BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1786 (hydD-1) DE:CYTOCHROME-C3 HYDROGENASE DELTA CHAIN
(261 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75133 cytochrome-c3 hydrogenase delta chain PAB1786 - Pyro... 549 e-155
pir||A71075 probable cytochrome-c3 hydrogenase delta chain - Pyr... 522 e-147
pir||S48835 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain ... 513 e-145
gb|AAB94935.1| (AF039208) NiFe hydrogenase delta subunit [Thermo... 449 e-125
pir||A75070 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain ... 182 5e-45
gb|AAF61853.1|AF176650_3 (AF176650) sulfhydrogenase II subunit d... 178 8e-44
pir||F64448 methylviologen-reducing hydrogenase gamma chain homo... 123 3e-27
pir||S24800 methylviologen-reducing hydrogenase chain vhuG - Met... 123 3e-27
pir||C59304 methylviologen-reducing hydrogenase gamma chain homo... 122 4e-27
gb|AAB85624.1| (AE000883) methyl viologen-reducing hydrogenase, ... 90 3e-17
pir||C30315 coenzyme-M-7-mercaptoheptanoylthreonine-phosphate-he... 89 6e-17
gi|11498969 methylviologen-reducing hydrogenase, subunit gamma (... 87 2e-16
pir||D30315 methyl viologen-reducing hydrogenase gamma chain - M... 85 6e-16
gb|AAB02350.1| (J04540) mvhG gene product [Methanobacterium ther... 85 1e-15
sp|P22319|HOXY_ALCEU NAD-REDUCING HYDROGENASE HOXS DELTA SUBUNIT... 83 4e-15
pir||S32834 methylviologen-reducing hydrogenase chain vhcG [simi... 82 7e-15
pir||S16726 methylviologen-reducing hydrogenase chain vhcG - Met... 81 2e-14
gb|AAB57891.1| (U70364) NAD-reducing hydrogenase delta subunit [... 80 4e-14
emb|CAA56463.1| (X80180) hydrogenase small beta subunit [Acetomi... 76 4e-13
sp|Q60340|FRHG_METJA COENZYME F420 HYDROGENASE GAMMA SUBUNIT (8-... 74 2e-12
>pir||C75133 cytochrome-c3 hydrogenase delta chain PAB1786 - Pyrococcus abyssi
(strain Orsay) >gi|5458291|emb|CAB49780.1| (AJ248285)
CYTOCHROME-C3 HYDROGENASE DELTA CHAIN [Pyrococcus
abyssi]
Length = 261
Score = 549 bits (1400), Expect = e-155
Identities = 261/261 (100%), Positives = 261/261 (100%)
Query: 1 MGKLRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGS 60
MGKLRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGS
Sbjct: 1 MGKLRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGS 60
Query: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKKA 120
VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKKA
Sbjct: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKKA 120
Query: 121 EPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKG 180
EPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKG
Sbjct: 121 EPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKG 180
Query: 181 EPCLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMKI 240
EPCLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMKI
Sbjct: 181 EPCLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMKI 240
Query: 241 FNGHDERIEKMVEKVFQEVKE 261
FNGHDERIEKMVEKVFQEVKE
Sbjct: 241 FNGHDERIEKMVEKVFQEVKE 261
>pir||A71075 probable cytochrome-c3 hydrogenase delta chain - Pyrococcus
horikoshii >gi|3257712|dbj|BAA30395.1| (AP000005) 266aa
