BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1791 (mdh) DE:MALATE DEHYDROGENASE
(362 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||H75131 malate dehydrogenase PAB1791 - Pyrococcus abyssi (st... 731 0.0
sp|O59028|MDH_PYRHO MALATE DEHYDROGENASE >gi|7449808|pir||B71073... 657 0.0
sp|Q58820|MDH1_METJA MALATE DEHYDROGENASE I >gi|2129100|pir||H64... 260 2e-68
pir||S08689 malate dehydrogenase (EC 1.1.1.37) - Methanothermus ... 260 2e-68
sp|P16142|MDH_METFE MALATE DEHYDROGENASE >gi|99123|pir||S08981 m... 260 2e-68
pir||H69027 malate dehydrogenase - Methanobacterium thermoautotr... 240 3e-62
gb|AAF51687.1| (AE003593) CG10512 gene product [Drosophila melan... 236 2e-61
pir||T20396 hypothetical protein F36A2.3 - Caenorhabditis elegan... 236 2e-61
pir||E69852 malate dehydrogenase homolog yjmC - Bacillus subtili... 232 4e-60
pir||T18570 probable malate dehydrogenase (EC 1.1.1.37) - Caenor... 232 4e-60
gi|10956557 hypothetical protein [Lactobacillus helveticus] >gi|... 219 6e-56
sp|P77555|ALLD_ECOLI UREIDOGLYCOLATE DEHYDROGENASE >gi|7449805|p... 185 7e-46
sp|P30178|YBIC_ECOLI HYPOTHETICAL 38.9 KDA PROTEIN IN DING-GLNQ ... 177 2e-43
emb|CAB45039.1| (AL078635) putative carboxylic acid dehydrogenas... 177 2e-43
gb|AAA53657.1| (L02123) YbiC [Escherichia coli] 174 2e-42
gb|AAG22250.1| (AE003482) CG18808 gene product [Drosophila melan... 171 1e-41
sp|Q07251|LDH_ALCEU L-LACTATE DEHYDROGENASE >gi|2120947|pir||I39... 152 6e-36
gb|AAF55744.1| (AE003729) CG17199 gene product [Drosophila melan... 152 8e-36
sp|P44995|YIAK_HAEIN HYPOTHETICAL PROTEIN HI1031 >gi|1074616|pir... 140 3e-32
pir||S47796 hypothetical protein o332 - Escherichia coli >gi|466... 137 2e-31
sp|P37672|YIAK_ECOLI HYPOTHETICAL 36.6 KDA PROTEIN IN AVTA-SELB ... 136 3e-31
pir||B83488 probable L-malate dehydrogenase PA1252 [imported] - ... 133 3e-30
pir||T20825 hypothetical protein F13D12.1 - Caenorhabditis elegans 89 1e-16
>pir||H75131 malate dehydrogenase PAB1791 - Pyrococcus abyssi (strain Orsay)
>gi|5458280|emb|CAB49769.1| (AJ248285) MALATE
DEHYDROGENASE [Pyrococcus abyssi]
Length = 362
Score = 731 bits (1867), Expect = 0.0
Identities = 362/362 (100%), Positives = 362/362 (100%)
Query: 1 MFEKGYVDENYVRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRL 60
MFEKGYVDENYVRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRL
Sbjct: 1 MFEKGYVDENYVRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRL 60
Query: 61 KRYVDGILSGGINLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIV 120
KRYVDGILSGGINLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIV
Sbjct: 61 KRYVDGILSGGINLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIV 120
Query: 121 AVRNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKP 180
AVRNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKP
Sbjct: 121 AVRNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKP 180
Query: 181 FLLDMATSVVPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELL 240
FLLDMATSVVPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELL
Sbjct: 181 FLLDMATSVVPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELL 240
Query: 241 GGHKGYGLSLMVDILSGILSGGTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRM 300
GGHKGYGLSLMVDILSGILSGGTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRM
Sbjct: 241 GGHKGYGLSLMVDILSGILSGGTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRM 300
Query: 301 SEMINEIKNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGVKGL 360
SEMINEIKNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGVKGL
Sbjct: 301 SEMINEIKNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGVKGL 360
Query: 361 EV 362
EV
Sbjct: 361 EV 362
>sp|O59028|MDH_PYRHO MALATE DEHYDROGENASE >gi|7449808|pir||B71073 probable malate
dehydrogenase - Pyrococcus horikoshii
>gi|3257697|dbj|BAA30380.1| (AP000005) 360aa long
hypothetical malate dehydrogenase [Pyrococcus
horikoshii]
Length = 360
Score = 657 bits (1676), Expect = 0.0
Identities = 312/360 (86%), Positives = 344/360 (94%)
Query: 1 MFEKGYVDENYVRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRL 60
MFEKGYVDENY+R+PKD LFSF+VRVLTKLG PEEDAKIVADNL+MADLRG+ESHGVQRL
Sbjct: 1 MFEKGYVDENYIRVPKDRLFSFIVRVLTKLGVPEEDAKIVADNLVMADLRGVESHGVQRL 60
Query: 61 KRYVDGILSGGINLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIV 120
KRYVDGI+SGG+NLHP+I++IREGPSYAL+DGDEG GQVVGY++MKLAI+KA+ TGIGIV
Sbjct: 61 KRYVDGIISGGVNLHPKIRVIREGPSYALIDGDEGLGQVVGYRSMKLAIKKAKDTGIGIV 120
Query: 121 AVRNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKP 180
RNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGG+ERILGTNPIALAAPTK KP
Sbjct: 121 IARNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGIERILGTNPIALAAPTKDKP 180
Query: 181 FLLDMATSVVPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELL 240
FLLDMATSVVPIGKLEVYRRKG++IPEGWAIN +G IT VEEVFNGG+LLPLGGFGELL
Sbjct: 181 FLLDMATSVVPIGKLEVYRRKGKDIPEGWAINREGNITTKVEEVFNGGALLPLGGFGELL 240
Query: 241 GGHKGYGLSLMVDILSGILSGGTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRM 300
GGHKGYGLSLMVDILSGILSGGTWSK+VKNT+EK SNVCHFFM I+IEHF PLEEFK ++
Sbjct: 241 GGHKGYGLSLMVDILSGILSGGTWSKYVKNTSEKGSNVCHFFMVIDIEHFIPLEEFKEKI 300
Query: 301 SEMINEIKNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGVKGL 360
S+MI EIK+SRKHP+FERIWIHGEKGFLTMETRLKLGIPIY+KVL+ELNEIA+RVGV+GL
Sbjct: 301 SQMIEEIKSSRKHPEFERIWIHGEKGFLTMETRLKLGIPIYRKVLEELNEIAKRVGVEGL 360
>sp|Q58820|MDH1_METJA MALATE DEHYDROGENASE I >gi|2129100|pir||H64477 malate dehydrogenase
(EC 1.1.1.37) - Methanococcus jannaschii
>gi|1592075|gb|AAB99436.1| (U67583) malate dehydrogenase
[Methanococcus jannaschii]
Length = 344
Score = 260 bits (657), Expect = 2e-68
Identities = 153/341 (44%), Positives = 209/341 (60%), Gaps = 21/341 (6%)
Query: 23 VVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGINLHPRIKIIR 82
++ VL K G PEEDAKI AD + ADL+G SHG+ R +Y+ + G IN P IKI++
Sbjct: 12 IIDVLKKFGVPEEDAKITADVFVDADLKGFTSHGIGRFPQYITALKLGNINPKPDIKIVK 71
Query: 83 EGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGYYALMAAEEG 142
E P+ A++DGD G GQVVG KAM+LAI+KA+ G+G+VA RN+NH+GIAGYY+ +A +
Sbjct: 72 ESPATAVIDGDLGLGQVVGKKAMELAIKKAKNVGVGVVATRNANHFGIAGYYSELAMNQD 131
Query: 143 MIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVPIGKLEVYRRKG 202
MIGI++TN+ P +AP GG E+ILGTNPIA+A F LDMAT+ + GK+ RK
Sbjct: 132 MIGITITNTEPAMAPFGGKEKILGTNPIAIAFKGNKYKFSLDMATASIARGKILEALRKK 191
Query: 203 EEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVDILSGILSGG 262
+IPEG A++ G+ T + G +LP GG KGYGL+L +++LS I G
Sbjct: 192 IKIPEGCAVDKDGKPTTDPAKALE-GCILP-------FGGPKGYGLALAIEMLSAI-GGA 242
Query: 263 TWSKHVKNT--NEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSRKHPDFERIW 320
VK T E+ F+AIN E F EEFK ++ E+++EIKNS FE I
Sbjct: 243 EVGTKVKGTANPEERCTKGDLFIAINPEFFMGKEEFKRKVDELLDEIKNSEPAEGFE-IL 301
Query: 321 IHGEKGFLTMETRLKL----GIPIYKKVLDELNEIARRVGV 357
I GE +E R K+ G I K + ++L EI +G+
Sbjct: 302 IPGE-----IEERNKMKRKDGFEIDKNLYNQLKEICNELGL 337
>pir||S08689 malate dehydrogenase (EC 1.1.1.37) - Methanothermus fervidus
(fragment) >gi|44278|emb|CAA36133.1| (X51840) L-malate
dehydrogenase (AA 339) [Methanothermus fervidus]
Length = 339
Score = 260 bits (657), Expect = 2e-68
Identities = 149/348 (42%), Positives = 212/348 (60%), Gaps = 17/348 (4%)
Query: 14 IPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGIN 73
I +E S ++++L LG EE AKI AD ++ ADL+G SHG+ R +YV+GI G I
Sbjct: 3 ISPEEERSLIIKILNALGVSEEHAKITADVIVDADLKGFTSHGIGRFPQYVEGIKLGTIK 62
Query: 74 LHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGY 133
I+I +E S AL++G+ GQVV YK MKLAIEKA+ TG+GIV + +SNH+GIAGY
Sbjct: 63 TSGNIEIEKETDSVALINGNHLLGQVVAYKGMKLAIEKAKNTGVGIVGIHDSNHFGIAGY 122
Query: 134 YALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVPIG 193
Y+ MA + MIGI+MTN+ P VAP GG +LGTNPIA++ P+ +DM+T+ V G
Sbjct: 123 YSDMAMKNDMIGITMTNTEPAVAPLGGKIPVLGTNPIAISIPSNEYYVAVDMSTAAVARG 182
Query: 194 KLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVD 253
KL RK E+IPEG A++ G T E N GS+LP GGHKGY L M++
Sbjct: 183 KLLEAARKNEKIPEGIAVDKNGNPTTDPNEALN-GSILP-------FGGHKGYALCFMIE 234
Query: 254 ILSGILSGGTWSKHVKNTNEKNSNVC---HFFMAINIEHFTPLEEFKGRMSEMINEIKNS 310
IL+G L + VK T + S +C +AI+ F +EEFK + E + EIK++
Sbjct: 235 ILAGPLVKAEFGSKVKGTVDP-SQMCTKGDLLIAIDPSKFYDIEEFKRNVDEFVKEIKST 293
Query: 311 RKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGVK 358
K + I G++ + ++ R K GI + KK++++L EIA + ++
Sbjct: 294 GKD-----VLIPGDRERMNIKKREKEGIELDKKLVEKLKEIADELNIE 336
>sp|P16142|MDH_METFE MALATE DEHYDROGENASE >gi|99123|pir||S08981 malate dehydrogenase (EC
1.1.1.37) - Methanothermus fervidus
>gi|809713|emb|CAA36010.1| (X51714) L-malate
dehydrogenase (AA 1-339) [Methanothermus fervidus]
Length = 339
Score = 260 bits (657), Expect = 2e-68
Identities = 149/348 (42%), Positives = 212/348 (60%), Gaps = 17/348 (4%)
Query: 14 IPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGIN 73
I +E S ++++L LG EE AKI AD ++ ADL+G SHG+ R +YV+GI G I
Sbjct: 3 ISPEEERSLIIKILNALGVSEEHAKITADVIVDADLKGFTSHGIGRFPQYVEGIKLGTIK 62
Query: 74 LHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGY 133
I+I +E S AL++G+ GQVV YK MKLAIEKA+ TG+GIV + +SNH+GIAGY
Sbjct: 63 TSGNIEIEKETDSVALINGNHLLGQVVAYKGMKLAIEKAKNTGVGIVGIHDSNHFGIAGY 122
Query: 134 YALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVPIG 193
Y+ MA + MIGI+MTN+ P VAP GG +LGTNPIA++ P+ +DM+T+ V G
