BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1646 (thiD) DE:hydroxymethylpyrimidine phosphate kinase
(thiD)
(446 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75087 hydroxymethylpyrimidine phosphate kinase (thid) PAB1... 886 0.0
pir||E71057 probable thiamin biosynthesis protein - Pyrococcus h... 814 0.0
pir||G72469 probable phosphomethylpyrimidine kinase APE2400 - Ae... 467 e-130
gb|AAK42540.1| Phosphomethylpyrimidine kinase (thiD-2) [Sulfolob... 243 3e-63
gb|AAG20645.1| (AE005133) hydroxymethylpyrimidine phosphate kina... 237 2e-61
pir||D81807 phosphomethylpyrimidine kinase (EC 2.7.4.7) NMA1815 ... 224 2e-57
pir||F81061 phosphomethylpyrimidine kinase NMB1616 [imported] - ... 223 4e-57
sp|P44697|THID_HAEIN PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 222 8e-57
pir||F69845 phosphomethylpyrimidine kinase homolog yjbV - Bacill... 217 2e-55
emb|CAC12405.1| (AL445067) probable phosphomethylpyrimidine kina... 217 3e-55
dbj|BAB05154.1| (AP001512) phosphomethylpyrimidine kinase [Bacil... 216 3e-55
sp|P56904|THID_RHIME PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 215 6e-55
gb|AAF76451.1|AF077841_2 (AF077841) hydroxymethylpyrimidine-phos... 215 8e-55
sp|Q57688|Y236_METJA HYPOTHETICAL PROTEIN MJ0236 >gi|2128191|pir... 213 4e-54
gb|AAF25542.1|AF109218_2 (AF109218) ThiD [Staphylococcus carnosus] 206 5e-52
sp|O67772|THID_AQUAE PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 205 7e-52
sp|Q9ZBR6|THID_STRCO PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 205 1e-51
pir||D82784 phosphomethylpyrimidine kinase XF0621 [imported] - X... 204 2e-51
sp|P96268|THID_MYCTU PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 201 1e-50
sp|O85786|THID_SYNP7 PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 198 1e-49
sp|Q9ZBL1|THID_MYCLE PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 197 3e-49
gb|AAK40369.1| Phosphomethylpyrimidine kinase (thiD-1) [Sulfolob... 194 1e-48
sp|Q9ZL00|THID_HELPJ PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 193 3e-48
sp|O25515|THID_HELPY PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 192 5e-48
sp|P39610|THID_BACSU PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 191 1e-47
pir||F69181 transcription regulator - Methanobacterium thermoaut... 191 1e-47
pir||G72333 conserved hypothetical protein - Thermotoga maritima... 188 1e-46
pir||D82217 phosphomethylpyrimidine kinase VC1296 [imported] - V... 186 5e-46
pir||E81311 phosphomethylpyrimidine kinase (EC 2.7.4.7) Cj1082c ... 184 2e-45
gb|AAB72162.1| (AF000657) probable thiamin biosynthetic enzyme [... 183 2e-45
pir||T07834 hydroxymethylpyrimidine kinase (EC 2.7.1.49) / thiam... 182 1e-44
pir||F75613 phosphomethylpyrimidine kinase - Deinococcus radiodu... 181 1e-44
sp|P76422|THID_ECOLI PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 180 3e-44
pir||T47257 thiamin biosynthesis protein thi-4 [imported] - Neur... 178 1e-43
sp|P55882|THID_SALTY PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 171 1e-41
sp|O83153|THID_TREPA PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHA... 167 3e-40
pir||T40812 probable thiamin biosynthesis protein - fission yeas... 163 3e-39
gi|6324997 Thi21p [Saccharomyces cerevisiae] >gi|2132251|pir||S6... 154 2e-36
emb|CAA62531.1| (X91067) transcription factor [Saccharomyces cer... 147 2e-34
gi|6324517 Thi20p [Saccharomyces cerevisiae] >gi|2133057|pir||S6... 147 2e-34
pir||T40811 probable thiamin biosynthesis protein - fission yeas... 144 2e-33
gi|6325378 Thi22p [Saccharomyces cerevisiae] >gi|2132289|pir||S6... 141 1e-32
gi|11499790 hydroxymethylpyrimidine phosphate kinase (thiD) [Arc... 136 7e-31
pir||A69215 conserved hypothetical protein MTH861 - Methanobacte... 129 8e-29
gb|AAC27706.1| (AF061946) putative kinase Pak1p [Pichia angusta] 123 4e-27
pir||E83149 phosphomethylpyrimidine kinase PA3975 [imported] - P... 123 5e-27
pir||S14885 hypothetical protein 8 - yeast (Pichia angusta) >gi|... 120 3e-26
pir||S39961 Thi protein - Rhizobium meliloti >gi|742332|prf||200... 118 2e-25
gb|AAD39740.1| (AF105331) phosphomethylpyrimidine kinase [Clostr... 117 3e-25
pir||T41198 probable thiamin biosynthesis protein - fission yeas... 111 2e-23
gb|AAK40404.1| Conserved hypothetical protein [Sulfolobus solfat... 106 4e-22
pir||E71118 hypothetical protein PH0716 - Pyrococcus horikoshii ... 94 2e-18
pir||H75042 hypothetical protein PAB0877 - Pyrococcus abyssi (st... 87 4e-16
sp|P40191|PDXK_ECOLI PYRIDOXINE KINASE (PYRIDOXAL KINASE) (VITAM... 74 4e-12
gb|AAK40790.1| Conserved hypothetical protein [Sulfolobus solfat... 71 2e-11
>pir||C75087 hydroxymethylpyrimidine phosphate kinase (thid) PAB1646 -
Pyrococcus abyssi (strain Orsay)
>gi|5458512|emb|CAB50000.1| (AJ248286)
hydroxymethylpyrimidine phosphate kinase (thiD)
[Pyrococcus abyssi]
Length = 446
Score = 886 bits (2264), Expect = 0.0
Identities = 446/446 (100%), Positives = 446/446 (100%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV
Sbjct: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRED 120
VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRED
Sbjct: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRED 120
Query: 121 AVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI 180
AVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI
Sbjct: 121 AVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI 180
Query: 181 EGEEAIDVLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT 240
EGEEAIDVLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT
Sbjct: 181 EGEEAIDVLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT 240
Query: 241 LGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPVKFIPEVGTNFVY 300
LGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPVKFIPEVGTNFVY
Sbjct: 241 LGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPVKFIPEVGTNFVY 300
Query: 301 SLPLPYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAIN 360
SLPLPYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAIN
Sbjct: 301 SLPLPYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAIN 360
Query: 361 IRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLG 420
IRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLG
Sbjct: 361 IRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLG 420
Query: 421 DVGKEPMILIFGRNPREVLDKLRLLL 446
DVGKEPMILIFGRNPREVLDKLRLLL
Sbjct: 421 DVGKEPMILIFGRNPREVLDKLRLLL 446
>pir||E71057 probable thiamin biosynthesis protein - Pyrococcus horikoshii
>gi|3257572|dbj|BAA30255.1| (AP000005) 446aa long
hypothetical thiamine biosynthesis protein [Pyrococcus
horikoshii]
Length = 446
Score = 814 bits (2079), Expect = 0.0
Identities = 396/446 (88%), Positives = 427/446 (94%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
MRLV+TALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQ VTAIHDIPP+V
Sbjct: 1 MRLVRTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQTVTAIHDIPPEV 60
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRED 120
VSEQIR VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRED
Sbjct: 61 VSEQIRVVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRED 120
Query: 121 AVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI 180
AVDALIKYILPLATLVTPNRFEAEKL+GM+IR V DAK+AAKKIAEETGAKAVIVKGGH+
Sbjct: 121 AVDALIKYILPLATLVTPNRFEAEKLTGMEIRNVNDAKLAAKKIAEETGAKAVIVKGGHL 180
Query: 181 EGEEAIDVLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT 240
EG+EAIDVLYHNG+Y+LYRAPKV+GCTHGTGCSFSAAITANLAKG LEDA+ VAK+FIT
Sbjct: 181 EGKEAIDVLYHNGAYKLYRAPKVDGCTHGTGCSFSAAITANLAKGLSLEDAVSVAKEFIT 240
Query: 241 LGIAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPVKFIPEVGTNFVY 300
LGI+ GH+IGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFE +NP+K IPEVGTNFVY
Sbjct: 241 LGISTGHKIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFERINPIKLIPEVGTNFVY 300
Query: 301 SLPLPYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAIN 360
+LP PYART++DVAGVKGRIVRVGNEVRAVG VEFGASDHLARA+LTFM+FYP+YRSAIN
Sbjct: 301 ALPFPYARTKEDVAGVKGRIVRVGNEVRAVGSVEFGASDHLARAILTFMRFYPKYRSAIN 360
Query: 361 IRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLG 420
+RYSEE V LA+EQG VSFYDRREEP E+KE+EGATIPWGIE A+KR+ +RPDVIYHLG
Sbjct: 361 VRYSEEIVELAKEQGFTVSFYDRREEPIEVKEKEGATIPWGIEVAVKRINKRPDVIYHLG 420
Query: 421 DVGKEPMILIFGRNPREVLDKLRLLL 446
D+GKEPMILIFGRNPREVLDKLRLL+
Sbjct: 421 DIGKEPMILIFGRNPREVLDKLRLLI 446
>pir||G72469 probable phosphomethylpyrimidine kinase APE2400 - Aeropyrum pernix
(strain K1) >gi|5106104|dbj|BAA81415.1| (AP000064) 464aa
long hypothetical phosphomethylpyrimidine kinase
[Aeropyrum pernix]
Length = 464
Score = 467 bits (1189), Expect = e-130
Identities = 245/439 (55%), Positives = 308/439 (69%), Gaps = 5/439 (1%)
Query: 4 VKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSE 63
+ ALTIAGSDSGGGAGI+ADLKTF GVHG AITS+TAQNT V AIHD+PPDVV
Sbjct: 13 IPVALTIAGSDSGGGAGIQADLKTFAVMGVHGASAITSITAQNTMEVRAIHDVPPDVVKA 72
Query: 64 QIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
Q+ AVA+D+GVDAAKTGMLSN IIKAVA+ V Y FPLVVDPVM+AKSGA LL+ +A +
Sbjct: 73 QVEAVADDLGVDAAKTGMLSNTGIIKAVAEVVDEYGFPLVVDPVMVAKSGARLLKPEAEE 132
Query: 124 ALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGE 183
AL K ++P AT+VTPN EAE L+G+K+ ++ A+ AA+ I EE GA+A +VKGGH+ GE
Sbjct: 133 ALKKLVIPRATIVTPNAPEAEVLTGVKVVDLDTAREAARIIVEELGAEAAVVKGGHLRGE 192
Query: 184 EAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLG 242
+DVLYH G YRL++ ++E THGTGC+FSAAI A LAKG +E+A++VAKKFIT+
Sbjct: 193 YVVDVLYHRGEYRLFKERRIEKATTHGTGCAFSAAIAAGLAKGRSVEEAVDVAKKFITVA 252
Query: 243 IAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPV--KFIPEVGTNFVY 300
+ G IG GH PVN AW IPA +W + E L +A + V +PEV N
Sbjct: 253 VDYGLEIGGGHGPVNPVAWQAIPAGRWEVLENLEKAGETLRQAEDVVHTLVPEVLMNIAM 312
Query: 301 SLPLPYARTRDDVAGVKGRI-VRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAI 359
++P PYART DVA GRI V +G V G EFGAS H+AR VL M P+ R+A
