BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1843 (soxB-like) DE:sarcosine oxidase, subunit beta (soxB)
(382 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G75122 sarcosine oxidase, chain beta (soxb) PAB1843 - Pyroc... 776 0.0
pir||F71008 probable sarcosine oxidase - Pyrococcus horikoshii >... 745 0.0
pir||B71184 probable sarcosine oxidase - Pyrococcus horikoshii >... 419 e-116
pir||F75144 sarcosine oxidase, chain beta (soxb) PAB0214 - Pyroc... 414 e-114
gi|11497890 sarcosine oxidase, subunit beta (soxB) [Archaeoglobu... 182 8e-45
sp|P40875|SAOX_CORS1 SARCOSINE OXIDASE BETA SUBUNIT (SARCOSINE O... 172 7e-42
sp|Q63342|M2GD_RAT DIMETHYLGLYCINE DEHYDROGENASE, MITOCHONDRIAL ... 152 9e-36
gi|7019365 dimethylglycine dehydrogenase precursor [Homo sapiens... 152 9e-36
gi|11417071 dimethylglycine dehydrogenase precursor [Homo sapiens] 152 9e-36
gb|AAD53398.2|AF095735_1 (AF095735) sarcosine dehydrogenase [Hom... 147 2e-34
gb|AAD32214.1| (AF140745) sarcosine dehydrogenase [Homo sapiens]... 147 2e-34
gb|AAD13137.1| (AF047190) dimethylglycine dehydrogenase-like pro... 146 3e-34
gb|AAK40533.1| Sarcosine oxidase, subunit beta (soxB-like) [Sulf... 146 5e-34
gb|AAD03414.1| (AF067650) sarcosine dehydrogenase [Rattus norveg... 146 5e-34
pir||H82968 sarcosine oxidase beta subunit PA5416 [imported] - P... 144 1e-33
gb|AAF57796.1| (AE003802) CG6385 gene product [Drosophila melano... 140 2e-32
sp|O87388|SAOX_RHIME SARCOSINE OXIDASE BETA SUBUNIT (SARCOSINE O... 139 4e-32
dbj|BAB05760.1| (AP001514) unknown conserved protein [Bacillus h... 133 4e-30
gb|AAD13136.1| (AF047004) dimethylglycine dehydrogenase-like pro... 132 6e-30
gb|AAC48785.1| (AF026954) pyruvate dehydrogenase phosphatase reg... 131 2e-29
gi|8923708 hypothetical protein [Homo sapiens] >gi|11437505|ref|... 125 6e-28
pir||E72254 conserved hypothetical protein - Thermotoga maritima... 124 2e-27
gb|AAG18868.1| (AE004990) sarcosine oxidase; SoxB [Halobacterium... 119 6e-26
gb|AAD53399.2|AF095736_1 (AF095736) unknown [Homo sapiens] 116 5e-25
gi|10955084 agaE [Agrobacterium tumefaciens] >gi|11352852|pir||T... 115 1e-24
gi|6005868 sarcosine dehydrogenase; dimethylglycine dehydrogenas... 114 2e-24
gb|AAF57379.1| (AE003789) CG3270 gene product [Drosophila melano... 114 2e-24
dbj|BAB05153.1| (AP001512) glycine oxidase (sarcosine oxidase) [... 112 8e-24
gb|AAB03674.1| (U62481) dimethylglycine dehydrogenase-like prote... 108 1e-22
pir||B75218 glycerol-3-phosphate dehydrogenase (glpa) PAB0183 - ... 108 2e-22
gb|AAF45943.1| (AE003430) CG3626 gene product [Drosophila melano... 108 2e-22
sp|O31616|GLOX_BACSU GLYCINE OXIDASE >gi|7475812|pir||B69845 sar... 106 6e-22
pir||H83370 hydrogen cyanide synthase HcnC PA2195 [imported] - P... 104 2e-21
gb|AAK42656.1| Glycerol-3-phosphate dehydrogenase chain A (glpA)... 104 2e-21
sp|P43799|GLPA_HAEIN ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENAS... 101 2e-20
emb|CAB63337.1| (AL032631) contains similarity to Pfam domain: P... 101 2e-20
gb|AAC38596.1| (AF053760) hydrogen cyanide synthase HcnC [Pseudo... 99 6e-20
gb|AAG20146.1| (AE005092) glycerol-3-phosphate dehydrogenase cha... 98 1e-19
pir||JC7176 L-amino acid deaminase (EC 3.5.4.-) - Proteus vulgar... 97 4e-19
sp|P13032|GLPA_ECOLI ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENAS... 88 2e-16
gb|AAA83864.1| (M20938) glycerol-3-phosphate dehydrogenase subun... 88 2e-16
pir||H70361 conserved hypothetical protein aq_707 - Aquifex aeol... 87 3e-16
gb|AAA86752.1| (U35383) amino acid deaminase [Proteus mirabilis] 84 2e-15
pir||C82001 D-amino-acid dehydrogenase (EC 1.4.99.1) small subun... 84 2e-15
sp|O32159|YURR_BACSU HYPOTHETICAL 39.4 KD OXIDOREDUCTASE IN HOM-... 83 4e-15
pir||B83078 probable D-amino acid oxidase PA4548 [imported] - Ps... 83 5e-15
sp|P33642|YFIT_PSEAE HYPOTHETICAL 39.5 KD OXIDOREDUCTASE IN FIMT... 83 5e-15
sp|Q52671|SAOX_RHOCA SARCOSINE OXIDASE BETA SUBUNIT (SARCOSINE O... 83 6e-15
pir||A81228 D-amino acid dehydrogenase, small chain NMB0176 [imp... 82 8e-15
pir||S76505 hypothetical protein - Synechocystis sp. (strain PCC... 81 2e-14
gb|AAA96395.1| (U38892) N-terminal and C-terminal ends are simil... 81 2e-14
emb|CAC11717.1| (AL445064) sarcosine oxidase related protein [Th... 79 7e-14
pir||E82422 anaerobic glycerol-3-phosphate dehydrogenase, chain ... 76 5e-13
sp|P40854|SAOX_STRSQ SARCOSINE OXIDASE >gi|80855|pir||JS0671 sar... 75 2e-12