Sbjct: 123 YSDMAMKNDMIGITMTNTEPAVAPLGGKIPVLGTNPIAISIPSNEYYVAVDMSTAAVARG 182
Query: 194 KLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVD 253
KL RK E+IPEG A++ G T E N GS+LP GGHKGY L M++
Sbjct: 183 KLLEAARKNEKIPEGIAVDKNGNPTTDPNEALN-GSILP-------FGGHKGYALCFMIE 234
Query: 254 ILSGILSGGTWSKHVKNTNEKNSNVC---HFFMAINIEHFTPLEEFKGRMSEMINEIKNS 310
IL+G L + VK T + S +C +AI+ F +EEFK + E + EIK++
Sbjct: 235 ILAGPLVKAEFGSKVKGTVDP-SQMCTKGDLLIAIDPSKFYDIEEFKRNVDEFVKEIKST 293
Query: 311 RKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGVK 358
K + I G++ + ++ R K GI + KK++++L EIA + ++
Sbjct: 294 GKD-----VLIPGDRERMNIKKREKEGIELDKKLVEKLKEIADELNIE 336
>pir||H69027 malate dehydrogenase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622314|gb|AAB85694.1| (AE000888)
malate dehydrogenase [Methanobacterium
thermoautotrophicum]
Length = 341
Score = 240 bits (605), Expect = 3e-62
Identities = 141/348 (40%), Positives = 201/348 (57%), Gaps = 16/348 (4%)
Query: 12 VRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGG 71
+RI +E + +LT + PEE + IVAD + ADL+G SHG+ R +YVDG+ G
Sbjct: 1 MRISPEEEVKIIKEILTAMNVPEESSDIVADVTLDADLKGFSSHGIGRFPQYVDGLRHGT 60
Query: 72 INLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIA 131
I I I RE S AL++G+ FG VV Y+AM+LAIEKAR TG+G+V V +SNH+G+A
Sbjct: 61 IRADGDITIERETESTALINGNHIFGHVVAYRAMELAIEKARNTGVGLVGVHDSNHFGVA 120
Query: 132 GYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVP 191
GYY+ MA MIG+ + N+ P VAP GG + ILGTNP+A+ P+ +DMATS
Sbjct: 121 GYYSDMAVMNDMIGVVIANTEPAVAPIGGRKPILGTNPVAIGIPSNRYYVSVDMATSASA 180
Query: 192 IGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLM 251
GKL RKGE IPE A++++G+ T E GS+LP GGHKGY LS M
Sbjct: 181 RGKLLEAARKGESIPENVALDAEGKPTTDPEMALK-GSILP-------FGGHKGYALSFM 232
Query: 252 VDILSGILSGGTWSKHVKNT--NEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKN 309
++IL+G L G + V T E+ MAI+ +EF+ ++ E I E+K+
Sbjct: 233 IEILAGPLVGAAFGTAVTGTANPEEMCTKGDLMMAIDPSKMVDPDEFRAQVDEFIEEVKS 292
Query: 310 SRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGV 357
S + I G+ + ++ R GI + +K+L+ + IAR + +
Sbjct: 293 SGD------VLIPGDIESMNIKRRRAEGIELDEKLLERILGIARELDI 334
>gb|AAF51687.1| (AE003593) CG10512 gene product [Drosophila melanogaster]
Length = 446
Score = 236 bits (597), Expect = 2e-61
Identities = 126/309 (40%), Positives = 191/309 (61%), Gaps = 8/309 (2%)
Query: 22 FVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGINLHPRIKII 81
F++ + P+ A+ AD L+ AD RG SHG+ RL+ Y++ + + KI+
Sbjct: 102 FMIDCFKAVKVPQAHAEAQADLLVAADHRGHFSHGMNRLEMYINDLAINSTDGAAVPKIL 161
Query: 82 REGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGYYALMAAEE 141
+E P+ A +DG G G VVG M LAI+KA+ G+G V + SNHYG+AG+YA+ A ++
Sbjct: 162 KETPATAWVDGLNGLGAVVGNYCMDLAIKKAKTVGVGWVCAKGSNHYGMAGWYAIRAMDQ 221
Query: 142 GMIGISMTNSRPLVAPTGGVERILGTNPIALAA-PTKGKPFLLDMATSVVPIGKLEVYRR 200
G++G+SMTN+ PL+APT E LGTNP++L A T G FLLDMAT+ V +GK+E+ RR
Sbjct: 222 GLVGMSMTNTSPLMAPTRAKEAALGTNPLSLGANATNGDKFLLDMATTAVAVGKIEIQRR 281
Query: 201 KGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVDILSGILS 260
KG +P+GWA + GE+T E F+ G L+PLGG EL G+KGYGL MVDILSG++S
Sbjct: 282 KGAPLPDGWAQDPSGEVTNDAELGFSTGCLMPLGG-SELTSGYKGYGLGAMVDILSGVMS 340
Query: 261 GGTWSKHVKNTN----EKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSRKHPDF 316
G +S V+ + +++ F+A++ F P F+ RM++ + ++ +
Sbjct: 341 GANYSTQVRKWTHAGADSAADLGQVFIAVDPNCFAP--NFEERMADFNSRLRGATPTDPS 398
Query: 317 ERIWIHGEK 325
+ + + G+K
Sbjct: 399 KPVLLAGDK 407
>pir||T20396 hypothetical protein F36A2.3 - Caenorhabditis elegans
>gi|3875353|emb|CAB09417.1| (Z96047) cDNA EST
EMBL:M88984 comes from this gene~cDNA EST EMBL:T01848
comes from this gene~cDNA EST yk592h12.3 comes from this
gene~cDNA EST yk592h12.5 comes from this gene
[Caenorhabditis elegans] >gi|3876781|emb|CAB03073.1|
(Z81077) cDNA EST EMBL:M88984 comes from this gene~cDNA
EST EMBL:T01848 comes from this gene~cDNA EST yk592h12.3
comes from this gene~cDNA EST yk592h12.5 comes from this
gene [Caenorhabditis elegans]
Length = 372
Score = 236 bits (597), Expect = 2e-61
Identities = 140/360 (38%), Positives = 211/360 (57%), Gaps = 8/360 (2%)
Query: 3 EKGYVDENYVRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKR 62
++ + + + + I K++L SFV+ L K G + A+ +A+ L+ +D RG SHG+ RL
Sbjct: 6 KREFNETDEIVISKEKLDSFVLECLAKAGCTGDHAQQLAETLLCSDYRGHYSHGINRLHI 65
Query: 63 YVDGILSGGINLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAV 122
YV ++ + ++++ S A +DG+ G VVG M+LA+EKA++ GIG V
Sbjct: 66 YVHDLMMKSTAVTGTPQVLKSKGSTAWVDGNNLLGPVVGNFCMQLAVEKAKEFGIGWVVC 125
Query: 123 RNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAP-TKGKPF 181
RNSNH+GIAG+YA A G++G++ TN+ P V PTG E+ LG+NPI +AAP +G F
