123 HFSFIKAGEMLGVKLVWAELKQ-DYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPA 181
H S KA LG++L A + VD+ + + + D T +V A G VDD+ +
Sbjct: 122 HPSVRKAARYLGMRLSIAPVDPGSKRVDIDSLVSLVDDRTAMVVVSAPNYPYGTVDDVRS 181
Query: 182 LSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPA 241
+++ + LHVDA GGF++PF + LG F F ++GV S+++D HK G +P A
Sbjct: 182 VAEALSSQRVWLHVDACVGGFILPFMRELGLYSGAFAFDVEGVYSVSMDLHKYGYSPKGA 241
Query: 242 GGIIFRRKKYLKAISVLAPYLAGGKVW-QATITGTRPGASVLAVWALIKHLGFEGYREIV 300
++FR LK S+ A G + T+ +R A + A WA+ +LG GY E+
Sbjct: 242 SVLLFRDGS-LKKHSIFADLRWPGYPFINTTVLSSRSVAPLAAAWAVTNYLGRRGYLELA 300
Query: 301 RKAMELSRWFAEEIKKLNNAWL--VREPMLNIVSFQTKNLRKVERELKRRGWGISAHRGY 358
RKA+E ++ + L + +L++ + + + RRGW + G
Sbjct: 301 RKAVEARDEIMRGLESIGFRSLAPIESTILSVALDDPADTLRFHANMSRRGWILGLQPGV 360
Query: 359 -------IRIVFMPHVTKEMVEEFLRDLR 380
I + P + K + +FL D R
Sbjct: 361 EGLAPPNIHLTISP-IHKLVSPQFLGDAR 388
>sp|P54767|DCE_LYCES GLUTAMATE DECARBOXYLASE (GAD) (ERT D1) >gi|1362098|pir||S56177
probable glutamate decarboxylase - tomato
>gi|995555|emb|CAA56812.1| (X80840) homology to
pyroxidal-5'-phosphate-dependant glutamate
decarboxylases; putative start codon [Lycopersicon
esculentum]
Length = 502
Score = 122 bits (303), Expect = 7e-27
Identities = 105/417 (25%), Positives = 190/417 (45%), Gaps = 42/417 (10%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K P+K +P+E ++ D+ +D + + T ++ I++N D
Sbjct: 32 KMPKKSMPKEAAYQIVNDELMLDGN-PRLNLASFVSTWMEPECDKLIMSSINKNYVDMDE 90
Query: 63 HPGTRKIEEEVIEMLSDLLHL-----EKGYGHIVSGGTEANILAVRAFRNISDAER---- 113
+P T +++ + ML+ L H E G G +EA +LA AF+ ++R
Sbjct: 91 YPVTTELQNRCVNMLAHLFHAPVGDDETAVGVGTVGSSEAIMLAGLAFKRKWQSKRKAEG 150
Query: 114 -----PELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV--DVKDVEAKISDNTIGIVG 166
P ++ + + K V+L +LK+ Y V K VE + +NTI +
Sbjct: 151 KPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLKEGYYVMDPAKAVEI-VDENTICVAA 209
Query: 167 IAGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFK 220
I G+T G +D+ L++L + + P+HVDAA GGF+ PF L DL ++DF+
Sbjct: 210 ILGSTLTGEFEDVKLLNELLTKKNKETGWETPIHVDAASGGFIAPF---LWPDL-EWDFR 265
Query: 221 LKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGAS 280
L V+SI + HK G+ G +I+R K+ L V G T+ ++
Sbjct: 266 LPLVKSINVSGHKYGLVYAGVGWVIWRSKEDLPDELVFHINYLGSDQPTFTLNFSKGSYQ 325
Query: 281 VLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPM-LNIVSFQTKNLR 339
++A + + LGFEGY+ +++ + ++ E I K+ +V + + + +V+F ++
Sbjct: 326 IIAQYYQLIRLGFEGYKNVMKNCLSNAKVLTEGITKMGRFDIVSKDVGVPVVAFSLRDSS 385
Query: 340 K-----VERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVL 383
K V L+R GW + A H +R+V + + E + D+ ++L
Sbjct: 386 KYTVFEVSEHLRRFGWIVPAYTMPPDAEHIAVLRVVIREDFSHSLAERLVSDIEKIL 442
>pir||F70975 probable glutamate decarboxylase - Mycobacterium tuberculosis
(strain H37RV) >gi|2104353|emb|CAB08681.1| (Z95389) gadB
[Mycobacterium tuberculosis]
Length = 460
Score = 121 bits (301), Expect = 1e-26
Identities = 99/383 (25%), Positives = 176/383 (45%), Gaps = 37/383 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
+ P++ + E + D+ +D + S + + T A ++ A D+N+ D
Sbjct: 30 RMPDESMDPEAAYRFIHDELMLDGS-SRLNLATFVTTWMDPEAEKLMAETFDKNMIDKDE 88
Query: 63 HPGTRKIEEEVIEMLSDLLHLE--------KGYGHIVSGGTEANILAVRAFR-------N 107
+P T IE + M++DL H E G G +EA +L A +
Sbjct: 89 YPATAAIEARCVSMVADLFHAEGLRDHDPTSATGVSTIGSSEAVMLGGLALKWRWRQRVG 148
Query: 108 ISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQD-YAVDVKDVEAKISDNTIGIVG 166
P L++ + + K V+ + +++ Y + + V A + +NTIG+V
Sbjct: 149 SWKGRMPNLVMGSNVQVVWEKFCRYFDVEPRYLPMERGRYVITPEQVLAAVDENTIGVVA 208
Query: 167 IAGTTGLGVVDDIP----ALSDLAREYGI--PLHVDAAFGGFVIPFAKSLGYDLPD--FD 218
I GTT G ++ I AL LA G+ P+HVDAA GGFV+PF PD +D
Sbjct: 209 ILGTTYTGELEPIAEICAALDKLAAGGGVDVPVHVDAASGGFVVPFLH------PDLVWD 262
Query: 219 FKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPG 278
F+L V SI + HK G+ G +++R ++L V GG + T+ +RPG
Sbjct: 263 FRLPRVVSINVSGHKYGLTYPGVGFVVWRGPEHLPEDLVFRVNYLGGDMPTFTLNFSRPG 322
Query: 279 ASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVRE-PMLNIVSFQTKN 337
V+ + LG +GY ++++ +RW ++++++++ ++ + + +VSF+
Sbjct: 323 NQVVGQYYNFLRLGRDGYTKVMQALSHTARWLGDQLREVDHCEVISDGSAIPVVSFRLAG 382
Query: 338 LR-----KVERELKRRGWGISAH 355
R V EL+ GW + A+
Sbjct: 383 DRGYTEFDVSHELRTFGWQVPAY 405
>dbj|BAA88152.1| (AB025422) glutamic acid decarboxylase [Aspergillus oryzae]
Length = 514
Score = 121 bits (300), Expect = 2e-26
Identities = 101/418 (24%), Positives = 185/418 (44%), Gaps = 40/418 (9%)
Query: 2 SKFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPG 61
++ PE+ +PRE +++D+ +D + + T + A ++ +N D
Sbjct: 50 TEMPEREMPREVAYRMIKDELSLDGN-PMLNLASFVTTYMEDEAEKLMTESFSKNFIDYE 108
Query: 62 LHPGTRKIEEEVIEMLSDLLHL------EKGYGHIVSGGTEANILAVRAFRNI------- 108
+P + +I+ + M++ L + E G G +EA +L A +
Sbjct: 109 EYPQSAEIQNRCVNMIARLFNAPVHSEDEHPMGTSTIGSSEAIMLGTLAMKRRWQNKRKA 168
Query: 109 --SDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQD-YAVDVKDVEAKISDNTIGIV 165
D RP +++ + + KA V+ + +D Y +D + + +NTIGI
Sbjct: 169 EGKDYSRPNIVMNSAVQVCWEKAARYFDVEERYVYCTEDRYVIDPQQAVDLVDENTIGIC 228
Query: 166 GIAGTTGLGVVDDIPALSDLA--REYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKG 223
I GTT G +D+ A++DL R +P+HVDAA GGFV PF ++DF+L
Sbjct: 229 AILGTTYTGEYEDVKAINDLLIERNIDVPIHVDAASGGFVAPFINPK----LEWDFRLPK 284
Query: 224 VESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLA 283
V SI + HK G+ G +++R +YL + G + T+ ++ + V+
Sbjct: 285 VVSINVSGHKYGLVYPGVGWVVWRSPEYLPKDLIFNINYLGAEQASFTLNFSKGASQVIG 344
Query: 284 VWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV--REPMLNIVSFQTKNLRK- 340
+ + LG GYR I+ + + A+E++K+ + R L +V+F+ R+
Sbjct: 345 QYYQMIRLGKRGYRSIMTNITVTADFLAQELEKMGFIIMSQRRGHGLPLVAFRLPAEREG 404
Query: 341 ------VERELKRRGWGISAHR--------GYIRIVFMPHVTKEMVEEFLRDLREVLK 384
+ +L+ RGW + A+ +R+V TK + L D++ LK
Sbjct: 405 QFDEFALAHQLRERGWIVPAYTMAPNSNNLKLMRVVVREDFTKSRCDALLSDIKLGLK 462
>gb|AAC24195.1| (AF020425) glutamate decarboxylase isozyme 1 [Nicotiana tabacum]
Length = 496
Score = 118 bits (293), Expect = 1e-25
Identities = 102/416 (24%), Positives = 183/416 (43%), Gaps = 40/416 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K PE +P+E ++ D+ +D + + T ++ I++N D
Sbjct: 31 KMPENSIPKEAAYQIINDELMLDGN-PRLNLASFVTTWMEPECNKLMMDSINKNYVDMDE 89
Query: 63 HPGTRKIEEEVIEMLSDLLHLEKGYGHIVSG-----GTEANILAVRAFRNI--------- 108
+P T +++ + M++ L + G G G +EA +LA AF+
Sbjct: 90 YPVTTELQNRCVNMIAHLFNAPLGDGETAVGVGTVGSSEAIMLAGLAFKRKWQNKMKAQG 149
Query: 109 SDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
++P ++ + + K V+L +L Y V D + + +NTI + I
Sbjct: 150 KPCDKPNIVTGANVQVCWEKFARYFEVELKEVKLSDGYYVMDPEKAVEMVDENTICVAAI 209
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+L
Sbjct: 210 LGSTLNGEFEDVKRLNDLLIEKNKETGWDTPIHVDAASGGFIAPFL----YPELEWDFRL 265
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
V+SI + HK G+ G I+R K+ L + G T+ ++ + V
Sbjct: 266 PLVKSINVSGHKYGLVYAGIGWAIWRNKEDLPDELIFHINYLGADQPTFTLNFSKGSSQV 325
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTK---- 336
+A + + LGFEGY+ ++ E +R E ++K ++ +E + +V+F K
Sbjct: 326 IAQYYQLIRLGFEGYKNVMENCQENARVLREGLEKSGRFNIISKEIGVPLVAFSLKDNSQ 385
Query: 337 -NLRKVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVL 383
N ++ L+R GW I A H +R+V ++ + E + D+ +VL
Sbjct: 386 HNEFEISETLRRFGWIIPAYTMPPNAQHVTVLRVVIREDFSRTLAERLVIDIEKVL 441
>gb|AAD20099.1| (AC006532) putative glutamate decarboxylase [Arabidopsis thaliana]
Length = 493
Score = 118 bits (293), Expect = 1e-25
Identities = 105/417 (25%), Positives = 184/417 (43%), Gaps = 42/417 (10%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
+ PE +P+E ++ D+ +D + + T ++ I++N D
Sbjct: 31 EMPENSIPKEAAYQIINDELMLDGN-PRLNLASFVTTWMEPECDKLMMESINKNYVDMDE 89
Query: 63 HPGTRKIEEEVIEMLSDLLHLEKGYGHIVSG-----GTEANILAVRAFRNISDAER---- 113
+P T +++ + M++ L + G G G +EA +LA AF+ +R
Sbjct: 90 YPVTTELQNRCVNMIARLFNAPLGDGEAAVGVGTVGSSEAIMLAGLAFKRQWQNKRKAQG 149
Query: 114 -----PELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV--DVKDVEAKISDNTIGIVG 166
P ++ + + K V+L L++DY V VK VE + +NTI +
Sbjct: 150 LPYDKPNIVTGANVQVCWEKFARYFEVELKEVNLREDYYVMDPVKAVEM-VDENTICVAA 208
Query: 167 IAGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFK 220
I G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+
Sbjct: 209 ILGSTLTGEFEDVKLLNDLLVEKNKQTGWDTPIHVDAASGGFIAPFL----YPELEWDFR 264
Query: 221 LKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGAS 280
L V+SI + HK G+ G +++R K L + G T+ ++ +
Sbjct: 265 LPLVKSINVSGHKYGLVYAGIGWVVWRTKTDLPDELIFHINYLGADQPTFTLNFSKGSSQ 324
Query: 281 VLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTK--- 336
V+A + + LGFEGYR ++ E + ++K +V +E + +V+F K
Sbjct: 325 VIAQYYQLIRLGFEGYRNVMDNCRENMMVLRQGLEKTGRFKIVSKENGVPLVAFSLKDSS 384
Query: 337 --NLRKVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVL 383
N +V L+R GW + A H +R+V ++ + E + D +VL
Sbjct: 385 RHNEFEVAHTLRRFGWIVPAYTMPADAQHVTVLRVVIREDFSRTLAERLVADFEKVL 441
>sp|Q07346|DCE_PETHY GLUTAMATE DECARBOXYLASE (GAD) >gi|1076648|pir||A48767 glutamate
decarboxylase (EC 4.1.1.15), calmodulin-binding - garden
petunia >gi|294112|gb|AAA33709.1| (L16797) glutamate
decarboxylase [Petunia x hybrida]
>gi|309680|gb|AAA33710.1| (L16977) glutamate
decarboxylase [Petunia x hybrida]
Length = 500
Score = 118 bits (292), Expect = 1e-25
Identities = 98/416 (23%), Positives = 185/416 (43%), Gaps = 40/416 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K P+ +P+E ++ D+ +D + + T ++ I++N D
Sbjct: 31 KMPDNSIPKEAAYQIINDELMLDGN-PRLNLASFVTTWMEPECDKLMMDSINKNYVDMDE 89
Query: 63 HPGTRKIEEEVIEMLSDLLHL-----EKGYGHIVSGGTEANILAVRAFRNI--------- 108
