BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1658 (PAB1658) DE:TRANSMEMBRANE TRANSPORT PROTEIN (470 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75085 transmembrane transport protein PAB1658 - Pyrococcus... 945 0.0 pir||F71055 hypothetical protein PH1140 - Pyrococcus horikoshii ... 599 e-170 pir||D75104 transmembrane transport protein PAB1564 - Pyrococcus... 257 1e-67 pir||G71055 hypothetical protein PH1141 - Pyrococcus horikoshii ... 236 5e-61 pir||C69078 conserved hypothetical protein MTH1583 - Methanobact... 71 3e-11 >pir||E75085 transmembrane transport protein PAB1658 - Pyrococcus abyssi (strain Orsay) >gi|5458498|emb|CAB49986.1| (AJ248286) TRANSMEMBRANE TRANSPORT PROTEIN [Pyrococcus abyssi] Length = 470 Score = 945 bits (2416), Expect = 0.0 Identities = 470/470 (100%), Positives = 470/470 (100%) Query: 1 MKNKRALFLIISWALIFLPYVLTRKSPPGYIIGGDTLVHAAIARGIYLGRNPFLDQTYNV 60 MKNKRALFLIISWALIFLPYVLTRKSPPGYIIGGDTLVHAAIARGIYLGRNPFLDQTYNV Sbjct: 1 MKNKRALFLIISWALIFLPYVLTRKSPPGYIIGGDTLVHAAIARGIYLGRNPFLDQTYNV 60 Query: 61 PPNWYPFLYHLIVAIFAKIVGIEKSMLMLQAVFSLLMLLIMYWIAKNLWGETAGIFVLSL 120 PPNWYPFLYHLIVAIFAKIVGIEKSMLMLQAVFSLLMLLIMYWIAKNLWGETAGIFVLSL Sbjct: 61 PPNWYPFLYHLIVAIFAKIVGIEKSMLMLQAVFSLLMLLIMYWIAKNLWGETAGIFVLSL 120 Query: 121 SLLLLPAHRYPNPKELAPILGMISLYFSLRRKYYLGGLFAGLSLWTHYAVAAPLITFFLV 180 SLLLLPAHRYPNPKELAPILGMISLYFSLRRKYYLGGLFAGLSLWTHYAVAAPLITFFLV Sbjct: 121 SLLLLPAHRYPNPKELAPILGMISLYFSLRRKYYLGGLFAGLSLWTHYAVAAPLITFFLV 180 Query: 181 LAIKNRKYMISLALALLTFSPFIVNVILHAQFLLPHVEDIYRFWITDTLERKLSSLLPPL 240 LAIKNRKYMISLALALLTFSPFIVNVILHAQFLLPHVEDIYRFWITDTLERKLSSLLPPL Sbjct: 181 LAIKNRKYMISLALALLTFSPFIVNVILHAQFLLPHVEDIYRFWITDTLERKLSSLLPPL 240 Query: 241 YLIPLIFLALLRMYKEKDENAMKLFLFISIIAGMRLFPEILKPFGIDLWSSRFIGLLPYS 300 YLIPLIFLALLRMYKEKDENAMKLFLFISIIAGMRLFPEILKPFGIDLWSSRFIGLLPYS Sbjct: 241 YLIPLIFLALLRMYKEKDENAMKLFLFISIIAGMRLFPEILKPFGIDLWSSRFIGLLPYS 300 Query: 301 YVLLASYGVRSLNGEILNVMALTLIPVFASLNFWYSVINDPFIQASSVELNEYFPQEHFV 360 YVLLASYGVRSLNGEILNVMALTLIPVFASLNFWYSVINDPFIQASSVELNEYFPQEHFV Sbjct: 301 YVLLASYGVRSLNGEILNVMALTLIPVFASLNFWYSVINDPFIQASSVELNEYFPQEHFV 360 Query: 361 DVAEWIVKNTARDDVIGAGEEMGMMINALTGRPIIATMYGHGNIFLNNTERRHELKLLFL 