Sbjct: 126 RNSNHFGIAGWYADFACRNGLVGMAFTNTSPCVFPTGSREKSLGSNPICMAAPGMEGDSF 185
Query: 182 LLDMATSVVPIGKLEVYRRKGEE-IPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELL 240
LDMA++ V GK+EV RKGE IP W + G+ T + +EV +GG L PLGG E+
Sbjct: 186 FLDMASTTVAYGKIEVVDRKGETYIPGSWGADKNGDETHNPKEVLDGGGLQPLGG-SEIT 244
Query: 241 GGHKGYGLSLMVDILSGILSGGTWSKHVK--NTNEKNSNVCHFFMAINIEHFTPLEEFKG 298
GG+KG GL +MV++L GI+ G + K+++ T K +++ F+AI+ E F P F
Sbjct: 245 GGYKGTGLCMMVEVLCGIMGGSAFGKNIRQWQTTSKTADLGQCFVAIDPECFAP--GFSN 302
Query: 299 RMSEMINEIKNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIY-KKVLDELNEIARRVGV 357
R+ E +E +N + G+ M LG +Y KK LD L +A R+GV
Sbjct: 303 RLQEFCDETRNLNPINPSRPPQVPGDPERAHMNMCDDLGGIVYKKKQLDHLKNLADRLGV 362
>pir||E69852 malate dehydrogenase homolog yjmC - Bacillus subtilis
>gi|2612904|gb|AAC46328.1| (AF015825) malate
dehydrogenase-like protein [Bacillus subtilis]
>gi|2633586|emb|CAB13089.1| (Z99110) similar to malate
dehydrogenase [Bacillus subtilis]
Length = 337
Score = 232 bits (586), Expect = 4e-60
Identities = 136/341 (39%), Positives = 193/341 (55%), Gaps = 14/341 (4%)
Query: 12 VRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGG 71
+ I +E V + L G E DA+ VAD L+ ADLR + SHGV + YV+ +L+GG
Sbjct: 4 ITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLAGG 63
Query: 72 INLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIA 131
IN + GP +LDGD+GFG V AM AI+ A+K G+G+V NS+H G
Sbjct: 64 INPGAQPVFKETGPVTGVLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCGAL 123
Query: 132 GYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTK-GKPFLLDMATSVV 190
Y+ AA+E +IG++MT++ +V P GG ILGTNPIA P K KPF+LDMATS V
Sbjct: 124 SYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTNPIAYGVPAKHKKPFILDMATSKV 183
Query: 191 PIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSL 250
GK+ R +G+EIPEGW ++ GE ++V + + GG KGYGLS+
Sbjct: 184 AFGKILQAREEGKEIPEGWGVDENGEAVTDPDKVVSLST----------FGGPKGYGLSI 233
Query: 251 MVDILSGILSGGTWSKHVK---NTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEI 307
+VD+ SG+L+G + H+ N ++ + H+ AIN FT + F +M MI+E+
Sbjct: 234 VVDVFSGLLAGAAFGPHIAKMYNGLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDEL 293
Query: 308 KNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDEL 348
+ S FER+++ GE L E K GI I + V + L
Sbjct: 294 QQSPPAVGFERVYVPGEIEQLHEERNKKNGISIARSVYEFL 334
>pir||T18570 probable malate dehydrogenase (EC 1.1.1.37) - Caenorhabditis
elegans >gi|3947447|emb|CAA22133.1| (AL033535) predicted
using Genefinder~cDNA EST EMBL:M88999 comes from this
gene~cDNA EST yk268h6.3 comes from this gene~cDNA EST
yk264b4.3 comes from this gene~cDNA EST yk331d3.3 comes
from this gene~cDNA EST yk465f2.3 comes from this
gene~cDNA EST yk3> >gi|3947542|emb|CAA88951.1| (Z49127)
predicted using Genefinder~cDNA EST EMBL:M88999 comes
from this gene~cDNA EST yk268h6.3 comes from this
gene~cDNA EST yk264b4.3 comes from this gene~cDNA EST
yk331d3.3 comes from this gene~cDNA EST yk465f2.3 comes
from this gene~cDNA EST yk393>
Length = 400
Score = 232 bits (586), Expect = 4e-60
Identities = 140/358 (39%), Positives = 205/358 (57%), Gaps = 17/358 (4%)
Query: 14 IPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGIN 73
+ KDE+ F+V +TK+G E A +A L+ D+RG SHG+ RL YV I
Sbjct: 49 VAKDEMKRFMVECMTKVGATESHATQLALVLLEGDIRGHYSHGLNRLDMYVRDIEQNVCK 108
Query: 74 LHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGY 133
I++E A +DG+ G VVG M LAIEKA+ GIG V + SNHYGIAG+
Sbjct: 109 GDGEPIILKEKAGTAWVDGNNLLGPVVGNFCMDLAIEKAKNAGIGWVVAKGSNHYGIAGW 168
Query: 134 YALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKG-KPFLLDMATSVVPI 192
YAL A ++GM+G+SMTN+ P+ PT LGTNPI+LAAP G F+LDMA++ V I
Sbjct: 169 YALRAMKKGMLGMSMTNTSPISFPTRSAVPALGTNPISLAAPGTGDDSFVLDMASTTVAI 228
Query: 193 GKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMV 252
GK+E+ RK +P W + G+ T +V GG LLPLGG E+ GG+KGYGLS M+
Sbjct: 229 GKVELAARKENPVPLSWGVGEGGKETTDPTKVLYGGGLLPLGGV-EVSGGYKGYGLSSMI 287
Query: 253 DILSGILSGGTWSKHVKN--TNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNS 310
+I GIL+G W HV+ + + +++ F+AI+ E F P F R+ + + ++
Sbjct: 288 EIFCGILAGAHWGPHVRKWMSTKSEADLGQCFVAIDPEAFAP--GFADRLQDFMQTMRAL 345
Query: 311 RKHPDFERIWIHGEKGFLTMETRLKL------GIPIYKKVLDELNEIARRVGVKGLEV 362
++ + G+ ME R + GIP +K + +N++A ++GVK +++
Sbjct: 346 PTSSPSFKVEVAGD-----MERRHEALVEQLGGIPYHKNQITFVNDLAAKLGVKTVDL 398
>gi|10956557 hypothetical protein [Lactobacillus helveticus]
>gi|11280078|pir||T46704 hypothetical protein orf-361
[imported] - Lactobacillus helveticus plasmid pLH1
>gi|2687745|emb|CAA10971.1| (AJ222725) hypothetical
protein [Lactobacillus helveticus]
Length = 361
Score = 219 bits (551), Expect = 6e-56
