BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1787 (hydA-1) DE:CYTOCHROME-C3 HYDROGENASE ALPHA CHAIN
(428 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75133 cytochrome-c3 hydrogenase alpha chain PAB1787 - Pyro... 859 0.0
pir||S48836 cytochrome-c3 hydrogenase (EC 1.12.2.1) alpha chain ... 793 0.0
pir||C71075 probable cytochrome-c3 hydrogenase alpha chain - Pyr... 769 0.0
gb|AAB94936.1| (AF039208) NiFe hydrogenase alpha subunit [Thermo... 691 0.0
pir||B75070 cytochrome-c3 hydrogenase alpha chain PAB0641 - Pyro... 298 1e-79
gb|AAF61854.1|AF176650_4 (AF176650) sulfhydrogenase II subunit a... 293 3e-78
pir||B37777 methyl viologen-reducing hydrogenase large chain - M... 170 2e-41
pir||E30315 methyl viologen-reducing hydrogenase alpha chain - M... 167 3e-40
emb|CAA56464.1| (X80180) hydrogenase large alpha subunit [Acetom... 166 4e-40
pir||A69018 probable coenzyme F420 hydrogenase (EC 1.12.99.1) al... 166 4e-40
pir||G64448 methylviologen-reducing hydrogenase alpha chain homo... 163 4e-39
pir||S24801 methylviologen-reducing hydrogenase chain vhuA - Met... 158 1e-37
pir||A59304 methylviologen-reducing hydrogenase alpha chain vhuA... 157 2e-37
gi|11498968 methylviologen-reducing hydrogenase, subunit alpha (... 146 6e-34
pir||S16727 coenzyme F420 hydrogenase (EC 1.12.99.1) alpha chain... 135 8e-31
pir||S75530 hydrogenase large chain - Synechocystis sp. (strain ... 134 2e-30
pir||S74233 bidirectional hydrogenase chain H - Synechococcus sp... 133 4e-30
pir||S72553 hydrogenase large chain - Anabaena variabilis >gi|21... 130 4e-29
sp|Q60338|FRHA_METJA COENZYME F420 HYDROGENASE ALPHA SUBUNIT (8-... 130 4e-29
sp|Q00390|FRHA_METVO COENZYME F420 HYDROGENASE ALPHA SUBUNIT (8-... 128 1e-28
gb|AAB53705.1| (U88400) bidirectional hydrogenase large subunit ... 121 2e-26
sp|P22320|HOXH_ALCEU NAD-REDUCING HYDROGENASE HOXS BETA SUBUNIT ... 119 5e-26
sp|P19496|FRHA_METTH COENZYME F420 HYDROGENASE ALPHA SUBUNIT (8-... 119 7e-26
gb|AAB85780.1| (AE000895) coenzyme F420-reducing hydrogenase, al... 119 7e-26
emb|CAA43500.1| (X61202) F420-reducing hydrogenase subunit [Meth... 116 3e-25
gb|AAB57892.1| (U70364) NAD-reducing hydrogenase beta subunit [R... 114 2e-24
emb|CAA74094.1| (Y13764) F420-reducing hydrogenase isoenzyme I a... 113 5e-24
emb|CAA74090.1| (Y13763) F420-reducing hydrogenase isoenzyme II ... 110 3e-23
pir||A70370 hydrogenase large subunit - Aquifex aeolicus >gi|298... 109 8e-23
sp|P13065|PHSL_DESBA PERIPLASMIC [NIFESE] HYDROGENASE LARGE SUBU... 107 3e-22
pir||HQDVLB cytochrome-c3 hydrogenase (EC 1.12.2.1) (NiFeSe) lar... 105 9e-22
pdb|1CC1|L Chain L, Crystal Structure Of A Reduced, Active Form ... 99 1e-19
gb|AAA23375.2| (M18271) large hydrogenase subunit [Desulfomicrob... 96 1e-18
pir||F30315 methyl viologen-reducing hydrogenase (EC 1.-.-.-) al... 95 1e-18
gb|AAB49361.1| (U82565) [NiFe] hydrogenase-like protein large su... 69 9e-11
>pir||B75133 cytochrome-c3 hydrogenase alpha chain PAB1787 - Pyrococcus abyssi
(strain Orsay) >gi|5458290|emb|CAB49779.1| (AJ248285)
CYTOCHROME-C3 HYDROGENASE ALPHA CHAIN [Pyrococcus
abyssi]
Length = 428
Score = 859 bits (2195), Expect = 0.0
Identities = 428/428 (100%), Positives = 428/428 (100%)
Query: 1 MRNLYIPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAI 60
MRNLYIPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAI
Sbjct: 1 MRNLYIPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAI 60
Query: 61 YPRICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY 120
YPRICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY
Sbjct: 61 YPRICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY 120
Query: 121 LGYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEE 180
LGYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEE
Sbjct: 121 LGYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEE 180
Query: 181 MKAKLRESLSLAEYTFELFAKLEQYREVEGEITHLAVKPRGDVYGIYGDYIKASDGEEFP 240
MKAKLRESLSLAEYTFELFAKLEQYREVEGEITHLAVKPRGDVYGIYGDYIKASDGEEFP
Sbjct: 181 MKAKLRESLSLAEYTFELFAKLEQYREVEGEITHLAVKPRGDVYGIYGDYIKASDGEEFP 240
Query: 241 SEDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKG 300
SEDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKG
Sbjct: 241 SEDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKG 300
Query: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVY 360
TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVY
Sbjct: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVY 360
Query: 361 ALKVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCIS 420
ALKVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCIS
Sbjct: 361 ALKVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCIS 420
Query: 421 CSVHVVKL 428
CSVHVVKL
Sbjct: 421 CSVHVVKL 428
>pir||S48836 cytochrome-c3 hydrogenase (EC 1.12.2.1) alpha chain - Pyrococcus
furiosus >gi|562777|emb|CAA53037.1| (X75255) hydrogenase
(alpha subunit) [Pyrococcus furiosus]
Length = 428
Score = 793 bits (2026), Expect = 0.0
Identities = 380/428 (88%), Positives = 413/428 (95%)
Query: 1 MRNLYIPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAI 60
M+NLY+PIT+DHIARVEGKGGVEII+GD+GVKEVKLNIIEGPRFFEAITIGKKLEEALAI
Sbjct: 1 MKNLYLPITIDHIARVEGKGGVEIIIGDDGVKEVKLNIIEGPRFFEAITIGKKLEEALAI 60
Query: 61 YPRICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY 120
YPRICSFCSAAHKLTALEAAEKA+GF PREEIQALREVLYIGDMIESHALHLYLLVLPDY
Sbjct: 61 YPRICSFCSAAHKLTALEAAEKAVGFVPREEIQALREVLYIGDMIESHALHLYLLVLPDY 120
Query: 121 LGYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEE 180
GYSSPLKMVNEYK+E+EIALKLKNLG+WMMD+LGSRAIHQENA+LGGFGKLP K LE+
Sbjct: 121 RGYSSPLKMVNEYKREIEIALKLKNLGTWMMDILGSRAIHQENAVLGGFGKLPEKSVLEK 180
Query: 181 MKAKLRESLSLAEYTFELFAKLEQYREVEGEITHLAVKPRGDVYGIYGDYIKASDGEEFP 240
MKA+LRE+L LAEYTFELFAKLEQY EVEG ITHLAVKPRGD YGIYGDYIKASDGEEFP
Sbjct: 181 MKAELREALPLAEYTFELFAKLEQYSEVEGPITHLAVKPRGDAYGIYGDYIKASDGEEFP 240
Query: 241 SEDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKG 300
SE Y+++I EFVVEHSFAKHSHYKG+PFMVGAISRV+NN DLLYG+AK+LYE++K+LLKG
Sbjct: 241 SEKYRDYIKEFVVEHSFAKHSHYKGRPFMVGAISRVINNADLLYGKAKELYEANKDLLKG 300
Query: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVY 360
TNPFANNLAQALE+VYFIERAIDL+DE L KWP+K RD+VE++DGFGVSTTEAPRGILVY
Sbjct: 301 TNPFANNLAQALEIVYFIERAIDLLDEALAKWPIKPRDEVEIKDGFGVSTTEAPRGILVY 360
Query: 361 ALKVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCIS 420
ALKVENGRV+YADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLK+LAEMVVRAYDPCIS
Sbjct: 361 ALKVENGRVSYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKILAEMVVRAYDPCIS 420
Query: 421 CSVHVVKL 428
CSVHVV+L
Sbjct: 421 CSVHVVRL 428
>pir||C71075 probable cytochrome-c3 hydrogenase alpha chain - Pyrococcus
horikoshii >gi|3257714|dbj|BAA30397.1| (AP000005) 429aa
long hypothetical cytochrome-c3 hydrogenase alpha chain
[Pyrococcus horikoshii]
Length = 429
Score = 769 bits (1964), Expect = 0.0
Identities = 372/428 (86%), Positives = 410/428 (94%)
Query: 1 MRNLYIPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAI 60
M+ +YIPITVDHIAR+EGK GVEI+VG++GVKEVKLNIIEGPRFFEAIT+GKKLEEALAI
Sbjct: 1 MKEIYIPITVDHIARIEGKAGVEILVGEDGVKEVKLNIIEGPRFFEAITLGKKLEEALAI 60
Query: 61 YPRICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY 120
YPRICSFCSAAHKLTALEAAEKAIGFTPREEIQALRE+LYIGD+IESHALHLYLLVLPDY
Sbjct: 61 YPRICSFCSAAHKLTALEAAEKAIGFTPREEIQALREILYIGDIIESHALHLYLLVLPDY 120
Query: 121 LGYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEE 180
LGYSSPLKMV+EYKKELE A+KLKNLGSW+MDVLG+RAIHQENAILGGFGKLPSKETLE+
Sbjct: 121 LGYSSPLKMVDEYKKELETAIKLKNLGSWIMDVLGARAIHQENAILGGFGKLPSKETLEK 180
Query: 181 MKAKLRESLSLAEYTFELFAKLEQYREVEGEITHLAVKPRGDVYGIYGDYIKASDGEEFP 240
+K +L+ +L LAEYTFELF+KLEQY+EVEGEITHLAVKPR D YGIYGD IKASDGEEFP
Sbjct: 181 IKDELKSALPLAEYTFELFSKLEQYKEVEGEITHLAVKPRKDAYGIYGDRIKASDGEEFP 240
Query: 241 SEDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKG 300
SE+YK +I EFVVEHSFAKHSHYKG+PFMVGAISR+VNN LLYG+AK+LYE++K+LL+
Sbjct: 241 SEEYKNYIKEFVVEHSFAKHSHYKGRPFMVGAISRLVNNHKLLYGKAKELYENNKDLLRP 300
Query: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVY 360
TNPFANNLAQALE+VYF+ERAIDLIDEVL KWP+K RD+V+VRDGFGVSTTEAPRGILVY