Sbjct: 313 AIPYPYARTPLDVAAFPGRIGVHMGRLV-FKGKPEFGASRHMARLVLAAMSVNPDIRAAG 371
Query: 360 NIRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHL 419
NI+YS++ VR AE+ GL+ DRR EP ++K REG T+PW + SA + + + PDVIY
Sbjct: 372 NIKYSKDVVRAAEDLGLLTYRGDRRAEPPDVKAREGGTLPWMLRSAYESLGRLPDVIYDE 431
Query: 420 GDVGKEPMILIFGRNPREV 438
G+VGKEPM+ I GR +V
Sbjct: 432 GEVGKEPMVRILGRTAVDV 450
>gb|AAK42540.1| Phosphomethylpyrimidine kinase (thiD-2) [Sulfolobus solfataricus]
Length = 456
Score = 243 bits (614), Expect = 3e-63
Identities = 160/444 (36%), Positives = 237/444 (53%), Gaps = 14/444 (3%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
++IAG D+G GAG E DL+ F G+HG+ AIT++TAQ+T+ + I + P+ + +QI
Sbjct: 19 MSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKGIKDITVVNPEFLKKQIET 78
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIK 127
+ ED V+A K GM+ E + V + + D+ LV DPV+ AK G PL+R+ ++ K
Sbjct: 79 LLEDFKVEAVKIGMIYTKEQFQVVNELLN--DYFLVADPVLYAKDGTPLIRD--IEEYKK 134
Query: 128 YILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAID 187
ILP ++TPN EA +SG+KI D I KK+ + VI+KGGH +G+ + D
Sbjct: 135 IILPKVKVITPNIIEASAISGVKIEKESDVVIVCKKLRDSYNIPYVIIKGGHSKGDYSFD 194
Query: 188 VLYHN-GSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAMG 246
+ ++ G Y++ THGTG F+ A+TA K L+ A A+ F+ I G
Sbjct: 195 YMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAFRKARDFVQTSIEYG 254
Query: 247 HRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPVKF---IPEVGTNFVYSLP 303
IG G PVN S I + K+ EE+ R +F E N +F IPEV +N +S+
Sbjct: 255 LNIGKGIGPVNVSVEIMKKSMKYEAVEEM-RRFADFAENND-RFWILIPEVQSNLAHSIK 312
Query: 304 LPYARTRDDVAGVKGRIVRVGNEVRAVG-PVEFGASDHLARAVLTFMKFYPEYRSAINIR 362
Y R +D+A +GRI+R ++ VG PV FG H AR +L+ + + +NIR
Sbjct: 313 PEYVRDLNDIATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLILKGKDSTCLMNIR 372
Query: 363 YSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLGDV 422
Y ++ V + G +R EP EG T+ W IE P+VIY G
Sbjct: 373 YDDKIVESFKRIGYETIEINRELEP---AHGEGKTMQWIIEYVSSEYGGIPNVIYDKGTK 429
Query: 423 GKEPMILIFGRNPREVLDKLRLLL 446
GKE MI + +N E+++ L LL
Sbjct: 430 GKEAMIRFWTKNMEEMIEALDNLL 453
>gb|AAG20645.1| (AE005133) hydroxymethylpyrimidine phosphate kinase; ThiD
[Halobacterium sp. NRC-1]
Length = 445
Score = 237 bits (599), Expect = 2e-61
Identities = 165/439 (37%), Positives = 225/439 (50%), Gaps = 12/439 (2%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIR 66
ALTIAGSDSGGGAGI+ADLKTF A G AIT+VTAQNT VT + P+ V QI
Sbjct: 5 ALTIAGSDSGGGAGIQADLKTFEAGDAFGTSAITAVTAQNTHGVTHSDILAPETVRAQID 64
Query: 67 AVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALI 126
AV D V A KTGML +A ++ AVA + P+ VDPV++++SG LL E + A+
Sbjct: 65 AVTTDFDVSAVKTGMLGDAAVVDAVATALAETPAPVTVDPVVVSQSGDRLLTESGIAAVR 124
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAI 186
+LP ATL TPN EAE L+ +I D + AA+ + E G A ++ GGH++G + +
Sbjct: 125 DELLPRATLATPNIPEAELLADTEITDETDLRAAAEAVC-ELGPDATLLTGGHLDG-DPV 182
Query: 187 DVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAM 245
DV + + + P++E THG+GC+ SAAITA LA G L A+E + I
Sbjct: 183 DV-FAGDTTSAFTRPRIETPTTHGSGCTLSAAITARLAHGDALRPAVERGIDAVAAAIDG 241
Query: 246 GHRIGHGHCPVNQSAWIEIPAEKWRIYEELTR-AVREFEEMNPVKFIPEVGTNFVYSLPL 304
+G G PV+ P R V E P +PEVG N ++
Sbjct: 242 DLSVGGGDGPVDHRHATATPTTGVGAGITAVRDVVAMLEAEWPPALVPEVGMN--VAVAP 299
Query: 305 PYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAINIRYS 364
P A T DV V GR+ VRA G +E GAS H+AR +L + +A N+R+S
Sbjct: 300 PAATTPADVVAVDGRLHATTRGVRAAGGIEPGASSHIARFLLGVRAHDADVTAATNVRWS 359
Query: 365 EETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLGDVGK 424
++ VS DR EP + T+ W + + PD + G VGK
Sbjct: 360 AAREDALGDRWDTVS-VDRTTEPAD----ADGTMDWTAQRVMADRDSAPDAVVDSGAVGK 414
Query: 425 EPMILIFGRNPREVLDKLR 443
E M+ + + + KLR
Sbjct: 415 EAMVRVLAADADALTAKLR 433
>pir||D81807 phosphomethylpyrimidine kinase (EC 2.7.4.7) NMA1815 [imported] -
Neisseria meningitidis (group A strain Z2491)
>gi|7380454|emb|CAB85040.1| (AL162757)
phosphomethylpyrimidine kinase [Neisseria meningitidis
Z2491]
Length = 268
Score = 224 bits (564), Expect = 2e-57
Identities = 129/259 (49%), Positives = 168/259 (64%), Gaps = 6/259 (2%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
LTIAGSDSGGGAGI+ADLKTF GV G IT+VTAQNT V+A+H +P + ++ QI+A
Sbjct: 9 LTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVTAQNTLGVSAVHLVPTETITAQIQA 68
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLREDAVDALI 126
+ ED + A K GML AEII+ VA +K+ DF V+DPVMIAK GAPLL++ AV AL
Sbjct: 69 IREDFDIRAYKIGMLGTAEIIECVADKLKHCDFGRRVLDPVMIAKGGAPLLQDSAVAALT 128
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEA- 185
+ +LP ++TPN EAE L+G+ I +DA+ AA KI + G K V++KGGH+ G +
Sbjct: 129 RLLLPDTDVLTPNLPEAEALTGVHIENRKDAERAA-KILLDYGVKNVVIKGGHLNGSSSG 187
Query: 186 --IDVLYHNGSYRLYRAPKVEGC-THGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLG 242
D L+ +P+ THGTGC+FSA ITA LAKG + A+ AK +IT
Sbjct: 188 RCTDWLFTQNETLELDSPRFPTAHTHGTGCTFSACITAELAKGLDVCKAVRTAKSYITAA 247
Query: 243 IAMGHRIGHGHCPVNQSAW 261
I+ IG GH PVN A+
Sbjct: 248 ISSPLEIGAGHGPVNHWAY 266
>pir||F81061 phosphomethylpyrimidine kinase NMB1616 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7226867|gb|AAF41968.1| (AE002512)
phosphomethylpyrimidine kinase [Neisseria meningitidis
MC58]
Length = 268
Score = 223 bits (562), Expect = 4e-57
Identities = 128/259 (49%), Positives = 169/259 (64%), Gaps = 6/259 (2%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
LTIAGSDSGGGAGI+ADLKTF GV G IT+VTAQNT V+A+H +P + ++ QI+A
Sbjct: 9 LTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVTAQNTLGVSAVHLVPTETITAQIQA 68
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLREDAVDALI 126
+ ED + A K GML AEII+ VA +K+ F V+DPVMIAK GAPLL++ AV AL
Sbjct: 69 IREDFDIRAYKIGMLGTAEIIECVADKLKHCSFGRRVLDPVMIAKGGAPLLQDSAVAALT 128
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGE--- 183
+ +LP ++TPN EAE L+G+ I +DA+ AA KI + G K V++KGGH+ G
Sbjct: 129 RLLLPDTDVLTPNLPEAEALTGVHIENRKDAERAA-KILLDYGVKNVVIKGGHLNGSTSG 187
Query: 184 EAIDVLYHNGSYRLYRAPKVEGC-THGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLG 242
D L+ + +P+ THGTGC+FSA ITA LAKG + +A++ AK +IT
Sbjct: 188 RCTDWLFTQNETLEFDSPRFPTAHTHGTGCTFSACITAELAKGSDVCEAVQTAKAYITAA 247
Query: 243 IAMGHRIGHGHCPVNQSAW 261
I+ IG GH PVN A+
Sbjct: 248 ISNPLEIGAGHGPVNHWAY 266
>sp|P44697|THID_HAEIN PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|1074396|pir||I64151 hypothetical protein
HI0416 - Haemophilus influenzae (strain Rd KW20)
>gi|1573390|gb|AAC22074.1| (U32725)
phosphomethylpyrimidine kinase (thiD) [Haemophilus
influenzae Rd]
Length = 269
Score = 222 bits (559), Expect = 8e-57
Identities = 137/268 (51%), Positives = 171/268 (63%), Gaps = 9/268 (3%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
M VK LTIAGSDSGGGAGI+ADLKTF GV G AIT+VTAQNT V IH IP
Sbjct: 1 MSNVKQVLTIAGSDSGGGAGIQADLKTFQMRGVFGTSAITAVTAQNTLGVFDIHPIPLKT 60
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLRE 119
+ Q+ AV D + + K GML NAEII+ VA + + F LV+DPVMIAK GAPLL+
Sbjct: 61 IQAQLEAVKNDFQIASCKIGMLGNAEIIECVADFLADKPFGTLVLDPVMIAKGGAPLLQR 120
Query: 120 DAVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKI-AAKKIAEETGAKAVIVKGG 178
AV AL + +LPLA ++TPN EAE L+G+ I V+D I A K ++ GAK VI+KGG
Sbjct: 121 QAVSALSQKLLPLADVITPNIPEAEALTGIAI--VDDISIQQAAKALQKQGAKNVIIKGG 178
Query: 179 H---IEGEEAID-VLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIE 233
H + E D VL +G + ++P+ THGTGC+FSA +TA LAKG L+ A++
Sbjct: 179 HSLNSQSELCQDWVLLADGRHFTLQSPRFNTPHTHGTGCTFSACLTAELAKGEPLQSAVK 238
Query: 234 VAKKFITLGIAMGHRIGHGHCPVNQSAW 261
AK FIT I+ IG GH P N A+
Sbjct: 239 TAKDFITAAISHPLNIGQGHGPTNHWAY 266
>pir||F69845 phosphomethylpyrimidine kinase homolog yjbV - Bacillus subtilis
>gi|2633525|emb|CAB13028.1| (Z99110) similar to
phosphomethylpyrimidine kinase [Bacillus subtilis]
Length = 271
Score = 217 bits (547), Expect = 2e-55
Identities = 122/258 (47%), Positives = 168/258 (64%), Gaps = 4/258 (1%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIR 66
ALTIAGSDSGGGAGI+AD+KTF V G+ AIT+VTAQNT V +H + + + +QI
Sbjct: 6 ALTIAGSDSGGGAGIQADIKTFQELDVFGMSAITAVTAQNTLGVHGVHPLTVETLRQQID 65
Query: 67 AVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLREDAVDAL 125
AVAED+ DA KTGML NA++I+ VA+ + Y F ++VDPVMIAK GA LLR+++V L
Sbjct: 66 AVAEDLRPDAVKTGMLWNADMIEEVARKIDEYGFNRVIVDPVMIAKGGASLLRDESVATL 125
Query: 126 IKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGH-IEGEE 184
+ ++P + +TPN EAE L+GM I +++D K AA+++ + GA+ VI+KGGH E
Sbjct: 126 KELLIPRSYAITPNVPEAETLTGMTISSLDDRKKAAEQLV-KMGAQHVIIKGGHQPEDNH 184
Query: 185 AIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGI 243
D+L+ + P + THGTGC+F+AA+TA AKG + A EVA F+ +
Sbjct: 185 ITDLLFDGSMFMQITHPYINTKHTHGTGCTFAAALTAQTAKGDSIHQAFEVAANFVREAV 244
Query: 244 AMGHRIGHGHCPVNQSAW 261
IG GH P N A+
Sbjct: 245 ENTLGIGSGHGPTNHFAF 262
>emb|CAC12405.1| (AL445067) probable phosphomethylpyrimidine kinase [Thermoplasma
acidophilum]
Length = 263
Score = 217 bits (546), Expect = 3e-55
Identities = 116/253 (45%), Positives = 170/253 (66%), Gaps = 4/253 (1%)
Query: 3 LVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVS 62
+V ALT+AGSDSGGGAGI+ADLKTF A V G+ I ++TAQN+ V+ I +PPD V
Sbjct: 1 MVPHALTVAGSDSGGGAGIQADLKTFNALSVFGMSVIVALTAQNSYEVSGILPVPPDFVK 60
Query: 63 EQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFP-LVVDPVMIAKSGAPLLREDA 121
Q VA+DI VD +KTGML +++II VA+ + P +VVDPVM++KS A LL+EDA
Sbjct: 61 LQFETVAKDIKVDGSKTGMLFSSDIISVVAREFTEFGVPVIVVDPVMVSKSNARLLKEDA 120
Query: 122 VDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIE 181
++ L + +LP++T+VTPN EAE LSG+KIR +ED AA+K+ +ETG+ +++KGGH+E
Sbjct: 121 IETLKEKLLPISTMVTPNVPEAEILSGIKIRNIEDMHSAAEKLYDETGSH-ILIKGGHLE 179
Query: 182 GEEAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT 240
IDVLY S+ + ++ THGTG + S+AI + + +G +++ +AK+++
Sbjct: 180 -SSPIDVLYDGSSFHEFPGSRINTKNTHGTGDTLSSAIVSYMIRGHSESESVRLAKEYVE 238
Query: 241 LGIAMGHRIGHGH 253
I G G+
Sbjct: 239 GAIKNSFTTGKGY 251