long hypothetical cytochrome-c3 hydrogenase delta chain
[Pyrococcus horikoshii]
Length = 266
Score = 522 bits (1331), Expect = e-147
Identities = 241/259 (93%), Positives = 256/259 (98%)
Query: 3 KLRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGSVS 62
K+RIGFYALTSCYGCQLQLAMMDELL L+P+ E+VCWYM+DRDS++D+PVDIAFIEGSVS
Sbjct: 8 KIRIGFYALTSCYGCQLQLAMMDELLLLLPHIELVCWYMVDRDSIDDEPVDIAFIEGSVS 67
Query: 63 TEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKKAEP 122
TEEEVELVKKIREN+KIVVAVGACAVQGGVQSWTDKSLEELW+TVYGDAKVKF+PKKAEP
Sbjct: 68 TEEEVELVKKIRENSKIVVAVGACAVQGGVQSWTDKSLEELWRTVYGDAKVKFKPKKAEP 127
Query: 123 VSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKGEP 182
VSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKGEP
Sbjct: 128 VSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKGEP 187
Query: 183 CLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMKIFN 242
CLGP+TRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEI+ERMKIFN
Sbjct: 188 CLGPVTRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEIIERMKIFN 247
Query: 243 GHDERIEKMVEKVFQEVKE 261
GHD+RIEKMVEK+FQ VKE
Sbjct: 248 GHDDRIEKMVEKIFQGVKE 266
>pir||S48835 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain - Pyrococcus
furiosus >gi|562776|emb|CAA53036.1| (X75255) hydrogenase
(delta subunit) [Pyrococcus furiosus]
Length = 261
Score = 513 bits (1308), Expect = e-145
Identities = 234/256 (91%), Positives = 253/256 (98%)
Query: 1 MGKLRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGS 60
MGK+RIGFYALTSCYGCQLQLAMMDELL+LIPNAEIVCW+M+DRDS+ED+ VDIAFIEGS
Sbjct: 1 MGKVRIGFYALTSCYGCQLQLAMMDELLQLIPNAEIVCWFMIDRDSIEDEKVDIAFIEGS 60
Query: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKKA 120
VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSW++K LEELWK VYGDAKVKFQPKKA
Sbjct: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWSEKPLEELWKKVYGDAKVKFQPKKA 120
Query: 121 EPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKG 180
EPVSKYIKVDYNIYGCPPEK+DFLYALGTFLIGSWPEDIDYPVCLECRLNG+PC+LLEKG
Sbjct: 121 EPVSKYIKVDYNIYGCPPEKKDFLYALGTFLIGSWPEDIDYPVCLECRLNGHPCILLEKG 180
Query: 181 EPCLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMKI 240
EPCLGP+TRAGCNARCPGFG+ACIGCRGAIGYDVAWFDSLA+VFKEKG+TKEEI+ERMK+
Sbjct: 181 EPCLGPVTRAGCNARCPGFGVACIGCRGAIGYDVAWFDSLAKVFKEKGMTKEEIIERMKM 240
Query: 241 FNGHDERIEKMVEKVF 256
FNGHDER+EKMVEK+F
Sbjct: 241 FNGHDERVEKMVEKIF 256
>gb|AAB94935.1| (AF039208) NiFe hydrogenase delta subunit [Thermococcus litoralis]
Length = 263
Score = 449 bits (1143), Expect = e-125
Identities = 205/257 (79%), Positives = 231/257 (89%), Gaps = 1/257 (0%)
Query: 3 KLRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGSVS 62
K+RIGFYALTSCYGCQLQ AMMDE+L L+ AEI CW+M++RDS ED+ VDIAFIEGSVS
Sbjct: 5 KVRIGFYALTSCYGCQLQFAMMDEILHLLDKAEIECWFMVERDSDEDREVDIAFIEGSVS 64
Query: 63 TEEEVELVKKIRENAKIVVAVGACAVQGGVQSW-TDKSLEELWKTVYGDAKVKFQPKKAE 121
T+EEVELVKKIRE AKIVVAVGACA QGGVQSW DK L ELWKTVYGDA VKF+PK AE
Sbjct: 65 TQEEVELVKKIREKAKIVVAVGACATQGGVQSWGKDKELSELWKTVYGDAHVKFEPKMAE 124
Query: 122 PVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKGE 181