Sbjct: 301 TNPFANNLAQALEIVYFMERAIDLIDEVLAKWPIKPRDEVKVRDGFGVSTTEAPRGILVY 360
Query: 361 ALKVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCIS 420
ALKVENGRV+YADIITPTAFNLAMME HVRMMAE+HY DDPE+LKLLAEMVVRAYDPCIS
Sbjct: 361 ALKVENGRVSYADIITPTAFNLAMMERHVRMMAEEHYKDDPEKLKLLAEMVVRAYDPCIS 420
Query: 421 CSVHVVKL 428
CSVHVVKL
Sbjct: 421 CSVHVVKL 428
>gb|AAB94936.1| (AF039208) NiFe hydrogenase alpha subunit [Thermococcus litoralis]
Length = 426
Score = 691 bits (1765), Expect = 0.0
Identities = 335/426 (78%), Positives = 382/426 (89%), Gaps = 1/426 (0%)
Query: 4 LYIPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPR 63
+YIPITVDHIARVEGKGG+EI+ DEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPR
Sbjct: 1 MYIPITVDHIARVEGKGGIEIVTSDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPR 60
Query: 64 ICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGY 123
+CSFCSA+HKLTALEAAEKAIGFTPR EIQ LRE+LY+GD IESHALHLYLLVLPDYLGY
Sbjct: 61 VCSFCSASHKLTALEAAEKAIGFTPRPEIQDLRELLYMGDTIESHALHLYLLVLPDYLGY 120
Query: 124 SSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKA 183
S+PL MV++YKKE+E A+ LKN+GS +MD LGSRAIHQEN +LGGFGKLP+K EE++
Sbjct: 121 SNPLAMVDKYKKEIEYAMALKNVGSKIMDYLGSRAIHQENVVLGGFGKLPTKAQFEELRR 180
Query: 184 KLRESLSLAEYTFELFAKLEQYREV-EGEITHLAVKPRGDVYGIYGDYIKASDGEEFPSE 242
+L+E+L LAEYT ELF+KLEQY EV + E+ H+AVKPR DVYGIYGDYIK SDG EFP E
Sbjct: 181 ELKEALPLAEYTVELFSKLEQYEEVTDDEMVHMAVKPRNDVYGIYGDYIKVSDGFEFPVE 240
Query: 243 DYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGTN 302
DYK+HI E VVEHSFAKHS YKGKPFMVGAISR+VNN DLLYG+AK+LY +K+LL+ N
Sbjct: 241 DYKKHIVEKVVEHSFAKHSFYKGKPFMVGAISRIVNNADLLYGKAKELYTQYKDLLRYNN 300
Query: 303 PFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVYAL 362
FANN AQA+ELVYFIE++ID+ID+ L KWPVKERD+VE++DGFGVS TEAPRG+LVYAL
Sbjct: 301 CFANNFAQAIELVYFIEKSIDIIDDTLAKWPVKERDEVELKDGFGVSITEAPRGLLVYAL 360
Query: 363 KVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCISCS 422
+V++GRV YADIITPTA NLA+ME HVRMMAE H+ DDPERLKLLAEM VRAYDPCISCS
Sbjct: 361 EVKDGRVNYADIITPTAMNLAIMERHVRMMAENHWQDDPERLKLLAEMTVRAYDPCISCS 420
Query: 423 VHVVKL 428
VHVV+L
Sbjct: 421 VHVVRL 426
>pir||B75070 cytochrome-c3 hydrogenase alpha chain PAB0641 - Pyrococcus abyssi
(strain Orsay) >gi|5458375|emb|CAB49863.1| (AJ248286)
CYTOCHROME-C3 HYDROGENASE ALPHA CHAIN [Pyrococcus
abyssi]
Length = 415
Score = 298 bits (754), Expect = 1e-79
Identities = 166/425 (39%), Positives = 246/425 (57%), Gaps = 19/425 (4%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICSF 67
I +D RVEG G EI++ + VK+ ++ I+EGPRFFE +T+G+ + + RIC+
Sbjct: 6 IELDEFTRVEGIGKAEIVIENGTVKDARVKILEGPRFFEVLTLGRHYMDVPDLEARICAI 65
Query: 68 CSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSPL 127
C A+H + ++ A E A+G E ++ LRE+ G++IESHALHLYLL LPD GY +
Sbjct: 66 CYASHSIASVRAIENALGIEVSESVEKLRELALWGEIIESHALHLYLLALPDVYGYPDAI 125
Query: 128 KMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKLRE 187
MV++Y + ++ L +K LG+ + + + R IH N GGFG+ PS+ LE++ R
Sbjct: 126 SMVSKYGELIKEGLTIKALGNMIRETIAGREIHGINVKPGGFGRYPSERELEKIAKHARA 185
Query: 188 SLSLAEYTFELFAKLEQYREVEGEITHLAVKPRG---DVYGIYGDYIKASDGEEFPSEDY 244
+ A +FA E PRG +V + +Y+ +
Sbjct: 186 LVRFARRIVNIFATQE---------------PRGAKIEVPMVTSEYLWGDELIAGNDRIQ 230
Query: 245 KEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGTNPF 304
E I E V +SFAKHS+YKGKP VGA++R++ L G AK L +S+KE +K +
Sbjct: 231 YEDIEEVPVPYSFAKHSYYKGKPIFVGALARLLLKGSKLEGEAKRLLDSYKEKIKSGHVI 290
Query: 305 ANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVYALKV 364
NNLAQA+ELVY +E A + +E++ + K + + G G+ EAPRG+LV+ ++
Sbjct: 291 YNNLAQAIELVYALEVASKIAEELISEGIEKSTCEPSEKSGEGIGYVEAPRGVLVHHYRI 350
Query: 365 ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPE-RLKLLAEMVVRAYDPCISCSV 423
EN R+ +++ ITPTAFN MME+ + A + + E LK E VVRA+DPCISCSV
Sbjct: 351 ENERIVWSNTITPTAFNQGMMEKSLLEDARNMFGKESETTLKSKLEEVVRAFDPCISCSV 410
Query: 424 HVVKL 428
HVV+L
Sbjct: 411 HVVRL 415
>gb|AAF61854.1|AF176650_4 (AF176650) sulfhydrogenase II subunit a [Pyrococcus furiosus]
Length = 412
Score = 293 bits (742), Expect = 3e-78
Identities = 158/422 (37%), Positives = 247/422 (58%), Gaps = 13/422 (3%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICSF 67
I +D RVEG G EI++ + VK+ ++ I+EGPRFFE +T+G+ + + RIC+
Sbjct: 3 IELDEFTRVEGNGKAEIVIENGEVKDARVKIVEGPRFFEILTLGRDYWDVPDLEARICAI 62
Query: 68 CSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSPL 127
C AH + ++ A EKA+G E ++ LRE+ G++IESHALHLYLL LPD GY +
Sbjct: 63 CYIAHSVASVRAIEKALGIDVPESVEKLRELALWGEIIESHALHLYLLALPDVFGYPDAI 122
Query: 128 KMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKLRE 187
M+ + + ++ L +K G+ + +++G R IH N GGFG+ PS+E LE++ +
Sbjct: 123 SMIPRHGELVKEGLTIKAFGNAIRELIGGREIHGINIKPGGFGRYPSEEELEKIAEHSKS 182
Query: 188 SLSLAEYTFELFAKLEQYREVEGEITHLAVKPRGDVYGIYGDYIKASDGEEFPSEDYKEH 247
+ A +FA E V + + GD I G+ ++ + +E P
Sbjct: 183 LIKFARRIVGIFASQEAGGAVGEVLMATSDYLWGDELIINGERVQYYEVDEVP------- 235
Query: 248 INEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGTNPFANN 307
V +SFAKHS+YKG P VGA+ R++ + + G A + E +++ L+ NN
Sbjct: 236 -----VGYSFAKHSYYKGNPVFVGALPRLLLKGESIEGEAARMLEEYRDKLESKYVIYNN 290
Query: 308 LAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVYALKVENG 367
LAQA+EL+Y +ER L++E+L + + ++ G GV EAPRG+LV+ ++ENG
Sbjct: 291 LAQAIELLYALERVPQLVEEILSEGIERGNGEISQESGEGVGYVEAPRGVLVHHYRIENG 350
Query: 368 RVAYADIITPTAFNLAMMEEHVRMMAEKHY-NDDPERLKLLAEMVVRAYDPCISCSVHVV 426
+V +++ ITPTAFN +ME + A++ Y ++ E +K E++VRA+DPCISCSVH V
Sbjct: 351 KVVWSNTITPTAFNQRLMELSLLEEAKRLYGSESEENMKKRLEVIVRAFDPCISCSVHFV 410
Query: 427 KL 428
KL
Sbjct: 411 KL 412
>pir||B37777 methyl viologen-reducing hydrogenase large chain - Methanothermus
fervidus >gi|149805|gb|AAA72832.1| (M34016) methyl
viologen-reducing hydrogenase large subunit (mvhA)
[Methanothermus fervidus]
Length = 472
Score = 170 bits (427), Expect = 2e-41
Identities = 131/452 (28%), Positives = 221/452 (47%), Gaps = 38/452 (8%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
+ ++ + R+EG + + + +EG VK+ + +++E R FE G+++EEA I PRIC
Sbjct: 4 LVLEPVTRIEGHAKITVQLDEEGNVKDTRFHVMEF-RGFEKFLQGRRIEEAPRIVPRICG 62
Query: 67 FCSAAHKLTALEAAEKAIGFTPR---EEIQALREVLYIGDMIESHALHLYLLVLPDYLG- 122
C H L + +A + GF P E +RE++ + SH LH Y L PD++G
Sbjct: 63 ICQVQHHLASAKAVDACFGFEPEDIPETAYKMREIMNWASYVHSHGLHFYFLAAPDFIGG 122
Query: 123 ----YSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETL 178
+ K++ + + A++L+ ++ G RAIH + I GG KET
Sbjct: 123 KDRETRNIFKIIQDSPDVAKQAIELRKNAQDIVAATGGRAIHPVSFIPGGITTELDKETQ 182
Query: 179 EEMKAKLRESLSLAEYTFELFAKL--EQYREVEG----EITHLAVKPRGDVYGIYGDYIK 232
E++ K + ++ +AE T EL + + VE E H+ + G + +Y ++
Sbjct: 183 EKLLKKAKRNVEIAESTLELAIPIFEDNMDLVESLGTIETYHMGLVKNG-TWDVYDGVVR 241
Query: 233 ASD--GE---EFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKDL 282
D GE EF ++Y ++I E V +S+ K + K G P + V +SR +N D
Sbjct: 242 VKDKNGENFAEFGPDEYTKYIAEHVKPYSWLKFPYLKELGYPDGVYRVSPLSR-LNVADK 300
Query: 283 LYGRAKDLYESHKELLK----GTNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERD 338
+ A E KE P + A+ +E++ E +++E L K
Sbjct: 301 MPDAAPKAQEYFKEFRNKFGYAQQPLLYHWARLIEMLAAAECMASVLEEDLS--GKKIHG 358
Query: 339 KVEVRDGFGVSTTEAPRGILVYALKVE-NGRVAYADIITPTAFNLAMMEEHVRMMAEKHY 397
K+E ++G GV EAPRG L++ + NG + A++I T N ME ++ +A++
Sbjct: 359 KLERQEGEGVGIVEAPRGTLIHHYACDKNGIITKANLIVATVQNNPAMEMGIQKVAKERI 418
Query: 398 N---DDPERLKLLAEMVVRAYDPCISCSVHVV 426
D +++ L EMV+RAYDPC+SC+ H V
Sbjct: 419 KPGVDVDDKIYNLMEMVIRAYDPCLSCATHTV 450
>pir||E30315 methyl viologen-reducing hydrogenase alpha chain - Methanobacterium
thermoautotrophicum (strain delta H)
>gi|149733|gb|AAB02351.1| (J04540) mvhA gene product
[Methanobacterium thermoautotrophicum]
Length = 472
Score = 167 bits (418), Expect = 3e-40
Identities = 135/455 (29%), Positives = 222/455 (48%), Gaps = 40/455 (8%)
Query: 6 IPITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRI 64
+ +T++ + R+EG + + + D G V++ +L+++E R FE G+ +EEA I PRI
Sbjct: 2 VKLTMEPVTRIEGHAKITVRLDDAGNVEDTRLHVMEF-RGFEKFLQGRPIEEAPRIVPRI 60
Query: 65 CSFCSAAHKLTALEAAEKAIGFTPREEIQA---LREVLYIGDMIESHALHLYLLVLPDYL 121
C C H L A +A + GF P + + A +RE++ G + SH LH Y L PD++