>dbj|BAB05154.1| (AP001512) phosphomethylpyrimidine kinase [Bacillus halodurans]
Length = 270
Score = 216 bits (545), Expect = 3e-55
Identities = 121/263 (46%), Positives = 166/263 (63%), Gaps = 2/263 (0%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
M + ALTIAGSDSGGGAGI+AD+KTF G+ IT++TAQNT V ++ D
Sbjct: 1 MNNLAKALTIAGSDSGGGAGIQADIKTFQELDTFGMSVITALTAQNTLGVHGVYPQTLDA 60
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLRE 119
+ Q+ AV DIG DA KTGML +A+II VA+ + Y +VVDPVMIAK GA LL +
Sbjct: 61 IEAQLDAVLSDIGADAVKTGMLFSADIITLVAQKLTAYRVNHIVVDPVMIAKGGAALLLD 120
Query: 120 DAVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGH 179
+AV+AL+K +LP+AT++TPN EA K+ G++ A K G + V++KGGH
Sbjct: 121 EAVEALVKKLLPIATVITPNLPEAAKILGIESIDSRTEMEQAVKELHALGPRYVVLKGGH 180
Query: 180 IEGEEAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKF 238
+ G+EA D+LY + ++ + + + THGTGC+F+AAI A LAKG + DA++ AK F
Sbjct: 181 LSGDEATDLLYDGETIHVFSSSRFDTKHTHGTGCTFAAAIAAELAKGASVFDAVKTAKAF 240
Query: 239 ITLGIAMGHRIGHGHCPVNQSAW 261
I+ I IG G P N A+
Sbjct: 241 ISTAIKHAVAIGGGIGPTNHHAY 263
>sp|P56904|THID_RHIME PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|5690190|gb|AAD46983.1| (AF070520) protein
kinase homolog Thi [Sinorhizobium meliloti]
Length = 266
Score = 215 bits (543), Expect = 6e-55
Identities = 121/254 (47%), Positives = 162/254 (63%), Gaps = 3/254 (1%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIR 66
AL+IAGSDSGGGAGI+ADLKTF+A GV+G IT++TAQNT+ VTA+ D+ ++VS Q+
Sbjct: 5 ALSIAGSDSGGGAGIQADLKTFSALGVYGASVITAITAQNTRGVTAVEDVSAEIVSAQMD 64
Query: 67 AVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALI 126
AV D+ V A K GM+S E I A+A ++ + VVDPVM+A SG L R DAV ALI
Sbjct: 65 AVFSDLDVKAVKIGMVSRRETIAAIADGLRRFGRLAVVDPVMVATSGDALFRPDAVAALI 124
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAI 186
+ +LPLA + TPN EA ++G I E + TGA AV+VKGGH++G+EA
Sbjct: 125 EELLPLALVATPNLAEAALMTGRAIAGDEAEMARQAEAIMRTGAHAVLVKGGHLKGQEAT 184
Query: 187 DVLYHNGSYRLYRAPKVEGCT-HGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAM 245
D+ + + A ++E HGTGC+ SAAI A LAKG L +A+ AK ++ I+
Sbjct: 185 DLFFDGDTLVRLPAGRIETRNDHGTGCTLSAAIAAGLAKGVPLIEAVSAAKAYLHAAISA 244
Query: 246 GHR--IGHGHCPVN 257
R IG G PV+
Sbjct: 245 ADRLEIGQGRGPVH 258
>gb|AAF76451.1|AF077841_2 (AF077841) hydroxymethylpyrimidine-phosphate kinase [Sulfolobus
solfataricus]
Length = 399
Score = 215 bits (542), Expect = 8e-55
Identities = 140/392 (35%), Positives = 211/392 (53%), Gaps = 11/392 (2%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
++IAG D+G GAG E DL+ F G+HG+ AIT++TAQ+T+ + I + P+ + +QI
Sbjct: 7 MSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKGIKDITVVNPEFLKKQIET 66
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIK 127
+ ED V+A K GM+ E + V + + D+ LV DPV+ AK G PL+R+ ++ K
Sbjct: 67 LLEDFKVEAVKIGMIYTKEQFQVVNELLN--DYFLVADPVLYAKDGTPLIRD--IEEYKK 122
Query: 128 YILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAID 187
ILP ++TPN EA +SG+KI D I KK+ + VI+KGGH +G+ + D
Sbjct: 123 IILPKVKVITPNIIEASAISGVKIEKESDVVIVCKKLRDSYNIPYVIIKGGHSKGDYSFD 182
Query: 188 VLYHN-GSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAMG 246
+ ++ G Y++ THGTG F+ A+TA K L+ A A+ F+ I G
Sbjct: 183 YMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAFRKARDFVQTSIEYG 242
Query: 247 HRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPVKF---IPEVGTNFVYSLP 303
IG G PVN S I + K+ EE+ R +F E N +F IPEV +N +S+
Sbjct: 243 LNIGKGIGPVNVSVEIMKKSMKYEAVEEM-RRFADFAENND-RFWILIPEVQSNLAHSIK 300
Query: 304 LPYARTRDDVAGVKGRIVRVGNEVRAVG-PVEFGASDHLARAVLTFMKFYPEYRSAINIR 362
Y R +D+A +GRI+R ++ VG PV FG H AR +L+ + + +NIR
Sbjct: 301 PEYVRDLNDIATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLILKGKDSTCLMNIR 360
Query: 363 YSEETVRLAEEQGLVVSFYDRREEPEEIKERE 394
Y ++ V + G +R KER+
Sbjct: 361 YDDKIVESFKRIGYETIEINRELNLPMEKERQ 392
>sp|Q57688|Y236_METJA HYPOTHETICAL PROTEIN MJ0236 >gi|2128191|pir||E64329 hypothetical
protein MJ0236 - Methanococcus jannaschii
>gi|1590970|gb|AAB98222.1| (U67479) conserved
hypothetical [Methanococcus jannaschii]
Length = 421
Score = 213 bits (536), Expect = 4e-54
Identities = 143/450 (31%), Positives = 238/450 (52%), Gaps = 42/450 (9%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
M ++ L I G D GAGI AD+KT GV+ TSV QN + V D+P +
Sbjct: 1 MVILMVILAIGGYDPTSGAGISADIKTAHTLGVYCPTITTSVIPQNNKMVYEKFDLPEEN 60
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLRED 120
+ Q +AV E+ ++ KTG+L+ I + K + YD ++ DPV+ + + + E
Sbjct: 61 IKNQFKAVFEEFDIEYVKTGVLTK-PAIDTLLKYIDKYDLKVICDPVLASTTKFSFVDEK 119
Query: 121 AVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI 180
++ I+ + + L+TPN+ E +K+ M+ + I TG +++K
Sbjct: 120 LMEKYIE-LFNKSFLITPNKEEYKKI--MEFIKNNNLMIRNDLYILATGIDDILMK---- 172
Query: 181 EGEEAIDVLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT 240
+ + ++ +V+ HGTGC +S AITA L+KG+ LE+AI+ AK+F+
Sbjct: 173 ----------NFKPIKTFKGFRVDKEVHGTGCVYSTAITAFLSKGYDLEEAIKEAKRFVL 222
Query: 241 LGI--AMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFEEMNPVKFIPEVGTNF 298
+ A + G+ P K ++ + L+ A+ ++MN IPEVG+N
Sbjct: 223 SSVIYAKKSKFGYNSNPTY--------INKEKVIKNLSYAIYLLKKMN-FTLIPEVGSNI 273
Query: 299 VYSLPLPYARTRDDVAGVKGRIVRVGNEVRA---VGPVEFGASDHLARAVLTFMKFYPEY 355
SLP P + DVA + GRI++ N++ VG +EFGAS+H+A+ +L+ KF PE
Sbjct: 274 AESLPFP--KDFKDVAALTGRIIK--NKLGGFYIVGDIEFGASEHIAKIILSASKFNPEI 329
Query: 356 RSAINIRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDV 415
R+ +NI+Y ++L +++ VS +DR+EEP + +T+ WG + A ++ PD+
Sbjct: 330 RACMNIKYDGGLIKLLKDK-FAVSSFDRKEEPPNV-----STMEWGTKIACEKFGGVPDI 383
Query: 416 IYHLGDVGKEPMILIFGRNPREVLDKLRLL 445
IY G GKEPMI + GR+ EV+ K+ ++
Sbjct: 384 IYDRGGEGKEPMIRVLGRDAIEVVKKVEVI 413
>gb|AAF25542.1|AF109218_2 (AF109218) ThiD [Staphylococcus carnosus]
Length = 273
Score = 206 bits (518), Expect = 5e-52
Identities = 116/266 (43%), Positives = 166/266 (61%), Gaps = 3/266 (1%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
M K ALTIAG+D GGAG+ ADLK+F A GV+G+ AITS+ AQNT+ V IH++
Sbjct: 1 MNKPKIALTIAGTDPTGGAGVMADLKSFHACGVYGMAAITSIVAQNTKGVQHIHNLDITW 60
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNY-DFPLVVDPVMIAKSGAPLLRE 119
+ EQ+ ++ +D A KTGM++ E+++ + ++ Y D P V+DPVM+AKSG L+ +
Sbjct: 61 LKEQLDSIFDDELPQAIKTGMIATKEMMELIRSYLEKYPDIPYVIDPVMLAKSGDSLMDD 120
Query: 120 DAVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGH 179
AL + +LPLA + TPN EAE++ G K+ T E K A E G+K V++KGGH
Sbjct: 121 AGKHALQEILLPLADVATPNLPEAEEIVGFKLDTEEAIKKAGDIFINEIGSKGVVIKGGH 180
Query: 180 IEGEE-AIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKK 237
IE + A D L+ ++ + + + THGTGC+FSA ITA LAKG + +A++ AK
Sbjct: 181 IEDKNIAKDYLFTKDGLEVFESERYDTKHTHGTGCTFSAVITAELAKGKTIYEAVKKAKD 240
Query: 238 FITLGIAMGHRIGHGHCPVNQSAWIE 263
FI L I IG G PVN A+++
Sbjct: 241 FIALSIKYTPEIGQGRGPVNHFAYMK 266
>sp|O67772|THID_AQUAE PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|7449914|pir||H70467 HMP-P kinase - Aquifex
aeolicus >gi|2984203|gb|AAC07733.1| (AE000765) HMP-P
kinase [Aquifex aeolicus]
Length = 255
Score = 205 bits (517), Expect = 7e-52
Identities = 112/249 (44%), Positives = 162/249 (64%), Gaps = 3/249 (1%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIR 66
AL+IAG D+ GGAG AD++TF FG++G+ IT++ QNTQ V + IPPDVV E+++
Sbjct: 3 ALSIAGFDNSGGAGTLADIRTFKHFGIYGVAVITALAVQNTQKVYEVFPIPPDVVKEELK 62
Query: 67 AVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFP-LVVDPVMIAKSGAPLLREDAVDAL 125
A+ ED + K GML+N EI + V +T+K+ +V+DPV +KSG LL E+ V+ L
Sbjct: 63 AIFEDFPIKGVKIGMLANKEIAEVVYETLKSKKTNFIVLDPVFRSKSGRELLSEEGVEFL 122
Query: 126 IKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEA 185
+ + L+TPN EAE L G +I+++ED K A+KI GAK+V++KGGH++G A
Sbjct: 123 KSEFIKIVDLITPNVPEAEILCGEEIKSLEDVKNCAQKI-YSLGAKSVLIKGGHLKGNYA 181
Query: 186 IDVLYHNGSYRLYRAPKVEGCT-HGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIA 244
ID+LY S+ ++APK+ G T GTGC +S+AI AN + L AI+ AK FIT I
Sbjct: 182 IDILYDGKSFYEFKAPKIAGKTPRGTGCVYSSAILANYLRHKDLIKAIKTAKDFITEAIK 241
Query: 245 MGHRIGHGH 253
++G G+
Sbjct: 242 NSKKLGKGY 250
>sp|Q9ZBR6|THID_STRCO PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|7480322|pir||T35647 phosphomethylpyrimidine
kinase - Streptomyces coelicolor
>gi|4007722|emb|CAA22406.1| (AL034447)
phosphomethylpyrimidine kinase [Streptomyces coelicolor
A3(2)]
Length = 269
Score = 205 bits (515), Expect = 1e-51
Identities = 107/251 (42%), Positives = 162/251 (63%), Gaps = 3/251 (1%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
LT+AGSDSGGGAGI+ADLKT A G HG+ +T+VTAQN++ V ++P + V Q R+
Sbjct: 14 LTVAGSDSGGGAGIQADLKTMLALGTHGMSVLTAVTAQNSRGVQGAWELPVEAVRAQYRS 73
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIK 127
V +DIGV A KTGMLS+AE+++ VA+ + D P VVDPV ++K G LL A++++
Sbjct: 74 VVDDIGVQAVKTGMLSSAELVETVAELLAGTDAPAVVDPVGVSKHGDALLASSALESVRT 133
Query: 128 YILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAID 187
+LP+AT+ TPN E +L+G+++ D + AA + G + ++KGGH+ G +A+D
Sbjct: 134 RLLPVATVATPNLDEVAQLTGVRVDDETDLRRAAAAVL-AFGPRWALIKGGHLAG-DAVD 191
Query: 188 VLYHNGSYRLYRAPKVEG-CTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAMG 246
+L RAP+++ THGTGC+ ++A+ LAKG + A+ AK+++T I G
Sbjct: 192 LLTDGSEEHWLRAPRLDNRHTHGTGCTLASAVACGLAKGQSVPVAVRAAKEYVTGAITAG 251
Query: 247 HRIGHGHCPVN 257
+G G PV+
Sbjct: 252 FPLGGGIGPVD 262
>pir||D82784 phosphomethylpyrimidine kinase XF0621 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9105493|gb|AAF83431.1|AE003907_9 (AE003907)
phosphomethylpyrimidine kinase [Xylella fastidiosa]
Length = 274
Score = 204 bits (514), Expect = 2e-51
Identities = 117/254 (46%), Positives = 162/254 (63%), Gaps = 5/254 (1%)
Query: 6 TALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQI 65
+ALTIAGSD+GGGAGI+ADLKTF A +HGL AIT +TAQ+T+ V A+H P + ++ QI
Sbjct: 13 SALTIAGSDTGGGAGIQADLKTFAAHHIHGLSAITVLTAQHTRGVNAVHIPPLEFIATQI 72
Query: 66 RAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFP-LVVDPVMIAKSGAPLLREDAVDA 124