PV KYIKVDY +YGCPPEK+DFLYALGTFL+GSWPEDIDYPVC+ECRL G PC+L+EKGE
Sbjct: 125 PVEKYIKVDYKLYGCPPEKKDFLYALGTFLVGSWPEDIDYPVCVECRLRGNPCILIEKGE 184
Query: 182 PCLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMKIF 241
PCLGP+T AGC+ARCPGF +ACIGCRGA+GYDVAWFDSLA FK+KGLTKEEILERMKIF
Sbjct: 185 PCLGPVTVAGCDARCPGFNVACIGCRGAVGYDVAWFDSLALEFKKKGLTKEEILERMKIF 244
Query: 242 NGHDERIEKMVEKVFQE 258
N H+ ++E+MV K+F+E
Sbjct: 245 NAHNPKLEEMVNKIFEE 261
>pir||A75070 cytochrome-c3 hydrogenase (EC 1.12.2.1) delta chain PAB0640 -
Pyrococcus abyssi (strain Orsay)
>gi|5458374|emb|CAB49862.1| (AJ248286) HYDROGENASE (EC
1.12.2.1) (CYTOCHROME-C3 HYDROGENASE DELTA CHAIN)
[Pyrococcus abyssi]
Length = 241
Score = 182 bits (456), Expect = 5e-45
Identities = 95/242 (39%), Positives = 143/242 (58%), Gaps = 5/242 (2%)
Query: 1 MGKLRIGFYALTSCYGCQLQLAMMDE-LLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEG 59
M KL++G + LTSC GC L + + E L ++ +I ++M +K +D+A + G
Sbjct: 1 MDKLKLGVFELTSCGGCALNILFLYERLFDILEFYDIAEFHMATSQRGREK-LDVALVTG 59
Query: 60 SVSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKK 119
SVST+ ++E+VK R A+ ++A+G CA GGVQ + S++E + +YGD K + +
Sbjct: 60 SVSTQRDLEVVKDARNRAEYLIALGTCATHGGVQGSIEGSVKEGLRKIYGDMKGPSKVLE 119
Query: 120 AEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEK 179
V +++ VD+ I GCP +K + L G P DYPVCLEC+LN Y CVLL++
Sbjct: 120 PRAVVEHVPVDFAIPGCPYDKDEVFQVLMDIARGVEPVTKDYPVCLECKLNEYECVLLKR 179
Query: 180 GEPCLGPITRAGCNARCPGFGIACIGCRGAIGYDVAWFDSLARVFKEKGLTKEEILERMK 239
G PCLGP+T GCNA+CP G+ CIGCRG + L V K + +E+I+ ++K
Sbjct: 180 GVPCLGPVTLGGCNAKCPSIGLGCIGCRGLV--PDPNIPGLVEVLKNI-IPEEDIVRKLK 236
Query: 240 IF 241
F
Sbjct: 237 TF 238
>gb|AAF61853.1|AF176650_3 (AF176650) sulfhydrogenase II subunit d [Pyrococcus furiosus]
Length = 237
Score = 178 bits (446), Expect = 8e-44
Identities = 94/240 (39%), Positives = 144/240 (59%), Gaps = 8/240 (3%)
Query: 4 LRIGFYALTSCYGCQLQLAMM-DELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGSVS 62
+++G + LT C GC L L + D+LL L+ EI ++M +K +D+A + G+VS
Sbjct: 1 MKLGVFELTDCGGCALNLLFLYDKLLDLLEFYEIAEFHMATSKKSREK-IDVALVTGTVS 59
Query: 63 TEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKKAEP 122
T+ ++E+++ R ++ ++A+G CA G VQ + S +E ++ VYG+ K + +P
Sbjct: 60 TQRDLEVLRDARNRSEYLIALGTCATHGSVQGVIENS-KEAYRRVYGNGKPPVKLLNPKP 118
Query: 123 VSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKGEP 182
V+ Y+ VD+ I GCP +K + L G P DYPVCLEC+LN Y CVLL+K P
Sbjct: 119 VTDYVPVDFAIPGCPYDKEEVFQVLIDIAKGIEPVAKDYPVCLECKLNEYECVLLKKRIP 178
Query: 183 CLGPITRAGCNARCPGFGIACIGCRG-AIGYDVAWFDSLARVFKEKGLTKEEILERMKIF 241
CLGP+T GCNA+CP +G+ CIGCRG ++ +V + V KE L EEI +++ F
Sbjct: 179 CLGPVTAGGCNAKCPSYGLGCIGCRGPSLDNNV---PGMFEVLKEI-LPDEEIARKLRTF 234
>pir||F64448 methylviologen-reducing hydrogenase gamma chain homolog -
Methanococcus jannaschii >gi|1591819|gb|AAB99193.1|
(U67560) methylviologen-reducing hydrogenase, gamma
chain (vhuG) [Methanococcus jannaschii]
Length = 288
Score = 123 bits (305), Expect = 3e-27
Identities = 85/269 (31%), Positives = 126/269 (46%), Gaps = 30/269 (11%)
Query: 4 LRIGFYALTSCYGCQLQLA-MMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGSVS 62