Sbjct: 61 CGICDVQHHLAAAKAVDACFGFEPEDVLPAAYKMREIMNWGSYMHSHGLHFYFLAAPDFI 120
Query: 122 -GYSSPLKMVNEYKKEL-EIALKLKNLGSWMMDVL---GSRAIHQENAILGGFGKLPSKE 176
G + V + K+ +IAL+ L ++++ G R IH ++ GG E
Sbjct: 121 AGKDRKTRNVFQIIKDAPDIALQAIELRKNALELVRATGGRPIHPTSSTPGGISTELDDE 180
Query: 177 TLEEMKAKLRESLSLAEYTFELFAKL-EQYREVEGEITHLAVKPRG----DVYGIYGDYI 231
T +++ K + ++ LAE T EL + E+ ++ + ++ G V+ +Y +
Sbjct: 181 TQKDLLKKAQRNVELAEATLELAVPIFEENIDLVNSLGNIETYHTGLVKDGVWDVYDGIV 240
Query: 232 KASDGE-----EFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKD 281
+ D E EF DY + I E V +S+ K + K G P + V +SR +N D
Sbjct: 241 RIKDKEGNMFREFKPADYADTIAEHVTPYSWLKFPYIKDLGYPDGVYRVSPLSR-LNVAD 299
Query: 282 LLYGRAKDLYESHKELLKGTNPFANNL----AQALELVYFIERAIDLIDEVLI--KWPVK 335
+ A E KE + L A+ +EL+ E A D ++ L K+P
Sbjct: 300 KMPDAAPKAQEHFKEFRENFGYAQQTLLYHWARLIELLACAECAADALEGDLSGEKFP-- 357
Query: 336 ERDKVEVRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAE 394
D +E + G GV EAPRG L + ENG + A+I+ T N ME ++ +A+
Sbjct: 358 --DSLERQAGDGVGIVEAPRGTLTHHYTCDENGLITKANIVVATIQNNPAMEMGIQKVAQ 415
Query: 395 KHYNDDPE---RLKLLAEMVVRAYDPCISCSVHVV 426
+ E ++ L EMV+RAYDPC+SC+ H +
Sbjct: 416 DYIKPGVEVDDKIFNLMEMVIRAYDPCLSCATHTI 450
>emb|CAA56464.1| (X80180) hydrogenase large alpha subunit [Acetomicrobium flavidum]
Length = 475
Score = 166 bits (416), Expect = 4e-40
Identities = 132/454 (29%), Positives = 228/454 (50%), Gaps = 43/454 (9%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
+ ++ + R+EG G + +++ + G V+E + ++ + R FE T G+ E I PRIC
Sbjct: 7 LEINPVTRIEGHGKITVMLDESGHVRETRFHVTQY-RGFEVFTHGRDFREMPVITPRICG 65
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPD-YLGYSS 125
C +H L + +A ++ +G T LRE++++G +++SHAL + L PD LG+ +
Sbjct: 66 ICPVSHHLASAKACDEILGVTITPAAHKLRELMHMGQIVQSHALSFFHLSSPDILLGFDA 125
Query: 126 PLK------MVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLE 179
P+K +V+ Y + + + L+ G ++ LG + IH ++I GG + + + +
Sbjct: 126 PVKIRNVAGLVDRYPELAKKGIMLRKFGQEIIKTLGGKKIHPWHSIPGGVNRSLTPQERD 185
Query: 180 EMKAKLRESLSLAEYTFELFAKLEQYREVEGEITHLAVKPRGDV--YGIYGD-YIKASDG 236
+ A+L E S+A E ++ Y + GE L P D G+ D Y++ DG
Sbjct: 186 AIAAQLPEMKSIA---MEAIKLIKDYLQEGGE--ELKEFPTLDTAYMGLVRDGYLELYDG 240
Query: 237 E------------EFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNN 279
E +F +DY +HI E V S+ K YK G P + VG ++R+
Sbjct: 241 EVRIKAPRGRILDQFDPKDYLDHIGEHVEPWSYLKFPFYKALGFPHGSYRVGPLARLNAA 300
Query: 280 KDLLYGRAKDLYESHKELLK-GTNPFA--NNLAQALELVYFIERAIDLI-DEVLIKWPVK 335
+ A + +KE+ + G P+ + A+ +E +Y +ER L+ D + ++
Sbjct: 301 DAVSTPEASKEFALYKEMGEDGIVPYTLYYHYARLIEALYGLERIEQLLADPDITSSDLR 360
Query: 336 ERDKVEVRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAE 394
K +G GV EAPRG L++ +V E+G + ++I T N M + V M+A+
Sbjct: 361 VTSKEINPEGIGV--IEAPRGTLIHHYQVNESGVITKVNLIVATGHNNFAMNKGVEMVAK 418
Query: 395 KHY--NDDPERLKLLAEMVVRAYDPCISCSVHVV 426
K+ + PE + E V+RAYDPC+SCS H V
Sbjct: 419 KYITGTNVPEGVFNRLEHVIRAYDPCLSCSTHAV 452
>pir||A69018 probable coenzyme F420 hydrogenase (EC 1.12.99.1) alpha chain -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2622238|gb|AAB85623.1| (AE000883) methyl
viologen-reducing hydrogenase, alpha subunit
[Methanobacterium thermoautotrophicum]
Length = 472
Score = 166 bits (416), Expect = 4e-40
Identities = 135/455 (29%), Positives = 222/455 (48%), Gaps = 40/455 (8%)
Query: 6 IPITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRI 64
+ +T++ + R+EG + + + D G V++ +L+++E R FE G+ +EEA I PRI
Sbjct: 2 VKLTMEPVTRIEGHAKITVHLDDAGNVEDTRLHVMEF-RGFEKFLQGRPIEEAPRIVPRI 60
Query: 65 CSFCSAAHKLTALEAAEKAIGFTPREEIQA---LREVLYIGDMIESHALHLYLLVLPDYL 121
C C H L A +A + GF P + + A +RE++ G + SH LH Y L PD++
Sbjct: 61 CGICDVQHHLAAAKAVDACFGFEPEDVLPAAYKMREIMNWGSYMHSHGLHFYFLAAPDFI 120
Query: 122 -GYSSPLKMVNEYKKEL-EIALKLKNLGSWMMDVL---GSRAIHQENAILGGFGKLPSKE 176
G + V + K+ +IAL+ L ++++ G R IH ++ GG E
Sbjct: 121 AGKDRKTRNVFQIIKDAPDIALQAIELRKNALELVRATGGRPIHPTSSTPGGISTELDDE 180
Query: 177 TLEEMKAKLRESLSLAEYTFELFAKL-EQYREVEGEITHLAVKPRG----DVYGIYGDYI 231
T +++ K + ++ LAE T EL + E+ ++ + ++ G V+ +Y +
Sbjct: 181 TQKDLLKKAQRNVELAEATLELAVPIFEENIDLVNSLGNIETYHTGLVKDGVWDVYDGIV 240
Query: 232 KASDGE-----EFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKD 281
+ D E EF DY + I E V +S+ K + K G P + V +SR +N D
Sbjct: 241 RIKDKEGNMFREFKPADYADTIAEHVKPYSWLKFPYIKDLGYPDGVYRVSPLSR-LNVAD 299
Query: 282 LLYGRAKDLYESHKELLKGTNPFANNL----AQALELVYFIERAIDLIDEVLI--KWPVK 335
+ A E KE + L A+ +EL+ E A D ++ L K+P
Sbjct: 300 KMPDAAPKAQEHFKEFRENFGYAQQTLLYHWARLIELLACAECAADALEGDLSGEKFP-- 357
Query: 336 ERDKVEVRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAE 394
D +E + G GV EAPRG L + ENG + A+I+ T N ME ++ +A+
Sbjct: 358 --DSLERQAGDGVGIVEAPRGTLTHHYTCDENGLITKANIVVATIQNNPAMEMGIQKVAQ 415
Query: 395 KHYNDDPE---RLKLLAEMVVRAYDPCISCSVHVV 426
+ E ++ L EMV+RAYDPC+SC+ H +
Sbjct: 416 DYIKPGVEVDDKIFNLMEMVIRAYDPCLSCATHTI 450
>pir||G64448 methylviologen-reducing hydrogenase alpha chain homolog -
Methanococcus jannaschii >gi|1591820|gb|AAB99194.1|
(U67560) methylviologen-reducing hydrogenase, alpha
chain (vhuA) [Methanococcus jannaschii]
Length = 418
Score = 163 bits (408), Expect = 4e-39
Identities = 124/413 (30%), Positives = 212/413 (51%), Gaps = 28/413 (6%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGV-KEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
I ++ ++R+EG G V I + + G K+VKL+I R FE +G+ EE I PRIC
Sbjct: 4 IVIEPLSRLEGHGKVTITLDENGKPKDVKLHIT-ALRGFEQFVVGRPAEEVPRIVPRICG 62
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYL----- 121
C AH L +++A + A G E + LRE+++IG+MI SHALH Y L PD++
Sbjct: 63 ICQTAHHLASVKAIDAAWGVEIPEPAKKLRELMHIGNMIHSHALHFYFLAAPDFVLGPDA 122
Query: 122 --GYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLE 179
+ + ++++ + A+ L+ G +++ +G +AIH I GG K ++E +
Sbjct: 123 DPAIRNIVGVIDKAPDVAKQAIALRKFGQKIVEAVGGKAIHPVTGIPGGQAKRLTEEERD 182
Query: 180 EMKAKLRESLSLAEYTFELFAKL-EQYREVEGEI----THLAVKPRGDVYGIYGDYIK-- 232
E+ + + A+ EL +L EQY E + T+ + + Y D ++
Sbjct: 183 ELLKDADQMIEYAKNGVELIKQLNEQYMEQIKTLGVIDTYYLGLVKDGKHNFYDDTLRFL 242
Query: 233 ASDGE---EFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKDLLY 284
+ DG+ EF E+Y +I E+VV +++ KH +YK G P + VG ++ + ++
Sbjct: 243 SPDGKEKVEFKPEEYLNYIGEYVVPYNYVKHPYYKKVGYPEGVYRVGPLAMLNVCDEMET 302
Query: 285 GRAKDLYESHKEL--LKGTNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEV 342
A++ + E+ A N A+ +ELV E+A L+++ I + D VE
Sbjct: 303 PLAEEYRKEFLEIFGFPANQSLAYNHARLIELVEACEKAKILLEDNDITSDDIKAD-VEP 361
Query: 343 RDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAE 394
+ G GV APRG+L++ + ENG V A++I T N+ ME+ ++ A+
Sbjct: 362 KAGNGVGVVYAPRGVLIHNYETDENGIVVKANMIVATTHNVPTMEKAIQQAAQ 414
>pir||S24801 methylviologen-reducing hydrogenase chain vhuA - Methanococcus
voltae
Length = 420
Score = 158 bits (396), Expect = 1e-37
Identities = 122/420 (29%), Positives = 206/420 (49%), Gaps = 30/420 (7%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVK-EVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
IT+ + R+EG G V I + D G +VKL+I R FE IG+ EE I PRIC
Sbjct: 4 ITIAPLTRLEGHGKVTIKLDDSGKPADVKLHIT-ALRGFEQFVIGRPAEEVPRIVPRICG 62
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYL----- 121
C AH L +++A + A G + RE++++G+MI SHALH Y L PD++
Sbjct: 63 ICQTAHHLASVKAVDAAWGAQIPSAAEKQRELMHLGNMIHSHALHFYYLAAPDFVLGPDA 122
Query: 122 --GYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLE 179
+ + +++ + + A+ ++ +G M++ G + IH I GG K S+E +
Sbjct: 123 DPAIRNIVGVIDARPEVRKKAIAMRRVGQSMVEATGGKPIHPVTGIPGGLSKSMSEEKRD 182
Query: 180 EMKAKLRESLSLAEYTFELFAKL-EQYREVEGEITHLAVKPRGDV----YGIYGDYIK-- 232
E+ A++ + + +L L E+Y + + + G V + YGD ++
Sbjct: 183 ELLAEIDTMIQYGQDGLDLMKSLNEKYLDTINSLGVIDTWYLGFVKDGKHNFYGDTLRFV 242
Query: 233 ---ASDGEEFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKDLLY 284
S+ EF +Y +++ E VVEHS+ K+ + K G P + VG ++ + +
Sbjct: 243 SPDGSEKMEFKPAEYLDYLGEHVVEHSYVKYPYNKKEGYPEGLYRVGPLAMINVCDSMST 302
Query: 285 GRAKDLYESHKELL--KGTNPFANNLAQALELVYFIERAIDLI-DEVLIKWPVKERDKVE 341
A++ + E A N A+ +EL+ ERA +L+ D ++ VK +VE
Sbjct: 303 PLAEEARKEFAETFGRPANQSIAYNQARLIELLSACERAKELLEDPEIVSTDVKA--EVE 360