D V A K GML+N E+I VA+ ++ + P +V+DPVMIA SG+ LL + A++A
Sbjct: 73 DTCFSDFNVHAVKLGMLANTEVITLVAEALQRHQPPCIVLDPVMIATSGSTLLEDSALEA 132
Query: 125 LIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI-EGE 183
L +LPLATL+TPN EAE+L I + DA A + + GA AV++KGGH+ EG
Sbjct: 133 LRTQLLPLATLITPNIPEAEQLLNRHIHSSSDADTATASLL-KLGAHAVLLKGGHLNEGN 191
Query: 184 EAIDVLYHNGSYR-LYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLG 242
ID + NG+ R + P++ HGTGC+ S+AITA L G L A + ++ G
Sbjct: 192 SVID-RFDNGTLRERFSNPRLALNAHGTGCTLSSAITAQLCLGLNLHQACKAGIDYVARG 250
Query: 243 IAMGHRIGHGHCPV 256
+ G+R G G+ V
Sbjct: 251 LHAGYRPGRGNITV 264
>sp|P96268|THID_MYCTU PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|7449916|pir||D70630 probable
PHOSPHOMETHYLPYRIMIDINE KINASE - Mycobacterium
tuberculosis (strain H37RV) >gi|1817688|emb|CAB06562.1|
(Z84724) thiD [Mycobacterium tuberculosis]
Length = 265
Score = 201 bits (506), Expect = 1e-50
Identities = 114/254 (44%), Positives = 160/254 (62%), Gaps = 5/254 (1%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
L+IAGSDSGGGAGI+AD++T GVH VA+T+VT QNT V IH++P DVV+ QI A
Sbjct: 7 LSIAGSDSGGGAGIQADMRTMALLGVHACVAVTAVTVQNTLGVKDIHEVPNDVVAGQIEA 66
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF--PLVVDPVMIAKSGAPLLREDAVDAL 125
V DIGV AAKTGML+++ I+ VA T + + PLVVDPV + G PLL A+D+L
Sbjct: 67 VVTDIGVQAAKTGMLASSRIVATVAATWRRLELSVPLVVDPVCASMHGDPLLAPSALDSL 126
Query: 126 IKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEE- 184
+ PLATL+TPN EA L +++ E + AAK + G + V+VKGGH+ +
Sbjct: 127 RGQLFPLATLLTPNLDEARLLVDIEVVDAESQRAAAKAL-HALGPQWVLVKGGHLRSSDG 185
Query: 185 AIDVLYHNGSYRLYRAPKV-EGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGI 243
+ D+LY S + A ++ G HG G + + AI A LA GF + DA++ K+++T +
Sbjct: 186 SCDLLYDGVSCYQFDAQRLPTGDDHGGGDTLATAIAAALAHGFTVPDAVDFGKRWVTECL 245
Query: 244 AMGHRIGHGHCPVN 257
+ +G GH PV+
Sbjct: 246 RAAYPLGRGHGPVS 259
>sp|O85786|THID_SYNP7 PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|11280142|pir||T51093 thiamin biosynthesis
protein [imported] - Synechococcus sp. (PCC 7942)
>gi|3319338|gb|AAC26228.1| (AF076530) putative thiamine
biosynthesis protein [Synechococcus PCC7942]
Length = 263
Score = 198 bits (498), Expect = 1e-49
Identities = 112/255 (43%), Positives = 157/255 (60%), Gaps = 3/255 (1%)
Query: 4 VKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSE 63
V ALTIAGSDSGGGAGI+ADL+TF VHG AIT VTAQNT VT + I P V+
Sbjct: 3 VPIALTIAGSDSGGGAGIQADLRTFAFHQVHGTCAITCVTAQNTLGVTRVDAIAPAGVAA 62
Query: 64 QIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
Q+ AV D+ A KTGML NAEI++ VA T+ P ++DPVM++++GA L+ + A+
Sbjct: 63 QLDAVLSDLPPQALKTGMLLNAEIMEVVAGTIAPLFIPRIIDPVMVSRTGAVLIDQAAIA 122
Query: 124 ALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGE 183
L ++LPLAT++TPNR+EA+ L+GM I+ D AA +I + G +AV++KGG G+
Sbjct: 123 VLRDHLLPLATVLTPNRYEAQLLAGMDIQDAADLDRAA-RIIRDLGPQAVLIKGGAATGD 181
Query: 184 -EAIDVLYHNGSYRLYRAPKV-EGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITL 241
+D + + + + THG+GC+ +AAI AN A G + A + AK ++T
Sbjct: 182 WHGVDWWWDGQQSHILKTEAIATPHTHGSGCTLAAAIAANAALGLEILAAAQAAKHYVTK 241
Query: 242 GIAMGHRIGHGHCPV 256
IG G P+
Sbjct: 242 AFRHSFAIGQGQGPL 256
>sp|Q9ZBL1|THID_MYCLE PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|11280143|pir||T44742 probable
phosphomethylpyrimidine kinase (EC 2.7.-.-) thiD
[imported] - Mycobacterium leprae
>gi|4154057|emb|CAA22711.1| (AL035159) putative
phosphomethylpyrimidine kinase ThiD [Mycobacterium
leprae]
Length = 279
Score = 197 bits (495), Expect = 3e-49
Identities = 110/258 (42%), Positives = 162/258 (62%), Gaps = 9/258 (3%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
L+IAGSDSGGGAGI+AD++T T GVH A+T+VT QNT V H+IP ++V+ QI+A
Sbjct: 17 LSIAGSDSGGGAGIQADMRTMTVLGVHACTAVTAVTIQNTLGVEGFHEIPAEIVASQIKA 76
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF--PLVVDPVMIAKSGAPLLREDAVDAL 125
V DIG+ +AKTGML+++ II AVA+T + PLVVDPV + G PLL +A+D+L
Sbjct: 77 VVTDIGIQSAKTGMLASSSIIAAVAETWLRLELTTPLVVDPVCASMHGDPLLTGEAMDSL 136
Query: 126 IKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI-EGEE 184
+ PLAT+VTPN E L G+ + E + AA + G + +VKGGH+ +
Sbjct: 137 RDRLFPLATVVTPNLDEVRLLVGIDVVDTESQRAAAMAL-HALGPQWALVKGGHLRSSDR 195
Query: 185 AIDVLYHNGS----YRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFI 239
+ D+LY S + + AP+V+ G HG G + +AA++ LA G+ + DA+ K+++
Sbjct: 196 SCDLLYGGSSAGVAFHEFDAPRVQTGNDHGGGDTLAAAVSCALAHGYTVPDAVGFGKRWV 255
Query: 240 TLGIAMGHRIGHGHCPVN 257
T + + +G GH PV+
Sbjct: 256 TECLRDAYPLGRGHGPVS 273
>gb|AAK40369.1| Phosphomethylpyrimidine kinase (thiD-1) [Sulfolobus solfataricus]
Length = 400
Score = 194 bits (489), Expect = 1e-48
Identities = 131/372 (35%), Positives = 212/372 (56%), Gaps = 28/372 (7%)
Query: 9 TIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRAV 68
TIAGSDSGGGAG++ADLKTF+A GV G IT +TAQNT+ VT + +IP D + Q AV
Sbjct: 9 TIAGSDSGGGAGLQADLKTFSALGVFGTTIITGLTAQNTRTVTKVLEIPLDFIEAQFDAV 68
Query: 69 AEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIKY 128
D+ AKTGML++ ++++ V + ++ Y+ LV+DPVM+AKSG+ L+ ED + IK
Sbjct: 69 CLDLHPTHAKTGMLASGKVVELVLRKIREYNIKLVLDPVMVAKSGSLLVTED-ISEQIKK 127
Query: 129 ILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGH------IEG 182
+ A + TPNR+EAE ++ KI + +D AA++I + G V+VKG + I+G
Sbjct: 128 AMKEAIISTPNRYEAEIINKTKINSQDDVIKAAREIYSKYG--NVVVKGFNGVDYAIIDG 185
Query: 183 EEAIDVLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLG 242
EE + G Y + THG+G FSA+ITA LA G+ L+DA+ AKKF T+
Sbjct: 186 EE----IELKGDYISTKN------THGSGDVFSASITAYLALGYKLKDALIRAKKFATMT 235
Query: 243 IAMGHRIGHGHCPVNQSAWIEIPAEKWRIYEELTRAVREFE-EMNPVKFIPEVGTNFVYS 301
+ G +G G+ PV+ A IE ++ +L + E +N + + N
Sbjct: 236 VKYGLDLGGGYGPVDPFAPIESIVKREEGRNQLENLLWYLESNLNVILKL----INDTSK 291
Query: 302 LPLPYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAINI 361
+ + Y +DV + G +++ ++++ GP+ ++ + R M+ P+ + I +
Sbjct: 292 VNVAYMTKYNDVLSLAGGLIKYLDKLKIDGPILNNVNNEITR----IMRETPDEKIGILL 347
Query: 362 RYSEETVRLAEE 373
++++ + AE+
Sbjct: 348 PFTDKILDAAEK 359
>sp|Q9ZL00|THID_HELPJ PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|7449908|pir||H71889 phosphomethylpyrimidine
kinase - Helicobacter pylori (strain J99)
>gi|4155349|gb|AAD06362.1| (AE001508)
PHOSPHOMETHYLPYRIMIDINE KINASE [Helicobacter pylori J99]
Length = 269
Score = 193 bits (486), Expect = 3e-48
Identities = 113/262 (43%), Positives = 160/262 (60%), Gaps = 6/262 (2%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
M++ L+IAGSDSGGGAGI+ADLK F FGV G IT +TAQNTQ V ++ + +
Sbjct: 1 MKVYPQVLSIAGSDSGGGAGIQADLKAFQTFGVFGTSVITCITAQNTQGVHGVYPLSVES 60
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPL-VVDPVMIAKSGAPLLRE 119
V QI A+ +D + A K G L NA+II+ VA ++ DF L V+DPVM+AK+GA LL E
Sbjct: 61 VKAQILAIRDDFSIKAFKMGALCNAQIIECVANALETCDFGLCVLDPVMVAKNGALLLEE 120
Query: 120 DAVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGH 179
+A+ +L K +LP L+TPN E L+G++ R + A A + + G K ++KGGH
Sbjct: 121 EAILSLKKRLLPKTNLLTPNLPEVYALTGVQARDDKSAS-KAMGVLRDLGVKNAVIKGGH 179
Query: 180 IE---GEEAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVA 235
E GE + D ++ + + A + THGTGC+ S+ I LA+G L++AI A
Sbjct: 180 TEHFQGEFSNDWVFLEDAEFILSAKRFNTKNTHGTGCTLSSLIVGLLAQGLDLKNAITKA 239
Query: 236 KKFITLGIAMGHRIGHGHCPVN 257
K+ +T+ I IGHGH P+N
Sbjct: 240 KELLTIIIQNPLNIGHGHGPLN 261
>sp|O25515|THID_HELPY PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|7449910|pir||D64625 thiamin biosynthesis
protein - Helicobacter pylori (strain 26695)
>gi|2313975|gb|AAD07890.1| (AE000595) thiamine
biosynthesis protein (thi) [Helicobacter pylori 26695]
Length = 270
Score = 192 bits (484), Expect = 5e-48
Identities = 111/262 (42%), Positives = 161/262 (61%), Gaps = 6/262 (2%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
+++ L+IAGSDSGGG+GI+ADLK F GV G IT +TAQNTQ V ++ + +
Sbjct: 2 VKIYPQVLSIAGSDSGGGSGIQADLKAFQTLGVFGTSVITCITAQNTQGVHGVYPLSVES 61
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPL-VVDPVMIAKSGAPLLRE 119
V QI A+ +D + A K G L NA+II+ VA T++ DF L V+DPVM+AK+GA LL E
Sbjct: 62 VKAQILAIRDDFSIKAFKMGALCNAQIIECVADTLETCDFGLCVLDPVMVAKNGALLLEE 121
Query: 120 DAVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGH 179
+A+ +L K +LP L+TPN E L+G+++R + A A + + G K ++KGGH
Sbjct: 122 EAILSLKKRLLPTTHLLTPNLPEVYALTGVQVRDDKSAS-KAMGVLRDLGVKNAVIKGGH 180
Query: 180 IE---GEEAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVA 235
E GE + D ++ + + A + THGTGC+ S+ I LA+G L++AI A
Sbjct: 181 TEHFQGEYSNDWVFLEDAEFILNAKRFNTKNTHGTGCTLSSLIVGLLAQGLDLKNAISKA 240
Query: 236 KKFITLGIAMGHRIGHGHCPVN 257
K+ +T+ I IGHGH P+N
Sbjct: 241 KELLTIIIQNPLNIGHGHGPLN 262
>sp|P39610|THID_BACSU PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|629059|pir||S39707 phosphomethylpyrimidine
kinase thiD - Bacillus subtilis
>gi|413976|emb|CAA51608.1| (X73124) ipa-52r [Bacillus
subtilis] >gi|2636337|emb|CAB15828.1| (Z99123)
phosphomethylpyrimidine kinase [Bacillus subtilis]
Length = 271
Score = 191 bits (480), Expect = 1e-47
Identities = 112/259 (43%), Positives = 155/259 (59%), Gaps = 5/259 (1%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQ--NTQAVTAIHDIPPDVVSEQ 64
ALTIAGSDS GGAGI+ADLKTF V+G+ A+T + A N + I D + Q
Sbjct: 6 ALTIAGSDSSGGAGIQADLKTFQEKNVYGMTALTVIVAMDPNNSWNHQVFPIDTDTIRAQ 65
Query: 65 IRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFP-LVVDPVMIAKSGAPLLREDAVD 123
+ + + IGVDA KTGML +II+ AKT+K +V+DPVM+ K +L +
Sbjct: 66 LATITDGIGVDAMKTGMLPTVDIIELAAKTIKEKQLKNVVIDPVMVCKGANEVLYPEHAQ 125
Query: 124 ALIKYILPLATLVTPNRFEAEKLSGM-KIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEG 182
AL + + PLAT++TPN FEA +LSGM +++TV+D AAKKI VI GG ++
Sbjct: 126 ALREQLAPLATVITPNLFEASQLSGMDELKTVDDMIEAAKKIHALGAQYVVITGGGKLKH 185
Query: 183 EEAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITL 241
E+A+DVLY + + + ++ THG GC+FSAA+TA LAKG +++AI AK+FIT
Sbjct: 186 EKAVDVLYDGETAEVLESEMIDTPYTHGAGCTFSAAVTAELAKGAEVKEAIYAAKEFITA 245
Query: 242 GIAMGHRIGHGHCPVNQSA 260
I + P SA