+P T +++ + M++ L + E G G +EA +LA AF+
Sbjct: 90 YPVTTELQNRCVNMIAHLFNAPLEDGETAVGVGTVGSSEAIMLAGLAFKRKWQNKMKAQG 149
Query: 109 SDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
++P ++ + + K V+L +L + Y V D + + +NTI + I
Sbjct: 150 KPCDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPEKAVEMVDENTICVAAI 209
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+L
Sbjct: 210 LGSTLNGEFEDVKRLNDLLVEKNKETGWDTPIHVDAASGGFIAPFI----YPELEWDFRL 265
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
V+SI + HK G+ G +++R K L + G T+ ++ + V
Sbjct: 266 PLVKSINVSGHKYGLVYAGIGWVVWRNKDDLPDELIFHINYLGADQPTFTLNFSKGSSQV 325
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTKNLR- 339
+A + + LG+EGY+ ++ E + E ++K ++ +E + +V+F K+ R
Sbjct: 326 IAQYYQLIRLGYEGYKNVMENCQENASVLREGLEKTGRFNIISKEIGVPLVAFSLKDNRQ 385
Query: 340 ----KVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVL 383
++ L+R GW + A H +R+V ++ + E +RD+ +VL
Sbjct: 386 HNEFEISETLRRFGWIVPAYTMPPNAQHITVLRVVIREDFSRTLAERLVRDIEKVL 441
>dbj|BAB02870.1| (AB026646) glutamate decarboxylase [Arabidopsis thaliana]
Length = 494
Score = 117 bits (291), Expect = 2e-25
Identities = 100/417 (23%), Positives = 183/417 (42%), Gaps = 40/417 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K P+ +P++ ++ D+ +D + + T ++ +++N D
Sbjct: 30 KMPDHCMPKDAAYQVINDELMLDGN-PRLNLASFVTTWMEPECDKLIMDSVNKNYVDMDE 88
Query: 63 HPGTRKIEEEVIEMLSDLLHL-----EKGYGHIVSGGTEANILAVRAFRNISDAER---- 113
+P T +++ + M+++L H E G G +EA +LA AF+ R
Sbjct: 89 YPVTTELQNRCVNMIANLFHAPVGEDEAAIGCGTVGSSEAIMLAGLAFKRKWQHRRKAQG 148
Query: 114 -----PELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
P ++ + + K V+L +L +DY V D + +NTI + I
Sbjct: 149 LPIDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEDYYVMDPAKAVEMVDENTICVAAI 208
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+L
Sbjct: 209 LGSTLTGEFEDVKQLNDLLAEKNAETGWETPIHVDAASGGFIAPFL----YPDLEWDFRL 264
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
V+SI + HK G+ G +++R K L V G T+ ++ + +
Sbjct: 265 PWVKSINVSGHKYGLVYAGVGWVVWRTKDDLPEELVFHINYLGADQPTFTLNFSKGSSQI 324
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPM-LNIVSFQTKNLRK 340
+A + LGFEGY+ I+ M+ +R E I+ +V + + + +V+F K+ K
Sbjct: 325 IAQYYQFIRLGFEGYKNIMENCMDNARRLREGIEMTGKFNIVSKDIGVPLVAFSLKDSSK 384
Query: 341 -----VERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVLK 384
+ L++ GW I A H +R+V ++ + + + + +VLK
Sbjct: 385 HTVFEIAESLRKFGWIIPAYTMPADAQHIAVLRVVIREDFSRGLADRLITHIIQVLK 441
>gb|AAC46188.1| (AF005098) glutamate decarboxylase [Lactococcus lactis]
Length = 466
Score = 117 bits (290), Expect = 2e-25
Identities = 91/340 (26%), Positives = 166/340 (48%), Gaps = 33/340 (9%)
Query: 45 AIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHL-EKG--YGHIVSGGTEANILA 101
A+++ ++ +++N D +P T +IE + M++DL + EKG YG G +EA +L
Sbjct: 72 AVKLMSQTLEKNAIDKSEYPRTTEIENRCVNMIADLWNASEKGKIYGTSTIGSSEACMLG 131
Query: 102 VRAFRNI-----------SDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDY-AVD 149
A + +A++P L++ + K +++ + +++ +++
Sbjct: 132 GMAMKFSWRKRAEKLGLDINAKKPNLVISSGYQVCWEKFCVYWDIEMREVPMDREHMSIN 191
Query: 150 VKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYG------IPLHVDAAFGGFV 203
++ V + + TIG+VGI G T G DDI AL +L EY + +HVDAA GG
Sbjct: 192 LEKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVYIHVDAASGGLY 251
Query: 204 IPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLA 263
PF + +L ++DF+LK V SI HK G+ G +++R KKYL +
Sbjct: 252 APFVEP---EL-EWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYL 307
Query: 264 GGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV 323
GG++ I + + ++ + GF+GY+ I + +++ + AEEI+K ++
Sbjct: 308 GGELPTMAINFSHSASQLIGQYYNFVRYGFDGYKAIHERTHKVAMYLAEEIEKTGMFEIM 367
Query: 324 RE-PMLNIVSFQTK-------NLRKVERELKRRGWGISAH 355
+ L IV ++ K NL + L +GW + A+
Sbjct: 368 NDGAQLPIVCYKLKENSNRGWNLYDLADRLLMKGWQVPAY 407
>pir||T49478 probable glutamate decarboxylase [imported] - Neurospora crassa
>gi|7899334|emb|CAB91739.1| (AL356173) probable
glutamate decarboxylase [Neurospora crassa]
Length = 520
Score = 116 bits (289), Expect = 3e-25
Identities = 104/416 (25%), Positives = 183/416 (43%), Gaps = 45/416 (10%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
+ P++ +P+E +++D+ +D + + T E A ++ + +N D
Sbjct: 57 EMPDEEMPKEVAYRMIKDELSLDGN-PLLNLASFVTTYMEEEAEKLMTESLPKNFIDYEE 115
Query: 63 HPGTRKIEEEVIEMLSDLLHL-------EKGYGHIVSGGTEANILAVRAFRNI------- 108
+P T I+ + M+ L + G G +EA +L V A +
Sbjct: 116 YPQTADIQNRCVSMIGRLFNAPVKDAEASSAVGTSSVGSSEAIMLGVLAMKKRWKNKRIA 175
Query: 109 --SDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQD-YAVDVKDVEAKISDNTIGIV 165
A++P LI+ + + KA V+ + D Y +D K+ + +NTIGI
Sbjct: 176 EGKPADKPNLIMSSAVQVCWEKATRYFEVEEKFVYCTPDRYVIDPKETVDLVDENTIGIC 235
Query: 166 GIAGTTGLGVVDDIPALSDLAREYGI--PLHVDAAFGGFVIPFAKSLGYDLPD--FDFKL 221
I GTT G +D+ A++DL E G+ P+HVDAA GGFV PF +PD +DF+L
Sbjct: 236 CILGTTYTGEYEDVKAVNDLLVERGLDTPIHVDAASGGFVAPFV------VPDLEWDFRL 289
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
K V SI + HK G+ G +++R +YL V G T+ ++ + V
Sbjct: 290 KNVVSINVSGHKYGLVYPGVGWVVWRSAEYLPQELVFNINYLGADQASFTLNFSKGASQV 349
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVRE--PMLNIVSFQTK--- 336
+ + + LG GYR I+ + + AE + L + ++ L +V+F+ K
Sbjct: 350 IGQYYQLIRLGKHGYRAIMSNLTRTADYLAESLAALGFIIMSQKSGQGLPLVAFRLKEDP 409
Query: 337 ----NLRKVERELKRRGWGISAHR--------GYIRIVFMPHVTKEMVEEFLRDLR 380
+ + +L+ RGW + A+ +RIV ++ + + D+R
Sbjct: 410 DRTYDEFALAHQLRVRGWIVPAYTMAPKTEGLKMLRIVVREDFSRNRCDGLISDIR 465
>gb|AAB40608.1| (U54774) glutamate decarboxylase [Nicotiana tabacum]
Length = 496
Score = 115 bits (285), Expect = 9e-25
Identities = 101/416 (24%), Positives = 182/416 (43%), Gaps = 40/416 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K PE +P+E ++ D+ +D + + T ++ I++N D
Sbjct: 31 KMPENSIPKEAAYQIINDELMLDGN-PRLNLASFVTTWMEPECNKLMMDSINKNYVDMDE 89
Query: 63 HPGTRKIEEEVIEMLSDLLHLEKGYGHIVSG-----GTEANILAVRAFRNI--------- 108
+P T +++ + M++ L + G G G +EA +LA AF+
Sbjct: 90 YPVTTELQNRCVNMIAHLFNAPLGDGETAVGVGTVGSSEAIMLAGLAFKRKWQNKMKAQG 149
Query: 109 SDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
++P ++ + + K V+L +L Y V D + + +NTI + I
Sbjct: 150 KPCDKPNIVTGANVQVCWEKFARYFEVELKEVKLSDGYYVMDPEKAVEMVDENTICVAAI 209
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA G F+ PF Y ++DF+L
Sbjct: 210 LGSTLNGEFEDVKRLNDLLIEKNKETGWDTPIHVDAASGEFIAPFL----YPELEWDFRL 265
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
V+SI + HK G+ G I+R K+ L + G T+ ++ + V
Sbjct: 266 PLVKSINVSGHKYGLVYAGIGWAIWRNKEDLPDELIFHINYLGADQPTFTLNFSKGSSQV 325
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTK---- 336
+A + + LGFEGY+ ++ E +R E ++K ++ +E + +V+F K
Sbjct: 326 IAQYYQLIRLGFEGYKNVMENCQENARVLREGLEKSGRFNIISKEIGVPLVAFSLKDNSQ 385
Query: 337 -NLRKVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVL 383
N ++ L+R GW I A H +R+V ++ + E + D+ +VL
Sbjct: 386 HNEFEISETLRRFGWIIPAYTMPPNAQHVTVLRVVIREDFSRTLAERLVIDIEKVL 441
>dbj|BAB10520.1| (AB005238) glutamate decarboxylase 1 (GAD 1) [Arabidopsis thaliana]
Length = 502
Score = 113 bits (280), Expect = 4e-24
Identities = 101/417 (24%), Positives = 185/417 (44%), Gaps = 40/417 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K PE +P+E ++ D+ +D + + T ++ I++N D
Sbjct: 31 KMPENSIPKEAAYQIINDELMLDGN-PRLNLASFVTTWMEPECDKLIMSSINKNYVDMDE 89
Query: 63 HPGTRKIEEEVIEMLSDLLHL-----EKGYGHIVSGGTEANILAVRAFR----NISDAE- 112
+P T +++ + M++ L + E G G +EA +LA AF+ N AE
Sbjct: 90 YPVTTELQNRCVNMIAHLFNAPLEEAETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEG 149
Query: 113 ----RPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
+P ++ + + K V+L +L + Y V D + + +NTI + I
Sbjct: 150 KPVDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPQQAVDMVDENTICVAAI 209
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+L
Sbjct: 210 LGSTLNGEFEDVKLLNDLLVEKNKETGWDTPIHVDAASGGFIAPFL----YPELEWDFRL 265
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
V+SI + HK G+ G +I+R K+ L + G T+ ++ + V
Sbjct: 266 PLVKSINVSGHKYGLVYAGIGWVIWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQV 325
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTKNLR- 339
+A + + LG EGYR ++ E E ++K +V ++ + +V+F K+
Sbjct: 326 IAQYYQLIRLGHEGYRNVMENCRENMIVLREGLEKTERFNIVSKDEGVPLVAFSLKDSSC 385
Query: 340 ----KVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVLK 384
++ L+R GW + A H +R+V ++ + E + D+ +V++
Sbjct: 386 HTEFEISDMLRRYGWIVPAYTMPPNAQHITVLRVVIREDFSRTLAERLVIDIEKVMR 442
>gb|AAD20093.1| (AC006532) putative glutamate decarboxylase [Arabidopsis thaliana]
Length = 500
Score = 113 bits (279), Expect = 5e-24
Identities = 103/416 (24%), Positives = 182/416 (42%), Gaps = 40/416 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
+ P+ +P+E ++ D+ K D + + T ++ I++N +
Sbjct: 31 EIPKNSIPKEAAYQIINDELKFDGN-PRLNLASFVTTWMEPECDKLMMESINKNNVEMDQ 89
Query: 63 HPGTRKIEEEVIEMLSDLLHLEKGYGHIVSG-----GTEANILAVRAFR----NISDA-- 111
+P T ++ + M++ L + G G G +EA +LA AF+ N A
Sbjct: 90 YPVTTDLQNRCVNMIARLFNAPLGDGEAAIGVGTVGSSEAVMLAGLAFKRQWQNKRKALG 149
Query: 112 ---ERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
+RP ++ + K V+L +L++ Y V D + +NTI +V I
Sbjct: 150 LPYDRPNIVTGANIQVCLEKFARYFEVELKEVKLREGYYVMDPDKAVEMVDENTICVVAI 209
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+L
Sbjct: 210 LGSTLTGEFEDVKLLNDLLVEKNKKTGWDTPIHVDAASGGFIAPFL----YPDLEWDFRL 265
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
V+SI + HK G+ G +++R K L + G T+ ++ + V
Sbjct: 266 PLVKSINVSGHKYGLVYAGIGWVVWRTKTDLPDELIFHINYLGADQPTFTLNFSKGSSQV 325
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTK---- 336
