BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1701 (truA) DE:pseudouridylate synthase I (truA)
(263 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75076 pseudouridylate synthase I (trua) PAB1701 - Pyrococc... 529 e-149
sp|O58941|TRUA_PYRHO PROBABLE TRNA PSEUDOURIDINE SYNTHASE A (PSE... 443 e-124
gi|11499319 pseudouridylate synthase I (truA) # [Archaeoglobus f... 171 6e-42
sp|O26928|TRUA_METTH PROBABLE TRNA PSEUDOURIDINE SYNTHASE A (PSE... 158 6e-38
sp|Q59069|TRUA_METJA PROBABLE TRNA PSEUDOURIDINE SYNTHASE A (PSE... 141 8e-33
emb|CAC12061.1| (AL445066) tRNA-pseudouridine synthase I related... 132 5e-30
sp|Q9Z9J0|TRUA_BACHD TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 128 6e-29
sp|Q9X1R0|TRUA_THEMA TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 125 7e-28
sp|P45291|TRUA_HAEIN TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 115 4e-25
pir||A82253 tRNA pseudouridine synthase A VC0999 [imported] - Vi... 115 4e-25
gb|AAF26999.1|AC016827_10 (AC016827) putative tRNA pseudouridine... 109 3e-23
sp|P70973|TRUA_BACSU TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 108 1e-22
sp|O66953|TRUA_AQUAE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 106 4e-22
sp|P57295|TRUA_BUCAI TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 106 4e-22
sp|O87016|TRUA_PSEAE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 104 9e-22
sp|O84469|TRUA_CHLTR TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 103 3e-21
pir||D81669 tRNA pseudouridine synthase A TC0748 [imported] - Ch... 102 3e-21
sp|P07649|TRUA_ECOLI TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 102 6e-21
pdb|1DJ0|A Chain A, The Crystal Structure Of E. Coli Pseudouridi... 102 6e-21
sp|P70830|TRUA_BORBU TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 98 1e-19
emb|CAA70351.1| (Y09141) pseudouridylate synthase I [Borrelia bu... 97 2e-19
gb|AAG20174.1| (AE005094) pseudouridylate synthase I; TruA [Halo... 97 2e-19
pir||A81014 tRNA pseudouridine synthase A NMB2036 [imported] - N... 96 3e-19
sp|Q9Z7X4|TRUA_CHLPN TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 96 4e-19
pir||G81956 pseudouridylate synthase (EC 4.2.1.70) tRNA-specific... 96 4e-19
pir||T50192 probable pseudouridylate synthase [imported] - fissi... 95 1e-18
pir||E81605 tRNA pseudouridine synthase A CP0168 [imported] - Ch... 94 1e-18
pir||D75291 pseudouridylate synthase I - Deinococcus radiodurans... 93 3e-18
pir||B82879 pseudouridylate synthase I UU536 [imported] - Ureapl... 90 3e-17
sp|P73295|TRUA_SYNY3 TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 89 4e-17
sp|Q45557|TRUA_BACSP TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 88 1e-16
gb|AAF91402.1|AF266505_1 (AF266505) pseudouridine synthase 3 [Mu... 83 3e-15
sp|P47428|TRUA_MYCGE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 82 5e-15
gb|AAF23795.1|AF213822_10 (AF213822) tRNA-pseudouridine synthase... 82 7e-15
sp|Q9ZCA3|TRUA_RICPR TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 80 2e-14
sp|Q50291|TRUA_MYCPN TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 79 6e-14
gi|6321112 Depressed growth-rate protein; Deg1p [Saccharomyces c... 77 2e-13
pir||C81355 probable pseudouridylate synthase (EC 4.2.1.70) tRNA... 76 3e-13
pir||A82691 tRNA pseudouridine synthase A XF1373 [imported] - Xy... 76 3e-13
sp|Q09524|YQN3_CAEEL PROBABLE PSEUDOURIDYLATE SYNTHASE E02H1.3 (... 76 5e-13
pir||S69220 pseudouridine synthase 1 - yeast (Saccharomyces cere... 73 4e-12
sp|O06322|TRUA_MYCTU TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 72 6e-12
gb|AAF55785.1| (AE003731) CG4159 gene product [Drosophila melano... 71 2e-11
sp|Q9ZKC0|TRUA_HELPJ TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 69 7e-11
sp|P56144|TRUA_HELPY TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYL... 68 9e-11
>pir||D75076 pseudouridylate synthase I (trua) PAB1701 - Pyrococcus abyssi
(strain Orsay) >gi|5458425|emb|CAB49913.1| (AJ248286)
pseudouridylate synthase I (truA) [Pyrococcus abyssi]
Length = 263
Score = 529 bits (1348), Expect = e-149
Identities = 263/263 (100%), Positives = 263/263 (100%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV
Sbjct: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
Query: 61 VAITTEDEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIGLEVEKV 120
VAITTEDEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIGLEVEKV
Sbjct: 61 VAITTEDEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIGLEVEKV 120
Query: 121 KECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRIVKA 180
KECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRIVKA
Sbjct: 121 KECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRIVKA 180
Query: 181 LELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPLNDKALKKFKR 240
LELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPLNDKALKKFKR
Sbjct: 181 LELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPLNDKALKKFKR 240
Query: 241 EVEERFRQKIMGAYLLWDMIQLL 263
EVEERFRQKIMGAYLLWDMIQLL
Sbjct: 241 EVEERFRQKIMGAYLLWDMIQLL 263
>sp|O58941|TRUA_PYRHO PROBABLE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7450404|pir||D71068 probable pseudouridylate
synthase - Pyrococcus horikoshii
>gi|3257659|dbj|BAA30342.1| (AP000005) 262aa long
hypothetical pseudouridylate synthase [Pyrococcus
horikoshii]
Length = 262
Score = 443 bits (1128), Expect = e-124
Identities = 206/262 (78%), Positives = 245/262 (92%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
M+VA KIAYDGT+FHGFQRQPN+RTVEGEIIKALNNSGIM+++ KSASRTD+GVSALGNV
Sbjct: 1 MKVALKIAYDGTKFHGFQRQPNVRTVEGEIIKALNNSGIMFNELKSASRTDKGVSALGNV 60
Query: 61 VAITTEDEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIGLEVEKV 120
VAITTEDE+ NPM+LNA+L+DVW+LSA++VP DFHPRFWA+SKVYRYYLPS L+VEKV
Sbjct: 61 VAITTEDERITNPMILNAKLKDVWVLSAVKVPSDFHPRFWARSKVYRYYLPSFDLDVEKV 120
Query: 121 KECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRIVKA 180
KECS+LFLGVHDFSAFSRVDGR+TVRSIDRIEV LGP+L IE+EAKSFLWEMVRRIVK
Sbjct: 121 KECSKLFLGVHDFSAFSRVDGRETVRSIDRIEVLDLGPVLAIEVEAKSFLWEMVRRIVKT 180
Query: 181 LELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPLNDKALKKFKR 240
LELCGLG+LS EE+++ML GKFEKS+KVPPAP EGLLLV++KY+GIEFP++DKAL+KF++
Sbjct: 181 LELCGLGKLSSEEVRKMLRGKFEKSRKVPPAPAEGLLLVEVKYKGIEFPIHDKALEKFRK 240
Query: 241 EVEERFRQKIMGAYLLWDMIQL 262
++E+RFR+KI AYLLWDM ++
Sbjct: 241 DMEKRFREKIARAYLLWDMTKI 262
>gi|11499319 pseudouridylate synthase I (truA) # [Archaeoglobus fulgidus]
>gi|3915163|sp|O28544|TRUA_ARCFU PROBABLE TRNA
PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7450401|pir||A69466 pseudouridylate synthase I
(truA) homolog - Archaeoglobus fulgidus
>gi|2648818|gb|AAB89516.1| (AE000983) pseudouridylate
synthase I (truA) # [Archaeoglobus fulgidus]
Length = 265
Score = 171 bits (430), Expect = 6e-42
Identities = 100/248 (40%), Positives = 147/248 (58%), Gaps = 7/248 (2%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
M+ AFKIAY G FHG Q QP+ RTVEGE+I AL G+ +SA RTD GV A G V
Sbjct: 2 MKFAFKIAYFGDNFHGSQFQPDQRTVEGEVINALRRLGVENPRLRSAGRTDAGVHAYGQV 61
Query: 61 VAITTEDEKALNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIGLEVEK 119
++ +ED+ + P +LNA L ED+ + +V +DF PR AKS+VY Y + ++
Sbjct: 62 ISFYSEDK--IFPRMLNAELPEDITAWAWAKVSEDFDPR-RAKSRVYTYVMYGSDYDISA 118
Query: 120 VKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRI 177
+++ + +GVHDFS F++ +G VR I ++ +I EIE +F W MVR I
Sbjct: 119 MRKAVKELIGVHDFSNFTKKFGEGESCVREIISADIRADREFIIFEIEGNAFTWNMVRCI 178
