BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1645 (thiE) DE:thiamine phosphate pyrophosphorylase (thiE)
(207 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75087 thiamin phosphate pyrophosphorylase (thie) PAB1645 -... 394 e-109
sp|O58878|THIE_PYRHO PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLA... 346 2e-94
pir||F71057 probable thiamin biosynthetic bifunctional protein -... 272 3e-72
gi|11499656 thiamine phosphate pyrophosphorylase (thiE) [Archaeo... 192 2e-48
sp|O66833|THIE_AQUAE PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLA... 161 8e-39
pir||T07834 hydroxymethylpyrimidine kinase (EC 2.7.1.49) / thiam... 160 1e-38
dbj|BAB05150.1| (AP001512) thiamine phosphate pyrophosphorylase ... 156 1e-37
gb|AAB72162.1| (AF000657) probable thiamin biosynthetic enzyme [... 146 2e-34
sp|P72965|THIE_SYNY3 PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLA... 143 1e-33
sp|O67378|YD66_AQUAE HYPOTHETICAL PROTEIN AQ_1366 >gi|7451725|pi... 143 1e-33
sp|P40386|THI4_SCHPO PROBABLE THIAMINE BIOSYNTHETIC BIFUNCTIONAL... 137 1e-31
emb|CAC12401.1| (AL445067) thiamine phosphate pyrophosphorylase ... 137 1e-31
sp|P39594|THIE_BACSU THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP P... 132 4e-30
pdb|2TPS|B Chain B, Thiamin Phosphate Synthase >gi|4699824|pdb|2... 132 4e-30
pir||D81311 thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) Cj1... 128 5e-29
gi|6325042 TMP pyrophosphorylase, hydroxyethylthiazole kinase; T... 120 9e-27
sp|P71350|THIE_HAEIN THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP P... 114 9e-25
pir||A75614 thiamin-phosphate pyrophosphorylase - Deinococcus ra... 113 2e-24
gb|AAF25544.1|AF109218_4 (AF109218) ThiE [Staphylococcus carnosus] 104 6e-22
sp|O25514|THIE_HELPY PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLA... 103 1e-21
sp|Q9ZL01|THIE_HELPJ PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLA... 100 1e-20
sp|P96260|THIE_MYCTU PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLA... 99 4e-20
gb|AAF33508.1| (AF170176) 90% identity over 211 amino acids with... 94 9e-19
sp|P25053|TENI_BACSU REGULATORY PROTEIN TENI >gi|73041|pir||XMBS... 94 1e-18
gb|AAD56925.1|AF180145_17 (AF180145) thiamine-phosphate pyrophos... 92 4e-18
sp|Q9ZBL5|THIE_MYCLE PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLA... 90 1e-17
sp|P30137|THIE_ECOLI THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP P... 87 1e-16
pir||A82814 thiamin-phosphate pyrophosphorylase XF0378 [imported... 87 1e-16
pir||A82369 thiamin-phosphate pyrophosphorylase VC0062 [imported... 86 2e-16
pir||T34974 probable thiamin phosphate pyrophosphorylase - Strep... 86 3e-16
pir||E82032 thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) NMA... 85 4e-16
pir||F83149 thiamin-phosphate pyrophosphorylase PA3976 [imported... 84 1e-15
pir||A81011 thiamin-phosphate pyrophosphorylase NMB2069 [importe... 83 2e-15
pir||H81306 probable transferase Cj1043c [imported] - Campylobac... 71 7e-12
pir||E82720 bifunctional DGTP-pyrophosphohydrolase/thiamin phosp... 70 2e-11
>pir||D75087 thiamin phosphate pyrophosphorylase (thie) PAB1645 - Pyrococcus
abyssi (strain Orsay) >gi|5458513|emb|CAB50001.1|
(AJ248286) thiamine phosphate pyrophosphorylase (thiE)
[Pyrococcus abyssi]
Length = 207
Score = 394 bits (1002), Expect = e-109
Identities = 207/207 (100%), Positives = 207/207 (100%)
Query: 1 MGFREKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQE 60
MGFREKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQE
Sbjct: 1 MGFREKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQE 60
Query: 61 YDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKG 120
YDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKG
Sbjct: 61 YDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKG 120
Query: 121 ADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIA 180
ADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIA
Sbjct: 121 ADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIA 180
Query: 181 VVSAVMGASDVKKATEELRKIIEEVLG 207
VVSAVMGASDVKKATEELRKIIEEVLG
Sbjct: 181 VVSAVMGASDVKKATEELRKIIEEVLG 207
>sp|O58878|THIE_PYRHO PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE)
Length = 207
Score = 346 bits (877), Expect = 2e-94
Identities = 175/207 (84%), Positives = 193/207 (92%)
Query: 1 MGFREKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQE 60
M F+EKLKLY+ITDRRLKPE+ SV+QALEGGATSIQLRIK+A T+EMYE+GKEIR+LT E
Sbjct: 1 MNFKEKLKLYIITDRRLKPEIASVKQALEGGATSIQLRIKNAPTREMYEIGKEIRKLTNE 60
Query: 61 YDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKG 120
Y ALFFVDDRIDVALAVNADGVQLGP+DMPIE+AREIAPNLIIGASVYSLEEALEAE KG
Sbjct: 61 YGALFFVDDRIDVALAVNADGVQLGPDDMPIEIAREIAPNLIIGASVYSLEEALEAEMKG 120
Query: 121 ADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIA 180
ADYLGAGSVFPT+TK+DV+VI IEGLREIV AV IPVVAIGGIN+ENV++VL GVDGIA
Sbjct: 121 ADYLGAGSVFPTQTKKDVKVIGIEGLREIVNAVKIPVVAIGGINLENVREVLLTGVDGIA 180
Query: 181 VVSAVMGASDVKKATEELRKIIEEVLG 207
VVSAVMG DVK+ATE LR+IIEEVLG
Sbjct: 181 VVSAVMGTEDVKRATEGLRRIIEEVLG 207
>pir||F71057 probable thiamin biosynthetic bifunctional protein - Pyrococcus
horikoshii >gi|3257573|dbj|BAA30256.1| (AP000005) 161aa
long hypothetical thiamine biosynthetic bifunctional
protein [Pyrococcus horikoshii]
Length = 161
Score = 272 bits (687), Expect = 3e-72
Identities = 138/161 (85%), Positives = 150/161 (92%)
Query: 47 MYEVGKEIRRLTQEYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGAS 106
MYE+GKEIR+LT EY ALFFVDDRIDVALAVNADGVQLGP+DMPIE+AREIAPNLIIGAS
Sbjct: 1 MYEIGKEIRKLTNEYGALFFVDDRIDVALAVNADGVQLGPDDMPIEIAREIAPNLIIGAS 60
Query: 107 VYSLEEALEAEKKGADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVE 166
VYSLEEALEAE KGADYLGAGSVFPT+TK+DV+VI IEGLREIV AV IPVVAIGGIN+E
Sbjct: 61 VYSLEEALEAEMKGADYLGAGSVFPTQTKKDVKVIGIEGLREIVNAVKIPVVAIGGINLE 120
