BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1792 (mae) DE:malate oxidoreductase (mae)
(422 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G75131 malate oxidoreductase (mae) PAB1792 - Pyrococcus aby... 839 0.0
pir||H71072 probable malate dehydrogenase (oxaloacetate-decarbox... 700 0.0
gi|11499316 malate oxidoreductase (mae) [Archaeoglobus fulgidus]... 452 e-126
dbj|BAB06887.1| (AP001517) malic enzyme (malate dehydrogenase) [... 403 e-111
sp|P16468|MAOX_BACST NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|659... 394 e-108
pir||G72731 probable malate oxidoreductase APE0392 - Aeropyrum p... 390 e-107
pir||C70001 malate dehydrogenase homolog ytsJ - Bacillus subtili... 383 e-105
gb|AAK42976.1| NAD-dependent malic enzyme (malate oxidoreductase... 381 e-105
emb|CAC11598.1| (AL445064) malate oxidoreductase (malic enzyme) ... 377 e-103
gb|AAB07709.1| (U35659) malic enzyme [Streptococcus bovis] 372 e-102
dbj|BAB04118.1| (AP001508) malate oxidoreductase (NAD) (malic en... 369 e-101
emb|CAB72197.1| (AL138851) putative malate oxidoreductase [Strep... 369 e-101
pir||S74511 malic enzyme - Synechocystis sp. (strain PCC 6803) >... 362 5e-99
pir||C72364 malate oxidoreductase - Thermotoga maritima (strain ... 360 2e-98
emb|CAC05958.1| (AL391763) putative malic enzyme [Streptomyces c... 355 5e-97
gb|AAF43042.1|AF234166_1 (AF234166) malic enzyme [Entamoeba hist... 349 3e-95
pir||H81336 malate dehydrogenase (oxaloacetate-decarboxylating) ... 341 8e-93
sp|P76558|MAO2_ECOLI NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|7... 339 4e-92
dbj|BAA16338.1| (D90875) PUTATIVE MALATE OXIDOREDUCTASE (NAD) (E... 339 4e-92
pir||H82737 malate oxidoreductase XF0977 [imported] - Xylella fa... 334 1e-90
pir||E83014 malic enzyme PA5046 [imported] - Pseudomonas aerugin... 333 3e-90
sp|P43837|MAO2_HAEIN NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|1... 328 7e-89
gb|AAF37577.1|AF234535_1 (AF234535) malic enzyme [Corynebacteriu... 326 2e-88
pir||D82047 probable malate oxidoreductase VC2681 [imported] - V... 326 4e-88
gb|AAG19885.1| (AE005072) malate dehydrogenase; Mdh [Halobacteri... 326 4e-88
pir||T00127 hypothetical protein 5 - Leptospira interrogans >gi|... 324 8e-88
pir||C81173 malate oxidoreductase (NAD) NMB0671 [imported] - Nei... 323 2e-87
pir||A81933 probable malate dehydrogenase (oxaloacetate-decarbox... 323 2e-87
sp|O30808|MAO2_RHIME NADP-DEPENDENT MALIC ENZYME (NADP-ME) 319 5e-86
gb|AAB82460.1| (AF017444) NADP-dependent malic enzyme; TME [Sino... 317 1e-85
sp|O30807|MAO1_RHIME NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|258... 315 7e-85
sp|Q9ZDF6|MAO2_RICPR PROBABLE NADP-DEPENDENT MALIC ENZYME (NADP-... 313 2e-84
pir||T46727 probable malic enzyme [imported] - Weissella parames... 294 2e-78
emb|CAA71638.1| (Y10621) malic enzyme [Leuconostoc mesenteroides] 293 3e-78
sp|P54572|MAO1_BACSU PROBABLE MALATE OXIDOREDUCTASE [NAD] (MALIC... 285 6e-76
dbj|BAA16337.1| (D90874) PUTATIVE MALATE OXIDOREDUCTASE (NAD) (E... 145 7e-34
pir||T02763 probable malate dehydrogenase (oxaloacetate-decarbox... 118 9e-26
pir||D83211 probable malic enzyme PA3471 [imported] - Pseudomona... 116 5e-25
emb|CAA56354.1| (X80051) NADP dependent malic enzyme [Phaseolus ... 115 8e-25
pir||S48198 malic enzyme, NADP-dependent - kidney bean >gi|74276... 115 8e-25
sp|P12628|MAOX_PHAVU NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|1... 114 2e-24
sp|P16243|MAOC_MAIZE NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PR... 113 4e-24
sp|P36444|MAOC_FLAPR NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PR... 113 5e-24
prf||1701292A NADP dependent malic enzyme [Flaveria trinervia] 112 7e-24
pir||T48526 NADP dependent malic enzyme-like protein - Arabidops... 111 1e-23
pir||C75581 malate oxidoreductase - Deinococcus radiodurans (str... 111 2e-23
pir||JC5967 malate dehydrogenase (oxaloacetate-decarboxylating) ... 110 3e-23
pir||H75256 malate oxidoreductase - Deinococcus radiodurans (str... 110 3e-23
sp|P22178|MAOC_FLATR NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PR... 110 3e-23
gb|AAF73006.1|AF262997_1 (AF262997) NADP-dependent malic protein... 110 3e-23
pir||T06402 malate dehydrogenase (oxaloacetate-decarboxylating) ... 109 4e-23
gb|AAF09064.1|AC011717_31 (AC011717) putative malate oxidoreduct... 109 4e-23
emb|CAC18164.1| (AL451013) probable malate dehydrogenase (oxaloa... 109 4e-23
pir||A82232 malate oxidoreductase VC1188 [imported] - Vibrio cho... 109 8e-23
pir||T06401 malate dehydrogenase (oxaloacetate-decarboxylating) ... 107 3e-22
gb|AAD40139.1|AF149413_20 (AF149413) similar to malate dehydroge... 107 3e-22
gi|4505143 malic enzyme 1, NADP(+)-dependent, cytosolic; Malic e... 106 4e-22
gb|AAC50613.1| (U43944) cytosolic NADP(+)-dependent malic enzyme... 106 4e-22
gb|AAB08874.1| (U67426) malate dehydrogenase [Vitis vinifera] 106 4e-22
sp|P37223|MAOX_MESCR NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|1... 106 5e-22
gb|AAC62126.1| (AC005169) malate oxidoreductase (malic enzyme) [... 106 5e-22
gb|AAD10504.1| (U39958) NADP-malic enzyme [Zea mays] 106 5e-22
sp|P43279|MAOC_ORYSA NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PR... 105 1e-21
dbj|BAA74735.1| (AB016804) NADP-malic enzyme [Aloe arborescens] 104 1e-21
sp|P37222|MAOC_LYCES NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST (N... 104 2e-21
emb|CAB64263.1| (AJ251546) malate dehydrogenase (NADP-dependent ... 104 2e-21
gb|AAF56674.1| (AE003759) Mdh gene product [Drosophila melanogas... 104 2e-21
dbj|BAB03427.1| (AP002816) ESTs AU091509(C10754),D24805(R2584),C... 104 2e-21
sp|P13697|MAOX_RAT NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|659... 104 2e-21
gi|7106353 malic enzyme 1, soluble [Rattus norvegicus] >gi|20529... 104 2e-21
sp|P28227|MAOX_ANAPL NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|1... 103 3e-21
pir||JC4160 malate dehydrogenase (oxaloacetate-decarboxylating) ... 103 3e-21
gi|7019429 NADP-dependent malic enzyme [Homo sapiens] >gi|133538... 103 3e-21
sp|P34105|MAOX_POPTR NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|2... 102 6e-21
sp|P27443|MAOM_ASCSU NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL P... 102 8e-21
pir||S29742 malate dehydrogenase (oxaloacetate-decarboxylating) ... 102 8e-21
sp|P40927|MAOX_COLLI NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|6... 102 8e-21
prf||1803524A malic enzyme [Populus trichocarpa x Populus deltoi... 102 1e-20
gi|6678912 malic enzyme, supernatant [Mus musculus] >gi|126738|s... 101 2e-20
sp|P26616|MAO1_ECOLI NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|743... 101 2e-20
emb|CAA50716.1| (X71897) malolactic enzyme [Lactococcus lactis] 100 3e-20
pir||S43057 malolactic enzyme - Lactococcus lactis >gi|2130997|e... 100 3e-20
emb|CAB66003.1| (AJ132257) NADP-dependent malate dehydrogenase (... 100 3e-20
sp|P37225|MAON_SOLTU NAD-DEPENDENT MALIC ENZYME 59 KDA ISOFORM, ... 100 4e-20
sp|Q48662|MLES_LACLA MALOLACTIC ENZYME >gi|541361|pir||S38728 ma... 100 4e-20
sp|P45868|MAO2_BACSU PROBABLE NAD-DEPENDENT MALIC ENZYME (NAD-ME... 100 4e-20
pir||S18826 malate dehydrogenase (oxaloacetate-decarboxylating) ... 100 5e-20
sp|Q29558|MAOX_PIG NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|106... 99 7e-20
sp|P51615|MAOX_VITVI NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|5... 98 1e-19
gb|AAC13636.2| (AF058919) F6N23.16 gene product [Arabidopsis tha... 98 2e-19
pir||T01221 malate dehydrogenase (decarboxylating) (EC 1.1.1.39)... 98 2e-19
gb|AAG13628.1|AC078840_19 (AC078840) putative mitochondrial NAD+... 97 3e-19
sp|P71880|MAOX_MYCTU PUTATIVE MALATE OXIDOREDUCTASE [NAD] (MALIC... 95 2e-18
sp|Q48796|MLES_OENOE MALOLACTIC ENZYME >gi|7431227|pir||T13496 m... 94 3e-18
sp|P40375|MAOX_SCHPO NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|629... 92 8e-18
pir||T07135 malate dehydrogenase (oxaloacetate-decarboxylating) ... 90 3e-17
gb|AAF43601.1|AF187998_1 (AF187998) malic enzyme [Drosophila mel... 90 4e-17
gb|AAF43602.1|AF187999_1 (AF187999) malic enzyme [Drosophila mel... 90 4e-17
gb|AAF43603.1|AF188000_1 (AF188000) malic enzyme [Drosophila mel... 90 4e-17
gb|AAF54859.1| (AE003697) Men gene product [alt 1] [Drosophila m... 90 4e-17
gb|AAF54860.1| (AE003697) Men gene product [alt 2] [Drosophila m... 90 4e-17
gi|5729920 malic enzyme 3, NADP(+)-dependent, mitochondrial; mal... 88 2e-16
pir||S69778 adhesin AP65-1 precursor - Trichomonas vaginalis >gi... 88 2e-16
gb|AAA92715.1| (U16837) malic enzyme subunit B proprotein [Trich... 88 2e-16
pir||D69655 malate dehydrogenase malS - Bacillus subtilis >gi|22... 88 2e-16
sp|P37224|MAOM_AMAHP NAD-DEPENDENT MALIC ENZYME 65 KDA ISOFORM, ... 88 2e-16
pir||S17455 malate dehydrogenase (oxaloacetate-decarboxylating) ... 87 3e-16
gi|11438058 malic enzyme 3, NADP(+)-dependent, mitochondrial [Ho... 87 3e-16
emb|CAB52344.1| (AL049699) dJ747H23.1 (malic enzyme 1, soluble (... 87 4e-16
gb|AAF68116.1|AC010793_11 (AC010793) F20B17.18 [Arabidopsis thal... 83 7e-15
gb|AAA91133.1| (U35243) AP65-3 adhesin [Trichomonas vaginalis] 82 9e-15
gb|AAB39711.1| (U73780) malic enzyme [Pseudomonas aeruginosa] 82 2e-14
pir||S69779 adhesin AP65-2 precursor - Trichomonas vaginalis >gi... 79 1e-13
emb|CAA39419.1| (X55956) alternative [Escherichia coli] 78 2e-13
pir||S38951 sfcA protein - Escherichia coli 78 2e-13
emb|CAA39420.1| (X55956) alternative [Escherichia coli] 78 2e-13
emb|CAA39421.1| (X55956) alternative [Escherichia coli] 78 2e-13
emb|CAA39422.1| (X55956) alternative [Escherichia coli] 78 2e-13
emb|CAA39423.1| (X55956) alternative [Escherichia coli] 78 2e-13
gb|AAC49572.1| (U62041) malic enzyme precursor [Neocallimastix f... 76 5e-13
pir||T45208 probable malate dehydrogenase (EC 1.1.1.37) mox [imp... 76 7e-13
>pir||G75131 malate oxidoreductase (mae) PAB1792 - Pyrococcus abyssi (strain
Orsay) >gi|5458279|emb|CAB49768.1| (AJ248285) malate
oxidoreductase (mae) [Pyrococcus abyssi]
Length = 422
Score = 839 bits (2143), Expect = 0.0
Identities = 422/422 (100%), Positives = 422/422 (100%)
Query: 1 MDPLKFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
MDPLKFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV
Sbjct: 1 MDPLKFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT
Sbjct: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS
Sbjct: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT
Sbjct: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
Query: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK
Sbjct: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
Query: 301 KAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE 360
KAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE
Sbjct: 301 KAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE 360
Query: 361 ENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKLISLWDDILGEVADK 420
ENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKLISLWDDILGEVADK
Sbjct: 361 ENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKLISLWDDILGEVADK 420
Query: 421 LS 422
LS
Sbjct: 421 LS 422
>pir||H71072 probable malate dehydrogenase (oxaloacetate-decarboxylating) (EC
1.1.1.38), NAD-linked - Pyrococcus horikoshii
>gi|3257695|dbj|BAA30378.1| (AP000005) 439aa long
hypothetical malate oxidoreductase (NAD) [malic enzyme]
[Pyrococcus horikoshii]
Length = 439
Score = 700 bits (1786), Expect = 0.0
Identities = 346/422 (81%), Positives = 386/422 (90%), Gaps = 5/422 (1%)
Query: 4 LKFHKG-----GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSV 58
L+FHK GKI VI KV L++RE L+LAYTPGVAEPCK IA+DP KVYEYTSKGN V
Sbjct: 7 LEFHKNNFPGNGKIEVIPKVSLESREELTLAYTPGVAEPCKEIARDPGKVYEYTSKGNLV 66
Query: 59 AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAIS 118
AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGG+DAFPIMI EQ+P+KFI++VKAI+
Sbjct: 67 AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGVDAFPIMIKEQEPNKFIDIVKAIA 126
Query: 119 PTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDE 178
PTFGGINLEDIASPKCFYIL++LREELDIPVFHDDQQGTAAVVLAGL+NAL+VVGK++ E
Sbjct: 127 PTFGGINLEDIASPKCFYILERLREELDIPVFHDDQQGTAAVVLAGLLNALKVVGKKISE 186
Query: 179 ISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLE 238
I++ALFGAGAAGFATLRIL +AGVKP NVRVVELVNGKP +LT DLDLEKLFPYRGWLL+
Sbjct: 187 ITLALFGAGAAGFATLRILTEAGVKPENVRVVELVNGKPRILTSDLDLEKLFPYRGWLLK 246
Query: 239 KTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDE 298
KTN E IEGGP+EALK ADVLISFTRPGPGVIKP+WI +M++D IVFPLANPVPEILP+E
Sbjct: 247 KTNGENIEGGPQEALKDADVLISFTRPGPGVIKPQWIEKMNEDAIVFPLANPVPEILPEE 306
Query: 299 AKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
AKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRAR+ITD MII AAKAIAS+VEEP
Sbjct: 307 AKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARTITDSMIIAAAKAIASIVEEP 366
Query: 359 SEENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKLISLWDDILGEVA 418
SEENIIPSP NPIVYA+EARAVAEEAMKEGVAR KVKGE +EE T++LI +++++ +
Sbjct: 367 SEENIIPSPLNPIVYAREARAVAEEAMKEGVARTKVKGEWVEEHTIRLIEFYENVIAPIN 426
Query: 419 DK 420
K
Sbjct: 427 KK 428
>gi|11499316 malate oxidoreductase (mae) [Archaeoglobus fulgidus]
>gi|7431218|pir||F69465 malate oxidoreductase (mae)
homolog - Archaeoglobus fulgidus
>gi|2648827|gb|AAB89524.1| (AE000984) malate
oxidoreductase (mae) [Archaeoglobus fulgidus]
Length = 456
Score = 452 bits (1150), Expect = e-126
Identities = 230/402 (57%), Positives = 297/402 (73%), Gaps = 5/402 (1%)
Query: 9 GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRIL 68
GGKI ++ KVP++T + S+ YTPGVAE C IA+D EK Y+YT+K N +A+++DGSR L
Sbjct: 38 GGKIEILPKVPIRTLDDFSIWYTPGVAEVCNEIAEDEEKAYDYTNKWNLIAIITDGSRTL 97
Query: 69 GLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLED 128
GLGNIGP A +PVMEGKALLFK G +DAFPI + EQD D+FIE+VKAISPTFGG+NLED
Sbjct: 98 GLGNIGPKAAMPVMEGKALLFKFLGAVDAFPIALAEQDADRFIEIVKAISPTFGGVNLED 157
Query: 129 IASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGA 188
I+SPKCFYIL++L++ELDIPV+HDDQQGTA LA LINAL++VGK + E+ +++ G GA
Sbjct: 158 ISSPKCFYILERLQKELDIPVWHDDQQGTATATLAALINALKIVGKDISEVKISIIGVGA 217
Query: 189 AGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGG 248
A FAT+R+L AG P + VV+ K ++ DL++ Y+ + +TN E GG
Sbjct: 218 ANFATIRLLKAAGADPKKMFVVD---SKGILHPDRADLKEKKGYKWQVAIETNGERRTGG 274
Query: 249 PEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARIVA 308
EEA++G DVLI+ ++PGP VIK EWI EM+DD IVFPLANP PEI P EAK AGARIV
Sbjct: 275 MEEAIRGTDVLIAASKPGPDVIKKEWIAEMNDDAIVFPLANPTPEIWPWEAKDAGARIVG 334
Query: 309 TGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP--SEENIIPS 366
TGRSD+PNQ+NN L FPG+FRGAL VRAR ITD+M + AA A+A E SE+ IIP
Sbjct: 335 TGRSDFPNQVNNSLVFPGVFRGALTVRARKITDEMCLAAANALAEFAERNGLSEDYIIPR 394
Query: 367 PFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKLIS 408
V+ + A AV +A++ G+AR++++ EEI E +IS
Sbjct: 395 MTEKEVFPEVATAVGVKAVELGLARLELREEEIYEIAELMIS 436
>dbj|BAB06887.1| (AP001517) malic enzyme (malate dehydrogenase) [Bacillus
halodurans]
Length = 410
Score = 403 bits (1025), Expect = e-111
Identities = 213/403 (52%), Positives = 283/403 (69%), Gaps = 19/403 (4%)
Query: 10 GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILG 69
GK+ SKVP++ LSLAY+PGVAEPCK I +D E VYEYT KGN VAVVS+G+ +LG
Sbjct: 17 GKLESKSKVPVRNARDLSLAYSPGVAEPCKEIFEDVETVYEYTMKGNMVAVVSNGTAVLG 76
Query: 70 LGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLEDI 129
LGNIGP A LPVMEGKA+LFK F G+DAFPI + D D+ +E VK + PTFGG+NLEDI
Sbjct: 77 LGNIGPEASLPVMEGKAVLFKSFAGVDAFPICLRTTDVDQIVETVKLLEPTFGGVNLEDI 136
Query: 130 ASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAA 189
A+P CF I ++L++E +IPVFHDDQ GTA V LAGLINAL++ GKR+ EI V GAGAA
Sbjct: 137 AAPSCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLINALKLTGKRMSEIKVVANGAGAA 196
Query: 190 GFATLRILVKAGVKPGNVRVVE----LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGI 245
G A ++++ + GV+ ++ + + + +G+P + D+ E + + TN + I
Sbjct: 197 GIAIIKLMQRMGVR--DIIMCDSKGAIYDGRPYGM-NDMKAE--------VAKWTNRDKI 245
Query: 246 EGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGAR 305
+GG + LKGADV I + G + E I M+DDPI+F +ANPVPEI+P+EAK AGA+
Sbjct: 246 DGGLGDVLKGADVFIGVS--VAGALTKEMIENMNDDPIIFAMANPVPEIMPEEAKAAGAK 303
Query: 306 IVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENI 363
++ TGRSD+PNQ+NN+L FPGIFRGALD RA I ++M + A AIA +V E+ + +
Sbjct: 304 VIGTGRSDFPNQVNNVLAFPGIFRGALDARATHINEEMKVAAVHAIADLVAEEDLDPDYV 363
Query: 364 IPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKL 406
IP+PF+P V A AVA+ AM+ GVAR KV E+E +T KL
Sbjct: 364 IPAPFDPRVAPAVAAAVAKTAMETGVARRKVDPAEVEAKTKKL 406
>sp|P16468|MAOX_BACST NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|65941|pir||DEBSXS malate
dehydrogenase (oxaloacetate-decarboxylating) (EC
1.1.1.38) - Bacillus stearothermophilus
>gi|143165|gb|AAA22585.1| (M19485) malic enzyme (EC
1.1.1.38) [Bacillus stearothermophilus]
Length = 478
Score = 394 bits (1001), Expect = e-108
Identities = 211/387 (54%), Positives = 277/387 (71%), Gaps = 13/387 (3%)
Query: 7 HKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSR 66
H GGKI SK+P+KTR+ LS YTPGVA C AIA+DP K Y T K N+VAVVSDG+
Sbjct: 91 HIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDPRKAYSLTIKRNTVAVVSDGTA 150
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINL 126
+LGLG+IGP A +PVMEGKA+LFK F G+DAFPI + +D ++ I++VKAI+P FGGINL
Sbjct: 151 VLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLDTKDTEEIIQIVKAIAPAFGGINL 210
Query: 127 EDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGA 186
EDI++P+CF I +L+EELDIPVFHDDQ GTA V+LAGL+NAL++V K+L++I V L G
Sbjct: 211 EDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVDKKLEDIKVVLTGI 270
Query: 187 GAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIE 246
GAAG A +IL+ AGV+ N+ V+ + + +D E PY + TN + ++
Sbjct: 271 GAAGIACTKILLAAGVR--NIIGVD----RHGAIHRDETYEN--PYWQEYAQLTNPDNLK 322
Query: 247 GGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARI 306
G + + GADV I + PG++K E +++M+ DPIVF +ANP+PEI P+ A+ R+
Sbjct: 323 GSLSDVIAGADVFIGVS--APGILKVEDVKKMARDPIVFAMANPIPEIDPELAEPY-VRV 379
Query: 307 VATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENII 364
+ATGRSDYPNQINN+L FPGIFRGALD RAR I ++M + AAKAIASVV +E +E II
Sbjct: 380 MATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVTEDELNETYII 439
Query: 365 PSPFNPIVYAKEARAVAEEAMKEGVAR 391
PS FN V + +AV E A + GVAR
Sbjct: 440 PSVFNSKVVERVRQAVVEAAYRTGVAR 466
>pir||G72731 probable malate oxidoreductase APE0392 - Aeropyrum pernix (strain
K1) >gi|5104031|dbj|BAA79347.1| (AP000059) 446aa long
hypothetical malate oxidoreductase [Aeropyrum pernix]
Length = 446
Score = 390 bits (991), Expect = e-107
Identities = 215/401 (53%), Positives = 275/401 (67%), Gaps = 12/401 (2%)
Query: 9 GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRIL 68
GGKI V+ KVP+ E ++ YTPGVAE I KDP++ +E TS+ N VA+V+D +R+L
Sbjct: 23 GGKIEVMPKVPVSGVEDFAIWYTPGVAEASIRINKDPDESFELTSRWNMVAIVTDATRVL 82
Query: 69 GLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLED 128
GLG + P A PVMEGKAL+FK FGG+DA PI+ ++P F +VK + P+FGG+NLED
Sbjct: 83 GLGKLLPEAAYPVMEGKALIFKLFGGVDAVPIVHRRREPGAFASLVKDLEPSFGGVNLED 142
Query: 129 IASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGA 188
I SPKCFY+LD LR EL+IPV+HDDQQGTAA LAGLINA ++VGK L E + L GAGA
Sbjct: 143 IESPKCFYLLDFLRMELEIPVWHDDQQGTAAASLAGLINAFKIVGKNLRESRIVLLGAGA 202
Query: 189 AGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLF---PYRGWLLEKTNDEGI 245
A A R L GV P N+ VV+ K ++ + D+++L Y+ + +T G+
Sbjct: 203 ANIALYRFLKAYGVNPRNIVVVD---SKGVLHPEREDMDRLLFSNRYKYEIALETEGGGL 259
Query: 246 EGGP--EEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAG 303
G EEAL+GAD L++ +RPGPGV+K EWIR MS DPIVF LANP PEI P EA+ AG
Sbjct: 260 APGSSLEEALRGADALVAASRPGPGVVKKEWIRGMSRDPIVFALANPTPEIWPWEARDAG 319
Query: 304 ARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP---SE 360
AR+VATGRSD PNQ+NN L FPG+FRGALDVR+R+ITD M I AA+ IA EE E
Sbjct: 320 ARVVATGRSDLPNQVNNALVFPGVFRGALDVRSRAITDGMAIAAAEEIARYTEEEIGIEE 379
Query: 361 ENIIPSPFNPIVYAKEARAVAEEAMKEGVAR-VKVKGEEIE 400
+ I+P + V+ + A AVA A +E VAR + EE+E
Sbjct: 380 DRILPPITDWQVHYRVAAAVAWAASREKVARKPRTYAEELE 420
>pir||C70001 malate dehydrogenase homolog ytsJ - Bacillus subtilis
>gi|2293261|gb|AAC00339.1| (AF008220) YtsJ [Bacillus
subtilis] >gi|2635387|emb|CAB14882.1| (Z99118) similar
to malate dehydrogenase [Bacillus subtilis]
Length = 410
Score = 383 bits (973), Expect = e-105
Identities = 204/409 (49%), Positives = 280/409 (67%), Gaps = 13/409 (3%)
Query: 2 DPLKFHK--GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVA 59
+ L HK GK+ SKV ++ + LSLAY+PGVAEPCK I +D KVY+YT KGN VA
Sbjct: 6 EALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMKGNMVA 65
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISP 119
VV+DG+ +LGLGNIGP A LPVMEGKA+LFK F G+DAFPI + D DK +E VK + P
Sbjct: 66 VVTDGTAVLGLGNIGPEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVKLLEP 125
Query: 120 TFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEI 179
TFGG+NLEDIA+P CF I ++L++E +IPVFHDDQ GTA V +AGL+NAL++ GK + I
Sbjct: 126 TFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGKSMSSI 185
Query: 180 SVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEK 239
V GAGAAG A +++L G VR + + + K + + + + + +
Sbjct: 186 KVVANGAGAAGIAIIKLLHHYG-----VRDIVMCDSKGAIY--EGRPNGMNDVKNEVAKF 238
Query: 240 TNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEA 299
TN + +G ++ + ADV I + G + E ++ M+ DPI+F +ANP PEI+P++A
Sbjct: 239 TNQDRKDGSLKDVIVDADVFIGVS--VAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDA 296
Query: 300 KKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EE 357
++AGA +V TGRSD+PNQ+NN+L FPGIFRGALDVRA I ++M I A +AIAS+V +E
Sbjct: 297 REAGASVVGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDE 356
Query: 358 PSEENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKL 406
S + +IP+PF+ V A+AVA+ AM+ GVAR+ V EE+ E+T KL
Sbjct: 357 LSADYVIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEKTRKL 405
>gb|AAK42976.1| NAD-dependent malic enzyme (malate oxidoreductase) [Sulfolobus
solfataricus]
Length = 432
Score = 381 bits (969), Expect = e-105
Identities = 207/398 (52%), Positives = 271/398 (68%), Gaps = 11/398 (2%)
Query: 10 GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILG 69
GKI + KVP+ + +L YTPGVAE KAI KDP+K +E TS+ N++AVV+DG+R+LG
Sbjct: 18 GKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELTSRWNTIAVVTDGTRVLG 77
Query: 70 LGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLEDI 129
LGNIGP A +PVMEGK+LLFK GG+DA P+ I + D+F VVKA+ +FGGINLEDI
Sbjct: 78 LGNIGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTNVVKALETSFGGINLEDI 137
Query: 130 ASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAA 189
SPKCFYIL+KL+ L+IPV+HDDQQGTAA +LAGLINA+ + K E V +GAGA+
Sbjct: 138 ESPKCFYILEKLQSMLNIPVWHDDQQGTAAAILAGLINAMILTNKDPKESKVVFYGAGAS 197
Query: 190 GFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLE---KTND-EGI 245
A RIL K G K N + +++ K + D++ L + W E KTN E
Sbjct: 198 NIAAARILAKYGFKYEN---IVMIDSKGPLYADREDVDHLMLHHKWKYELAIKTNKWEAK 254
Query: 246 EGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGAR 305
E ++A GAD +++ + PGP VIK EWI M+ + IVF LANPVPEI P +AK+AGA+
Sbjct: 255 E--IKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPEIWPQDAKEAGAK 312
Query: 306 IVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP--SEENI 363
IVATGRSD+PNQ+NN L FP IFRG LD RA++ITD+M I A++A+A + +E I
Sbjct: 313 IVATGRSDFPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAKYARDRGINENYI 372
Query: 364 IPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
IP + +EA AVA +A++ G+ARVK EE E
Sbjct: 373 IPRMDEWEAFYEEAAAVASKAVELGLARVKKSREEFRE 410
>emb|CAC11598.1| (AL445064) malate oxidoreductase (malic enzyme) related protein
[Thermoplasma acidophilum]
Length = 467
Score = 377 bits (957), Expect = e-103
Identities = 192/413 (46%), Positives = 282/413 (67%), Gaps = 6/413 (1%)
Query: 10 GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILG 69
GKI ISKVP+++ + S+ YTPGVA + IA DP+ +E T + NS+A+++DG+R+LG
Sbjct: 44 GKIQTISKVPVRSLDDFSIWYTPGVAAVSRKIASDPDLSFELTGRWNSIAILTDGTRVLG 103
Query: 70 LGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLEDI 129
LGNIGP A +PVMEGKAL+F GG++A PI I Q ++F++V KA+ P+FGGINLEDI
Sbjct: 104 LGNIGPEAAMPVMEGKALIFNYLGGVNAIPIPIRVQSKEEFVKVAKALEPSFGGINLEDI 163
Query: 130 ASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAA 189
SPKCF++L+ L+ E++IPV+HDDQ GTA++ LAG+IN+L +VGK++D++ V GAGAA
Sbjct: 164 ESPKCFFLLETLQNEMNIPVWHDDQLGTASITLAGVINSLRIVGKKIDQVKVVFNGAGAA 223
Query: 190 GFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLF---PYRGWLLEKTNDEGIE 246
A + AG K N + LV+ K ++ + D++ L P++ L +TN E ++
Sbjct: 224 NIAAAYLFKAAGFKMKN---MILVDSKGILHPEREDIDALMINNPWKYQLAIETNGERVK 280
Query: 247 GGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARI 306
G A++GAD+LIS + GP I +++M++D IVF LANP+PE+ P EAK+ GARI
Sbjct: 281 GDLSNAIEGADLLISAAKSGPDTIDSRIVKKMNNDAIVFVLANPIPEMWPQEAKENGARI 340
Query: 307 VATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEENIIPS 366
VATGR D+PNQINN L FPG+FRG LD RA+ + D+++ A+ IA+ VE+P+EE I+P+
Sbjct: 341 VATGRGDFPNQINNSLVFPGVFRGVLDARAKGVNFDVMVAASYEIANFVEDPTEEKIVPT 400
Query: 367 PFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTLKLISLWDDILGEVAD 419
+Y + A AVA + ++ G+AR E R ++I +I ++ D
Sbjct: 401 MEEWELYPRLASAVASKTVELGLARKSDSKEGFYRRAREIIESNRNIYSKLMD 453
>gb|AAB07709.1| (U35659) malic enzyme [Streptococcus bovis]
Length = 389
Score = 372 bits (944), Expect = e-102
Identities = 196/381 (51%), Positives = 262/381 (68%), Gaps = 13/381 (3%)
Query: 9 GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRIL 68
GGK+ V KVP++T+ L +AYTPGVA AI + E+ YE T+K N+VAV+SDGS +L
Sbjct: 18 GGKLEVCPKVPIETKADLGIAYTPGVAAVSSAIYEKKERAYELTTKKNTVAVISDGSAVL 77
Query: 69 GLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLED 128
GLGNIGP A +PVMEGKA LFKRF G+D+ P+++ QD ++ I+ VK ++PTFGGINLED
Sbjct: 78 GLGNIGPEAAMPVMEGKAALFKRFAGVDSIPLVLDTQDTEEIIQTVKFLAPTFGGINLED 137
Query: 129 IASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGA 188
I++P+CF I +L +ELDIPVFHDDQ GTA VVLA L N+L+++ K++++I V + G G+
Sbjct: 138 ISAPRCFEIEQRLIDELDIPVFHDDQHGTAIVVLAALYNSLKLINKKIEDIHVVINGGGS 197
Query: 189 AGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGG 248
AG + R + AGVK + +V+ ++ D L P+ + + TN E G
Sbjct: 198 AGLSITRKFLAAGVKH-----IIIVDRTGILSETDTALP---PHHAEIAKLTNREHRTGD 249
Query: 249 PEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARIVA 308
AL+GADV + + PGV+KPEWI++M++ P++F +ANPVPEI PDEA AGA IV
Sbjct: 250 LATALEGADVFVGVS--APGVLKPEWIQQMNEQPVIFAMANPVPEIFPDEALAAGAYIVG 307
Query: 309 TGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPS 366
TGRSD+PNQINN+L FPGIFRGALD RA+ IT +M I AAK IA ++ E + NIIP
Sbjct: 308 TGRSDFPNQINNVLAFPGIFRGALDARAKKITIEMQIAAAKGIAKLIPDNELTPTNIIPD 367
Query: 367 PFNPIVYAKEARAVAEEAMKE 387
PF V AK A+KE
Sbjct: 368 PFQEGV-AKVVAESVRNAVKE 387
>dbj|BAB04118.1| (AP001508) malate oxidoreductase (NAD) (malic enzyme) [Bacillus
halodurans]
Length = 397
Score = 369 bits (938), Expect = e-101
Identities = 197/379 (51%), Positives = 262/379 (68%), Gaps = 12/379 (3%)
Query: 10 GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILG 69
GK+ ++ K+ + + LSL YTPGVA+ CK IA+ P+KV TS+GN VA+V+DG+ +LG
Sbjct: 19 GKLEIVGKMEITDEKDLSLVYTPGVADVCKQIAEQPDKVNSLTSRGNMVAIVTDGTAVLG 78
Query: 70 LGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLEDI 129
LGNIGP A +PVMEGKALLFK+F ID FP+ + QD D+ ++++KA+SPTF GINLEDI
Sbjct: 79 LGNIGPKAAMPVMEGKALLFKKFANIDGFPVCLNTQDTDEIVQIIKALSPTFAGINLEDI 138
Query: 130 ASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAA 189
A+P+CF I ++L+EELDIPVFHDDQ GTA VVLA L NAL+VV K I + + GAGAA
Sbjct: 139 AAPRCFEIEERLKEELDIPVFHDDQHGTAIVVLAALTNALKVVNKLNQPIKMVINGAGAA 198
Query: 190 GFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGP 249
G A +L+ AG K + LV+ + +V + L P + + E TN G++G
Sbjct: 199 GTAIANLLLHAGFKD-----ITLVSLEGVVAKGEAWLN---PMQAKMAEVTNLAGVKGTL 250
Query: 250 EEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARIVAT 309
++A+ ADV I + GPG +K E I+ M+ DPIV LANP+PEI P+EA +AGA +V T
Sbjct: 251 KDAITDADVFIGVS--GPGAMKKEHIQAMAKDPIVLALANPIPEIYPEEAIEAGAAVVGT 308
Query: 310 GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPSP 367
GRSDYPNQ+NNLL FPGIFRGALD A++IT +M I AA AIA V+ +E + IIP
Sbjct: 309 GRSDYPNQVNNLLAFPGIFRGALDAGAKTITMEMKIAAAYAIADVLSDKEVNANMIIPDA 368
Query: 368 FNPIVYAKEARAVAEEAMK 386
+ V + A AVA A++
Sbjct: 369 LDARVAQRVAEAVATTALQ 387
>emb|CAB72197.1| (AL138851) putative malate oxidoreductase [Streptomyces coelicolor
A3(2)]
Length = 471
Score = 369 bits (936), Expect = e-101
Identities = 207/387 (53%), Positives = 263/387 (67%), Gaps = 12/387 (3%)
Query: 7 HKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSR 66
H GGKI + SK P++ R+ LS+ YTPGVA C AIA++PE T K NSVAVV+DGS
Sbjct: 91 HLGGKIEMQSKHPIRNRDDLSMVYTPGVARVCMAIAENPEDARRLTIKRNSVAVVTDGSA 150
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINL 126
+LGLGNIGP A LPVMEGKA LFKRF GIDA+PI + QD D +E+VKAI+P F GINL
Sbjct: 151 VLGLGNIGPKAALPVMEGKAALFKRFAGIDAWPICLDTQDTDAIVEIVKAIAPGFAGINL 210
Query: 127 EDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGA 186
EDI++P+CF I +LRE LDIPVFHDDQ GTA VVLA L NAL VVGK ++ + V + GA
Sbjct: 211 EDISAPRCFEIEARLREALDIPVFHDDQHGTAIVVLAALTNALRVVGKGIENVRVVMSGA 270
Query: 187 GAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRG--WLLEKTNDEG 244
GAAG A L++L+ AGVK VV ++G +V T DL P W+ + TN EG
Sbjct: 271 GAAGTAILKLLLAAGVKNA---VVADIHG--VVHTGRADLVDAAPESALRWIADNTNPEG 325
Query: 245 IEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGA 304
+ G +EA+ GADV I + P V+ E + M+D IVF LANP PE+ P A++ A
Sbjct: 326 LTGTLKEAVHGADVFIGVS--APNVLDGEDVAAMADGAIVFALANPDPEVDPAIARETAA 383
Query: 305 RIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEEN 362
+VATGRSD+PNQINN+L FPG+FRG LD ++R++ +M++ AA A+A+VV +E +
Sbjct: 384 -VVATGRSDFPNQINNVLVFPGVFRGLLDAQSRTVNTEMMLAAAHALANVVTEDEINPNY 442
Query: 363 IIPSPFNPIVYAKEARAVAEEAMKEGV 389
IIPS FN V A AV + A GV
Sbjct: 443 IIPSVFNDKVAGAVAGAVRDAAKAAGV 469
>pir||S74511 malic enzyme - Synechocystis sp. (strain PCC 6803)
>gi|1651735|dbj|BAA16663.1| (D90899) malic enzyme
[Synechocystis sp.]