Identities = 123/349 (35%), Positives = 194/349 (55%), Gaps = 17/349 (4%)
Query: 22 FVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGINLHPRIKII 81
F+ +V + D ++AD L+ ADLRGI SHG+QRL Y I I ++K+I
Sbjct: 11 FLEKVFAARQFSKSDGALLADTLVDADLRGISSHGIQRLAWYTSMIKEHIIEPQNKLKVI 70
Query: 82 REGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGYYALMAAEE 141
+E P+ L+D ++ GQ+ A K I+K +K G+ + +RNSNH+G AGYYA MAA++
Sbjct: 71 KETPTSLLIDANKNMGQIATAFAEKQLIKKTQKLGLSVAVIRNSNHFGTAGYYARMAAKQ 130
Query: 142 GMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVPIGKLEVYRRK 201
G+IGI++TN+RPLV P E LG+N A P + PF+ D ATS+V GK+++ +
Sbjct: 131 GLIGIALTNTRPLVVPPNATEAFLGSNAFAFPLPAEPHPFVFDGATSIVSSGKIQLLAKN 190
Query: 202 GEEIPEGWAINSKGEITRSVEEVFN------------GGSLLPLGGFGELLGGHKGYGLS 249
+ IP WA+++ + ++V N GG +L LGG + +KG+G S
Sbjct: 191 NQPIPGDWAVDNHRRVMHDAQKVENNLAKVAFTEKQPGGGVLTLGGLLQSNSNYKGFGNS 250
Query: 250 LMVDILSGILSGGTWSKHVKNTNEK-NSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIK 308
L+V++L+GIL+ G+ S +TN+ + + F + IN E F L+ K EM + ++
Sbjct: 251 LVVELLTGILAQGSIS---ADTNKPGHHDFSQFLLTINPELFGNLDNLKNSAEEMFDRLR 307
Query: 309 NSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGV 357
+ + P E I I G++ + LK G+ I K + EL I + +
Sbjct: 308 HLKHLPGAE-IMIPGDREYRRYAENLKQGVVIDDKTVAELKTIGEELAI 355
>sp|P77555|ALLD_ECOLI UREIDOGLYCOLATE DEHYDROGENASE >gi|7449805|pir||D64783 malate
dehydrogenase homolog ylbC - Escherichia coli
>gi|1773197|gb|AAB40269.1| (U82664) similar to M.
fervidus malate dehydrogenase [Escherichia coli]
>gi|1786727|gb|AAC73619.1| (AE000157) putative malate
dehydrogenase (EC 1.1.1.37) [Escherichia coli K12]
>gi|2735243|gb|AAB93858.1| (U89279) GlxB8 [Escherichia
coli]
Length = 349
Score = 185 bits (465), Expect = 7e-46
Identities = 113/341 (33%), Positives = 168/341 (49%), Gaps = 14/341 (4%)
Query: 12 VRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGG 71
++I ++ L + L + G E A VA+ L+ AD RGI SHG R++ Y + I GG
Sbjct: 1 MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSHGAVRVEYYAERISKGG 60
Query: 72 INLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIA 131
N P ++ GP A+L D GQV M+ AI+ A++ G+ +V + H G
Sbjct: 61 TNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQQNGVAVVGISRMGHSGAI 120
Query: 132 GYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFL-LDMATSVV 190
Y+ AA G IGISM S P+V P GG E GTNP+A AAP +G L DMAT+V
Sbjct: 121 SYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLAFAAPGEGDEILTFDMATTVQ 180
Query: 191 PIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSL 250
GK+ R + IP+ WA++ G T + F +LLP G KGYGL +
Sbjct: 181 AWGKVLDARSRNMSIPDTWAVDKNGVPT---TDPFAVHALLP-------AAGPKGYGLMM 230
Query: 251 MVDILSGILSGGTWSKHVKNTNE---KNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEI 307
M+D+LSG+L G + + V + + N+ + IN F+ E F+ +S+ + E+
Sbjct: 231 MIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSSELFRQHLSQTMREL 290
Query: 308 KNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDEL 348
P F +++ G+ + GI I + L
Sbjct: 291 NAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYL 331
>sp|P30178|YBIC_ECOLI HYPOTHETICAL 38.9 KDA PROTEIN IN DING-GLNQ INTERGENIC REGION (O361)
>gi|7449094|pir||A64817 malate dehydrogenase homolog
ybiC - Escherichia coli >gi|1787020|gb|AAC73888.1|
(AE000182) putative dehydrogenase [Escherichia coli K12]
>gi|4062362|dbj|BAA35467.1| (D90717) Hypothetical 38.9
kd protein in ding/rarB 3'region (o361). [Escherichia
coli]
Length = 361
Score = 177 bits (444), Expect = 2e-43
Identities = 123/349 (35%), Positives = 172/349 (49%), Gaps = 11/349 (3%)
Query: 13 RIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGI 72
R L SF+ V ++G+ E++AK+VAD+LI A+L G +SHG+ + YV G +
Sbjct: 6 RFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHL 65
Query: 73 NLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAG 132
++ K ++E + LDGD FGQV ++AM L IEKA + GI VA+ NS+H G G
Sbjct: 66 QINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIG 125
Query: 133 YYALMAAEEGMIGISMTN--SRPLVAPTGGVERILGTNPIALAAPTKGK-PFLLDMATSV 189
Y+A A G + I + P+VAP G + GTNP + P K P LLD ATS
Sbjct: 126 YWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRFGTNPFCVVFPRKDNFPLLLDYATSA 185
Query: 190 VPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLS 249
+ GK V KG +P G I+ G T + V L L F E HKGY L+
Sbjct: 186 IAFGKTRVAWHKGVPVPPGCLIDVNGVPTTN-PAVMQESPLGSLLTFAE----HKGYALA 240
Query: 250 LMVDILSGILSGG-TWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIK 308
M +IL G LSGG T + T+ C + IN E F + +E E
Sbjct: 241 AMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPD--CNAQTEAFAEWV 298
Query: 309 NSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGV 357
+ H D + I + GE T R K GIP+ + + AR++G+
