BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1638 (gcvT) DE:PROBABLE AMINOMETHYLTRANSFERASE (EC 2.1.2.10)
(GLYCINE CLEAVAGE SYSTEM T PROTEIN)
(398 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UZP8|GCST_PYRAB PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 823 0.0
sp|O58888|GCST_PYRHO PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 804 0.0
sp|P54378|GCST_BACSU PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 296 4e-79
sp|Q9K934|GCST_BACHD PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 273 2e-72
sp|Q9WY54|GCST_THEMA PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 258 9e-68
sp|O67441|GCST_AQUAE PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 229 3e-59
sp|O86567|GCST_STRCO PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 224 2e-57
sp|P54261|GCST_SYNY3 PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 221 9e-57
gb|AAG19870.1| (AE005071) aminomethyltransferase; GcvT1 [Halobac... 206 4e-52
emb|CAC11159.1| (AL445063) probable aminomethyltransferase [Ther... 205 8e-52
sp|O32955|GCST_MYCLE PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 201 9e-51
pir||G70426 aminomethyltransferase (glycine cleavage system T pr... 196 3e-49
pir||D70786 probable gcvT protein - Mycobacterium tuberculosis (... 194 2e-48
sp|Q10376|GCST_MYCTU PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 194 2e-48
sp|Q9PGW5|GCST_XYLFA PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 191 1e-47
sp|P27248|GCST_ECOLI AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SY... 190 3e-47
sp|O49849|GCST_FLAAN AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 189 4e-47
gb|AAK41201.1| Aminomethyltransferase [Sulfolobus solfataricus] 188 9e-47
sp|P49363|GCST_FLAPR AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 187 1e-46
pir||G82994 glycine-cleavage system protein T1 PA5215 [imported]... 186 3e-46
pir||S38370 aminomethyltransferase (EC 2.1.2.10) precursor - gar... 184 1e-45
sp|P49364|GCST_PEA AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURS... 184 1e-45
sp|O23936|GCST_FLATR AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 184 2e-45
pir||T38631 aminomethyltransferase precursor - fission yeast (S... 183 4e-45
sp|Q9YBA2|GCST_AERPE PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 183 4e-45
sp|P93256|GCST_MESCR AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 181 1e-44
sp|P54260|GCST_SOLTU AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 180 2e-44
pir||T25732 hypothetical protein F25B4.1 - Caenorhabditis elegan... 173 4e-42
gb|AAF52996.1| (AE003629) CG6415 gene product [Drosophila melano... 173 4e-42
gb|AAC17627.1| (AC002131) Very strong similarity to aminomethylt... 171 2e-41
pir||C72551 probable aminomethyltransferase APE1695 - Aeropyrum ... 168 8e-41
sp|P48728|GCST_HUMAN AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 167 2e-40
gi|4502083 aminomethyltransferase (glycine cleavage system prote... 166 4e-40
sp|Q9TSZ7|GCST_CANFA AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 163 3e-39
sp|P25285|GCST_BOVIN AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 162 5e-39
sp|Q9K0L8|GCST_NEIMB PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 159 4e-38
sp|Q9JVP2|GCST_NEIMA PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CL... 159 4e-38
gb|AAB05000.1| (L41522) glycine cleavage T protein [Saccharomyce... 159 7e-38
gi|6320222 glycine cleavage T protein (T subunit of glycine deca... 159 7e-38
pir||A83341 glycine cleavage system protein T2 PA2442 [imported]... 158 1e-37
pir||JC6309 glycine cleavage T-protein - yeast (Saccharomyces ce... 155 1e-36
sp|P28337|GCST_CHICK AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECU... 144 2e-33
dbj|BAA12709.1| (D84656) ORF N313 [Schizosaccharomyces pombe] 141 1e-32
gi|7019365 dimethylglycine dehydrogenase precursor [Homo sapiens... 129 6e-29
gi|11417071 dimethylglycine dehydrogenase precursor [Homo sapiens] 127 2e-28
sp|Q63342|M2GD_RAT DIMETHYLGLYCINE DEHYDROGENASE, MITOCHONDRIAL ... 127 3e-28
sp|Q46337|SOXA_CORS1 SARCOSINE OXIDASE ALPHA SUBUNIT (SARCOSINE ... 125 7e-28
gb|AAF68432.3|AF239167_1 (AF239167) glycine cleavage system T-pr... 119 5e-26
pir||B82969 sarcosine oxidase alpha subunit PA5418 [imported] - ... 118 1e-25
gb|AAC48785.1| (AF026954) pyruvate dehydrogenase phosphatase reg... 118 1e-25
gi|11431492 aminomethyltransferase (glycine cleavage system prot... 118 1e-25
emb|CAB63337.1| (AL032631) contains similarity to Pfam domain: P... 116 6e-25
gb|AAD03414.1| (AF067650) sarcosine dehydrogenase [Rattus norveg... 115 1e-24
gb|AAD53398.2|AF095735_1 (AF095735) sarcosine dehydrogenase [Hom... 114 2e-24
gb|AAD33412.1| (AF129265) unknown [Homo sapiens] 112 8e-24
gb|AAD32214.1| (AF140745) sarcosine dehydrogenase [Homo sapiens]... 111 1e-23
gb|AAF45943.1| (AE003430) CG3626 gene product [Drosophila melano... 108 1e-22
sp|O87386|SOXA_RHIME SARCOSINE OXIDASE ALPHA SUBUNIT (SARCOSINE ... 99 1e-19
gb|AAG37965.1| (AC087079) Hypothetical protein Y37E3.u [Caenorha... 89 1e-16
gb|AAC31611.1| (AF082220) aminomethyltransferase [Bartonella gra... 85 1e-15
gb|AAC04876.1| (AF047332) glycine cleavage system T-protein [Dro... 69 9e-11
>sp|Q9UZP8|GCST_PYRAB PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|7450600|pir||C75088 probable aminomethyltransferase
(EC 2.1.2.10) (glycine cleavage system t protein)
PAB1638 - Pyrococcus abyssi (strain Orsay)
>gi|5458520|emb|CAB50008.1| (AJ248286) PROBABLE
AMINOMETHYLTRANSFERASE (EC 2.1.2.10) (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) [Pyrococcus abyssi]
Length = 398
Score = 823 bits (2102), Expect = 0.0
Identities = 398/398 (100%), Positives = 398/398 (100%)
Query: 1 MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK
Sbjct: 1 MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL
Sbjct: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
Query: 121 YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARW
Sbjct: 121 YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI
Sbjct: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDAL 300
KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDAL
Sbjct: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDAL 300
Query: 301 LKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEE 360
LKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEE
Sbjct: 301 LKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEE 360
Query: 361 YAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGLFRET 398
YAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGLFRET
Sbjct: 361 YAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGLFRET 398
>sp|O58888|GCST_PYRHO PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|7450602|pir||D71056 probable aminomethyltransferase
- Pyrococcus horikoshii >gi|3257563|dbj|BAA30246.1|
(AP000005) 401aa long hypothetical
aminomethyltransferase [Pyrococcus horikoshii]
Length = 401
Score = 804 bits (2055), Expect = 0.0
Identities = 383/398 (96%), Positives = 393/398 (98%)
Query: 1 MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
M KRVH+FDWHKEHA+KIEEFAGWEMPIWYSSIKEEHLAVRNAVG+FDVSHMGEI FRGK
Sbjct: 4 MVKRVHIFDWHKEHARKIEEFAGWEMPIWYSSIKEEHLAVRNAVGIFDVSHMGEIVFRGK 63
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
DALKFLQYVTTNDISKPPAISG YTLVLNERGAIKDETL+FNMGNNEYLMICDSDAFEKL
Sbjct: 64 DALKFLQYVTTNDISKPPAISGTYTLVLNERGAIKDETLVFNMGNNEYLMICDSDAFEKL 123
Query: 121 YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
YAWFTYLK+TIEQFTKLDLEIELKTYDIAMFAVQGPKARDLA+DLFGIDINEMWWFQARW
Sbjct: 124 YAWFTYLKRTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLAKDLFGIDINEMWWFQARW 183
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI
Sbjct: 184 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 243
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDAL 300
KP GLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDAL
Sbjct: 244 KPCGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDAL 303
Query: 301 LKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEE 360
LKQKERG+GRKLVHFKM+DKGIPREGYKVYANGE+IGEVTSGTLSPLLNVGIGIAFVKEE
Sbjct: 304 LKQKERGVGRKLVHFKMIDKGIPREGYKVYANGEMIGEVTSGTLSPLLNVGIGIAFVKEE 363
Query: 361 YAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGLFRET 398
YAKPGIEIEVEIRG RKKA+TVTPPFYDPKKYGLFRET
Sbjct: 364 YAKPGIEIEVEIRGQRKKAVTVTPPFYDPKKYGLFRET 401
>sp|P54378|GCST_BACSU PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|7450598|pir||H69958 aminomethyltransferase homolog
yqhI - Bacillus subtilis >gi|1303890|dbj|BAA12546.1|
(D84432) YqhI [Bacillus subtilis]
>gi|2634891|emb|CAB14388.1| (Z99116) similar to
aminomethyltransferase [Bacillus subtilis]
Length = 362
Score = 296 bits (749), Expect = 4e-79
Identities = 159/390 (40%), Positives = 231/390 (58%), Gaps = 33/390 (8%)
Query: 1 MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
M KR LFD +KE+ K +F GWE+P+ +SSIK+EH AVR A GLFDVSHMGE+ G
Sbjct: 1 MLKRTPLFDLYKEYGGKTIDFGGWELPVQFSSIKKEHEAVRTAAGLFDVSHMGEVEVSGN 60
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
D+L FLQ + TND+S YT + G D+ LI+ G N YL++ ++ +K
Sbjct: 61 DSLSFLQRLMTNDVSALTPGRAQYTAMCYPDGGTVDDLLIYQKGENRYLLVINASNIDKD 120
Query: 121 YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQ-AR 179
AW +++ D++I+ ++ IA+ AVQGPKA + ++L D++ + F
Sbjct: 121 LAW-------MKEHAAGDVQIDNQSDQIALLAVQGPKAEAILKNLTDADVSALKPFAFID 173
Query: 180 WVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYG 239
++ G K L+SR+GYTGE+G+E+Y + A+H+W++I++ G YG
Sbjct: 174 EADISGRKALISRTGYTGEDGYEIYCRS--------------DDAMHIWKKIIDAGDAYG 219
Query: 240 IKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK--DFIGK 297
+ P GLGARDTLR EA LYG E ++TP++A + FA+ K DF GK
Sbjct: 220 LIPCGLGARDTLRFEANVPLYGQELTR---------DITPIEAGIGFAVKHKKESDFFGK 270
Query: 298 DALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFV 357
L +QKE G RKLV +M++KGIPR GY+V+ NG+ +G+VT+GT SP L +G+A +
Sbjct: 271 SVLSEQKENGAKRKLVGLEMIEKGIPRHGYEVFQNGKSVGKVTTGTQSPTLGKNVGLALI 330
Query: 358 KEEYAKPGIEIEVEIRGARKKAITVTPPFY 387
E ++ G ++VEIR KA V PFY
Sbjct: 331 DSETSEIGTVVDVEIRKKLVKAKVVKTPFY 360
>sp|Q9K934|GCST_BACHD PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|10175437|dbj|BAB06535.1| (AP001516)
aminomethyltransferase [Bacillus halodurans]
Length = 365
Score = 273 bits (691), Expect = 2e-72
Identities = 153/387 (39%), Positives = 229/387 (58%), Gaps = 32/387 (8%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
K+ LFD ++++ K+ +F GW +P+ +SSIKEEH AVR GLFDVSHMGE+ G A
Sbjct: 5 KKTPLFDLYEQYGGKVIDFGGWALPVQFSSIKEEHEAVRTKAGLFDVSHMGEVEVTGAQA 64
Query: 63 LKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYA 122
L +LQ + TND+SK YT + E G D+ LI+ ++YL++ ++ +K A
Sbjct: 65 LNYLQRLVTNDVSKIKDGQAQYTAMCYENGGTVDDLLIYRRSEDQYLLVINAANIDKDIA 124
Query: 123 WFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQ-ARWV 181
W +E+ + I + A A+QGP A ++ + L + ++ +F+ V
Sbjct: 125 W-------MEKHAIDGVSITNVSNQTAQLALQGPVAENVLQTLTEEPLADIKFFRFVDGV 177
Query: 182 ELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIK 241
+ G+ +LLSR+GYTGE+GFE+Y E A +W++++E GK++G+
Sbjct: 178 NIAGVNVLLSRTGYTGEDGFELYC--------------LAEDAPVLWKKLIEAGKEHGVV 223
Query: 242 PAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK-DFIGKDAL 300
P GLGARDTLR EA LYG E + +++P++A + FA+ DK DFIGK+ L
Sbjct: 224 PCGLGARDTLRFEAKLPLYGQELTK---------DISPIEAGIGFAVKVDKEDFIGKEIL 274
Query: 301 LKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEE 360
KQKE+G RKLV +MVDKGIPR GY+VY + + IG VT+GT SP L +G+A ++ E
Sbjct: 275 KKQKEQGAPRKLVGLEMVDKGIPRTGYEVYVDNQKIGFVTTGTQSPTLKKNVGLALLQAE 334
Query: 361 YAKPGIEIEVEIRGARKKAITVTPPFY 387
+++ G E+ V +R + A V PFY
Sbjct: 335 HSELGTEVIVHVRKRQLIAKVVATPFY 361
>sp|Q9WY54|GCST_THEMA PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|7450604|pir||E72403 aminomethyltransferase -
Thermotoga maritima (strain MSB8)
>gi|4980709|gb|AAD35303.1|AE001706_2 (AE001706)
aminomethyltransferase [Thermotoga maritima]
Length = 364
Score = 258 bits (652), Expect = 9e-68
Identities = 150/387 (38%), Positives = 225/387 (57%), Gaps = 34/387 (8%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
KR LF+ H E K+ +FAGWEMP++Y+SI EE +AVR +VG+FDVSHMGE +G +A
Sbjct: 2 KRTPLFEKHVELGAKMVDFAGWEMPLYYTSIFEEVMAVRKSVGMFDVSHMGEFLVKGPEA 61
Query: 63 LKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYA 122
+ F+ ++ TND S P IY+++ NE G I D+ +++ + +E LM+ ++ EK +
Sbjct: 62 VSFIDFLITNDFSSLPDGKAIYSVMCNENGGIIDDLVVYKVSPDEALMVVNAANIEKDFN 121
Query: 123 WFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVE 182
W K D+E+ + A+ A QGPKA++ ++L + E+ ++ R
Sbjct: 122 WIKSHSK------NFDVEVSNISDTTALIAFQGPKAQETLQELVEDGLEEIAYYSFRKSI 175
Query: 183 LDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKP 242
+ G++ L+SR+GYTGE+GFE+ +E N A VW+ ++ +K +P
Sbjct: 176 VAGVETLVSRTGYTGEDGFELMLEAKN--------------APKVWDALMNLLRKIDGRP 221
Query: 243 AGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVT-PLQANLEFAIYWDKDFIGKDALL 301
AGLGARD RLEA Y LYG D+DE T P + L + + +KDF+GK+ALL
Sbjct: 222 AGLGARDVCRLEATYLLYGQ----------DMDENTNPFEVGLSWVVKLNKDFVGKEALL 271
Query: 302 KQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEY 361
K KE+ + RKLV ++ K I R+GY+V NGE +GE+TSG SP L I +A V +
Sbjct: 272 KAKEK-VERKLVALELSGKRIARKGYEVLKNGERVGEITSGNFSPTLGKSIALALVSKS- 329
Query: 362 AKPGIEIEVEIRGAR-KKAITVTPPFY 387
K G ++ V G + +A+ V PFY
Sbjct: 330 VKIGDQLGVVFPGGKLVEALVVKKPFY 356
>sp|O67441|GCST_AQUAE PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
Length = 350
Score = 229 bits (579), Expect = 3e-59
Identities = 138/380 (36%), Positives = 212/380 (55%), Gaps = 50/380 (13%)
Query: 7 LFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFL 66
L+ HK K FAGW MP+ Y+SI EE AVR G+FD+SHMG + +D K L
Sbjct: 11 LYYVHKHLKAKFTNFAGWTMPLQYTSIIEEVRAVRXRAGVFDISHMGRLLI--EDPEKKL 68
Query: 67 QYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTY 126
QY TTN++ K Y L+ NE+G IKD+ ++ + E+ + ++ +K+ W +
Sbjct: 69 QYFTTNNLDKLSVGKVQYNLLPNEKGGIKDDVTVYMLSEIEFFLCVNAANRQKVINWLSP 128
Query: 127 LKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVELDGI 186
L++ + ++ A+QGPK+ ++ + ++++ +++ + +
Sbjct: 129 -----------HLKLRDLSGELVQIALQGPKSEEIISKFY--PVSDLKYYRFKVFD---- 171
Query: 187 KMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLG 246
K ++SR+GYTGE+GFE+Y+ P+E K E LE K KP GLG
Sbjct: 172 KTIISRTGYTGEDGFEIYVS------PEEGK-----------ELFLELVKL--AKPCGLG 212
Query: 247 ARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDALLKQKER 306
ARD LR+EAG LYGNE E E+TP++ NLE + + K+FIGK+A+LK+K +
Sbjct: 213 ARDVLRIEAGLPLYGNELSE---------EITPIEVNLEKFVDFSKEFIGKEAMLKKKVK 263
Query: 307 GLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYAKPGI 366
+KL ++ +KGIPR+GY+V+ IG ++SGT SP LN GI + FV E K G
Sbjct: 264 ---KKLFGLELTEKGIPRKGYRVFKGDREIGWISSGTYSPTLNKGIALCFVDIEERKEGN 320
Query: 367 EIEVEIRGARKKAITVTPPF 386
E+E+E+RG R + + PF
Sbjct: 321 EVEIEVRGRRVRGVLRKYPF 340
>sp|O86567|GCST_STRCO PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|7479136|pir||T34752 aminomethyltransferase -
Streptomyces coelicolor >gi|3402239|emb|CAA20175.1|
(AL031184) aminomethyltransferase [Streptomyces
coelicolor A3(2)]
Length = 372
Score = 224 bits (564), Expect = 2e-57
Identities = 137/395 (34%), Positives = 208/395 (51%), Gaps = 35/395 (8%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
+R L H+ + +FAGW+MP+ Y S +EEH+AVR GLFD+SHMGEI G A
Sbjct: 7 RRTALDATHRALGATMTDFAGWDMPLRYGSEREEHVAVRTRAGLFDLSHMGEITVTGPQA 66
Query: 63 LKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYA 122
+ L + +I YT++ E G I D+ +++ + EY+++ ++ +
Sbjct: 67 AELLNFALVGNIGTVKPGRARYTMICREDGGILDDLIVYRLEEAEYMVVANASNAQ---- 122
Query: 123 WFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVE 182
L E+ D E+ A+ AVQGP++ + L D++ + ++
Sbjct: 123 --VVLDALTERAAGFDAEVRDDRDAYALLAVQGPESPGILASLTDADLDGLKYYAGLPGT 180
Query: 183 LDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKP 242
+ G+ L++R+GYTGE+GFE+++ +PE A+ +W+ + G+ G+ P
Sbjct: 181 VAGVPALIARTGYTGEDGFELFV--------------KPEHAVGLWQALTGAGEAAGLIP 226
Query: 243 AGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK--DFIGKDAL 300
GL RDTLRLEAG LYGNE LST +TP A L + ++K DF+G+ AL
Sbjct: 227 CGLSCRDTLRLEAGMPLYGNE------LST---ALTPFDAGLGRVVKFEKEGDFVGRAAL 277
Query: 301 LKQKERGLGRK---LVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFV 357
+ ER R LV + +PR GY+V A GE+IGEVTSG SP L I +A+V
Sbjct: 278 TEAAERAASRPPRVLVGLVAEGRRVPRSGYRVVAGGEVIGEVTSGAPSPTLGRPIAMAYV 337
Query: 358 KEEYAKPGIE-IEVEIRGARKKAITVTPPFYDPKK 391
+A PG E + V+IRG+ + V PFY +K
Sbjct: 338 DPAHAAPGTEGVGVDIRGSHEPYEVVALPFYKRQK 372
>sp|P54261|GCST_SYNY3 PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|7450597|pir||S76080 hypothetical protein -
Synechocystis sp. (strain PCC 6803)
>gi|1001434|dbj|BAA10058.1| (D63999)
aminomethyltransferase [Synechocystis sp.]