+A + + LGFEGYR ++ E + ++K +V +E + +V+F K
Sbjct: 326 IAQYYQLIRLGFEGYRNVMDNCRENMMVLRQGLEKTGRFNIVSKENGVPLVAFSLKDSSR 385
Query: 337 -NLRKVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVL 383
N +V L+R GW + A H +R+V ++ + E + D +VL
Sbjct: 386 HNEFEVAEMLRRFGWIVPAYTMPADAQHVTVLRVVIREDFSRTLAERLVADFEKVL 441
>sp|Q42521|DCE1_ARATH GLUTAMATE DECARBOXYLASE 1 (GAD 1) >gi|497979|gb|AAA93132.1|
(U10034) glutamate decarboxylase [Arabidopsis thaliana]
Length = 502
Score = 113 bits (279), Expect = 5e-24
Identities = 101/417 (24%), Positives = 185/417 (44%), Gaps = 40/417 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K PE +P+E ++ D+ +D + + T ++ I++N D
Sbjct: 31 KMPENSIPKEAAYQIINDELMLDGN-PRLNLASFVTTWMEPECDKLIMSSINKNYVDMDE 89
Query: 63 HPGTRKIEEEVIEMLSDLLHL-----EKGYGHIVSGGTEANILAVRAFR----NISDAE- 112
+P T +++ + M++ L + E G G +EA +LA AF+ N AE
Sbjct: 90 YPVTTELQNRCVNMIAHLFNAPLEEAETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEG 149
Query: 113 ----RPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
+P ++ + + K V+L +L + Y V D + + +NTI + I
Sbjct: 150 KPVDKPNIVTGANVQVCWEKFARYFEVELKEVKLSEGYYVMDPQQAVDMVDENTICVADI 209
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+L
Sbjct: 210 LGSTLNGEFEDVKLLNDLLVEKNKETGWDTPIHVDAASGGFIAPFL----YPELEWDFRL 265
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
V+SI + HK G+ G +I+R K+ L + G T+ ++ + V
Sbjct: 266 PLVKSINVSGHKYGLVYAGIGWVIWRNKEDLPEELIFHINYLGADQPTFTLNFSKGSSQV 325
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTKNLR- 339
+A + + LG EGYR ++ E E ++K +V ++ + +V+F K+
Sbjct: 326 IAQYYQLIRLGHEGYRNVMENCRENMIVLREGLEKTERFNIVSKDEGVPLVAFSLKDSSC 385
Query: 340 ----KVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVLK 384
++ L+R GW + A H +R+V ++ + E + D+ +V++
Sbjct: 386 HTEFEISDMLRRYGWIVPAYTMPPNAQHITVLRVVIREDFSRTLAERLVIDIEKVMR 442
>pir||T01962 glutamate decarboxylase (EC 4.1.1.15) 2, calmodulin-binding -
common tobacco >gi|3252854|gb|AAC39483.1| (AF020424)
glutamate decarboxylase isozyme 2 [Nicotiana tabacum]
Length = 496
Score = 113 bits (279), Expect = 5e-24
Identities = 100/415 (24%), Positives = 180/415 (43%), Gaps = 40/415 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
K PE +P+E ++ D+ +D + + T + I++N D
Sbjct: 31 KMPENSIPKEAAYQIINDELMLDGN-PRLNLASFVTTWMEPECNTLMMDSINKNYVDMDE 89
Query: 63 HPGTRKIEEEVIEMLSDLLHLEKGYGHIVSG-----GTEANILAVRAFRNI--------- 108
+P T +++ + M++ L + G G G +EA +LA AF+
Sbjct: 90 YPVTTELQNRCVNMIAHLFNAPLGDGETAVGVGTVGSSEAIMLAGLAFKRKWQNKMKAQG 149
Query: 109 SDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAV-DVKDVEAKISDNTIGIVGI 167
++P ++ + + K V+L +L Y V D + + +NTI + I
Sbjct: 150 KPFDKPNIVTGANVQVCWEKFARYFEVELKEVKLSDGYYVMDPEKAVEMVDENTICVAAI 209
Query: 168 AGTTGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKL 221
G+T G +D+ L+DL E + P+HVDAA GGF+ PF Y ++DF+L
Sbjct: 210 LGSTLNGEFEDVKRLNDLLIEKNKETGWDTPIHVDAASGGFIAPFL----YPELEWDFRL 265
Query: 222 KGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASV 281
+SI + HK G+ G I+R K+ L + G T+ ++ + V
Sbjct: 266 PLEKSINVSGHKYGLVYAGIGWAIWRNKEDLPDELIFHINYLGADQPTFTLNFSKGSSQV 325
Query: 282 LAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTK---- 336
+A + + LGFEGY+ ++ E +R E I+K ++ +E + +V+F K
Sbjct: 326 IAQYYQLIRLGFEGYKNVMENCQENARVLREGIEKSGRFNIISKEIGVPLVAFSLKDNSQ 385
Query: 337 -NLRKVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREV 382
N ++ L+R GW + A H +R+V ++ + E + D+ +V
Sbjct: 386 HNEFEISETLRRFGWIVLAYTMPPNAQHVTVLRVVIREDFSRTLAERLVIDIEKV 440
>dbj|BAA24585.1| (AB010789) glutamate decarboxylase [Lactococcus lactis]
Length = 466
Score = 112 bits (278), Expect = 6e-24
Identities = 94/377 (24%), Positives = 175/377 (45%), Gaps = 41/377 (10%)
Query: 45 AIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHL---EKGYGHIVSGGTEANILA 101
A+++ ++ +++N D +P T +IE + M++DL + EK G G +EA +L
Sbjct: 72 AVKLMSQTLEKNAIDKSEYPRTTEIENRCVNMIADLWNASEKEKFMGTSTIGSSEACMLG 131
Query: 102 VRAFRNI-----------SDAERPELILPKSAHFSFIKAGEMLGVKLVWAEL-KQDYAVD 149
A + +A++P L++ + K +++ + K+ +++
Sbjct: 132 GMAMKFSWRKRAEKLGLDINAKKPNLVISSGYQVCWEKFCIYWDIEMREVPMDKEHMSIN 191
Query: 150 VKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYG------IPLHVDAAFGGFV 203
+ V + + TIG+VGI G T G DDI AL +L EY + +HVDAA GG
Sbjct: 192 LDKVMDYVDEYTIGVVGIMGITYTGRYDDIKALDNLIEEYNKQTDYKVYIHVDAASGGLY 251
Query: 204 IPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLA 263
PF + +L ++DF+LK V SI HK G+ G +++R KKYL +
Sbjct: 252 APFVEP---EL-EWDFRLKNVISINTSGHKYGLVYPGVGWVLWRDKKYLPEELIFKVSYL 307
Query: 264 GGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV 323
GG++ I + + ++ + GF+GY+ I + +++ + A+EI+K ++
Sbjct: 308 GGELPTMAINFSHSASQLIGQYYNFVRYGFDGYKAIHERTHKVAMFLAKEIEKTGMFEIM 367
Query: 324 RE-PMLNIVSFQTK-------NLRKVERELKRRGWGISAH--------RGYIRIVFMPHV 367
+ L IV ++ K NL + L +GW + A+ R+V
Sbjct: 368 NDGSQLPIVCYKLKEDSNRGWNLYDLADRLLMKGWQVPAYPLPKNLENEIIQRLVIRADF 427
Query: 368 TKEMVEEFLRDLREVLK 384
M +++D++E ++
Sbjct: 428 GMNMAFNYVQDMQEAIE 444
>sp|P05034|DCHS_MORMO HISTIDINE DECARBOXYLASE (HDC) >gi|79014|pir||A25013 histidine
decarboxylase (EC 4.1.1.22) - Morganella morganii
>gi|149859|gb|AAA25321.1| (J02577) Histidine
decarboxylase [Morganella morganii]
Length = 378
Score = 112 bits (277), Expect = 8e-24
Identities = 86/291 (29%), Positives = 143/291 (48%), Gaps = 26/291 (8%)
Query: 46 IEVFARYIDRNLGDPGLHPG----TRKIEEEVIEMLSDLLHL--EKGYGHIVSGGTEANI 99
+E F R+ N GD G + + E+EV+E +DL + E+ +G++ +GGTE N+
Sbjct: 39 LERFLRFSINNCGDWGEYCNYLLNSFDFEKEVMEYFADLFKIPFEQSWGYVTNGGTEGNM 98
Query: 100 LAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISD 159
R I L K H+S K ++L +K E + + +D D+ KI+D
Sbjct: 99 FGCYLGREIFPDGT--LYYSKDTHYSVAKIVKLLRIKSQVVESQPNGEIDYDDLMKKIAD 156
Query: 160 NTIG---IVGIAGTTGLGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFAKSLG 211
+ I GTT G +DDI + + GI LH DAA G ++PF
Sbjct: 157 DKEAHPIIFANIGTTVRGAIDDIAEIQKRLKAAGIKREDYYLHADAALSGMILPFVD--- 213
Query: 212 YDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQAT 271
D F F G++SI + HKM +PIP G I+ +K+ + ISV Y++ T
Sbjct: 214 -DAQPFTFA-DGIDSIGVSGHKMIGSPIPCG-IVVAKKENVDRISVEIDYISAH---DKT 267
Query: 272 ITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLN-NAW 321
ITG+R G + L +W I+ E ++ + ++++++++ + ++K NAW
Sbjct: 268 ITGSRNGHTPLMLWEAIRSHSTEEWKRRITRSLDMAQYAVDRMQKAGINAW 318
>pir||T36342 probable glutamate decarboxylase - Streptomyces coelicolor
>gi|4808355|emb|CAB42769.1| (AL049841) putative
glutamate decarboxylase [Streptomyces coelicolor A3(2)]
Length = 475
Score = 112 bits (277), Expect = 8e-24
Identities = 114/416 (27%), Positives = 183/416 (43%), Gaps = 40/416 (9%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGL 62
+ P+ LP E L+ D+ +D + + + T A + + D+N+ D
Sbjct: 43 RLPDSPLPPESAYRLVHDELMLDGN-ARLNLATFVTTWMEPQAGVLMSECRDKNMIDKDE 101
Query: 63 HPGTRKIEEEVIEMLSDLLHL---EKGYGHIVSGGTEANILAVRAFRN---ISDAER--- 113
+P T ++E + ML+DL H G +G +EA +LA A + + +A+R
Sbjct: 102 YPRTAELERRCVAMLADLWHAPDPSTAVGCSTTGSSEACMLAGMALKRRWALRNADRYPA 161
Query: 114 ----PELILPKSAHFSFIKAGEMLGVKLVWAELKQD-YAVDVKDVEAKISDNTIGIVGIA 168
P L++ + + K V+ ++ D + +D + +NTIG+VGI
Sbjct: 162 KDVRPNLVMGVNVQVCWDKFCNFWEVEARQVPMEGDRFHLDPQAAAELCDENTIGVVGIL 221
Query: 169 GTTGLG----VVDDIPALSDLAREYG--IPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLK 222
G+T G V D AL L G +P+HVD A G V PF L DL +DF+L
Sbjct: 222 GSTFDGSYEPVADLCAALDALQERTGLDVPVHVDGASGAMVAPF---LDEDLV-WDFRLP 277
Query: 223 GVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVL 282
V SI HK G+ G ++R + L V GG + + +RPGA V+
Sbjct: 278 RVASINTSGHKYGLVYPGVGWALWRDAEALPEELVFRVNYLGGDMPTFALNFSRPGAQVV 337
Query: 283 AVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNN-AWLVREPMLNIVSFQTK----- 336
A + LG EGYR + + A +++ AE + L + L R L + +F T
Sbjct: 338 AQYYNFLRLGREGYRAVQQSARDIAGSLAERVAALGDFRLLTRGDQLPVFAFTTADDVTA 397
Query: 337 -NLRKVERELKRRGWGISA-----HR---GYIRIVFMPHVTKEMVEEFLRDLREVL 383
++ V R L+ GW + A HR +R+V + +M + L DL +L
Sbjct: 398 YDVFDVSRRLREGGWLVPAYTFPPHREDLSVLRVVCRNGFSADMADLLLADLERLL 453
>dbj|BAA78331.1| (AB014076) histidine decarboxylase [Brassica napus]
Length = 490
Score = 110 bits (273), Expect = 2e-23
Identities = 98/344 (28%), Positives = 160/344 (46%), Gaps = 39/344 (11%)
Query: 56 NLGDP------GLHPGTRKIEEEVIEMLSDLLHLEKG--YGHIVSGGTEANILAVRAFRN 107
NLGDP G+H +R E V++ + L +E+ +G+I + GTE N+ + R
Sbjct: 138 NLGDPFIESNYGVH--SRPFEVGVLDWFARLWEIERDDYWGYITNCGTEGNLHGILVGRE 195
Query: 108 ISDAERPELILPKSA--HFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTI--G 163
+ P+ IL S+ H+S KA M ++ + +D D K+ N
Sbjct: 196 VF----PDGILYASSESHYSVFKAARMYRMECEKVDTLISGEIDCDDFRRKLLANKDKPA 251
Query: 164 IVGI-AGTTGLGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFAKSLGYDLPDF 217
I+ + GTT G VDD+ + E G +H D A G ++PF K P
Sbjct: 252 ILNVNIGTTVKGAVDDLDLVIKTLEECGFSHDRFYIHCDGALFGLMMPFVKRA----PKV 307
Query: 218 DFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRP 277
F K + S+++ HK P+P G + R K++K +S YLA ATI G+R
Sbjct: 308 TFN-KPIGSVSVSGHKFVGCPMPCG-VQITRMKHIKVLSNNVEYLASR---DATIMGSRN 362
Query: 278 GASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKN 337
G + L +W + G++G+++ V+K + + + + +++ + MLN +S
Sbjct: 363 GHAPLFLWYTLNRKGYKGFQKEVQKCLRNAHYLKDRLREAGIS-----AMLNELSSTVVF 417
Query: 338 LRKVERELKRRGWGISAHRGYIRIVFMPHVTKEMVEEFLRDLRE 381
R E E RR W ++ +V MP VT E ++ FL+DL E
Sbjct: 418 ERPKEEEFVRR-WQLACQGDIAHVVVMPSVTVEKLDHFLKDLVE 460
>pir||S75150 glutamate decarboxylase - Synechocystis sp. (strain PCC 6803)
>gi|1652140|dbj|BAA17064.1| (D90903) glutamate
decarboxylase [Synechocystis sp.]