420 DVAEWIVKNTARDDVIGAGEEMGMMINALTGRPIIATMYGHGNIFLNNTERRHELKLLFL Sbjct: 361 DVAEWIVKNTARDDVIGAGEEMGMMINALTGRPIIATMYGHGNIFLNNTERRHELKLLFL 420 Query: 421 GNCSEKIIVLQKYRVKYVIVDPLTEAKNMSCVGKVIFRKGDVEIVKVGEL 470 GNCSEKIIVLQKYRVKYVIVDPLTEAKNMSCVGKVIFRKGDVEIVKVGEL Sbjct: 421 GNCSEKIIVLQKYRVKYVIVDPLTEAKNMSCVGKVIFRKGDVEIVKVGEL 470 >pir||F71055 hypothetical protein PH1140 - Pyrococcus horikoshii >gi|3257557|dbj|BAA30240.1| (AP000005) 507aa long hypothetical protein [Pyrococcus horikoshii] Length = 507 Score = 599 bits (1528), Expect = e-170 Identities = 281/470 (59%), Positives = 367/470 (77%), Gaps = 4/470 (0%) Query: 1 MKNKRALFLIISWALIFLPYVLTRKSPPGYIIGGDTLVHAAIARGIYLGRNPFLDQTYNV 60 M+ F I W L F+PY++TRKSPPGYIIGGDTLVHAAIARGI LGRNPFLDQTYNV Sbjct: 1 MRKTYYFFFISIWTLTFIPYIITRKSPPGYIIGGDTLVHAAIARGILLGRNPFLDQTYNV 60 Query: 61 PPNWYPFLYHLIVAIFAK--IVGIEKSMLMLQAVFSLLMLLIMYWIAKNLWGETAGIFVL 118 PPNWYPFLYH+IVA AK + IEKSML+LQ + SLLM+L+M +A LWGE A I + Sbjct: 61 PPNWYPFLYHMIVAGVAKGFSISIEKSMLVLQLILSLLMILVMSLVAGKLWGEEAEIVSM 120 Query: 119 SLSLLLLPAHRYPNPKELAPILGMISLYFSLRRKYYLGGLFAGLSLWTHYAVAAPLITFF 178 +LSLL+LP HRYPNPKELAPI G+ISL++ RR+Y + G+ GL+LWTHYAVA PL+ FF Sbjct: 121 ALSLLMLPYHRYPNPKELAPIFGIISLFYLSRRRYIMSGILVGLALWTHYAVAFPLLLFF 180 Query: 179 LVLAIKNRKYMISLALALLTFSPFIVNVILHAQFLLPHVEDIYRFWITDTLERKLSSLLP 238 L L +K+R+Y++SL ++L+ FSPF+VN++LH++FL P VEDIY FW+ DT++RKL S+LP Sbjct: 181 LTLGLKDRRYILSLIISLIIFSPFVVNILLHSEFLFPQVEDIYAFWVNDTVKRKLLSVLP 240 Query: 239 PLYLIPLIFLALLRMYKEKDENAMKLFLFISIIAGMRLFPEILKPFGIDLWSSRFIGLLP 298 P+YL PL+ LA ++ YK ++ + L F+ +I RL PE LKPFGI LWSSRF LLP Sbjct: 241 PVYLAPLVALAGIKGYKRREREVLLLIAFVFVIIIARLSPETLKPFGIKLWSSRFTYLLP 300 Query: 299 YSYVLLASYGVRSL-NGEILNVMALTLIPVFASLNFWYSVINDPFIQASSVELNEYFPQE 357 Y YVLL++YG+ + ++ + LIP+ A +NF S+ DPF++ S+++L EYFPQE Sbjct: 301 YVYVLLSAYGMLNFAKSKLGKFILFFLIPILAVVNFSASIAEDPFLKVSTLDLKEYFPQE 360 Query: 358 HFVDVAEWIVKNTARDDVIGAGEEMGMMINALTGRPIIATMYGHGNIFLNNTERRHELKL 417 HFV+VAEW+ KN RDD I AGEEM MMINALTGRP++ATMYGHGN FLNNT RR +L++ Sbjct: 361 HFVEVAEWVKKNIERDDTIAAGEEMSMMINALTGRPVVATMYGHGNTFLNNTRRRKDLEI 420 Query: 418 LFLGNCSEKIIVLQKYRVKYVIVDPLTEAKNMSCVGKVIFRKGDVEIVKV 467 LFLGNC+EK +++KY+V+Y+I+DP T