Length = 372
Score = 221 bits (558), Expect = 9e-57
Identities = 140/393 (35%), Positives = 205/393 (51%), Gaps = 41/393 (10%)
Query: 2 AKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
A R L++ E K+ F GWEMP+ ++ +K+EH AVR VG+FD+SHMG+ G+
Sbjct: 7 ALRTPLYNLITEQTTKLTTFGGWEMPVQFAGLKQEHQAVREKVGMFDISHMGKFVLTGQK 66
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNN-----EYLMICDSDA 116
L LQ + +D+ + YT++LN +G I D+ ++++ G N +I ++
Sbjct: 67 VLAALQSLVPSDLDRLTPGKAQYTVLLNAQGGIIDDIIVYDQGKNPEGQERVTLIVNAAT 126
Query: 117 FEKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWF 176
K W L+ E+ DL E + A+QGP+A + + L ++ E+ F
Sbjct: 127 TVKDKQWL--LEHLPEEIDFQDLSREK-----VLIALQGPEALTILQPLVDQNLGELPAF 179
Query: 177 QARWVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGK 236
E K ++R+GYTGE+GFE+ + PE +W+ G
Sbjct: 180 GHLEAEFLREKAFIARTGYTGEDGFEIMV--------------SPEVGKQLWQTF---GS 222
Query: 237 KYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK--DF 294
K G+ P GLGARDTLRLEAG LYG + DE TPL+A L + ++ D DF
Sbjct: 223 K-GVTPCGLGARDTLRLEAGMGLYGQDMN---------DETTPLEAGLGWLVHLDSKGDF 272
Query: 295 IGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGI 354
IG+ L +QK G+ ++LV +M+ K I R Y + NGE++G VTSGTLSP L I +
Sbjct: 273 IGRAVLTEQKANGVEKRLVGLEMLAKQIARHDYPILHNGEIMGIVTSGTLSPTLQKAIAL 332
Query: 355 AFVKEEYAKPGIEIEVEIRGARKKAITVTPPFY 387
+V E AK G E+EVE+RG V FY
Sbjct: 333 GYVPTELAKVGQELEVEVRGKTYGIKVVKKLFY 365
>gb|AAG19870.1| (AE005071) aminomethyltransferase; GcvT1 [Halobacterium sp. NRC-1]
Length = 387
Score = 206 bits (519), Expect = 4e-52
Identities = 130/386 (33%), Positives = 198/386 (50%), Gaps = 43/386 (11%)
Query: 11 HKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVT 70
H++ EF GW MP+ + I+ EH AVR A G+FDVSHMGEI G DA + +Q +T
Sbjct: 36 HEDRGASFTEFGGWNMPVDFDGIQAEHAAVREAAGIFDVSHMGEIEVSGPDAERLMQRLT 95
Query: 71 TNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNN---EYLMI----CDSDAFEKLYAW 123
TND+S+ Y + ++ G + D+T+++ + +L + D+ AF++ W
Sbjct: 96 TNDVSRLDPGDAQYAAITDDDGIMIDDTVVYRTPADWPGAFLFVPNAGHDAAAFDR---W 152
Query: 124 FTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVEL 183
+ LD ++ T D M AVQGP A DL G ++++ F+A V +
Sbjct: 153 TDH-----RDAHDLDASVDNVTTDYGMVAVQGPDAPDLVAARAGDGVHDLGRFEAATVGV 207
Query: 184 DGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPA 243
G++ L++ +GYTGE G E+ + A VW+ I + +P
Sbjct: 208 AGVECLVANTGYTGEAGVEIVF--------------PADGAGAVWDAIAND-----CQPC 248
Query: 244 GLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDALLKQ 303
GLGARDTLR+E G+ L G + + + TP +A + FA+ + F+G+DAL
Sbjct: 249 GLGARDTLRMEHGFLLSGQD------FDPEENPRTPFEAGIGFAVAPESGFVGRDALADT 302
Query: 304 KERGLGRKLVHFKMVDKGIPREGYKVYA-NGELIGEVTSGTLSPLLNVGIGIAFVKEEYA 362
++ V + ++G+PR GY V G+ IG VTSGT+SP L IG+ +V +A
Sbjct: 303 DSP--EQQFVGLTLDERGVPRHGYAVTTPAGDEIGTVTSGTMSPTLGEPIGLGYVDSAHA 360
Query: 363 KPGIEIEVEIRGARKKAITVTPPFYD 388
G + V IRG K+A TPPF D
Sbjct: 361 ADGTTVAVRIRGTDKQATITTPPFLD 386
>emb|CAC11159.1| (AL445063) probable aminomethyltransferase [Thermoplasma
acidophilum]
Length = 336
Score = 205 bits (516), Expect = 8e-52
Identities = 121/366 (33%), Positives = 200/366 (54%), Gaps = 41/366 (11%)
Query: 26 MPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPAISGIYT 85
MP++Y SI EEH+AVR VG+FDVSHMG++ GKDA FL ++ +S +YT
Sbjct: 1 MPLYYRSIIEEHMAVRKHVGIFDVSHMGDVTVSGKDASAFLDHMFPTKVSNLKNGECVYT 60
Query: 86 LVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYLKKTIEQFTKLDLEIELKT 145
LN+ G + D+T+++ MG + Y + ++ EK+Y W + + ++IE +
Sbjct: 61 AFLNDSGLMIDDTIVYRMGEDSYFFVPNAGTTEKIYRWVS------DHSAGYSVKIENVS 114
Query: 146 YDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVE---LDGI----KMLLSRSGYTGE 198
I+ A+QGP++ ++ +L + +F+ ++V ++ I ++++S +GYTGE
Sbjct: 115 NRISSIALQGPESEEVLNEL---GFSYPGYFKFQYVSGKYMNAITGKDQIIISGTGYTGE 171
Query: 199 NGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDTLRLEAGYT 258
G E I + E A+ +W+++LE K PAGLGARDTLR+E G
Sbjct: 172 KGVEFIIPN--------------EHAVELWKKLLEAINKRNGLPAGLGARDTLRMEKGML 217
Query: 259 LYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDALLKQKERGLGRKLVHFKMV 318
L G++ E + P +A++ F + D+DF+GK L + R ++ ++
Sbjct: 218 LSGHDFNE---------DRDPYEASVSFIVNNDEDFVGKKNL--EIRRRSDHEIFRGFVL 266
Query: 319 DKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYAKPGIEIEVEIRGARKK 378
GIPR G + A G+ +G VTSGT+SP+LN GI + ++ + Y+K E+ +EIR K
Sbjct: 267 SDGIPRNGNPIKAGGKRVGTVTSGTISPVLNKGIALGYIDKAYSKENTEVMIEIRSVDHK 326
Query: 379 AITVTP 384
A+ P
Sbjct: 327 AVVTKP 332
>sp|O32955|GCST_MYCLE PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|11280273|pir||T44888 probable aminomethyltransferase
(EC 2.1.2.10) [imported] - Mycobacterium leprae
>gi|2342607|emb|CAB11378.1| (Z98741)
aminomethyltransferase [Mycobacterium leprae]
Length = 367
Score = 201 bits (507), Expect = 9e-51
Identities = 139/387 (35%), Positives = 202/387 (51%), Gaps = 36/387 (9%)
Query: 7 LFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFL 66
L D H+E EF GW MP+ Y+ EH A RNAVGLFDVSH+G+ RG A +F+
Sbjct: 12 LEDRHRELGANFAEFGGWLMPVSYAGTVSEHSATRNAVGLFDVSHLGKALVRGPGAAQFV 71
Query: 67 QYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTY 126
V TND+ + YTL +E G + D+ + + + ++E ++ ++ A
Sbjct: 72 NSVLTNDLGRIRPGKAQYTLCCSESGGVIDDLIAYYVDDDEIFLVSNA-------ANTAA 124
Query: 127 LKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDL-FGIDINEMWWFQARWVELDG 185
+ ++ L I + A+ AVQGP++ D+ +L I+ M + A + G
Sbjct: 125 VVDALQAVVPAGLTIINQHRSHAVLAVQGPRSTDVLGELGLPTGIDYMGYVDASYA---G 181
Query: 186 IKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGL 245
+ + + R+GYTGE G+E+ P+ E A V++ ++ +PAGL
Sbjct: 182 VPVRVCRTGYTGEQGYEL----LPPW----------ESADVVFDALVAAVVDARGEPAGL 227
Query: 246 GARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKD-FIGKDALLKQK 304
GARDTLR E GY LYG+E LS DI +PLQA +AI W KD F+G+DALL +K
Sbjct: 228 GARDTLRTEMGYPLYGHE------LSLDI---SPLQARCGWAIGWKKDAFLGRDALLAEK 278
Query: 305 ERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYA-K 363
G R L +M +G+ R G V A IG TSGT SP L VG+ +A + E A +
Sbjct: 279 AAGPRRLLRGLRMAGRGVLRPGLTVCAGDIPIGVTTSGTFSPTLQVGVALALIDSEAAVQ 338
Query: 364 PGIEIEVEIRGARKKAITVTPPFYDPK 390
G +I V++RG + V PPF + K
Sbjct: 339 DGQQIIVDVRGRAVECEVVRPPFIEVK 365
>pir||G70426 aminomethyltransferase (glycine cleavage system T protein) -
Aquifex aeolicus >gi|2983847|gb|AAC07401.1| (AE000741)
aminomethyltransferase (glycine cleavage system T
protein) [Aquifex aeolicus]
Length = 301
Score = 196 bits (494), Expect = 3e-49
Identities = 119/341 (34%), Positives = 190/341 (54%), Gaps = 50/341 (14%)
Query: 46 LFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGN 105
+FD+SHMG + +D K LQY TTN++ K Y L+ NE+G IKD+ ++ +
Sbjct: 1 MFDISHMGRLLI--EDPEKKLQYFTTNNLDKLSVGKVQYNLLPNEKGGIKDDVTVYMLSE 58
Query: 106 NEYLMICDSDAFEKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDL 165
E+ + ++ +K+ W + L++ + ++ A+QGPK+ ++
Sbjct: 59 IEFFLCVNAANRQKVINWLSP-----------HLKLRDLSGELVQIALQGPKSEEIISKF 107
Query: 166 FGIDINEMWWFQARWVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKAL 225
+ ++++ +++ + + K ++SR+GYTGE+GFE+Y+ P+E K
Sbjct: 108 Y--PVSDLKYYRFKVFD----KTIISRTGYTGEDGFEIYVS------PEEGK-------- 147
Query: 226 HVWERILEEGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLE 285
E LE K KP GLGARD LR+EAG LYGNE E E+TP++ NLE
Sbjct: 148 ---ELFLELVKL--AKPCGLGARDVLRIEAGLPLYGNELSE---------EITPIEVNLE 193
Query: 286 FAIYWDKDFIGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLS 345
+ + K+FIGK+A+LK+K + +KL ++ +KGIPR+GY+V+ IG ++SGT S
Sbjct: 194 KFVDFSKEFIGKEAMLKKKVK---KKLFGLELTEKGIPRKGYRVFKGDREIGWISSGTYS 250
Query: 346 PLLNVGIGIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPF 386
P LN GI + FV E K G E+E+E+RG R + + PF
Sbjct: 251 PTLNKGIALCFVDIEERKEGNEVEIEVRGRRVRGVLRKYPF 291
>pir||D70786 probable gcvT protein - Mycobacterium tuberculosis (strain H37RV)
>gi|1237064|emb|CAA94254.1| (Z70283) gcvT [Mycobacterium
tuberculosis]
Length = 379
Score = 194 bits (488), Expect = 2e-48
Identities = 135/382 (35%), Positives = 194/382 (50%), Gaps = 34/382 (8%)
Query: 7 LFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFL 66
L D H+E EF GW MP+ Y+ EH A R AVGLFDVSH+G+ RG A +F+
Sbjct: 24 LEDRHRELGASFAEFGGWLMPVSYAGTVSEHNATRTAVGLFDVSHLGKALVRGPGAAQFV 83
Query: 67 QYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTY 126
TND+ + YTL E G + D+ + + + ++E ++ ++ A
Sbjct: 84 NSALTNDLGRIGPGKAQYTLCCTESGGVIDDLIAYYVSDDEIFLVPNA-------ANTAA 136
Query: 127 LKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVELDGI 186
+ ++ L I A+ AVQGP + D+ L G+ EM + G+
Sbjct: 137 VVGALQAAAPGGLSITNLHRSYAVLAVQGPCSTDVLTAL-GLP-TEMDYMGYADASYSGV 194
Query: 187 KMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLG 246
+ + R+GYTGE+G+E+ P+ E A V++ +L G +PAGLG
Sbjct: 195 PVRVCRTGYTGEHGYEL----LPPW----------ESAGVVFDALLAAVSAAGGEPAGLG 240
Query: 247 ARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKD-FIGKDALLKQKE 305
ARDTLR E GY L+G+E LS DI +PLQA +A+ W KD F G+ ALL +K
Sbjct: 241 ARDTLRTEMGYPLHGHE------LSLDI---SPLQARCGWAVGWRKDAFFGRAALLAEKA 291
Query: 306 RGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYA-KP 364
G R L +MV +G+ R G V E +G TSGT SP L VGIG+A + + +
Sbjct: 292 AGPRRLLRGLRMVGRGVLRPGLAVLVGDETVGVTTSGTFSPTLQVGIGLALIDSDAGIED 351
Query: 365 GIEIEVEIRGARKKAITVTPPF 386
G +I V++RG + V PPF
Sbjct: 352 GQQINVDVRGRAVECQVVCPPF 373
>sp|Q10376|GCST_MYCTU PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
Length = 367
Score = 194 bits (488), Expect = 2e-48
Identities = 135/382 (35%), Positives = 194/382 (50%), Gaps = 34/382 (8%)
Query: 7 LFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFL 66
L D H+E EF GW MP+ Y+ EH A R AVGLFDVSH+G+ RG A +F+
Sbjct: 12 LEDRHRELGASFAEFGGWLMPVSYAGTVSEHNATRTAVGLFDVSHLGKALVRGPGAAQFV 71
Query: 67 QYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTY 126
TND+ + YTL E G + D+ + + + ++E ++ ++ A
Sbjct: 72 NSALTNDLGRIGPGKAQYTLCCTESGGVIDDLIAYYVSDDEIFLVPNA-------ANTAA 124
Query: 127 LKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVELDGI 186
+ ++ L I A+ AVQGP + D+ L G+ EM + G+
Sbjct: 125 VVGALQAAAPGGLSITNLHRSYAVLAVQGPCSTDVLTAL-GLP-TEMDYMGYADASYSGV 182
Query: 187 KMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLG 246
+ + R+GYTGE+G+E+ P+ E A V++ +L G +PAGLG
Sbjct: 183 PVRVCRTGYTGEHGYEL----LPPW----------ESAGVVFDALLAAVSAAGGEPAGLG 228
Query: 247 ARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKD-FIGKDALLKQKE 305
ARDTLR E GY L+G+E LS DI +PLQA +A+ W KD F G+ ALL +K
Sbjct: 229 ARDTLRTEMGYPLHGHE------LSLDI---SPLQARCGWAVGWRKDAFFGRAALLAEKA 279
Query: 306 RGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYA-KP 364
G R L +MV +G+ R G V E +G TSGT SP L VGIG+A + + +
Sbjct: 280 AGPRRLLRGLRMVGRGVLRPGLAVLVGDETVGVTTSGTFSPTLQVGIGLALIDSDAGIED 339
Query: 365 GIEIEVEIRGARKKAITVTPPF 386
G +I V++RG + V PPF
Sbjct: 340 GQQINVDVRGRAVECQVVCPPF 361
>sp|Q9PGW5|GCST_XYLFA PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|11280272|pir||G82837 glycine cleavage T protein
XF0183 [imported] - Xylella fastidiosa (strain 9a5c)
>gi|9104982|gb|AAF82996.1|AE003872_7 (AE003872) glycine
cleavage T protein [Xylella fastidiosa]
Length = 368
Score = 191 bits (481), Expect = 1e-47
Identities = 136/395 (34%), Positives = 202/395 (50%), Gaps = 48/395 (12%)
Query: 1 MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
M K+ L D H+ K+ +F+GWEMPI Y S +EH VR G+FDVSHM I G
Sbjct: 1 MIKKTILNDTHRALGAKMVDFSGWEMPIHYGSQIDEHHHVRRNAGIFDVSHMTVIDLHGT 60
Query: 61 DALKFLQYVTTNDISKPPAI-SGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEK 119
L+ + N + K +Y+ +LN +G + D+ +++ + + + I ++ EK
Sbjct: 61 QVRPLLRRLLANSVDKLKVPGKALYSCMLNPQGGVIDDLIVYYLREDYFRFIVNAATREK 120
Query: 120 LYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKAR----DLARDLFGIDINEMWW 175
AW Q + ++ +E + D+AM AVQGP AR +L + + ++
Sbjct: 121 DLAWIN------TQASAFNVRVEERA-DLAMLAVQGPAARAQVTNLLAETHRDAVEKLGR 173
Query: 176 FQARWVELDGIKML-LSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEE 234
F A V K+L +SR+GYTGE+GFE+ + E+ + +W +L
Sbjct: 174 FAALEVASHSKKILFISRTGYTGEDGFEILLPQ--------------EETITLWNALL-- 217