Length = 467
Score = 110 bits (272), Expect = 3e-23
Identities = 111/419 (26%), Positives = 189/419 (44%), Gaps = 59/419 (14%)
Query: 9 LPREEVL-----NLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGLH 63
+P E+L NL+ D+ +D S + + T A ++ A D+N+ D +
Sbjct: 36 MPETEMLPAIAYNLIHDELGLDGN-SRLNLATFVTTWMEPEARQLMADTFDKNMIDKDEY 94
Query: 64 PGTRKIEEEVIEMLSDLLHLE---KGYGHIVSGGTEANILAVRAFR---------NISDA 111
P T +IE + +LS L + + G G +EA +L A +
Sbjct: 95 PQTAEIELRCVNILSRLWNAPASAEATGCSTIGSSEAAMLGGMAMKWKWRQRRQAAGKPG 154
Query: 112 ERPELILPKSAHFSFIKAGEMLGVKLVWAELKQD-YAVDVKDVEAKISDNTIGIVGIAGT 170
+RP L++ + + K V+ + ++ D Y + ++ I +NTIG++GI G+
Sbjct: 155 DRPNLVMGINVQVCWEKFCRYWEVEPRFVPMEGDRYHISPEEAVKLIDENTIGVIGILGS 214
Query: 171 TGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGV 224
T G + I AL+D + +PLH+DAA GGF+ PF L DL +DF+L V
Sbjct: 215 TFDGSYEPIEALNDALETLNQRTGWQVPLHIDAASGGFIAPF---LDPDLR-WDFRLPWV 270
Query: 225 ESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAV 284
+SI HK G+ G II+R K+ L + GG + + +RPG V+A
Sbjct: 271 KSINTSGHKYGLVYPGVGWIIWRDKEELPEELIFHCNYLGGDLPNFALNFSRPGNQVVAQ 330
Query: 285 WALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNN-------------AWLVREPMLNIV 331
+ LG EGYR+I + + + + + +I +L AW +++ +L
Sbjct: 331 YYNFLRLGKEGYRKIQQTCRDTALYLSGKIAQLGPFELLTDGGDIPVFAWRLKDEVLANT 390
Query: 332 SFQTKNLRKVERELKRRGWGISAHRGYIRIVFMPHVTKEMV-------EEFLRDLREVL 383
+ L + +L+ RGW + A+R MP +++V E F RD+ ++L
Sbjct: 391 CY---TLYDMADKLRERGWLVPAYR-------MPKNREDLVVQRIVVKEGFSRDMADLL 439
>gb|AAF60394.1| (AC006701) contains similarity to Pfam family PF00282
(Pyridoxal-dependent decarboxylase conserved domain),
score=18, E=0.00021, N=1 [Caenorhabditis elegans]
Length = 402
Score = 109 bits (271), Expect = 4e-23
Identities = 66/210 (31%), Positives = 104/210 (49%), Gaps = 2/210 (0%)
Query: 145 DYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVI 204
D +V ++ I T I+ A G VD I L+ LA+ Y IPLHVD GGFV+
Sbjct: 56 DDRANVGAMKRAIGPRTCMIIASAPNHITGTVDPIEKLAKLAQRYHIPLHVDCTLGGFVL 115
Query: 205 PFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAG 264
PF + Y +P FDF+L GV SI+ D H+ G P +++R +L+ G
Sbjct: 116 PFMEYADYSVPAFDFRLPGVTSISADLHRYGQCPGRLSVLMYREPAFLRHQFFTNSEWPG 175
Query: 265 GKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVR 324
G T++G R G +V WA++ G +GY ++ +E +R A ++ L+ L
Sbjct: 176 GCYATPTMSGGRDGGAVATAWAMMLRKGRDGYINAAQRIIEATRQLAYRLQGLDGVSLRG 235
Query: 325 EPMLNIVSFQTK--NLRKVERELKRRGWGI 352
L +V+F T N+ + + ++GW +
Sbjct: 236 SADLCVVAFTTSEVNVYNLVDVMVQKGWHV 265
>gb|AAF63121.1|AC009526_6 (AC009526) Putative histidine decarboxylase [Arabidopsis thaliana]
Length = 482
Score = 108 bits (268), Expect = 9e-23
Identities = 94/342 (27%), Positives = 160/342 (46%), Gaps = 39/342 (11%)
Query: 56 NLGDP------GLHPGTRKIEEEVIEMLSDLLHLEKG--YGHIVSGGTEANILAVRAFRN 107
NLGDP G+H +R E V++ + L +E+ +G+I + GTE N+ + R
Sbjct: 130 NLGDPFIESNYGVH--SRPFEVGVLDWFARLWEIERDDYWGYITNCGTEGNLHGILVGRE 187
Query: 108 ISDAERPELIL--PKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTI--G 163
+ P+ IL + +H+S KA M ++ + +D D+ K+ N
Sbjct: 188 MF----PDGILYASRESHYSVFKAARMYRMECEKVDTLMSGEIDCDDLRKKLLANKDKPA 243
Query: 164 IVGI-AGTTGLGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFAKSLGYDLPDF 217
I+ + GTT G VDD+ + E G +H D A G ++PF K P
Sbjct: 244 ILNVNIGTTVKGAVDDLDLVIKTLEECGFSHDRFYIHCDGALFGLMMPFVKRA----PKV 299
Query: 218 DFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRP 277
F K + S+++ HK P+P G + R +++K +S YLA ATI G+R
Sbjct: 300 TFN-KPIGSVSVSGHKFVGCPMPCG-VQITRMEHIKVLSSNVEYLASR---DATIMGSRN 354
Query: 278 GASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKN 337
G + L +W + G++G+++ V+K + + + + +++ + MLN +S
Sbjct: 355 GHAPLFLWYTLNRKGYKGFQKEVQKCLRNAHYLKDRLREAGIS-----AMLNELSSTVVF 409
Query: 338 LRKVERELKRRGWGISAHRGYIRIVFMPHVTKEMVEEFLRDL 379
R + E RR W ++ +V MP VT E ++ FL+DL
Sbjct: 410 ERPKDEEFVRR-WQLACQGDIAHVVVMPSVTIEKLDNFLKDL 450
>gb|AAG22562.1|AF309077_2 (AF309077) glutamate decarboxylase [Listeria monocytogenes]
Length = 464
Score = 108 bits (267), Expect = 1e-22
Identities = 97/392 (24%), Positives = 179/392 (44%), Gaps = 46/392 (11%)
Query: 29 SSGKILGSMC-TMPHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHL---E 84
S+ + L + C T + A ++ + +++N D +P T ++E + +++DL H +
Sbjct: 53 SARQNLATFCQTYMEDEATKLMSETLEKNAIDKSEYPRTPELENRCVNIIADLWHAPKDQ 112
Query: 85 KGYGHIVSGGTEANILAVRA----FRNISD-------AERPELILPKSAHFSFIKAGEML 133
K G G ++A +L A +R ++ A++P L++ + K
Sbjct: 113 KFMGTSTIGSSDACMLGGMAMKFAWRKRAEKLGLDIYAKKPNLVISSGYQVCWEKFCVYW 172
Query: 134 GVKLVWAEL-KQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYG-- 190
+ + + K+ ++ V + + TIG+VGI G T G DDI AL++ EY
Sbjct: 173 DIDMRVVPMDKEHMQLNTDQVLDYVDEYTIGVVGILGITYTGRYDDIYALNEKLEEYNSK 232
Query: 191 ----IPLHVDAAFGGFVIPFAKSLGYDLPD--FDFKLKGVESITIDPHKMGMAPIPAGGI 244
+ +HVDAA GGF PF + PD +DF+LK V SI HK G+ G +
Sbjct: 233 TDYKVYIHVDAASGGFFTPFVE------PDIIWDFRLKNVISINTSGHKYGLVYPGIGWV 286
Query: 245 IFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAM 304
+++ + YL + GG++ I +R + ++ + GFEGYR I +K
Sbjct: 287 LWKDESYLPEELIFKVSYLGGEMPTMQINFSRSASHIIGQYYNFLRYGFEGYRTIHQKTS 346
Query: 305 ELSRWFAEEIKKLNNAWLVRE-PMLNIVSFQTKNLRKVE-------RELKRRGWGISAH- 355
+++++ A +++ + + L IV ++ K V L+ RGW + A+
Sbjct: 347 DVAQYLAHAVEQTGYFDIFNDGSHLPIVCYKLKTTANVNWTLYDLADRLQMRGWQVPAYP 406
Query: 356 --RGYIRIVFMPHVTK-----EMVEEFLRDLR 380
+ I+ +V + M EEF++D +
Sbjct: 407 LPKSLENIIIQRYVCRADLGFNMAEEFIQDFQ 438
>sp|Q42472|DCE2_ARATH GLUTAMATE DECARBOXYLASE 2 (GAD 2) >gi|1184960|gb|AAC33485.1|
(U46665) glutamate decarboxylase 2 [Arabidopsis
thaliana] >gi|1236619|gb|AAC31617.1| (U49937) glutamate
decarboxylase [Arabidopsis thaliana]
>gi|6227020|gb|AAF06056.1|AC009513_12 (AC009513)
Identical to gb|U46665 glutamate decarboxylase 2 (GAD 2)
Arabidopsis thaliana. ESTs gb|W43856, gb|N37724,
gb|Z34642 and gb|R90491 come from this gene
Length = 494
Score = 106 bits (261), Expect = 6e-22
Identities = 98/413 (23%), Positives = 184/413 (43%), Gaps = 40/413 (9%)
Query: 6 EKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGLHPG 65
E +P++ +++D+ +D + + T ++ I++N D +P
Sbjct: 33 ENSIPKDAAYQIIKDELMLDGN-PRLNLASFVTTWMEPECDKLIMDSINKNYVDMDEYPV 91
Query: 66 TRKIEEEVIEMLSDLLHL-----EKGYGHIVSGGTEANILAVRAFR----NISDAE---- 112
T +++ + +++ L + E G G +EA +LA AF+ N AE
Sbjct: 92 TTELQNRCVNIIARLFNAPLEESETAVGVGTVGSSEAIMLAGLAFKRKWQNKRKAEGKPY 151
Query: 113 -RPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKISD-NTIGIVGIAGT 170
+P ++ + + K V+L L + Y V D A++ D NTI + I G+
Sbjct: 152 DKPNIVTGANVQVCWEKFARYFEVELKEVNLSEGYYVMDPDKAAEMVDENTICVAAILGS 211
Query: 171 TGLGVVDDIPALSDLARE------YGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGV 224
T G +D+ L+DL + + P+HVDAA GGF+ PF Y ++DF+L V
Sbjct: 212 TLNGEFEDVKRLNDLLVKKNEETGWNTPIHVDAASGGFIAPFI----YPELEWDFRLPLV 267
Query: 225 ESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAV 284
+SI + HK G+ G +++R + L + G T+ ++ + ++A
Sbjct: 268 KSINVSGHKYGLVYAGIGWVVWRAAEDLPEELIFHINYLGADQPTFTLNFSKGSSQIIAQ 327
Query: 285 WALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-REPMLNIVSFQTK-----NL 338
+ + LGFEGY+ ++ +E E I+K +V ++ + +V+F K N
Sbjct: 328 YYQLIRLGFEGYKNVMENCIENMVVLKEGIEKTERFNIVSKDQGVPVVAFSLKDHSFHNE 387
Query: 339 RKVERELKRRGWGISA--------HRGYIRIVFMPHVTKEMVEEFLRDLREVL 383
++ L+R GW + A H +R+V ++ + E + D+ +VL
Sbjct: 388 FEISEMLRRFGWIVPAYTMPADAQHITVLRVVIREDFSRTLAERLVADISKVL 440
>sp|P28578|DCHS_KLEPL HISTIDINE DECARBOXYLASE (HDC) >gi|435602|gb|AAA25071.1| (M62746)
histidine decarboxylase [Klebsiella planticola]
Length = 378
Score = 105 bits (259), Expect = 1e-21
Identities = 86/293 (29%), Positives = 142/293 (48%), Gaps = 30/293 (10%)
Query: 46 IEVFARYIDRNLGDPGLHPG----TRKIEEEVIEMLSDLLHL--EKGYGHIVSGGTEANI 99
+E F R+ N GD G + + E+EV+E + L + E+ +G++ +GGTE N+
Sbjct: 39 LERFMRFSINNCGDWGEYCNYLLNSFDFEKEVMEYFAQLFKIPFEESWGYVTNGGTEGNM 98
Query: 100 LAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELK----QDYAVDVKDVEA 155
R I L K H+S K ++L +K E + DYA +K ++A
Sbjct: 99 FGCYLGREIFP--NGTLYYSKDTHYSVAKIVKLLRIKSTLVESQPNGEMDYADLIKKIKA 156
Query: 156 KISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFAKSL 210
+ I I GTT G +D+I + E GI LH DAA G ++PF
Sbjct: 157 DNEKHPIIFANI-GTTVRGAIDNIAIIQQSISELGIERKDYYLHADAALSGMILPFV--- 212
Query: 211 GYDLPD-FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQ 269
D P F+F G++SI + HKM +PIP GI+ +KK + ISV Y++
Sbjct: 213 --DNPQPFNFA-DGIDSIGVSGHKMIGSPIPC-GIVVAKKKNVDRISVEIDYIS---AHD 265
Query: 270 ATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLN-NAW 321
TI+G+R G + L +W I+ +E +R + +++ ++++ + + +AW
Sbjct: 266 KTISGSRNGHTPLMMWEAIRSHSWEEWRRRIERSLNMAQYAVDRFQSAGIDAW 318
>pir||D72734 probable histidine decarboxylase APE0412 - Aeropyrum pernix
(strain K1) >gi|5104052|dbj|BAA79368.1| (AP000059) 362aa
long hypothetical histidine decarboxylase [Aeropyrum
pernix]
Length = 362
Score = 105 bits (259), Expect = 1e-21
Identities = 78/264 (29%), Positives = 136/264 (50%), Gaps = 22/264 (8%)
Query: 33 ILGSMCTMPHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVS 92
ILG M P + A + RN GDPG P R+ E ++ + S ++ + G I S
Sbjct: 4 ILGGMVPRPPWKYRLLAAIHAHRNAGDPGADPLVRRAVERLVAVTSRVVFGVEKPGWITS 63
Query: 93 GGTEANILAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYA----V 148
G +E N+LA+ R + ++ ++H+S K+ L ++L +L + +
Sbjct: 64 GASEGNLLALYTLR---EEGYRRVVAFDTSHYSIKKSALTLSMEL---DLLPTHGGRPRL 117
Query: 149 DVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAK 208
D+ +E +I+ +++ +V GTT G+VD + ++ +A G +HVDAA+GG++ F
Sbjct: 118 DL--LERRITPDSV-VVATVGTTQEGLVDPVEEIAGVAESVGAAVHVDAAYGGYIARFLT 174
Query: 209 SLGYDLPDFDFKLK-GVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKV 267
F+L+ V ++ +D HK+ AP PAG I+ ++K L + +PY+ G+
Sbjct: 175 GKA------RFRLEPPVMTVVVDAHKIPEAPPPAGVILASKQKLLDNLWFESPYIPTGR- 227
Query: 268 WQATITGTRPGASVLAVWALIKHL 291
Q I GTRPG ++A W ++ L
Sbjct: 228 -QFGILGTRPGGPIVAAWRRVEDL 250
>pir||B40004 histidine decarboxylase (EC 4.1.1.22) - Klebsiella planticola
Length = 378
Score = 104 bits (256), Expect = 2e-21
Identities = 86/292 (29%), Positives = 142/292 (48%), Gaps = 28/292 (9%)
