BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1605 (PAB1605) DE:PUTATIVE ASPARAGINE SYNTHETASE
(GLUTAMINE-HYDROLYZING) (EC 6.3.5.4)
(611 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||F75095 probable asparagine synthase (glutamine-hydrolyzing)... 1269 0.0
emb|CAA07658.1| (AJ007747) putative glutamine amidotransferase [... 351 2e-95
gb|AAB99058.1| (U67548) asparagine synthetase (asnB) [Methanococ... 292 9e-78
emb|CAA07652.1| (AJ007747) putative glutamine amidotransferase [... 269 6e-71
pir||S70877 hypothetical protein 2 - Vibrio cholerae >gi|1230578... 256 4e-67
sp|P54420|ASNB_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 256 4e-67
dbj|BAA33606.1| (AB012956) probable asparagine synthetase [Vibri... 255 1e-66
emb|CAA69108.1| (Y07786) ORF71x8 [Vibrio cholerae] 254 2e-66
gb|AAF34252.1|AF168003_7 (AF168003) putative asparagine syntheta... 250 3e-65
dbj|BAB05227.1| (AP001512) asparagine synthetase [Bacillus halod... 242 7e-63
sp|O05272|ASNO_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 240 3e-62
pir||F69837 asparagine synthase homolog yisO - Bacillus subtilis... 232 8e-60
pir||F83384 probable asparagine synthetase PA2084 [imported] - P... 228 2e-58
gb|AAF24002.1| (AF035937) WbpS [Pseudomonas aeruginosa] 226 8e-58
emb|CAB58327.1| (AL121855) putative amidotransferase [Streptomyc... 220 3e-56
pir||A83638 probable glutamine amidotransferase PA0051 [imported... 210 4e-53
pir||B83214 probable glutamine amidotransferase PA3459 [imported... 202 9e-51
dbj|BAB12569.1| (AB039379) asparagine synthase homolog [Streptom... 201 3e-50
dbj|BAA89484.1| (AB029550) LtsA [Corynebacterium glutamicum] 196 5e-49
gb|AAF17502.1|AF195615_10 (AF195615) PhzH [Pseudomonas chlororap... 192 7e-48
sp|Q10374|ASNH_MYCTU PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-H... 180 4e-44
gb|AAG02363.1|AF210249_22 (AF210249) asparagine synthetase [Stre... 176 8e-43
sp|P42113|ASNH_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 170 4e-41
pir||H82255 asparagine synthetase B, glutamine-hydrolyzing VC099... 143 4e-33
sp|P22106|ASNB_ECOLI ASPARAGINE SYNTHETASE B [GLUTAMINE-HYDROLYZ... 139 1e-31
pdb|1CT9|A Chain A, Crystal Structure Of Asparagine Synthetase B... 139 1e-31
dbj|BAA89376.1| (AB025342) ORF2 [Moritella marina] 137 3e-31
gb|AAB99117.1| (U67554) asparagine synthetase (asnB) [Methanococ... 136 1e-30
sp|Q58516|ASNH_METJA PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-H... 136 1e-30
emb|CAA96526.1| (Z72354) asparagine synthetase [Vicia faba] 135 2e-30
gb|AAF02775.1|AF190728_1 (AF190728) asparagine synthetase [Helia... 133 5e-30
dbj|BAA96252.1| (AB035248) asparagine synthetase [Astragalus sin... 133 5e-30
gb|AAF02776.1|AF190729_1 (AF190729) asparagine synthetase [Helia... 133 6e-30
dbj|BAA97313.1| (AB019236) asparagine synthetase [Arabidopsis th... 132 1e-29
gb|AAC72837.1| (AF095453) asparagine synthetase [Arabidopsis tha... 132 1e-29
gi|4502259 asparagine synthetase [Homo sapiens] >gi|114266|sp|P0... 131 2e-29
sp|O24661|ASNS_TRIVS ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 131 3e-29
gi|11421677 asparagine synthetase [Homo sapiens] >gi|3341715|gb|... 130 4e-29
gb|AAC72836.1| (AF095452) asparagine synthetase [Arabidopsis tha... 130 4e-29
pir||T08846 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 129 1e-28
pir||D82846 asparagine synthase B XF0118 [imported] - Xylella fa... 129 1e-28
gb|AAB48058.1| (L40327) asparagine synthetase [Medicago sativa] 129 1e-28
gb|AAB81011.1| (U89923) asparagine synthetase [Medicago sativa] 129 1e-28
pir||T50812 asparagine synthetase ASN3 - Arabidopsis thaliana >g... 128 2e-28
sp|P49094|ASNS_MAIZE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 128 2e-28
sp|P49078|ASNS_ARATH ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 128 2e-28
gb|AAF74755.1| (AF263432) asparagine synthetase [Helianthus annuus] 128 2e-28
dbj|BAA96251.1| (AB035247) asparagine synthetase [Astragalus sin... 128 2e-28
gb|AAC16325.1| (AF061740) asparagine synthetase [Elaeagnus umbel... 128 2e-28
sp|P49093|ASN2_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 128 2e-28
sp|Q43011|ASNS_ORYSA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 127 4e-28
sp|P31752|ASNS_ASPOF ASPARAGINE SYNTHASE [GLUTAMINE-HYDROLYZING]... 127 5e-28
emb|CAA67889.1| (X99552) asparagine synthetase [Asparagus offici... 127 5e-28
sp|P19251|ASN1_PEA ASPARAGINE SYNTHETASE, NODULE [GLUTAMINE-HYDR... 127 5e-28
sp|P49092|ASN1_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 126 6e-28
pir||JQ2245 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 125 1e-27
sp|Q61024|ASNS_MOUSE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 125 1e-27
sp|O24338|ASNS_SANAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 125 1e-27
prf||2207183A Asn synthetase [Rattus norvegicus] 125 2e-27
pir||JW0071 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 124 3e-27
pir||T39308 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 124 3e-27
sp|P19891|ASNS_CRIGR ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 123 5e-27
sp|P49088|ASNS_RAT ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]... 123 7e-27
gb|AAB95197.1| (U84118) asparagine synthetase [Aedes aegypti] 123 7e-27
emb|CAB57292.1| (AJ133522) asparagine synthetase (type-I) [Phase... 123 7e-27
pir||AJHUN2 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 122 1e-26
sp|P19252|ASN2_PEA ASPARAGINE SYNTHETASE, ROOT [GLUTAMINE-HYDROL... 122 2e-26
dbj|BAB17726.1| (AB050900) asparagine synthetase [Raphanus sativus] 121 2e-26
emb|CAA08913.1| (AJ009952) asparagine synthetase type II [Phaseo... 121 3e-26
sp|P49091|ASNS_BRAOL ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 121 3e-26
sp|P17714|ASNS_MESAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN... 120 6e-26
gi|6325403 asparagine synthetase; Asn1p [Saccharomyces cerevisia... 113 6e-24
gi|6321563 asparagine synthetase; Asn2p [Saccharomyces cerevisia... 113 6e-24
gb|AAC49614.1| (U77679) asparagine synthetase 1 [Glycine max] 113 6e-24
pir||T18441 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 113 8e-24
pir||T42010 probable asparagine synthase (glutamine-hydrolyzing)... 102 9e-21
pir||T16625 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 101 2e-20
pir||G75092 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 100 4e-20
pir||G71050 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.... 98 2e-19
emb|CAA62733.1| (X91388) unknown [Pseudomonas syringae] 85 3e-15
>pir||F75095 probable asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
PAB1605 - Pyrococcus abyssi (strain Orsay)
>gi|5458579|emb|CAB50067.1| (AJ248286) PUTATIVE
ASPARAGINE SYNTHETASE (GLUTAMINE-HYDROLYZING) (EC
6.3.5.4) [Pyrococcus abyssi]
Length = 611
Score = 1269 bits (3248), Expect = 0.0
Identities = 611/611 (100%), Positives = 611/611 (100%)
Query: 1 MCGINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKY 60
MCGINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKY
Sbjct: 1 MCGINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKY 60
Query: 61 EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMW 120
EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMW
Sbjct: 61 EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMW 120
Query: 121 AFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFL 180
AFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFL
Sbjct: 121 AFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFL 180
Query: 181 YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKD 240
YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKD
Sbjct: 181 YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKD 240
Query: 241 LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQ 300
LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQ
Sbjct: 241 LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQ 300
Query: 301 RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLL 360
RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLL
Sbjct: 301 RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLL 360
Query: 361 DGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVW 420
DGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVW
Sbjct: 361 DGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVW 420
Query: 421 VTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNA 480
VTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNA
Sbjct: 421 VTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNA 480
Query: 481 MRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFA 540
MRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFA
Sbjct: 481 MRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFA 540
Query: 541 TPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIM 600
TPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIM
Sbjct: 541 TPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIM 600
Query: 601 ELWLREWMDNG 611
ELWLREWMDNG
Sbjct: 601 ELWLREWMDNG 611
>emb|CAA07658.1| (AJ007747) putative glutamine amidotransferase [Bordetella
bronchiseptica]
Length = 628
Score = 351 bits (890), Expect = 2e-95
Identities = 230/643 (35%), Positives = 337/643 (51%), Gaps = 59/643 (9%)
Query: 1 MCGINGFSWG--DEELVKRMNDAIR---HRGPDDEG----VYVDDNVSLGHVRLAIIDLS 51
MCG+ G W DE R +R HRGPDD G +SLGH RLA+IDLS
Sbjct: 1 MCGVLGAWWRHHDEHEDARFAAGLRKLAHRGPDDRGSEHFAAPHGRLSLGHTRLAVIDLS 60
Query: 52 PKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD 111
GHQPM + D + +V+NGEIYN+ E+R EL + G +F ++DTEV+LAA+ WG
Sbjct: 61 AAGHQPMHADAD--RLAVVFNGEIYNYRELRSELRQIGASFRGDSDTEVLLAAWQHWGVA 118
Query: 112 CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKP 171
+ RF GM+AF +YD+ + L L+RD FGIKP+YY+ + ++F SE+ + Q +P P
Sbjct: 119 ALRRFKGMFAFALYDRRQQTLTLARDAFGIKPMYYHAAPERLLFGSELPAV-QALLPHPP 177
Query: 172 --NDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKR 229
N +D+L Y D DTF GI++L G+ DL T + +D E R
Sbjct: 178 RLNWQRAYDYLAYGQYDQPPDTFIAGIRQLPAGHLLTLDLRTGQAGEPMAWDDPASQECR 237
Query: 230 KEISDPQGFKDL-------FLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI 282
P GF++ FL V++ L +DVP+G+ LSGG+DSS+IVCAMR L PD I
Sbjct: 238 -----PIGFQEAAEELRARFLDNVRLHLRSDVPIGAALSGGIDSSAIVCAMRWLEPDLPI 292
Query: 283 KVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIY 342
+ FS + E ++ +V C + ++ ++L D+ DLIR Q EP + SIY
Sbjct: 293 QTFSFISDDTHRSEERWVDLVNQHCGATGHKIHIAPHELLDDLDDLIRVQGEPVGSTSIY 352
Query: 343 GQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRI 402
QYRV KLARE+G+ V LDGQG+DE+L GY + LL + G R + R
Sbjct: 353 AQYRVFKLAREHGVVVTLDGQGADELLGGYGGYPTERLASLLETGRLGAAARLL----RQ 408
Query: 403 HGSLVPLKNMVLYIL-------------------PVWVTKRLWRRRFPYLREEFIEKFKK 443
G P + + L P W+ ++ R + +RE
Sbjct: 409 QGRGAPARLLQAAALAAGGRHLANLRHATGRQHAPAWLDAQVLRDQGVTMREPAHAWAAA 468
Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
P + + L L A + L LLR D+N+MR+S+ESRVPF +L E+ LSL
Sbjct: 469 TPLRG-----RRLVNELAHALYHRGLGALLRHGDRNSMRFSVESRVPFLTQDLAEFALSL 523
Query: 504 PPESKVS-AGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDS 562
P VS +G TK + RK ++GI+PD I +R K+GFATP++ ++ Q ++D+
Sbjct: 524 PERYLVSDSGQTKHIFRKAMRGIVPDSILDRDDKIGFATPEQQWMQATAPQ-MRQWLVDA 582
Query: 563 ESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLR 605
P+ +V F+ ++G +S + W+ + + W++
Sbjct: 583 ADI---PFLRNAEVTAQFDAMIAGATPFSWQAWRWVNFQRWMQ 622
>gb|AAB99058.1| (U67548) asparagine synthetase (asnB) [Methanococcus jannaschii]
Length = 516
Score = 292 bits (739), Expect = 9e-78
Identities = 191/491 (38%), Positives = 273/491 (54%), Gaps = 51/491 (10%)
Query: 1 MCGINGFSWGDEELVK----RMNDAIRHRGPDDEGVYVDD----NVSLGHVRLAIIDLSP 52
MCGING +E++K +MN AI+HRGPDDEG+++ + ++ LGHVRLAI+DLS
Sbjct: 2 MCGINGIIRFGKEVIKEEINKMNKAIKHRGPDDEGIFIYNFKNYSIGLGHVRLAILDLSE 61
Query: 53 KGHQPMKY------------EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEV 100
KGHQPM Y E D ++ IVYNGEIYN++E++++ + T TDTEV
Sbjct: 62 KGHQPMGYNVDEDKIIYRDDELDRADIIIVYNGEIYNYLELKEKFNLETET---GTDTEV 118
Query: 101 ILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIK 160
IL Y + GFDCV+ FNGMWAF I+DK K ++F SRDR G+KP YYY+DG IFSSE+K
Sbjct: 119 ILKLYNKLGFDCVKEFNGMWAFCIFDKKKGLIFCSRDRLGVKPFYYYWDGNEFIFSSELK 178
Query: 161 GILQ-HNIPRKPN---DAVIFDFLYYNL-LDHTEDTFFEGIKRLMPGYNAVFDLN---TR 212
GIL I +K N DAV LY+ L + + ++ +L N +FDL+ R
Sbjct: 179 GILAVKEINKKENINKDAV---ELYFALGFIPSPYSIYKNTFKLEARQNLIFDLDKREIR 235
Query: 213 KFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA 272
K+ W+ D + +K+K I + K L AVK+R+ +DVPVG+ LSGGLDSS++V
Sbjct: 236 KYYYWELPDYKPIYDKKKLIEEG---KKLLYDAVKIRMRSDVPVGAFLSGGLDSSTVVGV 292
Query: 273 MRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQ 332
MR ++ FS+ F GK DE+ Y +IVVD + + F D I
Sbjct: 293 MREFTDLSKLHTFSIGFEGK-YDETPYIKIVVDYFKTQHHHYYFKERDFEELIDKYSWIY 351
Query: 333 EEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKL 392
+EPF S + Y+V ++AR+ + V+L G G DE+ GY Y ++ +RK
Sbjct: 352 DEPFGDYSGFPTYKVSEMARK-FVTVVLSGDGGDEVFGGYMTHLNGYRMDFIRK------ 404
Query: 393 LREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK---RPSKEL 449
+ + R+ GS +P+K + I +++ K +R E + E K+ RP
Sbjct: 405 ---LPKFLRVVGSKLPVKKDLNGIANLYLLKEAFRLSLINPEEFYAESIKEDAIRPEIYK 461
Query: 450 IWKIKTLNEAL 460
W I+ L L
Sbjct: 462 KWTIEKLRYCL 472
>emb|CAA07652.1| (AJ007747) putative glutamine amidotransferase [Bordetella
bronchiseptica]
Length = 636
Score = 269 bits (681), Expect = 6e-71
Identities = 204/651 (31%), Positives = 319/651 (48%), Gaps = 63/651 (9%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR-------HRGPDDEGVYVDDNVSLG--HVRLAIIDLS 51
MCGI G W + V + ++ HRGPD G ++D LG HVRL+IIDL
Sbjct: 1 MCGIFGALWHQGKSVSAIEQQLQVGIARQQHRGPDGHGTWIDPQGRLGLAHVRLSIIDLV 60
Query: 52 PKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD 111
G QPM+ I YNGEIYN++E+R+EL E + TS DTEVIL AY +WG
Sbjct: 61 -SGAQPMRGNSGNV---ITYNGEIYNYIELRRELGEDSFETTS--DTEVILRAYEKWGER 114
Query: 112 CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKP 171
CV+R GM+AF I+D + +F++RDRFGIKP YY F SE+K + + R
Sbjct: 115 CVDRLEGMFAFAIWDAKRGEMFVARDRFGIKPFYYACVQGGFYFGSEVKALTPF-LNRVE 173
Query: 172 NDA-VIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVF--DLNTRKFEAWK-YYDLRERIE 227
DA + D+ + E T F G+++L P + DL ++ + W+ YDL
Sbjct: 174 TDANALHDYFAFQFC-LGEKTLFAGVRQLKPAHCGYVGRDLELKQRQYWEVQYDLDWDHT 232
Query: 228 KRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSL 287
++ + + ++ +V M + +DV VG+ +SGG+DSS + RH + F+
Sbjct: 233 EKYFV---EQVREKLQHSVDMHMRSDVEVGAYISGGVDSSLVAAMARHGRRSSRFQGFTG 289
Query: 288 VFP-GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
F G DES+Y + + DE + ++ +D + DI +I ++P + + QY
Sbjct: 290 KFALGDAFDESRYAQALADEHDILLHQVDITEDDFVNDIAKVIYHLDQPVAGPGSFPQYA 349
Query: 347 VMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLL--REILAYKRIHG 404
V +LA+++ +KV+L GQGSDE+ GY + Y+ + ++ G + I+ Y+ I
Sbjct: 350 VSRLAKDH-VKVVLGGQGSDEMFGGYARYLIAYWEQCIKGALDGTMHDGNYIVTYESIIP 408
Query: 405 SLVPLKNMVLYILPVWVTKRLWRRRFPYLR-----------------EEFIEKFKKRPSK 447
+L L+N I R Y R E F K
Sbjct: 409 NLRTLRNYKPLIQEFMAEGIFDSRDKRYYRLINRSNTFGNVVNWGMFEGIASSFD--DFK 466
Query: 448 ELIWKIKTLNEALLLAETYYS----LPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
++ W E+ + T++ LP LL+ ED+ +M IESRVPF DH+L+E V ++
Sbjct: 467 QIFWGKNVGKESYFDSITHFDFKTLLPALLQVEDRMSMAHGIESRVPFLDHKLIELVATV 526
Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATP-DKDILKTNEGQKFALAVIDS 562
P K G K LLR + +LP IR R K+GF P + K + ++F L S
Sbjct: 527 PANVKFQNGELKRLLRVAFQDMLPQAIRERKDKMGFPVPLQVWMRKGGKAREFILDTFRS 586
Query: 563 ESFKKRPY----WDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLREWMD 609
++ + R Y +D K++ + +S+ +W + +ELW +++ D
Sbjct: 587 QNARSRAYLAPGFDIEKMMEQ-------EGLFSRNIWAFLSLELWQQQFHD 630
>pir||S70877 hypothetical protein 2 - Vibrio cholerae >gi|1230578|gb|AAC46243.1|
(U47057) orf2 [Vibrio cholerae O139]
>gi|1588972|prf||2209416B ORF 2 [Vibrio cholerae]
Length = 636
Score = 256 bits (648), Expect = 4e-67
Identities = 197/644 (30%), Positives = 316/644 (48%), Gaps = 48/644 (7%)
Query: 1 MCGINGF---SWGDEELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPKGH 55
MCG+ GF + + M ++I HRGP+D G++VDD+ V L H RL+I DLS GH
Sbjct: 1 MCGVAGFISKRLSPVDCLTSMVESIMHRGPNDSGLWVDDDFGVCLAHARLSIQDLSSAGH 60
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTF-TSNTDTEVILAAYLEWGFD-CV 113
QPM + K + V +++NGEIYN + +R+EL E ++ ++DTE +LA + WG + +
Sbjct: 61 QPM-HSKSERYV-MIFNGEIYNHLTLREELIEIVPSYWNGHSDTETLLAGFEVWGIEQTI 118
Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
++ GM+A V++DK L L RDRFG KPLYY + +F+SE+K + H + N
Sbjct: 119 QKCVGMFAIVLWDKVLKQLILIRDRFGEKPLYYGWQRDTFLFASELKALKAHPSFEGSIN 178
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
+ F N + T + +EGI +L PG AVF + ++D + +
Sbjct: 179 RQALSHFFRLNYIP-TPLSIYEGIFKLEPGVIAVFSHEGQLLSKQTFWDASHAV-SLQNF 236
Query: 233 SDPQG---FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVF 289
SD DL ++++ + ++DVP+G+ LSGG+DSS++V ++ L +K F++ F
Sbjct: 237 SDHDAVDKLDDLIKQSIQDQALSDVPLGAFLSGGVDSSTVVGILQS-LSTRPVKTFTIGF 295
Query: 290 PGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMK 349
+ +E+ V + ED LA I L +EPF+ S + V K
Sbjct: 296 DHADFNEASEASDVAKHLGTDHVELIVSAEDALAIINQLPVMYDEPFADASQVPTFLVSK 355
Query: 350 LARENGMKVLLDGQGSDEILAG---YHY---FFGY-------------YFVELLRKFKWG 390
LA++ + V L G G DE+ G YHY + Y F+ L W
Sbjct: 356 LAKKE-VTVCLSGDGGDELFCGYNRYHYTAKVWSYLEKIPFPIRKMLSVFLLTLSPSSWD 414
Query: 391 KLLREI--------LAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFK 442
L + + L K G+ LK + L V W P ++ +EK
Sbjct: 415 VLSKTLGLNTRLPNLGNKTQKGAQA-LKARDIEDLYTRVVSN-WDLDEPLVKNTAVEKLP 472
Query: 443 KRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLS 502
+ + L + +L + Y + +L D+ M S+E RVP DH + E+ S
Sbjct: 473 FLSDLTELSHLNDLEKMMLWDKQSYLMDDVLVKTDRATMACSLEGRVPLLDHRIAEFAAS 532
Query: 503 LPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDS 562
LP K G K LLR+ L +P ++ R K GF+ P + L+ + +A ++DS
Sbjct: 533 LPIHLKYRGGKGKWLLREVLYRYVPKKLIERPKK-GFSLPIAEWLR-GPLKDWANVLLDS 590
Query: 563 ESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLRE 606
+ F K + V + + EH++GK++WS +LW V++ +LWL +
Sbjct: 591 DRFDKEGFLSSELVQKKWGEHLAGKRDWSSQLWSVLMFQLWLEK 634
>sp|P54420|ASNB_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 1
>gi|7474447|pir||H69590 asparagine synthetase asnB -
Bacillus subtilis >gi|2293165|gb|AAC00243.1| (AF008220)
asparagine synthase [Bacillus subtilis]
>gi|2635538|emb|CAB15032.1| (Z99119) asparagine
synthetase [Bacillus subtilis]
Length = 632
Score = 256 bits (648), Expect = 4e-67
Identities = 183/620 (29%), Positives = 310/620 (49%), Gaps = 59/620 (9%)
Query: 12 EELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVY 71
EEL+K+MN I HRGPD +G + D++V G RL+IID+ G QP+ YE + WI++
Sbjct: 20 EELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVE-NGGQPLSYEDE--TYWIIF 76
Query: 72 NGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNI 131
NGEIYN++E+R+ELE KGYTF +++DTEV+LA Y + + + GM+AF+I++K+ ++
Sbjct: 77 NGEIYNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASKLRGMFAFLIWNKNDHV 136
Query: 132 LFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDT 191
L+ +RD FGIKPLYY + F+SE K ++ + + + ++ + + T
Sbjct: 137 LYGARDPFGIKPLYYTTINDQVYFASERKSLMVAQNDIEIDKEALQQYMSFQFVPE-PST 195