Length = 463
Score = 362 bits (919), Expect = 5e-99
Identities = 196/386 (50%), Positives = 259/386 (66%), Gaps = 18/386 (4%)
Query: 7 HKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSR 66
H+ GKI V+S++PL ++ L++AYTPGV C+AIA+DPEKVY T K N+VAVV+DGS
Sbjct: 92 HRQGKISVVSRIPLTSQSDLAMAYTPGVGRICRAIAEDPEKVYSLTIKSNTVAVVTDGSA 151
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINL 126
+LGLGN+GP A LPVMEGKA+LFK F +DAFPI + QD ++ I VKAI+P FGG+NL
Sbjct: 152 VLGLGNLGPEAALPVMEGKAMLFKEFAQLDAFPICLDTQDTEEIIRTVKAIAPVFGGVNL 211
Query: 127 EDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGA 186
EDIA+P+CF I +L++EL+IPVFHDDQ GTA V LA L+NAL+ VGK + + + + GA
Sbjct: 212 EDIAAPRCFEIEARLKKELNIPVFHDDQHGTAIVTLAALLNALKFVGKAMAAVRIVINGA 271
Query: 187 GAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIE 246
GAAG A +L ++G + + + K +V DL +++ E
Sbjct: 272 GAAGLAIAELLKESGATD-----IWICDSKGIVGKHRTDLNS--------KKQSFAVDAE 318
Query: 247 GGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARI 306
G +A+ GADV + + PGV+ E ++ M+ DPIVF +ANP+PEI P E + A +
Sbjct: 319 GTLADAMAGADVFLGVS--APGVVTKEMVQSMAKDPIVFAMANPIPEIQP-ELIQEDAAV 375
Query: 307 VATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPS--EENII 364
+ATGRSDYPNQINN+L FPG+FRGA+D RA IT M IEAAKAIAS+V + E+II
Sbjct: 376 IATGRSDYPNQINNVLAFPGVFRGAIDCRASIITTTMCIEAAKAIASLVHSNTLDSEHII 435
Query: 365 PSPFNPIVYAKEARAVAEEAMKEGVA 390
PS F+ V A AV A EGVA
Sbjct: 436 PSVFDNRVATTVASAVQLAARNEGVA 461
>pir||C72364 malate oxidoreductase - Thermotoga maritima (strain MSB8)
>gi|4981056|gb|AAD35627.1|AE001729_7 (AE001729) malate
oxidoreductase [Thermotoga maritima]
Length = 376
Score = 360 bits (914), Expect = 2e-98
Identities = 198/386 (51%), Positives = 260/386 (67%), Gaps = 12/386 (3%)
Query: 1 MDPLKFHK--GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSV 58
MD L+ H+ GKI V RE LSL YTPGVA+ +A A+DPEK Y YTS+ N+V
Sbjct: 1 MDALEIHRFLKGKIRTALPVEKVDRETLSLLYTPGVADVARACAEDPEKTYVYTSRWNTV 60
Query: 59 AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAIS 118
AVVSDGS +LGLGNIGP LPVMEGKA LFK F IDAFPI + E + +K I +VK++
Sbjct: 61 AVVSDGSAVLGLGNIGPYGALPVMEGKAFLFKAFADIDAFPICLSESEEEKIISIVKSLE 120
Query: 119 PTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDE 178
P+FGGINLEDI +PKCF IL +L EE++IPVFHDDQQGTA VV A +NAL++ K+++E
Sbjct: 121 PSFGGINLEDIGAPKCFRILQRLSEEMNIPVFHDDQQGTAVVVSAAFLNALKLTEKKIEE 180
Query: 179 ISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLE 238
+ V + G GAAG+ ++ L+ GVK NV V+ + +L ++ L Y +
Sbjct: 181 VKVVVNGIGAAGYNIVKFLLDLGVK--NVVAVD----RKGILNENDPETCLNEYHLEIAR 234
Query: 239 KTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDE 298
TN E + G E AL+GAD I +R ++KPEWI++MS P++F LANPVPEI P+
Sbjct: 235 ITNPERLSGDLETALEGADFFIGVSR--GNILKPEWIKKMSRKPVIFALANPVPEIDPEL 292
Query: 299 AKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
A++AGA IVATGRSD+PNQ+NNLL FPGI +GA++ R++ IT +M++ A +AIA EP
Sbjct: 293 AREAGAFIVATGRSDHPNQVNNLLAFPGIMKGAVEKRSK-ITKNMLLSAVEAIARSC-EP 350
Query: 359 SEENIIPSPFNPIVYAKEARAVAEEA 384
E IIP F+ V+ AV A
Sbjct: 351 EPERIIPEAFDMKVHLNVYTAVKGSA 376
>emb|CAC05958.1| (AL391763) putative malic enzyme [Streptomyces coelicolor]
Length = 409
Score = 355 bits (902), Expect = 5e-97
Identities = 197/386 (51%), Positives = 261/386 (67%), Gaps = 13/386 (3%)
Query: 7 HKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSR 66
H+GGK+ V + VP++ +E LSLAYTPGVA+ C AIA+ P+ V++YT K + VAVV+DG+
Sbjct: 35 HRGGKMAVQATVPIRDKEDLSLAYTPGVAKVCSAIAEQPDLVHDYTWKSSVVAVVTDGTA 94
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINL 126
+LGLG+IGP A LPVMEGKA+LFK+FGG+DA PI + D D+ +E V ++P+FGG+NL
Sbjct: 95 VLGLGDIGPEASLPVMEGKAILFKQFGGVDAVPIALNCTDADEIVETVVRLAPSFGGVNL 154
Query: 127 EDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGA 186
EDI++P+CF I KL+E LDIPVFHDDQ GTA V LA L NA + G+ L E+ + GA
Sbjct: 155 EDISAPRCFEIERKLQERLDIPVFHDDQHGTAVVTLAALRNAARLSGRTLGELRAVISGA 214
Query: 187 GAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIE 246
GAAG A ++LV+AG+ G+V V + K +V D E L P + L TN G+
Sbjct: 215 GAAGVAIAKMLVEAGI--GDVAVAD---RKGVV---SADREDLTPVKQELASFTNKAGLS 266
Query: 247 GGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARI 306
G E AL GADV I + G + + M++ VF +ANP PE+ P+ A K A +
Sbjct: 267 GPLEAALAGADVFIGVS---GGTVPEPAVASMAEGAFVFAMANPNPEVHPEVAHKYAA-V 322
Query: 307 VATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV-EEPSEENIIP 365
VATGRSD+PNQINN+L FPGIF GAL VRA IT+ M + AA+A+ASVV ++ + + +IP
Sbjct: 323 VATGRSDFPNQINNVLAFPGIFAGALQVRASRITEGMKLAAAEALASVVGDDLAADYVIP 382
Query: 366 SPFNPIVYAKEARAVAEEAMKEGVAR 391
SPF+ V AVA A EGVAR
Sbjct: 383 SPFDERVAPAVTAAVAAAARAEGVAR 408
>gb|AAF43042.1|AF234166_1 (AF234166) malic enzyme [Entamoeba histolytica]
Length = 487
Score = 349 bits (887), Expect = 3e-95
Identities = 199/400 (49%), Positives = 259/400 (64%), Gaps = 19/400 (4%)
Query: 5 KFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDG 64
KF+KG KI ++ K L + YTPGV+ I + + Y+ +GN V VVSD
Sbjct: 41 KFYKG-KIQIMPKCTLAGFNWFNAYYTPGVSRISTNIRDNNDSSLFYSLRGNFVGVVSDS 99
Query: 65 SRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMI------GEQDPDKFIEVVKAIS 118
+R+LG G++ P GL VMEGKALL K GGIDA PI I G+ DPD IE V+ I
Sbjct: 100 TRVLGDGDVTPPGGLGVMEGKALLMKYLGGIDAVPICIDSKNKEGKNDPDAVIEFVQRIQ 159
Query: 119 PTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDE 178
TFG INLEDI+ P C+ ILD LRE DIPV+HDDQQGTA+V LAGL+NAL++V K + E
Sbjct: 160 HTFGAINLEDISQPNCYKILDVLRESCDIPVWHDDQQGTASVTLAGLLNALKLVKKDIHE 219
Query: 179 ISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----LVNGKPMVLTKDLDLEKLFPYRG 234
+ GAG++ LR++V AG P + + + L NG+ +D+ + F YR
Sbjct: 220 CRMVFIGAGSSNTTCLRLIVTAGADPKKIVMFDSKGSLHNGR-----EDIKKDTRF-YRK 273
Query: 235 WLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEI 294
W + +T + G EA GADVLIS + PGPGV+K EWI+ M + PIVF ANPVPEI
Sbjct: 274 WEICETTNPSKFGSIAEACVGADVLISLSTPGPGVVKAEWIKSMGEKPIVFCCANPVPEI 333
Query: 295 LPDEAKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASV 354
P EAK+AGA IVATGR D+PNQ+NN +GFPGI +GAL VRAR ITD+M I A++A+A
Sbjct: 334 YPYEAKEAGAYIVATGRGDFPNQVNNSVGFPGILKGALIVRARKITDNMAIAASRALAEF 393
Query: 355 VEEP--SEENIIPSPFNPIVYAKEARAVAEEAMKEGVARV 392
E+ + +NII + P ++ KEA VA +A+K+GVARV
Sbjct: 394 AEKRGINPDNIIGTMDEPGIFPKEAADVAMQAIKDGVARV 433
>pir||H81336 malate dehydrogenase (oxaloacetate-decarboxylating) (EC 1.1.1.38)
Cj1287c [imported] - Campylobacter jejuni (strain NCTC
11168) >gi|6968719|emb|CAB73540.1| (AL139077) malate
oxidoreductase [Campylobacter jejuni]
Length = 411
Score = 341 bits (866), Expect = 8e-93
Identities = 196/421 (46%), Positives = 263/421 (61%), Gaps = 32/421 (7%)
Query: 2 DPLKFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVV 61
+ LK+H GGKI ++ P+ T LSLAY+PGVAEPC IAKD E Y YT+K N VA+V
Sbjct: 6 EALKYHLGGKIDIVPSKPMATSYDLSLAYSPGVAEPCLEIAKDNELAYTYTNKANLVAIV 65
Query: 62 SDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTF 121
SDGS +LGLGNIG A PVMEGKA LFK+F ++A+ I I ++ + KA++PT
Sbjct: 66 SDGSAVLGLGNIGAQASKPVMEGKACLFKKFANVNAYDIEINVHSIEEIVNFCKALAPTV 125
Query: 122 GGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISV 181
GGINLEDIA+PKCF I + ++L IPV HDDQ GTA + AGL+NA+E+ GK+ +I V
Sbjct: 126 GGINLEDIAAPKCFEI-EAALQDLGIPVMHDDQHGTAIISTAGLMNAMEISGKKFKDIKV 184
Query: 182 ALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTN 241
+ GAGAAG A+ ++ GV+ + LV+ K ++ D L P + LE
Sbjct: 185 VVSGAGAAGIASAKMYRNLGVEN-----IILVDSKGVI---SKDRNDLTPQK---LEFAV 233
Query: 242 DEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILP-DEAK 300
D E +E LKGADV + + P ++ E + M+ DP++F LANP+PE++P D A+
Sbjct: 234 DSK-EKTLKEVLKGADVFLGLS--APKILDDEMVLSMAKDPVIFALANPIPEVMPEDVAR 290
Query: 301 KAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP-- 358
IV TGRSDYPNQINN+LGFP IFRGALDVRA IT++M + AAKA+A + + P
Sbjct: 291 LRKDAIVGTGRSDYPNQINNVLGFPFIFRGALDVRASKITENMKVAAAKALADLAKLPVS 350
Query: 359 --------------SEENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEEIEERTL 404
+ +IP PF+ V A + AVA A+K+GVA+VK E+ +L
Sbjct: 351 DAVKKAYNLSTLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKNFDEKAYFESL 410
Query: 405 K 405
K
Sbjct: 411 K 411
>sp|P76558|MAO2_ECOLI NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|7466372|pir||F65021
hypothetical protein b2463 - Escherichia coli (strain
K-12) >gi|1788806|gb|AAC75516.1| (AE000333) putative
multimodular enzyme [Escherichia coli K12]
Length = 759
Score = 339 bits (860), Expect = 4e-92
Identities = 195/413 (47%), Positives = 260/413 (62%), Gaps = 42/413 (10%)
Query: 4 LKFHK---GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
L FH+ GKI V PL T+ L+LAY+PGVA PC I KDP K Y+YT++GN VAV
Sbjct: 10 LDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAV 69
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
+S+G+ +LGLGNIG LAG PVMEGK +LFK+F GID F I + E DPDKFIEVV A+ PT
Sbjct: 70 ISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPT 129
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGGINLEDI +P+CFYI KLRE ++IPVFHDDQ GTA + A ++N L VV K + ++
Sbjct: 130 FGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRVVEKNISDVR 189
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVE----LVNGKP--MVLTKDLDLEKLFPYRG 234
+ + GAGAA A + +LV G++ N+ V + + G+ M TK
Sbjct: 190 MVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETK------------ 237
Query: 235 WLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEI 294
D+G + ++ ++GAD+ + + GP V+ E +++M+ P++ LANP PEI
Sbjct: 238 -AAYAVVDDG-KRTLDDVIEGADIFLGCS--GPKVLTQEMVKKMARAPMILALANPEPEI 293
Query: 295 LPDEAKKAGA-RIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS 353
LP AK+ I+ TGRSDYPNQ+NN+L FP IFRGALDV A +I ++M + A +AIA
Sbjct: 294 LPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAE 353
Query: 354 VVE-EPSE---------------ENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
+ E SE E IIP PF+P + K A AVA+ AM+ GVA
Sbjct: 354 LAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMESGVA 406
>dbj|BAA16338.1| (D90875) PUTATIVE MALATE OXIDOREDUCTASE (NAD) (EC 1.1.1.38) (MALIC
ENZYME). [Escherichia coli]
Length = 490
Score = 339 bits (860), Expect = 4e-92
Identities = 195/413 (47%), Positives = 260/413 (62%), Gaps = 42/413 (10%)
Query: 4 LKFHK---GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
L FH+ GKI V PL T+ L+LAY+PGVA PC I KDP K Y+YT++GN VAV
Sbjct: 10 LDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAV 69
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
+S+G+ +LGLGNIG LAG PVMEGK +LFK+F GID F I + E DPDKFIEVV A+ PT
Sbjct: 70 ISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPT 129
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGGINLEDI +P+CFYI KLRE ++IPVFHDDQ GTA + A ++N L VV K + ++
Sbjct: 130 FGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRVVEKNISDVR 189
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVE----LVNGKP--MVLTKDLDLEKLFPYRG 234
+ + GAGAA A + +LV G++ N+ V + + G+ M TK
Sbjct: 190 MVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETK------------ 237
Query: 235 WLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEI 294
D+G + ++ ++GAD+ + + GP V+ E +++M+ P++ LANP PEI
Sbjct: 238 -AAYAVVDDG-KRTLDDVIEGADIFLGCS--GPKVLTQEMVKKMARAPMILALANPEPEI 293
Query: 295 LPDEAKKAGA-RIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS 353
LP AK+ I+ TGRSDYPNQ+NN+L FP IFRGALDV A +I ++M + A +AIA
Sbjct: 294 LPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAE 353
Query: 354 VVE-EPSE---------------ENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
+ E SE E IIP PF+P + K A AVA+ AM+ GVA
Sbjct: 354 LAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMESGVA 406
>pir||H82737 malate oxidoreductase XF0977 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9105909|gb|AAF83787.1|AE003936_1
(AE003936) malate oxidoreductase [Xylella fastidiosa]
Length = 764
Score = 334 bits (847), Expect = 1e-90
Identities = 179/398 (44%), Positives = 257/398 (63%), Gaps = 30/398 (7%)
Query: 10 GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILG 69
GKI V++ P+ T+ LSLAY+PGVA C+AI ++P++ E T++GN VAV+S+G+ +LG
Sbjct: 20 GKIKVVATKPMLTQRDLSLAYSPGVAFACQAIVENPQQASELTARGNLVAVISNGTAVLG 79
Query: 70 LGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLEDI 129
LGNIGPLAG PVMEGK +LF++F GID F I I E DPDK ++++ ++ PTFGG+NLEDI
Sbjct: 80 LGNIGPLAGKPVMEGKGVLFQKFAGIDVFDIEIDESDPDKLVDIIASLEPTFGGVNLEDI 139
Query: 130 ASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAA 189
+P+CF + KLRE + IPVFHDDQ GTA +V A ++NA+ + GK++ E+ +A G GAA
Sbjct: 140 KAPECFIVERKLRERMSIPVFHDDQHGTAIIVGAAVLNAMVITGKKIGEVKLATTGMGAA 199
Query: 190 GFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGP 249
G + + +LV G+ P N+ + G DLD EK ++ + +
Sbjct: 200 GVSCVNMLVSLGLNPDNILAYDR-EGVIHTGRTDLDPEK---------QRYARDTDKRTL 249
Query: 250 EEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDE--AKKAGARIV 307
E ++GAD+ + + G++ PE + M+ DP++F LANP+PEI+P+ A + A I+
Sbjct: 250 AEIVEGADIFLGLS--AAGILTPEMVATMAPDPVIFALANPIPEIMPEAVCAVRTDA-II 306
Query: 308 ATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV------------ 355
TGRSDYPNQINN+L FP +FRGALDV A +I ++M I KAIA++
Sbjct: 307 GTGRSDYPNQINNVLCFPYLFRGALDVGATTINEEMKIACVKAIAALARCEASDLGAAYG 366
Query: 356 -EEPS--EENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
E PS E +IP P +P + + + AVA+ AM GVA
Sbjct: 367 GETPSFGREYLIPRPLDPRLLVELSSAVAQAAMDSGVA 404
>pir||E83014 malic enzyme PA5046 [imported] - Pseudomonas aeruginosa (strain
PAO1) >gi|9951337|gb|AAG08431.1|AE004918_1 (AE004918)
malic enzyme [Pseudomonas aeruginosa]
Length = 422
Score = 333 bits (844), Expect = 3e-90
Identities = 188/407 (46%), Positives = 255/407 (62%), Gaps = 32/407 (7%)
Query: 4 LKFH---KGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
L++H + GK+ V P T LSLAY+PGVAEP + IA+D E Y+YT KGN VAV
Sbjct: 9 LEYHAQPRPGKLSVELTKPTATARDLSLAYSPGVAEPVREIARDAELAYQYTGKGNLVAV 68
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
+SDG+ ILGLGN+GPLA PVMEGK +LFKRF GID F I + + P FI+ VK IS T
Sbjct: 69 ISDGTAILGLGNLGPLASKPVMEGKGVLFKRFAGIDVFDIEVDAESPQAFIDTVKRISIT 128
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGGINLEDI +P+CF I L E+ DIPVFHDDQ GTA V AG++NALE+ GK+L+E
Sbjct: 129 FGGINLEDIKAPECFEIERALIEQCDIPVFHDDQHGTAIVTAAGMLNALELAGKKLEEAK 188
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
+ GAGAA + +++LV G K N + +++ K ++ DL + Y+ +T
Sbjct: 189 IVCLGAGAAAISCMKLLVSMGAKVEN---IYMIDRKGVIHAGRDDLNQ---YKSVFATET 242
Query: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
+ + +AL+GADV + + G +++PE ++ M+ +PIVF +NP PEI P+ A
Sbjct: 243 DKRTL----ADALEGADVFVGLS--GANLLEPEDLKRMAPNPIVFACSNPDPEIKPELAH 296
Query: 301 KA-GARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP- 358
I+ATGRSDYPNQ+NN+LGFP IFRGALDVRA I ++M I AA A+ + ++P
Sbjct: 297 ATRNDVIMATGRSDYPNQVNNVLGFPFIFRGALDVRATRINEEMKIAAALALRDLAKQPV 356
Query: 359 ---------------SEENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
E IIP P + + + AVA+ A++ GVA
Sbjct: 357 PEDVCAAYGVDKLEFGREYIIPKPMDKRLITVVSDAVAKAAIETGVA 403
>sp|P43837|MAO2_HAEIN NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|1075000|pir||F64112
malate dehydrogenase (oxaloacetate-decarboxylating)
homolog - Haemophilus influenzae (strain Rd KW20)
>gi|1574176|gb|AAC22896.1| (U32804) malate
oxidoreductase, putative [Haemophilus influenzae Rd]
Length = 756
Score = 328 bits (832), Expect = 7e-89
Identities = 182/407 (44%), Positives = 255/407 (61%), Gaps = 30/407 (7%)
Query: 4 LKFHK---GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
L FH+ GKI V L T+ L+LAY+PGVAEPC I KDP Y+YT++GN VAV
Sbjct: 10 LDFHEFPIPGKIEVTPTKSLATQRDLALAYSPGVAEPCLEIEKDPAASYKYTARGNLVAV 69
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
+S+G+ +LGLGNIG LAG PVMEGK +LFK+F GI+ F I + E DPDK ++++ ++ PT
Sbjct: 70 ISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGINVFDIEVNEHDPDKLVDIIASLEPT 129
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGG+NLEDI +P+CFYI KLRE ++IPVFHDDQ GTA + A +IN+L +VGK+++++
Sbjct: 130 FGGVNLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISAAAIINSLRIVGKKIEDVR 189
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
+ GAGAA A L +L+ G+K N+ V + V+ K D + + + +E
Sbjct: 190 LVASGAGAASIACLNLLLSLGMKRENITVCD----SKGVVYKGRDDKMDQTKKEYAIEDN 245
Query: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
+ +A+ AD+ + + G + + ++ M+ PI+ LANP PEI P EAK
Sbjct: 246 GWRKL----ADAIPNADIFLGCS--AAGALTQDMVKSMAAHPIILALANPNPEITPPEAK 299
Query: 301 KAGA-RIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASV-VEEP 358
IV TGRSDYPNQ+NN+L FP IFRGALDV A +I ++M A AIA + +EE
Sbjct: 300 AVRPDAIVCTGRSDYPNQVNNVLCFPFIFRGALDVGATTINEEMKRAAVYAIADLALEEQ 359
Query: 359 SE---------------ENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
+E + +IP PF+P + + A AVA+ AM+ GVA
Sbjct: 360 NEVVTSAYGGEGATFGADYVIPRPFDPRLIVRIAPAVAKAAMESGVA 406
>gb|AAF37577.1|AF234535_1 (AF234535) malic enzyme [Corynebacterium melassecola]
Length = 392
Score = 326 bits (828), Expect = 2e-88
Identities = 175/367 (47%), Positives = 243/367 (65%), Gaps = 13/367 (3%)
Query: 7 HKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSR 66
H+GGK+ + S PL+ LSLAYTPGVA+ C+AI +DPE +T GN+VAV+SDG+
Sbjct: 21 HEGGKLSISSTRPLRDMRDLSLAYTPGVAQVCEAIKEDPEVARTHTGIGNTVAVISDGTA 80
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINL 126
+LGLG+IGP A LPVMEGKA LF F G+ A PI++ D D +E + AI+P+FG INL
Sbjct: 81 VLGLGDIGPQASLPVMEGKAQLFSSFAGLKAIPIVLDVHDVDALVETIAAIAPSFGAINL 140
Query: 127 EDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGA 186
EDI++P+CF + +L E LDIPV HDDQ GTA V+LA L N+L+++ ++++++ + + GA
Sbjct: 141 EDISAPRCFEVERRLIERLDIPVMHDDQHGTAVVILAALRNSLKLLDRKIEDLKIVISGA 200
Query: 187 GAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIE 246
GAAG A + +L AG + +++ + ++ DL P + L EKTN GI
Sbjct: 201 GAAGVAAVDMLTNAGATD-----IVVLDSRGIIHDSREDLS---PVKDALAEKTNPRGIS 252
Query: 247 GGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARI 306
GG EA GAD+ I + G I + ++ M+ PI+F LANP PEI P+ ++K GA I
Sbjct: 253 GGINEAFTGADLFIGVS---GGNIGEDALKLMAPQPILFTLANPTPEIDPELSQKYGA-I 308
Query: 307 VATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE-ENIIP 365
VATGRSD PNQINN+L FPGIF GAL +A+ IT +M + AA+AIA + E E I+P
Sbjct: 309 VATGRSDLPNQINNVLAFPGIFAGALAAKAKKITPEMKLAAAEAIADIAAEDLEVGRIVP 368
Query: 366 SPFNPIV 372
+ +P V
Sbjct: 369 TALDPRV 375
>pir||D82047 probable malate oxidoreductase VC2681 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9657275|gb|AAF95822.1|
(AE004333) malate oxidoreductase, putative [Vibrio
cholerae]
Length = 425
Score = 326 bits (826), Expect = 4e-88
Identities = 179/409 (43%), Positives = 256/409 (61%), Gaps = 29/409 (7%)
Query: 1 MDPLKFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
+D + GKIG+ P + L+LAY+PGVAEP + IA++ + VY+YT+KGN+VAV
Sbjct: 20 LDYHAYPTAGKIGIALTKPADSAADLALAYSPGVAEPVREIAQNVDNVYKYTAKGNTVAV 79
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
+S+G+ ILGLGN+GP+A PVMEGKALLFKRF G+D+ I + + D+FI+ V I+ T
Sbjct: 80 ISNGTAILGLGNLGPMASKPVMEGKALLFKRFAGLDSIDIEVKHRTIDEFIDTVANIADT 139
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGGINLEDI +P CF I +L E D+PVFHDDQ GTA V AG++NALE+ GK+L +
Sbjct: 140 FGGINLEDIKAPDCFEIERRLIERCDVPVFHDDQHGTAIVTAAGMLNALELQGKQLKDCI 199
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
+ GAGAA A + +L+K G + +++ K ++ T+ DL + Y+ T
Sbjct: 200 IVCLGAGAAAVACMELLIKCGAMR---EKIYMLDRKGVIHTRRDDLNE---YKALFANNT 253
Query: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
+ + E+ + GAD+ + + GP ++ + ++ M+D P+VF +NP PEI P+ A
Sbjct: 254 DKRTL----EDVIAGADLFLGVS--GPNLLPADALKLMADKPVVFACSNPDPEIKPEIAH 307
Query: 301 KA-GARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP- 358
+ I+ TGRSDYPNQ+NN+L FP IFRGALDVRA I D M + A +AI + +EP
Sbjct: 308 QVRNDMIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASEINDQMKLAAVEAIRQLAKEPV 367
Query: 359 ---------------SEENIIPSPFNPIVYAKEARAVAEEAMKEGVARV 392
+ IIP P +P + + ARAVAE A++ GVAR+
Sbjct: 368 PAEVLAAAGVDKLSFGIDYIIPKPMDPRLLPRVARAVAEAAVESGVARI 416
>gb|AAG19885.1| (AE005072) malate dehydrogenase; Mdh [Halobacterium sp. NRC-1]
Length = 750
Score = 326 bits (826), Expect = 4e-88
Identities = 188/426 (44%), Positives = 258/426 (60%), Gaps = 37/426 (8%)
Query: 2 DPLKFHKG---GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSV 58
D L +H GK+ + + P T+ LSLAY+PGVA PC+AIA DP + + +T+KGN V
Sbjct: 6 DALDYHASDPPGKVEISTTKPTNTQRDLSLAYSPGVAAPCRAIADDPTEAFTHTTKGNMV 65
Query: 59 AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAIS 118
VVS+GS +LGLG+IG A PVMEGK +LFKRF ID F I + + D+ + V A+
Sbjct: 66 GVVSNGSAVLGLGDIGAQAAKPVMEGKGVLFKRFADIDVFDIELDQATADELVATVSAME 125
Query: 119 PTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDE 178
PTFGGINLEDI +P+CF + +L + +D+PVFHDDQ GTA + A L+NA E+ GK L +
Sbjct: 126 PTFGGINLEDIKAPECFEVERRLGDAMDVPVFHDDQHGTAIISGAALLNAAEIAGKDLSD 185
Query: 179 ISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLE 238
+ V GAGA+ AT + V GV ++ + + V+T+D D+ +
Sbjct: 186 LDVVFSGAGASAIATAKFYVSLGVDREHITMCD----SSGVITEDRDVNEFKA----AFA 237
Query: 239 KTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDE 298
+ D G G +A+ GADV + + G++ +R M+DDPIVF +ANP PEI +
Sbjct: 238 QPADSG--GDLADAMAGADVFVGLS--VGGIVNQAMVRSMADDPIVFAMANPDPEIDYES 293
Query: 299 AKKA--GARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVE 356
AK A I+ATGRSDYPNQ+NN+LGFP IFRGALDVRA I ++M + AA A+A + +
Sbjct: 294 AKAAREDTVIMATGRSDYPNQVNNVLGFPFIFRGALDVRATDINEEMKVAAATALADLAK 353
Query: 357 E--PSE--------------ENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVK----G 396
+ P E E IIP P +P V + A AVA+ AM+ GVAR ++
Sbjct: 354 QDVPDEVVKAYGDQPLQFGSEYIIPKPLDPRVLFEVAPAVADAAMETGVARTELDLVDYR 413
Query: 397 EEIEER 402
EE+E R
Sbjct: 414 EELEAR 419
>pir||T00127 hypothetical protein 5 - Leptospira interrogans
>gi|1902828|dbj|BAA19451.1| (AB001721) ORF5 [Leptospira
interrogans] >gi|2780771|dbj|BAA24374.1| (AB010203)
ORF5; putative [Leptospira interrogans]
Length = 437
Score = 324 bits (823), Expect = 8e-88
Identities = 186/410 (45%), Positives = 255/410 (61%), Gaps = 31/410 (7%)
Query: 7 HKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSR 66
H GKI V+ P + LSLAY+PGVA PC I PE VYEYT+KGN V ++++G+
Sbjct: 29 HPKGKIEVVPTKPTEDSYDLSLAYSPGVAYPCLEIKDSPELVYEYTNKGNLVGIITNGTA 88
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINL 126
ILGLG+IG LAG PVMEGKA+LFK+F GID F I I +DPD+FI VK + PTFGGINL
Sbjct: 89 ILGLGDIGALAGKPVMEGKAVLFKKFAGIDVFDIEINTKDPDEFIRAVKLLEPTFGGINL 148
Query: 127 EDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGA 186
EDI +P+ FYI ++L E+++IPVFHDDQ GTA + +AGL+N+ ++ GK D++ V + GA
Sbjct: 149 EDIKAPESFYIEEQLIEKMNIPVFHDDQHGTAIITVAGLLNSFKLTGKNPDDVKVVICGA 208
Query: 187 GAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIE 246
GAAG A ++ G+K + LV+ K ++ DL + ++KT+ +
Sbjct: 209 GAAGIAIAELIQHIGIKK---EQIFLVDTKGVIHHNRNDLN---DSKKKYIQKTDSTTL- 261
Query: 247 GGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKA--GA 304
+ +KG+D+ I + ++ + +R MS +P++F LANP PEI P KA
Sbjct: 262 ---RDVMKGSDIFIGVS--VENMVDEDMVRSMSKNPVIFALANPDPEI-PYRVAKAVRSD 315
Query: 305 RIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASV--VEEP---- 358
I+ TGRSD PNQ+NN+LGFP IFRGALDVRAR IT +M + AA+++A + +E P
Sbjct: 316 LIMGTGRSDNPNQVNNVLGFPFIFRGALDVRARHITLEMKLAAARSLAELARLEVPEVVS 375
Query: 359 ----------SEENIIPSPFNPIVYAKEARAVAEEAMKEGVARVKVKGEE 398
E +IP PF+ V A AVAE A+ G AR+ G E
Sbjct: 376 KAYGGEKFTFGPEYLIPKPFDKRVLFHVAPAVAEAAVASGSARIPYLGRE 425
>pir||C81173 malate oxidoreductase (NAD) NMB0671 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7225897|gb|AAF41089.1| (AE002421) malate
oxidoreductase (NAD) [Neisseria meningitidis MC58]
Length = 426
Score = 323 bits (819), Expect = 2e-87
Identities = 179/406 (44%), Positives = 256/406 (62%), Gaps = 27/406 (6%)
Query: 4 LKFHK---GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
LKFH+ GKI V L T + L+LAY+PGVA PC I DP+ Y+YT+KGN VAV
Sbjct: 10 LKFHEFPVPGKISVTPTKSLATDKDLALAYSPGVAAPCMEIHADPQNAYKYTAKGNLVAV 69
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
+S+G+ +LGLG+IG LAG PVMEGK +LFK+F G+D F I I E+DP K ++++ A+ PT
Sbjct: 70 ISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFAGVDVFDIEIDEKDPQKLVDIIAALEPT 129
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGGINLEDI +P+CFYI +LR+ IPVFHDDQ GTA + A ++NAL G++++E +
Sbjct: 130 FGGINLEDIKAPECFYIERELRKRCKIPVFHDDQHGTAIITAAAVLNALRFTGRKIEEAT 189
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
+ GAGAA A L L+ G+K NV V + K ++ D +++ + + +
Sbjct: 190 LVCSGAGAAAIACLNQLLDLGLKRENVTVCD---SKGVIYQTREDKDRMDESKQFYAIED 246
Query: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
N + + +A+KG D+ + + G ++ PE + M++ PIVF +ANP PEILP AK
Sbjct: 247 NGQRVLA---DAVKGKDIFLGLS--GANLLTPEILNTMNEKPIVFAMANPNPEILPPLAK 301
Query: 301 KAGARIV-ATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASV-VEEP 358
+ +V TGRSD+PNQ+NN+L FP IFRGALDV A +I ++M A+A + +EE
Sbjct: 302 ETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGALDVGATTINEEMKRACVYALADLAMEEV 361
Query: 359 SE--------------ENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
+E E +IP+PF+ + + A A A+ AM+ GVA
Sbjct: 362 TEEVVAAYGKKFEFGAEYLIPTPFDSRLLPRVATAAAKAAMESGVA 407
>pir||A81933 probable malate dehydrogenase (oxaloacetate-decarboxylating) (EC
1.1.1.38) NMA0870 [imported] - Neisseria meningitidis
(group A strain Z2491) >gi|7379585|emb|CAB84150.1|
(AL162754) putative malate oxidoreductase [Neisseria
meningitidis Z2491]
Length = 426
Score = 323 bits (819), Expect = 2e-87