+++G L C GC + L + D+LL+++PN EIV ++ + +D+ +EG +
Sbjct: 6 VKVGMIQLCGCSGCHISLLDLHDKLLEVLPNLEIVYAPIIADPKEIPEGIDVFLVEGGIR 65
Query: 63 TEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDK-SLEELWKTVY--------GDAKV 113
E + L+ +IRE +KIV+A G CA GG+ + EEL VY G+
Sbjct: 66 NEHDEHLIHEIREKSKIVIAWGTCAAYGGIPGLGNLYKKEELLNYVYSTDSTENKGEIPS 125
Query: 114 KFQPKKAE---PVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLEC--- 167
+ P E P+ +IKVDY I GCPP + A+ L G P+ VC EC
Sbjct: 126 EEIPPLEEYVKPIKDFIKVDYTIPGCPPTPKMIADAIIALLNGEEPKLPTKIVCDECPRK 185
Query: 168 RLNGYPCV--------------LLEKGEPCLGPITRAGCNARCPGFGIACIGCRGAIGYD 213
+ N +P L E+G CLG TRAGC A+CP G+ C GC G
Sbjct: 186 KENVFPETFKRTHEGRPDPERCLFEQGYTCLGFATRAGCGAKCPSAGVPCRGCFGKTDKS 245
Query: 214 VAWFDSLARVFKEKGLTKEEILERMKIFN 242
+ + A V G EI +++ + N
Sbjct: 246 LDLGANAANVLANAGEAALEIPDKVALLN 274
>pir||S24800 methylviologen-reducing hydrogenase chain vhuG - Methanococcus
voltae
Length = 287
Score = 123 bits (305), Expect = 3e-27
Identities = 90/271 (33%), Positives = 122/271 (44%), Gaps = 32/271 (11%)
Query: 3 KLRIGFYALTSCYGCQLQLA-MMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGSV 61
K+R+G L C GC + L + ++LL ++PN EIV + + D E D+ IEG
Sbjct: 4 KVRLGLIQLCGCSGCHISLLDLHEQLLDVLPNLEIV-YAPIIADVKEIPECDVFLIEGGA 62
Query: 62 STEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDK-SLEELWKTVYG----------- 109
E + L+ +IRE +K+V+A G CAV GG+ + S E+L KTVYG
Sbjct: 63 RNEHDEHLIHEIREKSKVVIAWGTCAVYGGIPGLGNLYSAEQLKKTVYGTETTDNVGELP 122
Query: 110 -DAKVKFQPKKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLEC- 167
D V PV + V+Y I GCPP A+ L G PE VC EC
Sbjct: 123 SDEMVPPLTNSVMPVPSIVDVEYVIPGCPPRPEINAGAIVALLEGRDPELPQKIVCDECP 182
Query: 168 -------------RLNGYPC---VLLEKGEPCLGPITRAGCNARCPGFGIACIGCRGAIG 211
G P L E+G C+G TRAGC A CP G+ C GC G
Sbjct: 183 RTKENVIPETFKRTFEGTPDNEKCLFEQGYTCVGMGTRAGCGALCPSAGVPCRGCYGKTD 242
Query: 212 YDVAWFDSLARVFKEKGLTKEEILERMKIFN 242
+ SLA + G +I ++ +FN
Sbjct: 243 EVLDQGSSLANTYAAAGDEALKISDKSALFN 273
>pir||C59304 methylviologen-reducing hydrogenase gamma chain homolog vhuG
[similarity] - Methanococcus voltae
>gi|1747407|emb|CAA43509.1| (X61204)
methylviologen-reducing hydrogenase subunit
[Methanococcus voltae]
Length = 287
Score = 122 bits (304), Expect = 4e-27
Identities = 90/271 (33%), Positives = 122/271 (44%), Gaps = 32/271 (11%)
Query: 3 KLRIGFYALTSCYGCQLQLA-MMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGSV 61
K+R+G L C GC + L + ++LL ++PN EIV + + D E D+ IEG
Sbjct: 4 KVRLGLIQLCGCSGCHISLLDLHEQLLDVLPNLEIV-YAPIIADVKEIPECDVFLIEGGA 62
Query: 62 STEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDK-SLEELWKTVYG----------- 109
E + L+ +IRE +K+V+A G CAV GG+ + S E+L KTVYG
Sbjct: 63 RNEHDEHLIHEIREKSKVVIAWGTCAVYGGIPGLGNLYSAEQLKKTVYGTETTDNVGELP 122
Query: 110 -DAKVKFQPKKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLEC- 167
D V PV + V+Y I GCPP A+ L G PE VC EC
Sbjct: 123 SDEMVPPLTNSVMPVPSIVDVEYVIPGCPPRPEINAGAIVALLEGRDPELPKKIVCDECP 182
Query: 168 -------------RLNGYPC---VLLEKGEPCLGPITRAGCNARCPGFGIACIGCRGAIG 211
G P L E+G C+G TRAGC A CP G+ C GC G