Query: 192 FFEGIKRLMPGYNAVFDLNTRKFEAWK-YYDLRERIEKRKEISDPQGFKDLFLRAVKMRL 250
+K++ PG + F + +K Y+ + + +E + +D +V + +
Sbjct: 196 LDAHVKKVEPG--SQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKEVRDAIYDSVNVHM 253
Query: 251 VADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVS 310
+DVPVGS LSGG+DSS IV + P +K FS+ F + E + +
Sbjct: 254 RSDVPVGSFLSGGIDSSFIVSVAKEFHP--SLKTFSVGFEQQGFSEVDVAKETAAALGIE 311
Query: 311 WYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILA 370
+ E+ + ++ ++ ++P + + Y V K A+++ + V L G+G+DE+
Sbjct: 312 NISKVISPEEYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKH-VTVALSGEGADELFG 370
Query: 371 GYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWVT----KRLW 426
GY+ + RE L+ K LK M+L++ V K L
Sbjct: 371 GYNIY------------------REPLSLKPFERIPSGLKKMLLHVAAVMPEGMRGKSLL 412
Query: 427 RRRFPYLREEFI---EKFKKRPSKELIWKIKTLNEALLLAETYYS--------------- 468
R L++ +I + F++ K+L+ + +TY++
Sbjct: 413 ERGCTPLQDRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVD 472
Query: 469 -----LPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLK 523
+L DK M S+E RVPF D + + +P E K G TK LLRK +
Sbjct: 473 IHTWMRGDILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAE 532
Query: 524 GILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEH 583
GI+P+ + NR K+GF P + LK NE ++ +I + DY VL++ E+H
Sbjct: 533 GIVPEHVLNR-KKLGFPVPIRHWLK-NEMNEWVRNIIQESQTDAYIHKDY--VLQLLEDH 588
Query: 584 VSGKKNWSQELWKVIIMELW 603
+ K + S+++W V+I +W
Sbjct: 589 CADKADNSRKIWTVLIFMIW 608
>dbj|BAA33606.1| (AB012956) probable asparagine synthetase [Vibrio cholerae]
Length = 636
Score = 255 bits (645), Expect = 1e-66
Identities = 195/643 (30%), Positives = 313/643 (48%), Gaps = 46/643 (7%)
Query: 1 MCGINGF---SWGDEELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPKGH 55
MCG+ GF + + M ++I HRGP+D G++VDD+ V L H RL+I DLS GH
Sbjct: 1 MCGVAGFISKRLSPVDCLTSMVESIMHRGPNDSGLWVDDDFGVCLAHARLSIQDLSSAGH 60
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTF-TSNTDTEVILAAYLEWGFD-CV 113
QPM + K + V +++NGEIYN + +R+EL E ++ ++DTE +LA + WG + +
Sbjct: 61 QPM-HSKSERYV-MIFNGEIYNHLTLREELIEIVPSYWNGHSDTETLLAGFEVWGIEQTI 118
Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
++ GM+A V++DK L L RDRFG KPLYY + +F+SE+K + H + N
Sbjct: 119 QKCVGMFAIVLWDKVLKQLILIRDRFGEKPLYYGWQRDTFLFASELKALKAHPSFEGSIN 178
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
+ F N + T + +EGI +L PG AVF + ++D + +
Sbjct: 179 RQALSHFFRLNYIP-TPLSIYEGIFKLEPGVIAVFSHEGQLLSKQTFWDASHAV-SLQNF 236
Query: 233 SDPQG---FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVF 289
SD DL ++++ + ++DVP+G+ LSGG+DSS++V ++ L +K F++ F
Sbjct: 237 SDHDAVDKLDDLIKQSIQDQALSDVPLGAFLSGGVDSSTVVGILQS-LSTRPVKTFTIGF 295
Query: 290 PGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMK 349
+ +E+ V + ED LA I L +EPF+ S + V K
Sbjct: 296 DHADFNEASEASDVAKHLGTDHVELIVSAEDALAIINQLPVMYDEPFADASQVPTFLVSK 355
Query: 350 LARENGMKVLLDGQGSDEILAG---YHY---FFGY-------------YFVELLRKFKWG 390
LA++ + V L G G DE+ G YHY + Y F+ L W
Sbjct: 356 LAKKE-VTVCLSGDGGDELFCGYNRYHYTAKVWSYLEKIPFPIRKMLSVFLLTLSPSSWD 414
Query: 391 KLLREILAYKR-------IHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK 443
L + + R I LK + L V W P ++ +EK
Sbjct: 415 VLSKTLGLNTRLPNLGNKIQKGAQALKARDIEDLYTRVVSN-WDLDEPLVKNTAVEKLPF 473
Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
+ + L + +L + Y + +L D+ M S+E RVP DH + E+ SL
Sbjct: 474 LSDLTELSHLNDLEKMMLWDKQSYLMDDVLVKTDRATMACSLEGRVPLLDHRIAEFAASL 533
Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSE 563
P K G K LLR+ L +P ++ R K GF+ P + L+ + +A ++DS+
Sbjct: 534 PIHLKYRGGKGKWLLREVLYRYVPKKLIERPKK-GFSLPIAEWLR-GPLKDWANVLLDSD 591
Query: 564 SFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLRE 606
K + V + + EH++GK++WS +LW V++ +LWL +
Sbjct: 592 RIDKEGFLSSELVQKKWREHLAGKRDWSSQLWSVLMFQLWLEK 634
>emb|CAA69108.1| (Y07786) ORF71x8 [Vibrio cholerae]
Length = 636
Score = 254 bits (642), Expect = 2e-66
Identities = 195/643 (30%), Positives = 312/643 (48%), Gaps = 46/643 (7%)
Query: 1 MCGINGF---SWGDEELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPKGH 55
MCG+ GF + + M +I HRGP+D G++VDD+ V L H RL+I DLS GH
Sbjct: 1 MCGVAGFISKRLSPVDCLTSMVGSIMHRGPNDSGLWVDDDFGVCLAHARLSIQDLSSAGH 60
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTF-TSNTDTEVILAAYLEWGFD-CV 113
QPM + K + V +++NGEIYN + +R+EL E ++ ++DTE +LA + WG + +
Sbjct: 61 QPM-HSKSERYV-MIFNGEIYNHLTLREELIEIVPSYWNGHSDTETLLAGFEVWGIEQTI 118
Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
++ GM+A V++DK L L RDRFG KPLYY + +F+SE+K + H + N
Sbjct: 119 QKCVGMFAIVLWDKVLKQLILIRDRFGEKPLYYGWQRDTFLFASELKALKAHPSFEGSIN 178
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
+ F N + T + +EGI +L PG AVF + ++D + +
Sbjct: 179 RQALSHFFRLNYIP-TPLSIYEGIFKLEPGVIAVFSHEGQLLSKQTFWDASHAV-SLQNF 236
Query: 233 SDPQG---FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVF 289
SD DL ++++ + ++DVP+G+ LSGG+DSS++V ++ L +K F++ F
Sbjct: 237 SDHDAVDKLDDLIKQSIQDQALSDVPLGAFLSGGVDSSTVVGILQS-LSTRPVKTFTIGF 295
Query: 290 PGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMK 349
+ +E+ V + ED LA I L +EPF+ S + V K
Sbjct: 296 DHADFNEASEASDVAKHLGTDHVELIVSAEDALAIINQLPVMYDEPFADASQVPTFLVSK 355
Query: 350 LARENGMKVLLDGQGSDEILAG---YHY---FFGY-------------YFVELLRKFKWG 390
LA++ + V L G G DE+ G YHY + Y F+ L W
Sbjct: 356 LAKKE-VTVCLSGDGGDELFCGYNRYHYTAKVWSYLEKIPFPIRKMLSVFLLTLSPSSWD 414
Query: 391 KLLREILAYKR-------IHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK 443
L + + R I LK + L V W P ++ +EK
Sbjct: 415 VLSKTLGLNTRLPNLGNKIQKGAQALKARDIEDLYTRVVSN-WDLDEPLVKNTAVEKLPF 473
Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
+ + L + +L + Y + +L D+ M S+E RVP DH + E+ SL
Sbjct: 474 LSDLTELSHLNDLEKMMLWDKQSYLMDDVLVKTDRATMACSLEGRVPLLDHRIAEFAASL 533
Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSE 563
P K G K LLR+ L +P ++ R K GF+ P + L+ + +A ++DS+
Sbjct: 534 PIHLKYRGGKGKWLLREVLYRYVPKKLIERPKK-GFSLPIAEWLR-GPLKDWANVLLDSD 591
Query: 564 SFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLRE 606
K + V + + EH++GK++WS +LW V++ +LWL +
Sbjct: 592 RIDKEGFLSSELVQKKWREHLAGKRDWSSQLWSVLMFQLWLEK 634
>gb|AAF34252.1|AF168003_7 (AF168003) putative asparagine synthetase [Desulfovibrio gigas]
Length = 643
Score = 250 bits (632), Expect = 3e-65
Identities = 195/642 (30%), Positives = 302/642 (46%), Gaps = 49/642 (7%)
Query: 1 MCGINGFSWGDE----------ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDL 50
MCGI GF + +++ M I HRGPD G +V + H RLAIIDL
Sbjct: 1 MCGIAGFVLLQDAPPVTDHQARDVLAAMAGRIAHRGPDGRGHHVGLPAAFAHCRLAIIDL 60
Query: 51 SPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGF 110
S G QPM E DG V + +NGEIYN+ E+R+EL+ G TF + +DTEV+L AYLEWG
Sbjct: 61 ST-GAQPMAAE-DGAAV-VTFNGEIYNYQEVRRELQALGRTFQTRSDTEVLLQAYLEWGV 117
Query: 111 DCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRK 170
C++R GM+AF ++D + LF +RDRFG KP +Y F+SE+ + +P
Sbjct: 118 ACLDRLEGMFAFALWDVRRRTLFAARDRFGKKPFFYTIQQGVFAFASELSAL--EAVPWA 175
Query: 171 P---NDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEA-WKYYDLRERI 226
+ + FL Y + E + E K Y + + T E W+ R
Sbjct: 176 AFSIRASTLARFLAYEYVPTPESIYAEARKLPPAQYLELHEDGTLLVERYWETPMPRPEG 235
Query: 227 EKRKEISDP----QGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI 282
K +D + + L +AV+ RL+ADVPVG LSGG+DSSS+ L P I
Sbjct: 236 SKGFGRADEPALCEELRRLLTQAVRRRLIADVPVGVFLSGGVDSSSVAAMAGQLAPG--I 293
Query: 283 KVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADII-DLIRTQEEPFSTLSI 341
K FS+ F DES Y V + ++ D AD++ +++ +EP + SI
Sbjct: 294 KTFSIGFTEASYDESAYAGRVAAQLGTD-HQLELLSADACADLLPEVVSRFDEPMADPSI 352
Query: 342 YGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKR 401
Y + ++ R + V L G G DE+LAGY Y+ G+ + + + A
Sbjct: 353 VPTYLLSQITRRQ-VTVALGGDGPDELLAGYEYYPGFKATQYWERLPAWLRRAAVAASGL 411
Query: 402 IHGSL-------VPLKNMVLYILPVWV-TKRLWRRRFPYLRE-------EFIEKFKKRPS 446
+ GS V + P W+ T+R+ P + + +E + S
Sbjct: 412 LPGSAGYVNPRHVAQTFLAAAEAPAWLRTQRMLTALTPEAQRALWKSPGDMLEPTRLFAS 471
Query: 447 KELIWKI----KTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLS 502
+ +W+ + L + ++L D+ M +E+R PF D +L E+
Sbjct: 472 TKRLWEALPDWPPMARVFHLFAQQITYGYILVKVDRCTMLHGLEARAPFLDRDLAEFCFR 531
Query: 503 LPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDS 562
LP K+ K LL++ + G+LP +I +R K GF P L+ + ++
Sbjct: 532 LPVSMKLRGATRKYLLKEAMAGLLPQDILHR-PKRGFLIPTAAWLR-GRLRPLVDDLLHE 589
Query: 563 ESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWL 604
+++ + V R+ EEH SGKK+ + LW +++++LWL
Sbjct: 590 RRLREQGLFHPAAVRRLVEEHASGKKDHRKGLWTMLVLQLWL 631
>dbj|BAB05227.1| (AP001512) asparagine synthetase [Bacillus halodurans]
Length = 615
Score = 242 bits (612), Expect = 7e-63
Identities = 171/560 (30%), Positives = 274/560 (48%), Gaps = 58/560 (10%)
Query: 1 MCGINGF-SW-----GDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI G+ W + E +K+M + HRGPDD V+ + + +LGH RL ++D P+G
Sbjct: 1 MCGITGWVDWRRNLQNETETIKQMAETQTHRGPDDLNVWTEKHAALGHSRLIVVD--PEG 58
Query: 55 H-QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
QPM E++GK IVYNGE+YN ++RKEL KGY F ++DTEV+L +Y+EWG+ CV
Sbjct: 59 GCQPMMRERNGKRYTIVYNGELYNTEDLRKELIVKGYQFQGHSDTEVLLVSYIEWGYQCV 118
Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
E+FNG++AF I++ LF++RDR G+KPL+Y ++F++EIK +L H I
Sbjct: 119 EKFNGIFAFAIWNDKDQSLFMARDRLGVKPLFYTVRHGFLLFATEIKALLAHPEIEPVLT 178
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
+ + + L + F+ I+ L P + +D N K +Y+ L+ +
Sbjct: 179 EEGLSEVLGLGPSRSPGNGVFDDIQELRPAHLLTYDRNGAKVS--RYWRLKSMAHSEDAM 236
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGK 292
+DL V+ +L ADVPVG LSGG+DSS++ A+ L+ + E K +
Sbjct: 237 ETAAHLRDLLEDTVERQLFADVPVGMFLSGGVDSSALT-AIAALIYEREGKGLIRTYSID 295
Query: 293 EIDESKYQRI--------------VVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFST 338
+ KY + V R + + ++E++ + + ++ P
Sbjct: 296 YDENDKYFKANDFQPNADGPWVEKVSTTFRTNHHNAVISIEELANQLKRAVELRDLPGMA 355
Query: 339 LSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLL--REI 396
Y K +++ + V L G+ +DEI GY +F + F W + R+
Sbjct: 356 DVDSSLYWFCKQIKKD-VTVGLSGECADEIFGGYPWFHKPEVMN-FNGFPWMRSADERQE 413
Query: 397 LAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRF---PYLREEFIEKFKKRPSKELIWKI 453
L ++R WR++ Y+ + + E + P E
Sbjct: 414 LLHER------------------------WRQKLNLPKYVHDRYKETIAETPRFEEDTPE 449
Query: 454 KTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGI 513
+ + + + LL +D+ +M S+E RVPF DH +VEY ++P E K G
Sbjct: 450 EARRREISYLNMVWFMTTLLDRKDRMSMGASLEVRVPFSDHRIVEYAWNIPWEIKQFGGR 509
Query: 514 TKVLLRKGLKGILPDEIRNR 533
K +LRK L+GILPDE+ R
Sbjct: 510 EKGILRKALEGILPDEVLYR 529
>sp|O05272|ASNO_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 3
>gi|1934835|emb|CAB07965.1| (Z93940) asparagine
synthetase [Bacillus subtilis]
Length = 614
Score = 240 bits (607), Expect = 3e-62
Identities = 169/558 (30%), Positives = 275/558 (48%), Gaps = 56/558 (10%)
Query: 1 MCGINGFSWGDEELVK------RMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI G+ ++LV+ RM D + RGPDD V+ + +V GH RLA++D+ G
Sbjct: 1 MCGITGWVDFKKQLVQEKQTMDRMTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEG-G 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QPM G I+YNGE+YN ++RKEL +G+ F +DTEV+L +Y+EW DCV+
Sbjct: 60 RQPMACTYKGDTYTIIYNGELYNTEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVD 119
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDA 174
NG++AF ++D+ +N+LF +RDR G+KP +Y +G + +F SEIK IL H + D
Sbjct: 120 HLNGIFAFAVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDR 179
Query: 175 VIFDFLYYNLLDHTEDT-FFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEIS 233
++ T T F+GIK + P + F + W+Y+++ EK +
Sbjct: 180 TGLSEIFGLGPSRTPGTGIFKGIKEIRPAHALTFSKD--GLNIWRYWNVES--EKHTDSF 235
Query: 234 DP--QGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVFP 290
D + LF AV +LV+DVPV + LSGGLDSS+I A H +G+ + +
Sbjct: 236 DDTVANVRSLFQDAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSID 295
Query: 291 GKEIDE----SKYQ--------RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFST 338
+E D+ S +Q + + + ++ + +D++ + + + ++ P
Sbjct: 296 YEENDKYFQASAFQPNDDGPWIEKMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLP-GM 354
Query: 339 LSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILA 398
+ + V L G+ +DEI GY +F + ++ F W + E +
Sbjct: 355 ADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWF---HTADVESGFPWMRSTEERI- 410
Query: 399 YKRIHGSLVPLKNMVLYILPVWVTKRLWRRRF---PYLREEFIEKFKKRPSKELIWKIKT 455
K + S W+++ Y+ ++ E + P + +
Sbjct: 411 -KLLSDS--------------------WQKKLNLKEYVNAKYEETLAETPLLDGETGVDK 449
Query: 456 LNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITK 515
L + + +LL +D+ +M S+E RVPF DH LVEYV ++P E K+ K
Sbjct: 450 ARRQLFYLNMLWFMTNLLDRKDRMSMGASLEVRVPFADHRLVEYVWNIPWEMKMHDNREK 509
Query: 516 VLLRKGLKGILPDEIRNR 533
+LRK L+GILPD+I R
Sbjct: 510 GILRKALEGILPDDILYR 527
>pir||F69837 asparagine synthase homolog yisO - Bacillus subtilis
>gi|2145380|emb|CAA70643.1| (Y09476) YisO [Bacillus
subtilis] >gi|2633415|emb|CAB12919.1| (Z99109) alternate
gene name: yucB~similar to asparagine synthase [Bacillus
subtilis]
Length = 591
Score = 232 bits (586), Expect = 8e-60
Identities = 161/535 (30%), Positives = 263/535 (49%), Gaps = 50/535 (9%)
Query: 18 MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYN 77
M D + RGPDD V+ + +V GH RLA++D+ G QPM G I+YNGE+YN
Sbjct: 1 MTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEG-GRQPMACTYKGDTYTIIYNGELYN 59
Query: 78 FMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRD 137
++RKEL +G+ F +DTEV+L +Y+EW DCV+ NG++AF ++D+ +N+LF +RD
Sbjct: 60 TEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVDHLNGIFAFAVWDEKRNLLFAARD 119
Query: 138 RFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDT-FFEGI 196
R G+KP +Y +G + +F SEIK IL H + D ++ T T F+GI
Sbjct: 120 RLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDRTGLSEIFGLGPSRTPGTGIFKGI 179
Query: 197 KRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDP--QGFKDLFLRAVKMRLVADV 254
K + P + F + W+Y+++ EK + D + LF AV +LV+DV
Sbjct: 180 KEIRPAHALTFSKD--GLNIWRYWNVES--EKHTDSFDDTVANVRSLFQDAVTRQLVSDV 235
Query: 255 PVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVFPGKEIDE----SKYQ--------R 301
PV + LSGGLDSS+I A H +G+ + + +E D+ S +Q
Sbjct: 236 PVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEENDKYFQASAFQPNDDGPWIE 295
Query: 302 IVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLD 361
+ + + ++ + +D++ + + + ++ P + + V L
Sbjct: 296 KMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLP-GMADVDSSLLWFCREIKKDFVVSLS 354
Query: 362 GQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWV 421
G+ +DEI GY +F + ++ F W + E + K + S
Sbjct: 355 GECADEIFGGYPWF---HTADVESGFPWMRSTEERI--KLLSDS---------------- 393
Query: 422 TKRLWRRRF---PYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDK 478
W+++ Y+ ++ E + P + + L + + +LL +D+
Sbjct: 394 ----WQKKLNLKEYVNAKYEETLAETPLLDGETGVDKARRQLFYLNMLWFMTNLLDRKDR 449
Query: 479 NAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNR 533
+M S+E RVPF DH LVEYV ++P E K+ K +LRK L+GILPD+I R
Sbjct: 450 MSMGASLEVRVPFADHRLVEYVWNIPWEMKMHDNREKGILRKALEGILPDDILYR 504
>pir||F83384 probable asparagine synthetase PA2084 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9948096|gb|AAG05472.1|AE004636_3 (AE004636) probable
asparagine synthetase [Pseudomonas aeruginosa]
Length = 610
Score = 228 bits (575), Expect = 2e-58
Identities = 187/567 (32%), Positives = 277/567 (47%), Gaps = 54/567 (9%)
Query: 1 MCGINGFS-WGDE-----ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI G++ W + +++RM + RGPD EG+++ + LGH RLAIIDL G
Sbjct: 1 MCGITGWTDWSRDLGEQRSILERMTRTLAPRGPDAEGLWLSRHALLGHRRLAIIDLE-NG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QPM E G+ V + Y+GE+YNF E+R ELE G F + +DTEV+L AYL+WG
Sbjct: 60 AQPMLAEAGGR-VALTYSGEVYNFRELRAELETLGQVFRTRSDTEVVLRAYLQWGEASFV 118
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDA 174
R G++ F ++D+ + L+L RDRFG+KPLYYY ++F+SE K I + DA
Sbjct: 119 RLRGIYGFALWDEREQSLWLVRDRFGVKPLYYYPTAGGVLFASEPKAIFANPEANPVLDA 178
Query: 175 V-IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLN-TRKFEAWKYYDLRERIEKRKEI 232
I + ++G++++ PG FD N R+ W R E+ +E
Sbjct: 179 SGIAELFALTTAPTPGHGLYKGLRQVRPGQALRFDRNGVRELVYWAL-----RAERHEEG 233
Query: 233 SDPQGFK--DLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE---IKVFSL 287
++ + L AV +LVADVP+GS LSGGLDSS+I A DG+ + FS+
Sbjct: 234 AEDSAERAGQLLREAVAEQLVADVPLGSLLSGGLDSSAI-SAFAVAALDGDARRLPTFSV 292
Query: 288 VFPG----------KEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFS 337
FPG + + Y ++ + E+VL +++ ++ P
Sbjct: 293 DFPGEGRGFVPTPWQSSWDEPYAQLAANFLGTPHRTVLVAPEEVLEQDEAVLQARDLPGW 352
Query: 338 TLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREIL 397
Y + + RE+ V L G+ +DE+ GY +F + F W L
Sbjct: 353 GELDASLYLLFRQVREH-TTVALSGESADEVFGGYPFFHDPSAL-AHDGFPW-------L 403
Query: 398 AYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLN 457
A K L+ + P ++ R +R + E +R +E+ +
Sbjct: 404 AGKSGPWQLLRREVAERVAAPEYIRAR-YREALAEVPRLDGEDAAQRRQREVAY------ 456
Query: 458 EALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVL 517
L L+ LP +L +D+ +M +E RVPFCDH LVEYV +LP K AG +K L
Sbjct: 457 --LALSRW---LPAMLERKDRMSMAVGLEVRVPFCDHRLVEYVWNLPWALKSVAGESKSL 511
Query: 518 LRKGLKGILPDEIRNRASKVGF-ATPD 543
LR+ L+G LP I R K GF A PD
Sbjct: 512 LRRALRGHLPQAILER-RKSGFPANPD 537
>gb|AAF24002.1| (AF035937) WbpS [Pseudomonas aeruginosa]
Length = 627
Score = 226 bits (569), Expect = 8e-58
Identities = 186/643 (28%), Positives = 308/643 (46%), Gaps = 57/643 (8%)
Query: 1 MCGINGFSW-------GDEELVKR-MNDAIRHRGPDDEGVYVDDNVS--LGHVRLAIIDL 50
MCGI GF W GD V R M AI HRGPD+ G++ + + L H RLA+++L
Sbjct: 1 MCGIAGF-WNITGTLLGDNARVARQMAAAIHHRGPDESGIWYEAPRAPILVHARLAVLEL 59
Query: 51 SPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFT--SNTDTEVILAAYLEW 108
SP G QPM D ++YNGEIYN + +R L E G T + +DTE +LA + +W
Sbjct: 60 SPAGSQPM--HSDCGRYVLIYNGEIYNHLALRARLSEAGVTHSWRGGSDTETLLACFAQW 117
Query: 109 GFDCVERFN-GMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNI 167
G + + GM+A ++D+ + + L+RDR G KPLY+ + + F+SE+K + +H +
Sbjct: 118 GVESTLKLTVGMFALALWDRQEKTITLARDRMGEKPLYWGWQNGVLFFASELKALKEHPL 177
Query: 168 PRKPNDA-VIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNT--RKFEAWKYYDLRE 224
R D + FL Y + + ++GI +L G V + E Y+
Sbjct: 178 FRGDIDRDALALFLRYGYVP-APYSIYKGIGKLRAGSYLVLSERSLNETCEPAAYWSANA 236
Query: 225 RIEKRKEISDP-QG--------FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRH 275
IE + +S+P QG + ++ ++V+DVP+G+ LSGG+DSS++V M+
Sbjct: 237 AIE--EALSNPFQGTDAEAVDLLESQLRTSISDQMVSDVPLGAFLSGGVDSSTVVALMQQ 294
Query: 276 LLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEP 335
I+ FS+ F DE+ Y + V + N +D L I L + EP
Sbjct: 295 -QSSRPIRTFSIGFDEPGYDEAVYAKAVAEHIGTDHTELYVNSKDALDVIPSLPKIYCEP 353
Query: 336 FSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYH-YFFGYYFVELLRKFKWGKLLR 394
F S V LAR+ + V L G G DE+ GY+ Y F +L +F
Sbjct: 354 FGDSSQIPTLIVSGLARQQ-VTVALSGDGGDELFGGYNPYQFTPRVWRMLERFP-----H 407
Query: 395 EILAYKRIHGSLVPLKNMVLYILPVWVTKRL----------WR-RRFPYLREEFIEKFKK 443
+ + +PL + + V+ ++ WR +P + +
Sbjct: 408 SMRRFASAFAQDLPLPEKLGKLRDVFASRTAEELFYRLNSHWRNHEYPVIGAQGHTALLD 467
Query: 444 RPSKELIW-KIKTLNEALLLAETYYSLP-HLLRFEDKNAMRWSIESRVPFCDHELVEYVL 501
P + W ++ + ++ + +P +L D+ AM S+E+RVP DH + E