Identities = 179/406 (44%), Positives = 256/406 (62%), Gaps = 27/406 (6%)
Query: 4 LKFHK---GGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAV 60
LKFH+ GKI V L T + L+LAY+PGVA PC I DP+ Y+YT+KGN VAV
Sbjct: 10 LKFHELPVPGKISVTPTKSLATDKDLALAYSPGVAAPCMEIHADPQNAYKYTAKGNLVAV 69
Query: 61 VSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPT 120
+S+G+ +LGLG+IG LAG PVMEGK +LFK+F G+D F I I E+DP K ++++ A+ PT
Sbjct: 70 ISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFAGVDVFDIEIDEKDPQKLVDIIAALEPT 129
Query: 121 FGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS 180
FGGINLEDI +P+CFYI +LR+ IPVFHDDQ GTA + A ++NAL G++++E +
Sbjct: 130 FGGINLEDIKAPECFYIERELRKRCKIPVFHDDQHGTAIITAAAVLNALRFTGRKIEEAT 189
Query: 181 VALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
+ GAGAA A L L+ G+K NV V + K ++ D +++ + + +
Sbjct: 190 LVCSGAGAAAIACLNQLLDLGLKRENVTVCD---SKGVIYQTREDKDRMDESKQFYAIED 246
Query: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
N + + +A+KG D+ + + G ++ PE + M++ PIVF +ANP PEILP AK
Sbjct: 247 NGQRVLA---DAVKGKDIFLGLS--GANLLTPEILNTMNEKPIVFAMANPNPEILPPLAK 301
Query: 301 KAGARIV-ATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASV-VEEP 358
+ +V TGRSD+PNQ+NN+L FP IFRGALDV A +I ++M A+A + +EE
Sbjct: 302 ETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGALDVGATTINEEMKRACVYALADLAMEEV 361
Query: 359 SE--------------ENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
+E E +IP+PF+ + + A A A+ AM+ GVA
Sbjct: 362 TEEVVAAYGKKFEFGAEYLIPTPFDSRLLPRVATAAAKAAMESGVA 407
>sp|O30808|MAO2_RHIME NADP-DEPENDENT MALIC ENZYME (NADP-ME)
Length = 761
Score = 319 bits (808), Expect = 5e-86
Identities = 188/441 (42%), Positives = 262/441 (58%), Gaps = 36/441 (8%)
Query: 2 DPLKFH---KGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSV 58
+ L FH + GK+ + P+ T+ LSLAY+PGVA P KAIA DP Y+YT++GN V
Sbjct: 18 EALDFHSQGRPGKLEISPTKPMATQRDLSLAYSPGVAVPVKAIADDPATAYDYTARGNMV 77
Query: 59 AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAIS 118
AV+S+G+ ILGLGN+G LA PVMEGKA+LFKRF +D+ + + ++ D+F+ V+ +
Sbjct: 78 AVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVDTENVDEFVNCVRFLG 137
Query: 119 PTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDE 178
P+FGGINLEDI +P CF I +LRE +DIPVFHDDQ GTA + AGL+NAL + G+
Sbjct: 138 PSFGGINLEDIKAPDCFIIEQRLREVMDIPVFHDDQHGTAIIAAAGLVNALTLTGRDFKT 197
Query: 179 ISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLE 238
+ GAGAA A + ++ G P N + L + K ++ D + ++
Sbjct: 198 AKLVCNGAGAAAIACIELIKAMGFNPEN---IILCDTKGVIYKGRTD--GMNQWKSAHAV 252
Query: 239 KTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDE 298
+T+ + EAL GADV F G + + +R M PI+F +ANP PEI P+E
Sbjct: 253 ETDRRTL----AEALDGADVF--FGLSAKGALSADMVRSMGARPIIFAMANPDPEITPEE 306
Query: 299 -AKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEE 357
A IVATGRSDYPNQ+NN+LGFP IFRGALDVRA +I D M I AA+A+A++ +E
Sbjct: 307 VALIRDDAIVATGRSDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAAEALANLAKE 366
Query: 358 PSEEN----------------IIPSPFNPIVYAKEARAVAEEAMKEGVAR-----VKVKG 396
++ IIP PF+P + + AVA+ AM+ GVAR +K G
Sbjct: 367 DVPDDVAAAYQGNRPRFGPQYIIPVPFDPRLISAIPMAVAKAAMETGVARKPIEDLKAYG 426
Query: 397 EEIEERTLKLISLWDDILGEV 417
+++ R + S I+ V
Sbjct: 427 QQLSARRDPIASTLQRIVERV 447
>gb|AAB82460.1| (AF017444) NADP-dependent malic enzyme; TME [Sinorhizobium
meliloti]
Length = 764
Score = 317 bits (805), Expect = 1e-85
Identities = 187/441 (42%), Positives = 261/441 (58%), Gaps = 36/441 (8%)
Query: 2 DPLKFH---KGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSV 58
+ L FH + GK+ + P+ T+ LSLAY+PGVA P KAIA DP Y+YT++GN V
Sbjct: 18 EALDFHSQGRPGKLEISPTKPMATQRDLSLAYSPGVAVPVKAIADDPATAYDYTARGNMV 77
Query: 59 AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAIS 118
AV+S+G+ ILGLGN+G LA PVMEGKA+LFKRF +D+ + + ++ D+F+ V+ +
Sbjct: 78 AVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVDTENVDEFVNCVRFLG 137
Query: 119 PTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDE 178
P+FGGINLEDI +P CF I +LRE +DIPVFHDDQ GTA + AGL+NAL + G+
Sbjct: 138 PSFGGINLEDIKAPDCFIIEQRLREVMDIPVFHDDQHGTAIIAAAGLVNALTLTGRDFKT 197
Query: 179 ISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLE 238
+ GAGAA A + ++ G P N+ + K ++ D + ++
Sbjct: 198 AKLVCNGAGAAAIACIELIKAMGFNPENIIFCDT---KGVIYKGRTD--GMNQWKSAHAV 252
Query: 239 KTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDE 298
+T+ + EAL GADV F G + + +R M PI+F +ANP PEI P+E
Sbjct: 253 ETDRRTL----AEALDGADVF--FGLSAKGALSADMVRSMGARPIIFAMANPDPEITPEE 306
Query: 299 -AKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEE 357
A IVATGRSDYPNQ+NN+LGFP IFRGALDVRA +I D M I AA+A+A++ +E
Sbjct: 307 VALIRDDAIVATGRSDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAAEALANLAKE 366
Query: 358 PSEEN----------------IIPSPFNPIVYAKEARAVAEEAMKEGVAR-----VKVKG 396
++ IIP PF+P + + AVA+ AM+ GVAR +K G
Sbjct: 367 DVPDDVAAAYQGNRPRFGPQYIIPVPFDPRLISAIPMAVAKAAMETGVARKPIEDLKAYG 426
Query: 397 EEIEERTLKLISLWDDILGEV 417
+++ R + S I+ V
Sbjct: 427 QQLSARRDPIASTLQRIVERV 447
>sp|O30807|MAO1_RHIME NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|2582193|gb|AAB82459.1|
(AF017443) NAD-malic enzyme; diphosphopyridine
nucleotide (NAD+)-dependent-malic enzyme [Sinorhizobium
meliloti]
Length = 770
Score = 315 bits (798), Expect = 7e-85
Identities = 171/407 (42%), Positives = 257/407 (63%), Gaps = 36/407 (8%)
Query: 5 KFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDG 64
++ + GK+ + PL + L+LAY+PGVA PC AI +PE ++T++ N VAVVS+G
Sbjct: 27 RYPRPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIKDNPETAADFTARANLVAVVSNG 86
Query: 65 SRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGI 124
+ +LGLGNIGPLA PVMEGKA+LFK+F GID F I I D+ ++V+ A+ PTFGGI
Sbjct: 87 TAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPTVDRMVDVISALEPTFGGI 146
Query: 125 NLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALF 184
NLEDI +P+CF + +LRE+++IPVFHDDQ GTA +V A ++N LE+ GK + E +
Sbjct: 147 NLEDIKAPECFEVERRLREKMEIPVFHDDQHGTAIIVAAAVLNGLELAGKDIAEAKIVAS 206
Query: 185 GAGAAGFATLRILVKAGVKPGNVRVVEL----VNGKPMVLTKDLDLEKLFPYRGWLLEKT 240
GAGAA A L +LV G + N+ V ++ G+ ++ + ++ +++
Sbjct: 207 GAGAAALACLNLLVTLGARRENIWVHDIEGLVYKGREALMDE---------WKAVYAQES 257
Query: 241 NDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
++ + +++ GADV + + GV+KPE + M++ P++ LANP PEI+P+ A+
Sbjct: 258 DNRVL----ADSIGGADVFLGLS--AAGVLKPELLARMAEKPLIMALANPTPEIMPEVAR 311
Query: 301 KAGA-RIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV-EEP 358
A ++ TGRSD+PNQ+NN+L FP IFRGALD AR+I ++M + A +AIA + EEP
Sbjct: 312 AARPDAMICTGRSDFPNQVNNVLCFPHIFRGALDCGARTINEEMKMAAVRAIAGLAREEP 371
Query: 359 SE---------------ENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
S+ + +IPSPF+ + + A AVA+ A + GVA
Sbjct: 372 SDVAARAYSGETPVFGPDYLIPSPFDQRLILRIAPAVAKAAAESGVA 418
>sp|Q9ZDF6|MAO2_RICPR PROBABLE NADP-DEPENDENT MALIC ENZYME (NADP-ME)
>gi|7467886|pir||F71694 malic enzyme (tme) RP373 -
Rickettsia prowazekii >gi|3860932|emb|CAA14832.1|
(AJ235271) MALIC ENZYME (tme) [Rickettsia prowazekii]
Length = 767
Score = 313 bits (794), Expect = 2e-84
Identities = 180/416 (43%), Positives = 249/416 (59%), Gaps = 39/416 (9%)
Query: 2 DPLKFH---KGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSV 58
+ L++H K GKI + + L T++ LSLAY+PGVA PC I+K+ E VY+YTS+ N V
Sbjct: 11 EALEYHEKDKPGKIAITTTKSLVTQQDLSLAYSPGVAAPCLEISKNLEAVYKYTSRSNLV 70
Query: 59 AVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAIS 118
AV+S+G+ +LGLGN+G A PVMEGKA+LFK+F IDA + + +DP +FI VK +
Sbjct: 71 AVISNGTAVLGLGNLGAAASKPVMEGKAVLFKKFADIDAIDLEVNTEDPIEFINAVKYLG 130
Query: 119 PTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDE 178
+FGGINLEDI +P+CF I +KL+ +DIPVFHDDQ GTA + AGLINA + + L +
Sbjct: 131 YSFGGINLEDIKAPECFLIEEKLKSLMDIPVFHDDQHGTAIITAAGLINAAYLTNRTLKD 190
Query: 179 ISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----LVNGKPMVLTKDLDLEKLFPYRG 234
+ + + GAGAA A + +L+ GV + + + + G+ + K
Sbjct: 191 LKIVINGAGAAAIACIDLLIALGVDKSKIILCDTKGVIYKGRTSGMNK------------ 238
Query: 235 WLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEI 294
W +D I E+L ADV I + G + + I +M+ PI+F +ANP PEI
Sbjct: 239 WKERYASDTKIR-TLTESLNNADVFIGLS--VKGAVTKDMISKMAHKPIIFAMANPDPEI 295
Query: 295 LPDEAK-KAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS 353
P++ K I+ATGRSDY NQ+NN++GFP IFRGALDVRA +I +M I AA+AIA
Sbjct: 296 TPEDIKFVRDDAIIATGRSDYNNQVNNVMGFPYIFRGALDVRASTINTEMKIAAARAIAD 355
Query: 354 VVEEP----------------SEENIIPSPFNPIVYAKEARAVAEEAMKEGVARVK 393
+ P E IIP PF+P + A AVA A++ GVARVK
Sbjct: 356 LARRPVPEEVYKAYSGRKMVFGNEYIIPVPFDPRLITVVATAVAVAAIESGVARVK 411
>pir||T46727 probable malic enzyme [imported] - Weissella paramesenteroides
plasmid >gi|6249490|emb|CAB60039.1| (AJ132782) putative
malic enzyme [Weissella paramesenteroides]
Length = 378
Score = 294 bits (744), Expect = 2e-78
Identities = 161/380 (42%), Positives = 240/380 (62%), Gaps = 15/380 (3%)
Query: 4 LKFHKG--GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVV 61
LK HK G + + + + +KT + LS AYTPGV+ K IA+D YT G + ++
Sbjct: 5 LKVHKSHTGVLDIKADISIKTPDDLSEAYTPGVSGIAKMIAEDEALKNVYTMSGKLIPII 64
Query: 62 SDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTF 121
+DGS +LGLGNIGP AGLP++EGKAL++K F G++A P+ + + D D+F+E +K ++P+F
Sbjct: 65 TDGSAVLGLGNIGPAAGLPIVEGKALIYKEFSGVNAIPMALNQVDVDEFVETIKTMAPSF 124
Query: 122 GGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISV 181
GI+LEDIA+P+ F I +L EELDIPV+HDDQ GTA VVLA LINA +VV K L ++ V
Sbjct: 125 AGIHLEDIAAPRVFEIEKRLNEELDIPVYHDDQTGTAVVVLAALINAAKVVNKPLKDLKV 184
Query: 182 ALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTN 241
+ G GAAG AT +IL+ +G+K N+ +V++ ++ D D + Y+ L N
Sbjct: 185 VINGMGAAGVATAKILLASGMK--NLTLVDI---HGVIRADDNDYNE---YQRELATAVN 236
Query: 242 DEGIEG-GPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAK 300
++G ++ + DV I + V+ E + M+ D IVF LANPVPEILP+EA
Sbjct: 237 Q--VDGRSLDDVIDNQDVFIGLS--DANVLSKEQVTRMAQDAIVFALANPVPEILPEEAH 292
Query: 301 KAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE 360
+ GA I ATG S + NQ+NN+L FPG+F+G L + + + ++ A+ +A +V P+
Sbjct: 293 EGGATITATGSSQWANQVNNVLVFPGLFKGLLATDIKKVELSLQVKLAEGLAQMVTTPTA 352
Query: 361 ENIIPSPFNPIVYAKEARAV 380
++I+P F+ V + AV
Sbjct: 353 DHIVPRVFDDGVVDAVSNAV 372
>emb|CAA71638.1| (Y10621) malic enzyme [Leuconostoc mesenteroides]
Length = 378
Score = 293 bits (742), Expect = 3e-78
Identities = 156/363 (42%), Positives = 237/363 (64%), Gaps = 15/363 (4%)
Query: 10 GKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILG 69
G + + S + T LS AYTPGVA K IAKD K +YT G + V++DGS +LG
Sbjct: 16 GVLDIESCFDITTPTDLSEAYTPGVAGLAKLIAKDTSKKRDYTMSGKLIPVITDGSAVLG 75
Query: 70 LGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGGINLEDI 129
LGNIGP AGLP++EGKAL++K F +DA P+ + + D+F++ VKAI+P+F GI+LEDI
Sbjct: 76 LGNIGPAAGLPIVEGKALIYKNFANVDALPMALNQMSVDQFVDTVKAIAPSFAGIHLEDI 135
Query: 130 ASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAG-- 187
++PK F I +L ELDIPV+HDDQ GTA VVLAG++NA +VVGK + ++ + + G G
Sbjct: 136 SAPKVFEIERRLSNELDIPVYHDDQTGTAIVVLAGIMNAAKVVGKNVHDLKIVVNGIGRC 195
Query: 188 AAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEG 247
AG AT +IL+ AG+ N+ +V+ +NG ++ D + Y+ L+ + ++G
Sbjct: 196 TAGVATTKILIAAGI--CNITLVD-ING--VINLGDANSNS---YQQELVREVTQ--VDG 245
Query: 248 GP-EEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGARI 306
++ + DV I + V+ + + M+++PI+F LANPVPEI PD+AKKAGA +
Sbjct: 246 KTLDDVIANQDVFIGLS--DANVLSGKQVARMNENPIIFALANPVPEISPDDAKKAGAAV 303
Query: 307 VATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEENIIPS 366
+ATG S +PNQ+NN+L FPG+F+G L+ + + + + A A+A+++ EP +I+P+
Sbjct: 304 IATGSSQWPNQVNNVLVFPGLFKGLLESGIKHVDMALQLNVAHALANMISEPDATHIVPA 363
Query: 367 PFN 369
F+
Sbjct: 364 VFD 366
>sp|P54572|MAO1_BACSU PROBABLE MALATE OXIDOREDUCTASE [NAD] (MALIC ENZYME) (ME)
>gi|7431224|pir||C69967 malate dehydrogenase homolog
yqkJ - Bacillus subtilis >gi|1303990|dbj|BAA12645.1|
(D84432) YqkJ [Bacillus subtilis]
>gi|2634790|emb|CAB14287.1| (Z99116) similar to malate
dehydrogenase [Bacillus subtilis]
Length = 439
Score = 285 bits (722), Expect = 6e-76
Identities = 165/382 (43%), Positives = 226/382 (58%), Gaps = 32/382 (8%)
Query: 4 LKFHKGGKIGVISKVPLKTREHLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSD 63
L H+GGKI + SK+P+++ L YTPGVA+ C+ I K+PEK YT+ NSVA+V+D
Sbjct: 86 LSAHEGGKIQMKSKMPIRSLAELGRVYTPGVADVCRLIEKEPEKASIYTTISNSVAIVTD 145
Query: 64 GSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGEQDPDKFIEVVKAISPTFGG 123
G+ ILGLGNIG +AG+PVMEGKA LF + GI PI++ DP++ I+ VK ISP F G
Sbjct: 146 GTAILGLGNIGSVAGMPVMEGKAALFDQLAGISGIPILLDTSDPEEIIKTVKHISPGFSG 205
Query: 124 INLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVAL 183
I LEDI SP CF I D+L+EEL+IPV HDDQ GTA V LA I+A G L E V
Sbjct: 206 ILLEDIGSPHCFEIEDRLKEELNIPVMHDDQHGTAVVTLAAAISACRSAGVDLKEAKVGQ 265
Query: 184 FGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDE 243
G GAAG A R+ + G VN + + +L Y G +
Sbjct: 266 IGLGAAGVAICRMFMAYG-----------VNAVYGTDKSESAMNRLEQYGGQAVSSI--- 311
Query: 244 GIEGGPEEALKGADVLISFTRPGPGVIKPEWIREMSDDPIVFPLANPVPEILPDEAKKAG 303
EE ++ D++I+ T PG+IKP ++R ++ L+NP PEI P+ A +AG
Sbjct: 312 ------EELMETCDIVIA-TTGVPGLIKPAFVR---SGQVILALSNPKPEIEPEAALQAG 361
Query: 304 ARIVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEENI 363
A A GRS +NN+LGFPGIFRGAL+ ++ I DM++ AA+AIA+ + + ++
Sbjct: 362 AAYAADGRS-----VNNVLGFPGIFRGALNAKSTEINHDMLVAAAEAIAACTK---QGDV 413
Query: 364 IPSPFNPIVYAKEARAVAEEAM 385
+P P + V+ A AV A+
Sbjct: 414 VPQPLDSKVHHAVAAAVEHAAL 435
>dbj|BAA16337.1| (D90874) PUTATIVE MALATE OXIDOREDUCTASE (NAD) (EC 1.1.1.38) (MALIC
ENZYME). [Escherichia coli]
Length = 598
Score = 145 bits (363), Expect = 7e-34
Identities = 96/258 (37%), Positives = 143/258 (55%), Gaps = 33/258 (12%)
Query: 153 DQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVEL 212
DQ GTA + A ++N L VV K + ++ + + GAGAA A + +LV G++ N+ V +
Sbjct: 1 DQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCD- 59
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGP---EEALKGADVLISFTRPGPGV 269
+K + + P K ++ G ++ ++GAD+ + + GP V
Sbjct: 60 --------SKGVIYQGREP--NMAETKAAYAVVDDGKRTLDDVIEGADIFLGCS--GPKV 107
Query: 270 IKPEWIREMSDDPIVFPLANPVPEILPDEAKKAGA-RIVATGRSDYPNQINNLLGFPGIF 328
+ E +++M+ P++ LANP PEILP AK+ I+ TGRSDYPNQ+NN+L FP IF
Sbjct: 108 LTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIF 167
Query: 329 RGALDVRARSITDDMIIEAAKAIASVVE-EPSE---------------ENIIPSPFNPIV 372
RGALDV A +I ++M + A +AIA + E SE E IIP PF+P +
Sbjct: 168 RGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRL 227
Query: 373 YAKEARAVAEEAMKEGVA 390
K A AVA+ AM+ GVA
Sbjct: 228 IVKIAPAVAKAAMESGVA 245
>pir||T02763 probable malate dehydrogenase (oxaloacetate-decarboxylating)
(NADP+) (EC 1.1.1.40) - maize
>gi|2950395|emb|CAA12157.1| (AJ224847) oxidoreductase
[Zea mays]
Length = 652
Score = 118 bits (294), Expect = 9e-26
Identities = 132/442 (29%), Positives = 198/442 (43%), Gaps = 84/442 (19%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y +T K V VV+DG R
Sbjct: 194 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGRILEVLRNWTEKSIQVIVVTDGER 253
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 254 ILGLGDLG-CQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNEDLLKDEFYIGLKQR 312
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G + ED A+ F +L+K R + VF+DD QG
Sbjct: 313 RATGQEYSEFLDEFMAAVRQNYGQKVLVQFEDFANHNAFTLLEKYRT--NHLVFNDDIQG 370
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TAAVVLAG+I AL+ VG L + + FGAG AG ++ + NV V E
Sbjct: 371 TAAVVLAGIIAALKSVGGTLADHTFLFFGAGEAGTGIAELVALEISRQANVSVEEARKKI 430
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGG-----PEEALKGADVLISFTRP 265
LV+ K +++ + + F R + E + + G P + A V SFT+
Sbjct: 431 WLVDSKGLIVASREETLQPFKKR-YAHEHEPVKDLLGAVKAIRPTALIGSAGVGQSFTKE 489
Query: 266 GPGVIKPEWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVATG----------R 311
VI E + +++ PIV L+NP E ++A G I +G +
Sbjct: 490 ---VI--EVMSSINERPIVLALSNPTSQSECTAEQAYTWSQGRAIFGSGSPFDPVKYNDK 544
Query: 312 SDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNP 370
P Q NN FPG G + A ++DDM++ AA+A+A V E E+ +I PF+
Sbjct: 545 LFVPAQANNAYIFPGFGLGVVISGAVRVSDDMVLAAAEALAEQVTPEHIEKGLIYPPFSI 604
Query: 371 I--VYAKEARAVAEEAMKEGVA 390
I + A A VA +A G+A
Sbjct: 605 IRKISANIAAHVAAKAYDLGMA 626
>pir||D83211 probable malic enzyme PA3471 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9949615|gb|AAG06859.1|AE004768_4
(AE004768) probable malic enzyme [Pseudomonas
aeruginosa]
Length = 564
Score = 116 bits (288), Expect = 5e-25
Identities = 125/445 (28%), Positives = 199/445 (44%), Gaps = 83/445 (18%)
Query: 24 EHLSLAYTPGVAEPCKAIAK-------------DPEKVYEY----TSKGNSVAVVSDGSR 66
E + + YTP V + C+ +K D E++ + T + VV+D R
Sbjct: 96 EMMPIIYTPTVGQACQEFSKIYRTHRGLFISYPDRERIDDILRSATKNNVKIVVVTDSER 155
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIM--IGEQDPD------------- 108
ILGLG+ G + G+ + GK L+ GGI P++ +G +PD
Sbjct: 156 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPDLLNDPMYMGWRHE 214
Query: 109 --------KFIEV-VKAISPTFGGINL--EDIASPKCFYILDKLREELDIPVFHDDQQGT 157
+F+++ ++AI + + L ED A +L++ ++EL F+DD QGT
Sbjct: 215 RVSGAQYEEFVDLFIQAIKRRWPNVLLQFEDFAQTNAMPLLERYKDELCC--FNDDIQGT 272
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKA----GVKPGNVRVVELV 213
AAV + L+ A + G++L E +V GAG+AG ++ A G+ R +
Sbjct: 273 AAVAVGTLLAACKAKGEKLSEQTVTFVGAGSAGCGIAEQIIAAMQLEGLDEAQARRRIFM 332
Query: 214 NGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPE-------EALKGADVLISFTRPG 266
+ +LT D+ L ++ L +K D G GG + E ++ A + G
Sbjct: 333 VDRWGLLTD--DMSNLLDFQHRLAQKRADLGAWGGQQGDDLALLEVIRNARPTVLIGVSG 390
Query: 267 P-GVIKPEWIREMSD---DPIVFPLANPVP--EILPDEAKK--AGARIVATGRSDYPNQI 318
G+ E IRE+ P+V PL+NP E P E G +VATG P Q+
Sbjct: 391 QRGLFSEEVIRELHSHCKQPLVMPLSNPTSRVEATPQEILNWTDGQALVATGSPFQPVQV 450
Query: 319 ----------NNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA--SVVEEPSEENIIPS 366
NN FPGI G + RA +T+ M++ AA A+A S + E ++P+
Sbjct: 451 GDKRIPIAQCNNAYIFPGIGLGVIAARANRVTEGMLMAAANALANCSPIVTQGEGAVLPA 510
Query: 367 PFNPIVYAKE-ARAVAEEAMKEGVA 390
+ +K A AVA++A EG A
Sbjct: 511 LGDIREVSKRIAVAVAKQAQAEGKA 535
>emb|CAA56354.1| (X80051) NADP dependent malic enzyme [Phaseolus vulgaris]
Length = 589
Score = 115 bits (286), Expect = 8e-25
Identities = 124/441 (28%), Positives = 197/441 (44%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + K V VV+DG R
Sbjct: 131 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTDGER 190
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 191 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQR 249
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E ++A+ +G + ED A+ F +L+K VF+DD QG
Sbjct: 250 RATGQEYAELLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSH--LVFNDDIQG 307
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+ +L++VG L + + GAG AG ++ K V E
Sbjct: 308 TASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVEETRKKI 367
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKG--ADVLISFTRPGPG 268
LV+ K ++++ L+ + F + W E +G+ EA+K VLI + G
Sbjct: 368 WLVDSKGLIVSSRLESLQQFK-KPWAHEHEPVKGL----LEAVKAIKPTVLIGSSGAGKT 422
Query: 269 VIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
K E + +++ P++ L+NP E +EA G I A+ G+
Sbjct: 423 FTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKL 482
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNPI 371
P Q NN FPG G + A + D+M++ A++A+A+ V EE ++ +I PF I
Sbjct: 483 FVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTNI 542
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ A A VA +A G+A
Sbjct: 543 RKISANIAAKVAAKAYDLGLA 563
>pir||S48198 malic enzyme, NADP-dependent - kidney bean >gi|7427668|pir||DEFBC
malate dehydrogenase (oxaloacetate-decarboxylating)
(NADP+) (EC 1.1.1.40) - kidney bean
Length = 589
Score = 115 bits (286), Expect = 8e-25
Identities = 124/441 (28%), Positives = 197/441 (44%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + K V VV+DG R
Sbjct: 131 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTDGER 190
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 191 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQR 249
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E ++A+ +G + ED A+ F +L+K VF+DD QG
Sbjct: 250 RATGQEYAELLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSH--LVFNDDIQG 307
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+ +L++VG L + + GAG AG ++ K V E
Sbjct: 308 TASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVEETRKKI 367
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKG--ADVLISFTRPGPG 268
LV+ K ++++ L+ + F + W E +G+ EA+K VLI + G
Sbjct: 368 WLVDSKGLIVSSRLESLQQFK-KPWAHEHEPVKGL----LEAVKAIKPTVLIGSSGAGKT 422
Query: 269 VIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
K E + +++ P++ L+NP E +EA G I A+ G+
Sbjct: 423 FTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKL 482
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNPI 371
P Q NN FPG G + A + D+M++ A++A+A+ V EE ++ +I PF I
Sbjct: 483 FVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTNI 542
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ A A VA +A G+A
Sbjct: 543 RKISANIAAKVAAKAYDLGLA 563
>sp|P12628|MAOX_PHAVU NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|169327|gb|AAA19575.1|
(J03825) NADP-dependent malic enzyme [Phaseolus
vulgaris]
Length = 589
Score = 114 bits (282), Expect = 2e-24
Identities = 124/441 (28%), Positives = 196/441 (44%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + K V VV+DG R
Sbjct: 131 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTDGER 190
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 191 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQR 249
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ E ++A+ +G + ED A+ F +L+K VF+DD QG
Sbjct: 250 RATGQEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSH--LVFNDDIQG 307
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+ +L++VG L + + GAG AG ++ K V E
Sbjct: 308 TASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVEETRKKI 367
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKG--ADVLISFTRPGPG 268
LV+ K ++++ L+ + F + W E +G+ EA+K VLI + G
Sbjct: 368 WLVDSKGLIVSSRLESLQQFK-KPWAHEHEPVKGL----LEAVKAIKPTVLIGSSGAGKT 422
Query: 269 VIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
K E + +++ P++ L+NP E +EA G I A+ G+
Sbjct: 423 FTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKL 482
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNPI 371
P Q NN FPG G + A + D+M++ A++A+A+ V EE ++ +I PF I
Sbjct: 483 FVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTNI 542
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ A A VA +A G+A
Sbjct: 543 RKISANIAAKVAAKAYDLGLA 563
>sp|P16243|MAOC_MAIZE NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PRECURSOR (NADP-ME)
>gi|65939|pir||DEZMMX malate dehydrogenase
(oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40)
precursor, chloroplast - maize >gi|168528|gb|AAA33487.1|
(J05130) NADP-dependent malic enzyme (EC 1.1.1.40) [Zea
mays]
Length = 636
Score = 113 bits (280), Expect = 4e-24
Identities = 131/443 (29%), Positives = 197/443 (43%), Gaps = 86/443 (19%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L YTP V E C+ KD KV E + + V V+DG R
Sbjct: 178 ELLPFVYTPTVGEACQKYGSIFGRPQGLYVSLKDKGKVLEVLRNWPHRNIQVICVTDGER 237
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+D PI I
Sbjct: 238 ILGLGDLG-CQGMGIPVGKLALYTALGGVDPSVCLPITIDVGTNNEFLLNDEFYIGLRQK 296
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
GE+ + E + A+ +G I ED A+ F +L+K + VF+DD QG
Sbjct: 297 RATGEEYDELIEEFMSAVKQFYGEKVLIQFEDFANHNAFDLLEKYSKSH--LVFNDDIQG 354
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+ AL++VG L E + GAG AG ++ K N + E
Sbjct: 355 TASVVLAGLLAALKMVGGTLAEQTYLFLGAGEAGTGIAELIALEISKQTNAPIEECRKKV 414
Query: 212 -LVNGKPMVLTKDLDLEKLFPY-RGWLLE----KTNDEGIEGGPEEALKG-ADVLISFTR 264
LV+ K +++ D L P+ + W E KT + ++ L G + V +FT+
Sbjct: 415 WLVDSKGLIV--DSRKGSLQPFKKPWAHEHEPLKTLYDAVQSIKPTVLIGTSGVGRTFTK 472
Query: 265 PGPGVIKPEWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------G 310
+I E + ++ PI+F L+NP E ++A G I A+ G
Sbjct: 473 E---II--EAMSSFNERPIIFSLSNPTSHSECTAEQAYTWSQGRSIFASGSPFAPVEYEG 527
Query: 311 RSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFN 369
++ P Q NN FPG+ G + A + +DM++ A+KA+A ++ E+ I PF
Sbjct: 528 KTFVPGQSNNAYIFPGLGLGLVISGAVRVHEDMLLAASKALADQATQDNFEKGSIFPPFT 587
Query: 370 PI--VYAKEARAVAEEAMKEGVA 390
I + A A AVA +A + G+A
Sbjct: 588 SIRKISAHIAAAVAGKAYELGLA 610
>sp|P36444|MAOC_FLAPR NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PRECURSOR (NADP-ME)
>gi|542102|pir||S42939 malate dehydrogenase
(oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40)
precursor - Flaveria pringlei >gi|1084444|pir||S52016
malate dehydrogenase (oxaloacetate-decarboxylating)
(NADP+) (EC 1.1.1.40) - Flaveria pringlei
>gi|459441|emb|CAA54986.1| (X78069) malate dehydrogenase
(oxaloacetate decarboxylating) (NADP+) [Flaveria
pringlei]
Length = 647
Score = 113 bits (279), Expect = 5e-24
Identities = 129/441 (29%), Positives = 192/441 (43%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ I K+P+ +Y + K V VV+DG R
Sbjct: 189 ELLPIVYTPTVGEACQKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGER 248
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GGI PI I
Sbjct: 249 ILGLGDLG-CQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQR 307
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L+K R VF+DD QG