Query: 235 GKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWD--K 292
K G+KP GLGARDTLRLEAG LYG + E +V+P +A L + + D +
Sbjct: 218 --KTGVKPIGLGARDTLRLEAGMNLYGQDMDE---------QVSPYEAALGWTVMLDEGR 266
Query: 293 DFIGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVY-ANGELIGEVTSGTLSPLLNVG 351
+FIG++ L +QK G+ R+++ M +KG+ R G KV A GE G + SGT SP LN
Sbjct: 267 NFIGRNVLEQQKTNGVSRQMIGLLMDEKGVLRHGQKVLTAQGE--GHILSGTFSPTLNKA 324
Query: 352 IGIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPF 386
IG F + KP E+ V IR V PF
Sbjct: 325 IG--FARVPAGKPS-EVRVNIRDREIPVRVVRFPF 356
>sp|P27248|GCST_ECOLI AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|1360976|pir||A56689 aminomethyltransferase (EC
2.1.2.10) [validated] - Escherichia coli
>gi|148040|gb|AAC36843.1| (M97263) T-protein
[Escherichia coli] >gi|403343|emb|CAA52144.1| (X73958)
aminomethyltransferase [Escherichia coli]
>gi|882435|gb|AAA69073.1| (U28377) ORF_f364 [Escherichia
coli] >gi|1789272|gb|AAC75943.1| (AE000374)
aminomethyltransferase (T protein;
tetrahydrofolate-dependent) of glycine cleavage system
[Escherichia coli K12]
Length = 364
Score = 190 bits (477), Expect = 3e-47
Identities = 131/395 (33%), Positives = 201/395 (50%), Gaps = 47/395 (11%)
Query: 1 MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
MA++ L++ H ++ +F GW MP+ Y S +EH AVR G+FDVSHM + RG
Sbjct: 1 MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDAGMFDVSHMTIVDLRGS 60
Query: 61 DALKFLQYVTTNDISK-PPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEK 119
+FL+Y+ ND++K + +Y+ +LN G + D+ +++ + + ++ +S EK
Sbjct: 61 RTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVYYFTEDFFRLVVNSATREK 120
Query: 120 LYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQAR 179
+W T + +EI ++ D++M AVQGP A+ A LF +
Sbjct: 121 DLSWIT------QHAEPFGIEITVRD-DLSMIAVQGPNAQAKAATLFNDAQRQAVEGMKP 173
Query: 180 WVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYG 239
+ + + ++ +GYTGE G+E+ + + EKA W ++E G
Sbjct: 174 FFGVQAGDLFIATTGYTGEAGYEIALPN--------------EKAADFWRALVEA----G 215
Query: 240 IKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYW---DKDFIG 296
+KP GLGARDTLRLEAG LYG E E ++PL AN+ + I W D+DFIG
Sbjct: 216 VKPCGLGARDTLRLEAGMNLYGQEMDE---------TISPLAANMGWTIAWEPADRDFIG 266
Query: 297 KDALLKQKERGLGRKLVHFKMVDKGIPREGYKV----YANGELIGEVTSGTLSPLLNVGI 352
++AL Q+E G KLV M +KG+ R V + G +TSGT SP L I
Sbjct: 267 REALEVQREHGT-EKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSI 325
Query: 353 GIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFY 387
+A V E + I V+IR R+ + VT P +
Sbjct: 326 ALARVPEGIGETAI---VQIRN-REMPVKVTKPVF 356
>sp|O49849|GCST_FLAAN AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|2894400|emb|CAA94902.1|
(Z71184) T-protein [Flaveria anomala]
Length = 407
Score = 189 bits (476), Expect = 4e-47
Identities = 130/400 (32%), Positives = 209/400 (51%), Gaps = 41/400 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H H K+ FAGW MPI Y SI + + R LFDVSHM + +GKD
Sbjct: 36 KKTVLYDFHVAHGGKMVPFAGWSMPIQYKDSIMDSTINCRENGSLFDVSHMCGLSLKGKD 95
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + D++ +G T+ NE+G D+++I + ++ ++ ++ +K
Sbjct: 96 CVAFLEKLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITKVTDDHIYLVVNAGCRDKDL 155
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDI-AMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ YD ++ A+QGP A + L D+++M++ R
Sbjct: 156 A---HIEEHMKAFKAKGGDVSWHIYDERSLLALQGPLAGSTLQHLTKEDLSKMYFGDFRI 212
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE--EGKKY 238
++++G K L+R+GYTGE+GFE+ + N A+ + + ILE EGK
Sbjct: 213 IDINGSKCFLTRTGYTGEDGFEISVPSEN--------------AVDLAKAILEKSEGK-- 256
Query: 239 GIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDF 294
++ GLGARD+LRLEAG LYGN+ ++ +TP++A L +AI + F
Sbjct: 257 -VRLTGLGARDSLRLEAGLCLYGNDMEQ---------HITPVEAGLTWAIGKRRRAEGGF 306
Query: 295 IGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGI 352
+G D +LKQ G + V + G P + N GE IGEVTSG SP L I
Sbjct: 307 LGADVILKQIADGPAIRRVG--LFSTGPPARSHSEIQNEKGENIGEVTSGGFSPCLKKNI 364
Query: 353 GIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
G+ +VK KPG ++++ IRG + PF K Y
Sbjct: 365 GMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMPFVPTKYY 404
>gb|AAK41201.1| Aminomethyltransferase [Sulfolobus solfataricus]
Length = 346
Score = 188 bits (473), Expect = 9e-47
Identities = 127/369 (34%), Positives = 198/369 (53%), Gaps = 68/369 (18%)
Query: 20 EFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPA 79
EFAGWEMP+ ++S EEH+ VRN+V F++SHMG + +G + + + +++K A
Sbjct: 19 EFAGWEMPMSFTSYAEEHMCVRNSVAFFNLSHMGRLRVKGNP--REFEMLVAKEVNK--A 74
Query: 80 ISGIY---TLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYLKKTIEQFTK 136
SGI T LN++ KD+ +++ + NE+L++ ++ EK+ W +
Sbjct: 75 NSGIMIGPTAFLNDKAGFKDDVMVYKVSENEWLIVTNAINREKIVNWIK---------SN 125
Query: 137 LDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEM----WWFQARWVELDGIKMLLSR 192
+L++E T+ M A+QG K ++ + NE+ + R++ D L+SR
Sbjct: 126 SNLDVEDLTFKYGMIAIQGRKLEEI------LGKNELKQLEFKLNTRFMGYD--VFLISR 177
Query: 193 SGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDTLR 252
SG+TGENG EV++ L V +++ + K GIKPAGL ARD+LR
Sbjct: 178 SGWTGENGLEVWV------------------TLDVGRQLMMDLVKIGIKPAGLIARDSLR 219
Query: 253 LEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYW----DKDFIGKDALLKQKERG 307
E G+ LYG DIDE +TP++A YW +KDFIGKD+L+++ G
Sbjct: 220 QEMGFVLYGE----------DIDETITPVEAR-----YWVFSLEKDFIGKDSLVERIRSG 264
Query: 308 LGRKLVHFKM--VDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYAKPG 365
+ + + FKM ++ IPR K+Y+ G +G VTS T SP LN IG+ +V +Y G
Sbjct: 265 IDKVRIGFKMRKGERTIPRHQSKIYSIGNEVGYVTSSTFSPYLNRVIGMGYVNPKYFYLG 324
Query: 366 IEIEVEIRG 374
+ EIRG
Sbjct: 325 YSLTGEIRG 333
>sp|P49363|GCST_FLAPR AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|542104|pir||S40217 T
protein - Flaveria pringlei >gi|1362139|pir||S56660
aminomethyltransferase (EC 2.1.2.10) precursor -
Flaveria pringlei >gi|438005|emb|CAA81077.1| (Z25858) T
protein [Flaveria pringlei]
Length = 407
Score = 187 bits (471), Expect = 1e-46
Identities = 130/400 (32%), Positives = 209/400 (51%), Gaps = 41/400 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H + K+ FAGW MPI Y SI E + R LFDVSHM + +GKD
Sbjct: 36 KKTVLYDFHVANGGKMVPFAGWSMPIQYKDSIMESTINCRENGSLFDVSHMCGLSLKGKD 95
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + D++ +G T+ NE+G D+++I + ++ ++ ++ +K
Sbjct: 96 CVPFLEKLVVADVAGLRPGTGSLTVFTNEKGGAIDDSVITKVTDDHIYLVVNAGCRDKDL 155
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDI-AMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ YD ++ A+QGP A + L D+++M++ R
Sbjct: 156 A---HIEEHMKAFKAKGGDVSWHIYDERSLLALQGPLAGSTLQHLTKEDLSKMYFGDFRI 212
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE--EGKKY 238
++++G K L+R+GYTGE+GFE+ + N A+ + + ILE EGK
Sbjct: 213 IDINGSKCFLTRTGYTGEDGFEISVPSEN--------------AVDLAKAILEKSEGK-- 256
Query: 239 GIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDF 294
++ GLGARD+LRLEAG LYGN+ ++ +TP++A L +AI + F
Sbjct: 257 -VRLTGLGARDSLRLEAGLCLYGNDMEQ---------HITPVEAGLTWAIGKRRRAEGGF 306
Query: 295 IGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGI 352
+G D +LKQ G + V + G P + N GE IGEVTSG SP L I
Sbjct: 307 LGADVILKQIADGPAIRRVG--LFSTGPPARSHSEIQNEKGENIGEVTSGGFSPCLKKNI 364
Query: 353 GIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
G+ +VK KPG ++++ IRG + PF K Y
Sbjct: 365 GMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMPFVPTKYY 404
>pir||G82994 glycine-cleavage system protein T1 PA5215 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9951522|gb|AAG08600.1|AE004934_7 (AE004934)
glycine-cleavage system protein T1 [Pseudomonas
aeruginosa]
Length = 360
Score = 186 bits (468), Expect = 3e-46
Identities = 137/394 (34%), Positives = 198/394 (49%), Gaps = 50/394 (12%)
Query: 1 MAKRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
M R L++ H K+ +F GW+MP+ Y S EEH VR G+FDVSHM + G+
Sbjct: 1 MGLRTPLYELHVALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMTVVDVAGE 60
Query: 61 DALKFLQYVTTNDISK-PPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEK 119
A +LQ++ ND+++ +Y+ +LNE G + D+ +++ + + Y ++ ++ +K
Sbjct: 61 QATAYLQHLLANDVARLGETGKALYSAMLNEEGGVVDDLIVY-LTEHGYRVVVNASTRDK 119
Query: 120 LYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKAR----DLARDLFGIDINEMWW 175
AW ++ F K+DL+ + D+AM A+QGP AR +L I E+
Sbjct: 120 DIAW---MQAQAAGF-KVDLQ---ERGDLAMLAIQGPNARVHSSELVSPARAALIRELKP 172
Query: 176 FQARWVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEG 235
FQ R E D ++R+GYTGE+G E+ + A L E
Sbjct: 173 FQGR-AEGD---WFIARTGYTGEDGLEIMLPAAEA------------------PGFLNEL 210
Query: 236 KKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWD---K 292
GI PAGLGARDTLRLEAG LYG + E V+PL AN+ + + W+ +
Sbjct: 211 VGAGISPAGLGARDTLRLEAGLNLYGQDMDE---------SVSPLAANMGWTVAWEPVAR 261
Query: 293 DFIGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGI 352
DF+G+ AL QK G KLV + ++G+ R V G GE+TSG+ SP LN I
Sbjct: 262 DFVGRRALEAQKAAGDQPKLVGLVLEERGVLRAHQVVRVAGIGEGEITSGSFSPTLNKSI 321
Query: 353 GIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPF 386
+A V A G EVEIRG V P F
Sbjct: 322 ALARVP---AATGDRAEVEIRGKWYPVRVVQPSF 352
>pir||S38370 aminomethyltransferase (EC 2.1.2.10) precursor - garden pea
>gi|407475|emb|CAA52800.1| (X74793) T-protein of the
glycine decarboxylase complex [Pisum sativum]
Length = 408
Score = 184 bits (463), Expect = 1e-45
Identities = 126/398 (31%), Positives = 208/398 (51%), Gaps = 37/398 (9%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H H K+ FAGW MPI Y SI + L R LFDVSHM + +GKD
Sbjct: 37 KKTVLYDFHVAHGGKMVPFAGWSMPIQYKDSIMDSTLNCRQNGSLFDVSHMCGLSLKGKD 96
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + D++ +G T+ NE+G D+++I + + ++ ++ +K
Sbjct: 97 VISFLEKLVIADVAALANGTGTLTVFTNEKGGAIDDSVITKVTEDHLYLVVNAGCRDKDL 156
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDI-AMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ +D ++ A+QGP A + + L D++++++ + R
Sbjct: 157 A---HIEEHMKAFKAKGGDVSWHIHDERSLLALQGPLAAPVLQHLTKEDLSKLYFGEFRV 213
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
++++G + L+R+GYTGE+GFE+ + H E + EK+ EGK I
Sbjct: 214 LDINGSQCFLTRTGYTGEDGFEISVPSE---HGVELAKALLEKS---------EGK---I 258
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDFIG 296
+ GLGARD+LRLEAG LYGN+ ++ +TP++A L +AI + F+G
Sbjct: 259 RLTGLGARDSLRLEAGLCLYGNDLEQ---------HITPIEAGLTWAIGKRRRAEGGFLG 309
Query: 297 KDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGIGI 354
D +LKQ G + V F + G P + + G IGEVTSG SP L I I
Sbjct: 310 ADVILKQLADGPSIRRVGF--ISSGPPPRSHSEIQDEGGNNIGEVTSGGFSPCLKKNIAI 367
Query: 355 AFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
+VK K G ++++ IRG + + + PF K Y
Sbjct: 368 GYVKSGLHKAGTKVKIIIRGKQNEGVVTKMPFVPTKYY 405
>sp|P49364|GCST_PEA AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|541970|pir||S40260
T-protein - garden pea >gi|1362061|pir||S56661
aminomethyltransferase (EC 2.1.2.10) precursor - garden
pea >gi|438217|emb|CAA81080.1| (Z25861) T-protein [Pisum
sativum] >gi|3021553|emb|CAA10976.1| (AJ222771) T
protein [Pisum sativum]
Length = 408
Score = 184 bits (463), Expect = 1e-45
Identities = 126/398 (31%), Positives = 209/398 (51%), Gaps = 37/398 (9%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H H K+ FAGW MPI Y SI + L R LFDVSHM + +GKD
Sbjct: 37 KKTVLYDFHVAHGGKMVPFAGWSMPIQYKDSIMDSTLNCRQNGSLFDVSHMCGLSLKGKD 96
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + D++ +G T+ NE+G D+++I + ++ ++ ++ +K
Sbjct: 97 VVSFLEKLVIADVAALAHGTGTLTVFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDL 156
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDI-AMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ +D ++ A+QGP A + + L D++++++ + R
Sbjct: 157 A---HIEEHMKAFKAKGGDVSWHIHDERSLLALQGPLAAPVLQHLTKEDLSKLYFGEFRV 213
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
++++G + L+R+GYTGE+GFE+ + H E + EK+ EGK I
Sbjct: 214 LDINGSQCFLTRTGYTGEDGFEISVPSE---HGVELAKALLEKS---------EGK---I 258
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDFIG 296