gb|AAC60412.1| (S39464) sarcosine oxidase {EC 1.5.3.1} [Streptom... 75 2e-12
sp|P29011|DADA_ECOLI D-AMINO ACID DEHYDROGENASE SMALL SUBUNIT >g... 74 3e-12
gb|AAG09254.1|AF176664_3 (AF176664) iminodiacetate oxidase [EDTA... 73 4e-12
emb|CAB53548.1| (AJ249196) D-alanine dehydrogenase [Rhizobium le... 73 6e-12
emb|CAC11772.1| (AL445064) glycerol-3-phosphate dehydrogenase re... 69 6e-11
>pir||G75122 sarcosine oxidase, chain beta (soxb) PAB1843 - Pyrococcus abyssi
(strain Orsay) >gi|5458207|emb|CAB49696.1| (AJ248285)
sarcosine oxidase, subunit beta (soxB) [Pyrococcus
abyssi]
Length = 382
Score = 776 bits (1982), Expect = 0.0
Identities = 382/382 (100%), Positives = 382/382 (100%)
Query: 1 MLPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN 60
MLPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN
Sbjct: 1 MLPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN 60
Query: 61 VRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPE 120
VRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPE
Sbjct: 61 VRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPE 120
Query: 121 EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG 180
EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG
Sbjct: 121 EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG 180
Query: 181 EIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP 240
EIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP
Sbjct: 181 EIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP 240
Query: 241 MVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLV 300
MVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLV
Sbjct: 241 MVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLV 300
Query: 301 LRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLP 360
LRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLP
Sbjct: 301 LRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLP 360
Query: 361 VEWYDPYRFERGELRTAALQMG 382
VEWYDPYRFERGELRTAALQMG
Sbjct: 361 VEWYDPYRFERGELRTAALQMG 382
>pir||F71008 probable sarcosine oxidase - Pyrococcus horikoshii
>gi|3257787|dbj|BAA30470.1| (AP000006) 382aa long
hypothetical sarcosine oxidase [Pyrococcus horikoshii]
Length = 382
Score = 745 bits (1902), Expect = 0.0
Identities = 358/382 (93%), Positives = 376/382 (97%)
Query: 1 MLPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN 60
MLPE+SE+VVIGGGIVGV+IAHELAKRG +VTV+EKRFIGSGSTFRCGTGIRQQFNDEAN
Sbjct: 1 MLPEKSEIVVIGGGIVGVTIAHELAKRGEEVTVIEKRFIGSGSTFRCGTGIRQQFNDEAN 60
Query: 61 VRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPE 120
VRVMKRSVELWKKYSEEY FSFKQTGYLFLLYD+EEV+TFKRNIEIQNKFGVPT+LITPE
Sbjct: 61 VRVMKRSVELWKKYSEEYGFSFKQTGYLFLLYDDEEVKTFKRNIEIQNKFGVPTKLITPE 120
Query: 121 EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG 180
EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFA+KA+EYGAKLLEYTEVKGF+IEN
Sbjct: 121 EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAVKAKEYGAKLLEYTEVKGFLIENN 180
Query: 181 EIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP 240
EIKGVKTN+G IKTGIVVNATNAWA LINAMAGIK KIPIEPYKHQAVITQPIKRGTINP
Sbjct: 181 EIKGVKTNKGIIKTGIVVNATNAWANLINAMAGIKTKIPIEPYKHQAVITQPIKRGTINP 240
Query: 241 MVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLV 300
MVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLL+
Sbjct: 241 MVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLI 300
Query: 301 LRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLP 360
LRTWAGYYAKTPDSNPAIG+IEEL+DYYIAAGFSGHGFMMAPAVGEM+A+LITKGK+KLP
Sbjct: 301 LRTWAGYYAKTPDSNPAIGRIEELNDYYIAAGFSGHGFMMAPAVGEMVAELITKGKTKLP 360
Query: 361 VEWYDPYRFERGELRTAALQMG 382
VEWYDPYRFERGELRTAALQMG
Sbjct: 361 VEWYDPYRFERGELRTAALQMG 382
>pir||B71184 probable sarcosine oxidase - Pyrococcus horikoshii
>gi|3258182|dbj|BAA30865.1| (AP000007) 377aa long
hypothetical sarcosine oxidase [Pyrococcus horikoshii]
Length = 377
Score = 419 bits (1066), Expect = e-116
Identities = 209/379 (55%), Positives = 275/379 (72%), Gaps = 11/379 (2%)
Query: 10 VIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVMKRSVE 69
+IGGGI+G++ A+ELAK G +V V EKR+ GSGSTFRC +GIR QF DEAN+R+MK S++
Sbjct: 4 IIGGGIIGIATAYELAKLGEEVIVFEKRYFGSGSTFRCASGIRAQFTDEANIRLMKYSID 63
Query: 70 LWKKYSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIVP 127
WKK SEE Y F+QTGYLFL EEEV+ FKRNI++QNKFGVPT+LITPEEAKEIVP
Sbjct: 64 KWKKLSEELEYEVMFQQTGYLFLATSEEEVKAFKRNIKLQNKFGVPTKLITPEEAKEIVP 123
Query: 128 LLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEIKGVKT 187