Query: 46 IEVFARYIDRNLGDPGLHPG----TRKIEEEVIEMLSDLLHL--EKGYGHIVSGGTEANI 99
+E F R+ N GD G + + E+EV+E + L + E+ +G++ +GGTE N+
Sbjct: 39 LERFMRFSINNCGDWGEYCNYLLNSFDFEKEVMEYFAQLFKIPFEESWGYVTNGGTEGNM 98
Query: 100 LAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKIS- 158
R I L K H+S K ++L +K E + + +D D+ KI
Sbjct: 99 FGCYLGREIFP--NGTLYYSKDTHYSVAKIVKLLRIKSTLVESQPNGEMDYADLIKKIKR 156
Query: 159 DNTIGIVGIA--GTTGLGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFAKSLG 211
DN + A GTT G +D+I + E GI LH DAA G ++PF
Sbjct: 157 DNEKHPIIFANIGTTVRGAIDNIAIIQQSISELGIERKDYYLHADAALSGMILPFV---- 212
Query: 212 YDLPD-FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQA 270
D P F+F G++SI + HKM +PIP GI+ +KK + ISV Y++
Sbjct: 213 -DNPQPFNFA-DGIDSIGVSGHKMIGSPIPC-GIVVAKKKNVDRISVEIDYIS---AHDK 266
Query: 271 TITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLN-NAW 321
TI+G+R G + L +W I+ +E +R + +++ ++++ + + +AW
Sbjct: 267 TISGSRNGHTPLMMWEAIRSHSWEEWRRRIERSLNMAQYAVDRFQSAGIDAW 318
>gb|AAG22560.1|AF309076_1 (AF309076) glutamate decarboxylase [Listeria monocytogenes]
Length = 462
Score = 102 bits (251), Expect = 9e-21
Identities = 91/337 (27%), Positives = 153/337 (45%), Gaps = 33/337 (9%)
Query: 5 PEKGLPREEVLNLLEDKTKVDLTFSSGKI---LGSMC-TMPHELAIEVFARYIDRNLGDP 60
PEK + +E V + + D G L + C T A ++ A +++N D
Sbjct: 24 PEKRMNKESVDPRIAYQLVKDQLIDEGSARQNLATFCQTYMEPEAEQIMAETMEKNAIDK 83
Query: 61 GLHPGTRKIEEEVIEMLSDLLHLEKG---YGHIVSGGTEANILA---------VRAFRNI 108
+P T K+E + ML+DL ++++ G G +EA +L A +N
Sbjct: 84 SEYPQTAKLESSCVNMLADLWNVDESEHYMGTSTVGSSEACMLGGMAMKFRWRSAALKNG 143
Query: 109 SD--AERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDY-AVDVKDVEAKISDNTIGIV 165
D A++P L++ + K ++L + +++ +++ + + + TIGIV
Sbjct: 144 LDIHAKKPNLVISSGYQVCWEKFCVYWDIELREVPMSEEHLSINTDIIMDYVDEYTIGIV 203
Query: 166 GIAGTTGLGVVDDIPALSDLAREYG------IPLHVDAAFGGFVIPFAKSLGYDLPDFDF 219
GI G T G DDI L+DL +Y + +HVD A G PF + G + +DF
Sbjct: 204 GILGITYTGKFDDIMTLNDLVEDYNNTHDNEVVIHVDGASGAMFTPFVEP-GLE---WDF 259
Query: 220 KLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGA 279
+L V SI HK G+ G I++R K+YL V GG + I +R +
Sbjct: 260 RLPNVVSINTSGHKYGLVYPGVGWILWRDKEYLPEELVFDVSYLGGHMPTMAINFSRSAS 319
Query: 280 SVLAVWALIKHLGFEGYREIVRK----AMELSRWFAE 312
++ + G+EGYR+I + A++LS+ AE
Sbjct: 320 QIIGQYYNFLRFGYEGYRQIHMRTRDGALQLSQAVAE 356
>sp|P54772|DCHS_LYCES HISTIDINE DECARBOXYLASE (HDC) (TOM92) >gi|481829|pir||S39554
histidine decarboxylase (EC 4.1.1.22) - tomato
>gi|416534|emb|CAA50719.1| (X71900) histidine
decarboxylase [Lycopersicon esculentum]
Length = 413
Score = 99.5 bits (244), Expect = 6e-20
Identities = 100/394 (25%), Positives = 171/394 (43%), Gaps = 37/394 (9%)
Query: 2 SKFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPG 61
+ F E + + L L ++ K + + +MC H + ++ N GDP
Sbjct: 9 TSFQELDMILTQYLETLSERKKYHIGYPI-----NMC-YEHHATLAPLLQFHLNNCGDPF 62
Query: 62 L-HPG---TRKIEEEVIEMLSDLLHLEKG--YGHIVSGGTEANILAVRAFRNISDAERPE 115
HP ++ E V++ + L +EK +G+I SGGTE N+ R
Sbjct: 63 TQHPTDFHSKDFEVAVLDWFAQLWEIEKDEYWGYITSGGTEGNLHGFWLGRR-ELLPNGY 121
Query: 116 LILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAK--ISDNTIGIVGI-AGTTG 172
L K +H+S KA M ++L + +D +D+++K ++ N I+ I GTT
Sbjct: 122 LYASKDSHYSIFKAARMYRMELQTINTLVNGEIDYEDLQSKLLVNKNKPAIININIGTTF 181
Query: 173 LGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESI 227
G +DD+ + G +H D A G ++PF K FK K + SI
Sbjct: 182 KGAIDDLDFVIQTLENCGYSNDNYYIHCDRALCGLILPFIKHA----KKITFK-KPIGSI 236
Query: 228 TIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWAL 287
+I HK P+ G I RR Y+ +S + Y+ ATI+G+R G + + +W
Sbjct: 237 SISGHKFLGCPMSCGVQITRRS-YVSTLSKIE-YINSA---DATISGSRNGFTPIFLWYC 291
Query: 288 IKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTKNLRKVERELKR 347
+ G ++ +E +R+ + + + + MLN S R + + R
Sbjct: 292 LSKKGHARLQQDSITCIENARYLKDRLLEAGIS-----VMLNDFSITVVFERPCDHKFIR 346
Query: 348 RGWGISAHRGYIRIVFMPHVTKEMVEEFLRDLRE 381
R W + RG +V MP +T+E ++ F +DL +
Sbjct: 347 R-WNLCCLRGMAHVVIMPGITRETIDSFFKDLMQ 379
>sp|P28577|DCHS_ENTAE HISTIDINE DECARBOXYLASE (HDC) >gi|95555|pir||A40004 histidine
decarboxylase (EC 4.1.1.22) - Enterobacter aerogenes
>gi|435594|gb|AAA24802.1| (M62745) histidine
decarboxylase [Enterobacter aerogenes]
Length = 378
Score = 96.7 bits (237), Expect = 4e-19
Identities = 86/273 (31%), Positives = 130/273 (47%), Gaps = 34/273 (12%)
Query: 46 IEVFARYIDRNLGDPGLHPG----TRKIEEEVIEMLSDLLHLE--KGYGHIVSGGTEANI 99
+E F R+ N GD G + + E+EV+E S + + + +G++ +GGTE+N+
Sbjct: 39 LERFLRFSINNCGDWGEYCNYLLNSFDFEKEVMEYFSGIFKIPFAESWGYVTNGGTESNM 98
Query: 100 LAVRAFRNISDAERPE--LILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKI 157
R + PE L K H+S K ++L +K E + D +D D+ KI
Sbjct: 99 FGCYLGRELF----PEGTLYYSKDTHYSVAKIVKLLRIKSQLVESQPDGEMDYDDLINKI 154
Query: 158 SDNTIG-----IVGIAGTTGLGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFA 207
T G I GTT G VD+I + GIP LH DAA G ++PF
Sbjct: 155 --RTSGERHPIIFANIGTTVRGAVDNIAEIQKRIAALGIPREDYYLHADAALSGMILPFV 212
Query: 208 KSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKV 267
+ D F F G++SI + HKM +PIP G I+ +K + ISV Y++
Sbjct: 213 E----DPQPFTFA-DGIDSIGVSGHKMIGSPIPCG-IVVAKKANVDRISVEIDYISAH-- 264
Query: 268 WQATITGTRPGASVLAVWALIK-HLGFEGYREI 299
TI+G+R G + L +WA ++ H E +R I
Sbjct: 265 -DKTISGSRNGHTPLMMWAAVRSHTDAEWHRRI 296
>pir||H82234 probable glutamate decarboxylase VC1149 [imported] - Vibrio
cholerae (group O1 strain N16961)
>gi|9655623|gb|AAF94308.1| (AE004195) glutamate
decarboxylase, putative [Vibrio cholerae]
Length = 548
Score = 92.1 bits (225), Expect = 1e-17
Identities = 78/288 (27%), Positives = 138/288 (47%), Gaps = 46/288 (15%)
Query: 84 EKGYGHIVSGGTEANILAVRAFRNIS----------------------DAERPELILPKS 121
E G SGGT ANI A+ RN + E +++ +
Sbjct: 163 EHSLGAFCSGGTIANITALWVARNNALKAEGDFPGVEKAGLFKAMRHYGHEGLAILVSER 222
Query: 122 AHFSFIKAGEMLGVK---LVWAELKQDYAVDVKDVEAKISD------NTIGIVGIAGTTG 172
H+S KA ++LG+ LV + + D+E KI++ +VG+AGTT
Sbjct: 223 GHYSLKKAADVLGIGQEGLVAVKTDAHNRICPHDLEQKITELKANKIKVFAVVGVAGTTE 282
Query: 173 LGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVE---SITI 229
G +D + ++ + + I H+DAA+GG + + G L GVE S+TI
Sbjct: 283 TGNIDPLRTIAQICQREQIHFHIDAAWGGATLMSNRYRGL--------LDGVELADSVTI 334
Query: 230 DPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYL--AGGK-VWQATITGTRPGASVLAVWA 286
D HK P+ AG ++F+ +++I A Y+ G K + T+ G+R G ++L V+A
Sbjct: 335 DAHKQLYIPMGAGMVLFKDPNAMRSIEHHAQYILRQGSKDLGSHTLEGSRSGMAML-VYA 393
Query: 287 LIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQ 334
+ + GY+ ++ +++E +R+FA+ I + LV +P L +++++
Sbjct: 394 SMHIISRPGYQLLIDQSIEKARYFADLIDAQTDFELVSQPELCLLTYR 441
>emb|CAA70530.1| (Y09356) pyridoxal-dependent histidine decarboxylase [Pseudomonas
fluorescens]
Length = 405
Score = 91.7 bits (224), Expect = 1e-17
Identities = 70/263 (26%), Positives = 127/263 (47%), Gaps = 22/263 (8%)
Query: 70 EEEVIEMLSDLLH--LEKGYGHIVSGGTEANILAVRAFRNISDAERPELILPKSAHFSFI 127
E++V+ ++L + LE +G++ +GGTE N+ R + L K H+S
Sbjct: 68 EKDVMTYFAELFNIALEDSWGYVTNGGTEGNMFGCYLGRELFPDGT--LYYSKDTHYSVA 125
Query: 128 KAGEMLGVKLVWAELKQDYAVDVKDVEAKISDNTIG---IVGIAGTTGLGVVDDIPALSD 184
K ++L +K E + +D D+ AKI+ + I GTT G +D+I +
Sbjct: 126 KIVKLLRIKCRAVESLPNGEIDYDDLMAKITADQERHPIIFANIGTTMRGALDNIVTIQQ 185
Query: 185 LAREYGIP-----LHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPI 239
++ GI LH DAA G ++PF D P G++SI + HKM +PI
Sbjct: 186 RLQQAGIARHDYYLHADAALSGMILPFV-----DHPQPFSFADGIDSICVSGHKMIGSPI 240
Query: 240 PAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREI 299
P G ++ +R + ISV Y+ + TI+G+R G + L +WA ++ + +R
Sbjct: 241 PCGIVVAKRNNVAR-ISVEVDYI---RAHDKTISGSRNGHTPLMMWAALRSYSWAEWRHR 296
Query: 300 VRKAMELSRWFAEEIKKLN-NAW 321
++ +++ +++ + + +AW
Sbjct: 297 IKHSLDTAQYAVDRFQASGIDAW 319
>sp|P80063|DCEA_ECOLI GLUTAMATE DECARBOXYLASE ALPHA (GAD-ALPHA) >gi|1073389|pir||S47737
GAD alpha protein - Escherichia coli
>gi|7428329|pir||S24234 glutamate decarboxylase (EC
4.1.1.15) alpha - Escherichia coli
>gi|146058|gb|AAA23833.1| (M84024) GAD alpha protein
[Escherichia coli] >gi|466654|gb|AAB18493.1| (U00039)
GAD alpha protein [Escherichia coli]
>gi|1789934|gb|AAC76542.1| (AE000428) glutamate
decarboxylase isozyme [Escherichia coli K12]
Length = 466
Score = 90.9 bits (222), Expect = 2e-17
Identities = 94/400 (23%), Positives = 173/400 (42%), Gaps = 70/400 (17%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMC-TMPHELAIEVFARYIDRNLGDPG 61
+FP + + ++ D+ +D ++ + L + C T E ++ I++N D
Sbjct: 31 RFPLHEMRDDVAFQIINDELYLD--GNARQNLATFCQTWDDENVHKLMDLSINKNWIDKE 88
Query: 62 LHPGTRKIEEEVIEMLSDLLHLE-----KGYGHIVSGGTEANILAVRA----FRNISDA- 111
+P + I+ + M++DL H + G G +EA +L A +R +A
Sbjct: 89 EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAA 148
Query: 112 ----ERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAK----------- 156
++P L+ V++ W + + + V+++++ +
Sbjct: 149 GKPTDKPNLVCGP--------------VQICWHKFARYWDVELREIPMRPGQLFMDPKRM 194
Query: 157 ---ISDNTIGIVGIAGTTGLGVVDDIPALSDLAREY------GIPLHVDAAFGGFVIPFA 207
+NTIG+V G T G + L D ++ I +H+DAA GGF+ PF
Sbjct: 195 IEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFV 254
Query: 208 KSLGYDLPD--FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGG 265
PD +DF+L V+SI+ HK G+AP+ G +I+R ++ L V GG
Sbjct: 255 A------PDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGG 308
Query: 266 KVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-- 323
++ I +RP V+A + LG EGY ++ + +++ + A+EI KL +
Sbjct: 309 QIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICT 368
Query: 324 --REPMLNIVSFQTKN-------LRKVERELKRRGWGISA 354
+ + V F+ K+ L + L+ RGW + A
Sbjct: 369 GRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA 408
>sp|P28302|DCEB_ECOLI GLUTAMATE DECARBOXYLASE BETA (GAD-BETA) >gi|482991|pir||B43332
glutamate decarboxylase (EC 4.1.1.15) beta - Escherichia
coli >gi|146060|gb|AAA23834.1| (M84025) glutamate
decarboxylase-beta [Escherichia coli]
>gi|1742450|dbj|BAA15163.1| (D90791) Glutamate
decarboxylase (EC 4.1.1.15) beta [Escherichia coli]
>gi|1787769|gb|AAC74566.1| (AE000246) glutamate
decarboxylase isozyme [Escherichia coli K12]
Length = 466
Score = 90.9 bits (222), Expect = 2e-17
Identities = 94/400 (23%), Positives = 173/400 (42%), Gaps = 70/400 (17%)