+ GLGARD+LRLEAG LYGN+ ++ +TP++A L +AI + F+G
Sbjct: 259 RLTGLGARDSLRLEAGLCLYGNDLEQ---------HITPIEAGLTWAIGKRRRAEGGFLG 309
Query: 297 KDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGIGI 354
D +LKQ G + V F + G P + + G IGEVTSG SP L I I
Sbjct: 310 ADVILKQLADGPSIRRVGF--ISSGPPPRSHSEIQDEGGNNIGEVTSGGFSPCLKKNIAI 367
Query: 355 AFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
+VK K G ++++ IRG + + + PF K Y
Sbjct: 368 GYVKSGLHKAGTKVKIIIRGKQNEGVVTKMPFVPTKYY 405
>sp|O23936|GCST_FLATR AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|2467117|emb|CAB16917.1|
(Z99769) T-Protein precursor [Flaveria trinervia]
Length = 407
Score = 184 bits (462), Expect = 2e-45
Identities = 128/400 (32%), Positives = 208/400 (52%), Gaps = 41/400 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H + K+ FAGW MPI Y SI E + R LFDVSHM + +GKD
Sbjct: 36 KKTVLYDFHVANGGKMVPFAGWSMPIQYKDSIMESTINCRENGSLFDVSHMCGLSLKGKD 95
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + D++ +G T+ NE+G D+++I + ++ ++ ++ +K
Sbjct: 96 CVAFLEKLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITKVTDDHIYLVVNAGCRDKDL 155
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDI-AMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ +D ++ A+QGP A + L D+++M++ R
Sbjct: 156 A---HIEQHMKAFKAKGGDVSWHIHDERSLLALQGPLAGSTLQHLTKDDLSKMYFGDFRI 212
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE--EGKKY 238
+++ G K L+R+GYTGE+GFE+ + N A+ + + ILE EGK
Sbjct: 213 IDISGSKCFLTRTGYTGEDGFEISVPSEN--------------AVDLAKAILEKSEGK-- 256
Query: 239 GIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDF 294
++ GLGARD+LRLEAG LYGN+ ++ +TP++A L +AI + F
Sbjct: 257 -VRLTGLGARDSLRLEAGLCLYGNDMEQ---------HITPVEAGLTWAIGKRRRAEGGF 306
Query: 295 IGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGI 352
+G + +LKQ G + V + G P + N GE IGEVTSG SP L I
Sbjct: 307 LGAEVILKQIADGPAIRRVG--LFSTGPPARSHSEIQNEQGENIGEVTSGGFSPCLKKNI 364
Query: 353 GIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
G+ +VK KPG ++++ IRG + PF K Y
Sbjct: 365 GMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMPFVPTKYY 404
>pir||T38631 aminomethyltransferase precursor - fission yeast
(Schizosaccharomyces pombe) >gi|2388965|emb|CAB11698.1|
(Z98979) putative aminomethyltransferase precursor
[Schizosaccharomyces pombe]
Length = 387
Score = 183 bits (459), Expect = 4e-45
Identities = 128/398 (32%), Positives = 201/398 (50%), Gaps = 42/398 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS--SIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
KR L+D H + I FAG+ MP+ Y +I H R GLFDVSHM + + RG+
Sbjct: 23 KRTPLYDLHLKEGATIVPFAGFSMPVQYKGQTISASHKWTREHSGLFDVSHMVQWFVRGE 82
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
+A +L+ +T + + + + NE G I D+T+I N Y ++ ++ EK
Sbjct: 83 NATAYLESITPSSLKELKPFHSTLSAFTNETGGIIDDTIISKQDENTYYIVTNAACSEKD 142
Query: 121 YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLF-GIDINEMWWFQAR 179
A LKK IE + ++LE + A+ A+QGP+ + + L +D + + + Q+
Sbjct: 143 EA---NLKKHIENWKGVELE---RVQGRALIAIQGPETASVVQKLIPNVDFSVLKFGQSA 196
Query: 180 WVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYG 239
+V+ G+K L SRSGYTGE+GFEV I + E ++ +L + +
Sbjct: 197 YVDFKGVKCLFSRSGYTGEDGFEVSIPE--------------EVSVDFASTLLADTR--- 239
Query: 240 IKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVT-PLQANLEFAI----YWDKDF 294
++P GLGARDTLRLEAG LYG +DID+ T P++ +L + I + F
Sbjct: 240 VRPIGLGARDTLRLEAGMCLYG----------SDIDDTTSPVEGSLSWIIGKRRRKEGGF 289
Query: 295 IGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGI 354
+G +LK+ + G R+ V F +V+K R G V +G +G+VTSG SP L I +
Sbjct: 290 VGSSRILKELKDGPSRRRVGF-IVEKVPARHGSAVEVDGVEVGQVTSGCPSPTLGKNIAM 348
Query: 355 AFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
++ + G +++R A V PF + Y
Sbjct: 349 GYISTGLHQVGTPAHIKVRNKLHPAQVVRMPFVETHYY 386
>sp|Q9YBA2|GCST_AERPE PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
Length = 375
Score = 183 bits (459), Expect = 4e-45
Identities = 130/390 (33%), Positives = 202/390 (51%), Gaps = 45/390 (11%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
+R L D H+ EFAGW +P+ Y +EH+AVR G+FD+SHMG + G+ A
Sbjct: 4 RRHILEDLHRSLGATFGEFAGWSVPMSYEGTLKEHMAVRREAGIFDISHMGRMIVSGEGA 63
Query: 63 LKFLQYVTTNDISKPPA--ISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
+ L+ + T +SK +SG TL LNE +KD+ + + +G E+L++ ++ + +
Sbjct: 64 TELLERIYTKRVSKTKVGFMSG-PTLALNEYARVKDDEMPYRLGEEEWLIVPNAAVADAM 122
Query: 121 YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
+F+ +I L++ I A+ A+QGP A + ++ G D+ ++ Q R
Sbjct: 123 LEYFS----SIASSMGLNVSIRDLRERYALLALQGPGAARVMEEMGGGDLLDLKPLQFR- 177
Query: 181 VELDGI----KMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGK 236
E GI ++SRSG+TGE+GFE+ E + +RI +
Sbjct: 178 -ENAGIAGVTAYIVSRSGWTGEDGFEIIAE------------------VEAAKRIFKAAV 218
Query: 237 KYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEF---AIYW-DK 292
+ G KPAG+ ARDTLR+EAG+ L G+E E L P +L + AI W K
Sbjct: 219 EAGAKPAGIAARDTLRIEAGFVLGGHEYGEDPLR-------WPCAVSLRYGLGAIDWGKK 271
Query: 293 DFIGKDALLKQKERG---LGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLN 349
F+G+ AL + G + LV K + IPR GY++Y + +G VTSGT SP++
Sbjct: 272 GFVGEAALRACRREGVRWIRVGLVMKKKYARMIPRSGYRLYVDDVDVGWVTSGTFSPVIG 331
Query: 350 VGIGIAFVKEEYAKPGIEIEVEIRGARKKA 379
G+ A++ YA G IEV++RG R +A
Sbjct: 332 RGVAQAYIDSRYAYIGDTIEVDVRGKRGEA 361
>sp|P93256|GCST_MESCR AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|7450599|pir||T12566
aminomethyltransferase (EC 2.1.2.10) precursor - common
ice plant >gi|1724108|gb|AAB38502.1| (U79769)
aminomethyltransferase precursor [Mesembryanthemum
crystallinum]
Length = 408
Score = 181 bits (455), Expect = 1e-44
Identities = 123/398 (30%), Positives = 210/398 (51%), Gaps = 37/398 (9%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H + K+ FAGW MPI Y SI + + R LFDV+HM + +GKD
Sbjct: 37 KKTALYDFHVANGGKMVPFAGWSMPIQYKDSIMDSTINCRENGSLFDVAHMCGLSLKGKD 96
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + DI+ +G +++ NE+G D+T+I + ++ ++ ++ EK
Sbjct: 97 CIPFLEKLVVGDIAGLAPGTGTLSVLTNEKGGAIDDTVITKVTDDHIYLVVNAGCREKDL 156
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYD-IAMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ +D ++ A+QGP A + + L D+++ ++ Q +
Sbjct: 157 A---HIEEHMKAFKAKGGDVSWHIHDERSLLALQGPLAAPVLQHLTKEDLSKFYFGQFTF 213
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
++++G L+R+GYTGE+GFE+ + N Y D +K EK+ EGK +
Sbjct: 214 LDINGFPCYLTRTGYTGEDGFEISV--PNEYAVDLAK-AMLEKS---------EGK---V 258
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDFIG 296
+ G GARD+LRLEAG LYGN+ ++ +TP++A L +A+ + F+G
Sbjct: 259 RLTGRGARDSLRLEAGLCLYGNDLEQ---------HITPIEAGLTWAVGKRRRAEGGFLG 309
Query: 297 KDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGIGI 354
+ +LKQ G ++ V F + G P G+ N GE IGE+TSG SP L I +
Sbjct: 310 AEVILKQIADGPPQRRVGF--ISSGPPARGHSEIQNEKGESIGEITSGGFSPCLKKNIAM 367
Query: 355 AFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
+VK K G ++ + +RG + + PF K Y
Sbjct: 368 GYVKSGNHKAGTKVNILVRGKPYEGVVTKMPFVPTKYY 405
>sp|P54260|GCST_SOLTU AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|2129976|pir||S59948
aminomethyltransferase (EC 2.1.2.10) precursor - potato
>gi|438254|emb|CAA81081.1| (Z25862) T-protein [Solanum
tuberosum]
Length = 406
Score = 180 bits (453), Expect = 2e-44
Identities = 125/400 (31%), Positives = 208/400 (51%), Gaps = 41/400 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H + K+ FAGW MPI Y SI + + R LFDVSHM + +GKD
Sbjct: 36 KKTVLYDFHVVNGGKMVPFAGWSMPIQYKDSIMDSTVNCRENGSLFDVSHMCGLSLKGKD 95
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + D++ +G T+ NE+G D++++ + N+ ++ ++ +K
Sbjct: 96 TIPFLEKLVIADVAGLAPGTGSLTVFTNEKGGAIDDSVVTKVTNDHIYLVVNAGCRDKDL 155
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDI-AMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ +D ++ A+QGP A + + L D+++M++ + R
Sbjct: 156 A---HIEEHMKSFKSKGGDVSWHIHDERSLLALQGPLAAPVLQYLTKDDLSKMYFGEFRV 212
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE--EGKKY 238
++++G L+R+GYTGE+GFE+ + E AL + + +LE EGK
Sbjct: 213 LDINGAPCFLTRTGYTGEDGFEISVPS--------------ENALDLAKALLEKSEGK-- 256
Query: 239 GIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDF 294
I+ GLGARD+LRLEAG LYGN+ ++ TP++A L +AI + F
Sbjct: 257 -IRLTGLGARDSLRLEAGLCLYGNDMEQ---------HTTPVEAGLTWAIGKRRRAEGGF 306
Query: 295 IGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVY--ANGELIGEVTSGTLSPLLNVGI 352
+G + +LKQ E G + V F G P + +NG+ IGE+TSG SP L I
Sbjct: 307 LGAEVILKQIEEGPKIRRVGF--FSSGPPPRSHSEIQDSNGQNIGEITSGGFSPCLKKNI 364
Query: 353 GIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
+ +VK K G +++ IRG + PF K Y
Sbjct: 365 AMGYVKTGNHKAGTNVKIVIRGKSYDGVVTKMPFVPTKYY 404
>pir||T25732 hypothetical protein F25B4.1 - Caenorhabditis elegans
>gi|1458286|gb|AAB37080.1| (U64842) partial CDS,
[Caenorhabditis elegans]
Length = 402
Score = 173 bits (433), Expect = 4e-42
Identities = 127/402 (31%), Positives = 207/402 (50%), Gaps = 39/402 (9%)
Query: 2 AKRVHLFDWHKEHAKKIEEFAGWEMPIWYS--SIKEEHLAVRNAVGLFDVSHMGEIYFRG 59
AK+ L + HK+H K+ EFAG++MP Y+ SIKE + R V LFDVSHM + Y G
Sbjct: 25 AKQTCLIETHKKHGGKLVEFAGYDMPTQYADFSIKESTIHTRKHVSLFDVSHMLQTYITG 84
Query: 60 KDALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEK 119
KD + F++ +TT D+ SG ++ NE+G IKD+ +I + ++ ++ EK
Sbjct: 85 KDRVAFIESLTTADVQGLQENSGTLSVFTNEKGGIKDDLIIMKTDKDFLFLVTNAGCIEK 144
Query: 120 LYAWFTYLKKTIEQFTKLDLEIELKTYD-IAMFAVQGPKARDLARDLFGIDINEMWWFQA 178
YL++ + +++++T D + AVQGP+ + ++ ID++++ + +
Sbjct: 145 ---DLPYLQENAAAWRSKGKDVKIETLDNRGLVAVQGPEMAKVLQEGTDIDLSKLTFMKT 201
Query: 179 RWVELDGIK-MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKK 237
++ GI ++R GYTGE+G E+ + +P KA + ER+L +
Sbjct: 202 TVGKVFGIDGCRVTRCGYTGEDGVEISV--------------DPTKAEQLVERLL-ASQA 246
Query: 238 YGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK----D 293
+K AGLGARD LRLEAG LYG++ +E TP++A L F + + D
Sbjct: 247 GSVKLAGLGARDALRLEAGLCLYGSDIEE---------NTTPIEAGLAFVVAKRRRETLD 297
Query: 294 FIGKDALLKQ-KERGLGRKLVHFKMVDKGIPREGYKVY--ANGELIGEVTSGTLSPLLNV 350
F G + ++KQ KE+ ++ V PR + + IG VTSG SP L
Sbjct: 298 FPGAEHIVKQLKEKSWPKRRVGLLAPAGRCPRSHLPLIDPLDKCSIGFVTSGCPSPTLGK 357
Query: 351 GIGIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
I IA+V + ++K G + V+ GA++ + V + P Y
Sbjct: 358 NIAIAYVDKSHSKIGTKFVVDF-GAKQAPVEVVKMPFVPTNY 398
>gb|AAF52996.1| (AE003629) CG6415 gene product [Drosophila melanogaster]
Length = 405
Score = 173 bits (433), Expect = 4e-42
Identities = 130/405 (32%), Positives = 206/405 (50%), Gaps = 45/405 (11%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS--SIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
+R L+D+H + KI F G+ +P+ YS SI HL R +FDVSHM + GK
Sbjct: 26 QRTALYDFHVQKGGKIVNFGGYALPVQYSDQSIIASHLHTRQVGSIFDVSHMLQSRIFGK 85
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
DA L+ V T DI P SG T+ NE G I D+ ++ + E ++ ++ E+
Sbjct: 86 DAAACLESVCTADILGTPEGSGSLTVFTNEAGGILDDLIVNKVSEKELYVVSNAAMKEQD 145
Query: 121 YAWFTYLKKTIEQFTK--LDLEIELKT-YDIAMFAVQGPK-ARDLARDLFG-IDINEMWW 175
+K ++ F D+ IE T D ++ AVQGP+ A++L++ L G ++++++
Sbjct: 146 MG---IMKTAVDNFKSQGKDVSIEFLTPADQSLVAVQGPQVAKELSKLLTGKASLDQLYF 202
Query: 176 FQARWVELDGI-KMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEE 234
+ L GI + ++R GYTGE+G E+ + + +A + E +LE
Sbjct: 203 MSSFVTTLAGIPNVRITRCGYTGEDGVEISVASS--------------QAQKLTESLLES 248
Query: 235 GKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDID-EVTPLQANLEFAI----Y 289
G +K AGLGARD+LRLEAG LYG +DID + TP++A L + +
Sbjct: 249 GV---LKLAGLGARDSLRLEAGLCLYG----------SDIDSKTTPVEAALAWLVTKRRR 295
Query: 290 WDKDFIGKDALLKQKERGLGRKLVHFKMVDKGIP--REGYKVYANGELIGEVTSGTLSPL 347
+DF G D +L Q + G+ R+ V +M+ P R G +++ G+ +G+VTSG SP
Sbjct: 296 TTRDFPGADVILGQLKEGVSRRRVGLQMLGTKPPPARSGVAIFSQGQQVGQVTSGCPSPS 355
Query: 348 LNVGIGIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
I + +V E PG ++E ++R +A PF Y
Sbjct: 356 AGRNIAMGYVAENLKAPGTKVEFKVRDKLYEAEVTKMPFVKANYY 400
>gb|AAC17627.1| (AC002131) Very strong similarity to aminomethyltransferase
precursor gb|U79769 from Mesembryanthemum crystallinum.
