BLASTP 2.0.10 [Aug-26-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= PAB1605 (PAB1605) DE:PUTATIVE ASPARAGINE SYNTHETASE
(GLUTAMINE-HYDROLYZING) (EC 6.3.5.4)
         (611 letters)

Database: ./suso.pep; /banques/blast2/nr.pep
           598,487 sequences; 189,106,746 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||F75095 probable asparagine synthase (glutamine-hydrolyzing)...  1269  0.0
emb|CAA07658.1| (AJ007747) putative glutamine amidotransferase [...   351  2e-95
gb|AAB99058.1| (U67548) asparagine synthetase (asnB) [Methanococ...   292  9e-78
emb|CAA07652.1| (AJ007747) putative glutamine amidotransferase [...   269  6e-71
pir||S70877 hypothetical protein 2 - Vibrio cholerae >gi|1230578...   256  4e-67
sp|P54420|ASNB_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   256  4e-67
dbj|BAA33606.1| (AB012956) probable asparagine synthetase [Vibri...   255  1e-66
emb|CAA69108.1| (Y07786) ORF71x8 [Vibrio cholerae]                    254  2e-66
gb|AAF34252.1|AF168003_7 (AF168003) putative asparagine syntheta...   250  3e-65
dbj|BAB05227.1| (AP001512) asparagine synthetase [Bacillus halod...   242  7e-63
sp|O05272|ASNO_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   240  3e-62
pir||F69837 asparagine synthase homolog yisO - Bacillus subtilis...   232  8e-60
pir||F83384 probable asparagine synthetase PA2084 [imported] - P...   228  2e-58
gb|AAF24002.1| (AF035937) WbpS [Pseudomonas aeruginosa]               226  8e-58
emb|CAB58327.1| (AL121855) putative amidotransferase [Streptomyc...   220  3e-56
pir||A83638 probable glutamine amidotransferase PA0051 [imported...   210  4e-53
pir||B83214 probable glutamine amidotransferase PA3459 [imported...   202  9e-51
dbj|BAB12569.1| (AB039379) asparagine synthase homolog [Streptom...   201  3e-50
dbj|BAA89484.1| (AB029550) LtsA [Corynebacterium glutamicum]          196  5e-49
gb|AAF17502.1|AF195615_10 (AF195615) PhzH [Pseudomonas chlororap...   192  7e-48
sp|Q10374|ASNH_MYCTU PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-H...   180  4e-44
gb|AAG02363.1|AF210249_22 (AF210249) asparagine synthetase [Stre...   176  8e-43
sp|P42113|ASNH_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   170  4e-41
pir||H82255 asparagine synthetase B, glutamine-hydrolyzing VC099...   143  4e-33
sp|P22106|ASNB_ECOLI ASPARAGINE SYNTHETASE B [GLUTAMINE-HYDROLYZ...   139  1e-31
pdb|1CT9|A Chain A, Crystal Structure Of Asparagine Synthetase B...   139  1e-31
dbj|BAA89376.1| (AB025342) ORF2 [Moritella marina]                    137  3e-31
gb|AAB99117.1| (U67554) asparagine synthetase (asnB) [Methanococ...   136  1e-30
sp|Q58516|ASNH_METJA PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-H...   136  1e-30
emb|CAA96526.1| (Z72354) asparagine synthetase [Vicia faba]           135  2e-30
gb|AAF02775.1|AF190728_1 (AF190728) asparagine synthetase [Helia...   133  5e-30
dbj|BAA96252.1| (AB035248) asparagine synthetase [Astragalus sin...   133  5e-30
gb|AAF02776.1|AF190729_1 (AF190729) asparagine synthetase [Helia...   133  6e-30
dbj|BAA97313.1| (AB019236) asparagine synthetase [Arabidopsis th...   132  1e-29
gb|AAC72837.1| (AF095453) asparagine synthetase [Arabidopsis tha...   132  1e-29
gi|4502259 asparagine synthetase [Homo sapiens] >gi|114266|sp|P0...   131  2e-29
sp|O24661|ASNS_TRIVS ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   131  3e-29
gi|11421677 asparagine synthetase [Homo sapiens] >gi|3341715|gb|...   130  4e-29
gb|AAC72836.1| (AF095452) asparagine synthetase [Arabidopsis tha...   130  4e-29
pir||T08846 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   129  1e-28
pir||D82846 asparagine synthase B XF0118 [imported] - Xylella fa...   129  1e-28
gb|AAB48058.1| (L40327) asparagine synthetase [Medicago sativa]       129  1e-28
gb|AAB81011.1| (U89923) asparagine synthetase [Medicago sativa]       129  1e-28
pir||T50812 asparagine synthetase ASN3 - Arabidopsis thaliana >g...   128  2e-28
sp|P49094|ASNS_MAIZE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   128  2e-28
sp|P49078|ASNS_ARATH ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   128  2e-28
gb|AAF74755.1| (AF263432) asparagine synthetase [Helianthus annuus]   128  2e-28
dbj|BAA96251.1| (AB035247) asparagine synthetase [Astragalus sin...   128  2e-28
gb|AAC16325.1| (AF061740) asparagine synthetase [Elaeagnus umbel...   128  2e-28
sp|P49093|ASN2_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   128  2e-28
sp|Q43011|ASNS_ORYSA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   127  4e-28
sp|P31752|ASNS_ASPOF ASPARAGINE SYNTHASE [GLUTAMINE-HYDROLYZING]...   127  5e-28
emb|CAA67889.1| (X99552) asparagine synthetase [Asparagus offici...   127  5e-28
sp|P19251|ASN1_PEA ASPARAGINE SYNTHETASE, NODULE [GLUTAMINE-HYDR...   127  5e-28
sp|P49092|ASN1_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   126  6e-28
pir||JQ2245 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   125  1e-27
sp|Q61024|ASNS_MOUSE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   125  1e-27
sp|O24338|ASNS_SANAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   125  1e-27
prf||2207183A Asn synthetase [Rattus norvegicus]                      125  2e-27
pir||JW0071 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   124  3e-27
pir||T39308 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   124  3e-27
sp|P19891|ASNS_CRIGR ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   123  5e-27
sp|P49088|ASNS_RAT ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]...   123  7e-27
gb|AAB95197.1| (U84118) asparagine synthetase [Aedes aegypti]         123  7e-27
emb|CAB57292.1| (AJ133522) asparagine synthetase (type-I) [Phase...   123  7e-27
pir||AJHUN2 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   122  1e-26
sp|P19252|ASN2_PEA ASPARAGINE SYNTHETASE, ROOT [GLUTAMINE-HYDROL...   122  2e-26
dbj|BAB17726.1| (AB050900) asparagine synthetase [Raphanus sativus]   121  2e-26
emb|CAA08913.1| (AJ009952) asparagine synthetase type II [Phaseo...   121  3e-26
sp|P49091|ASNS_BRAOL ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   121  3e-26
sp|P17714|ASNS_MESAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZIN...   120  6e-26
gi|6325403 asparagine synthetase; Asn1p [Saccharomyces cerevisia...   113  6e-24
gi|6321563 asparagine synthetase; Asn2p [Saccharomyces cerevisia...   113  6e-24
gb|AAC49614.1| (U77679) asparagine synthetase 1 [Glycine max]         113  6e-24
pir||T18441 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   113  8e-24
pir||T42010 probable asparagine synthase (glutamine-hydrolyzing)...   102  9e-21
pir||T16625 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   101  2e-20
pir||G75092 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....   100  4e-20
pir||G71050 asparagine synthase (glutamine-hydrolyzing) (EC 6.3....    98  2e-19
emb|CAA62733.1| (X91388) unknown [Pseudomonas syringae]                85  3e-15

>pir||F75095 probable asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           PAB1605 - Pyrococcus abyssi (strain Orsay)
           >gi|5458579|emb|CAB50067.1| (AJ248286) PUTATIVE
           ASPARAGINE SYNTHETASE (GLUTAMINE-HYDROLYZING) (EC
           6.3.5.4) [Pyrococcus abyssi]
           Length = 611
           
 Score = 1269 bits (3248), Expect = 0.0
 Identities = 611/611 (100%), Positives = 611/611 (100%)

Query: 1   MCGINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKY 60
           MCGINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKY
Sbjct: 1   MCGINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKY 60

Query: 61  EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMW 120
           EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMW
Sbjct: 61  EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMW 120

Query: 121 AFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFL 180
           AFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFL
Sbjct: 121 AFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFL 180

Query: 181 YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKD 240
           YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKD
Sbjct: 181 YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKD 240

Query: 241 LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQ 300
           LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQ
Sbjct: 241 LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQ 300

Query: 301 RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLL 360
           RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLL
Sbjct: 301 RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLL 360

Query: 361 DGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVW 420
           DGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVW
Sbjct: 361 DGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVW 420

Query: 421 VTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNA 480
           VTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNA
Sbjct: 421 VTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNA 480

Query: 481 MRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFA 540
           MRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFA
Sbjct: 481 MRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFA 540

Query: 541 TPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIM 600
           TPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIM
Sbjct: 541 TPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIM 600

Query: 601 ELWLREWMDNG 611
           ELWLREWMDNG
Sbjct: 601 ELWLREWMDNG 611


>emb|CAA07658.1| (AJ007747) putative glutamine amidotransferase [Bordetella
           bronchiseptica]
           Length = 628
           
 Score =  351 bits (890), Expect = 2e-95
 Identities = 230/643 (35%), Positives = 337/643 (51%), Gaps = 59/643 (9%)

Query: 1   MCGINGFSWG--DEELVKRMNDAIR---HRGPDDEG----VYVDDNVSLGHVRLAIIDLS 51
           MCG+ G  W   DE    R    +R   HRGPDD G          +SLGH RLA+IDLS
Sbjct: 1   MCGVLGAWWRHHDEHEDARFAAGLRKLAHRGPDDRGSEHFAAPHGRLSLGHTRLAVIDLS 60

Query: 52  PKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD 111
             GHQPM  + D   + +V+NGEIYN+ E+R EL + G +F  ++DTEV+LAA+  WG  
Sbjct: 61  AAGHQPMHADAD--RLAVVFNGEIYNYRELRSELRQIGASFRGDSDTEVLLAAWQHWGVA 118

Query: 112 CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKP 171
            + RF GM+AF +YD+ +  L L+RD FGIKP+YY+   + ++F SE+  + Q  +P  P
Sbjct: 119 ALRRFKGMFAFALYDRRQQTLTLARDAFGIKPMYYHAAPERLLFGSELPAV-QALLPHPP 177

Query: 172 --NDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKR 229
             N    +D+L Y   D   DTF  GI++L  G+    DL T +      +D     E R
Sbjct: 178 RLNWQRAYDYLAYGQYDQPPDTFIAGIRQLPAGHLLTLDLRTGQAGEPMAWDDPASQECR 237

Query: 230 KEISDPQGFKDL-------FLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI 282
                P GF++        FL  V++ L +DVP+G+ LSGG+DSS+IVCAMR L PD  I
Sbjct: 238 -----PIGFQEAAEELRARFLDNVRLHLRSDVPIGAALSGGIDSSAIVCAMRWLEPDLPI 292

Query: 283 KVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIY 342
           + FS +       E ++  +V   C  + ++      ++L D+ DLIR Q EP  + SIY
Sbjct: 293 QTFSFISDDTHRSEERWVDLVNQHCGATGHKIHIAPHELLDDLDDLIRVQGEPVGSTSIY 352

Query: 343 GQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRI 402
            QYRV KLARE+G+ V LDGQG+DE+L GY  +       LL   + G   R +    R 
Sbjct: 353 AQYRVFKLAREHGVVVTLDGQGADELLGGYGGYPTERLASLLETGRLGAAARLL----RQ 408

Query: 403 HGSLVPLKNMVLYIL-------------------PVWVTKRLWRRRFPYLREEFIEKFKK 443
            G   P + +    L                   P W+  ++ R +   +RE        
Sbjct: 409 QGRGAPARLLQAAALAAGGRHLANLRHATGRQHAPAWLDAQVLRDQGVTMREPAHAWAAA 468

Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
            P +      + L   L  A  +  L  LLR  D+N+MR+S+ESRVPF   +L E+ LSL
Sbjct: 469 TPLRG-----RRLVNELAHALYHRGLGALLRHGDRNSMRFSVESRVPFLTQDLAEFALSL 523

Query: 504 PPESKVS-AGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDS 562
           P    VS +G TK + RK ++GI+PD I +R  K+GFATP++  ++    Q     ++D+
Sbjct: 524 PERYLVSDSGQTKHIFRKAMRGIVPDSILDRDDKIGFATPEQQWMQATAPQ-MRQWLVDA 582

Query: 563 ESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLR 605
                 P+    +V   F+  ++G   +S + W+ +  + W++
Sbjct: 583 ADI---PFLRNAEVTAQFDAMIAGATPFSWQAWRWVNFQRWMQ 622


>gb|AAB99058.1| (U67548) asparagine synthetase (asnB) [Methanococcus jannaschii]
           Length = 516
           
 Score =  292 bits (739), Expect = 9e-78
 Identities = 191/491 (38%), Positives = 273/491 (54%), Gaps = 51/491 (10%)

Query: 1   MCGINGFSWGDEELVK----RMNDAIRHRGPDDEGVYVDD----NVSLGHVRLAIIDLSP 52
           MCGING     +E++K    +MN AI+HRGPDDEG+++ +    ++ LGHVRLAI+DLS 
Sbjct: 2   MCGINGIIRFGKEVIKEEINKMNKAIKHRGPDDEGIFIYNFKNYSIGLGHVRLAILDLSE 61

Query: 53  KGHQPMKY------------EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEV 100
           KGHQPM Y            E D  ++ IVYNGEIYN++E++++   +  T    TDTEV
Sbjct: 62  KGHQPMGYNVDEDKIIYRDDELDRADIIIVYNGEIYNYLELKEKFNLETET---GTDTEV 118

Query: 101 ILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIK 160
           IL  Y + GFDCV+ FNGMWAF I+DK K ++F SRDR G+KP YYY+DG   IFSSE+K
Sbjct: 119 ILKLYNKLGFDCVKEFNGMWAFCIFDKKKGLIFCSRDRLGVKPFYYYWDGNEFIFSSELK 178

Query: 161 GILQ-HNIPRKPN---DAVIFDFLYYNL-LDHTEDTFFEGIKRLMPGYNAVFDLN---TR 212
           GIL    I +K N   DAV    LY+ L    +  + ++   +L    N +FDL+    R
Sbjct: 179 GILAVKEINKKENINKDAV---ELYFALGFIPSPYSIYKNTFKLEARQNLIFDLDKREIR 235

Query: 213 KFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA 272
           K+  W+  D +   +K+K I +    K L   AVK+R+ +DVPVG+ LSGGLDSS++V  
Sbjct: 236 KYYYWELPDYKPIYDKKKLIEEG---KKLLYDAVKIRMRSDVPVGAFLSGGLDSSTVVGV 292

Query: 273 MRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQ 332
           MR      ++  FS+ F GK  DE+ Y +IVVD  +   +   F   D    I       
Sbjct: 293 MREFTDLSKLHTFSIGFEGK-YDETPYIKIVVDYFKTQHHHYYFKERDFEELIDKYSWIY 351

Query: 333 EEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKL 392
           +EPF   S +  Y+V ++AR+  + V+L G G DE+  GY      Y ++ +RK      
Sbjct: 352 DEPFGDYSGFPTYKVSEMARK-FVTVVLSGDGGDEVFGGYMTHLNGYRMDFIRK------ 404

Query: 393 LREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK---RPSKEL 449
              +  + R+ GS +P+K  +  I  +++ K  +R       E + E  K+   RP    
Sbjct: 405 ---LPKFLRVVGSKLPVKKDLNGIANLYLLKEAFRLSLINPEEFYAESIKEDAIRPEIYK 461

Query: 450 IWKIKTLNEAL 460
            W I+ L   L
Sbjct: 462 KWTIEKLRYCL 472


>emb|CAA07652.1| (AJ007747) putative glutamine amidotransferase [Bordetella
           bronchiseptica]
           Length = 636
           
 Score =  269 bits (681), Expect = 6e-71
 Identities = 204/651 (31%), Positives = 319/651 (48%), Gaps = 63/651 (9%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR-------HRGPDDEGVYVDDNVSLG--HVRLAIIDLS 51
           MCGI G  W   + V  +   ++       HRGPD  G ++D    LG  HVRL+IIDL 
Sbjct: 1   MCGIFGALWHQGKSVSAIEQQLQVGIARQQHRGPDGHGTWIDPQGRLGLAHVRLSIIDLV 60

Query: 52  PKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD 111
             G QPM+         I YNGEIYN++E+R+EL E  +  TS  DTEVIL AY +WG  
Sbjct: 61  -SGAQPMRGNSGNV---ITYNGEIYNYIELRRELGEDSFETTS--DTEVILRAYEKWGER 114

Query: 112 CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKP 171
           CV+R  GM+AF I+D  +  +F++RDRFGIKP YY        F SE+K +    + R  
Sbjct: 115 CVDRLEGMFAFAIWDAKRGEMFVARDRFGIKPFYYACVQGGFYFGSEVKALTPF-LNRVE 173

Query: 172 NDA-VIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVF--DLNTRKFEAWK-YYDLRERIE 227
            DA  + D+  +      E T F G+++L P +      DL  ++ + W+  YDL     
Sbjct: 174 TDANALHDYFAFQFC-LGEKTLFAGVRQLKPAHCGYVGRDLELKQRQYWEVQYDLDWDHT 232

Query: 228 KRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSL 287
           ++  +   +  ++    +V M + +DV VG+ +SGG+DSS +    RH       + F+ 
Sbjct: 233 EKYFV---EQVREKLQHSVDMHMRSDVEVGAYISGGVDSSLVAAMARHGRRSSRFQGFTG 289

Query: 288 VFP-GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
            F  G   DES+Y + + DE  +  ++     +D + DI  +I   ++P +    + QY 
Sbjct: 290 KFALGDAFDESRYAQALADEHDILLHQVDITEDDFVNDIAKVIYHLDQPVAGPGSFPQYA 349

Query: 347 VMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLL--REILAYKRIHG 404
           V +LA+++ +KV+L GQGSDE+  GY  +   Y+ + ++    G +     I+ Y+ I  
Sbjct: 350 VSRLAKDH-VKVVLGGQGSDEMFGGYARYLIAYWEQCIKGALDGTMHDGNYIVTYESIIP 408

Query: 405 SLVPLKNMVLYILPVWVTKRLWRRRFPYLR-----------------EEFIEKFKKRPSK 447
           +L  L+N    I           R   Y R                 E     F     K
Sbjct: 409 NLRTLRNYKPLIQEFMAEGIFDSRDKRYYRLINRSNTFGNVVNWGMFEGIASSFD--DFK 466

Query: 448 ELIWKIKTLNEALLLAETYYS----LPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
           ++ W      E+   + T++     LP LL+ ED+ +M   IESRVPF DH+L+E V ++
Sbjct: 467 QIFWGKNVGKESYFDSITHFDFKTLLPALLQVEDRMSMAHGIESRVPFLDHKLIELVATV 526

Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATP-DKDILKTNEGQKFALAVIDS 562
           P   K   G  K LLR   + +LP  IR R  K+GF  P    + K  + ++F L    S
Sbjct: 527 PANVKFQNGELKRLLRVAFQDMLPQAIRERKDKMGFPVPLQVWMRKGGKAREFILDTFRS 586

Query: 563 ESFKKRPY----WDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLREWMD 609
           ++ + R Y    +D  K++         +  +S+ +W  + +ELW +++ D
Sbjct: 587 QNARSRAYLAPGFDIEKMMEQ-------EGLFSRNIWAFLSLELWQQQFHD 630


>pir||S70877 hypothetical protein 2 - Vibrio cholerae >gi|1230578|gb|AAC46243.1|
           (U47057) orf2 [Vibrio cholerae O139]
           >gi|1588972|prf||2209416B ORF 2 [Vibrio cholerae]
           Length = 636
           
 Score =  256 bits (648), Expect = 4e-67
 Identities = 197/644 (30%), Positives = 316/644 (48%), Gaps = 48/644 (7%)

Query: 1   MCGINGF---SWGDEELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPKGH 55
           MCG+ GF        + +  M ++I HRGP+D G++VDD+  V L H RL+I DLS  GH
Sbjct: 1   MCGVAGFISKRLSPVDCLTSMVESIMHRGPNDSGLWVDDDFGVCLAHARLSIQDLSSAGH 60

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTF-TSNTDTEVILAAYLEWGFD-CV 113
           QPM + K  + V +++NGEIYN + +R+EL E   ++   ++DTE +LA +  WG +  +
Sbjct: 61  QPM-HSKSERYV-MIFNGEIYNHLTLREELIEIVPSYWNGHSDTETLLAGFEVWGIEQTI 118

Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
           ++  GM+A V++DK    L L RDRFG KPLYY +     +F+SE+K +  H +     N
Sbjct: 119 QKCVGMFAIVLWDKVLKQLILIRDRFGEKPLYYGWQRDTFLFASELKALKAHPSFEGSIN 178

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
              +  F   N +  T  + +EGI +L PG  AVF    +      ++D    +   +  
Sbjct: 179 RQALSHFFRLNYIP-TPLSIYEGIFKLEPGVIAVFSHEGQLLSKQTFWDASHAV-SLQNF 236

Query: 233 SDPQG---FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVF 289
           SD        DL  ++++ + ++DVP+G+ LSGG+DSS++V  ++  L    +K F++ F
Sbjct: 237 SDHDAVDKLDDLIKQSIQDQALSDVPLGAFLSGGVDSSTVVGILQS-LSTRPVKTFTIGF 295

Query: 290 PGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMK 349
              + +E+     V             + ED LA I  L    +EPF+  S    + V K
Sbjct: 296 DHADFNEASEASDVAKHLGTDHVELIVSAEDALAIINQLPVMYDEPFADASQVPTFLVSK 355

Query: 350 LARENGMKVLLDGQGSDEILAG---YHY---FFGY-------------YFVELLRKFKWG 390
           LA++  + V L G G DE+  G   YHY    + Y              F+  L    W 
Sbjct: 356 LAKKE-VTVCLSGDGGDELFCGYNRYHYTAKVWSYLEKIPFPIRKMLSVFLLTLSPSSWD 414

Query: 391 KLLREI--------LAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFK 442
            L + +        L  K   G+   LK   +  L   V    W    P ++   +EK  
Sbjct: 415 VLSKTLGLNTRLPNLGNKTQKGAQA-LKARDIEDLYTRVVSN-WDLDEPLVKNTAVEKLP 472

Query: 443 KRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLS 502
                  +  +  L + +L  +  Y +  +L   D+  M  S+E RVP  DH + E+  S
Sbjct: 473 FLSDLTELSHLNDLEKMMLWDKQSYLMDDVLVKTDRATMACSLEGRVPLLDHRIAEFAAS 532

Query: 503 LPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDS 562
           LP   K   G  K LLR+ L   +P ++  R  K GF+ P  + L+    + +A  ++DS
Sbjct: 533 LPIHLKYRGGKGKWLLREVLYRYVPKKLIERPKK-GFSLPIAEWLR-GPLKDWANVLLDS 590

Query: 563 ESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLRE 606
           + F K  +     V + + EH++GK++WS +LW V++ +LWL +
Sbjct: 591 DRFDKEGFLSSELVQKKWGEHLAGKRDWSSQLWSVLMFQLWLEK 634


>sp|P54420|ASNB_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 1
           >gi|7474447|pir||H69590 asparagine synthetase asnB -
           Bacillus subtilis >gi|2293165|gb|AAC00243.1| (AF008220)
           asparagine synthase [Bacillus subtilis]
           >gi|2635538|emb|CAB15032.1| (Z99119) asparagine
           synthetase [Bacillus subtilis]
           Length = 632
           
 Score =  256 bits (648), Expect = 4e-67
 Identities = 183/620 (29%), Positives = 310/620 (49%), Gaps = 59/620 (9%)

Query: 12  EELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVY 71
           EEL+K+MN  I HRGPD +G + D++V  G  RL+IID+   G QP+ YE +    WI++
Sbjct: 20  EELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVE-NGGQPLSYEDE--TYWIIF 76

Query: 72  NGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNI 131
           NGEIYN++E+R+ELE KGYTF +++DTEV+LA Y  +  +   +  GM+AF+I++K+ ++
Sbjct: 77  NGEIYNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASKLRGMFAFLIWNKNDHV 136

Query: 132 LFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDT 191
           L+ +RD FGIKPLYY      + F+SE K ++      + +   +  ++ +  +     T
Sbjct: 137 LYGARDPFGIKPLYYTTINDQVYFASERKSLMVAQNDIEIDKEALQQYMSFQFVPE-PST 195

Query: 192 FFEGIKRLMPGYNAVFDLNTRKFEAWK-YYDLRERIEKRKEISDPQGFKDLFLRAVKMRL 250
               +K++ PG  + F +       +K Y+    +  + +E    +  +D    +V + +
Sbjct: 196 LDAHVKKVEPG--SQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKEVRDAIYDSVNVHM 253

Query: 251 VADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVS 310
            +DVPVGS LSGG+DSS IV   +   P   +K FS+ F  +   E    +       + 
Sbjct: 254 RSDVPVGSFLSGGIDSSFIVSVAKEFHP--SLKTFSVGFEQQGFSEVDVAKETAAALGIE 311

Query: 311 WYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILA 370
                 + E+ + ++  ++   ++P +  +    Y V K A+++ + V L G+G+DE+  
Sbjct: 312 NISKVISPEEYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKH-VTVALSGEGADELFG 370

Query: 371 GYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWVT----KRLW 426
           GY+ +                  RE L+ K        LK M+L++  V       K L 
Sbjct: 371 GYNIY------------------REPLSLKPFERIPSGLKKMLLHVAAVMPEGMRGKSLL 412

Query: 427 RRRFPYLREEFI---EKFKKRPSKELIWKIKTLNEALLLAETYYS--------------- 468
            R    L++ +I   + F++   K+L+           + +TY++               
Sbjct: 413 ERGCTPLQDRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVD 472

Query: 469 -----LPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLK 523
                   +L   DK  M  S+E RVPF D  + +    +P E K   G TK LLRK  +
Sbjct: 473 IHTWMRGDILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAE 532

Query: 524 GILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEH 583
           GI+P+ + NR  K+GF  P +  LK NE  ++   +I         + DY  VL++ E+H
Sbjct: 533 GIVPEHVLNR-KKLGFPVPIRHWLK-NEMNEWVRNIIQESQTDAYIHKDY--VLQLLEDH 588

Query: 584 VSGKKNWSQELWKVIIMELW 603
            + K + S+++W V+I  +W
Sbjct: 589 CADKADNSRKIWTVLIFMIW 608


>dbj|BAA33606.1| (AB012956) probable asparagine synthetase [Vibrio cholerae]
           Length = 636
           
 Score =  255 bits (645), Expect = 1e-66
 Identities = 195/643 (30%), Positives = 313/643 (48%), Gaps = 46/643 (7%)

Query: 1   MCGINGF---SWGDEELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPKGH 55
           MCG+ GF        + +  M ++I HRGP+D G++VDD+  V L H RL+I DLS  GH
Sbjct: 1   MCGVAGFISKRLSPVDCLTSMVESIMHRGPNDSGLWVDDDFGVCLAHARLSIQDLSSAGH 60

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTF-TSNTDTEVILAAYLEWGFD-CV 113
           QPM + K  + V +++NGEIYN + +R+EL E   ++   ++DTE +LA +  WG +  +
Sbjct: 61  QPM-HSKSERYV-MIFNGEIYNHLTLREELIEIVPSYWNGHSDTETLLAGFEVWGIEQTI 118

Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
           ++  GM+A V++DK    L L RDRFG KPLYY +     +F+SE+K +  H +     N
Sbjct: 119 QKCVGMFAIVLWDKVLKQLILIRDRFGEKPLYYGWQRDTFLFASELKALKAHPSFEGSIN 178

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
              +  F   N +  T  + +EGI +L PG  AVF    +      ++D    +   +  
Sbjct: 179 RQALSHFFRLNYIP-TPLSIYEGIFKLEPGVIAVFSHEGQLLSKQTFWDASHAV-SLQNF 236

Query: 233 SDPQG---FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVF 289
           SD        DL  ++++ + ++DVP+G+ LSGG+DSS++V  ++  L    +K F++ F
Sbjct: 237 SDHDAVDKLDDLIKQSIQDQALSDVPLGAFLSGGVDSSTVVGILQS-LSTRPVKTFTIGF 295

Query: 290 PGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMK 349
              + +E+     V             + ED LA I  L    +EPF+  S    + V K
Sbjct: 296 DHADFNEASEASDVAKHLGTDHVELIVSAEDALAIINQLPVMYDEPFADASQVPTFLVSK 355

Query: 350 LARENGMKVLLDGQGSDEILAG---YHY---FFGY-------------YFVELLRKFKWG 390
           LA++  + V L G G DE+  G   YHY    + Y              F+  L    W 
Sbjct: 356 LAKKE-VTVCLSGDGGDELFCGYNRYHYTAKVWSYLEKIPFPIRKMLSVFLLTLSPSSWD 414

Query: 391 KLLREILAYKR-------IHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK 443
            L + +    R       I      LK   +  L   V    W    P ++   +EK   
Sbjct: 415 VLSKTLGLNTRLPNLGNKIQKGAQALKARDIEDLYTRVVSN-WDLDEPLVKNTAVEKLPF 473

Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
                 +  +  L + +L  +  Y +  +L   D+  M  S+E RVP  DH + E+  SL
Sbjct: 474 LSDLTELSHLNDLEKMMLWDKQSYLMDDVLVKTDRATMACSLEGRVPLLDHRIAEFAASL 533

Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSE 563
           P   K   G  K LLR+ L   +P ++  R  K GF+ P  + L+    + +A  ++DS+
Sbjct: 534 PIHLKYRGGKGKWLLREVLYRYVPKKLIERPKK-GFSLPIAEWLR-GPLKDWANVLLDSD 591

Query: 564 SFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLRE 606
              K  +     V + + EH++GK++WS +LW V++ +LWL +
Sbjct: 592 RIDKEGFLSSELVQKKWREHLAGKRDWSSQLWSVLMFQLWLEK 634


>emb|CAA69108.1| (Y07786) ORF71x8 [Vibrio cholerae]
           Length = 636
           
 Score =  254 bits (642), Expect = 2e-66
 Identities = 195/643 (30%), Positives = 312/643 (48%), Gaps = 46/643 (7%)

Query: 1   MCGINGF---SWGDEELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPKGH 55
           MCG+ GF        + +  M  +I HRGP+D G++VDD+  V L H RL+I DLS  GH
Sbjct: 1   MCGVAGFISKRLSPVDCLTSMVGSIMHRGPNDSGLWVDDDFGVCLAHARLSIQDLSSAGH 60

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTF-TSNTDTEVILAAYLEWGFD-CV 113
           QPM + K  + V +++NGEIYN + +R+EL E   ++   ++DTE +LA +  WG +  +
Sbjct: 61  QPM-HSKSERYV-MIFNGEIYNHLTLREELIEIVPSYWNGHSDTETLLAGFEVWGIEQTI 118

Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
           ++  GM+A V++DK    L L RDRFG KPLYY +     +F+SE+K +  H +     N
Sbjct: 119 QKCVGMFAIVLWDKVLKQLILIRDRFGEKPLYYGWQRDTFLFASELKALKAHPSFEGSIN 178

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
              +  F   N +  T  + +EGI +L PG  AVF    +      ++D    +   +  
Sbjct: 179 RQALSHFFRLNYIP-TPLSIYEGIFKLEPGVIAVFSHEGQLLSKQTFWDASHAV-SLQNF 236

Query: 233 SDPQG---FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVF 289
           SD        DL  ++++ + ++DVP+G+ LSGG+DSS++V  ++  L    +K F++ F
Sbjct: 237 SDHDAVDKLDDLIKQSIQDQALSDVPLGAFLSGGVDSSTVVGILQS-LSTRPVKTFTIGF 295

Query: 290 PGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMK 349
              + +E+     V             + ED LA I  L    +EPF+  S    + V K
Sbjct: 296 DHADFNEASEASDVAKHLGTDHVELIVSAEDALAIINQLPVMYDEPFADASQVPTFLVSK 355

Query: 350 LARENGMKVLLDGQGSDEILAG---YHY---FFGY-------------YFVELLRKFKWG 390
           LA++  + V L G G DE+  G   YHY    + Y              F+  L    W 
Sbjct: 356 LAKKE-VTVCLSGDGGDELFCGYNRYHYTAKVWSYLEKIPFPIRKMLSVFLLTLSPSSWD 414

Query: 391 KLLREILAYKR-------IHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK 443
            L + +    R       I      LK   +  L   V    W    P ++   +EK   
Sbjct: 415 VLSKTLGLNTRLPNLGNKIQKGAQALKARDIEDLYTRVVSN-WDLDEPLVKNTAVEKLPF 473

Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
                 +  +  L + +L  +  Y +  +L   D+  M  S+E RVP  DH + E+  SL
Sbjct: 474 LSDLTELSHLNDLEKMMLWDKQSYLMDDVLVKTDRATMACSLEGRVPLLDHRIAEFAASL 533

Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSE 563
           P   K   G  K LLR+ L   +P ++  R  K GF+ P  + L+    + +A  ++DS+
Sbjct: 534 PIHLKYRGGKGKWLLREVLYRYVPKKLIERPKK-GFSLPIAEWLR-GPLKDWANVLLDSD 591

Query: 564 SFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLRE 606
              K  +     V + + EH++GK++WS +LW V++ +LWL +
Sbjct: 592 RIDKEGFLSSELVQKKWREHLAGKRDWSSQLWSVLMFQLWLEK 634


>gb|AAF34252.1|AF168003_7 (AF168003) putative asparagine synthetase [Desulfovibrio gigas]
           Length = 643
           
 Score =  250 bits (632), Expect = 3e-65
 Identities = 195/642 (30%), Positives = 302/642 (46%), Gaps = 49/642 (7%)

Query: 1   MCGINGFSWGDE----------ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDL 50
           MCGI GF    +          +++  M   I HRGPD  G +V    +  H RLAIIDL
Sbjct: 1   MCGIAGFVLLQDAPPVTDHQARDVLAAMAGRIAHRGPDGRGHHVGLPAAFAHCRLAIIDL 60

Query: 51  SPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGF 110
           S  G QPM  E DG  V + +NGEIYN+ E+R+EL+  G TF + +DTEV+L AYLEWG 
Sbjct: 61  ST-GAQPMAAE-DGAAV-VTFNGEIYNYQEVRRELQALGRTFQTRSDTEVLLQAYLEWGV 117

Query: 111 DCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRK 170
            C++R  GM+AF ++D  +  LF +RDRFG KP +Y        F+SE+  +    +P  
Sbjct: 118 ACLDRLEGMFAFALWDVRRRTLFAARDRFGKKPFFYTIQQGVFAFASELSAL--EAVPWA 175

Query: 171 P---NDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEA-WKYYDLRERI 226
                 + +  FL Y  +   E  + E  K     Y  + +  T   E  W+    R   
Sbjct: 176 AFSIRASTLARFLAYEYVPTPESIYAEARKLPPAQYLELHEDGTLLVERYWETPMPRPEG 235

Query: 227 EKRKEISDP----QGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI 282
            K    +D     +  + L  +AV+ RL+ADVPVG  LSGG+DSSS+      L P   I
Sbjct: 236 SKGFGRADEPALCEELRRLLTQAVRRRLIADVPVGVFLSGGVDSSSVAAMAGQLAPG--I 293

Query: 283 KVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADII-DLIRTQEEPFSTLSI 341
           K FS+ F     DES Y   V  +     ++      D  AD++ +++   +EP +  SI
Sbjct: 294 KTFSIGFTEASYDESAYAGRVAAQLGTD-HQLELLSADACADLLPEVVSRFDEPMADPSI 352

Query: 342 YGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKR 401
              Y + ++ R   + V L G G DE+LAGY Y+ G+   +   +         + A   
Sbjct: 353 VPTYLLSQITRRQ-VTVALGGDGPDELLAGYEYYPGFKATQYWERLPAWLRRAAVAASGL 411

Query: 402 IHGSL-------VPLKNMVLYILPVWV-TKRLWRRRFPYLRE-------EFIEKFKKRPS 446
           + GS        V    +     P W+ T+R+     P  +        + +E  +   S
Sbjct: 412 LPGSAGYVNPRHVAQTFLAAAEAPAWLRTQRMLTALTPEAQRALWKSPGDMLEPTRLFAS 471

Query: 447 KELIWKI----KTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLS 502
            + +W+       +     L     +  ++L   D+  M   +E+R PF D +L E+   
Sbjct: 472 TKRLWEALPDWPPMARVFHLFAQQITYGYILVKVDRCTMLHGLEARAPFLDRDLAEFCFR 531

Query: 503 LPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDS 562
           LP   K+     K LL++ + G+LP +I +R  K GF  P    L+    +     ++  
Sbjct: 532 LPVSMKLRGATRKYLLKEAMAGLLPQDILHR-PKRGFLIPTAAWLR-GRLRPLVDDLLHE 589

Query: 563 ESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWL 604
              +++  +    V R+ EEH SGKK+  + LW +++++LWL
Sbjct: 590 RRLREQGLFHPAAVRRLVEEHASGKKDHRKGLWTMLVLQLWL 631


>dbj|BAB05227.1| (AP001512) asparagine synthetase [Bacillus halodurans]
           Length = 615
           
 Score =  242 bits (612), Expect = 7e-63
 Identities = 171/560 (30%), Positives = 274/560 (48%), Gaps = 58/560 (10%)

Query: 1   MCGINGF-SW-----GDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI G+  W      + E +K+M +   HRGPDD  V+ + + +LGH RL ++D  P+G
Sbjct: 1   MCGITGWVDWRRNLQNETETIKQMAETQTHRGPDDLNVWTEKHAALGHSRLIVVD--PEG 58

Query: 55  H-QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
             QPM  E++GK   IVYNGE+YN  ++RKEL  KGY F  ++DTEV+L +Y+EWG+ CV
Sbjct: 59  GCQPMMRERNGKRYTIVYNGELYNTEDLRKELIVKGYQFQGHSDTEVLLVSYIEWGYQCV 118

Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPN 172
           E+FNG++AF I++     LF++RDR G+KPL+Y      ++F++EIK +L H  I     
Sbjct: 119 EKFNGIFAFAIWNDKDQSLFMARDRLGVKPLFYTVRHGFLLFATEIKALLAHPEIEPVLT 178

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           +  + + L         +  F+ I+ L P +   +D N  K    +Y+ L+        +
Sbjct: 179 EEGLSEVLGLGPSRSPGNGVFDDIQELRPAHLLTYDRNGAKVS--RYWRLKSMAHSEDAM 236

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGK 292
                 +DL    V+ +L ADVPVG  LSGG+DSS++  A+  L+ + E K     +   
Sbjct: 237 ETAAHLRDLLEDTVERQLFADVPVGMFLSGGVDSSALT-AIAALIYEREGKGLIRTYSID 295

Query: 293 EIDESKYQRI--------------VVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFST 338
             +  KY +               V    R + +    ++E++   +   +  ++ P   
Sbjct: 296 YDENDKYFKANDFQPNADGPWVEKVSTTFRTNHHNAVISIEELANQLKRAVELRDLPGMA 355

Query: 339 LSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLL--REI 396
                 Y   K  +++ + V L G+ +DEI  GY +F     +     F W +    R+ 
Sbjct: 356 DVDSSLYWFCKQIKKD-VTVGLSGECADEIFGGYPWFHKPEVMN-FNGFPWMRSADERQE 413

Query: 397 LAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRF---PYLREEFIEKFKKRPSKELIWKI 453
           L ++R                        WR++     Y+ + + E   + P  E     
Sbjct: 414 LLHER------------------------WRQKLNLPKYVHDRYKETIAETPRFEEDTPE 449

Query: 454 KTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGI 513
           +     +      + +  LL  +D+ +M  S+E RVPF DH +VEY  ++P E K   G 
Sbjct: 450 EARRREISYLNMVWFMTTLLDRKDRMSMGASLEVRVPFSDHRIVEYAWNIPWEIKQFGGR 509

Query: 514 TKVLLRKGLKGILPDEIRNR 533
            K +LRK L+GILPDE+  R
Sbjct: 510 EKGILRKALEGILPDEVLYR 529


>sp|O05272|ASNO_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 3
           >gi|1934835|emb|CAB07965.1| (Z93940) asparagine
           synthetase [Bacillus subtilis]
           Length = 614
           
 Score =  240 bits (607), Expect = 3e-62
 Identities = 169/558 (30%), Positives = 275/558 (48%), Gaps = 56/558 (10%)

Query: 1   MCGINGFSWGDEELVK------RMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI G+    ++LV+      RM D +  RGPDD  V+ + +V  GH RLA++D+   G
Sbjct: 1   MCGITGWVDFKKQLVQEKQTMDRMTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEG-G 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QPM     G    I+YNGE+YN  ++RKEL  +G+ F   +DTEV+L +Y+EW  DCV+
Sbjct: 60  RQPMACTYKGDTYTIIYNGELYNTEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVD 119

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDA 174
             NG++AF ++D+ +N+LF +RDR G+KP +Y  +G + +F SEIK IL H   +   D 
Sbjct: 120 HLNGIFAFAVWDEKRNLLFAARDRLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDR 179

Query: 175 VIFDFLYYNLLDHTEDT-FFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEIS 233
                ++      T  T  F+GIK + P +   F  +      W+Y+++    EK  +  
Sbjct: 180 TGLSEIFGLGPSRTPGTGIFKGIKEIRPAHALTFSKD--GLNIWRYWNVES--EKHTDSF 235

Query: 234 DP--QGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVFP 290
           D      + LF  AV  +LV+DVPV + LSGGLDSS+I   A  H   +G+  + +    
Sbjct: 236 DDTVANVRSLFQDAVTRQLVSDVPVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSID 295

Query: 291 GKEIDE----SKYQ--------RIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFST 338
            +E D+    S +Q          + +    + ++   + +D++  + + +  ++ P   
Sbjct: 296 YEENDKYFQASAFQPNDDGPWIEKMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLP-GM 354

Query: 339 LSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILA 398
             +           +    V L G+ +DEI  GY +F   +  ++   F W +   E + 
Sbjct: 355 ADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWF---HTADVESGFPWMRSTEERI- 410

Query: 399 YKRIHGSLVPLKNMVLYILPVWVTKRLWRRRF---PYLREEFIEKFKKRPSKELIWKIKT 455
            K +  S                    W+++     Y+  ++ E   + P  +    +  
Sbjct: 411 -KLLSDS--------------------WQKKLNLKEYVNAKYEETLAETPLLDGETGVDK 449

Query: 456 LNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITK 515
               L      + + +LL  +D+ +M  S+E RVPF DH LVEYV ++P E K+     K
Sbjct: 450 ARRQLFYLNMLWFMTNLLDRKDRMSMGASLEVRVPFADHRLVEYVWNIPWEMKMHDNREK 509

Query: 516 VLLRKGLKGILPDEIRNR 533
            +LRK L+GILPD+I  R
Sbjct: 510 GILRKALEGILPDDILYR 527


>pir||F69837 asparagine synthase homolog yisO - Bacillus subtilis
           >gi|2145380|emb|CAA70643.1| (Y09476) YisO [Bacillus
           subtilis] >gi|2633415|emb|CAB12919.1| (Z99109) alternate
           gene name: yucB~similar to asparagine synthase [Bacillus
           subtilis]
           Length = 591
           
 Score =  232 bits (586), Expect = 8e-60
 Identities = 161/535 (30%), Positives = 263/535 (49%), Gaps = 50/535 (9%)

Query: 18  MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYN 77
           M D +  RGPDD  V+ + +V  GH RLA++D+   G QPM     G    I+YNGE+YN
Sbjct: 1   MTDTLSKRGPDDSNVWGEHHVLFGHKRLAVVDIEG-GRQPMACTYKGDTYTIIYNGELYN 59

Query: 78  FMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRD 137
             ++RKEL  +G+ F   +DTEV+L +Y+EW  DCV+  NG++AF ++D+ +N+LF +RD
Sbjct: 60  TEDLRKELRARGHQFERTSDTEVLLHSYIEWQEDCVDHLNGIFAFAVWDEKRNLLFAARD 119

Query: 138 RFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDT-FFEGI 196
           R G+KP +Y  +G + +F SEIK IL H   +   D      ++      T  T  F+GI
Sbjct: 120 RLGVKPFFYTKEGSSFLFGSEIKAILAHPDIKARVDRTGLSEIFGLGPSRTPGTGIFKGI 179

Query: 197 KRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDP--QGFKDLFLRAVKMRLVADV 254
           K + P +   F  +      W+Y+++    EK  +  D      + LF  AV  +LV+DV
Sbjct: 180 KEIRPAHALTFSKD--GLNIWRYWNVES--EKHTDSFDDTVANVRSLFQDAVTRQLVSDV 235

Query: 255 PVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVFPGKEIDE----SKYQ--------R 301
           PV + LSGGLDSS+I   A  H   +G+  + +     +E D+    S +Q         
Sbjct: 236 PVCTFLSGGLDSSAITAIAAGHFEKEGKAPLHTYSIDYEENDKYFQASAFQPNDDGPWIE 295

Query: 302 IVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLD 361
            + +    + ++   + +D++  + + +  ++ P     +           +    V L 
Sbjct: 296 KMTEAFGTTHHKCVISQKDLVDHLEEAVLVKDLP-GMADVDSSLLWFCREIKKDFVVSLS 354

Query: 362 GQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWV 421
           G+ +DEI  GY +F   +  ++   F W +   E +  K +  S                
Sbjct: 355 GECADEIFGGYPWF---HTADVESGFPWMRSTEERI--KLLSDS---------------- 393

Query: 422 TKRLWRRRF---PYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDK 478
               W+++     Y+  ++ E   + P  +    +      L      + + +LL  +D+
Sbjct: 394 ----WQKKLNLKEYVNAKYEETLAETPLLDGETGVDKARRQLFYLNMLWFMTNLLDRKDR 449

Query: 479 NAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNR 533
            +M  S+E RVPF DH LVEYV ++P E K+     K +LRK L+GILPD+I  R
Sbjct: 450 MSMGASLEVRVPFADHRLVEYVWNIPWEMKMHDNREKGILRKALEGILPDDILYR 504


>pir||F83384 probable asparagine synthetase PA2084 [imported] - Pseudomonas
           aeruginosa (strain PAO1)
           >gi|9948096|gb|AAG05472.1|AE004636_3 (AE004636) probable
           asparagine synthetase [Pseudomonas aeruginosa]
           Length = 610
           
 Score =  228 bits (575), Expect = 2e-58
 Identities = 187/567 (32%), Positives = 277/567 (47%), Gaps = 54/567 (9%)

Query: 1   MCGINGFS-WGDE-----ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI G++ W  +      +++RM   +  RGPD EG+++  +  LGH RLAIIDL   G
Sbjct: 1   MCGITGWTDWSRDLGEQRSILERMTRTLAPRGPDAEGLWLSRHALLGHRRLAIIDLE-NG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QPM  E  G+ V + Y+GE+YNF E+R ELE  G  F + +DTEV+L AYL+WG     
Sbjct: 60  AQPMLAEAGGR-VALTYSGEVYNFRELRAELETLGQVFRTRSDTEVVLRAYLQWGEASFV 118

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDA 174
           R  G++ F ++D+ +  L+L RDRFG+KPLYYY     ++F+SE K I  +       DA
Sbjct: 119 RLRGIYGFALWDEREQSLWLVRDRFGVKPLYYYPTAGGVLFASEPKAIFANPEANPVLDA 178

Query: 175 V-IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLN-TRKFEAWKYYDLRERIEKRKEI 232
             I +              ++G++++ PG    FD N  R+   W       R E+ +E 
Sbjct: 179 SGIAELFALTTAPTPGHGLYKGLRQVRPGQALRFDRNGVRELVYWAL-----RAERHEEG 233

Query: 233 SDPQGFK--DLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE---IKVFSL 287
           ++    +   L   AV  +LVADVP+GS LSGGLDSS+I  A      DG+   +  FS+
Sbjct: 234 AEDSAERAGQLLREAVAEQLVADVPLGSLLSGGLDSSAI-SAFAVAALDGDARRLPTFSV 292

Query: 288 VFPG----------KEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFS 337
            FPG          +   +  Y ++  +             E+VL     +++ ++ P  
Sbjct: 293 DFPGEGRGFVPTPWQSSWDEPYAQLAANFLGTPHRTVLVAPEEVLEQDEAVLQARDLPGW 352

Query: 338 TLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREIL 397
                  Y + +  RE+   V L G+ +DE+  GY +F     +     F W       L
Sbjct: 353 GELDASLYLLFRQVREH-TTVALSGESADEVFGGYPFFHDPSAL-AHDGFPW-------L 403

Query: 398 AYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLN 457
           A K     L+  +       P ++  R +R     +     E   +R  +E+ +      
Sbjct: 404 AGKSGPWQLLRREVAERVAAPEYIRAR-YREALAEVPRLDGEDAAQRRQREVAY------ 456

Query: 458 EALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVL 517
             L L+     LP +L  +D+ +M   +E RVPFCDH LVEYV +LP   K  AG +K L
Sbjct: 457 --LALSRW---LPAMLERKDRMSMAVGLEVRVPFCDHRLVEYVWNLPWALKSVAGESKSL 511

Query: 518 LRKGLKGILPDEIRNRASKVGF-ATPD 543
           LR+ L+G LP  I  R  K GF A PD
Sbjct: 512 LRRALRGHLPQAILER-RKSGFPANPD 537


>gb|AAF24002.1| (AF035937) WbpS [Pseudomonas aeruginosa]
           Length = 627
           
 Score =  226 bits (569), Expect = 8e-58
 Identities = 186/643 (28%), Positives = 308/643 (46%), Gaps = 57/643 (8%)

