BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1702 (nirJ-like) DE:HEME BIOSYNTHESIS PROTEIN related
(441 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75076 heme biosynthesis protein related PAB1702 - Pyrococc... 926 0.0
pir||E72327 astB/chuR-related protein - Thermotoga maritima (str... 113 6e-24
gi|11499591 heme biosynthesis protein (nirJ-2) [Archaeoglobus fu... 109 5e-23
pir||F71132 probable nitrite reductase - Pyrococcus horikoshii >... 101 2e-20
pir||B70059 conserved hypothetical protein ywiA - Bacillus subti... 92 2e-17
pir||D69105 coenzyme PQQ synthesis protein - Methanobacterium th... 84 3e-15
pir||H70640 probable pqqE protein - Mycobacterium tuberculosis (... 81 3e-14
gb|AAK42465.1| Cofactor biosynthesis protein [Sulfolobus solfata... 80 4e-14
gb|AAG19560.1| (AE005046) coenzyme PQQ synthesis protein; PqqE [... 79 1e-13
pir||E72268 astB/chuR-related protein - Thermotoga maritima (str... 79 1e-13
gb|AAK42036.1| Coenzyme PQQ synthesis protein E (pqqE-5) [Sulfol... 78 3e-13
gb|AAK41779.1| Heme biosynthesis related protein [Sulfolobus sol... 75 2e-12
gb|AAK41844.1| Coenzyme PQQ synthesis protein E (pqqE-2) [Sulfol... 73 7e-12
emb|CAC12477.1| (AL445067) heme biosynthesis protein (NirJ) rela... 71 2e-11
pir||C83581 heme d1 biosynthesis protein NirJ PA0511 [imported] ... 71 3e-11
pir||D75071 tungsten-containing aldehyde ferredoxin oxidoreducta... 70 6e-11
>pir||C75076 heme biosynthesis protein related PAB1702 - Pyrococcus abyssi
(strain Orsay) >gi|5458424|emb|CAB49912.1| (AJ248286)
HEME BIOSYNTHESIS PROTEIN related [Pyrococcus abyssi]
Length = 441
Score = 926 bits (2366), Expect = 0.0
Identities = 441/441 (100%), Positives = 441/441 (100%)
Query: 1 MSKWMCSLCYWSYTSVYLIIFFTRGKIKMEGTLFLPNPQLLWEIKSNKAVIYLPDKEIGL 60
MSKWMCSLCYWSYTSVYLIIFFTRGKIKMEGTLFLPNPQLLWEIKSNKAVIYLPDKEIGL
Sbjct: 1 MSKWMCSLCYWSYTSVYLIIFFTRGKIKMEGTLFLPNPQLLWEIKSNKAVIYLPDKEIGL 60
Query: 61 DREGTDIFFQIIRRQPIIATTTEQEEFIEELMEYNVIMPYDPINPKFFPPGAIAVEITHR 120
DREGTDIFFQIIRRQPIIATTTEQEEFIEELMEYNVIMPYDPINPKFFPPGAIAVEITHR
Sbjct: 61 DREGTDIFFQIIRRQPIIATTTEQEEFIEELMEYNVIMPYDPINPKFFPPGAIAVEITHR 120
Query: 121 CNLKCEHCYVGTRNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTLHPNFEKILEIIH 180
CNLKCEHCYVGTRNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTLHPNFEKILEIIH
Sbjct: 121 CNLKCEHCYVGTRNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTLHPNFEKILEIIH 180
Query: 181 KSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRKSVKFEEIINNI 240
KSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRKSVKFEEIINNI
Sbjct: 181 KSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRKSVKFEEIINNI 240
Query: 241 KKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYPVGNARSHANIFPSS 300
KKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYPVGNARSHANIFPSS
Sbjct: 241 KKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYPVGNARSHANIFPSS 300
Query: 301 TEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYYLCSAGVTQVTIDAEG 360
TEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYYLCSAGVTQVTIDAEG
Sbjct: 301 TEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYYLCSAGVTQVTIDAEG 360
Query: 361 NITPCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEFRLMWPENESCARCGLVCAKC 420
NITPCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEFRLMWPENESCARCGLVCAKC
Sbjct: 361 NITPCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEFRLMWPENESCARCGLVCAKC 420
Query: 421 PATVLGIKGDIRKPDPRCPLQ 441
PATVLGIKGDIRKPDPRCPLQ