Query: 167 NVKQVLSAGVDGIAVVSAVMGASDVKKATEELRKIIEEVLG 207
NV++VL GVDGIAVVSAVMG DVK+ATE LR+IIEEVLG
Sbjct: 121 NVREVLLTGVDGIAVVSAVMGTEDVKRATEGLRRIIEEVLG 161
>gi|11499656 thiamine phosphate pyrophosphorylase (thiE) [Archaeoglobus
fulgidus] >gi|7388297|sp|O28205|THIE_ARCFU PROBABLE
THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE) >gi|7451723|pir||A69509 thiamin phosphate
pyrophosphorylase (thiE) homolog - Archaeoglobus
fulgidus >gi|2648452|gb|AAB89175.1| (AE000960) thiamine
phosphate pyrophosphorylase (thiE) [Archaeoglobus
fulgidus]
Length = 210
Score = 192 bits (484), Expect = 2e-48
Identities = 107/198 (54%), Positives = 136/198 (68%), Gaps = 4/198 (2%)
Query: 7 LKLYVITDRRL-KPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALF 65
L +Y ITD + + AL G +IQ R K STK MYE+GK +R LT++YDALF
Sbjct: 8 LSVYFITDSEFGRTHEELAEMALRAGVRAIQFREKKLSTKRMYEIGKRLRALTRDYDALF 67
Query: 66 FVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKGADYLG 125
FV+DRIDVALAV+ADGV +G +DMP AREI P IG S ++EEA + +++ ADYLG
Sbjct: 68 FVNDRIDVALAVDADGVHIGQDDMPAFAAREIFPG-YIGVSAGNVEEA-KKDERFADYLG 125
Query: 126 AGSVFPTKTKRDV-RVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVSA 184
G VFPTKTK D I IEGLR IVE+VS+PVVAIG IN +N +VL GV GIAV+SA
Sbjct: 126 VGPVFPTKTKEDAGEAIGIEGLRRIVESVSVPVVAIGSINKQNAIEVLKTGVAGIAVISA 185
Query: 185 VMGASDVKKATEELRKII 202
+ A D ++A EL +++
Sbjct: 186 IAAADDPERAARELVELV 203
>sp|O66833|THIE_AQUAE PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE) >gi|7451724|pir||E70350 probable thiamin
phosphate synthase 1 - Aquifex aeolicus
>gi|2983186|gb|AAC06786.1| (AE000695) thiamine phosphate
synthase [Aquifex aeolicus]
Length = 215
Score = 161 bits (402), Expect = 8e-39
Identities = 83/204 (40%), Positives = 134/204 (65%), Gaps = 4/204 (1%)
Query: 7 LKLYVITDRRLKPE---VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDA 63
L +Y+ITD + + ++ QAL+GG T++Q R ++ +T++MYE ++R LT++Y+A
Sbjct: 3 LTIYLITDDKYFKDRDLFNTIEQALQGGVTAVQYRFENKTTRQMYEELVKLRELTRKYNA 62
Query: 64 LFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPN-LIIGASVYSLEEALEAEKKGAD 122
V+DR+D+ALAV ADGV +G +D+P EV R+I + + IG + SLEE A++ D
Sbjct: 63 DLVVNDRVDLALAVEADGVHIGKDDLPPEVVRKIVGDKMYIGYTANSLEEVKRAQELPVD 122
Query: 123 YLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVV 182
Y+G GS++ T TK + +++ +E L+E V+ P+V IGGI V +V+ AG IAV
Sbjct: 123 YIGFGSIYHTSTKENYKLVGVEALKEAVKISQKPIVCIGGIMPYRVPEVVRAGCRNIAVS 182
Query: 183 SAVMGASDVKKATEELRKIIEEVL 206
S ++G +DVKKA E +++ +E L
Sbjct: 183 SGILGFADVKKAAESIKRAYQETL 206
>pir||T07834 hydroxymethylpyrimidine kinase (EC 2.7.1.49) / thiamin-phosphate
pyrophosphorylase (EC 2.5.1.3) - rape
>gi|2746079|gb|AAC31298.1| (AF015310) BTH1 [Brassica
napus]
Length = 523
Score = 160 bits (400), Expect = 1e-38
Identities = 87/209 (41%), Positives = 130/209 (61%), Gaps = 9/209 (4%)
Query: 5 EKLKLYVITDRRLKPE-----VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQ 59
+ L LY +TD R+ + V +V+ A+EGGAT IQLR K+A T+E E K + +
Sbjct: 310 DDLFLYAVTDSRMNKKWNRSIVDAVKAAIEGGATIIQLREKEAETREFLEEAKSCVDICR 369
Query: 60 EYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALEAEK 118
++DR D+A+A++ADGV +G DMP+++ R + P+ IIG S + E+A +A K
Sbjct: 370 SNGVCLLINDRFDIAIALDADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTQEQAHQAWK 429
Query: 119 KGADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAG--- 175
GADY+G+G VFPT TK + R I ++GLRE+ +A +PVVAIGGI + N + V+ G
Sbjct: 430 DGADYIGSGGVFPTNTKANNRTIGLDGLREVCKASKLPVVAIGGIGISNAESVMRIGEPN 489
Query: 176 VDGIAVVSAVMGASDVKKATEELRKIIEE 204
+ G+AVVSA+ V ++L K + E
Sbjct: 490 LKGVAVVSALFDQECVLTQAKKLHKTLTE 518
>dbj|BAB05150.1| (AP001512) thiamine phosphate pyrophosphorylase [Bacillus
halodurans]
Length = 211
Score = 156 bits (391), Expect = 1e-37
Identities = 85/202 (42%), Positives = 126/202 (62%), Gaps = 5/202 (2%)
Query: 8 KLYVITDRRLKPEVKS---VRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDAL 64
+LY IT P+ + +A+ GG IQLR K + +E+ E + ++ L ++YD
Sbjct: 5 RLYAITGEEFHPDRSLQAVMEEAILGGVDIIQLRDKKSHKREVLEKARVLQALAKKYDIP 64
Query: 65 FFVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALEAEKKGADY 123
++D IDVALAV+ADG+ LG +D+P+ AR+I + IIG S + +EEA EAEK GADY
Sbjct: 65 LIINDHIDVALAVDADGIHLGQDDLPLSEARKIMGRDKIIGISTHKIEEAREAEKGGADY 124
Query: 124 LGAGSVFPTKTKRD-VRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVV 182
+G G +F TK+K D V + ++++ +SIP VAIGGI + NV+QVL AG I ++
Sbjct: 125 IGVGPIFETKSKEDVVDPVTTAYIQQVAHEISIPFVAIGGIKLHNVEQVLDAGATRICMI 184
Query: 183 SAVMGASDVKKATEELRKIIEE 204
S ++GA DVK E I+E+
Sbjct: 185 SEIVGAEDVKGTCEVFSTILEQ 206
>gb|AAB72162.1| (AF000657) probable thiamin biosynthetic enzyme [Arabidopsis
thaliana]
Length = 525
Score = 146 bits (364), Expect = 2e-34
Identities = 84/209 (40%), Positives = 126/209 (60%), Gaps = 14/209 (6%)
Query: 5 EKLKLYVITDRRLKPE-----VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQ 59
+ L LY +TD R+ + V +++ A+EGGAT IQLR E K + +
Sbjct: 317 DDLFLYAVTDSRMNKKWNRSIVDALKAAIEGGATIIQLRFD-----HFLEEAKACIDICR 371
Query: 60 EYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALEAEK 118
+ ++DRID+ALA +ADGV +G DMP+++ R + P+ IIG S + E+A +A K
Sbjct: 372 SHGVSLLINDRIDIALACDADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWK 431
Query: 119 KGADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVEN---VKQVLSAG 175
GADY+G+G VFPT TK + R I ++GL+E+ EA +PVVAIGGI + N V Q+ +
Sbjct: 432 DGADYIGSGGVFPTNTKANNRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPN 491
Query: 176 VDGIAVVSAVMGASDVKKATEELRKIIEE 204
+ G+AVVSA+ V ++L K ++E
Sbjct: 492 LKGVAVVSALFDQDCVLTQAKKLHKTLKE 520
>sp|P72965|THIE_SYNY3 PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE) >gi|7427960|pir||S74943 thiamin biosynthetic
bifunctional protein - Synechocystis sp. (strain PCC
6803) >gi|1652058|dbj|BAA16983.1| (D90902) thiamin
biosynthetic bifunctional enzyme [Synechocystis sp.]