Sbjct: 468 TPER---WPRVDSFQHWMMAMDVQGYMPDDILVKVDRAAMANSLETRVPLIDHRVFELAW 524
Query: 502 SLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVID 561
+P K+ G K LLR+ L + E+ R K GF+ P D L+ +++A +++D
Sbjct: 525 RMPLHMKIRNGKGKWLLREVLYRHVSRELIERPKK-GFSVPVSDWLR-GPLKEWAESLLD 582
Query: 562 SESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWL 604
++ Y D R + R++ +H++G+++ S+ LW V++ + WL
Sbjct: 583 ERRLQQEGYLDSRLIRRIWNDHLAGRRDHSRRLWSVLMFQAWL 625
>emb|CAB58327.1| (AL121855) putative amidotransferase [Streptomyces coelicolor
A3(2)]
Length = 644
Score = 220 bits (556), Expect = 3e-56
Identities = 184/662 (27%), Positives = 305/662 (45%), Gaps = 89/662 (13%)
Query: 1 MCGING-FSWGDEELVK-RMNDAIRHRGPDDEGVYV----DDNVSLGHVRLAIIDLSPKG 54
MCGI G + W D + V R+ D + HRGPD G Y + V LGH RLA++DLS G
Sbjct: 1 MCGIAGTYHWPDGKAVTDRLTDVLAHRGPDGAGRYSHPAGEGEVHLGHRRLAVVDLSTTG 60
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QPM E + + YNGE+YN E+R ELE G F +DTEV+L A+ WG DC+
Sbjct: 61 AQPMVSEG----LALSYNGELYNAPELRTELESAGVRFRGTSDTEVVLEAWRRWGTDCLP 116
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND- 173
+ GM+AF ++D+ L L+RD+ GIKPL+ G ++F+SE+K + + D
Sbjct: 117 QLRGMFAFAVFDERTGELVLARDQLGIKPLFLLRRGAGLVFASELKALATATGGKLEVDH 176
Query: 174 -AVIFDFLYYNLLDHTEDTFFEGIKRLMPG--YNAVFDLNTRKFEAWKYYDLRERIEKRK 230
A++ LYY + D ++L PG D + W D+ +R
Sbjct: 177 AALVASLLYYWVPD--SRCALREAEKLPPGSWLRCRPDGRVERGRFWNLRDVAAEGRERA 234
Query: 231 EISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFP 290
+ + + + L++DVPV + LSGGLDSS + P I +++ F
Sbjct: 235 RSGERPDLAAVVEESTRRHLLSDVPVATFLSGGLDSSYLTALAARDRPG--ISAYTIGFR 292
Query: 291 GKEI------DESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQ----EEPFSTLS 340
++ D+ +Y R V + V + ++ +++DL+ +EP +
Sbjct: 293 AEDARFEAMPDDLRYARQVAERFGVDLHEI-----EIAPNVLDLLPRMTYHLDEPIGDPA 347
Query: 341 IYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILA-- 398
+ + ARE G+KV+L G G+DE+ AGY K L +LA
Sbjct: 348 AINTFLICSAAREAGVKVMLSGMGADELFAGYR-----------------KHLANVLALR 390
Query: 399 YKRIHGSLVPLKNMVLYILPVWVTKRLWR-----RRF-----------------PYLREE 436
Y+R+ L + + LPV ++R +R +RF Y R+E
Sbjct: 391 YQRVPRPLRRGVSAAVGRLPVASSRRGYRSVRFAKRFLSFADLPQETAFRRSYTMYDRQE 450
Query: 437 FIEKFKKRPSKEL---------IWKIKTLNE---ALLLAETYYSLPHL-LRFEDKNAMRW 483
++ + + +++ L++ + LA+ LP L L + D+++M
Sbjct: 451 LLDLIDPDLAGTVEDVLTEHADVYEDNDLDDFVNRMCLADARMFLPGLNLAYTDRSSMAA 510
Query: 484 SIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPD 543
S E RVP+ D E+V+ ++P + K+ K +L++ +LP EI R + F+ P
Sbjct: 511 STEVRVPYVDVEVVKAAFAVPGDRKIVGRQGKAVLKEAATSVLPREIVYRPKGL-FSAPL 569
Query: 544 KDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELW 603
+ + + V D E + + + R+ E +G++++S+ LW V+ +E W
Sbjct: 570 RAWMSRDLAPLVREVVNDGE-LVRSGFLRRDALRRLVAEDAAGQRDFSKHLWHVLTLEYW 628
Query: 604 LR 605
R
Sbjct: 629 YR 630
>pir||A83638 probable glutamine amidotransferase PA0051 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9945874|gb|AAG03441.1|AE004445_2 (AE004445) probable
glutamine amidotransferase [Pseudomonas aeruginosa]
Length = 610
Score = 210 bits (529), Expect = 4e-53
Identities = 177/556 (31%), Positives = 269/556 (47%), Gaps = 47/556 (8%)
Query: 1 MCGINGFSWGDEEL------VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCG+ G+ +L + M D + RGPD EG++ N LGH RLA+IDLS G
Sbjct: 1 MCGLAGWVDYTRKLDDEFPAIFAMTDTLALRGPDAEGIWKHRNALLGHRRLAVIDLSG-G 59
Query: 55 HQPMKYE-KDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
QPM Y G+EV +VY GE+YN +R+ L G+ F + +DTEV+L AYL+WG C
Sbjct: 60 VQPMSYRFPTGQEVTLVYTGEVYNHDALRERLRRAGHEFRTRSDTEVVLHAYLQWGERCC 119
Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND 173
E GM+AF ++D L L RDR GIKPLYY + ++F SEIK IL H D
Sbjct: 120 EYLTGMFAFAVFDGRDGHLLLVRDRLGIKPLYYARHREGLLFGSEIKSILAHPEFAARLD 179
Query: 174 AV-IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
AV + D L L T T F ++ L+PG+ + N++ + +Y+++R +
Sbjct: 180 AVGLVDLL--TLSRGTSQTPFREVQELLPGHLLSWRPNSQA-KLRRYWEVRRQEHADDLQ 236
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLP---DGEIKVFSLVF 289
S Q ++L RA+ +L ADVPV S LSGGLDS+++ + + G+I FS+ F
Sbjct: 237 STVQRTRELVTRALGAQLHADVPVCSLLSGGLDSTALTGIAQRIAKAEHGGDINSFSVDF 296
Query: 290 PGK---------EIDESKYQRIVVDECRVSWYRTTF--NVEDVLADI-IDLIRTQEEPFS 337
G+ D+ + ++ + S +RT N E V ++ R ++ PF+
Sbjct: 297 VGQAEQFRSDDLRPDQDQPFALLAAQYIGSRHRTVLIDNAELVCERAREEVFRAKDVPFT 356
Query: 338 TLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREIL 397
+ +M V + G+G+DE+ GY +F V R F W +R L
Sbjct: 357 FGDMDTSLHLMFGEIRRHSTVAISGEGADELFGGYGWFRDPQAVAAAR-FPWASRVR--L 413
Query: 398 AYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLN 457
I +++ Y + R+ +L + E+ + R L L
Sbjct: 414 PAGFIDAGFNRRCDLLQYQQASYDD---GLRQVEHLAGDSPEERRMREFSHL-----HLK 465
Query: 458 EALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVL 517
++L LL +D+ +M +E RVP+ DHELVEYV ++P K G K L
Sbjct: 466 RWMVL---------LLERKDRLSMCNGLEVRVPYTDHELVEYVYNVPWSIKSRDGEEKWL 516
Query: 518 LRKGLKGILPDEIRNR 533
L++ +P+ + R
Sbjct: 517 LKRACADYVPEAVLKR 532
>pir||B83214 probable glutamine amidotransferase PA3459 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9949601|gb|AAG06847.1|AE004766_8 (AE004766) probable
glutamine amidotransferase [Pseudomonas aeruginosa]
Length = 589
Score = 202 bits (509), Expect = 9e-51
Identities = 165/547 (30%), Positives = 254/547 (46%), Gaps = 46/547 (8%)
Query: 1 MCGINGFSWGDEEL-----VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI+G D + V+R+ + RGPD G + ++LGH RL I+DL+
Sbjct: 1 MCGISGEFRFDNQAADLAAVERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLAEASG 60
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVER 115
QPM G + +V+NG IYN+ E+R ELE GY F S DTEV+L Y WG D + R
Sbjct: 61 QPMIDPDLG--LAMVFNGAIYNYPELRGELESLGYRFFSGGDTEVLLKGYHAWGADLLPR 118
Query: 116 FNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAV 175
NGM+AF ++++ + LFL+RDR GIKPLYY D + F+S + +L+ D
Sbjct: 119 LNGMFAFAVWERDRQRLFLARDRLGIKPLYYSLDRSRLRFASSLPALLKGGDIASDLDPQ 178
Query: 176 IFDF-LYYNLLDHTEDTFFEGIKRLMPGYNAVFDLN-TRKFEAWKYYDLRERIEKRKEIS 233
+F L ++ + T EG+K+L P DL+ + + W D R ++R+
Sbjct: 179 ALNFYLNFHAVVPAPHTLLEGVKKLPPATWMSVDLDGSCEQRTWWTLDYGPRPDERELTL 238
Query: 234 D--PQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFP- 290
D + D AV +R A VG LSGG+DSS +V + H + FS+ F
Sbjct: 239 DDWQERVLDGLREAVAIRQRAAREVGVLLSGGVDSSLLV-GLLHEAGVDNLLTFSIGFED 297
Query: 291 --GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVM 348
G+ DE +Y ++ + +R +V+ + R EP + Y
Sbjct: 298 AGGERGDEFQYSDLIAERYHTRHHRLRIGEHEVIEQLPAAFRAMSEPMVSHDCIAFY--- 354
Query: 349 KLARE--NGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSL 406
LARE KV+ GQG+DE+ AGYH++ K AY+
Sbjct: 355 LLAREVSKHCKVVQSGQGADELFAGYHWY-----------PKVDGADDAFAAYRAAFFD- 402
Query: 407 VPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETY 466
++ Y+ V R+ ++R+ F P E +++AL L T
Sbjct: 403 ---RDHEEYLATVGERFRVEDVAGRFVRDHFAS-----PGAE-----AAVDKALRLDSTI 449
Query: 467 YSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGIL 526
+ ++ D M W +E+RVPF D+ L E +P K+ G K +L+ + ++
Sbjct: 450 MLVDDPVKRVDNMTMAWGLEARVPFLDYRLAELSARIPARFKLGDG-GKQVLKGAARKVI 508
Query: 527 PDEIRNR 533
P E+ +R
Sbjct: 509 PSEVIDR 515
>dbj|BAB12569.1| (AB039379) asparagine synthase homolog [Streptomyces aureofaciens]
Length = 609
Score = 201 bits (505), Expect = 3e-50
Identities = 175/580 (30%), Positives = 256/580 (43%), Gaps = 78/580 (13%)
Query: 1 MCGINGFSWGD------EELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI G D +L++ M D + RGPD EG++ LGH RL++ID G
Sbjct: 1 MCGIVGVVDYDAPAEHRRDLLQAMTDTMACRGPDAEGLWFGRAAGLGHRRLSVID-PEHG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QPM E +G+ I ++GEIYNF E+R EL G+ F ++ DTEV+L YL+WG VE
Sbjct: 60 RQPMTAEHEGRGAAITFSGEIYNFRELRAELTSHGHRFRTSCDTEVVLRGYLQWGAALVE 119
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGIL-QHNIPRKPND 173
R NGM+AF ++D+ L L RDR G+KPLYY+ + F SE K +L + R+
Sbjct: 120 RLNGMFAFAVWDEHSEELLLVRDRMGVKPLYYWPTAAGVRFGSEPKAVLADRTLSRRVGP 179
Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNA-VFDLNTRKFEAWKYYDLRERIEKRKEI 232
+ + L +++ E F G+ + PG V R+ W R+E R+
Sbjct: 180 DGLCEVL--DMVKTPEAAVFSGLVEVRPGQLVRVGRGGVRRQTYW-------RLEAREHT 230
Query: 233 SDPQ----GFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLV 288
D +DL V +LV+DVP+G+ LSGGLDSS++ G ++ FS+
Sbjct: 231 DDLPTTIGTVRDLLADTVTRQLVSDVPLGTLLSGGLDSSAVTALAAR---SGPVRSFSVD 287
Query: 289 FPG-----------KEIDESKYQRIV--VDECRVSWYRTTFNVEDVLADIIDLIRTQEEP 335
F G +E D + + V + + + D L T P
Sbjct: 288 FRGAADAFASDAVRREADAPYVRELAGHVGADHLEVLLDSPGLSDPAVRSAVLRATDLPP 347
Query: 336 FSTLSIY-GQYRVMKLARENGMKVLLDGQGSDEILAGYHYF----------FGYYFVELL 384
++ Y + REN + V L G+ +DE+ GY +F F +
Sbjct: 348 AYWGDMWPSLYLFFRAVREN-VTVALSGEAADELFGGYRWFRNPLALRAATFPWLTPGSA 406
Query: 385 RKFKWGKLLREILAYK-RIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK 443
R F L + L K + G L +PV + RR + + +F
Sbjct: 407 RYFGGSSLFQAGLLDKLDLDGYRAARYAEALAEVPVLPGESAPERRMREIGHLNLTRF-- 464
Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
++TL L +D+ +M +E RVPFCDH LVEYV ++
Sbjct: 465 ---------VQTL----------------LDRKDRMSMAVGLEVRVPFCDHRLVEYVFNV 499
Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPD 543
P E K G K LLR + +LP + R AT D
Sbjct: 500 PWEMKSFDGREKSLLRAAVADLLPRSVVERVKTPYPATQD 539
>dbj|BAA89484.1| (AB029550) LtsA [Corynebacterium glutamicum]
Length = 640
Score = 196 bits (494), Expect = 5e-49
Identities = 177/649 (27%), Positives = 293/649 (44%), Gaps = 63/649 (9%)
Query: 1 MCGI------NGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCG+ NG + ++R +RHRGPDD G + D + + G RL+IID++
Sbjct: 1 MCGLLGILTANGNAEAFVPALERALPCMRHRGPDDAGTWHDADAAFGFNRLSIIDIA-HS 59
Query: 55 HQPMKY--EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDC 112
HQP+++ + + +NGEIYN++E+RKEL + GYTF ++ D E I+ + WG
Sbjct: 60 HQPLRWGPADEPDRYAMTFNGEIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHWGESV 119
Query: 113 VERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGIL----QHNIP 168
VE GM+ I+D + LFL+RD+FGIKPL+Y +FSSE K IL + N+
Sbjct: 120 VEHLRGMFGIAIWDTKEKSLFLARDQFGIKPLFYATTEHGTVFSSEKKTILEMAEEMNLD 179
Query: 169 RKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLR---ER 225
+ I ++ + DT I RL G A K E +Y+ + ++
Sbjct: 180 LGLDKRTIEHYVDLQYVPE-PDTLHAQISRLESGCTATVRPG-GKLEQKRYFKPQFPVQK 237
Query: 226 IEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVF 285
+ K KE + +V+ + ADV VGS LSGG+DS++I + PD + F
Sbjct: 238 VVKGKEQDLFDRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAPLAKRHNPD--LLTF 295
Query: 286 SLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQY 345
+ F + E + E+ I ++ ++P + S+ Y
Sbjct: 296 TTGFEREGYSEVDVAAESAAAIGAEHIVKIVSPEEYANAIPKIMWYLDDPVADPSLVPLY 355
Query: 346 RVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGS 405
V AR++ +KV+L G+G+DE+ GY + K + +++I
Sbjct: 356 FVAAEARKH-VKVVLSGEGADELFGGYTIY---------------KEPLSLAPFEKIPSP 399
Query: 406 LVPLKNMVLYILPVWVT-KRLWRRRFPYLREEFIEKFK----KRPSKELIWKIKTLNEAL 460
L + +LP + K L R + E + + ++ + + W + +
Sbjct: 400 LRKGLGKLSKVLPDGMKGKSLLERGSMTMEERYYGNARSFNFEQMQRVIPWAKREWDHRE 459
Query: 461 LLAETYYS---------LPHLLRF----------EDKNAMRWSIESRVPFCDHELVEYVL 501
+ A Y + HL F DK M S+E RVPF D E+ +
Sbjct: 460 VTAPIYAQSRNFDPVARMQHLDLFTWMRGDILVKADKINMANSLELRVPFLDKEVFKVAE 519
Query: 502 SLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVID 561
++P + K++ G TK LR+ L+ I+P + +R K+GF P + L +E F A
Sbjct: 520 TIPYDLKIANGTTKYALRRALEQIVPPHVLHR-KKLGFPVPMRHWLAGDE--LFGWAQDT 576
Query: 562 SESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLREWMDN 610
+ ++ + VL M EH G + S+ LW V+ +W +++N
Sbjct: 577 IKESGTEDIFNKQAVLDMLNEHRDGVSDHSRRLWTVLSFMVWHGIFVEN 625
>gb|AAF17502.1|AF195615_10 (AF195615) PhzH [Pseudomonas chlororaphis]
Length = 610
Score = 192 bits (484), Expect = 7e-48
Identities = 159/575 (27%), Positives = 255/575 (43%), Gaps = 85/575 (14%)
Query: 1 MCGINGFS------WGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCG+ G+ G++ ++ M + + RGPD EG++ + LGH RLA+IDLS G
Sbjct: 1 MCGLTGWVDYTRRLEGEDPAIRAMTNTLALRGPDAEGIWKHRHALLGHRRLAVIDLSG-G 59
Query: 55 HQPMKYE-KDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
QPM Y DG+EV +VY GE+YN +R +L + G+ F + +DTEV+L AYLEWG C
Sbjct: 60 TQPMVYRFADGQEVSLVYTGEVYNHDALRDQLRQAGHVFQTRSDTEVVLHAYLEWGERCC 119
Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND 173
+ GM+AF ++D L L RDR GIKPL+Y + ++F SE K IL H P
Sbjct: 120 DHLTGMFAFALFDGRDGHLLLVRDRLGIKPLFYARHREGLLFGSETKAILAH--PEFTTG 177
Query: 174 AVIFDFL-YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
+ + L T T F + L+PG+ + N + + Y+ +R + +
Sbjct: 178 LEVTGLVDVLTLSKGTAQTPFRNLMELLPGHFLSWRPN-GQMKIQSYWKVRRQEHQHDLQ 236
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL---PDGEIKVFSLVF 289
+ Q ++L A+ +L ADVPV S LSGGLDS+++ + ++ G I FS+ F
Sbjct: 237 TTVQQTRELVTHALGSQLHADVPVCSLLSGGLDSTTLTAIAQRIVKAKTGGNINSFSVDF 296
Query: 290 PGK---------EIDESKYQRIVVDECRVSWYRTTFNVEDVLADII---DLIRTQEEPFS 337
G+ D+ + ++ E S ++T + L + ++ R +E PF+
Sbjct: 297 TGQAEQFKSDDLRPDQDQPFALLAAEFIGSRHQTVLIDNEELVSYLAREEVFRARELPFT 356
Query: 338 TLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREIL 397
+ ++ V + G+G+DE+ GY +F + F W ++
Sbjct: 357 FGDMDTSLNLLFREIRKHSTVAISGEGADEVFGGYGWFRDPKVI-ASASFPWSSRVK--- 412
Query: 398 AYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLN 457
L FI R ++ + +
Sbjct: 413 -----------------------------------LPAGFINADFNRQCDLAQYQQASYD 437
Query: 458 EALLLAE-TYYSLPHLLRFED---KNAMRWSI-----ESRVPFC----------DHELVE 498
+ALL E PH R + + RW + + R+ C DHELVE
Sbjct: 438 DALLQVEHLAQDSPHERRMRELCHMHLKRWMVMLLDRKDRLSMCNSLEVRVPFTDHELVE 497
Query: 499 YVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNR 533
Y+ ++P K G K LL++ +P+ + R
Sbjct: 498 YIYNVPWSIKSKDGEEKWLLKQACADFVPEAVLKR 532
>sp|Q10374|ASNH_MYCTU PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]
>gi|7477893|pir||B70785 probable asparagine synthetase -
Mycobacterium tuberculosis (strain H37RV)
>gi|1237054|emb|CAA94270.1| (Z70283) asnB [Mycobacterium
tuberculosis]
Length = 652
Score = 180 bits (452), Expect = 4e-44
Identities = 172/623 (27%), Positives = 286/623 (45%), Gaps = 52/623 (8%)
Query: 15 VKRMNDAIRHRGPDDEGVY-----VDDNVSLGHVRLAIIDLSPKGHQPMKY---EKDGKE 66
+ R + +RHRGPD+ G + V G RL+IID++ HQP+++ E +
Sbjct: 26 IARASHLMRHRGPDESGTWHAVDGASGGVVFGFNRLSIIDIA-HSHQPLRWGPPEAPDRY 84
Query: 67 VWIVYNGEIYNFMEIRKELE-EKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIY 125
V +V+NGEIYN++E+R EL + G F ++ D E ILA Y WG + ++R GM+AF ++
Sbjct: 85 V-LVFNGEIYNYLELRDELRTQHGAVFATDGDGEAILAGYHHWGTEVLQRLRGMFAFALW 143
Query: 126 DKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFD------- 178
D LF +RD FGIKPL+ +SE K +L + V FD
Sbjct: 144 DTVTRELFCARDPFGIKPLFIATGAGGTAVASEKKCLLD------LVELVGFDTEIDHRA 197
Query: 179 FLYYNLLDHT--EDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQ 236
+Y +L + +T G++RL G A + +Y+ R +D
Sbjct: 198 LQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRYFVPRFAASPITNDNDQA 257
Query: 237 GFKDL---FLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVFPGK 292
+ ++ +V + ADV VG+ LSGG+DS++I A+RH + + F+ F +
Sbjct: 258 RYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRH---NPRLITFTTGFERE 314
Query: 293 EIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLAR 352
E + + ++ +A + +++ +EP + ++ + V + AR
Sbjct: 315 GFSEIDVAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEPVADPALVPLFFVAREAR 374
Query: 353 ENGMKVLLDGQGSDEILAGYHYF--------FGYYFVELLRKF-KWGKLLREILAYKR-I 402
++ +KV+L G+G+DE+ GY + F Y L R K K L E + K +
Sbjct: 375 KH-VKVVLSGEGADELFGGYTIYREPLSLRPFDYLPKPLRRSMGKVSKPLPEGMRGKSLL 433
Query: 403 HGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRP--SKELIWKIKTLNEAL 460
H + L+ + +L R P R ++ P ++ W + +
Sbjct: 434 HRGSLTLEERYYGNARSFSGAQL-REVLPGFRPDWTHTDVTAPVYAESAGWDPVARMQHI 492
Query: 461 LLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRK 520
L + +L DK M S+E RVPF D E+ LP +K++ TK LR+
Sbjct: 493 DLFT--WLRGDILVKADKITMANSLELRVPFLDPEVFAVASRLPAGAKITRTTTKYALRR 550
Query: 521 GLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMF 580
L+ I+P + +R K+GF P + L+ E ++A A + S + D V RM
Sbjct: 551 ALEPIVPAHVLHR-PKLGFPVPIRHWLRAGELLEWAYATVGSS--QAGHLVDIAAVYRML 607
Query: 581 EEHVSGKKNWSQELWKVIIMELW 603
+EH G + S+ LW ++I LW
Sbjct: 608 DEHRCGSSDHSRRLWTMLIFMLW 630
>gb|AAG02363.1|AF210249_22 (AF210249) asparagine synthetase [Streptomyces verticillus]
Length = 638
Score = 176 bits (441), Expect = 8e-43
Identities = 176/575 (30%), Positives = 256/575 (43%), Gaps = 55/575 (9%)
Query: 1 MCGINGFSWGDE-----ELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPK 53
+CGI D EL M D +R RGPD EG +V +LGH RLA+I
Sbjct: 4 VCGIVAIRSADGGLDGGELTAPMAD-LRPRGPDGEGTWVSPTGRAALGHTRLAVI-APDA 61
Query: 54 GHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
G QP+ DG V +V NGE Y + EIR EL G F + +D+E+ L YL G +
Sbjct: 62 GRQPVA-GPDGT-VRLVVNGEFYGYREIRAELRAAGCRFRTGSDSEIALHLYLRDGRRAL 119
Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND 173
ER G +AFV++D+ + LF +RDRFG+KPLYY + +S ++ +L P + +
Sbjct: 120 ERLRGEFAFVLWDERRATLFAARDRFGVKPLYYTERDGRLYVASTVRALLSCGAPARWDT 179
Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLN-TRKFEAWKY-YDLRERIEKRKE 231
A L L + T F GI++L PG + + D + TR W Y + R
Sbjct: 180 AAFAAHLQLGL--PPDRTLFAGIRQLPPGCHLIADAHGTRVTPYWDLDYPPAGELAARGS 237
Query: 232 ISDP-QGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVF 289
+ D ++ AV++R VADVP+ LSGGLDSS++ A RH + F++ F
Sbjct: 238 LDDHLDAVRERTDEAVRLRTVADVPLACHLSGGLDSSAVAASAARHT----RLTAFTVRF 293
Query: 290 PGKEIDESKYQR-----IVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
DES R + +D V+ R F D L D++ +E ++ Y
Sbjct: 294 DDPAFDESAVARRTAAHLAIDHREVASERAHF--ADHLRDVVRAGEMVQENSHGIARYLH 351
Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRI-- 402
+K A G +L G+G DE+ GY F + L + K R Y R+
Sbjct: 352 SAHIKKA---GFTAVLAGEGGDELFLGYPQFRKDLTLSLSADAR-DKADR---GYARLVA 404
Query: 403 HGSLVP-LKNMV--LYILPVWVTKRLWRRRFPY---LREEFIEKFKKRPSKE-------- 448
G L P L+ ++ L LP W+ R P LR +F + + +
Sbjct: 405 AGLLPPYLRTLLGTLGFLPSWIVDRHLAVTQPVAALLRPDFAAELARADAAAPLLAAGAG 464
Query: 449 -LIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPES 507
L + L A+T+ LP L ++ ++E R+P DH L + V PP
Sbjct: 465 LLAGRAPAHQATYLFAKTW--LPGYLLAAERLDAAQAVEVRLPLFDHHLFDLVRHTPPAW 522
Query: 508 KVSAGITKVLLRKGLKGILPDEIRNRASKVGFATP 542
G K LR ++ LP E+ K GF P
Sbjct: 523 YDKDGTGKYPLRAAMRHRLPREV-TEGRKQGFLAP 556
>sp|P42113|ASNH_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2
>gi|7474448|pir||A69591 asparagine synthetase asnH -
Bacillus subtilis >gi|904192|dbj|BAA21593.1| (AB005554)
probable asparagine synthetase [Bacillus subtilis]
>gi|2636538|emb|CAB16028.1| (Z99124) asparagine
synthetase (glutamine-hydrolyzing) [Bacillus subtilis]
Length = 747
Score = 170 bits (427), Expect = 4e-41
Identities = 162/547 (29%), Positives = 258/547 (46%), Gaps = 66/547 (12%)
Query: 14 LVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNG 73
++K M DAI +RGPDDE ++D V RL+I+DL G QP E DG V ++ NG
Sbjct: 22 ILKGMADAISYRGPDDEQYHIDSKVGFAFRRLSILDLV-NGQQPFLNE-DGSIV-VMVNG 78
Query: 74 EIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILF 133
EIYN+ E++ L + F + +D EVI+ Y E G V+ GM++ I+DK+KN +F
Sbjct: 79 EIYNYKELKASLHN--HMFKTTSDCEVIVHLYEEKGIGFVDDIIGMFSIAIWDKNKNKVF 136