ESTs gb|T43167, gb|T21076, gb|H36999, gb|T22773,
gb|N38038, gb|T13742, gb|Z26545, gb|T20753 and gb|W43123
come from this gene. [Arabid>
Length = 408
Score = 171 bits (428), Expect = 2e-41
Identities = 119/400 (29%), Positives = 207/400 (51%), Gaps = 41/400 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L+D+H H K+ FAGW MPI Y SI + + R LFDV+HM + +GKD
Sbjct: 37 KKTALYDFHVAHGGKMVPFAGWSMPIQYKDSIMDSTVNCRENGSLFDVAHMCGLSLKGKD 96
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+ FL+ + D++ +G T+ NE+G D+++I + + ++ ++ +K
Sbjct: 97 CVPFLETLVVADVAGLAPGTGSLTVFTNEKGGAIDDSVITKVTDEHIYLVVNAGCRDKDL 156
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDI-AMFAVQGPKARDLARDLFGIDINEMWWFQARW 180
A ++++ ++ F ++ +D ++ A+QGP A + + L D++++++ +
Sbjct: 157 A---HIEEHMKAFKSKGGDVSWHIHDERSLLALQGPLAAPVLQHLTKEDLSKLYFGNFQI 213
Query: 181 VELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE--EGKKY 238
++++G L+R+GYTGE+GFE+ + D E A+ + + ILE EGK
Sbjct: 214 LDINGSTCFLTRTGYTGEDGFEISVPD--------------EHAVDLAKAILEKSEGK-- 257
Query: 239 GIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----YWDKDF 294
++ GLGARD+LRLEAG LYGN+ ++ ++P++A L +AI + F
Sbjct: 258 -VRLTGLGARDSLRLEAGLCLYGNDMEQ---------HISPVEAGLTWAIGKRRRAEGGF 307
Query: 295 IGKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVY--ANGELIGEVTSGTLSPLLNVGI 352
+G D +L+Q + G + V F G P + +G IGE+TSG SP L I
Sbjct: 308 LGADVILQQLKDGPTIRRVGF--FSSGPPARSHSEVHDESGNKIGEITSGGFSPNLKKNI 365
Query: 353 GIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
+ +VK K G ++++ +RG + PF K Y
Sbjct: 366 AMGYVKSGQHKTGTKVKILVRGKPYEGSITKMPFVATKYY 405
>pir||C72551 probable aminomethyltransferase APE1695 - Aeropyrum pernix (strain
K1) >gi|5105383|dbj|BAA80696.1| (AP000062) 349aa long
hypothetical aminomethyltransferase [Aeropyrum pernix]
Length = 349
Score = 168 bits (422), Expect = 8e-41
Identities = 121/367 (32%), Positives = 192/367 (51%), Gaps = 45/367 (12%)
Query: 26 MPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPA--ISGI 83
MP+ Y +EH+AVR G+FD+SHMG + G+ A + L+ + T +SK +SG
Sbjct: 1 MPMSYEGTLKEHMAVRREAGIFDISHMGRMIVSGEGATELLERIYTKRVSKTKVGFMSGP 60
Query: 84 YTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYLKKTIEQFTKLDLEIEL 143
TL LNE +KD+ + + +G E+L++ ++ + + +F+ + ++ L++ I
Sbjct: 61 -TLALNEYARVKDDEMPYRLGEEEWLIVPNAAVADAMLEYFSSIASSMG----LNVSIRD 115
Query: 144 KTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVELDGIK----MLLSRSGYTGEN 199
A+ A+QGP A + ++ G D+ ++ Q R E GI ++SRSG+TGE+
Sbjct: 116 LRERYALLALQGPGAARVMEEMGGGDLLDLKPLQFR--ENAGIAGVTAYIVSRSGWTGED 173
Query: 200 GFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDTLRLEAGYTL 259
GFE+ E + +RI + + G KPAG+ ARDTLR+EAG+ L
Sbjct: 174 GFEIIAE------------------VEAAKRIFKAAVEAGAKPAGIAARDTLRIEAGFVL 215
Query: 260 YGNETKELQLLSTDIDEVTPLQANLEF---AIYWDKD-FIGKDALLKQKERG---LGRKL 312
G+E E L P +L + AI W K F+G+ AL + G + L
Sbjct: 216 GGHEYGEDPLR-------WPCAVSLRYGLGAIDWGKKGFVGEAALRACRREGVRWIRVGL 268
Query: 313 VHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYAKPGIEIEVEI 372
V K + IPR GY++Y + +G VTSGT SP++ G+ A++ YA G IEV++
Sbjct: 269 VMKKKYARMIPRSGYRLYVDDVDVGWVTSGTFSPVIGRGVAQAYIDSRYAYIGDTIEVDV 328
Query: 373 RGARKKA 379
RG R +A
Sbjct: 329 RGKRGEA 335
>sp|P48728|GCST_HUMAN AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|484332|pir||JN0485 glycine
cleavage system T-protein precursor - human
>gi|391721|dbj|BAA02967.1| (D13811) glycine cleavage
system T-protein [Homo sapiens]
Length = 403
Score = 167 bits (418), Expect = 2e-40
Identities = 120/401 (29%), Positives = 196/401 (47%), Gaps = 41/401 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWY-SSIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
+R L+D+H H K+ FAGW +P+ Y S + HL R LFDVSHM + G D
Sbjct: 34 RRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSD 93
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+K ++ + DI++ G +L NE G I D+ ++ N ++ ++ +EK
Sbjct: 94 RVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDL 153
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWV 181
A ++ + + ++ L+ D A+ A+QGP A + + D+ ++ + + +
Sbjct: 154 A---LMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVM 210
Query: 182 ELDGIK-MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
E+ G+ ++R GYTGE+G E+ + A A+H+ IL K +
Sbjct: 211 EVFGVSGCRVTRCGYTGEDGVEISVPVAG--------------AVHLATAIL---KNPEV 253
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYWDK----DFI 295
K AGL ARD+LRLEAG LYGN DIDE TP++ +L + + + DF
Sbjct: 254 KLAGLAARDSLRLEAGLCLYGN----------DIDEHTTPVEGSLSWTLGKRRRAAMDFP 303
Query: 296 GKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGIG 353
G ++ Q + + R+ V ++ +G P + N G IG VTSG SP L +
Sbjct: 304 GAKVIVPQLKGRVQRRRV--GLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVA 361
Query: 354 IAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGL 394
+ +V EY++PG + VE+R ++ A+ PF Y L
Sbjct: 362 MGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTL 402
>gi|4502083 aminomethyltransferase (glycine cleavage system protein T) [Homo
sapiens] >gi|2134748|pir||I54192 aminomethyltransferase
(EC 2.1.2.10) precursor - human
>gi|994761|dbj|BAA03512.1| (D14686) glycine cleavage
system T-protein [Homo sapiens]
Length = 403
Score = 166 bits (416), Expect = 4e-40
Identities = 120/401 (29%), Positives = 195/401 (47%), Gaps = 41/401 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWY-SSIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
+R L+D+H H K+ FAGW +P+ Y S + HL R LFDVSHM + G D
Sbjct: 34 RRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSD 93
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
K ++ + DI++ G +L NE G I D+ ++ N ++ ++ +EK
Sbjct: 94 RCKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDL 153
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWV 181
A ++ + + ++ L+ D A+ A+QGP A + + D+ ++ + + +
Sbjct: 154 A---LMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVM 210
Query: 182 ELDGIK-MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
E+ G+ ++R GYTGE+G E+ + A A+H+ IL K +
Sbjct: 211 EVFGVSGCRVTRCGYTGEDGVEISVPVAG--------------AVHLATAIL---KNPEV 253
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYWDK----DFI 295
K AGL ARD+LRLEAG LYGN DIDE TP++ +L + + + DF
Sbjct: 254 KLAGLAARDSLRLEAGLCLYGN----------DIDEHTTPVEGSLSWTLGKRRRAAMDFP 303
Query: 296 GKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGIG 353
G ++ Q + + R+ V ++ +G P + N G IG VTSG SP L +
Sbjct: 304 GAKVIVPQLKGRVQRRRV--GLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVA 361
Query: 354 IAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGL 394
+ +V EY++PG + VE+R ++ A+ PF Y L
Sbjct: 362 MGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTL 402
>sp|Q9TSZ7|GCST_CANFA AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|6562361|emb|CAB62567.1|
(AJ012166) glycine cleavage system T-protein [Canis
familiaris]
Length = 403
Score = 163 bits (409), Expect = 3e-39
Identities = 115/401 (28%), Positives = 198/401 (48%), Gaps = 41/401 (10%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWY-SSIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
+R L+D+H H K+ FAGW +P+ Y S + HL R LFDVSHM + G D
Sbjct: 34 RRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHVDSHLHTRRHCSLFDVSHMLQTKILGCD 93
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
+K ++ + DI++ G +L NE G I+D+ ++ + ++ ++ ++K
Sbjct: 94 RVKLMESLVVGDIAELRPNQGTLSLFTNEAGGIEDDLIVTSTSEGYLYVVSNAGCWDKDL 153
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWV 181
A ++ + + + ++ L+ D A+ A+QGP A + + D+ ++ + + +
Sbjct: 154 A---LMQGKVRELQNMGSDVSLEVVDNALLALQGPTATQVLQAGVADDLRKLPFMTSAVM 210
Query: 182 ELDGIK-MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
E+ G+ ++R GYTGE+G E+ + A A+ + +LE + +
Sbjct: 211 EVFGVSGCRVTRCGYTGEDGVEISVPAA--------------AAVRLAAALLENPE---V 253
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYWDK----DFI 295
K AGL ARD+LRLEAG LYG+ DIDE TP++ +L + + + DF
Sbjct: 254 KLAGLAARDSLRLEAGLCLYGS----------DIDEHTTPVEGSLSWTLGKRRRAAMDFP 303
Query: 296 GKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGIG 353
G ++ Q + + R+ V + +G P + N G +IG VTSG SP L +
Sbjct: 304 GASVIIAQLKGKVQRRRVG--LTCEGAPVRAHSPILNMEGTVIGTVTSGCPSPCLKKNVA 361
Query: 354 IAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGL 394
+ +V EY++PG + VE+R ++ A+ PF Y L
Sbjct: 362 MGYVPSEYSRPGTPLLVEVRRKQQMAVVSKMPFVTTNYYTL 402
>sp|P25285|GCST_BOVIN AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|89579|pir||A23707
aminomethyltransferase (EC 2.1.2.10) precursor - bovine
>gi|163771|gb|AAA30786.1| (M59799) T-protein [Bos
taurus]
Length = 397
Score = 162 bits (407), Expect = 5e-39
Identities = 113/396 (28%), Positives = 185/396 (46%), Gaps = 37/396 (9%)
Query: 4 RVHLFDWHKEHAKKIEEFAGWEMPIWY-SSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
R L+D+H H K+ FAGW +P+ Y S HL R LFDVSHM + G D
Sbjct: 29 RTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHVNSHLHTRQHCSLFDVSHMLQTKIFGCDR 88
Query: 63 LKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYA 122
+K ++ + DI++ G +L NE G I D+ ++ + ++ ++ EK
Sbjct: 89 VKLMESLVVGDIAELKPNQGTLSLFTNEAGGILDDLIVTSASEGHLYVVSNAGCREKD-- 146
Query: 123 WFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVE 182
T ++ + + ++ L+ D A+ A+QGP A + + D+ ++ + + +E
Sbjct: 147 -LTLMQDKVRELQNKGSDVALEVMDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVME 205
Query: 183 LDGIK-MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIK 241
+ G+ ++R GYTGE+G E+ + A H + PE +K
Sbjct: 206 VFGVSGCRVTRCGYTGEDGVEISVPAAEAVHLAAALLKNPE-----------------VK 248
Query: 242 PAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYWDK----DFIG 296
AGL ARD+LRLEAG LYGN DIDE TP++ +L + + + DF G
Sbjct: 249 LAGLAARDSLRLEAGLCLYGN----------DIDEHTTPVEGSLSWTLGKRRRAAMDFPG 298
Query: 297 KDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAF 356
++ Q + R+ V + + + G +IG VTSG SP L + + +
Sbjct: 299 ASVIVPQLKSKAQRRRVGLMCDGAPVRAQSPILSPEGTVIGAVTSGCPSPCLKKNVAMGY 358
Query: 357 VKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
V EY++PG + VE+R ++ A+ PF Y
Sbjct: 359 VPYEYSRPGTPLLVEVRRKQQPAVVSKMPFVSTNYY 394
>sp|Q9K0L8|GCST_NEIMB PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|11280275|pir||A81183 glycine cleavage system T
protein NMB0574 [imported] - Neisseria meningitidis
(group B strain MD58) >gi|7225802|gb|AAF41002.1|
(AE002413) glycine cleavage system T protein [Neisseria
meningitidis MC58]
Length = 366
Score = 159 bits (399), Expect = 4e-38
Identities = 124/389 (31%), Positives = 192/389 (48%), Gaps = 57/389 (14%)
Query: 11 HKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVT 70
H++ K+ +FAGWE+PI Y S EH AVR G+FDVSHM G +A F + +
Sbjct: 13 HQDAGAKLVDFAGWELPIHYGSQIAEHEAVRTDAGMFDVSHMLVTDVAGANAKAFFRKLI 72
Query: 71 TNDISKPPAI-SGIYTLVLNERGAIKDETLIF--NMGNNEYLMICDSDAFEKLYAWFTYL 127
ND++K + +Y+ +LN+ G + D+ +++ N +Y ++ + EK A
Sbjct: 73 ANDVAKLAFVGKALYSALLNDNGGVIDDLIVYRTNEAETQYRIVSNGATREKDTA----- 127
Query: 128 KKTIEQFTKLDLEIEL---KTYDIAMFAVQGPKARD---LARDLFGIDINEMWWFQARWV 181
QF K+ E + YD+ M AVQGPKA + + + ++ + FQ +
Sbjct: 128 -----QFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKLLTVKPEWADVVHNLKPFQGADL 182
Query: 182 ELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIK 241
G ++R+GYTGE+G EV + P + + L++ G++
Sbjct: 183 ---GNDWFVARTGYTGEDGVEVIL---------------PGTEAVAFFKALQQA---GVQ 221
Query: 242 PAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYW---DKDFIGKD 298
P GLGARDTLR+EAG LYGN+ D+ +PL+A + + + +DF+GK
Sbjct: 222 PCGLGARDTLRMEAGMNLYGNDMD---------DDTSPLEAGMGWTVDLKDESRDFVGKA 272
Query: 299 ALLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN-GELIGEVTSGTLSPLLNVGIGIAFV 357
ALL KE+G+ K V + GI R +V + G+ GE TSG SP L I IA V
Sbjct: 273 ALLALKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQ--GETTSGVFSPSLKQSIAIARV 330
Query: 358 KEEYAKPGIEIEVEIRGARKKAITVTPPF 386
+++ G +V +RG + PF
Sbjct: 331 PKDF--DGDTAKVLMRGKEVDVRVLKLPF 357
>sp|Q9JVP2|GCST_NEIMA PROBABLE AMINOMETHYLTRANSFERASE (GLYCINE CLEAVAGE SYSTEM T PROTEIN)
>gi|11280274|pir||G81919 probable aminomethyltransferase
(EC 2.1.2.10) NMA0758 [imported] - Neisseria
meningitidis (group A strain Z2491)
>gi|7379479|emb|CAB84041.1| (AL162754) putative
aminomethyltransferase [Neisseria meningitidis Z2491]
Length = 368
Score = 159 bits (399), Expect = 4e-38
Identities = 124/388 (31%), Positives = 190/388 (48%), Gaps = 55/388 (14%)
Query: 11 HKEHAKKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVT 70
H++ K+ +FAGWE+PI Y S EH AVR G+FDVSHM G +A F + +
Sbjct: 15 HQDAGAKLVDFAGWELPIHYGSQIAEHEAVRTDAGMFDVSHMLVTDVAGANAKAFFRKLI 74
Query: 71 TNDISKPPAI-SGIYTLVLNERGAIKDETLIF--NMGNNEYLMICDSDAFEKLYAWFTYL 127
ND++K + +Y+ +LN+ G + D+ +++ N +Y ++ + EK A
Sbjct: 75 ANDVAKLAFVGKALYSALLNDNGGVIDDLIVYRTNEAETQYRIVSNGATREKDTA----- 129
Query: 128 KKTIEQFTKLDLEIELK---TYDIAMFAVQGPKARD---LARDLFGIDINEMWWFQARWV 181
QF K+ E + YD+ M AVQGPKA + + + ++ + FQ +
Sbjct: 130 -----QFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKLLTVKPEWADVVHNLKPFQGADL 184
Query: 182 ELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIK 241
G ++R+GYTGE+G EV + P + + L+ G++
Sbjct: 185 ---GNDWFVARTGYTGEDGVEVIL---------------PGTEAVAFFKALQAA---GVQ 223
Query: 242 PAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYW---DKDFIGKD 298
P GLGARDTLR+EAG LYGN+ D+ +PL+A + + + +DF+GK
Sbjct: 224 PCGLGARDTLRMEAGMNLYGNDMD---------DDTSPLEAGMGWTVDLKDESRDFVGKA 274
Query: 299 ALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVK 358
ALL KE+G+ K V + GI R +V + + GE TSG SP L I IA V
Sbjct: 275 ALLALKEKGVAVKQVGLLLGKGGILRAHMEVLTD-KGKGETTSGVFSPSLKQSIAIARVP 333
Query: 359 EEYAKPGIEIEVEIRGARKKAITVTPPF 386
+++ G +V IRG + PF
Sbjct: 334 KDF--DGDTAKVLIRGKEADVRVLKLPF 359
>gb|AAB05000.1| (L41522) glycine cleavage T protein [Saccharomyces cerevisiae]
Length = 400
Score = 159 bits (397), Expect = 7e-38
Identities = 129/397 (32%), Positives = 201/397 (50%), Gaps = 60/397 (15%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIK--EEHLAVRNAVGLFDVSHMGEIYFRGK 60
K+ L D H + +AG+ MP+ Y E H R GLFDVSHM + G
Sbjct: 17 KKTALHDLHVSLGGTMVPYAGYSMPVLYKGQTHIESHNWTRTNAGLFDVSHMLQSKLSGP 76
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGN-NEYLMICDSDAFEK 119
++KFLQ VT D + P SG +++LN +G + D+T+I + NE+ ++ ++ E+
Sbjct: 77 HSVKFLQRVTPTDFNALPVGSGTLSVLLNPQGGVVDDTIITKENDENEFYIVTNAGCAER 136
Query: 120 LYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLF-----GIDINEMW 174
+F ++ + LD + ++ ++ A+QGPKA+D+ L G D+ E++
