BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1595 (sat) DE:sulfate adenylyltransferase (sat)
(379 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P56863|SAT_PYRAB SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 795 0.0
sp|Q9YCR4|SAT_AERPE SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 525 e-148
sp|O34764|SAT1_BACSU SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYL... 348 7e-95
dbj|BAB05206.1| (AP001512) sulfate adenylyltransferase [Bacillus... 345 6e-94
sp|O06736|SAT2_BACSU PROBABLE SULFATE ADENYLYLTRANSFERASE (SULFA... 333 3e-90
sp|P74241|SAT_SYNY3 SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 324 8e-88
dbj|BAB07105.1| (AP001518) sulfate adenylyltransferase [Bacillus... 323 3e-87
sp|P56864|SAT_DEIRA SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 314 1e-84
gb|AAK43020.1| Sulfate adenylyltransferase (sat) [Sulfolobus sol... 301 1e-80
pir||B69839 probable sulfate adenylyltransferase (EC 2.7.7.4) yi... 299 4e-80
sp|O76156|SAT_ENTHI SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 268 1e-70
sp|O67174|SATC_AQUAE PROBABLE BIFUNCTIONAL SAT/APS KINASE [INCLU... 231 1e-59
sp|Q12555|MET3_EMENI SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYL... 216 3e-55
sp|O28606|SAT_ARCFU SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 216 3e-55
gi|11499257 sulfate adenylyltransferase (sat) [Archaeoglobus ful... 216 3e-55
pir||A53651 sulfate adenylyltransferase (EC 2.7.7.4) [validated]... 210 2e-53
sp|P08536|MET3_YEAST SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYL... 207 1e-52
gi|6322469 ATP sulfurylase; Met3p [Saccharomyces cerevisiae] >gi... 207 1e-52
sp|Q12650|MET3_PENCH SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYL... 206 3e-52
sp|P78937|MET3_SCHPO SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYL... 205 8e-52
sp|O66036|SAT_CHRVI SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 204 1e-51
sp|P56862|MET3_ASPTE SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYL... 201 9e-51
sp|Q9Y872|MET3_CANAL SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYL... 195 6e-49
pir||T08094 probable sulfate adenylyltransferase (EC 2.7.7.4) AT... 149 6e-35
dbj|BAB00629.1| (AB036852) ATP sulfurylase/APS kinase [Ciona int... 127 2e-28
sp|Q54506|SAT_RIFPS SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLA... 127 3e-28
pir||T24918 3'-phosphoadenosine-5'-phosphosulfate synthetase - C... 126 5e-28
gb|AAF19185.1|AF198964_1 (AF198964) ATP sulfurylase [Arabidopsis... 124 1e-27
emb|CAA11417.1| (AJ223499) ATP sulfurylase [Brassica juncea] 124 2e-27
gb|AAA21570.1| (U05218) ATP sulfurylase [Arabidopsis thaliana] 123 2e-27
pir||S44267 sulfate adenylyltransferase (EC 2.7.7.4) met3-2 - po... 123 3e-27
gb|AAD26634.1| (AF110407) ATP sulfurylase precursor [Arabidopsis... 122 5e-27
pir||S68201 sulfate adenylyltransferase (EC 2.7.7.4) precursor (... 122 7e-27
gb|AAB09473.1| (U59738) ATP sulfurylase [Arabidopsis thaliana] 122 7e-27
pir||E71409 sulfate adenylyltransferase (EC 2.7.7.4) precursor (... 122 7e-27
gb|AAF20366.1|AF150754_1 (AF150754) 3'phosphoadenosine 5'-phosph... 122 7e-27
gb|AAD38423.1|AF074331_1 (AF074331) PAPS synthetase-2 [Homo sapi... 122 9e-27
pir||T08594 probable sulfate adenylyltransferase (EC 2.7.7.4) - ... 121 2e-26
gb|AAF49102.1| (AE003515) Paps gene product [Drosophila melanoga... 121 2e-26
emb|CAA73368.1| (Y12861) bifunctional ATP sulfurylase/APS kinase... 120 3e-26
gi|4758880 3-prime-phosphoadenosine 5-prime-phosphosulfate synth... 120 3e-26
gb|AAF12761.1|AF173365_1 (AF173365) ATP sulfurylase/APS kinase i... 120 3e-26
pir||S44079 sulfate adenylyltransferase (EC 2.7.7.4) met3-1 - po... 120 4e-26
pir||JC4383 3'-phosphoadenosine-5'-phosphosulfate synthetase - s... 120 4e-26
gb|AAC98687.1| (AF085144) ATP sulfurylase/APS kinase 2; PAPS syn... 120 4e-26
gb|AAF40307.2| (AF160509) 3'-phosphoadenosine 5'-phosphosulfate ... 120 4e-26
gi|11431665 3-prime-phosphoadenosine 5-prime-phosphosulfate synt... 120 4e-26
emb|CAA11416.1| (AJ223498) ATP sulfurylase [Brassica juncea] 118 8e-26
gb|AAF70194.1| (AF251798) adenosine 5'-phosphosulfate kinase/ATP... 118 1e-25
pir||T40044 probable sulfate adenylyltransferase - fission yeast... 116 4e-25
gb|AAF18998.1|AF212154_1 (AF212154) ATP-sulfurylase [Allium cepa] 116 4e-25
sp|O88428|PPS2_MOUSE BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHO... 116 5e-25
gi|6754984 3'-phosphoadenosine 5'-phosphosulfate synthase 2 [Mus... 115 1e-24
pir||S44943 sulfate adenylyltransferase (EC 2.7.7.4) met3-1 prec... 114 2e-24
pir||T14475 sulfate adenylyltransferase (EC 2.7.7.4) ASBo precur... 113 3e-24
gb|AAF13064.1|AF195511_1 (AF195511) ATP sulfurylase precursor [B... 113 3e-24
gi|6754982 3'-phosphoadenosine 5'-phosphosulfate synthase 1 [Mus... 111 1e-23
pir||JW0087 3'-phosphoadenosine-5'-phosphosulfate synthetase - h... 111 1e-23
sp|O43252|PPS1_HUMAN BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHO... 111 1e-23
emb|CAB89007.1| (AL353866) sulfate adenylyltransferase; methioni... 111 2e-23
gi|4885537 3-prime-phosphoadenosine 5-prime-phosphosulfate synth... 110 2e-23
pir||S68202 ATP sulfurylase (clone APS2) precursor - Arabidopsis... 109 5e-23
gb|AAF40236.1|AF105227_1 (AF105227) 3'-phosphoadenosine 5'-phosp... 109 5e-23
gb|AAD09325.1| (AF016496) ATP sulfurylase/APS kinase [Homo sapiens] 106 5e-22
sp|O54820|PPS1_CAVPO BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHO... 106 6e-22
pir||T01204 sulfate adenylyltransferase (EC 2.7.7.4) - maize >gi... 104 2e-21
dbj|BAA36274.1| (AB015204) plastidic ATP sulfurylase [Oryza sativa] 104 2e-21
gb|AAF12780.1| (AF173364) ATP sulfurylase/APS kinase isoform SK2... 76 6e-13
pir||G81256 probable sulfate adenylyltransferase (EC 2.7.7.4) Cj... 70 4e-11
>sp|P56863|SAT_PYRAB SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|7438199|pir||D75097 sulfate
adenylyltransferase (sat) PAB1595 - Pyrococcus abyssi
(strain Orsay) >gi|5458593|emb|CAB50081.1| (AJ248286)
sulfate adenylyltransferase (sat) [Pyrococcus abyssi]
Length = 379
Score = 795 bits (2032), Expect = 0.0
Identities = 379/379 (100%), Positives = 379/379 (100%)
Query: 1 MVSKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTR 60
MVSKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTR
Sbjct: 1 MVSKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTR 60
Query: 61 EDFESVLDHMRLSDDTPWTIPIVLDVEKPEFEEGDAILLYHKETPIARMHVEDIYTYEKE 120
EDFESVLDHMRLSDDTPWTIPIVLDVEKPEFEEGDAILLYHKETPIARMHVEDIYTYEKE
Sbjct: 61 EDFESVLDHMRLSDDTPWTIPIVLDVEKPEFEEGDAILLYHKETPIARMHVEDIYTYEKE 120
Query: 121 EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEKG 180
EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEKG
Sbjct: 121 EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEKG 180
Query: 181 WKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEH 240
WKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEH
Sbjct: 181 WKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEH 240
Query: 241 YYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDL 300
YYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDL
Sbjct: 241 YYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDL 300
Query: 301 FDEFPDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEY 360
FDEFPDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEY
Sbjct: 301 FDEFPDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEY 360
Query: 361 MMRPEVYEVIRSFDNPFVE 379
MMRPEVYEVIRSFDNPFVE
Sbjct: 361 MMRPEVYEVIRSFDNPFVE 379
>sp|Q9YCR4|SAT_AERPE SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|7438198|pir||A72591 probable
sulfate adenylyltransferase APE1197 - Aeropyrum pernix
(strain K1) >gi|5104869|dbj|BAA80183.1| (AP000061) 389aa
long hypothetical sulfate adenylyltransferase [Aeropyrum
pernix]
Length = 389
Score = 525 bits (1338), Expect = e-148
Identities = 241/383 (62%), Positives = 302/383 (77%), Gaps = 5/383 (1%)
Query: 1 MVSKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTR 60
+VS+PHGG+L+RR+ + R RE S+ E P++++ RAID E++A GV+SPL+GF+
Sbjct: 7 LVSRPHGGRLVRRVLSGRRREIFESQYREMPRLEVPLERAIDAEDLARGVFSPLEGFMVE 66
Query: 61 EDFESVLDHMRLSDDTPWTIPIVLDVEKP-----EFEEGDAILLYHKETPIARMHVEDIY 115
+D+ SVL MRLS+D PWTIPIVLD + GD I+L + PIA + +EDIY
Sbjct: 67 DDYLSVLSRMRLSNDLPWTIPIVLDANREWVLNEGVSAGDDIILTYHGLPIAVLTLEDIY 126
Query: 116 TYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVL 175
+++K A KVFKT+D NHPGV Y G L+GG +EL+ PNP +YTL P+ETRVL
Sbjct: 127 SWDKGLHAEKVFKTRDPNHPGVEATYKRGDILLGGRLELIQGPPNPLERYTLWPVETRVL 186
Query: 176 FKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYE 235
FKEKGW+TV AFQTRNVPHLGHEYVQKAALTFVDGL ++P+ G KKRGDY+DEVII+AYE
Sbjct: 187 FKEKGWRTVAAFQTRNVPHLGHEYVQKAALTFVDGLLVHPLAGWKKRGDYRDEVIIRAYE 246
Query: 236 VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPY 295
L HYYP+ V VL+ +R M YAGPREA+HHAI+RKNFGATHFIVGRDHAGVG+YYGPY
Sbjct: 247 ALITHYYPRGVVVLSVLRMNMNYAGPREAVHHAIVRKNFGATHFIVGRDHAGVGSYYGPY 306
Query: 296 EAWDLFDEFPDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGE 355
EAW++F EFPDLGITP+F+REA+YC++CGGMVNEK+CPH ++Y VRISGT+LR M+ RGE
Sbjct: 307 EAWEIFREFPDLGITPLFVREAYYCRRCGGMVNEKVCPHGDEYRVRISGTRLREMLGRGE 366
Query: 356 KPPEYMMRPEVYEVIRSFDNPFV 378
+PPEYMMRPEV + I S +PF+
Sbjct: 367 RPPEYMMRPEVADAIISHPDPFI 389
>sp|O34764|SAT1_BACSU SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|7438195|pir||B69877 probable
sulfate adenylyltransferase (EC 2.7.7.4) ylnB - Bacillus
subtilis >gi|2462958|emb|CAA04411.1| (AJ000974) putative
sulfate adenylyltransferase [Bacillus subtilis]
>gi|2633932|emb|CAB13433.1| (Z99112) similar to sulfate
adenylyltransferase [Bacillus subtilis]
Length = 382
Score = 348 bits (883), Expect = 7e-95
Identities = 172/371 (46%), Positives = 234/371 (62%), Gaps = 11/371 (2%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFE 64
PHGG L+ R+ I E +++D DLE I G YSP++GF +D+
Sbjct: 5 PHGGTLVNRVDESYDVSGIQKE------IELDLISFADLELIGIGAYSPIEGFFNEKDYV 58
Query: 65 SVLDHMRLSDDTPWTIPIVLDVEKPEFEE---GDAILLYHKETPIARMHVEDIYTYEKEE 121
SV+++MRLS W++PI L V+ + E G+ + L ++ + +ED+Y +K++
Sbjct: 59 SVVENMRLSSGVVWSLPITLPVDAQKAAELSLGETVKLTYEGETYGVIQIEDLYVPDKQK 118
Query: 122 FALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEKGW 181
A+ V+KT + HPGV K++S G VGG I L+ + F ++T P ETR F EKGW
Sbjct: 119 EAVNVYKTDEQEHPGVKKLFSRGNTYVGGPITLIKKASKQFPEFTFEPSETRRQFAEKGW 178
Query: 182 KTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEHY 241
+T+V FQTRN H HEY+QK AL VDGLF+NP++G K D +V +++Y+VL +HY
Sbjct: 179 ETIVGFQTRNPVHRAHEYIQKTALETVDGLFLNPLVGETKSDDIPADVRMESYQVLLDHY 238
Query: 242 YPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDLF 301
YPKD L MRYAGPREAI HA++RKN+G THFIVGRDHAGVG+YYG YEA +LF
Sbjct: 239 YPKDRVFLGVFLAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVGDYYGTYEAQELF 298
Query: 302 DEF--PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPE 359
D F +LGITP+ +F+CKKCG M K CPH ++HV +SGTK+R M+ G PP
Sbjct: 299 DTFKPEELGITPLKFEHSFFCKKCGNMGTAKTCPHGREHHVILSGTKVRGMLRDGVLPPA 358
Query: 360 YMMRPEVYEVI 370
R EV EV+
Sbjct: 359 EFSRKEVVEVL 369
>dbj|BAB05206.1| (AP001512) sulfate adenylyltransferase [Bacillus halodurans]
Length = 381
Score = 345 bits (875), Expect = 6e-94
Identities = 175/375 (46%), Positives = 232/375 (61%), Gaps = 12/375 (3%)
Query: 2 VSKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTRE 61
+S+PHGG L++R E + +++D DLE I G +SPL GFLT +
Sbjct: 3 LSQPHGGTLVQRFHPEANVESVEKS------IELDAFSLSDLELIGIGAFSPLTGFLTEK 56
Query: 62 DFESVLDHMRLSDDTPWTIPIVLDVEKPE---FEEGDAILLYHKETPIARMHVEDIYTYE 118
D+ SV++ MRL + T W+IPI L V + + GD + L H+ + +EDIY +
Sbjct: 57 DYRSVVESMRLENGTVWSIPIALPVTEEKAATLSVGDVVKLDHQGETYGLLTIEDIYQPD 116
Query: 119 KEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNP-FAKYTLRPIETRVLFK 177
KE A V++T D HPGV K++ +GG I L + F+ Y L P ETR +F
Sbjct: 117 KELEAENVYRTTDLAHPGVKKLFDRPNVYIGGPITLTKRVERTKFSSYYLDPKETRAVFA 176
Query: 178 EKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVL 237
EKGWK VV FQTRN H HEY+QK AL VDGLF+NP++G K D +V +++YEVL
Sbjct: 177 EKGWKRVVGFQTRNPVHRAHEYIQKTALEIVDGLFLNPLVGETKADDIPADVRMESYEVL 236
Query: 238 FEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEA 297
E YYP+D LA MRYAGPREA+ HA++RKN+G THFIVGRDHAGVG+YYG Y+A
Sbjct: 237 LEKYYPQDRVFLAVFPAAMRYAGPREAVFHALVRKNYGCTHFIVGRDHAGVGDYYGTYDA 296
Query: 298 WDLFDEF--PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGE 355
+F F +LGITP+F +FYCK CG M + K CPH +++HV +SGTK+R M+ G
Sbjct: 297 QLIFHHFTREELGITPLFFEHSFYCKACGNMASTKTCPHGKEHHVILSGTKVREMLANGV 356
Query: 356 KPPEYMMRPEVYEVI 370
PP RPEV V+
Sbjct: 357 TPPPEFSRPEVVNVL 371
>sp|O06736|SAT2_BACSU PROBABLE SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE
TRANSFERASE) (SAT) (ATP-SULFURYLASE)
Length = 389
Score = 333 bits (844), Expect = 3e-90
Identities = 166/373 (44%), Positives = 228/373 (60%), Gaps = 11/373 (2%)
Query: 3 SKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTRED 62
++PHGG LI R E + ++D DLE IA G YSPL GFL +D
Sbjct: 4 NEPHGGVLINRCDPACHFEGCACQ------AELDQLALSDLELIAIGGYSPLTGFLGEKD 57
Query: 63 FESVLDHMRLSDDTPWTIPIVLDVEKP---EFEEGDAILLYHKETPIARMHVEDIYTYEK 119
+ SV+ MRL++ PW++PI L V + + GD + L + V DIY +K
Sbjct: 58 YHSVVKEMRLANGLPWSLPITLPVGEKTARQLSAGDHVKLVKDGVTYGMITVTDIYQPDK 117
Query: 120 EEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEK 179
+ AL VFKT D HPGV K+ + Y +GG I + + F ++ P ETR F++
Sbjct: 118 TQEALSVFKTNDPAHPGVKKLLARPDYYIGGPITVSSLPDKSFEQFYATPAETRAAFQKL 177
Query: 180 GWKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFE 239
GWKT+V FQTRN H HEY+QK AL VDGL ++P++G K D ++ +++Y+ L
Sbjct: 178 GWKTIVGFQTRNPVHRAHEYIQKTALETVDGLLLHPLVGETKSDDIPSDIRMESYQALLN 237
Query: 240 HYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWD 299
HYYPKD +L+ MRYAGPREAI HA++RKN+G THFIVGRDHAGVG+YYG Y+A +
Sbjct: 238 HYYPKDRVMLSVFPAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVGSYYGTYDAQN 297
Query: 300 LFDEF--PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKP 357
+F F +LGI P+F +FYC+KCG M K CPH + H+ +SGTK+R ++ +G+KP
Sbjct: 298 IFQSFTEEELGIKPLFFEHSFYCRKCGNMGTSKTCPHSPRDHIHLSGTKVRELLRQGKKP 357
Query: 358 PEYMMRPEVYEVI 370
P+ RPEV V+
Sbjct: 358 PKEFSRPEVAAVL 370
>sp|P74241|SAT_SYNY3 SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|7428529|pir||S75876 sulfate
adenylyltransferase (EC 2.7.7.4) - Synechocystis sp.
(strain PCC 6803) >gi|1653421|dbj|BAA18335.1| (D90913)
sulfate adenylyltransferase [Synechocystis sp.]