Sbjct: 308 RASGKEYAELMNEFMSAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTH--LVFNDDIQG 365
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGLI+AL++VG L + GAG AG ++ K N + E
Sbjct: 366 TASVVLAGLISALKLVGGSLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKI 425
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKG--ADVLISFTRPGPG 268
LV+ K +++ LD + F + W +D +A+K VLI + G
Sbjct: 426 WLVDSKGLIVRSRLDSLQHFK-KPW----AHDHEPVNKFLDAVKAIKPTVLIGSSGAGQT 480
Query: 269 VIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVATGRSDYP------- 315
K E + ++ PI+ L+NP E ++A G I A+G P
Sbjct: 481 FTKEVVEAMSSFNEKPIILALSNPTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKV 540
Query: 316 ---NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI 371
Q NN FPG G + A + D+M++ A++A+A V +E + +I PF I
Sbjct: 541 YVSGQSNNAYIFPGFGLGLIISGAIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNI 600
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ A A VA +A + G+A
Sbjct: 601 RKISAHIAAKVAAKAYELGLA 621
>prf||1701292A NADP dependent malic enzyme [Flaveria trinervia]
Length = 648
Score = 112 bits (278), Expect = 7e-24
Identities = 128/442 (28%), Positives = 192/442 (42%), Gaps = 83/442 (18%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L + YTP V E C+ KD ++ E + K V VV+DG R
Sbjct: 189 ELLPIVYTPTVGEACQKYGSIFENSQGLFISLKDKGRILEILKNWPHKKIQVIVVTDGER 248
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 249 ILGLGDLG-CQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNEKLLNDDEFYIGLKQ 307
Query: 103 ----GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQ 155
G++ + E + A+ +G I ED A+ F +L+K R VF+DD Q
Sbjct: 308 KRAAGQEYAELMNEFMSAVKQNYGENLLIQFEDFANHNAFDLLEKYRTTH--LVFNDDIQ 365
Query: 156 GTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE---- 211
GTA+VVL GLI+AL++VG L + GAG AG T ++ K N+ + E
Sbjct: 366 GTASVVLGGLISALKLVGGSLADQKFLFLGAGEAGTGTAELIALEISKQTNIPLEESRKK 425
Query: 212 --LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK--GADVLISFTRPGP 267
LV+ K +++ LD + F + W +D +A+K VLI + G
Sbjct: 426 VWLVDSKGLIVRSRLDSLQHFK-KPW----AHDHEPVNEFLDAIKTIRPTVLIGSSGTGQ 480
Query: 268 GVIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVATGRSDYP------ 315
K E + +++ PI+ L+NP E ++A G I A+G P
Sbjct: 481 TFTKEVVETMSSLNEKPIILALSNPTSQSECTAEQAYTWSEGRAIFASGSPFKPVEYNGK 540
Query: 316 ----NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNP 370
Q NN FPG G + A + DDM++ A++A A V +E ++ +I PF
Sbjct: 541 LYVSGQANNAYIFPGFGLGLIISGAIRVHDDMLLAASEAPAEQVTQEHFDKGLIFPPFTS 600
Query: 371 I--VYAKEARAVAEEAMKEGVA 390
I + A A VA +A + G+A
Sbjct: 601 IRKISAHIAAKVAAKAYELGLA 622
>pir||T48526 NADP dependent malic enzyme-like protein - Arabidopsis thaliana
>gi|7573381|emb|CAB87685.1| (AL163814) NADP dependent
malic enzyme-like protein [Arabidopsis thaliana]
Length = 588
Score = 111 bits (276), Expect = 1e-23
Identities = 124/439 (28%), Positives = 195/439 (44%), Gaps = 78/439 (17%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + +G V VV+DG R
Sbjct: 130 ELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGER 189
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GGI PI I
Sbjct: 190 ILGLGDLG-CQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQR 248
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G + ED A+ F +L K + VF+DD QG
Sbjct: 249 RATGQEYAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLSKYSDSH--LVFNDDIQG 306
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGLI A +V+GK+L + + GAG AG ++ K + E
Sbjct: 307 TASVVLAGLIAAQKVLGKKLADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKI 366
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K ++++ + + F + W E + + G A+K VLI + G
Sbjct: 367 WLVDSKGLIVSSRKESLQHFK-QPWAHEHKPVKDLIGA-VNAIK-PTVLIGTSGVGQTFT 423
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEA---KKAGA---------RIVATGRSDY 314
K E + ++ P++ L+NP E ++A K A +V G++
Sbjct: 424 KEVVEAMATNNEKPLILALSNPTSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYL 483
Query: 315 PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNPI-- 371
P Q NN FPG+ G + A + DDM++ A++A+A+ V EE +I PF+ I
Sbjct: 484 PGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIRE 543
Query: 372 VYAKEARAVAEEAMKEGVA 390
+ A A VA + G+A
Sbjct: 544 ISANIAACVAAKTYDLGLA 562
>pir||C75581 malate oxidoreductase - Deinococcus radiodurans (strain R1)
>gi|6460776|gb|AAF12481.1|AE001863_106 (AE001863) malate
oxidoreductase [Deinococcus radiodurans]
Length = 580
Score = 111 bits (275), Expect = 2e-23
Identities = 120/417 (28%), Positives = 182/417 (42%), Gaps = 64/417 (15%)
Query: 30 YTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGKALLF 89
Y G+ + I + + + + V +D S ILG+G+ G G+ + GK L+
Sbjct: 141 YPRGLTLSTRTIERAEQALANVPLNDVRIIVATDSSAILGIGDQG-FGGMAISIGKLSLY 199
Query: 90 KRFGGID---AFPIMIG--------EQDP-------------------DKFIEVVKAISP 119
GG+ P+ + +DP DKF+E P
Sbjct: 200 TVAGGVGPDKTLPVELDVGTGRADLREDPHYLGVKHERLTGEEYFAFIDKFVEATIQRYP 259
Query: 120 TFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEI 179
I ED + F +L + R+ +P F+DD QGT AVVLAG++NA + G+ L +
Sbjct: 260 K-AIIQWEDFSKDAAFEVLARYRKV--VPSFNDDIQGTGAVVLAGVLNACRIKGEALKDQ 316
Query: 180 SVALFGAGAAG----FATLRILVKAGVKPGNVRVVELVNGKPMVLTKDLDLE---KLFPY 232
V + GAGA G A + + G+ P + V +LT D +E +
Sbjct: 317 VVVVHGAGAGGAGVAAAIREGMHREGLSPDEIARRVFVLDSRGLLTDDRSMEAYKQALAT 376
Query: 233 RGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVIKPEWIREM---SDDPIVFPLAN 289
L+ + + G EA A VL+ + PG + ++ + P+VFPL+N
Sbjct: 377 PRALVSGWSGTDLLGVVREA--KATVLLGLSGV-PGTFDEDIVKAALANTPRPLVFPLSN 433
Query: 290 PV--PEILPDEAKK--AGARIVATGRSDYP----------NQINNLLGFPGIFRGALDVR 335
P E LP++ + GA IVATG P Q NN FPG+ GA+ R
Sbjct: 434 PTAHSEALPEDILRWTDGAAIVATGSPFAPVEHGGQTHEIGQGNNAFIFPGLGFGAILAR 493
Query: 336 ARSITDDMIIEAAKAIASVVEEPSEENIIPSPFNPIVYA--KEARAVAEEAMKEGVA 390
R ITD+M+ EAA A+A + P P N + A + A AV ++A+K+GVA
Sbjct: 494 VREITDEMVTEAAYALADYTQAHHPGRTYP-PVNELSQASVQVAVAVIKQAIKDGVA 549
>pir||JC5967 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) - aloe >gi|2911148|dbj|BAA24950.1| (AB005808)
NADP-malic enzyme [Aloe arborescens]
Length = 592
Score = 110 bits (273), Expect = 3e-23
Identities = 127/445 (28%), Positives = 191/445 (42%), Gaps = 90/445 (20%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ I + P+ +Y + + V VV+DG R
Sbjct: 134 ELLPVVYTPTVGEACQKYGCIFRRPQGLYISLKEKGKILEVLKNWPERNIQVIVVTDGER 193
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GGI P+ I
Sbjct: 194 ILGLGDLG-CQGMGIPVGKLSLYTALGGIRPSACLPVTIDVGTNNEQLLKDEFYIGLRQK 252
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K VF+DD QG
Sbjct: 253 RATGQEYAELLHEFMAAVKQNYGEKVLIQFEDFANHNAFELLAKY--STSHLVFNDDIQG 310
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+ AL++VG L E + GAG AG ++ K V E
Sbjct: 311 TASVVLAGLVAALKLVGGTLAEHTFLFLGAGEAGTGIAELIALEMSKQTKAPVEETRKKI 370
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGA------DVLISFTR 264
LV+ K ++++ D + F + W E P + L GA VLI +
Sbjct: 371 WLVDSKGLIVSSRKDTLQHFK-KPWAHEHE--------PVDTLLGAVKTIKPTVLIGSSG 421
Query: 265 PGPGVIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT--------- 309
G K E + ++ P++ L+NP E +EA G I A+
Sbjct: 422 VGRTFTKEVIEAMSSFTEKPVILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVLY 481
Query: 310 -GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSP 367
G+ P Q NN FPG+ G + A + DDM++ A++A+A V +E +I P
Sbjct: 482 NGKLFVPGQANNAYIFPGLGLGLVISGAIRVHDDMLLAASEALAQQVTQENFANGLIYPP 541
Query: 368 FNPI--VYAKEARAVAEEAMKEGVA 390
F+ I + A+ A VA +A + G+A
Sbjct: 542 FSIIRKISAQIAANVAAKAYELGLA 566
>pir||H75256 malate oxidoreductase - Deinococcus radiodurans (strain R1)
>gi|6460410|gb|AAF12122.1|AE002087_5 (AE002087) malate
oxidoreductase [Deinococcus radiodurans]
Length = 578
Score = 110 bits (273), Expect = 3e-23
Identities = 141/462 (30%), Positives = 204/462 (43%), Gaps = 97/462 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIAK-------------DPEKVYEY----TSKGNSVAVVSDGSR 66
E L + YTP V E K + D E+V E T + V +D S
Sbjct: 110 EMLPIIYTPTVGEAVKLYSNNYRYPRGFTVSTNDIERVEEMLDNVTVNDVRMIVATDSSA 169
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIG---- 103
ILG+G+ G G+ + GK L+ GG+ P+ +G
Sbjct: 170 ILGIGDQG-FGGMAISIGKLSLYTAAGGVGPDKTLPVELDVGTNRQDLIDDPLYLGVHHR 228
Query: 104 -------EQDPDKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
++ D F+E V A P I ED + F +L++ R+ +P F+DD QG
Sbjct: 229 RWQGQQYDEFVDAFVEAVSARYPK-AIIQWEDFSRGTAFRVLERYRKV--VPSFNDDIQG 285
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGF----ATLRILVKAGVKPGNV-RVVE 211
T A+ LAGL+ A + G+ L E + GAGAAG A + L G+ P R V
Sbjct: 286 TGAMALAGLLRASRMKGEFLTEQVFVVVGAGAAGIGVASAIRQGLEAEGLAPEEAGRRVY 345
Query: 212 LVNGKPMVLTKDLDLEKLFPYRGWLLEKTND-EGIEGGPE-----EAL--KGADVLISFT 263
+V+ +++ DLE P + + + D EG G E E L A L+ FT
Sbjct: 346 VVDRYGLLMEGQPDLE---PQQMPFVRRRGDLEGWTFGGEYPTLHEVLVHARATALLGFT 402
Query: 264 RPGPGVIKPEWIREM---SDDPIVFPLANPVP--EILPDE--AKKAGARIVATGRSDYPN 316
PG+ + E I M + PIVFPL+NP E P + A G IVATG S +P+
Sbjct: 403 GV-PGLFRQEDIEAMLAHTPRPIVFPLSNPSSHVEARPSDIVAWTRGGAIVATG-SPFPD 460
Query: 317 -----------QINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEENIIP 365
Q NN FPG+ GA+ RAR ITD+M++ AA +A + + P
Sbjct: 461 VPYDNERIPVGQGNNAFIFPGLGFGAVVSRAREITDNMVMAAALTLAEYTTQHHPHLVYP 520
Query: 366 SPFNPIVYAKE-----ARAVAEEAMKEGV-ARVKVKGEEIEE 401
PI +E A VAE+A+++GV A +V+ + E+
Sbjct: 521 ----PISELRELSIHVAVRVAEQAIQDGVCAERRVRNMDREQ 558
>sp|P22178|MAOC_FLATR NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PRECURSOR (NADP-ME)
>gi|100507|pir||S12893 malate dehydrogenase
(oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40)
precursor - Flaveria trinervia >gi|18460|emb|CAA40421.1|
(X57142) NADP-dependent malic enzyme [Flaveria
trinervia]
Length = 648
Score = 110 bits (272), Expect = 3e-23
Identities = 127/442 (28%), Positives = 191/442 (42%), Gaps = 83/442 (18%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L + YTP V E C+ KD ++ E + K V VV+DG R
Sbjct: 189 ELLPIVYTPTVGEACQKYGSIFENSQGLFISLKDKGRILEILKNWPHKKIQVIVVTDGER 248
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 249 ILGLGDLG-CQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNEKLLNDDEFYIGLKQ 307
Query: 103 ----GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQ 155
G++ + E + A+ +G I ED A+ F +L+K R VF+DD Q
Sbjct: 308 KRAAGQEYAELMNEFMSAVKQNYGENLLIQFEDFANHNAFDLLEKYRTTH--LVFNDDIQ 365
Query: 156 GTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE---- 211
GTA+VVL GLI+AL++VG L + GAG AG ++ K N+ + E
Sbjct: 366 GTASVVLGGLISALKLVGGSLADQKFLFLGAGEAGTGIAELIALEISKQTNIPLEESRKK 425
Query: 212 --LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK--GADVLISFTRPGP 267
LV+ K +++ LD + F + W +D +A+K VLI + G
Sbjct: 426 VWLVDSKGLIVRSRLDSLQHFK-KPW----AHDHEPVNEFLDAIKTIRPTVLIGSSGTGQ 480
Query: 268 GVIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVATGRSDYP------ 315
K E + +++ PI+ L+NP E ++A G I A+G P
Sbjct: 481 TFTKEVVETMSSLNEKPIILALSNPTSQSECTAEQAYTWSEGRAIFASGSPFKPVEYNGK 540
Query: 316 ----NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNP 370
Q NN FPG G + A + DDM++ A++A A V +E ++ +I PF
Sbjct: 541 LYVSGQANNAYIFPGFGLGLIISGAIRVHDDMLLAASEAPAEQVTQEHFDKGLIFPPFTS 600
Query: 371 I--VYAKEARAVAEEAMKEGVA 390
I + A A VA +A + G+A
Sbjct: 601 IRKISAHIAAKVAAKAYELGLA 622
>gb|AAF73006.1|AF262997_1 (AF262997) NADP-dependent malic protein [Ricinus communis]
Length = 641
Score = 110 bits (272), Expect = 3e-23
Identities = 125/446 (28%), Positives = 193/446 (43%), Gaps = 92/446 (20%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I P+ +Y + K V VV+DG R
Sbjct: 183 EMLPIVYTPTVGEACQKYGSIFGRPQGLYISLKEKGRILEVLRNWPEKNIQVIVVTDGER 242
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 243 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQR 301
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G + ED A+ F +L K VF+DD QG
Sbjct: 302 RATGQEYAELLHEFMTAVKQNYGERVLVQFEDFANHNAFDLLAKYGTTH--LVFNDDIQG 359
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+ AL++VG L + GAG AG ++ K N+ V E
Sbjct: 360 TASVVLAGLVAALKLVGGSLADHRFLFLGAGEAGTGIAELIALEMSKQTNMPVEETRKKI 419
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGW---------LLEKTNDEGIEGGPEEALKGADVLIS 261
LV+ K ++++ +D + F R W LL+ ND P + + V +
Sbjct: 420 WLVDSKGLIVSSRMDSLQHFK-RPWAHEHEPIKTLLDAVNDI----KPTVLIGTSGVGRT 474
Query: 262 FTRPGPGVIKPEWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT-------- 309
FT+ E + ++ PI+ L+NP E +EA G I A+
Sbjct: 475 FTKE-----VVEAMASFNEKPIILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVE 529
Query: 310 --GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPS 366
G+ P Q NN FPG G + + DDM++ A++A+A+ V +E ++ +I
Sbjct: 530 YEGKVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVTQENFDKGLIYP 589
Query: 367 PFNPI--VYAKEARAVAEEAMKEGVA 390
PF I + A A VA +A + G+A
Sbjct: 590 PFTNIRKISANIAANVAAKAYELGLA 615
>pir||T06402 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) 2, cytosolic - tomato
>gi|2150029|gb|AAB58728.1| (AF001270) cytosolic
NADP-malic enzyme [Lycopersicon esculentum]
Length = 579
Score = 109 bits (271), Expect = 4e-23
Identities = 122/441 (27%), Positives = 192/441 (42%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ ++ K P+ +Y + K V VV+DG R
Sbjct: 121 ELLPIVYTPTVGEACQKYGSLFKRPQGLYISLNEKGRILEVLKTWPEKSIQVIVVTDGER 180
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 181 ILGLGDLG-CQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLRQR 239
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ D E + A+ +G + ED A+ F +L K R VF+DD QG
Sbjct: 240 RATGQEYYDFLHEFMSAVKQNYGEKILVQFEDFANHNAFELLAKYRTSH--LVFNDDIQG 297
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGLI +L+++G L + + GAG AG ++ N+ V E
Sbjct: 298 TASVVLAGLIASLKLLGGALCDHTFLFLGAGEAGTGIAELIALEISNKTNIPVEETRKKI 357
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K ++++ + + F + W E + +A+K + G G
Sbjct: 358 WLVDSKGLIVSGRKETLQAFK-KPWAHEHEPVNNL----LDAVKAVKPTVLIGTSGTGRT 412
Query: 271 KPEWIRE----MSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
+ + E M+ P++ L+NP E +EA G + A+ G+
Sbjct: 413 FTKEVVEAMACMNKRPLIMALSNPTSQAECTAEEAYTWSEGRAVFASGSPFPSFEYDGKL 472
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNPI 371
+ P Q NN FPG G + + DDM++ A++A+A+ V EE + +I PF I
Sbjct: 473 NIPGQANNCYIFPGFGFGLVMSGTIRVHDDMLLAASEALAAQVTEEHYAKGMIYPPFADI 532
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ A A +VA +A + GVA
Sbjct: 533 RKISAHIAASVAAKAYELGVA 553
>gb|AAF09064.1|AC011717_31 (AC011717) putative malate oxidoreductase; 93001-96525 [Arabidopsis
thaliana]
Length = 646
Score = 109 bits (271), Expect = 4e-23
Identities = 124/452 (27%), Positives = 196/452 (42%), Gaps = 82/452 (18%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L + YTP V E C+ K+ K++E + K V VV+DG R
Sbjct: 188 ELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGER 247
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 248 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQR 306
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
GE+ + E + A+ +G I ED A+ F +L K VF+DD QG
Sbjct: 307 RATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTH--LVFNDDIQG 364
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGLI AL VG L + GAG AG ++ K ++ + E
Sbjct: 365 TASVVLAGLIAALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNI 424
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K ++++ + + F + W +D +A+K + G G
Sbjct: 425 WLVDSKGLIVSSRKESIQHFK-KPW----AHDHEPIRELVDAVKAIKPTVLIGTSGVGQT 479
Query: 271 ----KPEWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
E + ++++ PI+ L+NP E +EA G I A+ G++
Sbjct: 480 FTQDVVETMAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKT 539
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI 371
P Q NN FPG G + + DDM++ A++A+A ++EE E+ +I PF I
Sbjct: 540 FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNI 599
Query: 372 --VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+ A+ A VA +A + G+A + +E+E+
Sbjct: 600 RKISARIAAKVAAKAYELGLATRLPQPKELEQ 631
>emb|CAC18164.1| (AL451013) probable malate dehydrogenase
(oxaloacetate-decarboxylating) (NADP+) [Neurospora
crassa]
Length = 552
Score = 109 bits (271), Expect = 4e-23
Identities = 128/448 (28%), Positives = 197/448 (43%), Gaps = 87/448 (19%)
Query: 23 REHLSLAYTPGVAEPC---KAIAKDPEKVYEYTSKGNSV--------------AVVSDGS 65
+E + L YTP + + C + PE +Y + S+ VV+DGS
Sbjct: 81 KEMMPLVYTPTIGDVCLQYSTLYTRPEALYISIKQRKSIRTILRNWPYPQPEICVVTDGS 140
Query: 66 RILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------GEQDP------- 107
RILGLG++G + G+ + GK L+ GI PI++ +DP
Sbjct: 141 RILGLGDLG-VNGVGIPIGKLALYTAAAGIHPDKTLPIVLDCGTANETNLKDPLYLGLRQ 199
Query: 108 ------------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQ 155
D+F+E + P + ED S K F LD+ R++ F+DD Q
Sbjct: 200 KRIPVAEQQEFMDEFMEAAAEVFPDMV-VQFEDFESEKAFNYLDRYRDQYKC--FNDDIQ 256
Query: 156 GTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE---- 211
GT AVVLAG I A+ + ++E + GAG+AG + LV+ K G
Sbjct: 257 GTGAVVLAGYIGAVNLSNVPIEEQRLVFMGAGSAGVGVAKQLVEYYTKRGLSEQQAKDKF 316
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK--GADVLISFTRPGPG 268
LV+ K +V TKD +KL ++ + N+ EE ++ L+ T G
Sbjct: 317 WLVDTKGLV-TKDRG-DKLAEHKKYFARTDNNGHQFRTLEEVIEYVKPSALVGLTAT-HG 373
Query: 269 VIKPEWIREMSDD---------PIVFPLANPV--PEILPDEA--KKAGARIVATGR---- 311
V +R + PI+FPL+NP+ E EA G + A+G
Sbjct: 374 VFTESVVRALKASVDAGGLGRRPILFPLSNPLTKAECTFKEAIDWTDGTVLFASGSPFSS 433
Query: 312 ------SDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENII 364
+ +PNQ NN+ FPGI GA+ +A +TDDMI +A A+A S+ + ++ +I
Sbjct: 434 YTTNGVTYHPNQGNNVYVFPGIGLGAILAKATRVTDDMIYTSAAALANSLNADEVKQGLI 493
Query: 365 PSPFNPIVYAK--EARAVAEEAMKEGVA 390
+ + A AR V + A +EGV+
Sbjct: 494 YPRIDRVREASVIVAREVMKSARREGVS 521
>pir||A82232 malate oxidoreductase VC1188 [imported] - Vibrio cholerae (group
O1 strain N16961) >gi|9655665|gb|AAF94347.1| (AE004198)
malate oxidoreductase [Vibrio cholerae]
Length = 588
Score = 109 bits (269), Expect = 8e-23
Identities = 122/401 (30%), Positives = 178/401 (43%), Gaps = 81/401 (20%)
Query: 58 VAVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID-------------------AF 98
V VV+DG RILGLG+ G + G+ + GK L+ GGI A
Sbjct: 172 VIVVTDGERILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLAD 230
Query: 99 PIMIGEQDP-------DKFIE-VVKAISPTFGG--INLEDIASPKCFYILDKLREELDIP 148
P+ +G + P D F+E ++A+ + I ED A +L++ + +
Sbjct: 231 PMYMGWRHPRITGPDYDNFVEEFMQAVQRRWPDALIQFEDFAQKNAMPLLERYKNR--VC 288
Query: 149 VFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG--- 205
F+DD QGTAAV + L+ A + G +L + + GAG+AG ++ V G
Sbjct: 289 CFNDDIQGTAAVTVGSLLAACKAAGSQLSQQRITFLGAGSAGCGIAEAIIAQMVSEGISD 348
Query: 206 -----NVRVVE----LVNGKPMVLTKDLDL-EKLFPYRGWLLEKTN---DEGIEGGPEEA 252
V +V+ L G P +L L +K + W E + I
Sbjct: 349 EQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDVIRNAKPTV 408
Query: 253 LKGADVLISFTRPGPGVIKPEWIREMSDD---PIVFPLANPVP--EILPDEAKK--AGAR 305
L G PG+ E I+EM PIVFPL+NP E LP + + G
Sbjct: 409 LVGVS-------GAPGLFSEEIIKEMHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEA 461
Query: 306 IVATGR---------SDYP-NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV 355
+VATG YP Q NN FPGI G L AR +TD+M++E+++A+AS
Sbjct: 462 LVATGSPFDPVLHEGKTYPIVQCNNSYIFPGIGLGVLAANARRVTDEMLMESSRALASC- 520
Query: 356 EEPSEEN----IIPSPFNPI--VYAKEARAVAEEAMKEGVA 390
P N ++P P I V K A AVA++A+++GVA
Sbjct: 521 -SPLAINGHGPLLP-PLESIHSVSKKIAFAVAKKAIEQGVA 559
>pir||T06401 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) precursor - tomato >gi|2150027|gb|AAB58727.1|
(AF001269) NADP-malic enzyme [Lycopersicon esculentum]
Length = 640
Score = 107 bits (264), Expect = 3e-22
Identities = 124/441 (28%), Positives = 189/441 (42%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L + YTP V E C+ K+ K++E + K V VV+DG R
Sbjct: 182 ELLPIVYTPTVGEACQKYGSIFRRPQGLFISLKEKGKIHEVLKNWPEKKIQVIVVTDGER 241
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GGI P+ I
Sbjct: 242 ILGLGDLG-CQGMGIPVGKLSLYTALGGIRPSACLPVTIDVGTNNENLLNDEFYIGLRQR 300
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K VF+DD QG
Sbjct: 301 RATGQEYSELLDEFMYAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTSH--LVFNDDIQG 358
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+ AL +VG L E + GAG AG ++ K + + E
Sbjct: 359 TASVVLAGLMAALNLVGGSLSEHTFLFLGAGEAGTGIAELIALEMSKQTGIPLEETRKKI 418
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPG-V 269
+V+ K +++ +++ + F R W +D A+K + G G
Sbjct: 419 WMVDSKGLIVKSRMEMLQHFK-RPW----AHDHEPVQELVNAVKSIKPTVLIGSSGAGRT 473
Query: 270 IKPEWIREM---SDDPIVFPLANPV--PEILPDEAK--KAGARIVATGRSDYP------- 315
E ++ M ++ PI+F L+NP E +EA G I A+G P
Sbjct: 474 FTKEVVQAMATFNEKPIIFALSNPTSQSECTAEEAYSWSEGRAIFASGSPFAPVEYNGKV 533
Query: 316 ---NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI 371
Q NN FPG G + A + DDM++ A++A+A V +E E+ +I PF+ I
Sbjct: 534 YASGQANNAYIFPGFGLGLIISGAIRVHDDMLLVASEALADEVSQENFEKGLIYPPFSNI 593
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ A VA +A + G+A
Sbjct: 594 RKISAHIRAKVAAKAYELGLA 614
>gb|AAD40139.1|AF149413_20 (AF149413) similar to malate dehydrogenases; Pfam PF00390,
Score=1290.5. E=0, N=1 [Arabidopsis thaliana]
Length = 588
Score = 107 bits (264), Expect = 3e-22
Identities = 126/439 (28%), Positives = 195/439 (43%), Gaps = 78/439 (17%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + +G V VV+DG R
Sbjct: 130 ELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGER 189
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GGI PI I
Sbjct: 190 ILGLGDLG-CQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQK 248
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
GE+ + E + A+ +G + ED A+ F +L K VF+DD QG
Sbjct: 249 RANGEEYAEFLQEFMCAVKQNYGEKVLVQFEDFANHHAFELLSKYCSSH--LVFNDDIQG 306
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAG-----FATLRILVKAGVKPGNVR-VV 210
TA+VVLAGLI A +V+GK L + + GAG AG L+I + G R +
Sbjct: 307 TASVVLAGLIAAQKVLGKSLADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKI 366
Query: 211 ELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K +++++ + + F + W + + + A+K VLI + G
Sbjct: 367 WLVDSKGLIVSERKESLQHFK-QPWAHDHKPVKELLAA-VNAIK-PTVLIGTSGVGKTFT 423
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRSDY 314
K E + +++ P++ L+NP E +EA G I A+ G+
Sbjct: 424 KEVVEAMATLNEKPLILALSNPTSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFT 483
Query: 315 PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNPI-- 371
P Q NN FPG+ G + A + DDM++ A++A+AS V EE +I PF I
Sbjct: 484 PGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRK 543
Query: 372 VYAKEARAVAEEAMKEGVA 390
+ A A +V + + G+A
Sbjct: 544 ISANIAASVGAKTYELGLA 562
>gi|4505143 malic enzyme 1, NADP(+)-dependent, cytosolic; Malic enzyme,
cytoplasmic; malic enzyme 1, soluble [Homo sapiens]
>gi|11418720|ref|XP_004367.1| malic enzyme 1,
NADP(+)-dependent, cytosolic [Homo sapiens]
>gi|1346484|sp|P48163|MAOX_HUMAN NADP-DEPENDENT MALIC
ENZYME (NADP-ME) >gi|1082583|pir||S44415 malate
dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
(EC 1.1.1.40) - human >gi|495123|emb|CAA54460.1|
(X77244) malate dehydrogenase (oxaloacetate
decarboxylating) (NADP+) [Homo sapiens]
>gi|743324|prf||2012237A cytosolic malic enzyme [Homo
sapiens]
Length = 572
Score = 106 bits (263), Expect = 4e-22
Identities = 105/397 (26%), Positives = 175/397 (43%), Gaps = 80/397 (20%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI-------------- 102
VV+DG RILGLG++G G+ + GK L+ GG+ + P+++
Sbjct: 149 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPL 207
Query: 103 ----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
G + D E ++A+S +G I ED A+ F +L+K R +
Sbjct: 208 YIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYC--T 265
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG---- 205
F+DD QGTA+V +AGL+ AL + +L + ++ GAG A ++V A K G
Sbjct: 266 FNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKE 325
Query: 206 -NVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
++ + LV+ K +++ L T ++ E +K + ++ +
Sbjct: 326 KAIKKIWLVDSKGLIVKGRASL-------------TQEKEKFAHEHEEMKNLEAIVQEIK 372
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVAT 309
P G + +++M ++ PI+F L+NP E ++ K G I A+
Sbjct: 373 PTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFAS 432
Query: 310 -----------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
G++ YP Q NN FPG+ G + R ITD++ + A+ IA V +
Sbjct: 433 GSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDK 492
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARV 392
EE + P N I V K A + ++A +E A V
Sbjct: 493 HLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATV 529
>gb|AAC50613.1| (U43944) cytosolic NADP(+)-dependent malic enzyme [Homo sapiens]
Length = 565
Score = 106 bits (263), Expect = 4e-22
Identities = 105/397 (26%), Positives = 175/397 (43%), Gaps = 80/397 (20%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI-------------- 102
VV+DG RILGLG++G G+ + GK L+ GG+ + P+++
Sbjct: 142 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPL 200
Query: 103 ----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
G + D E ++A+S +G I ED A+ F +L+K R +
Sbjct: 201 YIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYC--T 258
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG---- 205
F+DD QGTA+V +AGL+ AL + +L + ++ GAG A ++V A K G
Sbjct: 259 FNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKE 318
Query: 206 -NVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
++ + LV+ K +++ L T ++ E +K + ++ +