Query: 178 VKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPLNDKALKK 237
V A+ G S E +++L + K ++V PAPP GL+L D+KY+ +EF ++D A K
Sbjct: 179 VTAIMEIGKQHRSIEWFRDLLNPEKHK-ERVEPAPPYGLILKDVKYDDVEFEIDDYAFKT 237
Query: 238 FKREVEER 245
+ +E+R
Sbjct: 238 LQSRIEDR 245
>sp|O26928|TRUA_METTH PROBABLE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7450402|pir||B69212 pseudouridylate synthase I -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2621931|gb|AAB85338.1| (AE000861) pseudouridylate
synthase I [Methanobacterium thermoautotrophicum]
Length = 275
Score = 158 bits (396), Expect = 6e-38
Identities = 94/253 (37%), Positives = 154/253 (60%), Gaps = 12/253 (4%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSD----FKSASRTDRGVSAL 57
++A K+AY GT +HGFQRQP++ TVEG++++AL +GI+ F+ A RTDRGV AL
Sbjct: 3 KIALKVAYIGTNYHGFQRQPDVPTVEGKLLEALEGAGIIEDPKRARFQIAGRTDRGVHAL 62
Query: 58 GNVVAITTEDEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYLP-SIGLE 116
GN V+ TE++ +N + + D+ +L+ V F R+ + YRY L ++
Sbjct: 63 GNFVSFFTEEDIHVN-QINDLLPRDIRVLAWASVMYPFKVRY-PLERHYRYILHREESMD 120
Query: 117 VEKVKECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRR 176
+ E + F G HDFS FSR RD VR I+ + + +G +II++ +SFLW+MVR+
Sbjct: 121 TYSMAEAAAHFRGTHDFSNFSRRSDRDPVRRINDVRISEVGDSIIIDVYGESFLWQMVRK 180
Query: 177 IVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPL--NDKA 234
+V+AL + G L+ +++ +L+ ++ + P PPE L+L+D+KY G++ L ++ A
Sbjct: 181 MVRALLMVSEGELAPDDMAGLLD--TDRRVFLEPMPPENLILMDLKY-GVKIKLRHDEYA 237
Query: 235 LKKFKREVEERFR 247
K+F +EE F+
Sbjct: 238 FKRFISLLEEEFK 250
>sp|Q59069|TRUA_METJA PROBABLE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|2129330|pir||A64509 tRNA-pseudouridine synthase I
(EC 5.4.99.12) - Methanococcus jannaschii
>gi|1500579|gb|AAB99697.1| (U67608) pseudouridylate
synthase I (truA) [Methanococcus jannaschii]
Length = 259
Score = 141 bits (352), Expect = 8e-33
Identities = 94/253 (37%), Positives = 149/253 (58%), Gaps = 18/253 (7%)
Query: 6 KIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSD--FKSASRTDRGVSALGNVVAI 63
KIAYDG + FQ+QP+ +TV ++ AL +G + S RTD+GVSALGN V +
Sbjct: 5 KIAYDGR--YSFQQQPHKQTVCDILLDALEETGFLAKKKVIYSGGRTDKGVSALGNFVVV 62
Query: 64 TTEDEKALNPMVLNARLED--VWILSAIEVPQDFHPRFWAKSKVYRYYLPSIGLEVEKVK 121
+ E L+ +NA+L+D +W+L E+ D P+ K + YRY LP+IG +V+ +K
Sbjct: 63 ELKKEPILS--YINAKLKDKGIWVLGYREI--DEIPK--VKYRHYRYILPNIGYDVDAIK 116
Query: 122 ECSQLFLGVHDFSAFSRVDG---RDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRIV 178
E ++ +G H F S+ D + +R+I I++ L ++I +SFLW MVR+IV
Sbjct: 117 EGAKKMIGTHSFHNLSKRDRTKEKSPIRTIYDIKISENEFYLTVDIIGESFLWNMVRKIV 176
Query: 179 KALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPLNDKALKKF 238
AL+L G G+ E I ++L+ + VP PPEGL+LV+ K + IE+ ++ +++KF
Sbjct: 177 GALDLVGRGKKPVEWIDKLLDENHREG--VPTFPPEGLILVEAKVD-IEYIYDNYSIRKF 233
Query: 239 KREVEERFRQKIM 251
K E F+ ++M
Sbjct: 234 KEYWGEFFKLQVM 246
>emb|CAC12061.1| (AL445066) tRNA-pseudouridine synthase I related protein
[Thermoplasma acidophilum]
Length = 257
Score = 132 bits (328), Expect = 5e-30
Identities = 90/253 (35%), Positives = 131/253 (51%), Gaps = 17/253 (6%)
Query: 4 AFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVVAI 63
AFK Y+G+ F GFQR +VE II L SG Y+ K+A+RTDR VSAL NV ++
Sbjct: 10 AFKFGYEGSYFTGFQRGNGDHSVEDTIIDTLKASGFDYA-IKAAARTDRYVSALSNVFSL 68
Query: 64 TTEDEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIGLEVEKVKEC 123
T ++N+R++ +++ S EVP DF+PR K YRYY+ L+ +
Sbjct: 69 ETGRSPEAVAGLINSRIDHIYVHSYAEVPDDFNPRH-CTFKTYRYYILRQDLDCISLDIN 127
Query: 124 SQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRIVKALEL 183
F+G HDF F R D R+TVR+I +L +E A+SFLW +R +V +
Sbjct: 128 LGKFIGTHDFRRFVRADSRNTVRTIINASCKNDNGLLYLEFSARSFLWNQIRTMVAFI-- 185
Query: 184 CGLGRLSCEEIKEMLEGKFEKSKKVPP-APPEGLLLVDIKYEGIEFPLNDKALKKFKREV 242
L + E F S++ P A + LLL DI Y+GIEF + +KK +++
Sbjct: 186 --LDNIGVET------DPFSTSERYPRVARAQNLLLWDIYYDGIEF----RKVKKIPKKM 233
Query: 243 EERFRQKIMGAYL 255
+ + IM L
Sbjct: 234 QGVYENSIMNYIL 246
>sp|Q9Z9J0|TRUA_BACHD TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|11269487|pir||T44415 pseudouridylate synthase I truA
[imported] - Bacillus halodurans
>gi|4512436|dbj|BAA75303.1| (AB017508) truA homologue
(identity of 62% to B. subtilis ) [Bacillus halodurans]
>gi|10172779|dbj|BAB03886.1| (AP001507) tRNA
pseudouridine synthase A (pseudouridylate synthase I)
[Bacillus halodurans]
Length = 263
Score = 128 bits (319), Expect = 6e-29
Identities = 88/238 (36%), Positives = 126/238 (51%), Gaps = 23/238 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSAS-RTDRGVSALGNV 60
R+ K+AYDGT F G+Q QPN RTV+GE+ L N S +AS RTD GV A G +
Sbjct: 3 RIGLKVAYDGTDFAGYQIQPNERTVQGELESVLKNIHKGMSIRVTASGRTDTGVHARGQI 62
Query: 61 VAITTEDEKALN--PMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRY--------- 108
V T ++ P+ LN++L D+ +L A +VP DFH R+ AK+K YRY
Sbjct: 63 VHFDTSLSFPVDRWPIALNSQLPADICVLEAADVPADFHARYSAKTKEYRYRVLTSAQAD 122
Query: 109 -------YLPSIGLEVEKVKECSQLFLGVHDFSAF--SRVDGRDTVRSIDRIEVFTLGPI 159
Y L+VE ++ + LG HDFS+F ++ + D VR+I+ + ++ G
Sbjct: 123 VFRRNYTYHVRYPLDVEAMQRAAVQLLGTHDFSSFCAAKAEVEDKVRTIEDVALWREGDE 182
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLL 217
LI I FL+ MVR IV L G G+ S EE+ ++L + + AP GL+
Sbjct: 183 LIFSIRGNGFLYNMVRIIVGTLLEIGAGKRSAEEVAKILAAR-SREAAGKTAPGHGLI 239
>sp|Q9X1R0|TRUA_THEMA TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437069|pir||G72237 pseudouridylate synthase I -
Thermotoga maritima (strain MSB8)
>gi|4982143|gb|AAD36641.1|AE001802_10 (AE001802)
pseudouridylate synthase I [Thermotoga maritima]
Length = 245
Score = 125 bits (310), Expect = 7e-28
Identities = 86/248 (34%), Positives = 133/248 (52%), Gaps = 31/248 (12%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
RVA I YDG+ F G+Q QP++RTV+G I AL ++A RTD GV A G ++
Sbjct: 3 RVAAVIEYDGSNFFGYQGQPDVRTVQGVIEDALERIFKQRIYTQAAGRTDTGVHANGQLI 62
Query: 62 A-------ITTEDEKALNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPS- 112
A +TTED + +NA L +DV++ EVP +FHPRF ++Y Y++ +
Sbjct: 63 AFNCPNDRMTTEDIR----NAMNANLPDDVYVKEVFEVPVNFHPRFDVTKRIYHYFILTS 118
Query: 113 ---------------IGLEVEKVKECSQLFLGVHDFSAF-SRVDGRDTVRSIDRIEVFTL 156
L+++ +++ + G HDF++F + D RD VR+I RI + L
Sbjct: 119 RQKNVFLRKYVWWFPYELDLDAMRKAVKYLEGTHDFTSFKTGSDERDPVRTIYRIRILRL 178
Query: 157 -GPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEG 215
+++I +E +SFL MVR IV AL GL + E++KE+LE + ++S AP G
Sbjct: 179 KNDLVLIRVEGRSFLRRMVRNIVAALVKVGLKQWEPEKMKEVLEAR-DRSAAAGTAPAHG 237
Query: 216 LLLVDIKY 223
L + +
Sbjct: 238 LYFYKVLF 245
>sp|P45291|TRUA_HAEIN TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|1075190|pir||F64134 tRNA-pseudouridine synthase I
(EC 5.4.99.12) - Haemophilus influenzae (strain Rd KW20)
>gi|1574492|gb|AAC23291.1| (U32837) pseudouridylate
synthase I (truA) [Haemophilus influenzae Rd]
Length = 269
Score = 115 bits (286), Expect = 4e-25
Identities = 82/245 (33%), Positives = 126/245 (50%), Gaps = 24/245 (9%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
M++A I Y+G ++G+QRQ +R+V+ E+ KAL++ + A RTD GVS G V
Sbjct: 1 MKIALGIEYNGQNYYGWQRQEKVRSVQEELEKALSHIANEKIEIFCAGRTDSGVSGTGQV 60
Query: 61 VAITT---EDEKALNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYL------ 110
V T EKA NA L +D+ + A +V +FH RF A ++ YRY L
Sbjct: 61 VHFETNAVRPEKAW-AFGTNAHLPDDIAVSWAKQVDDEFHARFSATARRYRYILYCNKLR 119
Query: 111 ----------PSIGLEVEKVKECSQLFLGVHDFSAF--SRVDGRDTVRSIDRIEVFTLGP 158
+ L+ EK+ + Q LG DFS+F ++ R++ + V +G
