BLASTP 2.0.10 [Aug-26-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= PAB1655 (PAB1655) DE:Nter region of INITIATION FACTOR EIF-2B
ALPHA SUBUNIT 1 from AQUIFEX AEOLICUS.
         (127 letters)

Database: ./suso.pep; /banques/blast2/nr.pep
           598,487 sequences; 189,106,746 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||A75086 translation initiation factor eIF-2b alpha chain 1 t...   256  1e-67
pir||B71055 hypothetical protein PH1136 - Pyrococcus horikoshii ...   225  2e-58
sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2...   140  5e-33

>pir||A75086 translation initiation factor eIF-2b alpha chain 1 truncated
           homolog PAB1655 [imported] - Pyrococcus abyssi (strain
           Orsay) >gi|5458502|emb|CAB49990.1| (AJ248286) Nter
           region of INITIATION FACTOR EIF-2B ALPHA SUBUNIT 1 from
           AQUIFEX AEOLICUS. [Pyrococcus abyssi]
           Length = 127
           
 Score =  256 bits (646), Expect = 1e-67
 Identities = 127/127 (100%), Positives = 127/127 (100%)

Query: 1   MEQRTHKLTSERLVGRPIKIEEGYAEVELVTIDEMKVDEKGLVHGGFTFGLADYAAMLAV 60
           MEQRTHKLTSERLVGRPIKIEEGYAEVELVTIDEMKVDEKGLVHGGFTFGLADYAAMLAV
Sbjct: 1   MEQRTHKLTSERLVGRPIKIEEGYAEVELVTIDEMKVDEKGLVHGGFTFGLADYAAMLAV 60

Query: 61  NEPTVVLGKAEVRFTKPVKVGDKLVAKAKIIDDQGRKKTVDVKVYRGEDVVLEGKFHCYV 120
           NEPTVVLGKAEVRFTKPVKVGDKLVAKAKIIDDQGRKKTVDVKVYRGEDVVLEGKFHCYV
Sbjct: 61  NEPTVVLGKAEVRFTKPVKVGDKLVAKAKIIDDQGRKKTVDVKVYRGEDVVLEGKFHCYV 120

Query: 121 LEKHVLD 127
           LEKHVLD
Sbjct: 121 LEKHVLD 127


>pir||B71055 hypothetical protein PH1136 - Pyrococcus horikoshii
           >gi|3257553|dbj|BAA30236.1| (AP000005) 131aa long
           hypothetical protein [Pyrococcus horikoshii]
           Length = 131
           
 Score =  225 bits (567), Expect = 2e-58
 Identities = 112/127 (88%), Positives = 120/127 (94%)

Query: 1   MEQRTHKLTSERLVGRPIKIEEGYAEVELVTIDEMKVDEKGLVHGGFTFGLADYAAMLAV 60
           +EQRTHKLTS  LVG+PI I+EGYAEVEL TIDEMKVDEKGLVHGGFTFGLADYAAMLAV
Sbjct: 4   VEQRTHKLTSRILVGKPILIKEGYAEVELETIDEMKVDEKGLVHGGFTFGLADYAAMLAV 63

Query: 61  NEPTVVLGKAEVRFTKPVKVGDKLVAKAKIIDDQGRKKTVDVKVYRGEDVVLEGKFHCYV 120
           NEPTVVLGKAEVRFTKPVKVGDKLVAKAKII+D G+KK V+VKVYR E+VVLEGKF+CYV
Sbjct: 64  NEPTVVLGKAEVRFTKPVKVGDKLVAKAKIIEDLGKKKIVEVKVYREEEVVLEGKFYCYV 123

Query: 121 LEKHVLD 127
           LEKHVLD
Sbjct: 124 LEKHVLD 130


>sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
           EXCHANGE FACTOR) >gi|7519844|pir||C70481 translation
           initiation factor eIF-2B alpha chain - Aquifex aeolicus
           >gi|2984318|gb|AAC07840.1| (AE000773) initiation factor
           eIF-2B alpha subunit [Aquifex aeolicus]
           Length = 456
           
 Score =  140 bits (350), Expect = 5e-33
 Identities = 66/116 (56%), Positives = 88/116 (74%)

Query: 1   MEQRTHKLTSERLVGRPIKIEEGYAEVELVTIDEMKVDEKGLVHGGFTFGLADYAAMLAV 60
           M+ RTH   ++ L G+P+K+ EGYAEV L T + MK D+KGLVHGGF F  ADYA+MLAV
Sbjct: 1   MDIRTHLGINQELSGKPVKVAEGYAEVLLKTQESMKADDKGLVHGGFIFSAADYASMLAV 60

Query: 61  NEPTVVLGKAEVRFTKPVKVGDKLVAKAKIIDDQGRKKTVDVKVYRGEDVVLEGKF 116
           N P VVL  A V+F KPVKVGD+++ KA++ +D+G+KK V V+ ++GED V EG+F
Sbjct: 61  NHPNVVLAGANVKFLKPVKVGDEIICKARVSEDKGKKKKVLVECFKGEDKVFEGEF 116


  Database: ./suso.pep
    Posted date:  Jul 6, 2001  5:57 PM
  Number of letters in database: 840,471
  Number of sequences in database:  2977
  
  Database: /banques/blast2/nr.pep
    Posted date:  Dec 14, 2000 12:46 PM
  Number of letters in database: 188,266,275
  Number of sequences in database:  595,510
  
Lambda     K      H
   0.318    0.139    0.387 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44620256
Number of Sequences: 2977
Number of extensions: 1684985
Number of successful extensions: 3924
Number of sequences better than 1.0e-10: 3
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3921
Number of HSP's gapped (non-prelim): 3
length of query: 127
length of database: 189,106,746
effective HSP length: 54
effective length of query: 73
effective length of database: 156,788,448
effective search space: 11445556704
effective search space used: 11445556704
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 160 (66.7 bits)