Query: 3 KFPEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMC-TMPHELAIEVFARYIDRNLGDPG 61
+FP + + ++ D+ +D ++ + L + C T E ++ I++N D
Sbjct: 31 RFPLHEMRDDVAFQIINDELYLD--GNARQNLATFCQTWDDENVHKLMDLSINKNWIDKE 88
Query: 62 LHPGTRKIEEEVIEMLSDLLHLE-----KGYGHIVSGGTEANILAVRA----FRNISDA- 111
+P + I+ + M++DL H + G G +EA +L A +R +A
Sbjct: 89 EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAA 148
Query: 112 ----ERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAK----------- 156
++P L+ V++ W + + + V+++++ +
Sbjct: 149 GKPTDKPNLVCGP--------------VQICWHKFARYWDVELREIPMRPGQLFMDPKRM 194
Query: 157 ---ISDNTIGIVGIAGTTGLGVVDDIPALSDLAREY------GIPLHVDAAFGGFVIPFA 207
+NTIG+V G T G + L D ++ I +H+DAA GGF+ PF
Sbjct: 195 IEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFV 254
Query: 208 KSLGYDLPD--FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGG 265
PD +DF+L V+SI+ HK G+AP+ G +I+R ++ L V GG
Sbjct: 255 A------PDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGG 308
Query: 266 KVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLV-- 323
++ I +RP V+A + LG EGY ++ + +++ + A+EI KL +
Sbjct: 309 QIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICT 368
Query: 324 --REPMLNIVSFQTKN-------LRKVERELKRRGWGISA 354
+ + V F+ K+ L + L+ RGW + A
Sbjct: 369 GRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA 408
>sp|Q9Z3R1|RHSB_RHIME L-2,4-DIAMINOBUTYRATE DECARBOXYLASE (DABA DECARBOXYLASE) (DABA-DC)
>gi|11352859|pir||T46815 diaminobutyrate decarboxylase
(EC 4.1.1.-) rhbB [imported] - Rhizobium meliloti
>gi|4151932|gb|AAD09413.1| (AF110737) RhbB
[Sinorhizobium meliloti]
Length = 495
Score = 89.7 bits (219), Expect = 5e-17
Identities = 87/321 (27%), Positives = 150/321 (46%), Gaps = 36/321 (11%)
Query: 44 LAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLE-KGYGHIVSGGTEANILAV 102
LA EV +++L P +EE V+ L+ L L G+ SGGT++N+ A+
Sbjct: 116 LAAEVLISATNQSLDSWDQSPFATLVEERVLACLTQLAELPASASGNFTSGGTQSNMTAL 175
Query: 103 --RAFRNISDAERPELILPKS-AHFSFIKAGEMLGVK---LVWAELKQDYAVDVKDVEAK 156
A R DA + ++L + AHFS K+ +LG ++ D + V ++A+
Sbjct: 176 YLAAVRCGPDARKAGVVLTSAHAHFSIRKSAAILGFAEDAVIAIAADADGRMSVPALKAE 235
Query: 157 I------SDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSL 210
+ + +V AGTT LG +D + ++DLA + +HVDAA+GG ++ F++
Sbjct: 236 LLRVAGEGRIPVAVVATAGTTDLGAIDPLVEIADLAAAQNVWMHVDAAYGGGLL-FSRHR 294
Query: 211 GYDLPDFDFKLKGVE---SITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYL----- 262
+L+G+E SIT+D HKM PI G ++ R + ++ A YL
Sbjct: 295 S--------RLEGLEHAHSITLDFHKMLFQPISCGVLLLRDRADFAPLASKADYLNPEDA 346
Query: 263 ----AGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLN 318
A V ++ T R A L + ++ +G +G ++ + ++ + AE +K
Sbjct: 347 VFADAPNLVERSMQTTRR--ADALKILMTMRAIGRDGLDALICQTLQNTHAAAEAVKTRE 404
Query: 319 NAWLVREPMLNIVSFQTKNLR 339
L P L+ V F+ + R
Sbjct: 405 YLSLAGPPSLSTVLFRYVSAR 425
>pir||S49218 histidine decarboxylase (EC 4.1.1.22) - Vibrio anguillarum
>gi|535939|emb|CAA83945.1| (Z33880) histidine
decarboxylase [Vibrio anguillarum]
Length = 386
Score = 88.2 bits (215), Expect = 1e-16
Identities = 93/358 (25%), Positives = 154/358 (42%), Gaps = 47/358 (13%)
Query: 46 IEVFARYIDRNLGD----PGLHPGTRKIEEEVIEMLSDLLHL--EKGYGHIVSGGTEANI 99
+E F ++ N GD + + E+EV+ S L + +G+I +GGTE N+
Sbjct: 39 LEKFMKFSINNCGDWREESNYKLNSFEFEKEVMRFFSQLFKIPYNDSWGYISNGGTEGNM 98
Query: 100 LAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGV------KLVWAELKQDYAVDVKDV 153
+ R I + + H+S K +L + L E+ DY V +
Sbjct: 99 FSCYLAREIFPTAY--IYYSEETHYSVDKIVRLLNIPARKIRSLPSGEI--DYQNLVDQI 154
Query: 154 EAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIP-----LHVDAAFGGFVIPFAK 208
+ N I I GTT G D+I + G+ +H DAA G ++PF +
Sbjct: 155 QKDKQKNPIIFANI-GTTMRGATDNIQRIQQDLASIGLERNDYYIHADAALSGMIMPFVE 213
Query: 209 SLG-YDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKV 267
Y D G++SI++ HKM +PIP GI+ ++ + ISV Y++
Sbjct: 214 QPHPYSFED------GIDSISVSGHKMIGSPIPC-GIVLAKRHMVDQISVEVDYISSR-- 264
Query: 268 WQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLN-NAWLVREP 326
TI+G+R G S L +W IK F ++ V + + ++ + + +++ NAW R
Sbjct: 265 -DQTISGSRNGHSALFMWTAIKSHSFVDWQGKVNQCLNMAEYTVQRFQEVGINAW--RNK 321
Query: 327 MLNIVSFQTKNLRKVERELKRRGWGISAHRGYIRIVFMPHV-----TKEMVEEFLRDL 379
N V F + V R+ G AH I+ MPH+ ++E+ + DL
Sbjct: 322 NSNTVVFPCPS-EPVWRKHSLANSGSVAH-----IITMPHLDGPDKLDPLIEDVIYDL 373
>dbj|BAA15157.1| (D90790) Glutamate decarboxylase (EC 4.1.1.15) beta [Escherichia
coli]
Length = 361
Score = 88.2 bits (215), Expect = 1e-16
Identities = 66/226 (29%), Positives = 107/226 (47%), Gaps = 25/226 (11%)
Query: 148 VDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREY------GIPLHVDAAFGG 201
+D K + +NTIG+V G T G + L D ++ I +H+DAA GG
Sbjct: 84 MDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG 143
Query: 202 FVIPFAKSLGYDLPD--FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLA 259
F+ PF PD +DF+L V+SI+ HK G+AP+ G +I+R ++ L V
Sbjct: 144 FLAPFVA------PDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFN 197
Query: 260 PYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNN 319
GG++ I +RP V+A + LG EGY ++ + +++ + A+EI KL
Sbjct: 198 VDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGP 257
Query: 320 AWLV----REPMLNIVSFQTKN-------LRKVERELKRRGWGISA 354
+ + + V F+ K+ L + L+ RGW + A
Sbjct: 258 YEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA 303
>gi|6323905 glutamate decarboxylase; Gad1p [Saccharomyces cerevisiae]
>gi|2494176|sp|Q04792|DCEH_YEAST PUTATIVE GLUTAMATE
DECARBOXYLASE (GAD) >gi|1078596|pir||S53072 glutamate
decarboxylase homolog YMR250w - yeast (Saccharomyces
cerevisiae) >gi|732928|emb|CAA88577.1| (Z48639) unknown
[Saccharomyces cerevisiae]
Length = 585
Score = 86.2 bits (210), Expect = 6e-16
Identities = 79/338 (23%), Positives = 144/338 (42%), Gaps = 29/338 (8%)
Query: 5 PEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPHELAIEVFARYIDRNLGDPGLHP 64
P+KGLP + L+ ++ +D + + T + A ++ + +NL D +P
Sbjct: 63 PKKGLPADLSYQLIHNELTLDGN-PHLNLASFVNTFTTDQARKLIDENLTKNLADNDEYP 121
Query: 65 GTRKIEEEVIEMLSDLLHL---EKGYGHIVSGGTEANILAVRAFRNI---------SDAE 112
++ + I ML+ L H E+ G +G +EA +L A + DA
Sbjct: 122 QLIELTQRCISMLAQLWHANPDEEPIGCATTGSSEAIMLGGLAMKKRWEHRMKNAGKDAS 181
Query: 113 RPELILPKSAHFSFIKAGEMLGV--KLVWAELKQDYAVDVKDVEAKISDNTIGIVGIAGT 170
+P +I+ + + K V +LV + + +D + + + +NTIG I GT
Sbjct: 182 KPNIIMSSACQVALEKFTRYFEVECRLVPVSHRSHHMLDPESLWDYVDENTIGCFVILGT 241
Query: 171 TGLGVVDDIPALSDLAREY----------GIPLHVDAAFGGFVIPFA----KSLGYDLPD 216
T G ++++ ++D+ + IP+H D A GGF+IPF Y +
Sbjct: 242 TYTGHLENVEKVADVLSQIEAKHPDWSNTDIPIHADGASGGFIIPFGFEKEHMKAYGMER 301
Query: 217 FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTR 276
+ F V S+ HK G+ G +++R + L GG + +R
Sbjct: 302 WGFNHPRVVSMNTSGHKFGLTTPGLGWVLWRDESLLADELRFKLKYLGGVEETFGLNFSR 361
Query: 277 PGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEI 314
PG V+ + LG GYR + ++ ++R F+ E+
Sbjct: 362 PGFQVVHQYFNFVSLGHSGYRTQFQNSLFVARAFSFEL 399
>emb|CAA44834.1| (X63123) glutamate decarboxylase [Escherichia coli]
Length = 284
Score = 86.2 bits (210), Expect = 6e-16
Identities = 65/226 (28%), Positives = 107/226 (46%), Gaps = 25/226 (11%)
Query: 148 VDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREY------GIPLHVDAAFGG 201
+D K + +NTIG+V G T G + L D ++ I +H+DAA GG
Sbjct: 42 MDPKRMIEACDENTIGVVPNFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGG 101
Query: 202 FVIPFAKSLGYDLPD--FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLA 259
F+ PF PD +DF+L V+SI+ HK G+AP+ G +I+R ++ + V
Sbjct: 102 FLAPFVA------PDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEAVPQELVFN 155
Query: 260 PYLAGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNN 319
GG++ I +RP V+A + LG EGY ++ + +++ + A+EI KL
Sbjct: 156 VDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGP 215
Query: 320 AWLV----REPMLNIVSFQTKN-------LRKVERELKRRGWGISA 354
+ + + V F+ K+ L + L+ RGW + A
Sbjct: 216 YEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA 261
>pir||T44576 L-2,4-diaminobutyrate decarboxylase (EC 4.1.1.-) [imported] -
Enterobacter aerogenes >gi|5917596|dbj|BAA84548.1|
(AB032468) L-2,4-diaminobutyrate decarboxylase
[Enterobacter aerogenes]
Length = 490
Score = 86.2 bits (210), Expect = 6e-16
Identities = 79/324 (24%), Positives = 146/324 (44%), Gaps = 37/324 (11%)
Query: 45 AIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKG-YGHIVSGGTEANILAVR 103
A EV ++++ P IE ++IE L + G G SGGT++N++ +
Sbjct: 99 AAEVLINATNQSMDSWDQSPSATIIEMKLIEWLRAQVGYPAGDAGVFTSGGTQSNLMGLM 158
Query: 104 AFRNI---------------SDAERPELILPKSAHFSFIKAGEMLGV---KLVWAELKQD 145
R+ D + +++ ++AHFS K ++G+ + + +
Sbjct: 159 LARDAFFARQGHSIQQDGIPGDIRQYKVLCSENAHFSVQKNMALMGLGYRSVTLVKTDEF 218
Query: 146 YAVDVKDVEAKIS------DNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAF 199
+DV D++AKI+ + + IV AGTT G +D + ++ +A E+ I LHVDAA+
Sbjct: 219 ARMDVTDLKAKIAQAQANGEQIMAIVATAGTTDAGAIDLLRDIAGIAAEHQIWLHVDAAW 278
Query: 200 GGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLA 259
GG ++ + D+ L+ V+S+T+D HK I G + + ++ + + A
Sbjct: 279 GGALL-----ISEQYRDYLDGLELVDSVTLDFHKQFFQTISCGAFLLKDARHYELMRYQA 333
Query: 260 PYL-------AGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWFAE 312
YL G + T L +W ++ LG + Y I+ + +++ AE
Sbjct: 334 AYLNSEFDEEHGVPNLVSKSLQTTRRFDALNLWMGLEALGKKQYASIIDHGVTMAKNVAE 393
Query: 313 EIKKLNNAWLVREPMLNIVSFQTK 336
+K LV +P L V F+++
Sbjct: 394 FVKSQPTLELVMQPQLASVLFRSR 417
>gb|AAF81243.1| (AF263012) amino acid decarboxylase-like protein [Streptomyces
griseus subsp. griseus]
Length = 525
Score = 83.9 bits (204), Expect = 3e-15
Identities = 75/265 (28%), Positives = 120/265 (44%), Gaps = 21/265 (7%)
Query: 64 PGTRKIEEEVIEMLSDLLH--LEKGYGHIVSGGTEANILAVRAFRNISDAERPELILPKS 121
P IEE L+ L+H I SGGTE+N++A+ R + ++ +
Sbjct: 134 PAAGVIEELTTAALARLIHPTAPAPDALITSGGTESNLVALLLARERAVPGTLRVVTGAN 193
Query: 122 AHFSFIKAGEMLGVKLVWAELKQDYAVD---VKDVEAKISDNTIGIVGIAGTTGLGVVDD 178
AH S +A MLG+ +D +D + D A ++ + +V AGTT G +D
Sbjct: 194 AHHSVHRAAWMLGLPAPVVVACRDGRIDPAALDDALASLAGAPLLVVATAGTTDEGRIDP 253
Query: 179 IPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAP 238
