BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1765 (ubiE-like) DE:MENAQUINONE BIOSYNTHESIS
METHLYTRANSFERASE related
(248 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G75136 menaquinone biosynthesis methlytransferase related P... 510 e-144
pir||A71001 hypothetical protein PH1305 - Pyrococcus horikoshii ... 384 e-106
pir||B72316 conserved hypothetical protein - Thermotoga maritima... 103 2e-21
pir||C71246 hypothetical protein PH0226 - Pyrococcus horikoshii ... 87 3e-16
pir||C75210 sterol biosynthesis methyltransferase related PAB221... 86 3e-16
emb|CAA70016.1| (Y08763) methyltransferase [Streptomyces griseus] 85 1e-15
emb|CAC14363.1| (AL445963) putative methyltransferase [Streptomy... 76 4e-13
sp|P54458|YQEM_BACSU HYPOTHETICAL 28.3 KD PROTEIN IN AROD-COMER ... 76 5e-13
gb|AAB84073.1| (U77459) EryCVI [Saccharopolyspora erythraea] >gi... 72 6e-12
dbj|BAB07674.1| (AP001520) unknown conserved protein [Bacillus h... 71 1e-11
gb|AAG13912.1|AF263245_8 (AF263245) daunosaminyl-N,N-dimethyltra... 69 4e-11
dbj|BAB06606.1| (AP001517) unknown conserved protein in others [... 68 9e-11
>pir||G75136 menaquinone biosynthesis methlytransferase related PAB1765 -
Pyrococcus abyssi (strain Orsay)
>gi|5458319|emb|CAB49808.1| (AJ248285) MENAQUINONE
BIOSYNTHESIS METHLYTRANSFERASE related [Pyrococcus
abyssi]
Length = 248
Score = 510 bits (1299), Expect = e-144
Identities = 248/248 (100%), Positives = 248/248 (100%)
Query: 1 MHELYTVLAEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELA 60
MHELYTVLAEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELA
Sbjct: 1 MHELYTVLAEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELA 60
Query: 61 KRGYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFD 120
KRGYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFD
Sbjct: 61 KRGYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFD 120
Query: 121 DYSIQQLFNSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVITDWREIEPAF 180
DYSIQQLFNSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVITDWREIEPAF
Sbjct: 121 DYSIQQLFNSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVITDWREIEPAF 180
Query: 181 QKMRFKRLVQIIKPDGSVKTFMVDDELNIYTPREMRLLAEKHFRKVKIYGNLHELKPNDR 240
QKMRFKRLVQIIKPDGSVKTFMVDDELNIYTPREMRLLAEKHFRKVKIYGNLHELKPNDR
Sbjct: 181 QKMRFKRLVQIIKPDGSVKTFMVDDELNIYTPREMRLLAEKHFRKVKIYGNLHELKPNDR 240
Query: 241 RYWLVAIK 248
RYWLVAIK
Sbjct: 241 RYWLVAIK 248
>pir||A71001 hypothetical protein PH1305 - Pyrococcus horikoshii
>gi|3257726|dbj|BAA30409.1| (AP000006) 252aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 252
Score = 384 bits (975), Expect = e-106
Identities = 171/248 (68%), Positives = 218/248 (86%), Gaps = 1/248 (0%)
Query: 1 MHELYTVLAEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELA 60
M+ELYT+LAEYYD IYR+R E VK EIDFVE++F+ +A+REV+R+LDLACGTG PTLELA
Sbjct: 1 MYELYTLLAEYYDTIYRRRIERVKAEIDFVEEIFKEDAKREVRRVLDLACGTGIPTLELA 60
Query: 61 KRGYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFD 120
+RGYEV+GLDLHEEML+VARRK+++ +K+EF+QG+ LEI F+ EFDA+TMFFS+I YFD
Sbjct: 61 ERGYEVVGLDLHEEMLRVARRKAKERNLKIEFLQGDVLEIAFKNEFDAVTMFFSTIMYFD 120
Query: 121 DYSIQQLFNSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVITDWREIEPAF 180