Sbjct: 183 RTKENVIPETFKRTFEGTPDPEKCLFEQGYTCVGMGTRAGCGALCPSAGVPCRGCYGKTD 242
Query: 212 YDVAWFDSLARVFKEKGLTKEEILERMKIFN 242
+ SLA + G +I ++ +FN
Sbjct: 243 EVLDQGSSLANTYAAAGDEALKISDKSALFN 273
>gb|AAB85624.1| (AE000883) methyl viologen-reducing hydrogenase, gamma subunit
[Methanobacterium thermoautotrophicum]
Length = 308
Score = 90.1 bits (220), Expect = 3e-17
Identities = 68/245 (27%), Positives = 108/245 (43%), Gaps = 42/245 (17%)
Query: 3 KLRIGFYALTSCYGCQLQLAMMDE-LLKLIPNAEIVCWYMLDRDSVEDKP-VDIAFIEGS 60
K++IG L C GC L +A E ++ ++ +A+ +L ++ P +D+ IEG
Sbjct: 4 KIKIGTMWLGGCSGCHLSIADFHEKIIDVMEHADFEFSPVLMDTKYDEIPELDVVIIEGG 63
Query: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQS----W-TDKSLEELW---------KT 106
+ +E E +++RE AK V++ G CAV GG+ W D+ +EE + +
Sbjct: 64 IVNDENREFAEELREKAKFVISYGTCAVYGGIPGLRNLWDKDEVIEEAYINSITTPNEEG 123
Query: 107 VYGDAKVKFQPKKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYP---V 163
V V + +P+ + I VD+ + GCPP A+ L G E+I+ P +
Sbjct: 124 VIPSEDVPHLEGRVKPLGEVIDVDFEVPGCPPRSDVAAEAVMALLTG---EEIELPETNL 180
Query: 164 CLECRLNGYP--------------------CVLLEKGEPCLGPITRAGCNARCPGFGIAC 203
C C P L+ +G C+GP T + C A CP I C
Sbjct: 181 CEVCPREKPPEGLAMDFIKRQFEVGKPEDDLCLIPQGLICMGPATVSICGAECPSIAIPC 240
Query: 204 IGCRG 208
GC G
Sbjct: 241 RGCYG 245
>pir||C30315 coenzyme-M-7-mercaptoheptanoylthreonine-phosphate-heterodisu lfide
hydrogenase (EC 1.12.99.2) 41K chain - Methanobacterium
thermoautotrophicum (strains Delta H and Marburg)
Length = 308
Score = 88.9 bits (217), Expect = 6e-17
Identities = 67/245 (27%), Positives = 108/245 (43%), Gaps = 42/245 (17%)
Query: 3 KLRIGFYALTSCYGCQLQLA-MMDELLKLIPNAEIVCWYMLDRDSVEDKP-VDIAFIEGS 60
K++IG L C GC L +A +++ ++ +A+ +L ++ P +D+ IEG
Sbjct: 4 KIKIGTMWLGGCSGCHLSIADFHGKIIDVMEHADFEFSPVLMDTKYDEIPELDVVIIEGG 63
Query: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQS----W-TDKSLEELW---------KT 106
+ +E E +++RE AK V++ G CAV GG+ W D+ +EE + +
Sbjct: 64 IVNDENREFAEELREKAKFVISYGTCAVYGGIPGLRNLWDKDEVIEEAYINSITTPNEEG 123
Query: 107 VYGDAKVKFQPKKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYP---V 163
V V + +P+ + I VD+ + GCPP A+ L G E+I+ P +
Sbjct: 124 VIPSEDVPHLEGRVKPLGEVIDVDFEVPGCPPRSDVAAEAVMALLTG---EEIELPETNL 180
Query: 164 CLECRLNGYP--------------------CVLLEKGEPCLGPITRAGCNARCPGFGIAC 203
C C P L+ +G C+GP T + C A CP I C
Sbjct: 181 CEVCPREKPPEGLAMDFIKRQFEVGKPEDDLCLIPQGLICMGPATVSICGAECPSIAIPC 240
Query: 204 IGCRG 208
GC G
Sbjct: 241 RGCYG 245
>gi|11498969 methylviologen-reducing hydrogenase, subunit gamma (vhuG)
[Archaeoglobus fulgidus] >gi|7483871|pir||D69421
methylviologen-reducing hydrogenase, subunit gamma
(vhuG) homolog - Archaeoglobus fulgidus
>gi|2649199|gb|AAB89871.1| (AE001009)
methylviologen-reducing hydrogenase, subunit gamma
(vhuG) [Archaeoglobus fulgidus]
Length = 293
Score = 87.4 bits (213), Expect = 2e-16
Identities = 71/254 (27%), Positives = 105/254 (40%), Gaps = 32/254 (12%)
Query: 6 IGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVEDKP-VDIAFIEGSVSTE 64
+ FY C GC + + + E + L + P VD AFIEG +
Sbjct: 4 VAFYIAHGCSGCTMSMLDLGERMNDFRMRVAWAAPTLGDGKYDRIPEVDYAFIEGGIGLS 63