Sbjct: 246 AIKESFPLNQYVGPTKHSA 264
>pir||F69181 transcription regulator - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2621694|gb|AAB85120.1| (AE000842)
transcriptional regulator [Methanobacterium
thermoautotrophicum]
Length = 253
Score = 191 bits (480), Expect = 1e-47
Identities = 112/242 (46%), Positives = 152/242 (62%), Gaps = 7/242 (2%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIR 66
AL+IAG D GGAGI AD+KTF+A GV+G IT++TAQN V+ I +PP V EQ+
Sbjct: 3 ALSIAGFDPSGGAGILADIKTFSALGVYGAGVITALTAQNVGRVSGIMPVPPGFVEEQLD 62
Query: 67 AVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALI 126
V ED+ V AKTGML +AEI++AVA+ V+ Y ++VDPVM+A SG L + V AL
Sbjct: 63 LVMEDLPVVHAKTGMLYSAEIVEAVAEKVREYGLRVIVDPVMVAASGGELSEGNFVRALR 122
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAI 186
+++LP A +VTPN EAE+LS + I TV+DA AA+ I E VI+ GGH+ G I
Sbjct: 123 RHLLPEALIVTPNVAEAERLSDIPIGTVDDAIRAARVIGELCD---VIITGGHLGGRSVI 179
Query: 187 DVLYHNGSYRLYRAPKVEG-CTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAM 245
+ +G + ++ THG+GCSFSAA A L +G L +A+ +A F+ I
Sbjct: 180 LI---DGDVEVLEGKLLDSRNTHGSGCSFSAATAAYLERGLDLGEALRMADDFVREAIRH 236
Query: 246 GH 247
GH
Sbjct: 237 GH 238
>pir||G72333 conserved hypothetical protein - Thermotoga maritima (strain MSB8)
>gi|4981319|gb|AAD35872.1|AE001747_15 (AE001747)
conserved hypothetical protein [Thermotoga maritima]
Length = 398
Score = 188 bits (472), Expect = 1e-46
Identities = 140/442 (31%), Positives = 217/442 (48%), Gaps = 51/442 (11%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
L +AG D GGAGI D+K +A GV I+++T QN V +++ + + ++I
Sbjct: 3 LVVAGFDPSGGAGIIQDVKVLSALGVKTHAVISALTVQNENRVFSVNFRDWEEMRKEIEV 62
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVDALIK 127
+ + K G LS E +K + + D +V + V+ + SG + V ++
Sbjct: 63 LTPPRVI---KVG-LSAPETVKRLREMFP--DSAIVWNVVLESSSGFGFQDPEEVKKFVE 116
Query: 128 YILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAID 187
Y A V N EA+KL IV GGH +G +
Sbjct: 117 Y----ADYVILNSEEAKKLGEYN---------------------NFIVTGGHEKGN-TVK 150
Query: 188 VLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAMGH 247
V Y + + + P+V G HGTGC+FS+A++ LA + +E+AI A + + +
Sbjct: 151 VKYRDFVFEI---PRVPGEFHGTGCAFSSAVSGFLAMSYPVEEAIRSAMELLKKILERSS 207
Query: 248 RIGHGHCPVNQSAWIEIPAEKWRIYEELT---RAVREFEEMNPVKFIPEVGTNFVYSLPL 304
+ E W Y+ L + EF E+ + +PEVG N Y+LP
Sbjct: 208 GVVE----------TEKLLRDWYRYDTLNTLDEILPEFLEIGHLT-VPEVGQNVSYALP- 255
Query: 305 PYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAINIRYS 364
+A+ +V GRI + AV F H AR +T M+++P R +N+RY
Sbjct: 256 -WAKNEFEVGKFPGRIRLKEGKAVAVSCASFKDRSHTARMAVTMMRYHPHMRCVVNVRYE 314
Query: 365 EETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLGDVGK 424
E V A+++GL V YDR +EP+E++E+EG ++ W IE AI +K PDVIY G GK
Sbjct: 315 REYVERAKKRGLKVFHYDRSKEPKEVQEKEGQSMVWMIEQAIAELKSPPDVIYDEGWWGK 374
Query: 425 EPMILIFGRNPREVLDKLRLLL 446
E MI +FGRNP+EVL+K++L++
Sbjct: 375 EAMIRVFGRNPKEVLEKIKLMV 396
>pir||D82217 phosphomethylpyrimidine kinase VC1296 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9655784|gb|AAF94455.1|
(AE004209) phosphomethylpyrimidine kinase [Vibrio
cholerae]
Length = 282
Score = 186 bits (467), Expect = 5e-46
Identities = 112/255 (43%), Positives = 160/255 (61%), Gaps = 5/255 (1%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
LTIAGSDSGGGAGI+AD+K +A G + IT++TAQNTQ V+A+ +P VV +Q+ A
Sbjct: 13 LTIAGSDSGGGAGIQADIKAISATGGYACSVITAITAQNTQGVSAVFPLPLAVVEQQLDA 72
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFP-LVVDPVMIAKSGAPLLREDAVDALI 126
V DI V A K GML++A II VAK ++ Y +V+DPVMI+ SG LL +AV+ L
Sbjct: 73 VFSDIPVLAVKIGMLADAPIIALVAKKLRQYQPKWIVLDPVMISTSGHHLLAAEAVETLK 132
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGE-EA 185
+ +LPLA L+TPN EA L+GM + + + + AV++KGGH + +
Sbjct: 133 RELLPLANLITPNLPEAAVLAGMTQQEWQQNPQQGITVLSQLNTPAVLLKGGHDHQQANS 192
Query: 186 IDVLYHNGSYRLYRAPKV-EGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIA 244
D+L +G R + AP++ THGTGCS S+AI + LA+G L +A++ AK++I I
Sbjct: 193 DDLLIQSGDSRHFCAPRIATKNTHGTGCSLSSAIASYLAQGAELVEAVDKAKQYIHQAIV 252
Query: 245 MGH--RIGHGHCPVN 257
RIG G+ P++
Sbjct: 253 HADQLRIGQGYGPIH 267
>pir||E81311 phosphomethylpyrimidine kinase (EC 2.7.4.7) Cj1082c [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6968516|emb|CAB73337.1| (AL139077)
phosphomethylpyrimidine kinase [Campylobacter jejuni]
Length = 270
Score = 184 bits (462), Expect = 2e-45
Identities = 110/252 (43%), Positives = 150/252 (58%), Gaps = 4/252 (1%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
LTIAGSD GGAGI+ADLKTF+A + G+ + SV A+NT V ++HDIP V EQ+ A
Sbjct: 12 LTIAGSDCSGGAGIQADLKTFSAHNLFGMSVVLSVVAENTARVISVHDIPTQSVDEQMLA 71
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNY-DFPLVVDPVMIAKSGAPLLREDAVDALI 126
V EDI A K GM+ + E++ VAK + + +V+DPVM AK+G L+ ++ D
Sbjct: 72 VFEDIVPKATKIGMIGSCELMSCVAKNLSEFKPQNIVIDPVMFAKNGYALMPQENCDFFK 131
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAI 186
+ I+ A ++TPN EAE L G KI E AAK + GAKAV++KGGH E A
Sbjct: 132 QTIVKFADILTPNIPEAEFLCGFKIANEEQMIKAAKHLC-SLGAKAVLLKGGHSE-INAN 189
Query: 187 DVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAM 245
DV Y + + ++E THGTGC+ S+AI +NLAKG L A+ AK+++ I
Sbjct: 190 DVFYDGKEIYILKGERIETKNTHGTGCTLSSAIASNLAKGKDLFHAVSEAKEYVRNAIYY 249
Query: 246 GHRIGHGHCPVN 257
+G G P N
Sbjct: 250 SLNLGKGCGPTN 261
>gb|AAB72162.1| (AF000657) probable thiamin biosynthetic enzyme [Arabidopsis
thaliana]
Length = 525
Score = 183 bits (461), Expect = 2e-45
Identities = 101/251 (40%), Positives = 155/251 (61%), Gaps = 13/251 (5%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
+R V LT+AGSDSG GAGI+ADLK A GV+ IT+VTAQNT+ V ++H +PP+
Sbjct: 27 VRKVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEF 86
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYD---------FPLVVDPVMIAK 111
+SEQ+++V D D KTGML + EI++ + + + ++ F LVVDPVM++
Sbjct: 87 ISEQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRGRDYLALFSLVVDPVMVST 146
Query: 112 SGAPLLREDAVDALIKYILPLATLVTPNRFEAEK-LSGMKIRTVEDAKIAAKKIAEETGA 170
SG L + + +LP+A ++TPN EA L G +I TV + + AAK + E G
Sbjct: 147 SGHVLAGSSILSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSL-HEMGP 205
Query: 171 KAVIVKGGHI-EGEEAIDVLYHNGSYRLYRAPKV-EGCTHGTGCSFSAAITANLAKGFGL 228
+ V+VKGG + + +++DV + + R+P++ THGTGC+ ++ I A LAKG +
Sbjct: 206 RFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSM 265
Query: 229 EDAIEVAKKFI 239
A++VAK+F+
Sbjct: 266 LSAVKVAKRFV 276
>pir||T07834 hydroxymethylpyrimidine kinase (EC 2.7.1.49) / thiamin-phosphate
pyrophosphorylase (EC 2.5.1.3) - rape
>gi|2746079|gb|AAC31298.1| (AF015310) BTH1 [Brassica
napus]
Length = 523
Score = 182 bits (456), Expect = 1e-44
Identities = 100/243 (41%), Positives = 154/243 (63%), Gaps = 5/243 (2%)
Query: 1 MRLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDV 60
+R V LT+AGSDSG GAGI+AD+K A GV+ T+V A+NT+AV ++H +PPD
Sbjct: 29 VRKVAQVLTVAGSDSGAGAGIQADIKVCAARGVYCASVKTAVKAKNTRAVQSVHLLPPDS 88
Query: 61 VSEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLRE 119
VSEQ+++V D VD KTGML + EI++ + + + Y LVVDPVM++ SG L
Sbjct: 89 VSEQLKSVLSDFEVDVVKTGMLPSPEIVEVLLQNLSEYPVRALVVDPVMVSTSGHVLAGS 148
Query: 120 DAVDALIKYILPLATLVTPNRFEAEK-LSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGG 178
+ + +LPLA ++TPN EA L G++I+TV + + AAK + + G + V+VKGG
Sbjct: 149 SILSIFRERLLPLADIITPNVKEASALLGGVRIQTVAEMRSAAKSL-HQMGPRFVLVKGG 207
Query: 179 HI-EGEEAIDVLYHNGSYRLYRAPKV-EGCTHGTGCSFSAAITANLAKGFGLEDAIEVAK 236
+ + +++DV + + +P++ THGTGC+ ++ I A LAKG + A++VAK
Sbjct: 208 DLPDSSDSVDVYFDGNEFHELHSPRIATRNTHGTGCTLASCIAAELAKGSNMLSAVKVAK 267
Query: 237 KFI 239
+F+
Sbjct: 268 RFV 270
>pir||F75613 phosphomethylpyrimidine kinase - Deinococcus radiodurans (strain
R1) >gi|6460493|gb|AAF12199.1|AE001862_25 (AE001862)
phosphomethylpyrimidine kinase [Deinococcus radiodurans]
Length = 251
Score = 181 bits (455), Expect = 1e-44
Identities = 112/256 (43%), Positives = 144/256 (55%), Gaps = 19/256 (7%)
Query: 4 VKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSE 63
V ALTIAGSDSGGGAGI+ADLKTF A GV+G IT VTAQNT V + + P +V
Sbjct: 5 VPVALTIAGSDSGGGAGIQADLKTFEARGVYGTSVITLVTAQNTLGVQGVDYVSPYMVRA 64
Query: 64 QIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
Q+RAV +D V A KTG L EI++ VA+ ++ PLVVDPVM+AKSG LL +A+
Sbjct: 65 QLRAVLDDFPVGAVKTGALGRPEIVRVVAEELRGRGLPLVVDPVMLAKSGDALLDAEAIS 124
Query: 124 ALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIE-- 181
++ +LPLATLVTPN E L A +++KGGH E
Sbjct: 125 TVLGELLPLATLVTPNTPEWNVLCQ----------------AGAPDTLPLLLKGGHAEEG 168
Query: 182 GEEAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT 240
+ D L AP++ THGTGC+ SAAITANLA+G L A+ A ++
Sbjct: 169 SDSVTDELRTPAHSLTLTAPRLRTRHTHGTGCTLSAAITANLARGLDLPGAVMQAHAYLQ 228
Query: 241 LGIAMGHRIGHGHCPV 256
+ +G GH P+
Sbjct: 229 GALRRAPGLGAGHGPL 244
>sp|P76422|THID_ECOLI PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|7449911|pir||F64977 hypothetical protein
b2103 - Escherichia coli (strain K-12)
>gi|1736827|dbj|BAA15971.1| (D90848)
ORF_ID:o359#15~similar to [SwissProt Accession Number
P44697] [Escherichia coli] >gi|1788420|gb|AAC75164.1|
(AE000299) phosphomethylpyrimidine kinase [Escherichia
coli K12] >gi|4589382|dbj|BAA76742.1| (D84200)
phosphomethylpyrimidine kinase [Escherichia coli]
Length = 266
Score = 180 bits (452), Expect = 3e-44
Identities = 102/260 (39%), Positives = 154/260 (59%), Gaps = 5/260 (1%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIR 66
ALTIAG+D GGAGI+ADLKTF+A G +G IT++ AQNT+ V +++ I PD V+ Q+
Sbjct: 6 ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLD 65
Query: 67 AVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFP-LVVDPVMIAKSGAPLLREDAVDAL 125
+V D+ +D K GML+ +I++AVA+ ++ Y +V+D VM+AKSG PLL AV L
Sbjct: 66 SVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPSAVATL 125
Query: 126 IKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEA 185
+LP +L+TPN EA L E + + G AV++KGGH++ E++