Length = 343
Score = 143 bits (357), Expect = 1e-33
Identities = 78/195 (40%), Positives = 126/195 (64%), Gaps = 2/195 (1%)
Query: 5 EKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDAL 64
++ LY++T + + + +V AL+GG +Q R KDA + ++ K++R L ++Y+AL
Sbjct: 141 QQCSLYLVTSPQ-ENLLATVEAALQGGLKLVQYRDKDAEDQLRWQRAKDLRELCRQYEAL 199
Query: 65 FFVDDRIDVALAVNADGVQLGPEDMPIEVARE-IAPNLIIGASVYSLEEALEAEKKGADY 123
F V+DR+D+ALAV+ADGV LG +D+PI VAR+ + P+ IIG S + EE +A +GADY
Sbjct: 200 FLVNDRVDLALAVDADGVHLGQQDLPIAVARQLLGPDKIIGRSTTNPEEMAKAIAEGADY 259
Query: 124 LGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVS 183
+G G V+ T TK + +E ++ V +P AIGGI+ EN+ +V+ AG +A+V
Sbjct: 260 IGVGPVYATPTKAGKKPAGLEYVQYAVTNSPVPWFAIGGIDGENLGEVMEAGATQVAIVR 319
Query: 184 AVMGASDVKKATEEL 198
A+M ++ +AT +L
Sbjct: 320 AIMETTNPTQATAQL 334
>sp|O67378|YD66_AQUAE HYPOTHETICAL PROTEIN AQ_1366 >gi|7451725|pir||G70418 probable
thiamin phosphate synthase 2 - Aquifex aeolicus
>gi|2983767|gb|AAC07326.1| (AE000736) thiamine phosphate
synthase [Aquifex aeolicus]
Length = 186
Score = 143 bits (357), Expect = 1e-33
Identities = 73/178 (41%), Positives = 113/178 (63%), Gaps = 2/178 (1%)
Query: 8 KLYVITDRRLKPE--VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALF 65
+LY ITDR+ E ++++ + L+ G +QLR KD +E+Y++ KE+R LT++Y AL
Sbjct: 5 RLYAITDRKKYGENFLETLEKILKKGVRMVQLREKDLKDRELYKLAKEVRALTKKYKALL 64
Query: 66 FVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKGADYLG 125
+++R D+ALAV ADGV L + P V + + PN I+G S +SLE A AEK+GAD++
Sbjct: 65 LINERFDIALAVEADGVHLPEQSFPPSVVKRVNPNFIVGFSAHSLESAKYAEKEGADFIT 124
Query: 126 AGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVS 183
+F T + + + I ++ L+E+ E V+IPV A+GGI E +K G GIA +S
Sbjct: 125 LSPIFKTSSHPEAQPIGLKTLKEVSEKVNIPVYALGGITWEKIKVCYKNGAYGIAGIS 182
>sp|P40386|THI4_SCHPO PROBABLE THIAMINE BIOSYNTHETIC BIFUNCTIONAL ENZYME [INCLUDES:
THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE); HYDROXYETHYLTHIAZOLE
KINASE (4-METHYL-5-BETA-HYDROXYETHYLTHIAZOLE KINASE)
(THZ KINASE) (TH KINASE)] >gi|629908|pir||S44183
thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) /
hydroxyethylthiazole kinase (EC 2.7.1.50) thi4 - fission
yeast (Schizosaccharomyces pombe)
>gi|473357|emb|CAA55402.1| (X78824) thi4
[Schizosaccharomyces pombe] >gi|2388929|emb|CAB11664.1|
(Z98977) probable thiamine biosynthetic bifunctional
enzyme [includes:thiamine-phosphate pyrophosphorylase]
[Schizosaccharomyces pombe]
Length = 518
Score = 137 bits (341), Expect = 1e-31
Identities = 87/212 (41%), Positives = 128/212 (60%), Gaps = 19/212 (8%)
Query: 9 LYVITDRRLKPEVKSVRQALE----GGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDAL 64
LY++T L ++ + +E GG T +Q R KD STK E K + + ++YD
Sbjct: 9 LYLVTSSSLIAPGSTIERQVEEGILGGVTLVQHREKDISTKCFVERAKRLSEICKKYDVP 68
Query: 65 FFVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALEAEKKGADY 123
F ++DRIDVALAV ADGV +G +DM +AR+I + IIG S ++EE +A GADY
Sbjct: 69 FLINDRIDVALAVGADGVHIGQDDMDCALARKILGDDAIIGVSTNNIEEIEKAAADGADY 128
Query: 124 LGAGSVFPTKTKRDV--RVIRIEGLREIVEAVS-----IPVVAIGGINVENVKQVL---- 172
+G GS++ T TK DV R+I I GLR+I+E VS + VAI G+N N+++V+
Sbjct: 129 VGIGSIYETNTK-DVKDRLIGITGLRKILEHVSKMHCQLGTVAIAGLNSSNIQRVIYLSE 187
Query: 173 --SAGVDGIAVVSAVMGASDVKKATEELRKII 202
+DGIA+VSA+M + ++ +ELR +I
Sbjct: 188 ANGKRIDGIALVSAIMCSITPRETAKELRNLI 219
>emb|CAC12401.1| (AL445067) thiamine phosphate pyrophosphorylase (yeast THI6)
related protein [Thermoplasma acidophilum]
Length = 213
Score = 137 bits (341), Expect = 1e-31
Identities = 74/192 (38%), Positives = 110/192 (56%), Gaps = 1/192 (0%)
Query: 9 LYVIT-DRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALFFV 67
LY++T D K + +A+ GG +Q R K +V ++++++ YD LFF+
Sbjct: 11 LYLVTEDYSRKNFFNIIEEAIIGGVDIVQYRDKSNPRSVRLDVARKVKQICNRYDILFFI 70
Query: 68 DDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKGADYLGAG 127
DD + +A+ V ADGV +G +DMP+ AR I LI ++ E A+ AEK GADY+ G
Sbjct: 71 DDDVQLAIEVQADGVHIGKDDMPLPDARRIFDGLIGYSTYGDREMAIFAEKNGADYVAFG 130
Query: 128 SVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVSAVMG 187
F T TK+D V I L I + + IPV IGGIN+ N++ G+DG+AVVSA+
Sbjct: 131 PFFHTDTKKDADVYDIHVLEGIHKYIRIPVFVIGGINISNIRTFSGYGIDGVAVVSAIFS 190
Query: 188 ASDVKKATEELR 199
D ++A EL+
Sbjct: 191 DPDPERAARELK 202
>sp|P39594|THIE_BACSU THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP PYROPHOSPHORYLASE)
(TMP-PPASE) (THIAMINE-PHOSPHATE SYNTHASE)
>gi|629033|pir||S39681 thiamin-phosphate
pyrophosphorylase (EC 2.5.1.3) thiC - Bacillus subtilis
>gi|413950|emb|CAA51582.1| (X73124) ipa-26d [Bacillus
subtilis] >gi|2636364|emb|CAB15855.1| (Z99123)
thiamine-phosphate pyrophosphorylase [Bacillus subtilis]
Length = 222
Score = 132 bits (328), Expect = 4e-30
Identities = 74/207 (35%), Positives = 124/207 (59%), Gaps = 7/207 (3%)
Query: 4 REKLKLYVI--TDRRLKPEVKSVRQALEGGATSIQLRIK--DASTKEM-YEVGKEIRRLT 58
+E L +Y I ++ V V++AL+GGAT Q R K DA T E + ++ +
Sbjct: 10 KELLSVYFIMGSNNTKADPVTVVQKALKGGATLYQFREKGGDALTGEARIKFAEKAQAAC 69