L+ +A +WNP DGKA PF + + G + YTEV G I+ + +KT
Sbjct: 124 PLNADAFLAGAWNPEDGKASPFHTLYGYKRAGEKLGVRFYPYTEVIG--IKKNDKWIIKT 181
Query: 188 NRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPMVISFKY 247
RG + ++VNATNAW + IN+M G K +PI+P+KHQ V T+PI+RG I P+V +
Sbjct: 182 TRGEFRVDVIVNATNAWGRRINSMIG-KDIVPIKPFKHQLVKTEPIERGQIEPLVCPPAW 240
Query: 248 GHAYLTQTFH-GGIIGGIGYEI-GPTYDLTPTYEFLREVSYYFTKIIPALKNLLVLRTWA 305
+Y+ Q GG+I G E G D+TPTY+F++E + KI+PALK++ ++R WA
Sbjct: 241 SDSYVIQDGEDGGVICGTALEYKGNPDDVTPTYDFVKEALKWAVKIVPALKHVHIIRQWA 300
Query: 306 GYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLPV--EW 363
GYYAKTPD+NPAIG++EE D+Y+A GFSGHGFMMAPAV + +A+ I KGK+K+P+ EW
Sbjct: 301 GYYAKTPDNNPAIGELEE--DFYVAIGFSGHGFMMAPAVSQALAEKIVKGKTKVPLDWEW 358
Query: 364 YDPYRFERGELRTAALQMG 382
+DPYRFERGELR++A Q+G
Sbjct: 359 FDPYRFERGELRSSAFQIG 377
>pir||F75144 sarcosine oxidase, chain beta (soxb) PAB0214 - Pyrococcus abyssi
(strain Orsay) >gi|5457747|emb|CAB49237.1| (AJ248284)
sarcosine oxidase, subunit beta (soxB) [Pyrococcus
abyssi]
Length = 379
Score = 414 bits (1052), Expect = e-114
Identities = 209/380 (55%), Positives = 275/380 (72%), Gaps = 11/380 (2%)
Query: 10 VIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVMKRSVE 69
VIGGGI+GV+ A+ELAK G +V V EKR+ GSGSTFRC +GIR QF DEAN+++MK S+E
Sbjct: 4 VIGGGIIGVATAYELAKLGEEVVVFEKRYFGSGSTFRCASGIRAQFTDEANIKLMKYSIE 63
Query: 70 LWKKYSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIVP 127
WK+ SEE +N F+QTGYLFL EEEVE FK+NI++QN+FGVPT+LITPEEAKEIVP
Sbjct: 64 RWKELSEELGHNIMFQQTGYLFLATTEEEVEAFKKNIKLQNRFGVPTKLITPEEAKEIVP 123
Query: 128 LLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEIKGVKT 187
L++ E +A +WNP DGKA PF A+ + G K YTEV +KT
Sbjct: 124 PLNVEEFLAGAWNPEDGKASPFHTLYAYKKAGEKLGVKFYPYTEVVWIDGGREHKWKIKT 183
Query: 188 NRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPMVISFKY 247
+G + ++VNATNAW IN+M G K +PI P+KHQ V T+P++RG I P+V +
Sbjct: 184 TKGEFEVDVIVNATNAWGSRINSMIG-KDIVPITPFKHQLVKTEPLERGQIEPLVCPPAW 242
Query: 248 GHAYLTQTFH-GGIIGG--IGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLVLRTW 304
+Y+ Q GG+I G + Y+ P D+TPTY+F++EV + KIIPAL+++ V+R W
Sbjct: 243 NDSYVIQDGEDGGVICGTALEYKSDPD-DVTPTYDFVKEVLKWAVKIIPALRHVHVVRQW 301
Query: 305 AGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLPV--E 362
AG+YAKTPD+NPAIG++ E D+Y+A GFSGHGFMMAPAV + +A+ I KG++K+P+ E
Sbjct: 302 AGHYAKTPDNNPAIGELVE--DFYVAIGFSGHGFMMAPAVAQALAERIVKGRTKVPLDWE 359
Query: 363 WYDPYRFERGELRTAALQMG 382
WYDPYRFERGELRT+A Q+G
Sbjct: 360 WYDPYRFERGELRTSAFQIG 379
>gi|11497890 sarcosine oxidase, subunit beta (soxB) [Archaeoglobus fulgidus]
>gi|7483942|pir||B69284 sarcosine oxidase, subunit beta
(soxB) homolog - Archaeoglobus fulgidus
>gi|2650362|gb|AAB90958.1| (AE001086) sarcosine oxidase,
subunit beta (soxB) [Archaeoglobus fulgidus]
Length = 354
Score = 182 bits (456), Expect = 8e-45
Identities = 113/366 (30%), Positives = 196/366 (52%), Gaps = 19/366 (5%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVMKR 66
+V VIGGG+ G+S A+ LAK G DV V E++++ G++ R G+ QF +EA +++ KR
Sbjct: 2 KVAVIGGGVAGLSAAYFLAKAGADVKVFEQKYLLYGASGRNSGGLTAQFTNEAMIKLAKR 61
Query: 67 SVELWKKYSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKE 124
++EL+ + E +NF ++ GY+ + EE + + +E Q K GV +++ PE KE
Sbjct: 62 TLELYDELQSEVGFNFLLRRDGYVKIAGKGEEAK-LREEVEFQRKAGVKVKMVEPEFVKE 120
Query: 125 IVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEIKG 184
+ P ++ S AAS+ G P+ A RE G ++ +YT + E+KG
Sbjct: 121 LFPDINTSAFTAASYFADGGVVFPWPVVWGLAKGCRELGVEIYDYTPA------SVEVKG 174
Query: 185 ----VKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP 240
VK + + K ++NA AW+ I+ AG+++ + ++ + +T+ +K ++P
Sbjct: 175 NDLTVKASGESYKVDYIINAAGAWSNEISQQAGVELNNKV--FREEICVTESLK-PYLDP 231
Query: 241 MVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLV 300
++ G Y +Q+ G I+GGI + + +FL + T+++P L+ + V
Sbjct: 232 YIMDISTG-VYFSQSMRGEIVGGILGSETDRLETKASLDFLTTYAKRVTELVPKLRGMAV 290
Query: 301 LRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLP 360
LR WAG Y + P IG+ ++ + GF HG +A A GE +A+LI KG+S +
Sbjct: 291 LRQWAGLYDAGRNGMPVIGQ-TKVRGFIQLNGFGRHGMSLALAAGEGVAELIVKGRSSI- 348
Query: 361 VEWYDP 366
VE + P
Sbjct: 349 VEPFKP 354
>sp|P40875|SAOX_CORS1 SARCOSINE OXIDASE BETA SUBUNIT (SARCOSINE OXIDASE SUBUNIT B)
>gi|2127436|pir||I40887 sarcosine oxidase (EC 1.5.3.1)
beta chain [validated] - Corynebacterium sp
>gi|927589|gb|AAC43459.1| (U23955) sarcosine oxidase
beta subunit [Corynebacterium sp.]