+ +++LF+ + +ALKPGG+F+ DFPCWFY G+D P++W+EQ G+E+LVI DWRE+EPA
Sbjct: 121 EEDLRKLFSKVAEALKPGGVFITDFPCWFYGGRDGPVVWNEQKGEEKLVIMDWREVEPAV 180
Query: 181 QKMRFKRLVQIIKPDGSVKTFMVDDELNIYTPREMRLLAEKHFRKVKIYGNL-HELKPND 239
QK+RFKRLVQI++P+G VK F+VDDELNIYTPRE+RLLAEK+F KVKIYGNL EL PND
Sbjct: 181 QKLRFKRLVQILRPNGEVKAFLVDDELNIYTPREVRLLAEKYFEKVKIYGNLKRELSPND 240
Query: 240 RRYWLVAI 247
RYW+V I
Sbjct: 241 MRYWIVGI 248
>pir||B72316 conserved hypothetical protein - Thermotoga maritima (strain MSB8)
>gi|4981476|gb|AAD36019.1|AE001757_12 (AE001757)
conserved hypothetical protein [Thermotoga maritima]
Length = 254
Score = 103 bits (254), Expect = 2e-21
Identities = 46/103 (44%), Positives = 75/103 (72%), Gaps = 1/103 (0%)
Query: 43 KRILDLACGTGTPTLELAKRGYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDF 102
K++LD+ACG GT +E+AK+G++V+G+DL EML+ AR+++++E I V F++ + E+DF
Sbjct: 40 KKVLDVACGEGTFAVEIAKQGFKVVGVDLSSEMLKFARKRAKEESISVVFLKKDMRELDF 99
Query: 103 EEEFDAITMFFSSITYFDDY-SIQQLFNSIKQALKPGGIFVAD 144
EEFD +T +F S+ Y DY +++ F + +ALK GG F+ D
Sbjct: 100 HEEFDIVTCWFDSLNYLLDYKDLKKTFEKVHEALKTGGAFLFD 142
>pir||C71246 hypothetical protein PH0226 - Pyrococcus horikoshii
>gi|3256615|dbj|BAA29298.1| (AP000001) 227aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 227
Score = 86.6 bits (211), Expect = 3e-16
Identities = 61/235 (25%), Positives = 110/235 (45%), Gaps = 42/235 (17%)
Query: 3 ELYTVLAEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELAKR 62
E Y V Y D+ + + I+ +E L + ++ K +LDLACG G + L
Sbjct: 5 EYYRVFPTYTDI----NSQEYRSRIETLEPLLMKYMKKRGK-VLDLACGVGGFSFLLEDY 59
Query: 63 GYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFDDY 122
G+EV+G+D+ E+M++ AR ++ VEFI G+A ++ FE++ +F SI +F+
Sbjct: 60 GFEVVGVDISEDMIRKAREYAKSRESNVEFIVGDARKLSFEDKTFDYVIFIDSIVHFEPL 119
Query: 123 SIQQLFNSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVITDWREIEPAFQK 182
+ Q+F +++ LKP G F+ F TD RE+ P ++
Sbjct: 120 ELNQVFKEVRRVLKPSGKFIMYF-------------------------TDLRELLPRLKE 154
Query: 183 ---MRFKRLVQIIKPDGSVKTFMVDDE---------LNIYTPREMRLLAEKHFRK 225
+ K + + PD +T +++ + N++ + LLA+ +F K
Sbjct: 155 SLVVGQKYWISKVIPDQEERTVVIEFKSEQDSFRVRFNVWGKTGVELLAKLYFTK 209
>pir||C75210 sterol biosynthesis methyltransferase related PAB2213 - Pyrococcus
abyssi (strain Orsay) >gi|5457639|emb|CAB49130.1|
(AJ248283) sterol biosynthesis methyltransferase related
[Pyrococcus abyssi]
Length = 227
Score = 86.2 bits (210), Expect = 3e-16
Identities = 63/235 (26%), Positives = 110/235 (46%), Gaps = 42/235 (17%)
Query: 3 ELYTVLAEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELAKR 62
E Y Y D+ ++ ++ ++ +E L + +R K +LDLACG G + L
Sbjct: 5 EYYRAFPTYTDINSQEYRD----RLENLEPLLMKYMKRRGK-VLDLACGVGGFSFLLEDY 59
Query: 63 GYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFDDY 122
G+EV+GLD+ EEM+ A+ ++++ VEFI G+A ++ FE+ +F S+ +F
Sbjct: 60 GFEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPL 119
Query: 123 SIQQLFNSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVITDWREIEPAFQK 182
+ Q+F +K+ LKP G F+ F TD RE+ P ++
Sbjct: 120 ELNQVFKEVKRVLKPTGKFIIYF-------------------------TDMRELLPRLRE 154
Query: 183 ---MRFKRLVQIIKPDGSVKTFMVDDE---------LNIYTPREMRLLAEKHFRK 225
+ K + I PD KT +++ + N++ + LLA+ +F K