Length = 390
Score = 324 bits (823), Expect = 8e-88
Identities = 168/376 (44%), Positives = 232/376 (61%), Gaps = 8/376 (2%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFE 64
PHGG+LI R+A R+ L+ + P+VQ+D DL IA G +SPLKGF+ ++D+E
Sbjct: 10 PHGGQLINRLAPEAERQEFLAIADKLPRVQLDERATSDLVMIAIGGFSPLKGFMEQDDYE 69
Query: 65 SVLDHMRLSDDTPWTIPIVLDVEKP---EFEEGDAILLYHKETP-IARMHVEDIYTYEKE 120
V++ M+LS+ PW++P+ L V + E G + L + I + + Y Y K
Sbjct: 70 LVVEEMKLSNGLPWSVPVTLSVTEEVAAPLEVGSWVRLDNSAGKFIGVLELTQKYHYNKA 129
Query: 121 EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNP-FAKYTLRPIETRVLFKEK 179
A V++T D HPGV +Y G + G I LL P+P F KY + P +R LF E+
Sbjct: 130 HEAKNVYRTDDQAHPGVKVIYDQGPVNLAGPIWLLEREPHPLFPKYQIDPAASRQLFAER 189
Query: 180 GWKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFE 239
GWKT+V FQTRN H HEY+QK AL VDGLF++P++G K D +V ++ YE++ +
Sbjct: 190 GWKTIVGFQTRNPIHRAHEYIQKCALEVVDGLFLHPLVGATKSDDIPADVRMRCYEIMVD 249
Query: 240 HYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWD 299
+Y+PK+ +L MRYAGPREAI HA++RKN+G THFIVGRDHAGVG+YYG Y+A +
Sbjct: 250 NYFPKERVILGINPSAMRYAGPREAIFHALIRKNYGCTHFIVGRDHAGVGDYYGTYDAQE 309
Query: 300 LFDEFPD--LGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKP 357
+FDEF LGI PM AFYCKK M K P + + +SGTK+R ++ G+ P
Sbjct: 310 IFDEFAPEALGIVPMKFEHAFYCKKTLQMATTKTSPSGPEDRIHLSGTKVRALLRDGQLP 369
Query: 358 PEYMMRPEV-YEVIRS 372
P RPEV E+IR+
Sbjct: 370 PPEFSRPEVAQELIRA 385
>dbj|BAB07105.1| (AP001518) sulfate adenylyltransferase [Bacillus halodurans]
Length = 379
Score = 323 bits (818), Expect = 3e-87
Identities = 161/376 (42%), Positives = 234/376 (61%), Gaps = 13/376 (3%)
Query: 2 VSKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTRE 61
+S PHGG LI R + +E +++D DLE IA+G YSPL GF+ ++
Sbjct: 3 LSIPHGGSLIDRTKTSLDVSTLSAE------IELDSIAFSDLELIANGAYSPLTGFMNKK 56
Query: 62 DFESVLDHMRLSDDTPWTIPIVLDVEKPEFEE---GDAILLYHKETPIARMHVEDIYTYE 118
D+ESV+ MRL+D T W++PI L + + E GD + L + +T +++++++ +
Sbjct: 57 DYESVVTTMRLADGTVWSLPITLPITEETAESLRIGDQVKLTYNQTVYGVLYLKELFVPD 116
Query: 119 KEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELP--NPFAKYTLRPIETRVLF 176
K A+ VF+T D +HPGV +++ G G I L+ + + + Y P ETR F
Sbjct: 117 KTLEAMHVFQTTDHDHPGVQRLFKRGDVYAAGPIHLIQQPARNDQWTHYYRSPKETRAEF 176
Query: 177 KEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEV 236
+ W +VV FQTRN H HEY+QK AL VDGL ++P++G K+ D + +++Y+V
Sbjct: 177 SRRQWNSVVGFQTRNPVHRAHEYIQKCALESVDGLLLHPLVGDTKKDDIPAAIRMESYDV 236
Query: 237 LFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYE 296
L +HYY KD L+ MRYAGPREA+ HAI+RKN+G THFIVGRDHAGVG+YYG Y+
Sbjct: 237 LLKHYYAKDRVYLSVFPAAMRYAGPREAVFHAIVRKNYGCTHFIVGRDHAGVGDYYGTYD 296
Query: 297 AWDLFDEFP--DLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRG 354
A +F +FP +LGI + +FYCKKCG M + K CPH +++H+ +SGTK+R M+ G
Sbjct: 297 AQQVFTQFPQAELGIHILPFEHSFYCKKCGSMASAKTCPHKKEHHLHLSGTKVREMLRSG 356
Query: 355 EKPPEYMMRPEVYEVI 370
KPP RPEV +V+
Sbjct: 357 VKPPPEFSRPEVIDVL 372
>sp|P56864|SAT_DEIRA SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|7473946|pir||D75594 sulfate
adenylyltransferase - Deinococcus radiodurans (strain
R1) >gi|6460578|gb|AAF12284.1|AE001862_110 (AE001862)
sulfate adenylyltransferase [Deinococcus radiodurans]
Length = 387
Score = 314 bits (796), Expect = 1e-84
Identities = 156/370 (42%), Positives = 229/370 (61%), Gaps = 4/370 (1%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFE 64
P GG L+RRI P ++ +E P++++ DLE IA G YSPL GF+ D+
Sbjct: 14 PLGGSLVRRIWRPG-QDFDPAELAGRPRLELSSRSLADLEMIATGAYSPLTGFVGEADYL 72
Query: 65 SVLDHMRLSDDTPWTIPIVLDVEKPEFEE-GDAILLYHKETPIARMHVEDIYTYEKEEFA 123
S+++H+RL+D TPW++PI L V + ++L H P+ + +E+ Y +K A
Sbjct: 73 SIIEHLRLADGTPWSLPITLPVTAEQAAGLSGRVVLTHGGEPVGTLDIEEKYAAQKSLEA 132
Query: 124 LKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEKGWKT 183
+V++T++ HPGVA +Y+ G + G + L F + P E R + + +GW++
Sbjct: 133 REVYRTEEEAHPGVAALYAQGDVYLAGPVTLFEVPRGEFPRAHRTPAEVREVIEARGWRS 192
Query: 184 VVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEHYYP 243
VAFQTRN H HEY+QK AL VDGL ++P++G+ K D E ++AYEVL YYP
Sbjct: 193 TVAFQTRNPIHRAHEYLQKVALELVDGLLLHPLVGQTKGDDVPAETRMEAYEVLLRGYYP 252
Query: 244 KDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDLFDE 303
++ +L+ MRYAGPREAI HA+ R+N+GATHFIVGRDHAGVG+YYG Y+A ++F+
Sbjct: 253 QERTLLSVYPAAMRYAGPREAIVHALSRRNYGATHFIVGRDHAGVGSYYGTYDAQEIFNT 312
Query: 304 F--PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYM 361
+ +LGI + FYC+ CG +V+ + CPHD +H+ +SGTK+R + GE P
Sbjct: 313 YTAEELGIRILKFEHTFYCQSCGQLVSPRTCPHDSSHHLVLSGTKVREKLRAGENLPPEF 372
Query: 362 MRPEVYEVIR 371
RPEV EV+R
Sbjct: 373 TRPEVAEVLR 382
>gb|AAK43020.1| Sulfate adenylyltransferase (sat) [Sulfolobus solfataricus]
Length = 406
Score = 301 bits (762), Expect = 1e-80
Identities = 162/383 (42%), Positives = 240/383 (62%), Gaps = 27/383 (7%)
Query: 20 RERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDDTPWT 79
R + +S+ E ++++ A ++ +IA+G SPLKGF+ E+ + V+++MRL + W
Sbjct: 14 RIKTISDFKELHRIEVKRQLAHEIVSIAYGFLSPLKGFMNYEEVDGVVENMRLPNGVLWP 73
Query: 80 IPIVLDVEKPE-FEEGDAILLYHKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVA 138
IP+V D + E +EGD I + + P+A M V++I+ Y+K + A KV+KTKD HPGV
Sbjct: 74 IPLVFDYSQNEKVKEGDTIGITYLGKPLAIMKVKEIFKYDKLKIAEKVYKTKDIKHPGVK 133
Query: 139 KVYSMGKYLVGGEIELLNE--LPNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLG 196
+ S + G++ L+ E P++++ L P R +F++KGWK VVAFQTRNVPH G
Sbjct: 134 RTLSYADAFLAGDVWLVREPQFNKPYSEFWLTPRMHRTVFEKKGWKRVVAFQTRNVPHTG 193
Query: 197 HEYVQKAALTFVD----------GLFINPVLGRKKRGDYKDEVIIKAYEVLFEHYY--PK 244
HEY+ K A + G+ +N V+G K+ GDY DE I+ ++ L ++ Y PK
Sbjct: 194 HEYLMKFAWFAANENQKVDEPRTGILVNVVIGEKRVGDYIDEAILLTHDALSKYGYISPK 253
Query: 245 DVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDLFDEF 304
V +L+ ++MRYAGPREA+ HAI+R N G TH + GRDHAGVGNYY PYEA ++FD
Sbjct: 254 -VHLLSFTLWDMRYAGPREALLHAIIRSNLGCTHHVFGRDHAGVGNYYSPYEAHEIFDSI 312
Query: 305 --PDLGITPMFIREAFYCKKCGGMVNEKICPH-DEKYHVRISGTKLRNMIMRGEKPPEYM 361
DL I P+F+RE +YC +CG + NE +C H DEK SG+ +R++I+ KP + +
Sbjct: 313 NEEDLLIKPIFLRENYYCPRCGSIENEILCDHKDEKQ--EFSGSLIRSIILDEVKPTKMV 370
Query: 362 MRPEVYEVIR------SFDNPFV 378
MRPEVY+V+ F +PFV
Sbjct: 371 MRPEVYDVLMKAAEQYGFGSPFV 393
>pir||B69839 probable sulfate adenylyltransferase (EC 2.7.7.4) yitA - Bacillus
subtilis >gi|2145393|emb|CAA70656.1| (Y09476) YitA
[Bacillus subtilis] >gi|2633428|emb|CAB12932.1| (Z99109)
similar to sulfate adenylyltransferase [Bacillus
subtilis]
Length = 325
Score = 299 bits (757), Expect = 4e-80
Identities = 141/306 (46%), Positives = 195/306 (63%), Gaps = 5/306 (1%)
Query: 70 MRLSDDTPWTIPIVLDVEKP---EFEEGDAILLYHKETPIARMHVEDIYTYEKEEFALKV 126
MRL++ PW++PI L V + + GD + L + V DIY +K + AL V
Sbjct: 1 MRLANGLPWSLPITLPVGEKTARQLSAGDHVKLVKDGVTYGMITVTDIYQPDKTQEALSV 60
Query: 127 FKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEKGWKTVVA 186
FKT D HPGV K+ + Y +GG I + + F ++ P ETR F++ GWKT+V
Sbjct: 61 FKTNDPAHPGVKKLLARPDYYIGGPITVSSLPDKSFEQFYATPAETRAAFQKLGWKTIVG 120
Query: 187 FQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDV 246
FQTRN H HEY+QK AL VDGL ++P++G K D ++ +++Y+ L HYYPKD
Sbjct: 121 FQTRNPVHRAHEYIQKTALETVDGLLLHPLVGETKSDDIPSDIRMESYQALLNHYYPKDR 180
Query: 247 AVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDLFDEF-- 304
+L+ MRYAGPREAI HA++RKN+G THFIVGRDHAGVG+YYG Y+A ++F F
Sbjct: 181 VMLSVFPAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVGSYYGTYDAQNIFQSFTE 240
Query: 305 PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRP 364
+LGI P+F +FYC+KCG M K CPH + H+ +SGTK+R ++ +G+KPP+ RP
Sbjct: 241 EELGIKPLFFEHSFYCRKCGNMGTSKTCPHSPRDHIHLSGTKVRELLRQGKKPPKEFSRP 300
Query: 365 EVYEVI 370
EV V+
Sbjct: 301 EVAAVL 306
>sp|O76156|SAT_ENTHI SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|3551262|dbj|BAA32829.1| (AB013399)
ATP sulfurylase [Entamoeba histolytica]
Length = 423
Score = 268 bits (677), Expect = 1e-70
Identities = 143/388 (36%), Positives = 217/388 (55%), Gaps = 18/388 (4%)
Query: 4 KPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDF 63
KPHGGKLI+ + R+ + E PK+ I DL + G +SPL GF+ +ED+
Sbjct: 12 KPHGGKLIKTLKYGAERDECIKEAKSLPKIDISSREFGDLVMMGIGGFSPLNGFMKKEDW 71
Query: 64 ESVLDHMRLSDDTPWTIPIVLDVEKPE---FEEGDAILL-YHKETP--IARMHVEDIYTY 117
SV + L+D T W IPI + V + E + G + L Y+K+ + ++ +Y
Sbjct: 72 FSVCKNFTLADGTFWPIPITMSVSEEEAKKLKRGQKVALKYNKDINDISGTIEIDQVYEM 131
Query: 118 EKEEFALK---VFKTKDANHPGVAKVYSMGKYLVGGEIELLNE--LPNPFAKYTLRPIET 172
K++ ++ +F T D++HPGV KV + V G++ L+E P + + P E+
Sbjct: 132 TKKDKEMECKDIFTTTDSDHPGVKKVMEQKPFNVAGKVTTLSEGEFPIKYKGIYMTPEES 191
Query: 173 RVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIK 232
R+ F +KGWKT+ A Q RN H HE++ K A+ DG+FI+ ++G K GD EV +K
Sbjct: 192 RLNFAKKGWKTIAALQLRNPMHRSHEFLAKIAVEVCDGVFIHSLVGNLKPGDIPAEVRVK 251
Query: 233 AYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYY 292
+ L + Y+ K + +MRY GPREA+ HA R+N+G TH I+GRDHAGVG+YY
Sbjct: 252 CIDALVDKYFVKKNVLQGGYPLDMRYGGPREALLHATFRQNYGCTHMIIGRDHAGVGDYY 311
Query: 293 GPYEAWDLFDEFP-------DLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGT 345
GP++A +FD+ P L PM I FYC KC GM + + CPH +K V +SGT
Sbjct: 312 GPFDAQKIFDKIPYNADPKKRLLTQPMKIDWTFYCHKCDGMASLRTCPHTKKDRVIVSGT 371
Query: 346 KLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
+R M+ G+ P++ R E +++ +
Sbjct: 372 MVRKMLSEGKTLPDHFGRAESLKILADY 399
>sp|O67174|SATC_AQUAE PROBABLE BIFUNCTIONAL SAT/APS KINASE [INCLUDES: SULFATE
ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE)
(SAT) (ATP-SULFURYLASE); ADENYLYLSULFATE KINASE (APS
KINASE) (ADENOSINE-5'PHOSPHOSULFATE KINASE) (ATP
ADENOSINE-5'-PHOSPHOSULFATE 3'-PHOSPHOTRAN>
>gi|7428530|pir||C70393 probable
3'-phosphoadenosine-5'-phosphosulfate synthetase
[similarity] - Aquifex aeolicus
>gi|2983561|gb|AAC07134.1| (AE000722) sulfate
adenylyltransferase [Aquifex aeolicus]
Length = 546
Score = 231 bits (582), Expect = 1e-59
Identities = 140/361 (38%), Positives = 208/361 (56%), Gaps = 17/361 (4%)
Query: 26 EQHEYPK-VQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDDTPWTIPIVL 84
E+ +Y K +QI +DL+ +A G ++PL F+ ED+ +V++ MRL T + IPI L
Sbjct: 2 EKIKYLKSIQISQRSVLDLKLLAVGAFTPLDRFMGEEDYRNVVESMRLKSGTLFPIPITL 61
Query: 85 DVEKP---EFEEGDAILLYH-KETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKV 140
+EK + +EG+ I+L K P+A M VE++Y + E A V T D HP VA++
Sbjct: 62 PMEKEIAKDLKEGEWIVLRDPKNVPLAIMRVEEVYKWNLEYEAKNVLGTTDPRHPLVAEM 121
Query: 141 YSMGKYLVGGEIELLNELPN--PFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHE 198
++ G+Y + GE++++ +LP F +Y P + R K G +VAFQTRN H HE
Sbjct: 122 HTWGEYYISGELKVI-QLPKYYDFPEYRKTPKQVREEIKSLGLDKIVAFQTRNPMHRVHE 180
Query: 199 YVQKAALTFVDG-LFINPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDVAVLATVRYEMR 257
+ K A+ V G L ++PV+G K GD ++ Y+VL+E YY K +LA + MR
Sbjct: 181 ELTKRAMEKVGGGLLLHPVVGLTKPGDVDVYTRMRIYKVLYEKYYDKKKTILAFLPLAMR 240
Query: 258 YAGPREAIHHAIMRKNFGATHFIVGRDHAGVGN------YYGPYEAWDLFDEFPD-LGIT 310
AGPREA+ H I+R+N+GATHFIVGRDHA G +Y PYEA +LF ++ D +GI
Sbjct: 241 MAGPREALWHGIIRRNYGATHFIVGRDHASPGKDSKGKPFYDPYEAQELFKKYEDEIGIK 300
Query: 311 PMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLR-NMIMRGEKPPEYMMRPEVYEV 369
+ E Y + V ++ ISGT++R N + +G K PE+ RPEV E+
Sbjct: 301 MVPFEELVYVPELDQYVEINEAKKRNLKYINISGTEIRENFLKQGRKLPEWFTRPEVAEI 360
Query: 370 I 370
+
Sbjct: 361 L 361
>sp|Q12555|MET3_EMENI SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|1084969|pir||S55034 sulfate
adenylyltransferase (EC 2.7.7.4) - Emericella nidulans
>gi|572513|emb|CAA57891.1| (X82541) sulfate
adenylyltransferase [Aspergillus nidulellus]
Length = 574
Score = 216 bits (545), Expect = 3e-55
Identities = 144/396 (36%), Positives = 217/396 (54%), Gaps = 25/396 (6%)
Query: 1 MVSKPHGGKLIRRIA--APRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFL 58
M + PHGG L IA APR +++ +E P + + + DLE I +G +SPL+GF+
Sbjct: 1 MANTPHGGVLKDLIARDAPR-HDQLEAEAATLPSIVLTERQLCDLELIMNGGFSPLEGFM 59
Query: 59 TREDFESVLDHMRLSDDTPWTIPIVLDVEKPEFEE-----GDAILL--YHKETPIARMHV 111
++D++ V+ RL+D +++PI LD K E+ G + L + + +A + +
Sbjct: 60 NQKDYDGVVAESRLADGNLFSMPITLDASKAVIEQAGLKPGSRVTLRDFRDDRNLAILTI 119
Query: 112 EDIYTYEKEEFALKVFKTKDANHPGVAKVYS-MGKYLVGGEIELLNELPNPFAKYTLR-- 168
+DIY +KE+ A VF D HP + + + + +Y +GG++E +N+L N + LR
Sbjct: 120 DDIYRPDKEKEAKLVFGG-DPEHPAIKYLNTKVEEYYIGGKLEAVNKL-NHYDYVGLRYT 177
Query: 169 PIETRVLFKEKGWKTVVAFQTRNVPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGDYKD 227
P E R+ F + GW VVAFQTRN H H E +AA + I+PV+G K GD
Sbjct: 178 PAELRIHFDKLGWTRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDH 237
Query: 228 EVIIKAYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAG 287
++AY+ L Y P +A LA + MR GPREA+ HAI+RKN GATHFIVGRDHAG
Sbjct: 238 FTRVRAYQALLPRY-PNGMAALALLPLAMRMGGPREAVWHAIIRKNHGATHFIVGRDHAG 296
Query: 288 VG------NYYGPYEAWDLFDEF-PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHV 340
G +YGPY+A +++ +LGI + ++ Y + + P K +
Sbjct: 297 PGKNSKGQEFYGPYDAQHAVEKYRAELGIEVVEFQQVTYLPDTDEYMPKDEVPAGTK-TL 355
Query: 341 RISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNP 376
ISGT+LRN + G PE+ PEV +++R P
Sbjct: 356 DISGTELRNRLRTGAHIPEWFSYPEVVKILRESSPP 391
>sp|O28606|SAT_ARCFU SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|2586075|gb|AAC46388.1| (U66886)
dissimilatory sulfate adenylyltransferase [Archaeoglobus
fulgidus]
Length = 456
Score = 216 bits (545), Expect = 3e-55
Identities = 132/425 (31%), Positives = 218/425 (51%), Gaps = 52/425 (12%)
Query: 5 PHGGKLIRRIAAPRT-RERILS--EQHEYPKVQIDHGRAI-----DLENIAHGVYSPLKG 56
PHGGKL+ R+ R E++++ +E + G I ++ ++ +G +SP++G
Sbjct: 9 PHGGKLVERVVKKRDIAEKMIAGCPTYELKPTTLPDGTPIRHVYREIMSVCYGFFSPVEG 68
Query: 57 FLTREDFESVLDHMRLSDDTPWTIPIVLDVEKPEFE-----EGDAILLYHKETPIARMHV 111
+ + + E VL+ RL + + PI+ D+ + +++ EGD +LL K P A + +
Sbjct: 69 SMVQNELERVLNERRLLSEWIFPYPILFDISEEDYKALDVKEGDRLLLMLKGQPFATLDI 128
Query: 112 EDIYTYEKEEFALKVFKTKDAN-----------HPGVAKVYSMGKYLVGGEIELLNE--L 158
E++Y + + A + F T + N HPG ++ G+ ++NE
Sbjct: 129 EEVYKIDPVDVATRTFGTPEKNPEVVREPFDDKHPGYVIYKMHNPIILAGKYTIVNEPKF 188
Query: 159 PNPFAKYTLRPIETRVLFK-EKGWKTVVAFQTRNVPHLGHEYVQKAALTFVD-----GLF 212
P+ ++ P + R + K EK W+TV+A QTRNVPH+GHE + K A D G+
Sbjct: 189 KEPYDRFWFPPSKCREVIKNEKKWRTVIAHQTRNVPHVGHEMLMKCAAYTGDIEPCHGIL 248
Query: 213 INPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDVAVLATVR-YEMRYAGPREAIHHAIMR 271
+N ++G K+RGDY DE I++ +E + ++ Y K + T ++MRY P E++ H ++R
Sbjct: 249 VNAIIGAKRRGDYPDEAILEGHEAVNKYGYIKPERHMVTFTLWDMRYGNPIESLLHGVIR 308
Query: 272 KNFGATHFIVGRDHAGVGNYYGPYEAWDLFDE-FPDLG-------------ITPMFIREA 317
+N G TH + GRDHA VG YY Y L+ + P G I P + E
Sbjct: 309 QNMGCTHHMFGRDHAAVGEYYDMYATQILWSQGIPSFGFEAPPNEVDYGLKIIPQNMAEF 368
Query: 318 FYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI----RSF 373
+YC C + + C H + + SG+ LR M+ G PP +MRPEVY+ I + +
Sbjct: 369 WYCPICQEIAYSENCGHTDAKQ-KFSGSFLRGMVAEGVFPPRVVMRPEVYKQIVKWWKVY 427
Query: 374 DNPFV 378
+ PFV