Sbjct: 421 PATVLGIKGDIRKPDPRCPLQ 441
>pir||E72327 astB/chuR-related protein - Thermotoga maritima (strain MSB8)
>gi|4981357|gb|AAD35907.1|AE001750_1 (AE001750)
astB/chuR-related protein [Thermotoga maritima]
Length = 323
Score = 113 bits (279), Expect = 6e-24
Identities = 86/318 (27%), Positives = 141/318 (44%), Gaps = 41/318 (12%)
Query: 109 PPGAIAVEITHRCNLKCEHCYVGT-RNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPT 167
PP + E+T RCN +C+HCY + + LS E IK+LI DM++LG + L I GGEP
Sbjct: 4 PPFIVEYELTLRCNFRCKHCYCEAGKPHLEELSFEEIKELILDMKELGTWALDIVGGEPL 63
Query: 168 LHPNFEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGP-KEIHEK 226
LHP IL + I TNG+ E ++K K + +S++GP E ++
Sbjct: 64 LHPQILDILAFGKEVGQRLMINTNGSLATKEMVQK-IKKANPDVLIGVSLEGPDPETNDF 122
Query: 227 VRKSVKFEEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYP 286
VR + F+ + IK + G++ I ++ + ++ ++K +L ++A V P
Sbjct: 123 VRGTGNFKRAVQGIKNFVDEGFQVTILNVINKRNWRKFEDMVKLALELGVNALYVDRFIP 182
Query: 287 VGNARSHANIFPSSTEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYYL 346
VG HA + E EW R+ LG I+ +Y+
Sbjct: 183 VGRGMIHARELDMNPE---------EW-------RVAIKHVLG----VIENYKNHLTFYV 222
Query: 347 --------CSAGVTQVTIDAEGNITPCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLW 398
CSAG+T ++ A+G + PC + ++ GN+ E+WH +
Sbjct: 223 EESISGKPCSAGITHASVLADGTVVPCGHFRY---RKEFYMGNVREKKFSEIWHEYTPI- 278
Query: 399 EFRLMWPENESCARCGLV 416
P SC +C ++
Sbjct: 279 ------PSPASCQQCPIL 290
>gi|11499591 heme biosynthesis protein (nirJ-2) [Archaeoglobus fulgidus]
>gi|7483317|pir||H69500 heme biosynthesis protein
(nirJ-2) homolog - Archaeoglobus fulgidus
>gi|2648528|gb|AAB89245.1| (AE000964) heme biosynthesis
protein (nirJ-2) [Archaeoglobus fulgidus]
Length = 468
Score = 109 bits (271), Expect = 5e-23
Identities = 99/365 (27%), Positives = 157/365 (42%), Gaps = 51/365 (13%)
Query: 106 KFFPPGA---IAVEITHRCNLKCEHCYVGTRNNPAT--LSLEVIKKLIHDMEKLGCYQLA 160
K F PGA + +IT+ CNL+C+HCY T P L+ + + I +++LG +A
Sbjct: 106 KPFVPGAPFQVVWDITYSCNLRCKHCYA-TAGKPLQDELTTDEALETIDKLDRLGVTIIA 164
Query: 161 IGGGEPTLHPNFEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGP 220
GGEP + + ++ + I+ I TNGT E ++ K+ V IS+DG
Sbjct: 165 FSGGEPLVRRDIFELTRYAAEKGIYVAIATNGTLITEEIAKRM--KENGVGYVQISLDGM 222
Query: 221 KEIHEKVRKSVK--FEEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISA 278
KE HE R ++ F++ + I+ + G + TV+ Y +PK+++ + L ++
Sbjct: 223 KETHEAFR-GIRGCFDKTVEGIRNAVKTGLFVNVSMTVTRYNYHEVPKVVELCEKLGVN- 280
Query: 279 WAVKMEY-PVGNARS--HANIFPSSTEFVKMTSEIWEWWEESNLK-------------RI 322
W + + P G R A+I P E E+ +W E N R+
Sbjct: 281 WFMHYNFIPTGRGREIVEADITPEQRE------ELLKWLYEKNYSSNISLLSTAPQFARV 334
Query: 323 EF-----VEDLGYFPPAIKKPAKKRAYYL--CSAGVTQVTIDAEGNITPCTLIQITDGST 375
+ ++ + K+ A ++ C AG I A G+I PC +
Sbjct: 335 ALQCQGEIVPTHFYNVNAGERLKELAEFIGGCGAGRFYFAIKANGDIQPCVFFPLK---- 390
Query: 376 KYIAGNLYYDSLIEVWHTSNVLWEFRLMWPENESCARCGL--VCAKCPATVLGIKGDIRK 433
GN+ D L E+W ++V + R E C CG VC C A GD K
Sbjct: 391 ---VGNVREDDLEELWLHNHVFEDLRDK-DILEGCGSCGYRYVCGGCRARAYNYFGDYTK 446
Query: 434 PDPRC 438
PDP C
Sbjct: 447 PDPGC 451
>pir||F71132 probable nitrite reductase - Pyrococcus horikoshii
>gi|3257237|dbj|BAA29920.1| (AP000003) 552aa long
hypothetical nitrite reductase [Pyrococcus horikoshii]
Length = 552
Score = 101 bits (249), Expect = 2e-20
Identities = 88/374 (23%), Positives = 152/374 (40%), Gaps = 37/374 (9%)
Query: 88 IEELMEYNVIMPYDPINPKFFPPGAIAVEITHRCNLKCEHCYV-GTRNNPATLSLEVIKK 146