Sbjct: 299 KASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGSWQAICDAARQIGM 347
>emb|CAB45039.1| (AL078635) putative carboxylic acid dehydrogenase [Amycolatopsis
orientalis]
Length = 337
Score = 177 bits (444), Expect = 2e-43
Identities = 114/339 (33%), Positives = 174/339 (50%), Gaps = 15/339 (4%)
Query: 18 ELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGINLHPR 77
EL + R+L G+ DA+ VA +L+ +DL G +SHGV+R+ YV I +G I+
Sbjct: 7 ELEALATRILVAAGSSTVDAETVARSLVGSDLLGHDSHGVRRVIDYVKRIHTGLIDPRAE 66
Query: 78 IKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGYYALM 137
K P ++ G FGQ+ +A++ + G G+ VR NH G G Y M
Sbjct: 67 PKAETTRPGTVVVHGRRAFGQLAVRRAVRELLSLVPHQGSGVAVVRECNHIGRLGEYVAM 126
Query: 138 AAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAP--TKGKPFLLDMATSVVPIGKL 195
A++ +IG++ N+ P VAP GG ER LGTNP+A A P P ++D ATS + GKL
Sbjct: 127 LADQDLIGLAFGNADPTVAPYGGRERRLGTNPLAWAVPRGPGQPPIVMDWATSGIAEGKL 186
Query: 196 EVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVDIL 255
V R +GE++ EG +++ G + + + + GG+LLP GGHKGYGLS++++I+
Sbjct: 187 RVARDRGEQVAEGLVLDAAGRPSTTPADFYQGGALLP-------FGGHKGYGLSVLIEIV 239
Query: 256 SGILSG-GTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSRKHP 314
G+LSG G S + E V +A +I F P+ EF+ + + +
Sbjct: 240 GGLLSGIGIGS--MPGFREGWGTV---LVAFDIAAFLPVPEFRAQTERFCQALAETPLAE 294
Query: 315 DFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIAR 353
+ + GE R + GIP+ ELN + R
Sbjct: 295 GASEVLVPGEPEERIRLERARDGIPVTANTWRELNALLR 333
>gb|AAA53657.1| (L02123) YbiC [Escherichia coli]
Length = 361
Score = 174 bits (436), Expect = 2e-42
Identities = 122/349 (34%), Positives = 171/349 (48%), Gaps = 11/349 (3%)
Query: 13 RIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGI 72
R L SF+ V ++ + E++AK+VAD+LI A+L G +SHG+ + YV G +
Sbjct: 6 RFDAQTLHSFIQAVFRQIPSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHL 65
Query: 73 NLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAG 132
++ K ++E + LDGD FGQV ++AM L IEKA + GI VA+ NS+H G G
Sbjct: 66 QINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIG 125
Query: 133 YYALMAAEEGMIGISMTN--SRPLVAPTGGVERILGTNPIALAAPTKGK-PFLLDMATSV 189
Y+A A G + I + P+VAP G + GTNP + P K P LLD ATS
Sbjct: 126 YWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRFGTNPFCVVFPRKDNFPLLLDYATSA 185
Query: 190 VPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLS 249
+ GK V KG +P G I+ G T + V L L F E HKGY L+
Sbjct: 186 IAFGKTRVAWHKGVPVPPGCLIDVNGVPTTN-PAVMQESPLGSLLTFAE----HKGYALA 240
Query: 250 LMVDILSGILSGG-TWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIK 308
M +IL G LSGG T + T+ C + IN E F + +E E
Sbjct: 241 AMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPD--CNAQTEAFAEWV 298
Query: 309 NSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEIARRVGV 357
+ H D + I + GE T R K GIP+ + + AR++G+
Sbjct: 299 KASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGSWQAICDAARQIGM 347
>gb|AAG22250.1| (AE003482) CG18808 gene product [Drosophila melanogaster]
Length = 385
Score = 171 bits (428), Expect = 1e-41
Identities = 114/347 (32%), Positives = 169/347 (47%), Gaps = 10/347 (2%)
Query: 17 DELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGINLHP 76
DE F+ L ++G + +++ L++AD RG G+ RL Y+ + SG +
Sbjct: 37 DEARRFIQDCLLRVGVSPSKVRCISEFLVVADYRGNYGSGLNRLDYYLSDLQSGHAKVGA 96
Query: 77 RIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGYYAL 136
II E S A ++G+ G VG M LA++KA +GIG V + S+ G+A ++
Sbjct: 97 EPSIISETVSTAHVNGNSALGVFVGNFCMDLAVKKAEDSGIGFVVAQQSHDIGMASWFTF 156
Query: 137 MAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVPIGKLE 196
AA +G+ GI M+NS P + +G+N A + F+LDMATSV IG +E
Sbjct: 157 RAAGKGLAGIVMSNSAPTMMGPNSKSASIGSNCFAFCVKGEEYHFVLDMATSVKDIGAVE 216
Query: 197 VYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVDILS 256
E IP GWA N G T L P GGHKGY LS ++DIL
Sbjct: 217 WAWANDEYIPHGWAANEGGLSTCFPSLALRTPLLFP-------AGGHKGYCLSAVIDILC 269
Query: 257 GILSGGTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSRKHPDF 316
G+LSG ++ H+ + SN+ F+A++ E F P F R + I+NS+ D
Sbjct: 270 GVLSGAQYATHITMDQNQPSNLGQVFIALDPEFFLP--NFMERFDDFCGRIQNSQPADDS 327
Query: 317 ERIWIHGEKGFLTMETRLKLGIPIY-KKVLDELNEIARRVGVKGLEV 362
E I + GE + M L Y +L + E+A R+ VK +E+
Sbjct: 328 EPIRLPGELERMHMNYVEDLRALAYPNSLLTKYKEVAERLCVKPIEL 374
>sp|Q07251|LDH_ALCEU L-LACTATE DEHYDROGENASE >gi|2120947|pir||I39535 probable L-lactate
dehydrogenase (EC 1.1.1.27) - Alcaligenes eutrophus
>gi|311310|emb|CAA80432.1| (Z22737) lactate
dehydrogenase [Ralstonia eutropha]
Length = 349
Score = 152 bits (380), Expect = 6e-36
Identities = 93/244 (38%), Positives = 130/244 (53%), Gaps = 14/244 (5%)
Query: 26 VLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGINLHPRIKIIREGP 85
+L P + A VA++L+ +D G SHG+ L Y + +N R K + +
Sbjct: 15 ILAAQQVPADIADDVAEHLVESDRCGYISHGLSILPNYRTALDGHSVNPQGRAKCVLDQG 74
Query: 86 SYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGYYALMAAEEGMIG 145
+ + DGD GFGQ VG M+ AIE+ R+ G IV +R S+H G G+Y MAA G +
Sbjct: 75 TLMVFDGDGGFGQHVGKSVMQAAIERVRQHGHCIVTLRRSHHLGRMGHYGEMAAAAGFVL 134
Query: 146 ISMT---NSRPLVAPTGGVERILGTNPIALAAPTKG--KPFLLDMATSVVPIGKLEVYRR 200
+S T N P+VAP GG L TNP+ A P P ++D+ATS + I K V
Sbjct: 135 LSFTNVINRAPVVAPFGGRVARLTTNPLCFAGPMPNGRPPLVVDIATSAIAINKARVLAE 194
Query: 201 KGEEIPEGWAINSKGEITRSVEEVF--NGGSLLPLGGFGELLGGHKGYGLSLMVDILSGI 258
KGE PEG I + G T +F + G+LLP GGHKGY L ++ ++L+G+
Sbjct: 195 KGEPAPEGSIIGADGNPTTDASTMFGEHPGALLP-------FGGHKGYALGVVAELLAGV 247
Query: 259 LSGG 262
LSGG
Sbjct: 248 LSGG 251
>gb|AAF55744.1| (AE003729) CG17199 gene product [Drosophila melanogaster]
Length = 461
Score = 152 bits (379), Expect = 8e-36
Identities = 105/349 (30%), Positives = 177/349 (50%), Gaps = 16/349 (4%)
Query: 22 FVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGGINLHPRIKII 81
FV +V + + P++ A +AD LI AD G S G+ RL +L+ + I+
Sbjct: 104 FVSQVFSAMQVPKQAASEMADALIAADYMGQRSMGIHRLPSIAADLLNCTVAGDATPGIV 163
Query: 82 REGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIAGYYALMAAEE 141
E + AL+DG G VV M LA++KAR+ GIG V+ R+SN G A +YA A E+
Sbjct: 164 SEKKAIALVDGHNAPGPVVANFCMDLALQKAREVGIGWVSARSSNCIGFASWYACQALEQ 223
Query: 142 GMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVPIGKLEVYRRK 201
MIG+ MTN+ P + P GG+E +LG NPIA AA + F+ D + + +LE+
Sbjct: 224 RMIGLCMTNAAPTLLPAGGIEPVLGENPIACAASGVHEQFVADFGMAACSVDELELSYCN 283
Query: 202 G--EEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVDILSGIL 259
G +E+P+ A++ G+ T S EE + HKG+GL+ VDIL G++
Sbjct: 284 GWSKEVPKLVALDRNGKETTSTEEALRAQRI------RAFQPEHKGFGLAAAVDILCGVM 337
Query: 260 SGGTWSKHVK-----NTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSRKHP 314
+G ++ ++ +T +N+ ++AI+ F P F+ R+++ ++ +
Sbjct: 338 TGARYANQIQRRGVYSTENAPANLGQVYVAIDPMRFCP--TFEDRLADFHRLLRQAVPSK 395
Query: 315 DFERIWIHGEKGFLTME-TRLKLGIPIYKKVLDELNEIARRVGVKGLEV 362
+ + G+K M+ + G+ + L L E+A ++ L++
Sbjct: 396 VGKPPMVPGDKELQHMKMVDEQGGLTMSSCTLSVLEELATEFDIEPLKM 444
>sp|P44995|YIAK_HAEIN HYPOTHETICAL PROTEIN HI1031 >gi|1074616|pir||C64165 hypothetical
protein HI1031 - Haemophilus influenzae (strain Rd KW20)
>gi|1574063|gb|AAC22691.1| (U32783) conserved
hypothetical protein [Haemophilus influenzae Rd]
Length = 332
Score = 140 bits (349), Expect = 3e-32
Identities = 101/344 (29%), Positives = 169/344 (48%), Gaps = 16/344 (4%)
Query: 12 VRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGG 71
+R+ DEL + RVL EE A+ A G SHG+ R R++ + G
Sbjct: 1 MRVSYDELKNEFKRVLLDRQLTEELAEECATAFTDTTQAGAYSHGINRFPRFIQQLEQGD 60
Query: 72 I--NLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYG 129
I N P K++ G S D + G + K M AIE A + G+G++A+RN+NH+
Sbjct: 61 IVPNAIPT-KVLSLG-SIEQWDAHQAIGNLTAKKMMDRAIELASQHGVGVIALRNANHWM 118
Query: 130 IAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSV 189
G Y AAE+G IGI TN+ ++ P G E +GTNP+ +A PT ++DM+ S+
Sbjct: 119 RGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLIIAVPTT-PITMVDMSCSM 177
Query: 190 VPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLS 249
G LEV+R G + + +G +TR V LLP+G + KG GLS
Sbjct: 178 YSYGMLEVHRLAGRQTFVDAGFDDEGNLTRDPSIVEKNRRLLPMGFW-------KGSGLS 230
Query: 250 LMVDILSGILSGGTWSKHVKNTNEKNSNVC--HFFMAINIEHFTPLEEFKGRMSEMINEI 307
+++D+++ +LS G V T +KN C F+AI ++ + +++ +++ +
Sbjct: 231 IVLDMIATLLSNG--ESTVAVTEDKNDEYCVSQVFIAIEVDRLIDGKSKDEKLNRIMDYV 288
Query: 308 KNSRKHPDFERIWIHGEKGFLTMETRLKLGIPIYKKVLDELNEI 351
K + + + + + G + + GIP+ ++V +L +
Sbjct: 289 KTAERSDPTQAVRLPGHEFTTILSDNQTNGIPVDERVWAKLKTL 332
>pir||S47796 hypothetical protein o332 - Escherichia coli
>gi|466713|gb|AAB18552.1| (U00039) No definition line
found [Escherichia coli]
Length = 332
Score = 137 bits (341), Expect = 2e-31
Identities = 89/329 (27%), Positives = 157/329 (47%), Gaps = 8/329 (2%)
Query: 12 VRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGG 71
+++ ++L + RVL G E A A+ G+ SHGV R R++ + +G
Sbjct: 1 MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSHGVNRFPRFIQQLENGD 60
Query: 72 INLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIA 131
I + K I + D G + K M AIE A GIG+VA+RN+NH+
Sbjct: 61 IIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRG 120
Query: 132 GYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVP 191
G Y AAE+G IGI TNS ++ P G E +GTNP+ +A P+ ++DM+ S+
Sbjct: 121 GXYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPST-PITMVDMSMSMFS 179
Query: 192 IGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLM 251