Sbjct: 428 NYPFV 432
>gi|11499257 sulfate adenylyltransferase (sat) [Archaeoglobus fulgidus]
>gi|7483969|pir||B69458 sulfate adenylyltransferase
(sat) homolog - Archaeoglobus fulgidus
>gi|2648888|gb|AAB89581.1| (AE000988) sulfate
adenylyltransferase (sat) [Archaeoglobus fulgidus]
Length = 459
Score = 216 bits (545), Expect = 3e-55
Identities = 132/425 (31%), Positives = 218/425 (51%), Gaps = 52/425 (12%)
Query: 5 PHGGKLIRRIAAPRT-RERILS--EQHEYPKVQIDHGRAI-----DLENIAHGVYSPLKG 56
PHGGKL+ R+ R E++++ +E + G I ++ ++ +G +SP++G
Sbjct: 12 PHGGKLVERVVKKRDIAEKMIAGCPTYELKPTTLPDGTPIRHVYREIMSVCYGFFSPVEG 71
Query: 57 FLTREDFESVLDHMRLSDDTPWTIPIVLDVEKPEFE-----EGDAILLYHKETPIARMHV 111
+ + + E VL+ RL + + PI+ D+ + +++ EGD +LL K P A + +
Sbjct: 72 SMVQNELERVLNERRLLSEWIFPYPILFDISEEDYKALDVKEGDRLLLMLKGQPFATLDI 131
Query: 112 EDIYTYEKEEFALKVFKTKDAN-----------HPGVAKVYSMGKYLVGGEIELLNE--L 158
E++Y + + A + F T + N HPG ++ G+ ++NE
Sbjct: 132 EEVYKIDPVDVATRTFGTPEKNPEVVREPFDDKHPGYVIYKMHNPIILAGKYTIVNEPKF 191
Query: 159 PNPFAKYTLRPIETRVLFK-EKGWKTVVAFQTRNVPHLGHEYVQKAALTFVD-----GLF 212
P+ ++ P + R + K EK W+TV+A QTRNVPH+GHE + K A D G+
Sbjct: 192 KEPYDRFWFPPSKCREVIKNEKKWRTVIAHQTRNVPHVGHEMLMKCAAYTGDIEPCHGIL 251
Query: 213 INPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDVAVLATVR-YEMRYAGPREAIHHAIMR 271
+N ++G K+RGDY DE I++ +E + ++ Y K + T ++MRY P E++ H ++R
Sbjct: 252 VNAIIGAKRRGDYPDEAILEGHEAVNKYGYIKPERHMVTFTLWDMRYGNPIESLLHGVIR 311
Query: 272 KNFGATHFIVGRDHAGVGNYYGPYEAWDLFDE-FPDLG-------------ITPMFIREA 317
+N G TH + GRDHA VG YY Y L+ + P G I P + E
Sbjct: 312 QNMGCTHHMFGRDHAAVGEYYDMYATQILWSQGIPSFGFEAPPNEVDYGLKIIPQNMAEF 371
Query: 318 FYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI----RSF 373
+YC C + + C H + + SG+ LR M+ G PP +MRPEVY+ I + +
Sbjct: 372 WYCPICQEIAYSENCGHTDAKQ-KFSGSFLRGMVAEGVFPPRVVMRPEVYKQIVKWWKVY 430
Query: 374 DNPFV 378
+ PFV
Sbjct: 431 NYPFV 435
>pir||A53651 sulfate adenylyltransferase (EC 2.7.7.4) [validated] - Penicillium
chrysogenum
Length = 573
Score = 210 bits (530), Expect = 2e-53
Identities = 145/396 (36%), Positives = 213/396 (53%), Gaps = 25/396 (6%)
Query: 1 MVSKPHGGKLIRRIA--APRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFL 58
M + PHGG L +A APR E + +E P V + + DLE I +G +SPL+GF+
Sbjct: 1 MANAPHGGVLKDLLARDAPRQAE-LAAEAESLPAVTLTERQLCDLELIMNGGFSPLEGFM 59
Query: 59 TREDFESVLDHMRLSDDTPWTIPIVLD-----VEKPEFEEGDAILL--YHKETPIARMHV 111
+ D++ V + RL+D +++PI LD +++ + + I L + + +A + +
Sbjct: 60 NQADYDRVCEDNRLADGNVFSMPITLDASQEVIDEKKLQAASRITLRDFRDDRNLAILTI 119
Query: 112 EDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGK-YLVGGEIELLNELPNPFAKYTLR-- 168
+DIY +K + A VF D HP + + + K + +GG+IE +N+L N + LR
Sbjct: 120 DDIYRPDKTKEAKLVFGG-DPEHPAIVYLNNTVKEFYIGGKIEAVNKL-NHYDYVALRYT 177
Query: 169 PIETRVLFKEKGWKTVVAFQTRNVPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGDYKD 227
P E RV F + GW VVAFQTRN H H E +AA + + I+PV+G K GD
Sbjct: 178 PAELRVHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARSRQANVLIHPVVGLTKPGDIDH 237
Query: 228 EVIIKAYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAG 287
++AY+ L Y P +AVL + MR GPREAI HAI+RKN GATHFIVGRDHAG
Sbjct: 238 FTRVRAYQALLPRY-PNGMAVLGLLGLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAG 296
Query: 288 VG------NYYGPYEAWDLFDEFPD-LGITPMFIREAFYCKKCGGMVNEKICPHDEKYHV 340
G ++YGPY+A +++ D LGI + + Y P K +
Sbjct: 297 PGSNSKGEDFYGPYDAQHAVEKYKDELGIEVVEFQMVTYLPDTDEYRPVDQVPAGVK-TL 355
Query: 341 RISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNP 376
ISGT+LR + G PE+ PEV +++R + P
Sbjct: 356 NISGTELRRRLRSGAHIPEWFSYPEVVKILRESNPP 391
>sp|P08536|MET3_YEAST SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|3928|emb|CAA29702.1| (X06413) ATP
sulphurylase (AA 1 - 521) [Saccharomyces cerevisiae]
>gi|3937|emb|CAA42726.1| (X60157) ATP sulfurlase
(ATP:sulfate adenylyltransferase) [Saccharomyces
cerevisiae]
Length = 521
Score = 207 bits (522), Expect = 1e-52
Identities = 140/395 (35%), Positives = 213/395 (53%), Gaps = 31/395 (7%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKV---QIDHGRAIDLENIAHGVYSPLKGFLTRE 61
PHGG L IA ++ L + + + + + D+E I +G +SPL GFL
Sbjct: 4 PHGGILQDLIARDALKKNELLSEAQSSDILVWNLTPRQLCDIELILNGGFSPLTGFLNEN 63
Query: 62 DFESVLDHMRLSDDTPWTIPIVLDVEKP---EFEEGDAILLYHK-ETPIARMHVEDIYTY 117
D+ SV+ RL+D T WTIPI LDV++ + + I L+ E PIA + V+D+Y
Sbjct: 64 DYSSVVTDSRLADGTLWTIPITLDVDEAFANQIKPDTRIALFQDDEIPIAILTVQDVYKP 123
Query: 118 EKEEFALKVFKTKDANHPGVAKVYSM-GKYLVGGEIELLNELPNPFAKYTLR--PIETRV 174
K A KVF+ D HP ++ ++++ G Y VGG +E + +LP + LR P + R+
Sbjct: 124 NKTIEAEKVFRG-DPEHPAISYLFNVAGDYYVGGSLEAI-QLPQHYDYPGLRKTPAQLRL 181
Query: 175 LFKEKGWKTVVAFQTRNVPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKA 233
F+ + W VVAFQTRN H H E +AA + I+PV+G K GD ++
Sbjct: 182 EFQSRQWDRVVAFQTRNPMHRAHRELTVRAAREANAKVLIHPVVGLTKPGDIDHHTRVRV 241
Query: 234 YEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVG---- 289
Y+ + + Y P +A L+ + MR +G REA+ HAI+RKN+GA+HFIVGRDHAG G
Sbjct: 242 YQEIIKRY-PNGIAFLSLLPLAMRMSGDREAVWHAIIRKNYGASHFIVGRDHAGPGKNSK 300
Query: 290 --NYYGPYEAWDLFDEFP-DLGITPMFIREAFYCKKCGGMVNEKICPHDE-----KYHVR 341
++YGPY+A +L + + +L I + R Y ++ P D+ +
Sbjct: 301 GVDFYGPYDAQELVESYKHELDIEVVPFRMVTYLPD-----EDRYAPIDQIDTTKTRTLN 355
Query: 342 ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNP 376
ISGT+LR + G + PE+ PEV +++R + P
Sbjct: 356 ISGTELRRRLRVGGEIPEWFSYPEVVKILRESNPP 390
>gi|6322469 ATP sulfurylase; Met3p [Saccharomyces cerevisiae]
>gi|1078402|pir||S55198 sulfate adenylyltransferase (EC
2.7.7.4) YJR010w - yeast (Saccharomyces cerevisiae)
>gi|854583|emb|CAA60932.1| (X87611) ATP sulphurylase
[Saccharomyces cerevisiae] >gi|1015638|emb|CAA89532.1|
(Z49510) ORF YJR010w [Saccharomyces cerevisiae]
Length = 511
Score = 207 bits (522), Expect = 1e-52
Identities = 140/395 (35%), Positives = 213/395 (53%), Gaps = 31/395 (7%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKV---QIDHGRAIDLENIAHGVYSPLKGFLTRE 61
PHGG L IA ++ L + + + + + D+E I +G +SPL GFL
Sbjct: 4 PHGGILQDLIARDALKKNELLSEAQSSDILVWNLTPRQLCDIELILNGGFSPLTGFLNEN 63
Query: 62 DFESVLDHMRLSDDTPWTIPIVLDVEKP---EFEEGDAILLYHK-ETPIARMHVEDIYTY 117
D+ SV+ RL+D T WTIPI LDV++ + + I L+ E PIA + V+D+Y
Sbjct: 64 DYSSVVTDSRLADGTLWTIPITLDVDEAFANQIKPDTRIALFQDDEIPIAILTVQDVYKP 123
Query: 118 EKEEFALKVFKTKDANHPGVAKVYSM-GKYLVGGEIELLNELPNPFAKYTLR--PIETRV 174
K A KVF+ D HP ++ ++++ G Y VGG +E + +LP + LR P + R+
Sbjct: 124 NKTIEAEKVFRG-DPEHPAISYLFNVAGDYYVGGSLEAI-QLPQHYDYPGLRKTPAQLRL 181
Query: 175 LFKEKGWKTVVAFQTRNVPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKA 233
F+ + W VVAFQTRN H H E +AA + I+PV+G K GD ++
Sbjct: 182 EFQSRQWDRVVAFQTRNPMHRAHRELTVRAAREANAKVLIHPVVGLTKPGDIDHHTRVRV 241
Query: 234 YEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVG---- 289
Y+ + + Y P +A L+ + MR +G REA+ HAI+RKN+GA+HFIVGRDHAG G
Sbjct: 242 YQEIIKRY-PNGIAFLSLLPLAMRMSGDREAVWHAIIRKNYGASHFIVGRDHAGPGKNSK 300
Query: 290 --NYYGPYEAWDLFDEFP-DLGITPMFIREAFYCKKCGGMVNEKICPHDE-----KYHVR 341
++YGPY+A +L + + +L I + R Y ++ P D+ +
Sbjct: 301 GVDFYGPYDAQELVESYKHELDIEVVPFRMVTYLPD-----EDRYAPIDQIDTTKTRTLN 355
Query: 342 ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNP 376
ISGT+LR + G + PE+ PEV +++R + P
Sbjct: 356 ISGTELRRRLRVGGEIPEWFSYPEVVKILRESNPP 390
>sp|Q12650|MET3_PENCH SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|460637|gb|AAA20839.1| (U07353) ATP
sulfurylase [Penicillium chrysogenum]
Length = 572
Score = 206 bits (519), Expect = 3e-52
Identities = 145/396 (36%), Positives = 213/396 (53%), Gaps = 26/396 (6%)
Query: 1 MVSKPHGGKLIRRIA--APRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFL 58
M + PHGG L +A APR E + +E P V + + DLE I +G +SPL+GF+
Sbjct: 1 MANAPHGGVLKDLLARDAPRQAE-LAAEAESLPAVTLTERQLCDLELIMNGGFSPLEGFM 59
Query: 59 TREDFESVLDHMRLSDDTPWTIPIVLD-----VEKPEFEEGDAILL--YHKETPIARMHV 111
+ D++ V + RL+D +++PI LD +++ + + I L + + +A + +
Sbjct: 60 NQADYDRVCEDNRLADGNVFSMPITLDASQEVIDEKKLQAASRITLRDFRDDRNLAILTI 119
Query: 112 EDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGK-YLVGGEIELLNELPNPFAKYTLR-- 168
+DIY +K + A VF D HP + + + K + +GG+IE +N+L N + LR
Sbjct: 120 DDIYRPDKTKEAKLVFGG-DPEHPAIVYLNNTVKEFYIGGKIEAVNKL-NHYDYVALRYT 177
Query: 169 PIETRVLFKEKGWKTVVAFQTRNVPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGDYKD 227
P E RV F + GW VVAFQTRN H H E +AA + + I+PV+G K GD
Sbjct: 178 PAELRVHFDKLGWSRVVAFQTRNPMHRAHRELTVRAARSRQANVLIHPVVGLTKPGDIDH 237
Query: 228 EVIIKAYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAG 287
++AY+ L Y P +AVL + MR GPREAI HAI+RKN GATHFIVGRDHAG
Sbjct: 238 FTRVRAYQALLPRY-PNGMAVLGLLGLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAG 296
Query: 288 VG------NYYGPYEAWDLFDEFPD-LGITPMFIREAFYCKKCGGMVNEKICPHDEKYHV 340
G ++YGPY+A +++ D LGI + + Y P K +
Sbjct: 297 PGSNSKGEDFYGPYDAQHAVEKYKDELGIEVVEFQMVTYLPDTDEYRPVDQVPAGVK-TL 355
Query: 341 RISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNP 376
ISGT+LR +R PE+ PEV +++R + P
Sbjct: 356 NISGTELRRR-LRSAHIPEWFSYPEVVKILRESNPP 390
>sp|P78937|MET3_SCHPO SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|1827504|dbj|BAA12186.1| (D83992)
putative sulfate adenylyltransferase, most similer to
pir :S55034 sulfate adenylyltransferase (61.3% identity
in 426 aa overlap) [Schizosaccharomyces pombe]
Length = 490
Score = 205 bits (516), Expect = 8e-52
Identities = 132/373 (35%), Positives = 203/373 (54%), Gaps = 28/373 (7%)
Query: 20 RERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDDTPWT 79
RE++ E PK+ + D+E I +G +SPL GF+ ++D+ +V++++RLS +
Sbjct: 17 REQLEQEATSLPKIVLSERXFCDVELILNGGFSPLDGFMNQKDYLNVVENLRLSTGEVFP 76
Query: 80 IPIVLDVEKPE---FEEGDAILLYHK---ETPIARMHVEDIYTYEKEEFALKVFKTKDAN 133
IPI D+ + + + GD + L +T IA + VED YT +K A KVF D
Sbjct: 77 IPITXDLNESQPDSLKAGDRVALIDPRDGQTVIAILTVEDKYTPDKANEAEKVFGANDRA 136
Query: 134 HPGVAKVYSM-GKYLVGGEIELLNELPN-PFAKYTLRPIETRVLFKEKGWKTVVAFQTRN 191
HP V ++ G VGG+++ + + + F +Y P + R F+ W VVAFQTRN
Sbjct: 137 HPAVDYLFGRAGNVYVGGKLQAVTPIRHFDFVEYRYSPAQLRSDFQRNNWNRVVAFQTRN 196
Query: 192 VPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDVAVLA 250
H H E +AA + I+PV+G K GD ++ YE + + Y PK A L+
Sbjct: 197 PMHRAHRELTVRAAKQHGARVLIHPVVGMTKPGDIDHFTRVRVYEAILQRY-PKGSAKLS 255
Query: 251 TVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVG------NYYGPYEAWDLFDEF 304
+ MR AGPREA+ HAI+RKN+GA+HFI+GRDHAG G ++YGPY+A L +++
Sbjct: 256 LLPLXMRMAGPREALWHAIIRKNYGASHFIIGRDHAGPGKNSQGEDFYGPYDAQYLVEQY 315
Query: 305 -PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYH-----VRISGTKLRNMIMRGEKPP 358
++GIT + + Y + +E I +K + ISGT+LR + G+ P
Sbjct: 316 AQEIGITIVPFQMMTY------LPDEDIYKPVDKVEPGTRTLNISGTELRRRLRVGDNIP 369
Query: 359 EYMMRPEVYEVIR 371
E+ PEV ++R
Sbjct: 370 EWFSYPEVVAILR 382
>sp|O66036|SAT_CHRVI SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|3132838|gb|AAC23622.1| (U84759)
sulfate adenylyltransferase [Allochromatium vinosum]
Length = 397
Score = 204 bits (514), Expect = 1e-51
Identities = 131/392 (33%), Positives = 199/392 (50%), Gaps = 26/392 (6%)
Query: 4 KPHGGKLIRR--IAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTRE 61
KP G +R + P R+ SE P V + A + + G +SPL GF+
Sbjct: 3 KPVGSDELRPRFVYDPEQHHRLSSEAESLPSVIVSSQAAGNAVMLGAGYFSPLDGFMNLA 62
Query: 62 DFESVLDHMRLSDDTPWTIPIVLDVEKPEFEEGDAILLYHKETP-----IARMHVEDIYT 116
D S M L+D + +P++ +E + G + +A M V +
Sbjct: 63 DALSSAQSMTLTDGRFFPVPLLCLLESADAIAGATRIALRDPNVEGNPVLAVMDVTAVEQ 122
Query: 117 YEKEEFAL---KVFKTKDANHPGVAKVYSMGKYLVGGEIELLN------ELPNPFAKYTL 167
+ AL +V+ T D HPGV S G+ + G I++LN + P+ F
Sbjct: 123 VSDAQMALMTEQVYGTSDPKHPGVETFNSQGRTAISGPIQVLNFSYFQTDFPDTFRT--- 179
Query: 168 RPIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFV--DGLFINPVLGRKKRGDY 225
+E R +E+GW+ +VAFQTRN H HE + K A+ V DG+ I+ +LG+ K GD
Sbjct: 180 -AVEIRHEIQERGWQKIVAFQTRNPMHRAHEELCKMAMEAVEADGVVIHMLLGQLKPGDI 238
Query: 226 KDEVIIKAYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDH 285
V A + E Y+P + ++ ++M YAGPREA+ HA R+N GATHFI+GRDH
Sbjct: 239 PAPVRDAAIRTMAELYFPPNTVMVTGYGFDMLYAGPREAVLHAYFRQNMGATHFIIGRDH 298
Query: 286 AGVGNYYGPYEAWDLFDEFPDLGITPMFIREA---FYCKKCGGMVNEKICP-HDEKYHVR 341
AGVG+YYGP++A +FD+ + + I A Y KK G +V + P H ++
Sbjct: 299 AGVGDYYGPFDAQTIFDDAVPTDVLAIEIFRADNTAYSKKLGRVVMMRDAPDHTPDDFIQ 358
Query: 342 ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
+SGT++R M+ +GE PP RPEV +++ +
Sbjct: 359 LSGTRVREMLGQGEAPPPEFSRPEVAQILMDY 390
>sp|P56862|MET3_ASPTE SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|6841069|gb|AAF28890.1|AF123267_2
(AF123267) sulfate adenylyltransferase [Aspergillus
terreus]
Length = 568
Score = 201 bits (507), Expect = 9e-51
Identities = 141/404 (34%), Positives = 212/404 (51%), Gaps = 48/404 (11%)
Query: 1 MVSKPHGGKLIRRIA--APRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFL 58
M + PHGG L +A APR + + +E P + + + DLE I +G +SPL+GF+
Sbjct: 1 MANSPHGGVLKDLLAGDAPR-HDELAAEAETLPAIVLSERQLCDLELIMNGGFSPLEGFM 59
Query: 59 TREDFESVLDHMRLSDDTPWTIPIVLD-----VEKPEFEEGDAILL--YHKETPIARMHV 111
T++DF+ V ++ RL+D +++PI LD + + G + L + + +A + +
Sbjct: 60 TQKDFDGVCENCRLADGHLFSMPITLDASQQVISDSNLKPGSRVTLRDFRDDRNLAILTI 119
Query: 112 ED-----IYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYT 166
E+ ++ + E A+K F TK + + +GG+IE +N+L N +
Sbjct: 120 EEKEAKLVFGGDPEHPAIKYFNTK------------VEDFYIGGKIEAVNKL-NHYDYVA 166
Query: 167 LR--PIETRVLFKEKGWKTVVAFQTRNVPHLGH-EYVQKAALTFVDGLFINPVLGRKKRG 223
LR P E RV F + GW VVAFQTRN H H E +AA + I+PV+G K G
Sbjct: 167 LRYSPAELRVHFDKLGWTRVVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPG 226
Query: 224 DYKDEVIIKAYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGR 283
D ++AY+ L Y P +AVL + MR GPREAI HAI+RKN GATHFIVGR
Sbjct: 227 DIDHFTRVRAYQALLPRY-PNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGR 285
Query: 284 DHAGVG------NYYGPYEAWDLFDEF-PDLGITPMFIREAFYCKKCGGMVNEKICPHDE 336
DHAG G +YGPY+A +++ +LGI + ++ Y ++ P DE
Sbjct: 286 DHAGPGKNSKGEEFYGPYDAQHAVEKYREELGIEVVEFQQVTYLPD-----TDEYKPKDE 340
Query: 337 K----YHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNP 376
+ ISGT+LRN + G PE+ PEV +++ +P
Sbjct: 341 VPAGIKTLDISGTELRNRLRTGAHIPEWFSYPEVVKILLRESSP 384
>sp|Q9Y872|MET3_CANAL SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|5566319|gb|AAD45374.1|AF164103_1
(AF164103) ATP sulfurylase [Candida albicans]
Length = 527
Score = 195 bits (491), Expect = 6e-49
Identities = 143/410 (34%), Positives = 215/410 (51%), Gaps = 44/410 (10%)
Query: 2 VSKPHGGKLIRRIA--APRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLT 59
+ PHGGKL + AP ++++L E P + + + DLE I +G +SPL GFL
Sbjct: 3 IPTPHGGKLRDLVIRDAP-LKQQLLQEAKTLPALTLTARQLCDLELILNGGFSPLTGFLN 61
Query: 60 REDFESVLDHMRLSD--------DTPWTIPIVLDVEKPEFEE---GDAILLY--HKETPI 106
+ED+ SV++ +RLS W IPI LDV++ ++ GD I+L ETP+
Sbjct: 62 QEDYNSVVNDLRLSSVKNESNGKGLLWPIPITLDVDETTSKKHSVGDRIVLIDLRDETPL 121
Query: 107 ARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKVY-SMGKYLVGGEIELLNELPNPFAKY 165
A + +E IY +K+ A KVF+ D HP + + G Y +GGE++ +N P +