Sbjct: 120 SAILAGGITHCHLDLDAEKMHQAGQCLLGEQDFSSFRAAQCQSHTPWRNVHHLNVSRIGK 179
Query: 159 ILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLL 218
+I++I+A +F+ MVR IV +L G G E ++ +LE K + P A P+GL L
Sbjct: 180 YIIVDIQANAFVHHMVRNIVGSLIEVGTGNQPIEWMQWLLEQK-NRQLAAPTAKPDGLYL 238
Query: 219 VDIKY 223
VD+ Y
Sbjct: 239 VDVIY 243
>pir||A82253 tRNA pseudouridine synthase A VC0999 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9655462|gb|AAF94160.1|
(AE004182) tRNA pseudouridine synthase A [Vibrio
cholerae]
Length = 264
Score = 115 bits (286), Expect = 4e-25
Identities = 80/244 (32%), Positives = 125/244 (50%), Gaps = 22/244 (9%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
MR+A I YDGT ++G+QRQ +++V+ + KAL+ + + A RTD GV G V
Sbjct: 1 MRIALGIEYDGTHYYGWQRQREVKSVQEALEKALSKIANHPVEVQCAGRTDAGVHGTGQV 60
Query: 61 VAITTEDEKALNPMVLNARL---EDVWILSAIEVPQDFHPRFWAKSKVYRY------YLP 111
V T E+ + + A +D+ + A+ VP +FH RF A ++ YRY Y P
Sbjct: 61 VHFDTTAERQMVAWTMGANANLPKDIAVRWAMAVPDEFHARFSATARRYRYVIYNHVYRP 120
Query: 112 SI----------GLEVEKVKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPI 159
I L+VEK+++ Q LG +DF++F V R R++ + V G
Sbjct: 121 GILNSGVSHYHGELDVEKMQQAGQYLLGENDFTSFRAVHCQSRSPWRNVMHLNVTRHGRY 180
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
++I+I+A +F+ MVR I +L G G E I+ +L K +++ A EGL LV
Sbjct: 181 VVIDIKANAFVHHMVRNITGSLIAVGRGEQKPEWIQWLLAQK-DRTLAAATAKAEGLYLV 239
Query: 220 DIKY 223
+ Y
Sbjct: 240 SVDY 243
>gb|AAF26999.1|AC016827_10 (AC016827) putative tRNA pseudouridine synthase [Arabidopsis
thaliana]
Length = 298
Score = 109 bits (271), Expect = 3e-23
Identities = 88/257 (34%), Positives = 125/257 (48%), Gaps = 32/257 (12%)
Query: 7 IAYDGTRFHGFQRQPNLRTVEGEIIKAL---NNSGIMYSDFKSASRTDRGVSALGNVVAI 63
IAYDGTRF G+Q Q + T++ + KAL G A RTD GV A G V
Sbjct: 22 IAYDGTRFAGWQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHF 81
Query: 64 TTE-DEKALNPM--VLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRY----------- 108
T + +L+ LN L +D+ + +FH RF A SKVYRY
Sbjct: 82 VTPFNYTSLDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPF 141
Query: 109 -----YLPSIGLEVEKVKECSQLFLGVHDFSAFS---RVDG-RDTVRSIDRIEVFTLGPI 159
Y + L K++E + LF+G HDFSAF+ R DG D +++I R +V +G +
Sbjct: 142 QRHWAYHCAYKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSL 201
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEK---SKKVPPAPPEGL 216
L +E+E FL+ VR +V L G L + + +LE K + PA P GL
Sbjct: 202 LQLEVEGSGFLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGL 261
Query: 217 LLVDIKY--EGIEFPLN 231
LV +KY + ++ PL+
Sbjct: 262 CLVSVKYKEDHLKLPLD 278
>sp|P70973|TRUA_BACSU TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437066|pir||F69726 pseudouridylate synthase I truA
- Bacillus subtilis >gi|1644206|dbj|BAA10987.1| (D64126)
unknown [Bacillus subtilis] >gi|2632415|emb|CAB11924.1|
(Z99104) pseudouridylate synthase I [Bacillus subtilis]
Length = 247
Score = 108 bits (266), Expect = 1e-22
Identities = 82/250 (32%), Positives = 124/250 (48%), Gaps = 30/250 (12%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSD-----FKSASRTDRGVS 55
MR+ I+YDG F+G+Q QP RTV+ E+ KAL +++ S+ RTD GV
Sbjct: 1 MRLKCTISYDGHLFNGYQVQPGKRTVQDELEKAL---AVLHKSKDRIPVVSSGRTDSGVH 57
Query: 56 ALGNVVAITTEDEKALN--PMVLNARLEDVWILSAIEVPQD-FHPRFWAKSKVYRYYLP- 111
A G V+ T P LNA L D + E+ D FH RF A K YRY++
Sbjct: 58 AAGQVIHFDTPLSIPAERWPYALNALLPDDIAVKQAEIADDGFHARFSAVKKEYRYFVYT 117
Query: 112 ---------------SIGLEVEKVKECSQLFLGVHDFSAF--SRVDGRDTVRSIDRIEVF 154
S L V+ ++E ++ +G HDF++F ++ + +D VR+I ++
Sbjct: 118 EKHPDVFKRHYAYHFSYRLNVQDMREAAKHPIGTHDFTSFCAAKTEVQDKVRTIYELDWT 177
Query: 155 TLGPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPE 214
L + I FL+ MVR I L G+G++S +E+K MLE K ++ AP
Sbjct: 178 ETADGLQMRITGSGFLYNMVRIIAGTLLDAGIGKISPDEVKSMLEAK-DREAAERTAPGH 236
Query: 215 GLLLVDIKYE 224
GL L ++ Y+
Sbjct: 237 GLYLWNVYYD 246
>sp|O66953|TRUA_AQUAE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437068|pir||F70365 pseudouridine synthase I -
Aquifex aeolicus >gi|2983318|gb|AAC06908.1| (AE000705)
pseudouridine synthase I [Aquifex aeolicus]
Length = 241
Score = 106 bits (261), Expect = 4e-22
Identities = 83/237 (35%), Positives = 126/237 (53%), Gaps = 31/237 (13%)
Query: 6 KIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFK--SASRTDRGVSALGNVVAI 63
++A+ GT F+G+Q QPNLRTV+GEI KAL S I+ D K RTD GV AL +
Sbjct: 7 RLAFVGTNFYGWQVQPNLRTVQGEIQKAL--SQILCEDVKVTGCCRTDSGVHALDYIANF 64
Query: 64 TTE----DEKALNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYL-------P 111
T+ +EK L LN L +DV + + +V ++F+ R+ K KVY Y + P
Sbjct: 65 KTQKDFPEEKLLK--ALNGILPKDVGVYAVKKVSEEFNARYSVKGKVYLYKIWNSEVRNP 122
Query: 112 SI---------GLEVEKVKECSQLFLGVHDFSAFSRV-DGRDTVRSIDRIEVFTLGPILI 161
+ + E ++ + F G HDF A +++ + R+TV +++ + + P++
Sbjct: 123 FLYPFSWQVKREINTEVLRNILKKFEGTHDFRALTKLEEERNTVINLEEVSLNVEYPLIE 182
Query: 162 IEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLL 218
I ++A FL MVRRIV LG S E ++E+L+G K APP+GL L
Sbjct: 183 IRLKASHFLRYMVRRIVGTAVKISLGLYSEEVLEELLQG---KGNSPYTAPPQGLHL 236
>sp|P57295|TRUA_BUCAI TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|10038881|dbj|BAB12916.1| (AP001118) pseudouridylate
synthase I [Buchnera sp. APS]
Length = 266
Score = 106 bits (261), Expect = 4e-22
Identities = 77/241 (31%), Positives = 127/241 (51%), Gaps = 22/241 (9%)
Query: 4 AFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV-- 61
A + YDG+ +HG+QRQ L +++ EI KAL+ D A RTD GV ++G V+
Sbjct: 10 ALGVEYDGSYYHGWQRQKILPSIQEEIEKALSIIANHKIDVVCAGRTDAGVHSIGQVIHF 69
Query: 62 AITTEDEKALNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIG------ 114
T +K+ + +N+ L E++ ++ EV ++FH R+ A ++ YRY + +
Sbjct: 70 KTTANRKKSSWSIGVNSYLSENISVVWVKEVTENFHARYSAITRSYRYIIYNYSLRSAIF 129
Query: 115 ----------LEVEKVKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPILII 162
L V+K+ +Q LG HDF++F + R+I ++ VF +++
Sbjct: 130 QTKLNHIYRKLNVDKMNFEAQFLLGEHDFTSFRALGCQSHSPWRNITKLNVFRFHNWVVV 189
Query: 163 EIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIK 222
+I A SFL MVR IV +L G+ + IK++LE K ++S AP +GL LV ++
Sbjct: 190 DITANSFLHHMVRNIVGSLIEVGISKKKEYWIKDLLEKK-DRSHAGATAPAKGLYLVYVE 248
Query: 223 Y 223
Y
Sbjct: 249 Y 249
>sp|O87016|TRUA_PSEAE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|11352726|pir||E83257 tRNA-pseudouridine synthase I
PA3114 [imported] - Pseudomonas aeruginosa (strain PAO1)
>gi|3237313|gb|AAC23941.1| (U93274) HisT [Pseudomonas
aeruginosa] >gi|9949225|gb|AAG06502.1|AE004735_9
(AE004735) tRNA-pseudouridine synthase I [Pseudomonas
aeruginosa]
Length = 285
Score = 104 bits (258), Expect = 9e-22
Identities = 79/256 (30%), Positives = 128/256 (49%), Gaps = 24/256 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQP-NLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
R+A I Y G+R+ G+QRQ + +V+ + +AL+ A RTD V A G V
Sbjct: 19 RIALGIEYKGSRYRGWQRQEAGVPSVQEALERALSKVAAEPVGVICAGRTDAAVHASGQV 78
Query: 61 VAITTEDEKALNPMVL--NARLE-DVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIG--- 114
V T E+ L V+ NA L D+ + A +P FH RF A ++ YRY + +
Sbjct: 79 VHFDTAVERPLKAWVMGTNANLPADISVTWARVMPAHFHARFSAMARRYRYAIYNDPIRP 138
Query: 115 -------------LEVEKVKECSQLFLGVHDFSAFSRVD--GRDTVRSIDRIEVFTLGPI 159
L++ +++E +Q+ +G HDF++F V + V+++ + + G +
Sbjct: 139 AHLAEEVTWNHRPLDIGRMREAAQVLVGTHDFTSFRAVQCQAKSPVKTMHHVRLLEHGRL 198
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
++++I +FL MVR I L G G E KE+LE + ++ V A P GL LV
Sbjct: 199 IVLDIRGNAFLHHMVRNIAGVLMTIGAGERPIEWAKEVLEARDRRAGGV-TAHPYGLYLV 257
Query: 220 DIKYEGIEFPLNDKAL 235
++Y G EF L ++ L