Query: 65 EEVELVKKIRENAKIVVAVGACAVQGGVQSWTD-KSLEELWKTVYGDAK-------VKFQ 116
E VE+V+++RE ++ +VA G CA GG+ + S EE+ + V+G AK +
Sbjct: 64 EHVEIVRELREKSETLVAFGICASCGGIMGLANLHSKEEILEDVFGSAKSSVGDLRIPEM 123
Query: 117 PKKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIG--SWPEDIDYPVCLEC------- 167
+ +P+ ++VD I GCPP A G W VC C
Sbjct: 124 LEFVKPLDSVVEVDCYIGGCPPTPEQIYNAFKAIFEGERGWITS-GKSVCEFCSRNLSEK 182
Query: 168 --------RLNGYPCVLLEKGEPCLGPITRAGCNARCPGFGIACIGCRGAI----GYDVA 215
R+ C L++G C GP T+ C A CP C GC G + + A
Sbjct: 183 FGEVSRFLRVPDKEC-FLKQGILCFGPATQGDCMASCPNSNSPCRGCGGMLPSVDDHGSA 241
Query: 216 WFDSLARVFKEKGL 229
D L+ + EKG+
Sbjct: 242 LVDMLSSLTDEKGI 255
>pir||D30315 methyl viologen-reducing hydrogenase gamma chain - Methanobacterium
thermoautotrophicum (strain winter)
Length = 308
Score = 85.4 bits (208), Expect = 6e-16
Identities = 67/246 (27%), Positives = 104/246 (42%), Gaps = 46/246 (18%)
Query: 4 LRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCW----YMLDRDSVEDKPVDIAFIEG 59
++IG L C GC L +A + E L I E+ + ++D E +D+ IEG
Sbjct: 5 IKIGTMWLGGCSGCHLSIADLHEAL--IDVMELADFEFSPVLMDTKYDEIPELDVVIIEG 62
Query: 60 SVSTEEEVELVKKIRENAKIVVAVGACAVQGGVQS----W-TDKSLEELW---------K 105
+ +E E +++RE AK V++ G CAV GG+ W D+ +EE + +
Sbjct: 63 GIVNDENREFAEELREKAKFVISYGTCAVYGGIPGLRNLWDKDEVIEEAYINSITTPNEE 122
Query: 106 TVYGDAKVKFQPKKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYP--- 162
V V + +P+ + I VD+ + GCPP + L G E+++ P
Sbjct: 123 GVIPSEDVPHLEGRVKPLGEVIDVDFEVPGCPPRSDVVAEVVLALLKG---EEVELPQTN 179
Query: 163 VCLECRLNGYP--------------------CVLLEKGEPCLGPITRAGCNARCPGFGIA 202
+C C P L+ +G C+GP T + C A CP I
Sbjct: 180 LCEVCPREKPPEGLAMDFIKRQFEVGKPEDDLCLIPQGLICMGPATVSICGAECPSIAIP 239
Query: 203 CIGCRG 208
C GC G
Sbjct: 240 CRGCYG 245
>gb|AAB02350.1| (J04540) mvhG gene product [Methanobacterium thermoautotrophicum]
Length = 308
Score = 84.7 bits (206), Expect = 1e-15
Identities = 66/245 (26%), Positives = 107/245 (42%), Gaps = 42/245 (17%)
Query: 3 KLRIGFYALTSCYGCQLQLA-MMDELLKLIPNAEIVCWYMLDRDSVEDKP-VDIAFIEGS 60
K++IG L C GC L +A +++ ++ +A+ +L ++ P +D+ IEG
Sbjct: 4 KIKIGTMWLGGCSGCHLSIADFHGKIIDVMEHADFEFSPVLMDTKYDEIPELDVVIIEGG 63
Query: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQS----W-TDKSLEELW---------KT 106
+ +E E +++RE AK V++ G CAV GG+ W D+ +EE + +
Sbjct: 64 IVNDENREFAEELREKAKFVISYGTCAVYGGIPGLRNLWDKDEVIEEAYINSITTPNEEG 123
Query: 107 VYGDAKVKFQPKKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYP---V 163
V V + +P+ + I VD+ + GCPP A+ L G E+I+ P +
Sbjct: 124 VIPSEDVPHLEGRVKPLGEVIDVDFEVPGCPPRSDVAAEAVMALLTG---EEIELPETNL 180
Query: 164 CLECRLNGYP--------------------CVLLEKGEPCLGPITRAGCNARCPGFGIAC 203
C C P L+ +G C+GP T + C A P I C
Sbjct: 181 CEVCPREKPPEGLAMDFIKRQFEVGKPEDDLCLIPQGLICMGPATVSICGAEXPSIAIPC 240
Query: 204 IGCRG 208
GC G
Sbjct: 241 RGCYG 245
>sp|P22319|HOXY_ALCEU NAD-REDUCING HYDROGENASE HOXS DELTA SUBUNIT >gi|95478|pir||C35385
hydrogen dehydrogenase (EC 1.12.1.2) delta chain -
Alcaligenes eutrophus >gi|141949|gb|AAC06142.1| (M55230)
NAD-reducing hydrogenase [Ralstonia eutropha]
Length = 209
Score = 82.7 bits (201), Expect = 4e-15