Sbjct: 122 AILTIESIYKPDKKLEAKKVFRG-DPEHPANKYLLETAGDYYIGGELQGIN-YPKHYDYV 179
Query: 166 TLR--PIETRVLFKEKGW--KTVVAFQTRNVPHLGH-EYVQKAALTFVDG--LFINPVLG 218
R P E R F++ GW + +VAFQTRN H H E +AA D + I+PV+G
Sbjct: 180 DARKTPTELRQEFEKLGWAQENIVAFQTRNPMHRAHRELTIRAAQDIGDKAHILIHPVVG 239
Query: 219 RKKRGDYKDEVIIKAYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATH 278
K GD +K Y+ + + P +A L+ + MR G REA+ HA++R N+G H
Sbjct: 240 LTKPGDIDHHTRVKVYKQILTKF-PDGLATLSLLPLAMRMGGDREALWHALIRTNYGVDH 298
Query: 279 FIVGRDHAGVG------NYYGPYEAWDLFDEFPD-LGITPMFIREAFYCKKCGGMVNEKI 331
FIVGRDHAG G ++YGPY+A +L ++ D L I + R Y ++
Sbjct: 299 FIVGRDHAGPGKNSQGVDFYGPYDAQELLAKYDDELNIKIVPFRMVTYLPD-----EDRY 353
Query: 332 CPHD-----EKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNP 376
P D + ISGT+LRN + G++ P + PEV +++R + P
Sbjct: 354 APIDTIDVKKVRTANISGTELRNKLKTGDEIPSWFSYPEVVKILRETNPP 403
>pir||T08094 probable sulfate adenylyltransferase (EC 2.7.7.4) ATS1 -
Chlamydomonas reinhardtii >gi|1336213|gb|AAB01234.1|
(U57088) ATP sulfurylase Ats1 [Chlamydomonas
reinhardtii]
Length = 437
Score = 149 bits (372), Expect = 6e-35
Identities = 111/346 (32%), Positives = 169/346 (48%), Gaps = 19/346 (5%)
Query: 39 RAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDDTPWTIPIVLDVEKPEFEEGDAIL 98
R D+E + G +SPL+GF+ + +++SV+ +MR+++ + +PIVLD + + GD +L
Sbjct: 90 RTCDVELLTVGAFSPLEGFMNKAEYDSVVANMRMTNGLLFGLPIVLDTDSEDIVPGDKLL 149
Query: 99 LYHKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKV-YSMGKYLVGGEIELLNE 157
L ++ +A M V+ +T K LK + T HP V V GKY +GG I+ L
Sbjct: 150 LTYQGQDLAVMTVDSKFTPNKPLECLKCYGTSSLEHPAVQMVAMERGKYYIGGPIKGLAL 209
Query: 158 LPNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGH-EYVQKAALTFVDG----LF 212
F P + R + V+AFQ RN H H E +A V
Sbjct: 210 PTRVFP--CASPADVRSTLPAN--QDVLAFQCRNPIHKAHYELFIRALDRNVRNPGAVCL 265
Query: 213 INPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRK 272
++P G + D V + YEVL E + A + Y M AGPREAI H I+RK
Sbjct: 266 VHPTCGPTQDDDIPGVVRFRTYEVLKEETKNPRLR-WAYLPYSMHMAGPREAIQHMIIRK 324
Query: 273 NFGATHFIVGRDHAGV------GNYYGPYEAWDLFDEF-PDLGITPMFIREAFYCKKCGG 325
N+G THFI+GRD AG ++Y Y+A DL ++ +L + + Y ++ G
Sbjct: 325 NYGCTHFIIGRDMAGCKSSISGQDFYRAYDAQDLANKHAAELNMQTVASLNIAYTEE-KG 383
Query: 326 MVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIR 371
V I + + + +SGTK R M+ G+ PE+ V V+R
Sbjct: 384 YVTADIAKAENLHVLNLSGTKFRQMLRAGDDIPEWFAFKSVVAVLR 429
>dbj|BAB00629.1| (AB036852) ATP sulfurylase/APS kinase [Ciona intestinalis]
Length = 618
Score = 127 bits (316), Expect = 2e-28
Identities = 118/376 (31%), Positives = 167/376 (44%), Gaps = 40/376 (10%)
Query: 25 SEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDDTP--WTIPI 82
+E P + I L+ ++ G +PL GF+ +F L + P ++PI
Sbjct: 242 AEAEALPSIDITKLDMQWLQVLSEGWATPLTGFMREREFLQCQHFGCLLKNEPINQSVPI 301
Query: 83 VLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAK 139
VL V +K E +AI L + A +H + Y + KEE + + T + HP +
Sbjct: 302 VLPVHSADKERVENAEAIALKYDGQVKAILHKPEFYPHLKEERCSRQWGTSNKGHPHIKM 361
Query: 140 VYSMGKYLVGGEIELLNELP--NPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGH 197
+ G +L GG+IE+L + + KY + P+E R FK V AFQ RN H GH
Sbjct: 362 IMESGDWLCGGDIEVLERITWGDGLDKYRMTPLELRAKFKSMNADAVFAFQLRNPVHNGH 421
Query: 198 E-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVIIKAYE-VLFEHYYPKDVAVL 249
+Q V+ F NPVL G K D +V +K + VL E V+
Sbjct: 422 ALLMQDTKRKLVERGFKNPVLLLHPLGGWTKSDDVPLDVRMKQHSAVLEEGVLDPQSTVV 481
Query: 250 ATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDLFDE------ 303
A M YAGP E HA R GA +IVGRD AG+ + P E DL+D
Sbjct: 482 AIFPAPMMYAGPTEVQWHAKARLATGANFYIVGRDPAGMPH---PDEKRDLYDHSHGRKV 538
Query: 304 ---FPDLG---ITPMFIREAFYCKKCGGMVNEKICPHDEKYHVR---ISGTKLRNMIMRG 354
P LG I P R A Y K +K+ +D + H ISGT++R G
Sbjct: 539 LTMAPGLGRFEIVP--FRVAAYNK-----TTKKMDFYDPENHDNFEFISGTRMRRAAREG 591
Query: 355 EKPPEYMMRPEVYEVI 370
E PE M P+ ++V+
Sbjct: 592 ESLPEGFMAPKAWDVL 607
>sp|Q54506|SAT_RIFPS SULFATE ADENYLYLTRANSFERASE (SULFATE ADENYLATE TRANSFERASE) (SAT)
(ATP-SULFURYLASE) >gi|2147136|pir||I40176 ATP
sulfurylase - unidentified bacterium
>gi|537493|gb|AAA72419.1| (L26897) ATP sulfurylase
[Sulfur-oxidizing endosymbiont of Riftia pachyptila]
Length = 437
Score = 127 bits (315), Expect = 3e-28
Identities = 89/283 (31%), Positives = 132/283 (46%), Gaps = 20/283 (7%)
Query: 17 PRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDDT 76
P ++ E P V I + G +SP GF+ D + M LSD +
Sbjct: 18 PEEHHKLSHEAESLPSVVISSQGPRVSSMMGAGYFSPA-GFMNVADAMGAAEKMTLSDGS 76
Query: 77 PWTIPIVLDVEKPEFEEGDAILLYHKETP----IARMHVEDIYTYEKEEFAL---KVFKT 129
+ L + I L +A M +E I E+ A+ KV++T
Sbjct: 77 SSCSVLCLLENTDAIGDAKRIALRDPNVEGNPVLAVMDIEAIEEVSDEQMAVMTDKVYRT 136
Query: 130 KDANHPGVAKVYSMGKYLVGGEIELLN------ELPNPFAKYTLRPIETRVLFKEKGWKT 183
D +H GV S G+ V G I++LN + P+ F +E R KE GW
Sbjct: 137 TDMDHIGVKTFNSQGRVAVSGPIQVLNFSYFQADFPDTFRT----AVEIRNEIKEHGWSK 192
Query: 184 VVAFQTRNVPHLGHEYVQKAALTFVD--GLFINPVLGRKKRGDYKDEVIIKAYEVLFEHY 241
VVAFQTRN H HE + + + +D G+ ++ +LG+ K+GD V A + E Y
Sbjct: 193 VVAFQTRNPMHRAHEELCRMPMESLDADGVVVHMLLGKLKKGDIPAPVRDAAIRTMAEVY 252
Query: 242 YPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRD 284
+P + ++ ++M YAGPREA+ HA R+N GATHFI+GR+
Sbjct: 253 FPPNTVMVTGYGFDMLYAGPREAVLHAYFRQNMGATHFIIGRE 295
>pir||T24918 3'-phosphoadenosine-5'-phosphosulfate synthetase - Caenorhabditis
elegans >gi|3879879|emb|CAA93098.1| (Z68880) predicted
using Genefinder~Similarity to Potato Sulphate
adenyltransferase (PIR Acc. No. S44079), contains
similarity to Pfam domain: PF01583 (Adenylylsulfate
kinase), Score=406.9, E-value=6.3e-119, N=1; PF01747
(ATP-sulfurylase), Score=665.2,>
Length = 652
Score = 126 bits (313), Expect = 5e-28
Identities = 110/397 (27%), Positives = 164/397 (40%), Gaps = 44/397 (11%)
Query: 19 TRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVL----------- 67
T +L E P V++ L+ +A G +PL GF+ + +
Sbjct: 250 TVAELLKESQNLPTVELTKVDLQWLQVLAEGWATPLSGFMRERQYLQSMHFGQLLDLKHK 309
Query: 68 ------------DHMRLSDDTPWTIPIVLDVE---KPEFEEGDAILLYHKETPIARMHVE 112
D + DD +IPIVL + K E I L + A +
Sbjct: 310 VAFVGEKSDDKEDSWPMMDDINQSIPIVLPISDDVKKGLEGVTRIALKYNGQVYAILSDP 369
Query: 113 DIYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELP--NPFAKYTLRPI 170
+I+ + K+E + F T D HP VA+V G +L+GG++ ++ ++ + KY P
Sbjct: 370 EIFEHRKDERVCRQFGTNDPRHPAVAQVLESGNWLLGGDVAVVQKIQFNDGLDKYRKTPN 429
Query: 171 ETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGLFINPVL------GRKKRGD 224
E R +F EK V AFQ RN H GH + + + NP+L G K D
Sbjct: 430 ELRAIFAEKNADAVFAFQLRNPIHNGHALLMRDTREKLLAEHKNPILLLHPLGGWTKDDD 489
Query: 225 YKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGR 283
++ IK +E V+ E + VL+ M YAGP E HA R G H+IVGR
Sbjct: 490 VPLDIRIKQHEAVIAERVLDPEWTVLSIFPSPMMYAGPTEVQWHARSRIAAGIQHYIVGR 549
Query: 284 DHAGVGNYYGPYEAWDLFDEFPDLGITP-------MFIREAFYCKKCGGMVNEKICPHDE 336
D AG+ P ++ L + P + R A Y K M ++
Sbjct: 550 DPAGIQKPGSPDALYETTHGAKVLSMAPGLSALHILPFRVAAYDKTAKKMSFFDTSRKED 609
Query: 337 KYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
ISGTK+R + G+ PPE M P +EV+ +
Sbjct: 610 --FENISGTKMRGLARNGDTPPEGFMAPTAWEVLAGY 644
>gb|AAF19185.1|AF198964_1 (AF198964) ATP sulfurylase [Arabidopsis thaliana]
Length = 463
Score = 124 bits (309), Expect = 1e-27
Identities = 119/400 (29%), Positives = 183/400 (45%), Gaps = 44/400 (11%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTRE 61
P GGKL+ I R E + P+V++ AIDL+ ++ G SPL GF+
Sbjct: 53 PDGGKLVELIVEEPKRREKKHEAADLPRVELT---AIDLQWMHVLSEGWASPLGGFMRES 109
Query: 62 DFESVL--DHMRLSDDTP--WTIPIVL---DVEKPEFEEGDAILLYHKE-TPIARMHVED 113
+F L + +RL D + ++PIVL D +K E + L++ + P+A + +
Sbjct: 110 EFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIE 169
Query: 114 IYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRPI 170
IY + KEE + + T P V + + + G +L+GG++E+L + + ++ L P
Sbjct: 170 IYKHPKEERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPA 229
Query: 171 ETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGL-------FINPVLGRKKRG 223
E R +++ V AFQ RN H GH + + + F + V G K
Sbjct: 230 ELRKELEKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYQKPYSFASSVSGFTKAD 289
Query: 224 DYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVG 282
D + +K +E VL + + V++ M YAGP E HA R N GA +IVG
Sbjct: 290 DVPLDWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVG 349
Query: 283 RDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVNEK 330
RD AG+G+ P E DL+D L I P R A Y K G M
Sbjct: 350 RDPAGMGH---PVEKRDLYDADHGKKVLSMAPGLERLNILP--FRVAAYDKTQGKMA--F 402
Query: 331 ICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 403 FDPSRPQDFLFISGTKMRTLAKNNENPPDGFMCPGGWKVL 442
>emb|CAA11417.1| (AJ223499) ATP sulfurylase [Brassica juncea]
Length = 461
Score = 124 bits (308), Expect = 2e-27
Identities = 123/401 (30%), Positives = 189/401 (46%), Gaps = 46/401 (11%)
Query: 5 PHGGKLIRRIA-APRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTR 60
P GGKL+ + PR RE+ E E P+V++ AID++ ++ G SPL GF+
Sbjct: 51 PDGGKLVELVVDEPRRREK-KHEAGELPRVELT---AIDMQWMHVLSEGWASPLGGFMRE 106
Query: 61 EDFESVL--DHMRLSDDTP--WTIPIVL---DVEKPEFEEGDAILLYHKE-TPIARMHVE 112
+F L + +RL D + ++PIVL D +K E + L + P+A +
Sbjct: 107 SEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKAGIGESKRVALVDSDGNPVAILTDI 166
Query: 113 DIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRP 169
+IY + KEE + + T P V + + + G +L+GG++E+L + + ++ L P
Sbjct: 167 EIYKHPKEERIARTWGTTAPGLPYVEEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSP 226
Query: 170 IETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVLGRKKRGDYK-- 226
E R +++G V AFQ RN H GH + ++ + NP+L G Y
Sbjct: 227 AELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYTKA 286
Query: 227 DEVII----KAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIV 281
D+V + K +E VL + + V++ M YAGP E HA R N GA +IV
Sbjct: 287 DDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIV 346
Query: 282 GRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVNE 329
GRD AG+G+ P E DL+D L I P + A Y K G M
Sbjct: 347 GRDPAGMGH---PVEKRDLYDADHGKKVLSMAPGLERLNILP--FKVAAYDKTQGKMA-- 399
Query: 330 KICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 400 FFDPSRPQDFLFISGTKMRTLAKNKENPPDGFMCPGGWQVL 440
>gb|AAA21570.1| (U05218) ATP sulfurylase [Arabidopsis thaliana]
Length = 463
Score = 123 bits (307), Expect = 2e-27
Identities = 119/400 (29%), Positives = 183/400 (45%), Gaps = 44/400 (11%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTRE 61
P GGKL+ I R E + P+V++ AIDL+ ++ G SPL GF+
Sbjct: 53 PDGGKLVGLIVEEPKRREKKHEAADLPRVELT---AIDLQWMHVLSEGWASPLGGFMRES 109
Query: 62 DFESVL--DHMRLSDDTP--WTIPIVL---DVEKPEFEEGDAILLYHKE-TPIARMHVED 113
+F L + +RL D + ++PIVL D +K E + L++ + P+A + +
Sbjct: 110 EFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIE 169
Query: 114 IYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRPI 170
IY + KEE + + T P V + + + G +L+GG++E+L + + ++ L P
Sbjct: 170 IYKHPKEERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPA 229
Query: 171 ETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGL-------FINPVLGRKKRG 223
E R +++ V AFQ RN H GH + + + F + V G K
Sbjct: 230 ELRKELEKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYQKPYSFASSVSGFTKAD 289
Query: 224 DYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVG 282
D + +K +E VL + + V++ M YAGP E HA R N GA +IVG
Sbjct: 290 DVPLDWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVG 349
Query: 283 RDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVNEK 330
RD AG+G+ P E DL+D L I P R A Y K G M
Sbjct: 350 RDPAGMGH---PVEKRDLYDADHGKKVLSMAPGLERLNILP--FRVAAYDKTQGKMA--F 402
Query: 331 ICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 403 FDPSRPQDFLFISGTKMRTLAKNNENPPDGFMCPGGWKVL 442
>pir||S44267 sulfate adenylyltransferase (EC 2.7.7.4) met3-2 - potato
>gi|2130000|pir||S67498 sulfate adenylyltransferase (EC
2.7.7.4) (clone StMet3-2) - potato
>gi|479090|emb|CAA55655.1| (X79053) sulfate
adenylyltransferase [Solanum tuberosum]
Length = 463
Score = 123 bits (306), Expect = 3e-27
Identities = 115/398 (28%), Positives = 180/398 (44%), Gaps = 38/398 (9%)
Query: 4 KPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDF 63
+P GGKL+ I R+ + P++++ + ++ G SPLKGF+ +F
Sbjct: 52 EPDGGKLVELIVEEPQRDLKRRQALSLPQIKLSKIDIQWVHVLSEGWASPLKGFMRESEF 111
Query: 64 ESVL--DHMRLSDDTP--WTIPIVL---DVEKPEFEEGDAILLYH-KETPIARMHVEDIY 115
L + +RL D + ++PIVL D K + ++ L K+ PIA ++ +IY
Sbjct: 112 LQTLHFNSLRLGDGSVVNMSVPIVLAIDDSNKNNIGDSSSVALVDDKDNPIAILNDIEIY 171
Query: 116 TYEKEEFALKVFKTKDANHPGVAKVYS-MGKYLVGGEIELLNELP--NPFAKYTLRPIET 172
+ KEE + + T P + + G +L+GG++E++ + + ++ L P E
Sbjct: 172 KHNKEERTARTWGTTAPGLPYAEQAITHAGNWLIGGDLEVIEPIKYHDGLDRFRLSPAEL 231
Query: 173 RVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------GRKKRGDY 225
R F + V AFQ RN H GH + ++ + NPVL G K D
Sbjct: 232 RDEFTRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGYTKADDV 291
Query: 226 KDEVIIKAYEVLFEH-YYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRD 284
E +K +E++ E + V++ M YAGP E HA R N GA +IVGRD
Sbjct: 292 PLEWRMKQHEMVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRD 351
Query: 285 HAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVNEKIC 332
AG+G+ P E DL+D L I P + A Y K GM
Sbjct: 352 PAGMGH---PLEKRDLYDADHGKKVLSMAPGLERLNILP--FKVAAYDKTQNGMA--FFD 404
Query: 333 PHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 405 PSRPQDFLFISGTKMRALAKNKENPPDGFMCPGGWKVL 442
>gb|AAD26634.1| (AF110407) ATP sulfurylase precursor [Arabidopsis thaliana]
>gi|4803653|emb|CAB42640.1| (AJ012586) sulfate
adenylyltransferase [Arabidopsis thaliana]
>gi|10177947|dbj|BAB11306.1| (AB026651) ATP sulfurylase
precursor [Arabidopsis thaliana]
Length = 469
Score = 122 bits (304), Expect = 5e-27
Identities = 121/407 (29%), Positives = 187/407 (45%), Gaps = 55/407 (13%)
Query: 4 KPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENI---AHGVYSPLKGFLTR 60
+P GGKL+ + +R R++ + E +I R +DLE + + G SPLKGF+ +
Sbjct: 55 EPDGGKLMN-LVVEESRRRVMKHEAETVPARIKLNR-VDLEWVHVLSEGWASPLKGFMRQ 112
Query: 61 EDFESVL--DHMRLSDDTP--WTIPIVLDVEKPE-FEEGDA---ILLYHKETPIARMHVE 112
+F L + RL D + ++PIVL ++ + F GD+ L+ PIA ++
Sbjct: 113 SEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIAILNDI 172
Query: 113 DIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRP 169
+IY + KEE + + T P + + G +L+GG++++L + + ++ L P
Sbjct: 173 EIYKHPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDRFRLSP 232
Query: 170 IETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDG-------------LFINPV 216
+ R F +G V AFQ RN H GH AL D L +NP+
Sbjct: 233 SQLREEFIRRGADAVFAFQLRNPVHNGH------ALLMTDTRRRLLEMGYKNPVLLLNPL 286
Query: 217 LGRKKRGDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFG 275
G K D ++ +E VL + + V++ M YAGP E HA R N G
Sbjct: 287 GGFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAG 346
Query: 276 ATHFIVGRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKC 323
