BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1720 (pncA) DE:PYRAZINAMIDASE/NICOTINAMIDASE
(180 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||F75073 pyrazinamidase/nicotinamidase PAB1720 - Pyrococcus a... 373 e-103
pir||B71092 probable pyrazinamidase/nicotinamidase - Pyrococcus ... 344 4e-94
pir||B70386 pyrazinamidase/nicotinamidase - Aquifex aeolicus >gi... 224 4e-58
pir||E82427 pyrazinamidase/nicotinamidase VCA0712 [imported] - V... 147 9e-35
sp|P21369|PNCA_ECOLI PYRAZINAMIDASE/NICOTINAMIDASE [INCLUDES: PY... 136 2e-31
pir||QQECA5 hypothetical 23.4K protein (ansA 3' region) - Escher... 136 2e-31
emb|CAB60666.1| (Y09633) hypothetical protein [Bradyrhizobium ja... 133 1e-30
gi|11499734 isochorismatase (entB) [Archaeoglobus fulgidus] >gi|... 130 1e-29
gi|11466135 PXNC; L4171.2 [Leishmania major] >gi|7444686|pir||T0... 127 1e-28
gb|AAF76877.1|AF247710_2 (AF247710) putative pyrazinamidase/nico... 121 6e-27
gi|6321401 pyrazinamidase and nicotinamidase; Pnc1p [Saccharomyc... 113 1e-24
pir||B70944 probable pncA protein - Mycobacterium tuberculosis (... 109 2e-23
gi|11496610 pyrazinamidase/nicotinamidase (pncA) [Borrelia burgd... 108 5e-23
pir||C72372 pyrazinamidase/nicotinamidase-related protein - Ther... 108 5e-23
pir||T36141 probable nicotinamidase - Streptomyces coelicolor >g... 108 6e-23
dbj|BAA88679.1| (AB029373) ORF [Thermus thermophilus] 106 2e-22
gb|AAD11442.1| (AF117900) nicotinamidase/pyrazinamidase [Mycobac... 100 1e-20
gb|AAB84064.1| (U80820) pyrazinamidase/nicotinamidase [Mycobacte... 98 7e-20
pir||T50371 probable pyrazinamidase/nicotinamidase [imported] - ... 98 7e-20
gb|AAC78716.1| (AF002663) nicotinamidase [Mycobacterium kansasii] 98 9e-20
gb|AAF55694.1| (AE003727) CG7500 gene product [Drosophila melano... 92 4e-18
gb|AAK42595.1| Isochorismatase, putative (entB-like2) [Sulfolob... 90 1e-17
emb|CAB39030.1| (AL034559) hypothetical protein, PFC0910w [Plasm... 85 7e-16
emb|CAC11596.1| (AL445064) N-carbamoylsarcosine amidase related ... 83 1e-15
pir||D83030 hypothetical protein PA4918 [imported] - Pseudomonas... 81 7e-15
pir||C72463 hypothetical protein APE2350 - Aeropyrum pernix (str... 76 3e-13
>pir||F75073 pyrazinamidase/nicotinamidase PAB1720 - Pyrococcus abyssi (strain
Orsay) >gi|5458403|emb|CAB49891.1| (AJ248286)
PYRAZINAMIDASE/NICOTINAMIDASE [Pyrococcus abyssi]
Length = 180
Score = 373 bits (948), Expect = e-103
Identities = 180/180 (100%), Positives = 180/180 (100%)
Query: 1 MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHIS 60
MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHIS
Sbjct: 1 MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHIS 60
Query: 61 FKERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEGTNLDKILKEKGV 120
FKERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEGTNLDKILKEKGV
Sbjct: 61 FKERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEGTNLDKILKEKGV 120
Query: 121 KRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIKIVTL 180
KRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIKIVTL
Sbjct: 121 KRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIKIVTL 180
>pir||B71092 probable pyrazinamidase/nicotinamidase - Pyrococcus horikoshii
>gi|3257413|dbj|BAA30096.1| (AP000004) 180aa long
hypothetical pyrazinamidase/nicotinamidase [Pyrococcus
horikoshii]
Length = 180
Score = 344 bits (873), Expect = 4e-94