Identities = 56/175 (32%), Positives = 88/175 (50%), Gaps = 19/175 (10%)
Query: 2 GKLRIGFYALTSCYGCQLQLAMMDE-LLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGS 60
GK+++ L C+GC L MDE LL L+ ++ + D + ++ I F+EG
Sbjct: 29 GKIKVATIGLCGCWGCTLSFLDMDERLLPLLEKVTLLRSSLTDIKRIPERCA-IGFVEGG 87
Query: 61 VSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDK-SLEELWKTVY---------GD 110
VS+EE +E ++ REN I+++VGACAV GGV + + L++ Y
Sbjct: 88 VSSEENIETLEHFRENCDILISVGACAVWGGVPAMRNVFELKDCLAEAYVNSATAVPGAK 147
Query: 111 AKVKFQP------KKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDI 159
A V F P K P + +K+DY I GCPP+ D ++ + L+ P D+
Sbjct: 148 AVVPFHPDIPRITTKVYPCHEVVKMDYFIPGCPPD-GDAIFKVLDDLVNGRPFDL 201
>pir||S32834 methylviologen-reducing hydrogenase chain vhcG [similarity] -
Methanococcus voltae >gi|296615|emb|CAA43505.1| (X61203)
methylviologen reducing hydrogenase subunit
[Methanococcus voltae]
Length = 306
Score = 81.9 bits (199), Expect = 7e-15
Identities = 63/232 (27%), Positives = 102/232 (43%), Gaps = 39/232 (16%)
Query: 14 CYGCQLQLA-MMDELLKLIPNAEIV-CWYMLDRDSVEDKPVDIAFIEGSVSTEEEVELVK 71
C GC + L + ++LL ++ E++ ++D + D +D+A IEG V + E + + +
Sbjct: 12 CSGCHISLIDLHEDLLDILEQVELIHSPVLMDVREIPDD-IDVALIEGGVRSGENLHIAR 70
Query: 72 KIRENAKIVVAVGACAVQGGVQSW-----TDKSLEELWKT---------VYGDAKVKFQP 117
++RE AK+V+A G CAV GG+ ++ LE+ +KT + +V
Sbjct: 71 EMREEAKVVIAFGTCAVYGGIPGMGNLYSNEELLEKAYKTTITTKNDEGIIPSEEVPALT 130
Query: 118 KKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLEC---------- 167
+ P+S+ I VDY PP+ L L PE + +C C
Sbjct: 131 SRVTPLSQVIDVDYIFQDAPPKPELIASVLTALLEEKEPELSNKNMCEVCPREKSSEGVV 190
Query: 168 ----------RLNGYPCVLLEKGEPCLGPITRAGCNARCPGF-GIACIGCRG 208
++ C LLE+G C+G TRA C C + C GC G
Sbjct: 191 VGELKRNYEGEIDPKKC-LLEQGYVCMGVATRASCRCFCALVRHVPCTGCYG 241
>pir||S16726 methylviologen-reducing hydrogenase chain vhcG - Methanococcus
voltae
Length = 289
Score = 80.8 bits (196), Expect = 2e-14
Identities = 62/220 (28%), Positives = 98/220 (44%), Gaps = 32/220 (14%)
Query: 14 CYGCQLQLA-MMDELLKLIPNAEIV-CWYMLDRDSVEDKPVDIAFIEGSVSTEEEVELVK 71
C GC + L + ++LL ++ E++ ++D + D +D+A IEG V + E + + +
Sbjct: 12 CSGCHISLIDLHEDLLDILEQVELIHSPVLMDVREIPDD-IDVALIEGGVRSGENLHIAR 70
Query: 72 KIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKT--VYGDAKVKFQPKKAEPVSKYIKV 129
++RE AK+V+A G CAV GG+ + L+ T + A V + P+S+ I V
Sbjct: 71 EMREEAKVVIAFGTCAVYGGI-----PGMGNLYSTAELLEKAPVLHLTSRVTPLSQVIDV 125
Query: 130 DYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLEC--------------------RL 169
DY PP+ L L PE + +C C +
Sbjct: 126 DYIFQDAPPKPELIASVLTALLEEKEPELSNKNMCEVCPREKSSEGVVVGELKRNYEGEI 185
Query: 170 NGYPCVLLEKGEPCLGPITRAGCNARCPGF-GIACIGCRG 208
+ C LLE+G C+G TRA C C + C GC G
Sbjct: 186 DPKKC-LLEQGYVCMGVATRASCRCFCALVRHVPCTGCYG 224
>gb|AAB57891.1| (U70364) NAD-reducing hydrogenase delta subunit [Rhodococcus
opacus]
Length = 209
Score = 79.6 bits (193), Expect = 4e-14
Identities = 56/178 (31%), Positives = 87/178 (48%), Gaps = 27/178 (15%)
Query: 3 KLRIGFYALTSCYGCQLQLAMMDELLKLIPNAEIVCWYMLDRDSVED-----KPVDIAFI 57
K+++ L C+GC L MDE L ++ + L R S+ D + I FI
Sbjct: 30 KIKVAMIGLCGCWGCTLSFLDMDERLLVLLDK-----VTLHRSSLSDIKRITERCAIGFI 84