Query: 1   MCGINGFSW-------GDEELVKR-MNDAIRHRGPDDEGVYVDDNVS--LGHVRLAIIDL 50
           MCGI GF W       GD   V R M  AI HRGPD+ G++ +   +  L H RLA+++L
Sbjct: 1   MCGIAGF-WNITGTLLGDNARVARQMAAAIHHRGPDESGIWYEAPRAPILVHARLAVLEL 59

Query: 51  SPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFT--SNTDTEVILAAYLEW 108
           SP G QPM    D     ++YNGEIYN + +R  L E G T +    +DTE +LA + +W
Sbjct: 60  SPAGSQPM--HSDCGRYVLIYNGEIYNHLALRARLSEAGVTHSWRGGSDTETLLACFAQW 117

Query: 109 GFDCVERFN-GMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNI 167
           G +   +   GM+A  ++D+ +  + L+RDR G KPLY+ +    + F+SE+K + +H +
Sbjct: 118 GVESTLKLTVGMFALALWDRQEKTITLARDRMGEKPLYWGWQNGVLFFASELKALKEHPL 177

Query: 168 PRKPNDA-VIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNT--RKFEAWKYYDLRE 224
            R   D   +  FL Y  +     + ++GI +L  G   V    +     E   Y+    
Sbjct: 178 FRGDIDRDALALFLRYGYVP-APYSIYKGIGKLRAGSYLVLSERSLNETCEPAAYWSANA 236

Query: 225 RIEKRKEISDP-QG--------FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRH 275
            IE  + +S+P QG         +     ++  ++V+DVP+G+ LSGG+DSS++V  M+ 
Sbjct: 237 AIE--EALSNPFQGTDAEAVDLLESQLRTSISDQMVSDVPLGAFLSGGVDSSTVVALMQQ 294

Query: 276 LLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEP 335
                 I+ FS+ F     DE+ Y + V +           N +D L  I  L +   EP
Sbjct: 295 -QSSRPIRTFSIGFDEPGYDEAVYAKAVAEHIGTDHTELYVNSKDALDVIPSLPKIYCEP 353

Query: 336 FSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYH-YFFGYYFVELLRKFKWGKLLR 394
           F   S      V  LAR+  + V L G G DE+  GY+ Y F      +L +F       
Sbjct: 354 FGDSSQIPTLIVSGLARQQ-VTVALSGDGGDELFGGYNPYQFTPRVWRMLERFP-----H 407

Query: 395 EILAYKRIHGSLVPLKNMVLYILPVWVTKRL----------WR-RRFPYLREEFIEKFKK 443
            +  +       +PL   +  +  V+ ++            WR   +P +  +       
Sbjct: 408 SMRRFASAFAQDLPLPEKLGKLRDVFASRTAEELFYRLNSHWRNHEYPVIGAQGHTALLD 467

Query: 444 RPSKELIW-KIKTLNEALLLAETYYSLP-HLLRFEDKNAMRWSIESRVPFCDHELVEYVL 501
            P +   W ++ +    ++  +    +P  +L   D+ AM  S+E+RVP  DH + E   
Sbjct: 468 TPER---WPRVDSFQHWMMAMDVQGYMPDDILVKVDRAAMANSLETRVPLIDHRVFELAW 524

Query: 502 SLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVID 561
            +P   K+  G  K LLR+ L   +  E+  R  K GF+ P  D L+    +++A +++D
Sbjct: 525 RMPLHMKIRNGKGKWLLREVLYRHVSRELIERPKK-GFSVPVSDWLR-GPLKEWAESLLD 582

Query: 562 SESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWL 604
               ++  Y D R + R++ +H++G+++ S+ LW V++ + WL
Sbjct: 583 ERRLQQEGYLDSRLIRRIWNDHLAGRRDHSRRLWSVLMFQAWL 625


>emb|CAB58327.1| (AL121855) putative amidotransferase [Streptomyces coelicolor
           A3(2)]
           Length = 644
           
 Score =  220 bits (556), Expect = 3e-56
 Identities = 184/662 (27%), Positives = 305/662 (45%), Gaps = 89/662 (13%)

Query: 1   MCGING-FSWGDEELVK-RMNDAIRHRGPDDEGVYV----DDNVSLGHVRLAIIDLSPKG 54
           MCGI G + W D + V  R+ D + HRGPD  G Y     +  V LGH RLA++DLS  G
Sbjct: 1   MCGIAGTYHWPDGKAVTDRLTDVLAHRGPDGAGRYSHPAGEGEVHLGHRRLAVVDLSTTG 60

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QPM  E     + + YNGE+YN  E+R ELE  G  F   +DTEV+L A+  WG DC+ 
Sbjct: 61  AQPMVSEG----LALSYNGELYNAPELRTELESAGVRFRGTSDTEVVLEAWRRWGTDCLP 116

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND- 173
           +  GM+AF ++D+    L L+RD+ GIKPL+    G  ++F+SE+K +      +   D 
Sbjct: 117 QLRGMFAFAVFDERTGELVLARDQLGIKPLFLLRRGAGLVFASELKALATATGGKLEVDH 176

Query: 174 -AVIFDFLYYNLLDHTEDTFFEGIKRLMPG--YNAVFDLNTRKFEAWKYYDLRERIEKRK 230
            A++   LYY + D          ++L PG       D    +   W   D+     +R 
Sbjct: 177 AALVASLLYYWVPD--SRCALREAEKLPPGSWLRCRPDGRVERGRFWNLRDVAAEGRERA 234

Query: 231 EISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFP 290
              +      +   + +  L++DVPV + LSGGLDSS +        P   I  +++ F 
Sbjct: 235 RSGERPDLAAVVEESTRRHLLSDVPVATFLSGGLDSSYLTALAARDRPG--ISAYTIGFR 292

Query: 291 GKEI------DESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQ----EEPFSTLS 340
            ++       D+ +Y R V +   V  +       ++  +++DL+       +EP    +
Sbjct: 293 AEDARFEAMPDDLRYARQVAERFGVDLHEI-----EIAPNVLDLLPRMTYHLDEPIGDPA 347

Query: 341 IYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILA-- 398
               + +   ARE G+KV+L G G+DE+ AGY                  K L  +LA  
Sbjct: 348 AINTFLICSAAREAGVKVMLSGMGADELFAGYR-----------------KHLANVLALR 390

Query: 399 YKRIHGSLVPLKNMVLYILPVWVTKRLWR-----RRF-----------------PYLREE 436
           Y+R+   L    +  +  LPV  ++R +R     +RF                  Y R+E
Sbjct: 391 YQRVPRPLRRGVSAAVGRLPVASSRRGYRSVRFAKRFLSFADLPQETAFRRSYTMYDRQE 450

Query: 437 FIEKFKKRPSKEL---------IWKIKTLNE---ALLLAETYYSLPHL-LRFEDKNAMRW 483
            ++      +  +         +++   L++    + LA+    LP L L + D+++M  
Sbjct: 451 LLDLIDPDLAGTVEDVLTEHADVYEDNDLDDFVNRMCLADARMFLPGLNLAYTDRSSMAA 510

Query: 484 SIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPD 543
           S E RVP+ D E+V+   ++P + K+     K +L++    +LP EI  R   + F+ P 
Sbjct: 511 STEVRVPYVDVEVVKAAFAVPGDRKIVGRQGKAVLKEAATSVLPREIVYRPKGL-FSAPL 569

Query: 544 KDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELW 603
           +  +  +        V D E   +  +     + R+  E  +G++++S+ LW V+ +E W
Sbjct: 570 RAWMSRDLAPLVREVVNDGE-LVRSGFLRRDALRRLVAEDAAGQRDFSKHLWHVLTLEYW 628

Query: 604 LR 605
            R
Sbjct: 629 YR 630


>pir||A83638 probable glutamine amidotransferase PA0051 [imported] - Pseudomonas
           aeruginosa (strain PAO1)
           >gi|9945874|gb|AAG03441.1|AE004445_2 (AE004445) probable
           glutamine amidotransferase [Pseudomonas aeruginosa]
           Length = 610
           
 Score =  210 bits (529), Expect = 4e-53
 Identities = 177/556 (31%), Positives = 269/556 (47%), Gaps = 47/556 (8%)

Query: 1   MCGINGFSWGDEEL------VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCG+ G+     +L      +  M D +  RGPD EG++   N  LGH RLA+IDLS  G
Sbjct: 1   MCGLAGWVDYTRKLDDEFPAIFAMTDTLALRGPDAEGIWKHRNALLGHRRLAVIDLSG-G 59

Query: 55  HQPMKYE-KDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
            QPM Y    G+EV +VY GE+YN   +R+ L   G+ F + +DTEV+L AYL+WG  C 
Sbjct: 60  VQPMSYRFPTGQEVTLVYTGEVYNHDALRERLRRAGHEFRTRSDTEVVLHAYLQWGERCC 119

Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND 173
           E   GM+AF ++D     L L RDR GIKPLYY    + ++F SEIK IL H       D
Sbjct: 120 EYLTGMFAFAVFDGRDGHLLLVRDRLGIKPLYYARHREGLLFGSEIKSILAHPEFAARLD 179

Query: 174 AV-IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           AV + D L   L   T  T F  ++ L+PG+   +  N++  +  +Y+++R +       
Sbjct: 180 AVGLVDLL--TLSRGTSQTPFREVQELLPGHLLSWRPNSQA-KLRRYWEVRRQEHADDLQ 236

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLP---DGEIKVFSLVF 289
           S  Q  ++L  RA+  +L ADVPV S LSGGLDS+++    + +      G+I  FS+ F
Sbjct: 237 STVQRTRELVTRALGAQLHADVPVCSLLSGGLDSTALTGIAQRIAKAEHGGDINSFSVDF 296

Query: 290 PGK---------EIDESKYQRIVVDECRVSWYRTTF--NVEDVLADI-IDLIRTQEEPFS 337
            G+           D+ +   ++  +   S +RT    N E V      ++ R ++ PF+
Sbjct: 297 VGQAEQFRSDDLRPDQDQPFALLAAQYIGSRHRTVLIDNAELVCERAREEVFRAKDVPFT 356

Query: 338 TLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREIL 397
              +     +M         V + G+G+DE+  GY +F     V   R F W   +R  L
Sbjct: 357 FGDMDTSLHLMFGEIRRHSTVAISGEGADELFGGYGWFRDPQAVAAAR-FPWASRVR--L 413

Query: 398 AYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLN 457
               I        +++ Y    +       R+  +L  +  E+ + R    L      L 
Sbjct: 414 PAGFIDAGFNRRCDLLQYQQASYDD---GLRQVEHLAGDSPEERRMREFSHL-----HLK 465

Query: 458 EALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVL 517
             ++L         LL  +D+ +M   +E RVP+ DHELVEYV ++P   K   G  K L
Sbjct: 466 RWMVL---------LLERKDRLSMCNGLEVRVPYTDHELVEYVYNVPWSIKSRDGEEKWL 516

Query: 518 LRKGLKGILPDEIRNR 533
           L++     +P+ +  R
Sbjct: 517 LKRACADYVPEAVLKR 532


>pir||B83214 probable glutamine amidotransferase PA3459 [imported] - Pseudomonas
           aeruginosa (strain PAO1)
           >gi|9949601|gb|AAG06847.1|AE004766_8 (AE004766) probable
           glutamine amidotransferase [Pseudomonas aeruginosa]
           Length = 589
           
 Score =  202 bits (509), Expect = 9e-51
 Identities = 165/547 (30%), Positives = 254/547 (46%), Gaps = 46/547 (8%)

Query: 1   MCGINGFSWGDEEL-----VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI+G    D +      V+R+   +  RGPD  G +    ++LGH RL I+DL+    
Sbjct: 1   MCGISGEFRFDNQAADLAAVERITHHLAPRGPDAWGFHAQGPIALGHRRLKIMDLAEASG 60

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVER 115
           QPM     G  + +V+NG IYN+ E+R ELE  GY F S  DTEV+L  Y  WG D + R
Sbjct: 61  QPMIDPDLG--LAMVFNGAIYNYPELRGELESLGYRFFSGGDTEVLLKGYHAWGADLLPR 118

Query: 116 FNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAV 175
            NGM+AF ++++ +  LFL+RDR GIKPLYY  D   + F+S +  +L+        D  
Sbjct: 119 LNGMFAFAVWERDRQRLFLARDRLGIKPLYYSLDRSRLRFASSLPALLKGGDIASDLDPQ 178

Query: 176 IFDF-LYYNLLDHTEDTFFEGIKRLMPGYNAVFDLN-TRKFEAWKYYDLRERIEKRKEIS 233
             +F L ++ +     T  EG+K+L P      DL+ + +   W   D   R ++R+   
Sbjct: 179 ALNFYLNFHAVVPAPHTLLEGVKKLPPATWMSVDLDGSCEQRTWWTLDYGPRPDERELTL 238

Query: 234 D--PQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFP- 290
           D   +   D    AV +R  A   VG  LSGG+DSS +V  + H      +  FS+ F  
Sbjct: 239 DDWQERVLDGLREAVAIRQRAAREVGVLLSGGVDSSLLV-GLLHEAGVDNLLTFSIGFED 297

Query: 291 --GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVM 348
             G+  DE +Y  ++ +      +R      +V+  +    R   EP  +      Y   
Sbjct: 298 AGGERGDEFQYSDLIAERYHTRHHRLRIGEHEVIEQLPAAFRAMSEPMVSHDCIAFY--- 354

Query: 349 KLARE--NGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSL 406
            LARE     KV+  GQG+DE+ AGYH++            K         AY+      
Sbjct: 355 LLAREVSKHCKVVQSGQGADELFAGYHWY-----------PKVDGADDAFAAYRAAFFD- 402

Query: 407 VPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETY 466
              ++   Y+  V    R+      ++R+ F       P  E       +++AL L  T 
Sbjct: 403 ---RDHEEYLATVGERFRVEDVAGRFVRDHFAS-----PGAE-----AAVDKALRLDSTI 449

Query: 467 YSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGIL 526
             +   ++  D   M W +E+RVPF D+ L E    +P   K+  G  K +L+   + ++
Sbjct: 450 MLVDDPVKRVDNMTMAWGLEARVPFLDYRLAELSARIPARFKLGDG-GKQVLKGAARKVI 508

Query: 527 PDEIRNR 533
           P E+ +R
Sbjct: 509 PSEVIDR 515


>dbj|BAB12569.1| (AB039379) asparagine synthase homolog [Streptomyces aureofaciens]
           Length = 609
           
 Score =  201 bits (505), Expect = 3e-50
 Identities = 175/580 (30%), Positives = 256/580 (43%), Gaps = 78/580 (13%)

Query: 1   MCGINGFSWGD------EELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI G    D       +L++ M D +  RGPD EG++      LGH RL++ID    G
Sbjct: 1   MCGIVGVVDYDAPAEHRRDLLQAMTDTMACRGPDAEGLWFGRAAGLGHRRLSVID-PEHG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QPM  E +G+   I ++GEIYNF E+R EL   G+ F ++ DTEV+L  YL+WG   VE
Sbjct: 60  RQPMTAEHEGRGAAITFSGEIYNFRELRAELTSHGHRFRTSCDTEVVLRGYLQWGAALVE 119

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGIL-QHNIPRKPND 173
           R NGM+AF ++D+    L L RDR G+KPLYY+     + F SE K +L    + R+   
Sbjct: 120 RLNGMFAFAVWDEHSEELLLVRDRMGVKPLYYWPTAAGVRFGSEPKAVLADRTLSRRVGP 179

Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNA-VFDLNTRKFEAWKYYDLRERIEKRKEI 232
             + + L  +++   E   F G+  + PG    V     R+   W       R+E R+  
Sbjct: 180 DGLCEVL--DMVKTPEAAVFSGLVEVRPGQLVRVGRGGVRRQTYW-------RLEAREHT 230

Query: 233 SDPQ----GFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLV 288
            D        +DL    V  +LV+DVP+G+ LSGGLDSS++          G ++ FS+ 
Sbjct: 231 DDLPTTIGTVRDLLADTVTRQLVSDVPLGTLLSGGLDSSAVTALAAR---SGPVRSFSVD 287

Query: 289 FPG-----------KEIDESKYQRIV--VDECRVSWYRTTFNVEDVLADIIDLIRTQEEP 335
           F G           +E D    + +   V    +     +  + D       L  T   P
Sbjct: 288 FRGAADAFASDAVRREADAPYVRELAGHVGADHLEVLLDSPGLSDPAVRSAVLRATDLPP 347

Query: 336 FSTLSIY-GQYRVMKLARENGMKVLLDGQGSDEILAGYHYF----------FGYYFVELL 384
                ++   Y   +  REN + V L G+ +DE+  GY +F          F +      
Sbjct: 348 AYWGDMWPSLYLFFRAVREN-VTVALSGEAADELFGGYRWFRNPLALRAATFPWLTPGSA 406

Query: 385 RKFKWGKLLREILAYK-RIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKK 443
           R F    L +  L  K  + G         L  +PV   +    RR   +    + +F  
Sbjct: 407 RYFGGSSLFQAGLLDKLDLDGYRAARYAEALAEVPVLPGESAPERRMREIGHLNLTRF-- 464

Query: 444 RPSKELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSL 503
                    ++TL                L  +D+ +M   +E RVPFCDH LVEYV ++
Sbjct: 465 ---------VQTL----------------LDRKDRMSMAVGLEVRVPFCDHRLVEYVFNV 499

Query: 504 PPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPD 543
           P E K   G  K LLR  +  +LP  +  R      AT D
Sbjct: 500 PWEMKSFDGREKSLLRAAVADLLPRSVVERVKTPYPATQD 539


>dbj|BAA89484.1| (AB029550) LtsA [Corynebacterium glutamicum]
           Length = 640
           
 Score =  196 bits (494), Expect = 5e-49
 Identities = 177/649 (27%), Positives = 293/649 (44%), Gaps = 63/649 (9%)

Query: 1   MCGI------NGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCG+      NG +      ++R    +RHRGPDD G + D + + G  RL+IID++   
Sbjct: 1   MCGLLGILTANGNAEAFVPALERALPCMRHRGPDDAGTWHDADAAFGFNRLSIIDIA-HS 59

Query: 55  HQPMKY--EKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDC 112
           HQP+++    +     + +NGEIYN++E+RKEL + GYTF ++ D E I+  +  WG   
Sbjct: 60  HQPLRWGPADEPDRYAMTFNGEIYNYVELRKELSDLGYTFNTSGDGEPIVVGFHHWGESV 119

Query: 113 VERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGIL----QHNIP 168
           VE   GM+   I+D  +  LFL+RD+FGIKPL+Y       +FSSE K IL    + N+ 
Sbjct: 120 VEHLRGMFGIAIWDTKEKSLFLARDQFGIKPLFYATTEHGTVFSSEKKTILEMAEEMNLD 179

Query: 169 RKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLR---ER 225
              +   I  ++    +    DT    I RL  G  A       K E  +Y+  +   ++
Sbjct: 180 LGLDKRTIEHYVDLQYVPE-PDTLHAQISRLESGCTATVRPG-GKLEQKRYFKPQFPVQK 237

Query: 226 IEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVF 285
           + K KE         +   +V+  + ADV VGS LSGG+DS++I    +   PD  +  F
Sbjct: 238 VVKGKEQDLFDRIAQVLEDSVEKHMRADVTVGSFLSGGIDSTAIAPLAKRHNPD--LLTF 295

Query: 286 SLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQY 345
           +  F  +   E                    + E+    I  ++   ++P +  S+   Y
Sbjct: 296 TTGFEREGYSEVDVAAESAAAIGAEHIVKIVSPEEYANAIPKIMWYLDDPVADPSLVPLY 355

Query: 346 RVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGS 405
            V   AR++ +KV+L G+G+DE+  GY  +               K    +  +++I   
Sbjct: 356 FVAAEARKH-VKVVLSGEGADELFGGYTIY---------------KEPLSLAPFEKIPSP 399

Query: 406 LVPLKNMVLYILPVWVT-KRLWRRRFPYLREEFIEKFK----KRPSKELIWKIKTLNEAL 460
           L      +  +LP  +  K L  R    + E +    +    ++  + + W  +  +   
Sbjct: 400 LRKGLGKLSKVLPDGMKGKSLLERGSMTMEERYYGNARSFNFEQMQRVIPWAKREWDHRE 459

Query: 461 LLAETYYS---------LPHLLRF----------EDKNAMRWSIESRVPFCDHELVEYVL 501
           + A  Y           + HL  F           DK  M  S+E RVPF D E+ +   
Sbjct: 460 VTAPIYAQSRNFDPVARMQHLDLFTWMRGDILVKADKINMANSLELRVPFLDKEVFKVAE 519

Query: 502 SLPPESKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVID 561
           ++P + K++ G TK  LR+ L+ I+P  + +R  K+GF  P +  L  +E   F  A   
Sbjct: 520 TIPYDLKIANGTTKYALRRALEQIVPPHVLHR-KKLGFPVPMRHWLAGDE--LFGWAQDT 576

Query: 562 SESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELWKVIIMELWLREWMDN 610
            +       ++ + VL M  EH  G  + S+ LW V+   +W   +++N
Sbjct: 577 IKESGTEDIFNKQAVLDMLNEHRDGVSDHSRRLWTVLSFMVWHGIFVEN 625


>gb|AAF17502.1|AF195615_10 (AF195615) PhzH [Pseudomonas chlororaphis]
           Length = 610
           
 Score =  192 bits (484), Expect = 7e-48
 Identities = 159/575 (27%), Positives = 255/575 (43%), Gaps = 85/575 (14%)

Query: 1   MCGINGFS------WGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCG+ G+        G++  ++ M + +  RGPD EG++   +  LGH RLA+IDLS  G
Sbjct: 1   MCGLTGWVDYTRRLEGEDPAIRAMTNTLALRGPDAEGIWKHRHALLGHRRLAVIDLSG-G 59

Query: 55  HQPMKYE-KDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
            QPM Y   DG+EV +VY GE+YN   +R +L + G+ F + +DTEV+L AYLEWG  C 
Sbjct: 60  TQPMVYRFADGQEVSLVYTGEVYNHDALRDQLRQAGHVFQTRSDTEVVLHAYLEWGERCC 119

Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND 173
           +   GM+AF ++D     L L RDR GIKPL+Y    + ++F SE K IL H  P     
Sbjct: 120 DHLTGMFAFALFDGRDGHLLLVRDRLGIKPLFYARHREGLLFGSETKAILAH--PEFTTG 177

Query: 174 AVIFDFL-YYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
             +   +    L   T  T F  +  L+PG+   +  N  + +   Y+ +R +  +    
Sbjct: 178 LEVTGLVDVLTLSKGTAQTPFRNLMELLPGHFLSWRPN-GQMKIQSYWKVRRQEHQHDLQ 236

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL---PDGEIKVFSLVF 289
           +  Q  ++L   A+  +L ADVPV S LSGGLDS+++    + ++     G I  FS+ F
Sbjct: 237 TTVQQTRELVTHALGSQLHADVPVCSLLSGGLDSTTLTAIAQRIVKAKTGGNINSFSVDF 296

Query: 290 PGK---------EIDESKYQRIVVDECRVSWYRTTFNVEDVLADII---DLIRTQEEPFS 337
            G+           D+ +   ++  E   S ++T     + L   +   ++ R +E PF+
Sbjct: 297 TGQAEQFKSDDLRPDQDQPFALLAAEFIGSRHQTVLIDNEELVSYLAREEVFRARELPFT 356

Query: 338 TLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREIL 397
              +     ++         V + G+G+DE+  GY +F     +     F W   ++   
Sbjct: 357 FGDMDTSLNLLFREIRKHSTVAISGEGADEVFGGYGWFRDPKVI-ASASFPWSSRVK--- 412

Query: 398 AYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLN 457
                                              L   FI     R      ++  + +
Sbjct: 413 -----------------------------------LPAGFINADFNRQCDLAQYQQASYD 437

Query: 458 EALLLAE-TYYSLPHLLRFED---KNAMRWSI-----ESRVPFC----------DHELVE 498
           +ALL  E      PH  R  +    +  RW +     + R+  C          DHELVE
Sbjct: 438 DALLQVEHLAQDSPHERRMRELCHMHLKRWMVMLLDRKDRLSMCNSLEVRVPFTDHELVE 497

Query: 499 YVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNR 533
           Y+ ++P   K   G  K LL++     +P+ +  R
Sbjct: 498 YIYNVPWSIKSKDGEEKWLLKQACADFVPEAVLKR 532


>sp|Q10374|ASNH_MYCTU PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]
           >gi|7477893|pir||B70785 probable asparagine synthetase -
           Mycobacterium tuberculosis  (strain H37RV)
           >gi|1237054|emb|CAA94270.1| (Z70283) asnB [Mycobacterium
           tuberculosis]
           Length = 652
           
 Score =  180 bits (452), Expect = 4e-44
 Identities = 172/623 (27%), Positives = 286/623 (45%), Gaps = 52/623 (8%)