Identities = 161/178 (90%), Positives = 173/178 (96%)
Query: 1 MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHIS 60
MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYI+KF+EKGALIVATRDWHPENHIS
Sbjct: 1 MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIRKFKEKGALIVATRDWHPENHIS 60
Query: 61 FKERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEGTNLDKILKEKGV 120
F+ERGGPWP+HCVQNTPGAEFVVDLPED +IISKATEPDKEAYSGFEGT+L KIL+ GV
Sbjct: 61 FRERGGPWPRHCVQNTPGAEFVVDLPEDAVIISKATEPDKEAYSGFEGTDLAKILRGNGV 120
Query: 121 KRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIKIV 178
KRVY+CGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEE+AL+EM+ +GIKIV
Sbjct: 121 KRVYICGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEERALEEMKSRGIKIV 178
>pir||B70386 pyrazinamidase/nicotinamidase - Aquifex aeolicus
>gi|2983496|gb|AAC07074.1| (AE000717)
pyrazinamidase/nicotinamidase [Aquifex aeolicus]
Length = 198
Score = 224 bits (566), Expect = 4e-58
Identities = 103/182 (56%), Positives = 137/182 (74%), Gaps = 5/182 (2%)
Query: 4 EALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFKE 63
+ALIVVDMQ+DFMPGGALPVPEGDKIIP++NEY+K F +KG + TRDWHPENHISFK
Sbjct: 9 DALIVVDMQKDFMPGGALPVPEGDKIIPRINEYVKLFEKKGLPVFYTRDWHPENHISFKG 68
Query: 64 RGGPWPKHCVQNTPGAEFVVDL---PEDTIIISKATEPDKEAYSGFEGTNLDKILKEKGV 120
GG WP HCVQ T GA+F DL ++ IISK T + +AYSGF+GT L+ +LKE+GV
Sbjct: 69 HGGIWPPHCVQGTEGAKFHEDLYIPADNKFIISKGTSQEFDAYSGFQGTILNDLLKERGV 128
Query: 121 KRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKG--IKPEDEEKALKEMEEKGIKIV 178
+R++VCGVAT+YCV+ T + + G++ ++L DA+KG + P D E+A+ EM E+G +
Sbjct: 129 RRIFVCGVATDYCVKNTTIGGINLGYQAFVLEDAIKGVDVNPGDVERAINEMMERGAAFI 188
Query: 179 TL 180
+
Sbjct: 189 KI 190
>pir||E82427 pyrazinamidase/nicotinamidase VCA0712 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9658131|gb|AAF96611.1|
(AE004400) pyrazinamidase/nicotinamidase [Vibrio
cholerae]
Length = 206
Score = 147 bits (367), Expect = 9e-35
Identities = 77/201 (38%), Positives = 117/201 (57%), Gaps = 29/201 (14%)
Query: 4 EALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFKE 63
+AL++VD+Q DF P GALPVP+GD ++P +N+ + F ++AT+DWHP H SF
Sbjct: 3 KALLLVDVQNDFSPSGALPVPDGDAVVPVINQLLPLFEH----VIATKDWHPAKHASFAS 58
Query: 64 RGGP---------------WPKHCVQNTPGAEFV--VDLPEDTIIISKATEPDKEAYSGF 106
G WP HCVQNT GAEF+ +D+ T I++K T D ++YSGF
Sbjct: 59 VQGKTPGEVIDLNGIPQVMWPDHCVQNTHGAEFIAGLDVQRITHIVTKGTHLDVDSYSGF 118
Query: 107 ------EGTNLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIK- 159
T L + L +G+ VY+ G+AT+YCV+ TALDA+ F+ ++++DA +G++
Sbjct: 119 FDNQRFHATGLAEYLSNQGISEVYIAGLATDYCVKYTALDAVSLYFKTWVIQDACRGVEL 178
Query: 160 -PEDEEKALKEMEEKGIKIVT 179
D A M+ G +++T
Sbjct: 179 NAGDCATAWHAMQTAGCQLIT 199
>sp|P21369|PNCA_ECOLI PYRAZINAMIDASE/NICOTINAMIDASE [INCLUDES: PYRAZINAMIDASE (PZASE);
NICOTINAMIDASE (NICOTINE DEAMIDASE)]
>gi|145280|gb|AAA23447.1| (M26934) ORF1 [Escherichia
coli] >gi|1742879|dbj|BAA15559.1| (D90820)
ORF_ID:o329#5; similar to [PIR Accession Number JU0048]
[Escherichia coli]
Length = 213
Score = 136 bits (338), Expect = 2e-31
Identities = 76/205 (37%), Positives = 111/205 (54%), Gaps = 25/205 (12%)
Query: 1 MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHIS 60
MP AL++VD+Q DF GGAL VPEGD + N I + +G ++A++DWHP NH S
Sbjct: 1 MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGS 60