A +IVGRD AG+G+ P E DL+D L I P + A Y K
Sbjct: 347 ANFYIVGRDPAGMGH---PTEKRDLYDADHGKKVLSMAPGLERLNILP--FKVAAYDKTQ 401
Query: 324 GGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
G M P + + ISGTK+R + + E PP+ M P ++V+
Sbjct: 402 GKMA--FFDPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVL 446
>pir||S68201 sulfate adenylyltransferase (EC 2.7.7.4) precursor (clone APS3) -
Arabidopsis thaliana >gi|459144|gb|AAA92350.1| (U06275)
ATP sulfurylase [Arabidopsis thaliana]
Length = 465
Score = 122 bits (303), Expect = 7e-27
Identities = 123/402 (30%), Positives = 187/402 (45%), Gaps = 46/402 (11%)
Query: 4 KPHGGKLIRRIAA-PRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLT 59
+P GGKL+ + PR RE+ E + P+V++ AIDL+ ++ G SPL+GF+
Sbjct: 54 EPDGGKLVDLVVPEPRRREK-KHEAADLPRVRLT---AIDLQWMHVLSEGWASPLRGFMR 109
Query: 60 REDFESVLDHMRLSDD----TPWTIPIVL---DVEKPEFEEGDAILLYHKE-TPIARMHV 111
+F L L+ D ++PIVL D +K E + L + PIA ++
Sbjct: 110 ESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILND 169
Query: 112 EDIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLR 168
+IY + KEE + + T P V + + + G +L+GG++E+L + + ++ L
Sbjct: 170 IEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLS 229
Query: 169 PIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------GRKK 221
P E R +++G V AFQ RN H GH + ++ + NP+L G K
Sbjct: 230 PFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTK 289
Query: 222 RGDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFI 280
D +K +E VL + + V++ M YAGP E HA R N GA +I
Sbjct: 290 ADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYI 349
Query: 281 VGRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVN 328
VGRD AG+G+ P E DL+D L I P R A Y K G M
Sbjct: 350 VGRDPAGMGH---PVEKRDLYDADHGKKVLSMAPGLERLNILP--FRVAAYDKTQGKMA- 403
Query: 329 EKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 404 -FFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVL 444
>gb|AAB09473.1| (U59738) ATP sulfurylase [Arabidopsis thaliana]
Length = 465
Score = 122 bits (303), Expect = 7e-27
Identities = 123/402 (30%), Positives = 187/402 (45%), Gaps = 46/402 (11%)
Query: 4 KPHGGKLIRRIAA-PRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLT 59
+P GGKL+ + PR RE+ E + P+V++ AIDL+ ++ G SPL+GF+
Sbjct: 54 EPDGGKLVDLVVPEPRRREK-KHEAADLPRVRLT---AIDLQWMHVLSEGWASPLRGFMR 109
Query: 60 REDFESVLDHMRLSDD----TPWTIPIVL---DVEKPEFEEGDAILLYHKE-TPIARMHV 111
+F L L+ D ++PIVL D +K E + L + PIA ++
Sbjct: 110 ESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILND 169
Query: 112 EDIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLR 168
+IY + KEE + + T P V + + + G +L+GG++E+L + + ++ L
Sbjct: 170 IEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLS 229
Query: 169 PIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------GRKK 221
P E R +++G V AFQ RN H GH + ++ + NP+L G K
Sbjct: 230 PFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTK 289
Query: 222 RGDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFI 280
D +K +E VL + + V++ M YAGP E HA R N GA +I
Sbjct: 290 ADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYI 349
Query: 281 VGRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVN 328
VGRD AG+G+ P E DL+D L I P R A Y K G M
Sbjct: 350 VGRDPAGMGH---PVEKRDLYDADHGKKVLSMAPGLERLNILP--FRVAAYDKTQGKMA- 403
Query: 329 EKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 404 -FFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVL 444
>pir||E71409 sulfate adenylyltransferase (EC 2.7.7.4) precursor (clone APS3) -
Arabidopsis thaliana >gi|2244824|emb|CAB10247.1|
(Z97336) ATP-sulfurylase [Arabidopsis thaliana]
>gi|7268174|emb|CAB78510.1| (AL161539) ATP-sulfurylase
[Arabidopsis thaliana]
Length = 465
Score = 122 bits (303), Expect = 7e-27
Identities = 123/402 (30%), Positives = 187/402 (45%), Gaps = 46/402 (11%)
Query: 4 KPHGGKLIRRIAA-PRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLT 59
+P GGKL+ + PR RE+ E + P+V++ AIDL+ ++ G SPL+GF+
Sbjct: 54 EPDGGKLVDLVVPEPRRREK-KHEAADLPRVRLT---AIDLQWMHVLSEGWASPLRGFMR 109
Query: 60 REDFESVLDHMRLSDD----TPWTIPIVL---DVEKPEFEEGDAILLYHKE-TPIARMHV 111
+F L L+ D ++PIVL D +K E + L + PIA ++
Sbjct: 110 ESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSDDNPIAILND 169
Query: 112 EDIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLR 168
+IY + KEE + + T P V + + + G +L+GG++E+L + + ++ L
Sbjct: 170 IEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYNDGLDRFRLS 229
Query: 169 PIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------GRKK 221
P E R +++G V AFQ RN H GH + ++ + NP+L G K
Sbjct: 230 PFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTK 289
Query: 222 RGDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFI 280
D +K +E VL + + V++ M YAGP E HA R N GA +I
Sbjct: 290 ADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGANFYI 349
Query: 281 VGRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVN 328
VGRD AG+G+ P E DL+D L I P R A Y K G M
Sbjct: 350 VGRDPAGMGH---PVEKRDLYDADHGKKVLSMAPGLERLNILP--FRVAAYDKTQGKMA- 403
Query: 329 EKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 404 -FFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVL 444
>gb|AAF20366.1|AF150754_1 (AF150754) 3'phosphoadenosine 5'-phosphosulfate synthase 2b isoform
[Homo sapiens]
Length = 619
Score = 122 bits (303), Expect = 7e-27
Identities = 116/395 (29%), Positives = 179/395 (44%), Gaps = 46/395 (11%)
Query: 23 ILSEQHEY--PKVQIDHGRA------------IDLENI---AHGVYSPLKGFLTRED--- 62
I+ + HE P+ ++DH RA +DL+ + + G +PLKGF+ ++
Sbjct: 220 IIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQ 279
Query: 63 ---FESVLDHMRLSDDT-PWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIY 115
F+++LD M L D +IPIVL V +K E +L H +A + + Y
Sbjct: 280 VMHFDALLDGMALPDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEFY 339
Query: 116 TYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETR 173
+ KEE +V+ T HP + V G +LVGG++++L ++ + +Y L P+E +
Sbjct: 340 EHRKEERCSRVWGTTCTKHPHIKMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELK 399
Query: 174 VLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYK 226
KE V AFQ RN H GH +Q T ++ + +PVL G K D
Sbjct: 400 QKCKEMNADAVFAFQLRNPVHNGHALLMQDTRRTVLERGYKHPVLLLHPLGGWTKDDDVP 459
Query: 227 DEVIIKAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDH 285
+ +K + VL E ++A M YAGP E H R GA +IVGRD
Sbjct: 460 LDWRMKQHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDP 519
Query: 286 AGV------GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKY 338
AG+ + Y P + P L + + R A Y K M H+E
Sbjct: 520 AGMPHPETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNE-- 577
Query: 339 HVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
ISGT++R + GE PP+ M P+ ++V+ +
Sbjct: 578 FDFISGTRMRKLAREGENPPDGFMAPKAWKVLTDY 612
>gb|AAD38423.1|AF074331_1 (AF074331) PAPS synthetase-2 [Homo sapiens]
Length = 614
Score = 122 bits (302), Expect = 9e-27
Identities = 113/390 (28%), Positives = 176/390 (44%), Gaps = 41/390 (10%)
Query: 23 ILSEQHEY--PKVQIDHGRA------------IDLENI---AHGVYSPLKGFLTREDFES 65
I+ + HE P+ ++DH RA +DL+ + + G +PLKGF+ +++
Sbjct: 220 IIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQ 279
Query: 66 VLDHMRLSDD--TPWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKE 120
V+ L DD +IPIVL V +K E +L H +A + + Y + KE
Sbjct: 280 VMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEFYEHRKE 339
Query: 121 EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKE 178
E +V+ T HP + V G +LVGG++++L ++ + +Y L P+E + KE
Sbjct: 340 ERCSRVWGTTCTKHPHIKMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKE 399
Query: 179 KGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVII 231
V AFQ RN H GH +Q ++ + +PVL G K D + +
Sbjct: 400 MNADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRM 459
Query: 232 KAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV-- 288
K + VL E ++A M YAGP E H R GA +IVGRD AG+
Sbjct: 460 KQHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPH 519
Query: 289 ----GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRIS 343
+ Y P + P L + + R A Y K M + H+E IS
Sbjct: 520 PETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDLARHNE--FDFIS 577
Query: 344 GTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
GT++R + GE PP+ M P+ ++V+ +
Sbjct: 578 GTRMRKLAREGENPPDGFMAPKAWKVLTDY 607
>pir||T08594 probable sulfate adenylyltransferase (EC 2.7.7.4) - rape
>gi|1527219|gb|AAB53100.1| (U68218) ATP sulphurylase
[Brassica napus]
Length = 459
Score = 121 bits (300), Expect = 2e-26
Identities = 119/401 (29%), Positives = 183/401 (44%), Gaps = 45/401 (11%)
Query: 5 PHGGKLIRRIA-APRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTR 60
P GGKL+ I PR RE+ E P+V++ AID++ ++ G SPL GF+
Sbjct: 48 PDGGKLVELIVDEPRRREKKHEAATELPRVELT---AIDMQWMHVLSEGWASPLGGFMRE 104
Query: 61 EDFESVL--DHMRLSDDTP--WTIPIVLDVE---KPEFEEGDAILLYHKE-TPIARMHVE 112
+F L + +RL D + ++PIVL ++ K E + L + P+A +
Sbjct: 105 SEFLQTLHFNSLRLDDGSVVNMSVPIVLPIDDEPKASIGESKRVALVDSDGNPVAILTDI 164
Query: 113 DIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRP 169
+IY + KEE + + T P V + + + G +L+GG++E+L + + ++ L P
Sbjct: 165 EIYKHPKEERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSP 224
Query: 170 IETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDGL-------FINPVLGRKKR 222
E R +++G V AFQ RN H GH + + + F + V G K
Sbjct: 225 AELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYQEPYPFASSVRGYTKA 284
Query: 223 GDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIV 281
D +K +E VL + + V++ M YAGP E HA R N GA +IV
Sbjct: 285 DDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIV 344
Query: 282 GRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVNE 329
GRD AG+ + P E DL+D L I P + A Y K G M
Sbjct: 345 GRDPAGIRH---PVEKRDLYDADHGKKVLSMAPGLERLNILP--FKVAAYDKTQGKMA-- 397
Query: 330 KICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 398 FFDPSRPQDFLFISGTKMRTLAKNKENPPDGFMCPGGWQVL 438
>gb|AAF49102.1| (AE003515) Paps gene product [Drosophila melanogaster]
Length = 635
Score = 121 bits (300), Expect = 2e-26
Identities = 109/382 (28%), Positives = 165/382 (42%), Gaps = 39/382 (10%)
Query: 27 QHEYPKVQIDHGRAIDLENI---AHGVYSPLKGFLTREDFESVLDHMRLSDDTPWT---- 79
+HE +Q ++L+ + A G PL+GF+ +++ L L +
Sbjct: 251 RHEAESLQAIEISTVELQWVQVLAEGWAYPLRGFMREDEYLQTLHFNTLQSGMDGSYREN 310
Query: 80 --IPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKEEFALKVFKTKDANH 134
+PIVL +K + ++ L ++ +A + + Y KEE + F T + NH
Sbjct: 311 HSVPIVLSATQADKDRLDGCSSLTLKYQGKAVAILRRPEFYFQRKEERLARQFGTSNPNH 370
Query: 135 PGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNV 192
P +VY G YLVGG++ ++ + + +Y L P E R FKE + AFQ RN
Sbjct: 371 PYSKQVYESGDYLVGGDLAVIERIRWEDGLDQYRLTPNELRRRFKELNADAIFAFQLRNP 430
Query: 193 PHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVIIKAYE-VLFEHYYPK 244
H GH +Q ++ F PVL G K D +V +K ++ VL +
Sbjct: 431 IHNGHALLMQDTRRQLLERGFKQPVLLLHPLGGWTKDDDVPLDVRMKQHQAVLDAGVLRR 490
Query: 245 DVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV----------GNYYGP 294
+ VLA M YAGP E HA R N GA +IVGRD AG+ GN Y
Sbjct: 491 EDTVLAIFPSPMMYAGPTEVQWHAKARMNAGANFYIVGRDPAGMPHPAKETYPDGNLYDA 550
Query: 295 ---YEAWDLFDEFPDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMI 351
+ + I P R A Y K M + P + ISGTK+R +
Sbjct: 551 THGARVLKMAQGLDSMEILP--FRVAAYDKSASRMAFFE--PKRKDEFEFISGTKMRTLA 606
Query: 352 MRGEKPPEYMMRPEVYEVIRSF 373
G PP+ M PE + ++ ++
Sbjct: 607 KTGASPPDGFMEPEAWRILATY 628
>emb|CAA73368.1| (Y12861) bifunctional ATP sulfurylase/APS kinase [Drosophila
melanogaster]
Length = 629
Score = 120 bits (298), Expect = 3e-26
Identities = 108/381 (28%), Positives = 164/381 (42%), Gaps = 38/381 (9%)
Query: 27 QHEYPKVQIDHGRAIDLE--NIAHGVYSPLKGFLTREDFESVLDHMRLSDDTPWT----- 79
+HE +Q ++L+ + G PL+GF+ +++ L L +
Sbjct: 246 RHEAESLQAIEISTVELQWVQVLAGWAYPLRGFMREDEYLQTLHFNTLQSGMDGSYRENH 305
Query: 80 -IPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKEEFALKVFKTKDANHP 135
+PIVL +K + ++ L ++ +A + + Y KEE + F T + NHP
Sbjct: 306 SVPIVLSATQADKDRLDGCSSLTLKYQGKAVAILRRPEFYFQRKEERLARQFGTSNPNHP 365
Query: 136 GVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVP 193
+VY G YLVGG++ ++ + + +Y L P E R FKE + AFQ RN
Sbjct: 366 YSKQVYESGDYLVGGDLAVIERIRWEDGLDQYRLTPNELRRRFKELNADAIFAFQLRNPI 425
Query: 194 HLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVIIKAYE-VLFEHYYPKD 245
H GH +Q ++ F PVL G K D +V +K ++ VL ++
Sbjct: 426 HNGHALLMQDTRRQLLERGFKQPVLLLHPLGGWTKDDDVPLDVRMKQHQAVLDAGVLRRE 485
Query: 246 VAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV----------GNYYGP- 294
VLA M YAGP E HA R N GA +IVGRD AG+ GN Y
Sbjct: 486 DTVLAIFPSPMMYAGPTEVQWHAKARMNAGANFYIVGRDPAGMPHPAKETYPDGNLYDAT 545
Query: 295 --YEAWDLFDEFPDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIM 352
+ + I P R A Y K M + P + ISGTK+R +
Sbjct: 546 HGARVLKMAQGLDSMEILP--FRVAAYDKSASRMAFFE--PKRKDEFEFISGTKMRTLAK 601
Query: 353 RGEKPPEYMMRPEVYEVIRSF 373
G PP+ M PE + ++ ++
Sbjct: 602 TGASPPDGFMEPEAWRILATY 622
>gi|4758880 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 2 [Homo
sapiens] >gi|6225865|sp|O95340|PPS2_HUMAN BIFUNCTIONAL
3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHETHASE 2
(PAPS SYNTHETHASE 2) (PAPSS 2) (SULFURYLASE KINASE 2)
(SK2) (SK 2) [INCLUDES: SULFATE ADENYLYLTRANSFERASE
(SULFATE ADENYLATE TRANSFERASE) (SAT) (ATP-SULFURYLASE);
ADENYLYLSULFATE KINASE (> >gi|3769610|gb|AAC64583.1|
(AF091242) ATP sulfurylase/APS kinase 2 [Homo sapiens]
Length = 614
Score = 120 bits (298), Expect = 3e-26
Identities = 113/390 (28%), Positives = 175/390 (43%), Gaps = 41/390 (10%)
Query: 23 ILSEQHEY--PKVQIDHGRA------------IDLENI---AHGVYSPLKGFLTREDFES 65
I+ + HE P+ ++DH RA +DL+ + + G +PLKGF+ +++
Sbjct: 220 IIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQ 279
Query: 66 VLDHMRLSDD--TPWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKE 120
V+ L DD +IPIVL V +K E +L H +A + + Y + KE
Sbjct: 280 VMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEFYEHRKE 339
Query: 121 EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKE 178
E +V+ T HP + V G +LVGG++++L ++ + +Y L P+E + KE
Sbjct: 340 ERCSRVWGTTCTKHPHIKMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKE 399
Query: 179 KGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVII 231
V AFQ RN H GH +Q ++ + +PVL G K D + +
Sbjct: 400 MNADAVFAFQLRNPVHNGHALLMQDTCRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRM 459
Query: 232 KAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV-- 288
K + VL E ++A M YAGP E H R GA +IVGRD AG+
Sbjct: 460 KQHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPH 519
Query: 289 ----GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRIS 343
+ Y P + P L + + R A Y K M H+E IS
Sbjct: 520 PETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNE--FDFIS 577
Query: 344 GTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
GT++R + GE PP+ M P+ ++V+ +