Query: 15  VKRMNDAIRHRGPDDEGVY-----VDDNVSLGHVRLAIIDLSPKGHQPMKY---EKDGKE 66
           + R +  +RHRGPD+ G +         V  G  RL+IID++   HQP+++   E   + 
Sbjct: 26  IARASHLMRHRGPDESGTWHAVDGASGGVVFGFNRLSIIDIA-HSHQPLRWGPPEAPDRY 84

Query: 67  VWIVYNGEIYNFMEIRKELE-EKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIY 125
           V +V+NGEIYN++E+R EL  + G  F ++ D E ILA Y  WG + ++R  GM+AF ++
Sbjct: 85  V-LVFNGEIYNYLELRDELRTQHGAVFATDGDGEAILAGYHHWGTEVLQRLRGMFAFALW 143

Query: 126 DKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFD------- 178
           D     LF +RD FGIKPL+          +SE K +L         + V FD       
Sbjct: 144 DTVTRELFCARDPFGIKPLFIATGAGGTAVASEKKCLLD------LVELVGFDTEIDHRA 197

Query: 179 FLYYNLLDHT--EDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQ 236
             +Y +L +    +T   G++RL  G  A    +       +Y+  R         +D  
Sbjct: 198 LQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRYFVPRFAASPITNDNDQA 257

Query: 237 GFKDL---FLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVFPGK 292
            + ++      +V   + ADV VG+ LSGG+DS++I   A+RH   +  +  F+  F  +
Sbjct: 258 RYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRH---NPRLITFTTGFERE 314

Query: 293 EIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLAR 352
              E        +           + ++ +A + +++   +EP +  ++   + V + AR
Sbjct: 315 GFSEIDVAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEPVADPALVPLFFVAREAR 374

Query: 353 ENGMKVLLDGQGSDEILAGYHYF--------FGYYFVELLRKF-KWGKLLREILAYKR-I 402
           ++ +KV+L G+G+DE+  GY  +        F Y    L R   K  K L E +  K  +
Sbjct: 375 KH-VKVVLSGEGADELFGGYTIYREPLSLRPFDYLPKPLRRSMGKVSKPLPEGMRGKSLL 433

Query: 403 HGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRP--SKELIWKIKTLNEAL 460
           H   + L+         +   +L R   P  R ++       P  ++   W      + +
Sbjct: 434 HRGSLTLEERYYGNARSFSGAQL-REVLPGFRPDWTHTDVTAPVYAESAGWDPVARMQHI 492

Query: 461 LLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRK 520
            L    +    +L   DK  M  S+E RVPF D E+      LP  +K++   TK  LR+
Sbjct: 493 DLFT--WLRGDILVKADKITMANSLELRVPFLDPEVFAVASRLPAGAKITRTTTKYALRR 550

Query: 521 GLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMF 580
            L+ I+P  + +R  K+GF  P +  L+  E  ++A A + S   +     D   V RM 
Sbjct: 551 ALEPIVPAHVLHR-PKLGFPVPIRHWLRAGELLEWAYATVGSS--QAGHLVDIAAVYRML 607

Query: 581 EEHVSGKKNWSQELWKVIIMELW 603
           +EH  G  + S+ LW ++I  LW
Sbjct: 608 DEHRCGSSDHSRRLWTMLIFMLW 630


>gb|AAG02363.1|AF210249_22 (AF210249) asparagine synthetase [Streptomyces verticillus]
           Length = 638
           
 Score =  176 bits (441), Expect = 8e-43
 Identities = 176/575 (30%), Positives = 256/575 (43%), Gaps = 55/575 (9%)

Query: 1   MCGINGFSWGDE-----ELVKRMNDAIRHRGPDDEGVYVDDN--VSLGHVRLAIIDLSPK 53
           +CGI      D      EL   M D +R RGPD EG +V      +LGH RLA+I     
Sbjct: 4   VCGIVAIRSADGGLDGGELTAPMAD-LRPRGPDGEGTWVSPTGRAALGHTRLAVI-APDA 61

Query: 54  GHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV 113
           G QP+    DG  V +V NGE Y + EIR EL   G  F + +D+E+ L  YL  G   +
Sbjct: 62  GRQPVA-GPDGT-VRLVVNGEFYGYREIRAELRAAGCRFRTGSDSEIALHLYLRDGRRAL 119

Query: 114 ERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPND 173
           ER  G +AFV++D+ +  LF +RDRFG+KPLYY      +  +S ++ +L    P + + 
Sbjct: 120 ERLRGEFAFVLWDERRATLFAARDRFGVKPLYYTERDGRLYVASTVRALLSCGAPARWDT 179

Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLN-TRKFEAWKY-YDLRERIEKRKE 231
           A     L   L    + T F GI++L PG + + D + TR    W   Y     +  R  
Sbjct: 180 AAFAAHLQLGL--PPDRTLFAGIRQLPPGCHLIADAHGTRVTPYWDLDYPPAGELAARGS 237

Query: 232 ISDP-QGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLVF 289
           + D     ++    AV++R VADVP+   LSGGLDSS++   A RH      +  F++ F
Sbjct: 238 LDDHLDAVRERTDEAVRLRTVADVPLACHLSGGLDSSAVAASAARHT----RLTAFTVRF 293

Query: 290 PGKEIDESKYQR-----IVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
                DES   R     + +D   V+  R  F   D L D++      +E    ++ Y  
Sbjct: 294 DDPAFDESAVARRTAAHLAIDHREVASERAHF--ADHLRDVVRAGEMVQENSHGIARYLH 351

Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRI-- 402
              +K A   G   +L G+G DE+  GY  F     + L    +  K  R    Y R+  
Sbjct: 352 SAHIKKA---GFTAVLAGEGGDELFLGYPQFRKDLTLSLSADAR-DKADR---GYARLVA 404

Query: 403 HGSLVP-LKNMV--LYILPVWVTKRLWRRRFPY---LREEFIEKFKKRPSKE-------- 448
            G L P L+ ++  L  LP W+  R      P    LR +F  +  +  +          
Sbjct: 405 AGLLPPYLRTLLGTLGFLPSWIVDRHLAVTQPVAALLRPDFAAELARADAAAPLLAAGAG 464

Query: 449 -LIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPES 507
            L  +        L A+T+  LP  L   ++     ++E R+P  DH L + V   PP  
Sbjct: 465 LLAGRAPAHQATYLFAKTW--LPGYLLAAERLDAAQAVEVRLPLFDHHLFDLVRHTPPAW 522

Query: 508 KVSAGITKVLLRKGLKGILPDEIRNRASKVGFATP 542
               G  K  LR  ++  LP E+     K GF  P
Sbjct: 523 YDKDGTGKYPLRAAMRHRLPREV-TEGRKQGFLAP 556


>sp|P42113|ASNH_BACSU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2
           >gi|7474448|pir||A69591 asparagine synthetase asnH -
           Bacillus subtilis >gi|904192|dbj|BAA21593.1| (AB005554)
           probable asparagine synthetase [Bacillus subtilis]
           >gi|2636538|emb|CAB16028.1| (Z99124) asparagine
           synthetase (glutamine-hydrolyzing) [Bacillus subtilis]
           Length = 747
           
 Score =  170 bits (427), Expect = 4e-41
 Identities = 162/547 (29%), Positives = 258/547 (46%), Gaps = 66/547 (12%)

Query: 14  LVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNG 73
           ++K M DAI +RGPDDE  ++D  V     RL+I+DL   G QP   E DG  V ++ NG
Sbjct: 22  ILKGMADAISYRGPDDEQYHIDSKVGFAFRRLSILDLV-NGQQPFLNE-DGSIV-VMVNG 78

Query: 74  EIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILF 133
           EIYN+ E++  L    + F + +D EVI+  Y E G   V+   GM++  I+DK+KN +F
Sbjct: 79  EIYNYKELKASLHN--HMFKTTSDCEVIVHLYEEKGIGFVDDIIGMFSIAIWDKNKNKVF 136

Query: 134 LSRDRFGIKPLYYYYDGKNIIFSSEIKGILQH-NIPRKPNDAVIFDFLYYN---LLDHTE 189
           L RDRFGIKPL+Y      +IF+SEIK +  H + PR+ N       +  +     +H E
Sbjct: 137 LVRDRFGIKPLFYTELKHELIFASEIKSLFSHPHCPRQFNWKEALSDICLSGEAASNHKE 196

Query: 190 DT-FFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKR--KEISDP----QGFKDLF 242
            T FF  I+ L  G+    +L T + +   Y+ L++ + ++  +E   P    +G+++L 
Sbjct: 197 TTSFFVNIQNLDAGHYLEINLTTNERKTASYWSLQDILLRQGYRENLHPDDLIEGYRELL 256

Query: 243 LRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIKVFSLVFPGKEIDE-SKYQR 301
             +V   L +DV VG  LSGG+DS+++           ++  F+++      +E +KY  
Sbjct: 257 ADSVHRCLQSDVEVGLFLSGGIDSAAVA---HFAAEKQDLHTFTVLSQSTFTNEDAKYAH 313

Query: 302 IVVDECRVSWYRTTFNV--EDVL--ADIIDLIRTQEEPF---STLSIYGQYRVMKLAREN 354
            +  +  +  ++  + +  +++L       L+   E PF     L  +  ++  K  R N
Sbjct: 314 WLAKDLHLPNHQVLYQLGNDELLQPESYKHLLWICETPFCGPEQLYKFHLHKYAKAIRPN 373

Query: 355 GMKVLLDGQGSDEILAGYHYFFG-------YYFVELLRKFKWGKLLREILAYKRIHGSLV 407
            +KV+L GQGSDE   GY              F+E +   +  +L        R+ G++ 
Sbjct: 374 -LKVMLTGQGSDEFNGGYSTTLSPAENPSWEGFIESVNTMEMNRL-------HRLQGNIF 425

Query: 408 PLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKEL-IWKIKTLNEALLLAETY 466
                           R+W   F  L    +   K   S +   W+   L +   L    
Sbjct: 426 ----------------RVWEEHFG-LSPINLSYLKSNDSSQADPWQSYVLTKYRDL--QM 466

Query: 467 YSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGIL 526
           Y+  H    ED+ A    IE+RVPF DH LVE+V  +P   +      K +LRK L   L
Sbjct: 467 YNCWH----EDRIAAANHIENRVPFLDHRLVEWVCGIPDGLRKDLLWDKSVLRKSLTNEL 522

Query: 527 PDEIRNR 533
                +R
Sbjct: 523 HTSYTHR 529


>pir||H82255 asparagine synthetase B, glutamine-hydrolyzing VC0991 [imported] -
           Vibrio cholerae (group O1 strain N16961)
           >gi|9655452|gb|AAF94152.1| (AE004180) asparagine
           synthetase B, glutamine-hydrolyzing [Vibrio cholerae]
           Length = 554
           
 Score =  143 bits (358), Expect = 4e-33
 Identities = 119/375 (31%), Positives = 179/375 (47%), Gaps = 54/375 (14%)

Query: 18  MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYN 77
           M+  +RHRGPD  G+Y  D   L H RLAI+ L+  G QP+ Y  D K +  V NGEIYN
Sbjct: 24  MSKKLRHRGPDWSGIYASDKAILAHERLAIVGLN-SGAQPL-YSPDRKLILAV-NGEIYN 80

Query: 78  FMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRD 137
             EIR+  + + Y F +++D EVILA Y + G D +E  NG++AFV+YD+ K+   + RD
Sbjct: 81  HKEIRERYKGQ-YEFQTDSDCEVILALYRDKGADLLEELNGIFAFVLYDEEKDEYLIGRD 139

Query: 138 RFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGI 196
             GI PLY  +D   N   +SE+K ++       P    + +F   + L   +       
Sbjct: 140 HIGIIPLYQGHDEHGNFYVASEMKALV-------PVCKTLSEFPPGSYLSSKDQAV---- 188

Query: 197 KRLMPGYNAVFDLNTRKF-EAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVP 255
                         TR +   W  +D     E +  +S  +        AVK +L+ DVP
Sbjct: 189 --------------TRYYVRDWNSFD-----EVKDNVSSKEELTQALEAAVKRQLMTDVP 229

Query: 256 VGSCLSGGLDSS------SIVCAMRHLLPDGE------IKVFSLVFPGKEIDESKYQRIV 303
            G  LSGGLDSS          AMR  + D E       ++ S     +   + K  R V
Sbjct: 230 YGVLLSGGLDSSITSAIAKRFAAMR--IEDDEKSAAWWPQLHSFAIGLEGAPDLKAAREV 287

Query: 304 VDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLL 360
            ++     +  T+ +++ L  I D+I    E +   +I     +  + R+    G+K++L
Sbjct: 288 AEKIGTVHHEMTYTIQEGLDAIRDVI-YHIETYDVTTIRASTPMFLMGRKIKAMGIKMVL 346

Query: 361 DGQGSDEILAGYHYF 375
            G+G+DEI  GY YF
Sbjct: 347 SGEGADEIFGGYLYF 361


>sp|P22106|ASNB_ECOLI ASPARAGINE SYNTHETASE B [GLUTAMINE-HYDROLYZING]
           >gi|68659|pir||AJECN asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) - Escherichia coli
           >gi|145393|gb|AAA23498.1| (J05554) asparagine synthetase
           B [Escherichia coli] >gi|1651277|dbj|BAA35317.1|
           (D90706) Asparagine synthase (glutamine-hydrolyzing) (EC
           6.3.5.4) [Escherichia coli] >gi|1786889|gb|AAC73768.1|
           (AE000171) asparagine synthetase B [Escherichia coli
           K12]
           Length = 554
           
 Score =  139 bits (346), Expect = 1e-31
 Identities = 119/370 (32%), Positives = 175/370 (47%), Gaps = 52/370 (14%)

Query: 22  IRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEI 81
           +RHRGPD  G+Y  DN  L H RL+I+D++  G QP+ Y +    V  V NGEIYN   +
Sbjct: 28  MRHRGPDWSGIYASDNAILAHERLSIVDVNA-GAQPL-YNQQKTHVLAV-NGEIYNHQAL 84

Query: 82  RKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGI 141
           R E  ++ Y F + +D EVILA Y E G + ++   GM+AF +YD  K+   + RD  GI
Sbjct: 85  RAEYGDR-YQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGI 143

Query: 142 KPLYYYYDGKNIIF-SSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLM 200
            PLY  YD    ++ +SE+K ++       P    I +F   + L   +        R  
Sbjct: 144 IPLYMGYDEHGQLYVASEMKALV-------PVCRTIKEFPAGSYLWSQDGEIRSYYHRDW 196

Query: 201 PGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCL 260
             Y+AV D  T K E      LR+ +E                 +VK  L++DVP G  L
Sbjct: 197 FDYDAVKDNVTDKNE------LRQALED----------------SVKSHLMSDVPYGVLL 234

Query: 261 SGGLDSSSIVCAMR----HLLPDGE--------IKVFSLVFPGKEIDESKYQRIVVDECR 308
           SGGLDSS I    +      + D E        +  F++  PG    + K  + V +   
Sbjct: 235 SGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGS--PDLKAAQEVANHLG 292

Query: 309 VSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGS 365
              +   F V+   D + D+I  I T +      S    Y + +  +  G+K++L G+GS
Sbjct: 293 TVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRAST-PMYLMSRKIKAMGIKMVLSGEGS 351

Query: 366 DEILAGYHYF 375
           DE+  GY YF
Sbjct: 352 DEVFGGYLYF 361


>pdb|1CT9|A Chain A, Crystal Structure Of Asparagine Synthetase B From
           Escherichia Coli >gi|6730196|pdb|1CT9|B Chain B, Crystal
           Structure Of Asparagine Synthetase B From Escherichia
           Coli >gi|6730197|pdb|1CT9|C Chain C, Crystal Structure
           Of Asparagine Synthetase B From Escherichia Coli
           >gi|6730198|pdb|1CT9|D Chain D, Crystal Structure Of
           Asparagine Synthetase B From Escherichia Coli
           Length = 553
           
 Score =  139 bits (346), Expect = 1e-31
 Identities = 119/370 (32%), Positives = 175/370 (47%), Gaps = 52/370 (14%)

Query: 22  IRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEI 81
           +RHRGPD  G+Y  DN  L H RL+I+D++  G QP+ Y +    V  V NGEIYN   +
Sbjct: 27  MRHRGPDWSGIYASDNAILAHERLSIVDVNA-GAQPL-YNQQKTHVLAV-NGEIYNHQAL 83

Query: 82  RKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGI 141
           R E  ++ Y F + +D EVILA Y E G + ++   GM+AF +YD  K+   + RD  GI
Sbjct: 84  RAEYGDR-YQFQTGSDCEVILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGI 142

Query: 142 KPLYYYYDGKNIIF-SSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLM 200
            PLY  YD    ++ +SE+K ++       P    I +F   + L   +        R  
Sbjct: 143 IPLYMGYDEHGQLYVASEMKALV-------PVCRTIKEFPAGSYLWSQDGEIRSYYHRDW 195

Query: 201 PGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCL 260
             Y+AV D  T K E      LR+ +E                 +VK  L++DVP G  L
Sbjct: 196 FDYDAVKDNVTDKNE------LRQALED----------------SVKSHLMSDVPYGVLL 233

Query: 261 SGGLDSSSIVCAMR----HLLPDGE--------IKVFSLVFPGKEIDESKYQRIVVDECR 308
           SGGLDSS I    +      + D E        +  F++  PG    + K  + V +   
Sbjct: 234 SGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLPGS--PDLKAAQEVANHLG 291

Query: 309 VSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGS 365
              +   F V+   D + D+I  I T +      S    Y + +  +  G+K++L G+GS
Sbjct: 292 TVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRAST-PMYLMSRKIKAMGIKMVLSGEGS 350

Query: 366 DEILAGYHYF 375
           DE+  GY YF
Sbjct: 351 DEVFGGYLYF 360


>dbj|BAA89376.1| (AB025342) ORF2 [Moritella marina]
           Length = 555
           
 Score =  137 bits (342), Expect = 3e-31
 Identities = 120/379 (31%), Positives = 180/379 (46%), Gaps = 52/379 (13%)

Query: 13  ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYN 72
           E    ++  +RHRGPD  G+Y  DN  L H RLAI+D++  G QP+  E+  K   +  N
Sbjct: 19  EQALELSKLLRHRGPDWSGIYTSDNAILVHERLAIVDVN-NGAQPLYNEE--KTHVLAVN 75

Query: 73  GEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNIL 132
           GEIYN  +++K L    + F + +D EVILA Y E G   ++  NG++AF +YD++++  
Sbjct: 76  GEIYNHKDLKKTLNVD-FEFQTESDCEVILALYKEKGTQFLDDLNGIFAFALYDETEDAY 134

Query: 133 FLSRDRFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDT 191
            + RD  GI PLY  YD   N   +SE+K ++       P    I +F   + L   +  
Sbjct: 135 LIGRDHIGIIPLYTGYDEHGNFYVASEMKALV-------PICTQIEEFPAGHYLWSKDGV 187

Query: 192 FFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLV 251
                  + P Y             WK +D   +    K +   QG +D    AVK +L+
Sbjct: 188 -------VTPYYQ----------RDWKDFDNVAQNGGDKSVV-KQGLED----AVKRQLM 225

Query: 252 ADVPVGSCLSGGLDSSSIVCA----MRHLLPDG--------EIKVFSLVFPGKEIDESKY 299
            DVP G  LSGGLDSS I        +  + DG        ++  FS+   G   D +  
Sbjct: 226 CDVPYGVLLSGGLDSSVISAITQQYAKRRIEDGGKTEAWWPQLHSFSVGLNGSP-DLAAA 284

Query: 300 QRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGM 356
           Q+ V D      +   F V+   D L D+I  I T +      S    Y + +  +  G+
Sbjct: 285 QK-VADHLGTIHHSIEFTVQEGIDALRDVIYHIETYDVTTIRAST-PMYLMARKIKAMGI 342

Query: 357 KVLLDGQGSDEILAGYHYF 375
           K++L G+G+DE+  GY YF
Sbjct: 343 KMVLSGEGADELFGGYLYF 361


>gb|AAB99117.1| (U67554) asparagine synthetase (asnB) [Methanococcus jannaschii]
           Length = 544
           
 Score =  136 bits (338), Expect = 1e-30
 Identities = 120/409 (29%), Positives = 195/409 (47%), Gaps = 72/409 (17%)

Query: 1   MCGINGFSWGDEELVKR----MNDAIRHRGPDDEGVYVDD-------------------- 36
           MC I+G    D ++  +    M   ++HRG D+ G+ +DD                    
Sbjct: 4   MCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIG 63

Query: 37  NVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNT 96
           N+SL H RLAI+     G QP+  E +   +W+V NGEIYN++E+R+ L++  + F +++
Sbjct: 64  NLSLAHNRLAIV--GRYGVQPIPNEDE--TIWLVCNGEIYNYIELREYLKQN-HEFRTDS 118

Query: 97  DTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFS 156
           D EVI+  Y E   + +E  +G +AF IYDKSKN++ L+RD FG+KPL+Y    K   F+
Sbjct: 119 DNEVIIHLYEE---EKLEELDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVDRDKYFAFA 175

Query: 157 SEIKGI--LQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKF 214
           SE K +  L  NI     D                D     IK L P    ++ L+  +F
Sbjct: 176 SERKALWHLLINIDGCERDL---------------DELNSKIKTLKPNSQLIYYLDDNRF 220

Query: 215 EAWK-YYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADV-PVGSCLSGGLDSS----- 267
           E  + +  L     K +   + + + D  L+   ++ V  +  VG   SGG+DSS     
Sbjct: 221 EIIEGFKKLELNYMKERSYEEAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKL 280

Query: 268 -SIVCAMRHLLPDGEIKVFSLVFP---GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLA 323
            S+ C +  L   G      L++     K+++    ++I+ +E    +    F V   + 
Sbjct: 281 ASLYCEV-ILYAVGTENSEDLIYAERLAKDLNLKLRKKIISEE---EYEEYVFKVAKAI- 335

Query: 324 DIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
           D +DL++              Y   ++A E+G+KV+L GQG+DE+  GY
Sbjct: 336 DEVDLMKIGVG-------IPIYVASEMANEDGLKVVLSGQGADELFGGY 377


>sp|Q58516|ASNH_METJA PUTATIVE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]
           >gi|11272681|pir||C64439 asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) MJ1116 [similarity]
           - Methanococcus jannaschii
           Length = 541
           
 Score =  136 bits (338), Expect = 1e-30
 Identities = 120/409 (29%), Positives = 195/409 (47%), Gaps = 72/409 (17%)

Query: 1   MCGINGFSWGDEELVKR----MNDAIRHRGPDDEGVYVDD-------------------- 36
           MC I+G    D ++  +    M   ++HRG D+ G+ +DD                    
Sbjct: 1   MCSISGIIVKDNQISAKYSIDMMKILKHRGRDNSGLLLDDEVIYFNDFEDVEDLEEEMIG 60

Query: 37  NVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNT 96
           N+SL H RLAI+     G QP+  E +   +W+V NGEIYN++E+R+ L++  + F +++
Sbjct: 61  NLSLAHNRLAIV--GRYGVQPIPNEDE--TIWLVCNGEIYNYIELREYLKQN-HEFRTDS 115

Query: 97  DTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFS 156
           D EVI+  Y E   + +E  +G +AF IYDKSKN++ L+RD FG+KPL+Y    K   F+
Sbjct: 116 DNEVIIHLYEE---EKLEELDGDYAFAIYDKSKNVVRLARDMFGVKPLFYVDRDKYFAFA 172

Query: 157 SEIKGI--LQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKF 214
           SE K +  L  NI     D                D     IK L P    ++ L+  +F
Sbjct: 173 SERKALWHLLINIDGCERDL---------------DELNSKIKTLKPNSQLIYYLDDNRF 217

Query: 215 EAWK-YYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADV-PVGSCLSGGLDSS----- 267
           E  + +  L     K +   + + + D  L+   ++ V  +  VG   SGG+DSS     
Sbjct: 218 EIIEGFKKLELNYMKERSYEEAKEYLDRALKNSVLKRVRGLDKVGIICSGGVDSSLIAKL 277

Query: 268 -SIVCAMRHLLPDGEIKVFSLVFP---GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLA 323
            S+ C +  L   G      L++     K+++    ++I+ +E    +    F V   + 
Sbjct: 278 ASLYCEV-ILYAVGTENSEDLIYAERLAKDLNLKLRKKIISEE---EYEEYVFKVAKAI- 332

Query: 324 DIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
           D +DL++              Y   ++A E+G+KV+L GQG+DE+  GY
Sbjct: 333 DEVDLMKIGVG-------IPIYVASEMANEDGLKVVLSGQGADELFGGY 374


>emb|CAA96526.1| (Z72354) asparagine synthetase [Vicia faba]
           Length = 586
           
 Score =  135 bits (336), Expect = 2e-30
 Identities = 115/391 (29%), Positives = 181/391 (45%), Gaps = 51/391 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+RK+L    +TF +  D +VI   Y E+G + V+
Sbjct: 60  DQPLFNED--KSIIVTVNGEIYNHEELRKQLPN--HTFRTQCDCDVIAHLYEEYGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  +SE+KG+         N
Sbjct: 116 LLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++    R+F  W               
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKEREFRRWYNPPWFNEAIIPSTP 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
            DP   +  F +AV  RL+ DVP G  LSGGLDSS +       L D +       K+ S
Sbjct: 206 YDPLVLRTAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLADTKAAKQWGAKLHS 265

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
                K   + K  + V D      +   F ++D +  I D+I  T+    +T+ +    
Sbjct: 266 FCVGLKGAPDLKAGKEVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325

Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           + + +  + +G+K+L+ G+GSDEI  GY YF
Sbjct: 326 FLMSRKIKSSGVKMLISGEGSDEIFGGYLYF 356


>gb|AAF02775.1|AF190728_1 (AF190728) asparagine synthetase [Helianthus annuus]
           Length = 591
           
 Score =  133 bits (332), Expect = 5e-30
 Identities = 122/394 (30%), Positives = 177/394 (43%), Gaps = 56/394 (14%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G+Y   +  L H RLAIID +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHGDNYLSHQRLAIIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   + + +  NGEIYN  E+RK L   G+ F + +D +VI   Y E G D V+
Sbjct: 60  DQPLFNED--QTIVVTVNGEIYNHEELRKRLTAAGHKFRTGSDCDVIAHLYEEHGEDFVD 117

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM++FV+ D   N    +RD  GI  LY  +  DG ++  SSE+KG+         N
Sbjct: 118 MLDGMFSFVLLDTRNNTYIAARDAIGITSLYIGWGLDG-SVWISSELKGL---------N 167

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++   T  F+ W Y  L          
Sbjct: 168 DDC------------------EHFEVFPPGH--LYSSKTGGFKRW-YNPLWFAETIPSTP 206

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLP--------DGEIKV 284
            DP   +  F  AV  RL+ DVP G  LSGGLDSS +       L           ++  
Sbjct: 207 YDPLVLRCAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTKAAKQWGAQLHS 266

Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
           F +   G    + K  R V D      +   F V+D +  I D+I    E +   +I   
Sbjct: 267 FCVGLEGS--PDLKAAREVADYLGTVHHEFHFTVQDGIDAIEDVI-YHIETYDVTTIRAS 323

Query: 345 YRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
             +  ++R+    G+K+++ G+GSDEI  GY YF
Sbjct: 324 TPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 357


>dbj|BAA96252.1| (AB035248) asparagine synthetase [Astragalus sinicus]
           Length = 586
           
 Score =  133 bits (332), Expect = 5e-30
 Identities = 117/395 (29%), Positives = 186/395 (46%), Gaps = 60/395 (15%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  ++  K + +  NGEIYN  E+RK+L    + F ++ D +VI   Y E+G + V+
Sbjct: 60  DQPLFNQE--KSIIVTVNGEIYNHEELRKQLPN--HKFRTDCDCDVIAHLYEEYGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  +SE+KG+         N
Sbjct: 116 MLDGIFSFVVLDTRDNSFLVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
           D                    E  +   PG+  ++    R+F  W   K++   E +   
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKEREFRRWYNPKWFS--ETVPSS 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IK 283
               DP   +  F +AV  RL++DVP G  LSGGLDSS +       L D +       K
Sbjct: 204 P--YDPLVLRHAFEKAVIKRLMSDVPFGVLLSGGLDSSLVASVTARYLEDTKAAKQWGAK 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
           + +     K   + K  R V D      +   F ++D +  I D+I    E F   +I  
Sbjct: 262 LHTFCVGLKGAPDLKAGREVADYLGTVHHEFEFTIQDGIDAIEDVI-YHTETFDVTTIRA 320

Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
              +  ++R+    G+K++L G+GSDEI  GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355


>gb|AAF02776.1|AF190729_1 (AF190729) asparagine synthetase [Helianthus annuus]
           Length = 558
           
 Score =  133 bits (331), Expect = 6e-30
 Identities = 120/394 (30%), Positives = 180/394 (45%), Gaps = 58/394 (14%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI   F   D    KR     ++  +RHRGPD  G++ +++  L H RLAI+D +  G
Sbjct: 1   MCGILAVFGCVDCSQAKRARILELSSRLRHRGPDWSGLHSEEDCYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K + +  NGEIYN  E R++L  K + F + +D EVI   Y E G D V 
Sbjct: 60  DQPL-YNED-KSIIVTVNGEIYNHKEFREQL--KSHKFNTGSDCEVIAHLYEEHGEDFVH 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM++FV+ D        +RD  GI PLY  +  DG ++ F+SE+K +          
Sbjct: 116 MLDGMFSFVLLDTRNKSYIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                          ++D   E     +PG+  ++         W        +      
Sbjct: 165 ---------------SDDC--EQFMSFLPGH--IYSSKNGGLRRWYNPPWWSELVPSTPY 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GEIKV 284
            DP   ++ F +AV  RL+ DVP G  LSGGLDSS +   A RHL+          ++  
Sbjct: 206 -DPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVASRHLVDSEAYCQWGSQLHT 264

Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSI 341
           F +   G    +    R V D      +   F V+   D L ++I  I T +      S 
Sbjct: 265 FCIGLKGS--PDLVAAREVADYLGTRHHEFYFTVQEGIDALEEVIYHIETYDVTTIRAST 322

Query: 342 YGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
              + + +  +  G+K++L G+GSDEI  GY YF
Sbjct: 323 -PMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355


>dbj|BAA97313.1| (AB019236) asparagine synthetase [Arabidopsis thaliana]
           Length = 578
           
 Score =  132 bits (329), Expect = 1e-29
 Identities = 120/394 (30%), Positives = 182/394 (45%), Gaps = 58/394 (14%)

Query: 1   MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI         S      +  ++  +RHRGPD  G++  ++  L H RLAIID +  G
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPT-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K V +  NGEIYN   +R++L  K + F + +D EVI   Y E G + ++
Sbjct: 60  DQPL-YNED-KTVAVTVNGEIYNHKILREKL--KSHQFRTGSDCEVIAHLYEEHGEEFID 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM+AFV+ D        +RD  GI PLY  +  DG ++ F+SE+K +          
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                          ++D   E      PG+  ++         W Y       +     
Sbjct: 165 ---------------SDDC--EQFMSFPPGH--IYSSKQGGLRRW-YNPPWYNEQVPSTP 204

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GEIKV 284
            DP   ++ F +AV  RL+ DVP G  LSGGLDSS +   A+RHL           ++  
Sbjct: 205 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHT 264

Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
           F +   G    + K  R V D      +   F V+D + D I+ +    E +   +I   
Sbjct: 265 FCIGLQGS--PDLKAGREVADYLGTRHHEFQFTVQDGI-DAIEEVIYHIETYDVTTIRAS 321

Query: 345 YRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
             +  ++R+    G+K++L G+GSDEIL GY YF
Sbjct: 322 TPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 355


>gb|AAC72837.1| (AF095453) asparagine synthetase [Arabidopsis thaliana]
           Length = 578
           
 Score =  132 bits (329), Expect = 1e-29
 Identities = 120/394 (30%), Positives = 182/394 (45%), Gaps = 58/394 (14%)

Query: 1   MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI         S      +  ++  +RHRGPD  G++  ++  L H RLAIID +  G
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPT-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K V +  NGEIYN   +R++L  K + F + +D EVI   Y E G + ++
Sbjct: 60  DQPL-YNED-KTVAVTVNGEIYNHKILREKL--KSHQFRTGSDCEVIAHLYEEHGEEFID 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM+AFV+ D        +RD  GI PLY  +  DG ++ F+SE+K +          
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                          ++D   E      PG+  ++         W Y       +     
Sbjct: 165 ---------------SDDC--EQFMSFPPGH--IYSSKQGGLRRW-YNPPWYNEQVPSTP 204

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GEIKV 284
            DP   ++ F +AV  RL+ DVP G  LSGGLDSS +   A+RHL           ++  
Sbjct: 205 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVALRHLEKSEAARQWGSQLHT 264

Query: 285 FSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ 344
           F +   G    + K  R V D      +   F V+D + D I+ +    E +   +I   
Sbjct: 265 FCIGLQGS--PDLKAGREVADYLGTRHHEFQFTVQDGI-DAIEEVIYHIETYDVTTIRAS 321

Query: 345 YRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
             +  ++R+    G+K++L G+GSDEIL GY YF
Sbjct: 322 TPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYF 355


>gi|4502259 asparagine synthetase [Homo sapiens]
           >gi|114266|sp|P08243|ASNS_HUMAN ASPARAGINE SYNTHETASE
           [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT ASPARAGINE
           SYNTHETASE) (TS11 CELL CYCLE CONTROL PROTEIN)
           >gi|68655|pir||AJHUN1 asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) - human
           >gi|179100|gb|AAA51789.1| (M27396) asparagine synthetase
           [Homo sapiens] >gi|533200|gb|AAA52756.1| (L35946)
           asparagine synthetase [Homo sapiens]
           Length = 561
           
 Score =  131 bits (326), Expect = 2e-29
 Identities = 160/624 (25%), Positives = 255/624 (40%), Gaps = 116/624 (18%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI      D+ L  +   A++  HRGPD    E V    N   G  RLA++D    G 
Sbjct: 1   MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
           QP++ +K    +W+ YNGEIYN  ++++  E   + + +  D E+IL  Y + G +  + 
Sbjct: 60  QPIRVKKY-PYLWLCYNGEIYNHKKMQQHFE---FEYQTKVDGEIILHLYDKGGIEQTIC 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
             +G++AFV+ D +   +FL RD +G++PL+        +   SE KG++          
Sbjct: 116 MLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVT--------- 166

Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
                      L H+   F + ++  +PG+  V DL         E  KY          
Sbjct: 167 -----------LKHSATPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHA 214

Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
            YD  E++    EI   +   + LF  AVK RL+ D  +G  LSGGLDSS +   +   L
Sbjct: 215 LYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQL 274

Query: 278 PDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIIDLIRTQ 332
            + +++  + +     ++  +    R V D      Y   FN E+    L ++I  + T 
Sbjct: 275 KEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY 334

Query: 333 EEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGK 391
           +      S+ G Y + K  R+N    V+  G+GSDE+  GY YF      E   + +  +
Sbjct: 335 DITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESER 392

Query: 392 LLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIW 451
           LLRE+  +  +        + +               R P+L   F   +   P +  I 
Sbjct: 393 LLRELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFFSYYLSLPPEMRI- 438

Query: 452 KIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSA 511
             K   E  LL ET         FED N                L+   +   P+   S 
Sbjct: 439 -PKNGIEKHLLRET---------FEDSN----------------LIPKEILWRPKEAFSD 472

Query: 512 GITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYW 571
           GIT V  +     IL + + ++           D +  N  QKF        + K +  +
Sbjct: 473 GITSV--KNSWFKILQEYVEHQVD---------DAMMANAAQKFPF-----NTPKTKEGY 516

Query: 572 DYRKVLRMFEEHVSGKKNWSQELW 595
            YR+V   FE H  G+ +W    W
Sbjct: 517 YYRQV---FERHYPGRADWLSHYW 537


>sp|O24661|ASNS_TRIVS ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|2429280|gb|AAD05033.1|
           (AF014055) asparagine synthetase [Triphysaria
           versicolor] >gi|2429282|gb|AAD05034.1| (AF014056)
           asparagine synthetase [Triphysaria versicolor]
           >gi|2429284|gb|AAD05035.1| (AF014057) asparagine
           synthetase [Triphysaria versicolor]
           Length = 586
           
 Score =  131 bits (325), Expect = 3e-29
 Identities = 124/395 (31%), Positives = 180/395 (45%), Gaps = 60/395 (15%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGIHHHGDCYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+R  L    + F + +D +VI   Y E+G + VE
Sbjct: 60  DQPLFNED--KRIAVTVNGEIYNHEELRALLPN--HKFRTGSDCDVIAHLYEEYGENFVE 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM++FV+ D   N    +RD FGI  LY  +  DG ++  SSE+KG+          
Sbjct: 116 MLDGMFSFVLLDSRDNTFIAARDAFGITSLYIGWGLDG-SVWISSELKGL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
                         H E   FE      PG+  V+   T  F  W  Y+     E I   
Sbjct: 165 --------------HDECENFEVFP---PGH--VYSSKTEGFRRW--YNPPWFSEAIPST 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIK----- 283
               DP   +  F +AV  RL+ DVP G  LSGGLDSS +     RHL      K     
Sbjct: 204 P--YDPLVLRGAFEQAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGTKAAKRWGSQ 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
           + S     +   + K  + V D      +   F V+D +  I D+I    E +   +I  
Sbjct: 262 LHSFCVGLEGSPDLKAGKEVADYLGTVHHEFLFTVQDGIDAIEDVI-YHIETYDVTTIRA 320

Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
              +  ++R+    G+K+++ G+GSDEI  GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355


>gi|11421677 asparagine synthetase [Homo sapiens] >gi|3341715|gb|AAC62263.1|
           (AC005326) asparagine synthetase [Homo sapiens]
           Length = 561
           
 Score =  130 bits (324), Expect = 4e-29
 Identities = 161/624 (25%), Positives = 255/624 (40%), Gaps = 116/624 (18%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI      D+ L  +   A++  HRGPD    E V    N   G  RLA++D    G 
Sbjct: 1   MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
           QP++ +K    +W+ YNGEIYN  ++++  E   + + +  D E+IL  Y + G +  + 
Sbjct: 60  QPIRVKKY-PYLWLCYNGEIYNHKKMQQHFE---FEYQTKVDGEIILHLYDKGGIEQTIC 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
             +G++AFV+ D +   +FL RD +G++PL+        +   SE KG++          
Sbjct: 116 MLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVT--------- 166

Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
                      L H+   F + ++  +PG+  V DL         E  KY          
Sbjct: 167 -----------LKHSATPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHA 214

Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
            YD  E++    EI   +   + LF  AVK RL+ D  +G  LSGGLDSS +   +   L
Sbjct: 215 LYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQL 274

Query: 278 PDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIIDLIRTQ 332
            + +++  + +     ++  +    R V D      Y   FN E+    L ++I  + T 
Sbjct: 275 KEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETY 334

Query: 333 EEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGK 391
           +      S+ G Y + K  R+N    V+  G+GSDE+  GY YF      E   + +  +
Sbjct: 335 DITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESER 392

Query: 392 LLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIW 451
           LLRE                  LY+  V    R        LR  F++            
Sbjct: 393 LLRE------------------LYLFDVLRADRTTAAHGLELRVPFLDH----------- 423

Query: 452 KIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSA 511
                      +  Y SLP  +R   KN +   +  R  F D  L+   +   P+   S 
Sbjct: 424 ---------RFSSYYLSLPPEMRI-PKNGIEKHL-LRETFEDSNLIPKEILWRPKEAFSD 472

Query: 512 GITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYW 571
           GIT V  +     IL + + ++           D +  N  QKF        + K +  +
Sbjct: 473 GITSV--KNSWFKILQEYVEHQVD---------DAMMANAAQKFPF-----NTPKTKEGY 516

Query: 572 DYRKVLRMFEEHVSGKKNWSQELW 595
            YR+V   FE H  G+ +W    W
Sbjct: 517 YYRQV---FERHYPGRADWLSHYW 537


>gb|AAC72836.1| (AF095452) asparagine synthetase [Arabidopsis thaliana]
           Length = 578
           
 Score =  130 bits (324), Expect = 4e-29
 Identities = 117/392 (29%), Positives = 180/392 (45%), Gaps = 54/392 (13%)

Query: 1   MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI         S      +  ++  +RHRGPD  G++  ++  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPT-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K + +  NGEIYN  ++R+ L  K + F + +D EVI   Y E G + V+
Sbjct: 60  DQPL-YNED-KTIAVTVNGEIYNHKDLRENL--KSHQFRTGSDCEVIAHLYEEHGEEFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM+AFV+ D        +RD  GI PLY  +  DG ++ F+SE+K +          
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                          ++D   E      PG+  ++         W        +      
Sbjct: 165 ---------------SDDC--EQFMCFPPGH--IYSSKQGGLRRWYNPPWFSEVVPSTPY 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEI-----KVFS 286
            DP   ++ F +AV  RL+ DVP+G  LSGGLDSS +   A+RHL           K+ +
Sbjct: 206 -DPLVVRNTFEKAVIKRLMTDVPLGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHT 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                K   + K  R V D      +   F V+D + D I+ +    E +   +I     
Sbjct: 265 FCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI-DAIEEVIYHVETYDVTTIRASTP 323

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K++L G+GSDEI  GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355


>pir||T08846 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) 2 -
           soybean >gi|1778370|gb|AAC49613.1| (U77678) asparagine
           synthetase 2 [Glycine max]
           Length = 581
           
 Score =  129 bits (320), Expect = 1e-28
 Identities = 120/395 (30%), Positives = 181/395 (45%), Gaps = 60/395 (15%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCFLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K V +  NGEIYN  E+RK+L    + F + +D +VI   Y E G D V+
Sbjct: 60  DQPLFNED--KSVIVTVNGEIYNHEELRKQLPN--HNFRTGSDCDVIAHLYEEHGEDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
           D                    E  +   PG+  ++    R F  W  Y+     E I   
Sbjct: 166 DDC------------------EHFECFPPGH--LYSSKERGFRRW--YNPPWFSEAIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
               DP   +  F +AV  RL+ DVP G  LSGGLDSS +       L + +       K
Sbjct: 204 P--YDPLVLRHAFEQAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLANTKAAEQWGSK 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
           + S     +   + K  + V D      +  TF V+D +  I D+I    E +   +I  
Sbjct: 262 LHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGIDSIEDVI-YHIETYDVTTIRA 320

Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
              +  ++R+    G+K ++ G+GSDEI  GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355


>pir||D82846 asparagine synthase B XF0118 [imported] - Xylella fastidiosa
           (strain 9a5c) >gi|9104900|gb|AAF82931.1|AE003865_8
           (AE003865) asparagine synthase B [Xylella fastidiosa]
           Length = 563
           
 Score =  129 bits (320), Expect = 1e-28
 Identities = 107/368 (29%), Positives = 171/368 (46%), Gaps = 49/368 (13%)

Query: 23  RHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIR 82
           RHRGPD  GVYVD    L H RLAI+D  P G       +DG  + +  NGEIYN   ++
Sbjct: 31  RHRGPDWSGVYVDVGAILVHERLAIVD--PAGGAQPLLSEDG-TLALAVNGEIYNHAVLK 87

Query: 83  KELEEKGYTFTSNTDTEVILAAYL-EWGFDCVERFNGMWAFVIYDKSKNILFLSRDRFGI 141
             L++  Y F +++D EVI A Y  E     + R NG++AF ++DK+     ++RD  G+
Sbjct: 88  GTLQQP-YVFQTHSDCEVINALYREETPTSFLNRLNGIFAFALWDKTTGRGLIARDPMGV 146

Query: 142 KPLYYYYD-GKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLM 200
            PLY+ +D    +  +SE+K +++H     P+ A      +Y+    T   ++E      
Sbjct: 147 VPLYWGHDQDGRLRVASEMKALIEH----CPDVAQFPPGHWYDTTTGTLVKYYE------ 196

Query: 201 PGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCL 260
                          +WK Y   E ++        Q  ++ F RAV  +L+ DVP G  L
Sbjct: 197 --------------RSWKNYSAVEGVQ-----VSLQELREAFERAVHRQLMTDVPYGVLL 237

Query: 261 SGGLDSSSIVCA----MRHLLPDGE------IKVFSLVFPGKEIDESKYQRIVVDECRVS 310
           SGGLDSS +        RH +   +       ++ S     K+  +     +  +     
Sbjct: 238 SGGLDSSLVAAVAARYARHRIETNDQSEAWWPRLHSFAIGLKDSPDLSAANVAAEALNTV 297

Query: 311 WYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLLDGQGSDE 367
            +   + +++ L D++  +    E +   +I     +  LAR     G+K++L G+GSDE
Sbjct: 298 HHGFEYTLQEGL-DVLPEVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDE 356

Query: 368 ILAGYHYF 375
           I  GY YF
Sbjct: 357 IFGGYLYF 364


>gb|AAB48058.1| (L40327) asparagine synthetase [Medicago sativa]
           Length = 586
           
 Score =  129 bits (320), Expect = 1e-28
 Identities = 112/391 (28%), Positives = 179/391 (45%), Gaps = 51/391 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  ++RK+L    + F +  D +VI   Y E+G + V+
Sbjct: 60  DQPLFNED--KSIIVTVNGEIYNHEDLRKQLPN--HKFRTQCDCDVIAHLYEEYGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  +SE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++    R+F  W               
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKDREFRRWYNPPWFNEAVIPTTP 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
            DP   ++ F +AV  RL+ DVP G  LSGGLDSS +       L   +       K+ S
Sbjct: 206 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGAKLHS 265

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
                K   + K  R V D      +   F ++D +  I D+I  T+    +T+ +    
Sbjct: 266 FCVGLKGAPDLKAGREVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325

Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           + + +  + +G+K ++ G+GSDEI  GY YF
Sbjct: 326 FLMSRKIKSSGVKWVISGEGSDEIFGGYLYF 356


>gb|AAB81011.1| (U89923) asparagine synthetase [Medicago sativa]
           Length = 586
           
 Score =  129 bits (320), Expect = 1e-28
 Identities = 112/391 (28%), Positives = 179/391 (45%), Gaps = 51/391 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  ++RK+L    + F +  D +VI   Y E+G + V+
Sbjct: 60  DQPLFNED--KSIIVTVNGEIYNHEDLRKQLPN--HKFRTQCDCDVIAHLYEEYGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  +SE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++    R+F  W               
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKDREFRRWYNPPWFNEAVIPTTP 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
            DP   ++ F +AV  RL+ DVP G  LSGGLDSS +       L   +       K+ S
Sbjct: 206 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGAKLHS 265

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
                K   + K  R V D      +   F ++D +  I D+I  T+    +T+ +    
Sbjct: 266 FCVGLKGAPDLKAGREVADFLGTVHHEFQFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325

Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           + + +  + +G+K ++ G+GSDEI  GY YF
Sbjct: 326 FLMSRKIKSSGVKWVISGEGSDEIFGGYLYF 356


>pir||T50812 asparagine synthetase ASN3 - Arabidopsis thaliana
           >gi|8953405|emb|CAB96680.1| (AL360334) asparagine
           synthetase ASN3 [Arabidopsis thaliana]
           Length = 578
           
 Score =  128 bits (319), Expect = 2e-28
 Identities = 117/392 (29%), Positives = 178/392 (44%), Gaps = 54/392 (13%)

Query: 1   MCGINGF------SWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI         S      +  ++  +RHRGPD  G++  ++  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPT-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K + +  NGEIYN   +R+ L  K + F + +D EVI   Y E G + V+
Sbjct: 60  DQPL-YNED-KTIAVTVNGEIYNHKALRENL--KSHQFRTGSDCEVIAHLYEEHGEEFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM+AFV+ D        +RD  GI PLY  +  DG ++ F+SE+K +          
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGITPLYIGWGLDG-SVWFASEMKAL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                          ++D   E      PG+  ++         W        +      
Sbjct: 165 ---------------SDDC--EQFMCFPPGH--IYSSKQGGLRRWYNPPWFSEVVPSTPY 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEI-----KVFS 286
            DP   ++ F +AV  RL+ DVP G  LSGGLDSS +   A+RHL           K+ +
Sbjct: 206 -DPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVALRHLEKSEAACQWGSKLHT 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                K   + K  R V D      +   F V+D + D I+ +    E +   +I     
Sbjct: 265 FCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI-DAIEEVIYHVETYDVTTIRASTP 323

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K++L G+GSDEI  GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355


>sp|P49094|ASNS_MAIZE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|7438077|pir||T02978
           asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           - maize >gi|984262|emb|CAA58052.1| (X82849) asparragine
           synthetase [Zea mays]
           Length = 586
           
 Score =  128 bits (319), Expect = 2e-28
 Identities = 117/387 (30%), Positives = 177/387 (45%), Gaps = 54/387 (13%)

Query: 3   GINGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEK 62
           G+   S      +  ++  +RHRGPD  G++  ++  L H RLAIID +  G QP+ Y +
Sbjct: 9   GVVEVSLAKRSRIIELSRRLRHRGPDWSGLHCHEDCYLAHQRLAIIDPT-SGDQPL-YNE 66

Query: 63  DGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAF 122
           D K V +  NGEIYN  E++ +L  K + F + +D EVI   Y E+G + V+  +GM++F
Sbjct: 67  D-KTVVVTVNGEIYNHEELKAKL--KTHEFQTGSDCEVIAHLYEEYGEEFVDMLDGMFSF 123

Query: 123 VIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGI---LQHNIPRKPNDAVIF 177
           V+ D        +RD  GI PLY  +  DG ++ FSSE+K +    +  I   P      
Sbjct: 124 VLLDTRDKSFIAARDAIGICPLYMGWGLDG-SVWFSSEMKALSDDCERFITFPPG----- 177

Query: 178 DFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQG 237
                    H   +   G++R    YN  +   T     +    LRE  EK         
Sbjct: 178 ---------HLYSSKTGGLRR---WYNPPWFSETVPSTPYNALFLREMFEK--------- 216