+P ++ +A + LHVDAA+GG ++ F++ L L L+ S+T D HK+G P
Sbjct: 254 LPEIARVAERHAARLHVDAAYGGPLL-FSERLAPLLAG----LEHAASVTFDLHKLGWQP 308
Query: 239 IPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATI---------TGTRPGASVLAVWALIK 289
+ AG + L +S+ A YL +A + T RP A +AV +
Sbjct: 309 VAAGVLAVADAGMLAPLSLRADYLNADDDTEAGLPDLLGRSIRTTRRPDALKMAV--TFR 366
Query: 290 HLGFEGYREIVRKAMELSRWFAEEI 314
LG G E+V + + F +
Sbjct: 367 ALGRRGLGELVEHCVRTAHEFGRAV 391
>gi|11120696 cysteine-sulfinate decarboxylase [Rattus norvegicus]
>gi|6685331|sp|Q64611|CSD_RAT CYSTEINE SULFINIC ACID
DECARBOXYLASE (SULFINOALANINE DECARBOXYLASE)
(CYSTEINE-SULFINATE DECARBOXYLASE)
>gi|7436482|pir||S71489 sulfoalanine decarboxylase (EC
4.1.1.29) - rat >gi|1263164|emb|CAA63868.1| (X94152)
cysteine sulfinate decarboxylase [Rattus norvegicus]
>gi|5830496|emb|CAB54561.1| (AJ132661)
cysteine-sulfinate decarboxylase [Rattus norvegicus]
Length = 493
Score = 83.5 bits (203), Expect = 4e-15
Identities = 99/378 (26%), Positives = 163/378 (42%), Gaps = 64/378 (16%)
Query: 41 PHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANIL 100
PH LA + ++ + + P +EEEV++ L L+ G G GG+ +N+
Sbjct: 99 PHALAGRIITESLNTSQYTYEIAPVFVLMEEEVLKKLRALVGWNTGDGVFCPGGSISNMY 158
Query: 101 AVRA--FRNISDAER------PELIL--PKSAHFSFIKAGEMLG-----VKLVWAE---- 141
A+ F+ D ++ P L L K H+S K LG V++V A+
Sbjct: 159 AINLARFQRYPDCKQRGLRALPPLALFTSKECHYSITKGAAFLGLGTDSVRVVKADERGK 218
Query: 142 -LKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFG 200
+ +D + EA+ S + + +GTT LG D + A++D+ + +G+ LHVDAA+G
Sbjct: 219 MIPEDLERQISLAEAEGSVPFL-VSATSGTTVLGAFDPLDAIADVCQRHGLWLHVDAAWG 277
Query: 201 GFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYL--KAISVL 258
G V+ +++ + L ++ +S+ +PHK+ A + ++ R L +
Sbjct: 278 GSVL-LSRTHRHLLDG----IQRADSVAWNPHKLLAAGLQCSALLLRDTSNLLKRCHGSQ 332
Query: 259 APYL------------AGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMEL 306
A YL G KV Q G R L +W + K G +G + +A L
Sbjct: 333 ASYLFQQDKFYNVALDTGDKVVQC---GRR--VDCLKLWLMWKAQGGQGLEWRIDQAFAL 387
Query: 307 SRWFAEEIKKLNNAWLVREPMLNIVSF---------------QTKNLRKVERELKRRGWG 351
+R+ EEIKK LV EP V F ++ L +V LK R
Sbjct: 388 TRYLVEEIKKREGFELVMEPEFVNVCFWFVPPSLRGKKESPDYSQRLSQVAPVLKER--- 444
Query: 352 ISAHRGYIRIVFMPHVTK 369
+G + I + PH T+
Sbjct: 445 -MVKKGTMMIGYQPHGTR 461
>gb|AAG12476.1|AC037197_1 (AC037197) Putatitve histidine decarboxylase [Oryza sativa]
Length = 424
Score = 83.5 bits (203), Expect = 4e-15
Identities = 72/254 (28%), Positives = 119/254 (46%), Gaps = 32/254 (12%)
Query: 49 FARYIDRNLGDP------GLHPGTRKIEEEVIEMLSDLLHLEKG--YGHIVSGGTEANIL 100
F + N GDP +H +R+ E V+ ++ +++ +G+I SGGTE N+
Sbjct: 100 FLNFSLNNAGDPFAKVNNSVH--SRQFEVAVLNWFANFWDVQRDQFWGYITSGGTEGNLY 157
Query: 101 AVRAFRNISDAERPELILPKS--AHFSFIKAGEMLGVKLVWAELKQDYAVDVKDVEAKIS 158
+ R + P+ IL S +H+S KA +M VK + ++ D+++K+
Sbjct: 158 GLLVGRELF----PDGILYASNDSHYSVFKAAKMYRVKCIRIATTVSGEMNYADLKSKLQ 213
Query: 159 DNTIG---IVGIAGTTGLGVVDDIPALSDLAREYGIP----LHVDAAFGGFVIPFAKSLG 211
NT I GTT G VDDI + + G +H D+A G + PF K
Sbjct: 214 HNTNSPAIINANIGTTFKGAVDDIDQIISTLEKCGFQNRYYIHCDSALSGMMTPFMK--- 270
Query: 212 YDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQAT 271
P FK K + SI++ HK P+P G++ R ++ + +S Y+A +T
Sbjct: 271 -QAPKVSFK-KPIGSISVSGHKFLGCPMPC-GVVITRLEHAEVLSTDIEYIASR---DST 324
Query: 272 ITGTRPGASVLAVW 285
ITG+R G + + +W
Sbjct: 325 ITGSRNGHAPIFLW 338
>pir||S55689 sulfoalanine decarboxylase (EC 4.1.1.29) - rat
>gi|847653|gb|AAC42063.1| (M64755) cysteine sulfinic
acid decarboxylase [Rattus norvegicus]
>gi|1096956|prf||2113203A Cys sulfinic acid
decarboxylase [Rattus norvegicus]
Length = 478
Score = 82.7 bits (201), Expect = 7e-15
Identities = 89/327 (27%), Positives = 147/327 (44%), Gaps = 45/327 (13%)
Query: 41 PHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANIL 100
PH LA + ++ + + P +EEEV++ L L+ G G GG+ +N+
Sbjct: 99 PHALAGRIITESLNTSQYTYEIAPVFVLMEEEVLKKLRALVGWNTGDGVFCPGGSISNMY 158
Query: 101 AVRA--FRNISDAERPEL-ILP-------KSAHFSFIKAGEMLG-----VKLVWAE---- 141
A+ F+ D ++ L +LP K H+S K LG V++V A+
Sbjct: 159 AINLARFQRYPDCKQRGLRVLPPLALFTSKECHYSITKGAAFLGLGTDSVRVVKADERGK 218
Query: 142 -LKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFG 200
+ +D + EA+ S + + +GTT LG D + A++D+ + +G+ LHVDAA+G
Sbjct: 219 MIPEDLERQISLAEAEGSVPFL-VSATSGTTVLGAFDPLDAIADVCQRHGLWLHVDAAWG 277
Query: 201 GFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYL--KAISVL 258
G V+ +++ + L ++ +S+ +PHK+ A + ++ R L +
Sbjct: 278 GSVL-LSRTHRHLLDG----IQRADSVAWNPHKLLAAGLQCSALLLRDTSNLLKRCHGSQ 332
Query: 259 APYL------------AGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMEL 306
A YL G KV Q G R L +W + K G +G + +A L
Sbjct: 333 ASYLFQQDKFYNVALDTGDKVVQC---GRR--VDCLKLWLMWKAQGGQGLEWRIDQAFAL 387
Query: 307 SRWFAEEIKKLNNAWLVREPMLNIVSF 333
+R+ EEIKK LV EP V F
Sbjct: 388 TRYLVEEIKKREGFELVMEPEFVNVCF 414
>dbj|BAB06342.1| (AP001516) L-2,4-diaminobutyrate decarboxylase [Bacillus
halodurans]
Length = 508
Score = 79.2 bits (192), Expect = 7e-14
Identities = 78/324 (24%), Positives = 142/324 (43%), Gaps = 39/324 (12%)
Query: 44 LAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLL-HLEKGYGHIVSGGTEANILAV 102
L EV +++++ P +E E++ + + + + G G SGGT++N + +
Sbjct: 105 LVAEVVINALNQSMDSWDQSPSATYVETELLRFFTRSIGYSDLGDGVFTSGGTQSNYMGM 164
Query: 103 RAFRNIS----------------DAERPELILPKSAHFSFIKAGEMLGV---KLVWAELK 143
RN DA + ++ + AHFS K+ LG+ +V
Sbjct: 165 LLARNKICESAFATNVQEEGLPVDARKLRILCSQEAHFSVQKSAAQLGLGANAVVQVATN 224
Query: 144 QDYAVDVKDVEAKISDNTIG------IVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDA 197
+ + V + I++ + IV AGTT G +D + +++LARE + LHVDA
Sbjct: 225 ERQQLCVNETRQTINELRLKGLIPFMIVATAGTTDFGSIDKLREIAELAREQELWLHVDA 284
Query: 198 AFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISV 257
A+GG ++ F++ G+ L L +SITID HK I G + + + I
Sbjct: 285 AYGGALL-FSRQYGHLLNG----LHLADSITIDFHKFYYQSISCGAFFVKDRHEFERIRF 339
Query: 258 LAPYLAGGKVWQATITG-------TRPGASVLAVWALIKHLGFEGYREIVRKAMELSRWF 310
A YL + +A I T L + K +G E + +++ ++L++
Sbjct: 340 HADYLNPEEDKEADIINLVEKSVQTTKRFDALKLLVTFKWMGTEQFGQMIDYTIQLAKET 399
Query: 311 AEEIKKLNNAWLVREPMLNIVSFQ 334
E +++ ++ +P LN V F+
Sbjct: 400 GELLRRYGFD-VLNDPELNAVVFR 422
>emb|CAB71551.1| (AJ270937) aromatic amino acid decarboxylase [Sorangium cellulosum]
Length = 512
Score = 79.2 bits (192), Expect = 7e-14
Identities = 76/281 (27%), Positives = 126/281 (44%), Gaps = 47/281 (16%)
Query: 64 PGTRKIEEEVIEMLSDLLHLEKG-YGHIVSGGTEANILAVRA----------FRNISDAE 112
P ++EE V+ L +L L+ G +G I+ + A+++A+ A R ++
Sbjct: 120 PAATELEELVLSWLRQMLDLDAGLHGAIMDTASTASMVAIAAARDSAEPTIRLRGMAGQR 179
Query: 113 RPELILPKSAHFSFIKAGEMLGV--------------KLVWAELKQDYAVDVKDVEAKIS 158
R L + AH S KA LG+ ++V L+ A V+D+ A +
Sbjct: 180 RMRLYASEQAHSSIEKAAITLGIGQEGVRKIPTDPAFRMVPEALR---AAVVEDLGAGL- 235
Query: 159 DNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGF--VIPFAKSLGYDLPD 216
+ GTT VD IPA+ + RE+G+ LHVDAA+ G ++P + D
Sbjct: 236 -RPFCVAATVGTTSTTSVDPIPAIVSVCREHGLWLHVDAAYAGMAAIVP-------EHRD 287
Query: 217 FDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLK-AISVLAPYL-AGGKVWQATITG 274
+G +S+ ++PHK P+ + R LK A S++ YL G V G
Sbjct: 288 VLAGCEGADSLVVNPHKWLFTPMDCSVLYVRDADRLKRAFSLVPEYLRTEGDVTNYMDWG 347
Query: 275 TRPGA--SVLAVWALIKHLGFEG----YREIVRKAMELSRW 309
+ G L +W ++++ G EG RE +R +L++W
Sbjct: 348 IQLGRRFRALKLWMIVRYFGHEGLAARIREHLRLGQQLAQW 388
>gb|AAF73187.1|AF149833_1 (AF149833) glutamic acid decarboxylase isoform 67 [Carassius
auratus]
Length = 587
Score = 78.4 bits (190), Expect = 1e-13
Identities = 77/270 (28%), Positives = 119/270 (43%), Gaps = 38/270 (14%)
Query: 86 GYGHIVSGGTEANILAVRAFR----------NISDAERPELILPKSAHFSFIKAGEMLGV 135
G G GG +N+ +V R +S A R L + +H+S KAG +LG
Sbjct: 237 GDGLFSPGGAISNMYSVMVARYKYFPEVKTKGMSAAPRLVLFTSEHSHYSIKKAGAVLGF 296
Query: 136 ---KLVWAELKQDYAVDVKDVEAKISDNT------IGIVGIAGTTGLGVVDDIPALSDLA 186
++ + + V D+EAKI D + + AGTT G D I ++D+
Sbjct: 297 GRENVILLKTDERGRVIPADLEAKIIDAKQKGYVPLFVNATAGTTVYGAFDPINDIADIC 356
Query: 187 REYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVE---SITIDPHKMGMAPIPAGG 243
+Y + LHVD A+GG ++ K KL G+E S+T +PHKM AP+
Sbjct: 357 EKYNLWLHVDGAWGGGLLMSRK--------HRHKLSGIERANSVTWNPHKMMGAPLQCSA 408
Query: 244 IIFRRKKYLKAI-SVLAPYL-AGGKVWQATI-TGTRP-----GASVLAVWALIKHLGFEG 295
I+ R K L+ S+ A YL K + T TG + + W + K G G
Sbjct: 409 ILVREKGILQGCNSMCAGYLFQPDKQYDVTYDTGDKAIQCGRHVDIFKFWLMWKAKGTIG 468
Query: 296 YREIVRKAMELSRWFAEEIKKLNNAWLVRE 325
+ + + + +ELS + +IK +V E
Sbjct: 469 FEQHIDRCLELSEYLYNKIKNREGYEMVFE 498
>gb|AAG33932.1|AF045595_1 (AF045595) glutamic acid decarboxylase isoform 67; GAD67; glutamate
decarboxylase [Carassius auratus]
Length = 587
Score = 78.0 bits (189), Expect = 2e-13
Identities = 77/270 (28%), Positives = 119/270 (43%), Gaps = 38/270 (14%)
Query: 86 GYGHIVSGGTEANILAVRAFR----------NISDAERPELILPKSAHFSFIKAGEMLGV 135
G G GG +N+ +V R +S A R L + +H+S KAG +LG
Sbjct: 237 GDGLFSPGGAISNMYSVMVARYKYFPEVKTKGMSVAPRLVLFTSEHSHYSIKKAGAVLGF 296
Query: 136 ---KLVWAELKQDYAVDVKDVEAKISDNT------IGIVGIAGTTGLGVVDDIPALSDLA 186
++ + + V D+EAKI D + + AGTT G D I ++D+
Sbjct: 297 GKENVILLKTDERGRVIPADLEAKIIDAKQKGYVPLFVNATAGTTVYGAFDPINDIADIC 356
Query: 187 REYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVE---SITIDPHKMGMAPIPAGG 243
+Y + LHVD A+GG ++ K KL G+E S+T +PHKM AP+
Sbjct: 357 EKYNLWLHVDGAWGGGLLMSRK--------HRHKLSGIERANSVTWNPHKMMGAPLQCSA 408
Query: 244 IIFRRKKYLKAI-SVLAPYL-AGGKVWQATI-TGTRP-----GASVLAVWALIKHLGFEG 295
I+ R K L+ S+ A YL K + T TG + + W + K G G
Sbjct: 409 ILVREKGILQGCNSMCAGYLFQPDKQYDVTYDTGDKAIQCGRHVDIFKFWLMWKAKGTIG 468
Query: 296 YREIVRKAMELSRWFAEEIKKLNNAWLVRE 325
+ + + + +ELS + +IK +V E
Sbjct: 469 FEQHIDRCLELSEYLYNKIKNREGYEMVFE 498
>gb|AAG20873.1| (AE005155) L-2,4-diaminobutyrate decarboxylase; Bdb [Halobacterium
sp. NRC-1]
Length = 486
Score = 77.6 bits (188), Expect = 2e-13
Identities = 88/369 (23%), Positives = 157/369 (41%), Gaps = 38/369 (10%)
Query: 5 PEKGLPREEVLNLLEDKTKVDLTFSSGKILGSMCTMPH---ELAIEVFARYIDRNLGDPG 61
P +G P E+VL + + + S + + P LA E +++L
Sbjct: 38 PAEGHPLEDVLAAVREDVLANAVHPSDETCVAHLQCPPMVPALAAEALLTASNQSLDSFD 97
Query: 62 LHPGTRKIEEEVIEMLSDLLHLEKGY-GHIVSGGTEANILAVRAFRNI------------ 108