Query: 61 FKERGGP---------------WPKHCVQNTPGAEFVVDLPEDTI--IISKATEPDKEAY 103
F + G WP HCVQN+ GA+ L + I + K P ++Y
Sbjct: 61 FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSY 120
Query: 104 SGF------EGTNLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKG 157
S F + T+LD L++ + + V G+AT+YCV+ T LDAL+ G++V ++ D +G
Sbjct: 121 SAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTVLDALQLGYKVNVITDGCRG 180
Query: 158 --IKPEDEEKALKEMEEKGIKIVTL 180
I+P+D A EM G + TL
Sbjct: 181 VNIQPQDSAHAFMEMSAAGATLYTL 205
>pir||QQECA5 hypothetical 23.4K protein (ansA 3' region) - Escherichia coli
>gi|1788066|gb|AAC74838.1| (AE000271) orf, hypothetical
protein [Escherichia coli K12]
Length = 219
Score = 136 bits (338), Expect = 2e-31
Identities = 76/205 (37%), Positives = 111/205 (54%), Gaps = 25/205 (12%)
Query: 1 MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHIS 60
MP AL++VD+Q DF GGAL VPEGD + N I + +G ++A++DWHP NH S
Sbjct: 7 MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGS 66
Query: 61 FKERGGP---------------WPKHCVQNTPGAEFVVDLPEDTI--IISKATEPDKEAY 103
F + G WP HCVQN+ GA+ L + I + K P ++Y
Sbjct: 67 FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQKAIAAVFHKGENPLVDSY 126
Query: 104 SGF------EGTNLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKG 157
S F + T+LD L++ + + V G+AT+YCV+ T LDAL+ G++V ++ D +G
Sbjct: 127 SAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFTVLDALQLGYKVNVITDGCRG 186
Query: 158 --IKPEDEEKALKEMEEKGIKIVTL 180
I+P+D A EM G + TL
Sbjct: 187 VNIQPQDSAHAFMEMSAAGATLYTL 211
>emb|CAB60666.1| (Y09633) hypothetical protein [Bradyrhizobium japonicum]
Length = 240
Score = 133 bits (331), Expect = 1e-30
Identities = 77/197 (39%), Positives = 108/197 (54%), Gaps = 29/197 (14%)
Query: 5 ALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFKE- 63
AL+V+D+Q F+PGG+L V EG++++P +N+ K F +V T+DWH H+SF
Sbjct: 37 ALLVIDVQNCFLPGGSLAVKEGEQVVPVINKISKAFSN----VVMTQDWHTPGHVSFASV 92
Query: 64 ---------------RGGPWPKHCVQNTPGAEFVVDL--PEDTIIISKATEPDKEAYSGF 106
+ WP HCVQ T GA DL P +II K D ++YS F
Sbjct: 93 HSGKKPFETIDLPYGKQVLWPDHCVQGTDGASLSKDLAIPHAELIIRKGFHKDVDSYSAF 152
Query: 107 ------EGTNLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKP 160
T L LK + +KRV+V G+AT++CV TALDA K GFEVY++ DA +GI
Sbjct: 153 LEADGKTSTGLAGYLKGRKIKRVFVAGLATDFCVAWTALDARKAGFEVYVVEDACRGIDT 212
Query: 161 EDE-EKALKEMEEKGIK 176
+ KA +M + G+K
Sbjct: 213 QGSLAKAWADMAKAGVK 229
>gi|11499734 isochorismatase (entB) [Archaeoglobus fulgidus]
>gi|7429496|pir||G69518 isochorismatase (entB) homolog -
Archaeoglobus fulgidus >gi|2648379|gb|AAB89104.1|
(AE000956) isochorismatase (entB) [Archaeoglobus
fulgidus]
Length = 175
Score = 130 bits (323), Expect = 1e-29
Identities = 73/172 (42%), Positives = 103/172 (59%), Gaps = 16/172 (9%)
Query: 4 EALIVVDMQRDFM-PGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFK 62
EAL+VVDMQ+DF GAL +P ++I + ++ R++ +I T+DWH E+ + FK
Sbjct: 2 EALVVVDMQKDFCYKSGALYIPNAEEIFEATAKVVEAARKRMPVIF-TQDWHREDDVEFK 60
Query: 63 ERGGPWPKHCVQNTPGAEFVVDL---PEDTIIISKATEPDKEAYSGFEGTNLDKILKEKG 119
WPKHCV NT GAE + +L PED + K YS F T+LD +L+E G
Sbjct: 61 I----WPKHCVMNTEGAEVIDELNPQPEDYYV-------KKRRYSAFFATDLDLLLRELG 109
Query: 120 VKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEME 171
VK++Y+CGVAT CV TA DA+ G+EV +L+D K + D E L+ M+
Sbjct: 110 VKKLYICGVATNICVLHTAGDAVLRGYEVAVLKDCTKALSDYDYEYGLRHMK 161