Sbjct: 126 RSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRSLLAMGCGAVLMKGGHLDDEQS 185
Query: 186 IDVLYHNGSYRLYRAPKV-EGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIA 244
D L+ + + AP++ THGTGC+ SAA+ A + D ++ AK +++ +A
Sbjct: 186 PDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWLSSALA 245
Query: 245 MGH--RIGHGHCPVNQ-SAW 261
+GHG PV+ AW
Sbjct: 246 QADTLEVGHGIGPVHHFHAW 265
>pir||T47257 thiamin biosynthesis protein thi-4 [imported] - Neurospora crassa
(fragment) >gi|4432914|dbj|BAA21049.1| (D45894)
thiamine-4 [Neurospora crassa]
Length = 500
Score = 178 bits (447), Expect = 1e-43
Identities = 117/292 (40%), Positives = 169/292 (57%), Gaps = 37/292 (12%)
Query: 2 RLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVV 61
R + L IAGSDS GGAG+EAD K A G + + A T++TAQNT+ V IH++P D +
Sbjct: 9 RTLPRVLVIAGSDSSGGAGLEADQKVIAAHGCYAMTATTALTAQNTKGVYGIHEVPVDFL 68
Query: 62 SEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLRED 120
+QI AV D+GVD KTGML++A I+AVA+ ++++ LV+DPVMIA +GA LL
Sbjct: 69 RKQIDAVVGDVGVDVVKTGMLASAGTIEAVAQALQDHKLKTLVIDPVMIATTGAELLPNS 128
Query: 121 AVDALIKYILPLATLVTPNRFEAEKL------SGMKIRTVEDAKIAAKKIAEETGAKAVI 174
A AL + +LP+AT++TPN EA KL +++V D + A K+ ++ G+K V+
Sbjct: 129 ASRALCEKLLPIATILTPNVPEANKLLLETGHEERPVQSVVDLEDIAIKV-QKLGSKWVL 187
Query: 175 VKGGHI----EGEEA---------IDVLYHNGSYRLYR---------APKVE------GC 206
VKGGH +G EA ++VL S + R A ++E
Sbjct: 188 VKGGHTPFRRDGTEAKTEDEMEIVVNVLVGPASGKTKRDTEGEEKLQAVRIEMPYQRSRN 247
Query: 207 THGTGCSF-SAAITANLAKGFGLEDAIEVAKKFITLGIAMGHRIGHGHCPVN 257
THGTGCS SAAI +NLAKG + A++ +++ I +G G+ P+N
Sbjct: 248 THGTGCSLASAAIASNLAKGMDMVPAVKAGIRYVDAAIRTAPGLGQGNGPLN 299
>sp|P55882|THID_SALTY PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|1842118|gb|AAB66492.1| (U87940) HMP-P kinase
[Salmonella typhimurium]
Length = 266
Score = 171 bits (429), Expect = 1e-41
Identities = 99/260 (38%), Positives = 153/260 (58%), Gaps = 5/260 (1%)
Query: 7 ALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIR 66
ALTIAG+D GGAGI+ADLKTF+A G +G IT++ A+NT V +++ I PD V+ Q+
Sbjct: 6 ALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAENTCGVQSVYRIEPDFVAAQLD 65
Query: 67 AVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLREDAVDAL 125
+V D+ +D K GML+ +I++AVA+ ++ + +V+D VM+AKSG PLL A++ L
Sbjct: 66 SVFSDVRIDTTKIGMLAETDIVEAVAERLQRHHVRNVVLDTVMLAKSGDPLLSPSAIETL 125
Query: 126 IKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEA 185
+LP +L+TPN EA L E +A + G +AV++KGGH+E ++
Sbjct: 126 RVRLLPQVSLITPNLPEAAALLDAPHARTEQEMLAQGRALLAMGCEAVLMKGGHLEDAQS 185
Query: 186 IDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIA 244
D L+ + + AP+V THGTGC+ SAA+ A + + + AK +++ +A
Sbjct: 186 PDWLFTREGEQRFSAPRVNTKNTHGTGCTLSAALAALRPRHRSWGETVNEAKAWLSAALA 245
Query: 245 MGH--RIGHGHCPVNQ-SAW 261
+G G PV+ AW
Sbjct: 246 QADTLEVGKGIGPVHHFHAW 265
>sp|O83153|THID_TREPA PHOSPHOMETHYLPYRIMIDINE KINASE (HMP-PHOSPHATE KINASE) (HMP-P
KINASE) >gi|7449915|pir||B71365 probable
phosphomethypyrimidine kinase (thiD) - syphilis
spirochete >gi|3322378|gb|AAC65105.1| (AE001196)
phosphomethypyrimidine kinase (thiD) [Treponema
pallidum]
Length = 269
Score = 167 bits (418), Expect = 3e-40
Identities = 99/261 (37%), Positives = 149/261 (56%), Gaps = 8/261 (3%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTA--IHDIPPDVVSEQI 65
L+I GSD+ GGAGIEADLKTF +G G+ +T++ + + +H + D V +Q+
Sbjct: 5 LSIGGSDASGGAGIEADLKTFQEYGAFGVATLTAIVTMDPSRNWSHRVHSLEEDCVRDQL 64
Query: 66 RAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVV-DPVMIAKSGAPLLREDAVDA 124
+GV A K+GML++ I+ VA+ ++ + V DPVM+ K L + +
Sbjct: 65 ETAFAGVGVSAVKSGMLASVHAIECVAEYLERFAVAAYVFDPVMVCKGSGDALHRELNEL 124
Query: 125 LIKYILPLATLVTPNRFEAEKLSGMKI-RTVEDAKIAAKKIAEETGAKAVIVK-GGHIEG 182
+I+ +LP AT+VTPN FE +++G+ + RTV++ K A+ I E GA V VK GG + G
Sbjct: 125 MIQKLLPRATVVTPNLFETAQIAGISVPRTVDEMKEGARLI-HERGASHVFVKGGGRLPG 183
Query: 183 -EEAIDVLYHNGSYRLYRAPKVE-GCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFIT 240
+ A+DV Y ++ L V+ G HG GC+ SAAITA L +G DAI AK+F+T
Sbjct: 184 CKHALDVFYDGKTFHLVEDELVQSGWNHGAGCTVSAAITAGLGRGLTAYDAILSAKRFVT 243
Query: 241 LGIAMGHRIGHGHCPVNQSAW 261
G+ G ++ N S W
Sbjct: 244 TGLRHGFQVNQWVGTGNLSKW 264
>pir||T40812 probable thiamin biosynthesis protein - fission yeast
(Schizosaccharomyces pombe) >gi|3810842|emb|CAA21803.1|
(AL032684) putative thiamine biosynthesis protein
[Schizosaccharomyces pombe]
Length = 551
Score = 163 bits (409), Expect = 3e-39
Identities = 102/258 (39%), Positives = 145/258 (55%), Gaps = 21/258 (8%)
Query: 2 RLVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVV 61
R + +LTIAGSD GGAGI+ADLKT T+ GV+G+ AIT + A+N V ++ ++ P V
Sbjct: 33 RALPVSLTIAGSDCSGGAGIQADLKTMTSLGVYGMSAITCLVAENAGGVDSVEEMSPAFV 92
Query: 62 SEQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLRED 120
QI DI KTGML + EI+KAVA++ K ++F LVVDPVM+A SG L+ +D
Sbjct: 93 ESQIDCCIRDIPCHVVKTGMLGSPEIVKAVARSAKKFNFSKLVVDPVMVATSGDSLVTKD 152
Query: 121 AVDALIKYILPLATLVTPNRFEA------EKLSGMKIRTVEDAKIAAKKIAEETGAKAVI 174
V L + +LPL LVTPN EA + L I +V D + A + + G K V+
Sbjct: 153 IVSVLNEELLPLTYLVTPNIPEAIVLAKNQGLDISNINSVSDMERCA-AVIHKLGPKHVL 211
Query: 175 VKGGHI------------EGEEAIDVLYH-NGSYRLYRAPKVEGCTHGTGCSFSAAITAN 221
+KGGH+ E +D+LY N Y + +G HGTGC+ S+AI +
Sbjct: 212 LKGGHMPVNNLGLKSSDDEDLRVVDILYDGNRFYHFSSSYLKKGEVHGTGCTLSSAIASF 271
Query: 222 LAKGFGLEDAIEVAKKFI 239
LA L +A++ ++
Sbjct: 272 LAWEHSLTEAVQFGIDYV 289
>gi|6324997 Thi21p [Saccharomyces cerevisiae] >gi|2132251|pir||S65289
hypothetical protein YPL258c - yeast (Saccharomyces
cerevisiae) >gi|1370532|emb|CAA97986.1| (Z73614) ORF
YPL258c [Saccharomyces cerevisiae]
Length = 551
Score = 154 bits (386), Expect = 2e-36
Identities = 102/279 (36%), Positives = 152/279 (53%), Gaps = 16/279 (5%)
Query: 6 TALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQI 65
T L+IAG+D GGAG+EAD+KT TA + + IT++ AQ V +I++ P +VVS+ +
Sbjct: 25 TVLSIAGTDPSGGAGVEADVKTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVSQIL 84
Query: 66 RAVAEDIGVDAAKTGMLSNA--EIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
A +D+ D KTGML+ A E++ + LVVDPV++A SG+ L +D
Sbjct: 85 DANLQDMKCDVIKTGMLTTAAIEVLHEKLLQLGENRPKLVVDPVLVATSGSSLAGKDIAS 144
Query: 124 ALIKYILPLATLVTPNRFEAEKLSG--MKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI- 180
+ + I P A ++TPN E KL G +I + D AK +A+ T ++VKGGHI
Sbjct: 145 LITEKIAPFADILTPNIPECFKLLGEDREISKLRDIFEVAKDLAKITKCSNILVKGGHIP 204
Query: 181 ----EGEEAIDVLYHNGSYRL--YRAPKVEGC-THGTGCSFSAAITANLAKGFGLEDA-- 231
EG+ DVLY R ++ V THGTGC+ ++AI +NLA+G+ L +
Sbjct: 205 WNDEEGKYITDVLYLGAEQRFITFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVY 264
Query: 232 --IEVAKKFITLGIAMGHRIGHGHCPVNQSAWIEIPAEK 268
IE + + +G + + P+N IEIP EK
Sbjct: 265 GGIEYVQNAVAIGCDVTKETVKDNGPINHVYAIEIPLEK 303
>emb|CAA62531.1| (X91067) transcription factor [Saccharomyces cerevisiae]
Length = 543
Score = 147 bits (368), Expect = 2e-34
Identities = 98/279 (35%), Positives = 155/279 (55%), Gaps = 16/279 (5%)
Query: 6 TALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQI 65
T L+IAG+D GGAGIEAD+KT TA + + IT++ AQ V +I++ P +VV + +
Sbjct: 25 TVLSIAGTDPSGGAGIEADVKTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVFQTL 84
Query: 66 RAVAEDIGVDAAKTGMLSNA--EIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
+ +D+ + KTGML+ A E++ + LVVDPV++A SG+ L +D V
Sbjct: 85 ESNLKDMKCNVIKTGMLTAAAIEVLHEKLLQLGENRPKLVVDPVLVATSGSSLAGKDIVS 144
Query: 124 ALIKYILPLATLVTPNRFEAEKLSG--MKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI- 180
+ + + P A ++TPN E KL G K+ ++D AK +A+ T ++VKGGHI
Sbjct: 145 LITEKVAPFADILTPNIPECYKLLGEERKVNGLQDIFQIAKDLAKITKCSNILVKGGHIP 204
Query: 181 ---EGEEAI-DVLYHNGSYR--LYRAPKVEGC-THGTGCSFSAAITANLAKGFGLEDA-- 231
E E+ I DVL+ + +++ V THGTGC+ ++AI +NLA+G+ L +
Sbjct: 205 WNDEKEKYITDVLFLGAEQKFIIFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVY 264
Query: 232 --IEVAKKFITLGIAMGHRIGHGHCPVNQSAWIEIPAEK 268
IE + + +G + + P+N +EIP EK
Sbjct: 265 GGIEYVQNAVAIGCDVTKETVKDNGPINHVYAVEIPLEK 303
>gi|6324517 Thi20p [Saccharomyces cerevisiae] >gi|2133057|pir||S66740 probable
transcription factor YOL055c - yeast (Saccharomyces
cerevisiae) >gi|1419865|emb|CAA99063.1| (Z74797) ORF
YOL055c [Saccharomyces cerevisiae]
Length = 551
Score = 147 bits (368), Expect = 2e-34
Identities = 98/279 (35%), Positives = 155/279 (55%), Gaps = 16/279 (5%)
Query: 6 TALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQI 65
T L+IAG+D GGAGIEAD+KT TA + + IT++ AQ V +I++ P +VV + +
Sbjct: 25 TVLSIAGTDPSGGAGIEADVKTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVFQTL 84
Query: 66 RAVAEDIGVDAAKTGMLSNA--EIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
+ +D+ + KTGML+ A E++ + LVVDPV++A SG+ L +D V
Sbjct: 85 ESNLKDMKCNVIKTGMLTAAAIEVLHEKLLQLGENRPKLVVDPVLVATSGSSLAGKDIVS 144
Query: 124 ALIKYILPLATLVTPNRFEAEKLSG--MKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI- 180
+ + + P A ++TPN E KL G K+ ++D AK +A+ T ++VKGGHI
Sbjct: 145 LITEKVAPFADILTPNIPECYKLLGEERKVNGLQDIFQIAKDLAKITKCSNILVKGGHIP 204
Query: 181 ---EGEEAI-DVLYHNGSYR--LYRAPKVEGC-THGTGCSFSAAITANLAKGFGLEDA-- 231
E E+ I DVL+ + +++ V THGTGC+ ++AI +NLA+G+ L +
Sbjct: 205 WNDEKEKYITDVLFLGAEQKFIIFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVY 264
Query: 232 --IEVAKKFITLGIAMGHRIGHGHCPVNQSAWIEIPAEK 268
IE + + +G + + P+N +EIP EK
Sbjct: 265 GGIEYVQNAVAIGCDVTKETVKDNGPINHVYAVEIPLEK 303
>pir||T40811 probable thiamin biosynthesis protein - fission yeast
(Schizosaccharomyces pombe) >gi|3810841|emb|CAA21802.1|
(AL032684) putative thiamine biosynthesis protein
[Schizosaccharomyces pombe]
Length = 506
Score = 144 bits (359), Expect = 2e-33
Identities = 122/418 (29%), Positives = 197/418 (46%), Gaps = 49/418 (11%)
Query: 3 LVKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVS 62
++ T +T+AGSD GGAG++ADLK FTA V+G+ A+T++T+QNT V +H IP V
Sbjct: 1 MISTCITVAGSDCSGGAGVQADLKVFTAHSVYGMSAVTAITSQNTIGVNGVHLIPASYVE 60
Query: 63 EQIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLREDA 121