Query: 342 VRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDD 400
+ G GV APRG L + + +NG V A++I T N+ ME+ ++ AE + D+
Sbjct: 361 PKAGIGVGVVYAPRGTLFHNYETDDNGIVTKANMIVATTHNVPTMEKAIQQAAEVLFKDN 420
>pir||A59304 methylviologen-reducing hydrogenase alpha chain vhuA [similarity] -
Methanococcus voltae >gi|1747408|emb|CAA43510.1|
(X61204) methylviologen-reducing hydrogenase subunit
[Methanococcus voltae]
Length = 420
Score = 157 bits (393), Expect = 2e-37
Identities = 122/420 (29%), Positives = 206/420 (49%), Gaps = 30/420 (7%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVK-EVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
IT+ + R+EG G V I + D G +VKL+I R FE IG+ EE I PRIC
Sbjct: 4 ITIAPLTRLEGHGKVTIKLDDSGKPADVKLHIT-ALRGFEQFVIGRPAEEVPRIVPRICG 62
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYL----- 121
C AH L +++A + A G + RE++++G+MI SHALH Y L PD++
Sbjct: 63 ICQTAHHLASVKAVDAAWGAQIPSAAEKQRELMHLGNMIHSHALHFYYLAAPDFVLGPDA 122
Query: 122 --GYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLE 179
+ + +++ + + A+ ++ +G M++ G + IH I GG K S+E +
Sbjct: 123 DPAIRNIVGVIDAAPEVAKKAIAMRRVGQSMVEATGGKPIHPVTGIPGGLSKSMSEEKRD 182
Query: 180 EMKAKLRESLSLAEYTFELFAKL-EQYREVEGEITHLAVKPRGDV----YGIYGDYIK-- 232
E+ A++ + + +L L E+Y + + + G V + YGD ++
Sbjct: 183 ELLAEIDTMIQYGQDGLDLMKSLNEKYLDTINSLGVIDTWYLGLVKDGKHNFYGDTLRFV 242
Query: 233 ---ASDGEEFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKDLLY 284
S+ EF +Y +++ E VVEHS+ K+ + K G P + VG ++ + +
Sbjct: 243 SPDGSEKMEFKPAEYLDYLGEHVVEHSYVKYPYNKKVGYPEGLYRVGPLAMINVCDSMST 302
Query: 285 GRAKDLYESHKELL--KGTNPFANNLAQALELVYFIERAIDLI-DEVLIKWPVKERDKVE 341
A++ + E A N A+ +EL+ ERA +L+ D ++ VK +VE
Sbjct: 303 PLAEEARKEFAETFGRPANQSIAYNQARLIELLSACERAKELLEDPEIVSTDVKA--EVE 360
Query: 342 VRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDD 400
+ G GV APRG L + + +NG V A++I T N+ ME+ ++ AE + D+
Sbjct: 361 PKAGNGVGVVYAPRGTLFHNYETDDNGIVTKANMIVATTHNVPTMEKAIQQAAEVLFKDN 420
>gi|11498968 methylviologen-reducing hydrogenase, subunit alpha (vhuA)
[Archaeoglobus fulgidus] >gi|7447185|pir||C69421
methylviologen-reducing hydrogenase, subunit alpha
(vhuA) homolog - Archaeoglobus fulgidus
>gi|2649200|gb|AAB89872.1| (AE001009)
methylviologen-reducing hydrogenase, subunit alpha
(vhuA) [Archaeoglobus fulgidus]
Length = 458
Score = 146 bits (364), Expect = 6e-34
Identities = 124/437 (28%), Positives = 201/437 (45%), Gaps = 35/437 (8%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICSF 67
I ++ ++R+EG V I + G E R +E I G +EE + IC
Sbjct: 3 IEINPVSRIEGHAKVTIFLDSSGKVENAFFQATEFRGYEKILSGLPMEEVPRVVSTICGI 62
Query: 68 CSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYL-----G 122
C A H AL+AA++ G + + +RE+L IE H L L L LPD+L
Sbjct: 63 CRAIHFTAALKAADQVFGVEASDAAKIVREMLLCAHFIEDHTLSLLALALPDFLSPEERN 122
Query: 123 YSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMK 182
++ + E +EL L+ + ++ +LG R IH A+ GG+ K P+ E L ++
Sbjct: 123 IFGVIRNLGELGREL---LRKRGYAVRILQILGGRNIHPVTALPGGWAKAPTDEELGLIE 179
Query: 183 AKLRESLSLAEYTFE-LFAKLEQYREVEGEITHLAVKPRGDVYGIYGD-YIKASDGEE-- 238
RE L L E L A L+ +E L + +G V G+ +K +G+E
Sbjct: 180 RYSRELLDLGYRMVEVLEAALDMGDGLELRAATLGTEKKGLVNLYDGEQVVKDEEGKEVL 239
Query: 239 -FPSEDYKEHINEFVVEHSFAKHSHYK---GKPFMVGAISRVVNNKDLLYGRAKDLYESH 294
F ++Y + I E + S++K + K K ++VG ++R+ + +L
Sbjct: 240 RFSGKEYADIITEKSLSWSYSKLTEIKPLGEKLYVVGPLARL---------KTAELTPKA 290
Query: 295 KELLK----GTNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVST 350
E++K N N+ A+A+E++Y ER ++L E KV G GV
Sbjct: 291 SEIVKTYGVSNNLLYNHFARAVEIIYAAERLLELSQSYRGGRIQNEPQKVA---GEGVGI 347
Query: 351 TEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAE 409
EAPRG L++ E G V +++I T N+ + V +AE+ D L E
Sbjct: 348 VEAPRGTLIHHYWTDEKGIVRGSNLIVATTHNVRAINHVVGKVAER--ASDTRNLAEKVE 405
Query: 410 MVVRAYDPCISCSVHVV 426
+ +RA+DPC++C+ H V
Sbjct: 406 VAIRAFDPCMACATHAV 422
>pir||S16727 coenzyme F420 hydrogenase (EC 1.12.99.1) alpha chain vhcA
[similarity] - Methanococcus voltae
>gi|44794|emb|CAA43506.1| (X61203) vhcA [Methanococcus
voltae]
Length = 474
Score = 135 bits (337), Expect = 8e-31
Identities = 121/453 (26%), Positives = 210/453 (45%), Gaps = 37/453 (8%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
++++ + RVEG G V + D G + +V +++E R FE G+ +E+A PRIC
Sbjct: 4 LSIEPVTRVEGHGKVTLSFDDSGKLDKVNFHVVE-VRGFEKFLEGRYIEDAPIFTPRICG 62
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSP 126
C +H L + +A + G E LR +++ + SHALH +L PD + ++
Sbjct: 63 ICQVSHHLASAKAVDNVFGVKIPETANMLRNLIHQASNVHSHALHFGMLASPDLMFPTTD 122
Query: 127 -------LKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLE 179
L + E ++ ++ ++ +G ++ +G RAIH AI+GG K ++E +
Sbjct: 123 DALKRNLLGVAGENMDMIKDSIAMRKVGQTIVQKVGGRAIHPVTAIVGGQSKPLTEEERD 182
Query: 180 EMKAKLRESLSLAEYTFELFAKL-EQYREVE------GEITHLAVKPRGDVYGIYGDYIK 232
E+ + AE T + ++ E +E + E H+ + G IY I+
Sbjct: 183 ELLTLSDNLVETAERTLNVGKEMVENLKEKDLMNLGYFESYHMGLVNNG-AQDIYEGNIR 241
Query: 233 ASDGE-----EFPSEDYKEHINEFVVEHSFAK-----HSHYKGK-PFMVGAISRVVNNKD 281
+ E EF +Y +I E V +S+ K +GK + V +SR+
Sbjct: 242 VVNSEGKIEYEFDPAEYANYITEGVRPYSYLKFLALLKRRGEGKGAYRVNTLSRLNVCDK 301
Query: 282 LLYGRAKDLYESHKELL--KGTNPFANNLAQALELVYFIERAIDLI-DEVLIKWPVKERD 338
+ A+ YE + P + A+ +EL+ E + D+ ++ V+
Sbjct: 302 MPTPIAQKYYEEFVKTYGKPAHQPMLFHYARLIELLSSSELIRKFLEDDKIVDTDVRAEV 361
Query: 339 KVEVRDGFGVSTTEAPRGILVYALKVEN-GRVAYADIITPTAFNLAMMEEHVRMMAEKHY 397
+ G GV EAPRG L++ K ++ G + +++ T N M+ V+ +AEK Y
Sbjct: 362 DPKNITGEGVGCVEAPRGTLIHHFKTDDRGIINDTNLVVATVQNNPAMDIGVQKVAEK-Y 420
Query: 398 NDDPERLK--LL--AEMVVRAYDPCISCSVHVV 426
PE K +L EMV+RAYDPC+SC+ H +
Sbjct: 421 IKTPEVAKPHILNHMEMVIRAYDPCLSCATHTI 453
>pir||S75530 hydrogenase large chain - Synechocystis sp. (strain PCC 6803)
>gi|1653175|dbj|BAA18091.1| (D90911) hydrogenase large
subunit [Synechocystis sp.] >gi|1771720|emb|CAA66212.1|
(X97610) hydrogenase subunit [Synechocystis sp.]
Length = 474
Score = 134 bits (334), Expect = 2e-30
Identities = 120/451 (26%), Positives = 216/451 (47%), Gaps = 40/451 (8%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
I +D + R+EG + I + D+G V +V+ +++E R FE G+ + E I RIC
Sbjct: 5 IVIDPVTRIEGHAKISIFLNDQGNVDDVRFHVVEY-RGFEKFCEGRPMWEMAGITARICG 63
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY-LGYSS 125
C +H L A + +K + + LR ++ +G + +SHAL + L PD+ LG+ S
Sbjct: 64 ICPVSHLLCAAKTGDKLLAVQIPPAGEKLRRLMNLGQITQSHALSFFHLSSPDFLLGWDS 123
Query: 126 PLKMVNEY---KKELEIA---LKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLE 179
N + + ++A ++L+ G ++++LG++ IH ++ GG S+E +
Sbjct: 124 DPATRNVFGLIAADPDLARAGIRLRQFGQTVIELLGAKKIHSAWSVPGGVRSPLSEEGRQ 183
Query: 180 EMKAKLRESLSLAEYTFELFAK-LEQYREVEGEI-----THLAVKPRGDVYGIYGDYIKA 233
+ +L E+ LF L++++ E + + + + + YG ++
Sbjct: 184 WIVDRLPEAKETVYLALNLFKNMLDRFQTEVAEFGKFPSLFMGLVGKNNEWEHYGGSLRF 243
Query: 234 SDGE------EFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKDL 282
+D E ++Y + I E V + S+ K +YK G P + VG ++R+ +
Sbjct: 244 TDSEGNIVADNLSEDNYADFIGESVEKWSYLKFPYYKSLGYPDGIYRVGPLARLNVCHHI 303
Query: 283 LYGRAKDLYESHKELLKG--TNPFANNLAQALELVYFIERAIDLI---DEVLIKWPVKER 337
A E +++ G T+ F + A+ +E++ +E AI+L+ ++L K R
Sbjct: 304 GTPEADQELEEYRQRAGGVATSSFFYHYARLVEILACLE-AIELLMADPDILSK---NCR 359
Query: 338 DKVEVRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKH 396
K E+ V +EAPRG L + K+ E+G + ++I T N M + V +A KH
Sbjct: 360 AKAEINCTEAVGVSEAPRGTLFHHYKIDEDGLIKKVNLIIATGNNNLAMNKTVAQIA-KH 418
Query: 397 Y---NDDPERLKLLAEMVVRAYDPCISCSVH 424
Y +D E E +R YDPC+SCS H
Sbjct: 419 YIRNHDVQEGFLNRVEAGIRCYDPCLSCSTH 449
>pir||S74233 bidirectional hydrogenase chain H - Synechococcus sp. (PCC 6301)
>gi|1708772|emb|CAA66383.1| (X97797) hoxH [Synechococcus
PCC6301]
Length = 476
Score = 133 bits (331), Expect = 4e-30
Identities = 114/449 (25%), Positives = 205/449 (45%), Gaps = 35/449 (7%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICSF 67
I +D + R+EG + + + D+G E + R FE G+ E I RIC
Sbjct: 5 IVIDPVTRIEGHAKISVFLDDQGNAEAARFHVVEYRGFEKFCEGRPFTEMAGITARICGI 64
Query: 68 CSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY-LGYSSP 126
C +H L A + +K + + LR ++ + +I+SHAL + L PD+ LG+ S
Sbjct: 65 CPVSHLLAAAKTGDKILAVQIPPAAENLRRLINLAQIIQSHALSFFHLSSPDFLLGWDSD 124