Query: 59 QEYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEK 118
+E F V+D +++AL + ADG+ +G ED + R ++I+G S +++ E +AE+
Sbjct: 70 REAGVPFIVNDDVELALNLKADGIHIGQEDANAKEVRAAIGDMILGVSAHTMSEVKQAEE 129
Query: 119 KGADYLGAGSVFPTKTKRDVRVIRIEGLREIV--EAVSIPVVAIGGINVENVKQVLSAGV 176
GADY+G G ++PT+TK+D R ++ L E V + +SIP+V IGGI ++N V+ AG
Sbjct: 130 DGADYVGLGPIYPTETKKDTRAVQGVSLIEAVRRQGISIPIVGIGGITIDNAAPVIQAGA 189
Query: 177 DGIAVVSAVMGASDVKKATEELRKIIE 203
DG++++SA+ A D + A + R+ I+
Sbjct: 190 DGVSMISAISQAEDPESAARKFREEIQ 216
>pdb|2TPS|B Chain B, Thiamin Phosphate Synthase >gi|4699824|pdb|2TPS|A Chain A,
Thiamin Phosphate Synthase
Length = 227
Score = 132 bits (328), Expect = 4e-30
Identities = 74/207 (35%), Positives = 124/207 (59%), Gaps = 7/207 (3%)
Query: 4 REKLKLYVI--TDRRLKPEVKSVRQALEGGATSIQLRIK--DASTKEM-YEVGKEIRRLT 58
+E L +Y I ++ V V++AL+GGAT Q R K DA T E + ++ +
Sbjct: 15 KELLSVYFIMGSNNTKADPVTVVQKALKGGATLYQFREKGGDALTGEARIKFAEKAQAAC 74
Query: 59 QEYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEK 118
+E F V+D +++AL + ADG+ +G ED + R ++I+G S +++ E +AE+
Sbjct: 75 REAGVPFIVNDDVELALNLKADGIHIGQEDANAKEVRAAIGDMILGVSAHTMSEVKQAEE 134
Query: 119 KGADYLGAGSVFPTKTKRDVRVIRIEGLREIV--EAVSIPVVAIGGINVENVKQVLSAGV 176
GADY+G G ++PT+TK+D R ++ L E V + +SIP+V IGGI ++N V+ AG
Sbjct: 135 DGADYVGLGPIYPTETKKDTRAVQGVSLIEAVRRQGISIPIVGIGGITIDNAAPVIQAGA 194
Query: 177 DGIAVVSAVMGASDVKKATEELRKIIE 203
DG++++SA+ A D + A + R+ I+
Sbjct: 195 DGVSMISAISQAEDPESAARKFREEIQ 221
>pir||D81311 thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) Cj1081c [imported]
- Campylobacter jejuni (strain NCTC 11168)
>gi|6968515|emb|CAB73336.1| (AL139077) thiamin-phosphate
pyrophosphorylase [Campylobacter jejuni]
Length = 210
Score = 128 bits (318), Expect = 5e-29
Identities = 77/204 (37%), Positives = 129/204 (62%), Gaps = 7/204 (3%)
Query: 7 LKLYVITDRRLKPE---VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDA 63
L LY++ + K E + ++ A++GG + IQLR K+ + +E Y++G ++++L + Y
Sbjct: 7 LSLYLVATKGSKSEECFLNTLENAIKGGVSIIQLREKELNAREFYKLGLKVQKLCKSYKI 66
Query: 64 LFFVDDRIDVALAVNADGVQLGPEDMPIEVARE-IAPNLIIGASVYSLEEALEAEKKGAD 122
F ++DR+D+ALA++ADGV LG ED+ ++AR+ + IIG S+ LE+ LE +G +
Sbjct: 67 PFLINDRVDIALALDADGVHLGQEDLEAKLARKLLGDEKIIGLSLKKLEQ-LEF-IQGVN 124
Query: 123 YLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVV 182
YLG G++ T TK + ++ +E L +I + I VVAIGGI+ E + ++ + G+AVV
Sbjct: 125 YLGCGAIKATPTK-ESSLLSLELLSQICDKSPIGVVAIGGIDKEALVELKGINLSGVAVV 183
Query: 183 SAVMGASDVKKATEELRKIIEEVL 206
A+M A D A +EL++ I E L
Sbjct: 184 RAIMDAKDAFLAAKELKRKIYENL 207
>gi|6325042 TMP pyrophosphorylase, hydroxyethylthiazole kinase; Thi6p
[Saccharomyces cerevisiae]
>gi|1174673|sp|P41835|THI6_YEAST THIAMINE BIOSYNTHETIC
BIFUNCTIONAL ENZYME [INCLUDES: THIAMINE-PHOSPHATE
PYROPHOSPHORYLASE (TMP PYROPHOSPHORYLASE) (TMP-PPASE);
HYDROXYETHYLTHIAZOLE KINASE
(4-METHYL-5-BETA-HYDROXYETHYLTHIAZOLE KINASE) (THZ
KINASE) (TH KINASE)] >gi|1078413|pir||A55145
thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) /
hydroxyethylthiazole kinase (EC 2.7.1.50) THI6 - yeast
(Saccharomyces cerevisiae) >gi|633125|dbj|BAA06703.1|
(D31908) thiamin biosynthetic bifunctional enzyme
[Saccharomyces cerevisiae] >gi|1370444|emb|CAA97929.1|
(Z73570) ORF YPL214c [Saccharomyces cerevisiae]
Length = 540
Score = 120 bits (299), Expect = 9e-27
Identities = 74/219 (33%), Positives = 120/219 (54%), Gaps = 24/219 (10%)
Query: 9 LYVITDRRLKPE----VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDAL 64
LY++TD + P V L+ G T +Q+R KD TK E++++ ++Y+
Sbjct: 12 LYLVTDSTMLPPGTTLCSQVEAGLKNGVTLVQIREKDIETKNFVAEALEVQKICKKYNVP 71
Query: 65 FFVDDRIDVALAVNADGVQLGPEDMPIEVARE-IAPNLIIGASVYSLEEALEAEKKG--- 120
++DRIDVA+A++ADGV +G +DMPI + R+ + P+ I+G SV E K G
Sbjct: 72 LIINDRIDVAMAIDADGVHVGQDDMPIPMVRKLLGPSKILGWSVGKPSEVETLAKWGPDM 131
Query: 121 ADYLGAGSVFPTKTKRDVR--------VIRIEGLREIVEAVSIPVVAIGGINVENVKQVL 172
DY+G G++FPT TK++ + I I E +A V IGG++ +N+++VL
Sbjct: 132 VDYIGVGTLFPTSTKKNPKKSPMGPQGAIAILDALEEFKATWCRTVGIGGLHPDNIQRVL 191
Query: 173 --------SAGVDGIAVVSAVMGASDVKKATEELRKIIE 203
+DGI++VS +M A D AT+ LR +++
Sbjct: 192 CQCVASNGKRSLDGISLVSDIMAAPDACAATKRLRGLLD 230
>sp|P71350|THIE_HAEIN THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP PYROPHOSPHORYLASE)
(TMP-PPASE) (THIAMINE-PHOSPHATE SYNTHASE)
>gi|1074397|pir||A64152 probable thiamin-phosphate
pyrophosphorylase (EC 2.5.1.3) - Haemophilus influenzae
(strain Rd KW20) >gi|1573391|gb|AAC22075.1| (U32725)
thiamin-phosphate pyrophosphorylase [Haemophilus
influenzae Rd]
Length = 226
Score = 114 bits (282), Expect = 9e-25
Identities = 72/192 (37%), Positives = 110/192 (56%), Gaps = 13/192 (6%)
Query: 24 VRQALEGGATSIQLRIKDASTKEMYEVGKEI-----RRLTQEYDALFFVDDRIDVALAVN 78
++QALEGG T Q R K + E ++ R L +EY F VDD +D+AL +
Sbjct: 34 LKQALEGGITCFQFRDKGKFSLEHTPSAQKALAINCRDLCREYGVPFIVDDNVDLALEIE 93
Query: 79 ADGVQLGPEDMPI-EVAREIAPNLIIGASVYSLEEALEAEKKGA-DYLGAGSVFPTKTKR 136
ADG+ +G DMP+ E+ + LIIG SV L+EA E DY G G +FPT++K
Sbjct: 94 ADGIHVGQSDMPVQEIRAKTDKPLIIGWSVNRLDEAKIGENLAEIDYFGIGPIFPTQSKE 153