Sbjct: 319 KAIKKIWLVDSKGLIVKGRASL-------------TQEKEKFAHEHEEMKNLEAIVQEIK 365
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVAT 309
P G + +++M ++ PI+F L+NP E ++ K G I A+
Sbjct: 366 PTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFAS 425
Query: 310 -----------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
G++ YP Q NN FPG+ G + R ITD++ + A+ IA V +
Sbjct: 426 GSPFDSVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDK 485
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARV 392
EE + P N I V K A + ++A +E A V
Sbjct: 486 HLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATV 522
>gb|AAB08874.1| (U67426) malate dehydrogenase [Vitis vinifera]
Length = 640
Score = 106 bits (263), Expect = 4e-22
Identities = 125/450 (27%), Positives = 196/450 (42%), Gaps = 78/450 (17%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ I + P+ ++ + K V VV+DG R
Sbjct: 182 ELLPVVYTPTVGEACQKYGGILRRPQGLFISLNEKGKILEVLKNWPEKNIQVIVVTDGER 241
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 242 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNQKLLDDEFYIGLRQK 300
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G + ED A+ F +L R VF+DD QG
Sbjct: 301 RATGQEYAELIHEFMCAVKQNYGEKVLVQFEDFANHNAFDLL--ARYGTTHLVFNDDIQG 358
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGLI+AL +VG L E + GAG AG ++ K + E
Sbjct: 359 TASVVLAGLISALNLVGGTLAEHTFLFLGAGEAGTGIAELIALEMSKQTQAPLEETRKKI 418
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K ++++ +D + F + W E + + +A+K VLI + G
Sbjct: 419 WLVDSKGLIVSSRMDSLQHFK-KPWAHEHEPIKNLVDA-VKAIK-PTVLIGSSGVGRTFT 475
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRSDY 314
K E + ++ PI+ L+NP E +EA G I A+ G+
Sbjct: 476 KEVVEAMASFNEKPIILALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPVEYNGKVFV 535
Query: 315 PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI-- 371
P Q NN FPG G + A + DDM++ A++A+A +E ++ +I PF+ I
Sbjct: 536 PGQSNNAYIFPGFGLGLIISGAIRVHDDMLLAASEALAKQATQENFDKGMIYPPFSNIRK 595
Query: 372 VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+ A A VA +A + G+A + E + E
Sbjct: 596 ISAHIAANVAAKAYELGLATRLPQPENLVE 625
>sp|P37223|MAOX_MESCR NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|1084300|pir||S43718
malate dehydrogenase (oxaloacetate-decarboxylating)
(NADP+) (EC 1.1.1.40) - common ice plant
>gi|432380|emb|CAA45772.1| (X64434) malate dehydrogenase
(oxaloacetate decarboxylating) (NADP+) [Mesembryanthemum
crystallinum]
Length = 585
Score = 106 bits (262), Expect = 5e-22
Identities = 127/439 (28%), Positives = 190/439 (42%), Gaps = 78/439 (17%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L L YTP V E C+ KD ++ E + K V VV+DG R
Sbjct: 127 ELLPLVYTPTVGEGCQKYGSIFRRPQGLFISLKDKGRILELLRNWPEKKIQVIVVTDGER 186
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGI---------------------DAFPIMI--- 102
ILGLG++G G+ + GK L+ GG+ D F I +
Sbjct: 187 ILGLGDLG-CQGMGIPVGKLSLYSALGGVCPSACLPITLDVGTNNQKLLDDEFYIGLKQK 245
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
GE+ + E + A+ +G + ED A+ F +L+K R VF+DD QG
Sbjct: 246 RATGEEYAEFVQEFMSAVKQNYGEKILVQFEDFANHNAFELLEKYRTTH--LVFNDDIQG 303
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRV------V 210
TA+VVLAGLI +L+++G L + GAG AG ++ K V +
Sbjct: 304 TASVVLAGLIASLKLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKKTKAPVEQMRKKI 363
Query: 211 ELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K +V++ + + F W E + +A+K VLI + G
Sbjct: 364 WLVDSKGLVVSSRKETLQQFKL-PWAHEHEPITTLIDA-VQAIK-PTVLIGTSGKGKQFT 420
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRSDY 314
K E + ++ P++ L+NP E +EA G I A+ GR+
Sbjct: 421 KEVVEAMANINAKPLILALSNPTSQSECTAEEAYTWSQGHAIFASGSPFDPVEYEGRTFV 480
Query: 315 PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNPI-- 371
P Q NN FPG G + A + DDM++ A++A+AS V E + +I PF I
Sbjct: 481 PGQANNAYIFPGFGLGLIMCGAIRVHDDMLLAASEALASQVTGEHFIKGLIYPPFKDIRK 540
Query: 372 VYAKEARAVAEEAMKEGVA 390
+ A A VA +A + G+A
Sbjct: 541 ISAHIAAGVAAKAYELGLA 559
>gb|AAC62126.1| (AC005169) malate oxidoreductase (malic enzyme) [Arabidopsis
thaliana]
Length = 581
Score = 106 bits (262), Expect = 5e-22
Identities = 124/439 (28%), Positives = 191/439 (43%), Gaps = 78/439 (17%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L + YTP V E C+ KD K+ + + + V VV+DG R
Sbjct: 123 ELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGER 182
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 183 ILGLGDLG-CQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQK 241
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K + VF+DD QG
Sbjct: 242 RATGQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTH--LVFNDDIQG 299
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL++A ++ L E + GAG AG ++ K N V E
Sbjct: 300 TASVVLAGLVSAQKLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKI 359
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K +++ D + F + W E + + G +A+K VLI + G
Sbjct: 360 WLVDSKGLIVNSRKDSLQDFK-KPWAHEHEPVKDLLGA-IKAIK-PTVLIGSSGVGRSFT 416
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVATGRSDYP--------- 315
K E + +++ P++ L+NP E +EA G I A+G P
Sbjct: 417 KEVIEAMSSINERPLIMALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFV 476
Query: 316 -NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI-- 371
Q NN FPG G + A + DDM++ AA+A+A V +E E+ +I F+ I
Sbjct: 477 STQANNAYIFPGFGLGLVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRK 536
Query: 372 VYAKEARAVAEEAMKEGVA 390
+ A+ A VA +A + G+A
Sbjct: 537 ISAQIAANVATKAYELGLA 555
>gb|AAD10504.1| (U39958) NADP-malic enzyme [Zea mays]
Length = 662
Score = 106 bits (262), Expect = 5e-22
Identities = 123/442 (27%), Positives = 194/442 (43%), Gaps = 85/442 (19%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + + V V+DG
Sbjct: 205 ELLPVVYTPTVGEACQKYGSIFRQPQGLYVSLRDKGKVLEVLRNWPHRNIQVICVTDGEA 264
Query: 67 ILGLGNIGPLA-GLPVMEGKALLFKRFGGID---AFPIMI-------------------- 102
ILGLG++G G+PV GK L+ GG+ PI I
Sbjct: 265 ILGLGDLGSQGMGIPV--GKLALYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLRQ 322
Query: 103 ----GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQ 155
GE+ + E + A+ +G I ED A+ F +L K + VF+DD Q
Sbjct: 323 KRATGEEYHELMEEFMAAVKQIYGEKVLIQFEDFANHNAFNLLAKYSKSH--LVFNDDIQ 380
Query: 156 GTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE---- 211
GTA+VVLAGL+ AL+VVG+ L E + GAG AG ++ K + + E
Sbjct: 381 GTASVVLAGLLAALKVVGRTLAEHTYLFLGAGEAGTGIAELIALEISKQTSAPIEECRKK 440
Query: 212 --LVNGKPMVLTKDLDLEKLFPYRGWLLE----KTNDEGIEGGPEEALKG-ADVLISFTR 264
LV+ K +++ + + F + W E T + ++ L G + V +FT+
Sbjct: 441 IWLVDSKGLIVDSRKNSVQSFK-KPWAHEHEPLTTLYDAVQSIKPTVLIGTSGVGRTFTK 499
Query: 265 PGPGVIKPEWIREMSDDPIVFPLANPV--PEILPDEAKKAGARIVATGRSDY-------- 314
E + ++ PI+F L+NP E +EA ++ S +
Sbjct: 500 E-----VVEAMASFNERPIIFSLSNPTSHSECTAEEAYNWTQVVIFASGSPFDPVEYDGK 554
Query: 315 ---PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNP 370
P Q NN FPG G + A + +DM++ A++A+A+ +E ++ I PF
Sbjct: 555 VFVPGQSNNAYIFPGFGLGLVISGAIRVHEDMLLAASEALAAQATQENFDKGSIFPPFTN 614
Query: 371 I--VYAKEARAVAEEAMKEGVA 390
I + A+ ARAVA +A + G+A
Sbjct: 615 IRKISARIARAVAAKAYELGLA 636
>sp|P43279|MAOC_ORYSA NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST PRECURSOR (NADP-ME)
>gi|1076749|pir||S46499 NADP-dependent malic enzyme -
rice >gi|415315|dbj|BAA03949.1| (D16499) NADP-dependent
malic enzyme [Oryza sativa]
Length = 638
Score = 105 bits (259), Expect = 1e-21
Identities = 120/450 (26%), Positives = 193/450 (42%), Gaps = 78/450 (17%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + + V VV+DG R
Sbjct: 180 ELLPVVYTPTVGEACQKYGSIFRQPQGLYVSLKDKGKVLDVLRNWPERNIQVIVVTDGER 239
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 240 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEQLLNDEFYIGLRQR 298
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K + VF+DD QG
Sbjct: 299 RATGKEYHELMEEFMSAVKQIYGEKVLIQFEDFANHNAFDLLAKYSKSH--LVFNDDIQG 356
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+++L+VVG L E + GAG AG ++ K + E
Sbjct: 357 TASVVLAGLLSSLKVVGGTLAEHTYLFLGAGEAGTGIAELIALEISKQTKAPIEECRKKV 416
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
L++ K +++ + + F + W E + + VLI + G
Sbjct: 417 WLLDSKGLIVNSRKESLQAFK-KPWAHEHEPVTTLLDAVQSI--KPTVLIGTSGVGKTFT 473
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRSDY 314
K E + ++ P++F LANP E +EA G + A+ G+
Sbjct: 474 KEVIEAMASFNERPVIFSLANPTSHSECTAEEAYNWSQGRAVFASGSPFDPVEYNGKIHV 533
Query: 315 PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI-- 371
P Q NN FPG G + A + +DM++ A++ +A +E E+ I PF I
Sbjct: 534 PGQSNNAYIFPGFGLGVVISGAVRVHEDMLLAASETLADQATQENFEKGSIFPPFTNIRK 593
Query: 372 VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+ A+ A VA +A + G+A + ++E+
Sbjct: 594 ISARIAATVAAKAYELGLATRLPQPRDLEK 623
>dbj|BAA74735.1| (AB016804) NADP-malic enzyme [Aloe arborescens]
Length = 585
Score = 104 bits (258), Expect = 1e-21
Identities = 123/444 (27%), Positives = 197/444 (43%), Gaps = 88/444 (19%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ I + P+ +Y + + V VV+DG R
Sbjct: 127 ELLPVVYTPTVGEACQKYGCIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGER 186
Query: 67 ILGLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMI-------------------- 102
ILGLG++G G+PV GK L+ GGI P+ I
Sbjct: 187 ILGLGDLGSQGMGIPV--GKLSLYTALGGIHPSSCLPVTIDVGTNNEQLLNDEFYIGLRQ 244
Query: 103 ----GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQ 155
G++ + E + A+ +G + ED A+ F +L K + VF+DD Q
Sbjct: 245 KRATGQEYAELLHEFMTAVKQNYGEKVIVQYEDFANHNAFELLAKYSKSH--LVFNDDIQ 302
Query: 156 GTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRIL-------VKAGVKPGNVR 208
GTA+VVLAGL+ AL+VVG+ L E + GAG AG ++ KA ++ +
Sbjct: 303 GTASVVLAGLVAALKVVGRTLAEHTFLFLGAGEAGTGIAELIALEMSRQTKAPIEETR-K 361
Query: 209 VVELVNGKPMVLTKDLDLEKLFPYRGWLLE----KTNDEGIEG-GPEEALKGADVLISFT 263
+ LV+ K ++++ + + F + W E KT + ++ P + + V +FT
Sbjct: 362 KIWLVDSKGLIVSSRKESLQHFK-KPWAHEHEPVKTLLDAVKTIKPTVLIGSSGVGQTFT 420
Query: 264 RPGPGVIKPEWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT---------- 309
+ E + ++ P++ L+NP E ++A G I A+
Sbjct: 421 KE-----VAEAMPSFNEKPVILALSNPTSQSECTAEQAYSWSEGRAIFASGSPFDPVEYN 475
Query: 310 GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPF 368
G+ P Q NN FPG+ G + A + D+M++ A++A+A V EE +I PF
Sbjct: 476 GKLFVPGQANNAYIFPGLGLGLVISGAIRVHDEMLLAASEALAQQVTEENFANGLIYPPF 535
Query: 369 NPI--VYAKEARAVAEEAMKEGVA 390
I + A A VA +A + G+A
Sbjct: 536 TIIRKISAHIAANVAAKAYELGLA 559
>sp|P37222|MAOC_LYCES NADP-DEPENDENT MALIC ENZYME, CHLOROPLAST (NADP-ME)
>gi|7431229|pir||T07088 malate dehydrogenase
(oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40) -
tomato (fragment) >gi|440845|gb|AAA34174.1| (L27509)
malate dehydrogenase [Lycopersicon esculentum]
Length = 573
Score = 104 bits (257), Expect = 2e-21
Identities = 124/442 (28%), Positives = 185/442 (41%), Gaps = 86/442 (19%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E L + YTP V E C+ K+ K++E + K V VV+DG R
Sbjct: 117 ELLPIVYTPTVGEACQKYGWIFKRPQGLFFSLKEKGKIHEVLKNWPEKKIQVIVVTDGER 176
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMIGEQDPDKFI------------ 111
ILGLG++G G+ + GK L+ GGI P+ I KF+
Sbjct: 177 ILGLGDLG-CQGMGIPVGKLSLYSALGGIRPSACLPVTIDVGQTMKFVDDEFYIGLRQRR 235
Query: 112 -----------EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
E + A+ +G I ED A+ F +L K VF+DD QGT
Sbjct: 236 ATGQEYSELLDEFMYAVKQNYGEKVLIQFEDFANHNAFNLLAKYGTSH--LVFNDDIQGT 293
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE------ 211
A+VVLAGL+ AL +VG L E + GAG AG ++ K + + E
Sbjct: 294 ASVVLAGLMAALNLVGGSLSEHTFLFLGAGEAGTGIAELIALEMSKQTGIPLEETRKKIW 353
Query: 212 LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPG-VI 270
+V+ K +++ +++ + F R W +D A+K + G G
Sbjct: 354 MVDSKGLIVKSRMEMLQHFK-RPW----AHDHEPVQELVNAVKSIKPTVLIGSSGAGRTF 408
Query: 271 KPEWIREM---SDDPIVFPLANPV--PEILPDEAK--KAGARIVATGRSDYP-------- 315
E ++ M ++ PI+F L+NP E +EA G I A+G P
Sbjct: 409 TKEVVQAMATFNEKPIIFALSNPTSQSECTAEEAYSWSEGRAIFASGSPFAPVEYNGKVY 468
Query: 316 --NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEENI-----IPSPF 368
Q NN FPG G + A + DDM++ A++A+A +E S+EN IP
Sbjct: 469 ASGQANNAYIFPGFGLGLIISGAIRVHDDMLLVASEALA---DEVSQENFEKGTHIPPFS 525
Query: 369 NPIVYAKEARAVAEEAMKEGVA 390
N + VA +A + G+A
Sbjct: 526 NIRKISAHIAKVAAKAYELGLA 547
>emb|CAB64263.1| (AJ251546) malate dehydrogenase (NADP-dependent oxaloacetate
decarboxylating), malic enzyme [Drosophila melanogaster]
Length = 604
Score = 104 bits (257), Expect = 2e-21
Identities = 118/405 (29%), Positives = 173/405 (42%), Gaps = 78/405 (19%)
Query: 26 LSLAYTPGVAEPCKA---IAKDPEKVY-EYTSKGNSVAV-------------VSDGSRIL 68
+ + YTP V C+ I + P ++ Y +G+ V V+DG RIL
Sbjct: 136 MPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERIL 195
Query: 69 GLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMIGE--------QDP--------- 107
GLG++G G+PV GK L+ GI PI++ +DP
Sbjct: 196 GLGDLGACGMGIPV--GKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKR 253
Query: 108 ------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
D+FI E ++A+ +G I ED + F LDK R F+DD QGT
Sbjct: 254 VVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYC--TFNDDIQGT 311
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKP 217
A+V +AGL + + GK + + GAG A + VKA V+ G V + E N
Sbjct: 312 ASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDG-VPIEEAYNRIY 370
Query: 218 MV-----LTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVIKP 272
MV LTK + L ++ + N +K VLI G+ P
Sbjct: 371 MVDIDGLLTKSRKVGNLDGHKIHYAKDINPMSDLAEIVSTIK-PSVLIG-ASAAAGIFTP 428
Query: 273 EWIREMSDD---PIVFPLANPVPE---ILPDEAKKAGARIVATGRSD-----------YP 315
E +R M+D+ P+VF L+NP + D K AR++ + S YP
Sbjct: 429 EILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYP 488
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE 360
Q NN FPG+ G + I D+M + AA+ +A+ V EPS+
Sbjct: 489 GQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFV-EPSD 532
>gb|AAF56674.1| (AE003759) Mdh gene product [Drosophila melanogaster]
Length = 617
Score = 104 bits (257), Expect = 2e-21
Identities = 118/405 (29%), Positives = 173/405 (42%), Gaps = 78/405 (19%)
Query: 26 LSLAYTPGVAEPCKA---IAKDPEKVY-EYTSKGNSVAV-------------VSDGSRIL 68
+ + YTP V C+ I + P ++ Y +G+ V V+DG RIL
Sbjct: 136 MPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERIL 195
Query: 69 GLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMIGE--------QDP--------- 107
GLG++G G+PV GK L+ GI PI++ +DP
Sbjct: 196 GLGDLGACGMGIPV--GKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKR 253
Query: 108 ------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
D+FI E ++A+ +G I ED + F LDK R F+DD QGT
Sbjct: 254 VVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYC--TFNDDIQGT 311
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKP 217
A+V +AGL + + GK + + GAG A + VKA V+ G V + E N
Sbjct: 312 ASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDG-VPIEEAYNRIY 370
Query: 218 MV-----LTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVIKP 272
MV LTK + L ++ + N +K VLI G+ P
Sbjct: 371 MVDIDGLLTKSRKVGNLDGHKIHYAKDINPMSDLAEIVSTIK-PSVLIG-ASAAAGIFTP 428
Query: 273 EWIREMSDD---PIVFPLANPVPE---ILPDEAKKAGARIVATGRSD-----------YP 315
E +R M+D+ P+VF L+NP + D K AR++ + S YP
Sbjct: 429 EILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYP 488
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE 360
Q NN FPG+ G + I D+M + AA+ +A+ V EPS+
Sbjct: 489 GQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFV-EPSD 532
>dbj|BAB03427.1| (AP002816) ESTs AU091509(C10754),D24805(R2584),C25822(C10754),
D39717(S1291) correspond to a region of the predicted
gene.~Similar to Oryza sativa NADP-dependent malic
enzyme, chloroplast precursor (P43279)
>gi|9711835|dbj|BAB07934.1| (AP002836) Oryza sativa
NADP-dependent malic enzyme, chloroplast precursor
Length = 639
Score = 104 bits (257), Expect = 2e-21
Identities = 119/450 (26%), Positives = 194/450 (42%), Gaps = 78/450 (17%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + + V VV+DG R
Sbjct: 181 ELLPVVYTPTVGEACQKYGSIFRQPQGLYVSLKDKGKVLDVLRNWPERNIQVIVVTDGER 240
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 241 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEQLLNDEFYIGLRQR 299
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K + VF+DD QG
Sbjct: 300 RATGKEYHELMEEFMSAVKQIYGEKVLIQFEDFANHNAFDLLAKYSKSH--LVFNDDIQG 357
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAGL+++L+VVG L E + GAG AG ++ K + E
Sbjct: 358 TASVVLAGLLSSLKVVGGTLAEHTYLFLGAGEAGTGIAELIALEISKQTKAPIEECRKKV 417
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
L++ K +++ + + F + W E + + VLI + G
Sbjct: 418 WLLDSKGLIVNSRKESLQAFK-KPWAHEHEPVTTLLDAVQSI--KPTVLIGTSGVGKTFT 474
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRSDY 314
K E + ++ P++F L+NP E +EA G + A+ G+
Sbjct: 475 KEVIEAMASFNERPVIFSLSNPTSHSECTAEEAYNWSQGRAVFASGSPFDPVEYNGKIHV 534
Query: 315 PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI-- 371
P Q NN FPG G + A + +DM++ A++ +A +E E+ I PF I
Sbjct: 535 PGQSNNAYIFPGFGLGVVISGAVRVHEDMLLAASETLADQATQENFEKGSIFPPFTNIRK 594
Query: 372 VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+ A+ A +VA +A + G+A + ++E+
Sbjct: 595 ISARIAASVAAKAYELGLATRLPQPRDLEK 624
>sp|P13697|MAOX_RAT NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|65937|pir||DERTMX malate
dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
(EC 1.1.1.40) - rat
Length = 572
Score = 104 bits (257), Expect = 2e-21
Identities = 111/406 (27%), Positives = 179/406 (43%), Gaps = 80/406 (19%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMIGE--------QDP- 107
VV+DG RILGLG++G G+ + GK L+ GG++ PI + +DP
Sbjct: 149 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPL 207
Query: 108 --------------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
D F+ E ++A S +G I ED A+ F +L+K R +
Sbjct: 208 YIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANLNAFRLLNKYRNKYC--T 265
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVR- 208
F+DD QGTA+V +AGL+ AL + +L + +V GAG A ++V A K G +
Sbjct: 266 FNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEKEGLSKE 325
Query: 209 ----VVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
+ LV+ K +++ L T ++ + E +K + ++ +
Sbjct: 326 KARQKIWLVDSKGLIVKGRASL-------------TEEKEVFAHEHEEMKNLEAIVQKIK 372
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKKA--GARIVAT 309
P G + +++M ++ PI+F L+NP E +E K G I A+
Sbjct: 373 PTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEECYKVTKGRAIFAS 432
Query: 310 -----------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
GR+ +P Q NN FPG+ G + R I D + + A+ I+ V +
Sbjct: 433 GSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHINDSVFLTTAEVISQQVSDK 492
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
EE + P N I V K A + ++A KE +A V + + EE
Sbjct: 493 HLEEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEE 538
>gi|7106353 malic enzyme 1, soluble [Rattus norvegicus]
>gi|205294|gb|AAA41563.1| (M26594) malic enzyme [Rattus
norvegicus]
Length = 586
Score = 104 bits (257), Expect = 2e-21
Identities = 111/406 (27%), Positives = 179/406 (43%), Gaps = 80/406 (19%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMIGE--------QDP- 107
VV+DG RILGLG++G G+ + GK L+ GG++ PI + +DP
Sbjct: 149 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPL 207
Query: 108 --------------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
D F+ E ++A S +G I ED A+ F +L+K R +
Sbjct: 208 YIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANLNAFRLLNKYRNKYC--T 265
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVR- 208
F+DD QGTA+V +AGL+ AL + +L + +V GAG A ++V A K G +
Sbjct: 266 FNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEKEGLSKE 325
Query: 209 ----VVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
+ LV+ K +++ L T ++ + E +K + ++ +
Sbjct: 326 KARQKIWLVDSKGLIVKGRASL-------------TEEKEVFAHEHEEMKNLEAIVQKIK 372
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKKA--GARIVAT 309
P G + +++M ++ PI+F L+NP E +E K G I A+
Sbjct: 373 PTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEECYKVTKGRAIFAS 432
Query: 310 -----------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
GR+ +P Q NN FPG+ G + R I D + + A+ I+ V +
Sbjct: 433 GSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHINDSVFLTTAEVISQQVSDK 492
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
EE + P N I V K A + ++A KE +A V + + EE
Sbjct: 493 HLEEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEE 538
>sp|P28227|MAOX_ANAPL NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|104473|pir||S23435 malate
dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
(EC 1.1.1.40) - duck >gi|62460|emb|CAA47049.1| (X66418)
malate dehydrogenase (oxaloacetate decarboxylating)
(NADP+) [Aix sp.]
Length = 557
Score = 103 bits (255), Expect = 3e-21
Identities = 115/406 (28%), Positives = 179/406 (43%), Gaps = 82/406 (20%)
Query: 60 VVSDGSRILGLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMI------------- 102
VV+DG RILGLG++G G+PV GK L+ GG+ + P+M+
Sbjct: 138 VVTDGERILGLGDLGCYGMGIPV--GKLALYTACGGVKPHECLPVMLDVGTDNEALLKDP 195
Query: 103 -----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIP 148
G+ D E ++A++ +G I ED A+ F +L K R +
Sbjct: 196 LYIGLRHKRIRGQAYDDLLDEFMEAVTSRYGMNCLIQFEDFANANAFRLLHKYRNKYC-- 253
Query: 149 VFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG--- 205
F+DD QGTA+V +AGL+ AL + RL + +V GAG A ++V A K G
Sbjct: 254 TFNDDIQGTASVAVAGLLAALRITKNRLSDHTVLFQGAGEAALGIANLIVMAMEKEGVSK 313
Query: 206 --NVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKT---NDEGIEGGPEEALKG--ADV 258
V+ + +V+ K +++ L EKT ++ E+ +K V
Sbjct: 314 EAAVKRIWMVDSKGLIVKGRASL---------TAEKTRFAHEHAEMKNLEDIVKDIKPSV 364
Query: 259 LISFTRPGPGVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVAT-- 309
LI G G E +++M + PI+F L+NP E ++ K G I A+
Sbjct: 365 LIGVAAIG-GAFTKEILQDMAAFNKRPIIFALSNPTSKAECTAEQCYKYTEGRGIFASGS 423
Query: 310 ---------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSE 360
G++ YP Q NN FPG+ G + + I +D+ + A+ IA E+ SE
Sbjct: 424 PFDPVTLPNGKTLYPGQGNNSYVFPGVALGVIACGLKHIGEDVFLTTAEVIA---EQVSE 480
Query: 361 ENIIPSPFNP------IVYAKEARAVAEEAMKEGVARVKVKGEEIE 400
EN+ P V K A +AEEA + A + +++E
Sbjct: 481 ENLQEGRLYPPLVTIQHVSLKIAVRIAEEAYRNNTASTYPQPKDLE 526
>pir||JC4160 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) - human
Length = 572
Score = 103 bits (255), Expect = 3e-21
Identities = 105/406 (25%), Positives = 178/406 (42%), Gaps = 80/406 (19%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI-------------- 102
VV+DG RILGLG++G G+ + GK L+ GG+ + P+++
Sbjct: 149 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPL 207
Query: 103 ----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
G + D E ++A+S +G I ED A+ F +L+K R +
Sbjct: 208 YIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYC--T 265
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG---- 205
F+DD QGTA+V +AGL+ AL + +L + ++ GAG A ++V A K G
Sbjct: 266 FNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKE 325
Query: 206 -NVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
++ + LV+ K +++ L T ++ E +K + ++ +
Sbjct: 326 KAIKKIWLVDSKGLIVKGRASL-------------TQEKEKFAHEHEEMKNLEAIVQEIK 372
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVAT 309
P G + +++M ++ PI+F L+NP E ++ K G I A+
Sbjct: 373 PTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFAS 432
Query: 310 -----------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
GR+ +P Q NN FPG+ G + R I D + + A+ I+ V +
Sbjct: 433 GSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTAEVISQQVSDK 492
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+E + P N I V K A + ++A KE +A V + + EE
Sbjct: 493 HLQEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEE 538
>gi|7019429 NADP-dependent malic enzyme [Homo sapiens]
>gi|1335389|gb|AAB01380.1| (L34035) NADP-dependent malic
enzyme [Homo sapiens]
Length = 572
Score = 103 bits (255), Expect = 3e-21
Identities = 105/406 (25%), Positives = 178/406 (42%), Gaps = 80/406 (19%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI-------------- 102
VV+DG RILGLG++G G+ + GK L+ GG+ + P+++
Sbjct: 149 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPL 207
Query: 103 ----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
G + D E ++A+S +G I ED A+ F +L+K R +
Sbjct: 208 YIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYC--T 265
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG---- 205
F+DD QGTA+V +AGL+ AL + +L + ++ GAG A ++V A K G
Sbjct: 266 FNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKE 325
Query: 206 -NVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
++ + LV+ K +++ L T ++ E +K + ++ +
Sbjct: 326 KAIKKIWLVDSKGLIVKGRASL-------------TQEKEKFAHEHEEMKNLEAIVQEIK 372
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVAT 309
P G + +++M ++ PI+F L+NP E ++ K G I A+
Sbjct: 373 PTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFAS 432
Query: 310 -----------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
GR+ +P Q NN FPG+ G + R I D + + A+ I+ V +
Sbjct: 433 GSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTAEVISQQVSDK 492
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+E + P N I V K A + ++A KE +A V + + EE
Sbjct: 493 HLQEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEE 538
>sp|P34105|MAOX_POPTR NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|20469|emb|CAA39690.1|
(X56233) malic enzyme [Populus balsamifera subsp.