AK+M C+G ++++ G+V I+KV Sbjct: 421 LFLGNCNEKKGIVRKYKVRYIILDPPTTAKDMECLG-IVYKIGNVSILKV 469 >pir||D75104 transmembrane transport protein PAB1564 - Pyrococcus abyssi (strain Orsay) >gi|5458649|emb|CAB50137.1| (AJ248286) TRANSMEMBRANE TRANSPORT PROTEIN [Pyrococcus abyssi] Length = 503 Score = 257 bits (651), Expect = 1e-67 Identities = 174/473 (36%), Positives = 256/473 (53%), Gaps = 47/473 (9%) Query: 7 LFLIISWALIFLPYVLTRKSPPGYIIGGDTLVHAAIARGIYLGRNPFLDQTYNVPPNWYP 66 L+L++ ++ + RK P G GGDT++H A+ RGIYLGRNPFLDQ YNVPPNWYP Sbjct: 9 LWLLLIAFIVNFAVIYGRKMPVG-ANGGDTIIHMAMIRGIYLGRNPFLDQHYNVPPNWYP 67 Query: 67 FLYHLIVAIFAKIVGIEKSMLML--QAVFSLLMLLIMYWIAKNLWGETAGIFVLSLSLLL 124 FLYH ++AI AK I LM+ +FS++M+ Y + + L + G+ + SLS L+ Sbjct: 68 FLYHSLIAILAKFTSIPIWTLMIWTPLIFSIIMVFAWYKLGRELNEKYGGLLLGSLSFLI 127 Query: 125 LPAHRYPNPKELAPILGMISLYFSLR-----RKYYLG--GLFAGLSLWTHYAVAAPLITF 177 + + +PNPK L PI S LR +K +LG GLF GL +WTHY VA P++ Sbjct: 128 MKSQLFPNPKALIPIFLSASYLAFLRYYRDWKKKHLGYAGLFLGLMMWTHYGVALPVLIA 187 Query: 178 FLVLAIKNRKYMISLAL----ALLTFSPFIVNVILHAQ---FLLPHVEDIYRFWITDTLE 230 L+ ++ K SL L AL+ FSPFI+NVI + + L+ ++ +++T+ Sbjct: 188 ILLYSLFKFKEEKSLVLVPIIALVIFSPFIINVIANIEDGASLMVEGWNVASLSLSNTIR 247 Query: 231 RKLSSLLPPLYLIPLIFLALLRMYKEKDENAMKLFLFISI-IAGMRLFPEIL-KPFGIDL 288 R + PP++ LI L+LL M K +D LF+ I I G+ L P IL GI++ Sbjct: 248 R----IFPPVWGAILILLSLLYMNKAQDRIFWNFVLFVVIGIIGVNLLPSILFITTGIEI 303 Query: 289 WSSRFIGLLPYSYVLLASYGVRSL--------NGEILNVMALTLIPVFASLNFWYSVIND 340 + SRF L Y+Y+LL + L N +++ + + V+ SL+F + ++ Sbjct: 304 FPSRFAISLHYTYLLLYLCAILGLVESKYIRGNKKLVTYLISLIFLVYGSLSFVHYNFHN 363 Query: 341 PFIQASSVELNEYFPQEHFVDVA-----------EWIVKNTARDDVIGAGEEMGMMINAL 389 P S+ + F D+A WI NT RDD + I Sbjct: 364 P----SAPPFLPNYTYVEFEDLAGNYTRGLLIISSWIRNNTGRDDCLIGYPYTLEWIAGF 419 Query: 390 TGRPIIATMYGHGNIFLNNTERRHELKLLFLGNCSEKIIVLQKYRVKYVIVDP 442 TGRP++A YGHGN FLN +RRH++K F + S++ + +KY VKYVI+DP Sbjct: 420 TGRPVVAVSYGHGNPFLNMRQRRHDIK-EFFRDPSKRTAIAEKYGVKYVILDP 471 >pir||G71055 hypothetical protein PH1141 - Pyrococcus horikoshii >gi|3257558|dbj|BAA30241.1| (AP000005) 502aa long hypothetical protein [Pyrococcus horikoshii] Length = 502 Score = 236 bits (595), Expect = 5e-61 Identities = 161/472 (34%), Positives = 253/472 (53%), Gaps = 32/472 (6%) Query: 1 MKNKRA--LFLIISWALIFLPYVLTRKSPPGYIIGGDTLVHAAIARGIYLGRNPFLDQTY 58 MK K+A L+L+ ++ + RK P G GGDT++H A+ RGIYLGRNPFLDQ Y Sbjct: 5 MKRKKAQLLWLLFIAFIVNFSVIYGRKMPVGGN-GGDTIIHMAMIRGIYLGRNPFLDQHY 63 Query: 59 NVPPNWYPFLYHLIVAIFAKI--VGIEKSMLMLQAVFSLLMLLIMYWIAKNLWGETAGIF 116 NVPPNWYPFLYH ++A A++ + I M+ +F+L+M+ Y + + L + G+ Sbjct: 64 NVPPNWYPFLYHALIAFLARLTHISIWSLMIWTPLIFALIMVFAWYKLGEELDKDYGGLL 123 Query: 117 VLSLSLLLLPAHRYPNPKELAPI-LGMISL----YFSLRRKYYL--GGLFAGLSLWTHYA 169 SL+ L L + +PNPKEL PI L + L YF R +L GL GL +W+HY Sbjct: 124 FGSLAFLALKSQLFPNPKELIPIFLPLFYLEYYRYFRDRENRHLLYSGLLLGLMMWSHYG 183 Query: 170 VAAPL----ITFFLVLAIKNRKYMISLALALLTFSPFIVNVILHAQFLLPHVEDIYRFWI 225 A P+ I + L+ +N K MI L+L FSPFIVNV+LH + + W+ Sbjct: 184 AALPVLAGTIAYALIELKRNPKLMIPPFLSLAVFSPFIVNVLLHIHPGSTMI--VEGKWL 241 Query: 226 TD-TLERKLSSLLPPLYLIPLIFLALLRMYKEKDENAMKLFLFISIIAGMRLFPEILKPF 284 + + L + P + +P++ +A L + K E L + I + G+ + P I+ Sbjct: 242 SALSWSSALHRIASPSWTLPIVTIAFLSLRKRNLEFWNFLIVVIISMIGINVTPTIVYDL 301 Query: 285 -GIDLWSSRFIGLLPYSYVLLASYGVRSL-------NGEILNVMALTLIPVFASLNFW-- 334 G ++ SRF L Y+Y++L +G+ L +IL++ L+ ++ S +F Sbjct: 302 GGPAVFPSRFGIPLSYTYLILCGFGIDFLIKALKIPRKQILSLTFSFLLIIYGSWSFIAC 361 Query: 335 -YSVINDPFIQASSVELNEYFPQEHFVDVAEWIVKNTARDDVIGAGEEMGMMINALTGRP 393 Y + + + +L + + V V+EWI +NT RD + I TGRP Sbjct: 362 NYHNFSSKYTYKNFEDLAGNY-TKGLVKVSEWIRENTERDAYLIGYPYTLEWIAGFTGRP 420 Query: 394 IIATMYGHGNIFLNNTERRHELKLLFLGNCSEKIIVLQKYRVKYVIVDPLTE 445 ++A YGHGN FL+ +RR +++ F N ++ +++KY +KY+I+DP T+ Sbjct: 421 VVAVSYGHGNPFLDMEQRRKDIEEFFT-NPEKREEIIKKYNIKYIILDPYTK 471 >pir||C69078 conserved hypothetical protein MTH1583 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|1272330|gb|AAC44810.1| (U51624) orf2 [Methanobacterium thermoautotrophicum] >gi|2622706|gb|AAB86056.1| (AE000918) conserved protein [Methanobacterium thermoautotrophicum] Length = 465 Score = 71.0 bits (171), Expect = 3e-11 Identities = 116/494 (23%), Positives = 207/494 (41%), Gaps = 66/494 (13%) Query: 4 KRALFLIISWALIFLPYVLTRKSPPGYIIGGDTLVHAAIARGIYLGRN-PFLDQTYNVP- 61 KR +FLII AL L + D H A +Y+GR F D + P Sbjct: 6 