+E + +Y V +P K P I T+ CN +C+HCY + P+ LSLE
Sbjct: 131 LEGIAKYGVTVPQ-----KLAAPFLIVWNFTNMCNFRCKHCYQRADKPLPSELSLEEKLM 185
Query: 147 LIHDMEKLGCYQLAIGGGEPTLHPNFEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANK 206
L+ +++ G +A+ GGEPT+HP+F +I++ + I + TNG T + EK
Sbjct: 186 LVDQLDRAGVAAVALSGGEPTIHPHFLRIVKELSSRGIHTSVATNGWTFADK--EKLEEA 243
Query: 207 KKRSFE-VTISIDGPK-EIHEKVRK-SVKFEEIINNIKKLAELGWKPQIQTTVSLKTYKY 263
K + V IS+D K E H++ R +E I ++ E+G + + +TY+
Sbjct: 244 VKAGLKYVEISVDSAKPEKHDEFRGIPGAWERAIKALENAVEIGVSHGMAVIMDKETYQE 303
Query: 264 IPKLLKELKDLPISAWAVKMEYPVGNARSHANIFPSSTEFVKMTSEIWEWWEESNLK--- 320
+ +L + + + P G A + + E + E++ ++ L+
Sbjct: 304 MDDILDLAESIGVKRVIFFNLVPTGRAEDMVKVDLTPEEREEFLKELYHQMKKRKLEILT 363
Query: 321 ------RIEFVEDLG------YFPPAIKKPAKKRAYYL--CSAGVTQVTIDAEGNITPCT 366
R+ +E G +F K A ++ C AG I+ +G + PC
Sbjct: 364 TAPQYARVTLIESQGKNVSPAHFYVGQNTAVKTLAEFIGGCGAGRIYAGIEPDGTVVPCV 423
Query: 367 LIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEFRLMWPENESCARCGL--VCAKCPATV 424
+ + GN+ E+W S + R C +C +C C A
Sbjct: 424 FLPLP-------VGNVRVKPFKEIWENSRIFNLLRDRDNFTGQCKKCPYRNICGGCRARA 476
Query: 425 LGIKGDIRKPDPRC 438
D+ DP C
Sbjct: 477 YHYTLDLLGDDPGC 490
>pir||B70059 conserved hypothetical protein ywiA - Bacillus subtilis
>gi|1565254|emb|CAB02509.1| (Z80360) Unknown [Bacillus
subtilis] >gi|2636273|emb|CAB15764.1| (Z99123) similar
to hypothetical proteins [Bacillus subtilis]
Length = 448
Score = 91.7 bits (224), Expect = 2e-17
Identities = 80/293 (27%), Positives = 132/293 (44%), Gaps = 32/293 (10%)
Query: 116 EITHRCNLKCEHCYVGTRNNP-ATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTLHPNFEK 174
E+THRCNLKC HCY+ + T+S+E KK + G I GGE +HPN +
Sbjct: 124 ELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDNGVLTCEITGGEIFVHPNANE 183
Query: 175 ILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDG-PKEIHEKVR-KSVK 232
IL+ + K ++TNGT E LE K++ V IS+D E+H+ R +
Sbjct: 184 ILDYVCKKFKKVAVLTNGTLMRKESLELLKTYKQKII-VGISLDSVNSEVHDSFRGRKGS 242
Query: 233 FEEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYPVGNARS 292
F + IK L++ G ++ +V K I + ++++DL A++ G R
Sbjct: 243 FAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKVRDLGAKAFSYNWVDDFGRGR- 301
Query: 293 HANIFPSSTEFVKMTSEIWEWWEESNLKRIEF----VEDLGYFPPAIKKPAKKRAYYLCS 348
+ V T + E + K +E+ +++ P I K+ A C
Sbjct: 302 ---------DIVHPTKD-----AEQHRKFMEYEQHVIDEFKDLIPIIPYERKRAAN--CG 345
Query: 349 AGVTQVTIDAEGNITPCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNV--LWE 399
AG + I G + PC L ++ GN+++DS ++++ V LW+
Sbjct: 346 AGWKSIVISPFGEVRPCALF-----PKEFSLGNIFHDSYESIFNSPLVHKLWQ 393
>pir||D69105 coenzyme PQQ synthesis protein - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622916|gb|AAB86251.1| (AE000933) coenzyme PQQ
synthesis protein [Methanobacterium thermoautotrophicum]
Length = 353
Score = 83.9 bits (204), Expect = 3e-15
Identities = 77/345 (22%), Positives = 138/345 (39%), Gaps = 40/345 (11%)
Query: 123 LKCEHCYVGTRNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTLHPNFEKILEIIHKS 182
+KC+HCY + ++I + G LA GGEP++HP + + H++
Sbjct: 1 MKCKHCYEYAGRKSCEMKPSEGLRVIDTLADAGVTSLAFSGGEPSIHPTIIEYISRSHEN 60
Query: 183 KIFAHIVTNG-TTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVR-KSVKFEEIINNI 240
++ + TNG + + K+ + + +++I P E+H++ R +E +
Sbjct: 61 GMYTAMATNGYIFSDMDACSKFVDAGLQFVQISIDSIRP-EVHDRFRGVDGSWERACEAV 119
Query: 241 KKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYPVGNARSHANIFPSS 300
K +E ++ TTV+ + ++ + + +L + + P G + + S