Query: 137 DVR----VIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVSAVMGASDVK 192
+ + + I+ LR ++ P+VAIGG+ + +VK + G DG+AV++A+ A +V+
Sbjct: 154 NPKPTLGMAFIQTLRNA--GITKPLVAIGGVKLAHVKTLREFGADGVAVITAITHADNVQ 211
Query: 193 KATEELRKIIEE 204
AT+ LR+ +E
Sbjct: 212 AATKALREASDE 223
>pir||A75614 thiamin-phosphate pyrophosphorylase - Deinococcus radiodurans
(strain R1) >gi|6460491|gb|AAF12197.1|AE001862_23
(AE001862) thiamin-phosphate pyrophosphorylase
[Deinococcus radiodurans]
Length = 280
Score = 113 bits (279), Expect = 2e-24
Identities = 72/188 (38%), Positives = 100/188 (52%), Gaps = 6/188 (3%)
Query: 21 VKSVRQALEGGATSIQLRIKDAS-----TKEMYEVGKEIRRLTQEYDALFFVDDRIDVAL 75
++ V AL GG ++QLR K S + + + +R L + FFV+DR+DVAL
Sbjct: 89 LRRVEDALAGGVDTLQLRCKADSGQYGEARPYIALAERLRDLAHARNVPFFVNDRVDVAL 148
Query: 76 AVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKGADYLGAGSVFPTKTK 135
A ADGV LG D+P+E AR +AP L +G S + E+A A + Y G V T TK
Sbjct: 149 AAGADGVHLGQNDLPVEWARLMAPGLRVGRSTHRPEDAARALAEAPAYFATGPVHATPTK 208
Query: 136 RDVRVIRIEGLREIVE-AVSIPVVAIGGINVENVKQVLSAGVDGIAVVSAVMGASDVKKA 194
+ +R I ++P AIGGI++ NV+ VL AG IAVV AV+ A D A
Sbjct: 209 PGRVAAGLGYVRHIAALRPTLPWYAIGGIDLGNVQAVLDAGATRIAVVRAVLDADDCAAA 268
Query: 195 TEELRKII 202
LR+ +
Sbjct: 269 AHALREAL 276
>gb|AAF25544.1|AF109218_4 (AF109218) ThiE [Staphylococcus carnosus]
Length = 212
Score = 104 bits (258), Expect = 6e-22
Identities = 68/208 (32%), Positives = 114/208 (54%), Gaps = 16/208 (7%)
Query: 7 LKLYVITDRRLKPEVKSV----RQALEGGATSIQLRIKDASTK---EMYEVGKEIRRLTQ 59
L +Y I + PE +++ ++ALEGG T Q R K K + + KE++ L +
Sbjct: 7 LNVYFICGTQDIPEGRTIQEVLKEALEGGITLYQFREKGNGAKTGQDKVALAKELQALCK 66
Query: 60 EYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKK 119
Y+ F V+D + +A ++ADG+ +G +D ++ IIG S+ +LEE ++
Sbjct: 67 SYNVPFIVNDDVALAEEIDADGIHVGQDDEAVDDFNNRFEGKIIGLSIGNLEELNASDLT 126
Query: 120 GADYLGAGSVFPTKTKRD----VRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAG 175
DY+G G +F T +K D V IE LR+ E +P+VAIGGI+++NV++V
Sbjct: 127 YVDYIGVGPIFATPSKDDASEPVGPKMIETLRK--EVGDLPIVAIGGISLDNVQEVAKTS 184
Query: 176 VDGIAVVSAVMGASDVKKATEELRKIIE 203
DG++V+SA+ + V TE + K ++
Sbjct: 185 ADGVSVISAIARSPHV---TETVHKFLQ 209
>sp|O25514|THIE_HELPY PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE) >gi|7427959|pir||C64625 probable
thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) -
Helicobacter pylori (strain 26695)
>gi|2313974|gb|AAD07889.1| (AE000595) thiamin phosphate
pyrophosphorylase/hyroxyethylthiazole kinase (thiB)
[Helicobacter pylori 26695]
Length = 219
Score = 103 bits (255), Expect = 1e-21
Identities = 68/190 (35%), Positives = 109/190 (56%), Gaps = 9/190 (4%)
Query: 16 RLKPEVKSVRQALEGGATSIQLRIKD----ASTKEMYEVGKEIRRLTQEYDALFFVDDRI 71
R+ + ++ AL+ T+ Q R K ++ ++ + ++L Q+Y A F V+D +
Sbjct: 28 RINALLDTLELALQSKITAFQFRQKGDLALQDPTQIKQLAMKCQKLCQKYGAPFIVNDEV 87
Query: 72 DVALAVNADGVQLGPEDMPI-EVAREIAPNLIIGASVYSLEEALEAEKKGA-DYLGAGSV 129
+AL + ADGV +G EDM I EV IG SV +LE+AL+A A YLG G +
Sbjct: 88 QLALELKADGVHVGQEDMAIEEVITLCKKRQFIGLSVNTLEQALKARHLDAVAYLGVGPI 147
Query: 130 FPTKTKRDVRVIRIEGLREIVEA-VSIPVVAIGGINVENVKQVLSAGVDGIAVVSAVMGA 188
FPT +K+D +V+ +E L++I ++ + P++AIGGI + N ++ G GIAV+SA+ A
Sbjct: 148 FPTPSKKDKQVVGVELLKKIKDSGIKKPLIAIGGITMHNAPKLREYG--GIAVISAIAQA 205
Query: 189 SDVKKATEEL 198
D A +L
Sbjct: 206 KDKALAVGKL 215
>sp|Q9ZL01|THIE_HELPJ PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE) >gi|7434088|pir||G71889 thiamin-phosphate
pyrophosphorylase (EC 2.5.1.3) - Helicobacter pylori
(strain J99) >gi|4155348|gb|AAD06361.1| (AE001508)
THIAMINE PHOSPHATE PYROPHOSPHORYLASE [Helicobacter
pylori J99]
Length = 217
Score = 100 bits (247), Expect = 1e-20
Identities = 71/199 (35%), Positives = 112/199 (55%), Gaps = 10/199 (5%)
Query: 10 YVITDRRLKPEVKSVRQALEGGATSIQLRIK-DASTKEMYEVGK---EIRRLTQEYDALF 65
Y I R + ++ AL+ T+ Q R K D + ++ E+ + E ++L ++Y F
Sbjct: 19 YHIKGDRTNALLDTLELALQSKITAFQFRQKGDLALQDPVEIKRLALECQKLCKKYGTPF 78
Query: 66 FVDDRIDVALAVNADGVQLGPEDMPI-EVAREIAPNLIIGASVYSLEEALEAEK-KGADY 123
++D + +AL + ADGV +G EDM I EV L IG SV +LE+AL+A Y
Sbjct: 79 IINDEVRLALELKADGVHVGQEDMAIEEVVTLCQKRLFIGLSVNTLEQALKARHLDHIAY 138
Query: 124 LGAGSVFPTKTKRDVR-VIRIEGLREIVEA-VSIPVVAIGGINVENVKQVLSAGVDGIAV 181
LG G +FPT +K+D + V+ + L++I ++ V P++AIGGI +N ++ GIAV
Sbjct: 139 LGVGPIFPTPSKKDAKEVVGVNLLKKIHDSGVEKPLIAIGGITTDNASKLQK--FSGIAV 196
Query: 182 VSAVMGASDVKKATEELRK 200
+SA+ A D A E+L K
Sbjct: 197 ISAITQAKDKALAVEKLLK 215
>sp|P96260|THIE_MYCTU PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE) >gi|7434089|pir||D70629 probable thiE protein
- Mycobacterium tuberculosis (strain H37RV)
>gi|1817680|emb|CAB06584.1| (Z84724) thiE [Mycobacterium
tuberculosis]
Length = 222
Score = 98.7 bits (242), Expect = 4e-20
Identities = 72/209 (34%), Positives = 103/209 (48%), Gaps = 14/209 (6%)
Query: 8 KLYVITD-RRLKPEVKSVRQA-LEGGATSIQLRIKDA---------STKEMYEVGKEIRR 56
+LY+ TD RR + ++ +A L GG IQLR K + ++ + +