Sbjct: 258 RVEYPG-EFELPERYL 272
>sp|O84469|TRUA_CHLTR TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437074|pir||C71511 probable pseudouridylate
synthase I - Chlamydia trachomatis (serotype D, strain
UW3/Cx) >gi|3328897|gb|AAC68063.1| (AE001320)
Pseudouridylate Synthase I [Chlamydia trachomatis]
Length = 267
Score = 103 bits (254), Expect = 3e-21
Identities = 75/247 (30%), Positives = 119/247 (47%), Gaps = 27/247 (10%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
++ +IAY GT + G+Q QPN +++ + L S+ RTD GV A G +
Sbjct: 4 KIVLQIAYQGTSYSGWQYQPNALSIQEVLETILKKIAGFRISVISSGRTDAGVHAQGQIA 63
Query: 62 AITTEDEKALNP-----MVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYL----- 110
D +LNA L D+ I A+ +DFH RF A +K YRY L
Sbjct: 64 HFHCPDHPHFTDPRQIQKMLNALLPHDIVIRDAVMTDEDFHSRFSAIAKEYRYTLSLLPK 123
Query: 111 -----------PSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRD---TVRSIDRIEVFTL 156
P L + +++E +Q +G HDF++F+ + GR+ T+R++ +++
Sbjct: 124 PLPHHRLFCFSPRYKLNIARMQEAAQYLVGTHDFASFANL-GREYSSTIRTLYTLDLSEQ 182
Query: 157 GPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGL 216
++ + FL++MVR IV AL G G+ E + +ML K ++ K P APP GL
Sbjct: 183 EHLVTVICRGNGFLYKMVRNIVGALLDIGKGKYPPEHLLDMLATK-DRRKGPPSAPPYGL 241
Query: 217 LLVDIKY 223
L + Y
Sbjct: 242 SLHHVCY 248
>pir||D81669 tRNA pseudouridine synthase A TC0748 [imported] - Chlamydia
muridarum (strain Nigg) >gi|7190776|gb|AAF39555.1|
(AE002343) tRNA pseudouridine synthase A [Chlamydia
muridarum]
Length = 267
Score = 102 bits (253), Expect = 3e-21
Identities = 77/247 (31%), Positives = 120/247 (48%), Gaps = 27/247 (10%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
++ +IAY GT + G+Q QPN +++ + L ++ RTD GV A G V
Sbjct: 4 KIILRIAYQGTAYSGWQYQPNALSIQEVLETLLQKISRTRISVIASGRTDAGVHAQGQVA 63
Query: 62 AITTEDEKALNP-----MVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYL----- 110
D + +LNA L D+ I I +DFH RF A +K Y Y L
Sbjct: 64 HFCCPDHPHFSDPGQIKKMLNALLPHDIVIRDVIATNEDFHSRFSAITKEYHYTLSLLPK 123
Query: 111 -----------PSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRD---TVRSIDRIEVFTL 156
P L +E ++E ++ +G HDF++F+ + GR+ TVR++ +++
Sbjct: 124 PLPHHRLFCFSPRHKLCLESMREAAKYLVGTHDFASFANL-GREYSSTVRTLYALDLLEQ 182
Query: 157 GPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGL 216
++ + + FL++MVR IV AL G G+ E + EMLE K ++ K P APP GL
Sbjct: 183 EHLVTVICKGNGFLYKMVRNIVGALLDIGKGKYPPEHLLEMLEKK-DRKKGPPSAPPYGL 241
Query: 217 LLVDIKY 223
L + Y
Sbjct: 242 SLHHVCY 248
>sp|P07649|TRUA_ECOLI TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE) (PSU-I)
>gi|68342|pir||SYECZ1 tRNA-pseudouridine synthase I (EC
5.4.99.12) [validated] - Escherichia coli
>gi|41718|emb|CAA26522.1| (X02743) pseudouridine
synthase 1 (hisT) (aa 1-270) [Escherichia coli]
>gi|1788657|gb|AAC75378.1| (AE000320) pseudouridylate
synthase I [Escherichia coli K12]
>gi|1799711|dbj|BAA16175.1| (D90863) PSEUDOURIDYLATE
SYNTHASE I (EC 4.2.1.70) (PSEUDOURIDINE SYNTHASE I)
(URACIL HYDROLYASE). [Escherichia coli]
Length = 270
Score = 102 bits (251), Expect = 6e-21
Identities = 77/243 (31%), Positives = 122/243 (49%), Gaps = 22/243 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
++A I YDG++++G+QRQ +R+V+ ++ KAL+ A RTD GV G VV
Sbjct: 11 KIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALSQVANEPITVFCAGRTDAGVHGTGQVV 70
Query: 62 AITTE--DEKALNPMVLNARLE-DVWILSAIEVPQDFHPRFWAKSKVYRYYL------PS 112
T + A + +NA L D+ + VP DFH RF A ++ YRY + P+
Sbjct: 71 HFETTALRKDAAWTLGVNANLPGDIAVRWVKTVPDDFHARFSATARRYRYIIYNHRLRPA 130
Query: 113 I----------GLEVEKVKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPIL 160
+ L+ E++ +Q LG +DF++F V R R++ I V GP +
Sbjct: 131 VLSKGVTHFYEPLDAERMHRAAQCLLGENDFTSFRAVQCQSRTPWRNVMHINVTRHGPYV 190
Query: 161 IIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVD 220
+++I+A +F+ MVR IV +L G I E+L K +++ A EGL LV
Sbjct: 191 VVDIKANAFVHHMVRNIVGSLMEVGAHNQPESWIAELLAAK-DRTLAAATAKAEGLYLVA 249
Query: 221 IKY 223
+ Y
Sbjct: 250 VDY 252
>pdb|1DJ0|A Chain A, The Crystal Structure Of E. Coli Pseudouridine Synthase I
At 1.5 Angstrom Resolution >gi|8569327|pdb|1DJ0|B Chain
B, The Crystal Structure Of E. Coli Pseudouridine
Synthase I At 1.5 Angstrom Resolution
Length = 264
Score = 102 bits (251), Expect = 6e-21
Identities = 77/243 (31%), Positives = 122/243 (49%), Gaps = 22/243 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
++A I YDG++++G+QRQ +R+V+ ++ KAL+ A RTD GV G VV
Sbjct: 5 KIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALSQVANEPITVFCAGRTDAGVHGTGQVV 64
Query: 62 AITTE--DEKALNPMVLNARLE-DVWILSAIEVPQDFHPRFWAKSKVYRYYL------PS 112
T + A + +NA L D+ + VP DFH RF A ++ YRY + P+
Sbjct: 65 HFETTALRKDAAWTLGVNANLPGDIAVRWVKTVPDDFHARFSATARRYRYIIYNHRLRPA 124
Query: 113 I----------GLEVEKVKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPIL 160
+ L+ E++ +Q LG +DF++F V R R++ I V GP +
Sbjct: 125 VLSKGVTHFYEPLDAERMHRAAQCLLGENDFTSFRAVQCQSRTPWRNVMHINVTRHGPYV 184
Query: 161 IIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVD 220
+++I+A +F+ MVR IV +L G I E+L K +++ A EGL LV
Sbjct: 185 VVDIKANAFVHHMVRNIVGSLMEVGAHNQPESWIAELLAAK-DRTLAAATAKAEGLYLVA 243
Query: 221 IKY 223
+ Y
Sbjct: 244 VDY 246
>sp|P70830|TRUA_BORBU TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437067|pir||D70101 pseudouridylate synthase I
(hisT) homolog - Lyme disease spirochete
>gi|2687883|gb|AAC66394.1| (AE001115) pseudouridylate
synthase I (hisT) [Borrelia burgdorferi]
Length = 246
Score = 97.5 bits (239), Expect = 1e-19
Identities = 76/245 (31%), Positives = 112/245 (45%), Gaps = 23/245 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
++ +IAYDG+ +HGFQ QP TV+GEI KAL S+ RTD+GV A ++
Sbjct: 3 KILAEIAYDGSIYHGFQIQPTKPTVQGEIEKALMKINKKKVKIHSSGRTDKGVHAKKQII 62
Query: 62 AITTEDEKALNPM--VLNARL--EDVWILSAIEVPQDFHPRFWAKSKVYRY-------YL 110
+ LN + LNA L + IL V FHPRF A+ + Y Y Y
Sbjct: 63 TFDIKINIQLNNLKKALNAILLKNSIKILKLRYVKNSFHPRFSAQKRKYSYCILNSDNYY 122
Query: 111 PSIG---------LEVEKVKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPI 159
P G L + + + ++ +G HDF+ FS + + R I + G
Sbjct: 123 PWEGYQAHYVNKKLSISNLNQMAKTLIGKHDFTTFSCIKDKSKSKFRHIYFAKFKKRGKY 182
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
+I EI SFLW+MVR I+ + + S + +L+ K ++ AP L L
Sbjct: 183 IIFEIIGSSFLWKMVRSIIGTMLDIEIKNESISTFETILKSK-NRNLARTTAPANALFLE 241
Query: 220 DIKYE 224
+ YE
Sbjct: 242 RVYYE 246
>emb|CAA70351.1| (Y09141) pseudouridylate synthase I [Borrelia burgdorferi]
>gi|2911585|emb|CAA11967.1| (AJ224474) pseudouridylate
synthase I [Borrelia burgdorferi]
Length = 246
Score = 96.7 bits (237), Expect = 2e-19
Identities = 76/245 (31%), Positives = 112/245 (45%), Gaps = 23/245 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
++ +IAYDG+ +HGFQ QP TV+GEI KAL S+ RTD+GV A ++
Sbjct: 3 KILAEIAYDGSIYHGFQIQPTKPTVQGEIEKALMKINKKKVKIHSSGRTDKGVHAKRQII 62
Query: 62 AITTEDEKALNPM--VLNARL--EDVWILSAIEVPQDFHPRFWAKSKVYRY-------YL 110
+ LN + LNA L + IL V FHPRF A+ + Y Y Y
Sbjct: 63 TFDIKINIQLNNLKKALNAILLKNSIKILKLRYVKNSFHPRFSAQKRKYSYCILNSDNYY 122
Query: 111 PSIG---------LEVEKVKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPI 159
P G L + + + ++ +G HDF+ FS + + R I + G
Sbjct: 123 PWEGYQAHYVNKKLSISNLNQMAKTLIGKHDFTTFSCIKDKSKSKFRHIYFAKFKKRGKY 182
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
+I EI SFLW+MVR I+ + + S + +L+ K ++ AP L L
Sbjct: 183 IIFEIIGSSFLWKMVRSIMGTMLDIEIKNESISTFETILKSK-NRNLARTTAPANALFLE 241
Query: 220 DIKYE 224
+ YE
Sbjct: 242 RVYYE 246
>gb|AAG20174.1| (AE005094) pseudouridylate synthase I; TruA [Halobacterium sp.