Sbjct: 578 GTRMRKLAREGENPPDGFMAPKAWKVLTDY 607
>gb|AAF12761.1|AF173365_1 (AF173365) ATP sulfurylase/APS kinase isoform SK2 [Homo sapiens]
Length = 619
Score = 120 bits (298), Expect = 3e-26
Identities = 115/395 (29%), Positives = 178/395 (44%), Gaps = 46/395 (11%)
Query: 23 ILSEQHEY--PKVQIDHGRA------------IDLENI---AHGVYSPLKGFLTRED--- 62
I+ + HE P+ ++DH RA +DL+ + + G +PLKGF+ ++
Sbjct: 220 IIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQ 279
Query: 63 ---FESVLDHMRLSDDT-PWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIY 115
F+++LD M L D +IPIVL V +K E +L H +A + + Y
Sbjct: 280 VMHFDTLLDGMALPDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEFY 339
Query: 116 TYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETR 173
+ KEE +V+ T HP + V G +LVGG++++L ++ + +Y L P+E +
Sbjct: 340 EHRKEERCSRVWGTTCTKHPHIKMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELK 399
Query: 174 VLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYK 226
KE V AFQ RN H GH +Q ++ + +PVL G K D
Sbjct: 400 QKCKEMNADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVP 459
Query: 227 DEVIIKAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDH 285
+ +K + VL E ++A M YAGP E H R GA +IVGRD
Sbjct: 460 LDWRMKQHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDP 519
Query: 286 AGV------GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKY 338
AG+ + Y P + P L + + R A Y K M H+E
Sbjct: 520 AGMPHPETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNE-- 577
Query: 339 HVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
ISGT++R + GE PP+ M P+ ++V+ +
Sbjct: 578 FDFISGTRMRKLAREGENPPDGFMAPKAWKVLTDY 612
>pir||S44079 sulfate adenylyltransferase (EC 2.7.7.4) met3-1 - potato
>gi|2129999|pir||S67497 sulfate adenylyltransferase (EC
2.7.7.4) (clone StMet3-1) - potato
>gi|531495|emb|CAA52953.1| (X75041) sulfate
adenylyltransferase [Solanum tuberosum]
Length = 424
Score = 120 bits (297), Expect = 4e-26
Identities = 113/401 (28%), Positives = 178/401 (44%), Gaps = 38/401 (9%)
Query: 1 MVSKPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTR 60
++ +P GGKL+ R+ E PK+++ + ++ G SPLKGF+
Sbjct: 10 LLIEPDGGKLVELFVDESQRDLKRKEALNLPKIKLTKIDVEWVHVLSEGWASPLKGFMRE 69
Query: 61 EDFESVL--DHMRLSDDTP--WTIPIVL---DVEKPEFEEGDAI-LLYHKETPIARMHVE 112
+F L + +RL D + ++PIVL D +K + + ++ L+ PIA +
Sbjct: 70 SEFLQTLHFNSIRLEDGSVVNMSVPIVLAIDDSQKNQIDGSSSVALVVDGNNPIAILTDI 129
Query: 113 DIYTYEKEEFALKVFKTKDANHPGVAKVYS-MGKYLVGGEIELLNELP--NPFAKYTLRP 169
+IY + KEE + + T P V + + G +L+GG+++++ + + + L P
Sbjct: 130 EIYKHNKEERIARTWGTTAPGLPYVDEAITHSGNWLIGGDLKVIEPVKYHDGLDSFRLSP 189
Query: 170 IETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVLGRKKRGDYKDE 228
E R F + V AFQ RN H GH + ++ + NPVL G Y E
Sbjct: 190 SELRAEFTRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLHPLGGYTKE 249
Query: 229 VIIKAY-------EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIV 281
+ + +VL + + VL+ M YAGP E HA R N GA +IV
Sbjct: 250 DDVPLHWRMKQHEKVLEDGVLDPETTVLSIFPSPMHYAGPTEVQWHAKARINAGANFYIV 309
Query: 282 GRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGMVNE 329
GRD AG+ + P E DL+D L I P + A Y K GM
Sbjct: 310 GRDPAGMSH---PLEKRDLYDADHGKKVLSMAPGLERLNILP--FKVAAYDKTKNGMA-- 362
Query: 330 KICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + E PP+ M P ++V+
Sbjct: 363 FFDPSRPQDFIFISGTKMRTLAKTKESPPDGFMCPGGWKVL 403
>pir||JC4383 3'-phosphoadenosine-5'-phosphosulfate synthetase - spoonworm
(Urechis caupo) >gi|705385|gb|AAB00139.1| (L39001) PAPS
synthetase [Urechis caupo]
Length = 610
Score = 120 bits (297), Expect = 4e-26
Identities = 113/387 (29%), Positives = 171/387 (43%), Gaps = 31/387 (8%)
Query: 10 LIRRIAAPRTR-ERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLD 68
+++ + P + E +E + P ++I ++ ++ G +PL GF+ ++
Sbjct: 218 IVKELFVPESGLEHAKAEIVDLPTMEITKLDTQWVQVLSEGWATPLTGFMREREYLQSQH 277
Query: 69 HMRLSDD--TPWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKEEFA 123
L D T +IPIVL V +K E A L ++ IA + + Y + KEE
Sbjct: 278 FGCLLDGGVTNQSIPIVLPVHTADKDRLEGSSAFALSYEGKRIAILRTPEFYEHRKEERC 337
Query: 124 LKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNELP--NPFAKYTLRPIETRVLFKEKGW 181
+ F T +A P V + G +LVGG++E+L + + +Y L P E R F+
Sbjct: 338 SRQFGTSNAGQPYVKMIMESGDWLVGGDLEVLERITWNDGLDEYRLTPNELRAKFRALNA 397
Query: 182 KTVVAFQTRNVPHLGHEYVQ-KAALTFVDGLFINPVLGRKKRGDY-KDEVIIKAYE---- 235
V AFQ RN H GH + + + PVL G + KD+ + A+
Sbjct: 398 DAVFAFQLRNPVHNGHALLMTDTRRRLTERGYKKPVLLLHPLGGWTKDDDVPLAWRMKQH 457
Query: 236 --VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYG 293
+L E D V+A M YAGP E HA R + GA +IVGRD AG+ +
Sbjct: 458 QAILDEKVLDPDYTVMAIFPSPMMYAGPTEVQWHAKARMSTGANFYIVGRDPAGMPH--- 514
Query: 294 PYEAWDLFDEFPDL-------GITPMFI---REAFYCKKCGGMVNEKICPHDEKYHVRIS 343
P DL++ G+T + I R A Y K M HDE + IS
Sbjct: 515 PETKQDLYNATHGAKVLTMAPGLTQLEIVPFRVAAYNKTKSAMDFYDPERHDE--FMFIS 572
Query: 344 GTKLRNMIMRGEKPPEYMMRPEVYEVI 370
GTK+R M GE PP M P ++++
Sbjct: 573 GTKMRGMARAGETPPNGFMAPSAWKIM 599
>gb|AAC98687.1| (AF085144) ATP sulfurylase/APS kinase 2; PAPS synthetase [Mus
musculus]
Length = 616
Score = 120 bits (297), Expect = 4e-26
Identities = 110/388 (28%), Positives = 172/388 (43%), Gaps = 25/388 (6%)
Query: 9 KLIRRIAAPRTR-ERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVL 67
K I + P + ++I +E P + I ++ ++ G +PLKGF+ +++ L
Sbjct: 223 KGIHELFVPENKVDQIRAEAETLPSLPITKLDLQWVQILSEGWATPLKGFMREKEYLQTL 282
Query: 68 DHMRLSDD--TPWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKEEF 122
L DD +IPIVL V +K E L ++ +A + + Y + KEE
Sbjct: 283 HFDTLLDDGVINMSIPIVLPVSADDKARLEGCSKFALMYEGRRVALLQDPEFYEHRKEER 342
Query: 123 ALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKEKG 180
+V+ T A HP + V G +LVGG++++L + + +Y L P+E + K+
Sbjct: 343 CSRVWGTATAKHPHIKMVMESGDWLVGGDLQVLERIRWDDGLDQYRLTPLELKQKCKDMN 402
Query: 181 WKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVIIKA 233
V AFQ RN H GH +Q ++ + +PVL G K D E +K
Sbjct: 403 ADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLEWRMKQ 462
Query: 234 Y-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV---- 288
+ VL E ++A M YAGP E H R GA +IVGRD AG+
Sbjct: 463 HAAVLEERVLDPKSTIVAIFPSPMLYAGPTEVQWHCRCRMIAGANFYIVGRDPAGMPHPE 522
Query: 289 --GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGT 345
+ Y P + P L + + R A Y K M H+E ISGT
Sbjct: 523 TKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKIKKAMDFYDPARHEE--FDFISGT 580
Query: 346 KLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
++R + GE PP+ M P+ ++V+ +
Sbjct: 581 RMRKLAREGEDPPDGFMAPKAWKVLTDY 608
>gb|AAF40307.2| (AF160509) 3'-phosphoadenosine 5'-phosphosulfate synthetase 2 [Homo
sapiens]
Length = 614
Score = 120 bits (297), Expect = 4e-26
Identities = 113/390 (28%), Positives = 175/390 (43%), Gaps = 41/390 (10%)
Query: 23 ILSEQHEY--PKVQIDHGRA------------IDLENI---AHGVYSPLKGFLTREDFES 65
I+ + HE P+ ++DH RA +DL+ + + G +PLKGF+ +++
Sbjct: 220 IIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQ 279
Query: 66 VLDHMRLSDD--TPWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKE 120
V+ L DD +IPIVL V +K E +L H +A + + Y + KE
Sbjct: 280 VMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEFYEHRKE 339
Query: 121 EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKE 178
E +V+ T HP + V G +LVGG++++L ++ + +Y L P+E + KE
Sbjct: 340 ERCSRVWGTTCTKHPHIKMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKE 399
Query: 179 KGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVII 231
V AFQ RN H GH +Q ++ + +PVL G K D + +
Sbjct: 400 MNADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRM 459
Query: 232 KAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV-- 288
K + VL E ++A M YAGP E H R GA +IVGRD AG+
Sbjct: 460 KQHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPH 519
Query: 289 ----GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRIS 343
+ Y P + P L + + R A Y K M H+E IS
Sbjct: 520 PETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNE--FDFIS 577
Query: 344 GTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
GT++R + GE PP+ M P+ ++V+ +
Sbjct: 578 GTRMRKLAREGENPPDGFMAPKAWKVLTDY 607
>gi|11431665 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 2 [Homo
sapiens]
Length = 641
Score = 120 bits (297), Expect = 4e-26
Identities = 113/390 (28%), Positives = 175/390 (43%), Gaps = 41/390 (10%)
Query: 23 ILSEQHEY--PKVQIDHGRA------------IDLENI---AHGVYSPLKGFLTREDFES 65
I+ + HE P+ ++DH RA +DL+ + + G +PLKGF+ +++
Sbjct: 247 IIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQ 306
Query: 66 VLDHMRLSDD--TPWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTYEKE 120
V+ L DD +IPIVL V +K E +L H +A + + Y + KE
Sbjct: 307 VMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEFYEHRKE 366
Query: 121 EFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKE 178
E +V+ T HP + V G +LVGG++++L ++ + +Y L P+E + KE
Sbjct: 367 ERCSRVWGTTCTKHPHIKMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKE 426
Query: 179 KGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDEVII 231
V AFQ RN H GH +Q ++ + +PVL G K D + +
Sbjct: 427 MNADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRM 486
Query: 232 KAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV-- 288
K + VL E ++A M YAGP E H R GA +IVGRD AG+
Sbjct: 487 KQHAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPH 546
Query: 289 ----GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKYHVRIS 343
+ Y P + P L + + R A Y K M H+E IS
Sbjct: 547 PETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNE--FDFIS 604
Query: 344 GTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
GT++R + GE PP+ M P+ ++V+ +
Sbjct: 605 GTRMRKLAREGENPPDGFMAPKAWKVLTDY 634
>emb|CAA11416.1| (AJ223498) ATP sulfurylase [Brassica juncea]
Length = 470
Score = 118 bits (294), Expect = 8e-26
Identities = 119/410 (29%), Positives = 187/410 (45%), Gaps = 61/410 (14%)
Query: 4 KPHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLENI---AHGVYSPLKGFLTR 60
+P GGKL+ + ++ R++ + E V+I R +DLE + + G SPLKGF+ +
Sbjct: 56 EPDGGKLMD-LVVEESKRRVMKREAETVPVRIMLSR-VDLEWVHVLSEGWASPLKGFMRQ 113
Query: 61 EDFESVL--DHMRLSDDTP--WTIPIVL---DVEKPEFEEGDAILLYHKE-TPIARMHVE 112
+F L + +RL D + ++PIVL D +K + D + L P+A +
Sbjct: 114 SEFLQTLHFNSIRLEDGSVVNMSVPIVLAIDDDQKSRIGDSDRVTLVDSSGNPVAILSDI 173
Query: 113 DIYTYEKEEFALKVFKTKDANHPGVA----KVYSMGKYLVGGEIELLNELP--NPFAKYT 166
+IY + KEE ++ +T PG+ + G +L+GG++++L + + ++
Sbjct: 174 EIYKHPKEE---RIARTWGTTAPGLPYAEEAITRSGNWLIGGDLQVLEPIKYNDGLDRFR 230
Query: 167 LRPIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQKAALTFVDG-------------LFI 213
L P + R F ++ V AFQ RN H GH AL D L +
Sbjct: 231 LSPSQLREEFTKRDADAVFAFQLRNPVHNGH------ALLMTDTRRRLLEMGYKNPVLLL 284
Query: 214 NPVLGRKKRGDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRK 272
NP+ G K D ++ +E VL + + V++ M YAGP E HA R
Sbjct: 285 NPLGGFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHAKARI 344
Query: 273 NFGATHFIVGRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYC 320
N GA +IVGRD AG+G+ P E DL+D L I P + A Y
Sbjct: 345 NAGANFYIVGRDPAGMGH---PTEKRDLYDADHGKQVLSMAPGLERLNILP--FKVAAYD 399
Query: 321 KKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
G M P + + ISGTK+R + + E PP+ M P ++V+
Sbjct: 400 TTQGKMA--FFDPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVL 447
>gb|AAF70194.1| (AF251798) adenosine 5'-phosphosulfate kinase/ATP sulfurylase 2
[Cavia porcellus]
Length = 620
Score = 118 bits (293), Expect = 1e-25
Identities = 112/395 (28%), Positives = 175/395 (43%), Gaps = 34/395 (8%)
Query: 9 KLIRRIAAPRTR-ERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTRED----- 62
K I + P + +++ +E P + I ++ ++ G +PLKGF+ ++
Sbjct: 222 KGIHELFVPENKLDQVRTEAESLPSLSITKLDLQWVQILSEGWATPLKGFMREKEYLQTL 281
Query: 63 -FESVLDHMRLSDDT-PWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTY 117
F+++LD M L D ++PIVL V +K E L + +A + + Y +
Sbjct: 282 HFDTLLDGMVLRDGVINLSVPIVLPVSADDKARLEGCSEFALMYGGRRVAILCDPEFYEH 341
Query: 118 EKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVL 175
K E +V+ T A HP V V G++LVGG++++L + + KY L P+E +
Sbjct: 342 RKVERCCRVWGTSSAKHPHVKMVMESGEWLVGGDLQVLERIRWNDGLDKYRLTPLELKQK 401
Query: 176 FKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDE 228
KE V AFQ RN H GH +Q ++ + +PVL G K D +
Sbjct: 402 CKEMNADAVFAFQLRNPVHNGHALLMQDTHRQLLERGYKHPVLLLHPLGGWTKDDDVPLD 461
Query: 229 VIIKAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAG 287
+K + VL E ++A M YAGP E H R GA +IVGRD AG
Sbjct: 462 WRMKQHTAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRCRMIAGANFYIVGRDPAG 521
Query: 288 V------GNYYGPYEAWDLFDEFPDL---GITPMFIREAFYCKKCGGMVNEKICPHDEKY 338
+ + Y P + P L I P + KK N + HDE
Sbjct: 522 MPHPETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKVKKAMDFYNPE--RHDE-- 577
Query: 339 HVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
ISGT++R + GE PP+ M P+ ++V+ +
Sbjct: 578 FDFISGTRMRKLAREGENPPDGFMAPKAWKVLTDY 612
>pir||T40044 probable sulfate adenylyltransferase - fission yeast
(Schizosaccharomyces pombe) (fragment)
>gi|6018685|emb|CAB57930.1| (AL121795) putative sulfate
adenylyltransferase [Schizosaccharomyces pombe]
Length = 232
Score = 116 bits (288), Expect = 4e-25
Identities = 74/214 (34%), Positives = 115/214 (53%), Gaps = 9/214 (4%)
Query: 20 RERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDDTPWT 79
RE++ E PK+ + + D+E I +G +SPL GF+ ++D+ +V++++RLS +
Sbjct: 17 REQLEQEATSLPKIVLSERQFCDVELILNGGFSPLDGFMNQKDYLNVVENLRLSTGEVFP 76
Query: 80 IPIVLDVEKPE---FEEGDAILLYHK---ETPIARMHVEDIYTYEKEEFALKVFKTKDAN 133
IPI LD+ + + + GD + L +T IA + VED YT +K A KVF D
Sbjct: 77 IPITLDLNESQADSLKAGDRVALLDPRDGQTVIAILTVEDKYTPDKANEAEKVFGANDRA 136
Query: 134 HPGVAKVYS-MGKYLVGGEIELLNELPN-PFAKYTLRPIETRVLFKEKGWKTVVAFQTRN 191
HP V ++ G VGG+++ + + + F +Y P + R F+ W VVAFQTRN
Sbjct: 137 HPAVDYLFGRAGNVYVGGKLQAVTPIRHFDFVEYRYSPAQLRSDFQRNNWNRVVAFQTRN 196
Query: 192 VPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGD 224
H H E +AA + I+PV+G K GD
Sbjct: 197 PMHRAHRELTVRAAKQHGARVLIHPVVGMTKPGD 230
>gb|AAF18998.1|AF212154_1 (AF212154) ATP-sulfurylase [Allium cepa]
Length = 461
Score = 116 bits (288), Expect = 4e-25
Identities = 123/404 (30%), Positives = 186/404 (45%), Gaps = 45/404 (11%)
Query: 4 KPHGGKLIRRI--AAPRTRERILSEQHEYPKVQIDHGRAIDLENI---AHGVYSPLKGFL 58
+P GG L + A P R+ ++ + +DLE + + G SPL GF+
Sbjct: 44 EPDGGSLKNLVVPAGPARDTRVKEAATAGQALRRVRLKRVDLEWVHVLSEGWASPLGGFM 103
Query: 59 TREDFESVL--DHMRLSDDT--PWTIPIVL---DVEKPEFEEGDAILLYHK-ETPIARMH 110
+F L + +RL D + ++PIVL D +K E E +LL + + +A ++
Sbjct: 104 RESEFLQTLHFNSIRLDDGSFVNMSVPIVLAIDDEKKNEIGERKRVLLVDQNDKAVAFLN 163
Query: 111 VEDIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTL 167
+IY + KEE + + T P V + + + G +LVGG++E++ + + +Y L
Sbjct: 164 DIEIYKHNKEERIARTWGTTARGLPYVEEAIINAGNWLVGGDLEVIEPIKYNDGLDQYRL 223
Query: 168 RPIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVLGRKKRGDYK 226
P + R F + V AFQ RN H GH + ++ + NP+L G Y