Query: 134 LSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPNDAVIFDFLYYN---LLDHTE 189
L RDRFGIKPL+Y +IF+SEIK + H + PR+ N + + +H E
Sbjct: 137 LVRDRFGIKPLFYTELKHELIFASEIKSLFSHPHCPRQFNWKEALSDICLSGEAASNHKE 196
Query: 190 DT-FFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKR--KEISDP----QGFKDLF 242
T FF I+ L G+ +L T + + Y+ L++ + ++ +E P +G+++L
Sbjct: 197 TTSFFVNIQNLDAGHYLEINLTTNERKTASYWSLQDILLRQGYRENLHPDDLIEGYRELL 256
Query: 243 LRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDE-SKYQR 301
+V L +DV VG LSGG+DS+++ ++ F+++ +E +KY
Sbjct: 257 ADSVHRCLQSDVEVGLFLSGGIDSAAVA---HFAAEKQDLHTFTVLSQSTFTNEDAKYAH 313
Query: 302 IVVDECRVSWYRTTFNV--EDVL--ADIIDLIRTQEEPF---STLSIYGQYRVMKLAREN 354
+ + + ++ + + +++L L+ E PF L + ++ K R N
Sbjct: 314 WLAKDLHLPNHQVLYQLGNDELLQPESYKHLLWICETPFCGPEQLYKFHLHKYAKAIRPN 373
Query: 355 GMKVLLDGQGSDEILAGYHYFFG-------YYFVELLRKFKWGKLLREILAYKRIHGSLV 407
+KV+L GQGSDE GY F+E + + +L R+ G++
Sbjct: 374 -LKVMLTGQGSDEFNGGYSTTLSPAENPSWEGFIESVNTMEMNRL-------HRLQGNIF 425
Query: 408 PLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKEL-IWKIKTLNEALLLAETY 466
R+W F L + K S + W+ L + L
Sbjct: 426 ----------------RVWEEHFG-LSPINLSYLKSNDSSQADPWQSYVLTKYRDL--QM 466
Query: 467 YSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGIL 526
Y+ H ED+ A IE+RVPF DH LVE+V +P + K +LRK L L
Sbjct: 467 YNCWH----EDRIAAANHIENRVPFLDHRLVEWVCGIPDGLRKDLLWDKSVLRKSLTNEL 522
Query: 527 PDEIRNR 533
+R
Sbjct: 523 HTSYTHR 529
>pir||H82255 asparagine synthetase B, glutamine-hydrolyzing VC0991 [imported] -
Vibrio cholerae (group O1 strain N16961)
>gi|9655452|gb|AAF94152.1| (AE004180) asparagine
synthetase B, glutamine-hydrolyzing [Vibrio cholerae]
Length = 554
Score = 143 bits (358), Expect = 4e-33
Identities = 119/375 (31%), Positives = 179/375 (47%), Gaps = 54/375 (14%)
Query: 18 MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYN 77
M+ +RHRGPD G+Y D L H RLAI+ L+ G QP+ Y D K + V NGEIYN
Sbjct: 24 MSKKLRHRGPDWSGIYASDKAILAHERLAIVGLN-SGAQPL-YSPDRKLILAV-NGEIYN 80
Query: 78 FMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRD 137
EIR+ + + Y F +++D EVILA Y + G D +E NG++AFV+YD+ K+ + RD
Sbjct: 81 HKEIRERYKGQ-YEFQTDSDCEVILALYRDKGADLLEELNGIFAFVLYDEEKDEYLIGRD 139
Query: 138 RFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGI 196
GI PLY +D N +SE+K ++ P + +F + L +
Sbjct: 140 HIGIIPLYQGHDEHGNFYVASEMKALV-------PVCKTLSEFPPGSYLSSKDQAV---- 188
Query: 197 KRLMPGYNAVFDLNTRKF-EAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVP 255
TR + W +D E + +S + AVK +L+ DVP
Sbjct: 189 --------------TRYYVRDWNSFD-----EVKDNVSSKEELTQALEAAVKRQLMTDVP 229
Query: 256 VGSCLSGGLDSS------SIVCAMRHLLPDGE------IKVFSLVFPGKEIDESKYQRIV 303
G LSGGLDSS AMR + D E ++ S + + K R V
Sbjct: 230 YGVLLSGGLDSSITSAIAKRFAAMR--IEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREV 287
Query: 304 VDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLL 360
++ + T+ +++ L I D+I E + +I + + R+ G+K++L
Sbjct: 288 AEKIGTVHHEMTYTIQEGLDAIRDVI-YHIETYDVTTIRASTPMFLMGRKIKAMGIKMVL 346
Query: 361 DGQGSDEILAGYHYF 375
G+G+DEI GY YF
Sbjct: 347 SGEGADEIFGGYLYF 361
>sp|P22106|ASNB_ECOLI ASPARAGINE SYNTHETASE B [GLUTAMINE-HYDROLYZING]
>gi|68659|pir||AJECN asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) - Escherichia coli
>gi|145393|gb|AAA23498.1| (J05554) asparagine synthetase
B [Escherichia coli] >gi|1651277|dbj|BAA35317.1|
(D90706) Asparagine synthase (glutamine-hydrolyzing) (EC
6.3.5.4) [Escherichia coli] >gi|1786889|gb|AAC73768.1|
(AE000171) asparagine synthetase B [Escherichia coli
K12]
Length = 554
Score = 139 bits (346), Expect = 1e-31
Identities = 119/370 (32%), Positives = 175/370 (47%), Gaps = 52/370 (14%)
Query: 22 IRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEI 81
+RHRGPD G+Y DN L H RL+I+D++ G QP+ Y + V V NGEIYN +
Sbjct: 28 MRHRGPDWSGIYASDNAILAHERLSIVDVNA-GAQPL-YNQQKTHVLAV-NGEIYNHQAL 84
Query: 82 RKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGI 141
R E ++ Y F + +D EVILA Y E G + ++ GM+AF +YD K+ + RD GI
Sbjct: 85 RAEYGDR-YQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGI 143
Query: 142 KPLYYYYDGKNIIF-SSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLM 200
PLY YD ++ +SE+K ++ P I +F + L + R
Sbjct: 144 IPLYMGYDEHGQLYVASEMKALV-------PVCRTIKEFPAGSYLWSQDGEIRSYYHRDW 196
Query: 201 PGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCL 260
Y+AV D T K E LR+ +E +VK L++DVP G L
Sbjct: 197 FDYDAVKDNVTDKNE------LRQALED----------------SVKSHLMSDVPYGVLL 234
Query: 261 SGGLDSSSIVCAMR----HLLPDGE--------IKVFSLVFPGKEIDESKYQRIVVDECR 308
SGGLDSS I + + D E + F++ PG + K + V +
Sbjct: 235 SGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGS--PDLKAAQEVANHLG 292
Query: 309 VSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGS 365
+ F V+ D + D+I I T + S Y + + + G+K++L G+GS
Sbjct: 293 TVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRAST-PMYLMSRKIKAMGIKMVLSGEGS 351
Query: 366 DEILAGYHYF 375
DE+ GY YF
Sbjct: 352 DEVFGGYLYF 361
>pdb|1CT9|A Chain A, Crystal Structure Of Asparagine Synthetase B From
Escherichia Coli >gi|6730196|pdb|1CT9|B Chain B, Crystal
Structure Of Asparagine Synthetase B From Escherichia
Coli >gi|6730197|pdb|1CT9|C Chain C, Crystal Structure
Of Asparagine Synthetase B From Escherichia Coli
>gi|6730198|pdb|1CT9|D Chain D, Crystal Structure Of
Asparagine Synthetase B From Escherichia Coli
Length = 553
Score = 139 bits (346), Expect = 1e-31
Identities = 119/370 (32%), Positives = 175/370 (47%), Gaps = 52/370 (14%)
Query: 22 IRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEI 81
+RHRGPD G+Y DN L H RL+I+D++ G QP+ Y + V V NGEIYN +
Sbjct: 27 MRHRGPDWSGIYASDNAILAHERLSIVDVNA-GAQPL-YNQQKTHVLAV-NGEIYNHQAL 83
Query: 82 RKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGI 141
R E ++ Y F + +D EVILA Y E G + ++ GM+AF +YD K+ + RD GI
Sbjct: 84 RAEYGDR-YQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGI 142
Query: 142 KPLYYYYDGKNIIF-SSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLM 200
PLY YD ++ +SE+K ++ P I +F + L + R
Sbjct: 143 IPLYMGYDEHGQLYVASEMKALV-------PVCRTIKEFPAGSYLWSQDGEIRSYYHRDW 195
Query: 201 PGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCL 260
Y+AV D T K E LR+ +E +VK L++DVP G L
Sbjct: 196 FDYDAVKDNVTDKNE------LRQALED----------------SVKSHLMSDVPYGVLL 233
Query: 261 SGGLDSSSIVCAMR----HLLPDGE--------IKVFSLVFPGKEIDESKYQRIVVDECR 308
SGGLDSS I + + D E + F++ PG + K + V +
Sbjct: 234 SGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGS--PDLKAAQEVANHLG 291
Query: 309 VSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGS 365
+ F V+ D + D+I I T + S Y + + + G+K++L G+GS
Sbjct: 292 TVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRAST-PMYLMSRKIKAMGIKMVLSGEGS 350
Query: 366 DEILAGYHYF 375
DE+ GY YF
Sbjct: 351 DEVFGGYLYF 360
>dbj|BAA89376.1| (AB025342) ORF2 [Moritella marina]
Length = 555
Score = 137 bits (342), Expect = 3e-31
Identities = 120/379 (31%), Positives = 180/379 (46%), Gaps = 52/379 (13%)
Query: 13 ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYN 72
E ++ +RHRGPD G+Y DN L H RLAI+D++ G QP+ E+ K + N
Sbjct: 19 EQALELSKLLRHRGPDWSGIYTSDNAILVHERLAIVDVN-NGAQPLYNEE--KTHVLAVN 75
Query: 73 GEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNIL 132
GEIYN +++K L + F + +D EVILA Y E G ++ NG++AF +YD++++
Sbjct: 76 GEIYNHKDLKKTLNVD-FEFQTESDCEVILALYKEKGTQFLDDLNGIFAFALYDETEDAY 134
Query: 133 FLSRDRFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDT 191
+ RD GI PLY YD N +SE+K ++ P I +F + L +
Sbjct: 135 LIGRDHIGIIPLYTGYDEHGNFYVASEMKALV-------PICTQIEEFPAGHYLWSKDGV 187
Query: 192 FFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLV 251
+ P Y WK +D + K + QG +D AVK +L+
Sbjct: 188 -------VTPYYQ----------RDWKDFDNVAQNGGDKSVV-KQGLED----AVKRQLM 225
Query: 252 ADVPVGSCLSGGLDSSSIVCA----MRHLLPDG--------EIKVFSLVFPGKEIDESKY 299
DVP G LSGGLDSS I + + DG ++ FS+ G D +
Sbjct: 226 CDVPYGVLLSGGLDSSVISAITQQYAKRRIEDGGKTEAWWPQLHSFSVGLNGSP-DLAAA 284
Query: 300 QRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGM 356
Q+ V D + F V+ D L D+I I T + S Y + + + G+
Sbjct: 285 QK-VADHLGTIHHSIEFTVQEGIDALRDVIYHIETYDVTTIRAST-PMYLMARKIKAMGI 342
Query: 357 KVLLDGQGSDEILAGYHYF 375
K++L G+G+DE+ GY YF
Sbjct: 343 KMVLSGEGADELFGGYLYF 361
>gb|AAB99117.1| (U67554) asparagine synthetase (asnB) [Methanococcus jannaschii]
Length = 544
Score = 136 bits (338), Expect = 1e-30
Identities = 120/409 (29%), Positives = 195/409 (47%), Gaps = 72/409 (17%)
Query: 1 MCGINGFSWGDEELVKR----MNDAIRHRGPDDEGVYVDD-------------------- 36
MC I+G D ++ + M ++HRG D+ G+ +DD
Sbjct: 4 MCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIG 63
Query: 37 NVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNT 96
N+SL H RLAI+ G QP+ E + +W+V NGEIYN++E+R+ L++ + F +++
Sbjct: 64 NLSLAHNRLAIV--GRYGVQPIPNEDE--TIWLVCNGEIYNYIELREYLKQN-HEFRTDS 118
Query: 97 DTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFS 156
D EVI+ Y E + +E +G +AF IYDKSKN++ L+RD FG+KPL+Y K F+
Sbjct: 119 DNEVIIHLYEE---EKLEELDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVDRDKYFAFA 175
Query: 157 SEIKGI--LQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKF 214
SE K + L NI D D IK L P ++ L+ +F
Sbjct: 176 SERKALWHLLINIDGCERDL---------------DELNSKIKTLKPNSQLIYYLDDNRF 220
Query: 215 EAWK-YYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADV-PVGSCLSGGLDSS----- 267
E + + L K + + + + D L+ ++ V + VG SGG+DSS
Sbjct: 221 EIIEGFKKLELNYMKERSYEEAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKL 280
Query: 268 -SIVCAMRHLLPDGEIKVFSLVFP---GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLA 323
S+ C + L G L++ K+++ ++I+ +E + F V +
Sbjct: 281 ASLYCEV-ILYAVGTENSEDLIYAERLAKDLNLKLRKKIISEE---EYEEYVFKVAKAI- 335
Query: 324 DIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
D +DL++ Y ++A E+G+KV+L GQG+DE+ GY
Sbjct: 336 DEVDLMKIGVG-------IPIYVASEMANEDGLKVVLSGQGADELFGGY 377
>sp|Q58516|ASNH_METJA PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]
>gi|11272681|pir||C64439 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) MJ1116 [similarity]
- Methanococcus jannaschii
Length = 541
Score = 136 bits (338), Expect = 1e-30
Identities = 120/409 (29%), Positives = 195/409 (47%), Gaps = 72/409 (17%)
Query: 1 MCGINGFSWGDEELVKR----MNDAIRHRGPDDEGVYVDD-------------------- 36
MC I+G D ++ + M ++HRG D+ G+ +DD
Sbjct: 1 MCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIG 60
Query: 37 NVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNT 96
N+SL H RLAI+ G QP+ E + +W+V NGEIYN++E+R+ L++ + F +++
Sbjct: 61 NLSLAHNRLAIV--GRYGVQPIPNEDE--TIWLVCNGEIYNYIELREYLKQN-HEFRTDS 115
Query: 97 DTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFS 156
D EVI+ Y E + +E +G +AF IYDKSKN++ L+RD FG+KPL+Y K F+
Sbjct: 116 DNEVIIHLYEE---EKLEELDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVDRDKYFAFA 172
Query: 157 SEIKGI--LQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKF 214
SE K + L NI D D IK L P ++ L+ +F
Sbjct: 173 SERKALWHLLINIDGCERDL---------------DELNSKIKTLKPNSQLIYYLDDNRF 217
Query: 215 EAWK-YYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADV-PVGSCLSGGLDSS----- 267
E + + L K + + + + D L+ ++ V + VG SGG+DSS
Sbjct: 218 EIIEGFKKLELNYMKERSYEEAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKL 277
Query: 268 -SIVCAMRHLLPDGEIKVFSLVFP---GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLA 323
S+ C + L G L++ K+++ ++I+ +E + F V +
Sbjct: 278 ASLYCEV-ILYAVGTENSEDLIYAERLAKDLNLKLRKKIISEE---EYEEYVFKVAKAI- 332
Query: 324 DIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
D +DL++ Y ++A E+G+KV+L GQG+DE+ GY
Sbjct: 333 DEVDLMKIGVG-------IPIYVASEMANEDGLKVVLSGQGADELFGGY 374
>emb|CAA96526.1| (Z72354) asparagine synthetase [Vicia faba]
Length = 586
Score = 135 bits (336), Expect = 2e-30
Identities = 115/391 (29%), Positives = 181/391 (45%), Gaps = 51/391 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+RK+L +TF + D +VI Y E+G + V+
Sbjct: 60 DQPLFNED--KSIIVTVNGEIYNHEELRKQLPN--HTFRTQCDCDVIAHLYEEYGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ +SE+KG+ N
Sbjct: 116 LLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ R+F W
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKEREFRRWYNPPWFNEAIIPSTP 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
DP + F +AV RL+ DVP G LSGGLDSS + L D + K+ S
Sbjct: 206 YDPLVLRTAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLADTKAAKQWGAKLHS 265
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
K + K + V D + F ++D + I D+I T+ +T+ +
Sbjct: 266 FCVGLKGAPDLKAGKEVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325
Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ + + + +G+K+L+ G+GSDEI GY YF
Sbjct: 326 FLMSRKIKSSGVKMLISGEGSDEIFGGYLYF 356
>gb|AAF02775.1|AF190728_1 (AF190728) asparagine synthetase [Helianthus annuus]
Length = 591
Score = 133 bits (332), Expect = 5e-30
Identities = 122/394 (30%), Positives = 177/394 (43%), Gaps = 56/394 (14%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G+Y + L H RLAIID + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHGDNYLSHQRLAIIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E + + + NGEIYN E+RK L G+ F + +D +VI Y E G D V+
Sbjct: 60 DQPLFNED--QTIVVTVNGEIYNHEELRKRLTAAGHKFRTGSDCDVIAHLYEEHGEDFVD 117
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM++FV+ D N +RD GI LY + DG ++ SSE+KG+ N
Sbjct: 118 MLDGMFSFVLLDTRNNTYIAARDAIGITSLYIGWGLDG-SVWISSELKGL---------N 167
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ T F+ W Y L
Sbjct: 168 DDC------------------EHFEVFPPGH--LYSSKTGGFKRW-YNPLWFAETIPSTP 206
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLP--------DGEIKV 284
DP + F AV RL+ DVP G LSGGLDSS + L ++
Sbjct: 207 YDPLVLRCAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTKAAKQWGAQLHS 266
Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
F + G + K R V D + F V+D + I D+I E + +I
Sbjct: 267 FCVGLEGS--PDLKAAREVADYLGTVHHEFHFTVQDGIDAIEDVI-YHIETYDVTTIRAS 323
Query: 345 YRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K+++ G+GSDEI GY YF
Sbjct: 324 TPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 357
>dbj|BAA96252.1| (AB035248) asparagine synthetase [Astragalus sinicus]
Length = 586
Score = 133 bits (332), Expect = 5e-30
Identities = 117/395 (29%), Positives = 186/395 (46%), Gaps = 60/395 (15%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ ++ K + + NGEIYN E+RK+L + F ++ D +VI Y E+G + V+
Sbjct: 60 DQPLFNQE--KSIIVTVNGEIYNHEELRKQLPN--HKFRTDCDCDVIAHLYEEYGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ +SE+KG+ N
Sbjct: 116 MLDGIFSFVVLDTRDNSFLVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
D E + PG+ ++ R+F W K++ E +
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKEREFRRWYNPKWFS--ETVPSS 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IK 283
DP + F +AV RL++DVP G LSGGLDSS + L D + K
Sbjct: 204 P--YDPLVLRHAFEKAVIKRLMSDVPFGVLLSGGLDSSLVASVTARYLEDTKAAKQWGAK 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
+ + K + K R V D + F ++D + I D+I E F +I
Sbjct: 262 LHTFCVGLKGAPDLKAGREVADYLGTVHHEFEFTIQDGIDAIEDVI-YHTETFDVTTIRA 320
Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEI GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355
>gb|AAF02776.1|AF190729_1 (AF190729) asparagine synthetase [Helianthus annuus]
Length = 558
Score = 133 bits (331), Expect = 6e-30
Identities = 120/394 (30%), Positives = 180/394 (45%), Gaps = 58/394 (14%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI F D KR ++ +RHRGPD G++ +++ L H RLAI+D + G
Sbjct: 1 MCGILAVFGCVDCSQAKRARILELSSRLRHRGPDWSGLHSEEDCYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K + + NGEIYN E R++L K + F + +D EVI Y E G D V
Sbjct: 60 DQPL-YNED-KSIIVTVNGEIYNHKEFREQL--KSHKFNTGSDCEVIAHLYEEHGEDFVH 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM++FV+ D +RD GI PLY + DG ++ F+SE+K +
Sbjct: 116 MLDGMFSFVLLDTRNKSYIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
++D E +PG+ ++ W +
Sbjct: 165 ---------------SDDC--EQFMSFLPGH--IYSSKNGGLRRWYNPPWWSELVPSTPY 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GEIKV 284
DP ++ F +AV RL+ DVP G LSGGLDSS + A RHL+ ++
Sbjct: 206 -DPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVASRHLVDSEAYCQWGSQLHT 264
Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSI 341
F + G + R V D + F V+ D L ++I I T + S
Sbjct: 265 FCIGLKGS--PDLVAAREVADYLGTRHHEFYFTVQEGIDALEEVIYHIETYDVTTIRAST 322
Query: 342 YGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ + + + G+K++L G+GSDEI GY YF
Sbjct: 323 -PMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355
>dbj|BAA97313.1| (AB019236) asparagine synthetase [Arabidopsis thaliana]
Length = 578
Score = 132 bits (329), Expect = 1e-29
Identities = 120/394 (30%), Positives = 182/394 (45%), Gaps = 58/394 (14%)
Query: 1 MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI S + ++ +RHRGPD G++ ++ L H RLAIID + G
Sbjct: 1 MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPT-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K V + NGEIYN +R++L K + F + +D EVI Y E G + ++
Sbjct: 60 DQPL-YNED-KTVAVTVNGEIYNHKILREKL--KSHQFRTGSDCEVIAHLYEEHGEEFID 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM+AFV+ D +RD GI PLY + DG ++ F+SE+K +
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
++D E PG+ ++ W Y +
Sbjct: 165 ---------------SDDC--EQFMSFPPGH--IYSSKQGGLRRW-YNPPWYNEQVPSTP 204
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GEIKV 284
DP ++ F +AV RL+ DVP G LSGGLDSS + A+RHL ++
Sbjct: 205 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHT 264
Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
F + G + K R V D + F V+D + D I+ + E + +I
Sbjct: 265 FCIGLQGS--PDLKAGREVADYLGTRHHEFQFTVQDGI-DAIEEVIYHIETYDVTTIRAS 321
Query: 345 YRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEIL GY YF
Sbjct: 322 TPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 355
>gb|AAC72837.1| (AF095453) asparagine synthetase [Arabidopsis thaliana]
Length = 578
Score = 132 bits (329), Expect = 1e-29
Identities = 120/394 (30%), Positives = 182/394 (45%), Gaps = 58/394 (14%)
Query: 1 MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI S + ++ +RHRGPD G++ ++ L H RLAIID + G
Sbjct: 1 MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPT-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K V + NGEIYN +R++L K + F + +D EVI Y E G + ++
Sbjct: 60 DQPL-YNED-KTVAVTVNGEIYNHKILREKL--KSHQFRTGSDCEVIAHLYEEHGEEFID 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM+AFV+ D +RD GI PLY + DG ++ F+SE+K +
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
++D E PG+ ++ W Y +
Sbjct: 165 ---------------SDDC--EQFMSFPPGH--IYSSKQGGLRRW-YNPPWYNEQVPSTP 204
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GEIKV 284
DP ++ F +AV RL+ DVP G LSGGLDSS + A+RHL ++
Sbjct: 205 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHT 264
Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
F + G + K R V D + F V+D + D I+ + E + +I
Sbjct: 265 FCIGLQGS--PDLKAGREVADYLGTRHHEFQFTVQDGI-DAIEEVIYHIETYDVTTIRAS 321
Query: 345 YRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEIL GY YF
Sbjct: 322 TPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 355
>gi|4502259 asparagine synthetase [Homo sapiens]
>gi|114266|sp|P08243|ASNS_HUMAN ASPARAGINE SYNTHETASE
[GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT ASPARAGINE