NRC-1]
Length = 266
Score = 96.7 bits (237), Expect = 2e-19
Identities = 75/247 (30%), Positives = 116/247 (46%), Gaps = 19/247 (7%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNN----SGIMYSDFKSASRTDRGVSAL 57
R AF++AYDG +HGFQRQP++ TV GE+ AL G + +A RTD GVSA
Sbjct: 3 RRAFRVAYDGRPYHGFQRQPDVSTVAGELFGALRRLDVFDGAKPPGYAAAGRTDAGVSAR 62
Query: 58 GNVVAITTEDEKALNPMVLNARLED-VWILSAIEVPQDFHPRF--WAKSKVYRYYLPSIG 114
VA + L P L D + + + + P +FH A++ VY +Y P
Sbjct: 63 AQTVAF--DAPAWLTPDAFTGALPDPIQVWAHADAPPEFHATHDAVARTYVYYWYAPDSR 120
Query: 115 LEVEKVKECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMV 174
++ G HDF + + +TVR +D + G +I + A F E+V
Sbjct: 121 ATDDRAAGALDRLTGTHDFHNLT-PNTTNTVRELD-ATLDRDGAFRVITVRAGGFCRELV 178
Query: 175 RRIVKALELC----GLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYEGIEFPL 230
RR+V ++L R+ E + G VPPA P L+L + Y+G+ F +
Sbjct: 179 RRVVSLVQLVTETGDTDRIDTVLGDEPVAG----PDGVPPADPHPLVLHAVAYDGLSFTV 234
Query: 231 NDKALKK 237
++ A ++
Sbjct: 235 DEDAAER 241
>pir||A81014 tRNA pseudouridine synthase A NMB2036 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7227297|gb|AAF42357.1| (AE002553) tRNA pseudouridine
synthase A [Neisseria meningitidis MC58]
Length = 265
Score = 96.3 bits (236), Expect = 3e-19
Identities = 73/244 (29%), Positives = 120/244 (48%), Gaps = 23/244 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPN-LRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
R A ++YDG+RF+G+Q+Q + + TV+ + AL A RTD GV A V
Sbjct: 8 RWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQIAGEAVSTTVAGRTDTGVHATAQV 67
Query: 61 VAITTEDEKALNPMV--LNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPS----- 112
V T + V +NA L E + +L A +V +FH RF A + YRY L S
Sbjct: 68 VHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVAPEFHARFDAYGRHYRYLLESAPVRS 127
Query: 113 -----------IGLEVEKVKECSQLFLGVHDFSAFSRVD--GRDTVRSIDRIEVFTLGPI 159
+ L++ ++++ + L +G DFS+F + + V++I R ++ +
Sbjct: 128 PLLKNRAGWTHLKLDIGQMRQAAALLVGEQDFSSFRAAECQAKSPVKTIYRADLTQSSGL 187
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
+ +++ +FL MVR I+ AL G GRLS E +++ + + K P P+GL L
Sbjct: 188 VRLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQER-SRLKAPPTFMPDGLYLT 246
Query: 220 DIKY 223
+ Y
Sbjct: 247 GVDY 250
>sp|Q9Z7X4|TRUA_CHLPN TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437073|pir||A72062 pseudouridylate synthase I -
Chlamydophila pneumoniae (strain CWL029)
>gi|4376873|gb|AAD18719.1| (AE001642) Pseudouridylate
Synthase I [Chlamydophila pneumoniae CWL029]
Length = 267
Score = 96.0 bits (235), Expect = 4e-19
Identities = 79/249 (31%), Positives = 117/249 (46%), Gaps = 31/249 (12%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
+VA IAY GT + G+Q+QPN +++ I +L + ++ RTD GV A G V
Sbjct: 3 KVALLIAYQGTAYSGWQQQPNDLSIQEVIESSLKKITKTRTPLIASGRTDAGVHAYGQVA 62
Query: 62 AITTEDEKA-----LNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRY------- 108
D L LNA L +D+ I +FH R+ A +K YRY
Sbjct: 63 HFRAPDHPLFANANLTKKALNAILPKDIVIRDVALFDDNFHARYLAIAKEYRYSLSRLAK 122
Query: 109 ---------YLPSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRD---TVRSIDRIEVFTL 156
Y P E ++E + L +G HDF++F+ GRD TVR+I +++
Sbjct: 123 PLPWQRHFCYTPRHPFSTELMQEGANLLIGTHDFASFAN-HGRDYNSTVRTIYTLDIVDK 181
Query: 157 GPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPP--APPE 214
G L I FL++MVR +V AL G G E + ++LE +K+++ P AP
Sbjct: 182 GDSLSIICRGNGFLYKMVRNLVGALLDVGKGAYPPEHLLDILE---QKNRREGPSAAPAY 238
Query: 215 GLLLVDIKY 223
GL L + Y
Sbjct: 239 GLSLHHVCY 247
>pir||G81956 pseudouridylate synthase (EC 4.2.1.70) tRNA-specific NMA0402
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7379154|emb|CAB83702.1| (AL162753) tRNA
pseudouridine synthase [Neisseria meningitidis Z2491]
Length = 265
Score = 96.0 bits (235), Expect = 4e-19
Identities = 73/244 (29%), Positives = 118/244 (47%), Gaps = 23/244 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPN-LRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
R A ++YDG+RF+G+Q+Q + + TV+ + AL A RTD GV A V
Sbjct: 8 RWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQIAGEAVSTTVAGRTDTGVHATAQV 67
Query: 61 VAITTEDEKALNPMV--LNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPS----- 112
V T + V +NA L E + +L A +V +FH RF A + YRY L S
Sbjct: 68 VHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVAPEFHARFDAYGRHYRYLLESAPVRS 127
Query: 113 -----------IGLEVEKVKECSQLFLGVHDFSAFSRVD--GRDTVRSIDRIEVFTLGPI 159
+ L++E ++ + +G DFS+F + + V++I R ++ +
Sbjct: 128 PLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFSSFRAAECQAKSPVKTIYRADLTQSAGL 187
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
+ +++ +FL MVR I+ AL G GRLS E +++ + + K P P+GL L
Sbjct: 188 VRLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQER-SRLKAPPTFMPDGLYLT 246
Query: 220 DIKY 223
+ Y
Sbjct: 247 GVDY 250
>pir||T50192 probable pseudouridylate synthase [imported] - fission yeast
(Schizosaccharomyces pombe) >gi|6469292|emb|CAB61771.1|
(AL133225) probable pseudouridylate synthase
[Schizosaccharomyces pombe]
Length = 450
Score = 94.8 bits (232), Expect = 1e-18
Identities = 75/251 (29%), Positives = 124/251 (48%), Gaps = 23/251 (9%)
Query: 3 VAFKIAYDGTRFHG--FQRQPN-LRTVEGEIIKALNNSGIMYS----DFKSASRTDRGVS 55
VAFK AY G ++G +Q +P L TVEG++ +AL + ++ F RTD+GVS
Sbjct: 94 VAFKFAYCGWNYNGLAYQLEPTPLPTVEGKVFEALLRAHLITDPSSCSFSRCGRTDKGVS 153
Query: 56 ALGNVVAIT---TEDEKALNPMVLNARLE-DVWILSAIEVPQDFHPRFWAKSKVYRYYL- 110
A+G V+++ +E + VLN L D+ I P+ F RF K + Y+Y
Sbjct: 154 AMGQVISLNVRHSERRPIIYCDVLNRLLPADIRITGYAFPPEGFDARFSCKQRHYKYLFT 213
Query: 111 ---PSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRDTV----RSIDRIEVFTLGP---IL 160
P L+++++ E + L+LG HDF F ++D + R I +V + P +
Sbjct: 214 KDYPGGSLDIDRMNEAAALYLGEHDFRNFCKIDASKQITNYHRRILSSKVICIDPSTGLY 273
Query: 161 IIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPE-GLLLV 219
+++ +FLW VR ++ L L G I ++L+ K +K + E L+L
Sbjct: 274 AFDLQGTAFLWHQVRCMMAILFLIGQKLEPASLINDLLDIKKVPTKPIYDMASEYPLILY 333
Query: 220 DIKYEGIEFPL 230
D ++ IE+ L
Sbjct: 334 DCDFDNIEWTL 344
>pir||E81605 tRNA pseudouridine synthase A CP0168 [imported] - Chlamydophila
pneumoniae (strain AR39) >gi|7189101|gb|AAF38045.1|
(AE002178) tRNA pseudouridine synthase A [Chlamydophila
pneumoniae AR39] >gi|8978952|dbj|BAA98787.1| (AP002547)
pseudouridylate synthase I [Chlamydophila pneumoniae
J138]
Length = 267
Score = 94.4 bits (231), Expect = 1e-18
Identities = 78/249 (31%), Positives = 116/249 (46%), Gaps = 31/249 (12%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
+VA IAY GT + G+Q+QPN +++ I +L + ++ RTD GV A G V
Sbjct: 3 KVALLIAYQGTAYSGWQQQPNDLSIQEVIESSLKKITKTRTPLIASGRTDAGVHAYGQVA 62
Query: 62 AITTEDEKA-----LNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRY------- 108
D L LNA L +D+ I +FH R+ +K YRY
Sbjct: 63 HFRAPDHPLFANANLTKKALNAILPKDIVIRDVALFDDNFHARYLTIAKEYRYSLSRLAK 122
Query: 109 ---------YLPSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRD---TVRSIDRIEVFTL 156
Y P E ++E + L +G HDF++F+ GRD TVR+I +++
Sbjct: 123 PLPWQRHFCYTPRHPFSTELMQEGANLLIGTHDFASFAN-HGRDYNSTVRTIYTLDIVDK 181
Query: 157 GPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPP--APPE 214
G L I FL++MVR +V AL G G E + ++LE +K+++ P AP
Sbjct: 182 GDSLSIICRGNGFLYKMVRNLVGALLDVGKGAYPPEHLLDILE---QKNRREGPSAAPAY 238
Query: 215 GLLLVDIKY 223
GL L + Y
Sbjct: 239 GLSLHHVCY 247
>pir||D75291 pseudouridylate synthase I - Deinococcus radiodurans (strain R1)
>gi|6460099|gb|AAF11837.1|AE002061_4 (AE002061)
pseudouridylate synthase I [Deinococcus radiodurans]
Length = 280
Score = 93.2 bits (228), Expect = 3e-18
Identities = 78/247 (31%), Positives = 115/247 (45%), Gaps = 29/247 (11%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEI---IKALNNSGIMYSDFKSASRTDRGVSA-- 56
R+ +A+DG + G+Q Q N +V+ + + AL G + SA RTD GV A
Sbjct: 17 RLRLTVAWDGRDYAGWQEQRNAVSVQETLQTALHALGGEGALRP--VSAGRTDAGVHAEA 74
Query: 57 --LGNVVAITTEDEKALNPMVLNARLE-DVWILSAIEVPQDFHPRFWAKSKVYRY----- 108
L V T P LNA L V +LSA P FH RF + Y Y
Sbjct: 75 MPLHWDVPTTFRVPLVQLPRALNAWLPASVSVLSAEVAPAGFHARFSCTERRYVYRLWVA 134
Query: 109 ------------YLPSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFTL 156
++P L+ E + +Q +G+HDF+AF+ + R TVR + R+EV
Sbjct: 135 PQRHPLWAGRALHVPG-PLDAEAMNRAAQSLIGLHDFAAFATREDRQTVRDLLRLEVQPQ 193
Query: 157 GPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGL 216
G + I + +SFL MVR +V L L G G+L E++ +L + E+++ PP GL
Sbjct: 194 GELWDIHVAGESFLRHMVRGLVGTLLLVGQGKLGAAEVEGILASR-ERARAGANVPPGGL 252
Query: 217 LLVDIKY 223
+Y
Sbjct: 253 YFAGAEY 259
>pir||B82879 pseudouridylate synthase I UU536 [imported] - Ureaplasma
urealyticum >gi|6899540|gb|AAF30949.1|AE002152_8
(AE002152) pseudouridylate synthase I [Ureaplasma
urealyticum]
Length = 241
Score = 89.7 bits (219), Expect = 3e-17
Identities = 71/248 (28%), Positives = 114/248 (45%), Gaps = 32/248 (12%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEI------IKALNNSGIMYSDFKSASRTDRGV 54
M + YDG+ F+G+ RQP RTV+G++ I +NN I+ + RTD+GV
Sbjct: 1 MNYKISLRYDGSLFYGWARQPQKRTVQGDLEEIFKSIFKINNIRII-----GSGRTDKGV 55
Query: 55 SALGNVVAITTE----DEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYL 110
A ++ D + + + D+ IL +V FH ++ A SK Y+Y +
Sbjct: 56 HAYEQTFSVKHSALKYDSHIIYQALCSRTSSDIQILEVQKVDDSFHAQYNATSKTYQYVI 115
Query: 111 PSIGLEV---------------EKVKECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFT 155
++ +K+ E +LF+G HDF +FS + T+R I+ +++
Sbjct: 116 NDYEFDLFRNNYELFVNQKINDQKILEALELFVGEHDFKSFSTSELTMTIRRINWVKI-K 174
Query: 156 LGPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEG 215
LII I A FL MVR IV + ++S +I E+L +K + AP G
Sbjct: 175 RDTHLIIYINANGFLKNMVRMIVASCLDYAFNKISLAKIHELLIYP-KKGASIKLAPACG 233
Query: 216 LLLVDIKY 223
L L + Y
Sbjct: 234 LYLYKVYY 241
>sp|P73295|TRUA_SYNY3 TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437065|pir||S77476 tRNA-pseudouridine synthase I
(EC 5.4.99.12) - Synechocystis sp. (strain PCC 6803)
>gi|1652401|dbj|BAA17323.1| (D90905) pseudouridine
synthase 1 [Synechocystis sp.]