>gi|11466135 PXNC; L4171.2 [Leishmania major] >gi|7444686|pir||T02830
pyrazinamidase/nicotinamidase PXNC [imported] -
Leishmania major (strain Friedlin)
>gi|2266908|gb|AAC24653.1| (AE001274) PXNC; L4171.2
[Leishmania major]
Length = 218
Score = 127 bits (315), Expect = 1e-28
Identities = 72/186 (38%), Positives = 103/186 (54%), Gaps = 14/186 (7%)
Query: 4 EALIVVDMQRDFM-PGGALPVPEGDKIIPKVNEYIKK--FREKGALIVATRDWHPENHIS 60
+ LI+ DMQ DF+ PGG+L V G+ ++ +N + FR + VAT+DWHPENH S
Sbjct: 26 DVLIIADMQVDFLTPGGSLHVKGGEALLDGINAVSSQLPFRYQ----VATQDWHPENHCS 81
Query: 61 FKERGGPWPKHCVQNTPGAEFVVDLPEDTI--IISKATEPDKEAYSGF-----EGTNLDK 113
F GGPWP HCVQ + GA+ L I ++ K ++YS F T L
Sbjct: 82 FVTHGGPWPPHCVQGSSGAQLHAGLHTHRINAVLRKGATQQADSYSAFVDDNGVSTGLAG 141
Query: 114 ILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEK 173
+L G +RV+VCGVA ++CV TA+DA K+GF V LL D + E++E
Sbjct: 142 LLHSIGARRVFVCGVAYDFCVFFTAMDARKNGFGVVLLEDLTAAVDDAAWSARAAELKEA 201
Query: 174 GIKIVT 179
G+ +++
Sbjct: 202 GVVLLS 207
>gb|AAF76877.1|AF247710_2 (AF247710) putative pyrazinamidase/nicotinamidase PncA
[Sinorhizobium meliloti]
Length = 211
Score = 121 bits (300), Expect = 6e-27
Identities = 72/203 (35%), Positives = 106/203 (51%), Gaps = 29/203 (14%)
Query: 1 MPEEALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHIS 60
M +EALIV+DMQ DF PGGAL V GD+I+P VN I + +V T+DWHP H S
Sbjct: 13 MADEALIVIDMQNDFCPGGALAVEGGDEIVPAVNRLI----DASPHVVLTQDWHPAGHSS 68
Query: 61 FKE----------------RGGPWPKHCVQNTPGAEFVVDL--PEDTIIISKATEPDKEA 102
F WP+HCVQ + GA+F L ++I K + ++
Sbjct: 69 FASTHPGKAPFQTVAMPYGEQTLWPEHCVQGSAGADFHPSLRWTSAELVIRKGFRREIDS 128
Query: 103 YSGF------EGTNLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVK 156
YS F T L L+E+G++ V +CG+AT++CV +ALDA+ GF ++ A +
Sbjct: 129 YSAFFENDHRTPTGLAGYLRERGIRSVTLCGLATDFCVAFSALDAVAKGFSTSVVLGACR 188
Query: 157 GIKPEDEEKALK-EMEEKGIKIV 178
GI A+ M + G++++
Sbjct: 189 GIDLNGSLAAMTLRMRDAGVRLI 211
>gi|6321401 pyrazinamidase and nicotinamidase; Pnc1p [Saccharomyces cerevisiae]
>gi|1723821|sp|P53184|YGD7_YEAST HYPOTHETICAL 25.0 KD
PROTEIN IN OCH1-MTC2 INTERGENIC REGION
>gi|2131568|pir||S64039 hypothetical protein YGL037c -
yeast (Saccharomyces cerevisiae)
>gi|1322518|emb|CAA96739.1| (Z72559) ORF YGL037c
[Saccharomyces cerevisiae]
Length = 216
Score = 113 bits (280), Expect = 1e-24
Identities = 71/213 (33%), Positives = 109/213 (50%), Gaps = 38/213 (17%)
Query: 4 EALIVVDMQRDFM-PGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFK 62
+ LIVVDMQ DF+ P G+L VP+G+++I +++ ++ IV TRDWHP HISF
Sbjct: 2 KTLIVVDMQNDFISPLGSLTVPKGEELINPISDLMQDADRDWHRIVVTRDWHPSRHISFA 61
Query: 63 ------------------------ERGGPWPKHCVQNTPGAEFVVDLPEDTI-----IIS 93
+ G WP HCV+NT G++ V + + + I+
Sbjct: 62 KNHKDKEPYSTYTYHSPRPGDDSTQEGILWPVHCVKNTWGSQLVDQIMDQVVTKHIKIVD 121
Query: 94 KATEPDKEAYSGFEG------TNLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFE 147
K D+E YS F T+++K L++ VY+ GVA EYCV+ATA+ A + G++
Sbjct: 122 KGFLTDREYYSAFHDIWNFHKTDMNKYLEKHHTDEVYIVGVALEYCVKATAISAAELGYK 181
Query: 148 VYLLRDAVKGIK--PEDEEKALKEMEEKGIKIV 178
+L D + I PE K +E++ I +V
Sbjct: 182 TTVLLDYTRPISDDPEVINKVKEELKAHNINVV 214
>pir||B70944 probable pncA protein - Mycobacterium tuberculosis (strain H37RV)
>gi|1399892|gb|AAB37768.1| (U59967)
pyrazinamidase/nicotinamidase [Mycobacterium
tuberculosis] >gi|2896780|emb|CAA17257.1| (AL021899)
pncA [Mycobacterium tuberculosis]
Length = 186