Length = 405
Score = 172 bits (431), Expect = 7e-42
Identities = 114/382 (29%), Positives = 193/382 (49%), Gaps = 15/382 (3%)
Query: 3 PERS-EVVVIGGGIVGVSIAHELAKRG--VDVTVLEKRFIGSGSTFRCGTGIRQQFNDEA 59
P++S +VV++GGG G++ A+ LAK +V VLEK ++ G+ R T IR + +
Sbjct: 18 PKKSYDVVIVGGGGHGLATAYYLAKNHGITNVAVLEKGWLAGGNMARNTTIIRSNYLWDE 77
Query: 60 NVRVMKRSVELWKKYSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
+ + ++S++LW++ EE Y+F F Q G L L + +V R +E GV +
Sbjct: 78 SAGIYEKSLKLWEQLPEELDYDFLFSQRGVLNLAHTLGDVRESVRRVEANKFNGVDAEWL 137
Query: 118 TPEEAKEIVPLLDISE-----VIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEV 172
TPE+ KE+ P+++I + V+ A++ P G A AFA KA E G +++ EV
Sbjct: 138 TPEQVKEVCPIINIGDDIRYPVMGATYQPRAGIAKHDHVAWAFARKANEMGVDIIQNCEV 197
Query: 173 KGFIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
GF+ + ++ GVKT RGTI G V A + ++ +AG ++PI+ + QA++++
Sbjct: 198 TGFLKDGEKVTGVKTTRGTIHAGKVALAGAGHSSVLAELAGF--ELPIQSHPLQALVSEL 255
Query: 233 IKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKII 292
+ ++P V+ + H Y++Q G ++ G G + Y + + E ++
Sbjct: 256 FE--PVHPTVVMSNHIHVYVSQAHKGELVMGAGIDSYNGYGQRGAFHVIEEQMAAAVELF 313
Query: 293 PALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLI 352
P VLRTW G T D++P I K + + Y+ G+ GF P G +A I
Sbjct: 314 PIFARAHVLRTWGGIVDTTMDASPIISK-TPIQNLYVNCGWGTGGFKGTPGAGFTLAHTI 372
Query: 353 TKGKSKLPVEWYDPYRFERGEL 374
++ + RFE G L
Sbjct: 373 ANDEAHALNAPFSLERFETGHL 394
>sp|Q63342|M2GD_RAT DIMETHYLGLYCINE DEHYDROGENASE, MITOCHONDRIAL PRECURSOR (ME2GLYDH)
>gi|111583|pir||S16133 dimethylglycine dehydrogenase (EC
1.5.99.2) - rat >gi|56689|emb|CAA39468.1| (X55995)
dimethylglycine dehydrogenase [Rattus norvegicus]
Length = 857
Score = 152 bits (379), Expect = 9e-36
Identities = 113/389 (29%), Positives = 188/389 (48%), Gaps = 27/389 (6%)
Query: 4 ERSEVVVIGGGIVGVSIAHELAKRGV-DVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVR 62
+R+E V+IGGG VGVS+A+ LAK G+ DV +LEK + +GST+ G+ F+ N++
Sbjct: 41 DRAETVIIGGGCVGVSLAYHLAKAGMRDVVLLEKSELTAGSTWHAA-GLTTYFHPGINLK 99
Query: 63 -VMKRSVELWKKYSEEYN--FSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
+ S++L+++ EE F Q G + L E V+ FK + N +I P
Sbjct: 100 KIHYDSIKLYERLEEETGQVVGFHQPGSIRLATTPERVDEFKYQMTRTNWHATEQYIIEP 159
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIEN 179
E+ E+ PLL++ +++A +NP DG DP+ T A A AR+YG L V
Sbjct: 160 EKIHELFPLLNMDKILAGLYNPGDGHIDPYSLTMALATGARKYGVLLKYPAPVTSLKPRP 219
Query: 180 GEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPI---KRG 236
V+T +G+++ +VNA WA+ + M G+ P+ P +HQ V+T I K
Sbjct: 220 DGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDH--PLIPVQHQYVVTSTIPEVKAL 277
Query: 237 TINPMVISFKYGHAYLTQTFHGGIIGG-------------IGYEIGPTYDLTPTYEFLRE 283
V+ G YL Q G + G + + + P + L
Sbjct: 278 KRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVAHGVPPGFGKELFESDLDR 337
Query: 284 VSYYFT---KIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMM 340
++ + +++P LK ++ G +PD P +G + + +Y++A GF G+G +
Sbjct: 338 ITEHVEAAMEMVPVLKKADIINIVNGPITYSPDILPMVGPHQGVRNYWVAIGF-GYGIIH 396
Query: 341 APAVGEMIADLITKGKSKLPVEWYDPYRF 369
A VG+ ++D I G+ + DP R+
Sbjct: 397 AGGVGKYLSDWILHGEPPFDLIELDPNRY 425
>gi|7019365 dimethylglycine dehydrogenase precursor [Homo sapiens]
>gi|11133608|sp|Q9UI17|M2GD_HUMAN DIMETHYLGLYCINE
DEHYDROGENASE, MITOCHONDRIAL PRECURSOR (ME2GLYDH)
>gi|6650622|gb|AAF21941.1|AF111858_1 (AF111858)
dimethylglycine dehydrogenase precursor [Homo sapiens]
Length = 866
Score = 152 bits (379), Expect = 9e-36
Identities = 118/394 (29%), Positives = 191/394 (47%), Gaps = 37/394 (9%)
Query: 4 ERSEVVVIGGGIVGVSIAHELAKRGV-DVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVR 62
+R+E V+IGGG VGVS+A+ LAK G+ DV +LEK + +GST+ G+ F+ N++
Sbjct: 48 DRAETVIIGGGCVGVSLAYHLAKAGMKDVVLLEKSELTAGSTWHAA-GLTTYFHPGINLK 106
Query: 63 -VMKRSVELWKKYSEEYN--FSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