Query: 238 FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFSLVFPG 291
                  AV  RL+ DVP G  LSGGLDSS +       L + ++      K+ +     
Sbjct: 217 -------AVIKRLMTDVPFGVLLSGGLDSSLVASVASRHLNETKVDRQWGNKLHTFCIGL 269

Query: 292 KEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYGQYRVM 348
           K   + K  R V D      +   F V+   D L ++I  I T +      S    + + 
Sbjct: 270 KGSPDLKAAREVADYLSTVHHEFHFTVQEGIDALEEVIYHIETYDVTTIRAST-PMFLMS 328

Query: 349 KLARENGMKVLLDGQGSDEILAGYHYF 375
           +  +  G+K+++ G+GSDEI  GY YF
Sbjct: 329 RKIKSLGVKMVISGEGSDEIFGGYLYF 355


>sp|P49078|ASNS_ARATH ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|7438081|pir||T12989
           asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           - Arabidopsis thaliana >gi|507946|gb|AAA74359.1|
           (L29083) glutamine-dependent asparagine synthetase
           [Arabidopsis thaliana] >gi|5541701|emb|CAB51206.1|
           (AL096860) glutamine-dependent asparagine synthetase
           [Arabidopsis thaliana]
           Length = 584
           
 Score =  128 bits (319), Expect = 2e-28
 Identities = 119/392 (30%), Positives = 179/392 (45%), Gaps = 54/392 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  +RHRGPD  G+Y + +  L H RLA+ID +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+RK L  K + F + +D EVI   Y E+G D V+
Sbjct: 60  DQPLFNED--KTIVVTVNGEIYNHEELRKRL--KNHKFRTGSDCEVIAHLYEEYGVDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+   +      F+ W Y             
Sbjct: 166 DDC------------------EHFETFPPGH--FYSSKLGGFKQW-YNPPWFNESVPSTP 204

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VFS 286
            +P   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL      K     + S
Sbjct: 205 YEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKQWGPQLHS 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                +   + K  + V +      +   F+V+D +  I D+I    E +   +I     
Sbjct: 265 FCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGIDAIEDVI-YHVETYDVTTIRASTP 323

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K++L G+G+DEI  GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVLSGEGADEIFGGYLYF 355


>gb|AAF74755.1| (AF263432) asparagine synthetase [Helianthus annuus]
           Length = 589
           
 Score =  128 bits (318), Expect = 2e-28
 Identities = 124/393 (31%), Positives = 183/393 (46%), Gaps = 56/393 (14%)

Query: 1   MCGINGF-SWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G+Y   +  L H RLA+ID +  G
Sbjct: 1   MCGILALLGCSDDSQAKRFRVLELSRRLKHRGPDWSGLYQHGDNYLSHQRLAVIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D + V +  NGEIYN ME+R++L   G+ F + +D +VI   Y E G + V+
Sbjct: 60  DQPL-YNED-ETVVVTVNGEIYNHMELREKL--MGHKFKTGSDCDVIAHLYEEHGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM++FV+     N   ++RD  GI  LY  +  DG +++ SS +KG+         N
Sbjct: 116 MLDGMFSFVLLGTRDNSYLVARDAIGITSLYIGWGLDG-SVLISSGLKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D            +H E  F  G          ++    R F  W  Y+L    E     
Sbjct: 166 DD----------CEHFE-VFLAG---------HLYSSKIRGFRRW--YNLPWFSESIPST 203

Query: 233 S-DPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VF 285
             DP   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL      K     + 
Sbjct: 204 PYDPLVLRCAFENAVIKRLMTDVPFGVLLSGGLDSSLVASVTARHLAGSKAAKQWGAQLH 263

Query: 286 SLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQY 345
           S     +   + K  R V D      +   F V+D +  I D+I    E +   +I    
Sbjct: 264 SFCVGLEGSPDLKAAREVADYLGTLHHEFHFTVQDGIDAIEDVI-YHIETYDVTTIRAST 322

Query: 346 RVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
            +  ++R+    G+K+++ G+GSDEI  GY YF
Sbjct: 323 PMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355


>dbj|BAA96251.1| (AB035247) asparagine synthetase [Astragalus sinicus]
           Length = 584
           
 Score =  128 bits (318), Expect = 2e-28
 Identities = 118/392 (30%), Positives = 178/392 (45%), Gaps = 54/392 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D    KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCADASRAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K V +  NGEIYN  ++RK+L    +TF + +D +VI   Y E+G D V+
Sbjct: 60  DQPLFNED--KSVIVTVNGEIYNHEDLRKQLPN--HTFRTGSDCDVIAHLYEEYGEDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSYIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++      F  W Y  L          
Sbjct: 166 DDC------------------EHFECFPPGH--LYSSKNGGFRRW-YNPLWYSEAIPSAP 204

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFS 286
            DP   +  F +AV  RL+ DVP G  LSGGLDSS +       L   +       K+ S
Sbjct: 205 YDPIVLRQAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHS 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                +   + K  + V D      +  TF V+D +  I D+I    E +   +I     
Sbjct: 265 FCVGLEGSPDLKAGKEVADYLGTVHHEFTFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 323

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K ++ G+GSDEI  GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355


>gb|AAC16325.1| (AF061740) asparagine synthetase [Elaeagnus umbellata]
           Length = 585
           
 Score =  128 bits (318), Expect = 2e-28
 Identities = 122/392 (31%), Positives = 180/392 (45%), Gaps = 54/392 (13%)

Query: 1   MCGINGF-SWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAIID +  G
Sbjct: 1   MCGILALLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLFQFGDCYLAHQRLAIIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K V +  NGEIYN  E+RK+L    + F + +D +VI   Y E+G D V+
Sbjct: 60  DQPLFNED--KTVVVTVNGEIYNHEELRKKLSH--HKFRTGSDCDVIAHLYEEYGEDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G++AFV+ D   N   ++RD  G+  LY  +  DG +I  SSE+KG+         N
Sbjct: 116 MLDGIFAFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SIWISSELKGL--------NN 166

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D   F+        H   +   G++R    YN V+   T     +               
Sbjct: 167 DCEHFETFPPG---HLYSSKAGGMRR---WYNPVWFSETIPSAPY--------------- 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHL-----LPDGEIKVFS 286
            DP   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL           K+ S
Sbjct: 206 -DPLALRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLSGTKAAKQWGTKLHS 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                +   + K  + V        +   F V+D +  I D+I    E +   +I     
Sbjct: 265 FCVGLEGSPDLKAAKEVAAYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 323

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K+++ G+GSDEI  GY YF
Sbjct: 324 MFLMSRKIKSLGVKMVMSGEGSDEIFGGYLYF 355


>sp|P49093|ASN2_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2
           (GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE 2)
           >gi|2147132|pir||S57932 asparagine synthase
           (glutamine-hydrolysing) (EC 6.3.5.4) - Lotus japonicus
           >gi|2147134|pir||S69183 asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) 2 - Lotus japonicus
           >gi|897773|emb|CAA61590.1| (X89410) asparagine synthase
           (glutamine-hydrolysing) [Lotus japonicus]
           Length = 586
           
 Score =  128 bits (318), Expect = 2e-28
 Identities = 116/394 (29%), Positives = 182/394 (45%), Gaps = 58/394 (14%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDNFLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   + + +  NGEI+N  E+RK+L    + F +  D +VI   Y E G + V+
Sbjct: 60  DQPLFNED--QSIIVTVNGEIFNHEELRKQLPN--HKFRTGCDCDVIAHLYEEHGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  Y  DG ++  +SE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDG-SVWIASELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
           D    D  ++ L                PG+  ++    ++F  W  Y+     E I   
Sbjct: 166 D----DCEHFELFP--------------PGH--LYSSKEKEFRRW--YNPPWFSEAIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
               DP   +  F +A+  RL+ DVP G  LSGGLDSS +       L D +       K
Sbjct: 204 P--YDPLALRQAFEKAIIKRLMTDVPFGVLLSGGLDSSLVASVTARYLADTKAAKQWGSK 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
           + S     +   + K  R V D      +   + ++D +  I D+I   E    T    G
Sbjct: 262 LHSFCVGLEGAPDLKAAREVADYIGTVHHEFQYTIQDGIDAIEDVIYHVETYDVTTIRAG 321

Query: 344 --QYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
              + + +  +  G+K++L G+GSDEI AGY YF
Sbjct: 322 TPMFLMSRKIKSLGVKMVLSGEGSDEIFAGYLYF 355


>sp|Q43011|ASNS_ORYSA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|7438076|pir||T03602 probable
           asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           - rice >gi|1432054|gb|AAB03991.1| (U55873) asparagine
           synthetase [Oryza sativa] >gi|1902992|dbj|BAA18951.1|
           (D83378) asparagine synthetase [Oryza sativa]
           Length = 591
           
 Score =  127 bits (316), Expect = 4e-28
 Identities = 117/395 (29%), Positives = 182/395 (45%), Gaps = 60/395 (15%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D  L KR     ++  +RHRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGVADVSLAKRSRIIELSRRLRHRGPDWSGIHCYQDCYLAHQRLAIVDPT-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K V +  NGEIYN  E++  L  K + F + +D EVI   Y E+G + V+
Sbjct: 60  DQPL-YNED-KSVVVTVNGEIYNHEELKANL--KSHKFQTASDCEVIAHLYEEYGEEFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGI---LQHNIPR 169
             +GM+AFV+ D        +RD  GI PLY  +  DG ++ FSSE+K +    +  I  
Sbjct: 116 MLDGMFAFVLLDTRDKSFIAARDAIGICPLYMGWGLDG-SVWFSSEMKALSDDCERFISF 174

Query: 170 KPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKR 229
            P               H   +   G++R    YN  +   +     +    LR+  EK 
Sbjct: 175 PPG--------------HLYSSKTGGLRR---WYNPPWFSESIPSTPYNPLLLRQSFEK- 216

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
                          A+  RL+ DVP G  LSGGLDSS +   +   L + ++      K
Sbjct: 217 ---------------AIIKRLMTDVPFGVLLSGGLDSSLVASVVSRHLAEAKVAAQWGNK 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
           + +     K   + +  + V D      +   F V++ + D ++ +    E +   +I  
Sbjct: 262 LHTFCIGLKGSPDLRAAKEVADYLGTVHHELHFTVQEGI-DALEEVIYHVETYDVTTIRA 320

Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
              +  ++R+    G+K++L G+GSDEI  GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 355


>sp|P31752|ASNS_ASPOF ASPARAGINE SYNTHASE [GLUTAMINE-HYDROLYZING] (AS)
           >gi|100980|pir||S25165 asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) - garden asparagus
           >gi|16076|emb|CAA48141.1| (X67958) asparagine synthase
           (glutamine-hydrolysing) [Asparagus officinalis]
           Length = 590
           
 Score =  127 bits (315), Expect = 5e-28
 Identities = 125/397 (31%), Positives = 184/397 (45%), Gaps = 64/397 (16%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G+    +  L H RLAIID +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K + +  NGEIYN  E+R+ L +  Y   + +D EVI   Y E G D V+
Sbjct: 60  DQPL-YNED-KSIVVTVNGEIYNHEELRRRLPDHKYR--TGSDCEVIAHLYEEHGEDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM++FV+ D   N    +RD  GI PLY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDG-SVWLSSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
           D            +H E         + P  N ++   +  F  W   ++Y+  E I   
Sbjct: 166 DD----------CEHFE---------VFPPGN-LYSSRSGSFRRWYNPQWYN--ETIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GE 281
               DP   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL           +
Sbjct: 204 P--YDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQ 261

Query: 282 IKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSI 341
           +  F +   G    + K  + V +      +   F V+D +  I D+I    E +   +I
Sbjct: 262 LHSFCVGLEGS--PDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-FHIETYDVTTI 318

Query: 342 YGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
                +  +AR+    G+K+++ G+GSDEI  GY YF
Sbjct: 319 RASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYF 355


>emb|CAA67889.1| (X99552) asparagine synthetase [Asparagus officinalis]
           Length = 590
           
 Score =  127 bits (315), Expect = 5e-28
 Identities = 125/397 (31%), Positives = 184/397 (45%), Gaps = 64/397 (16%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G+    +  L H RLAIID +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K + +  NGEIYN  E+R+ L +  Y   + +D EVI   Y E G D V+
Sbjct: 60  DQPL-YNED-KSIVVTVNGEIYNHEELRRRLPDHKYR--TGSDCEVIAHLYEEHGEDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM++FV+ D   N    +RD  GI PLY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDG-SVWLSSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
           D            +H E         + P  N ++   +  F  W   ++Y+  E I   
Sbjct: 166 DD----------CEHFE---------VFPPGN-LYSSRSGSFRRWYNPQWYN--ETIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPD-------GE 281
               DP   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL           +
Sbjct: 204 P--YDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQ 261

Query: 282 IKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSI 341
           +  F +   G    + K  + V +      +   F V+D +  I D+I    E +   +I
Sbjct: 262 LHSFCVGLEGS--PDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-FHIETYDVTTI 318

Query: 342 YGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
                +  +AR+    G+K+++ G+GSDEI  GY YF
Sbjct: 319 RASTPMFLMARKIKSLGVKMVISGEGSDEIFGGYLYF 355


>sp|P19251|ASN1_PEA ASPARAGINE SYNTHETASE, NODULE [GLUTAMINE-HYDROLYZING]
           (GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE)
           >gi|68661|pir||AJPMN1 asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) 1 - garden pea
           >gi|20650|emb|CAA36429.1| (X52179) asparagine synthase
           (glutamine-hydrolysing) [Pisum sativum]
           Length = 586
           
 Score =  127 bits (315), Expect = 5e-28
 Identities = 113/391 (28%), Positives = 179/391 (44%), Gaps = 51/391 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+RK+L    + F +  D +VI   Y E G + V+
Sbjct: 60  DQPLFNED--KSIIVTVNGEIYNHEELRKQLPN--HKFFTQCDCDVIAHLYEEHGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  +SE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWIASELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++    R+F  W               
Sbjct: 166 DEC------------------EHFEVFPPGH--LYSSKEREFRRWYNPPWFNEAIIPSTP 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIKVFSLVFPG 291
            DP   ++ F +AV  RL+ DVP G  LSGGLDSS +     R+L      K +    P 
Sbjct: 206 YDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLAGTKAAKQWGAKLPS 265

Query: 292 -----KEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLI-RTQEEPFSTL-SIYGQ 344
                K   + K  + V D      +   F ++D +  I D+I  T+    +T+ +    
Sbjct: 266 FCVGLKGAPDLKAGKEVADFLGTVHHEFEFTIQDGIDAIEDVIYHTETYDVTTIRAATPM 325

Query: 345 YRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           + + +  + +G+K ++ G+GSDEI  GY YF
Sbjct: 326 FLMSRKIKSSGVKWVISGEGSDEIFGGYLYF 356


>sp|P49092|ASN1_LOTJA ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 1
           (GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE 1)
           >gi|2147131|pir||S57931 asparagine synthase
           (glutamine-hydrolysing) (EC 6.3.5.4) - Lotus japonicus
           >gi|2147133|pir||S69182 asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) 1 - Lotus japonicus
           >gi|897771|emb|CAA61589.1| (X89409) asparagine synthase
           (glutamine-hydrolysing) [Lotus japonicus]
           Length = 586
           
 Score =  126 bits (314), Expect = 6e-28
 Identities = 113/392 (28%), Positives = 177/392 (44%), Gaps = 54/392 (13%)

Query: 1   MCGI------NGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI      + F+      V  ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+RK+L    + F + +D +VI   Y E G + ++
Sbjct: 60  DQPLFNED--KSIIVTVNGEIYNHEELRKQLPN--HQFRTGSDCDVIAHLYEEHGENFMD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++    R F  W Y             
Sbjct: 166 DDC------------------EHFEVFPPGH--LYSSRERAFRRW-YNPTWFSESIPSAP 204

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFS 286
            DP   +  F +AV  RL+ DVP G  LSGGLDSS +       L   +       K+ S
Sbjct: 205 YDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHS 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                +   + K  + V D      +  TF V+D + D I+ +    E +   +I     
Sbjct: 265 FCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGI-DAIEEVIYHVETYDVTTIRASTP 323

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K ++ G+GSDEI  GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355


>pir||JQ2245 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - garden
           asparagus
           Length = 590
           
 Score =  125 bits (312), Expect = 1e-27
 Identities = 151/536 (28%), Positives = 224/536 (41%), Gaps = 121/536 (22%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G+    +  L H RLAIID +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K + +  NGEIYN  E+R+ L +  Y   + +D EVI   Y E G D V+
Sbjct: 60  DQPL-YNED-KSIVVTVNGEIYNHEELRRRLPDHKYR--TGSDCEVIAHLYEEHGEDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +GM++FV+ D   N    +RD  GI PLY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDG-SVWLSSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAW---KYYDLRERIEKR 229
           D            +H E         + P  N ++   +  F  W   ++Y+  E I   
Sbjct: 166 DD----------CEHFE---------VFPPGN-LYSSRSGSFRRWYNPQWYN--ETIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC-AMRHLLPDGEIKVFSLV 288
               DP   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL            
Sbjct: 204 P--YDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHL------------ 249

Query: 289 FPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVM 348
             G +  E              W     +    L    DL   +E             + 
Sbjct: 250 -AGSKAAE-------------QWGTQLHSFCVGLEGSPDLKAAKE------------LMA 283

Query: 349 KLARENGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVP 408
           +  +  G+K+++ G+GSDEI  GY YF      E        K+       K +H     
Sbjct: 284 RKIKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHHETCRKI-------KALHQYDCL 336

Query: 409 LKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYS 468
             N+  Y+  V          F +  ++ I+       +++I+ I+T +   + A T   
Sbjct: 337 RANVAEYLGTV-------HHEFHFTVQDGIDAI-----EDVIFHIETYDVTTIRASTPMF 384

Query: 469 LPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKV----SAGITKVLLRK 520
                    K    W +E+RVPF D E ++  +S+ PESK+       I K +LRK
Sbjct: 385 ---------KATSAWGLEARVPFLDKEFMDVAMSIDPESKMIKPDLGRIEKWVLRK 431


>sp|Q61024|ASNS_MOUSE ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|1147605|gb|AAA85125.1|
           (U38940) asparagine synthetase [Mus musculus]
           Length = 561
           
 Score =  125 bits (312), Expect = 1e-27
 Identities = 161/636 (25%), Positives = 259/636 (40%), Gaps = 140/636 (22%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI      D+ L  +   A++  HRGPD    E V    N   G  RLA++D    G 
Sbjct: 1   MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
           QP++  K    +W+ YNGEIYN   +++  E   + + +N D E+IL  Y + G +  + 
Sbjct: 60  QPIRVRKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEKTIC 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGI--LQHN----- 166
             +G++AF++ D +   +FL RD +G++PL+        +   SE KG+  L+H+     
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFL 175

Query: 167 ------------IPRKPNDAV--IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR 212
                       +  KPN  V  +    Y++  D      ++ +++L PG    FDL T 
Sbjct: 176 KVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDSVEKLFPG----FDLETV 231

Query: 213 KFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA 272
           K                         + LF  A+K RL+ D  +G  LSGGLDSS +  +
Sbjct: 232 K----------------------NNLRILFDNAIKKRLMTDRRIGCLLSGGLDSSLVAAS 269

Query: 273 MRHLLPDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIID 327
           +   L + +++  + +     ++  +    R V +      +   FN E+    L ++I 
Sbjct: 270 LLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIF 329

Query: 328 LIRTQEEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRK 386
            + T +      S+ G Y + K  R+N    V+  G+GSDE+  GY YF      E   +
Sbjct: 330 SLETYDITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE 388

Query: 387 FKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPS 446
            +  +LL+E+  +  +        + +               R P+L   F   +   P 
Sbjct: 389 -ESERLLKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPP 434

Query: 447 KELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPE 506
              I   K   E  LL ET         FED N +   I  R                P+
Sbjct: 435 DMRI--PKNGIEKHLLRET---------FEDCNLLPKEILWR----------------PK 467

Query: 507 SKVSAGITKV------LLRKGLKGILPDEIRNRAS-KVGFATPDKDILKTNEGQKFALAV 559
              S GIT V      +L+  ++  + DE+ + AS K  F TP     KT EG       
Sbjct: 468 EAFSDGITSVKNSWFKILQDYVEHQVDDEMMSAASQKFPFNTP-----KTKEG------- 515

Query: 560 IDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQELW 595
                      + YR++   FE H  G+ +W    W
Sbjct: 516 -----------YFYRQI---FERHYPGRADWLTHYW 537


>sp|O24338|ASNS_SANAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|2245622|gb|AAB71532.1|
           (AF005724) asparagine synthetase [Sandersonia
           aurantiaca]
           Length = 525
           
 Score =  125 bits (311), Expect = 1e-27
 Identities = 119/393 (30%), Positives = 179/393 (45%), Gaps = 56/393 (14%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  ++HRGPD  G+    +  L H RLAIID +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLDHHGDCYLAHQRLAIIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ Y +D K + +  NGEIYN  E+RK L   G+TF + +D EVI   Y E G   + 
Sbjct: 60  DQPL-YNED-KTIIVTVNGEIYNHEELRKGLP--GHTFRTGSDCEVIAHLYEEHGESFIH 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N    +RD  G+ PLY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGIFSFVLLDSRNNSFVAARDAIGVTPLYIGWGLDG-SVWISSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  K   PG+  ++      F+ W  Y+     E    +
Sbjct: 166 DDC------------------EHFKFFPPGH--LYSSKEGSFKRW--YNPPWFSEVIPSV 203

Query: 233 S-DPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVF 285
             DP   +  F  AV  RL+ DVP G  LSGGLDSS +       L   +       ++ 
Sbjct: 204 PFDPLALRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLEGTKAAELWGTQLH 263

Query: 286 SLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQY 345
           S     +   + K  + V +      +   F V+D +  I D+I    E +   +I    
Sbjct: 264 SFCVGLEGSPDLKAAKEVANFLGTIHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRAST 322

Query: 346 RVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
            +  ++R+    G+K+++ G+GSDEI  GY YF
Sbjct: 323 PMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYF 355


>prf||2207183A Asn synthetase [Rattus norvegicus]
           Length = 561
           
 Score =  125 bits (310), Expect = 2e-27
 Identities = 156/623 (25%), Positives = 254/623 (40%), Gaps = 114/623 (18%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI      D+ L  +   A++  HRGPD    E V    N   G  RLA++D    G 
Sbjct: 1   MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
           QP++  K    +W+ YNGEIYN   +++  E   + + +N D E+IL  Y + G +  + 
Sbjct: 60  QPIRVRKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEKTIC 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
             +G++AF++ D +   +FL RD +G++PL+        +   SE KG++          
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKALTEDGFLAVCSEAKGLVS--------- 166

Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
                      L H+   F + ++  +PG+  V DL         E  KY          
Sbjct: 167 -----------LKHSTTPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHA 214

Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
            YD  E++    EI   +   + LF  A+K RL+ D  +G  LSGGLDSS +  ++   L
Sbjct: 215 IYDSVEKLFPGFEIETVKNNLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQL 274

Query: 278 PDGEIKVFSLVFP--GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQE-- 333
            + ++      F    ++  +    R V +      +   FN E+ +  + ++I + E  
Sbjct: 275 KEAQVPYALQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFSLETY 334

Query: 334 EPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKL 392
           +  +  +  G Y + K  R+N    V+  G+GSDE+  GY YF      E   + +  +L
Sbjct: 335 DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESERL 393

Query: 393 LREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWK 452
           L+E+  +  +        + +               R P+L   F   +   P +  I K
Sbjct: 394 LKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPPEMRIPK 440

Query: 453 IKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAG 512
                E  LL ET         FED N +   I  R                P+   S G
Sbjct: 441 DGI--EKHLLRET---------FEDSNLLPKEILWR----------------PKEAFSDG 473

Query: 513 ITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWD 572
           IT V  +     IL D + ++           D + +   QKF      +    K  Y+ 
Sbjct: 474 ITSV--KNSWFKILQDFVEHQVD---------DAMMSEASQKFPFNTPQT----KEGYY- 517

Query: 573 YRKVLRMFEEHVSGKKNWSQELW 595
           YR++   FE H  G+ +W    W
Sbjct: 518 YRQI---FEHHYPGRADWLTHYW 537


>pir||JW0071 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - soybean
           >gi|1305549|gb|AAC09952.1| (U55874) asparagine
           synthetase [Glycine max]
           Length = 579
           