Sbjct: 137 DTEFF---HDELQNGSTLDCQWKI-IEGRSLLALQGPKAKDVLEPLLSKTAPGKDLKELF 192
Query: 175 WFQARWVEL-DGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE 233
+ Q L DG + ++R GYTGE+GFE+ I + EKA+ E++L
Sbjct: 193 FGQRHEFALKDGSLVQIARGGYTGEDGFEISIAN--------------EKAVEFAEQLLA 238
Query: 234 EGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----- 288
+KP GL ARD+LRLEAG LYG+E E +TP++A L + I
Sbjct: 239 NPV---MKPIGLAARDSLRLEAGMCLYGHELDE---------SITPVEAALNWVISKSRR 286
Query: 289 ------YWDKDFIGKDALLKQ-KERGLGRKLVHFKMVDKG-IPREGYKVY---ANGELIG 337
YW F G ++ Q + + V FK + KG R G K++ A E +G
Sbjct: 287 DLVDQKYW---FNGYAKIMDQLNNKTYSKVRVGFKYLKKGPAARNGVKIFLPDAETE-VG 342
Query: 338 EVTSGTLSPLL-NVGIGIAFVKEEYAKPGIEIEVEIR 373
VTSG+ SP L N+ IG A+V++ Y K G ++ V++R
Sbjct: 343 LVTSGSASPTLNNINIGQAYVQKGYHKKGTKLLVQVR 379
>gi|6320222 glycine cleavage T protein (T subunit of glycine decarboxylase
complex; Gcv1p [Saccharomyces cerevisiae]
>gi|1707880|sp|P48015|GCST_YEAST AMINOMETHYLTRANSFERASE,
MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE SYSTEM T
PROTEIN) (GCVT) >gi|1077127|pir||S54642 glycine cleavage
T protein GCV1 - yeast (Saccharomyces cerevisiae)
>gi|840872|emb|CAA89844.1| (Z49770) Gcv1p [Saccharomyces
cerevisiae] >gi|1216226|emb|CAA65211.1| (X95966) glycine
cleavage T protein [Saccharomyces cerevisiae]
>gi|1431446|emb|CAA98840.1| (Z74315) ORF YDR019c
[Saccharomyces cerevisiae]
Length = 400
Score = 159 bits (397), Expect = 7e-38
Identities = 129/397 (32%), Positives = 201/397 (50%), Gaps = 60/397 (15%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIK--EEHLAVRNAVGLFDVSHMGEIYFRGK 60
K+ L D H + +AG+ MP+ Y E H R GLFDVSHM + G
Sbjct: 17 KKTALHDLHVSLGGTMVPYAGYSMPVLYKGQTHIESHNWTRTNAGLFDVSHMLQSKLSGP 76
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMG-NNEYLMICDSDAFEK 119
++KFLQ VT D + P SG +++LN +G + D+T+I +NE+ ++ ++ E+
Sbjct: 77 HSVKFLQRVTPTDFNALPVGSGTLSVLLNPQGGVVDDTIITKENDDNEFYIVTNAGCAER 136
Query: 120 LYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLF-----GIDINEMW 174
+F ++ + LD + ++ ++ A+QGPKA+D+ L G D+ E++
Sbjct: 137 DTEFF---HDELQNGSTLDCQWKI-IEGRSLLALQGPKAKDVLEPLLSKTAPGKDLKELF 192
Query: 175 WFQARWVEL-DGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE 233
+ Q L DG + ++R GYTGE+GFE+ I + EKA+ E++L
Sbjct: 193 FGQRHEFALKDGSLVQIARGGYTGEDGFEISIAN--------------EKAVEFAEQLLA 238
Query: 234 EGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----- 288
+KP GL ARD+LRLEAG LYG+E E +TP++A L + I
Sbjct: 239 NPV---MKPIGLAARDSLRLEAGMCLYGHELDE---------SITPVEAALNWVISKSRR 286
Query: 289 ------YWDKDFIGKDALLKQ-KERGLGRKLVHFKMVDKG-IPREGYKVY---ANGELIG 337
YW F G ++ Q + + V FK + KG R G K++ A E +G
Sbjct: 287 DLVDQKYW---FNGYAKIMDQLNNKTYSKVRVGFKYLKKGPAARNGVKIFLPDAETE-VG 342
Query: 338 EVTSGTLSPLL-NVGIGIAFVKEEYAKPGIEIEVEIR 373
VTSG+ SP L N+ IG A+V++ Y K G ++ V++R
Sbjct: 343 LVTSGSASPTLNNINIGQAYVQKGYHKKGTKLLVQVR 379
>pir||A83341 glycine cleavage system protein T2 PA2442 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9948489|gb|AAG05830.1|AE004671_6 (AE004671) glycine
cleavage system protein T2 [Pseudomonas aeruginosa]
Length = 373
Score = 158 bits (395), Expect = 1e-37
Identities = 125/394 (31%), Positives = 190/394 (47%), Gaps = 49/394 (12%)
Query: 11 HKEHAKKIEEFAGWEMPIWY-SSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYV 69
H E K+ FAG++MP+ Y + + +EHL R GLFDVSHMG+I G D L+ +
Sbjct: 15 HLELGAKMVPFAGYDMPVQYPAGVLKEHLHTREQAGLFDVSHMGQIRLVGADVALALESL 74
Query: 70 TTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYLKK 129
DI P Y L +E+G I D+ ++ N+G+ L++ + + L +L++
Sbjct: 75 VPVDILDLPVGQQRYALFTDEQGGILDDLMVANLGDCLLLVVNAACKHQDL----AHLRR 130
Query: 130 TIEQFTKLDLEIELKTYDIAMFAVQGPKA-RDLARDLFGIDINEMWWFQARWVELDGIKM 188
+E ++ E + A+ A+QGP A R L R + +M + Q VEL G
Sbjct: 131 HLEGRCSVEPLFE----ERALLALQGPAAVRVLER--LAPQVAQMTFMQFARVELLGQDC 184
Query: 189 LLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGAR 248
+SRSGYTGE+G+E+ + A+ EPE + P GLGAR
Sbjct: 185 YVSRSGYTGEDGYEISVPAAHAEALARRLLAEPE-----------------VAPIGLGAR 227
Query: 249 DTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYWDK--------DFIGKDA 299
D+LRLEAG LYG+ D+D TP++A+L +AI + F G +
Sbjct: 228 DSLRLEAGLCLYGH----------DMDSATTPVEASLGWAISKARRADGVRAGGFPGAER 277
Query: 300 LLKQKERGLGRKLVHFKMVDKGIPREGYKVY-ANGELIGEVTSGTLSPLLNVGIGIAFVK 358
+ Q+ +G+ K V F + REG ++ A G IG+V+SG P LN + + +V
Sbjct: 278 IFAQQAQGVASKRVGFLPQGRMPVREGAEIVDAQGRAIGKVSSGGFGPSLNAPLAMGYVP 337
Query: 359 EEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
E A G E+ +RG + PF + Y
Sbjct: 338 SELAGLGSEVTAMVRGKPVTLVVSKMPFVAQRYY 371
>pir||JC6309 glycine cleavage T-protein - yeast (Saccharomyces cerevisiae)
Length = 400
Score = 155 bits (387), Expect = 1e-36
Identities = 128/397 (32%), Positives = 200/397 (50%), Gaps = 60/397 (15%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYSSIK--EEHLAVRNAVGLFDVSHMGEIYFRGK 60
K+ L D H + +AG+ MP+ Y E H R GLFDVSHM + G
Sbjct: 17 KKTALHDLHVSLGGTMVPYAGYSMPVLYKGQTHIESHNWTRTNAGLFDVSHMLQSKLSGP 76
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMG-NNEYLMICDSDAFEK 119
++KFLQ VT D + P SG +++LN +G + D+T+I +NE+ ++ ++ E+
Sbjct: 77 HSVKFLQRVTPTDFNALPVGSGTLSVLLNPQGGVVDDTIITKENDDNEFYIVTNAGCRER 136
Query: 120 LYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLF-----GIDINEMW 174
+F ++ + LD + ++ ++ A+QGPKA+D+ L G D+ E++
Sbjct: 137 DTEFF---HDELQNGSTLDCQWKI-IEGRSLLALQGPKAKDVLEPLLSKTAPGKDLKELF 192
Query: 175 WFQARWVEL-DGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE 233
+ Q L DG + ++R GYTGE+GFE+ I + EKA+ E++L
Sbjct: 193 FGQRHEFALKDGSLVQIARGGYTGEDGFEISIAN--------------EKAVEFAEQLLA 238
Query: 234 EGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI----- 288
+KP GL ARD+LRLEAG L G+E E +TP++A L + I
Sbjct: 239 NPV---MKPIGLAARDSLRLEAGMCLTGHELDE---------SITPVEAALNWVISKSRR 286
Query: 289 ------YWDKDFIGKDALLKQ-KERGLGRKLVHFKMVDKG-IPREGYKVY---ANGELIG 337
YW F G ++ Q + + V FK + KG R G K++ A E +G
Sbjct: 287 DLVDQKYW---FNGYAKIMDQLNNKTYSKVRVGFKYLKKGPAARNGVKIFLPDAETE-VG 342
Query: 338 EVTSGTLSPLL-NVGIGIAFVKEEYAKPGIEIEVEIR 373
VTSG+ SP L N+ IG A+V++ Y K G ++ V++R
Sbjct: 343 LVTSGSASPTLNNINIGQAYVQKGYHKKGTKLLVQVR 379
>sp|P28337|GCST_CHICK AMINOMETHYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (GLYCINE CLEAVAGE
SYSTEM T PROTEIN) (GCVT) >gi|345695|pir||A44167
aminomethyltransferase (EC 2.1.2.10) precursor - chicken
>gi|222868|dbj|BAA01937.1| (D11162) T-protein [Gallus
gallus]
Length = 392
Score = 144 bits (359), Expect = 2e-33
Identities = 112/397 (28%), Positives = 179/397 (44%), Gaps = 37/397 (9%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKD 61
K+ L H+ ++ FAGW +P+ Y E HL R LFDVSHM + G+D
Sbjct: 23 KQTPLDALHRARGGRMVPFAGWSLPVQYGRGHLESHLHTRRHCSLFDVSHMLQTRVYGRD 82
Query: 62 ALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLY 121
++FL+ + DI++ G TL+ NERG I D+ ++ N + ++ ++ +K
Sbjct: 83 RVRFLESLVVGDIAELRPGQGTLTLLTNERGDIVDDLIVTNTAEDHLYVVSNAGCADKDR 142
Query: 122 AWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWV 181
A ++ + ++ L+ VQGP + + D+ ++ + +
Sbjct: 143 A---VMEGRAAELRAAGGDVHLEVSGQRAAGVQGPSMAQVLQAGLPDDLTKLTFMTSTAT 199
Query: 182 ELDGIK-MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGI 240
+ G+ ++R GYTGE+G E+ + E G PE VW
Sbjct: 200 TVFGVPGCRVTRCGYTGEDGVEISVPAGRAVELAERLLGCPE----VW------------ 243
Query: 241 KPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYWDK----DFI 295
PAGL ARD+LRLEAG LYGN DIDE TP++A L + + + DF
Sbjct: 244 -PAGLAARDSLRLEAGLCLYGN----------DIDESTTPVEAGLLWTLGKRRRTAMDFP 292
Query: 296 GKDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIA 355
G +++Q + RK V V + + G +G VTSG SP L I +
Sbjct: 293 GAAIIMEQVKEKPKRKRVGLTSVGPPLRPPAAILGPEGTPVGTVTSGCPSPSLGKNIAMG 352
Query: 356 FVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKY 392
+V+ +++PG + VE+R + A+ PF Y
Sbjct: 353 YVQAAHSRPGTTLTVEVRKKQHPALVTKMPFVPTHYY 389
>dbj|BAA12709.1| (D84656) ORF N313 [Schizosaccharomyces pombe]
Length = 313
Score = 141 bits (353), Expect = 1e-32
Identities = 98/313 (31%), Positives = 160/313 (50%), Gaps = 40/313 (12%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS--SIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
KR L+D H + I FAG+ MP+ Y +I H R GLFDVSHM + + RG+
Sbjct: 23 KRTPLYDLHLKEGATIVPFAGFSMPVQYKGQTISASHKWTREHSGLFDVSHMVQWFVRGE 82
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKL 120
+A +L+ +T + + + + NE G I D+T+I N Y ++ ++ EK
Sbjct: 83 NATAYLESITPSSLKELKPFHSTLSAFTNETGGIIDDTIISKQDGNTYYIVTNAACSEKD 142
Query: 121 YAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLF-GIDINEMWWFQAR 179
A LKK IE + ++LE + A+ A+QGP+ + + L +D + + + Q+
Sbjct: 143 EA---NLKKHIENWKGVELE---RVQGRALIAIQGPETASVVQKLIPNVDFSVLKFGQSA 196
Query: 180 WVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYG 239
+V+ G+K L SRSGYTGE+GFEV I + E ++ +L + +
Sbjct: 197 YVDFKGVKCLFSRSGYTGEDGFEVSIPE--------------EVSVDFASTLLADTR--- 239
Query: 240 IKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVT-PLQANLEFAI----YWDKDF 294
++P GLGARDTLRLE+G + + +D D+ T P++ +L + I + F
Sbjct: 240 VRPIGLGARDTLRLESG---------NVAFMESDTDDTTSPVEGSLSWIIGKRRRKEGGF 290
Query: 295 IGKDALLKQKERG 307
+G +LK+ + G
Sbjct: 291 VGSSRILKELKDG 303
>gi|7019365 dimethylglycine dehydrogenase precursor [Homo sapiens]
>gi|11133608|sp|Q9UI17|M2GD_HUMAN DIMETHYLGLYCINE
DEHYDROGENASE, MITOCHONDRIAL PRECURSOR (ME2GLYDH)
>gi|6650622|gb|AAF21941.1|AF111858_1 (AF111858)
dimethylglycine dehydrogenase precursor [Homo sapiens]
Length = 866
Score = 129 bits (321), Expect = 6e-29
Identities = 99/390 (25%), Positives = 182/390 (46%), Gaps = 57/390 (14%)
Query: 22 AGWEMP-------------------IWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
AGWE P W+ + E+ V VG+ D+S G+ +G+D+
Sbjct: 488 AGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVGVTDLSPFGKFNIKGQDS 547
Query: 63 LKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYA 122
++ L ++ N I K + + +L +G + E + + E+L+I S +
Sbjct: 548 IRLLDHLFANVIPKVGFTN--ISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLR 605
Query: 123 WFTYLKKTIEQFTKLDLEIELK--TYDIAMFAVQGPKARDLARDLFGIDINE--MWWFQA 178
W E+ K ++E+K T ++ + V GP+AR + + L D+++ + Q
Sbjct: 606 WIE------EEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTPEDLSDDVFKFLQT 659
Query: 179 RWVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKY 238
+ +++ I + R YTGE G+E+Y E ++ +++ I+ G++
Sbjct: 660 KSLKVSNIPVTAIRISYTGELGWELY--------------HRREDSVALYDAIMNAGQEE 705
Query: 239 GIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK--DFIG 296
GI G A + LRLE + +G E + PL+A LE+ + +K DFIG
Sbjct: 706 GIDNFGTYAMNALRLEKAFRAWGLEMN---------CDTNPLEAGLEYFVKLNKPADFIG 756
Query: 297 KDALLKQKERGLGRKLVHFKMVDKGIPREGYK-VYANGELIGEVTSGTLSPLLNVGIGIA 355
K AL + K +GL R+LV + + EG + ++ NG+++G TSG+ S + + A
Sbjct: 757 KQALKQIKAKGLKRRLVCLTLATDDVDPEGNESIWYNGKVVGNTTSGSYSYSIQKSLAFA 816
Query: 356 FVKEEYAKPGIEIEVEIRGARKKAITVTPP 385
+V + ++ G ++EVE+ G A+ + P
Sbjct: 817 YVPVQLSEVGQQVEVELLGKNYPAVIIQEP 846
>gi|11417071 dimethylglycine dehydrogenase precursor [Homo sapiens]
Length = 866
Score = 127 bits (317), Expect = 2e-28
Identities = 98/390 (25%), Positives = 181/390 (46%), Gaps = 57/390 (14%)
Query: 22 AGWEMP-------------------IWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
AGWE P W+ + E+ V V + D+S G+ +G+D+
Sbjct: 488 AGWEQPHWFYKPGQDTQYRPSFRRTNWFEPVGSEYKQVMQRVAVTDLSPFGKFNIKGQDS 547
Query: 63 LKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYA 122
++ L ++ N I K + + +L +G + E + + E+L+I S +
Sbjct: 548 IRLLDHLFANVIPKVGFTN--ISHMLTPKGRVYAELTVSHQSPGEFLLITGSGSELHDLR 605
Query: 123 WFTYLKKTIEQFTKLDLEIELK--TYDIAMFAVQGPKARDLARDLFGIDINE--MWWFQA 178
W E+ K ++E+K T ++ + V GP+AR + + L D+++ + Q
Sbjct: 606 WIE------EEAVKGGYDVEIKNITDELGVLGVAGPQARKVLQKLTSEDLSDDVFKFLQT 659
Query: 179 RWVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKY 238
+ +++ I + R YTGE G+E+Y E ++ +++ I+ G++
Sbjct: 660 KSLKVSNIPVTAIRISYTGELGWELY--------------HRREDSVALYDAIMNAGQEE 705
Query: 239 GIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK--DFIG 296
GI G A + LRLE + +G E + PL+A LE+ + +K DFIG
Sbjct: 706 GIDNFGTYAMNALRLEKAFRAWGLEMN---------CDTNPLEAGLEYFVKLNKPADFIG 756
Query: 297 KDALLKQKERGLGRKLVHFKMVDKGIPREGYK-VYANGELIGEVTSGTLSPLLNVGIGIA 355
K AL + K +GL R+LV + + EG + ++ NG+++G TSG+ S + + A
Sbjct: 757 KQALKQIKAKGLKRRLVCLTLATDDVDPEGNESIWYNGKVVGNTTSGSYSYSIQKSLAFA 816
Query: 356 FVKEEYAKPGIEIEVEIRGARKKAITVTPP 385
+V + ++ G ++EVE+ G A+ + P
Sbjct: 817 YVPVQLSEVGQQVEVELLGKNYPAVIIQEP 846
>sp|Q63342|M2GD_RAT DIMETHYLGLYCINE DEHYDROGENASE, MITOCHONDRIAL PRECURSOR (ME2GLYDH)
>gi|111583|pir||S16133 dimethylglycine dehydrogenase (EC
1.5.99.2) - rat >gi|56689|emb|CAA39468.1| (X55995)
dimethylglycine dehydrogenase [Rattus norvegicus]
Length = 857
Score = 127 bits (315), Expect = 3e-28
Identities = 107/391 (27%), Positives = 178/391 (45%), Gaps = 59/391 (15%)
Query: 22 AGWEMP-------------------IWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDA 62
AGWE P W+ + E+ V VG+ D+S G+ +G+D+
Sbjct: 481 AGWEQPHWFYKPGQDTQYRPSFRRTNWFRPVGSEYKQVMQRVGVIDLSPFGKFNIKGQDS 540
Query: 63 LKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYA 122
+ L ++ N I K + + +L RG + E + + E+L+I S +
Sbjct: 541 TQLLDHLCANVIPKVGFTN--ISHMLTPRGRVYAELTVSHQSPGEFLLITGSGSELHDLR 598
Query: 123 WFTYLKKTIEQFT---KLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINE--MWWFQ 177
W IE+ D+EI T ++ + V GP AR + + L D+++ + Q
Sbjct: 599 W-------IEEAAVRGGYDVEIRNITDELGVLGVAGPYARRVLQKLTSEDLSDDVFKFLQ 651
Query: 178 ARWVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKK 237
+ +++ I + R YTGE G+E+Y R E AL +ERI+ G++
Sbjct: 652 TKSLKISDIPVTAIRISYTGELGWELY------------HRREDSAAL--YERIMNAGQE 697
Query: 238 YGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK--DFI 295
GI G A + LRLE + +G+E + PL+A L++ I +K DF
Sbjct: 698 EGIDNFGTYALNALRLEKAFRAWGSEMN---------CDTNPLEAGLDYFIKLNKPADFT 748
Query: 296 GKDALLKQKERGLGRKLVHFKMVDKGIPREGYK-VYANGELIGEVTSGTLSPLLNVGIGI 354
GK AL + K +GL R+LV + + EG + V+ G++IG TSG+ S + +
Sbjct: 749 GKQALKQIKAKGLKRRLVCLTLATDDVDPEGNESVWYKGKVIGNTTSGSYSYSIQKSLAF 808
Query: 355 AFVKEEYAKPGIEIEVEIRGARKKAITVTPP 385
A+V E ++ G ++EVE+ G A + P
Sbjct: 809 AYVPVELSEVGQQVEVELLGKNYPATIIQEP 839
>sp|Q46337|SOXA_CORS1 SARCOSINE OXIDASE ALPHA SUBUNIT (SARCOSINE OXIDASE SUBUNIT)
>gi|2127435|pir||I40889 sarcosine oxidase (EC 1.5.3.1)
alpha chain [validated] - Corynebacterium sp
>gi|927591|gb|AAC43461.1| (U23955) sarcosine oxidase
alpha subunit [Corynebacterium sp.]