Sbjct: 120 KNFSEYDILVEVATTVTSENCMHLAGMGEFFHELGADWFMLYNFIPAGRGNENQELDISP 179
Query: 301 TEFVKMTSEIWEWWEESNL-KRIEFVEDLGYFPPAI------KKPAKKRAYYL------- 346
E ++ + SNL ++I+ + F P KK Y
Sbjct: 180 GERYRVLCDA----HHSNLQEKIQILSTAPQFAPIAAGLSHDKKIIPTHFYNPEYTEETL 235
Query: 347 --------CSAGVTQVTIDAEGNITPCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLW 398
C AG ++I+ +G+I PC + GN+ DS ++W T+ VL
Sbjct: 236 QLAEFIGGCGAGRFYLSIEPDGDIYPCVFFPHREDMR---VGNILEDSFEDLWKTNKVLK 292
Query: 399 EFRLMWPENESCARCG-----LVCAKCPATVLGIKGDIRKPDPRC 438
R +E CG +C C A L GDIR PDP C
Sbjct: 293 TLR---DRDELTGHCGSCESRYICGGCRARALSYLGDIRSPDPGC 334
>pir||H70640 probable pqqE protein - Mycobacterium tuberculosis (strain H37RV)
>gi|1806159|emb|CAB06456.1| (Z84395) pqqE [Mycobacterium
tuberculosis]
Length = 391
Score = 80.8 bits (196), Expect = 3e-14
Identities = 77/339 (22%), Positives = 143/339 (41%), Gaps = 26/339 (7%)
Query: 110 PGAIAVEITHRCNLKCEHCYVGT-RNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTL 168
P + E+T+ CNL C HC + + +P LS K +I ++E++ + + IGGGEPT+
Sbjct: 19 PICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDELERMQVFYVNIGGGEPTV 78
Query: 169 HPNFEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDG-PKEIHEKV 227
P+F ++++ + TNG E + A +V IS+DG E+++ +
Sbjct: 79 RPDFWELVDYATAHHVGVKFSTNGVRITPEVATRLA--ATDYVDVQISLDGATAEVNDAI 136
Query: 228 RKSVKFEEIINNIKKLAELGWK-PQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYP 286
R + F+ + ++ LA G+ +I ++ + + + + ++
Sbjct: 137 RGTGSFDMAVRALQNLAAAGFAGVKISVVITRRNVAQLDEFATLASRYGATLRITRLRPS 196
Query: 287 VGNARSHANIFPSSTEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYYL 346
A++ P++ + V++ W S +R+ + + P + + +
Sbjct: 197 GRGTDVWADLHPTADQQVQLYD-----WLVSKGERVLTGDSFFHLAP-LGQSGALAGLNM 250
Query: 347 CSAGVTQVTIDAEGNITPCTLIQITDGSTKYIAGNLYYDSLIE-VWHTSNVLWEFRLMWP 405
C AG ID G++ C I D ++AGN+ D + VW S++ E R
Sbjct: 251 CGAGRVVCLIDPVGDVYACP-FAIHD---HFLAGNVLSDGGFQNVWKNSSLFRELR---- 302
Query: 406 ENESCARCGLV-----C-AKCPATVLGIKGDIRKPDPRC 438
E +S CG C C A + PDP C
Sbjct: 303 EPQSAGACGSCGHYDSCRGGCMAAKFFTGLPLDGPDPEC 341
>gb|AAK42465.1| Cofactor biosynthesis protein [Sulfolobus solfataricus]
Length = 344
Score = 80.4 bits (195), Expect = 4e-14
Identities = 75/310 (24%), Positives = 138/310 (44%), Gaps = 43/310 (13%)
Query: 116 EITHRCNLKCEHCYVGTRNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTLHPNFEKI 175
++ + CNL+C C R + LSLE ++ +EK G + GGEP L + E I
Sbjct: 31 KLLYTCNLRCRMCPFWRRKDEKLLSLEEEVLMLKSLEKAGVLFMGFEGGEPLLRRDLEDI 90
Query: 176 LEIIHKSKIFAHIVTNG------TTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRK 229
LE +K + + +VTNG ++S YL+ + +SIDG E+H+K+R
Sbjct: 91 LEESYK-RFYTSLVTNGWLLKDRAKSISNYLD----------YLFVSIDGIGEVHDKIRG 139
Query: 230 -SVKFEEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYPVG 288
S FE + IK+ A I T++ + + +++ + L ++ ++++EY
Sbjct: 140 ISGAFERAVEGIKE-ARKYLPVAISFTITKENMDQVKDVIELARKLNVNI-SIQVEYDYS 197
Query: 289 NARSHANIFPSSTEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYYLCS 348
A + + +++ E+ + + +E YF AI K + C
Sbjct: 198 TAEKLSPDKRRLYDALRLIIEL-------KKRGYQIIESSDYF-EAIIKSWYNGIPWRCK 249
Query: 349 AGVTQVTIDAEGNIT-PCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEFRLMWPEN 407
+T + ID +G I PC ++ G+ K ++ ++++W++ W +
Sbjct: 250 PWLT-INIDPQGRIVLPCYVLNEYSGTDK-----VWEVDIVKLWNS--------YPWEKY 295
Query: 408 ESCARCGLVC 417
ESC C L C
Sbjct: 296 ESCNACALAC 305
>gb|AAG19560.1| (AE005046) coenzyme PQQ synthesis protein; PqqE [Halobacterium sp.