Score = 109 bits (270), Expect = 2e-23
Identities = 67/182 (36%), Positives = 99/182 (53%), Gaps = 9/182 (4%)
Query: 5 ALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWH--PENHIS-F 61
ALI+VD+Q DF GG+L V G + +++Y+ + + +VAT+D+H P +H S
Sbjct: 3 ALIIVDVQNDFCEGGSLAVTGGAALARAISDYLAEAADYHH-VVATKDFHIDPGDHFSGT 61
Query: 62 KERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEG-----TNLDKILK 116
+ WP HCV TPGA+F L I AYSGFEG T L L+
Sbjct: 62 PDYSSSWPPHCVSGTPGADFHPSLDTSAIEAVFYKGAYTGAYSGFEGVDENGTPLLNWLR 121
Query: 117 EKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIK 176
++GV V V G+AT++CVR TA DA+++G +L D G+ + AL+EM ++
Sbjct: 122 QRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLTAGVSADTTVAALEEMRTASVE 181
Query: 177 IV 178
+V
Sbjct: 182 LV 183
>gi|11496610 pyrazinamidase/nicotinamidase (pncA) [Borrelia burgdorferi]
>gi|7445160|pir||A70227 pyrazinamidase/nicotinamidase
(pncA) homolog - Lyme disease spirochete plasmid E/lp25
>gi|2689954|gb|AAC66025.1| (AE000785)
pyrazinamidase/nicotinamidase (pncA) [Borrelia
burgdorferi]
Length = 178
Score = 108 bits (267), Expect = 5e-23
Identities = 58/154 (37%), Positives = 88/154 (56%), Gaps = 15/154 (9%)
Query: 21 LPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFK--ERGGPWPKHCVQNTPG 78
+PV ++II +N+ F+ I+AT+DWH +NH+SF + GG WP+HCV+NT G
Sbjct: 1 MPVSNSNEIISLINQLQNYFKN----IIATKDWHCKNHVSFSNNKNGGIWPEHCVKNTWG 56
Query: 79 AEFVVDLPEDTI--IISKATEPDKEAYSGF-------EGTNLDKILKEKGVKRVYVCGVA 129
+EF DL I + K T+ ++YSGF + T L LK + +++ G+A
Sbjct: 57 SEFPNDLNTKRIKKVFFKGTDQYYDSYSGFYDDCIKKKQTGLQLYLKNNSINTLFITGLA 116
Query: 130 TEYCVRATALDALKHGFEVYLLRDAVKGIKPEDE 163
++CV+ T LDA+ GF VYL+ DA + I E
Sbjct: 117 LDFCVKETILDAINLGFRVYLITDATRSITSTPE 150
>pir||C72372 pyrazinamidase/nicotinamidase-related protein - Thermotoga maritima
(strain MSB8) >gi|4980985|gb|AAD35560.1|AE001725_5
(AE001725) pyrazinamidase/nicotinamidase-related protein
[Thermotoga maritima]
Length = 214
Score = 108 bits (267), Expect = 5e-23
Identities = 59/169 (34%), Positives = 88/169 (51%), Gaps = 5/169 (2%)
Query: 5 ALIVVDMQRDFMP-GGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFKE 63
AL+VVD+QRDF+ GGAL +K+I + +++++F+++ I+ T+DWH F
Sbjct: 43 ALLVVDLQRDFVDEGGALYFEGAEKVINPILKWVEEFKKENLPIITTQDWHDPEDREFNI 102
Query: 64 RGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEGTNLDKILKEKGVKRV 123
WPKHCV NT GA L + K YS F TNL+KI+++ + +
Sbjct: 103 ----WPKHCVANTDGARLTEKLEKALKDYPNHFSVKKNRYSAFYNTNLEKIIRDNEIDEI 158
Query: 124 YVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEE 172
YVCGV T CV T + V ++ + V E AL+EM+E
Sbjct: 159 YVCGVVTHICVLFTVEELRNRDIPVKIITEGVASYDEELHRFALREMKE 207
>pir||T36141 probable nicotinamidase - Streptomyces coelicolor
>gi|5531367|emb|CAB50999.1| (AL096852) putative
nicotinamidase [Streptomyces coelicolor A3(2)]
Length = 195
Score = 108 bits (266), Expect = 6e-23
Identities = 70/185 (37%), Positives = 96/185 (51%), Gaps = 12/185 (6%)
Query: 5 ALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWH--PENHISFK 62
ALIVVD+Q DF GG+L V G + + E I + +VATRD H P H +
Sbjct: 4 ALIVVDVQNDFCEGGSLAVSGGADVAAAITELIGQAPAGYRHVVATRDHHVAPGGHFADN 63
Query: 63 -ERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDK----EAYSGFEG-----TNLD 112
+ WP HCV T G F + + DK AYSGFEG T L
Sbjct: 64 PDYVHSWPAHCVAGTEGVGFHPNFAPAVASGAIDAVFDKGAYAAAYSGFEGADENGTGLA 123
Query: 113 KILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEE 172