Length = 275
Score = 89.3 bits (218), Expect = 4e-17
Identities = 75/256 (29%), Positives = 117/256 (45%), Gaps = 30/256 (11%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
RVA + Y GT FHG+QRQPN +++ E+ A+ A RTD GV A V
Sbjct: 11 RVALVMQYLGTHFHGWQRQPNFSSIQEEVELAIAKVVGQPVTLHGAGRTDAGVHAAAQVA 70
Query: 62 AITTEDEKALNP-----MVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYL----- 110
+D P +LN L +D+ + + V +H RF A + YRY +
Sbjct: 71 HF--DDPVGQIPPHRWSKILNGHLPQDIIVRGSAAVDSHWHARFSALWRRYRYSIYTDQW 128
Query: 111 PSI------------GLEVEKVKECSQLFLGVHDFSAFSRVDG--RDTVRSIDRIEVFTL 156
P++ L+ + ++ + LG H +AF R R + + +E +
Sbjct: 129 PNLFADQFSWHYYRFPLQEKTIQAAMEPLLGKHHLAAFHRAGSARRHSWVEVQGVECYRR 188
Query: 157 GPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGL 216
GP++ +EI+A FL+ MVR +V L G G+ S + ++ + K AP +GL
Sbjct: 189 GPMVYLEIQANGFLYGMVRLLVGMLAEVGSGQRSLTDFNDIWVNQRRDLVKY-AAPAKGL 247
Query: 217 LLVDIKYEGIEFPLND 232
L+ + YE FPL D
Sbjct: 248 CLLRVGYE--NFPLPD 261
>sp|Q45557|TRUA_BACSP TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|80158|pir||JT0612 tRNA-pseudouridine synthase I (EC
5.4.99.12) - Bacillus sp. (strain KSM-64)
>gi|289265|gb|AAA73188.1| (M84963) [Bacillus sp. (KSM
64) endo-1,4-beta-glucanase gene, complete cds.], gene
products
Length = 244
Score = 87.8 bits (214), Expect = 1e-16
Identities = 68/217 (31%), Positives = 101/217 (46%), Gaps = 22/217 (10%)
Query: 7 IAYDGTRFHGFQRQPN-LRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVVAITT 65
I YDG R+ G+QR N T++G+I L+ + + RTD GV ALG V +
Sbjct: 8 IQYDGGRYKGWQRLGNGENTIQGKIETVLSEMVGRKIEIIGSGRTDAGVHALGQVANVKL 67
Query: 66 EDEKALNPM--VLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRY----------YLPS 112
+ + + LN L D+ + VP FH R+ AK K Y Y ++
Sbjct: 68 SENFTVKEVKEYLNRYLPHDISVTEVTLVPDRFHSRYNAKDKTYLYKIWNEDYTHPFMRK 127
Query: 113 IGLEVEK------VKECSQLFLGVHDFSAFSRVDG--RDTVRSIDRIEVFTLGPILIIEI 164
L +EK + + SQLF+G HDF+AFS + R+I I + + I +
Sbjct: 128 YSLHIEKKLHIDNMVKASQLFVGEHDFTAFSNAKSKKKTNTRTIHSITIQDNQGFIDIRV 187
Query: 165 EAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGK 201
FL+ MVR++V L GLG E++ +LE K
Sbjct: 188 CGDGFLYNMVRKMVGTLIEVGLGEKEPEQVLTILESK 224
>gb|AAF91402.1|AF266505_1 (AF266505) pseudouridine synthase 3 [Mus musculus]
Length = 481
Score = 83.1 bits (202), Expect = 3e-15
Identities = 77/306 (25%), Positives = 139/306 (45%), Gaps = 50/306 (16%)
Query: 3 VAFKIAYDGTRFHGFQRQPNL-RTVEGEIIKALNNSGIMYS----DFKSASRTDRGVSAL 57
VA KIAY G + GF Q N T+E ++ +AL + ++ S ++ RTD+GVSA
Sbjct: 66 VALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAF 125
Query: 58 GNVVAI-------TTEDEKALN--------------PMVLNARLE-DVWILSAIEVPQDF 95
G V+++ T+ D + N +LN L D+ +L+ V F
Sbjct: 126 GQVISLDLRSQFPTSRDSEDSNLKHEADDLAKEIRYTHILNRVLPADIRVLAWAPVEPSF 185
Query: 96 HPRFWAKSKVYRYYLPSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRDTVRSIDR----- 150
RF + YRY+ P L++ + +Q ++G HDF ++D + V R
Sbjct: 186 SARFSCLERTYRYFFPRADLDIATMNYAAQKYVGTHDFRNLCKMDVANGVIDFQRTILCA 245
Query: 151 -IEVFTLGP----------ILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLE 199
+++ P + E+ ++FL+ VR ++ L L G G E I E+L
Sbjct: 246 QVQLVAQSPGEERRQEPFQLCQFEVIGQAFLYHQVRCMMAILFLIGQGMEKPEIIDELL- 304
Query: 200 GKFEKSKKVPP---APPEGLLLVDIKYEGIEFPLNDKALKKFK-REVEERFRQKIMGAYL 255
+K+ + P A L+L D K+E ++ + D +++F +++ + + ++
Sbjct: 305 -NIQKNPQKPQYSMAVEFPLVLYDCKFENTKW-IYDHEVQEFNVTHLQQLWANHAVKTHM 362
Query: 256 LWDMIQ 261
L+ M+Q
Sbjct: 363 LYSMLQ 368
>sp|P47428|TRUA_MYCGE TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|1361777|pir||B64220 tRNA-pseudouridine synthase I
(EC 5.4.99.12) - Mycoplasma genitalium
>gi|3844778|gb|AAC71401.1| (U39697) pseudouridylate
synthase I (hisT) [Mycoplasma genitalium]
Length = 244
Score = 82.3 bits (200), Expect = 5e-15
Identities = 68/239 (28%), Positives = 115/239 (47%), Gaps = 24/239 (10%)
Query: 7 IAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFK--SASRTDRGVSALGNVVAIT 64
++YDG+ F G+ QPNL T++G + ++ S I+ K + RTD+GV A+ +
Sbjct: 8 VSYDGSYFKGWAIQPNLATIQGLLEQSF--SLIIGRKIKVIGSGRTDKGVHAINQTFHVD 65
Query: 65 TEDEKALNPMV--LNARLEDVWILSAIEVPQD-FHPRFWAKSKVYRYYLP---------- 111
E LN ++ +N ++ I+ + + D FH RF K+KVY Y +
Sbjct: 66 INGEINLNLLIRKINQLIKPHCIVKTLVLVNDSFHARFQVKTKVYEYLINCGNLNPLQFN 125
Query: 112 -----SIGLEVEKVKECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEV-FTLGPILIIEIE 165
+ L++EK+K + LFLG +F +FS D++R+I +I + ++ +
Sbjct: 126 YVWQLNQQLDLEKLKADATLFLGKKNFLSFSSSIHTDSIRTISKITIQKETNQLVRLTFF 185
Query: 166 AKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKYE 224
FL VR IV L +++ E + ++ E +K V AP GL L + YE
Sbjct: 186 GSGFLRSQVRMIVACLVNLNTNKMALETVAKLFEHP-KKGSCVVKAPSCGLYLKTVVYE 243
>gb|AAF23795.1|AF213822_10 (AF213822) tRNA-pseudouridine synthase I [Zymomonas mobilis]
Length = 245
Score = 81.9 bits (199), Expect = 7e-15
Identities = 69/244 (28%), Positives = 114/244 (46%), Gaps = 23/244 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVV 61
R + +DG F G+QR+ + T++G I +A + + ++A RTD GV L
Sbjct: 3 RYRLTVEFDGRNFMGWQRKKHGATIQGSIEEAGASIAGQPTPVQAAGRTDAGVHGLAMTA 62
Query: 62 AI-TTEDEKALNPM-VLNARLE--DVWILSAIEVPQDFHPRFWAKSKVYRYYL----PSI 113
I D M LNARL+ + ILS +V ++H RF + Y Y + P +
Sbjct: 63 HIDVARDFTPFRLMEALNARLKPRPIAILSCEQVDPEWHARFSCIGRRYLYRIINRRPPL 122
Query: 114 GLE------------VEKVKECSQLFLGVHDFSAFSRV--DGRDTVRSIDRIEVFTLGPI 159
LE E + ++ +G+HDF+ F V + V+++D ++V +G
Sbjct: 123 TLEAGRAWHVIKPLDAETMDIAAKRLIGLHDFTTFRSVHCQSKSPVKTLDHLDVRRIGDQ 182
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
+ I A+SFL VR +V L L G G+ + +++ LE + ++ APP+GL
Sbjct: 183 IEITAAARSFLHHQVRSMVGCLVLVGQGQWTADDMTTALEAR-NRAALGFNAPPDGLYFT 241
Query: 220 DIKY 223
Y
Sbjct: 242 QAIY 245
>sp|Q9ZCA3|TRUA_RICPR TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437072|pir||A71648 pseudouridylate synthase I
(truA) RP857 - Rickettsia prowazekii
>gi|3861382|emb|CAA15281.1| (AJ235273) PSEUDOURIDYLATE
SYNTHASE I (truA) [Rickettsia prowazekii]
Length = 253
Score = 80.4 bits (195), Expect = 2e-14
Identities = 68/244 (27%), Positives = 112/244 (45%), Gaps = 23/244 (9%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV- 60
R I Y GT F G+QRQ + +V+ + +A+ + RTD GV A+G V
Sbjct: 11 RYKITIEYLGTHFAGWQRQAGVLSVQQILEEAIYKFSSEQVTLFGSGRTDAGVHAIGQVA 70
Query: 61 ---VAITTEDEKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYL-----PS 112
++ E K + + R DV + + V +FH RF A S+ Y Y + PS
Sbjct: 71 HFDLSKYLEPYKIIKAINYFVRPYDVGVWNCELVSNNFHARFSAISRHYIYRIINRTYPS 130
Query: 113 I-----------GLEVEKVKECSQLFLGVHDFSAF--SRVDGRDTVRSIDRIEVFTLGPI 159
+ L++ +++ + LG HDF++F S + ++++ I +
Sbjct: 131 VIDFNRAWWISSPLDILAMQKAAAYLLGKHDFTSFRSSSCQSKSPIKTLTEINIIKEYEE 190
Query: 160 LIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLV 219
+ + I A SFL MVR IV +L L G E+IK +L+ + ++ P AP GL +
Sbjct: 191 IKLYISAPSFLHYMVRNIVGSLVLVGKNIWQAEQIKNVLDAR-DRKIAGPTAPAFGLYFI 249
Query: 220 DIKY 223