Sbjct: 224 SPSQLRDEFSRRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGYT 283
Query: 227 --DEVII----KAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHF 279
D+V + K +E VL E + V+A M YAGP E HA R N GA +
Sbjct: 284 KADDVPLSWRMKQHEKVLEEGVLNPETTVVAIFPSPMHYAGPTEVQWHAKARINAGANFY 343
Query: 280 IVGRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGGM- 326
IVGRD AG+G+ P E DL+D L I P R A Y K G M
Sbjct: 344 IVGRDPAGMGH---PIEKRDLYDADHGKKVLSMAPGLEKLNILP--FRVAAYDKTQGKMD 398
Query: 327 VNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
++ P D V ISGTK+R++ E PP+ M P ++V+
Sbjct: 399 FFDQSRPED---FVFISGTKMRSLAKNKENPPDGFMCPGGWKVL 439
>sp|O88428|PPS2_MOUSE BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHETHASE 2
(PAPS SYNTHETHASE 2) (PAPSS 2) (SULFURYLASE KINASE 2)
(SK2) (SK 2) [INCLUDES: SULFATE ADENYLYLTRANSFERASE
(SULFATE ADENYLATE TRANSFERASE) (SAT) (ATP-SULFURYLASE);
ADENYLYLSULFATE KINASE (> >gi|3342266|gb|AAC40191.1|
(AF052453) ATP sulfurylase/APS kinase 2 [Mus musculus]
Length = 621
Score = 116 bits (287), Expect = 5e-25
Identities = 110/393 (27%), Positives = 175/393 (43%), Gaps = 30/393 (7%)
Query: 9 KLIRRIAAPRTR-ERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTRED----- 62
K I + P + ++I +E P + I ++ ++ G +PLKGF+ ++
Sbjct: 223 KGIHELFVPENKVDQIRAEAETLPSLPITKLDLQWVQILSEGWATPLKGFMREKEYLQTL 282
Query: 63 -FESVLDHMRLSDDT-PWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTY 117
F+++LD + D +IPIVL V +K E L ++ +A + + Y +
Sbjct: 283 HFDTLLDGVVPRDGVINMSIPIVLPVSADDKARLEGCSKFALMYEGRRVALLQDPEFYEH 342
Query: 118 EKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVL 175
KEE +V+ T A HP + V G +LVGG++++L + + +Y L P+E +
Sbjct: 343 RKEERCSRVWGTATAKHPHIKMVMESGDWLVGGDLQVLERIRWDDGLDQYRLTPLELKQK 402
Query: 176 FKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDE 228
K+ V AFQ RN H GH +Q ++ + +PVL G K D E
Sbjct: 403 CKDMNADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLE 462
Query: 229 VIIKAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAG 287
+K + VL E ++A M YAGP E H R GA +IVGRD AG
Sbjct: 463 WRMKQHAAVLEERVLDPKSTIVAIFPSPMLYAGPTEVQWHCRCRMIAGANFYIVGRDPAG 522
Query: 288 V------GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKYHV 340
+ + Y P + P L + + R A Y K M H+E
Sbjct: 523 MPHPETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKIKKAMDFYDPARHEE--FD 580
Query: 341 RISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
ISGT++R + GE PP+ M P+ ++V+ +
Sbjct: 581 FISGTRMRKLAREGEDPPDGFMAPKAWKVLTDY 613
>gi|6754984 3'-phosphoadenosine 5'-phosphosulfate synthase 2 [Mus musculus]
>gi|6466024|gb|AAF12760.1| (AF172866) ATP
sulfurylase/APS kinase isoform SK2 [Mus musculus]
Length = 621
Score = 115 bits (284), Expect = 1e-24
Identities = 110/393 (27%), Positives = 174/393 (43%), Gaps = 30/393 (7%)
Query: 9 KLIRRIAAPRTR-ERILSEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTRED----- 62
K I + P + ++I +E P I ++ ++ G +PLKGF+ ++
Sbjct: 223 KGIHELFVPENKVDQIRAEAETLPSPPITKLDLQWVQILSEGWATPLKGFMREKEYLQTL 282
Query: 63 -FESVLDHMRLSDDT-PWTIPIVLDV---EKPEFEEGDAILLYHKETPIARMHVEDIYTY 117
F+++LD + D +IPIVL V +K E L ++ +A + + Y +
Sbjct: 283 HFDTLLDGVVPRDGVINMSIPIVLPVSADDKARLEGCSKFALMYEGRRVALLQDPEFYEH 342
Query: 118 EKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVL 175
KEE +V+ T A HP + V G +LVGG++++L + + +Y L P+E +
Sbjct: 343 RKEERCSRVWGTATAKHPHIKMVMESGDWLVGGDLQVLERIRWDDGLDQYRLTPLELKQK 402
Query: 176 FKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL------GRKKRGDYKDE 228
K+ V AFQ RN H GH +Q ++ + +PVL G K D E
Sbjct: 403 CKDMNADAVYAFQLRNPVHNGHALMMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLE 462
Query: 229 VIIKAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAG 287
+K + VL E ++A M YAGP E H R GA +IVGRD AG
Sbjct: 463 WRMKQHTAVLEERVLDPKSTIVAIFPSPMLYAGPTEVQWHCRCRMIAGANFYIVGRDPAG 522
Query: 288 V------GNYYGPYEAWDLFDEFPDL-GITPMFIREAFYCKKCGGMVNEKICPHDEKYHV 340
+ + Y P + P L + + R A Y K M H+E
Sbjct: 523 MPHPETKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKIKKAMDFYDPARHEE--FD 580
Query: 341 RISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
ISGT++R + GE PP+ M P+ ++V+ +
Sbjct: 581 FISGTRMRKLAREGEDPPDGFMAPKAWKVLTDY 613
>pir||S44943 sulfate adenylyltransferase (EC 2.7.7.4) met3-1 precursor -
Arabidopsis thaliana >gi|2129743|pir||S68024 sulfate
adenylyltransferase (EC 2.7.7.4) precursor (clone APS2)
- Arabidopsis thaliana >gi|487404|emb|CAA55799.1|
(X79210) sulfate adenylyltransferase [Arabidopsis
thaliana] >gi|1228104|gb|AAA92351.1| (U06276) ATP
sulfurylase [Arabidopsis thaliana]
>gi|1378028|gb|AAC49324.1| (U40715) ATP sulfurylase
precursor [Arabidopsis thaliana]
>gi|1575324|gb|AAB09471.1| (U59737) ATP sulfurylase
[Arabidopsis thaliana]
>gi|10086481|gb|AAG12541.1|AC007797_1 (AC007797) sulfate
adenylyltransferase [Arabidopsis thaliana]
Length = 476
Score = 114 bits (282), Expect = 2e-24
Identities = 118/404 (29%), Positives = 184/404 (45%), Gaps = 52/404 (12%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHE-YPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTR 60
P GG+L+ I P T + + E PKV+++ IDLE I+ G SPLKGF+
Sbjct: 67 PDGGELVELIV-PETEIGVKKAESETMPKVKLNQ---IDLEWVHVISEGWASPLKGFMRE 122
Query: 61 EDFESVL--DHMRLSDDT--PWTIPIVLDVEKPEFEE----GDAILLYHKETPIARMHVE 112
+++ L + +RL + T ++PIVL ++ E+ + L+ + I +
Sbjct: 123 DEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLRSV 182
Query: 113 DIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRP 169
+IY + KEE + + T P V + + G +L+GG++E+ + + Y L P
Sbjct: 183 EIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSP 242
Query: 170 IETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------GRKKR 222
+ R F + V AFQ RN H GH + ++ + NPVL G K
Sbjct: 243 KQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKA 302
Query: 223 GDYKDEVIIKAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIV 281
D +V ++ + +VL + +++ M YAGP E HA R N GA +IV
Sbjct: 303 DDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIV 362
Query: 282 GRDHAGVGNYYGPYEAWDLFDEFPDLG------------ITPMFIREAFYCKKCGGMVNE 329
GRD AG+G+ P E DL+D PD G + + R A Y + +
Sbjct: 363 GRDPAGMGH---PTEKRDLYD--PDHGKRVLSMAPGLEKLNILPFRVAAY-----DTIEK 412
Query: 330 KIC---PHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
K+ P K + ISGTK+R GE PP+ M P + V+
Sbjct: 413 KMAFFDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVL 456
>pir||T14475 sulfate adenylyltransferase (EC 2.7.7.4) ASBo precursor - wild
cabbage >gi|1589913|gb|AAB67995.1| (U69694)
ATP-sulfurylase precursor [Brassica oleracea]
Length = 483
Score = 113 bits (281), Expect = 3e-24
Identities = 115/403 (28%), Positives = 181/403 (44%), Gaps = 50/403 (12%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTRE 61
P GG+L+ + E E PKV++ IDLE I+ G SPLKGF+ +
Sbjct: 71 PDGGELVELVVPDSEIELKKKEAESMPKVKLTK---IDLEWVHVISEGWASPLKGFMRED 127
Query: 62 DFESVL--DHMRLSDDT--PWTIPIVLDVEKPEFEE----GDAILLYHKETPIARMHVED 113
++ L + +RL D + ++PIVL ++ E+ + L+ + + + +
Sbjct: 128 EYLQSLHFNSLRLKDGSLVNMSLPIVLAIDDQTKEQIGVSKNVALVSPQGDIVGSLRSVE 187
Query: 114 IYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRPI 170
IY + KEE + + T P V + + G +L+GG++E+ + + Y L P
Sbjct: 188 IYKHNKEERIARTWGTTSPALPYVEEHITPSGNWLIGGDLEVFQPVKYNDGLDHYRLSPK 247
Query: 171 ETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------GRKKRG 223
+ R F + V AFQ RN H GH + ++ + NP+L G K
Sbjct: 248 QLRKEFDNRKADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPILLLHPLGGFTKAD 307
Query: 224 DYKDEVIIKAY-EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVG 282
D +V ++ + +VL + + +++ M YAGP E HA R N GA +IVG
Sbjct: 308 DVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVG 367
Query: 283 RDHAGVGNYYGPYEAWDLFDEFPDLG------------ITPMFIREAFYCKKCGGMVNEK 330
RD AG+G+ P E DL+D PD G + + R A Y V +K
Sbjct: 368 RDPAGMGH---PTEKRDLYD--PDHGKKVLSMAPGLEKLNILPFRVAAY-----DTVEKK 417
Query: 331 IC---PHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
+ P K + ISGTK+R GE PP+ M P + V+
Sbjct: 418 MAFFDPTRAKEFLFISGTKMRTYRRTGESPPDGFMCPSGWNVL 460
>gb|AAF13064.1|AF195511_1 (AF195511) ATP sulfurylase precursor [Brassica oleracea var.
botrytis]
Length = 483
Score = 113 bits (281), Expect = 3e-24
Identities = 114/403 (28%), Positives = 177/403 (43%), Gaps = 50/403 (12%)
Query: 5 PHGGKLIRRIAAPRTRERILSEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTRE 61
P GG+L+ + E E PKV++ IDLE I+ G SPLKGF+ +
Sbjct: 71 PDGGELMELVVPDSEIELKKKEAESMPKVKLTK---IDLEWVHVISEGWASPLKGFMRED 127
Query: 62 DFESVL--DHMRLSDDT--PWTIPIVLDVEKPEFEE----GDAILLYHKETPIARMHVED 113
++ L + +RL D + ++PIVL ++ E+ + L+ + + + +
Sbjct: 128 EYLQSLHFNSLRLKDGSLVNMSLPIVLAIDDQTKEQIGVSKNVALVSPQGDIVGSLRSVE 187
Query: 114 IYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRPI 170
IY + KEE + + T P V + + G +L+GG++E+ + + Y L P
Sbjct: 188 IYKHNKEERIARTWGTTSPGLPYVEEHITPSGNWLIGGDLEVFKPVKYNDGLDHYRLSPK 247
Query: 171 ETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVLGRKKRGDYKDEV 229
+ R F + V AFQ RN H GH + ++ + NPVL G +
Sbjct: 248 QLRKEFDNRKADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKAD 307
Query: 230 IIKAY-------EVLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVG 282
+ Y +VL + + +++ M YAGP E HA R N GA +IVG
Sbjct: 308 DVPLYVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVG 367
Query: 283 RDHAGVGNYYGPYEAWDLFDEFPDLG------------ITPMFIREAFYCKKCGGMVNEK 330
RD AG+G+ P E DL+D PD G + + R A Y V +K
Sbjct: 368 RDPAGMGH---PTEKRDLYD--PDHGKKVLSMAPGLEKLNILPFRVAAY-----DTVEKK 417
Query: 331 IC---PHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
+ P K + ISGTK+R GE PP+ M P + V+
Sbjct: 418 MAFFDPTRAKEFLFISGTKMRTYARTGESPPDGFMCPSGWNVL 460
>gi|6754982 3'-phosphoadenosine 5'-phosphosulfate synthase 1 [Mus musculus]
>gi|6647719|sp|Q60967|PPS1_MOUSE BIFUNCTIONAL
3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHETHASE 1
(PAPS SYNTHETHASE 1) (PAPSS 1) (SULFURYLASE KINASE 1)
(SK1) (SK 1) [INCLUDES: SULFATE ADENYLYLTRANSFERASE
(SULFATE ADENYLATE TRANSFERASE) (SAT) (ATP-SULFURYLASE);
ADENYLYLSULFATE KINASE (> >gi|1109676|gb|AAC52328.1|
(U34883) ATP sulfurylase/APS kinase [Mus musculus]
>gi|1586680|prf||2204316A ATP sulfurylase-adenosine
phosphosulfate kinase [Mus musculus]
Length = 624
Score = 111 bits (276), Expect = 1e-23
Identities = 101/352 (28%), Positives = 158/352 (44%), Gaps = 34/352 (9%)
Query: 46 IAHGVYSPLKGFLTREDFESVLDHMRLSDD--TPWTIPIVLDVEKPEFEEGD---AILLY 100
+A G +PL GF+ ++ L L D ++PIVL + E D A L
Sbjct: 270 LAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALV 329
Query: 101 HKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL-- 158
++ +A + + + + KEE + + T NHP + V G +L+GG++++L+ +
Sbjct: 330 YEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVLEQGDWLIGGDLQVLDRIYW 389
Query: 159 PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL 217
+ +Y L P E + FK+ V AFQ RN H GH +Q ++ + PVL
Sbjct: 390 NDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVL 449
Query: 218 GRKKRGDY-KDEVIIKAYE------VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIM 270
G + KD+ + + VL E + V+A M YAGP E H
Sbjct: 450 LLHPLGGWTKDDDVPLMWRMKQHAAVLEEGILDPETTVVAIFPSPMMYAGPTEVQWHCRA 509
Query: 271 RKNFGATHFIVGRDHAGV------GNYYGPYEAWDLFDEFP---DLGITPMFIREAFYCK 321
R GA +IVGRD AG+ + Y P + P L I P R A Y K
Sbjct: 510 RMVAGANFYIVGRDPAGMPHPETGKDLYEPTHGAKVLTMAPGLITLEIVP--FRVAAYNK 567
Query: 322 KCGGMVNEKICPHDEKYHVR---ISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
K +++ +D ++H ISGT++R + G+KPPE M P+ + V+
Sbjct: 568 K-----KKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVL 614
>pir||JW0087 3'-phosphoadenosine-5'-phosphosulfate synthetase - human
>gi|3378101|gb|AAC28429.1| (AF033026) bifunctional ATP
sulfurylase/adenosine 5'-phosphosulfate kinase [Homo
sapiens] >gi|7211186|gb|AAF40235.1| (AF097721)
3'-phosphoadenosine 5'-phosphosulfate synthetase [Homo
sapiens]
Length = 624
Score = 111 bits (276), Expect = 1e-23
Identities = 101/355 (28%), Positives = 159/355 (44%), Gaps = 34/355 (9%)
Query: 46 IAHGVYSPLKGFLTREDFESVLDHMRLSDD--TPWTIPIVLDVEKPEFEEGD---AILLY 100
+A G +PL GF+ ++ L L D ++PIVL + E D A L
Sbjct: 270 LAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALM 329
Query: 101 HKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL-- 158
++ +A + + + + KEE + + T NHP + V G +L+GG++++L+ +
Sbjct: 330 YEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYW 389
Query: 159 PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL 217
+ +Y L P E + FK+ V AFQ RN H GH +Q ++ + PVL
Sbjct: 390 NDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVL 449
Query: 218 GRKKRGDY-KDEVIIKAYE------VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIM 270
G + KD+ + + VL E + V+A M YAGP E H
Sbjct: 450 LLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRA 509
Query: 271 RKNFGATHFIVGRDHAGV------GNYYGPYEAWDLFDEFP---DLGITPMFIREAFYCK 321
R GA +IVGRD AG+ + Y P + P L I P R A Y K
Sbjct: 510 RMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVP--FRVAAYNK 567
Query: 322 KCGGMVNEKICPHDEKYHVR---ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
K +++ +D ++H ISGT++R + G+KPPE M P+ + V+ +
Sbjct: 568 K-----KKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEY 617
>sp|O43252|PPS1_HUMAN BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHETHASE 1
(PAPS SYNTHETHASE 1) (PAPSS 1) (SULFURYLASE KINASE 1)
(SK1) (SK 1) [INCLUDES: SULFATE ADENYLYLTRANSFERASE
(SULFATE ADENYLATE TRANSFERASE) (SAT) (ATP-SULFURYLASE);
ADENYLYLSULFATE KINASE (> >gi|2673862|emb|CAA71413.1|
(Y10387) PAPS sunthetase [Homo sapiens]
Length = 624
Score = 111 bits (275), Expect = 1e-23
Identities = 101/355 (28%), Positives = 159/355 (44%), Gaps = 34/355 (9%)
Query: 46 IAHGVYSPLKGFLTREDFESVLDHMRLSDD--TPWTIPIVLDVEKPEFEEGD---AILLY 100
+A G +PL GF+ ++ L L D ++PIVL + E D A L
Sbjct: 270 LAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALM 329
Query: 101 HKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL-- 158
++ +A + + + + KEE + + T NHP + V G +L+GG++++L+ +
Sbjct: 330 YEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYW 389
Query: 159 PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL 217
+ +Y L P E + FK+ V AFQ RN H GH +Q ++ + PVL
Sbjct: 390 NDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVL 449
Query: 218 GRKKRGDY-KDEVIIKAYE------VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIM 270
G + KD+ + + VL E + V+A M YAGP E H
Sbjct: 450 LLHPLGAWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRA 509
Query: 271 RKNFGATHFIVGRDHAGV------GNYYGPYEAWDLFDEFP---DLGITPMFIREAFYCK 321
R GA +IVGRD AG+ + Y P + P L I P R A Y K
Sbjct: 510 RMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVP--FRVAAYNK 567
Query: 322 KCGGMVNEKICPHDEKYHVR---ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
K +++ +D ++H ISGT++R + G+KPPE M P+ + V+ +
Sbjct: 568 K-----KKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEY 617
>emb|CAB89007.1| (AL353866) sulfate adenylyltransferase; methionine biosynthesis