+ L+ + + V V G+AT++CVRATALDA + GF +L D G+ E E+AL+E+ E
Sbjct: 124 EWLRSREIDEVDVVGIATDHCVRATALDAAREGFRTQVLLDLTAGVAAETTERALEELRE 183
Query: 173 KGIKI 177
G+++
Sbjct: 184 AGVEL 188
>dbj|BAA88679.1| (AB029373) ORF [Thermus thermophilus]
Length = 196
Score = 106 bits (261), Expect = 2e-22
Identities = 68/172 (39%), Positives = 89/172 (51%), Gaps = 15/172 (8%)
Query: 3 EEALIVVDMQRDFM-PGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISF 61
E ALIVVDMQ DF P GAL VP+ + +P + +++ R+ GA +V T+DWH E+ F
Sbjct: 19 ETALIVVDMQNDFAHPKGALFVPDAPQSVPAIRLLLERARQAGAKVVYTQDWHREDDPEF 78
Query: 62 KERGGPWPKHCVQNTPGAEFVVDL---PEDTIIISKATEPDKEAYSGFEGTNLDKILKEK 118
+ WP+H V T GAE + +L PED +I K Y F GT LD L
Sbjct: 79 QI----WPRHAVAGTWGAEILEELKPEPEDLVI-------RKVRYDAFYGTPLDHYLHLF 127
Query: 119 GVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEM 170
GVK V V G CV TA A + V L DA + P D E L+++
Sbjct: 128 GVKHVVVTGTVANICVLHTAGSAALRWYNVVLPEDATSALTPFDLEATLRQV 179
>gb|AAD11442.1| (AF117900) nicotinamidase/pyrazinamidase [Mycobacterium smegmatis]
Length = 187
Score = 100 bits (247), Expect = 1e-20
Identities = 66/174 (37%), Positives = 89/174 (50%), Gaps = 9/174 (5%)
Query: 5 ALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWH--PENHIS-F 61
ALIVVD+Q DF GG+L V G + + E + +VAT D+H P H S
Sbjct: 3 ALIVVDVQNDFCEGGSLAVTGGAAVARGITELLAGDHGYDH-VVATMDFHIDPGEHFSDT 61
Query: 62 KERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEGTN-----LDKILK 116
+ WP+HCV +TPGAEF + + + AYSGFEGT+ L L+
Sbjct: 62 PDYQVSWPRHCVADTPGAEFHPEFDAGAVEVVFRKGHYSAAYSGFEGTDESGTTLADWLR 121
Query: 117 EKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEM 170
E+ V V V G+AT+YCV ATA DA GF +L G+ PE A+ ++
Sbjct: 122 ERDVDAVDVVGIATDYCVAATAADAAGAGFATRVLTRLTAGVAPESTTAAVDQL 175
>gb|AAB84064.1| (U80820) pyrazinamidase/nicotinamidase [Mycobacterium avium]
Length = 187
Score = 97.9 bits (240), Expect = 7e-20
Identities = 62/182 (34%), Positives = 91/182 (49%), Gaps = 8/182 (4%)
Query: 5 ALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWH--PENHISFK 62
ALI+VD+Q DF GG++PV G + P ++VAT+D+H P +H S +
Sbjct: 3 ALIIVDVQNDFCEGGSVPVAGGAAVAPPSTPTWTTLPGYDYVVVATQDFHIDPGDHFSDR 62
Query: 63 -ERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEG-----TNLDKILK 116
+ WP HC+ + GA+F +L + YSGFEG T L + L+
Sbjct: 63 PDYSSSWPAHCLAGSAGADFRPELDTTRVDAVFRKGAYAAGYSGFEGVDDNGTPLLEWLR 122
Query: 117 EKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIK 176
+GV V V G+AT++CVR TA DA + G +L + +AL EM GI+
Sbjct: 123 RRGVDEVDVVGIATDHCVRRTAEDAARAGLTTRVLVHLTAAAAEDSAARALDEMRSAGIE 182
Query: 177 IV 178
+V
Sbjct: 183 LV 184
>pir||T50371 probable pyrazinamidase/nicotinamidase [imported] - fission yeast
(Schizosaccharomyces pombe) (fragment)
>gi|6434007|emb|CAB60673.1| (AL132869) putative
pyrazinamidase/nicotinamidase [Schizosaccharomyces
pombe]
Length = 198
Score = 97.9 bits (240), Expect = 7e-20
Identities = 58/181 (32%), Positives = 94/181 (51%), Gaps = 31/181 (17%)
Query: 28 KIIPKVNEYIKKFREKGALIVATRDWHPENHISFKERGGPWPK----------------- 70
+++P +N ++ K ++AT+D HP++H+SF PK
Sbjct: 8 EVVPVINRLLEN-DYKWDTVIATKDVHPKDHLSFTTSHSSTPKPSGTVVNIEAYGHVYKQ 66
Query: 71 -----HCVQNTPGAEFVVDLPEDTI--IISKATEPDKEAYSGF-----EGTNLDKILKEK 118
HCV+NTPG EF L D I +I K ++ E+YSGF L IL +K
Sbjct: 67 TLWNSHCVENTPGCEFPDSLNGDRIEFVIPKGSDRLVESYSGFYDAIGRDNGLKAILDKK 126