+ S++L++K EE F Q G + L V+ FK + LI P
Sbjct: 107 KIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTGWHATEQYLIEP 166
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIEN 179
E+ +E+ PLL++++V+A +NP DG DP+ T A A AR+ GA L V +
Sbjct: 167 EKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCGALLKYPAPVTSLKARS 226
Query: 180 GEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPI---KRG 236
V+T +G+++ +VNA WA+ + M G+ + P+ P +HQ V+T I K
Sbjct: 227 DGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGL--EHPLIPVQHQYVVTSTISEVKAL 284
Query: 237 TINPMVISFKYGHAYLTQTFHG---------------------GIIGGIGYEIGPTYDLT 275
V+ G YL Q G G+ G G E+ + DL
Sbjct: 285 KRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFES-DLD 343
Query: 276 PTYEFLREVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSG 335
E ++ +++P LK ++ G +PD P +G + + +Y++A GF G
Sbjct: 344 RIMEHIKAA----MEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGF-G 398
Query: 336 HGFMMAPAVGEMIADLITKGKSKLPVEWYDPYRF 369
+G + A VG+ ++D I G+ + DP R+
Sbjct: 399 YGIIHAGGVGKYLSDWILHGEPPFDLIELDPNRY 432
>gi|11417071 dimethylglycine dehydrogenase precursor [Homo sapiens]
Length = 866
Score = 152 bits (379), Expect = 9e-36
Identities = 118/394 (29%), Positives = 191/394 (47%), Gaps = 37/394 (9%)
Query: 4 ERSEVVVIGGGIVGVSIAHELAKRGV-DVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVR 62
+R+E V+IGGG VGVS+A+ LAK G+ DV +LEK + +GST+ G+ F+ N++
Sbjct: 48 DRAETVIIGGGCVGVSLAYHLAKAGMKDVVLLEKSELTAGSTWHAA-GLTTYFHPGINLK 106
Query: 63 -VMKRSVELWKKYSEEYN--FSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
+ S++L++K EE F Q G + L V+ FK + LI P
Sbjct: 107 KIHYDSIKLYEKLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTGWHATEQYLIEP 166
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIEN 179
E+ +E+ PLL++++V+A +NP DG DP+ T A A AR+ GA L V +
Sbjct: 167 EKIQEMFPLLNMNKVLAGLYNPGDGHIDPYSLTMALAAGARKCGALLKYPAPVTSLKARS 226
Query: 180 GEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPI---KRG 236
V+T +G+++ +VNA WA+ + M G+ + P+ P +HQ V+T I K
Sbjct: 227 DGTWDVETPQGSMRANRIVNAAGFWAREVGKMIGL--EHPLIPVQHQYVVTSTISEVKAL 284
Query: 237 TINPMVISFKYGHAYLTQTFHG---------------------GIIGGIGYEIGPTYDLT 275
V+ G YL Q G G+ G G E+ + DL
Sbjct: 285 KRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKVQDSWVTNGVPPGFGKELFES-DLD 343
Query: 276 PTYEFLREVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSG 335
E ++ +++P LK ++ G +PD P +G + + +Y++A GF G
Sbjct: 344 RIMEHIKAA----MEMVPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVAIGF-G 398
Query: 336 HGFMMAPAVGEMIADLITKGKSKLPVEWYDPYRF 369
+G + A VG+ ++D I G+ + DP R+
Sbjct: 399 YGIIHAGGVGKYLSDWILHGEPPFDLIELDPNRY 432
>gb|AAD53398.2|AF095735_1 (AF095735) sarcosine dehydrogenase [Homo sapiens]
Length = 918
Score = 147 bits (367), Expect = 2e-34
Identities = 108/388 (27%), Positives = 186/388 (47%), Gaps = 25/388 (6%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVL-EKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK G+ VL E+ + SG+T+ + Q +
Sbjct: 63 LPSTANVVVIGGGSLGCQTLYHLAKLGMSGAVLLERERLTSGTTWHTAGLLWQLRPSDVE 122
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + + + EE + + Q G LF+ + + ++ +KR + + +GV + ++
Sbjct: 123 VELLAHTRRVVSRELEEETGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVL 182
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K + PL+++ ++ + P DG DP T A A GA+++E V G +
Sbjct: 183 SPAETKTLYPLMNVDDLYGTLYVPHDGTMDPAGTCTTLARAASARGAQVIENCPVTGIRV 242
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + GV+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 243 WTDDFGVRRVAGVETQHGSIQTPCVVNCAGVWASAVGRMAG--VKVPLVAMHHAYVVTER 300
Query: 233 IKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTY--DLTPTYEF-LREVSY-YF 288
I+ G N + YL G + GYE P + +++ + F L ++ + F
Sbjct: 301 IE-GIQNMPNVRDHDASVYL--RLQGDALSVGGYEANPIFWEEVSDKFAFGLFDLDWEVF 357