 Score =  124 bits (308), Expect = 3e-27
 Identities = 117/395 (29%), Positives = 175/395 (43%), Gaps = 60/395 (15%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D    KR     ++  ++HRGPD  G++   +  L H RLAI+D    G
Sbjct: 1   MCGILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGDNYLAHQRLAIVD-PASG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K V +  NGEIYN  E+RK+L    +TF + +D +VI   Y E G + V+
Sbjct: 60  DQPLFNE--DKTVVVTVNGEIYNHEELRKQL--PNHTFRTGSDCDVIAHLYEEHGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++F + D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGIFSFALLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
           D                    E  +   PG+  ++    R F  W  Y+     E I   
Sbjct: 166 DDC------------------EHFESFPPGH--LYSSKERAFRRW--YNPPWFSEAIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IK 283
               DP   +  F +AV  RL+ DVP G  LSGGLDSS +       L           K
Sbjct: 204 P--YDPLALRHAFEKAVVKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTNAAKQWGTK 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLS 340
           + S     +   + K  + V D      +   + V+   D + D+I  I T +      S
Sbjct: 262 LHSFCVGLEGAPDLKAAKEVADYIGTVHHEFHYTVQDGIDAIEDVIYHIETYDVTTIRAS 321

Query: 341 IYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           I   + + +  +  G+K ++ G+GSDEI  GY YF
Sbjct: 322 I-PMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355


>pir||T39308 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) SPBC119.10
           [similarity] - fission yeast (Schizosaccharomyces pombe)
           >gi|2959371|emb|CAA17925.1| (AL022117) asparagine
           synthetase [Schizosaccharomyces pombe]
           Length = 557
           
 Score =  124 bits (308), Expect = 3e-27
 Identities = 122/407 (29%), Positives = 185/407 (44%), Gaps = 71/407 (17%)

Query: 1   MCGINGFSWGDEEL------VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       E++         ++  +RHRGPD  G  + +   L H RLAI+ +   G
Sbjct: 1   MCGILAVHHVAEDIEAFKPKALHLSKQLRHRGPDWSGKAIRNQTILCHERLAIVGVE-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  + DGK V  V NGEIYN +++R+ L+   Y F + +D EVIL  Y E G  C  
Sbjct: 60  AQPLVSD-DGKLVLTV-NGEIYNHLKLRENLKGN-YKFKTYSDCEVILYLYREHGPACAN 116

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKN---IIFSSEIKGILQHNIPRKP 171
             +GM+++V+YD+ K+ +  +RD  GI  LY  +   +     F+SE+K +  H +  K 
Sbjct: 117 MLDGMFSWVLYDQDKDKVVAARDPIGITTLYQGFSSDSPDTAYFASELKAL--HPVCDK- 173

Query: 172 NDAVIFDF---LYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEK 228
               I  F    YY+    T+ T    ++   P +   +D N        Y  LRE +E 
Sbjct: 174 ----IIAFPPGHYYD--SETKQT----VRYFKPSW---WDENKIPSNPVDYKLLRETLE- 219

Query: 229 RKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVC----------------- 271
                           +V+ RL+A+VP G  LSGGLDSS I                   
Sbjct: 220 ---------------ASVRKRLMAEVPYGVLLSGGLDSSLIASIAARETEKLANSTSQSE 264

Query: 272 AMRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDL 328
             R +    ++  F++  PG    +    R V D      +  TF ++   D L D+I  
Sbjct: 265 EARTITAWPKLHSFAIGLPGS--PDLLAARKVADFLHTFHHEHTFTIDEGLDALRDVIYH 322

Query: 329 IRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           + T +      S    Y + +  +  G+K++L G+GSDEI  GY YF
Sbjct: 323 LETYDVTTIRAST-PMYLLSRKIKAQGVKMVLSGEGSDEIFGGYLYF 368


>sp|P19891|ASNS_CRIGR ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|68657|pir||AJHYNC asparagine
           synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - Chinese
           hamster >gi|191064|gb|AAA36977.1| (M27838) asparagine
           synthetase [Cricetulus longicaudatus]
           Length = 561
           
 Score =  123 bits (306), Expect = 5e-27
 Identities = 155/624 (24%), Positives = 256/624 (40%), Gaps = 116/624 (18%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI      D+ L  +   A++  HRGPD    E V    N   G  RLA++D    G 
Sbjct: 1   MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
           QP++ +K    +W+ YNGEIYN   +++  E   + + +N D E+IL  Y + G +  + 
Sbjct: 60  QPIRVKKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEQTIC 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
             +G++AF++ D +   +FL RD +G++PL+        +   SE KG++          
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVS--------- 166

Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKYYDLRER---- 225
                      L H+   F + ++  +PG+  V DL         E  KY+  R+     
Sbjct: 167 -----------LKHSTTPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHA 214

Query: 226 ----IEKRKEISDPQGFKD----LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
               +EK  +  + +  K     LF  AV+ RL+ D  +G  LSGGLDSS +  ++   L
Sbjct: 215 LYDSVEKLFQGFELETVKSNLRILFDSAVRKRLMTDRRIGCLLSGGLDSSLVAASLLKQL 274

Query: 278 PDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIIDLIRTQ 332
            + +++  + +     ++  +    R V +      +   FN E+    L ++I  + T 
Sbjct: 275 KEAQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIFSLETY 334

Query: 333 EEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGK 391
           +      S+ G Y + K  R+N    V+  G+GSDE+  GY YF      E   + +  +
Sbjct: 335 DITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESER 392

Query: 392 LLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIW 451
           LL+E+  +  +        + +               R P+L   F   +   P +  I 
Sbjct: 393 LLKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPPEMRI- 438

Query: 452 KIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSA 511
             K   E  LL ET         FED N +   I  R                P+   S 
Sbjct: 439 -PKNGIEKHLLRET---------FEDSNLLPKEILWR----------------PKEAFSD 472

Query: 512 GITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYW 571
           GIT V  +     IL D + ++           D +     QKF        + K +  +
Sbjct: 473 GITSV--KNSWFKILQDYVEHQVD---------DEMMATAAQKFPF-----NTPKTKEGY 516

Query: 572 DYRKVLRMFEEHVSGKKNWSQELW 595
            YR++   FE H  G+ +W    W
Sbjct: 517 YYRQI---FERHYPGRADWLTHYW 537


>sp|P49088|ASNS_RAT ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|1083600|pir||S53447
           asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           [validated] - rat >gi|460631|gb|AAA77671.1| (U07201)
           asparagine synthetase [Rattus norvegicus]
           >gi|460633|gb|AAA77672.1| (U07202) asparagine synthetase
           [Rattus norvegicus]
           Length = 561
           
 Score =  123 bits (305), Expect = 7e-27
 Identities = 156/623 (25%), Positives = 252/623 (40%), Gaps = 114/623 (18%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI      D+ L  +   A++  HRGPD    E V    N   G  RLA++D    G 
Sbjct: 1   MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
           QP++  K    +W+ YNGEIYN   +++  E   + + +N D E+IL  Y + G +  + 
Sbjct: 60  QPIRVRKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEKTIC 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPND 173
             +G++AF++ D +   +FL RD +G++PL+        +   SE KG++          
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKALTEDGFLAVCSEAKGLVS--------- 166

Query: 174 AVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR----KFEAWKY---------- 219
                      L H+   F + ++  +PG+  V DL         E  KY          
Sbjct: 167 -----------LKHSTTPFLK-VEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHA 214

Query: 220 -YDLRERIEKRKEISDPQG-FKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL 277
            YD  E++    EI   +   + LF  A+K RL+ D  +G  LSGGLDSS +  ++   L
Sbjct: 215 IYDSVEKLFPGFEIETVKNNLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQL 274

Query: 278 PDGEIKVFSLVFP--GKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQE-- 333
            + ++      F    ++  +    R V +      +   FN E+ +  + ++I   E  
Sbjct: 275 KEAQVPYALQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFPLETY 334

Query: 334 EPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKL 392
           +  +  +  G Y + K  R+N    V+  G+GSDE+  GY YF      E   + +  +L
Sbjct: 335 DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESERL 393

Query: 393 LREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWK 452
           L+E+  +  +        + +               R P+L   F   +   P +  I K
Sbjct: 394 LKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPPEMRIPK 440

Query: 453 IKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAG 512
                E  LL ET         FED N +   I  R                P+   S G
Sbjct: 441 DGI--EKHLLRET---------FEDSNLLPKEILWR----------------PKEAFSDG 473

Query: 513 ITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWD 572
           IT V  +     IL D +  +           D + +   QKF      +    K  Y+ 
Sbjct: 474 ITSV--KNSWFKILQDFVEYQVD---------DAMMSEASQKFPFNTPQT----KEGYY- 517

Query: 573 YRKVLRMFEEHVSGKKNWSQELW 595
           YR++   FE H  G+ +W    W
Sbjct: 518 YRQI---FEHHYPGRADWLTHYW 537


>gb|AAB95197.1| (U84118) asparagine synthetase [Aedes aegypti]
           Length = 562
           
 Score =  123 bits (305), Expect = 7e-27
 Identities = 113/383 (29%), Positives = 187/383 (48%), Gaps = 59/383 (15%)

Query: 13  ELVKRMNDAIRHRGPDDEGVYVDDNVS--LGHVRLAIIDLSPKGHQPMKYEKDGKEVWIV 70
           EL  R +   RHRGPD  G+  D++    L   RL +I +   G QP    +DG +V +V
Sbjct: 31  ELAFRQSARQRHRGPDHTGLVADEDEGFVLVQERLCVIGVKT-GTQPF-LSQDG-QVLLV 87

Query: 71  YNGEIYNFM----EIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCV-ERFNGMWAFVIY 125
            NGEIYN++    EI   L+     +T  +D +VI+A Y ++G D + +   GM+AFV+Y
Sbjct: 88  ANGEIYNYLLMAQEINDALKAGENMYTPRSDCDVIIAYYEKFGVDALMKNIRGMFAFVLY 147

Query: 126 DKSKNILFLSRDRFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFD--FLYY 182
           DK    + ++RD  GI PLY   D + N+  +SE+K +++   P    +  IF    +YY
Sbjct: 148 DKKNGCILVARDPIGIVPLYSGKDAEGNLWIASEMKCLVE-TCP----EVAIFPPGHMYY 202

Query: 183 NLLDHTED-TFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDL 241
              +  +  ++FE         + ++++   K +        ER+ K  E          
Sbjct: 203 GSREKLKSVSYFES--------DWMYEIPQAKVDL-------ERLNKSLE---------- 237

Query: 242 FLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLL-----PDGEIKVFSL-VFPGKEID 295
              AV+  L  DVP+G+ LSGGLDSS I      ++     PD  +K +S+ +  G + +
Sbjct: 238 --DAVESHLQCDVPMGALLSGGLDSSLIASIATKIMKKRHGPDYRLKTYSVGLVRGPDFE 295

Query: 296 ESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYRVMKLAR--- 352
              + R+V D          F +++ L  I D +    E +   ++     ++ L+R   
Sbjct: 296 ---FARMVADYIGSDHTEVYFTIDEGLNYIRDAV-LHGETYDITTVRCTIPLLLLSRYIK 351

Query: 353 ENGMKVLLDGQGSDEILAGYHYF 375
             G+K++L G+G+DE+  GY YF
Sbjct: 352 SEGIKMVLSGEGADELFGGYLYF 374


>emb|CAB57292.1| (AJ133522) asparagine synthetase (type-I) [Phaseolus vulgaris]
           Length = 579
           
 Score =  123 bits (305), Expect = 7e-27
 Identities = 116/395 (29%), Positives = 177/395 (44%), Gaps = 60/395 (15%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D    KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGDCYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+RK+L    +TF + +D +VI   Y E G + V+
Sbjct: 60  DQPLFNED--KTIAVTVNGEIYNHEELRKQLPN--HTFRTGSDCDVIAHLYEEHGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+          
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSELKGL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
                            +   E  +   PG+  ++    R+F  W  Y+     E I   
Sbjct: 165 -----------------NGDCEHFESFPPGH--LYSSKEREFRRW--YNPPWFSEAIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IK 283
               DP   +  F +AV  RL+ DVP G  LSGGLDSS +       L           K
Sbjct: 204 P--YDPLALRHAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLEGTNAAKHWGTK 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLS 340
           + S     +   + K  R V D      +   ++V+   D + D+I  I T +      S
Sbjct: 262 LHSFCVGLEGAPDLKAAREVADYIGTVHHEFHYSVQDGIDAIEDVIYHIETYDVTTIRAS 321

Query: 341 IYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           I   + + +  +  G+K  + G+GSDEI  GY YF
Sbjct: 322 I-PMFLMSRKIKSLGVKWDICGEGSDEIYGGYLYF 355


>pir||AJHUN2 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) (version
           2) - human >gi|339987|gb|AAA36781.1| (M15798) ts11 cell
           cycle control protein [Homo sapiens]
           Length = 540
           
 Score =  122 bits (303), Expect = 1e-26
 Identities = 153/602 (25%), Positives = 245/602 (40%), Gaps = 116/602 (19%)

Query: 22  IRHRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNF 78
           I HRGPD    E V    N   G  RLA++D    G QP++ +K    +W+ YNGEIYN 
Sbjct: 3   IAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGMQPIRVKKY-PYLWLCYNGEIYNH 60

Query: 79  MEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVERFNGMWAFVIYDKSKNILFLSRD 137
            ++++  E   + + +  D E+IL  Y + G +  +   +G++AFV+ D +   +FL RD
Sbjct: 61  KKMQQHFE---FEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRD 117

Query: 138 RFGIKPLYYYYDGKNII-FSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGI 196
            +G++PL+        +   SE KG++                     L H+   F + +
Sbjct: 118 TYGVRPLFKAMTEDGFLAVCSEAKGLVT--------------------LKHSATPFLK-V 156

Query: 197 KRLMPGYNAVFDLNTR----KFEAWKY-----------YDLRERIEKRKEISDPQG-FKD 240
           +  +PG+  V DL         E  KY           YD  E++    EI   +   + 
Sbjct: 157 EPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDVPLHALYDNVEKLFPGFEIETVKNNLRI 216

Query: 241 LFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEIK--VFSLVFPGKEIDESK 298
           LF  AVK RL+ D  +G  LSGGLDSS +   +   L + +++  + +     ++  +  
Sbjct: 217 LFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLL 276

Query: 299 YQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIY----GQYRVMK-LARE 353
             R V D      Y   FN E+ +  + ++I + E    TL  +    G Y + K + R 
Sbjct: 277 AARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLE--LMTLQQFVLRIGMYLISKYIGRT 334

Query: 354 NGMKVLLDGQGSDEILAGYHYFFGYYFVELLRKFKWGKLLREILAYKRIHGSLVPLKNMV 413
               ++  G+GSDE+  GY YF      E   + +  +LLRE                  
Sbjct: 335 QIAWLIFSGEGSDELTQGYIYFHKAPSPEKAEE-ESERLLRE------------------ 375

Query: 414 LYILPVWVTKRLWRRRFPYLREEFIEKFKKRPSKELIWKIKTLNEALLLAETYYSLPHLL 473
           LY+  V    R        LR  F++                       +  Y SLP  +
Sbjct: 376 LYLFDVLRADRTTAAHGLELRVPFLDH--------------------RFSSYYLSLPPEM 415

Query: 474 RFEDKNAMRWSIESRVPFCDHELVEYVLSLPPESKVSAGITKVLLRKGLKGILPDEIRNR 533
           R   KN +   +  R  F D  L+   +   P+   S GIT V  +     IL + + ++
Sbjct: 416 RI-PKNGIEKHL-LRETFEDSNLIPKEILWRPKEAFSDGITSV--KNSWFKILQEYVEHQ 471

Query: 534 ASKVGFATPDKDILKTNEGQKFALAVIDSESFKKRPYWDYRKVLRMFEEHVSGKKNWSQE 593
                      D +  N  QKF        + K +  + YR+V   FE H  G+ +W   
Sbjct: 472 VD---------DAMMANAAQKFPF-----NTPKTKEGYYYRQV---FERHYPGRADWLSH 514

Query: 594 LW 595
            W
Sbjct: 515 YW 516


>sp|P19252|ASN2_PEA ASPARAGINE SYNTHETASE, ROOT [GLUTAMINE-HYDROLYZING]
           (GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE)
           >gi|68660|pir||AJPMN2 asparagine synthase
           (glutamine-hydrolyzing) (EC 6.3.5.4) 2 - garden pea
           >gi|20652|emb|CAA36430.1| (X52180) asparagine synthase
           (glutamine-hydrolysing) [Pisum sativum]
           Length = 583
           
 Score =  122 bits (302), Expect = 2e-26
 Identities = 115/392 (29%), Positives = 181/392 (45%), Gaps = 54/392 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D    KR     ++  ++HRGP+  G++   +  L   RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDPSRAKRVRVLELSRRLKHRGPEWSGLHQHGDCYLAQQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+ + +D   + +  NGEIYN  ++RK+L    +TF + +D +VI   Y E+G D V+
Sbjct: 60  DQPL-FNEDNPSI-VTVNGEIYNHEDLRKQLSN--HTFRTGSDCDVIAHLYEEYGEDFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV  D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         +
Sbjct: 116 MLDGIFSFVPLDTRDNSYIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL--------ND 166

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D   F+      L  ++D+ F                  R++    +Y   E I      
Sbjct: 167 DCEHFECFPPGHLYSSKDSGF------------------RRWYNPSWYS--EAIPSAP-- 204

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------KVFS 286
            DP   +  F +AV  RL+ DVP G  LSGGLDSS +       L   +       K+ S
Sbjct: 205 YDPLALRHAFEKAVVKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHS 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                +   + K  + V D      +  TF V+D +  I D+I    E +   SI     
Sbjct: 265 FCVGLEGSPDLKAGKEVADYLGTVHHEFTFTVQDGIDAIEDVI-YHVETYDVTSIRASTP 323

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K ++ G+GSDEI  GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355


>dbj|BAB17726.1| (AB050900) asparagine synthetase [Raphanus sativus]
           Length = 585
           
 Score =  121 bits (301), Expect = 2e-26
 Identities = 118/392 (30%), Positives = 175/392 (44%), Gaps = 53/392 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  +RHRGPD  G+Y +    L H RLAIID +  G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGIYQNGGNYLAHQRLAIIDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+RK L  K + F + +D +VI   Y E G + V+
Sbjct: 60  DQPLFNED--KSIVVTVNGEIYNHEELRKGL--KNHKFHTGSDCDVIAHLYEEHGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+          
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAVGVTSLYIGWGLDG-SLWVSSEMKGL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                         H +   FE      P  +     +   F+ W Y             
Sbjct: 165 --------------HEDCEHFEA----FPPGHLYSSKSGGGFKQW-YNPPWFNESVPSTP 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VFS 286
            +P   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL      K     + S
Sbjct: 206 YEPLAIRRAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKRWGPQLHS 265

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                +   + K  + V +      +   F V+D +  I D+I    E +   +I     
Sbjct: 266 FCVGLEGSPDLKAGKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 324

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K++L G+GSDEI  GY YF
Sbjct: 325 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 356


>emb|CAA08913.1| (AJ009952) asparagine synthetase type II [Phaseolus vulgaris]
           Length = 584
           
 Score =  121 bits (300), Expect = 3e-26
 Identities = 118/395 (29%), Positives = 178/395 (44%), Gaps = 60/395 (15%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D    KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDHSRAKRVRVLELSRRLKHRGPDWSGLHQHGDCFLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K V +  NGEIYN  E+RK+L   G+ F + +D +VI   Y E G   V+
Sbjct: 60  DQPLFNED--KSVIVTVNGEIYNHEELRKQLP--GHKFRTGSDCDVIAHLYEEHGEGFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGIFSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSEMKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD---LRERIEKR 229
           D                    E  +   PG+  ++    R F  W  Y+     E I   
Sbjct: 166 DDC------------------EHFECFPPGH--LYSSKDRGFRRW--YNPPWFSEAIPSA 203

Query: 230 KEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGEI------K 283
               DP   +  F +AV  RL+  VP G  LSGGLDSS +       L + +       K
Sbjct: 204 P--YDPLVLRRAFEQAVIKRLMTGVPFGVLLSGGLDSSLVASITSRYLANTKAAEQWGSK 261

Query: 284 VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG 343
           + S     +   + K  + V D      +   F V+D +  I D+I    E +   +I  
Sbjct: 262 LHSFCVGLEGSPDLKAAKEVADYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRA 320

Query: 344 QYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
              +  ++R+    G+K ++ G+GSDEI  GY YF
Sbjct: 321 STPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYF 355


>sp|P49091|ASNS_BRAOL ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|1084354|pir||S52387
           asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           - wild cabbage >gi|669057|emb|CAA59138.1| (X84448)
           asparagine synthase (glutamine-hydrolysing) [Brassica
           oleracea]
           Length = 586
           
 Score =  121 bits (300), Expect = 3e-26
 Identities = 118/392 (30%), Positives = 174/392 (44%), Gaps = 53/392 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D+   KR     ++  +RHRGPD  G+Y +    L H RLAIID    G
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGIYQNGFNYLAHQRLAIID-PDSG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K + +  NGEIYN  E+RK L  K + F + +D +VI   Y E G + V+
Sbjct: 60  DQPLFNED--KSIVVTVNGEIYNHEELRKGL--KNHKFHTGSDCDVIAHLYEEHGENFVD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+++FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+          
Sbjct: 116 MLDGIFSFVLLDTRDNSFMVARDAVGVTSLYIGWGLDG-SLWVSSEMKGL---------- 164

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                         H +   FE      P  +     +   F+ W Y             
Sbjct: 165 --------------HEDCEHFEA----FPPGHLYSSKSGGGFKQW-YNPPWFNESVPSTP 205

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV-CAMRHLLPDGEIK-----VFS 286
            +P   +  F  AV  RL+ DVP G  LSGGLDSS +     RHL      K     + S
Sbjct: 206 YEPLAIRSAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKRWGPQLHS 265

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQYR 346
                +   + K  + V +      +   F V+D +  I D+I    E +   +I     
Sbjct: 266 FCVGLEGSPDLKAGKEVAEYLGTVHHEFHFTVQDGIDAIEDVI-YHVETYDVTTIRASTP 324

Query: 347 VMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           +  ++R+    G+K++L G+GSDEI  GY YF
Sbjct: 325 MFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYF 356


>sp|P17714|ASNS_MESAU ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE) >gi|68658|pir||AJHYNG asparagine
           synthase (glutamine-hydrolyzing) (EC 6.3.5.4) - golden
           hamster >gi|49614|emb|CAA36375.1| (X52130) asparagine
           synthetase (AA 1-561) [Mesocricetus auratus]
           Length = 561
           
 Score =  120 bits (297), Expect = 6e-26
 Identities = 153/629 (24%), Positives = 255/629 (40%), Gaps = 126/629 (20%)

Query: 1   MCGINGFSWGDEELVKRMNDAIR--HRGPDD---EGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI      D+ L  +   A++  HRGPD    E V    N   G  RLA++D    G 
Sbjct: 1   MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVD-PLFGM 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFD-CVE 114
           QP++ +K    +W+ YNGEIYN   +++  E   + + +N D E+IL  Y + G +  + 
Sbjct: 60  QPIRVKKY-PYLWLCYNGEIYNHKALQQRFE---FEYQTNVDGEIILHLYDKGGIEQTIC 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNII-FSSEIKGI--LQHN----- 166
             +G++AF++ D +   +FL RD +G++PL+        +   SE KG+  L+H+     
Sbjct: 116 MLDGVFAFILLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFL 175

Query: 167 ------------IPRKPNDAV--IFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTR 212
                       +  KPN  V  +    Y++  D      ++ +++L PG    F+L T 
Sbjct: 176 KVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHALYDSVEKLFPG----FELETV 231

Query: 213 KFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA 272
           K                         + LF  AV+ RL+ D  +   LSGGLDSS +  +
Sbjct: 232 K----------------------SNLRILFDNAVRKRLMTDRRIVCLLSGGLDSSLVASS 269

Query: 273 MRHLLPDGEIK--VFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVED---VLADIID 327
           +   L + +++  + +     ++  +    R V +      +   FN E+    L ++I 
Sbjct: 270 LLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIF 329

Query: 328 LIRTQEEPFSTLSIYGQYRVMKLAREN-GMKVLLDGQGSDEILAGYHYFFGYYFVELLRK 386
            + T +      S+ G Y + K  R+N    V+  G+GSDE+  GY YF      E   +
Sbjct: 330 SLETYDITTVRASV-GMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEE 388

Query: 387 FKWGKLLREILAYKRIHGSLVPLKNMVLYILPVWVTKRLWRRRFPYLREEFIEKFKKRPS 446
            +  +LL+E+  +  +        + +               R P+L   F   +   P 
Sbjct: 389 -ESERLLKELYLFDVLRADRTTAAHGL-------------ELRVPFLDHRFSSYYLSLPP 434

Query: 447 KELIWKIKTLNEALLLAETYYSLPHLLRFEDKNAMRWSIESRVPFCDHELVEYVLSLPPE 506
           +  +   K   E  LL ET         FED N +   I  R                P+
Sbjct: 435 EMRV--PKNGIEKHLLRET---------FEDSNLLPKEILWR----------------PK 467

Query: 507 SKVSAGITKVLLRKGLKGILPDEIRNRASKVGFATPDKDILKTNEGQKFALAVIDSESFK 566
              S GIT V  +     IL D + ++           D +     QKF        + K
Sbjct: 468 EAFSDGITSV--KNSWFKILQDYVEHQVD---------DAMMATAAQKFPF-----NTPK 511