Length = 967
Score = 125 bits (312), Expect = 7e-28
Identities = 108/412 (26%), Positives = 185/412 (44%), Gaps = 49/412 (11%)
Query: 2 AKRVHLFDWHKEHAKKIEEFAGWEMPIWY---------SSIKEEHLAVRNAVGLFDVSHM 52
A+ + WH K E+ W+ WY +++ E AVR +VG+ D + +
Sbjct: 576 ARITPMHSWHLAQGAKFEDVGQWKRA-WYFPQDGEDMDAAVYRECAAVRESVGMLDATTL 634
Query: 53 GEIYFRGKDALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMIC 112
G+I RG DA +FL + TN +K Y ++ G + D+ + + + +LM
Sbjct: 635 GKIEIRGADAAEFLNRIYTNGYTKLKVGMARYGVMCKADGMVFDDGVTLRLAEDRFLMHT 694
Query: 113 DSDAFEKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARD-LARDLFGIDI- 170
+ + W +T ++ +LD+ T +A AV GP++RD +A+ + G+D+
Sbjct: 695 TTGGAAGVLDWLEEWLQT--EWPELDVTCTSVTEQLATVAVVGPRSRDVVAKLVTGLDVS 752
Query: 171 NEMWWFQA-RWVELD-GIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVW 228
N+ + F + + V LD GI+ +SR ++GE +E+ I +H L VW
Sbjct: 753 NDAFKFMSFQDVTLDSGIEARISRISFSGELAYEIAIPS---WH-----------GLRVW 798
Query: 229 ERILEEGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAI 288
E + G+++ I P G LR E G+ + G +T VTP A +E+ +
Sbjct: 799 EDVYAAGQEFNITPYGTETMHVLRAEKGFIIVGQDTD---------GTVTPQDAGMEWVV 849
Query: 289 YWDKDFIGKDALLKQKERGLGRK-LVHFKMVDKGIP-REGYKVYANG--------ELIGE 338
KDF+GK + ++ RK LV VD + EG + A+G + G
Sbjct: 850 SKLKDFVGKRSFSREDNLREDRKHLVSVLPVDTALRLAEGAALVADGAVETEGCTPMEGW 909
Query: 339 VTSGTLSPLLNVGIGIAFVKEEYAKPGIEIEVEIRGARKKAITVTPPFYDPK 390
VTS SP L G+A +K ++ G ++ + G + +DP+
Sbjct: 910 VTSSYNSPALGRTFGLALIKNGRSRIGEVLKTPVNGQLVDVLVSDLVLFDPE 961
>gb|AAF68432.3|AF239167_1 (AF239167) glycine cleavage system T-protein [Sus scrofa]
Length = 239
Score = 119 bits (296), Expect = 5e-26
Identities = 78/257 (30%), Positives = 129/257 (49%), Gaps = 22/257 (8%)
Query: 9 DWHKEHAKKIEEFAGWEMPIWY-SSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQ 67
D+H H K+ FAGW +P+ Y S + HL R LFDVSHM + G D +K ++
Sbjct: 1 DFHLAHGGKMVAFAGWSLPVQYRDSHVDSHLHTRQHCSLFDVSHMLQTKIFGSDRVKMME 60
Query: 68 YVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYL 127
+ DI++ G +L NE G I D+ ++ N ++ ++ EK T +
Sbjct: 61 SLVVGDIAELKPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCREK---DLTLM 117
Query: 128 KKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVELDGIK 187
+ + + +I L+ D A+ A+QGP A + + D+ ++ + + +E+ G+
Sbjct: 118 QDRVRELQNTGGDIGLEVMDNALLALQGPTAAQVLQAGVTDDLRKLPFMTSAVMEVFGVS 177
Query: 188 -MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLG 246
++R GYTGE+G E+ + A +A+ + +LE + +K AGL
Sbjct: 178 GCRVTRCGYTGEDGVEISVPAA--------------EAVRLATALLENPE---VKLAGLA 220
Query: 247 ARDTLRLEAGYTLYGNE 263
ARD+LRLEAG LYGN+
Sbjct: 221 ARDSLRLEAGLCLYGND 237
>pir||B82969 sarcosine oxidase alpha subunit PA5418 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9951745|gb|AAG08803.1|AE004954_5 (AE004954)
sarcosine oxidase alpha subunit [Pseudomonas aeruginosa]
Length = 1005
Score = 118 bits (293), Expect = 1e-25
Identities = 105/409 (25%), Positives = 180/409 (43%), Gaps = 54/409 (13%)
Query: 7 LFDWHKEHAKKIEEFAGWEMPIWY---------SSIKEEHLAVRNAVGLFDVSHMGEIYF 57
L WH ++ + E+ W+ P WY +++ E AVR AVGL D S +G+I
Sbjct: 619 LHAWHVKNGAEFEDVGQWKRP-WYFPRRGEDMHAAVARECRAVREAVGLLDASTLGKIDI 677
Query: 58 RGKDALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAF 117
+G DA +FL V TN +K Y L+ E G + D+ + + +N ++M +
Sbjct: 678 QGPDAREFLNRVYTNAWTKLDVGKARYGLMCKEDGMVFDDGVTACLADNHFVMTTTTGGA 737
Query: 118 EKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQ 177
++ W +T ++ +L + T A + GP +R L ++ ID+++ +
Sbjct: 738 ARVLEWLELYHQT--EWPELKVYFTSVTDHYATLTLSGPNSRKLLAEVTDIDLDKDAFPF 795
Query: 178 ARWVE--LDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEG 235
W E + G+ + R +TGE +EV + + + A+ V E + E G
Sbjct: 796 MTWKEGKVAGVPARVFRISFTGELSYEVNV--------------QADYAMGVLEALAEHG 841
Query: 236 KKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKDF- 294
KYG+ P G LR E G+ + G +T VTP N+ +A+ K F
Sbjct: 842 AKYGLTPYGTETMHVLRAEKGFIIVGQDTDA---------SVTPDDLNMGWAVGRSKPFS 892
Query: 295 -IG------KDALLKQKERGLGRKLVHFKMVDKGIPREGYKVYANGE-----LIGEVTSG 342
IG D L + +++ +G + + + V +P V+ + ++G VTS
Sbjct: 893 WIGWRGMNRADCLREDRKQLVGLRPSNPQEV---LPEGAQLVFDTQQAIPMKMVGHVTSS 949
Query: 343 TLSPLLNVGIGIAFVKEEYAKPGIEIEVEIRGAR-KKAITVTPPFYDPK 390
+S L G +A VK + G ++ + R +A + FYDPK
Sbjct: 950 YMSASLGHGFALAVVKGGLKRMGQKVYAPLADGRFIEAEICSSVFYDPK 998
>gb|AAC48785.1| (AF026954) pyruvate dehydrogenase phosphatase regulatory subunit
precursor; PDPr [Bos taurus]
Length = 878
Score = 118 bits (292), Expect = 1e-25
Identities = 100/354 (28%), Positives = 164/354 (46%), Gaps = 45/354 (12%)
Query: 18 IEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMG--EIYFRGKDALKFLQYVTTNDIS 75
+E+ + P W+ ++ E + AV + D+S EI G AL+ LQY+ +ND+
Sbjct: 494 LEQSKTFYKPDWFEIVESEVKCCKEAVCVIDMSSFTKFEITSTGDQALEILQYLFSNDLD 553
Query: 76 KPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYLKKTIEQFT 135
P ++T +LNERG +++ I + + MI +D +AW LKK + + +
Sbjct: 554 VPVGHI-VHTGMLNERGGYENDCSIARLSKRSFFMISPTDQQVHCWAW---LKKYMPEDS 609
Query: 136 KLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVEL-----DGIKMLL 190
L LE T+ + GP+ D+ +L + + E+ +GI+++
Sbjct: 610 NLILEDV--TWKYTALNLIGPRTVDVLSELSYAPMTPDHFPSLFCKEMSVGYANGIRVMS 667
Query: 191 SRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDT 250
+TGE GF +YI E ALHV+ ++ G+KYGI+ AG A +
Sbjct: 668 MT--HTGEPGFMLYIPI--------------EYALHVYNEVMSVGQKYGIRNAGYYALRS 711
Query: 251 LRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDK--DFIGKDALLKQKERGL 308
LR+E + +G + L TPL+ E + DK DFIG+DALL+Q++ G+
Sbjct: 712 LRIEKFFAFWGQDLNTL---------TTPLECGGESRVKLDKGVDFIGRDALLQQRQNGV 762
Query: 309 GRKLVHFKMVDKGI-----PREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFV 357
+L F + D P G +Y NG +G+ TS L + + FV
Sbjct: 763 YNRLTMFILDDHDTDLDLWPWWGEPIYRNGRYVGKTTSSAYGYTLERHVCLGFV 816
>gi|11431492 aminomethyltransferase (glycine cleavage system protein T) [Homo
sapiens]
Length = 306
Score = 118 bits (292), Expect = 1e-25
Identities = 92/337 (27%), Positives = 158/337 (46%), Gaps = 40/337 (11%)
Query: 66 LQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFT 125
++ + DI++ G +L NE G I D+ ++ N ++ ++ +EK A
Sbjct: 1 MESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLA--- 57
Query: 126 YLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVELDG 185
++ + + ++ L+ D A+ A+QGP A + + D+ ++ + + +E+ G
Sbjct: 58 LMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFG 117
Query: 186 IK-MLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAG 244
+ ++R GYTGE+G E+ + A H + PE +K AG
Sbjct: 118 VSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPE-----------------VKLAG 160
Query: 245 LGARDTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAIYWDK----DFIGKDA 299
L ARD+LRLEAG LYGN DIDE TP++ +L + + + DF G
Sbjct: 161 LAARDSLRLEAGLCLYGN----------DIDEHTTPVEGSLSWTLGKRRRAAMDFPGAKV 210
Query: 300 LLKQKERGLGRKLVHFKMVDKGIPREGYKVYAN--GELIGEVTSGTLSPLLNVGIGIAFV 357
++ Q + + R+ V ++ +G P + N G IG VTSG SP L + + +V
Sbjct: 211 IVPQLKGRVQRRRVG--LMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNVAMGYV 268
Query: 358 KEEYAKPGIEIEVEIRGARKKAITVTPPFYDPKKYGL 394
EY++PG + VE+R ++ A+ PF Y L
Sbjct: 269 PCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTL 305
>emb|CAB63337.1| (AL032631) contains similarity to Pfam domain: PF01571 (Glycine
cleavage T-protein (aminomethyl transferase)),
Score=250.7, E-value=6.4e-72, N=1~cDNA EST EMBL:Z14769
comes from this gene~cDNA EST EMBL:M89219 comes from
this gene~cDNA EST yk17f7.3 comes>
Length = 872
Score = 116 bits (287), Expect = 6e-25
Identities = 91/368 (24%), Positives = 170/368 (45%), Gaps = 50/368 (13%)
Query: 27 PIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPAISGIYTL 86
P W+ + E+ A R VGL D+S + G+DA+++LQ++ + ++ +P + +YT
Sbjct: 510 PEWFERVASEYEACRERVGLMDMSSFSKYDITGEDAVEYLQFLCSANVDEPIGTT-VYTG 568
Query: 87 VLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYLKKTIEQFTKLDLEIELKTY 146
+ +++G + + +G ++ M+ + E++ W K + K + ++ T
Sbjct: 569 MQHQKGGYVTDCTLSRLGEKKFFMVAPTIQQERVLVWM----KKWQAILKARVHVQDVTG 624
Query: 147 DIAMFAVQGPKARDLARDLFGIDI--NEMWWFQARWVEL---DGIKMLLSRSGYTGENGF 201
+ GP +R L D+ G+ + N+ F+ + + + GI+ + + GE G+
Sbjct: 625 AYTALDLIGPSSRYLMGDITGLSMSSNDFPTFRCQEINIGMATGIRAI--SVTHCGELGW 682
Query: 202 EVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDTLRLEAGYTLYG 261
+Y+ + E A +V+E+IL+ GK+Y ++ AG LR+E Y +G
Sbjct: 683 VIYVPN--------------EVAQNVYEKILDAGKEYSLQHAGYYTLRQLRIEKFYVYWG 728
Query: 262 NETKELQLLSTDIDEVTPLQANLEFAIYWDKDFIGKDALLKQKERGLGRKLV------HF 315
+ VTP++ F + + KDFIGK AL +Q ERG+ ++ V H
Sbjct: 729 QDINA---------TVTPVECGRLFRVDFKKDFIGKKALEEQVERGVSKRFVQLLVDGHD 779
Query: 316 KMVDKGIPREGYKVYANGELIGEVTSGTLSPLLNVGIGIAFVKE-------EYAKPGIEI 368
K D P+ G + +G +G TS L + I +V+ E+ G
Sbjct: 780 KETDPW-PQGGETILKDGRAVGLTTSAAYGFTLGCQVCIGYVENKEFGVSPEFVSSG-HF 837
Query: 369 EVEIRGAR 376
E++I G R
Sbjct: 838 EIDIAGKR 845
>gb|AAD03414.1| (AF067650) sarcosine dehydrogenase [Rattus norvegicus]
Length = 919
Score = 115 bits (285), Expect = 1e-24
Identities = 97/375 (25%), Positives = 181/375 (47%), Gaps = 51/375 (13%)
Query: 11 HKEHA--KKIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQY 68
HK++A + + + ++ P + I++E LA R A +F++S+ G+ Y G DA K +
Sbjct: 534 HKDYAYSRLLGDEYTFDFPPHHCVIQKECLACRTAAAVFNMSYFGKFYLLGADARKAPDW 593
Query: 69 VTTNDISKPPAISGIYTLVLNERGAIKDETLIFNM--------------GNNEYLMICDS 114
+ + D+++PP S +YT +LN+RG + + + + G+ YL + +
Sbjct: 594 LFSADVNRPPG-STVYTCMLNQRGGTESDLTVSCLAPGAQASPLAPAFEGDGYYLAVGGA 652
Query: 115 DAFEKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDI-NEM 173
A + W T+ Q + ++ + D+ M ++QGP +RD+ +D+ D+ NE
Sbjct: 653 VA---QHNWSHI--NTVLQDQEFRCQLMDCSEDLGMLSIQGPASRDILQDVLDADLSNEA 707
Query: 174 WWFQA-RWVELDGIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERIL 232
+ F + V G + R + GE G+E+++ A+ L V+ ++
Sbjct: 708 FPFSTHQLVRAAGHLVRAIRLSFVGELGWELHVPQAS--------------CLPVYRAVM 753
Query: 233 EEGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFA--IYW 290
G K+G+ AG A D+L +E GY + L S D +PL+A L F +
Sbjct: 754 AAGAKHGLVNAGYRAIDSLSIEKGYRHW-----HADLRSDD----SPLEAGLAFTCKLKT 804
Query: 291 DKDFIGKDALLKQKERGLGRKLVHFKMVDKGIPREGYK-VYANGELIGEVTSGTLSPLLN 349
F+G++AL KQ+ GL R+LV V++ +P G + ++ NG+++G V +N
Sbjct: 805 SVPFLGREALEKQRATGLRRRLVCL-TVEEEVPMFGLEAIWRNGQVVGHVRRADFGFTVN 863
Query: 350 VGIGIAFVKEEYAKP 364
I ++++ P
Sbjct: 864 KTIAYGYIRDPSGGP 878
>gb|AAD53398.2|AF095735_1 (AF095735) sarcosine dehydrogenase [Homo sapiens]
Length = 918
Score = 114 bits (283), Expect = 2e-24
Identities = 93/357 (26%), Positives = 172/357 (48%), Gaps = 49/357 (13%)
Query: 27 PIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPAISGIYTL 86
P + +IK+E LA R A +FD+S+ G+ Y G DA K ++ + D+S+PP S +YT
Sbjct: 551 PPHHDTIKKECLACRGAAAVFDMSYFGKFYLVGLDARKAADWLFSADVSRPPG-STVYTC 609