Query: 127 LKMVNEY------KKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEE 180
N + + ++L+ G +++ LG R IH A+ GG L S+E +
Sbjct: 125 PARRNLFGLIAADPESARAGIRLRQFGQQLIEWLGGRKIHAAWAVPGGVRSLLSQEGMVW 184
Query: 181 MKAKLRESLSLAEYTFELFAKL--EQYRE---VEGEITHL---AVKPRGDVYGIYGDYIK 232
++ +L L+ T + F +L ++RE V G+ L V GD + YG +++
Sbjct: 185 IRDRLPAELATVRATLDRFKRLLDREFREETAVFGDFPSLFMGLVADNGD-WEHYGGHLR 243
Query: 233 ASD------GEEFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNKD 281
D + +DY + E V S+ K +YK G P + VG ++R+
Sbjct: 244 FVDSQGRIIADRLREDDYASFLGEAVEPWSYLKFPYYKPWGYPEGMYRVGPLARLNVCDR 303
Query: 282 LLYGRAKDLYESHKELLKG--TNPFANNLAQALELVYFIERAIDLIDEVLIKWP-VKERD 338
+ GR G T+ F + A+ +E++ +E+ +++++ ++ ++ +
Sbjct: 304 IGTGRGGSRATGTARSRGGTVTSSFLYHYARLVEILASLEKIAEMVEDPNLQTGFLRSQA 363
Query: 339 KVEVRDGFGVSTTEAPRGILVYALKVE-NGRVAYADIITPTAFNLAMMEEHVRMMAEKH- 396
V + GVS EAPRG L + +V+ +G++ ++I T N M V +A+ +
Sbjct: 364 GVNCLEAIGVS--EAPRGTLFHHYRVDSHGKIERVNLIIATGQNNLAMNRTVTQIAQHYI 421
Query: 397 -YNDDPERLKLLAEMVVRAYDPCISCSVH 424
+ + E E +R +DPC+SCS H
Sbjct: 422 RHGEVQESFLNRVEAGIRCFDPCLSCSTH 450
>pir||S72553 hydrogenase large chain - Anabaena variabilis
>gi|2126555|pir||I39735 hydrogenase large chain -
Anabaena variabilis >gi|1032481|emb|CAA55878.1| (X79285)
hydrogenase large subunit [Anabaena variabilis]
Length = 487
Score = 130 bits (323), Expect = 4e-29
Identities = 127/458 (27%), Positives = 210/458 (45%), Gaps = 45/458 (9%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
I +D + R+EG + I + D G V + + ++ E R FE G+ L E I RIC
Sbjct: 5 IVIDPVTRIEGHAKISIYLDDTGQVSDARFHVTEF-RGFEKFCEGRPLWEMPGITARICG 63
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPD-YLGYSS 125
C +H L + +A ++ + T LR ++ +G +++SHAL + L PD LG S
Sbjct: 64 ICPVSHLLASAKAGDRILSVTIPPTATKLRRLMNLGQILQSHALSFFHLTAPDLLLGMDS 123
Query: 126 PLKMVNEY---KKELEIA---LKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLE 179
+ N + + E+A ++L+ G +++VLG IH A+ GG + S E
Sbjct: 124 DPQKRNIFGLIAAQPELARGGIRLRQFGQEIIEVLGGAKIHPAWAVPGGVREPLSVEGRT 183
Query: 180 EMKAKLRESLSLAEYTFELFAKLEQYREVE----GEITHL---AVKPRGDVYGIYGDYIK 232
++ ++ E+ ++A + F KL + E E G L V P G ++ Y YI+
Sbjct: 184 HIQERIPEARTIALDALDRFKKLLKDYEKEVQTFGNFPSLFMGLVTPDG-LWETYDGYIR 242
Query: 233 ASD------GEEFPSEDYKEHINEFVVEHSFAKHSHYK--GKP------------FMVGA 272
D ++ Y+E I E V S+ K +Y+ G P + VG
Sbjct: 243 FVDSAGNIIADKLDPARYQEFIGEAVQPDSYLKSPYYRPLGYPDQHDQCRIDSGMYRVGP 302
Query: 273 ISRVVNNKDLLYGRAKDLYESHKELLKGT--NPFANNLAQALELVYFIERAIDLIDEVLI 330
++R+ + A +EL GT + F + A+ +E++ IE L+D+ I
Sbjct: 303 LARLNICSHIGTTLADRELREFRELTSGTAKSSFFYHYARLIEILACIEHIEMLLDDPDI 362
Query: 331 KWPVKERDKVEVRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHV 389
+ R + V V +EAPRG L + +V ENG + ++I T N M V
Sbjct: 363 -LSNRLRSEAGVNQLEAVGVSEAPRGTLFHHYQVDENGLLQKVNLIIATGQNNLAMNRTV 421
Query: 390 RMMAEKHY---NDDPERLKLLAEMVVRAYDPCISCSVH 424
+A +H+ + PE + E +RA+DPC+SCS H
Sbjct: 422 AQIA-RHFIQGTEIPEGMLNRVEAGIRAFDPCLSCSTH 458
>sp|Q60338|FRHA_METJA COENZYME F420 HYDROGENASE ALPHA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE ALPHA
SUBUNIT) (FRH) >gi|2127832|pir||A64299 coenzyme F420
hydrogenase (EC 1.12.99.1) alpha chain [similarity] -
Methanococcus jannaschii
Length = 415
Score = 130 bits (323), Expect = 4e-29
Identities = 114/432 (26%), Positives = 202/432 (46%), Gaps = 55/432 (12%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGP-RFFEAITIGKKLEEALAIYPRICS 66
I + R EG + + V +EG+ + P R FE + GK E A RIC
Sbjct: 10 IEIAPTTRHEGHAKLILEVDEEGIVNKAYYLNTTPVRGFETMLKGKPAEFAPIAVMRICG 69
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSP 126
C H + + EA E AI ++ LRE++ IG+ + SH LH +LL + D+L
Sbjct: 70 ICQTTHGIASCEAIENAIDCEVPDDGLLLRELVGIGNRLHSHPLH-HLLTIDDFLKPDE- 127
Query: 127 LKMVNEYKKEL-EIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKL 185
+ K EL ++ +++ +G ++D++G IH N ++GG T +AK
Sbjct: 128 ----TDLKIELIKLIQRMRKVGQLVVDIVGGEGIHPPNIVIGGM------RTNITERAKS 177
Query: 186 RESLSLAEYTFELFAKLEQYRE-VEGEITHLAVKPRG-------DVYGIYGD--YIKASD 235
R +L +Y + + E+Y E +E + + + G + YGD I D
Sbjct: 178 RLYYALRQYEKDAYELYEKYTELIERYLEEIGIPDLGAHEYPYIATHTTYGDRYAINWDD 237
Query: 236 GEEFPSEDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHK 295
E P++ Y + +E + + + Y G P G +R+V +G ++
Sbjct: 238 VTEIPAQRYYD--DEEAKQTTTIQIPLYAGVPAEGGPRARMVK-----FGNFRE------ 284
Query: 296 ELLKGTNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPR 355
G + N+A+A E + + RA++++DE+ + K R +VE +DGFG+ EAPR
Sbjct: 285 ----GGSAMDINIARAQENLGAVYRALEILDELDLNG--KTRAEVEYKDGFGIGVHEAPR 338
Query: 356 GILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRA 414
+ +V ++G++ II + +N ++E+ + +++ AE+++RA
Sbjct: 339 ATNTHMAEVGKDGKIKSYRIIAASTWNFPIVEKAIEGYPQQY-----------AEVIMRA 387
Query: 415 YDPCISCSVHVV 426
YD C SC+ HV+
Sbjct: 388 YDICASCATHVI 399
>sp|Q00390|FRHA_METVO COENZYME F420 HYDROGENASE ALPHA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE ALPHA
SUBUNIT) (FRH) >gi|99140|pir||S16721 coenzyme F420
hydrogenase (EC 1.12.99.1) alpha chain frcA [similarity]
- Methanococcus voltae >gi|44701|emb|CAA43496.1|
(X61201) F420-reducing hydrogenase subunit
[Methanococcus voltae]
Length = 398
Score = 128 bits (318), Expect = 1e-28
Identities = 115/425 (27%), Positives = 190/425 (44%), Gaps = 49/425 (11%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGP-RFFEAITIGKKLEEALAIYPRICS 66
+ ++ R EG + + V D+G+ E + P R FE +GK E A R C
Sbjct: 5 VEINPTTRHEGHTKLVLKVDDDGIVEKGNYLSVTPVRGFEKFLVGKPAEFAPIAVSRFCG 64
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSP 126
C AH +A+EA E A G P ++ LRE+ +G+ + SH LH + L+ PD++ P
Sbjct: 65 ICPIAHATSAVEAIEDACGIVPPKDGLLLRELTGLGNKMHSHPLHEF-LIAPDFI----P 119
Query: 127 LKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKLR 186
K EY ++ +++ G +++D +G AIH N +GG K + + ++ K +
Sbjct: 120 EKDRVEYITRIQ---QMRKTGQYIVDTIGGEAIHAPNIKVGGMLKSITPSAVSKIYYKCK 176
Query: 187 ESLSLAEYTFELFAKLEQYRE-VEGEITHLAVKPRGDVYGIYGDYIKASDGEEFPSEDYK 245
E LA+ E + + R V+G T + K +G + E +
Sbjct: 177 EFEKLAKEQVEYLIPIFENRTLVDG--TEIPEKLGYHDFGYLATDSTYGNRENIEQKKVH 234
Query: 246 EHINEFVVEHSFAKHS-----HYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKG 300
E+ V E A + Y G+ VG +R D K
Sbjct: 235 EYTPYDVYEKEVAVQACQLFQEYNGRLMEVGPRARFAKFHD----------------FKE 278
Query: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDG--FGVSTTEAPRGIL 358
A ++A+A E V ++RA+++I+E+ + + +D + + DG G+ EA RG
Sbjct: 279 KGAMAIHIARAYENVIHVKRAMEIIEELNVDGLTRAKDPI-LGDGEKLGLGVHEAARGHN 337
Query: 359 VYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDP 417
+ + E GR+ Y + I T +N+ ++ + V E HY AE VVRAYDP
Sbjct: 338 THQASIDEKGRITYYNAIVATTWNIPLIGKAV----EAHYK--------FAEHVVRAYDP 385
Query: 418 CISCS 422
C+SC+
Sbjct: 386 CVSCA 390
>gb|AAB53705.1| (U88400) bidirectional hydrogenase large subunit [Prochlorothrix
hollandica]
Length = 482
Score = 121 bits (300), Expect = 2e-26
Identities = 116/450 (25%), Positives = 205/450 (44%), Gaps = 37/450 (8%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKE--VKLNIIEGPRFFEAITIGKKLEEALAIYPRIC 65
I +D + R+EG + I + D G + + +++E R FE G+ E I RIC
Sbjct: 5 IVIDPVTRIEGLAKISIFLDDAGKVDDAARFHVVEY-RGFEKFCEGRAYTEMAGITARIC 63
Query: 66 SFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDY-LGYS 124
C +H L A + +K + + LR ++ + + +SHAL + L PD+ LG+
Sbjct: 64 GICPVSHLLAAAKTGDKILAVKVPIAAEKLRRLMNLAQLTQSHALSFFHLSSPDFLLGWD 123
Query: 125 SP------LKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETL 178
S +++ ++L+ G ++++LG+R IH + GG ++E+
Sbjct: 124 SDPARRNVFGLIDANPDLARAGIRLRQFGQTIIELLGARKIHAAWTVPGGVRSPLTEESR 183
Query: 179 EEMKAKLRESLSLAEYTFELF-----AKLEQYREVEGEITHL--AVKPRGDVYGIYGDYI 231
+ + +L ES + + +LF L+ + GE L ++ + YG ++
Sbjct: 184 QWILDRLPESFATLQTALDLFKGLLDKALKDEVGIFGEFPSLFMSLVAADGAWEHYGGHL 243
Query: 232 KASDG------EEFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVGAISRVVNNK 280
+ DG + ++Y + + E V + S+ K +Y+ G P + VG ++R+
Sbjct: 244 RFVDGQGTIVADNLSEDNYLDFLGEGVEKWSYLKFPYYRPLGYPAGIYRVGPLARLNTCS 303
Query: 281 DLLYGRA-KDLYE-SHKELLKGTNPFANNLAQALELVYFIERAIDLI-DEVLIKWPVKER 337
+ RA +L E S + T+ F + A+ +E++ +E+ L+ D L+ +
Sbjct: 304 HIGTPRADAELGEMSPRFHAPVTSSFLYHYARLIEILACLEKIHHLMADPDLLSNRCRAT 363