+QI A D+ + KTGML N +I+K + +++ + +VVDP++ + GA L+ D
Sbjct: 61 QQISACLLDVHCEVMKTGMLFNQQILKVIVESIDRFKIKKVVVDPLIATRKGALLVMPDY 120
Query: 122 VDALIKYILPLATLVTPNRFEAEKL------SGMKIRTVEDAKIAAKKIAEETGAKAVIV 175
++ +K ++P A ++ PN EA + ++I +ED K KK+ + G K V++
Sbjct: 121 LELFVKELIPRAEVLIPNIAEALIILKHMTNEFVEIHHLEDVKAVGKKLI-KAGCKNVVI 179
Query: 176 KGGHIEGEEAIDVLYHNG---SYRLYRAPKVEG------------CTHGTGCSFSAAITA 220
+ I ++ LY EG GT C+ S+A+ +
Sbjct: 180 RCDDIPFASDFFCSRETNMPPTWYLYVLCTSEGEVLLPQKWLASKTARGTSCALSSAVAS 239
Query: 221 NLAKGFGLEDAIEVAKKFITLGIAMGHRIGHGHCPVN-QSAWIEIPAEKWRIYEELTRAV 279
NLA G L A + A + + M +G G ++ SA +P EK E V
Sbjct: 240 NLAIGLDLVTATQNAVSYTQRALEMSFHLGRGANSLDYASALTRLPYEK----GEFINFV 295
Query: 280 REFEEMNPVKFIPEVGTNFVYSLPLPYARTRDDVAGVKGRIVR---VGNEVRAVGPVEFG 336
R + P K++ V FV L A T + K +++ + N +A G + F
Sbjct: 296 RYHPSITP-KWLSIVNHPFVEQLK---AGTLSRLPFQKYLVLKYHMLINNAQAAGMMAFS 351
Query: 337 ASDHLARAVLTFMKFYPEYRSAINIRYSEETV---RLAEEQGLVVSFYDRREEPEEIK 391
+S A E+ + I EE V R+ E+ GL S + +PE +K
Sbjct: 352 SSSISA----------IEHSAKIIQAIKEENVTHLRICEQYGLSAS-QITKSKPEIVK 398
>gi|6325378 Thi22p [Saccharomyces cerevisiae] >gi|2132289|pir||S69014
hypothetical protein YPR121w - yeast (Saccharomyces
cerevisiae) >gi|1066472|gb|AAB68062.1| (U40828) Weak
similarity to hypothetical protein 3 (A. thaliana, PIR
accession number S23315) [Saccharomyces cerevisiae]
Length = 572
Score = 141 bits (353), Expect = 1e-32
Identities = 93/278 (33%), Positives = 150/278 (53%), Gaps = 15/278 (5%)
Query: 6 TALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQI 65
T ++IAGSDS GGAG+EAD+KT TA + + +T++TAQ V H+IP +VS+ +
Sbjct: 47 TVMSIAGSDSSGGAGVEADIKTITAHRCYAMTCVTTLTAQTPVKVYGAHNIPKKMVSQIL 106
Query: 66 RAVAEDIGVDAAKTGMLS--NAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
A +D+ + KTGML+ E++ + LV+DPV+ A S + +D V
Sbjct: 107 DANLQDMKCNVIKTGMLTVDAIEVLHEKLLQLGENRPKLVIDPVLCAASDSSPTGKDVVS 166
Query: 124 ALIKYILPLATLVTPNRFEAEKLSG--MKIRTVEDAKIAAKKIAEETGAKAVIVKGGHI- 180
+I+ I P A ++TPN + L G ++ ++D AK ++ T ++VKGGHI
Sbjct: 167 LIIEKISPFADILTPNISDCFMLLGENREVSKLQDVLEIAKDLSRITNCSNILVKGGHIP 226
Query: 181 --EGEE--AIDVLYHNGSYRL--YRAPKVEGC-THGTGCSFSAAITANLAKGFGLEDAIE 233
+G+E DVLY + ++ V THG GC+ ++AI +NLA+G+ L ++
Sbjct: 227 CDDGKEKHITDVLYLGAEQKFITFKGQFVNTTRTHGAGCTLASAIASNLARGYSLSQSVY 286
Query: 234 VAKKFITLGIAMGHRIGHGHC---PVNQSAWIEIPAEK 268
+++ IA+G + P+N +EIP EK
Sbjct: 287 GGIEYVQNAIAIGCDVTKKAVKVGPINHVYAVEIPLEK 324
>gi|11499790 hydroxymethylpyrimidine phosphate kinase (thiD) [Archaeoglobus
fulgidus] >gi|7449912|pir||H69525
hydroxymethylpyrimidine phosphate kinase (thiD) homolog
- Archaeoglobus fulgidus >gi|2648317|gb|AAB89045.1|
(AE000953) hydroxymethylpyrimidine phosphate kinase
(thiD) [Archaeoglobus fulgidus]
Length = 243
Score = 136 bits (338), Expect = 7e-31
Identities = 85/247 (34%), Positives = 136/247 (54%), Gaps = 12/247 (4%)
Query: 4 VKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSE 63
+KT LTI+G D GGAG+ AD+KT A G++ ITS+T QNT V A + +V+ E
Sbjct: 1 MKTILTISGLDPSGGAGVSADIKTAKAIGLYPASVITSLTVQNTCGVKAAIPVEAEVIRE 60
Query: 64 QIRAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDFPLVVDPVMIAKSGAPLLREDAVD 123
Q+RAV ED+ +D K G++++ EI K +A + + P V+DPV+ A +G + E+A
Sbjct: 61 QLRAVIEDMKIDCIKIGLITSVEIAKVLAAELVPLNAPKVLDPVIFAGAGGKIGDEEAYK 120
Query: 124 ALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGE 183
++K++ +VTPN EA L + + ++A+ A ++ ++ G V++ GG + G+
Sbjct: 121 MILKHV----DIVTPNLTEAITL----LESTKEARELALEMNKKYGCD-VVITGGELGGK 171
Query: 184 EAIDVLYHNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGI 243
DV+ G A HGTGC +S A+ L G LE+A+ A+ F+ +
Sbjct: 172 ---DVVCEAGRTYTVSAEFSPVNIHGTGCVYSTALACYLGLGNSLENAVRKARIFVLESV 228
Query: 244 AMGHRIG 250
R G
Sbjct: 229 KKALRPG 235
>pir||A69215 conserved hypothetical protein MTH861 - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621953|gb|AAB85359.1| (AE000862) conserved protein
[Methanobacterium thermoautotrophicum]
Length = 190
Score = 129 bits (320), Expect = 8e-29
Identities = 64/150 (42%), Positives = 91/150 (60%), Gaps = 2/150 (1%)
Query: 291 IPEVGTNFVYSLPLPYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMK 350
IPEV +N V + P +DV V GRI A E+GAS H+AR ++ +
Sbjct: 26 IPEVRSNIVMARSNPCGP--EDVVAVPGRITEFQGRAFACRDPEYGASSHMARFIIALNE 83
Query: 351 FYPEYRSAINIRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVK 410
P RSA+NI++ E + + E+ GLVVS YDR EP+ +++ EG +IPWG+E A++ +
Sbjct: 84 HLPGRRSALNIKFDESIISICEDMGLVVSSYDRSREPDSVRDTEGGSIPWGVEEALRNSE 143
Query: 411 QRPDVIYHLGDVGKEPMILIFGRNPREVLD 440
PDVIYH G GKEPMI++ GR+ EV +
Sbjct: 144 SPPDVIYHRGAWGKEPMIVLTGRDAVEVAE 173
>gb|AAC27706.1| (AF061946) putative kinase Pak1p [Pichia angusta]
Length = 592
Score = 123 bits (306), Expect = 4e-27
Identities = 97/295 (32%), Positives = 151/295 (50%), Gaps = 33/295 (11%)
Query: 4 VKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSE 63
V L +AGSDS GGAGIEAD+KT TA+ + + IT++TAQN+ V + P +VV +
Sbjct: 32 VPRVLAVAGSDSSGGAGIEADVKTITAYKCYAVTCITALTAQNSLGVHNVTTTPAEVVEK 91
Query: 64 QIRAVAED-IGVDAAKTGMLSNAEIIKAVAK--TVKNYDFPLVVDPVMIAKSGAPLLRED 120
+ A+A+D I +DA K GML + E + + + P+V+DPV +AK+G L
Sbjct: 92 VLVAIADDQIALDAIKLGMLPD-ETLSVITPFLAARAARTPIVLDPVFVAKNGDRLSSVA 150
Query: 121 AVDALIKYILPLATLVTPNRFEAEKL-----------SGMKIRTVEDAKIAAKKIAEETG 169
A+ ++ + A L+TPN E E L + I T +D AA+ + +
Sbjct: 151 ALKKAVE-LFQHAALITPNSREVEVLLAVLRSSDPGVEEITISTADDFYTAARLLGSKFK 209
Query: 170 AKAVIVKGGHIEGEE-----------AIDVLYHNGSYRL--YRAPKVEGCT-HGTGCSFS 215
V+ KGG + + ++VLY + S +L +R+ ++ HGTGC+ S
Sbjct: 210 VD-VLFKGGLVPVNKNLVVDPENPVYILNVLYEHRSEKLTVFRSNHIDSPNLHGTGCTLS 268
Query: 216 AAITANLAKGFGLEDAIEVAKKFITLGIAMGHRIGHGHCPVNQSAWIEIPAEKWR 270
+A+ NLA+G L A+E A F+ I +G P+N + I+ P E R
Sbjct: 269 SAVACNLARGTSLATAVENAIGFVNEAIRHAEVKPNG--PLNHTWAIKRPHETSR 321
>pir||E83149 phosphomethylpyrimidine kinase PA3975 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9950165|gb|AAG07362.1|AE004815_6 (AE004815)
phosphomethylpyrimidine kinase [Pseudomonas aeruginosa]
Length = 265
Score = 123 bits (305), Expect = 5e-27
Identities = 77/247 (31%), Positives = 131/247 (52%), Gaps = 7/247 (2%)
Query: 8 LTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQIRA 67
L ++G D GGAG++AD++ A G H A+T++T Q+T V+ + + V Q A
Sbjct: 11 LCLSGHDPSGGAGLQADIEALLAQGCHAAPAVTALTVQDTVNVSDFRVLDREWVLAQANA 70
Query: 68 VAEDIGVDAAKTGMLSNAEIIKAVAKTVKNY-DFPLVVDPVMIAKSGAPLLREDAVDALI 126
V D+ V A K GML + E++ VA+ + + P+V DPV+ A G L + D A+
Sbjct: 71 VLADLPVAAVKLGMLGSLEMVDTVAEILAAHPHLPVVCDPVLRAGGGGALGKGDVGFAMR 130
Query: 127 KYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKGGHIEGEEAI 186
+ +LP AT+ TPN EA L+ + + + A+++ E + +++ GGH + E
Sbjct: 131 ERLLPRATIATPNLPEARLLADLPEGSADQ---CAERLLEH--CEHLLITGGHGDESEVH 185
Query: 187 DVLY-HNGSYRLYRAPKVEGCTHGTGCSFSAAITANLAKGFGLEDAIEVAKKFITLGIAM 245
+ LY +GS R + ++ G HG+GC+ ++A+ +A G L A++ A + +
Sbjct: 186 NRLYSRDGSRRTFTCQRLPGSYHGSGCTLASALAGRIALGEALHGAVQSALDYTWRTLRD 245
Query: 246 GHRIGHG 252
GHG
Sbjct: 246 AESPGHG 252
>pir||S14885 hypothetical protein 8 - yeast (Pichia angusta)
>gi|2770|emb|CAA41672.1| (X58862) ORF8 [Pichia angusta]
Length = 379
Score = 120 bits (298), Expect = 3e-26
Identities = 96/292 (32%), Positives = 147/292 (49%), Gaps = 33/292 (11%)
Query: 4 VKTALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSE 63
V LT+AGSDS GGAGIEAD+KT TA + + IT++TAQN+ V + P + V +
Sbjct: 32 VPRVLTVAGSDSSGGAGIEADVKTITAHKCYAVTCITALTAQNSLGVHNVTTTPAETVEK 91
Query: 64 QIRAVAED-IGVDAAKTGMLSNAEIIKAVAK--TVKNYDFPLVVDPVMIAKSGAPLLRED 120
+ A+A+D I +DA K GML + E + + + P+V+DPV +AK+G L
Sbjct: 92 VLVAIADDQIALDAIKLGMLPD-ETLSVITPFLAARAARTPIVLDPVFVAKNGDRLSSVG 150
Query: 121 AVDALIKYILPLATLVTPNRFEAEKL-----------SGMKIRTVEDAKIAAKKIAEETG 169
A+ ++ + A L+TPN E E L + I T +D AA+ +
Sbjct: 151 ALKKAVE-LFQHAALITPNSREVEVLLAVLRSSDPSVEEITITTADDFYTAARLLGSRFK 209
Query: 170 AKAVIVKGGHIEGEE-----------AIDVLYHNGSYRL--YRAPKVEGCT-HGTGCSFS 215
V+ KGG + E ++VLY + S +L +R+ ++ HGTGC+ S
Sbjct: 210 VD-VLFKGGLVPVNENLAVDPENPVYILNVLYEHRSEKLTVFRSNHIDSPNLHGTGCTLS 268
Query: 216 AAITANLAKGFGLEDAIEVAKKFITLGIAMGHRIGHGHCPVNQSAWIEIPAE 267
+A+ LA+G L A+E A F+ I +G P+N + I+ P E
Sbjct: 269 SAVACYLARGASLATAVENAIGFVNEAIRHAEVKPNG--PLNHTWAIKRPHE 318
>pir||S39961 Thi protein - Rhizobium meliloti >gi|742332|prf||2009367G Thi
protein [Rhizobium meliloti]
Length = 164
Score = 118 bits (292), Expect = 2e-25
Identities = 70/156 (44%), Positives = 93/156 (58%), Gaps = 3/156 (1%)
Query: 105 DPVMIAKSGAPLLREDAVDALIKYILPLATLVTPNRFEAEKLSGMKIRTVEDAKIAAKKI 164
DPVM+A SG LLR DAV ALI+ +LPLA +VTPN EA ++G I E +
Sbjct: 1 DPVMVATSGDALLRPDAVAALIEELLPLALVVTPNLAEAALMTGRAIAGDEAEMARQAEA 60
Query: 165 AEETGAKAVIVKGGHIEGEEAIDVLYHNGSYRLYRAPKVEGCT-HGTGCSFSAAITANLA 223
TGA AV+VKGGH++G+EA D+ + + A ++E HGTGC+ SAAI A LA
Sbjct: 61 IMRTGAHAVLVKGGHLKGQEATDLFFDGDTLVRLPAGRIETRNDHGTGCTLSAAIAAGLA 120
Query: 224 KGFGLEDAIEVAKKFITLGIAMGHR--IGHGHCPVN 257
KG L +A+ AK ++ I+ R IG G PV+
Sbjct: 121 KGVPLIEAVSAAKAYLHAAISAADRLEIGQGRGPVH 156
>gb|AAD39740.1| (AF105331) phosphomethylpyrimidine kinase [Clostridium
cellulovorans]
Length = 169
Score = 117 bits (290), Expect = 3e-25
Identities = 73/156 (46%), Positives = 103/156 (65%), Gaps = 9/156 (5%)
Query: 109 IAKSGAPLLREDAVDALIKYILPLATLVTPNRFEAEKL-----SGMK-IRTVEDAKIAAK 162
I+KSG LL+ +A ALI ++PLAT++TPN EAE++ S +K I+TVE+ AAK
Sbjct: 1 ISKSGYSLLKPEAKTALITKLVPLATVITPNLPEAEEILKEVNSNIKEIKTVEEMDKAAK 60
Query: 163 KIAEETGAKAVIVKGGHIEGEEAIDVLYHNGSYRLYRAPKVEGC-THGTGCSFSAAITAN 221
+I + G K V +KGGH+EGE AIDVLY + + +++ THGTGC+ S+AI AN
Sbjct: 61 EIYK-LGCKNVFLKGGHLEGE-AIDVLYDGKELTHFYSKRIQTKNTHGTGCTISSAIAAN 118
Query: 222 LAKGFGLEDAIEVAKKFITLGIAMGHRIGHGHCPVN 257
LA G+ ++DAI+ AK++IT+ I IG G P N
Sbjct: 119 LALGYSVKDAIQKAKEYITIAIKHSLDIGKGVGPTN 154
>pir||T41198 probable thiamin biosynthesis protein - fission yeast
(Schizosaccharomyces pombe) >gi|5701960|emb|CAB52152.1|
(AL109736) putative thiamine biosynthesis protein.