Query: 289 TKII-------PALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMA 341
T+ I P L+ + T G + TPD P +G+ EL +++ GF+ G M+
Sbjct: 358 TQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLG 417
Query: 342 PAVGEMIADLITKGKSKLPVEWYDPYRF 369
G+ +A I G+ + + YD RF
Sbjct: 418 GGCGQELAHWIIHGRPEKDMHGYDIRRF 445
>gb|AAD32214.1| (AF140745) sarcosine dehydrogenase [Homo sapiens]
>gi|5453465|gb|AAD43585.1|AF162428_1 (AF162428)
sarcosine dehydrogenase [Homo sapiens]
Length = 918
Score = 147 bits (367), Expect = 2e-34
Identities = 108/388 (27%), Positives = 186/388 (47%), Gaps = 25/388 (6%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVL-EKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK G+ VL E+ + SG+T+ + Q +
Sbjct: 63 LPSTANVVVIGGGSLGCQTLYHLAKLGMSGAVLLERERLTSGTTWHTAGLLWQLRPSDVE 122
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + + + EE + + Q G LF+ + + ++ +KR + + +GV + ++
Sbjct: 123 VELLAHTRRVVSRELEEETGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVL 182
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K + PL+++ ++ + P DG DP T A A GA+++E V G +
Sbjct: 183 SPAETKTLYPLMNVDDLYGTLYVPHDGTMDPAGTCTTLARAASARGAQVIENCPVTGIRV 242
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + GV+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 243 WTDDFGVRRVAGVETQHGSIQTPCVVNCAGVWASAVGRMAG--VKVPLVAMHHAYVVTER 300
Query: 233 IKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTY--DLTPTYEF-LREVSY-YF 288
I+ G N + YL G + GYE P + +++ + F L ++ + F
Sbjct: 301 IE-GIQNMPNVRDHDASVYL--RLQGDALSVGGYEANPIFWEEVSDKFAFGLFDLDWEVF 357
Query: 289 TKII-------PALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMA 341
T+ I P L+ + T G + TPD P +G+ EL +++ GF+ G M+
Sbjct: 358 TQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLG 417
Query: 342 PAVGEMIADLITKGKSKLPVEWYDPYRF 369
G+ +A I G+ + + YD RF
Sbjct: 418 GGCGQELAHWIIHGRPEKDMHGYDIRRF 445
>gb|AAD13137.1| (AF047190) dimethylglycine dehydrogenase-like protein isoform 2;
putative sarcosine dehydrogenase [Homo sapiens]
>gi|4454259|emb|CAA11257.1| (AJ223317) sarcosine
dehydrogenase [Homo sapiens]
Length = 475
Score = 146 bits (366), Expect = 3e-34
Identities = 108/388 (27%), Positives = 186/388 (47%), Gaps = 25/388 (6%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVL-EKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK G+ VL E+ + SG+T+ + Q +
Sbjct: 63 LPSTANVVVIGGGSLGCQTLYHLAKLGMSGAVLLERERLTSGTTWHTAGLLWQLRPSDVE 122
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + + + EE + + Q G LF+ + + ++ +KR + + +GV + ++
Sbjct: 123 VELLAHTRRVVSRELEEETGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVL 182
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K + PL+++ ++ + P DG DP T A A GA+++E V G +
Sbjct: 183 SPAETKTLYPLMNVDDLYGTLYVPHDGTMDPAGTCTTLARAASARGAQVIENCPVTGIRV 242
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + GV+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 243 WTDDFGVRRVAGVETQHGSIQTPCVVNCAGVWASAVGRMAG--VKVPLVAMHHAYVVTER 300
Query: 233 IKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTY--DLTPTYEF-LREVSY-YF 288
I+ G N + YL G + GYE P + +++ + F L ++ + F
Sbjct: 301 IE-GIQNMPNVRDHDASVYL--RLQGDALSVGGYEANPIFWEEVSDKFAFGLFDLDWEVF 357
Query: 289 TKII-------PALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMA 341
T+ I P L+ + T G + TPD P +G+ EL +++ GF+ G M+
Sbjct: 358 TQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSVGMMLG 417
Query: 342 PAVGEMIADLITKGKSKLPVEWYDPYRF 369
G+ +A I G+ + + YD RF
Sbjct: 418 GGCGQELAHWIIHGRPEKDMHGYDIRRF 445
>gb|AAK40533.1| Sarcosine oxidase, subunit beta (soxB-like) [Sulfolobus
solfataricus]
Length = 372
Score = 146 bits (364), Expect = 5e-34
Identities = 110/379 (29%), Positives = 191/379 (50%), Gaps = 27/379 (7%)
Query: 8 VVVIGGGIVGVSIAHELAKRGV---DVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVM 64