[Schizosaccharomyces pombe]
Length = 295
Score = 111 bits (274), Expect = 2e-23
Identities = 72/194 (37%), Positives = 106/194 (54%), Gaps = 20/194 (10%)
Query: 191 NVPHLGH-EYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDVAVL 249
N H H E +AA + I+PV+G K GD ++ YE + + Y PK A L
Sbjct: 1 NPMHRAHRELTVRAAKQHGARVLIHPVVGMTKPGDIDHFTRVRVYEAILQRY-PKGSAKL 59
Query: 250 ATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVG------NYYGPYEAWDLFDE 303
+ + MR AGPREA+ HAI+RKN+GA+HFI+GRDHAG G ++YGPY+A L ++
Sbjct: 60 SLLPLAMRMAGPREALWHAIIRKNYGASHFIIGRDHAGPGKNSQGEDFYGPYDAQYLVEQ 119
Query: 304 F-PDLGITPMFIREAFYCKKCGGMVNEKICPHDEKYH-----VRISGTKLRNMIMRGEKP 357
+ ++GIT + + Y + +E I +K + ISGT+LR + G
Sbjct: 120 YAQEIGITIVPFQMMTY------LPDEDIYKPVDKVEPGTRTLNISGTELRRRLRVGANI 173
Query: 358 PEYMMRPEVYEVIR 371
PE+ PEV ++R
Sbjct: 174 PEWFSYPEVVAILR 187
>gi|4885537 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1 [Homo
sapiens] >gi|2853267|gb|AAC39894.1| (U53447) PAPS
synthase [Homo sapiens]
Length = 623
Score = 110 bits (273), Expect = 2e-23
Identities = 100/353 (28%), Positives = 156/353 (43%), Gaps = 33/353 (9%)
Query: 47 AHGVYSPLKGFLTREDFESVLDHMRLSDD--TPWTIPIVLDVEKPEFEEGD---AILLYH 101
A G +PL GF+ ++ L L D ++PIVL + E D A L +
Sbjct: 271 AEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMY 330
Query: 102 KETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL--P 159
+ +A + + + + KEE + + T NHP + V G +L+GG++++L+ +
Sbjct: 331 EGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWN 390
Query: 160 NPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVLG 218
+ +Y L P E + FK+ V AFQ RN H GH +Q ++ + PVL
Sbjct: 391 DGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLL 450
Query: 219 RKKRGDYKDEVIIKAYE------VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRK 272
G KD+ + + VL E + V+A M YAGP E H R
Sbjct: 451 LHPLGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARM 510
Query: 273 NFGATHFIVGRDHAGV------GNYYGPYEAWDLFDEFP---DLGITPMFIREAFYCKKC 323
GA +IVGRD AG+ + Y P + P L I P R A Y KK
Sbjct: 511 VAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVP--FRVAAYNKK- 567
Query: 324 GGMVNEKICPHDEKYHVR---ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
+++ +D ++H I GT++R + G+KPPE M P+ + V+ +
Sbjct: 568 ----KKRMDYYDSEHHEDFEFILGTRMRKLAREGQKPPEGFMAPKAWTVLTEY 616
>pir||S68202 ATP sulfurylase (clone APS2) precursor - Arabidopsis thaliana
(fragment)
Length = 407
Score = 109 bits (270), Expect = 5e-23
Identities = 111/383 (28%), Positives = 174/383 (44%), Gaps = 50/383 (13%)
Query: 25 SEQHEYPKVQIDHGRAIDLE---NIAHGVYSPLKGFLTREDFESVL--DHMRLSDDT--P 77
+E PKV+++ IDLE I+ G SPLKGF+ +++ L + +RL + T
Sbjct: 18 AESETMPKVKLNQ---IDLEWVHVISEGWASPLKGFMREDEYLQSLHFNSLRLKNGTFVN 74
Query: 78 WTIPIVLDVEKPEFEE----GDAILLYHKETPIARMHVEDIYTYEKEEFALKVFKTKDAN 133
++PIVL ++ E+ + L+ + I + +IY + KEE + + T
Sbjct: 75 MSLPIVLAIDDDTKEQIGSSENVALVCPQGDIIGSLRSVEIYKHNKEERIARTWGTTSPG 134
Query: 134 HPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTLRPIETRVLFKEKGWKTVVAFQTR 190
P V + + G +L+GG++E+ + + Y L P + R F + V AFQ R
Sbjct: 135 LPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLDHYRLSPKQLREEFDNRQADAVFAFQLR 194
Query: 191 NVPHLGHEYVQK-AALTFVDGLFINPVL------GRKKRGDYKDEVIIKAY-EVLFEHYY 242
N H GH + ++ + NPVL G K D +V ++ + +VL +
Sbjct: 195 NPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVL 254
Query: 243 PKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDLFD 302
+++ M YAGP E HA R N GA +IVGRD AG+G+ P E DL+D
Sbjct: 255 DPKTTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGH---PTEKRDLYD 311
Query: 303 EFPDLG------------ITPMFIREAFYCKKCGGMVNEKIC---PHDEKYHVRISGTKL 347
PD G + + R A Y + +K+ P K + ISGTK+
Sbjct: 312 --PDHGKRVLSMAPGLEKLNILPFRVAAY-----DTIEKKMAFFDPSRAKEFLFISGTKM 364
Query: 348 RNMIMRGEKPPEYMMRPEVYEVI 370
R GE PP+ M P + V+
Sbjct: 365 RTYARTGENPPDGFMCPSGWNVL 387
>gb|AAF40236.1|AF105227_1 (AF105227) 3'-phosphoadenosine 5'-phosphosulfate synthetase [Homo
sapiens]
Length = 624
Score = 109 bits (270), Expect = 5e-23
Identities = 100/355 (28%), Positives = 158/355 (44%), Gaps = 34/355 (9%)
Query: 46 IAHGVYSPLKGFLTREDFESVLDHMRLSDD--TPWTIPIVLDVEKPEFEEGD---AILLY 100
+A G +PL GF+ ++ L L D ++PIVL + E D A L
Sbjct: 270 LAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALM 329
Query: 101 HKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL-- 158
++ +A + + + + KEE + + T NHP + V G +L+GG++++L+ +
Sbjct: 330 YEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYW 389
Query: 159 PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL 217
+ +Y L P E + FK+ V AFQ RN H GH +Q ++ + PVL
Sbjct: 390 NDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVL 449
Query: 218 GRKKRGDY-KDEVIIKAYE------VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIM 270
G + KD+ + + VL E + V+A M YAGP E H
Sbjct: 450 LLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRA 509
Query: 271 RKNFGATHFIVGRDHAGV------GNYYGPYEAWDLFDEFP---DLGITPMFIREAFYCK 321
R GA +IVGRD AG+ + Y P + P L I P R A Y K
Sbjct: 510 RMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVP--FRVAAYNK 567
Query: 322 KCGGMVNEKICPHDEKYHVR---ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
K +++ +D ++H I GT++R + G+KPPE M P+ + V+ +
Sbjct: 568 K-----KKRMDYYDSEHHEDFEFILGTRMRKLAREGQKPPEGFMAPKAWTVLTEY 617
>gb|AAD09325.1| (AF016496) ATP sulfurylase/APS kinase [Homo sapiens]
Length = 624
Score = 106 bits (262), Expect = 5e-22
Identities = 98/355 (27%), Positives = 157/355 (43%), Gaps = 34/355 (9%)
Query: 46 IAHGVYSPLKGFLTREDFESVLDHMRLSDD--TPWTIPIVLDVEKPEFEEGD---AILLY 100
+A G +PL GF+ ++ L L D ++PIVL + E D A L
Sbjct: 270 LAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALM 329
Query: 101 HKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAKVYSMGKYLVGGEIELLNEL-- 158
++ +A + + + + KEE + + T NHP + V G +L+GG++++L+ +
Sbjct: 330 YEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYW 389
Query: 159 PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHE-YVQKAALTFVDGLFINPVL 217
+ +Y L P E + FK+ V AFQ RN H GH +Q ++ + PVL
Sbjct: 390 NDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVL 449
Query: 218 GRKKRGDY-KDEVIIKAYE------VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIM 270
G + KD+ + + VL E + V+A M YAGP E H
Sbjct: 450 LLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRA 509
Query: 271 RKNFGATHFIVGRDHAGV------GNYYGPYEAWDLFDEFP---DLGITPMFIREAFYCK 321
R GA ++ GRD AG+ + Y P + P L I P R A Y K
Sbjct: 510 RMVAGANFYMCGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVP--FRVAAYNK 567
Query: 322 KCGGMVNEKICPHDEKYHVR---ISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSF 373
K +++ +D ++H I GT++R + G+KPPE M P+ + V+ +
Sbjct: 568 K-----KKRMDYYDSEHHEDFEFILGTRMRKLAREGQKPPEGFMAPKAWTVLTEY 617
>sp|O54820|PPS1_CAVPO BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHETHASE 1
(PAPS SYNTHETHASE 1) (PAPSS 1) (SULFURYLASE KINASE 1)
(SK1) (SK 1) [INCLUDES: SULFATE ADENYLYLTRANSFERASE
(SULFATE ADENYLATE TRANSFERASE) (SAT) (ATP-SULFURYLASE);
ADENYLYLSULFATE KINASE (> >gi|2735717|gb|AAC02266.1|
(AF004875) 3'-phosphoadenosine 5'-phosphosulfate
synthase [Cavia porcellus]
Length = 624
Score = 106 bits (261), Expect = 6e-22
Identities = 101/376 (26%), Positives = 167/376 (43%), Gaps = 34/376 (9%)
Query: 25 SEQHEYPKVQIDHGRAIDLENIAHGVYSPLKGFLTREDFESVLDHMRLSDD--TPWTIPI 82
++ P +QI+ ++ +A G +PL GF+ ++ L L D ++PI
Sbjct: 249 TDAESLPALQINKVDMQWVQVLAEGWATPLGGFMREREYLQCLHFDCLLDGGVINLSVPI 308
Query: 83 VLDVEKPEFEEGD---AILLYHKETPIARMHVEDIYTYEKEEFALKVFKTKDANHPGVAK 139
VL + + E D A L ++ +A + + + + KEE + + T +HP +
Sbjct: 309 VLTATEEDKERLDGCTAFALIYEGRRVAILRNPEFFEHRKEERCARQWGTTCKSHPYIKM 368
Query: 140 VYSMGKYLVGGEIELLNEL--PNPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGH 197
V G +L+GG++++L+ + + +Y L P E + FK+ V AFQ RN H GH
Sbjct: 369 VMEQGDWLIGGDLQVLDRIYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGH 428
Query: 198 E-YVQKAALTFVDGLFINPVLGRKKRGDY-KDEVIIKAYE------VLFEHYYPKDVAVL 249
+Q ++ + PVL G + KD+ + + VL E + V+
Sbjct: 429 ALLMQDTHRQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGILNPESTVV 488
Query: 250 ATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV------GNYYGPYEAWDLFDE 303
A M YAGP H R GA +IVGRD AG+ + Y P +
Sbjct: 489 AIFPSPMMYAGPTGVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYKPTHGAKVLTM 548
Query: 304 FP---DLGITPMFIREAFYCKKCGGMVNEKICPHDEKYHVR---ISGTKLRNMIMRGEKP 357
P L I P R A Y K+ +++ +D ++H ISGT++R + G+KP
Sbjct: 549 APGLITLEIVP--FRVAAYNKR-----KKRMDYYDAEHHEDFEFISGTRMRRLAREGQKP 601
Query: 358 PEYMMRPEVYEVIRSF 373
PE M P + V+ +
Sbjct: 602 PEGFMAPTAWAVLAEY 617
>pir||T01204 sulfate adenylyltransferase (EC 2.7.7.4) - maize
>gi|2738750|gb|AAB94542.1| (AF016305) ATP sulfurylase
[Zea mays]
Length = 489
Score = 104 bits (256), Expect = 2e-21
Identities = 116/413 (28%), Positives = 183/413 (44%), Gaps = 47/413 (11%)
Query: 4 KPHGGKLIRRIAAPR--TRERILSEQHEYPKVQIDHGRAIDLENI---AHGVYSPLKGFL 58
+P GG+L+ +A R + E E P ++ GR +D E + + G SPL+GF+
Sbjct: 72 EPDGGRLVDLVAPEEGGRRAALRREAAELPH-RLRLGR-VDKEWVHVLSEGWASPLQGFM 129
Query: 59 TREDFESVLDHMRLSDD----TPWTIPIVL---DVEKPEFEEGDAI---LLYHKETPIAR 108
++F L + ++PIVL D ++ + A ++ ++ PIA
Sbjct: 130 RDDEFLQTLHFNAIRGQDGRMVNMSVPIVLSVGDAQRRAIQADGATRVAVVDERDRPIAV 189
Query: 109 MHVEDIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKY 165
+ +IY + KEE + + T P V + + + G +L+GG++E++ + + +Y
Sbjct: 190 LSDIEIYKHNKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVIEPIKYNDGLDQY 249
Query: 166 TLRPIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------G 218
L P + R F + V AFQ RN H GH + ++ + NPVL G
Sbjct: 250 RLSPAQLREEFARRNADAVFAFQLRNPVHNGHALLMTDTRKRLLEMGYKNPVLLPHPLGG 309
Query: 219 RKKRGDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGAT 277
K D +K +E VL E + V+A M YAGP E HA R N GA
Sbjct: 310 FTKADDVPLSWRMKQHEKVLEEGVLNPESTVVAIFPSPMHYAGPTEVQWHAKARINAGAN 369
Query: 278 HFIVGRDHAGVGNYYGPYEAWDLFDE------------FPDLGITPMFIREAFYCKKCGG 325
+IVGRD AG+ + P E DL+D L I P + A Y K
Sbjct: 370 FYIVGRDPAGMSH---PTEKRDLYDADHGKKVLSMAPGLERLNILP--FKVAAYDTKQKK 424
Query: 326 MVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVIRSFDNPFV 378
M + P + + ISGTK+R + E PP+ M P ++V+ + + V
Sbjct: 425 M--DFFDPSRKDDFLFISGTKMRTLAKNRESPPDGFMCPGGWKVLVEYYDSLV 475
>dbj|BAA36274.1| (AB015204) plastidic ATP sulfurylase [Oryza sativa]
Length = 476
Score = 104 bits (256), Expect = 2e-21
Identities = 111/400 (27%), Positives = 177/400 (43%), Gaps = 38/400 (9%)
Query: 4 KPHGGKLIRRIAAPRTRERILSEQHEYP---KVQIDHGRAIDLENIAHGVYSPLKGFLTR 60
+P GG+L+ + R + + +V++ L ++ G SPL+GF+
Sbjct: 60 EPDGGRLVELVVPEEGGRREAARREAAALAHRVRLGRVETEWLHVLSEGWASPLRGFMRE 119
Query: 61 EDFESVL--DHMRLSDDTP--WTIPIVL---DVEKPEFEEGDA---ILLYHKETPIARMH 110
+F L + +R D ++PIVL D ++ E A L+ + P+A +
Sbjct: 120 AEFLQALHFNAIRGGDGAMVNMSVPIVLPLGDAQRRAIEASGARRVALVDAADRPLAVLS 179
Query: 111 VEDIYTYEKEEFALKVFKTKDANHPGVAK-VYSMGKYLVGGEIELLNELP--NPFAKYTL 167
+IY + KEE + + T P V + + + G +L+GG++E++ + + +Y L
Sbjct: 180 DIEIYKHNKEERIARTWGTTAPGLPYVDEAITNAGDWLIGGDLEVIEPIKYNDGLDQYRL 239
Query: 168 RPIETRVLFKEKGWKTVVAFQTRNVPHLGHEYVQK-AALTFVDGLFINPVL------GRK 220
P + R F + V AFQ RN H GH + ++ + NPVL G
Sbjct: 240 SPAQLREEFARRNADAVFAFQLRNPVHNGHALLMTDTRKRLLEMGYKNPVLLLHPLGGFT 299
Query: 221 KRGDYKDEVIIKAYE-VLFEHYYPKDVAVLATVRYEMRYAGPREAIHHAIMRKNFGATHF 279
K D +K +E VL E + V+A M YAGP E HA R N GA +
Sbjct: 300 KADDVPLSWRMKQHEKVLEEGVLNPESTVVAIFPSPMHYAGPTEVQWHAKARINAGANFY 359
Query: 280 IVGRDHAGVGNYYGPYEAWDLFDE---------FPDLGITPMFIREAFYCKKCGGMVNEK 330
IVGRD AG+G+ P E DL+D P L + +A Y K M +
Sbjct: 360 IVGRDPAGMGH---PTEKRDLYDADHGKKVLSMAPGLEKLNILPFKAAYDTKQKKM--DF 414
Query: 331 ICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEVI 370
P + + ISGTK+R + + PP+ M P ++V+
Sbjct: 415 FDPSRKDDFLFISGTKMRTLAKNRQSPPDGFMCPGGWKVL 454
>gb|AAF12780.1| (AF173364) ATP sulfurylase/APS kinase isoform SK2 [Homo sapiens]
Length = 265
Score = 76.1 bits (184), Expect = 6e-13
Identities = 73/258 (28%), Positives = 114/258 (43%), Gaps = 25/258 (9%)
Query: 140 VYSMGKYLVGGEIELLNELP--NPFAKYTLRPIETRVLFKEKGWKTVVAFQTRNVPHLGH 197
V G +LVGG++++L ++ + +Y L P+E + KE V AFQ RN H GH
Sbjct: 2 VMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKEMNADAVFAFQLRNPVHNGH 61
Query: 198 EYV-QKAALTFVDGLFINPVL------GRKKRGDYKDEVIIKAYEVLFEHYY--PKDVAV 248
+ Q ++ + +PVL G K D + +K + + E PK +
Sbjct: 62 ALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRMKQHAAVLEEGVLDPKST-I 120
Query: 249 LATVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGV------GNYYGPYEAWDLFD 302
+A M YAGP E H R GA +IVGRD AG+ + Y P +
Sbjct: 121 VAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPHPETKKDLYEPTHGGKVLS 180
Query: 303 EFPDL-GITPMFIREAFYCKKCGGM----VNEKICPHDEKYHV--RISGTKLRNMIMRGE 355
P L + + R A Y K M + P +++ ISGT++R + GE
Sbjct: 181 MAPGLTSVEIIPFRVAAYNKAKKAMDFYDPASSLLPPCNRHNEFDFISGTRMRKLAREGE 240
Query: 356 KPPEYMMRPEVYEVIRSF 373
PP+ M P+ ++V+ +
Sbjct: 241 NPPDGFMAPKAWKVLTDY 258
>pir||G81256 probable sulfate adenylyltransferase (EC 2.7.7.4) Cj1609 [imported]
- Campylobacter jejuni (strain NCTC 11168)
>gi|6969026|emb|CAB73597.1| (AL139079) possible sulfate
adenylyltransferase [Campylobacter jejuni]
Length = 386
Score = 70.2 bits (169), Expect = 4e-11
Identities = 54/241 (22%), Positives = 103/241 (42%), Gaps = 7/241 (2%)
Query: 131 DANHPGVAKVYSMGKYLVGGEIELLNELPNPFAKYTLRPIETRVLFKEKGWKTVVAFQTR 190
D P + MG + G+IE+ N K + I+ + K +
Sbjct: 104 DIFSPNTCSLEDMGTTCISGKIEIYNSQIEKI-KENFQQIKNNL----NAQKITAIVSSF 158
Query: 191 NVPHLGHEYVQKAALTFVDGLFINPVLGRKKRGDYKDEVIIKAYEVLFEHYYPKDVAVLA 250
+ H HE + + + D + I V G ++ E+ + ++Y P D +
Sbjct: 159 DPLHRAHERMFRWTIDKADLVVIFLVESFDVNG-FEFELKEAYLKKFIQNYLPPDRIFIF 217
Query: 251 TVRYEMRYAGPREAIHHAIMRKNFGATHFIVGRDHAGVGNYYGPYEAWDLFDEFP-DLGI 309
++ + +I+ K+ G T +VG++H G+G +Y + + D+F D GI
Sbjct: 218 PLKNINIFHAHLNPGLESIIAKSLGCTKLVVGQNHTGLGMFYDDNQPKTILDDFSKDYGI 277
Query: 310 TPMFIREAFYCKKCGGMVNEKICPHDEKYHVRISGTKLRNMIMRGEKPPEYMMRPEVYEV 369
+ + E +C +C +V+ + CPH +H+ + L++++ G PP +R EV V
Sbjct: 278 EVIVLPEFVFCDQCRMIVSTRSCPHGCHHHLHYNSQSLKDLLRAGIIPPAIFIRKEVSSV 337
Query: 370 I 370
I
Sbjct: 338 I 338
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.142 0.432
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 155939314
Number of Sequences: 2977
Number of extensions: 7024006
Number of successful extensions: 16478
Number of sequences better than 1.0e-10: 69
Number of HSP's better than 0.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 48
Number of HSP's that attempted gapping in prelim test: 16206
Number of HSP's gapped (non-prelim): 70
length of query: 379
length of database: 189,106,746
effective HSP length: 52
effective length of query: 327
effective length of database: 157,985,422
effective search space: 51661232994
effective search space used: 51661232994
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 166 (69.1 bits)