Query: 567 KRPYWDYRKVLRMFEEHVSGKKNWSQELW 595
            +  + YR++   FE H  G+ +W    W
Sbjct: 512 TKEGYFYRQI---FEHHYPGRADWLTHYW 537


>gi|6325403 asparagine synthetase; Asn1p [Saccharomyces cerevisiae]
           >gi|1351984|sp|P49089|ASN1_YEAST ASPARAGINE SYNTHETASE
           [GLUTAMINE-HYDROLYZING] 1 (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE 1) >gi|1077018|pir||S52694
           asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           ASN1 - yeast (Saccharomyces cerevisiae)
           >gi|747902|emb|CAA88594.1| (Z48675) asparagine
           synthetase [Saccharomyces cerevisiae]
           >gi|1066479|gb|AAB68284.1| (U40829) Similar to
           Asparagine synthetase B from E. coli (Swiss Prot.
           accession number P22106) and other species
           [Saccharomyces cerevisiae]
           Length = 572
           
 Score =  113 bits (280), Expect = 6e-24
 Identities = 116/415 (27%), Positives = 179/415 (42%), Gaps = 75/415 (18%)

Query: 1   MCGI-NGFSWGDEELVK----RMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI   F   D    K    +++  IRHRGPD  G  + ++    H RLAI+ +   G 
Sbjct: 1   MCGIFAAFRHEDVHRYKPKALQLSKRIRHRGPDWSGNAIKNSTIFVHERLAIVGVE-SGA 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVER 115
           QP+    DG E  +  NGEIYN +++R+E  +  Y F + +D E I+  YL+   D  + 
Sbjct: 60  QPIT-SSDG-EYMLCVNGEIYNHIQLREECAD--YEFGTLSDCEPIIPMYLKHDIDAPKY 115

Query: 116 FNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDG---KNIIFSSEIKGILQHNIPRKPN 172
            +GM+A+ +YD  ++ +  +RD  GI  LY        K + F+SE+K +          
Sbjct: 116 LDGMFAWTLYDAKQDRIVAARDPIGITTLYMGRSSASPKTVYFASELKCL---------- 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                          T+D   + I    PG+  V+D  T K   +   D  +        
Sbjct: 166 ---------------TDDC--DTITAFPPGH--VYDSKTDKITRYFTPDWLDEKRIPSTP 206

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIV--------------------CA 272
            D    +    +AV+ RL+A+VP G  LSGGLDSS I                       
Sbjct: 207 IDYMAIRHSLEKAVRKRLMAEVPYGVLLSGGLDSSLIASIAARETAKATNDVEPSTYDSK 266

Query: 273 MRHLL---PDGEI------KVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLA 323
            RHL     DG++       + S         + +  R V        +  TF +++ L 
Sbjct: 267 ARHLAGIDDDGKLHTAGWTSLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGL- 325

Query: 324 DIIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
           D +D +    E +   +I     +  L+R+    G+K++L G+GSDEI  GY YF
Sbjct: 326 DALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYF 380


>gi|6321563 asparagine synthetase; Asn2p [Saccharomyces cerevisiae]
           >gi|1351986|sp|P49090|ASN2_YEAST ASPARAGINE SYNTHETASE
           [GLUTAMINE-HYDROLYZING] 2 (GLUTAMINE-DEPENDENT
           ASPARAGINE SYNTHETASE 2) >gi|1084549|pir||S55982
           asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4)
           ASN2 - yeast (Saccharomyces cerevisiae)
           >gi|642347|emb|CAA58159.1| (X83099) glutamic-dependent
           asparagine synthase [Saccharomyces cerevisiae]
           >gi|1323203|emb|CAA97135.1| (Z72909) ORF YGR124w
           [Saccharomyces cerevisiae]
           Length = 572
           
 Score =  113 bits (280), Expect = 6e-24
 Identities = 114/414 (27%), Positives = 173/414 (41%), Gaps = 74/414 (17%)

Query: 1   MCGI-NGFSWGDEELVK----RMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGH 55
           MCGI   F   D    K    +++  IRHRGPD  G  V ++    H RLAI+ L   G 
Sbjct: 1   MCGIFAAFKHEDIHNFKPKALQLSKKIRHRGPDWSGNAVMNSTIFVHERLAIVGLD-SGA 59

Query: 56  QPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVER 115
           QP+    DG E  +  NGEIYN +++R+   +  Y F + +D E I+  YLE   D  + 
Sbjct: 60  QPIT-SADG-EYMLGVNGEIYNHIQLREMCSD--YKFQTFSDCEPIIPLYLEHDIDAPKY 115

Query: 116 FNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKN---IIFSSEIKGILQHNIPRKPN 172
            +GM+AF +YD  K+ +  +RD  G+  LY     ++   + F+SE+K +          
Sbjct: 116 LDGMFAFCLYDSKKDRIVAARDPIGVVTLYMGRSSQSPETVYFASELKCLTD-------- 167

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
                                + I    PG+  V+D  T K   +   D  +        
Sbjct: 168 -------------------VCDSIISFPPGH--VYDSETDKITRYFTPDWLDEKRIPSTP 206

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCA-------------------- 272
            D    +    +AV+ RL+A+VP G  LSGGLDSS I                       
Sbjct: 207 VDYHAIRHSLEKAVRKRLMAEVPYGVLLSGGLDSSLIAAIAARETEKANADANEDNNVDE 266

Query: 273 --------MRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLAD 324
                     HL   G  ++ S         + +  R V        +  TF +++ L D
Sbjct: 267 KQLAGIDDQGHLHTSGWSRLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGL-D 325

Query: 325 IIDLIRTQEEPFSTLSIYGQYRVMKLARE---NGMKVLLDGQGSDEILAGYHYF 375
            +D +    E +   +I     +  L+R+    G+K++L G+GSDEI  GY YF
Sbjct: 326 ALDDVIYHLETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYF 379


>gb|AAC49614.1| (U77679) asparagine synthetase 1 [Glycine max]
           Length = 579
           
 Score =  113 bits (280), Expect = 6e-24
 Identities = 110/392 (28%), Positives = 171/392 (43%), Gaps = 54/392 (13%)

Query: 1   MCGING-FSWGDEELVKR-----MNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       D    KR     ++  ++HRGPD  G++   +  L H RLAI+D +  G
Sbjct: 1   MCGILAVLGCSDSSQAKRVRVLELSRRLKHRGPDWSGLHQYGDNYLAHQRLAIVDPA-SG 59

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  E   K V +  NGEIYN  E+RK+L    +TF + +D +VI   Y E G + ++
Sbjct: 60  DQPLFNED--KTVVVTVNGEIYNHEELRKQLPN--HTFRTGSDCDVIAHLYEEHGENFMD 115

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPN 172
             +G+ +FV+ D   N   ++RD  G+  LY  +  DG ++  SSE+KG+         N
Sbjct: 116 MLDGISSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDG-SVWISSELKGL---------N 165

Query: 173 DAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEI 232
           D                    E  +   PG+  ++    R F  W Y      +      
Sbjct: 166 DDC------------------EHFESFPPGH--LYSSKERAFRRW-YNPPWLSLAIPSAP 204

Query: 233 SDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAMRHLLPDGE------IKVFS 286
            DP   +  F +    RL+ DVP G  LSGGLDSS +       L   +       K+ S
Sbjct: 205 YDPLALRHAFEKLWIKRLMTDVPFGVLLSGGLDSSLVAAVTARYLAGTKAAKQWGTKLHS 264

Query: 287 LVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DVLADIIDLIRTQEEPFSTLSIYG 343
                +   + K  + V +      +   + V+   D + D+I  I T +      SI  
Sbjct: 265 FCVGLEGAPDLKATKEVAEYIGTVHHEFHYTVQDGIDAIEDVIYHIETYDVTTIRASI-P 323

Query: 344 QYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
            + + +  +  G+K ++ G+GSD    GY YF
Sbjct: 324 MFLMSRKIKSLGVKWVISGEGSDVFFGGYLYF 355


>pir||T18441 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) C0395w
           [similarity] - malaria parasite (Plasmodium falciparum)
           >gi|3649765|emb|CAB11114.1| (Z98547) predicted using
           hexExon; MAL3P3.14 (PFC0395w), Asparagine synthetase,
           len: 611 aa; Similarity to many asparagine synthetases.
           O.sativa asparagine synthetase (SW:ASNS_ORYSA) BLAST
           Score: 1003, sum P(2) = 5.5e-165; 65% identity in 290 aa
           overlap
           Length = 610
           
 Score =  113 bits (279), Expect = 8e-24
 Identities = 126/414 (30%), Positives = 181/414 (43%), Gaps = 94/414 (22%)

Query: 18  MNDAIRHRGPDDEGVYVDDNVS-----LGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYN 72
           ++  +RHRGPD  G+ V++N       L H RLAI+D+   GHQP+ Y+ D +EV +  N
Sbjct: 24  LSKILRHRGPDWNGIVVEENDDGTTNVLAHERLAIVDVL-SGHQPL-YD-DEEEVCLTIN 80

Query: 73  GEIYNFMEIRKELEEKGYT-FTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNI 131
           GEIYN +E+RK ++E+      S +D  VI   +  +        +G++A VI DK  N 
Sbjct: 81  GEIYNHLELRKLIKEENLNKLKSCSDCAVIPNLFKIYKEKIPSMLDGIFAGVISDKKNNT 140

Query: 132 LFLSRDRFGIKPLY--YYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTE 189
            F  RD  GI PLY  Y  DG +I FSSE K +  + I       VIF            
Sbjct: 141 FFAFRDPIGICPLYIGYAADG-SIWFSSEFKALKDNCI-----RYVIFP----------- 183

Query: 190 DTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYD-----LRERIEKRKEISDPQGFKDLFLR 244
                      PG+   +  N  K E  +YY+     L   I   K   D    +    +
Sbjct: 184 -----------PGH--YYKNNKNKGEFVRYYNPNWWSLNNSIPNNK--VDFNEIRIHLEK 228

Query: 245 AVKMRLVADVPVGSCLSGGLDSSSIVCAM-RHL----------LPDG------------- 280
           AV  RL+ DVP G  LSGGLDSS I   + +HL          + +G             
Sbjct: 229 AVIKRLMGDVPFGILLSGGLDSSIIAAILAKHLNILDNKNGKSIQNGNDKKSNNNGNESN 288

Query: 281 ----------------EIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVE---DV 321
                           +++ FS+   G    + K  + V +   +      F VE   D 
Sbjct: 289 NNNNNNNFNNNNSGPQKLRSFSIGLKGS--PDLKAAKEVAEYLGIEHTEFYFTVEEGIDS 346

Query: 322 LADIIDLIRTQEEPFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGYHYF 375
           L D+I  I T +      S    Y + +L + + +K++L G+GSDEI  GY YF
Sbjct: 347 LHDVIYHIETYDITTIRAST-PMYILSRLIKSSCVKMVLSGEGSDEIFGGYLYF 399


>pir||T42010 probable asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) -
           fission yeast (Schizosaccharomyces pombe) (fragment)
           >gi|1749410|dbj|BAA13764.1| (D89101) similar to
           Saccharomyces cerevisiae Asparagine
           synthetase(glutamine-hydrolyzing)2, SWISS-PROT Accession
           Number P49090 [Schizosaccharomyces pombe]
           Length = 559
           
 Score =  102 bits (253), Expect = 9e-21
 Identities = 93/281 (33%), Positives = 137/281 (48%), Gaps = 48/281 (17%)

Query: 1   MCGINGFSWGDEEL------VKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKG 54
           MCGI       E++         ++  +RHRGPD  G  + +   L H RLAI+ +   G
Sbjct: 3   MCGILAVHHVAEDIEAFKPKALHLSKQLRHRGPDWSGKAIRNQTILCHERLAIVGVE-SG 61

Query: 55  HQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVE 114
            QP+  + DGK V  V NGEIYN +++R+ L+   Y F + +D EVIL  Y E G  C  
Sbjct: 62  AQPLVSD-DGKLVLTV-NGEIYNHLKLRENLKGN-YKFKTYSDCEVILYLYREHGPACAN 118

Query: 115 RFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKN---IIFSSEIKGILQHNIPRKP 171
             +GM+++V+YD+ K+ +  +RD  GI  LY  +   +     F+SE+K +  H +  K 
Sbjct: 119 MLDGMFSWVLYDQDKDKVVAARDPIGITTLYQGFSSDSPDTAYFASELKAL--HPVCDK- 175

Query: 172 NDAVIFDF---LYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEK 228
               I  F    YY+    T+ T    ++   P +   +D N        Y  LRE +E 
Sbjct: 176 ----IIAFPPGHYYD--SETKQT----VRYFKPSW---WDENKIPSNPVDYKLLRETLE- 221

Query: 229 RKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSI 269
                           +V+ RL+A+VP G  LSGGLDSS I
Sbjct: 222 ---------------ASVRKRLMAEVPYGVLLSGGLDSSLI 247


>pir||T16625 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) M02D8.4
           [similarity] - Caenorhabditis elegans
           >gi|1086745|gb|AAA82381.1| (U41034) coded for by C.
           elegans cDNA yk61f9.5; coded for by C. elegans cDNA
           yk80c1.5; coded for by C. elegans cDNA yk4g5.5; coded
           for by C. elegans cDNA yk127h1.5; coded for by C.
           elegans cDNA yk37d7.5; coded for by C. elegans cDNA
           yk55e11.5; coded fo>
           Length = 567
           
 Score =  101 bits (250), Expect = 2e-20
 Identities = 106/376 (28%), Positives = 173/376 (45%), Gaps = 51/376 (13%)

Query: 11  DEELVKRMNDAIRHRGPDDEGVYVDDNVS--LGHVRLAIIDLSPKGHQPMKYEKDGKEVW 68
           D +  ++++    HRGPD  G Y D      L H RLAI+DL     QP++     ++V 
Sbjct: 19  DLDEARKLSRRQHHRGPDFSGCYQDTKTGDILVHERLAIMDLGVV--QPLQGTAPNRQV- 75

Query: 69  IVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEW--GFDCVERFNGMWAFVIYD 126
            ++NGEIYN  E+R + E K Y   ++ D+EVI+  Y ++  G  C    +G++AFV+  
Sbjct: 76  -IHNGEIYNHQELR-DTELKKYNLKTHCDSEVIIFLYEKYRDGHIC-NLLDGVFAFVLC- 131

Query: 127 KSKNILFLSRDRFGIKPLYYYYDGK-NIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLL 185
                   +RD  G+K +YY  D      FS+E+K +                       
Sbjct: 132 -CDGDFLAARDPLGVKQMYYGIDDDGRYFFSNEMKCL----------------------- 167

Query: 186 DHTEDTFFEG-IKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLR 244
              ED+  +  I+   PG+         ++   +++D R+ I       D +  +   + 
Sbjct: 168 ---EDSCGDNKIESFPPGHYYTPKTGFVRYFNPEWFDFRKAIHP----LDLKLLQKTMIA 220

Query: 245 AVKMRLVADVPVGSCLSGGLDSSSI-VCAMRHLLPDG-EIKVFSLVFPGKEIDESKYQRI 302
           +V  RL++D P+G  LSGGLDSS +   A R +   G  +  FS+       D     R 
Sbjct: 221 SVHKRLMSDAPIGVLLSGGLDSSLVSSIASREMKRRGMAVHSFSIGVDHNSPDVVA-ARK 279

Query: 303 VVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYG---QYRVMKLARENGMKVL 359
           V      + +   F++E+ + ++  LI    E +   SI      Y + +  R+ G+KV+
Sbjct: 280 VAKFIGTTHHEFYFSIEEGIKNLRKLI-WHLESYDVTSIRASTPMYFLSEEIRKLGIKVV 338

Query: 360 LDGQGSDEILAGYHYF 375
           L G+G+DEI  GY YF
Sbjct: 339 LSGEGADEIFGGYLYF 354


>pir||G75092 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) PAB0750
           [similarity] - Pyrococcus abyssi (strain Orsay)
           >gi|5458556|emb|CAB50044.1| (AJ248286) asparagine
           synthetase, putative [Pyrococcus abyssi]
           Length = 479
           
 Score =  100 bits (247), Expect = 4e-20
 Identities = 117/401 (29%), Positives = 179/401 (44%), Gaps = 77/401 (19%)

Query: 1   MCGI-NGFSWGDEELVKRMNDAIRHRGPDDEGVYVD------DNVSL------GHVRLAI 47
           MC I  GF+   E+ +K +N   +HRGPD  G + +      DN S       G + L  
Sbjct: 1   MCLIAGGFNVSREDAIKIINLG-KHRGPDSFGFWSERQMLKSDNFSDVSEIEGGEIALIQ 59

Query: 48  IDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLE 107
             L+  G +    +    E+ +V+NGEIYN   +RK L E+G  F ++ D+EVIL     
Sbjct: 60  CRLAMTGSKSYT-QPFYNEIILVHNGEIYNHESLRKYLIERGVAFETDVDSEVILRLLEY 118

Query: 108 WGFD-----------CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGKNIIFS 156
             FD            ++   G +A   +   K  LF  RD  G++PLYY   G    F+
Sbjct: 119 LVFDRKLKVEEAIKLSMKMLRGDYAVAFFFNGKFYLF--RDPLGVRPLYYSSRGH---FA 173

Query: 157 SEIK---GILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFDLNTRK 213
           SE K   G+ +   P  P + V       ++  H       GI R+  G+N VF + T  
Sbjct: 174 SEKKVLWGLGEDAEPVLPGEIV-------SISKH-------GITRVR-GFN-VFSIRT-- 215

Query: 214 FEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSSIVCAM 273
            +   Y++               G ++L + +VK+R  A   VG   SGG+DSS I    
Sbjct: 216 -QGKDYFN---------------GLQNLLIDSVKVR--ASKKVGVLFSGGVDSSLIA--- 254

Query: 274 RHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQE 333
             L+      V       ++  + ++ R V +E  +    + F+ ED+  +I  ++   E
Sbjct: 255 --LIASRYSDVILYTSGTEDSKDVEWARRVAEELGLKLRESLFSREDIENEIERIMFAIE 312

Query: 334 E--PFSTLSIYGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
           E  P +       Y   K ARE+G+KVLL GQG+DE+  GY
Sbjct: 313 EPNPMNLAIAIPLYFSTKRAREDGVKVLLSGQGADELFGGY 353


>pir||G71050 asparagine synthase (glutamine-hydrolyzing) (EC 6.3.5.4) PH1102
           [similarity] - Pyrococcus horikoshii
           >gi|3257518|dbj|BAA30201.1| (AP000005) 480aa long
           hypothetical asparagine synthetase [Pyrococcus
           horikoshii]
           Length = 480
           
 Score = 98.3 bits (241), Expect = 2e-19
 Identities = 119/406 (29%), Positives = 178/406 (43%), Gaps = 87/406 (21%)

Query: 1   MCGI-NGFSWGDEELVKRMNDAIRHRGPDDEGVYVDDNVS-----------------LGH 42
           MC I  GF    +E++ R+ +  +HRGPD  GV+ +  V                  L  
Sbjct: 1   MCLIAGGFKLYTDEII-RIIELGKHRGPDSFGVWSEREVIKSSDFSALEEVKGGDKVLIQ 59

Query: 43  VRLAIIDLSPKGHQPMKYEKDGKEVWIVYNGEIYNFMEIRKELEEKGYTFTSNTDTEVIL 102
            RLA+   S +  QP        E+ +V+NGEIYN  ++R  L E+G +F S+ D+EVIL
Sbjct: 60  CRLAMTG-SKEYTQPFY-----NEIALVHNGEIYNHEKLRAYLVERGISFESDVDSEVIL 113

Query: 103 AAYLEWGFD-----------CVERFNGMWAFVIYDKSKNILFLSRDRFGIKPLYYYYDGK 151
                  FD            +    G +A   +   K  LF  RD  GI+PLYY   G 
Sbjct: 114 RLLEYLLFDRNLSPWNAIKKLMWMLRGDYAVAFHYNGKFYLF--RDPLGIRPLYYSSQGH 171

Query: 152 NIIFSSEIK---GILQHNIPRKPNDAVIFDFLYYNLLDHTEDTFFEGIKRLMPGYNAVFD 208
              F SE K   G+ +   P  P +AV             E + F GI R+      ++D
Sbjct: 172 ---FGSEKKVLWGLGEEATPVMPGEAV-------------EISKF-GINRV-----RLYD 209

Query: 209 LNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVADVPVGSCLSGGLDSSS 268
           +   K    +Y+D               G ++L + +VK+R  +   +G   SGGLDS+ 
Sbjct: 210 VTAIKSFGSEYFD---------------GLRNLLIDSVKIR--SSKKIGILFSGGLDSTL 252

Query: 269 IVCAMRHLLPDGEIKVFSLVFPGKEIDESKYQRIVVDECRVSWYRTTFNVEDVLADIIDL 328
           I       +      V   V   ++  + ++ R V  E  +      F+ E+V  ++  +
Sbjct: 253 IA-----FIASRYSNVTLYVSGTEDSKDVEWARKVSQELGLKLKEYIFSKEEVEKELKRI 307

Query: 329 IRTQEEPF-STLSI-YGQYRVMKLARENGMKVLLDGQGSDEILAGY 372
           +   EEP    LSI    Y   KLARE+G+K+LL GQG+DE+  GY
Sbjct: 308 MFAIEEPNPMNLSIAIPLYFSTKLAREDGVKILLSGQGADELFGGY 353


>emb|CAA62733.1| (X91388) unknown [Pseudomonas syringae]
           Length = 433
           
 Score = 84.7 bits (206), Expect = 3e-15
 Identities = 95/383 (24%), Positives = 155/383 (39%), Gaps = 29/383 (7%)

Query: 13  ELVKRMNDAIRHRGPDDEGVYVDDNVSLGHVRLAIIDLSPKGHQPMKYEKDGKEVWIVYN 72
           +++  M  A+  RGPD + V++  +++LGH    +        QP   E  G  V I+Y 
Sbjct: 27  KVILDMIAAMAGRGPDGQAVWLGPDLALGHCS-TVASRYDFNEQPWIGEIRGATVAIMYT 85

Query: 73  GEIYNFMEIRKELEEKGYTFTSNTDTEVILAAYLEWGFDCVERFNGMWAFVIYDKSKNIL 132
           G+IYN  ++++EL   G      +  E+I A  L  G+          + +  D  K  L
Sbjct: 86  GQIYNMWQLQQELASFGPAPDRWSYAEIIRAR-LWRGYGTARSALRACSLLPLDGQKREL 144

Query: 133 FLSRDRFGIKPLYYYYDGKNIIFSSEIKGILQHNIPRKPNDAVIFDFLYYNLLDHTEDTF 192
            L+RDR G++PLYY      IIF+S    I  H       D      +    +    +T 
Sbjct: 145 LLARDRLGLQPLYYTLTDGGIIFASVPTAIQAHPQFTAKLDRSALAIVLQPRMAMPGETP 204

Query: 193 FEGIKRLMPGYNAVFDLNTRKFEAWKYYDLRERIEKRKEISDPQGFKDLFLRAVKMRLVA 252
            +G+  L P    V   + R     +Y+ L                + L    V  +   
Sbjct: 205 LKGLHELPPA--NVLSFSKRGHTQVRYWQLESAPHTDNFEQTSDHVRALLEGVVARQFED 262

Query: 253 DVPVGSCLSGGLDSSSIVC-AMRHLLPDG---EIKVFSLVFPGKEID----------ESK 298
            VP  + LSGG+DS+S+    M+ L   G    +  F L F   E            ++ 
Sbjct: 263 QVPSAAMLSGGIDSTSVAALVMKQLATRGAGPALDTFCLKFDTDEDAFTSSELRPDIDAP 322

Query: 299 YQRIVVDECRVSWYRTTFNVEDVLADIIDLIRTQEEPFSTLSIYGQ-----YRVMKLARE 353
           +  +  +         +  ++D+L ++I   RT       L  +GQ     Y + +  R+
Sbjct: 323 FAALAAEYMGTRHTTLSATMQDLL-NVIPATRTAR----GLPGWGQFDASMYLLFERMRD 377

Query: 354 NGMKVLLDGQGSDEILAGYHYFF 376
           N   V L G+ +DEI  GY Y+F
Sbjct: 378 N-FSVALTGEAADEIFGGYPYYF 399


  Database: ./suso.pep
    Posted date:  Jul 6, 2001  5:57 PM
  Number of letters in database: 840,471
  Number of sequences in database:  2977
  
  Database: /banques/blast2/nr.pep
    Posted date:  Dec 14, 2000 12:46 PM
  Number of letters in database: 188,266,275
  Number of sequences in database:  595,510
  
Lambda     K      H
   0.323    0.142    0.438 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 250008298
Number of Sequences: 2977
Number of extensions: 11224040
Number of successful extensions: 29887
Number of sequences better than 1.0e-10: 80
Number of HSP's better than  0.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 62
Number of HSP's that attempted gapping in prelim test: 29463
Number of HSP's gapped (non-prelim): 199
length of query: 611
length of database: 189,106,746
effective HSP length: 53
effective length of query: 558
effective length of database: 157,386,935
effective search space: 87821909730
effective search space used: 87821909730
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 168 (69.9 bits)