NRC-1]
Length = 356
Score = 78.8 bits (191), Expect = 1e-13
Identities = 78/336 (23%), Positives = 130/336 (38%), Gaps = 14/336 (4%)
Query: 110 PGAIAVEITHRCNLKCEHCYVGTR--NNPATLSLEVIKKLIHDMEKLGCYQLAI-GGGEP 166
P + E+T C L C+HC R +P+ LS K+L+ D G QL + GG+P
Sbjct: 10 PVVLVWEVTQACALACDHCRASARPQRHPSELSTAEGKQLLTDAAAFGDGQLVVLSGGDP 69
Query: 167 TLHPNFEKILEIIHKSKIFAHIVTNGTTNLSEY-LEKYANKKKRSFEVTISIDGPKEIHE 225
P+ +++E + + +GT +L+ +E A+ F V+I P E
Sbjct: 70 LARPDTVELVEHGTDCGLRMTVTPSGTASLTPTAIEALADAGVAQFAVSIDGATPTTHDE 129
Query: 226 KVRKSVKFEEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEY 285
++ FE + + + ELG Q+ TTV T + +P + + +L ++ W+V
Sbjct: 130 FRGEAGSFERTLRAARAIRELGVPLQVNTTVCADTVEALPAIRDLVAELGVALWSVFFLV 189
Query: 286 PVGNARSHANIFPSSTEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYY 345
PVG R+ + P+ E E+ W + + R E P ++ +R
Sbjct: 190 PVGRGRALDPVSPARAE------EVMAWLD--GVARSEAFGVKTTEAPMYRRVRAQRGGD 241
Query: 346 LCSAGVTQVTIDAEGNITPCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEFRLMWP 405
VT T G AGN+ S+++ + S + R
Sbjct: 242 GAPQRRAGVTAGDGFAFVSHTGAVYPSGFLPSSAGNVRERSVVDSYRNSELFQRLRDTEA 301
Query: 406 ENESCARCGL--VCAKCPATVLGIKGDIRKPDPRCP 439
C C VC + G+ DP CP
Sbjct: 302 LTGKCGACSFRTVCGGSRSRAYATTGEPMASDPLCP 337
>pir||E72268 astB/chuR-related protein - Thermotoga maritima (strain MSB8)
>gi|4981877|gb|AAD36391.1|AE001786_4 (AE001786)
astB/chuR-related protein [Thermotoga maritima]
Length = 463
Score = 78.8 bits (191), Expect = 1e-13
Identities = 94/370 (25%), Positives = 148/370 (39%), Gaps = 44/370 (11%)
Query: 52 YLPDKEIGLDREGTDIFFQIIRRQPIIATTTEQEEFIEEL--MEYNVIMPYDPINPKFFP 109
YL DK + D D QI R+ T+ + IEEL ++ N ++ K
Sbjct: 22 YLLDK-VNFDFYKLDDSIQITRKASSYLVRTKAKNVIEELEKLKKNTLISLKRKLVKDND 80
Query: 110 PGAIAVEITHRCNLKCEHCYV--GTRNNPATLSLEV-IKKLIHDMEKLGCYQLAIGGGEP 166
+A+ ++ C LKC +CY GT NP + V ++ L + + + I GGEP
Sbjct: 81 ILRLALNVSQVCRLKCRYCYANAGTYGNPGFMKRNVLVRTLDFFLSNYNVHNIHIFGGEP 140
Query: 167 TLHPNFEKILEIIH-----KSKIFAHIVTNGTTNL-------SEYLEKYAN-KKKRSFEV 213
L N E I E+ +K F ++ T+L + +++ Y + K K +
Sbjct: 141 LL--NIEMIEEMFRILTSKYAKKFNNLRITVATSLFVSEETINRFIKIYESYKDKFQIYM 198
Query: 214 TISIDGPKEIHEKVR-----KSVKFEEIINNIKKLAELGWKPQIQTTVS----------L 258
+S+DGPKEI + R KS F+ I NNI L + G+ + T + L
Sbjct: 199 VVSLDGPKEIQDNTRPSLDSKSSSFDRIKNNINLLKQKGFSISFEVTYTKLHKQLGWNIL 258
Query: 259 KTYKYIPKLLKELKDL--PISAWAVKMEYPVGNARSHANIFPSSTEFVKMTSEIWEWWEE 316
K YKY + P+ W ++ N R +I E + E +
Sbjct: 259 KIYKYFYTEFNAANVIISPVYDWNGSLD---KNLRIDISIAQEYYEIIASLLEKSRRGDL 315
Query: 317 SNLKRIEFVEDLGYFPPAIKKPAKKRAYYLCSAGVTQVTIDAEGNITPCTLIQITDGSTK 376
++ R + L + K C AG +D GNI PC Q+ G+ K
Sbjct: 316 NDFDRALLCKLLMNYLNPSDSSGKIIVSTFCPAGSNSFIVDIFGNIYPC---QLVVGNLK 372
Query: 377 YIAGNLYYDS 386
Y N+ D+
Sbjct: 373 YRISNVVKDN 382
>gb|AAK42036.1| Coenzyme PQQ synthesis protein E (pqqE-5) [Sulfolobus solfataricus]