SYNTHETASE) (TS11 CELL CYCLE CONTROL PROTEIN)
>gi|68655|pir||AJHUN1 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) - human
>gi|179100|gb|AAA51789.1| (M27396) asparagine synthetase
[Homo sapiens] >gi|533200|gb|AAA52756.1| (L35946)
asparagine synthetase [Homo sapiens]
Length = 561
Score = 131 bits (326), Expect = 2e-29
Identities = 160/624 (25%), Positives = 255/624 (40%), Gaps = 116/624 (18%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI D+ L + A++ HRGPD E V N G RLA++D G
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
QP++ +K +W+ YNGEIYN ++++ E + + + D E+IL Y + G + +
Sbjct: 60 QPIRVKKY-PYLWLCYNGEIYNHKKMQQHFE---FEYQTKVDGEIILHLYDKGGIEQTIC 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
+G++AFV+ D + +FL RD +G++PL+ + SE KG++
Sbjct: 116 MLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVT--------- 166
Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
L H+ F + ++ +PG+ V DL E KY
Sbjct: 167 -----------LKHSATPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHA 214
Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
YD E++ EI + + LF AVK RL+ D +G LSGGLDSS + + L
Sbjct: 215 LYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQL 274
Query: 278 PDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIIDLIRTQ 332
+ +++ + + ++ + R V D Y FN E+ L ++I + T
Sbjct: 275 KEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY 334
Query: 333 EEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGK 391
+ S+ G Y + K R+N V+ G+GSDE+ GY YF E + + +
Sbjct: 335 DITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESER 392
Query: 392 LLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIW 451
LLRE+ + + + + R P+L F + P + I
Sbjct: 393 LLRELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFFSYYLSLPPEMRI- 438
Query: 452 KIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSA 511
K E LL ET FED N L+ + P+ S
Sbjct: 439 -PKNGIEKHLLRET---------FEDSN----------------LIPKEILWRPKEAFSD 472
Query: 512 GITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYW 571
GIT V + IL + + ++ D + N QKF + K + +
Sbjct: 473 GITSV--KNSWFKILQEYVEHQVD---------DAMMANAAQKFPF-----NTPKTKEGY 516
Query: 572 DYRKVLRMFEEHVSGKKNWSQELW 595
YR+V FE H G+ +W W
Sbjct: 517 YYRQV---FERHYPGRADWLSHYW 537
>sp|O24661|ASNS_TRIVS ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|2429280|gb|AAD05033.1|
(AF014055) asparagine synthetase [Triphysaria
versicolor] >gi|2429282|gb|AAD05034.1| (AF014056)
asparagine synthetase [Triphysaria versicolor]
>gi|2429284|gb|AAD05035.1| (AF014057) asparagine
synthetase [Triphysaria versicolor]
Length = 586
Score = 131 bits (325), Expect = 3e-29
Identities = 124/395 (31%), Positives = 180/395 (45%), Gaps = 60/395 (15%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGIHHHGDCYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+R L + F + +D +VI Y E+G + VE
Sbjct: 60 DQPLFNED--KRIAVTVNGEIYNHEELRALLPN--HKFRTGSDCDVIAHLYEEYGENFVE 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM++FV+ D N +RD FGI LY + DG ++ SSE+KG+
Sbjct: 116 MLDGMFSFVLLDSRDNTFIAARDAFGITSLYIGWGLDG-SVWISSELKGL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
H E FE PG+ V+ T F W Y+ E I
Sbjct: 165 --------------HDECENFEVFP---PGH--VYSSKTEGFRRW--YNPPWFSEAIPST 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIK----- 283
DP + F +AV RL+ DVP G LSGGLDSS + RHL K
Sbjct: 204 P--YDPLVLRGAFEQAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGTKAAKRWGSQ 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
+ S + + K + V D + F V+D + I D+I E + +I
Sbjct: 262 LHSFCVGLEGSPDLKAGKEVADYLGTVHHEFLFTVQDGIDAIEDVI-YHIETYDVTTIRA 320
Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K+++ G+GSDEI GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355
>gi|11421677 asparagine synthetase [Homo sapiens] >gi|3341715|gb|AAC62263.1|
(AC005326) asparagine synthetase [Homo sapiens]
Length = 561
Score = 130 bits (324), Expect = 4e-29
Identities = 161/624 (25%), Positives = 255/624 (40%), Gaps = 116/624 (18%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI D+ L + A++ HRGPD E V N G RLA++D G
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
QP++ +K +W+ YNGEIYN ++++ E + + + D E+IL Y + G + +
Sbjct: 60 QPIRVKKY-PYLWLCYNGEIYNHKKMQQHFE---FEYQTKVDGEIILHLYDKGGIEQTIC 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
+G++AFV+ D + +FL RD +G++PL+ + SE KG++
Sbjct: 116 MLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVT--------- 166
Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
L H+ F + ++ +PG+ V DL E KY
Sbjct: 167 -----------LKHSATPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHA 214
Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
YD E++ EI + + LF AVK RL+ D +G LSGGLDSS + + L
Sbjct: 215 LYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQL 274
Query: 278 PDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIIDLIRTQ 332
+ +++ + + ++ + R V D Y FN E+ L ++I + T
Sbjct: 275 KEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY 334
Query: 333 EEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGK 391
+ S+ G Y + K R+N V+ G+GSDE+ GY YF E + + +
Sbjct: 335 DITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESER 392
Query: 392 LLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIW 451
LLRE LY+ V R LR F++
Sbjct: 393 LLRE------------------LYLFDVLRADRTTAAHGLELRVPFLDH----------- 423
Query: 452 KIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSA 511
+ Y SLP +R KN + + R F D L+ + P+ S
Sbjct: 424 ---------RFSSYYLSLPPEMRI-PKNGIEKHL-LRETFEDSNLIPKEILWRPKEAFSD 472
Query: 512 GITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYW 571
GIT V + IL + + ++ D + N QKF + K + +
Sbjct: 473 GITSV--KNSWFKILQEYVEHQVD---------DAMMANAAQKFPF-----NTPKTKEGY 516
Query: 572 DYRKVLRMFEEHVSGKKNWSQELW 595
YR+V FE H G+ +W W
Sbjct: 517 YYRQV---FERHYPGRADWLSHYW 537
>gb|AAC72836.1| (AF095452) asparagine synthetase [Arabidopsis thaliana]
Length = 578
Score = 130 bits (324), Expect = 4e-29
Identities = 117/392 (29%), Positives = 180/392 (45%), Gaps = 54/392 (13%)
Query: 1 MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI S + ++ +RHRGPD G++ ++ L H RLAI+D + G
Sbjct: 1 MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPT-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K + + NGEIYN ++R+ L K + F + +D EVI Y E G + V+
Sbjct: 60 DQPL-YNED-KTIAVTVNGEIYNHKDLRENL--KSHQFRTGSDCEVIAHLYEEHGEEFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM+AFV+ D +RD GI PLY + DG ++ F+SE+K +
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
++D E PG+ ++ W +
Sbjct: 165 ---------------SDDC--EQFMCFPPGH--IYSSKQGGLRRWYNPPWFSEVVPSTPY 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEI-----KVFS 286
DP ++ F +AV RL+ DVP+G LSGGLDSS + A+RHL K+ +
Sbjct: 206 -DPLVVRNTFEKAVIKRLMTDVPLGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHT 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
K + K R V D + F V+D + D I+ + E + +I
Sbjct: 265 FCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI-DAIEEVIYHVETYDVTTIRASTP 323
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEI GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355
>pir||T08846 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) 2 -
soybean >gi|1778370|gb|AAC49613.1| (U77678) asparagine
synthetase 2 [Glycine max]
Length = 581
Score = 129 bits (320), Expect = 1e-28
Identities = 120/395 (30%), Positives = 181/395 (45%), Gaps = 60/395 (15%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCFLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K V + NGEIYN E+RK+L + F + +D +VI Y E G D V+
Sbjct: 60 DQPLFNED--KSVIVTVNGEIYNHEELRKQLPN--HNFRTGSDCDVIAHLYEEHGEDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
D E + PG+ ++ R F W Y+ E I
Sbjct: 166 DDC------------------EHFECFPPGH--LYSSKERGFRRW--YNPPWFSEAIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
DP + F +AV RL+ DVP G LSGGLDSS + L + + K
Sbjct: 204 P--YDPLVLRHAFEQAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLANTKAAEQWGSK 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
+ S + + K + V D + TF V+D + I D+I E + +I
Sbjct: 262 LHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGIDSIEDVI-YHIETYDVTTIRA 320
Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K ++ G+GSDEI GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355
>pir||D82846 asparagine synthase B XF0118 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9104900|gb|AAF82931.1|AE003865_8
(AE003865) asparagine synthase B [Xylella fastidiosa]
Length = 563
Score = 129 bits (320), Expect = 1e-28
Identities = 107/368 (29%), Positives = 171/368 (46%), Gaps = 49/368 (13%)
Query: 23 RHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIR 82
RHRGPD GVYVD L H RLAI+D P G +DG + + NGEIYN ++
Sbjct: 31 RHRGPDWSGVYVDVGAILVHERLAIVD--PAGGAQPLLSEDG-TLALAVNGEIYNHAVLK 87
Query: 83 KELEEKGYTFTSNTDTEVILAAYL-EWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGI 141
L++ Y F +++D EVI A Y E + R NG++AF ++DK+ ++RD G+
Sbjct: 88 GTLQQP-YVFQTHSDCEVINALYREETPTSFLNRLNGIFAFALWDKTTGRGLIARDPMGV 146
Query: 142 KPLYYYYD-GKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLM 200
PLY+ +D + +SE+K +++H P+ A +Y+ T ++E
Sbjct: 147 VPLYWGHDQDGRLRVASEMKALIEH----CPDVAQFPPGHWYDTTTGTLVKYYE------ 196
Query: 201 PGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCL 260
+WK Y E ++ Q ++ F RAV +L+ DVP G L
Sbjct: 197 --------------RSWKNYSAVEGVQ-----VSLQELREAFERAVHRQLMTDVPYGVLL 237
Query: 261 SGGLDSSSIVCA----MRHLLPDGE------IKVFSLVFPGKEIDESKYQRIVVDECRVS 310
SGGLDSS + RH + + ++ S K+ + + +
Sbjct: 238 SGGLDSSLVAAVAARYARHRIETNDQSEAWWPRLHSFAIGLKDSPDLSAANVAAEALNTV 297
Query: 311 WYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLLDGQGSDE 367
+ + +++ L D++ + E + +I + LAR G+K++L G+GSDE
Sbjct: 298 HHGFEYTLQEGL-DVLPEVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDE 356
Query: 368 ILAGYHYF 375
I GY YF
Sbjct: 357 IFGGYLYF 364
>gb|AAB48058.1| (L40327) asparagine synthetase [Medicago sativa]
Length = 586
Score = 129 bits (320), Expect = 1e-28
Identities = 112/391 (28%), Positives = 179/391 (45%), Gaps = 51/391 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN ++RK+L + F + D +VI Y E+G + V+
Sbjct: 60 DQPLFNED--KSIIVTVNGEIYNHEDLRKQLPN--HKFRTQCDCDVIAHLYEEYGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ +SE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ R+F W
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKDREFRRWYNPPWFNEAVIPTTP 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
DP ++ F +AV RL+ DVP G LSGGLDSS + L + K+ S
Sbjct: 206 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGAKLHS 265
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
K + K R V D + F ++D + I D+I T+ +T+ +
Sbjct: 266 FCVGLKGAPDLKAGREVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325
Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ + + + +G+K ++ G+GSDEI GY YF
Sbjct: 326 FLMSRKIKSSGVKWVISGEGSDEIFGGYLYF 356
>gb|AAB81011.1| (U89923) asparagine synthetase [Medicago sativa]
Length = 586
Score = 129 bits (320), Expect = 1e-28
Identities = 112/391 (28%), Positives = 179/391 (45%), Gaps = 51/391 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN ++RK+L + F + D +VI Y E+G + V+
Sbjct: 60 DQPLFNED--KSIIVTVNGEIYNHEDLRKQLPN--HKFRTQCDCDVIAHLYEEYGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ +SE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ R+F W
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKDREFRRWYNPPWFNEAVIPTTP 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
DP ++ F +AV RL+ DVP G LSGGLDSS + L + K+ S
Sbjct: 206 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGAKLHS 265
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
K + K R V D + F ++D + I D+I T+ +T+ +
Sbjct: 266 FCVGLKGAPDLKAGREVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325
Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ + + + +G+K ++ G+GSDEI GY YF
Sbjct: 326 FLMSRKIKSSGVKWVISGEGSDEIFGGYLYF 356
>pir||T50812 asparagine synthetase ASN3 - Arabidopsis thaliana
>gi|8953405|emb|CAB96680.1| (AL360334) asparagine
synthetase ASN3 [Arabidopsis thaliana]
Length = 578
Score = 128 bits (319), Expect = 2e-28
Identities = 117/392 (29%), Positives = 178/392 (44%), Gaps = 54/392 (13%)
Query: 1 MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI S + ++ +RHRGPD G++ ++ L H RLAI+D + G
Sbjct: 1 MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPT-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K + + NGEIYN +R+ L K + F + +D EVI Y E G + V+
Sbjct: 60 DQPL-YNED-KTIAVTVNGEIYNHKALRENL--KSHQFRTGSDCEVIAHLYEEHGEEFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM+AFV+ D +RD GI PLY + DG ++ F+SE+K +
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
++D E PG+ ++ W +
Sbjct: 165 ---------------SDDC--EQFMCFPPGH--IYSSKQGGLRRWYNPPWFSEVVPSTPY 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEI-----KVFS 286
DP ++ F +AV RL+ DVP G LSGGLDSS + A+RHL K+ +
Sbjct: 206 -DPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHT 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
K + K R V D + F V+D + D I+ + E + +I
Sbjct: 265 FCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI-DAIEEVIYHVETYDVTTIRASTP 323
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEI GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355
>sp|P49094|ASNS_MAIZE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|7438077|pir||T02978
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- maize >gi|984262|emb|CAA58052.1| (X82849) asparragine
synthetase [Zea mays]
Length = 586
Score = 128 bits (319), Expect = 2e-28
Identities = 117/387 (30%), Positives = 177/387 (45%), Gaps = 54/387 (13%)
Query: 3 GINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEK 62
G+ S + ++ +RHRGPD G++ ++ L H RLAIID + G QP+ Y +
Sbjct: 9 GVVEVSLAKRSRIIELSRRLRHRGPDWSGLHCHEDCYLAHQRLAIIDPT-SGDQPL-YNE 66
Query: 63 DGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAF 122
D K V + NGEIYN E++ +L K + F + +D EVI Y E+G + V+ +GM++F
Sbjct: 67 D-KTVVVTVNGEIYNHEELKAKL--KTHEFQTGSDCEVIAHLYEEYGEEFVDMLDGMFSF 123
Query: 123 VIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGI---LQHNIPRKPNDAVIF 177
V+ D +RD GI PLY + DG ++ FSSE+K + + I P
Sbjct: 124 VLLDTRDKSFIAARDAIGICPLYMGWGLDG-SVWFSSEMKALSDDCERFITFPPG----- 177
Query: 178 DFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQG 237
H + G++R YN + T + LRE EK
Sbjct: 178 ---------HLYSSKTGGLRR---WYNPPWFSETVPSTPYNALFLREMFEK--------- 216
Query: 238 FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFSLVFPG 291
AV RL+ DVP G LSGGLDSS + L + ++ K+ +
Sbjct: 217 -------AVIKRLMTDVPFGVLLSGGLDSSLVASVASRHLNETKVDRQWGNKLHTFCIGL 269
Query: 292 KEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVM 348
K + K R V D + F V+ D L ++I I T + S + +
Sbjct: 270 KGSPDLKAAREVADYLSTVHHEFHFTVQEGIDALEEVIYHIETYDVTTIRAST-PMFLMS 328
Query: 349 KLARENGMKVLLDGQGSDEILAGYHYF 375
+ + G+K+++ G+GSDEI GY YF
Sbjct: 329 RKIKSLGVKMVISGEGSDEIFGGYLYF 355
>sp|P49078|ASNS_ARATH ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|7438081|pir||T12989
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- Arabidopsis thaliana >gi|507946|gb|AAA74359.1|
(L29083) glutamine-dependent asparagine synthetase
[Arabidopsis thaliana] >gi|5541701|emb|CAB51206.1|
(AL096860) glutamine-dependent asparagine synthetase
[Arabidopsis thaliana]
Length = 584
Score = 128 bits (319), Expect = 2e-28
Identities = 119/392 (30%), Positives = 179/392 (45%), Gaps = 54/392 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ +RHRGPD G+Y + + L H RLA+ID + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+RK L K + F + +D EVI Y E+G D V+
Sbjct: 60 DQPLFNED--KTIVVTVNGEIYNHEELRKRL--KNHKFRTGSDCEVIAHLYEEYGVDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ + F+ W Y
Sbjct: 166 DDC------------------EHFETFPPGH--FYSSKLGGFKQW-YNPPWFNESVPSTP 204
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VFS 286
+P + F AV RL+ DVP G LSGGLDSS + RHL K + S
Sbjct: 205 YEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKQWGPQLHS 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
+ + K + V + + F+V+D + I D+I E + +I
Sbjct: 265 FCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGIDAIEDVI-YHVETYDVTTIRASTP 323
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+G+DEI GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVLSGEGADEIFGGYLYF 355
>gb|AAF74755.1| (AF263432) asparagine synthetase [Helianthus annuus]
Length = 589
Score = 128 bits (318), Expect = 2e-28
Identities = 124/393 (31%), Positives = 183/393 (46%), Gaps = 56/393 (14%)
Query: 1 MCGINGF-SWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G+Y + L H RLA+ID + G
Sbjct: 1 MCGILALLGCSDDSQAKRFRVLELSRRLKHRGPDWSGLYQHGDNYLSHQRLAVIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D + V + NGEIYN ME+R++L G+ F + +D +VI Y E G + V+
Sbjct: 60 DQPL-YNED-ETVVVTVNGEIYNHMELREKL--MGHKFKTGSDCDVIAHLYEEHGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM++FV+ N ++RD GI LY + DG +++ SS +KG+ N
Sbjct: 116 MLDGMFSFVLLGTRDNSYLVARDAIGITSLYIGWGLDG-SVLISSGLKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D +H E F G ++ R F W Y+L E
Sbjct: 166 DD----------CEHFE-VFLAG---------HLYSSKIRGFRRW--YNLPWFSESIPST 203
Query: 233 S-DPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VF 285
DP + F AV RL+ DVP G LSGGLDSS + RHL K +
Sbjct: 204 PYDPLVLRCAFENAVIKRLMTDVPFGVLLSGGLDSSLVASVTARHLAGSKAAKQWGAQLH 263
Query: 286 SLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQY 345
S + + K R V D + F V+D + I D+I E + +I
Sbjct: 264 SFCVGLEGSPDLKAAREVADYLGTLHHEFHFTVQDGIDAIEDVI-YHIETYDVTTIRAST 322
Query: 346 RVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K+++ G+GSDEI GY YF
Sbjct: 323 PMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355
>dbj|BAA96251.1| (AB035247) asparagine synthetase [Astragalus sinicus]
Length = 584
Score = 128 bits (318), Expect = 2e-28
Identities = 118/392 (30%), Positives = 178/392 (45%), Gaps = 54/392 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCADASRAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K V + NGEIYN ++RK+L +TF + +D +VI Y E+G D V+
Sbjct: 60 DQPLFNED--KSVIVTVNGEIYNHEDLRKQLPN--HTFRTGSDCDVIAHLYEEYGEDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSYIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ F W Y L
Sbjct: 166 DDC------------------EHFECFPPGH--LYSSKNGGFRRW-YNPLWYSEAIPSAP 204
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFS 286
DP + F +AV RL+ DVP G LSGGLDSS + L + K+ S
Sbjct: 205 YDPIVLRQAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHS 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
+ + K + V D + TF V+D + I D+I E + +I
Sbjct: 265 FCVGLEGSPDLKAGKEVADYLGTVHHEFTFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 323
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K ++ G+GSDEI GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355
>gb|AAC16325.1| (AF061740) asparagine synthetase [Elaeagnus umbellata]
Length = 585
Score = 128 bits (318), Expect = 2e-28
Identities = 122/392 (31%), Positives = 180/392 (45%), Gaps = 54/392 (13%)
Query: 1 MCGINGF-SWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAIID + G
Sbjct: 1 MCGILALLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLFQFGDCYLAHQRLAIIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K V + NGEIYN E+RK+L + F + +D +VI Y E+G D V+