Query: 119 GVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIKIV 178
G+ V++ GVAT+ CV+ TAL A +H + Y++ +AVKG E +A+K+ + I+++
Sbjct: 127 GITDVFIAGVATDICVKETALHA-RHWYNTYIISEAVKGSSTESHNQAIKDFRDAKIEVI 185
Query: 179 T 179
+
Sbjct: 186 S 186
>gb|AAC78716.1| (AF002663) nicotinamidase [Mycobacterium kansasii]
Length = 186
Score = 97.5 bits (239), Expect = 9e-20
Identities = 62/181 (34%), Positives = 94/181 (51%), Gaps = 9/181 (4%)
Query: 6 LIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWH--PENHISFK- 62
LI+VD+Q DF GG++PV D++ +N+Y+ R +VAT D+H P +H S +
Sbjct: 4 LIIVDLQNDFCEGGSVPVAGADRLARAINDYLAG-RPGYRHVVATEDFHIDPGDHFSDRP 62
Query: 63 ERGGPWPKHCVQNTPGAEFVVDLPEDTIIISKATEPDKEAYSGFEG-----TNLDKILKE 117
+ WP HC + GA+ DL + AYSG EG T L L++
Sbjct: 63 DYCSSWPAHCRAGSRGADIHPDLDTGQLEAVFRKGAYGAAYSGAEGVDEHGTTLVDWLRQ 122
Query: 118 KGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEMEEKGIKI 177
+G+ V V GVAT++CVR TA D + GF +L D G + ++AL ++ GI +
Sbjct: 123 RGIDEVDVVGVATDHCVRCTAEDMDRAGFATRVLVDLTAGASADTTDEALTQLRTAGIGL 182
Query: 178 V 178
+
Sbjct: 183 I 183
>gb|AAF55694.1| (AE003727) CG7500 gene product [Drosophila melanogaster]
Length = 328
Score = 92.1 bits (225), Expect = 4e-18
Identities = 63/208 (30%), Positives = 94/208 (44%), Gaps = 47/208 (22%)
Query: 2 PEEALIVVDMQRDFMPGG-----ALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPE 56
P A ++VD+Q DF+ G +G +I+ +N+ + + + DWHP
Sbjct: 66 PVNAFLIVDVQNDFISGSLDISNCSAQQQGHEILEPINKLLDTVDFDA--VFYSLDWHPS 123
Query: 57 NHISFKER-------------------------GGP-------WPKHCVQNTPGAEFVVD 84
+H+SF + GP WP+HCVQ++ GAE D
Sbjct: 124 DHVSFIDNVKMRPMDESSALDSDSAKVFDTVIFAGPPPMKQRLWPRHCVQDSWGAELHKD 183
Query: 85 LP--EDTIIISKATEPDKEAYSGF------EGTNLDKILKEKGVKRVYVCGVATEYCVRA 136
L + I + K T P+ ++YS F T L+ LK KG +YVCG+A + CV A
Sbjct: 184 LKVVDHGIKVYKGTNPEVDSYSVFWDNKKLSDTTLNAQLKMKGATDIYVCGLAYDVCVGA 243
Query: 137 TALDALKHGFEVYLLRDAVKGIKPEDEE 164
TA+DAL G+ L+ D +G D E
Sbjct: 244 TAVDALSAGYRTILIDDCCRGTDVHDIE 271
>gb|AAK42595.1| Isochorismatase, putative (entB-like2) [Sulfolobus solfataricus]
Length = 205
Score = 90.5 bits (221), Expect = 1e-17
Identities = 56/169 (33%), Positives = 85/169 (50%), Gaps = 11/169 (6%)
Query: 5 ALIVVDMQRDFM-PGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISFKE 63
ALI+VDMQ DF+ G L VP + IP + + K R AL++ T+DWH ++ FK
Sbjct: 29 ALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHMKDDPEFKI 88
Query: 64 RGGPWPKHCVQNTPGAEFVVDL-PEDTIIISKATEPDKEAYSGFEGTNLDKILKEKGVKR 122
W +H + T GAE + +L PE + I K K Y F ++LD IL+ K +K
Sbjct: 89 ----WGEHALAGTWGAEIIDELTPEKSDFIVK-----KYRYDAFFESSLDYILRVKNIKN 139
Query: 123 VYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEME 171
+ G CV TA A + V + +D++ I D L++++
Sbjct: 140 TIITGTVANICVLHTAGSAALRWYNVIMPKDSISAITEFDYYATLRQVD 188
>emb|CAB39030.1| (AL034559) hypothetical protein, PFC0910w [Plasmodium falciparum]
Length = 429
Score = 84.7 bits (206), Expect = 7e-16
Identities = 49/133 (36%), Positives = 75/133 (55%), Gaps = 10/133 (7%)
Query: 52 DWHPENHISFKERGGPWPKHCVQNTPGAEFVVDLPE--DTIIISKATEPDKEAYSGFEGT 109
++H N ++ E WP HCV+NT G + L + III KA + +K++++ FE
Sbjct: 294 EYHKINFLN--ENIDVWPVHCVKNTYGCKVHNKLIRHINDIIIKKAQKENKDSHTIFENE 351
Query: 110 ----NLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEK 165
N+ K+LK+K + VYVCG EYCV+ TAL L G+E Y++ DA + E+K
Sbjct: 352 QVNGNIQKLLKQKNITSVYVCGFIFEYCVKETALSFLNLGYETYIVEDATAYLFDRQEDK 411