Length = 350
Score = 77.6 bits (188), Expect = 3e-13
Identities = 82/360 (22%), Positives = 143/360 (38%), Gaps = 47/360 (13%)
Query: 110 PGAIAVEITHRCNLKCEHCYVGTRNNP--ATLSLEVIKKLIHDMEKLGCYQLAIGGGEPT 167
P + E+T C L C+HC + P LS E KKL+ D+ + G + GG+P
Sbjct: 7 PHLVFWEVTKACPLSCKHCRANAIDKPLPGELSTEESKKLLEDIARFGKVVIVFTGGDPL 66
Query: 168 LHPNFEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGP-KEIHEK 226
+ +I+E + I + + L + K + R ++ISIDG + H+
Sbjct: 67 SRSDIFEIMEYAKSLGLIVSIAPSPSYRLRDETMKMISNYARY--MSISIDGATSQTHDW 124
Query: 227 VRKSVKFEEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYP 286
+R + + I+ + G + Q+ T V K+Y +P L+K LK+L + W V P
Sbjct: 125 LRGLGSYNYALRGIELGLKYGIQVQVNTLVWKKSYSELPFLVKLLKELGVKVWEVFFLIP 184
Query: 287 VGNARSHANI----FPSSTEFVKMTSE---IWEWWEESNLKRIEFVEDLGYFPPAIK--- 336
VG +I + +F+ T+ + E +R + L Y P IK
Sbjct: 185 VGRGVIELDIPKEKYKDVIDFLVETTRYDLVVRTVEAPFFRRAK----LEYTPSTIKSNE 240
Query: 337 ----------KPAK--KRAYYLCSAGVTQVTIDAEGNITPCTLIQITDGSTKYIAGNLYY 384
+P K ++ G + I G++ P G GN+
Sbjct: 241 LVSKLRELLGEPVKDVDKSVLPTRDGSGVIFIGYNGDVYP-------SGFLPLYLGNVRK 293
Query: 385 DSLIEVWHTSNVLWEFRLMWPENESCARCGL-----VCAKCPATVLGIKGDIRKPDPRCP 439
+S+++++ S VL + + +CG+ +C A + + DP CP
Sbjct: 294 ESIVDIYRKSEVLKIIK----DGRFDGKCGVCKFNNICGGSRARAFAVYNNPFAEDPMCP 349
>gb|AAK41779.1| Heme biosynthesis related protein [Sulfolobus solfataricus]
Length = 394
Score = 74.5 bits (180), Expect = 2e-12
Identities = 83/348 (23%), Positives = 154/348 (43%), Gaps = 56/348 (16%)
Query: 79 ATTTEQEEFIEELMEYNVIMPYDPINPKFFPPGAIAVEITHRCNLKCEHCYVGTRNNPAT 138
AT + +E IE+ + V+ P + +T+ CN C +C+ +
Sbjct: 52 ATDEDLDEEIEKFLRKPVLEP--------------TLVLTYNCNFDCIYCFQKGFRKNIS 97
Query: 139 LSLEVIKKLIHDM---EKLGCYQLAIGGGEPTLH-PNFEKILEIIHKSKIFAHIVTNGTT 194
+S +V + ++ + EK ++ GGEP L E+I + K +VTNG+
Sbjct: 98 VSDKVTRGFVNYIRKNEKGRKVRVTYFGGEPILQLRKIEEISRELSDLKYSFSVVTNGSL 157
Query: 195 NLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRKSVK----FEEIINNIKKLAELGWKP 250
+ +E+ ++T+ DGPKE+H+K R V F+ I+ N+K++ ++ K
Sbjct: 158 LTRKIVERLIPLGLTHVQITL--DGPKEVHDKRRFFVSGEGTFDVIVKNLKEIQDM-VKV 214
Query: 251 QIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYPVGNARSHANIFPSSTEFVKMTSEI 310
++ + +K I +LL ELK I+ V+++ + H+N+F +
Sbjct: 215 VLRINIDVKNLNEIGELLNELKMEGIN--RVRLDPHL----VHSNVFRN----------- 257
Query: 311 WEWWEESNLKRIEFVEDLGYFPPAIK----KPAKKRAYYLCSAGVTQ-VTIDAEGNITPC 365
EWW+ + + E + ++ A K P LC A ++ + +D EGNI PC
Sbjct: 258 -EWWDFTISSKAEGDVMVKFWEIARKYGFEVPQDVLRLGLCVAYSSEDIVVDPEGNIYPC 316
Query: 366 TLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEFRLMWPENESCARC 413
G+ Y+ G L D ++V + S + W+ ++ + C C
Sbjct: 317 WAF---TGNPLYVKGKLEEDGSVKVTNKS-LWWKVKI----EDKCREC 356
>gb|AAK41844.1| Coenzyme PQQ synthesis protein E (pqqE-2) [Sulfolobus solfataricus]
Length = 350
Score = 73.0 bits (176), Expect = 7e-12