Sbjct: 10 RLYLCTDARRERGDLAQFAEAALAGGVDIIQLRDKGSPGELRFGPLQARDELAACEILAD 69
Query: 57 LTQEYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALE 115
Y ALF V+DR D+A A AD + LG D+P+ VAR+I AP+ +IG S + ++
Sbjct: 70 AAHRYGALFAVNDRADIARAAGADVLHLGQRDLPVNVARQILAPDTLIGRSTHDPDQVAA 129
Query: 116 AEKKGADYLGAGSVFPTKTKRDVRVIRIEGLREIVE--AVSIPVVAIGGINVENVKQVLS 173
A ADY G +PT TK + +R E P AIGGIN + + VL
Sbjct: 130 AAAGDADYFCVGPCWPTPTKPGRAAPGLGLVRVAAELGGDDKPWFAIGGINAQRLPAVLD 189
Query: 174 AGVDGIAVVSAVMGASDVKKATEELRKII 202
AG I VV A+ A D + A E+LR +
Sbjct: 190 AGARRIVVVRAITSADDPRAAAEQLRSAL 218
>gb|AAF33508.1| (AF170176) 90% identity over 211 amino acids with E. coli thiamin
phosphate pyrophosphorylase (THIE) (SW:P30137)
[Salmonella typhimurium LT2]
Length = 211
Score = 94.4 bits (231), Expect = 9e-19
Identities = 71/201 (35%), Positives = 103/201 (50%), Gaps = 12/201 (5%)
Query: 6 KLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALF 65
+L LY + D V+ + + LE G +IQLRIKD +E+ L + Y+A
Sbjct: 12 RLGLYPVVD-----SVEWIERLLESGVRTIQLRIKDKRDEEVEADVIAAIALGRRYNARL 66
Query: 66 FVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALEAEKKGADYL 124
F++D +A+ A GV LG ED+ + I A L +G S + E A Y+
Sbjct: 67 FINDYWRLAIKHRAYGVHLGQEDLETTDLKAIQAAGLRLGVSTHDDMEIDVALAAKPSYI 126
Query: 125 GAGSVFPTKTKRDVRVIRIEGLREIVEAVS----IPVVAIGGINVENVKQVLSAGVDGIA 180
G VFPT+TK+ +GL ++ + P VAIGGI++E VL+ GV IA
Sbjct: 127 ALGHVFPTQTKQMPSAP--QGLAQLASHIERLADYPTVAIGGISLERAPAVLATGVGSIA 184
Query: 181 VVSAVMGASDVKKATEELRKI 201
VVSA+ A+D ++AT EL I
Sbjct: 185 VVSAITQAADWREATAELLAI 205
>sp|P25053|TENI_BACSU REGULATORY PROTEIN TENI >gi|73041|pir||XMBSTI regulatory protein
tenI - Bacillus subtilis >gi|143730|gb|AAA22849.1|
(M73546) transcription activator [Bacillus subtilis]
>gi|2633520|emb|CAB13023.1| (Z99110) transcriptional
regulator [Bacillus subtilis]
Length = 205
Score = 93.6 bits (229), Expect = 1e-18
Identities = 65/208 (31%), Positives = 111/208 (53%), Gaps = 18/208 (8%)
Query: 7 LKLYVITDRRLKP--EVKSVRQALEGGATSIQLRIKDASTKEM-------YEVGKEIRRL 57
++L+ ITD KP E+ + ++ I +R + S ++ +E G + R+L
Sbjct: 1 MELHAITDDS-KPVEELARIIITIQNEVDFIHIRERSKSAADILKLLDLIFEGGIDKRKL 59
Query: 58 TQEYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAE 117
++ R+D+AL VQL + R P+L IG SV+SLEEA++AE
Sbjct: 60 V--------MNGRVDIALFSTIHRVQLPSGSFSPKQIRARFPHLHIGRSVHSLEEAVQAE 111
Query: 118 KKGADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVD 177
K+ ADY+ G VF T K+ + + L +I + +SIPV+AIGG+ + ++ V AG D
Sbjct: 112 KEDADYVLFGHVFETDCKKGLEGRGVSLLSDIKQRISIPVIAIGGMTPDRLRDVKQAGAD 171
Query: 178 GIAVVSAVMGASDVKKATEELRKIIEEV 205
GIAV+S + +++ +A + ++E+
Sbjct: 172 GIAVMSGIFSSAEPLEAARRYSRKLKEM 199
>gb|AAD56925.1|AF180145_17 (AF180145) thiamine-phosphate pyrophosphorylase [Zymomonas mobilis]
Length = 238
Score = 92.1 bits (225), Expect = 4e-18
Identities = 65/204 (31%), Positives = 110/204 (53%), Gaps = 7/204 (3%)
Query: 9 LYVITDRRLKPE-VKSVRQALEGGATS-IQLRIKDASTKEMYEVGKEIRRLTQEYDALFF 66
LY+I+ + V+ +++A +GG S QLR+K + + + + ++++ + D F
Sbjct: 31 LYLISPPEIDEHFVERLKKAFDGGDVSAFQLRLKGLNEHAIARLAEPLQKVCADRDVAFI 90
Query: 67 VDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEE-ALEAEKKGADYL 124
V+D + +A + ADGV LG D AR I P+ IG + ++ A+ A +KGADY+
Sbjct: 91 VNDSVSLAKRLGADGVHLGQGDGDAAEARVILGPSAQIGVTCHNSRHLAMIAGEKGADYV 150
Query: 125 GAGSVFPTKTKRDVRVI-RIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVS 183
G+ +PT T +DVR R E L +P VAIGGI ENV ++ AG D +AV +
Sbjct: 151 AFGAFYPT-TSKDVRYYARPEILSWWATLFELPSVAIGGITTENVAPIVKAGADFVAVCA 209
Query: 184 AVMGASDVK-KATEELRKIIEEVL 206
+ A + + KA ++++ +
Sbjct: 210 GIWKAKEGEDKAVAHFNTVLDQAV 233
>sp|Q9ZBL5|THIE_MYCLE PROBABLE THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP
PYROPHOSPHORYLASE) (TMP-PPASE) (THIAMINE-PHOSPHATE
SYNTHASE) >gi|11258836|pir||T44738 probable
thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)
[imported] - Mycobacterium leprae
>gi|4154053|emb|CAA22707.1| (AL035159) probable
thiamine-phosphate pyrophosphorylase [Mycobacterium
leprae]
Length = 235
Score = 90.5 bits (221), Expect = 1e-17
Identities = 74/210 (35%), Positives = 105/210 (49%), Gaps = 17/210 (8%)
Query: 6 KLKLYVITDRRLKPE--VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRL------ 57
K +LY+ TD R + + V AL GG +QLR K + ++ + + L
Sbjct: 18 KARLYLCTDARRERGDLAQFVNAALAGGVDIVQLRDKGSVGEQQFGPLEARDALAACEIF 77
Query: 58 ---TQEYDALFFVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEE- 112
T +DALF V+DR D+A A AD + LG D+P VAR+I ++IG S + +
Sbjct: 78 TDATGRHDALFAVNDRADIARAAGADVLHLGQGDLPPGVARQIVCRQMLIGLSTHDRHQV 137
Query: 113 --ALEAEKKG-ADYLGAGSVFPTKTKRDVRVIRIEGLREIVE-AVSIPVVAIGGINVENV 168
A+ A G DY G +PT TK D +E +R E A P AIGGI+ + +
Sbjct: 138 AAAVAALDAGLVDYFCVGPCWPTPTKPDRPAPGLELVRAAAELAGDKPWFAIGGIDAQRL 197
Query: 169 KQVLSAGVDGIAVVSAVMGASDVKKATEEL 198
VL AG I VV A+ A+D + A E+L
Sbjct: 198 PDVLHAGARRIVVVRAITAAADPRAAAEQL 227
>sp|P30137|THIE_ECOLI THIAMINE-PHOSPHATE PYROPHOSPHORYLASE (TMP PYROPHOSPHORYLASE)
(TMP-PPASE) (THIAMINE-PHOSPHATE SYNTHASE)
>gi|421173|pir||S35118 thiamin-phosphate