Query: 58 EGSVSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTDK-SLEELWKTVYGDAK---- 112
EG V+ EE +E ++ REN ++++VGACAV GGV + + L++ VY D+
Sbjct: 85 EGGVANEENIETLEHYRENCDVLISVGACAVWGGVPAMRNVFELKDCLSEVYIDSATSVP 144
Query: 113 -----VKFQP------KKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDI 159
V F P K P + +K+DY I GCPP+ D ++ + L+ P D+
Sbjct: 145 GAKPVVPFHPDIPRITDKVYPCHEVVKMDYFIPGCPPD-ADAIFKVLDDLVNGRPFDL 201
>emb|CAA56463.1| (X80180) hydrogenase small beta subunit [Acetomicrobium flavidum]
Length = 179
Score = 76.1 bits (184), Expect = 4e-13
Identities = 51/172 (29%), Positives = 79/172 (45%), Gaps = 17/172 (9%)
Query: 1 MGKLRIGFYALTSCYGCQLQLAMMDE-LLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEG 59
M K ++ + L +C GC + +DE L+ L N EI+ ++D + + +D+ + G
Sbjct: 1 MAKAKVATFWLEACAGCHMSFLDLDERLIDLFQNVEILFSPIVDAKDIPN--IDVGVLSG 58
Query: 60 SVSTEEEVELVKKIRENAKIVVAVGACAVQGGVQSWTD---------KSLEELWKTVYGD 110
+ EEVEL KK+RE K +VA G CAV GG+ + + E TV
Sbjct: 59 GLGNVEEVELAKKMRERCKYLVAWGDCAVFGGINCMRNFIPKDVVLREGYIETASTVNPQ 118
Query: 111 AKVKFQP-----KKAEPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPE 157
V + +A P+ +KVD + GCPP+ Y L G P+
Sbjct: 119 GIVPSEDIPELLPRALPIDYEVKVDVYVPGCPPDADTIYYVFKELLAGRVPK 170
>sp|Q60340|FRHG_METJA COENZYME F420 HYDROGENASE GAMMA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE GAMMA
SUBUNIT) (FRH) >gi|2127831|pir||G64303 coenzyme F420
hydrogenase gamma chain homolog - Methanococcus
jannaschii >gi|1590833|gb|AAB98010.1| (U67461) coenzyme
F420-reducing hydrogenase, gamma subunit [Methanococcus
jannaschii]
Length = 230
Score = 74.1 bits (179), Expect = 2e-12
Identities = 65/208 (31%), Positives = 99/208 (47%), Gaps = 29/208 (13%)
Query: 4 LRIGFYALTSCYGCQLQLA-MMDELLKLIPNAEIVCWYMLDRDSVEDKPVDIAFIEGSVS 62
+++ L SC GC + LA ++LL ++ + E+V L D+ E DIA +EGSV
Sbjct: 5 VKVAHVQLCSCCGCLVSLADTYEKLLDVLNSIELVYCQTL-ADAREIPECDIALVEGSVC 63
Query: 63 TEEE--VELVKKIRENAKIVVAVGACAVQGGVQSWTDKSLEELWKTVYGDAKVKFQPKKA 120
++ +E+ +++R+ AKIVVA+GACA GGV + + +L K V+
Sbjct: 64 LDDHHSLEVAQEVRKKAKIVVALGACAATGGVTRYCKGN--QLSKPVH---------SSF 112
Query: 121 EPVSKYIKVDYNIYGCPPEKRDFLYALGTFLIGSWPEDIDYPVCLECRLNGYPCVLLEKG 180
P+++ IKVD I GCPP + + L G D++Y L Y L EKG
Sbjct: 113 SPLTEVIKVDLAIPGCPPSPEAIVGVITAALNG----DMEY-------LQPY-AELAEKG 160
Query: 181 EPCLG--PITRAGCNARCPGFGIACIGC 206
G I + + C G G C
Sbjct: 161 SEACGCDVIYKVVNKSLCMGCGTCAAAC 188
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.322 0.141 0.444
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 102138798
Number of Sequences: 2977
Number of extensions: 4529452
Number of successful extensions: 12935
Number of sequences better than 1.0e-10: 20
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 12887
Number of HSP's gapped (non-prelim): 20
length of query: 261
length of database: 189,106,746
effective HSP length: 50
effective length of query: 211
effective length of database: 159,182,396
effective search space: 33587485556
effective search space used: 33587485556
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 164 (68.3 bits)