Sbjct: 60 DQPLFNED--KTVVVTVNGEIYNHEELRKKLSH--HKFRTGSDCDVIAHLYEEYGEDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G++AFV+ D N ++RD G+ LY + DG +I SSE+KG+ N
Sbjct: 116 MLDGIFAFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SIWISSELKGL--------NN 166
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D F+ H + G++R YN V+ T +
Sbjct: 167 DCEHFETFPPG---HLYSSKAGGMRR---WYNPVWFSETIPSAPY--------------- 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHL-----LPDGEIKVFS 286
DP + F AV RL+ DVP G LSGGLDSS + RHL K+ S
Sbjct: 206 -DPLALRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLSGTKAAKQWGTKLHS 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
+ + K + V + F V+D + I D+I E + +I
Sbjct: 265 FCVGLEGSPDLKAAKEVAAYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 323
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K+++ G+GSDEI GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVMSGEGSDEIFGGYLYF 355
>sp|P49093|ASN2_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE 2)
>gi|2147132|pir||S57932 asparagine synthase
(glutamine-hydrolysing) (EC 6.3.5.4) - Lotus japonicus
>gi|2147134|pir||S69183 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 2 - Lotus japonicus
>gi|897773|emb|CAA61590.1| (X89410) asparagine synthase
(glutamine-hydrolysing) [Lotus japonicus]
Length = 586
Score = 128 bits (318), Expect = 2e-28
Identities = 116/394 (29%), Positives = 182/394 (45%), Gaps = 58/394 (14%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDNFLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E + + + NGEI+N E+RK+L + F + D +VI Y E G + V+
Sbjct: 60 DQPLFNED--QSIIVTVNGEIFNHEELRKQLPN--HKFRTGCDCDVIAHLYEEHGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY Y DG ++ +SE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDG-SVWIASELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
D D ++ L PG+ ++ ++F W Y+ E I
Sbjct: 166 D----DCEHFELFP--------------PGH--LYSSKEKEFRRW--YNPPWFSEAIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
DP + F +A+ RL+ DVP G LSGGLDSS + L D + K
Sbjct: 204 P--YDPLALRQAFEKAIIKRLMTDVPFGVLLSGGLDSSLVASVTARYLADTKAAKQWGSK 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
+ S + + K R V D + + ++D + I D+I E T G
Sbjct: 262 LHSFCVGLEGAPDLKAAREVADYIGTVHHEFQYTIQDGIDAIEDVIYHVETYDVTTIRAG 321
Query: 344 --QYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ + + + G+K++L G+GSDEI AGY YF
Sbjct: 322 TPMFLMSRKIKSLGVKMVLSGEGSDEIFAGYLYF 355
>sp|Q43011|ASNS_ORYSA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|7438076|pir||T03602 probable
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- rice >gi|1432054|gb|AAB03991.1| (U55873) asparagine
synthetase [Oryza sativa] >gi|1902992|dbj|BAA18951.1|
(D83378) asparagine synthetase [Oryza sativa]
Length = 591
Score = 127 bits (316), Expect = 4e-28
Identities = 117/395 (29%), Positives = 182/395 (45%), Gaps = 60/395 (15%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D L KR ++ +RHRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGVADVSLAKRSRIIELSRRLRHRGPDWSGIHCYQDCYLAHQRLAIVDPT-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K V + NGEIYN E++ L K + F + +D EVI Y E+G + V+
Sbjct: 60 DQPL-YNED-KSVVVTVNGEIYNHEELKANL--KSHKFQTASDCEVIAHLYEEYGEEFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGI---LQHNIPR 169
+GM+AFV+ D +RD GI PLY + DG ++ FSSE+K + + I
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGICPLYMGWGLDG-SVWFSSEMKALSDDCERFISF 174
Query: 170 KPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKR 229
P H + G++R YN + + + LR+ EK
Sbjct: 175 PPG--------------HLYSSKTGGLRR---WYNPPWFSESIPSTPYNPLLLRQSFEK- 216
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
A+ RL+ DVP G LSGGLDSS + + L + ++ K
Sbjct: 217 ---------------AIIKRLMTDVPFGVLLSGGLDSSLVASVVSRHLAEAKVAAQWGNK 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
+ + K + + + V D + F V++ + D ++ + E + +I
Sbjct: 262 LHTFCIGLKGSPDLRAAKEVADYLGTVHHELHFTVQEGI-DALEEVIYHVETYDVTTIRA 320
Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEI GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355
>sp|P31752|ASNS_ASPOF ASPARAGINE SYNTHASE [GLUTAMINE-HYDROLYZING] (AS)
>gi|100980|pir||S25165 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) - garden asparagus
>gi|16076|emb|CAA48141.1| (X67958) asparagine synthase
(glutamine-hydrolysing) [Asparagus officinalis]
Length = 590
Score = 127 bits (315), Expect = 5e-28
Identities = 125/397 (31%), Positives = 184/397 (45%), Gaps = 64/397 (16%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G+ + L H RLAIID + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K + + NGEIYN E+R+ L + Y + +D EVI Y E G D V+
Sbjct: 60 DQPL-YNED-KSIVVTVNGEIYNHEELRRRLPDHKYR--TGSDCEVIAHLYEEHGEDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM++FV+ D N +RD GI PLY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDG-SVWLSSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
D +H E + P N ++ + F W ++Y+ E I
Sbjct: 166 DD----------CEHFE---------VFPPGN-LYSSRSGSFRRWYNPQWYN--ETIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GE 281
DP + F AV RL+ DVP G LSGGLDSS + RHL +
Sbjct: 204 P--YDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQ 261
Query: 282 IKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSI 341
+ F + G + K + V + + F V+D + I D+I E + +I
Sbjct: 262 LHSFCVGLEGS--PDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-FHIETYDVTTI 318
Query: 342 YGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ +AR+ G+K+++ G+GSDEI GY YF
Sbjct: 319 RASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYF 355
>emb|CAA67889.1| (X99552) asparagine synthetase [Asparagus officinalis]
Length = 590
Score = 127 bits (315), Expect = 5e-28
Identities = 125/397 (31%), Positives = 184/397 (45%), Gaps = 64/397 (16%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G+ + L H RLAIID + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K + + NGEIYN E+R+ L + Y + +D EVI Y E G D V+
Sbjct: 60 DQPL-YNED-KSIVVTVNGEIYNHEELRRRLPDHKYR--TGSDCEVIAHLYEEHGEDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM++FV+ D N +RD GI PLY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDG-SVWLSSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
D +H E + P N ++ + F W ++Y+ E I
Sbjct: 166 DD----------CEHFE---------VFPPGN-LYSSRSGSFRRWYNPQWYN--ETIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GE 281
DP + F AV RL+ DVP G LSGGLDSS + RHL +
Sbjct: 204 P--YDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQ 261
Query: 282 IKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSI 341
+ F + G + K + V + + F V+D + I D+I E + +I
Sbjct: 262 LHSFCVGLEGS--PDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-FHIETYDVTTI 318
Query: 342 YGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ +AR+ G+K+++ G+GSDEI GY YF
Sbjct: 319 RASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYF 355
>sp|P19251|ASN1_PEA ASPARAGINE SYNTHETASE, NODULE [GLUTAMINE-HYDROLYZING]
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE)
>gi|68661|pir||AJPMN1 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 1 - garden pea
>gi|20650|emb|CAA36429.1| (X52179) asparagine synthase
(glutamine-hydrolysing) [Pisum sativum]
Length = 586
Score = 127 bits (315), Expect = 5e-28
Identities = 113/391 (28%), Positives = 179/391 (44%), Gaps = 51/391 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+RK+L + F + D +VI Y E G + V+
Sbjct: 60 DQPLFNED--KSIIVTVNGEIYNHEELRKQLPN--HKFFTQCDCDVIAHLYEEHGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ +SE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ R+F W
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKEREFRRWYNPPWFNEAIIPSTP 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIKVFSLVFPG 291
DP ++ F +AV RL+ DVP G LSGGLDSS + R+L K + P
Sbjct: 206 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLAGTKAAKQWGAKLPS 265
Query: 292 -----KEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
K + K + V D + F ++D + I D+I T+ +T+ +
Sbjct: 266 FCVGLKGAPDLKAGKEVADFLGTVHHEFEFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325
Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ + + + +G+K ++ G+GSDEI GY YF
Sbjct: 326 FLMSRKIKSSGVKWVISGEGSDEIFGGYLYF 356
>sp|P49092|ASN1_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 1
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE 1)
>gi|2147131|pir||S57931 asparagine synthase
(glutamine-hydrolysing) (EC 6.3.5.4) - Lotus japonicus
>gi|2147133|pir||S69182 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 1 - Lotus japonicus
>gi|897771|emb|CAA61589.1| (X89409) asparagine synthase
(glutamine-hydrolysing) [Lotus japonicus]
Length = 586
Score = 126 bits (314), Expect = 6e-28
Identities = 113/392 (28%), Positives = 177/392 (44%), Gaps = 54/392 (13%)
Query: 1 MCGI------NGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI + F+ V ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+RK+L + F + +D +VI Y E G + ++
Sbjct: 60 DQPLFNED--KSIIVTVNGEIYNHEELRKQLPN--HQFRTGSDCDVIAHLYEEHGENFMD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ R F W Y
Sbjct: 166 DDC------------------EHFEVFPPGH--LYSSRERAFRRW-YNPTWFSESIPSAP 204
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFS 286
DP + F +AV RL+ DVP G LSGGLDSS + L + K+ S
Sbjct: 205 YDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHS 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
+ + K + V D + TF V+D + D I+ + E + +I
Sbjct: 265 FCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGI-DAIEEVIYHVETYDVTTIRASTP 323
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K ++ G+GSDEI GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355
>pir||JQ2245 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - garden
asparagus
Length = 590
Score = 125 bits (312), Expect = 1e-27
Identities = 151/536 (28%), Positives = 224/536 (41%), Gaps = 121/536 (22%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G+ + L H RLAIID + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K + + NGEIYN E+R+ L + Y + +D EVI Y E G D V+
Sbjct: 60 DQPL-YNED-KSIVVTVNGEIYNHEELRRRLPDHKYR--TGSDCEVIAHLYEEHGEDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+GM++FV+ D N +RD GI PLY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDG-SVWLSSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
D +H E + P N ++ + F W ++Y+ E I
Sbjct: 166 DD----------CEHFE---------VFPPGN-LYSSRSGSFRRWYNPQWYN--ETIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLV 288
DP + F AV RL+ DVP G LSGGLDSS + RHL
Sbjct: 204 P--YDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHL------------ 249
Query: 289 FPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVM 348
G + E W + L DL +E +
Sbjct: 250 -AGSKAAE-------------QWGTQLHSFCVGLEGSPDLKAAKE------------LMA 283
Query: 349 KLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVP 408
+ + G+K+++ G+GSDEI GY YF E K+ K +H
Sbjct: 284 RKIKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHHETCRKI-------KALHQYDCL 336
Query: 409 LKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYS 468
N+ Y+ V F + ++ I+ +++I+ I+T + + A T
Sbjct: 337 RANVAEYLGTV-------HHEFHFTVQDGIDAI-----EDVIFHIETYDVTTIRASTPMF 384
Query: 469 LPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKV----SAGITKVLLRK 520
K W +E+RVPF D E ++ +S+ PESK+ I K +LRK
Sbjct: 385 ---------KATSAWGLEARVPFLDKEFMDVAMSIDPESKMIKPDLGRIEKWVLRK 431
>sp|Q61024|ASNS_MOUSE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|1147605|gb|AAA85125.1|
(U38940) asparagine synthetase [Mus musculus]
Length = 561
Score = 125 bits (312), Expect = 1e-27
Identities = 161/636 (25%), Positives = 259/636 (40%), Gaps = 140/636 (22%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI D+ L + A++ HRGPD E V N G RLA++D G
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
QP++ K +W+ YNGEIYN +++ E + + +N D E+IL Y + G + +
Sbjct: 60 QPIRVRKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEKTIC 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGI--LQHN----- 166
+G++AF++ D + +FL RD +G++PL+ + SE KG+ L+H+
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFL 175
Query: 167 ------------IPRKPNDAV--IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR 212
+ KPN V + Y++ D ++ +++L PG FDL T
Sbjct: 176 KVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDSVEKLFPG----FDLETV 231
Query: 213 KFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA 272
K + LF A+K RL+ D +G LSGGLDSS + +
Sbjct: 232 K----------------------NNLRILFDNAIKKRLMTDRRIGCLLSGGLDSSLVAAS 269
Query: 273 MRHLLPDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIID 327
+ L + +++ + + ++ + R V + + FN E+ L ++I
Sbjct: 270 LLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIF 329
Query: 328 LIRTQEEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRK 386
+ T + S+ G Y + K R+N V+ G+GSDE+ GY YF E +
Sbjct: 330 SLETYDITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE 388
Query: 387 FKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPS 446
+ +LL+E+ + + + + R P+L F + P
Sbjct: 389 -ESERLLKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPP 434
Query: 447 KELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPE 506
I K E LL ET FED N + I R P+
Sbjct: 435 DMRI--PKNGIEKHLLRET---------FEDCNLLPKEILWR----------------PK 467
Query: 507 SKVSAGITKV------LLRKGLKGILPDEIRNRAS-KVGFATPDKDILKTNEGQKFALAV 559
S GIT V +L+ ++ + DE+ + AS K F TP KT EG
Sbjct: 468 EAFSDGITSVKNSWFKILQDYVEHQVDDEMMSAASQKFPFNTP-----KTKEG------- 515
Query: 560 IDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELW 595
+ YR++ FE H G+ +W W
Sbjct: 516 -----------YFYRQI---FERHYPGRADWLTHYW 537
>sp|O24338|ASNS_SANAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|2245622|gb|AAB71532.1|
(AF005724) asparagine synthetase [Sandersonia
aurantiaca]
Length = 525
Score = 125 bits (311), Expect = 1e-27
Identities = 119/393 (30%), Positives = 179/393 (45%), Gaps = 56/393 (14%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ ++HRGPD G+ + L H RLAIID + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLDHHGDCYLAHQRLAIIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ Y +D K + + NGEIYN E+RK L G+TF + +D EVI Y E G +
Sbjct: 60 DQPL-YNED-KTIIVTVNGEIYNHEELRKGLP--GHTFRTGSDCEVIAHLYEEHGESFIH 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N +RD G+ PLY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGIFSFVLLDSRNNSFVAARDAIGVTPLYIGWGLDG-SVWISSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E K PG+ ++ F+ W Y+ E +
Sbjct: 166 DDC------------------EHFKFFPPGH--LYSSKEGSFKRW--YNPPWFSEVIPSV 203
Query: 233 S-DPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVF 285
DP + F AV RL+ DVP G LSGGLDSS + L + ++
Sbjct: 204 PFDPLALRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLEGTKAAELWGTQLH 263
Query: 286 SLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQY 345
S + + K + V + + F V+D + I D+I E + +I
Sbjct: 264 SFCVGLEGSPDLKAAKEVANFLGTIHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRAST 322
Query: 346 RVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K+++ G+GSDEI GY YF
Sbjct: 323 PMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355
>prf||2207183A Asn synthetase [Rattus norvegicus]
Length = 561
Score = 125 bits (310), Expect = 2e-27
Identities = 156/623 (25%), Positives = 254/623 (40%), Gaps = 114/623 (18%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI D+ L + A++ HRGPD E V N G RLA++D G
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
QP++ K +W+ YNGEIYN +++ E + + +N D E+IL Y + G + +
Sbjct: 60 QPIRVRKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEKTIC 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
+G++AF++ D + +FL RD +G++PL+ + SE KG++
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKALTEDGFLAVCSEAKGLVS--------- 166
Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
L H+ F + ++ +PG+ V DL E KY
Sbjct: 167 -----------LKHSTTPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHA 214
Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
YD E++ EI + + LF A+K RL+ D +G LSGGLDSS + ++ L
Sbjct: 215 IYDSVEKLFPGFEIETVKNNLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQL 274
Query: 278 PDGEIKVFSLVFP--GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQE-- 333
+ ++ F ++ + R V + + FN E+ + + ++I + E
Sbjct: 275 KEAQVPYALQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFSLETY 334
Query: 334 EPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKL 392
+ + + G Y + K R+N V+ G+GSDE+ GY YF E + + +L
Sbjct: 335 DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESERL 393
Query: 393 LREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWK 452
L+E+ + + + + R P+L F + P + I K
Sbjct: 394 LKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPPEMRIPK 440
Query: 453 IKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAG 512
E LL ET FED N + I R P+ S G
Sbjct: 441 DGI--EKHLLRET---------FEDSNLLPKEILWR----------------PKEAFSDG 473
Query: 513 ITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWD 572
IT V + IL D + ++ D + + QKF + K Y+
Sbjct: 474 ITSV--KNSWFKILQDFVEHQVD---------DAMMSEASQKFPFNTPQT----KEGYY- 517
Query: 573 YRKVLRMFEEHVSGKKNWSQELW 595
YR++ FE H G+ +W W
Sbjct: 518 YRQI---FEHHYPGRADWLTHYW 537
>pir||JW0071 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - soybean
>gi|1305549|gb|AAC09952.1| (U55874) asparagine
synthetase [Glycine max]
Length = 579
Score = 124 bits (308), Expect = 3e-27
Identities = 117/395 (29%), Positives = 175/395 (43%), Gaps = 60/395 (15%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D KR ++ ++HRGPD G++ + L H RLAI+D G
Sbjct: 1 MCGILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGDNYLAHQRLAIVD-PASG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K V + NGEIYN E+RK+L +TF + +D +VI Y E G + V+
Sbjct: 60 DQPLFNE--DKTVVVTVNGEIYNHEELRKQL--PNHTFRTGSDCDVIAHLYEEHGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++F + D N ++RD G+ LY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGIFSFALLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
D E + PG+ ++ R F W Y+ E I
Sbjct: 166 DDC------------------EHFESFPPGH--LYSSKERAFRRW--YNPPWFSEAIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IK 283
DP + F +AV RL+ DVP G LSGGLDSS + L K
Sbjct: 204 P--YDPLALRHAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTNAAKQWGTK 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLS 340
+ S + + K + V D + + V+ D + D+I I T + S
Sbjct: 262 LHSFCVGLEGAPDLKAAKEVADYIGTVHHEFHYTVQDGIDAIEDVIYHIETYDVTTIRAS 321
Query: 341 IYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
I + + + + G+K ++ G+GSDEI GY YF
Sbjct: 322 I-PMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355
>pir||T39308 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) SPBC119.10
[similarity] - fission yeast (Schizosaccharomyces pombe)
>gi|2959371|emb|CAA17925.1| (AL022117) asparagine
synthetase [Schizosaccharomyces pombe]
Length = 557
Score = 124 bits (308), Expect = 3e-27
Identities = 122/407 (29%), Positives = 185/407 (44%), Gaps = 71/407 (17%)
Query: 1 MCGINGFSWGDEEL------VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI E++ ++ +RHRGPD G + + L H RLAI+ + G
Sbjct: 1 MCGILAVHHVAEDIEAFKPKALHLSKQLRHRGPDWSGKAIRNQTILCHERLAIVGVE-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ + DGK V V NGEIYN +++R+ L+ Y F + +D EVIL Y E G C