pyrophosphorylase (EC 2.5.1.3) thiE - Escherichia coli
>gi|396332|gb|AAC43091.1| (U00006) thiE [Escherichia
coli] >gi|414233|gb|AAB95617.1| (M88701) thiE
[Escherichia coli] >gi|1790426|gb|AAC76967.1| (AE000473)
thiamin biosynthesis, thiazole moiety [Escherichia coli
K12]
Length = 211
Score = 87.4 bits (213), Expect = 1e-16
Identities = 67/196 (34%), Positives = 101/196 (51%), Gaps = 8/196 (4%)
Query: 9 LYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALFFVD 68
LY + D V+ + + L+ G ++QLRIKD +E+ L + Y+A F++
Sbjct: 15 LYPVVD-----SVQWIERLLDAGVRTLQLRIKDRRDEEVEADVVAAIALGRRYNARLFIN 69
Query: 69 DRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALEAEKKGADYLGAG 127
D +A+ A GV LG ED+ I A L +G S + E A Y+ G
Sbjct: 70 DYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAGLRLGVSTHDDMEIDVALAARPSYIALG 129
Query: 128 SVFPTKTKRDVRVIR-IEGLREIVEAVS-IPVVAIGGINVENVKQVLSAGVDGIAVVSAV 185
VFPT+TK+ + +E L VE ++ P VAIGGI++ V++ GV IAVVSA+
Sbjct: 130 HVFPTQTKQMPSAPQGLEQLARHVERLADYPTVAIGGISLARAPAVIATGVGSIAVVSAI 189
Query: 186 MGASDVKKATEELRKI 201
A+D + AT +L +I
Sbjct: 190 TQAADWRLATAQLLEI 205
>pir||A82814 thiamin-phosphate pyrophosphorylase XF0378 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9105210|gb|AAF83188.1|AE003889_5 (AE003889)
thiamin-phosphate pyrophosphorylase [Xylella fastidiosa]
Length = 234
Score = 87.4 bits (213), Expect = 1e-16
Identities = 59/185 (31%), Positives = 97/185 (51%), Gaps = 4/185 (2%)
Query: 9 LYVITDRRLKPEVKSVRQA-LEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALFFV 67
+Y+IT R A L G +Q R K A+T E + + L ++ +
Sbjct: 37 IYLITPDETDTARLIARTAPLLNGIVWLQYRNKLANTALRTEQAQALLALCRQTGIPLLI 96
Query: 68 DDRIDVALAVNADGVQLGPEDMPIEVAR-EIAPNLIIGASVYS-LEEALEAEKKGADYLG 125
+D +++A + ADGV LG D +AR ++ P+ IIG S Y+ +E A +A K GA Y+G
Sbjct: 97 NDDLELAQTIGADGVHLGMHDSNPSIARAQLGPHAIIGVSCYNQIERAKQAIKAGASYVG 156
Query: 126 AGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVSAV 185
G+ +P+ TK E LR+ + +P VAIGG+ +N+ ++ AG + +AV+S +
Sbjct: 157 FGTFYPSHTKTTPYRATPELLRQTTH-LGVPRVAIGGLTPKNIAPIIEAGAELLAVISGI 215
Query: 186 MGASD 190
A +
Sbjct: 216 YSAKN 220
>pir||A82369 thiamin-phosphate pyrophosphorylase VC0062 [imported] - Vibrio
cholerae (group O1 strain N16961)
>gi|9654457|gb|AAF93240.1| (AE004097) thiamin-phosphate
pyrophosphorylase [Vibrio cholerae]
Length = 440
Score = 86.2 bits (210), Expect = 2e-16
Identities = 61/206 (29%), Positives = 98/206 (46%), Gaps = 16/206 (7%)
Query: 4 REKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDA 63
+ K LY + D +V + L+ G ++QLRIKD ++ L +E++A
Sbjct: 204 KAKFNLYPVVD-----DVNWIEHLLKLGVRTVQLRIKDPKQGDLEAQIIRAIALGREFNA 258
Query: 64 LFFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAP-NLIIGASVYSLEEALEAEKKGAD 122
F++D +A+ A GV LG ED+ E+ + +G S + E L A
Sbjct: 259 QVFINDHWQLAIKHQAYGVHLGQEDLTSANLTELLDAGIRLGLSTHGYYELLIAAGIQPS 318
Query: 123 YLGAGSVFPTKTK----RDVRVIRIEGLREIVEAV------SIPVVAIGGINVENVKQVL 172
Y+ G +FPT TK + ++R+ + +V + IP VAIGGI+ N++ VL
Sbjct: 319 YIALGHIFPTTTKQMPSKPQGLVRLAAYQRLVNQMPYQGQHGIPTVAIGGIDCRNIRDVL 378
Query: 173 SAGVDGIAVVSAVMGASDVKKATEEL 198
GV +AVV A+ + D A + L
Sbjct: 379 DCGVTAVAVVRAITESPDPSLAVQAL 404
>pir||T34974 probable thiamin phosphate pyrophosphorylase - Streptomyces
coelicolor >gi|5689976|emb|CAB52013.1| (AL109663)
putative thiamin phosphate pyrophosphorylase
[Streptomyces coelicolor A3(2)]
Length = 223
Score = 85.8 bits (209), Expect = 3e-16
Identities = 66/202 (32%), Positives = 97/202 (47%), Gaps = 5/202 (2%)
Query: 8 KLYVITD-RRLKPEVKSVRQA-LEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALF 65
+LY+ TD RR + ++ A L GG +QLR K E E + L
Sbjct: 17 RLYLCTDARRSQGDLPEFLDAVLAGGVDIVQLRDKGMEAAEELEHLAVFADACARHGKLL 76
Query: 66 FVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYSLEEALEAE-KKGADY 123
V+DR DVA A AD + LG D+P+ AR I +++IG S ++ EA A ++G DY
Sbjct: 77 AVNDRADVAHAARADVLHLGQGDLPVPAARAILGDDVLIGRSTHAESEAAAAAAQEGVDY 136
Query: 124 LGAGSVFPTKTKRDVRVIRIEGLREIVE-AVSIPVVAIGGINVENVKQVLSAGVDGIAVV 182
G +PT TK ++ +R P AIGGI++ N+ +VL AG + VV
Sbjct: 137 FCTGPCWPTPTKPGRHAPGLDLVRRTAALGTDRPWFAIGGIDLGNLDEVLEAGARRVVVV 196
Query: 183 SAVMGASDVKKATEELRKIIEE 204
A+ A D A E + + +
Sbjct: 197 RAITAAEDPGAAAAEFARRLRQ 218
>pir||E82032 thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) NMA0363 [imported]
- Neisseria meningitidis (group A strain Z2491)
>gi|7379115|emb|CAB83664.1| (AL162752) thiamin-phosphate
pyrophosphorylase [Neisseria meningitidis Z2491]
Length = 205
Score = 85.4 bits (208), Expect = 4e-16
Identities = 63/190 (33%), Positives = 94/190 (49%), Gaps = 8/190 (4%)
Query: 4 REKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDA 63
+ LK Y + P V + +E GA ++QLR K E+ Q
Sbjct: 7 KSPLKFYAVV-----PTADWVERMVEAGADTVQLRCKALHGDELKREIARCVAACQGSHT 61
Query: 64 LFFVDDRIDVALAVNADGVQLGPEDMPI-EVAREIAPNLIIGASVYSLEEALEAEKKGAD 122
F++D A+ A GV LG EDM ++A A L +G S +S+ E A
Sbjct: 62 QLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRLGLSTHSVAELDRALSVHPS 121