Identities = 74/356 (20%), Positives = 147/356 (40%), Gaps = 39/356 (10%)
Query: 110 PGAIAVEITHRCNLKCEHCYVGTRNNP--ATLSLEVIKKLIHDMEKLGCYQLAIGGGEPT 167
P + E+T C L C+HC + P L+ E +KL+ D+ + G + GG+P
Sbjct: 7 PHLVFWEVTKACPLTCKHCRANAIDKPLPGELNTEESRKLLEDIARFGKVVIVFTGGDPL 66
Query: 168 LHPNFEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGP-KEIHEK 226
+ +++E + I + + L + K + R ++IS+DG + H+
Sbjct: 67 SRSDIFELMEYAKSLGLVVSIAPSPSHRLDDETMKIISNYARY--MSISLDGATSQTHDW 124
Query: 227 VRKSVKFEEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEYP 286
+R ++ + I+ + G + Q+ T V K+Y +P ++K LK++ + W V P
Sbjct: 125 LRGLGSYKYALRGIELGLKYGIQVQVNTLVWKKSYSELPFVVKLLKEMGVKIWEVFFLIP 184
Query: 287 VGNARSHANI----FPSSTEFVKMTSE---IWEWWEESNLKR--IEFV-------EDLGY 330
VG + +I + +F+ + I E +R +E+ E +
Sbjct: 185 VGRGTTELDIPRDKYKDVIDFLVEATRYDLIVRTVEAPFFRRAKLEYTPATTNDNELVST 244
Query: 331 FPPAIKKPAKK--RAYYLCSAGVTQVTIDAEGNITPCTLIQITDGSTKYIAGNLYYDSLI 388
+ +P K+ ++ G + I G++ P G GN+ +SL+
Sbjct: 245 LRELLGEPVKEADKSILPTRDGAGVIFIGYNGDVYP-------SGFLPLYLGNVKKESLV 297
Query: 389 EVWHTSNVLWEFRLMWPENESCARCGL-----VCAKCPATVLGIKGDIRKPDPRCP 439
+++ S VL + + ++ +CG+ +C A + + DP CP
Sbjct: 298 DIYRKSEVLKKIK----DSRFEGKCGICKYNNICGGSRARAFAVYNNPFAEDPMCP 349
>emb|CAC12477.1| (AL445067) heme biosynthesis protein (NirJ) related protein
[Thermoplasma acidophilum]
Length = 341
Score = 71.4 bits (172), Expect = 2e-11
Identities = 81/317 (25%), Positives = 132/317 (41%), Gaps = 57/317 (17%)
Query: 116 EITHRCNLKCEHCYVGTRNNPATLSLEVIKKLIHDMEKLGCYQLAIGGGEPTLHPNFEKI 175
++ + CNL+C+ C R + LSLE K++ + G + GGEP L + E I
Sbjct: 36 KLLYTCNLRCDMCPFWRRKDEKLLSLEEEIKMMDSLASAGVSFMGFEGGEPLLRNDLEDI 95
Query: 176 LEIIHKSKIFAHIVTNG------TTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRK 229
L+ HK + +VTNG +++S YL+ + +SIDG KE H+K R
Sbjct: 96 LKESHK-RFHTSLVTNGWLLKGRASSISRYLDY----------LFVSIDGRKETHDKQRG 144
Query: 230 -SVKFEEIINNIK---KLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVKMEY 285
F+ I IK K + I TT ++ KY+ L +EL + ++ +++ Y
Sbjct: 145 VPSSFDHAIEGIKEAKKYVPVAMSTTI-TTNNMDDIKYVVNLAEEL-GIGVN---IQVSY 199
Query: 286 PVGNARSHANIFPSSTEFVKMTSEIWEWWEESNLKRIEFVEDLGYFPPAIKKPAKKRAYY 345
A + P + + ++ + E+ V YF + YY
Sbjct: 200 DYSTADK---LSPDRKKLYDLLLDLKKMKEDG----APIVNSKEYFDTLVNS-----WYY 247
Query: 346 ----LCSAGVTQVTIDAEGNIT-PCTLIQITDGSTKYIAGNLYYDSLIEVWHTSNVLWEF 400
C T V ID +G I PC ++ G K ++ + E+W+T +
Sbjct: 248 GIPWACKPWST-VNIDPQGRIVMPCYVLNEYAGEKK-----VWEIDIKELWNTYD----- 296
Query: 401 RLMWPENESCARCGLVC 417
W ESC +C L C
Sbjct: 297 ---WKPYESCNKCSLSC 310
>pir||C83581 heme d1 biosynthesis protein NirJ PA0511 [imported] - Pseudomonas
aeruginosa (strain PAO1) >gi|1783281|dbj|BAA12681.1|
(D84475) NirJ [Pseudomonas aeruginosa]
>gi|9946376|gb|AAG03900.1|AE004488_4 (AE004488) heme d1
biosynthesis protein NirJ [Pseudomonas aeruginosa]
Length = 387
Score = 70.6 bits (170), Expect = 3e-11