Sbjct: 155 SLVVGEKYWINKIIPDQEEKTVVIEFKSEENSFRVRFNVWGKTGVELLAKLYFNK 209
>emb|CAA70016.1| (Y08763) methyltransferase [Streptomyces griseus]
Length = 253
Score = 84.7 bits (206), Expect = 1e-15
Identities = 44/125 (35%), Positives = 69/125 (55%), Gaps = 8/125 (6%)
Query: 28 DFVEDLFRREAEREVKR--------ILDLACGTGTPTLELAKRGYEVIGLDLHEEMLQVA 79
D +L +EA V+ +LD CG G LA RGY V+G+D E L +A
Sbjct: 25 DLTPELSEKEASNMVELGGFDAGMDLLDAPCGHGRHANVLASRGYRVVGVDRDERFLSMA 84
Query: 80 RRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFDDYSIQQLFNSIKQALKPGG 139
R+++E G++V++ + E+ F EFDA ++SS YFDD + + + ++AL+PGG
Sbjct: 85 RKEAESMGVQVDYRHVDLREMSFSAEFDAAVSWYSSFGYFDDETDRDILRRYRRALRPGG 144
Query: 140 IFVAD 144
F+ D
Sbjct: 145 RFLLD 149
>emb|CAC14363.1| (AL445963) putative methyltransferase [Streptomyces coelicolor]
Length = 249
Score = 76.1 bits (184), Expect = 4e-13
Identities = 59/228 (25%), Positives = 111/228 (47%), Gaps = 22/228 (9%)
Query: 10 EYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELAKRGYEVIGL 69
E++D ++ +++ +E+ L A R+LDL CG G T+ LA+RGY+V G+
Sbjct: 15 EFHDFLFSEQRHAQAEELLDTSPLLSIPAGA---RVLDLCCGPGVFTVPLARRGYDVTGV 71
Query: 70 DLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFDDYSIQ-QLF 128
DL ML+ AR+++ G +V ++Q +A + FD + F+S YF++ + ++
Sbjct: 72 DLSPAMLERARKRAADAGAQVTYVQADARAYEPPGAFDVVLNMFTSFGYFENPADNARVL 131
Query: 129 NSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVIT-----DWREIEPAFQKM 183
++ L PGG V D + Q G++ +V T DW + + +
Sbjct: 132 RTMYACLAPGGTLVLDLAGKELLARKVTPPKVVQRGEDLMVQTDTVLDDWARLRSDWVLV 191
Query: 184 RFKRLVQIIKPDGSVKTFMVDDELNIYTPREM-RLLAEKHFRKVKIYG 230
R +R+ + ++ F +Y+ E+ R++ E F +V+I+G
Sbjct: 192 RGERVTR-----ATLVWF-------VYSAVELRRMVEEAGFGQVEIFG 227
>sp|P54458|YQEM_BACSU HYPOTHETICAL 28.3 KD PROTEIN IN AROD-COMER INTERGENIC REGION
>gi|7474691|pir||A69952 conserved hypothetical protein
yqeM - Bacillus subtilis >gi|1303794|dbj|BAA12450.1|
(D84432) YqeM [Bacillus subtilis]
>gi|2635007|emb|CAB14503.1| (Z99117) similar to
hypothetical proteins [Bacillus subtilis]
Length = 247
Score = 75.7 bits (183), Expect = 5e-13
Identities = 51/144 (35%), Positives = 78/144 (53%), Gaps = 15/144 (10%)
Query: 41 EVKRILDLACGTGTPTLELAKRGYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEI 100
E RILDLACGTG ++ LA++G+EV G+DL EEML A++K + F+Q + EI
Sbjct: 32 EKGRILDLACGTGEISIRLAEKGFEVTGIDLSEEMLSFAQQKVSSSQ-PILFLQQDMREI 90
Query: 101 -DFEEEFDAITMFFSSITYFDDYS-IQQLFNSIKQALKPGGIFVAD----------FPCW 148
F+ +FDA+ + S+ Y + + + F S+ + LKP GI + D FP
Sbjct: 91 TGFDGQFDAVVICCDSLNYLKTKNDVIETFKSVFRVLKPEGILLFDVHSSFKIAEVFPDS 150
Query: 149 FYSGKDYPI--IWDEQNGDERLVI 170
++ +D I IW G + L +
Sbjct: 151 TFADQDEDISYIWQSFAGSDELSV 174
>gb|AAB84073.1| (U77459) EryCVI [Saccharopolyspora erythraea]
>gi|2665337|emb|CAA72082.1| (Y11199) putative
TDP-N-dimethyldesosamine-N-methyltransferase
[Saccharopolyspora erythraea]
Length = 237
Score = 72.2 bits (174), Expect = 6e-12
Identities = 53/142 (37%), Positives = 71/142 (49%), Gaps = 7/142 (4%)
Query: 9 AEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELAKRGYEVIG 68
AE YD YR R + E V L R +LD+ACGTGT A +V G
Sbjct: 7 AELYDRFYRGRGKDYAAEAAQVARLVRDRLP-SASSLLDVACGTGTHLRRFADLFDDVTG 65
Query: 69 LDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYF-DDYSIQQL 127