Query: 166 ALKEMEEKGIKIV 178
++ KGIK +
Sbjct: 412 LF--LQNKGIKFI 422
>emb|CAC11596.1| (AL445064) N-carbamoylsarcosine amidase related protein
[Thermoplasma acidophilum]
Length = 182
Score = 83.5 bits (203), Expect = 1e-15
Identities = 54/158 (34%), Positives = 80/158 (50%), Gaps = 23/158 (14%)
Query: 5 ALIVVDMQRDFMPGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWH----PENHIS 60
AL+VVDM +F+ G L PE K + + I+ FR G +V D H PE I
Sbjct: 4 ALVVVDMVNEFIHG-RLATPEAMKTVGPARKVIETFRRSGLPVVYVNDSHYPDDPEIRI- 61
Query: 61 FKERGGPWPKHCVQNTPGAEFVVDLPE---DTIIISKATEPDKEAYSGFEGTNLDKILKE 117
W +H ++ G+E + ++ D ++ +K AYSGF GTNLD IL+
Sbjct: 62 -------WGRHSMKGDDGSEVIDEIRPSAGDYVL-------EKHAYSGFYGTNLDMILRA 107
Query: 118 KGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAV 155
G+ V + G+ + CVR TA DAL + + ++ DAV
Sbjct: 108 NGIDTVVLIGLDADICVRHTAADALYRNYRIIVVEDAV 145
>pir||D83030 hypothetical protein PA4918 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9951196|gb|AAG08303.1|AE004905_1
(AE004905) hypothetical protein [Pseudomonas aeruginosa]
Length = 219
Score = 81.1 bits (197), Expect = 7e-15
Identities = 57/192 (29%), Positives = 85/192 (43%), Gaps = 29/192 (15%)
Query: 9 VDMQRDFMP--GGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENH-------- 58
VD Q+ F P LPVP GD I +N+ + AL + ++D H
Sbjct: 12 VDAQKGFTPLCPDELPVPGGDAIGGALNQ----LASRAALRLGSKDAHSPKAAWVVESAE 67
Query: 59 -----ISFKERGGPWPKHCVQNTPGAEFVVDLPEDT---IIISKATEPDKEAYSGF---- 106
+++ + W HCV TPG E + +LP + K EPD Y
Sbjct: 68 QMLRPLAYPDADLTWVSHCVPGTPGFELLDELPRPIDYDFFVWKGVEPDLHPYGACYHDL 127
Query: 107 ---EGTNLDKILKEKGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDE 163
T + + L++ GV V V G+A +YCV+ TAL + GFEV L A + I +
Sbjct: 128 AERRSTGVIEYLRQNGVSHVLVGGLALDYCVKNTALQLRRAGFEVLLYLPACRAIAEDTA 187
Query: 164 EKALKEMEEKGI 175
++A EM + G+
Sbjct: 188 QRACDEMRDAGV 199
>pir||C72463 hypothetical protein APE2350 - Aeropyrum pernix (strain K1)
>gi|5106052|dbj|BAA81363.1| (AP000064) 231aa long
hypothetical protein [Aeropyrum pernix]
Length = 231
Score = 75.7 bits (183), Expect = 3e-13
Identities = 53/173 (30%), Positives = 79/173 (45%), Gaps = 16/173 (9%)
Query: 3 EEALIVVDMQRDFM-PGGALPVPEGDKIIPKVNEYIKKFREKGALIVATRDWHPENHISF 61
E A++VVDMQ DF+ P G L VP I + ++K R G + T+D H E F
Sbjct: 49 ETAVVVVDMQNDFVKPQGKLFVPTAPATIEPIRRLLEKARSAGVRVFYTQDTHYEGDPEF 108
Query: 62 KERGGPWPKHCVQNTPGAEFVVDLP----EDTIIISKATEPDKEAYSGFEGTNLDKILKE 117
+ W +H T G V +L D +++ K Y GF GT LD +L+
Sbjct: 109 EI----WGEHVRYGTWGWRIVEELKPVEGRDIVVM-------KTRYDGFYGTMLDDLLRV 157
Query: 118 KGVKRVYVCGVATEYCVRATALDALKHGFEVYLLRDAVKGIKPEDEEKALKEM 170
GVK + + G CV TA A ++V + D + + D AL+++
Sbjct: 158 YGVKNLVIVGTVANICVLHTAGSAALRWYKVVVPLDGISALNDFDYHAALRQI 210
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.316 0.139 0.410
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73100000
Number of Sequences: 2977
Number of extensions: 3221547
Number of successful extensions: 8111
Number of sequences better than 1.0e-10: 26
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 8047
Number of HSP's gapped (non-prelim): 29
length of query: 180
length of database: 189,106,746
effective HSP length: 53
effective length of query: 127
effective length of database: 157,386,935
effective search space: 19988140745
effective search space used: 19988140745
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 162 (67.5 bits)