Query: 338 DKVEVRDGFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKH 396
V + GVS EAPRG L + KV ENG + ++I T N M + V +A KH
Sbjct: 364 GGVNELEAVGVS--EAPRGTLFHHYKVDENGLIEKVNLIIATGQNNLAMNKTVTQIA-KH 420
Query: 397 YNDDPERLKLL--AEMVVRAYDPCISCSVH 424
Y L E + YDPC+SCS H
Sbjct: 421 YPRSRYSRSFLNRVEAGIPCYDPCLSCSTH 450
>sp|P22320|HOXH_ALCEU NAD-REDUCING HYDROGENASE HOXS BETA SUBUNIT >gi|95477|pir||D35385
hydrogen dehydrogenase (EC 1.12.1.2) beta chain,
cytosolic - Alcaligenes eutrophus
>gi|141950|gb|AAC06143.1| (M55230) NAD-reducing
hydrogenase [Ralstonia eutropha]
Length = 488
Score = 119 bits (296), Expect = 5e-26
Identities = 114/468 (24%), Positives = 211/468 (44%), Gaps = 55/468 (11%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
+ +D + R+EG G V + + D+ V + KL+++E R FE G EA RIC
Sbjct: 5 LVIDPVTRIEGHGKVVVHLDDDNKVVDAKLHVVEF-RGFEKFVQGHPFWEAPMFLQRICG 63
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEI------QALREVLYIGDMIESHALHLYLLVLPDY 120
C +H L +A + +G + I + +R + + M++SH + L++P+
Sbjct: 64 ICFVSHHLCGAKALDDMVGVGLKSGIHVTPTAEKMRRLGHYAQMLQSHTTAYFYLIVPEM 123
Query: 121 L-GYSSP------LKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGK-- 171
L G +P L ++ ++ + L+ G ++ + + +H N++ GG
Sbjct: 124 LFGMDAPPAQRNVLGLIEANPDLVKRVVMLRKWGQEVIKAVFGKKMHGINSVPGGVNNNL 183
Query: 172 -LPSKETL---EEMKAKLRESLSLAEYTFELFAKLEQYREVE----GEITHLAVKPRGDV 223
+ ++ EE + + + A+ LF Q + ++ L++ GD
Sbjct: 184 SIAERDRFLNGEEGLLSVDQVIDYAQDGLRLFYDFHQKHRAQVDSFADVPALSMCLVGDD 243
Query: 224 YGI--YGDYIKASDGE-----EFPSEDYKEHINEFVVEHSFAKHSHYK--GKP---FMVG 271
+ Y ++ D + EF DY +H +E V E S+ K + K G+ VG
Sbjct: 244 DNVDYYHGRLRIIDDDKHIVREFDYHDYLDHFSEAVEEWSYMKFPYLKELGREQGSVRVG 303
Query: 272 AISRVVNNKDLLYGRAKDLYESHKELLKG-TNPFA--NNLAQALELVYFIERAIDLIDEV 328
+ R+ K L A++ E KG TN N A+A+E+++ E +L+ +
Sbjct: 304 PLGRMNVTKSLPTPLAQEALERFHAYTKGRTNNMTLHTNWARAIEILHAAEVVKELLHD- 362
Query: 329 LIKWPVKERDKVEVRD------GFGVSTTEAPRGILVYALKV-ENGRVAYADIITPTAFN 381
P ++D++ + G GV EAPRG L++ + E G + +A+++ T N
Sbjct: 363 ----PDLQKDQLVLTPPPNAWTGEGVGVVEAPRGTLLHHYRADERGNITFANLVVATTQN 418
Query: 382 LAMMEEHVRMMAEKH---YNDDPERLKLLAEMVVRAYDPCISCSVHVV 426
+M VR +AE + + + E + E+ +RAYDPC+SC+ H +
Sbjct: 419 HQVMNRTVRSVAEDYLGGHGEITEGMMNAIEVGIRAYDPCLSCATHAL 466
>sp|P19496|FRHA_METTH COENZYME F420 HYDROGENASE ALPHA SUBUNIT
(8-HYDROXY-5-DEAZAFLAVIN-REDUCING HYDROGENASE ALPHA
SUBUNIT) (FRH) >gi|80912|pir||A35620 coenzyme F420
hydrogenase (EC 1.12.99.1) alpha chain -
Methanobacterium thermoautotrophicum
>gi|551889|gb|AAA72187.1| (J02914) F420 hydrogenase
alpha-subunit (frhA) [Methanobacterium
thermoautotrophicum]
Length = 405
Score = 119 bits (295), Expect = 7e-26
Identities = 108/425 (25%), Positives = 185/425 (43%), Gaps = 47/425 (11%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
I + +R EG + + V DEG V + + I R E I GK E A I RIC
Sbjct: 5 IVISPTSRQEGHAELVMEVDDEGIVTKGRYFSITPVRGLEKIVTGKAPETAPVIVQRICG 64
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSP 126
C H L ++EA + ++ + + LRE+ + SHA+H + L+ PD++ +
Sbjct: 65 VCPIPHTLASVEAIDDSLDIEVPKAGRLLRELTLAAHHVNSHAIH-HFLIAPDFVPENLM 123
Query: 127 LKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKLR 186
+N +++ +++D++ IH + +GG ++ + + A+L+
Sbjct: 124 ADAINSVS-------EIRKNAQYVVDMVAGEGIHPSDVRIGGMADNITELARKRLYARLK 176
Query: 187 ESLSLAEYTFELFAKLEQYREVEGEITHLAV--KPRGDVYGIYGDYIKAS---DGEEFPS 241
+ + EL L E +G L V +P + IYGD K E P
Sbjct: 177 QLKPKVDEHVELMIGL---IEDKGLPKGLGVHNQPTLASHQIYGDRTKFDLDRFTEVMPE 233
Query: 242 EDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGT 301
Y + + + + + Y G+ VG +R+V ++ KE
Sbjct: 234 SWYDD---PEIAKRACSTIPLYDGRNVEVGPRARMVE------------FQGFKE----R 274
Query: 302 NPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVYA 361
A ++A+ALE+ + RAI+++DE+ PV+ GV E PRG+ V+
Sbjct: 275 GVVAQHVARALEMKTALARAIEILDELDTSAPVRADFDERGTGKLGVGAIEGPRGLDVHM 334
Query: 362 LKVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCISC 421
+VENG++ + + PT +N+ M H+ P V+RAYDPC+SC
Sbjct: 335 AQVENGKIQFYSALVPTTWNIPTMGPATEGF---HHEYGPH--------VIRAYDPCLSC 383
Query: 422 SVHVV 426
+ HV+
Sbjct: 384 ATHVM 388
>gb|AAB85780.1| (AE000895) coenzyme F420-reducing hydrogenase, alpha subunit
[Methanobacterium thermoautotrophicum]
Length = 408
Score = 119 bits (295), Expect = 7e-26
Identities = 108/425 (25%), Positives = 185/425 (43%), Gaps = 47/425 (11%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
I + +R EG + + V DEG V + + I R E I GK E A I RIC
Sbjct: 8 IVISPTSRQEGHAELVMEVDDEGIVTKGRYFSITPVRGLEKIVTGKAPETAPVIVQRICG 67
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSP 126
C H L ++EA + ++ + + LRE+ + SHA+H + L+ PD++ +
Sbjct: 68 VCPIPHTLASVEAIDDSLDIEVPKAGRLLRELTLAAHHVNSHAIH-HFLIAPDFVPENLM 126
Query: 127 LKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKLR 186
+N +++ +++D++ IH + +GG ++ + + A+L+
Sbjct: 127 ADAINSVS-------EIRKNAQYVVDMVAGEGIHPSDVRIGGMADNITELARKRLYARLK 179
Query: 187 ESLSLAEYTFELFAKLEQYREVEGEITHLAV--KPRGDVYGIYGDYIKAS---DGEEFPS 241
+ + EL L E +G L V +P + IYGD K E P
Sbjct: 180 QLKPKVDEHVELMIGL---IEDKGLPKGLGVHNQPTLASHQIYGDRTKFDLDRFTEVMPE 236
Query: 242 EDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGT 301
Y + + + + + Y G+ VG +R+V ++ KE
Sbjct: 237 SWYDD---PEIAKRACSTIPLYDGRNVEVGPRARMVE------------FQGFKE----R 277
Query: 302 NPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVYA 361
A ++A+ALE+ + RAI+++DE+ PV+ GV E PRG+ V+
Sbjct: 278 GVVAQHVARALEMKTALARAIEILDELDTSAPVRADFDERGTGKLGVGAIEGPRGLDVHM 337
Query: 362 LKVENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCISC 421
+VENG++ + + PT +N+ M H+ P V+RAYDPC+SC
Sbjct: 338 AQVENGKIQFYSALVPTTWNIPTMGPATEGF---HHEYGPH--------VIRAYDPCLSC 386
Query: 422 SVHVV 426
+ HV+
Sbjct: 387 ATHVM 391
>emb|CAA43500.1| (X61202) F420-reducing hydrogenase subunit [Methanococcus voltae]
Length = 411
Score = 116 bits (289), Expect = 3e-25
Identities = 110/431 (25%), Positives = 195/431 (44%), Gaps = 49/431 (11%)
Query: 7 PITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGP-RFFEAITIGKKLEEALAIYPRIC 65
PIT+ R +G + + V D+G+ P R FE + GK RIC
Sbjct: 4 PITIAPTTRHKGHAKLVLNVDDDGIVTKAFYPNTTPVRGFETMLQGKPAAFGPIAVMRIC 63
Query: 66 SFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSS 125
C A H + + EA E AIG ++ LRE+L +G+ + SH LH +LL++ D L
Sbjct: 64 GICQATHGIASCEAIENAIGAEIPQDGAILRELLGLGNRMHSHPLH-HLLIVDDLL---K 119
Query: 126 PLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKL 185
P + +++ K++ G ++D++G IH N ++GG ++ ++
Sbjct: 120 PEEADTLRIDGIKLIQKMRKEGQLIVDIVGGEGIHPPNLVIGGMRNNITERAKSKLYYAS 179
Query: 186 RESLSLAEYTFELFAKL-EQYREVEG-------EITHLAVKPRGDVYGIYGDYIKASDGE 237
+E A ++L L E+Y + G ++ ++A D D +K D
Sbjct: 180 KEYQEDARQMYDLIKMLTERYLDEIGIPDLGRHDLPYIAT----DTTFGNRDNLKLDDIA 235
Query: 238 EFPSEDYKEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVN-NKDLLYGRAKDLYESHKE 296
E +++Y + E + + + + Y G P G +R+V G D+
Sbjct: 236 ELTAQNYYSNYPE-IAQTATNQIPLYLGTPAEGGPRARMVKFGSFRTDGGVMDV------ 288
Query: 297 LLKGTNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRG 356
NLA+ALE + +ER+++L++E+ I+ K E +DGFGV EAPR
Sbjct: 289 ----------NLARALENLGAVERSLELLEELNIEGATKITP--EYKDGFGVGVHEAPRA 336
Query: 357 ILVYALKV-ENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAY 415
+ ++ +GR+ II + +N+ ++E+ + K+ AE+++RAY
Sbjct: 337 TNTHMAEMGADGRIKNYRIIAASTWNMPVVEKAIEGYHHKY-----------AEVIMRAY 385
Query: 416 DPCISCSVHVV 426
D SC+ H++
Sbjct: 386 DIXASCATHII 396
>gb|AAB57892.1| (U70364) NAD-reducing hydrogenase beta subunit [Rhodococcus opacus]
Length = 488
Score = 114 bits (283), Expect = 2e-24
Identities = 113/469 (24%), Positives = 206/469 (43%), Gaps = 57/469 (12%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICS 66
+ +D + R+EG G V + + D V + L+++E R FE + G EA + RIC
Sbjct: 5 LVIDPVTRIEGHGKVTVHLDDNNNVVDAHLHVVEF-RGFEKLVQGHPFWEAPMLMQRICG 63
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEI------QALREVLYIGDMIESHALHLYLLVLPDY 120
C +H L +A + +G + I + +R + + M++SHA + L++P+
Sbjct: 64 ICFVSHHLCGAKALDDMVGVGLKSGIDVTPTAEKIRRLGHYAQMLQSHATAYFYLIVPEM 123
Query: 121 L-------GYSSPLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLP 173
L + L ++ + ++ + L+ G ++ + R +H +++ GG K