L+L M++VAR + GI V +QG+ + + EFDA+T FSSI + D + Q
Sbjct: 66 LELSAAMIEVAR--PQLGGIPV--LQGDMRDFALDREFDAVTCMFSSIGHMRDGAELDQA 121
Query: 128 FNSIKQALKPGGIFVADFPCWF 149
S + L PGG+ V + P WF
Sbjct: 122 LASFARHLAPGGVVVVE-PWWF 142
>dbj|BAB07674.1| (AP001520) unknown conserved protein [Bacillus halodurans]
Length = 255
Score = 71.0 bits (171), Expect = 1e-11
Identities = 52/199 (26%), Positives = 99/199 (49%), Gaps = 9/199 (4%)
Query: 45 ILDLACGTGTPTLELAKRGYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEE 104
I+DLACGTG T+ LA +GY+++G+D+H+ ML+ AR KS + + +E+I+ + ++
Sbjct: 39 IVDLACGTGRATIPLASKGYKLMGVDVHKGMLEAAREKSSRLNLPIEWIKQDCTKLSLNL 98
Query: 105 EFDAITMFFSSITYFDDYSIQQLF-NSIKQALKPGGIFVAD--FP----CWFYSGKDYPI 157
I + +F Q F S+ + LK GIF+ D FP + ++Y
Sbjct: 99 MSPFIYSVGNVFQHFLTNEEQDSFLTSVNKHLKESGIFIFDTRFPSKEELLQPNTEEYWK 158
Query: 158 IWDEQNGDERLVITDWREIEPAFQKMRFKRLVQIIKPDG-SVKTFMVDDELNIYTPREM- 215
+ + + +++ + + +P Q + + + + +G +V + L P+EM
Sbjct: 159 SYQDTDTGQKVDVYTISQYDPLTQIQHYTMIRKSVNAEGLAVNEKRTNIRLRYVFPKEME 218
Query: 216 RLLAEKHFRKVKIYGNLHE 234
RLL+ F + +Y + E
Sbjct: 219 RLLSANGFEIIDVYQDWQE 237
>gb|AAG13912.1|AF263245_8 (AF263245) daunosaminyl-N,N-dimethyltransferase [Micromonospora
megalomicea subsp. nigra]
Length = 257
Score = 69.5 bits (167), Expect = 4e-11
Identities = 45/142 (31%), Positives = 69/142 (47%), Gaps = 7/142 (4%)
Query: 9 AEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELAKRGYEVIG 68
A+ Y Y R + + E D + ++ R+ + +LD+ACGTG+ +ELA EV+G
Sbjct: 19 ADIYHDFYHGRGKGYRAEADALVEVARKHTP-QAATLLDVACGTGSHLVELADSFREVVG 77
Query: 69 LDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYF-DDYSIQQL 127
+DL ML A R E QG+ + + FD +T FSS Y D+ + +
Sbjct: 78 VDLSAAMLATAARNDPGR----ELHQGDMRDFSLDRRFDVVTCMFSSTGYLVDEAELDRA 133
Query: 128 FNSIKQALKPGGIFVADFPCWF 149
++ L PGG V + P WF
Sbjct: 134 VANLAGHLAPGGTLVVE-PWWF 154
>dbj|BAB06606.1| (AP001517) unknown conserved protein in others [Bacillus
halodurans]
Length = 261
Score = 68.3 bits (164), Expect = 9e-11
Identities = 45/131 (34%), Positives = 71/131 (53%), Gaps = 10/131 (7%)
Query: 15 IYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELAKRGYEVIGLDLHEE 74
+Y +R V + EDL + A +E + +LDL CGTG T ++ + G VIG+D+ E
Sbjct: 17 LYDERHRFVSA---YGEDLIQWLAPKEGECVLDLGCGTGDLTEQIHQLGSRVIGVDVSES 73
Query: 75 MLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFDDYSIQQLFNSIKQA 134
M++ A+ K ++F A ++ F E+FDAI FS+ ++ I ++
Sbjct: 74 MIEQAKGKFP----HLDFQVAEATDLSFSEKFDAI---FSNAVLHWIKDAEEALTVIYRS 126
Query: 135 LKPGGIFVADF 145
LKPGG FVA+F
Sbjct: 127 LKPGGRFVAEF 137
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.323 0.142 0.422
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94646854
Number of Sequences: 2977
Number of extensions: 3961434
Number of successful extensions: 11379
Number of sequences better than 1.0e-10: 12
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 11365
Number of HSP's gapped (non-prelim): 12
length of query: 248
length of database: 189,106,746
effective HSP length: 52
effective length of query: 196
effective length of database: 157,985,422
effective search space: 30965142712
effective search space used: 30965142712
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 164 (68.3 bits)