G LEV R G ++P + +G +T+ + +LP+G + KG G+S++
Sbjct: 180 YGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYW-------KGSGMSIV 232
Query: 252 VDILSGILSGGTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSR 311
+D+++ +LS G V N + F+AI ++ ++ +++ + ++
Sbjct: 233 LDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRDAKLQRIMDYVTSAE 292
Query: 312 KHPDFERIWIHGEKGFLTMETRLKLGIPI 340
+ + + I + G + + + GI +
Sbjct: 293 RADENQAIRLPGHEFTTLLAENRRNGITV 321
>sp|P37672|YIAK_ECOLI HYPOTHETICAL 36.6 KDA PROTEIN IN AVTA-SELB INTERGENIC REGION (O332)
>gi|7449809|pir||A65157 hypothetical protein b3575 -
Escherichia coli (strain K-12)
>gi|1790000|gb|AAC76599.1| (AE000435) putative
dehydrogenase [Escherichia coli K12]
Length = 332
Score = 136 bits (340), Expect = 3e-31
Identities = 89/329 (27%), Positives = 157/329 (47%), Gaps = 8/329 (2%)
Query: 12 VRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGG 71
+++ ++L + RVL G E A A+ G+ SHGV R R++ + +G
Sbjct: 1 MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSHGVNRFPRFIQQLENGD 60
Query: 72 INLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIA 131
I + K I + D G + K M AIE A GIG+VA+RN+NH+
Sbjct: 61 IIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRG 120
Query: 132 GYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAPTKGKPFLLDMATSVVP 191
G Y AAE+G IGI TNS ++ P G E +GTNP+ +A P+ ++DM+ S+
Sbjct: 121 GSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPST-PITMVDMSMSMFS 179
Query: 192 IGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLM 251
G LEV R G ++P + +G +T+ + +LP+G + KG G+S++
Sbjct: 180 YGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYW-------KGSGMSIV 232
Query: 252 VDILSGILSGGTWSKHVKNTNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSR 311
+D+++ +LS G V N + F+AI ++ ++ +++ + ++
Sbjct: 233 LDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRDAKLQRIMDYVTSAE 292
Query: 312 KHPDFERIWIHGEKGFLTMETRLKLGIPI 340
+ + + I + G + + + GI +
Sbjct: 293 RADENQAIRLPGHEFTTLLAENRRNGITV 321
>pir||B83488 probable L-malate dehydrogenase PA1252 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9947184|gb|AAG04641.1|AE004555_1 (AE004555) probable
L-malate dehydrogenase [Pseudomonas aeruginosa]
Length = 334
Score = 133 bits (332), Expect = 3e-30
Identities = 80/251 (31%), Positives = 120/251 (46%), Gaps = 9/251 (3%)
Query: 12 VRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLKRYVDGILSGG 71
+R+ DE+ VR+L + E + VAD L+ + SHG+ RL + + +G
Sbjct: 2 IRMTLDEVRELAVRILRRHAFSEAHVQAVADTLVAGERDECASHGIWRLLGCIATLKAGK 61
Query: 72 INLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVAVRNSNHYGIA 131
++ ++ P +D GF Q + +EKAR GI +AV H+
Sbjct: 62 VSADAEPELHDIAPGLLRVDAHGGFSQCAFRLGLPHLLEKARSQGIAAMAVNRCVHFSAL 121
Query: 132 GYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAP-TKGKPFLLDMATSVV 190
E G++ ++ T S VAP GG + I GTNPIA P G PF+ D ATS V
Sbjct: 122 WVEVEALTEAGLVALATTPSHAWVAPAGGRKPIFGTNPIAFGWPRPDGPPFVFDFATSAV 181
Query: 191 PIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSL 250
G+++++ R G+ IP GW ++ +GE T G L GGHKG L+
Sbjct: 182 ARGEIQLHERAGKPIPLGWGVDEQGEPTTDASAALRGAML--------TFGGHKGSALAA 233
Query: 251 MVDILSGILSG 261
MV++L+G L G
Sbjct: 234 MVELLAGPLIG 244
>pir||T20825 hypothetical protein F13D12.1 - Caenorhabditis elegans
Length = 355
Score = 88.5 bits (216), Expect = 1e-16
Identities = 55/176 (31%), Positives = 94/176 (53%), Gaps = 16/176 (9%)
Query: 195 LEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGELLGGHKGYGLSLMVDI 254
+E+ RK +P W + G+ T +V GG LLPLGG E+ GG+KGYGLS M++I
Sbjct: 22 VELAARKENPVPLSWGVGEGGKETTDPTKVLYGGGLLPLGGV-EVSGGYKGYGLSSMIEI 80
Query: 255 LSGILSGGTWSKHVKN--TNEKNSNVCHFFMAINIEHFTPLEEFKGRMSEMINEIKNSRK 312
GIL+G W HV+ + + +++ F+AI+ E F P F R+ + + ++
Sbjct: 81 FCGILAGAHWGPHVRKWMSTKSEADLGQCFVAIDPEAFAP--GFADRLQDFMQTMRALPT 138
Query: 313 HPDFERIWIHGEKGFLTMETRLKL------GIPIYKKVLDELNEIARRVGVKGLEV 362
++ + G+ ME R + GIP +K + +N++A ++GVK +++
Sbjct: 139 SSPSFKVEVAGD-----MERRHEALVEQLGGIPYHKNQITFVNDLAAKLGVKTVDL 189
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.141 0.408
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 141535649
Number of Sequences: 2977
Number of extensions: 6471448
Number of successful extensions: 15109
Number of sequences better than 1.0e-10: 23
Number of HSP's better than 0.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 15029
Number of HSP's gapped (non-prelim): 23
length of query: 362
length of database: 189,106,746
effective HSP length: 55
effective length of query: 307
effective length of database: 156,189,961
effective search space: 47950318027
effective search space used: 47950318027
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)