++++G G G+S+A+ L K+G D+T++E + IG GS+ R R F + NV
Sbjct: 2 IIIVGAGSHGLSLAYHLVKKGYSKKDITLVEWKRIGYGSSSRNAARYRYHFYSKENVEYA 61
Query: 65 KRSVELWKKYSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEA 122
++ KK S+E YN +TGYL+++ E+E FK+ + + + + I E
Sbjct: 62 INAISYLKKQSKELEYNSLLLRTGYLWVINGEQEYSLFKKLDSFWSSYNIGGKFI---EC 118
Query: 123 KEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEI 182
K+ + + P DG ++ ++ + + Y +V I ++ +I
Sbjct: 119 KDNYAYIKFEGQCYLA--PQDGAFHHDYILYSYYYSIKDKVSTI--YDKVVSLIKDDSKI 174
Query: 183 KGVKTNRGTIKTGIVVNAT-NAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPM 241
KGVK + G G V T AW+ + ++ I +++PI P K + IT+P+ R + P+
Sbjct: 175 KGVKLSSGKELYGDKVIVTAGAWSGEV--LSSIGIEVPIFPEKKEIYITEPL-RYFLEPL 231
Query: 242 VISFKYGHAYLTQTFHGGIIGGIG----YEIGP-TYDLTPTYEFLREVSYYFTKIIPALK 296
+I K Y +QT G IIGGI Y+ T ++ +FL+ + ++I +
Sbjct: 232 IIDSKL-QIYFSQTLKGEIIGGIEDKREYKFEEFTISISNAIKFLKGIR----ELIKGID 286
Query: 297 NLLVLRTWAGYYAKTPDSNPAIGKIEELSD-YYIAAGFSGHGFMMAPAVGEMIADLITKG 355
+ +LR W+GYY TPDS+ +G + + +I AG+SGHG M AP G+++ADLI
Sbjct: 287 GIGILRGWSGYYEMTPDSSQIMGYSNDWPEGLFIDAGYSGHGMMFAPYSGKIMADLIADN 346
Query: 356 KSKLPVEWYDPYRFERGEL 374
++ + P RF+ +L
Sbjct: 347 YKNKFIDIFSPSRFKLNKL 365
>gb|AAD03414.1| (AF067650) sarcosine dehydrogenase [Rattus norvegicus]
Length = 919
Score = 146 bits (364), Expect = 5e-34
Identities = 106/388 (27%), Positives = 187/388 (47%), Gaps = 25/388 (6%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVD-VTVLEKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK GV V +LE+ + SG+T+ + Q +
Sbjct: 64 LPSTANVVVIGGGSLGCQTLYHLAKLGVGGVVLLERERLTSGTTWHTAGLLWQLRPSDVE 123
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + ++ EE + + Q G LF+ +++ ++ +KR + + +G+ + ++
Sbjct: 124 VELLAHTRQVVSHDLEEETGLHTGWIQNGGLFIASNQQRLDEYKRLMSLGKAYGIESHVL 183
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K++ PL+++ ++ + P DG DP T A GA+++E V G +
Sbjct: 184 SPAETKDLYPLMNVDDLYGTLYVPRDGTMDPAGTCTTLTRAAVARGAQVIENCAVTGIRV 243
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + V+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 244 RTDDFGVRRVTAVETQHGSIQTPCVVNCAGVWASSVGRMAG--VKVPLVAMHHAYVVTER 301
Query: 233 IKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTY--DLTPTYEF-LREVSY-YF 288
I+ G N + YL G + GYE P + +++ + F L ++ + F
Sbjct: 302 IE-GIQNMPNVRDHDASVYL--RLQGDALSVGGYEANPIFWDEVSDKFAFGLFDLDWDVF 358
Query: 289 TKII-------PALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMA 341
T+ I P L+ + T G + TPD P +G+ EL +++ GF+ G M+
Sbjct: 359 TQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLG 418
Query: 342 PAVGEMIADLITKGKSKLPVEWYDPYRF 369
G+ +A I G+ + + YD RF
Sbjct: 419 GGCGQELAHWIVHGRPEKDMYSYDIRRF 446
>pir||H82968 sarcosine oxidase beta subunit PA5416 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9951743|gb|AAG08801.1|AE004954_3 (AE004954)
sarcosine oxidase beta subunit [Pseudomonas aeruginosa]
Length = 416
Score = 144 bits (360), Expect = 1e-33
Identities = 105/377 (27%), Positives = 182/377 (47%), Gaps = 14/377 (3%)
Query: 7 EVVVIGGGIVGVSIAHELAKR-GV-DVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVM 64
+VV++GGG G++ A+ LAK G+ +V V+EK ++G G+T R T +R + + + +
Sbjct: 34 DVVIVGGGGHGLATAYYLAKEHGISNVAVVEKGWLGGGNTARNTTIVRSNYLWDESALLY 93
Query: 65 KRSVELWKKYSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEA 122
+ +++LW+ S++ YN F Q G L + +++ +R + GV L+ ++
Sbjct: 94 EHAMKLWEGLSQDLNYNVMFSQRGVYNLCHTLQDMRDGERRVSANRLNGVDGELLNAQQV 153
Query: 123 KEIVPLLDISE-----VIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
E +P LD S+ ++ A+ G A FA A G L++ TEV GF
Sbjct: 154 AEEIPYLDCSKSTRYPIMGATIQRRGGVARHDAVAWGFARAADALGVDLIQQTEVTGFRK 213
Query: 178 ENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGT 237
++G + GV+TNRG I V T + + +AG + +P+E + QA++++P+K
Sbjct: 214 QDGAVIGVETNRGFIGARRVGVVTAGNSGHMARLAGFR--LPLESHPLQALVSEPLK--P 269