[Schizosaccharomyces pombe]
Length = 327
Score = 111 bits (274), Expect = 2e-23
Identities = 77/265 (29%), Positives = 123/265 (46%), Gaps = 23/265 (8%)
Query: 6 TALTIAGSDSGGGAGIEADLKTFTAFGVHGLVAITSVTAQNTQAVTAIHDIPPDVVSEQI 65
T LTIAGSD G AGI+ADLK TA V+G+ +T++T QN+ +T I+ + P ++ QI
Sbjct: 15 TCLTIAGSDCSGAAGIQADLKVMTAHQVYGMSVLTALTCQNSHGITGIYPLHPSLIQRQI 74
Query: 66 RAVAEDIGVDAAKTGMLSNAEIIKAVAKTVKNYDF-PLVVDPVMIAKSGAPLLREDAVDA 124
A DI K GML + + I +++ + Y +V+D V+I+ G + + A
Sbjct: 75 DACLSDIQCRVVKIGMLPDPKSIPVISQALTKYKITDVVMDSVIISSMGNVMCETPTIPA 134
Query: 125 LIKYILPLATLVTPNRFEA-------EKLSGMKIRTVEDAKIAAKKIAEETGAKAVIVKG 177
I+++ P + N EA K S +++ D + I G K V+++G
Sbjct: 135 TIQHLFPHLLVYASNVMEAFILVEKTLKKSPPPLKSFPDIQ-NLMSIIHRLGPKFVVLRG 193
Query: 178 GHIEGEE-------------AIDVLYHNGSYRLYRAP-KVEGCTHGTGCSFSAAITANLA 223
H+ ++ D++Y + ++ P HG CS +AAI +NLA
Sbjct: 194 HHVAFDKNMMITEKPDSKSWTADLIYDGKEFYIFEKPYNTTKSIHGESCSLTAAIASNLA 253
Query: 224 KGFGLEDAIEVAKKFITLGIAMGHR 248
AI A I I H+
Sbjct: 254 CNIPPLQAIHEALYSIEWAIQRVHK 278
>gb|AAK40404.1| Conserved hypothetical protein [Sulfolobus solfataricus]
Length = 190
Score = 106 bits (263), Expect = 4e-22
Identities = 62/156 (39%), Positives = 91/156 (57%), Gaps = 4/156 (2%)
Query: 291 IPEVGTNFVYSLPLPYARTRDDVAGVKGRIVRVGNEVRAVGPVEFGASDHLARAVLTFMK 350
IPEV TN Y++ A +DVA + GR+ +V FGASDH+AR +LT MK
Sbjct: 31 IPEVRTNIGYAVS--NAADANDVAAIPGRLTTAFKKVIYCMLPAFGASDHVARVILTVMK 88
Query: 351 FYPEYRSAINIRYSEETVRLAEEQGLVVSFYDRREEPEEIKEREGATIPWGIESAIKRVK 410
RSAIN++Y E + Q L + +DR EP+E+K RE +T+ + ++S I+++
Sbjct: 89 HDRNIRSAINLKYYREVIEKLPSQDLCI--FDRSSEPKEVKYREHSTMNFMVDSCIRKLS 146
Query: 411 QRPDVIYHLGDVGKEPMILIFGRNPREVLDKLRLLL 446
+ P+ I LGD GKEP + I R+P V++K LL
Sbjct: 147 KVPNYIVDLGDYGKEPSLFILDRDPVTVVNKSLELL 182
>pir||E71118 hypothetical protein PH0716 - Pyrococcus horikoshii
>gi|3257124|dbj|BAA29807.1| (AP000003) 296aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 296
Score = 94.4 bits (231), Expect = 2e-18
Identities = 61/181 (33%), Positives = 101/181 (55%), Gaps = 13/181 (7%)
Query: 266 AEKWRIYEELTRAVREF-EEMNPVKFIPEVGTNFVYSLPLPYARTRDDVAGVKGRIVRVG 324
+E+ + +ELT+A+ ++ + IPE+ +NF YS+ P R +DVA V GRI +
Sbjct: 124 SERKEVIDELTKALEILLKDEKFARLIPEIRSNFAYSISNP--RDLNDVAAVPGRITVIK 181
Query: 325 NEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAINIRYSEETVRLAEEQGLVVSFYDRR 384
++ A+ P EFGAS H A+ ++ ++ P+ RS INIR+ ++ ++ GL V + R
Sbjct: 182 DKPYAMPP-EFGASKHTAKLLIKISRYNPKIRSVINIRFGKDVEEAVKKAGLRVIYLPRN 240
Query: 385 EEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLGDVGKEPMILIFGRNPREVLDKLRL 444
+ E E E A I +G+ PD++ G G EP + +FG NP +V+ KLR+
Sbjct: 241 LKSIEEVENEIAKI-FGM--------GEPDIVIDPGRHGVEPCVYVFGENPWDVIRKLRV 291
Query: 445 L 445
+
Sbjct: 292 V 292
>pir||H75042 hypothetical protein PAB0877 - Pyrococcus abyssi (strain Orsay)
>gi|5458750|emb|CAB50237.1| (AJ248287) hypothetical
protein [Pyrococcus abyssi]
Length = 296
Score = 87.0 bits (212), Expect = 4e-16
Identities = 64/184 (34%), Positives = 98/184 (52%), Gaps = 15/184 (8%)
Query: 262 IEIPAEKWRIYEELTRAVREF-EEMNPVKFIPEVGTNFVYSLPLPYARTRDDVAGVKGRI 320
+ I + K + E+L+RA+ + + IPE+ +NF YSLP P R +DVA + GRI
Sbjct: 120 LAIESGKKEVIEDLSRALEIILRDEKFAELIPEIRSNFAYSLPNP--RDTNDVAAIPGRI 177
Query: 321 VRVGNEVRAVGPVEFGASDHLARAVLTFMKFYPEYRSAINIRYSEETVRLAEEQGLVVSF 380
+ + A+ P EFG S H AR ++ K + RS +NIR+ ++ ++ GL V++
Sbjct: 178 TVIKGKPYAMPP-EFGVSKHTARLLVKVSKHNKDIRSVLNIRFGKDVEDAIKKAGLRVAY 236
Query: 381 YDRR-EEPEEIKEREGATIPWGIESAIKRVKQRPDVIYHLGDVGKEPMILIFGRNPREVL 439
R EEI++R A + G E DV+ G G EP + IFGRNP +V+
Sbjct: 237 LPRELSSIEEIEDRI-AEMFNGNEF---------DVVIDPGRHGVEPCVYIFGRNPMDVI 286
Query: 440 DKLR 443
KL+
Sbjct: 287 VKLK 290
>sp|P40191|PDXK_ECOLI PYRIDOXINE KINASE (PYRIDOXAL KINASE) (VITAMIN B6 KINASE)
(PYRIDOXAMINE KINASE) (PN/PL/PM KINASE)
>gi|7466351|pir||A65016 hypothetical protein b2418 -
Escherichia coli (strain K-12)
>gi|1314674|gb|AAC44166.1| (U53700)
pyridoxine/pyridoxal/pyridoxamine kinase [Escherichia
coli] >gi|1788758|gb|AAC75471.1| (AE000329)
pyridoxal/pyridoxine/pyridoxamine kinase [Escherichia
coli K12] >gi|1799837|dbj|BAA16292.1| (D90871) similar
to [SwissProt Accession Number P40191] [Escherichia
coli]
Length = 283
Score = 73.7 bits (178), Expect = 4e-12
Identities = 64/209 (30%), Positives = 98/209 (46%), Gaps = 11/209 (5%)
Query: 38 AITSVTAQNTQAVTAIHD--IPPDVVSEQIRAVAEDIGVD---AAKTGMLSNAEIIKAVA 92
A+ +V NT + IP + S +RA+ E + A TG + A IK +A
Sbjct: 48 AVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQIKILA 107
Query: 93 K--TVKNYDFP---LVVDPVMIAKSGAPLLREDAVDALIKYILPLATLVTPNRFEAEKLS 147
+ T D P ++VDPV+ ++ D +A +Y+LPLA +TPN FE E L+
Sbjct: 108 EWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIFELEILT 167
Query: 148 GMKIRTVEDAKIAAKKIAEETGAKAVIVK-GGHIEGEEAIDVLYHNGSYRLYRAPKVEGC 206
G R ++ A AAK + +T V+ G+ E +E V+ S + +V+
Sbjct: 168 GKNCRDLDSAIAAAKSLLSDTLKWVVVTSASGNEENQEMQVVVVTADSVNVISHSRVKTD 227
Query: 207 THGTGCSFSAAITANLAKGFGLEDAIEVA 235
GTG F A + + L KG L DA+ A
Sbjct: 228 LKGTGDLFCAQLISGLLKGKALTDAVHRA 256
>gb|AAK40790.1| Conserved hypothetical protein [Sulfolobus solfataricus]
Length = 314
Score = 71.4 bits (172), Expect = 2e-11
Identities = 50/170 (29%), Positives = 84/170 (49%), Gaps = 11/170 (6%)
Query: 273 EELTRAVREFEEMNPVKFIPEVGTNFVYSLPLPYARTRDDVAGVKGRIVRVGNEVRAVGP 332
E + A+ + + + IPEV +N ++ A+ +DV + GRI ++ N
Sbjct: 143 EMMELALSMLQNESVAELIPEVLSNLAFAKA--NAKREEDVLAIPGRITKIRNLPTPASK 200
Query: 333 VEFGASDHLARAVLTFMKFYPEYRSAINIRYSEETVRLAEEQGLVVSFYDRREEPEEIKE 392
+G S HLA+ +L+ MK +P RS +NI+Y E+ + +E G F + + E
Sbjct: 201 PMWGGSKHLAKVLLSVMKNHPAVRSVMNIKYDEKVEMILKEIG--YKFKKVGPQNDLGNE 258
Query: 393 REGATIPWGIESAIKRVKQRPDVIYHLGDVGKEPMILIFGRNPREVLDKL 442
R TI +A+ + DV+ HLG G E +FGR+P +V+ K+
Sbjct: 259 RIAETI-----AAV--FDEGTDVVIHLGGTGIEANTYVFGRDPLDVVKKI 301
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.137 0.391
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 156421527
Number of Sequences: 2977
Number of extensions: 6403832
Number of successful extensions: 16750
Number of sequences better than 1.0e-10: 55
Number of HSP's better than 0.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 16568
Number of HSP's gapped (non-prelim): 56
length of query: 446
length of database: 189,106,746
effective HSP length: 58
effective length of query: 388
effective length of database: 154,394,500
effective search space: 59905066000
effective search space used: 59905066000
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)