Query: 123 YLGAGSVFPTKTKRDVRVIR-IEGLREIV-EAVSIPVVAIGGINVENVKQVLSAGVDGIA 180
Y+ +G++FPT TK+ + ++ LRE V +A PVVAIGGI++ N + VL+ GV +A
Sbjct: 122 YIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVAIGGIDLNNARAVLATGVSSLA 181
Query: 181 VVSAVMGASD 190
V AV A++
Sbjct: 182 AVRAVTKAAN 191
>pir||F83149 thiamin-phosphate pyrophosphorylase PA3976 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9950166|gb|AAG07363.1|AE004815_7 (AE004815)
thiamin-phosphate pyrophosphorylase [Pseudomonas
aeruginosa]
Length = 209
Score = 84.3 bits (205), Expect = 1e-15
Identities = 58/196 (29%), Positives = 97/196 (48%), Gaps = 6/196 (3%)
Query: 9 LYVITDRRLKPE---VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALF 65
LY ITD +L + + V AL GGA +Q R K + + +R L + + A
Sbjct: 6 LYAITDSQLLDDGRLLPYVEAALRGGARLLQYRDKSSDQARRLREAESLRELCERHGAQL 65
Query: 66 FVDDRIDVALAVNADGVQLGPEDMPIEVAREI-APNLIIGASVYS-LEEALEAEKKGADY 123
V+D ++A + G+ LG D + AR + IIGA+ ++ LE A +A +GA Y
Sbjct: 66 IVNDDAELAARLGV-GLHLGQTDGSLSAARALLGRQAIIGATCHAQLELAEQAVAEGASY 124
Query: 124 LGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVVS 183
+ G F + TK +E L + + +P+ AIGGI+++ +++ GVD +AV+
Sbjct: 125 VAFGRFFNSSTKPGAPAASVELLDQARPRLHLPITAIGGISLDTAPGLIARGVDLVAVIH 184
Query: 184 AVMGASDVKKATEELR 199
A+ A+ + R
Sbjct: 185 ALFAAASAAEVERRAR 200
>pir||A81011 thiamin-phosphate pyrophosphorylase NMB2069 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7227331|gb|AAF42388.1| (AE002556) thiamin-phosphate
pyrophosphorylase [Neisseria meningitidis MC58]
Length = 205
Score = 83.5 bits (203), Expect = 2e-15
Identities = 62/190 (32%), Positives = 94/190 (48%), Gaps = 8/190 (4%)
Query: 4 REKLKLYVITDRRLKPEVKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDA 63
+ LK Y + P V + ++ GA ++QLR K E+ Q
Sbjct: 7 KSPLKFYAVV-----PTADWVGRMVKAGADTVQLRCKALHGDELKREIARCAAACQGSRT 61
Query: 64 LFFVDDRIDVALAVNADGVQLGPEDMPI-EVAREIAPNLIIGASVYSLEEALEAEKKGAD 122
F++D A+ A GV LG EDM ++A A L +G S +S+ E A
Sbjct: 62 QLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRLGLSTHSVAELDRALSVHPS 121
Query: 123 YLGAGSVFPTKTKRDVRVIR-IEGLREIV-EAVSIPVVAIGGINVENVKQVLSAGVDGIA 180
Y+ +G++FPT TK+ + ++ LRE V +A PVVAIGGI++ N + VL+ GV +A
Sbjct: 122 YIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVAIGGIDLNNARAVLATGVSSLA 181
Query: 181 VVSAVMGASD 190
V AV A++
Sbjct: 182 AVRAVTEAAN 191
>pir||H81306 probable transferase Cj1043c [imported] - Campylobacter jejuni
(strain NCTC 11168) >gi|6968479|emb|CAB73299.1|
(AL139077) possible transferase [Campylobacter jejuni]
Length = 201
Score = 71.4 bits (172), Expect = 7e-12
Identities = 56/202 (27%), Positives = 96/202 (46%), Gaps = 17/202 (8%)
Query: 8 KLYVITDRRLKPE--VKSVRQALEGGATSIQLRIKDASTKEMYEVGKEIRRLTQEYDALF 65
K+ I+DR+ +K + + + +I LR KD S E Y++ KE+ + +
Sbjct: 5 KIIAISDRKCVEIDFLKQIEKLAKAKVDAIVLREKDLSEFEYYDLAKEVLSICAKQKVTC 64
Query: 66 FVDDRIDVALAVNADGVQLGPE--DMPIEVAREIAPNL-----IIGASVYSLEEALEAEK 118
F+ + + ++LG P+ + R+ P L I+G SV+S EE LEA
Sbjct: 65 FLH-------FFDRECLKLGHRYFHAPLSLLRK-EPKLTKYFHILGTSVHSKEELLEAMS 116
Query: 119 KGADYLGAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDG 178
+Y G +F + K + I+ L+ ++E IP+ AIGGIN +N++ V G
Sbjct: 117 YKVNYAFVGHIFESSCKMGLEPKGIDFLKSLLEFSQIPLYAIGGINAQNIENFKDINVVG 176
Query: 179 IAVVSAVMGASDVKKATEELRK 200
+ + +M D+KK E R+
Sbjct: 177 VCMREILMKEKDLKKYLLECRQ 198
>pir||E82720 bifunctional DGTP-pyrophosphohydrolase/thiamin phosphate synthase
XF1120 [imported] - Xylella fastidiosa (strain 9a5c)
>gi|9106080|gb|AAF83930.1|AE003948_2 (AE003948)
bifunctional DGTP-pyrophosphohydrolase/thiamine
phosphate synthase [Xylella fastidiosa]
Length = 320
Score = 70.2 bits (169), Expect = 2e-11
Identities = 50/178 (28%), Positives = 85/178 (47%), Gaps = 8/178 (4%)
Query: 10 YVITDRRLKPEVK---SVRQALEGGATSIQLRIKDASTKEMYEVGKEI--RRLTQEYDAL 64
Y++T K + + +V +A G IQLR++ + +E RR + L
Sbjct: 136 YLVTPEPGKQDAEWLDAVERAYRLGIERIQLRMRQHDPARWSGLVREAVQRRGRAHVEVL 195
Query: 65 FFVDDRIDVALAVNADGVQLGPEDMPIEVAREIAPNLIIGASVYSLEEALEAEKKGADYL 124
D + A+ + GV LG E + + AR + L +GAS + L + A++ G D+
Sbjct: 196 LNRDIALAEAVGI---GVHLGAEQLAVLDARPLPVGLPVGASCHCLADLRHAQRIGCDFA 252
Query: 125 GAGSVFPTKTKRDVRVIRIEGLREIVEAVSIPVVAIGGINVENVKQVLSAGVDGIAVV 182
G V PT++ + EG ++ E V++P+ AIGG+ + VK+ G GIA +
Sbjct: 253 VLGPVLPTESHPGAVTLGWEGFEQLREQVALPIYAIGGMGADQVKEARRHGAQGIAAM 310
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.316 0.136 0.357
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65270983
Number of Sequences: 2977
Number of extensions: 2471723
Number of successful extensions: 9408
Number of sequences better than 1.0e-10: 35
Number of HSP's better than 0.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 9329
Number of HSP's gapped (non-prelim): 36
length of query: 207
length of database: 189,106,746
effective HSP length: 61
effective length of query: 146
effective length of database: 152,599,039
effective search space: 22279459694
effective search space used: 22279459694
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 163 (67.9 bits)