Sbjct: 60 AQPLVSD-DGKLVLTV-NGEIYNHLKLRENLKGN-YKFKTYSDCEVILYLYREHGPACAN 116
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKN---IIFSSEIKGILQHNIPRKP 171
+GM+++V+YD+ K+ + +RD GI LY + + F+SE+K + H + K
Sbjct: 117 MLDGMFSWVLYDQDKDKVVAARDPIGITTLYQGFSSDSPDTAYFASELKAL--HPVCDK- 173
Query: 172 NDAVIFDF---LYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEK 228
I F YY+ T+ T ++ P + +D N Y LRE +E
Sbjct: 174 ----IIAFPPGHYYD--SETKQT----VRYFKPSW---WDENKIPSNPVDYKLLRETLE- 219
Query: 229 RKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC----------------- 271
+V+ RL+A+VP G LSGGLDSS I
Sbjct: 220 ---------------ASVRKRLMAEVPYGVLLSGGLDSSLIASIAARETEKLANSTSQSE 264
Query: 272 AMRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDL 328
R + ++ F++ PG + R V D + TF ++ D L D+I
Sbjct: 265 EARTITAWPKLHSFAIGLPGS--PDLLAARKVADFLHTFHHEHTFTIDEGLDALRDVIYH 322
Query: 329 IRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ T + S Y + + + G+K++L G+GSDEI GY YF
Sbjct: 323 LETYDVTTIRAST-PMYLLSRKIKAQGVKMVLSGEGSDEIFGGYLYF 368
>sp|P19891|ASNS_CRIGR ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|68657|pir||AJHYNC asparagine
synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - Chinese
hamster >gi|191064|gb|AAA36977.1| (M27838) asparagine
synthetase [Cricetulus longicaudatus]
Length = 561
Score = 123 bits (306), Expect = 5e-27
Identities = 155/624 (24%), Positives = 256/624 (40%), Gaps = 116/624 (18%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI D+ L + A++ HRGPD E V N G RLA++D G
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
QP++ +K +W+ YNGEIYN +++ E + + +N D E+IL Y + G + +
Sbjct: 60 QPIRVKKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEQTIC 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
+G++AF++ D + +FL RD +G++PL+ + SE KG++
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVS--------- 166
Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKYYDLRER---- 225
L H+ F + ++ +PG+ V DL E KY+ R+
Sbjct: 167 -----------LKHSTTPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHA 214
Query: 226 ----IEKRKEISDPQGFKD----LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
+EK + + + K LF AV+ RL+ D +G LSGGLDSS + ++ L
Sbjct: 215 LYDSVEKLFQGFELETVKSNLRILFDSAVRKRLMTDRRIGCLLSGGLDSSLVAASLLKQL 274
Query: 278 PDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIIDLIRTQ 332
+ +++ + + ++ + R V + + FN E+ L ++I + T
Sbjct: 275 KEAQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIFSLETY 334
Query: 333 EEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGK 391
+ S+ G Y + K R+N V+ G+GSDE+ GY YF E + + +
Sbjct: 335 DITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESER 392
Query: 392 LLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIW 451
LL+E+ + + + + R P+L F + P + I
Sbjct: 393 LLKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPPEMRI- 438
Query: 452 KIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSA 511
K E LL ET FED N + I R P+ S
Sbjct: 439 -PKNGIEKHLLRET---------FEDSNLLPKEILWR----------------PKEAFSD 472
Query: 512 GITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYW 571
GIT V + IL D + ++ D + QKF + K + +
Sbjct: 473 GITSV--KNSWFKILQDYVEHQVD---------DEMMATAAQKFPF-----NTPKTKEGY 516
Query: 572 DYRKVLRMFEEHVSGKKNWSQELW 595
YR++ FE H G+ +W W
Sbjct: 517 YYRQI---FERHYPGRADWLTHYW 537
>sp|P49088|ASNS_RAT ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|1083600|pir||S53447
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
[validated] - rat >gi|460631|gb|AAA77671.1| (U07201)
asparagine synthetase [Rattus norvegicus]
>gi|460633|gb|AAA77672.1| (U07202) asparagine synthetase
[Rattus norvegicus]
Length = 561
Score = 123 bits (305), Expect = 7e-27
Identities = 156/623 (25%), Positives = 252/623 (40%), Gaps = 114/623 (18%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI D+ L + A++ HRGPD E V N G RLA++D G
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
QP++ K +W+ YNGEIYN +++ E + + +N D E+IL Y + G + +
Sbjct: 60 QPIRVRKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEKTIC 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
+G++AF++ D + +FL RD +G++PL+ + SE KG++
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKALTEDGFLAVCSEAKGLVS--------- 166
Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
L H+ F + ++ +PG+ V DL E KY
Sbjct: 167 -----------LKHSTTPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHA 214
Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
YD E++ EI + + LF A+K RL+ D +G LSGGLDSS + ++ L
Sbjct: 215 IYDSVEKLFPGFEIETVKNNLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQL 274
Query: 278 PDGEIKVFSLVFP--GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQE-- 333
+ ++ F ++ + R V + + FN E+ + + ++I E
Sbjct: 275 KEAQVPYALQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFPLETY 334
Query: 334 EPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKL 392
+ + + G Y + K R+N V+ G+GSDE+ GY YF E + + +L
Sbjct: 335 DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESERL 393
Query: 393 LREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWK 452
L+E+ + + + + R P+L F + P + I K
Sbjct: 394 LKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPPEMRIPK 440
Query: 453 IKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAG 512
E LL ET FED N + I R P+ S G
Sbjct: 441 DGI--EKHLLRET---------FEDSNLLPKEILWR----------------PKEAFSDG 473
Query: 513 ITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWD 572
IT V + IL D + + D + + QKF + K Y+
Sbjct: 474 ITSV--KNSWFKILQDFVEYQVD---------DAMMSEASQKFPFNTPQT----KEGYY- 517
Query: 573 YRKVLRMFEEHVSGKKNWSQELW 595
YR++ FE H G+ +W W
Sbjct: 518 YRQI---FEHHYPGRADWLTHYW 537
>gb|AAB95197.1| (U84118) asparagine synthetase [Aedes aegypti]
Length = 562
Score = 123 bits (305), Expect = 7e-27
Identities = 113/383 (29%), Positives = 187/383 (48%), Gaps = 59/383 (15%)
Query: 13 ELVKRMNDAIRHRGPDDEGVYVDDNVS--LGHVRLAIIDLSPKGHQPMKYEKDGKEVWIV 70
EL R + RHRGPD G+ D++ L RL +I + G QP +DG +V +V
Sbjct: 31 ELAFRQSARQRHRGPDHTGLVADEDEGFVLVQERLCVIGVKT-GTQPF-LSQDG-QVLLV 87
Query: 71 YNGEIYNFM----EIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV-ERFNGMWAFVIY 125
NGEIYN++ EI L+ +T +D +VI+A Y ++G D + + GM+AFV+Y
Sbjct: 88 ANGEIYNYLLMAQEINDALKAGENMYTPRSDCDVIIAYYEKFGVDALMKNIRGMFAFVLY 147
Query: 126 DKSKNILFLSRDRFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFD--FLYY 182
DK + ++RD GI PLY D + N+ +SE+K +++ P + IF +YY
Sbjct: 148 DKKNGCILVARDPIGIVPLYSGKDAEGNLWIASEMKCLVE-TCP----EVAIFPPGHMYY 202
Query: 183 NLLDHTED-TFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDL 241
+ + ++FE + ++++ K + ER+ K E
Sbjct: 203 GSREKLKSVSYFES--------DWMYEIPQAKVDL-------ERLNKSLE---------- 237
Query: 242 FLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL-----PDGEIKVFSL-VFPGKEID 295
AV+ L DVP+G+ LSGGLDSS I ++ PD +K +S+ + G + +
Sbjct: 238 --DAVESHLQCDVPMGALLSGGLDSSLIASIATKIMKKRHGPDYRLKTYSVGLVRGPDFE 295
Query: 296 ESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLAR--- 352
+ R+V D F +++ L I D + E + ++ ++ L+R
Sbjct: 296 ---FARMVADYIGSDHTEVYFTIDEGLNYIRDAV-LHGETYDITTVRCTIPLLLLSRYIK 351
Query: 353 ENGMKVLLDGQGSDEILAGYHYF 375
G+K++L G+G+DE+ GY YF
Sbjct: 352 SEGIKMVLSGEGADELFGGYLYF 374
>emb|CAB57292.1| (AJ133522) asparagine synthetase (type-I) [Phaseolus vulgaris]
Length = 579
Score = 123 bits (305), Expect = 7e-27
Identities = 116/395 (29%), Positives = 177/395 (44%), Gaps = 60/395 (15%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGDCYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+RK+L +TF + +D +VI Y E G + V+
Sbjct: 60 DQPLFNED--KTIAVTVNGEIYNHEELRKQLPN--HTFRTGSDCDVIAHLYEEHGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSELKGL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
+ E + PG+ ++ R+F W Y+ E I
Sbjct: 165 -----------------NGDCEHFESFPPGH--LYSSKEREFRRW--YNPPWFSEAIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IK 283
DP + F +AV RL+ DVP G LSGGLDSS + L K
Sbjct: 204 P--YDPLALRHAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLEGTNAAKHWGTK 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLS 340
+ S + + K R V D + ++V+ D + D+I I T + S
Sbjct: 262 LHSFCVGLEGAPDLKAAREVADYIGTVHHEFHYSVQDGIDAIEDVIYHIETYDVTTIRAS 321
Query: 341 IYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
I + + + + G+K + G+GSDEI GY YF
Sbjct: 322 I-PMFLMSRKIKSLGVKWDICGEGSDEIYGGYLYF 355
>pir||AJHUN2 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) (version
2) - human >gi|339987|gb|AAA36781.1| (M15798) ts11 cell
cycle control protein [Homo sapiens]
Length = 540
Score = 122 bits (303), Expect = 1e-26
Identities = 153/602 (25%), Positives = 245/602 (40%), Gaps = 116/602 (19%)
Query: 22 IRHRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNF 78
I HRGPD E V N G RLA++D G QP++ +K +W+ YNGEIYN
Sbjct: 3 IAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGMQPIRVKKY-PYLWLCYNGEIYNH 60
Query: 79 MEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVERFNGMWAFVIYDKSKNILFLSRD 137
++++ E + + + D E+IL Y + G + + +G++AFV+ D + +FL RD
Sbjct: 61 KKMQQHFE---FEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRD 117
Query: 138 RFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGI 196
+G++PL+ + SE KG++ L H+ F + +
Sbjct: 118 TYGVRPLFKAMTEDGFLAVCSEAKGLVT--------------------LKHSATPFLK-V 156
Query: 197 KRLMPGYNAVFDLNTR----KFEAWKY-----------YDLRERIEKRKEISDPQG-FKD 240
+ +PG+ V DL E KY YD E++ EI + +
Sbjct: 157 EPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLFPGFEIETVKNNLRI 216
Query: 241 LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIK--VFSLVFPGKEIDESK 298
LF AVK RL+ D +G LSGGLDSS + + L + +++ + + ++ +
Sbjct: 217 LFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLL 276
Query: 299 YQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIY----GQYRVMK-LARE 353
R V D Y FN E+ + + ++I + E TL + G Y + K + R
Sbjct: 277 AARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLE--LMTLQQFVLRIGMYLISKYIGRT 334
Query: 354 NGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMV 413
++ G+GSDE+ GY YF E + + +LLRE
Sbjct: 335 QIAWLIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESERLLRE------------------ 375
Query: 414 LYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLL 473
LY+ V R LR F++ + Y SLP +
Sbjct: 376 LYLFDVLRADRTTAAHGLELRVPFLDH--------------------RFSSYYLSLPPEM 415
Query: 474 RFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNR 533
R KN + + R F D L+ + P+ S GIT V + IL + + ++
Sbjct: 416 RI-PKNGIEKHL-LRETFEDSNLIPKEILWRPKEAFSDGITSV--KNSWFKILQEYVEHQ 471
Query: 534 ASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQE 593
D + N QKF + K + + YR+V FE H G+ +W
Sbjct: 472 VD---------DAMMANAAQKFPF-----NTPKTKEGYYYRQV---FERHYPGRADWLSH 514
Query: 594 LW 595
W
Sbjct: 515 YW 516
>sp|P19252|ASN2_PEA ASPARAGINE SYNTHETASE, ROOT [GLUTAMINE-HYDROLYZING]
(GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE)
>gi|68660|pir||AJPMN2 asparagine synthase
(glutamine-hydrolyzing) (EC 6.3.5.4) 2 - garden pea
>gi|20652|emb|CAA36430.1| (X52180) asparagine synthase
(glutamine-hydrolysing) [Pisum sativum]
Length = 583
Score = 122 bits (302), Expect = 2e-26
Identities = 115/392 (29%), Positives = 181/392 (45%), Gaps = 54/392 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D KR ++ ++HRGP+ G++ + L RLAI+D + G
Sbjct: 1 MCGILAVLGCSDPSRAKRVRVLELSRRLKHRGPEWSGLHQHGDCYLAQQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ + +D + + NGEIYN ++RK+L +TF + +D +VI Y E+G D V+
Sbjct: 60 DQPL-FNEDNPSI-VTVNGEIYNHEDLRKQLSN--HTFRTGSDCDVIAHLYEEYGEDFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV D N ++RD G+ LY + DG ++ SSE+KG+ +
Sbjct: 116 MLDGIFSFVPLDTRDNSYIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL--------ND 166
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D F+ L ++D+ F R++ +Y E I
Sbjct: 167 DCEHFECFPPGHLYSSKDSGF------------------RRWYNPSWYS--EAIPSAP-- 204
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFS 286
DP + F +AV RL+ DVP G LSGGLDSS + L + K+ S
Sbjct: 205 YDPLALRHAFEKAVVKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHS 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
+ + K + V D + TF V+D + I D+I E + SI
Sbjct: 265 FCVGLEGSPDLKAGKEVADYLGTVHHEFTFTVQDGIDAIEDVI-YHVETYDVTSIRASTP 323
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K ++ G+GSDEI GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355
>dbj|BAB17726.1| (AB050900) asparagine synthetase [Raphanus sativus]
Length = 585
Score = 121 bits (301), Expect = 2e-26
Identities = 118/392 (30%), Positives = 175/392 (44%), Gaps = 53/392 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ +RHRGPD G+Y + L H RLAIID + G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGIYQNGGNYLAHQRLAIIDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+RK L K + F + +D +VI Y E G + V+
Sbjct: 60 DQPLFNED--KSIVVTVNGEIYNHEELRKGL--KNHKFHTGSDCDVIAHLYEEHGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAVGVTSLYIGWGLDG-SLWVSSEMKGL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
H + FE P + + F+ W Y
Sbjct: 165 --------------HEDCEHFEA----FPPGHLYSSKSGGGFKQW-YNPPWFNESVPSTP 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VFS 286
+P + F AV RL+ DVP G LSGGLDSS + RHL K + S
Sbjct: 206 YEPLAIRRAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKRWGPQLHS 265
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
+ + K + V + + F V+D + I D+I E + +I
Sbjct: 266 FCVGLEGSPDLKAGKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 324
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEI GY YF
Sbjct: 325 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 356
>emb|CAA08913.1| (AJ009952) asparagine synthetase type II [Phaseolus vulgaris]
Length = 584
Score = 121 bits (300), Expect = 3e-26
Identities = 118/395 (29%), Positives = 178/395 (44%), Gaps = 60/395 (15%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDHSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCFLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K V + NGEIYN E+RK+L G+ F + +D +VI Y E G V+
Sbjct: 60 DQPLFNED--KSVIVTVNGEIYNHEELRKQLP--GHKFRTGSDCDVIAHLYEEHGEGFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
D E + PG+ ++ R F W Y+ E I
Sbjct: 166 DDC------------------EHFECFPPGH--LYSSKDRGFRRW--YNPPWFSEAIPSA 203
Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
DP + F +AV RL+ VP G LSGGLDSS + L + + K
Sbjct: 204 P--YDPLVLRRAFEQAVIKRLMTGVPFGVLLSGGLDSSLVASITSRYLANTKAAEQWGSK 261
Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
+ S + + K + V D + F V+D + I D+I E + +I
Sbjct: 262 LHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRA 320
Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K ++ G+GSDEI GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355
>sp|P49091|ASNS_BRAOL ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|1084354|pir||S52387
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
- wild cabbage >gi|669057|emb|CAA59138.1| (X84448)
asparagine synthase (glutamine-hydrolysing) [Brassica
oleracea]
Length = 586
Score = 121 bits (300), Expect = 3e-26
Identities = 118/392 (30%), Positives = 174/392 (44%), Gaps = 53/392 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D+ KR ++ +RHRGPD G+Y + L H RLAIID G
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGIYQNGFNYLAHQRLAIID-PDSG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K + + NGEIYN E+RK L K + F + +D +VI Y E G + V+
Sbjct: 60 DQPLFNED--KSIVVTVNGEIYNHEELRKGL--KNHKFHTGSDCDVIAHLYEEHGENFVD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+++FV+ D N ++RD G+ LY + DG ++ SSE+KG+
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAVGVTSLYIGWGLDG-SLWVSSEMKGL---------- 164
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
H + FE P + + F+ W Y
Sbjct: 165 --------------HEDCEHFEA----FPPGHLYSSKSGGGFKQW-YNPPWFNESVPSTP 205
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VFS 286
+P + F AV RL+ DVP G LSGGLDSS + RHL K + S
Sbjct: 206 YEPLAIRSAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKRWGPQLHS 265
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
+ + K + V + + F V+D + I D+I E + +I
Sbjct: 266 FCVGLEGSPDLKAGKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 324
Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+ ++R+ G+K++L G+GSDEI GY YF
Sbjct: 325 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 356
>sp|P17714|ASNS_MESAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE) >gi|68658|pir||AJHYNG asparagine
synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - golden
hamster >gi|49614|emb|CAA36375.1| (X52130) asparagine
synthetase (AA 1-561) [Mesocricetus auratus]
Length = 561
Score = 120 bits (297), Expect = 6e-26
Identities = 153/629 (24%), Positives = 255/629 (40%), Gaps = 126/629 (20%)
Query: 1 MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI D+ L + A++ HRGPD E V N G RLA++D G
Sbjct: 1 MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
QP++ +K +W+ YNGEIYN +++ E + + +N D E+IL Y + G + +
Sbjct: 60 QPIRVKKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEQTIC 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGI--LQHN----- 166
+G++AF++ D + +FL RD +G++PL+ + SE KG+ L+H+
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFL 175
Query: 167 ------------IPRKPNDAV--IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR 212
+ KPN V + Y++ D ++ +++L PG F+L T
Sbjct: 176 KVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHALYDSVEKLFPG----FELETV 231
Query: 213 KFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA 272
K + LF AV+ RL+ D + LSGGLDSS + +
Sbjct: 232 K----------------------SNLRILFDNAVRKRLMTDRRIVCLLSGGLDSSLVASS 269
Query: 273 MRHLLPDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIID 327
+ L + +++ + + ++ + R V + + FN E+ L ++I
Sbjct: 270 LLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIF 329
Query: 328 LIRTQEEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRK 386
+ T + S+ G Y + K R+N V+ G+GSDE+ GY YF E +
Sbjct: 330 SLETYDITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE 388
Query: 387 FKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPS 446
+ +LL+E+ + + + + R P+L F + P
Sbjct: 389 -ESERLLKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPP 434
Query: 447 KELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPE 506
+ + K E LL ET FED N + I R P+
Sbjct: 435 EMRV--PKNGIEKHLLRET---------FEDSNLLPKEILWR----------------PK 467
Query: 507 SKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFK 566
S GIT V + IL D + ++ D + QKF + K
Sbjct: 468 EAFSDGITSV--KNSWFKILQDYVEHQVD---------DAMMATAAQKFPF-----NTPK 511
Query: 567 KRPYWDYRKVLRMFEEHVSGKKNWSQELW 595
+ + YR++ FE H G+ +W W
Sbjct: 512 TKEGYFYRQI---FEHHYPGRADWLTHYW 537
>gi|6325403 asparagine synthetase; Asn1p [Saccharomyces cerevisiae]
>gi|1351984|sp|P49089|ASN1_YEAST ASPARAGINE SYNTHETASE
[GLUTAMINE-HYDROLYZING] 1 (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE 1) >gi|1077018|pir||S52694
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
ASN1 - yeast (Saccharomyces cerevisiae)
>gi|747902|emb|CAA88594.1| (Z48675) asparagine
synthetase [Saccharomyces cerevisiae]
>gi|1066479|gb|AAB68284.1| (U40829) Similar to
Asparagine synthetase B from E. coli (Swiss Prot.