trichocarpa]
Length = 591
Score = 102 bits (253), Expect = 6e-21
Identities = 121/441 (27%), Positives = 190/441 (42%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I K P+ +Y + K V VV+DG R
Sbjct: 133 ELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQKSIQVIVVTDGER 192
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 193 ILGLGDLG-CQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLLKDEFYIGLRQR 251
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K VF+DD QG
Sbjct: 252 RATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH--LVFNDDIQG 309
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TAAVVLAGLI+AL+++G L + + GAG AG ++ + + E
Sbjct: 310 TAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKTPLEETRKKI 369
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPG-V 269
L + K ++++ + + F + W E +G+ E +K ++ G G
Sbjct: 370 WLTDSKGLIVSSRKESLQHFK-KPWAHEHEPVKGL----LEVVKAIKPIVLIGTSGVGKT 424
Query: 270 IKPEWIREM---SDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
E I M ++ P++ L+NP E EA G I A+ G+
Sbjct: 425 FTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVEYEGKV 484
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI 371
P Q NN FPG+ G + A + DDM++ AA+A+A + EE + +I P + I
Sbjct: 485 FVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYPPLSNI 544
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ + A VA +A + G+A
Sbjct: 545 RKISVQIAANVAAKAYELGLA 565
>sp|P27443|MAOM_ASCSU NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL PRECURSOR (NAD-ME)
Length = 643
Score = 102 bits (252), Expect = 8e-21
Identities = 116/414 (28%), Positives = 181/414 (43%), Gaps = 91/414 (21%)
Query: 23 REHLSLAYTPGVAEPCKA---IAKDPEKVYEYTSKGNSVA-----------------VVS 62
+E + + YTP V C+ I + P+ +Y T NSV+ VV+
Sbjct: 157 KELMPIVYTPTVGLACQNFGYIYRKPKGLY-ITINDNSVSKIYQILSNWHEEDVRAIVVT 215
Query: 63 DGSRILGLGNIGPLA-GLPVMEGKALLFKRFGGID---AFPIMIGE-------------- 104
DG RILGLG++G G+PV GK L+ GG+ P+++
Sbjct: 216 DGERILGLGDLGAYGIGIPV--GKLALYVALGGVQPKWCLPVLLDVGTNNMDLLNDPFYI 273
Query: 105 ---------QDPDKFIE-VVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFH 151
+D D ++ +KA + +G I ED A+P F +LDK +++ + F+
Sbjct: 274 GLRHKRVRGKDYDTLLDNFMKACTKKYGQKTLIQFEDFANPNAFRLLDKYQDKYTM--FN 331
Query: 152 DDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE 211
DD QGTA+V++AGL+ V K + + FGAGAA ++V G + E
Sbjct: 332 DDIQGTASVIVAGLLTCTRVTKKLVSQEKYLFFGAGAASTGIAEMIVHQMQNEG-ISKEE 390
Query: 212 LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGP---- 267
N + +D++ L + + + + PE +I RPG
Sbjct: 391 ACNRIYL-----MDIDGLVTKNRKEMNPRHVQFAKDMPETT--SILEVIRAARPGALIGA 443
Query: 268 ----GVIKPEWIR---EMSDDPIVFPLANPVP--EILPDEAKKA--GARIVATGRSDYPN 316
G E IR E+++ PI+F L+NP E +EA GA + A+G S +PN
Sbjct: 444 STVRGAFNEEVIRAMAEINERPIIFALSNPTSKAECTAEEAYTFTNGAALYASG-SPFPN 502
Query: 317 -----------QINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPS 359
Q NN FPG+ G + + R + +D+ + AAK +AS V E S
Sbjct: 503 FELNGHTYKPGQGNNAYIFPGVALGTILFQIRHVDNDLFLLAAKKVASCVTEDS 556
>pir||S29742 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) - pig roundworm
Length = 617
Score = 102 bits (252), Expect = 8e-21
Identities = 116/414 (28%), Positives = 181/414 (43%), Gaps = 91/414 (21%)
Query: 23 REHLSLAYTPGVAEPCKA---IAKDPEKVYEYTSKGNSVA-----------------VVS 62
+E + + YTP V C+ I + P+ +Y T NSV+ VV+
Sbjct: 131 KELMPIVYTPTVGLACQNFGYIYRKPKGLY-ITINDNSVSKIYQILSNWHEEDVRAIVVT 189
Query: 63 DGSRILGLGNIGPLA-GLPVMEGKALLFKRFGGID---AFPIMIGE-------------- 104
DG RILGLG++G G+PV GK L+ GG+ P+++
Sbjct: 190 DGERILGLGDLGAYGIGIPV--GKLALYVALGGVQPKWCLPVLLDVGTNNMDLLNDPFYI 247
Query: 105 ---------QDPDKFIE-VVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFH 151
+D D ++ +KA + +G I ED A+P F +LDK +++ + F+
Sbjct: 248 GLRHKRVRGKDYDTLLDNFMKACTKKYGQKTLIQFEDFANPNAFRLLDKYQDKYTM--FN 305
Query: 152 DDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE 211
DD QGTA+V++AGL+ V K + + FGAGAA ++V G + E
Sbjct: 306 DDIQGTASVIVAGLLTCTRVTKKLVSQEKYLFFGAGAASTGIAEMIVHQMQNEG-ISKEE 364
Query: 212 LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGP---- 267
N + +D++ L + + + + PE +I RPG
Sbjct: 365 ACNRIYL-----MDIDGLVTKNRKEMNPRHVQFAKDMPETT--SILEVIRAARPGALIGA 417
Query: 268 ----GVIKPEWIR---EMSDDPIVFPLANPVP--EILPDEAKKA--GARIVATGRSDYPN 316
G E IR E+++ PI+F L+NP E +EA GA + A+G S +PN
Sbjct: 418 STVRGAFNEEVIRAMAEINERPIIFALSNPTSKAECTAEEAYTFTNGAALYASG-SPFPN 476
Query: 317 -----------QINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPS 359
Q NN FPG+ G + + R + +D+ + AAK +AS V E S
Sbjct: 477 FELNGHTYKPGQGNNAYIFPGVALGTILFQIRHVDNDLFLLAAKKVASCVTEDS 530
>sp|P40927|MAOX_COLLI NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|630994|pir||S43231 malate
dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
(EC 1.1.1.40), cytosolic - pigeon
>gi|466430|gb|AAA49450.1| (L09233) malate dehydrogenase
(NADP+) [Columba livia]
Length = 557
Score = 102 bits (252), Expect = 8e-21
Identities = 112/404 (27%), Positives = 178/404 (43%), Gaps = 78/404 (19%)
Query: 60 VVSDGSRILGLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMI------------- 102
VV+DG RILGLG++G G+PV GK L+ GG+ P+M+
Sbjct: 138 VVTDGERILGLGDLGCYGMGIPV--GKLALYTACGGVKPHQCLPVMLDVGTDNETLLKDP 195
Query: 103 -----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIP 148
G+ D E ++A++ +G I ED A+ F +L K R +
Sbjct: 196 LYIGLRHKRIRGQAYDDLLDEFMEAVTSRYGMNCLIQFEDFANANAFRLLHKYRNKYC-- 253
Query: 149 VFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG--- 205
F+DD QGTA+V +AGL+ AL + RL + +V GAG A ++V A K G
Sbjct: 254 TFNDDIQGTASVAVAGLLAALRITKNRLSDHTVLFQGAGEAALGIANLIVMAMQKEGVSK 313
Query: 206 --NVRVVELVNGKPMVLTKDLDL---EKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLI 260
++ + +V+ K +++ L ++ F + ++ D + P VLI
Sbjct: 314 EEAIKRIWMVDSKGLIVKGRASLTPEKEHFAHEHCEMKNLEDIVKDIKP-------TVLI 366
Query: 261 SFTRPGPGVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVA----- 308
G G + +++M + PI+F L+NP E ++ K G I A
Sbjct: 367 GVAAIG-GAFTQQILQDMAAFNKRPIIFALSNPTSKAECTAEQLYKYTEGRGIFASGSPF 425
Query: 309 ------TGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEEN 362
+G++ YP Q NN FPG+ G + + I DD+ + A+ IA +E SEEN
Sbjct: 426 DPVTLPSGQTLYPGQGNNSYVFPGVALGVISCGLKHIGDDVFLTTAEVIA---QEVSEEN 482
Query: 363 IIPSPFNP------IVYAKEARAVAEEAMKEGVARVKVKGEEIE 400
+ P V K A +A+EA + A + E++E
Sbjct: 483 LQEGRLYPPLVTIQQVSLKIAVRIAKEAYRNNTASTYPQPEDLE 526
>prf||1803524A malic enzyme [Populus trichocarpa x Populus deltoides]
Length = 589
Score = 102 bits (251), Expect = 1e-20
Identities = 122/441 (27%), Positives = 191/441 (42%), Gaps = 84/441 (19%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I K P+ +Y + K V VV+DG R
Sbjct: 133 ELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQKSIQVIVVTDGER 192
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 193 ILGLGDLG-CQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLLKDEFYIGLRQR 251
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K VF+DD QG
Sbjct: 252 RATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH--LVFNDDIQG 309
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TAAVVLAGLI+AL+++G L + + GAG AG ++ + + E
Sbjct: 310 TAAVVLAGLISALKLIGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKTPLEETRKKI 369
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPG-V 269
L + K ++++ + + F + W E +G+ E +K L+ G G
Sbjct: 370 WLTDSKGLIVSSRKESLQHFK-KPWAHEHEPVKGL----LEVVKAIKPLVLIGTSGVGKT 424
Query: 270 IKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVAT----------GRS 312
E I M ++ P++ L+NP E +EA G I A+ G+
Sbjct: 425 FTKEVIEAMASFNEKPLILALSNPTSQSECTAEEAYTWGKGKAIFASGSPFDPVEYEGKV 484
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI 371
P Q NN FPG+ G + A + DDM++ AA+A+A + EE + +I P + I
Sbjct: 485 FVPGQSNNAYIFPGL--GLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYPPLSNI 542
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ + A VA +A + G+A
Sbjct: 543 RKISVQIAANVAAKAYELGLA 563
>gi|6678912 malic enzyme, supernatant [Mus musculus]
>gi|126738|sp|P06801|MAOX_MOUSE NADP-DEPENDENT MALIC
ENZYME (NADP-ME) >gi|65938|pir||DEMSMX malate
dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
(EC 1.1.1.40) - mouse >gi|199021|gb|AAA39489.1| (M26756)
malic enzyme [Mus musculus] >gi|199763|gb|AAA39727.1|
(J02652) malate oxidoreductase [Mus musculus]
Length = 572
Score = 101 bits (249), Expect = 2e-20
Identities = 110/406 (27%), Positives = 179/406 (43%), Gaps = 80/406 (19%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMIGE--------QDP- 107
VV+DG RILGLG++G G+ + GK L+ GG++ PI + +DP
Sbjct: 149 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPL 207
Query: 108 --------------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
D F+ E ++A S +G I ED A+ F +L+K R +
Sbjct: 208 YIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYC--T 265
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPG 205
F+DD QGTA+V +AGL+ AL + +L + +V GAG A ++V K G+
Sbjct: 266 FNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLVVMAMEKEGLSKE 325
Query: 206 NVR-VVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
N R + LV+ K +++ L T ++ + E +K + ++ +
Sbjct: 326 NARKKIWLVDSKGLIVKGRASL-------------TEEKEVFAHEHEEMKNLEAIVQKIK 372
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKKA--GARIVAT 309
P G + +++M ++ PI+F L++P E DE K G I A+
Sbjct: 373 PTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSSPTSKAECSADECYKVTKGRAIFAS 432
Query: 310 -----------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
GR+ +P Q NN FPG+ G + R I D + + + I+ V +
Sbjct: 433 GSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHIDDKVFLTTREVISQQVSDK 492
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+E + P N I V K A + ++A KE +A V + + EE
Sbjct: 493 HLQEGRLYPPLNTIRGVSLKIAVKIVQDAYKEKMATVYPEPQNKEE 538
>sp|P26616|MAO1_ECOLI NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|7431233|pir||B64901 malate
dehydrogenase (oxaloacetate-decarboxylating) (EC
1.1.1.38), NAD-linked - Escherichia coli
>gi|1742411|dbj|BAA15127.1| (D90788) SfcA protein
(fragment). [Escherichia coli]
>gi|1742421|dbj|BAA15136.1| (D90789) SfcA protein
(fragment). [Escherichia coli]
>gi|1742432|dbj|BAA15146.1| (D90790) SfcA protein
(fragment). [Escherichia coli]
>gi|1787754|gb|AAC74552.1| (AE000245) NAD-linked malate
dehydrogenase (malic enzyme) [Escherichia coli K12]
Length = 574
Score = 101 bits (249), Expect = 2e-20
Identities = 125/456 (27%), Positives = 192/456 (41%), Gaps = 85/456 (18%)
Query: 24 EHLSLAYTPGVAEPCKAIAKDPEK----VYEYTSKGN-------------SVAVVSDGSR 66
E + + YTP V C+ ++ + Y ++ N V VV+DG R
Sbjct: 107 EMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGER 166
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGEQDP 107
ILGLG+ G + G+ + GK L+ GGI P+ +G ++P
Sbjct: 167 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNP 225
Query: 108 -----------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
D+FI+ VK P + ED A +L++ R E I F+DD QG
Sbjct: 226 RITDDEYYEFVDEFIQAVKQRWPDVL-LQFEDFAQKNAMPLLNRYRNE--ICSFNDDIQG 282
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPGNVRVVEL 212
TAAV + LI A G +L E + GAG+AG +++ + G+ R
Sbjct: 283 TAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVF 342
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADV-------LISFTRP 265
+ + +LT + L P++ L++K + + L DV ++
Sbjct: 343 MVDRFGLLTD--KMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSG 400
Query: 266 GPGVIKPEWIREMSDD---PIVFPLANPVP--EILPDE--AKKAGARIVATGRSDYP--- 315
G+ E IREM PIV PL+NP E P + A G +VATG P
Sbjct: 401 QTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVW 460
Query: 316 -------NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA--SVVEEPSEENIIPS 366
Q NN FPGI G + A ITD+M++ A++ +A S + E ++P
Sbjct: 461 KDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMVLPE 520
Query: 367 PFN-PIVYAKEARAVAEEAMKEGVARVKVKGEEIEE 401
+ V A AV + A ++GVA VK E +++
Sbjct: 521 LKDIQKVSRAIAFAVGKMAQQQGVA-VKTSAEALQQ 555
>emb|CAA50716.1| (X71897) malolactic enzyme [Lactococcus lactis]
Length = 521
Score = 100 bits (247), Expect = 3e-20
Identities = 111/425 (26%), Positives = 184/425 (43%), Gaps = 70/425 (16%)
Query: 25 HLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEG 84
+L + + + E K A D E + VV+D ILG+G+ G G+ + G
Sbjct: 94 YLDINHPENITETLKNAAGDREI---------RLIVVTDAEGILGIGDWGT-QGVDISVG 143
Query: 85 KALLFKRFGGID---AFPIMI------------------------GEQD---PDKFIEVV 114
K +++ GID P++I G+Q D+F+E
Sbjct: 144 KLMIYTAAAGIDPASVLPVVIDAGTNRKELLEDHLYLGNHQERIYGDQYYSFVDQFVETA 203
Query: 115 KAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGK 174
++I P ++ ED IL+ + + IP F+DD QGT VVL G+ +L++ G+
Sbjct: 204 ESIFPKLY-LHWEDFGRSNAATILNNYKTK--IPTFNDDIQGTGIVVLGGIFGSLDITGE 260
Query: 175 RLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDL---DLEKLFP 231
+L + +G G+AG A + V A + + E M+ + L D+E L P
Sbjct: 261 KLTDQVYLCYGGGSAG-AGIAGRVHAEMVSEGLSEEEAYKHFFMIDQQGLLFDDMEDLTP 319
Query: 232 YRGWLLEKTNDEGIEGGPEEALKGA----DVLISFTRPGPGVIKPEWIREM---SDDPIV 284
+ +K D G + L ++ T PG E + M ++ P++
Sbjct: 320 AQKPFAKKRADYKDAGDMTDLLNVVKTVKPTILVGTSTNPGAFTKEVVEAMCANTERPVI 379
Query: 285 FPLANPVPEILPDEAK----KAGARIVATG---------RSDYP-NQINNLLGFPGIFRG 330
FP++NP ++ + G VATG DY Q NN L +PG+ G
Sbjct: 380 FPISNPTKKMETTAEQVIEWSDGKAFVATGVPSGTISYKGVDYQIGQANNSLIYPGLGLG 439
Query: 331 ALDVRARSITDDMIIEAAKAIASVVE--EPSEENIIPSPFNPIVYAKEARAVAEEAMKEG 388
L A+ +TD+MI AA +++ +V+ +P + P F V K A AVA++A ++G
Sbjct: 440 MLASEAKLLTDEMIGAAAHSLSGLVDPGKPGAPVLPPFEFVADVSIKVAEAVAKKAQEQG 499
Query: 389 VARVK 393
+ K
Sbjct: 500 LTESK 504
>pir||S43057 malolactic enzyme - Lactococcus lactis >gi|2130997|emb|CAA53589.1|
(X75982) malolactic enzyme [Lactococcus lactis]
Length = 540
Score = 100 bits (247), Expect = 3e-20
Identities = 111/425 (26%), Positives = 184/425 (43%), Gaps = 70/425 (16%)
Query: 25 HLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEG 84
+L + + + E K A D E + VV+D ILG+G+ G G+ + G
Sbjct: 113 YLDINHPENITETLKNAAGDREI---------RLIVVTDAEGILGIGDWGT-QGVDISVG 162
Query: 85 KALLFKRFGGID---AFPIMI------------------------GEQD---PDKFIEVV 114
K +++ GID P++I G+Q D+F+E
Sbjct: 163 KLMIYTAAAGIDPASVLPVVIDAGTNRKELLEDHLYLGNHQERIYGDQYYSFVDQFVETA 222
Query: 115 KAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGK 174
++I P ++ ED IL+ + + IP F+DD QGT VVL G+ +L++ G+
Sbjct: 223 ESIFPKLY-LHWEDFGRSNAATILNNYKTK--IPTFNDDIQGTGIVVLGGIFGSLDITGE 279
Query: 175 RLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDL---DLEKLFP 231
+L + +G G+AG A + V A + + E M+ + L D+E L P
Sbjct: 280 KLTDQVYLCYGGGSAG-AGIAGRVHAEMVSEGLSEEEAYKHFFMIDQQGLLFDDMEDLTP 338
Query: 232 YRGWLLEKTNDEGIEGGPEEALKGA----DVLISFTRPGPGVIKPEWIREM---SDDPIV 284
+ +K D G + L ++ T PG E + M ++ P++
Sbjct: 339 AQKPFAKKRADYKDAGDMTDLLNVVKTVKPTILVGTSTNPGAFTKEVVEAMCANTERPVI 398
Query: 285 FPLANPVPEILPDEAK----KAGARIVATG---------RSDYP-NQINNLLGFPGIFRG 330
FP++NP ++ + G VATG DY Q NN L +PG+ G
Sbjct: 399 FPISNPTKKMETTAEQVIEWSDGKAFVATGVPSGTISYKGVDYQIGQANNSLIYPGLGLG 458
Query: 331 ALDVRARSITDDMIIEAAKAIASVVE--EPSEENIIPSPFNPIVYAKEARAVAEEAMKEG 388
L A+ +TD+MI AA +++ +V+ +P + P F V K A AVA++A ++G
Sbjct: 459 MLASEAKLLTDEMIGAAAHSLSGLVDPGKPGAPVLPPFEFVADVSIKVAEAVAKKAQEQG 518
Query: 389 VARVK 393
+ K
Sbjct: 519 LTESK 523
>emb|CAB66003.1| (AJ132257) NADP-dependent malate dehydrogenase (decarboxylating)
[Apium graveolens]
Length = 570
Score = 100 bits (247), Expect = 3e-20
Identities = 117/448 (26%), Positives = 196/448 (43%), Gaps = 78/448 (17%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + K V VV+DG R
Sbjct: 112 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKNIQVIVVTDGER 171
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 172 ILGLGDLG-CQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNESLLNNEFYIGLKQR 230
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ D E + A+ +G + ED A+ F +L K VF+DD QG
Sbjct: 231 RATGKEYADLLQEFMTAVKQNYGEKVLVQFEDFANHNAFELLSKY--STTHLVFNDDIQG 288
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVL+G++++L+++G L + + GAG AG ++ K + + E
Sbjct: 289 TASVVLSGIVSSLKLIGGMLADHTFLFLGAGEAGTGIAELIALEMSKQTKIHLEETRKKI 348
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI 270
LV+ K ++++ + + F + W E + +A+K VLI + G
Sbjct: 349 WLVDSKGLIVSHRKESLQHFK-KPWAHEHEPCTTLIDA-VKAIK-PTVLIGTSGQGKTFT 405
Query: 271 KP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVATG----------RSDY 314
K E + + P++ L+NP E +EA G + A+G +
Sbjct: 406 KEVVEAMASFNKKPLIMALSNPTSQAECTAEEAYNWSEGRAVFASGSPFDPVKYNNQLYI 465
Query: 315 PNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI-- 371
P Q NN FPG+ G + A + D+M++ A++A+A V +E ++ + PF+ I
Sbjct: 466 PGQANNAYIFPGLGLGLVMSGAIRMHDEMLLAASEALACQVTQEHYDKGMTFPPFSNIRT 525
Query: 372 VYAKEARAVAEEAMKEGVARVKVKGEEI 399
+ A A VA +A G+A + E++
Sbjct: 526 ISANIAAKVAAKAYDLGLATRLPRPEDL 553
>sp|P37225|MAON_SOLTU NAD-DEPENDENT MALIC ENZYME 59 KDA ISOFORM, MITOCHONDRIAL PRECURSOR
(NAD-ME) >gi|1076666|pir||A53318 malate dehydrogenase
(decarboxylating) (EC 1.1.1.39) 59K chain precursor,
mitochondrial - potato >gi|438131|emb|CAA80547.1|
(Z23002) precursor of the 59kDa subunit of the
mitochondrial NAD+-dependent malic enzyme [Solanum
tuberosum]
Length = 601
Score = 100 bits (246), Expect = 4e-20
Identities = 126/447 (28%), Positives = 203/447 (45%), Gaps = 93/447 (20%)
Query: 48 VYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMIGE 104
++ + S + V++DGSRILGLG++G + G+ + GK ++ GI+ P+M+
Sbjct: 164 IFNWPSTQVDMIVLTDGSRILGLGDLG-VQGIGIPIGKLDMYVAAAGINPQRVLPVMLDV 222
Query: 105 --------QDP-------------------DKFIEVVKAISPTFGGINLEDIASPKCFYI 137
+DP D+F+E V A P + ED + F
Sbjct: 223 GTNNQKLLEDPLYLGLRQPRLEGEEYLSIVDEFVEAVHARWPK-AVVQFEDFQAKWAFET 281
Query: 138 LDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS---VALFGAGAAGFATL 194
LD+ R++ + F+DD QGTA V LAGL+ + G+ L + + + + GAG+AG L
Sbjct: 282 LDRYRKKFCM--FNDDIQGTAGVALAGLLGTVRAQGRPLTDFANQKIVVVGAGSAGLGVL 339
Query: 195 RILVKAGVK---PGNVRVVELVNGKPMVLT--KDLDLEKLFPYRGWLLEKTNDEGIEG-- 247
++ ++A + P L++ ++ KD+D L P+ K + E IEG
Sbjct: 340 KMALQAVSRMTGPSADPHFFLLDKNGLITKDRKDIDPAAL-PFA-----KAHHE-IEGLG 392
Query: 248 -----GPEEALKGAD--VLISFTRPGPGVIKPEWIREMSDD----PIVFPLANPV--PEI 294
G E +K VL+ + G G+ E +R M + P +F ++NP E
Sbjct: 393 LQEGAGLAEVVKKVKPHVLLGLSGVG-GIFHEEVLRAMKESDSVRPAIFAMSNPTNNAEC 451
Query: 295 LPDEA-KKAGARIV------------ATGRSDYPNQINNLLGFPGIFRGALDVRARSITD 341
P +A K AG IV G+ + NQ NN+ FPGI GAL AR+I+D
Sbjct: 452 CPVDAFKLAGEDIVFASGSPFANVDLGNGKIGHVNQANNMYLFPGIGLGALLSGARNISD 511
Query: 342 DMIIEAAKAIASVVEE---------PSEENIIPSPFNPIVYAKEARAVAEEAMKEGVARV 392
M+ AA+ +AS + + PS ++I V A RA E + EG V
Sbjct: 512 TMLEAAAECLASYMSDDEINRGILYPSIDDI--RDITAEVGAAVLRAAVAEDLAEGHGDV 569
Query: 393 KVKGEE--IEERTLKLI--SLWDDILG 415
VK + +E T++ + ++W + G
Sbjct: 570 GVKELQHMSKEETIEHVRQNMWYPVYG 596
>sp|Q48662|MLES_LACLA MALOLACTIC ENZYME >gi|541361|pir||S38728 malolactic enzyme (EC
1.1.1.-) - Lactococcus lactis >gi|737262|prf||1922245A
malolactic enzyme [Lactococcus lactis]
Length = 540
Score = 100 bits (246), Expect = 4e-20
Identities = 111/425 (26%), Positives = 184/425 (43%), Gaps = 70/425 (16%)
Query: 25 HLSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEG 84
+L + + + E K+ A D E + VV+D ILG+G+ G G+ + G
Sbjct: 113 YLDINHPENITETLKSAAGDREI---------RLIVVTDAEGILGIGDWGT-QGVDISVG 162
Query: 85 KALLFKRFGGID---AFPIMI------------------------GEQD---PDKFIEVV 114
K +++ GID P++I G+Q D+F+E
Sbjct: 163 KLMIYTAAAGIDPASVLPVVIDAGTNRKELLEDHLYLGNHQERIYGDQYYSFVDQFVETA 222
Query: 115 KAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGK 174
++I P ++ ED IL+ + + IP F+DD QGT VVL G+ +L++ G+
Sbjct: 223 ESIFPKLY-LHWEDFGRSNAATILNNYKTK--IPTFNDDIQGTGIVVLGGIFGSLDITGE 279
Query: 175 RLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVNGKPMVLTKDL---DLEKLFP 231
+L + +G G+AG A + V A + + E M+ + L D+E L P
Sbjct: 280 KLTDQVYLCYGGGSAG-AGIAGRVHAEMVSEGLSEEEAYKHFFMIDQQGLLFDDMEDLTP 338
Query: 232 YRGWLLEKTNDEGIEGGPEEALKGA----DVLISFTRPGPGVIKPEWIREM---SDDPIV 284
+ +K D G + L ++ T PG E + M ++ P++
Sbjct: 339 AQKPFAKKRADYKDAGDMTDLLNVVKTVKPTILVGTSTNPGAFTKEVVEAMCANTERPVI 398
Query: 285 FPLANPVPEILPDEAK----KAGARIVATG---------RSDYP-NQINNLLGFPGIFRG 330
FP++NP ++ + G VATG DY Q NN L PG+ G
Sbjct: 399 FPISNPTKKMETTAEQVIEWSDGKAFVATGVPSGTISYKGVDYQIGQANNSLIHPGLGLG 458
Query: 331 ALDVRARSITDDMIIEAAKAIASVVE--EPSEENIIPSPFNPIVYAKEARAVAEEAMKEG 388
L A+ +TD+MI AA +++ +V+ +P + P F V K A AVA++A ++G
Sbjct: 459 MLASEAKLLTDEMIGAAAHSLSGLVDPGKPGAPVLPPFEFVADVSIKVAEAVAKKAQEQG 518
Query: 389 VARVK 393
+ K
Sbjct: 519 LTESK 523
>sp|P45868|MAO2_BACSU PROBABLE NAD-DEPENDENT MALIC ENZYME (NAD-ME)
>gi|1075863|pir||S55433 malate dehydrogenase homolog
ywkA - Bacillus subtilis >gi|853772|emb|CAA89880.1|
(Z49782) malolactic enzyme [Bacillus subtilis]
>gi|2636230|emb|CAB15722.1| (Z99122) similar to malate
dehydrogenase [Bacillus subtilis]
>gi|2636242|emb|CAB15733.1| (Z99123) similar to malate
dehydrogenase [Bacillus subtilis]
Length = 582
Score = 100 bits (246), Expect = 4e-20
Identities = 121/449 (26%), Positives = 194/449 (42%), Gaps = 88/449 (19%)
Query: 23 REHLSLAYTPGVAEPCKAIAKD---PEKVY----------------EYTSKGNSVAVVSD 63
RE L + YTP V E + + + P+ +Y T+ + V +D
Sbjct: 115 REMLPVVYTPTVGEAIQEYSHEYRRPQGIYLSIDNIDGIEKAFENLHATAGDIDLIVATD 174
Query: 64 GSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGE 104
ILG+G+ G + G+ + GK ++ GID P+ IG
Sbjct: 175 SESILGIGDWG-VGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLLNDPLYIGN 233
Query: 105 QDP-------DKFIEV-VKAISPTFGG--INLEDIASPKCFYILDKLREELDIPVFHDDQ 154
+ + FI+ VKA F ++ ED+ + I+ K E I F+DD
Sbjct: 234 KHERVQGERYEAFIDAYVKAALKFFPKALLHWEDLGNKNARNIMKKYNHE--ILTFNDDI 291
Query: 155 QGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATL----RILVKAGVKPGNVRVV 210
QGT A+ LAG++ A++ G + + V +FGAG+AG +V AG+
Sbjct: 292 QGTGAITLAGVLAAMKKTGASIKDQRVVIFGAGSAGIGIADQIRDTMVLAGLSEEEANKR 351
Query: 211 ELVNGKPMVLTKDLD--LEKLFPY-------RGWLLEKTNDEGIEGGPEEALKGADVLIS 261
+LT+D++ L+ PY + W K +++G E + ++
Sbjct: 352 FYTLDYRGLLTEDIEGILDFQKPYLRNADEVKDW---KRDEKGQIPFDEVVRQAKPTILI 408
Query: 262 FTRPGPGVIKPEWIREMS---DDPIVFPLANP--VPEILPDEAKK--AGARIVATGRSDY 314
T G E ++EM+ D P++ P++NP + E +P++ K G ++ATG S +
Sbjct: 409 GTSGVSGAFTEEIVKEMASHVDRPVIMPMSNPTHLAEAVPEDLFKWTDGKVLIATG-SPF 467
Query: 315 PN-----------QINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVE-EPSEEN 362
N Q NN FPG+ G++ AR IT M A AIA +V+ E
Sbjct: 468 DNVEYNGVSYEIGQSNNAFAFPGLGLGSIVAEARIITPAMFAATADAIAEMVDLETPGAG 527
Query: 363 IIPSPFN-PIVYAKEARAVAEEAMKEGVA 390
++PS V + A AVAE A+K+GVA
Sbjct: 528 LLPSIDKLQEVSIQVAIAVAEAAIKDGVA 556
>pir||S18826 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) (clone 064) - western balsam poplar x
cottonwood
Length = 589
Score = 99.8 bits (245), Expect = 5e-20
Identities = 121/441 (27%), Positives = 190/441 (42%), Gaps = 84/441 (19%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I K P+ +Y + K V VV+DG R
Sbjct: 133 ELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQKSIQVIVVTDGER 192
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ P+ I
Sbjct: 193 ILGLGDLG-CQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLLKDEFYIGLRQR 251
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + A+ +G I ED A+ F +L K VF+DD QG
Sbjct: 252 RATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH--LVFNDDIQG 309
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TAAVVLAGLI+AL+++G L + + GAG AG ++ + + E
Sbjct: 310 TAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKTPLEETRKKI 369
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPG-V 269
L + K ++++ + + F + W E +G+ E +K ++ G G
Sbjct: 370 WLTDSKGLIVSSRKESLQHFK-KPWAHEHEPVKGL----LEVVKAIKPIVLIGTSGVGKT 424
Query: 270 IKPEWIREM---SDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
E I M ++ P++ L+NP E EA G I A+ G+
Sbjct: 425 FTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVEYEGKV 484
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI 371
P Q NN FPG+ G + A + DDM++ AA+A+A + EE + +I P + I
Sbjct: 485 FVPGQSNNAYIFPGL--GLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYPPLSNI 542
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ + A VA +A + G+A
Sbjct: 543 RKISVQIAANVAAKAYELGLA 563
>sp|Q29558|MAOX_PIG NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|1066278|emb|CAA63599.1|
(X93016) malate dehydrogenase decarboxylase (NADP+) [Sus
scrofa]
Length = 557
Score = 99.5 bits (244), Expect = 7e-20
Identities = 100/397 (25%), Positives = 170/397 (42%), Gaps = 80/397 (20%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGI---DAFPIMI-------------- 102
VV+DG RILGLG++G G+ + GK L+ GG+ + P+++
Sbjct: 135 VVTDGERILGLGDLG-CNGMGIPVGKLALYTACGGVNPQECLPVILDVGTENEELLKDPL 193
Query: 103 ----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPV 149
G + D E ++A+S +G I ED A+ F +L K + +
Sbjct: 194 YIGLRQRRVRGPEYDDFLDEFMEAVSSKYGMNCLIQFEDFANINAFRLLKKYQNQYC--T 251
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG---- 205
F+DD QGTA+V +AG++ AL + +L + ++ GAG A ++V A K G
Sbjct: 252 FNDDIQGTASVAVAGILAALRITKNKLSDQTILFQGAGEAALGIAHLIVMAMEKEGVPKE 311
Query: 206 -NVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTR 264
++ + LV+ K +++ L TN++ E +K + ++ +
Sbjct: 312 KAIKKIWLVDSKGLIVKGRAAL-------------TNEKEEFAHEHEEMKNLEAIVQDIK 358
Query: 265 PGP--------GVIKPEWIREM---SDDPIVFPLANPVPEILPDEAK----KAGARIVA- 308
P G + +++M ++ PI+F L+NP + + G I A
Sbjct: 359 PTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECTAERGYTLTQGRAIFAS 418
Query: 309 ----------TGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP 358
+G++ YP Q NN FPG+ + R ITD + + A+ IA V +
Sbjct: 419 GSPFDPVTLPSGQTLYPGQGNNSYVFPGVALAVVACGLRHITDKIFLTTAEVIAQQVSDK 478
Query: 359 S-EENIIPSPFNPI--VYAKEARAVAEEAMKEGVARV 392