Identities = 49/182 (26%), Positives = 91/182 (49%), Gaps = 6/182 (3%)
Query: 117 ITHRCNLKCEHCYVGTRNNPATLSLEVIKKL--IHDMEKLGCYQLAIGGGEPTLHPNFEK 174
+ RCNL C+HCY + ++ LE + L I D+ G L + GGEP +HP+ +
Sbjct: 33 LLRRCNLTCKHCYSTSADSDFRGELETAEILRGIDDLRAAGVRVLILSGGEPLMHPDLFE 92
Query: 175 ILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVR-KSVKF 233
I ++ +F + +NGT +++ A + R V IS+DG +E H++ R K F
Sbjct: 93 IAAHARQAGMFVALSSNGTLIDEGNIQRVA--EARFDYVGISLDGLRETHDRFRQKQGSF 150
Query: 234 EEIINNIKKLAELGWKPQIQTTVSLKTYKYIPKLLKELKDLPISAWAVK-MEYPVGNARS 292
+ + ++ E + ++TT++ + +P LL +++L + + + + Y RS
Sbjct: 151 DAALAAMRLCREADIRVGMRTTLTEENAAQLPALLDLMRELDVQKFYLSHLNYSGRGRRS 210
Query: 293 HA 294
A
Sbjct: 211 RA 212
>pir||D75071 tungsten-containing aldehyde ferredoxin oxidoreductase cofactor
modifying protein PAB0648 - Pyrococcus abyssi (strain
Orsay) >gi|5458385|emb|CAB49873.1| (AJ248286)
tungsten-containing aldehyde ferredoxin oxidoreductase
COFACTOR MODIFYING PROTEIN [Pyrococcus abyssi]
Length = 373
Score = 69.9 bits (168), Expect = 6e-11
Identities = 78/324 (24%), Positives = 134/324 (41%), Gaps = 40/324 (12%)
Query: 115 VEITHRCNLKCEHCYVG-TRNNPATLSLEVIKKLIHDMEKLGCYQLAIGG--GEPTLHPN 171
+EIT+RCNLKCE C+ + + E+ K++ D E+L ++ G GEP++HP
Sbjct: 23 LEITNRCNLKCEMCFKQYWEDEEGDMDWELFLKVLGDAEELPELKMIYFGGIGEPSVHPR 82
Query: 172 FEKILEIIHKSKIFAHIVTNGTTNLSEYLEKYANKKKRSFEVTISIDGPKEIHEKVRKSV 231
F ++ + + + +NG + LE++ K + S+D ++
Sbjct: 83 FMDMVREVKERGFRLGMSSNGVLLNDKILEEFV--KLGVDVIYFSMDAIPTGENLIKLGH 140
Query: 232 KFEEII-NNIKKLAEL-----GWKPQI--QTTVSLKTYKYIPKLLKELKDLPISAWAVKM 283
+I+ N IKKL EL KP I + + + YK +P + L+ L + + +
Sbjct: 141 VTSKIVENRIKKLVELKEKYGSEKPTIGVEVVATKENYKELPDMALYLRKLGVDSMLISN 200
Query: 284 EYPVGNARSHANIFPSSTEFVKMTSEIWEWWEESNLKRI-EFVEDLGYFPPAIKKPAKKR 342
P+ SH ++ S + + +++ E RI EF K R
Sbjct: 201 LIPLSKEHSHLILYDGSVDMTPIVNKLHSIAEGGPFMRIAEF---------------KLR 245
Query: 343 AYYLCSAGVTQVTIDA-EGNITPC-----TLIQITDGSTKYIA----GNLYYDSLIEVWH 392
C VT+ +G + PC T ++ G K + GN+ SL E+W
Sbjct: 246 TERYCDFVENNVTVVRWDGEVFPCYRFLHTYPEVIFGREKKVIAHSFGNVREKSLKEIWM 305
Query: 393 TSN-VLWEFRLMWPENESCARCGL 415
+ V + F + SC C L
Sbjct: 306 SEEYVRFRFIVKASAYPSCIDCPL 329
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.138 0.430
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 180738869
Number of Sequences: 2977
Number of extensions: 7915590
Number of successful extensions: 18535
Number of sequences better than 1.0e-10: 16
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 18500
Number of HSP's gapped (non-prelim): 20
length of query: 441
length of database: 189,106,746
effective HSP length: 53
effective length of query: 388
effective length of database: 157,386,935
effective search space: 61066130780
effective search space used: 61066130780
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 166 (69.1 bits)