accession number P22106) and other species
[Saccharomyces cerevisiae]
Length = 572
Score = 113 bits (280), Expect = 6e-24
Identities = 116/415 (27%), Positives = 179/415 (42%), Gaps = 75/415 (18%)
Query: 1 MCGI-NGFSWGDEELVK----RMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI F D K +++ IRHRGPD G + ++ H RLAI+ + G
Sbjct: 1 MCGIFAAFRHEDVHRYKPKALQLSKRIRHRGPDWSGNAIKNSTIFVHERLAIVGVE-SGA 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVER 115
QP+ DG E + NGEIYN +++R+E + Y F + +D E I+ YL+ D +
Sbjct: 60 QPIT-SSDG-EYMLCVNGEIYNHIQLREECAD--YEFGTLSDCEPIIPMYLKHDIDAPKY 115
Query: 116 FNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDG---KNIIFSSEIKGILQHNIPRKPN 172
+GM+A+ +YD ++ + +RD GI LY K + F+SE+K +
Sbjct: 116 LDGMFAWTLYDAKQDRIVAARDPIGITTLYMGRSSASPKTVYFASELKCL---------- 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
T+D + I PG+ V+D T K + D +
Sbjct: 166 ---------------TDDC--DTITAFPPGH--VYDSKTDKITRYFTPDWLDEKRIPSTP 206
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV--------------------CA 272
D + +AV+ RL+A+VP G LSGGLDSS I
Sbjct: 207 IDYMAIRHSLEKAVRKRLMAEVPYGVLLSGGLDSSLIASIAARETAKATNDVEPSTYDSK 266
Query: 273 MRHLL---PDGEI------KVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLA 323
RHL DG++ + S + + R V + TF +++ L
Sbjct: 267 ARHLAGIDDDGKLHTAGWTSLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGL- 325
Query: 324 DIIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
D +D + E + +I + L+R+ G+K++L G+GSDEI GY YF
Sbjct: 326 DALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYF 380
>gi|6321563 asparagine synthetase; Asn2p [Saccharomyces cerevisiae]
>gi|1351986|sp|P49090|ASN2_YEAST ASPARAGINE SYNTHETASE
[GLUTAMINE-HYDROLYZING] 2 (GLUTAMINE-DEPENDENT
ASPARAGINE SYNTHETASE 2) >gi|1084549|pir||S55982
asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
ASN2 - yeast (Saccharomyces cerevisiae)
>gi|642347|emb|CAA58159.1| (X83099) glutamic-dependent
asparagine synthase [Saccharomyces cerevisiae]
>gi|1323203|emb|CAA97135.1| (Z72909) ORF YGR124w
[Saccharomyces cerevisiae]
Length = 572
Score = 113 bits (280), Expect = 6e-24
Identities = 114/414 (27%), Positives = 173/414 (41%), Gaps = 74/414 (17%)
Query: 1 MCGI-NGFSWGDEELVK----RMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGH 55
MCGI F D K +++ IRHRGPD G V ++ H RLAI+ L G
Sbjct: 1 MCGIFAAFKHEDIHNFKPKALQLSKKIRHRGPDWSGNAVMNSTIFVHERLAIVGLD-SGA 59
Query: 56 QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVER 115
QP+ DG E + NGEIYN +++R+ + Y F + +D E I+ YLE D +
Sbjct: 60 QPIT-SADG-EYMLGVNGEIYNHIQLREMCSD--YKFQTFSDCEPIIPLYLEHDIDAPKY 115
Query: 116 FNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKN---IIFSSEIKGILQHNIPRKPN 172
+GM+AF +YD K+ + +RD G+ LY ++ + F+SE+K +
Sbjct: 116 LDGMFAFCLYDSKKDRIVAARDPIGVVTLYMGRSSQSPETVYFASELKCLTD-------- 167
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
+ I PG+ V+D T K + D +
Sbjct: 168 -------------------VCDSIISFPPGH--VYDSETDKITRYFTPDWLDEKRIPSTP 206
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA-------------------- 272
D + +AV+ RL+A+VP G LSGGLDSS I
Sbjct: 207 VDYHAIRHSLEKAVRKRLMAEVPYGVLLSGGLDSSLIAAIAARETEKANADANEDNNVDE 266
Query: 273 --------MRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLAD 324
HL G ++ S + + R V + TF +++ L D
Sbjct: 267 KQLAGIDDQGHLHTSGWSRLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGL-D 325
Query: 325 IIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
+D + E + +I + L+R+ G+K++L G+GSDEI GY YF
Sbjct: 326 ALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYF 379
>gb|AAC49614.1| (U77679) asparagine synthetase 1 [Glycine max]
Length = 579
Score = 113 bits (280), Expect = 6e-24
Identities = 110/392 (28%), Positives = 171/392 (43%), Gaps = 54/392 (13%)
Query: 1 MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI D KR ++ ++HRGPD G++ + L H RLAI+D + G
Sbjct: 1 MCGILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGDNYLAHQRLAIVDPA-SG 59
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ E K V + NGEIYN E+RK+L +TF + +D +VI Y E G + ++
Sbjct: 60 DQPLFNED--KTVVVTVNGEIYNHEELRKQLPN--HTFRTGSDCDVIAHLYEEHGENFMD 115
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
+G+ +FV+ D N ++RD G+ LY + DG ++ SSE+KG+ N
Sbjct: 116 MLDGISSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSELKGL---------N 165
Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
D E + PG+ ++ R F W Y +
Sbjct: 166 DDC------------------EHFESFPPGH--LYSSKERAFRRW-YNPPWLSLAIPSAP 204
Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
DP + F + RL+ DVP G LSGGLDSS + L + K+ S
Sbjct: 205 YDPLALRHAFEKLWIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGTKLHS 264
Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYG 343
+ + K + V + + + V+ D + D+I I T + SI
Sbjct: 265 FCVGLEGAPDLKATKEVAEYIGTVHHEFHYTVQDGIDAIEDVIYHIETYDVTTIRASI-P 323
Query: 344 QYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
+ + + + G+K ++ G+GSD GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDVFFGGYLYF 355
>pir||T18441 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) C0395w
[similarity] - malaria parasite (Plasmodium falciparum)
>gi|3649765|emb|CAB11114.1| (Z98547) predicted using
hexExon; MAL3P3.14 (PFC0395w), Asparagine synthetase,
len: 611 aa; Similarity to many asparagine synthetases.
O.sativa asparagine synthetase (SW:ASNS_ORYSA) BLAST
Score: 1003, sum P(2) = 5.5e-165; 65% identity in 290 aa
overlap
Length = 610
Score = 113 bits (279), Expect = 8e-24
Identities = 126/414 (30%), Positives = 181/414 (43%), Gaps = 94/414 (22%)
Query: 18 MNDAIRHRGPDDEGVYVDDNVS-----LGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYN 72
++ +RHRGPD G+ V++N L H RLAI+D+ GHQP+ Y+ D +EV + N
Sbjct: 24 LSKILRHRGPDWNGIVVEENDDGTTNVLAHERLAIVDVL-SGHQPL-YD-DEEEVCLTIN 80
Query: 73 GEIYNFMEIRKELEEKGYT-FTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNI 131
GEIYN +E+RK ++E+ S +D VI + + +G++A VI DK N
Sbjct: 81 GEIYNHLELRKLIKEENLNKLKSCSDCAVIPNLFKIYKEKIPSMLDGIFAGVISDKKNNT 140
Query: 132 LFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTE 189
F RD GI PLY Y DG +I FSSE K + + I VIF
Sbjct: 141 FFAFRDPIGICPLYIGYAADG-SIWFSSEFKALKDNCI-----RYVIFP----------- 183
Query: 190 DTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD-----LRERIEKRKEISDPQGFKDLFLR 244
PG+ + N K E +YY+ L I K D + +
Sbjct: 184 -----------PGH--YYKNNKNKGEFVRYYNPNWWSLNNSIPNNK--VDFNEIRIHLEK 228
Query: 245 AVKMRLVADVPVGSCLSGGLDSSSIVCAM-RHL----------LPDG------------- 280
AV RL+ DVP G LSGGLDSS I + +HL + +G
Sbjct: 229 AVIKRLMGDVPFGILLSGGLDSSIIAAILAKHLNILDNKNGKSIQNGNDKKSNNNGNESN 288
Query: 281 ----------------EIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DV 321
+++ FS+ G + K + V + + F VE D
Sbjct: 289 NNNNNNNFNNNNSGPQKLRSFSIGLKGS--PDLKAAKEVAEYLGIEHTEFYFTVEEGIDS 346
Query: 322 LADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
L D+I I T + S Y + +L + + +K++L G+GSDEI GY YF
Sbjct: 347 LHDVIYHIETYDITTIRAST-PMYILSRLIKSSCVKMVLSGEGSDEIFGGYLYF 399
>pir||T42010 probable asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) -
fission yeast (Schizosaccharomyces pombe) (fragment)
>gi|1749410|dbj|BAA13764.1| (D89101) similar to
Saccharomyces cerevisiae Asparagine
synthetase(glutamine-hydrolyzing)2, SWISS-PROT Accession
Number P49090 [Schizosaccharomyces pombe]
Length = 559
Score = 102 bits (253), Expect = 9e-21
Identities = 93/281 (33%), Positives = 137/281 (48%), Gaps = 48/281 (17%)
Query: 1 MCGINGFSWGDEEL------VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
MCGI E++ ++ +RHRGPD G + + L H RLAI+ + G
Sbjct: 3 MCGILAVHHVAEDIEAFKPKALHLSKQLRHRGPDWSGKAIRNQTILCHERLAIVGVE-SG 61
Query: 55 HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
QP+ + DGK V V NGEIYN +++R+ L+ Y F + +D EVIL Y E G C
Sbjct: 62 AQPLVSD-DGKLVLTV-NGEIYNHLKLRENLKGN-YKFKTYSDCEVILYLYREHGPACAN 118
Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKN---IIFSSEIKGILQHNIPRKP 171
+GM+++V+YD+ K+ + +RD GI LY + + F+SE+K + H + K
Sbjct: 119 MLDGMFSWVLYDQDKDKVVAARDPIGITTLYQGFSSDSPDTAYFASELKAL--HPVCDK- 175
Query: 172 NDAVIFDF---LYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEK 228
I F YY+ T+ T ++ P + +D N Y LRE +E
Sbjct: 176 ----IIAFPPGHYYD--SETKQT----VRYFKPSW---WDENKIPSNPVDYKLLRETLE- 221
Query: 229 RKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSI 269
+V+ RL+A+VP G LSGGLDSS I
Sbjct: 222 ---------------ASVRKRLMAEVPYGVLLSGGLDSSLI 247
>pir||T16625 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) M02D8.4
[similarity] - Caenorhabditis elegans
>gi|1086745|gb|AAA82381.1| (U41034) coded for by C.
elegans cDNA yk61f9.5; coded for by C. elegans cDNA
yk80c1.5; coded for by C. elegans cDNA yk4g5.5; coded
for by C. elegans cDNA yk127h1.5; coded for by C.
elegans cDNA yk37d7.5; coded for by C. elegans cDNA
yk55e11.5; coded fo>
Length = 567
Score = 101 bits (250), Expect = 2e-20
Identities = 106/376 (28%), Positives = 173/376 (45%), Gaps = 51/376 (13%)
Query: 11 DEELVKRMNDAIRHRGPDDEGVYVDDNVS--LGHVRLAIIDLSPKGHQPMKYEKDGKEVW 68
D + ++++ HRGPD G Y D L H RLAI+DL QP++ ++V
Sbjct: 19 DLDEARKLSRRQHHRGPDFSGCYQDTKTGDILVHERLAIMDLGVV--QPLQGTAPNRQV- 75
Query: 69 IVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEW--GFDCVERFNGMWAFVIYD 126
++NGEIYN E+R + E K Y ++ D+EVI+ Y ++ G C +G++AFV+
Sbjct: 76 -IHNGEIYNHQELR-DTELKKYNLKTHCDSEVIIFLYEKYRDGHIC-NLLDGVFAFVLC- 131
Query: 127 KSKNILFLSRDRFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLL 185
+RD G+K +YY D FS+E+K +
Sbjct: 132 -CDGDFLAARDPLGVKQMYYGIDDDGRYFFSNEMKCL----------------------- 167
Query: 186 DHTEDTFFEG-IKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLR 244
ED+ + I+ PG+ ++ +++D R+ I D + + +
Sbjct: 168 ---EDSCGDNKIESFPPGHYYTPKTGFVRYFNPEWFDFRKAIHP----LDLKLLQKTMIA 220
Query: 245 AVKMRLVADVPVGSCLSGGLDSSSI-VCAMRHLLPDG-EIKVFSLVFPGKEIDESKYQRI 302
+V RL++D P+G LSGGLDSS + A R + G + FS+ D R
Sbjct: 221 SVHKRLMSDAPIGVLLSGGLDSSLVSSIASREMKRRGMAVHSFSIGVDHNSPDVVA-ARK 279
Query: 303 VVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG---QYRVMKLARENGMKVL 359
V + + F++E+ + ++ LI E + SI Y + + R+ G+KV+
Sbjct: 280 VAKFIGTTHHEFYFSIEEGIKNLRKLI-WHLESYDVTSIRASTPMYFLSEEIRKLGIKVV 338
Query: 360 LDGQGSDEILAGYHYF 375
L G+G+DEI GY YF
Sbjct: 339 LSGEGADEIFGGYLYF 354
>pir||G75092 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) PAB0750
[similarity] - Pyrococcus abyssi (strain Orsay)
>gi|5458556|emb|CAB50044.1| (AJ248286) asparagine
synthetase, putative [Pyrococcus abyssi]
Length = 479
Score = 100 bits (247), Expect = 4e-20
Identities = 117/401 (29%), Positives = 179/401 (44%), Gaps = 77/401 (19%)
Query: 1 MCGI-NGFSWGDEELVKRMNDAIRHRGPDDEGVYVD------DNVSL------GHVRLAI 47
MC I GF+ E+ +K +N +HRGPD G + + DN S G + L
Sbjct: 1 MCLIAGGFNVSREDAIKIINLG-KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQ 59
Query: 48 IDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLE 107
L+ G + + E+ +V+NGEIYN +RK L E+G F ++ D+EVIL
Sbjct: 60 CRLAMTGSKSYT-QPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEY 118
Query: 108 WGFD-----------CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFS 156
FD ++ G +A + K LF RD G++PLYY G F+
Sbjct: 119 LVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLYYSSRGH---FA 173
Query: 157 SEIK---GILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRK 213
SE K G+ + P P + V ++ H GI R+ G+N VF + T
Sbjct: 174 SEKKVLWGLGEDAEPVLPGEIV-------SISKH-------GITRVR-GFN-VFSIRT-- 215
Query: 214 FEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAM 273
+ Y++ G ++L + +VK+R A VG SGG+DSS I
Sbjct: 216 -QGKDYFN---------------GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIA--- 254
Query: 274 RHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQE 333
L+ V ++ + ++ R V +E + + F+ ED+ +I ++ E
Sbjct: 255 --LIASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIE 312
Query: 334 E--PFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
E P + Y K ARE+G+KVLL GQG+DE+ GY
Sbjct: 313 EPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGY 353
>pir||G71050 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) PH1102
[similarity] - Pyrococcus horikoshii
>gi|3257518|dbj|BAA30201.1| (AP000005) 480aa long
hypothetical asparagine synthetase [Pyrococcus
horikoshii]
Length = 480
Score = 98.3 bits (241), Expect = 2e-19
Identities = 119/406 (29%), Positives = 178/406 (43%), Gaps = 87/406 (21%)
Query: 1 MCGI-NGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVS-----------------LGH 42
MC I GF +E++ R+ + +HRGPD GV+ + V L
Sbjct: 1 MCLIAGGFKLYTDEII-RIIELGKHRGPDSFGVWSEREVIKSSDFSALEEVKGGDKVLIQ 59
Query: 43 VRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVIL 102
RLA+ S + QP E+ +V+NGEIYN ++R L E+G +F S+ D+EVIL
Sbjct: 60 CRLAMTG-SKEYTQPFY-----NEIALVHNGEIYNHEKLRAYLVERGISFESDVDSEVIL 113
Query: 103 AAYLEWGFD-----------CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGK 151
FD + G +A + K LF RD GI+PLYY G
Sbjct: 114 RLLEYLLFDRNLSPWNAIKKLMWMLRGDYAVAFHYNGKFYLF--RDPLGIRPLYYSSQGH 171
Query: 152 NIIFSSEIK---GILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFD 208
F SE K G+ + P P +AV E + F GI R+ ++D
Sbjct: 172 ---FGSEKKVLWGLGEEATPVMPGEAV-------------EISKF-GINRV-----RLYD 209
Query: 209 LNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSS 268
+ K +Y+D G ++L + +VK+R + +G SGGLDS+
Sbjct: 210 VTAIKSFGSEYFD---------------GLRNLLIDSVKIR--SSKKIGILFSGGLDSTL 252
Query: 269 IVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDL 328
I + V V ++ + ++ R V E + F+ E+V ++ +
Sbjct: 253 IA-----FIASRYSNVTLYVSGTEDSKDVEWARKVSQELGLKLKEYIFSKEEVEKELKRI 307
Query: 329 IRTQEEPF-STLSI-YGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
+ EEP LSI Y KLARE+G+K+LL GQG+DE+ GY
Sbjct: 308 MFAIEEPNPMNLSIAIPLYFSTKLAREDGVKILLSGQGADELFGGY 353
>emb|CAA62733.1| (X91388) unknown [Pseudomonas syringae]
Length = 433
Score = 84.7 bits (206), Expect = 3e-15
Identities = 95/383 (24%), Positives = 155/383 (39%), Gaps = 29/383 (7%)
Query: 13 ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYN 72
+++ M A+ RGPD + V++ +++LGH + QP E G V I+Y
Sbjct: 27 KVILDMIAAMAGRGPDGQAVWLGPDLALGHCS-TVASRYDFNEQPWIGEIRGATVAIMYT 85
Query: 73 GEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNIL 132
G+IYN ++++EL G + E+I A L G+ + + D K L
Sbjct: 86 GQIYNMWQLQQELASFGPAPDRWSYAEIIRAR-LWRGYGTARSALRACSLLPLDGQKREL 144
Query: 133 FLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTF 192
L+RDR G++PLYY IIF+S I H D + + +T
Sbjct: 145 LLARDRLGLQPLYYTLTDGGIIFASVPTAIQAHPQFTAKLDRSALAIVLQPRMAMPGETP 204
Query: 193 FEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVA 252
+G+ L P V + R +Y+ L + L V +
Sbjct: 205 LKGLHELPPA--NVLSFSKRGHTQVRYWQLESAPHTDNFEQTSDHVRALLEGVVARQFED 262
Query: 253 DVPVGSCLSGGLDSSSIVC-AMRHLLPDG---EIKVFSLVFPGKEID----------ESK 298
VP + LSGG+DS+S+ M+ L G + F L F E ++
Sbjct: 263 QVPSAAMLSGGIDSTSVAALVMKQLATRGAGPALDTFCLKFDTDEDAFTSSELRPDIDAP 322
Query: 299 YQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ-----YRVMKLARE 353
+ + + + ++D+L ++I RT L +GQ Y + + R+
Sbjct: 323 FAALAAEYMGTRHTTLSATMQDLL-NVIPATRTAR----GLPGWGQFDASMYLLFERMRD 377
Query: 354 NGMKVLLDGQGSDEILAGYHYFF 376
N V L G+ +DEI GY Y+F
Sbjct: 378 N-FSVALTGEAADEIFGGYPYYF 399
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.323 0.142 0.438
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 250008298
Number of Sequences: 2977
Number of extensions: 11224040
Number of successful extensions: 29887
Number of sequences better than 1.0e-10: 80
Number of HSP's better than 0.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 62
Number of HSP's that attempted gapping in prelim test: 29463
Number of HSP's gapped (non-prelim): 199
length of query: 611
length of database: 189,106,746
effective HSP length: 53
effective length of query: 558
effective length of database: 157,386,935
effective search space: 87821909730
effective search space used: 87821909730
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 168 (69.9 bits)