EE + P N I V K A + +A +E A +
Sbjct: 479 HLEEGRLYPPLNTIRDVSLKIAEKIVRDAYQEKTATI 515
>sp|P51615|MAOX_VITVI NADP-DEPENDENT MALIC ENZYME (NADP-ME) >gi|515759|gb|AAA67087.1|
(L34836) malate dehydrogenase (NADP+) [Vitis vinifera]
Length = 591
Score = 98.3 bits (241), Expect = 1e-19
Identities = 118/441 (26%), Positives = 191/441 (42%), Gaps = 82/441 (18%)
Query: 24 EHLSLAYTPGVAEPCK---AIAKDPEKVY--------------EYTSKGNSVAVVSDGSR 66
E L + YTP V E C+ +I + P+ +Y + + V VV+DG R
Sbjct: 133 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERRIQVIVVTDGER 192
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI--------------------- 102
ILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 193 ILGLGDLG-CQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKLLANEFYIGLKQR 251
Query: 103 ---GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
G++ + E + + +G I ED A+ F +L K F+DD QG
Sbjct: 252 RATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTH--LAFNDDIQG 309
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE----- 211
TA+VVLAG+++AL ++G L + GAG AG ++ K + E
Sbjct: 310 TASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTKCPIEETRKKI 369
Query: 212 -LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK--GADVLISFTRPGPG 268
LV+ K +++ D + F + W E + + +A+K VLI + G
Sbjct: 370 WLVDSKGLIVGSRKDSLQQFK-KPWAHEHEPVKDL----LDAVKVIKPTVLIGSSGVGKA 424
Query: 269 VIKP--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRS 312
K E + ++ P++ L+NP E +EA G I A+ G++
Sbjct: 425 FTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVEYNGKT 484
Query: 313 DYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI 371
P Q NN FPG+ G + A + D+M++ A++A+A V +E ++ +I PF+ I
Sbjct: 485 FVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYPPFSNI 544
Query: 372 --VYAKEARAVAEEAMKEGVA 390
+ A A VA +A + G+A
Sbjct: 545 RKISAHIAANVAAKAYELGLA 565
>gb|AAC13636.2| (AF058919) F6N23.16 gene product [Arabidopsis thaliana]
Length = 606
Score = 97.9 bits (240), Expect = 2e-19
Identities = 107/408 (26%), Positives = 180/408 (43%), Gaps = 75/408 (18%)
Query: 48 VYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI-- 102
+Y + + + V++DGSRILGLG++G + G+ + GK ++ GI+ PIM+
Sbjct: 170 IYNWPAPQVDMIVITDGSRILGLGDLG-VQGIGIPIGKLDMYVAAAGINPQRVLPIMLDV 228
Query: 103 --------------GEQDP-----------DKFIEVVKAISPTFGGINLEDIASPKCFYI 137
G + P D+F+E P + ED + F
Sbjct: 229 GTNNEKLLQNDLYLGVRQPRLEGEEYLEIIDEFMEAAFTRWPK-AVVQFEDFQAKWAFGT 287
Query: 138 LDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEI---SVALFGAGAAGFATL 194
L++ R++ + F+DD QGTA V LAGL+ + G+ + + + + GAG+AG
Sbjct: 288 LERYRKKFCM--FNDDVQGTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLGVT 345
Query: 195 RILVKAGVKPGNVRVVE------LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGG 248
++ V+A + + E L++ +V T+ L+ G +L N I G
Sbjct: 346 KMAVQAVARMAGISESEATKNFYLIDKDGLVTTERTKLDP-----GAVLFAKNPAEIREG 400
Query: 249 PE--EALKGA--DVLISFTRPGPGVIKPEWIREMSDD----PIVFPLANPVPEI---LPD 297
E +K VL+ + G G+ E ++ M + P +F ++NP D
Sbjct: 401 ASIVEVVKKVRPHVLLGLSGVG-GIFNEEVLKAMRESDSCKPAIFAMSNPTLNAECTAAD 459
Query: 298 EAKKAGARIV------------ATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMII 345
K AG IV G+ + NQ NN+ FPGI G L AR +TD M+
Sbjct: 460 AFKHAGGNIVFASGSPFENVELENGKVGHVNQANNMYLFPGIGLGTLLSGARIVTDGMLQ 519
Query: 346 EAAKAIAS-VVEEPSEENIIPSPFNPI--VYAKEARAVAEEAMKEGVA 390
A++ +AS + +E ++ I+ N I + A+ AV A+ + +A
Sbjct: 520 AASECLASYMTDEEVQKGILYPSINNIRHITAEVGAAVLRAAVTDDIA 567
>pir||T01221 malate dehydrogenase (decarboxylating) (EC 1.1.1.39) precursor,
mitochondrial - Arabidopsis thaliana
>gi|7267396|emb|CAB80866.1| (AL161472) putative malate
oxidoreductase [Arabidopsis thaliana]
Length = 606
Score = 97.9 bits (240), Expect = 2e-19
Identities = 107/408 (26%), Positives = 180/408 (43%), Gaps = 75/408 (18%)
Query: 48 VYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI-- 102
+Y + + + V++DGSRILGLG++G + G+ + GK ++ GI+ PIM+
Sbjct: 170 IYNWPAPQVDMIVITDGSRILGLGDLG-VQGIGIPIGKLDMYVAAAGINPQRVLPIMLDV 228
Query: 103 --------------GEQDP-----------DKFIEVVKAISPTFGGINLEDIASPKCFYI 137
G + P D+F+E P + ED + F
Sbjct: 229 GTNNEKLLQNDLYLGVRQPRLEGEEYLEIIDEFMEAAFTRWPK-AVVQFEDFQAKWAFGT 287
Query: 138 LDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEI---SVALFGAGAAGFATL 194
L++ R++ + F+DD QGTA V LAGL+ + G+ + + + + GAG+AG
Sbjct: 288 LERYRKKFCM--FNDDVQGTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLGVT 345
Query: 195 RILVKAGVKPGNVRVVE------LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGG 248
++ V+A + + E L++ +V T+ L+ G +L N I G
Sbjct: 346 KMAVQAVARMAGISESEATKNFYLIDKDGLVTTERTKLDP-----GAVLFAKNPAEIREG 400
Query: 249 PE--EALKGA--DVLISFTRPGPGVIKPEWIREMSDD----PIVFPLANPVPEI---LPD 297
E +K VL+ + G G+ E ++ M + P +F ++NP D
Sbjct: 401 ASIVEVVKKVRPHVLLGLSGVG-GIFNEEVLKAMRESDSCKPAIFAMSNPTLNAECTAAD 459
Query: 298 EAKKAGARIV------------ATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMII 345
K AG IV G+ + NQ NN+ FPGI G L AR +TD M+
Sbjct: 460 AFKHAGGNIVFASGSPFENVELENGKVGHVNQANNMYLFPGIGLGTLLSGARIVTDGMLQ 519
Query: 346 EAAKAIAS-VVEEPSEENIIPSPFNPI--VYAKEARAVAEEAMKEGVA 390
A++ +AS + +E ++ I+ N I + A+ AV A+ + +A
Sbjct: 520 AASECLASYMTDEEVQKGILYPSINNIRHITAEVGAAVLRAAVTDDIA 567
>gb|AAG13628.1|AC078840_19 (AC078840) putative mitochondrial NAD+-dependent malic enzyme
protein [Oryza sativa]
Length = 520
Score = 97.1 bits (238), Expect = 3e-19
Identities = 108/365 (29%), Positives = 162/365 (43%), Gaps = 68/365 (18%)
Query: 48 VYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI-- 102
+Y + ++ + VV+DGSRILGLG++G + G+ + GK ++ GI+ PIM+
Sbjct: 160 IYNWPAEKVDMIVVTDGSRILGLGDLG-VQGIGIPIGKLDVYVAAAGINPQKVLPIMLDV 218
Query: 103 --------------GEQDP-----------DKFIEVVKAISPTFGGINLEDIASPKCFYI 137
G + P D+F+E V A P + ED F
Sbjct: 219 GTNNTKLLEDKLYLGLRQPRLEGEEYLSVVDEFMEAVHARWPK-AVVQFEDFQMKWAFET 277
Query: 138 LDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEIS---VALFGAGAAGFATL 194
L + R + F+DD QGTA V LAGL+ A+ G+ L + + + + GAG+AG L
Sbjct: 278 LQRYRNRFCM--FNDDVQGTAGVALAGLLGAVRAQGRPLSDFTKQKIVVVGAGSAGIGVL 335
Query: 195 RILVKAGVK-PGNVRVVELVN-----GKPMVLTKD---LDLEKLFPYRGWLLEKTNDEGI 245
+ +A ++ PG R E N K ++TK LD RG+ E+ D
Sbjct: 336 NMAKQAMLRMPGINRSGEGHNQFWVLDKDGLITKSRKGLDPAVARYARGFGPEEVQDLHE 395
Query: 246 EGGPEEALKGAD--VLISFTRPGPGVIKPEWIREMSDD----PIVFPLANPVP--EILPD 297
E ++ VL+ + G G+ E ++ M + P +F ++NP E P+
Sbjct: 396 GASLVEVVRKVKPHVLLGLSGVG-GIFNEEVLKAMKESDSPRPAIFAMSNPTTKAECTPE 454
Query: 298 EA-KKAGARIV------------ATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMI 344
+ K G V GR Y NQ NN+ FPGI GAL AR ITD M+
Sbjct: 455 DVFKYVGDNAVFASGSPFSNVTLGNGRQGYANQANNMYLFPGIGLGALLSGARHITDGML 514
Query: 345 IEAAK 349
AA+
Sbjct: 515 QSAAE 519
>sp|P71880|MAOX_MYCTU PUTATIVE MALATE OXIDOREDUCTASE [NAD] (MALIC ENZYME)
>gi|7431223|pir||E70705 probable malate oxidoreductase -
Mycobacterium tuberculosis (strain H37RV)
>gi|1524268|emb|CAB02059.1| (Z79702) mez [Mycobacterium
tuberculosis]
Length = 652
Score = 94.8 bits (232), Expect = 2e-18
Identities = 117/426 (27%), Positives = 178/426 (41%), Gaps = 78/426 (18%)
Query: 26 LSLAYTPGVAEPCKAIAKDPEKVYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGK 85
LS+ + E + PE V + V +D ILG+G+ G + G+ + GK
Sbjct: 223 LSIDEPDEIEEAFNTLGLGPEDV--------DLIVCTDAEAILGIGDWG-VGGIQIAVGK 273
Query: 86 ALLFKRFGGID-------------------AFPIMIGEQDP-----------DKFIEVVK 115
L+ GG+D A P +G + ++IE +
Sbjct: 274 LALYTAGGGVDPRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQ 333
Query: 116 AISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKR 175
+ P ++ ED ILD D VF+DD QGT AVVLA + + L+V G
Sbjct: 334 RLFPR-AILHFEDFGPANARKILDTYGT--DYCVFNDDMQGTGAVVLAAVYSGLKVTGIP 390
Query: 176 LDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVELVN-----GKPMVLTKDLDLEKLF 230
L + ++ +FGAG AG + A V G + + V+ +P +L D+D + F
Sbjct: 391 LRDQTIVVFGAGTAGMGIADQIRDAMVADG-ATLEQAVSQIWPIDRPGLLFDDMDDLRDF 449
Query: 231 --PYRGWLLEKTNDEGIEGGPEEALKGADVLISFT-----RPGPGVIKPEWIREMS---D 280
PY + + G+ G L A + S T G E + M+
Sbjct: 450 QVPY----AKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCK 505
Query: 281 DPIVFPLANPVP--EILPDE--AKKAGARIVATGRSDYP----------NQINNLLGFPG 326
P++FPL+NP E +P + A G ++ATG P Q NN+L FPG
Sbjct: 506 HPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPG 565
Query: 327 IFRGALDVRARSITDDMIIEAAKAIASVVEEPSE-ENIIPSPFN-PIVYAKEARAVAEEA 384
I G + AR IT M+ AAKAIA + ++++P N + A AV A
Sbjct: 566 IGLGVIVAGARLITRRMLHAAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAA 625
Query: 385 MKEGVA 390
+++GVA
Sbjct: 626 VQDGVA 631
>sp|Q48796|MLES_OENOE MALOLACTIC ENZYME >gi|7431227|pir||T13496 malolactic enzyme (EC
1.1.1.-) - Leuconostoc oenos >gi|1359914|emb|CAA57769.1|
(X82326) malolactic enzyme [Oenococcus oeni]
Length = 541
Score = 94.0 bits (230), Expect = 3e-18
Identities = 103/409 (25%), Positives = 183/409 (44%), Gaps = 67/409 (16%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAFPIMIGE-----------QDP- 107
VVSD ILG+G+ G + G+ + GK +++ GID ++ +DP
Sbjct: 140 VVSDAEGILGIGDWG-VQGVDIAVGKLMVYTVAAGIDPSTVLAVVIDAGTNNEKLLKDPM 198
Query: 108 ------------------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPV 149
DKF+ +++ P ++ ED IL+ +++ I
Sbjct: 199 YLGNKFNRVRGDKYYDFIDKFVNHAESLFPNLY-LHWEDFGRSNASNILNSYKDK--IAT 255
Query: 150 FHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG---- 205
F+DD QGT VVLAG++ AL++ G++L + + FGAG AG ++ L + V+ G
Sbjct: 256 FNDDIQGTGIVVLAGVLGALKISGQKLTDQTYMSFGAGTAGMGIVKQLHEEMVEQGLSDE 315
Query: 206 -NVRVVELVNGKPMVLTKDLDL---EKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLIS 261
+ LV+ + ++ D DL +K F + + N EA+ ++
Sbjct: 316 EAKKHFFLVDKQGLLFDDDPDLTPEQKPFAAKRSDFKNANQLTNLQAAVEAVH--PTILV 373
Query: 262 FTRPGPGVIKPEWIREMS---DDPIVFPLANP--VPEILPDEAKK--AGARIVAT----- 309
T P E +++MS + PI+FP++NP + E ++ K G ++ T
Sbjct: 374 GTSTHPNSFTEEIVKDMSGYTERPIIFPISNPTKLAEAKAEDVLKWSNGKALIGTGVPVD 433
Query: 310 -----GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEENII 364
G + Q NN L +PG+ GA+ +++ +T +MI AA ++ +V+ +
Sbjct: 434 DIEYEGNAYQIGQANNALIYPGLGFGAIAAQSKLLTPEMISAAAHSLGGIVDTTKVGAAV 493
Query: 365 PSPFNPIVYAKE--ARAVAEEAMKEGVARVKVKGEEIEERTLKLISLWD 411
P + + A AVA++A+++G+ R + E LK WD
Sbjct: 494 LPPVSKLADFSRTVAVAVAKKAVEQGLNRQPIDDVEKAVDDLK----WD 538
>sp|P40375|MAOX_SCHPO NAD-DEPENDENT MALIC ENZYME (NAD-ME) >gi|629900|pir||S44330 malate
oxidoreductase nad - fission yeast (Schizosaccharomyces
pombe) >gi|499096|gb|AAA18985.1| (U00621) L-malate:NAD+
oxidoreductase (oxaloacetate-decarboxylating); malic
enzyme, NAD+-binding [Schizosaccharomyces pombe]
>gi|3150125|emb|CAA19139.1| (AL023595) malate
oxidoreductase [nad] [Schizosaccharomyces pombe]
Length = 565
Score = 92.4 bits (226), Expect = 8e-18
Identities = 109/408 (26%), Positives = 175/408 (42%), Gaps = 84/408 (20%)
Query: 60 VVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDA---FPIMI-------------- 102
+++D ILG+G+ G + G+ + K L G+D PI++
Sbjct: 153 IITDSEGILGIGDQG-VGGVLISVAKGHLMTLCAGLDPNRFLPIVLDVGTNNETHRKNHQ 211
Query: 103 ----------GEQDPDKFIEVVKAISPTF--GGINLEDIASPKCFYILDKLREELDIPVF 150
GEQ V+KAI F I+ ED ILD R DI F
Sbjct: 212 YMGLRKDRVRGEQYDSFLDNVIKAIREVFPEAFIHFEDFGLANAKRILDHYRP--DIACF 269
Query: 151 HDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKP------ 204
+DD QGT AV LA +I AL V L E + +FGAG AG +V V
Sbjct: 270 NDDIQGTGAVALAAIIGALHVTKSPLTEQRIMIFGAGTAGVGIANQIVAGMVTDGLSLDK 329
Query: 205 --GNVRVVE------------LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPE 250
GN+ +++ +G+ L KD D +++ + D +E
Sbjct: 330 ARGNLFMIDRCGLLLERHAKIATDGQKPFLKKDSDFKEV---------PSGDINLESA-- 378
Query: 251 EALKGADVLISFTRPGPGVIKPEWIREMS---DDPIVFPLANP--VPEILPDEAKK--AG 303
AL +L+ + PG + IREMS + PI+FP++NP + E PD+ K G
Sbjct: 379 IALVKPTILLGCSGQ-PGKFTEKAIREMSKHVERPIIFPISNPTTLMEAKPDQIDKWSDG 437
Query: 304 ARIVATGRSDYP----------NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS 353
++ATG P +Q NN L +P + + R + ++D M+ A+ A+A+
Sbjct: 438 KALIATGSPLPPLNRNGKKYVISQCNNALLYPALGVACVLSRCKLLSDGMLKAASDALAT 497
Query: 354 VVEE--PSEENIIPSPFNPIVYAKE-ARAVAEEAMKEGVARVKVKGEE 398
V ++E ++P N ++ AV ++A+ EG++ V + ++
Sbjct: 498 VPRSLFAADEALLPDLNNAREISRHIVFAVLKQAVSEGMSTVDLPKDD 545
>pir||T07135 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) - tomato (fragment) >gi|804817|gb|AAA66051.1|
(L36647) malic enzyme [Lycopersicon esculentum]
Length = 400
Score = 90.5 bits (221), Expect = 3e-17
Identities = 104/382 (27%), Positives = 166/382 (43%), Gaps = 65/382 (17%)
Query: 66 RILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI-------------------- 102
RILGLG++G G+ + GK L+ GG+ PI I
Sbjct: 1 RILGLGDLG-CQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNEKLLNDEFYIGLRQ 59
Query: 103 ----GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQ 155
G++ D E + A+ +G + ED A+ F +L K R VF+DD Q
Sbjct: 60 RRATGQEYYDFLHEFMSAVKQNYGEKILVQFEDFANHNAFELLAKYRNSH--LVFNDDIQ 117
Query: 156 GTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE---- 211
GTA+VVLAGLI +L+++G L + + GAG G ++ N+ V E
Sbjct: 118 GTASVVLAGLIASLKLLGGALCDHTFLFLGAGERGTGIAELIALEISNKTNIPVEETRKK 177
Query: 212 --LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGV 269
LV+ K ++++ + + F + W E + +A+K + G G
Sbjct: 178 IWLVDSKGLIVSGRKETLQAFK-KPWAHEHEPVNNL----LDAVKAVKPTVLIGTSGTGR 232
Query: 270 IKPEWIRE----MSDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GR 311
+ + E M+ P++ L+NP E +EA G + A+ G+
Sbjct: 233 TFTKEVVEAMACMNKRPLIMALSNPTSQAECTAEEAYSWSEGRAVFASGSPFPSFEYDGK 292
Query: 312 SDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIAS-VVEEPSEENIIPSPFNP 370
+ P Q NN FPG G + + DDM++ A++A+A+ V EE + +I PF
Sbjct: 293 LNIPGQANNCYIFPGFGFGLVMSGTIRVDDDMLLAASEALAAQVTEEHYAKGMIYPPFAD 352
Query: 371 I--VYAKEARAVAEEAMKEGVA 390
I + A A +VA +A + GVA
Sbjct: 353 IRKISAHIAASVARKAYELGVA 374
>gb|AAF43601.1|AF187998_1 (AF187998) malic enzyme [Drosophila melanogaster]
Length = 763
Score = 90.1 bits (220), Expect = 4e-17
Identities = 123/442 (27%), Positives = 188/442 (41%), Gaps = 82/442 (18%)
Query: 26 LSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSRIL 68
+ L YTP V C+ + KD +Y+ + VV+DG RIL
Sbjct: 301 MPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERIL 360
Query: 69 GLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMIGE--------QDP--------- 107
GLG++G G+PV GK L+ GI PI + +DP
Sbjct: 361 GLGDLGANGMGIPV--GKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERR 418
Query: 108 ------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
D+FI E + A FG I ED A+ F +L K R+ F+DD QGT
Sbjct: 419 ATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFC--TFNDDIQGT 476
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKA----GVKPGNVRVVELV 213
A+V +AGL+ +L++ +L + ++ GAG A + + A G+ + +
Sbjct: 477 ASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWM 536
Query: 214 NGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK-GADVLISFTRPGPGVIKP 272
V+T+D L ++ L E I+ E K +VLI G G
Sbjct: 537 VDSRGVITRDRPKGGLTEHK--LHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQG-GAFNQ 593
Query: 273 EWIREMSD---DPIVFPLANPV--PEILPDEA--KKAGARIVATG----------RSDYP 315
E + M+D PI+F L+NP E +EA G I A+G + YP
Sbjct: 594 EILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYP 653
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPSPFNPIVY 373
Q NN FPG+ G L +I + + + AA+ +A +V ++ ++ ++ P P + IV
Sbjct: 654 GQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYP-PLSSIVS 712
Query: 374 AKEARA--VAEEAMKEGVARVK 393
A A + E A K G+A V+
Sbjct: 713 CSMAIAERIVEYAYKNGLATVR 734
>gb|AAF43602.1|AF187999_1 (AF187999) malic enzyme [Drosophila melanogaster]
Length = 759
Score = 90.1 bits (220), Expect = 4e-17
Identities = 123/442 (27%), Positives = 188/442 (41%), Gaps = 82/442 (18%)
Query: 26 LSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSRIL 68
+ L YTP V C+ + KD +Y+ + VV+DG RIL
Sbjct: 297 MPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERIL 356
Query: 69 GLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMIGE--------QDP--------- 107
GLG++G G+PV GK L+ GI PI + +DP
Sbjct: 357 GLGDLGANGMGIPV--GKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERR 414
Query: 108 ------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
D+FI E + A FG I ED A+ F +L K R+ F+DD QGT
Sbjct: 415 ATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFC--TFNDDIQGT 472
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKA----GVKPGNVRVVELV 213
A+V +AGL+ +L++ +L + ++ GAG A + + A G+ + +
Sbjct: 473 ASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWM 532
Query: 214 NGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK-GADVLISFTRPGPGVIKP 272
V+T+D L ++ L E I+ E K +VLI G G
Sbjct: 533 VDSRGVITRDRPKGGLTEHK--LHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQG-GAFNQ 589
Query: 273 EWIREMSD---DPIVFPLANPV--PEILPDEA--KKAGARIVATG----------RSDYP 315
E + M+D PI+F L+NP E +EA G I A+G + YP
Sbjct: 590 EILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYP 649
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPSPFNPIVY 373
Q NN FPG+ G L +I + + + AA+ +A +V ++ ++ ++ P P + IV
Sbjct: 650 GQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYP-PLSSIVS 708
Query: 374 AKEARA--VAEEAMKEGVARVK 393
A A + E A K G+A V+
Sbjct: 709 CSMAIAERIVEYAYKNGLATVR 730
>gb|AAF43603.1|AF188000_1 (AF188000) malic enzyme [Drosophila melanogaster]
Length = 578
Score = 90.1 bits (220), Expect = 4e-17
Identities = 123/442 (27%), Positives = 188/442 (41%), Gaps = 82/442 (18%)
Query: 26 LSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSRIL 68
+ L YTP V C+ + KD +Y+ + VV+DG RIL
Sbjct: 116 MPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERIL 175
Query: 69 GLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMIGE--------QDP--------- 107
GLG++G G+PV GK L+ GI PI + +DP
Sbjct: 176 GLGDLGANGMGIPV--GKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERR 233
Query: 108 ------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
D+FI E + A FG I ED A+ F +L K R+ F+DD QGT
Sbjct: 234 ATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFC--TFNDDIQGT 291
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKA----GVKPGNVRVVELV 213
A+V +AGL+ +L++ +L + ++ GAG A + + A G+ + +
Sbjct: 292 ASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWM 351
Query: 214 NGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK-GADVLISFTRPGPGVIKP 272
V+T+D L ++ L E I+ E K +VLI G G
Sbjct: 352 VDSRGVITRDRPKGGLTEHK--LHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQG-GAFNQ 408
Query: 273 EWIREMSD---DPIVFPLANPV--PEILPDEA--KKAGARIVATG----------RSDYP 315
E + M+D PI+F L+NP E +EA G I A+G + YP
Sbjct: 409 EILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYP 468
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPSPFNPIVY 373
Q NN FPG+ G L +I + + + AA+ +A +V ++ ++ ++ P P + IV
Sbjct: 469 GQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYP-PLSSIVS 527
Query: 374 AKEARA--VAEEAMKEGVARVK 393
A A + E A K G+A V+
Sbjct: 528 CSMAIAERIVEYAYKNGLATVR 549
>gb|AAF54859.1| (AE003697) Men gene product [alt 1] [Drosophila melanogaster]
Length = 763
Score = 90.1 bits (220), Expect = 4e-17
Identities = 123/442 (27%), Positives = 188/442 (41%), Gaps = 82/442 (18%)
Query: 26 LSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSRIL 68
+ L YTP V C+ + KD +Y+ + VV+DG RIL
Sbjct: 301 MPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERIL 360
Query: 69 GLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMIGE--------QDP--------- 107
GLG++G G+PV GK L+ GI PI + +DP
Sbjct: 361 GLGDLGANGMGIPV--GKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERR 418
Query: 108 ------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
D+FI E + A FG I ED A+ F +L K R+ F+DD QGT
Sbjct: 419 ATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFC--TFNDDIQGT 476
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKA----GVKPGNVRVVELV 213
A+V +AGL+ +L++ +L + ++ GAG A + + A G+ + +
Sbjct: 477 ASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWM 536
Query: 214 NGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK-GADVLISFTRPGPGVIKP 272
V+T+D L ++ L E I+ E K +VLI G G
Sbjct: 537 VDSRGVITRDRPKGGLTEHK--LHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQG-GAFNQ 593
Query: 273 EWIREMSD---DPIVFPLANPV--PEILPDEA--KKAGARIVATG----------RSDYP 315
E + M+D PI+F L+NP E +EA G I A+G + YP
Sbjct: 594 EILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYP 653
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPSPFNPIVY 373
Q NN FPG+ G L +I + + + AA+ +A +V ++ ++ ++ P P + IV
Sbjct: 654 GQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYP-PLSSIVS 712
Query: 374 AKEARA--VAEEAMKEGVARVK 393
A A + E A K G+A V+
Sbjct: 713 CSMAIAERIVEYAYKNGLATVR 734
>gb|AAF54860.1| (AE003697) Men gene product [alt 2] [Drosophila melanogaster]
Length = 759
Score = 90.1 bits (220), Expect = 4e-17
Identities = 123/442 (27%), Positives = 188/442 (41%), Gaps = 82/442 (18%)
Query: 26 LSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSRIL 68
+ L YTP V C+ + KD +Y+ + VV+DG RIL
Sbjct: 297 MPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERIL 356
Query: 69 GLGNIGPLA-GLPVMEGKALLFKRFGGI---DAFPIMIGE--------QDP--------- 107
GLG++G G+PV GK L+ GI PI + +DP
Sbjct: 357 GLGDLGANGMGIPV--GKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERR 414
Query: 108 ------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGT 157
D+FI E + A FG I ED A+ F +L K R+ F+DD QGT
Sbjct: 415 ATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFC--TFNDDIQGT 472
Query: 158 AAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKA----GVKPGNVRVVELV 213
A+V +AGL+ +L++ +L + ++ GAG A + + A G+ + +
Sbjct: 473 ASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWM 532
Query: 214 NGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALK-GADVLISFTRPGPGVIKP 272
V+T+D L ++ L E I+ E K +VLI G G
Sbjct: 533 VDSRGVITRDRPKGGLTEHK--LHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQG-GAFNQ 589
Query: 273 EWIREMSD---DPIVFPLANPV--PEILPDEA--KKAGARIVATG----------RSDYP 315
E + M+D PI+F L+NP E +EA G I A+G + YP
Sbjct: 590 EILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYP 649
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPSPFNPIVY 373
Q NN FPG+ G L +I + + + AA+ +A +V ++ ++ ++ P P + IV
Sbjct: 650 GQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYP-PLSSIVS 708
Query: 374 AKEARA--VAEEAMKEGVARVK 393
A A + E A K G+A V+
Sbjct: 709 CSMAIAERIVEYAYKNGLATVR 730
>gi|5729920 malic enzyme 3, NADP(+)-dependent, mitochondrial; malic enzyme,
NADP+-dependent, mitochondrial [Homo sapiens]
>gi|2497786|sp|Q16798|MAON_HUMAN NADP-DEPENDENT MALIC
ENZYME, MITOCHONDRIAL PRECURSOR (NADP-ME)
>gi|1082582|pir||S53351 malate dehydrogenase
(oxaloacetate-decarboxylating) (NADP+) (EC 1.1.1.40)
precursor, mitochondrial - human
>gi|535012|emb|CAA55956.1| (X79440) NADP+-dependent
malic enzyme [Homo sapiens]
Length = 604
Score = 88.2 bits (215), Expect = 2e-16
Identities = 95/354 (26%), Positives = 153/354 (42%), Gaps = 67/354 (18%)
Query: 60 VVSDGSRILGLGNIGPLA-GLPVMEGKALLFKRFGGID---AFPIMI------------- 102
VV+DG RILGLG++G G+PV GK L+ GG++ P+++
Sbjct: 184 VVTDGERILGLGDLGCYGMGIPV--GKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDP 241
Query: 103 -----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIP 148
G+ D E ++A++ FG I ED A+ F +L+K R + +
Sbjct: 242 LYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCM- 300
Query: 149 VFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG--- 205
F+DD QGTA+V +AG++ AL + +L GAG A +LV A K G
Sbjct: 301 -FNDDIQGTASVAVAGILAALRITNNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPK 359
Query: 206 --NVRVVELVNGKPMVL--TKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLIS 261
R + +V+ K +++ L+ EK + + +E + A+ G +
Sbjct: 360 AEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAI-- 417
Query: 262 FTRPGPGVIKPEWIREMS---DDPIVFPLANPVPEILPDEAK----KAGARIVAT----- 309
G + +R+M+ + PI+F L+NP + K G I A+
Sbjct: 418 -----AGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFK 472
Query: 310 ------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEE 357
G++ P Q NN FPG+ G + R I D++ + A+ IA V E
Sbjct: 473 SVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSE 526
>pir||S69778 adhesin AP65-1 precursor - Trichomonas vaginalis
>gi|687630|gb|AAA87406.1| (U18346) AP65-1 adhesin
[Trichomonas vaginalis]
Length = 567
Score = 88.2 bits (215), Expect = 2e-16
Identities = 115/425 (27%), Positives = 178/425 (41%), Gaps = 90/425 (21%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E + + YTP V E C+ A +D K+ + Y + VV+D R
Sbjct: 106 ETMPILYTPTVGEACQKWATHRQSYRGIYITPEDSGKIKDILRNYPRQDIRCIVVTDAGR 165
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID-------------------AFPIMIGEQDP 107
ILGLG++G +GL + GK +L+ G +D A P+ G + P
Sbjct: 166 ILGLGDLGA-SGLGIPVGKLMLYTLIGQVDPDQTLPVQLDMGTDRKEILADPLYHGWRHP 224
Query: 108 D-------KFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
KF+ E V+A+ FG + ED F +LD R + F+DD +G
Sbjct: 225 RVRGAEHLKFVTEFVEAVKEVFGDTCLVQFEDFEMETAFKLLDHFRWRCNC--FNDDIEG 282
Query: 157 TAAVVLAGLINALEVVG-KRLDEISVALFGAGAAGFATLRILVKAGVKPGNV------RV 209
TAAV A L +A + G L + GAG+A ++V V G + +
Sbjct: 283 TAAVAAATLASATHMEGVPDLKNQKIIFIGAGSAAIGIANLIVDMTVSRGGITKEQAFKN 342
Query: 210 VELVNGKPMVLT--KDL-DLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPG 266
+ + + + MV KDL D K PY +D + G EA+K G
Sbjct: 343 IIMFDHRGMVHAGRKDLYDFNK--PY-------MHDMEVYGSVLEAVKKFKATCVIGVSG 393
Query: 267 -PGVIKPEWIR---EMSDDPIVFPLANPVP--EILPDEAK--KAGARIVAT--------- 309
PG+I E ++ + ++ P++ PL+NP P E P + G + AT
Sbjct: 394 VPGLITKEIVQATLKNAEHPVIMPLSNPTPKAEATPHDVYLWSNGKALCATGSPFPAEQV 453
Query: 310 -GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPS 366