+Y
Sbjct: 250 KAEY 253
>sp|Q50291|TRUA_MYCPN TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|2127630|pir||S62812 tRNA-pseudouridine synthase I
(EC 5.4.99.12) hisT - Mycoplasma pneumoniae (strain ATCC
29342) >gi|2146531|pir||S73961 pseudouridylate synthase
I (EC 5.4.99.12) hisT - Mycoplasma pneumoniae (SGC3)
(ATCC 29342) >gi|1215692|gb|AAC43685.1| (U34795)
hypothetical 28k protein (alkaline endoglucanase);
GT9_orf321 [Mycoplasma pneumoniae]
>gi|1674340|gb|AAB96283.1| (AE000061) pseudouridylate
synthase I [Mycoplasma pneumoniae]
Length = 243
Score = 78.8 bits (191), Expect = 6e-14
Identities = 64/241 (26%), Positives = 111/241 (45%), Gaps = 28/241 (11%)
Query: 7 IAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYS---DFKSASRTDRGVSALGNVVAI 63
+AY+G F G+ +QP TV+G+I + N ++Y + + RTD+GV A+ +
Sbjct: 8 VAYNGAPFRGWAKQPGYHTVQGQIEQ---NLALIYQTPINIYGSGRTDKGVHAINQTFHV 64
Query: 64 TTEDEKALNPMVL---NARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIG------ 114
D+ L ++ D+++ + + V FH RF K+KVY Y + +
Sbjct: 65 DLPDKLPLKQLIAKLNQLNYPDIFVKALVPVAPRFHARFAVKTKVYEYLINTRAFNPAQY 124
Query: 115 ---------LEVEKVKECSQLFLGVHDFSAFSRVDGRDTVRSIDRIEVFT--LGPILIIE 163
L++ +++ + LFLG DF +FS +DT R+I +I V G + +
Sbjct: 125 HTVWQYNHPLDLAQLEADTTLFLGRKDFWSFSTASQKDTQRTISKIIVQQDHTGTVK-LT 183
Query: 164 IEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKY 223
FL +R +V +L L E ++++ + + KV AP GL L + Y
Sbjct: 184 FFGNGFLRNQIRMMVASLVALNNRTLMRETVQQLFDQPQKGGCKV-KAPACGLYLKTVLY 242
Query: 224 E 224
E
Sbjct: 243 E 243
>gi|6321112 Depressed growth-rate protein; Deg1p [Saccharomyces cerevisiae]
>gi|399356|sp|P31115|PUS3_YEAST PSEUDOURIDYLATE SYNTHASE
3 (PSEUDOURIDINE SYNTHASE 3) (DEPRESSED GROWTH-RATE
PROTEIN DEG1) >gi|82981|pir||S14145 depressed
growth-rate protein DEG1 - yeast (Saccharomyces
cerevisiae) >gi|836755|dbj|BAA09239.1| (D50617)
depressed growth-rate protein [Saccharomyces cerevisiae]
>gi|870819|dbj|BAA08034.1| (D44600) DEPRESSED
GROWTH-RATE PROTEIN DEG1 [Saccharomyces cerevisiae]
Length = 442
Score = 76.9 bits (186), Expect = 2e-13
Identities = 74/259 (28%), Positives = 117/259 (44%), Gaps = 35/259 (13%)
Query: 3 VAFKIAYDGTRFHGFQRQPN---LRTVEGEIIKALNNSGIMYS------DFKSASRTDRG 53
+A + AY G ++G Q L TVEG I++A+N ++ S F RTD+G
Sbjct: 94 IALRFAYLGWNYNGLAVQKEYTPLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKG 153
Query: 54 VSALGNVVAITTE----DEKALNPM----------VLNARLEDVWILSAI--EVPQDFHP 97
VSA+ V+++ DE+ +P VLN L D +SA+ P +F
Sbjct: 154 VSAMNQVISLEVRSNLTDEEQRDPTNDSREIPYVHVLNQLLPDDIRISAVCLRPPPNFDA 213
Query: 98 RFWAKSKVYRYYLPSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRDTV----RSIDRIEV 153
RF + Y+Y L +EK+ + + F+G DF F ++DG + R+I ++
Sbjct: 214 RFSCVHRHYKYIFNGKNLNIEKMSKAASYFVGERDFRNFCKLDGSKQITNFKRTIISSKI 273
Query: 154 FTLG-PILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVP--- 209
L ++ +FLW VR ++ L L G L EI L EK+ + P
Sbjct: 274 LPLSETFYCFDLVGSAFLWHQVRCMMAILFLVGQS-LEVPEIVLRLT-DIEKTPQRPVYE 331
Query: 210 PAPPEGLLLVDIKYEGIEF 228
A LLL D K+ +++
Sbjct: 332 MANDIPLLLYDCKFPEMDW 350
>pir||C81355 probable pseudouridylate synthase (EC 4.2.1.70) tRNA-specific
Cj0827 [imported] - Campylobacter jejuni (strain NCTC
11168) >gi|6968273|emb|CAB73092.1| (AL139076) putative
tRNA pseudouridine synthase [Campylobacter jejuni]
Length = 241
Score = 76.5 bits (185), Expect = 3e-13
Identities = 73/251 (29%), Positives = 112/251 (44%), Gaps = 39/251 (15%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNV 60
M++ +YDG+ F G QP+ + V+ + AL++ GI +S ASRTD+GV A V
Sbjct: 2 MKIKIIFSYDGSAFLGSATQPHKKGVQDALSGALSHLGI-FSPLLMASRTDKGVHASYAV 60
Query: 61 VAITTED--------EKALN----PMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRY 108
++ D +K LN P + ++E +V DF RF KS+ YRY
Sbjct: 61 ASVECGDYFTNLEYLQKQLNKFSHPFIHIKKIE--------KVKDDFEVRFDVKSREYRY 112
Query: 109 ------YLPSIG--------LEVEKVKECSQLFLGVHDFSAF--SRVDGRDTVRSIDRIE 152
Y P + ++ K E F+G D F S D + T+R I
Sbjct: 113 IFSHASYSPFMASYVHFYPKFDLGKANELLGFFVGKKDLKFFCKSGGDNKTTLREIFIAR 172
Query: 153 VFTLGPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAP 212
+ I +A FL +R V ++ G++S +E+KE +E K + + + AP
Sbjct: 173 AYAYKDFSIFHFKANGFLRGQIRLSVASVLKVLEGKMSEKELKEQIEAKKQYNHFL--AP 230
Query: 213 PEGLLLVDIKY 223
P GL L I Y
Sbjct: 231 PNGLYLSRICY 241
>pir||A82691 tRNA pseudouridine synthase A XF1373 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9106376|gb|AAF84182.1|AE003968_13 (AE003968) tRNA
pseudouridine synthase A [Xylella fastidiosa]
Length = 257
Score = 76.5 bits (185), Expect = 3e-13
Identities = 68/245 (27%), Positives = 110/245 (44%), Gaps = 23/245 (9%)
Query: 1 MRVAFKIAYDGTRFHGFQRQPNLR-TVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGN 59
MR A + YDG+ F G+Q+ + +V+ + +AL + A RTD GV
Sbjct: 2 MRYALGVEYDGSEFLGWQQLGEMGPSVQATLQQALASVADASVRVVCAGRTDAGVHGQCQ 61
Query: 60 VVAITTEDEKALNPMVLNARLE---DVWILSAIEVPQDFHPRFWAKSKVYRYYL------ 110
VV + + +L V + + +DFH RF A ++ YRY L
Sbjct: 62 VVHFDSAVTRPPRAWILGTTTRLPSSVAVRWCVPTSEDFHARFSACARRYRYRLLNRQVR 121
Query: 111 PSIG----------LEVEKVKECSQLFLGVHDFSAF--SRVDGRDTVRSIDRIEVFTLGP 158
P++ L+ + + +Q+ LG +DFSAF ++ R + I V
Sbjct: 122 PALQHQFLSWERHPLDAQAMHVAAQMLLGENDFSAFRSAQCQALHARRELQAISVRRYAE 181
Query: 159 ILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLL 218
++ I ++A +FL MVR IV +L + G G E I E+L G+ +++ P A GL+
Sbjct: 182 VIEICVQANAFLHHMVRNIVGSLLMVGTGERPMEWIAELLAGR-DRTMAGPTASARGLVF 240
Query: 219 VDIKY 223
V Y
Sbjct: 241 VGPLY 245
>sp|Q09524|YQN3_CAEEL PROBABLE PSEUDOURIDYLATE SYNTHASE E02H1.3 (PSEUDOURIDINE SYNTHASE)
>gi|7498308|pir||T20413 hypothetical protein E02H1.3 -
Caenorhabditis elegans >gi|3875428|emb|CAA87378.1|
(Z47075) similar to Yeast DEG-1 protein (Swiss Prot
accession number P31115), contains similarity to Pfam
domain: PF01416 (tRNA pseudouridine synthase),
Score=163.2, E-value=1.5e-45, N=1~cDNA EST yk101h10.5
comes from this gene~cDNA EST yk101h10.3 come>
Length = 402
Score = 75.7 bits (183), Expect = 5e-13
Identities = 69/284 (24%), Positives = 123/284 (43%), Gaps = 49/284 (17%)
Query: 2 RVAFKIAYDGTRFHGFQRQPNLR-TVEGEIIKALNNSGIMYS----DFKSASRTDRGVSA 56
++A + Y G G +QP+ + TVE I++AL + ++ DF RTD+GVSA
Sbjct: 31 KIAIQFFYLGWEHDGLVQQPHTQNTVENHIMQALIKTHLIEDWTKCDFSRCGRTDKGVSA 90
Query: 57 LGNVVAI----------------TTEDEKALNPMVLNARLEDVWILSAI----------- 89
A+ +T++ + ++ L V +L+ +
Sbjct: 91 FKQTAAMVVRSLCPGDSGVFWSDSTQEHQKVDYKASGEELPYVKMLNGVLPKTIRVFAWA 150
Query: 90 EVPQDFHPRFWAKSKVYRYYLPSIGLEVEKVKECSQLFLGVHDFSAFSRVDGRDT----- 144
V Q F+ RF + Y+Y L +EK+++ ++L +G HDFS F ++D +
Sbjct: 151 PVAQTFNARFDCNRRTYKYSFAKADLNLEKMRQGAELLVGEHDFSNFCQIDMNEKRLLQS 210
Query: 145 -VRSIDRIEVFTLG--------PILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIK 195
VR + ++V + ++ + + FLW M+R IV L+ G I
Sbjct: 211 YVRKVYEVKVEQVSTHPENDMYSMVELTVSGSGFLWHMIRYIVTILQEIGRENEQPSLIS 270
Query: 196 EMLE-GKFEKSKKVPPAPPEGLLLVDIKY--EGIEFPLNDKALK 236