Sbjct: 124 LFGMDAAPEQRNVLGLIEANPELVKRVVMLRKWGQEVIKAVFGRRMHGISSVPGGVNKNL 183
Query: 174 SKETL------EEMKAKLRESLSLAEYTFELFAKLEQYREVEGE-------ITHLAVKPR 220
S EE + E + A+ +LF + V+ + ++ V
Sbjct: 184 SVAECQRFLKGEEGLPSVDEVIEYAQEGVQLFYDFHEQNRVQVDSFANVSALSMSLVDAD 243
Query: 221 GDVYGIYGDYIKASDG----EEFPSEDYKEHINEFVVEHSFAKHSHYKGK-----PFMVG 271
G+V +G D +EF DY +H +E V E S+ K K VG
Sbjct: 244 GNVDYYHGKLRIIDDDKNVVQEFDYHDYLDHFSEAVEEWSYMKFPFLKALGRERGSVRVG 303
Query: 272 AISRVVNNKDLLYGRAKDLYESHKELLKGTNPFANNL------AQALELVYFIERAIDLI 325
+ R+ L A++ E TN ANN+ A+A+E+++ E +L+
Sbjct: 304 PLGRLNVTNSLSTPLAQEALERFHAY---TNGKANNMTLHTNWARAIEILHAAELIKELL 360
Query: 326 DEVLIKWPVKERDKVEVRD----GFGVSTTEAPRGILVYALKVEN-GRVAYADIITPTAF 380
++ ++ KE+ + D G GV EAPRG L++ + + G + +A+++ T
Sbjct: 361 NDPDLQ---KEQLLLTPADNAWTGEGVGVVEAPRGTLLHHYRADQEGDITFANLVVATTQ 417
Query: 381 NLAMMEEHVRMMAEKHYNDD---PERLKLLAEMVVRAYDPCISCSVHVV 426
N +M VR +AE + E + E+ +RAYDPC+SC+ H +
Sbjct: 418 NNQVMNRTVRSVAEDYLGGQGEVTEGMMNAIEVGIRAYDPCLSCATHAL 466
>emb|CAA74094.1| (Y13764) F420-reducing hydrogenase isoenzyme I alpha subunit
[Methanosarcina barkeri]
Length = 456
Score = 113 bits (279), Expect = 5e-24
Identities = 113/473 (23%), Positives = 202/473 (41%), Gaps = 91/473 (19%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGP-RFFEAITIGKKLEEALAIYPRICS 66
+ + R EG + + V DEG+ E + P R E + IGK +++ I R+C
Sbjct: 5 VEISPTTRHEGHSKLTLKVNDEGIVERGDWLSTTPVRGIEKLAIGKTMDQVPKIASRVCG 64
Query: 67 FCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSP 126
C AH L +EA E +IG ++ + LR +L+ + + SHALH +L+LPD+ +
Sbjct: 65 ICPIAHTLAGIEAMEASIGCEIPKDAKLLRVILHAANRLHSHALH-NILILPDFYIPDTE 123
Query: 127 LKMVNEYKKELEI------ALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEE 180
K +N + KE + +++ + + V G AIH N +GG + S ++
Sbjct: 124 TK-INPFSKEQPLRSVAVRIFRIREIAQTIGAVAGGEAIHPSNPRVGGMYRNVSSRAKQK 182
Query: 181 MKAKLRESLSLA----EYTFELFAKLE--QYREVEGEITHL---------AVKPRGDVYG 225
+ +E L LA E+ E+ ++ ++ EV G+ L V +YG
Sbjct: 183 IADLAKEGLVLAHEQMEFMIEVIRNMQDREFVEVAGKQIPLPKTLGYHNQGVMATAPMYG 242
Query: 226 IYGDYIKASDGEEFPSEDYKE---------HINEFVVEHSFAKH---------------- 260
+S +E P D+ +++E ++ + +
Sbjct: 243 -------SSSLDEKPMWDFTRWRETRPWDWYMSEETIDLEDSSYPIGGTTKVGTKVNPRM 295
Query: 261 ------SHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGTNPFANNLAQALEL 314
Y G+P VG +R+ K KGT FA ++A+ +E
Sbjct: 296 EACNTVPTYDGQPVEVGPRARLATFKHFTE--------------KGT--FAQHIARQMEY 339
Query: 315 VYFIERAIDLIDEVLIKWPVKERDKVEVRDG-FGVSTTEAPRGILVYALKVENGRVAYAD 373
++ ++ + V D + + +G G + EAPRG V+ +V++G+V +
Sbjct: 340 TDCYYTILNCLENLDTSGKVL-ADTIPLGNGSMGWAANEAPRGTDVHLARVKDGKVLRYE 398
Query: 374 IITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCISCSVHVV 426
++ PT +N R + + +AEMV+RAYDPC+SC+ H++
Sbjct: 399 MLVPTTWNFPTCS---RALTGAPWQ--------IAEMVIRAYDPCVSCATHMI 440
>emb|CAA74090.1| (Y13763) F420-reducing hydrogenase isoenzyme II alpha subunit
[Methanosarcina barkeri]
Length = 456
Score = 110 bits (272), Expect = 3e-23
Identities = 116/474 (24%), Positives = 203/474 (42%), Gaps = 93/474 (19%)
Query: 8 ITVDHIARVEGKGGVEIIVGDEGVKE----VKLNIIEGPRFFEAITIGKKLEEALAIYPR 63
+ + R+EG + + V D+G+ E + + + G E + IGK +E+ I R
Sbjct: 5 VEISPTTRLEGHSKLTLKVDDQGIVERGDWLSITPVRG---IEKLAIGKTMEQVPKIASR 61
Query: 64 ICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGY 123
+C C AH L + EA E +IG + + LR +L+ + I S ALH +L+LPD+
Sbjct: 62 VCGICPIAHTLASTEAMEASIGCEIPTDAKLLRTILHAANRIHSIALH-NILILPDFYIP 120
Query: 124 SSPLK---MVNEYKKELEIA--LKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETL 178
+ K NE +A ++++ + + + G AIH N +GG S
Sbjct: 121 GTEKKFNLFANEQPARSVMARIVRIREIAQTIGAIAGGEAIHPSNPRIGGMYYNVSPRAK 180
Query: 179 EEMKAKLRESLSLA----EYTFELFAKLE--QYREVEGEITHLAVKPRGDVYGIY--GDY 230
++M +E L L E+ F++ ++ ++ EV G+ L K G+
Sbjct: 181 QKMADLAKEGLVLVHEQMEFMFDVIRNMQNREFVEVAGKQIPLPKKLGYHNQGVMATASM 240
Query: 231 IKASDGEEFPSEDYKE---------HINEFVVEHSFAKH--------------------- 260
+S ++ P+ D+ ++ E ++ +
Sbjct: 241 YGSSSLDDNPTWDFTRWKETRPWDWYMGEVTIDLEDPSYPIGGTTKIGTKANPQMEACTG 300
Query: 261 -SHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGTNPFANNLAQALE---LVY 316
Y G+P VG +R+ K+ KGT FA ++A+ +E Y
Sbjct: 301 VPTYDGQPVEVGPRARLATFKNFDE--------------KGT--FAQHIARQMEYPDCCY 344
Query: 317 FIERAIDLID---EVLIKWPVKERDKVEVRDG-FGVSTTEAPRGILVYALKVENGRVAYA 372
I R +D ++ +VL D + DG G + EAPRG ++ +V++G+V +
Sbjct: 345 TILRCLDNLNTSGKVL-------ADHIPQGDGSMGWAANEAPRGSNIHLARVKDGKVLWY 397
Query: 373 DIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCISCSVHVV 426
D++ PT +N R + + +AEMVVRAYDPC+SC+ H++
Sbjct: 398 DMLVPTTWNFPTCS---RALTGAPWQ--------IAEMVVRAYDPCVSCATHMI 440
>pir||A70370 hydrogenase large subunit - Aquifex aeolicus
>gi|2983358|gb|AAC06945.1| (AE000708) hydrogenase large
subunit [Aquifex aeolicus]
Length = 416
Score = 109 bits (269), Expect = 8e-23
Identities = 106/419 (25%), Positives = 194/419 (46%), Gaps = 21/419 (5%)
Query: 13 IARVEGKGGVEIIVGDEGVKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRICSFCSAAH 72
+ RVEG+ + ++ +K+ K++ R E + + +AL I PRIC C AH
Sbjct: 8 LTRVEGEASLNLVWERGVIKDAKISFYS-TRGIEKVLRERPFMDALVINPRICGICGHAH 66
Query: 73 KLTALEAAEKAIGFTP-REEIQALREVLYIGDMIESHALHLYLLVLPDYLGYSSPLKMVN 131
+ + A E AIG E+ + R V I +M+++H YL V+PD+L + L
Sbjct: 67 LIATVRAIENAIGIKEIPEKAKITRLVTQITEMVQNHVKWFYLFVMPDFLKFKESLSQFE 126
Query: 132 EYKKE-LEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKAKLRESLS 190
+K E + A++ + ++ + G + H A+ GG S+ + + ++E +
Sbjct: 127 PFKGERWKRAVQFSSQIVKIIALFGGQWPHSSYAVPGGITSNFSEREVLKALNLVKEGKT 186
Query: 191 LAEYT----FELFAKL-EQYREVE-GEITHLAVKPRGDVYGIYGDYIKASDGEEFPSEDY 244
E ELF L E++ + G+ + + G Y Y K + Y
Sbjct: 187 FFEKNVSEDLELFLNLCEEFNLLSIGKAYNRFLSGGGLAYCFNPSYKKGKGKVCKFNVRY 246
Query: 245 KEHINEFVVEHSFAKHSHYKGKPFMVGAISRVVNNKDLLYGRAKDLYESHKELLKGTNPF 304
+ + ++S A YKG P+ G ++R + +K+ L LY+++ +
Sbjct: 247 VQELE--AADYSKANPVRYKGLPYETGPLARELISKNPL---VLKLYKNYGD--SYAVRV 299
Query: 305 ANNLAQALELVYFIERAI-DLIDEVLIKWPVKERDKVEVRDGFGVSTTEAPRGILVYALK 363
A LA+ +L+ +E+ + +L++ + + E E +GFGV EA RG L++ +
Sbjct: 300 AARLAEIKDLLEILEKLLKELMNHLEEPSCLWEDRSDESGEGFGV--VEAARGTLIHRVV 357
Query: 364 VENGRVAYADIITPTAFNLAMMEEHVRMMAEKHYNDDPERLKLLAEMVVRAYDPCISCS 422
+E G++ +ITP+ +NL + +AEK LK A+MV+R++D C C+
Sbjct: 358 IEKGKIKDYKVITPSQWNLGPRCKKYLGVAEKAIVGLDSELK--AQMVLRSFDLCSVCT 414
>sp|P13065|PHSL_DESBA PERIPLASMIC [NIFESE] HYDROGENASE LARGE SUBUNIT (NIFESE
HYDROGENLYASE LARGE CHAIN)
Length = 514
Score = 107 bits (264), Expect = 3e-22
Identities = 120/497 (24%), Positives = 200/497 (40%), Gaps = 84/497 (16%)
Query: 6 IPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRF--FEAITIGKKLEEALAIYPR 63
+ I++D + RVEG +E+ V D V + K + G F FE I G+ ++ I R
Sbjct: 13 VKISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQR 69
Query: 64 ICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGY 123
IC C AH ++ A + A G + R +++ + ++SH LH Y L DY+
Sbjct: 70 ICGVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKG 129
Query: 124 SSPLKMV---------------------------NEYKKELEIALKLKNLGSWMMDVLGS 156
V N+Y K LEI + + M+ + G
Sbjct: 130 PDVSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEI----RRICHEMVAMFGG 185
Query: 157 RAIHQENAILGGFGKLPSKETLEEMKAKLRE--SLSLAEYTFELFAKLEQYREV------ 208
R H + ++GG ++P+ + + E A+ +E + EY ++ Y ++
Sbjct: 186 RMPHVQGMVVGGATEIPTADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIG 245
Query: 209 EGEITHLAVKPRGDVYGIY----GDYIKASDGEEFPSEDYKEHINEFVVEH--------- 255
+ V P D Y + G YI D EEF S+ KE++ +H
Sbjct: 246 WKNVIAFGVFPEDDDYKTFLLKPGVYIDGKD-EEFDSKLVKEYVGHSFFDHSAPGGLHYS 304
Query: 256 --------------SFAKHSHYKGKPFMVGAISRV-VNNKDLLYGRAKDLYESHKELLKG 300
SF K YK KP VG ++R+ V N +L K L E + K
Sbjct: 305 VGETNPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKK 364
Query: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERD------KVEVRDGF-GVSTTEA 353
+ + + L + KW + + K E+ D G TEA
Sbjct: 365 FRDLGDKAFSIMGRHVLVAEETWLTAVAVEKWLKQVQPGAETYVKSEIPDAAEGTGFTEA 424
Query: 354 PRGILVYALKVENGRVAYADIITPTAFNLAMMEE-HVRMMAEKHYNDDPE---RLKLLAE 409
PRG L++ LK+++ ++ I++ T +N ++ R E+ P + +
Sbjct: 425 PRGALLHYLKIKDKKIENYQIVSATLWNANPRDDMGQRGPIEEALIGVPVPDIKNPVNVG 484
Query: 410 MVVRAYDPCISCSVHVV 426