GR Q NN FP + + +AR + AA+++AS+V E+ + N++P
Sbjct: 454 NGRKVITAQANNSWIFPAVGYALVTTKARHCPAKVFEIAAESLASLVKKEDHDQGNLLP- 512
Query: 367 PFNPI 371
P N I
Sbjct: 513 PLNKI 517
>gb|AAA92715.1| (U16837) malic enzyme subunit B proprotein [Trichomonas vaginalis]
>gi|1589187|prf||2210351B malate dehydrogenase:SUBUNIT=B
[Trichomonas vaginalis]
Length = 567
Score = 88.2 bits (215), Expect = 2e-16
Identities = 114/423 (26%), Positives = 174/423 (40%), Gaps = 86/423 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E + + YTP V E C+ A +D K+ + Y + VV+D R
Sbjct: 106 ETMPVLYTPTVGEACQKWATHRQSYRGIYITPEDSGKIKDILRNYPRQDIRCIVVTDAGR 165
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID-------------------AFPIMIGEQDP 107
ILGLG++G +GL + GK +L+ G +D A P+ G + P
Sbjct: 166 ILGLGDLGA-SGLGIPVGKLMLYTLIGQVDPDQTLPVQLDMGTDRKEILADPLYHGWRHP 224
Query: 108 D-------KFIE-VVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
KF+E V A+ FG + ED F +LD R + F+DD +G
Sbjct: 225 RVRGAEHLKFVEEFVAAVKEVFGETCLVQFEDFEMEAAFKLLDHFRWRCNC--FNDDIEG 282
Query: 157 TAAVVLAGLINALEVVG-KRLDEISVALFGAGAAGFATLRILVKAGVKPGNV------RV 209
TAAV A L +A + G L + GAG+A ++V V G + +
Sbjct: 283 TAAVAAATLASATHMEGVPDLKNQKIIFIGAGSAATGIANLIVDMAVSRGGITKEQAYKN 342
Query: 210 VELVNGKPMVLTKDLDLEKL-FPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPG-P 267
+ + + K MV + DL PY +D G EA+K G P
Sbjct: 343 IIMFDHKGMVHSGRTDLYDFNIPY-------MHDMEHYGSVLEAVKKFKATCVIGVSGVP 395
Query: 268 GVIKPEWIR---EMSDDPIVFPLANPVP--EILPDEAK--KAGARIVAT----------G 310
G+I E ++ E ++ P++ PL+NP P E P + G + AT G
Sbjct: 396 GLITKEIVQATLENAEHPVIMPLSNPTPKAEATPHDVYLWSNGKALCATGSPFPAEQVNG 455
Query: 311 RSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVV--EEPSEENIIPSPF 368
R Q NN FP + + +AR + AA+++AS+V E+ + N++P P
Sbjct: 456 RKIITAQANNSWIFPAVGYALVTTQARHCPPKVFEIAAESLASLVKKEDHDQGNLLP-PL 514
Query: 369 NPI 371
N I
Sbjct: 515 NKI 517
>pir||D69655 malate dehydrogenase malS - Bacillus subtilis
>gi|2293209|gb|AAC00287.1| (AF008220) putative
malolactic enzyme [Bacillus subtilis]
>gi|2635472|emb|CAB14966.1| (Z99119) malate
dehydrogenase (decarboxylating) [Bacillus subtilis]
Length = 566
Score = 88.2 bits (215), Expect = 2e-16
Identities = 111/412 (26%), Positives = 177/412 (42%), Gaps = 72/412 (17%)
Query: 43 KDPEKVYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF---- 98
K+ K Y+ S + V +D ILG+G+ G + G+ + GK ++ GID
Sbjct: 137 KEAFKQYKDQSDTIDLIVATDAEGILGIGDWG-VGGIAISIGKLAVYTAAAGIDPSRVLA 195
Query: 99 ---------------PIMIGEQDP-------DKFIEVVKAIS-PTFGG--INLEDIASPK 133
P+ +G Q D+FI+ A++ TF ++ ED +
Sbjct: 196 VVLDAGTNQESLLNDPLYVGNQHSRVRGERYDQFIDDYVALARETFPNALLHWEDFGAKN 255
Query: 134 CFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFAT 193
IL + +++ + F+DD QGT AV LA +++ + L + V +FGAG AG
Sbjct: 256 ARSILKRYKDK--VCTFNDDIQGTGAVSLAAVLSCAKASKVPLRDHRVVIFGAGTAGIGI 313
Query: 194 LRILVKAGVKPGNVRVVELVN----GKPMVLTKDLD--LEKLFPYRGWLLE-KTNDEGIE 246
L +A V+ G + +LT D+D L+ PY E K +
Sbjct: 314 AEQLREALVREGLSEEESYKRFWCIDRNGLLTDDMDQLLDFQKPYARSADEVKDYQRNGD 373
Query: 247 GGPEEAL----KGADVLISFTRPGPGVIKPEWIREMSDD---PIVFPLANP--VPEILPD 297
GG + L + ++ T G E ++EM+ P + P++NP + E P+
Sbjct: 374 GGGIDLLEVVRQAKPTILIGTSTVSGAFTEEIVKEMASHVKRPAILPMSNPTTLSEAKPE 433
Query: 298 EAKK--AGARIVATGRSDYP-----------NQINNLLGFPGIFRGALDVRARSITDDMI 344
+ + G ++ TG S +P Q NN L FPG+ G + +++ ITD M
Sbjct: 434 DLIEWTEGRALITTG-SPFPPVEYNGVTYHIGQANNALVFPGLGLGTIVTKSKLITDGMF 492
Query: 345 IEAAKAIASVVEEPSEENIIPSPFNP------IVYAKEARAVAEEAMKEGVA 390
A+AIA +V + +P P V A A VA+ AMKEGVA
Sbjct: 493 EACARAIAGMV----NVGVPGAPMLPKVEDLRTVSATVAVEVAKTAMKEGVA 540
>sp|P37224|MAOM_AMAHP NAD-DEPENDENT MALIC ENZYME 65 KDA ISOFORM, MITOCHONDRIAL PRECURSOR
(NAD-ME) >gi|1076271|pir||A49983 malate dehydrogenase
(decarboxylating) (EC 1.1.1.39) precursor, mitochondrial
- prince's feather >gi|437104|gb|AAA19014.1| (U01162) C4
photosynthetic NAD-dependent malic enzyme subunit alpha
precursor [Amaranthus hypochondriacus]
Length = 623
Score = 87.8 bits (214), Expect = 2e-16
Identities = 112/411 (27%), Positives = 180/411 (43%), Gaps = 73/411 (17%)
Query: 48 VYEYTSKGNSVAVVSDGSRILGLGNIGPLAGLPVMEGKALLFKRFGGID---AFPIMI-- 102
VY + ++ + VV+DGSRILGLG++G + G+ V GK L+ GI+ P+MI
Sbjct: 178 VYNWPAEQVDMIVVTDGSRILGLGDLG-VHGIGVAIGKLDLYVAAAGINPQRVLPVMIDV 236
Query: 103 ----------------------GEQD---PDKFIEVVKAISPTFGGINLEDIASPKCFYI 137
GE+ D+F+E V P + EDI + +
Sbjct: 237 GTNNEDLLKNPLYLGLQKKRLDGEEYLAVMDEFMEAVFTRWPNVI-VQFEDIQNKWALTL 295
Query: 138 LDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEI---SVALFGAGAAGFATL 194
L + R + F+ D QGT+ V +AGL+ A+ G+ + + + + GAG++G L
Sbjct: 296 LQRYRHKYR--TFNVDVQGTSGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSSGVGVL 353
Query: 195 RILVKAGVKP-GNVRVVE--------LVNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGI 245
K + GN +V+ K ++ K +L+ W + + +G+
Sbjct: 354 NAARKTMARMLGNDESAFDRARSQFWVVDDKGLITEKRANLDPEVQPFAWKENEISLQGL 413
Query: 246 EGGPE--EALKGA--DVLISFTRPGPGVIKPEWIREMSDD----PIVFPLANPVP--EIL 295
G + E ++ DVL+ + G G+ E + + D P +F ++NP E
Sbjct: 414 NEGAKLVEVVRQVKPDVLLGLSAYG-GLFSKEVLEALKDSTSTRPAIFAMSNPTKNAECT 472
Query: 296 PDEAKK-AGARIV------------ATGRSDYPNQINNLLGFPGIFRGALDVRARSITDD 342
P+EA G +V G+ + NQ NN+ FPGI G L +R I+D
Sbjct: 473 PEEAFSIVGDHVVYASGSPFKDVDLGNGKIGHVNQGNNMYLFPGIGLGVLLSGSRIISDS 532
Query: 343 MIIEAAKAIASVV--EEPSEENIIPSPFNPIVYAKE-ARAVAEEAMKEGVA 390
M AA+ +A + EE I PS KE A AV +EA++E +A
Sbjct: 533 MFQAAAERLAGYMTDEEVINGVIYPSISRIRDITKEVAAAVIKEAVEEDLA 583
>pir||S17455 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) (EC
1.1.1.40) - Flaveria linearis (fragment)
>gi|1679884|gb|AAB41026.1| (M59415) NADP-malic enzyme
[Flaveria linearis]
Length = 347
Score = 87.4 bits (213), Expect = 3e-16
Identities = 95/318 (29%), Positives = 146/318 (45%), Gaps = 37/318 (11%)
Query: 103 GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGTAA 159
G++ + E + A+ +G I ED A+ F +L+K R VF+DD QGTA+
Sbjct: 11 GKEYAELMNEFMSAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTH--LVFNDDIQGTAS 68
Query: 160 VVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE------LV 213
VVLAGLI+AL++VG L + GAG AG ++ K N + E LV
Sbjct: 69 VVLAGLISALKLVGGSLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLV 128
Query: 214 NGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKG--ADVLISFTRPGPGVIK 271
+ K +++ LD + F + W +D +A+K VLI + G K
Sbjct: 129 DSKGLIVRSRLDSLQHFK-KPW----AHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTK 183
Query: 272 P--EWIREMSDDPIVFPLANPV--PEILPDEAK--KAGARIVATGRSDYP---------- 315
E +R ++ PI+ L+NP E ++A G I A+G P
Sbjct: 184 EVVEAMRSFNEKPIILALSNPTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVS 243
Query: 316 NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI--V 372
Q NN FPG G + A + D+M++ A++A+A V +E + +I PF I +
Sbjct: 244 GQSNNAYIFPGFGLGLIISGAIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKI 303
Query: 373 YAKEARAVAEEAMKEGVA 390
A A VA +A + G+A
Sbjct: 304 SAHIAAKVAAKAYELGLA 321
>gi|11438058 malic enzyme 3, NADP(+)-dependent, mitochondrial [Homo sapiens]
Length = 542
Score = 87.4 bits (213), Expect = 3e-16
Identities = 95/354 (26%), Positives = 153/354 (42%), Gaps = 67/354 (18%)
Query: 60 VVSDGSRILGLGNIGPLA-GLPVMEGKALLFKRFGGID---AFPIMI------------- 102
VV+DG RILGLG++G G+PV GK L+ GG++ P+++
Sbjct: 122 VVTDGERILGLGDLGCYGMGIPV--GKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDP 179
Query: 103 -----------GEQDPDKFIEVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIP 148
G+ D E ++A++ FG I ED A+ F +L+K R + +
Sbjct: 180 LYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCM- 238
Query: 149 VFHDDQQGTAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPG--- 205
F+DD QGTA+V +AG++ AL + +L GAG A +LV A K G
Sbjct: 239 -FNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPK 297
Query: 206 --NVRVVELVNGKPMVL--TKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLIS 261
R + +V+ K +++ L+ EK + + +E + A+ G +
Sbjct: 298 AEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAI-- 355
Query: 262 FTRPGPGVIKPEWIREMS---DDPIVFPLANPVPEILPDEAK----KAGARIVAT----- 309
G + +R+M+ + PI+F L+NP + K G I A+
Sbjct: 356 -----AGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFK 410
Query: 310 ------GRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEE 357
G++ P Q NN FPG+ G + R I D++ + A+ IA V E
Sbjct: 411 SVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSE 464
>emb|CAB52344.1| (AL049699) dJ747H23.1 (malic enzyme 1, soluble (NADP-dependent
malic enzyme, malate oxidoreductase, EC 1.1.1.40)) [Homo
sapiens]
Length = 337
Score = 87.0 bits (212), Expect = 4e-16
Identities = 81/303 (26%), Positives = 135/303 (43%), Gaps = 49/303 (16%)
Query: 124 INLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINALEVVGKRLDEISVAL 183
I ED A+ F +L+K R + F+DD QGTA+V +AGL+ AL + +L + ++
Sbjct: 7 IQFEDFANVNAFRLLNKYRNQYC--TFNDDIQGTASVAVAGLLAALRITKNKLSDQTILF 64
Query: 184 FGAGAAGFATLRILVKAGVKPG-----NVRVVELVNGKPMVLTKDLDLEKLFPYRGWLLE 238
GAG A ++V A K G ++ + LV+ K +++ L
Sbjct: 65 QGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASL------------ 112
Query: 239 KTNDEGIEGGPEEALKGADVLISFTRPGP--------GVIKPEWIREM---SDDPIVFPL 287
T ++ E +K + ++ +P G + +++M ++ PI+F L
Sbjct: 113 -TQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFAL 171
Query: 288 ANPV--PEILPDEAKK--AGARIVAT-----------GRSDYPNQINNLLGFPGIFRGAL 332
+NP E ++ K G I A+ G++ YP Q NN FPG+ G +
Sbjct: 172 SNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVV 231
Query: 333 DVRARSITDDMIIEAAKAIASVVEEPS-EENIIPSPFNPI--VYAKEARAVAEEAMKEGV 389
R ITD++ + A+ IA V + EE + P N I V K A + ++A +E
Sbjct: 232 ACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKT 291
Query: 390 ARV 392
A V
Sbjct: 292 ATV 294
>gb|AAF68116.1|AC010793_11 (AC010793) F20B17.18 [Arabidopsis thaliana]
Length = 663
Score = 82.7 bits (201), Expect = 7e-15
Identities = 90/320 (28%), Positives = 146/320 (45%), Gaps = 37/320 (11%)
Query: 112 EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVVLAGLINA 168
E + A+ +G I ED A+ F +L K VF+DD QGTA+VVLAGLI A
Sbjct: 336 EFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTH--LVFNDDIQGTASVVLAGLIAA 393
Query: 169 LEVVGKRLDEISVALFGAGAAGFATLRILVKAGVKPGNVRVVE------LVNGKPMVLTK 222
L VG L + GAG AG ++ K ++ + E LV+ K ++++
Sbjct: 394 LRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSS 453
Query: 223 DLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPGPGVI----KPEWIREM 278
+ + F + W +D +A+K + G G E + ++
Sbjct: 454 RKESIQHFK-KPW----AHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKL 508
Query: 279 SDDPIVFPLANPV--PEILPDEAK--KAGARIVAT----------GRSDYPNQINNLLGF 324
++ PI+ L+NP E +EA G I A+ G++ P Q NN F
Sbjct: 509 NEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIF 568
Query: 325 PGIFRGALDVRARSITDDMIIEAAKAIA-SVVEEPSEENIIPSPFNPI--VYAKEARAVA 381
PG G + + DDM++ A++A+A ++EE E+ +I PF I + A+ A VA
Sbjct: 569 PGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVA 628
Query: 382 EEAMKEGVARVKVKGEEIEE 401
+A + G+A + +E+E+
Sbjct: 629 AKAYELGLATRLPQPKELEQ 648
>gb|AAA91133.1| (U35243) AP65-3 adhesin [Trichomonas vaginalis]
Length = 567
Score = 82.3 bits (200), Expect = 9e-15
Identities = 113/420 (26%), Positives = 173/420 (40%), Gaps = 91/420 (21%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E + + YTP V E C+ A +D K+ + Y + VV+DG R
Sbjct: 106 ETMPVLYTPTVGEACQKWATHRQSYRGIYITPEDSGKIKDILRNYPRQDIRCIVVTDGGR 165
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID-------------------AFPIMIGEQDP 107
ILGLG++G +GL + GK +L+ G + A P+ G + P
Sbjct: 166 ILGLGDLG-ASGLGIPVGKLMLYTLIGQVHPDQTLPVQLDMGTDRKEILADPLYHGWRHP 224
Query: 108 -------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
KF+ E V A+ FG + ED F +LD R + F+DD +G
Sbjct: 225 RIRGPEHTKFVAEFVDAVKEVFGETCLVQFEDFEMETAFKLLDHFRWRCN--CFNDDIEG 282
Query: 157 TAAVVLAGLINALEVVG-KRLDEISVALFGAGAAGFATLRILVKAGVKPGNV------RV 209
TAAV A L +A + G L + GAG+A ++V V G + R
Sbjct: 283 TAAVAAATLASATHMEGVPDLKNQKIIFIGAGSAATGIANLIVDMAVSRGGISRKDAERN 342
Query: 210 VELVNGKPMVLT--KDL-DLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPG 266
+ + + K MV KDL D K PY +D + G E +K G
Sbjct: 343 IIMFDHKGMVHADRKDLYDFNK--PY-------MHDMEVYGSVLEGVKKFKATCVIGVSG 393
Query: 267 -PGVIKPEWIREM---SDDPIVFPLANPV--PEILPDEAKK--AGARIVATGRSDYP--- 315
PG+I E ++ + P++ PL+NP E P + + G + ATG S +P
Sbjct: 394 VPGLITKEIVQATCANCERPVIMPLSNPTVKAEAKPHDVYQWSNGKALCATG-SPFPVET 452
Query: 316 --------NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEE--NIIP 365
Q NN FP + + RAR + AA+++AS+V++ + N++P
Sbjct: 453 VNGKKTITAQANNSWIFPAVGYALVTTRARHCPGKVFEVAAESLASLVKKEDHDMGNLLP 512
>gb|AAB39711.1| (U73780) malic enzyme [Pseudomonas aeruginosa]
Length = 120
Score = 81.5 bits (198), Expect = 2e-14
Identities = 46/101 (45%), Positives = 61/101 (59%), Gaps = 16/101 (15%)
Query: 306 IVATGRSDYPNQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEP------- 358
I+ATGRSDYPNQ+NN+LGFP IFRGALDVRA I ++M I AA A+ + ++P
Sbjct: 1 IMATGRSDYPNQVNNVLGFPFIFRGALDVRATRINEEMKIAAALALRDLAKQPVPEDVCA 60
Query: 359 ---------SEENIIPSPFNPIVYAKEARAVAEEAMKEGVA 390
E IIP P + + + AVA+ A++ GVA
Sbjct: 61 AYGVDKLEFGREYIIPKPMDKRLITVVSDAVAKAAIETGVA 101
>pir||S69779 adhesin AP65-2 precursor - Trichomonas vaginalis
>gi|687632|gb|AAA87407.1| (U18347) AP65-2 adhesin
[Trichomonas vaginalis] >gi|885635|gb|AAA92714.1|
(U16836) malic enzyme subunit A proprotein [Trichomonas
vaginalis] >gi|1589186|prf||2210351A malate
dehydrogenase:SUBUNIT=A [Trichomonas vaginalis]
Length = 567
Score = 78.8 bits (191), Expect = 1e-13
Identities = 113/426 (26%), Positives = 176/426 (40%), Gaps = 92/426 (21%)
Query: 24 EHLSLAYTPGVAEPCKAIA-------------KDPEKVYE----YTSKGNSVAVVSDGSR 66
E + + YTP V E C+ A +D K+ + Y + VV+D R
Sbjct: 106 ETMPVLYTPTVGEACQKWATHRQSYRGIYITPEDSGKIKDILRNYPRQDIRCIVVTDAGR 165
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGID-------------------AFPIMIGEQDP 107
ILGLG++G +GL + GK +L+ G ++ A P+ G + P
Sbjct: 166 ILGLGDLGA-SGLGIPVGKLMLYTLIGQVNPDQTLPVQLDMGTDRKEILADPLYHGWRHP 224
Query: 108 -------DKFI-EVVKAISPTFGG---INLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
KF+ E V A+ FG + ED F +LD R + F+DD +G
Sbjct: 225 RIRGPEHTKFVAEFVDAVKEVFGETCLVQFEDFEMETAFTLLDHFRWRCNC--FNDDIEG 282
Query: 157 TAAVVLAGLINALEVVG-KRLDEISVALFGAGAAGFATLRILVKAGVKPGNV------RV 209
TAAV A L +A + G L + GAG+A ++V V G + +
Sbjct: 283 TAAVAAATLASATHMEGVPDLKNQKIIFIGAGSAATGIANLIVDMAVSRGGITKEQAYKN 342
Query: 210 VELVNGKPMVLT--KDL-DLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADVLISFTRPG 266
+ + + K MV KDL D K PY ++ + G E +K G
Sbjct: 343 IIMFDHKGMVHAGRKDLYDFNK--PYM-------HNMEVYGSVLEGVKKFKATSVIGVSG 393
Query: 267 -PGVIKPEWIREMS---DDPIVFPLANPV--PEILPDEAKK--AGARIVATGRSDYP--- 315
PG+I E ++ + P++ PL+NP E P + + G + ATG S +P
Sbjct: 394 VPGLITKEIVQAACANCERPVIMPLSNPTVKAEAKPHDVYQWSNGKALCATG-SPFPVET 452
Query: 316 --------NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASVVEEPSEE--NIIP 365
Q NN FP + + RAR + AA+++AS+V++ + N++P
Sbjct: 453 VNGKKTITAQANNSWIFPAVGYALVTTRARHCPGKVFEVAAESLASLVKKEDHDMGNLLP 512
Query: 366 SPFNPI 371
P N I
Sbjct: 513 -PLNKI 517
>emb|CAA39419.1| (X55956) alternative [Escherichia coli]
Length = 490
Score = 77.6 bits (188), Expect = 2e-13
Identities = 87/332 (26%), Positives = 136/332 (40%), Gaps = 67/332 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIAKDPEK----VYEYTSKGN-------------SVAVVSDGSR 66
E + + YTP V C+ ++ + Y ++ N V VV+DG R
Sbjct: 107 EMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGER 166
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGEQDP 107
ILGLG+ G + G+ + GK L+ GGI P+ +G ++P
Sbjct: 167 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNP 225
Query: 108 -----------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
D+FI+ VK P + ED A +L++ R E I F+DD QG
Sbjct: 226 RITDDEYYEFVDEFIQAVKQRWPDVL-LQFEDFAQKNAMPLLNRYRNE--ICSFNDDIQG 282
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPGNVRVVEL 212
TAAV + LI A G +L E + GAG+AG +++ + G+ R
Sbjct: 283 TAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVF 342
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADV-------LISFTRP 265
+ + +LT + L P++ L++K + + L DV ++
Sbjct: 343 MVDRFGLLTD--KMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSG 400
Query: 266 GPGVIKPEWIREMSDD---PIVFPLANPVPEI 294
G+ E IREM PIV PL+NP +
Sbjct: 401 QTGLFTEEIIREMHKHCPRPIVMPLSNPTSRV 432
>pir||S38951 sfcA protein - Escherichia coli
Length = 447
Score = 77.6 bits (188), Expect = 2e-13
Identities = 87/332 (26%), Positives = 136/332 (40%), Gaps = 67/332 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIAKDPEK----VYEYTSKGN-------------SVAVVSDGSR 66
E + + YTP V C+ ++ + Y ++ N V VV+DG R
Sbjct: 116 EMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGER 175
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGEQDP 107
ILGLG+ G + G+ + GK L+ GGI P+ +G ++P
Sbjct: 176 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNP 234
Query: 108 -----------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
D+FI+ VK P + ED A +L++ R E I F+DD QG
Sbjct: 235 RITDDEYYEFVDEFIQAVKQRWPDVL-LQFEDFAQKNAMPLLNRYRNE--ICSFNDDIQG 291
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPGNVRVVEL 212
TAAV + LI A G +L E + GAG+AG +++ + G+ R
Sbjct: 292 TAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVF 351
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADV-------LISFTRP 265
+ + +LT + L P++ L++K + + L DV ++
Sbjct: 352 MVDRFGLLTD--KMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSG 409
Query: 266 GPGVIKPEWIREMSDD---PIVFPLANPVPEI 294
G+ E IREM PIV PL+NP +
Sbjct: 410 QTGLFTEEIIREMHKHCPRPIVMPLSNPTSRV 441
>emb|CAA39420.1| (X55956) alternative [Escherichia coli]
Length = 484
Score = 77.6 bits (188), Expect = 2e-13
Identities = 87/332 (26%), Positives = 136/332 (40%), Gaps = 67/332 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIAKDPEK----VYEYTSKGN-------------SVAVVSDGSR 66
E + + YTP V C+ ++ + Y ++ N V VV+DG R
Sbjct: 101 EMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGER 160
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGEQDP 107
ILGLG+ G + G+ + GK L+ GGI P+ +G ++P
Sbjct: 161 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNP 219
Query: 108 -----------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
D+FI+ VK P + ED A +L++ R E I F+DD QG
Sbjct: 220 RITDDEYYEFVDEFIQAVKQRWPDVL-LQFEDFAQKNAMPLLNRYRNE--ICSFNDDIQG 276
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPGNVRVVEL 212
TAAV + LI A G +L E + GAG+AG +++ + G+ R
Sbjct: 277 TAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVF 336
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADV-------LISFTRP 265
+ + +LT + L P++ L++K + + L DV ++
Sbjct: 337 MVDRFGLLTD--KMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSG 394
Query: 266 GPGVIKPEWIREMSDD---PIVFPLANPVPEI 294
G+ E IREM PIV PL+NP +
Sbjct: 395 QTGLFTEEIIREMHKHCPRPIVMPLSNPTSRV 426
>emb|CAA39421.1| (X55956) alternative [Escherichia coli]
Length = 481
Score = 77.6 bits (188), Expect = 2e-13
Identities = 87/332 (26%), Positives = 136/332 (40%), Gaps = 67/332 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIAKDPEK----VYEYTSKGN-------------SVAVVSDGSR 66
E + + YTP V C+ ++ + Y ++ N V VV+DG R
Sbjct: 98 EMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGER 157
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGEQDP 107
ILGLG+ G + G+ + GK L+ GGI P+ +G ++P
Sbjct: 158 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNP 216
Query: 108 -----------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
D+FI+ VK P + ED A +L++ R E I F+DD QG
Sbjct: 217 RITDDEYYEFVDEFIQAVKQRWPDVL-LQFEDFAQKNAMPLLNRYRNE--ICSFNDDIQG 273
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPGNVRVVEL 212
TAAV + LI A G +L E + GAG+AG +++ + G+ R
Sbjct: 274 TAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVF 333
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADV-------LISFTRP 265
+ + +LT + L P++ L++K + + L DV ++
Sbjct: 334 MVDRFGLLTD--KMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSG 391
Query: 266 GPGVIKPEWIREMSDD---PIVFPLANPVPEI 294
G+ E IREM PIV PL+NP +
Sbjct: 392 QTGLFTEEIIREMHKHCPRPIVMPLSNPTSRV 423
>emb|CAA39422.1| (X55956) alternative [Escherichia coli]
Length = 448
Score = 77.6 bits (188), Expect = 2e-13
Identities = 87/332 (26%), Positives = 136/332 (40%), Gaps = 67/332 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIAKDPEK----VYEYTSKGN-------------SVAVVSDGSR 66
E + + YTP V C+ ++ + Y ++ N V VV+DG R
Sbjct: 65 EMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGER 124
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGEQDP 107
ILGLG+ G + G+ + GK L+ GGI P+ +G ++P
Sbjct: 125 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNP 183
Query: 108 -----------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
D+FI+ VK P + ED A +L++ R E I F+DD QG
Sbjct: 184 RITDDEYYEFVDEFIQAVKQRWPDVL-LQFEDFAQKNAMPLLNRYRNE--ICSFNDDIQG 240
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPGNVRVVEL 212
TAAV + LI A G +L E + GAG+AG +++ + G+ R
Sbjct: 241 TAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVF 300
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADV-------LISFTRP 265
+ + +LT + L P++ L++K + + L DV ++
Sbjct: 301 MVDRFGLLTD--KMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSG 358
Query: 266 GPGVIKPEWIREMSDD---PIVFPLANPVPEI 294
G+ E IREM PIV PL+NP +
Sbjct: 359 QTGLFTEEIIREMHKHCPRPIVMPLSNPTSRV 390
>emb|CAA39423.1| (X55956) alternative [Escherichia coli]
Length = 433
Score = 77.6 bits (188), Expect = 2e-13
Identities = 87/332 (26%), Positives = 136/332 (40%), Gaps = 67/332 (20%)
Query: 24 EHLSLAYTPGVAEPCKAIAKDPEK----VYEYTSKGN-------------SVAVVSDGSR 66
E + + YTP V C+ ++ + Y ++ N V VV+DG R
Sbjct: 50 EMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGER 109
Query: 67 ILGLGNIGPLAGLPVMEGKALLFKRFGGIDAF-------------------PIMIGEQDP 107
ILGLG+ G + G+ + GK L+ GGI P+ +G ++P
Sbjct: 110 ILGLGDQG-IGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNP 168
Query: 108 -----------DKFIEVVKAISPTFGGINLEDIASPKCFYILDKLREELDIPVFHDDQQG 156
D+FI+ VK P + ED A +L++ R E I F+DD QG
Sbjct: 169 RITDDEYYEFVDEFIQAVKQRWPDVL-LQFEDFAQKNAMPLLNRYRNE--ICSFNDDIQG 225
Query: 157 TAAVVLAGLINALEVVGKRLDEISVALFGAGAAGFATLRILV----KAGVKPGNVRVVEL 212
TAAV + LI A G +L E + GAG+AG +++ + G+ R
Sbjct: 226 TAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVF 285
Query: 213 VNGKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGGPEEALKGADV-------LISFTRP 265
+ + +LT + L P++ L++K + + L DV ++
Sbjct: 286 MVDRFGLLTD--KMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSG 343
Query: 266 GPGVIKPEWIREMSDD---PIVFPLANPVPEI 294
G+ E IREM PIV PL+NP +
Sbjct: 344 QTGLFTEEIIREMHKHCPRPIVMPLSNPTSRV 375
>gb|AAC49572.1| (U62041) malic enzyme precursor [Neocallimastix frontalis]
Length = 592
Score = 76.5 bits (185), Expect = 5e-13
Identities = 109/400 (27%), Positives = 156/400 (38%), Gaps = 82/400 (20%)
Query: 28 LAYTPGVAEPC----KAIAKDPEKVYEYTSKGN-------------SVAVVSDGSRILGL 70
+ YTP V E C K + + +G V VV+DGSRILGL
Sbjct: 132 IIYTPVVGEACQKFHKIFTQTRGMYFSTADRGQMSAVAANWPYDDVDVIVVTDGSRILGL 191
Query: 71 GNIGPLAGLPVMEGKALLFKRFGGIDA-------------------FPIMIGEQDP---- 107
G++G G+ + GK L+ GGI+ P+ +G Q P
Sbjct: 192 GDLG-AGGMQIPIGKLTLYVCGGGINPRNVLPIVLDVGTNNKELLNDPLYLGMQHPRLQG 250
Query: 108 ---DKFI-EVVKAISPTF--GGINLEDIASPKCFYILDKLREELDIPVFHDDQQGTAAVV 161
F+ E V AI+ F I ED P +L K +++ I +F+DD Q T A+
Sbjct: 251 EEFHAFVDEWVSAITDRFPKAVIQFEDFMMPNALDLLLKYKDQ--ICMFNDDIQSTGAIT 308
Query: 162 LAGLINALEVVGKRLDEISVALF---GAGAAGFATLRILVKAGVKPGNVRVVELVN---- 214
LA ++ + G +I F GAG++G +V V G R
Sbjct: 309 LASVLATMRARGGTFADIKKETFLCLGAGSSGVGVCETIVDCIVAEGATREEAYAQFYMF 368
Query: 215 GKPMVLTKDLDLEKLFPYRGWLLEKTNDEGIEGG--PEEALKG-ADVLISFTRPGPGVIK 271
+L K D L P + + K IEGG P E LK + P +
Sbjct: 369 DHKGLLGKGRD--DLLPSQQVFMRKE----IEGGKTPAELLKKIKPTCLLGLSTCPKLFN 422
Query: 272 PE---WIREMSDDPIVFPLANPV--PEILPDEAKK--AGARIVATGRSDYP--------- 315
E ++ + P +FPL+NP E +EA + G I A+G P
Sbjct: 423 KEMLSYVASYCEKPGIFPLSNPTSRSECTAEEAVEFTDGNLIFASGSPFDPVEWKGKTIQ 482
Query: 316 -NQINNLLGFPGIFRGALDVRARSITDDMIIEAAKAIASV 354
NQ NN FPGI G + RA + + A+ IAS+
Sbjct: 483 TNQCNNSYSFPGIGLGLVSSRATRVPFETFQVCARVIASL 522
>pir||T45208 probable malate dehydrogenase (EC 1.1.1.37) mox [imported] -
Mycobacterium leprae >gi|699147|gb|AAA62913.1| (U15180)
malate oxidoreductase(NAD) [Mycobacterium leprae]
Length = 235
Score = 76.1 bits (184), Expect = 7e-13
Identities = 49/108 (45%), Positives = 67/108 (61%), Gaps = 4/108 (3%)
Query: 278 MSDDPIVFPLANPVPEILPDEAKKAGARIVATGRSDYPNQINNLLGFPGIFRGALDVRAR 337
M+ D IVF L++P PEI P A K A +VA GRSD+ +QIN++L FPG+F GALD
Sbjct: 126 MAPDGIVFVLSDPDPEIHPGVAAKYVA-VVAIGRSDFCSQINSVLVFPGVFCGALDAETS 184
Query: 338 SITDDMIIEAAKAIASVVEEP-SEENIIPSPFNPIVYAKEARAVAEEA 384
IT+ M++ A +AI V + + + I+PS N EA A+A EA
Sbjct: 185 RITEKMMVAAVEAIFCVASDDFTLDRIVPSLLN--FRVGEAGALATEA 230
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.140 0.401
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 162898964
Number of Sequences: 2977
Number of extensions: 7394046
Number of successful extensions: 20352
Number of sequences better than 1.0e-10: 127
Number of HSP's better than 0.0 without gapping: 36
Number of HSP's successfully gapped in prelim test: 91
Number of HSP's that attempted gapping in prelim test: 20006
Number of HSP's gapped (non-prelim): 151
length of query: 422
length of database: 189,106,746
effective HSP length: 56
effective length of query: 366
effective length of database: 155,591,474
effective search space: 56946479484
effective search space used: 56946479484
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 166 (69.1 bits)