Query: 87 VLNERGAIKDETLIFNM--------------GNNEYLMICDSDAFEKLYAWFTYLKKTIE 132
+LN RG + + + + G+ YL + + A + ++ T T+
Sbjct: 610 MLNHRGGTESDLTVSRLAPSHQASPLAPAFEGDGYYLAMGGAVA-QHNWSHIT----TVL 664
Query: 133 QFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDI-NEMWWFQA-RWVELDGIKMLL 190
Q K ++ + D+ M ++QGP +R + +++ D+ NE + F + + G +
Sbjct: 665 QDQKSQCQLIDSSEDLGMISIQGPASRAILQEVLDADLSNEAFPFSTHKLLRAAGHLVRA 724
Query: 191 SRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDT 250
R + GE G+E++I A+ + V+ ++ G K+G+ AG A D+
Sbjct: 725 MRLSFVGELGWELHIPKAS--------------CVPVYRAVMAAGAKHGLINAGYRAIDS 770
Query: 251 LRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFA--IYWDKDFIGKDALLKQKERGL 308
L +E GY + + + + +PL+A L F + F+G++AL +Q+ GL
Sbjct: 771 LSIEKGYRHWHADLR---------PDDSPLEAGLAFTCKLKSPVPFLGREALEQQRAAGL 821
Query: 309 GRKLVHFKMVDKGIPREGYK-VYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYAKP 364
R+LV F M DK +P G + ++ NG+++G V ++ I ++ + P
Sbjct: 822 RRRLVCFTMEDK-VPMFGLEAIWRNGQVVGHVRRADFGFAIDKTIAYGYIHDPSGGP 877
>gb|AAD33412.1| (AF129265) unknown [Homo sapiens]
Length = 602
Score = 112 bits (277), Expect = 8e-24
Identities = 90/332 (27%), Positives = 164/332 (49%), Gaps = 49/332 (14%)
Query: 27 PIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPAISGIYTL 86
P + +IK+E LA R A +FD+S+ G+ Y G DA K ++ + D+S+PP S +YT
Sbjct: 271 PPHHDTIKKECLACRGAAAVFDMSYFGKFYLVGLDARKAADWLFSADVSRPPG-STVYTC 329
Query: 87 VLNERGAIKDETLIFNM--------------GNNEYLMICDSDAFEKLYAWFTYLKKTIE 132
+LN G + + + + G+ YL + + A + ++ T T+
Sbjct: 330 MLNHHGGTESDLTVSRLAPSHQASPLAPAFEGDGYYLAVGGAVA-QHNWSHIT----TVL 384
Query: 133 QFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDI-NEMWWFQA-RWVELDGIKMLL 190
Q K ++ + D+ M ++QGP +R + +++ D+ NE + F + + G +
Sbjct: 385 QDQKSQCQLIDSSEDLGMISIQGPASRAILQEVLDADLSNEAFPFSTHKLLRAAGHLVRA 444
Query: 191 SRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDT 250
R + GE G+E++I A+ + V+ ++ G K+G+ AG A D+
Sbjct: 445 MRLSFVGELGWELHIPKAS--------------CVPVYRAVMAAGAKHGLINAGYRAIDS 490
Query: 251 LRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFA--IYWDKDFIGKDALLKQKERGL 308
L +E GY + + + + +PL+A L F + F+G++AL +Q+ GL
Sbjct: 491 LSIEKGYRHWHADLR---------PDDSPLEAGLAFTCKLKSPVPFLGREALEQQRAAGL 541
Query: 309 GRKLVHFKMVDKGIPREGYK-VYANGELIGEV 339
R+LV F M DK +P G + ++ NG+++G V
Sbjct: 542 RRRLVCFTMEDK-VPMFGLEAIWRNGQVVGHV 572
>gb|AAD32214.1| (AF140745) sarcosine dehydrogenase [Homo sapiens]
>gi|5453465|gb|AAD43585.1|AF162428_1 (AF162428)
sarcosine dehydrogenase [Homo sapiens]
Length = 918
Score = 111 bits (275), Expect = 1e-23
Identities = 89/356 (25%), Positives = 167/356 (46%), Gaps = 47/356 (13%)
Query: 27 PIWYSSIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPAISGIYTL 86
P + +IK+E LA R A +FD+S+ G+ Y G DA K ++ + D+S+PP S +YT
Sbjct: 551 PPHHDTIKKECLACRGAAAVFDMSYFGKFYLVGLDARKAADWLFSADVSRPPG-STVYTC 609
Query: 87 VLNERGAIKDETLIFNMG-------------NNEYLMICDSDAFEKLYAWFTYLKKTIEQ 133
+LN G + + + + + Y + + ++ T T+ Q
Sbjct: 610 MLNHXGGTESDLTVSRLAPSHQASPLAPAFEGDGYYLAXGGAVAQHNWSHIT----TVLQ 665
Query: 134 FTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDI-NEMWWFQA-RWVELDGIKMLLS 191
K ++ + D+ M ++QGP +R + +++ D+ NE + F + + G +
Sbjct: 666 DQKSQCQLIDSSEDLGMISIQGPASRAILQEVLDADLSNEAFPFSTHKLLRAAGHLVRAM 725
Query: 192 RSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDTL 251
R + GE G+E++I A+ + V+ ++ G K+G+ AG A D+L
Sbjct: 726 RLSFVGELGWELHIPKAS--------------CVPVYRAVMAAGAKHGLINAGYRAIDSL 771
Query: 252 RLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFA--IYWDKDFIGKDALLKQKERGLG 309
+E GY + + + + +PL+A L F + F+G++AL +Q+ GL
Sbjct: 772 SIEKGYRHWHADLR---------PDDSPLEAGLAFTCKLKSPVPFLGREALEQQRAAGLR 822
Query: 310 RKLVHFKMVDKGIPREGYK-VYANGELIGEVTSGTLSPLLNVGIGIAFVKEEYAKP 364
R+LV F M DK +P G + ++ NG+++G V ++ I ++ + P
Sbjct: 823 RRLVCFTMEDK-VPMFGLEAIWRNGQVVGHVRRADFGFAIDKTIAYGYIHDPSGGP 877
>gb|AAF45943.1| (AE003430) CG3626 gene product [Drosophila melanogaster]
Length = 777
Score = 108 bits (268), Expect = 1e-22
Identities = 102/410 (24%), Positives = 177/410 (42%), Gaps = 64/410 (15%)
Query: 3 KRVHLFDWHKEHAK------KIEEFAGWEMPIWYSSIKEEHLAVRNAVGLFDVSHMGEIY 56
+R + FD +H + +I + + P W+ + E+ A R +G+ D S +
Sbjct: 345 ERPNYFDQQDKHDEFGLPRFRIAQTRTFGKPPWFDHVASEYRACRERIGIADYSSFTKYD 404
Query: 57 F--RGKDALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDS 114
F +G + + LQY+ +ND+ S I+T + N G +++ + + Y+MI +
Sbjct: 405 FWSKGNEVVDLLQYLCSNDVDVAVG-SIIHTGMQNPNGGYENDCSLARLSERHYMMIAPT 463
Query: 115 DAFEKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINEMW 174
+ W ++K + + + + T + GP +R L +L D+
Sbjct: 464 IQQTRSMCW---IRKHMPNHLRAKVNVADVTSMYTAICILGPYSRILLSELTDTDLTPKS 520
Query: 175 WFQARWVELD-----GIKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWE 229
+ ELD GI++L +TGE G+ +YI + E ALHV+
Sbjct: 521 FPSNLCQELDVGLADGIRVL--NITHTGELGYVLYIPN--------------EYALHVYS 564
Query: 230 RILEEGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDE-VTPLQANLEFAI 288
R+ + G+K+ I+ AG A LR+E Y +G D+D TPL+ + +
Sbjct: 565 RLYQAGQKFNIQHAGYYATRALRIEKFYAFWGQ----------DLDTFTTPLECGRSWRV 614
Query: 289 YWDK--DFIGKDALLKQKERGLGRKLVHFKMVDKGIPRE-----GYKVYANGELIGEVTS 341
++K DFIG++ALLKQ+E G+ R V + D + G +Y +G +G T+
Sbjct: 615 KFNKPIDFIGRNALLKQREEGVKRMYVQLLLNDHDHEVDMWCWGGEPIYRDGVYVGMTTT 674
Query: 342 GTLSPLLNVGIGIAFVK------------EEYAKPGIEIEVEIRGARKKA 379
+ + FV+ EY G EVE+ G R +A
Sbjct: 675 TGYGYTFEKQVCLGFVRNFDDEGRELPVTNEYVLSG-HYEVEVAGVRFEA 723
>sp|O87386|SOXA_RHIME SARCOSINE OXIDASE ALPHA SUBUNIT (SARCOSINE OXIDASE SUBUNIT)
Length = 987
Score = 98.7 bits (242), Expect = 1e-19
Identities = 110/407 (27%), Positives = 170/407 (41%), Gaps = 48/407 (11%)
Query: 7 LFDWHKEHAKKIEEFAGWEMPIWY---------SSIKEEHLAVRNAVGLFDVSHMGEIYF 57
L DW K+H E W W+ S++ E L VR GL DVS +G+I
Sbjct: 600 LHDWAKKHGAVFVETGLWYRSSWFPRSGERTWRESVEREVLNVRKNAGLCDVSMLGKIEI 659
Query: 58 RGKDALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAF 117
G DA +FL V N K P Y L+L E G I D+ + N + M +
Sbjct: 660 TGSDAAEFLNRVYCNAFLKLPVGKARYGLMLREDGFIYDDGTTSRLEENRFFMTTTTAYA 719
Query: 118 EKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFAVQGPKARDLARDLFGIDINE--MWW 175
+ + + + + +LD+ + T A A+ GPKAR + + + DI++ +
Sbjct: 720 AGVMNHLEFCAQVL--WPQLDVRLASITDQWAQMAIAGPKARMILQKIVDEDISDAAFPF 777
Query: 176 FQARWVELDG--IKMLLSRSGYTGENGFEVYIEDANPYHPDESKRGEPEKALHVWERILE 233
A+ V L G + L R ++GE +E+ A P ES + + +LE
Sbjct: 778 LAAKEVSLFGGALHGCLFRISFSGELAYEL----AVPAGYGES----------IADALLE 823
Query: 234 EGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQLLSTDIDEVTPLQANLEFAIYWDKD 293
GK +GI P G+ LR+E G+ + NE ++ D+ + A D
Sbjct: 824 AGKDHGIMPYGVETLSVLRIEKGHVTH-NEING-TIVPADLGFGKMVSAG-------KPD 874
Query: 294 FIGKDALLKQKERGLGR-KLVHFKMVD-KGIPREGYKVYANGELI------GEVTSGTLS 345
F+GK L ++ R +LV +D + R G + A G G VTS S
Sbjct: 875 FVGKAMLQREGLTAPDRPQLVGVVPLDPQQSFRSGSHILAKGAAATLENDEGYVTSSAYS 934
Query: 346 PLLNVGIGIAFVKEEYAKPGIEIEV--EIRGARKKAITVTPPFYDPK 390
P + I +A V+ + G E+ V + G A P F+DP+
Sbjct: 935 PHVGSTIALALVRNGRNRHGEEVLVWSGLHGESTPARLCNPVFFDPQ 981
>gb|AAG37965.1| (AC087079) Hypothetical protein Y37E3.u [Caenorhabditis elegans]
Length = 605
Score = 88.5 bits (216), Expect = 1e-16
Identities = 87/371 (23%), Positives = 173/371 (46%), Gaps = 48/371 (12%)
Query: 36 EHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPAISGIYT-LVLNERGAI 94
E+ V N G+ D+S G+I +G DA K + Y + I PA+ I + L+L G I
Sbjct: 264 EYEMVTNKCGVIDLSWKGKIEVKGNDAEKLMDYAIASQI---PALGKISSGLMLTRHGGI 320
Query: 95 KDETLIFNMGNNE--YLMICDSDAFEKLYAWFTYLKKTIEQFTKLDLEIELKTYDIAMFA 152
+IF+ ++++ + + + W L++ + K D+++ + + +A A
Sbjct: 321 LGPMMIFHHDRQRSAFILLTEPERESRDLYW---LRRAAAE-KKFDVQVSIVSEYLASLA 376
Query: 153 VQGPKARDLARDLFGIDINEMWWFQ--ARWVELDGIKMLLSRSGY-TGENGFEVYIEDAN 209
+ GPK+R++ L D+++ + Q R + L + ++ +RS TG+ +E++
Sbjct: 377 LVGPKSREVLSALTKSDVSDEGFPQKSTRMIRLGPVGVVCARSSTSTGQLSYELF----- 431
Query: 210 PYHPDESKRGEPEKALHVWERILEEGKKYGIKPAGLGARDTLRLEAGYTLYGNETKELQL 269
R E K ++ ++ G+++GI G A + +RLE GY ++G KEL L
Sbjct: 432 ------HNRAETAK---LYNAVMSAGREHGIVNFGQAALNMMRLEHGYKIWG---KELTL 479
Query: 270 LSTDIDEVTPLQANLEFAIYWD-KDFIGKDALLKQKERGLGRKLVHFK--------MVDK 320
+ P + + + ++ K+FIG+++ L+ K++ R+L + D+
Sbjct: 480 ------DTNPFECGIGGLVDFEKKEFIGRESALELKKKDFDRRLALITFDTEEGCVLDDR 533
Query: 321 GIPREGYKVYANGE--LIGEVTSGTLSPLLNVGIGIAFVKEEYAKPGIEIEVEIRGARKK 378
+P + +G+ +G++TSG + L I A++ K + V+I R
Sbjct: 534 YVPSGNEVIRIDGKEARVGQITSGAYNVRLQKPIAFAWIDNAVGK-NERLVVDIGDKRLF 592
Query: 379 AITVTPPFYDP 389
A ++ P P
Sbjct: 593 ATSLETPTIPP 603
>gb|AAC31611.1| (AF082220) aminomethyltransferase [Bartonella grahamii]
Length = 189
Score = 85.0 bits (207), Expect = 1e-15
Identities = 56/187 (29%), Positives = 95/187 (49%), Gaps = 13/187 (6%)
Query: 21 FAGWEMPIWYS-SIKEEHLAVRNAVGLFDVSHMGEIYFRGKDALKFLQYVTTNDISKPPA 79
FA W MP+ Y + +EHL R GLFD+SHM I G A++FL Y D +
Sbjct: 5 FARWNMPLTYPLGVLKEHLHTRAHAGLFDISHMKLIAVEGAQAVEFLSYAFPIDAAALKI 64
Query: 80 ISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDSDAFEKLYAWFTYLKKTIEQFTKLDL 139
Y +LN + I D+ +I + + ++++ ++ A F L+K +D
Sbjct: 65 GHSRYNYLLNGQAGILDDLIITRLEESRFMLVANAG---NAQADFVELEK-----RAVDF 116
Query: 140 EIELKTYDIAMFAVQGPKARDLARDLFGIDINEMWWFQARWVELDGIKMLLSRSGYTGEN 199
E ++ + + A+QGP+A + D + NE+++ Q + D ++RSGYTGE+
Sbjct: 117 ECQVIALERVLLALQGPEAAAVLADA-DLPGNELFFMQGFEPQQD---WFITRSGYTGED 172
Query: 200 GFEVYIE 206
GFE+ ++
Sbjct: 173 GFEIALQ 179
>gb|AAC04876.1| (AF047332) glycine cleavage system T-protein [Drosophila
heteroneura]
Length = 148
Score = 69.1 bits (166), Expect = 9e-11
Identities = 37/114 (32%), Positives = 62/114 (53%), Gaps = 2/114 (1%)
Query: 3 KRVHLFDWHKEHAKKIEEFAGWEMPIWYS--SIKEEHLAVRNAVGLFDVSHMGEIYFRGK 60
+R L+D+H + KI F G+ +P+ Y+ SI H R +FDVSHM + Y RG
Sbjct: 34 ERTALYDFHARKSGKIVNFGGYALPVQYADQSIIASHNYTRQVGSIFDVSHMLQTYVRGN 93
Query: 61 DALKFLQYVTTNDISKPPAISGIYTLVLNERGAIKDETLIFNMGNNEYLMICDS 114
DA L+ ++T DI +G T+ NE+G I D+ ++ + + E ++ ++
Sbjct: 94 DAAACLESISTADILGMLPGAGSLTVFTNEQGCILDDLIVNKVSDKELYVVSNA 147
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.140 0.420
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 157564098
Number of Sequences: 2977
Number of extensions: 7061528
Number of successful extensions: 17134
Number of sequences better than 1.0e-10: 61
Number of HSP's better than 0.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 47
Number of HSP's that attempted gapping in prelim test: 16878
Number of HSP's gapped (non-prelim): 66
length of query: 398
length of database: 189,106,746
effective HSP length: 54
effective length of query: 344
effective length of database: 156,788,448
effective search space: 53935226112
effective search space used: 53935226112
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)