KRDVFLIILPALCAFALALIPTFRYTVPLTWDVYYHIHNAT-LYMGRGIVFWDPLTSAPH 64 Query: 62 --PNWYPFLYHLIVAIFAKIVGIEKSML--MLQAVFSLLMLLIMYWIAKNLWGETAGIF- 116 P +YP L+H+++ +KI G+ + +Q VF+ L+LL +++ L+G AG Sbjct: 65 GRPLYYPPLFHIMLLFLSKITGLGFFTVSRFMQPVFAFLILLSFSYVSGKLYGPLAGFLT 124 Query: 117 -VLSLSLLLLPAHRYPNPKELAPILGMISLYFSLR------RKY-YLGGLFAGLSLWTHY 168 + S L +P P+ LA I + +Y R R Y L GL GL L TH Sbjct: 125 GMFLFSSLFFMRMMFPIPESLAMISIPLFVYGYYRSMECCSRLYPVLTGLLLGLMLMTHI 184 Query: 169 AVAAPLITFFLVLAIKNRKYMISLALALLTFSPFIVNVILHAQFLLPHV---------ED 219 A+ ++LA+ ++ L + P+ + +IL + LL V Sbjct: 185 LSAS------IILAVVTLSTIV-LYIGKYDIRPWNLLIILGSGLLLASVWYAPLIVKYGF 237 Query: 220 IYRFWITDTL--ERKLSSLLPPLYLIPLIFLALLRMYKEKDENAMKLF-LFISIIAGMRL 276 ++R T +L E ++ L L ++ L + +EK + M ++ +F+ + L Sbjct: 238 VFRSPATSSLPLEGYITHLGVLLTILSLAGIVHAAGRREKKDVIMAVWTVFVLALTAAYL 297 Query: 277 FPEILKPFGIDLWSSRFIGLLPYSYVLLASY---GVRSLNGEILNVMALTLIPVFASLNF 333 G+ + S R + + L++ +R NG+ + LI ++ Sbjct: 298 L-------GLKVLSDRILYFALFPMATLSANLFNSIRINNGKWSFYAIILLISLYC---- 346 Query: 334 WYSVINDPFIQASSVELNEYFPQEHFVDVAEWIVKNTARDDVIGAGEEMGMMINALTGRP 393 I D + ASS++L + +D+AEW N V+ + + I A++G+P Sbjct: 347 ----IYDGYSLASSIKLEV---SDSELDIAEWFRYNGDHATVVVSDYHIQPAIVAVSGQP 399 Query: 394 IIATMYGHGNIFLNNTERRHELKLLFLGNCSEKIIVLQKYRVKYVIVDPLTEAKNMSCVG 453 + A Y G I N E+ ++ + + + + RV Y+I+D + Sbjct: 400 VSAGGYAPGTIRSINVEK-------YISSFNYTKHDIMRDRVGYIILD---NEERTPPYS 449 Query: 454 KVIFRKGDVEIVKV 467 ++++R D + +V Sbjct: 450 RLVYRNRDFSVYRV 463 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.329 0.145 0.443 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 173409764 Number of Sequences: 2977 Number of extensions: 7178854 Number of successful extensions: 21563 Number of sequences better than 1.0e-10: 5 Number of HSP's better than 0.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 21540 Number of HSP's gapped (non-prelim): 5 length of query: 470 length of database: 189,106,746 effective HSP length: 51 effective length of query: 419 effective length of database: 158,583,909 effective search space: 66446657871 effective search space used: 66446657871 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 40 (21.7 bits) S2: 167 (69.5 bits)