P +EE +I L+ L L G + GGTE+N A+ R+
Sbjct: 98 QAPAATVVEERLIADLTALYELGPAADGVVTGGGTESNHQALLLARDDYVETVFGQSVRE 157
Query: 109 ----SDAERPELILPKSAHFSFIKAGEMLGV---KLVWAELKQDYAVD---VKDVEAKIS 158
A+ ++ AHF+ ++ +LG+ +V + +D +++ +++
Sbjct: 158 HGLPPAAQDLRILCSADAHFTAAQSAALLGLGEDAVVTIPTDGRHRMDATALRETVERLN 217
Query: 159 D---NTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFAKSLGYDLP 215
D + +VG AGTT G +D + A++D+A + + +HVDAAFGG + + L
Sbjct: 218 DAGKHPFAVVGTAGTTDFGSIDPLSAVADVAATHDLWVHVDAAFGG-ALAVSDQHSERLS 276
Query: 216 DFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYL--AGGKVWQATIT 273
D +S+ +D HK+ PI G ++ + + +S A YL AG V
Sbjct: 277 GID----RADSVAVDFHKLFFQPIACGALLVADGESFELMSRNAAYLNPAGDAVPNLVAK 332
Query: 274 GTRPGA--SVLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIV 331
TR L + + LG +G +V + + L+ A ++ L EP LN V
Sbjct: 333 STRTTRRFDALKPYVAFRTLGRDGLGALVDRTLALADDVAGLLRADPAFELACEPTLNAV 392
Query: 332 SFQTKNLRK 340
+F+ + +R+
Sbjct: 393 TFRYRPVRE 401
>sp|Q9Y600|CSD_HUMAN CYSTEINE SULFINIC ACID DECARBOXYLASE (SULFINOALANINE DECARBOXYLASE)
(CYSTEINE-SULFINATE DECARBOXYLASE)
>gi|4894560|gb|AAD32545.1|AF116547_1 (AF116547) cysteine
sulfinic acid decarboxylase-related protein 3 [Homo
sapiens]
Length = 493
Score = 76.9 bits (186), Expect = 4e-13
Identities = 97/378 (25%), Positives = 156/378 (40%), Gaps = 64/378 (16%)
Query: 41 PHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANIL 100
PH LA + ++ + + P +EEEV+ L L+ G G GG+ +N+
Sbjct: 99 PHALAGRIITESLNTSQYTYEIAPVFVLMEEEVLRKLRALVGWSSGDGIFCPGGSISNMY 158
Query: 101 AVRA--FRNISDAER------PELIL--PKSAHFSFIKAGEMLG-----VKLVWAE---- 141
AV ++ D ++ P L L K H+S K LG V++V A+
Sbjct: 159 AVNLARYQRYPDCKQRGLRTLPPLALFTSKECHYSIQKGAAFLGLGTDSVRVVKADERGK 218
Query: 142 -LKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFG 200
+ +D + EA+ + + + +GTT LG D + A++D+ + +G+ LHVDAA+G
Sbjct: 219 MVPEDLERQIGMAEAEGAVPFL-VSATSGTTVLGAFDPLGAIADVCQRHGLWLHVDAAWG 277
Query: 201 GFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYL--KAISVL 258
G V+ L ++ +S+ +PHK+ A + ++ + L +
Sbjct: 278 GSVL-----LSQTHRHLLDGIQRADSVAWNPHKLLAAGLQCSALLLQDTSNLLKRCHGSQ 332
Query: 259 APYL------------AGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMEL 306
A YL G KV Q G R L +W + K G +G + +A L
Sbjct: 333 ASYLFQQDKFYDVALDTGDKVVQC---GRR--VDCLKLWLMWKAQGDQGLERRIDQAFVL 387
Query: 307 SRWFAEEIKKLNNAWLVREPMLNIVSF---------------QTKNLRKVERELKRRGWG 351
+R+ EE+KK LV EP V F + L KV LK R
Sbjct: 388 ARYLVEEMKKREGFELVMEPEFVNVCFWFVPPSLRGKQESPDYHERLSKVAPVLKER--- 444
Query: 352 ISAHRGYIRIVFMPHVTK 369
G + I + PH T+
Sbjct: 445 -MVKEGSMMIGYQPHGTR 461
>gb|AAD32546.1|AF116548_1 (AF116548) cysteine sulfinic acid decarboxylase-related protein 4
[Homo sapiens]
Length = 493
Score = 76.5 bits (185), Expect = 5e-13
Identities = 97/378 (25%), Positives = 156/378 (40%), Gaps = 64/378 (16%)
Query: 41 PHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGYGHIVSGGTEANIL 100
PH LA + ++ + + P +EEEV+ L L+ G G GG+ +N+
Sbjct: 99 PHALAGRIITESLNTSQYTYEIAPVFVLMEEEVLRKLRALVGWSSGDGIFCPGGSISNMY 158
Query: 101 AVRA--FRNISDAER------PELIL--PKSAHFSFIKAGEMLG-----VKLVWAE---- 141
AV ++ D ++ P L L K H+S K LG V++V A+
Sbjct: 159 AVNLARYQRYPDCKQRGLRTLPPLALFTSKECHYSIQKGAAFLGLGTDSVRVVKADERGK 218
Query: 142 -LKQDYAVDVKDVEAKISDNTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFG 200
+ +D + EA+ + + + +GTT LG D + A++D+ + +G+ LHVDAA+G
Sbjct: 219 MVPEDLERQIGMAEAEGAVPFL-VSATSGTTVLGAFDPLEAIADVCQRHGLWLHVDAAWG 277
Query: 201 GFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYL--KAISVL 258
G V+ L ++ +S+ +PHK+ A + ++ + L +
Sbjct: 278 GSVL-----LSQTHRHLLDGIQRADSVAWNPHKLLAAGLQCSALLLQDTSNLLKRCHGSQ 332
Query: 259 APYL------------AGGKVWQATITGTRPGASVLAVWALIKHLGFEGYREIVRKAMEL 306
A YL G KV Q G R L +W + K G +G + +A L
Sbjct: 333 ASYLFQQDKFYDVALDTGDKVVQC---GRR--VDCLKLWLMWKAQGDQGPERRIDQAFVL 387
Query: 307 SRWFAEEIKKLNNAWLVREPMLNIVSF---------------QTKNLRKVERELKRRGWG 351
+R+ EE+KK LV EP V F + L KV LK R
Sbjct: 388 ARYLVEEMKKREGFELVMEPEFVNVCFWFVPPSLRGKQESPDYHERLSKVAPVLKER--- 444
Query: 352 ISAHRGYIRIVFMPHVTK 369
G + I + PH T+
Sbjct: 445 -MVKEGSMMIGYQPHGTR 461
>emb|CAB87219.1| (AL163641) putative pyridoxal-dependent decarboxylase [Streptomyces
coelicolor A3(2)]
Length = 480
Score = 73.0 bits (176), Expect = 6e-12
Identities = 58/242 (23%), Positives = 113/242 (45%), Gaps = 30/242 (12%)
Query: 88 GHIVSGGTEANILAVRAFRNISDAER---PELILPKSAHFSFIKAGEMLGV---KLVWAE 141
G SGGT++N+ A+ R + AE + +++HFS K+ ++LG+ +V
Sbjct: 141 GVFTSGGTQSNLQALLLAREEAKAEDFADLRIFASEASHFSVRKSAKLLGLGPDAVVSIP 200
Query: 142 LKQDYAVDV----KDVEAKISDNTI--GIVGIAGTTGLGVVDDIPALSDLAREYGIPLHV 195
+ +D + +++E D + +V GTT G +D +P ++ L +YG+ +HV
Sbjct: 201 VDRDKRMQTVALARELERCARDGLVPMAVVATGGTTDFGSIDPLPEIAGLCEQYGVWMHV 260
Query: 196 DAAFGGFVIPFAKSLGYDLPDFDFKLKGVE---SITIDPHKMGMAPIPAGGIIFRRKKYL 252
DAA+G ++ K + ++ G+E S+T+D HK P+ + ++ R L
Sbjct: 261 DAAYGCGLLASLK--------YRDRITGIERADSVTVDYHKSFFQPVSSSAVLVRDAATL 312
Query: 253 KAISVLAPYLAGGKVWQATITG-------TRPGASVLAVWALIKHLGFEGYREIVRKAME 305
+ + A YL ++ Q I T L +W ++ +G +G + + +
Sbjct: 313 RHATYHAEYLNPRRMVQERIPNQVDKSLQTTRRFDALKLWMTLRVMGADGIGVLFDEVCD 372
Query: 306 LS 307
L+
Sbjct: 373 LA 374
>pir||T35762 probable amino acid decarboxylase - Streptomyces coelicolor
>gi|5689910|emb|CAB52073.1| (AL109732) putative amino
acid decarboxylase [Streptomyces coelicolor A3(2)]
Length = 452
Score = 71.4 bits (172), Expect = 2e-11
Identities = 70/254 (27%), Positives = 112/254 (43%), Gaps = 15/254 (5%)
Query: 90 IVSGGTEANILAVRAFRNISDAERPELILPKSAHFSFIKAGEMLGVKLVWAELKQDYAVD 149
+ +GGTE+N LAV R A L+ AH S +A +LG+ +D
Sbjct: 143 VTTGGTESNQLAVLLARERHGAGL-RLVHGAGAHHSLPRAAWLLGLPEPVVVPAPAGTLD 201
Query: 150 VKDVEAKISD--NTIGIVGIAGTTGLGVVDDIPALSDLAREYGIPLHVDAAFGGFVIPFA 207
++ ++ + AGTT G++D +P ++D +G LHVDAA+G ++ F+
Sbjct: 202 PAALDEALTQVPGPHLVAATAGTTDAGLIDPLPEIADRCAAHGARLHVDAAYGAGLL-FS 260
Query: 208 KSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKV 267
+ L L+ +++ +D HK+G PIPAG + L A+ A YL
Sbjct: 261 ERHRARLAG----LEAADTVALDLHKLGWQPIPAGLLTVTDADDLGALHHRADYLNADDD 316
Query: 268 WQA---TITGTRPGAS----VLAVWALIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNA 320
A + G P S VL +K LG G +V L+R FA+ ++
Sbjct: 317 TDAGLPDLLGRSPRTSRRPDVLKTAVTLKTLGRAGLGALVDAVCSLAREFADLVETHPGF 376
Query: 321 WLVREPMLNIVSFQ 334
L P ++ V F+
Sbjct: 377 ELHAPPTISTVLFR 390
>sp|P71362|DDC_HAEIN L-2,4-DIAMINOBUTYRATE DECARBOXYLASE (DABA DECARBOXYLASE) (DABA-DC)
>gi|7467562|pir||JC5880 L-2,4-diaminobutyrate
decarboxylase (EC 4.1.1.-) [validated] - Haemophilus
influenzae >gi|1573971|gb|AAC22607.1| (U32776)
L-2,4-diaminobutyrate decarboxylase [Haemophilus
influenzae Rd]
Length = 511
Score = 69.9 bits (168), Expect = 5e-11
Identities = 78/310 (25%), Positives = 130/310 (41%), Gaps = 45/310 (14%)
Query: 39 TMPHELAIEVFARYIDRNLGDPGLHPGTRKIEEEVIEMLSDLLHLEKGY-GHIVSGGTEA 97
TM EV ++++ P +EE +I L +G G SGGT++
Sbjct: 98 TMVTSQIAEVLINATNQSMDSWDQSPAGSIMEEHLINWLRQKAGYGEGTSGVFTSGGTQS 157
Query: 98 NILAVRAFRNISDA-----------------------ERPELILPKSAHFSFIKAGEMLG 134
N++ V R+ + A ++ ++I ++AHFS K M+G
Sbjct: 158 NLMGVLLARDWAIANHWKNEDGSEWSVQRDGIPAEAMQKVKVICSENAHFSVQKNMAMMG 217
Query: 135 V---KLVWAELKQDYAVDV---KDVEAKISDN---TIGIVGIAGTTGLGVVDDIPALSDL 185
+ +V + +D+ K A++ + T IV AGTT G +DD+ A+ L
Sbjct: 218 MGFQSVVTVPSNANAQMDLIALKQTLAQLKADGKITACIVATAGTTDAGAIDDLKAIRKL 277
Query: 186 AREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVESITIDPHKMGMAPIPAGGII 245
A EY LHVDAA+GG ++ +K Y L + +SIT+D HK I G +
Sbjct: 278 ADEYQAWLHVDAAWGGALL-LSKDYRYFLDGIEL----TDSITLDFHKHFFQTISCGAFL 332
Query: 246 FRRKKYLKAISVLAPYL-------AGGKVWQATITGTRPGASVLAVWALIKHLGFEGYRE 298
+ + + I A YL G A T L +W ++ LG + Y
Sbjct: 333 LKDPENYRFIDYKADYLNSEYDEAHGVPNLVAKSLQTTRRFDALKLWFTLEALGEDLYAS 392
Query: 299 IVRKAMELSR 308
++ ++L++
Sbjct: 393 MIDHGVKLTK 402
>pir||T26636 hypothetical protein Y37D8A.23 - Caenorhabditis elegans
>gi|3925209|emb|CAA21537.1| (AL032626) predicted using
Genefinder~contains similarity to Pfam domain: PF00282
(Pyridoxal-dependent decarboxylase conserved domain),
Score=509.1, E-value=1.1e-149, N=1~cDNA EST EMBL:Z14925
comes from this gene~cDNA EST yk387h9.3 comes from this
gene> >gi|4406374|gb|AAD19958.1| (AF109378) glutamic
acid decarboxylase [Caenorhabditis elegans]
Length = 508
Score = 69.5 bits (167), Expect = 6e-11
Identities = 75/296 (25%), Positives = 123/296 (41%), Gaps = 46/296 (15%)
Query: 84 EKGYGHIVSGGTEANILAVRAFRN----------ISDAERPELILPKSAHFSFIKAGEML 133
EK G GG AN+ A+ A R+ + D + +H+S A +L
Sbjct: 157 EKADGIFAPGGAIANLYAMNAARHQLWPRSKHLGMKDIPTLCCFTSEDSHYSIKSASAVL 216
Query: 134 GVKLVWA-ELKQDY-------AVDVKDVEAKISDNT-IGIVGIAGTTGLGVVDDIPALSD 184
G+ + + D A++ K +E K T AG+T G D + +++
Sbjct: 217 GIGADYCFNIPTDKNGKMIPEALEAKIIECKKEGLTPFFACCTAGSTVYGAFDPLERVAN 276
Query: 185 LAREYGIPLHVDAAFGGFVIPFAKSLGYDLPDFDFKLKGVE---SITIDPHKMGMAPIPA 241
+ + + HVDAA+GG ++ P+ +KL G+E S+T +PHK+ A +
Sbjct: 277 ICERHKLWFHVDAAWGGGMLLS--------PEHRYKLAGIERANSVTWNPHKLMGALLQC 328
Query: 242 GGIIFRRKKYLKAISVLA---------PYLAGGKVWQATITGTRPGASVLAVWALIKHLG 292
+FR+ L + ++ PY I R V +W + K G
Sbjct: 329 SACLFRQDGLLFQCNQMSADYLFQQDKPYDVSFDTGDKAIQCGRHN-DVFKLWLMWKSKG 387
Query: 293 FEGYREIVRKAMELSRWFAEEIKKLNNAWLVRE--PMLNI----VSFQTKNLRKVE 342
EGYR+ + K M+L+ +F IK+ L+ E LNI V + +NL E
Sbjct: 388 MEGYRQQINKLMDLANYFTRRIKETEGFELIIENPEFLNICFWYVPSKIRNLEPAE 443
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.141 0.415
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 146509345
Number of Sequences: 2977
Number of extensions: 6352530
Number of successful extensions: 17668
Number of sequences better than 1.0e-10: 73
Number of HSP's better than 0.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 55
Number of HSP's that attempted gapping in prelim test: 17436
Number of HSP's gapped (non-prelim): 75
length of query: 384
length of database: 189,106,746
effective HSP length: 54
effective length of query: 330
effective length of database: 156,788,448
effective search space: 51740187840
effective search space used: 51740187840
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 166 (69.1 bits)