+VR+YDPC+ C+VHV+
Sbjct: 485 RLVRSYDPCLGCAVHVL 501
>pir||HQDVLB cytochrome-c3 hydrogenase (EC 1.12.2.1) (NiFeSe) large chain -
Desulfovibrio baculatus
Length = 514
Score = 105 bits (260), Expect = 9e-22
Identities = 126/505 (24%), Positives = 210/505 (40%), Gaps = 100/505 (19%)
Query: 6 IPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRF--FEAITIGKKLEEALAIYPR 63
+ I++D + RVEG +E+ V D V + K + G F FE I G+ ++ I R
Sbjct: 13 VKISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQR 69
Query: 64 ICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGY 123
IC C AH ++ A + A G + R +++ + ++SH LH Y L DY+
Sbjct: 70 ICGVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKG 129
Query: 124 SSPLKMV---------------------------NEYKKELEIALKLKNLGSWMMDVLGS 156
V N+Y K LEI + + M+ + G
Sbjct: 130 PDVSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEI----RRICHEMVAMFGG 185
Query: 157 RAIHQENAILGGFGKLPSKETLEEMKAKLRE--SLSLAEYTFELFAKLEQYREV------ 208
R H + ++GG ++P+ + + E A+ +E + EY ++ Y ++
Sbjct: 186 RMPHVQGMVVGGATEIPTADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIG 245
Query: 209 EGEITHLAVKPRGDVYGIY----GDYIKASDGEEFPSEDYKEHINEFVVEH--------- 255
+ V P D Y + G YI D EEF S+ KE++ +H
Sbjct: 246 WKNVIAFGVFPEDDDYKTFLLKPGVYIDGKD-EEFDSKLVKEYVGHSFFDHSAPGGLHYS 304
Query: 256 --------------SFAKHSHYKGKPFMVGAISRV-VNNKDLL---YGRAKDLY--ESHK 295
SF K YK KP VG ++R+ V N +L K+LY E+ K
Sbjct: 305 VGETNPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKK 364
Query: 296 ELLKGTNPFA---NNLAQALELVYFIERAIDLIDEVLIKWPVKERD------KVEVRDGF 346
G F+ ++A+A E + A++ KW + + K E+ D
Sbjct: 365 FRDLGDKAFSIMGRHVARA-EETWLTAVAVE-------KWLKQVQPGAETYVKSEIPDAA 416
Query: 347 -GVSTTEAPRGILVYALKVENGRVAYADIITPTAFNLAMMEE-HVRMMAEKHYNDDPE-- 402
G TEAPRG L++ LK+++ ++ I++ T +N ++ R E+ P
Sbjct: 417 EGTGFTEAPRGALLHYLKIKDKKIENYQIVSATLWNANPRDDMGQRGPIEEALIGVPVPD 476
Query: 403 -RLKLLAEMVVRAYDPCISCSVHVV 426
+ + +VR+YDPC+ C+VHV+
Sbjct: 477 IKNPVNVGRLVRSYDPCLGCAVHVL 501
>pdb|1CC1|L Chain L, Crystal Structure Of A Reduced, Active Form Of The
Ni-Fe-Se Hydrogenase From Desulfomicrobium Baculatum
Length = 498
Score = 98.7 bits (242), Expect = 1e-19
Identities = 118/495 (23%), Positives = 196/495 (38%), Gaps = 84/495 (16%)
Query: 6 IPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRF--FEAITIGKKLEEALAIYPR 63
+ I++D + RVEG +E+ V D V + K + G F FE I G+ ++ I R
Sbjct: 12 VKISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQR 68
Query: 64 ICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGY 123
IC C AH ++ A + A G + R +++ + ++SH LH Y L DY+
Sbjct: 69 ICGVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKG 128
Query: 124 SSPLKMV---------------------------NEYKKELEIALKLKNLGSWMMDVLGS 156
V N+Y K LEI + + M+ + G
Sbjct: 129 PDVSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEI----RRICHEMVAMFGG 184
Query: 157 RAIHQENAILGGFGKLPSKETLEEMKAKLRE--SLSLAEYTFELFAKLEQYREV------ 208
R H + ++GG ++P+ + + E A+ +E + EY ++ Y ++
Sbjct: 185 RMPHVQGMVVGGATEIPTADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIG 244
Query: 209 EGEITHLAVKPRGDVYGIY----GDYIKASDGEEFPSEDYKEHINEFVVEH--------- 255
+ V P D Y + G YI D EEF S+ KE++ +H
Sbjct: 245 WKNVIAFGVFPEDDDYKTFLLKPGVYIDGKD-EEFDSKLVKEYVGHSFFDHSAPGGLHYS 303
Query: 256 --------------SFAKHSHYKGKPFMVGAISRV-VNNKDLLYGRAKDLYESHKELLKG 300
SF K YK KP VG ++R+ V N +L K L E + K
Sbjct: 304 VGETNPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKK 363
Query: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERD------KVEVRDGF-GVSTTEA 353
+ + L + KW + + K E+ D G TEA
Sbjct: 364 FRDLGDKAFSIMGRHVLRAEETWLTAVAVEKWLKQVQPGAETYVKSEIPDAAEGTGFTEA 423
Query: 354 PRGILVYALKVENGRVAYADIITPTAFNLAMMEE-HVRMMAEKHYNDDPE---RLKLLAE 409
PRG L++ LK+++ ++ I++ T +N ++ R E+ P + +
Sbjct: 424 PRGALLHYLKIKDKKIENYQIVSATLWNANPRDDMGQRGPIEEALIGVPVPDIKNPVNVG 483
Query: 410 MVVRAYDPCISCSVH 424
+VR+YDP + C+VH
Sbjct: 484 RLVRSYDPXLGCAVH 498
>gb|AAA23375.2| (M18271) large hydrogenase subunit [Desulfomicrobium baculatum]
Length = 514
Score = 95.6 bits (234), Expect = 1e-18
Identities = 107/448 (23%), Positives = 177/448 (38%), Gaps = 80/448 (17%)
Query: 6 IPITVDHIARVEGKGGVEIIVGDEGVKEVKLNIIEGPRF--FEAITIGKKLEEALAIYPR 63
+ I++D + RVEG +E+ V D V + K + G F FE I G+ ++ I R
Sbjct: 13 VKISIDPLTRVEGHLKIEVEVKDGKVVDAKCS---GGMFRGFEQILRGRDPRDSSQIVQR 69
Query: 64 ICSFCSAAHKLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLGY 123
IC C AH ++ A + A G + R +++ + ++SH LH Y L DY+
Sbjct: 70 ICGVCPTAHCTASVMAQDDAFGVKVTTNGRITRNLIFGANYLQSHILHFYHLAALDYVKG 129
Query: 124 SSPLKMV---------------------------NEYKKELEIALKLKNLGSWMMDVLGS 156
V N+Y K LEI + + M+ + G
Sbjct: 130 PDVSPFVPRYANADLLTDRIKDGAKADATNTYGLNQYLKALEI----RRICHEMVAMFGG 185
Query: 157 RAIHQENAILGGFGKLPSKETLEEMKAKLRE--SLSLAEYTFELFAKLEQYREV------ 208
R H + ++GG ++P+ + + E A+ +E + EY ++ Y ++
Sbjct: 186 RMPHVQGMVVGGATEIPTADKVAEYAARFKEVQKFVIEEYLPLIYTLGSVYTDLFETGIG 245
Query: 209 EGEITHLAVKPRGDVYGIY----GDYIKASDGEEFPSEDYKEHINEFVVEH--------- 255
+ V P D Y + G YI D EEF S+ KE++ +H
Sbjct: 246 WKNVIAFGVFPEDDDYKTFLLKPGVYIDGKD-EEFDSKLVKEYVGHSFFDHSAPGGLHYS 304
Query: 256 --------------SFAKHSHYKGKPFMVGAISRV-VNNKDLLYGRAKDLYESHKELLKG 300
SF K YK KP VG ++R+ V N +L K L E + K
Sbjct: 305 VGETNPNPDKPGAYSFVKAPRYKDKPCEVGPLARMWVQNPELSPVGQKLLKELYGIEAKK 364
Query: 301 TNPFANNLAQALELVYFIERAIDLIDEVLIKWPVKERD------KVEVRDGF-GVSTTEA 353
+ + + L + KW + + K E+ D G TEA
Sbjct: 365 FRDLGDKAFSIMGRHVLVAEETWLTAVAVEKWLKQVQPGAETYVKSEIPDAAEGTGFTEA 424
Query: 354 PRGILVYALKVENGRVAYADIITPTAFN 381
PRG L++ LK+++ ++ I++ T +N
Sbjct: 425 PRGALLHYLKIKDKKIENYQIVSATLWN 452
>pir||F30315 methyl viologen-reducing hydrogenase (EC 1.-.-.-) alpha chain -
Methanobacterium thermoautotrophicum (strain winter)
(fragment)
Length = 242
Score = 95.2 bits (233), Expect = 1e-18
Identities = 61/202 (30%), Positives = 106/202 (52%), Gaps = 10/202 (4%)
Query: 6 IPITVDHIARVEGKGGVEIIVGDEG-VKEVKLNIIEGPRFFEAITIGKKLEEALAIYPRI 64
+ +T++ + R+EG + + + D G V++ +L+++E R FE G+ +EEA I PRI
Sbjct: 2 VKLTMEPVTRIEGHAKITVHLDDAGNVEDTRLHVMEF-RGFEKFLQGRPIEEAPRIVPRI 60
Query: 65 CSFCSAAHKLTALEAAEKAIGFTPREEIQA---LREVLYIGDMIESHALHLYLLVLPDYL 121
C C H L A +A + GF P + + A +RE++ G + SH LH Y L PD++
Sbjct: 61 CGICDVQHHLAAAKAVDACFGFEPDDILPAAYKMREIMNWGSYMHSHGLHFYFLAAPDFI 120
Query: 122 -GYSSPLKMVNEYKKEL-EIALKLKNLGSWMMDVL---GSRAIHQENAILGGFGKLPSKE 176
G + V + K+ ++AL+ L ++++ GSR IH + G E
Sbjct: 121 AGKDRKTRNVFQIIKDAPDVALQAIELRKNALELVRATGSRPIHPSFSTPRGISTELDDE 180
Query: 177 TLEEMKAKLRESLSLAEYTFEL 198
T +++ K++ ++ L E T EL
Sbjct: 181 TQKDLLKKVQRNVELVEATLEL 202
>gb|AAB49361.1| (U82565) [NiFe] hydrogenase-like protein large subunit [Alcaligenes
hydrogenophilus]
Length = 485
Score = 69.1 bits (166), Expect = 9e-11
Identities = 56/208 (26%), Positives = 90/208 (42%), Gaps = 15/208 (7%)
Query: 15 RVEGKGGVEIIVGDEGVKEVKLNIIEGPRF--FEAITIGKKLEEALAIYPRICSFCSAAH 72
RVEG V++ + D V+ K+N P F FE I +GK +AL PRIC CS +
Sbjct: 11 RVEGDLEVQLDIADGQVRTAKVN---APMFRGFEQILLGKLPMDALVYAPRICGICSVSQ 67
Query: 73 KLTALEAAEKAIGFTPREEIQALREVLYIGDMIESHALHLYLLVLPDYLG--YSS----- 125
+ A A E A G Q L ++ + + H H YL +PD+ Y+S
Sbjct: 68 SVAAARALEDATGVIAPLNGQLLTNLMLATENLADHLTHFYLFFMPDFARAIYASWAWHG 127
Query: 126 --PLKMVNEYKKELEIALKLKNLGSWMMDVLGSRAIHQENAILGGFGKLPSKETLEEMKA 183
+ + + ++AL + ++ +LG + H ++ GG + +
Sbjct: 128 TVHERFAAQQGRHAKLALAARQRWFTLLGMLGGKWPHTQSIQPGGSSRAVDASERIRLLV 187
Query: 184 KLRESLSLAEYTFELFAKLEQYREVEGE 211
K+RE S E T A LE+ ++ E
Sbjct: 188 KVREFRSFLEQTL-FGAPLEEVAALDSE 214
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.138 0.394
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 160257552
Number of Sequences: 2977
Number of extensions: 6852645
Number of successful extensions: 17454
Number of sequences better than 1.0e-10: 35
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 17328
Number of HSP's gapped (non-prelim): 42
length of query: 428
length of database: 189,106,746
effective HSP length: 57
effective length of query: 371
effective length of database: 154,992,987
effective search space: 57502398177
effective search space used: 57502398177
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)