++L+ K+ + A L L D Y E +E+ ++D LK
Sbjct: 271 QLLDLKKYPSRPQYTLASDTPLCLFDCGYKSEDVEWKVHDYTLK 314
>pir||S69220 pseudouridine synthase 1 - yeast (Saccharomyces cerevisiae)
(fragments)
Length = 270
Score = 73.0 bits (176), Expect = 4e-12
Identities = 75/274 (27%), Positives = 122/274 (44%), Gaps = 40/274 (14%)
Query: 3 VAFKIAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSD---------FKSASRTDRG 53
VA + Y GT +HG Q P T+E + KA +G + D F A+RTD+G
Sbjct: 1 VAVMVGYCGTGYHGMQYNPPNPTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKG 60
Query: 54 VSALGNVVAITTEDEKALNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPS 112
V A GN++++ E +N +L E + + V + F R S+ Y Y LP+
Sbjct: 61 VHAGGNLISLKMIIEDPDIKQKINEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPT 120
Query: 113 IGLEVEKVKECSQLFLGVHDFSAFSRVDGRD-----TVRSIDRIEV---FTLGPI----L 160
L K G H+F F+ G+D +R + I+V F +G +
Sbjct: 121 YSLIGPKPG-------GAHNFHNFTL--GKDFKEPSAIRFMKDIKVSDPFVIGDAQTEWI 171
Query: 161 IIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVD 220
I+I +SF+ +R++V L + R C ++ + + ++ +P AP GLLL
Sbjct: 172 SIKIHGQSFMLHQIRKMVSMATL--ITRCGC-PVERISQAYGQQKINIPKAPALGLLLEA 228
Query: 221 IKYEGIEFPLNDKALK-----KFKREVEERFRQK 249
+EG L K K++ EV ++F+ K
Sbjct: 229 PVFEGYNKRLEQFGYKAIDFSKYQDEV-DKFKMK 261
>sp|O06322|TRUA_MYCTU TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437075|pir||D70565 probable pseudouridylate
synthase - Mycobacterium tuberculosis (strain H37RV)
>gi|2104378|emb|CAB08721.1| (Z95390) truA [Mycobacterium
tuberculosis]
Length = 256
Score = 72.2 bits (174), Expect = 6e-12
Identities = 65/235 (27%), Positives = 98/235 (41%), Gaps = 36/235 (15%)
Query: 24 RTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVVAITTEDEKALNP---------- 73
RTV G++ AL ++A RTD GV A G V + + N
Sbjct: 4 RTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHVGDP 63
Query: 74 --MVLNARL-----EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSIG------------ 114
+ L RL DV IL P F RF A + Y Y L +
Sbjct: 64 EFLPLLRRLGRFLPADVRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQARYIT 123
Query: 115 -----LEVEKVKECSQLFLGVHDFSAFSRV-DGRDTVRSIDRIEVFTLGPILIIEIEAKS 168
L+++ + S+ +G+HDF+AF R +G T+R + R++ G ++ + A +
Sbjct: 124 AWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVTADA 183
Query: 169 FLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKY 223
F W MVR +V AL G R + +E+L +S AP GL L+ + Y
Sbjct: 184 FCWSMVRSLVGALLAVGEHRRATTWCRELLTAT-GRSSDFAVAPAHGLTLIQVDY 237
>gb|AAF55785.1| (AE003731) CG4159 gene product [Drosophila melanogaster]
Length = 410
Score = 70.6 bits (170), Expect = 2e-11
Identities = 72/295 (24%), Positives = 124/295 (41%), Gaps = 64/295 (21%)
Query: 4 AFKIAYDGTRFHGFQRQPNLRTVEGEIIKAL---------NNSGIMYSDFKSASRTDRGV 54
A ++Y G ++G QR P ++T+E E+ KA+ + I S F+ A+RTD+GV
Sbjct: 73 AILLSYCGANYYGMQRNPGMQTIEEELFKAMLKHKWITEDSFEQIQISCFQRAARTDKGV 132
Query: 55 SALGNVVAITTEDEKALNPMVLNARL-EDVWILSAIEVPQDFHPRFWAKSKVYRYYLPSI 113
SA V ++ +E L+ NA L + + + V + F+ + ++ Y Y LP++
Sbjct: 133 SAARQVCSVKLPEE--LDLEAFNADLPQQIRLFGVERVTKGFNAKDQCNARTYTYTLPTV 190
Query: 114 GLE-------------------VEKVKECSQLFLGVHDFSAFS---------------RV 139
++KVKE +L+ G +F F+
Sbjct: 191 AFAPFEEKVDDVHDTFRISPELLQKVKETLKLYEGTKNFHNFTSKKSFLDPSSKRFIMSF 250
Query: 140 DGRDTVRSIDRIEVFTLGPILIIEIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLE 199
+ RS IE TL +++ +SF+ +R++V G + ++ L
Sbjct: 251 TSSEPFRSPQDIEFVTL------KVKGQSFMLHQIRKMVGLAIAIVRGNTTAATLERALT 304
Query: 200 GKFEKSKKVPPAPPEGLLLVDIKYE---------GIEFPLNDKALKKFKREVEER 245
E+ +P AP GL+L + YE GI PL +A + +E ER
Sbjct: 305 ---EERLDLPMAPGLGLVLDTVHYERYNDRYGKDGIHNPLTWQAQEAQVQEFIER 356
>sp|Q9ZKC0|TRUA_HELPJ TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437071|pir||F71858 pseudouridylate synthase I -
Helicobacter pylori (strain J99)
>gi|4155610|gb|AAD06601.1| (AE001530) PSEUDOURIDYLATE
SYNTHASE I [Helicobacter pylori J99]
Length = 242
Score = 68.7 bits (165), Expect = 7e-11
Identities = 70/241 (29%), Positives = 109/241 (45%), Gaps = 30/241 (12%)
Query: 7 IAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVVAITTE 66
+AYDG F G+ +QPN V+ +I ALN GI S +A RTD+GV A N A++
Sbjct: 8 VAYDGAYFLGYAKQPNKLGVQDKIESALNALGIK-SGVIAAGRTDKGVHA--NNQALSFH 64
Query: 67 DEKALNPM----VLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYL----------PS 112
K N L +L +L +E ++FH RF A+ + YRY L P
Sbjct: 65 APKHWNAAKLFHYLAPKLAPHIVLKKLE-EKNFHARFDAQKRAYRYLLTKNLKTPFLAPY 123
Query: 113 IGL----EVEKVKECSQLFLGVHDFSAFSRVDGRDT-----VRSIDRIEVFTLG-PILII 162
I ++ + + F+G HDFS F + G T + + + F +G ++
Sbjct: 124 IACGDYGSLDALNIALKQFIGKHDFSMFKKEGGATTNPNRIIFNALAYKTFIMGHECVVF 183
Query: 163 EIEAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIK 222
+I +FL VR I++A L +++ EI+ + K+ P GL L +
Sbjct: 184 KIIGDAFLRSSVRLIIQACVQYSLEKITLAEIQAQIHNL--KATIRTPIMANGLYLHRVY 241
Query: 223 Y 223
Y
Sbjct: 242 Y 242
>sp|P56144|TRUA_HELPY TRNA PSEUDOURIDINE SYNTHASE A (PSEUDOURIDYLATE SYNTHASE I)
(PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE)
>gi|7437070|pir||A64565 pseudouridylate synthase I -
Helicobacter pylori (strain 26695)
>gi|2313463|gb|AAD07429.1| (AE000553) pseudouridylate
synthase I (hisT) [Helicobacter pylori 26695]
Length = 242
Score = 68.3 bits (164), Expect = 9e-11
Identities = 69/239 (28%), Positives = 107/239 (43%), Gaps = 26/239 (10%)
Query: 7 IAYDGTRFHGFQRQPNLRTVEGEIIKALNNSGIMYSDFKSASRTDRGVSALGNVVAITTE 66
IAYDG F G+ +QPN V+ +I ALN GI S +A RTD+GV A V++
Sbjct: 8 IAYDGAYFLGYAKQPNKLGVQDKIEDALNALGIK-SVVIAAGRTDKGVHANNQVLSFHAP 66
Query: 67 D--EKALNPMVLNARLEDVWILSAIEVPQDFHPRFWAKSKVYRYYL----------PSIG 114
A L +L +L +E ++FH RF A+ + YRY L P I
Sbjct: 67 KHWNAAKLFYYLAPKLAPHIVLKKLE-EKNFHARFDAQKRAYRYLLTKNLKTPFLAPYIA 125
Query: 115 L----EVEKVKECSQLFLGVHDFSAFSRVDG-----RDTVRSIDRIEVFTLG-PILIIEI 164
++ + + F G HDFS F + G + T+ + + F +G ++ +I
Sbjct: 126 CGDYGSLDALNTALKQFTGKHDFSMFKKEGGATTNPKRTIFNAFAYKTFIIGHECVVFKI 185
Query: 165 EAKSFLWEMVRRIVKALELCGLGRLSCEEIKEMLEGKFEKSKKVPPAPPEGLLLVDIKY 223
+FL VR I++A L +++ EI+ + K+ P GL L + Y
Sbjct: 186 IGDAFLRSSVRLIIQACVQYSLEKITLAEIQAQIHNL--KATIRTPIMANGLYLHRVYY 242
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.141 0.411
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93077806
Number of Sequences: 2977
Number of extensions: 3649941
Number of successful extensions: 9120
Number of sequences better than 1.0e-10: 45
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 42
Number of HSP's that attempted gapping in prelim test: 8999
Number of HSP's gapped (non-prelim): 46
length of query: 263
length of database: 189,106,746
effective HSP length: 54
effective length of query: 209
effective length of database: 156,788,448
effective search space: 32768785632
effective search space used: 32768785632
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 164 (68.3 bits)