Query: 238 INPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKN 297
I VI H Y++Q+ G ++ G G + Y +Y + ++ P L
Sbjct: 270 IIDSVIMSNAVHGYISQSDKGDLVIGAGIDGYNGYGQRGSYPVIEHTLQAIVEMFPVLSR 329
Query: 298 LLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKS 357
+ + R W G TPD+ P IGK + + + G+ GF P G + A + KG+
Sbjct: 330 VRMNRQWGGIVDTTPDACPIIGK-TPVQNLFFNCGWGTGGFKATPGSGNVFAATLAKGEP 388
Query: 358 KLPVEWYDPYRFERGEL 374
+ RF G L
Sbjct: 389 HPLAAPFSIERFHSGAL 405
>gb|AAF57796.1| (AE003802) CG6385 gene product [Drosophila melanogaster]
Length = 894
Score = 140 bits (350), Expect = 2e-32
Identities = 104/385 (27%), Positives = 179/385 (46%), Gaps = 22/385 (5%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANV 61
LP ++VVVIGGG G + LA+RGV +LE+ + +G+T+ + + ++ ++
Sbjct: 33 LPGAADVVVIGGGSAGCHTLYHLARRGVKAVLLERAQLTAGTTWHTAGLLWRLRPNDVDI 92
Query: 62 RVMKRSVELWKKYSEEYNFS--FKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
+++ S + ++ EE + Q G +F+ ++E ++ ++R + + G+ ++++P
Sbjct: 93 QLLANSRRMLQQLEEETELDPGWIQNGGIFIAHNETRLDEYRRLATVGSALGIENQVLSP 152
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIE- 178
E+ +++ PLLD S + A ++P DG DP A A GA+++E V ++E
Sbjct: 153 EDTQKLFPLLDPSAFVGALYSPGDGVMDPAMLCAALKKAATNLGAQVIENCGVDDLLLEQ 212
Query: 179 ---NGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKR 235
++ GV T G IK VVNAT W + + A G +P+ P KH ++++ I
Sbjct: 213 TARGKKVVGVSTPFGDIKAEKVVNATGVWGRDLVARHG--THLPLVPMKHAYIVSESIPG 270
Query: 236 GTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEF---LREVSYYF---- 288
P + Y + Q G I GYE P +F L E+ +
Sbjct: 271 VRGLPNIRDHDYSTYFRIQ---GDAICMGGYEPNPILLEPVPKDFHFGLYELDWSVFETH 327
Query: 289 ----TKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAV 344
K+ P+ V T G + TPD P +G L Y GF+ G M
Sbjct: 328 VEGAQKLCPSYAKYGVKSTVCGPESFTPDHKPLMGPDPNLDGLYHNCGFNSAGMMFGGGC 387
Query: 345 GEMIADLITKGKSKLPVEWYDPYRF 369
GE A + +G+ LP+ +D RF
Sbjct: 388 GEQTALWVIQGQPDLPMFGFDLRRF 412
>sp|O87388|SAOX_RHIME SARCOSINE OXIDASE BETA SUBUNIT (SARCOSINE OXIDASE SUBUNIT B)
>gi|3687680|gb|AAC62218.1| (AF055582) sarcosine oxidase
subunit B [Sinorhizobium meliloti]
Length = 416
Score = 139 bits (348), Expect = 4e-32
Identities = 106/376 (28%), Positives = 180/376 (47%), Gaps = 13/376 (3%)
Query: 7 EVVVIGGGIVGVSIAHELAKR-GV-DVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVM 64
+V+++GGG G++ A+ LAK G+ +V VLEK +IGSG+ R T IR + N
Sbjct: 33 DVIIVGGGGHGLATAYYLAKEFGITNVAVLEKNYIGSGNVGRNTTIIRSNYLLPGNNPFY 92
Query: 65 KRSVELWKKYSEEYNFS--FKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEA 122
+ S++LW+ +++NF+ Q G L L + + + + + R GV L+
Sbjct: 93 ELSMKLWEGLEQDFNFNAMVSQRGVLNLFHSDAQRDAYTRRGNAMRLHGVDAELLYRAAV 152
Query: 123 KEIVPLLDISE----VIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIE 178
++++P LD + G +A A G +++ EV G E
Sbjct: 153 RKMLPFLDFDNARFPIQGGLLQRRGGTVRHDAVAWGYARGADSRGVDIIQNCEVTGIRRE 212
Query: 179 NGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTI 238
NG + GV+T+RG I + A + + MAG+ ++PIE + QA +++ +K
Sbjct: 213 NGRVIGVETSRGFIGCAKLALAAAGNSSQVAEMAGL--RLPIESHVLQAFVSEGLK--PF 268
Query: 239 NPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNL 298
V++F GH Y++Q+ GG++ G + +Y + V+ +IPAL +
Sbjct: 269 IDGVVTFGAGHFYVSQSDKGGLVFGGDIDGYNSYAQRGNLATVEHVAEAGKAMIPALSRV 328
Query: 299 LVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSK 358
VLR+W G + D +P I + + + Y+ AG+ GF PA G A L+ +G +
Sbjct: 329 RVLRSWGGIMDMSMDGSPIIDR-TPIDNLYLNAGWCYGGFKATPASGFCFAHLLARGAPQ 387
Query: 359 LPVEWYDPYRFERGEL 374
+ RFERG L
Sbjct: 388 KTAAAFRLDRFERGFL 403
>dbj|BAB05760.1| (AP001514) unknown conserved protein [Bacillus halodurans]
Length = 376
Score = 133 bits (331), Expect = 4e-30
Identities = 105/364 (28%), Positives = 183/364 (49%), Gaps = 28/364 (7%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIG-------SGSTFRCGTGIRQQ 54
+ ER ++ +IGGG++G+SIA AK+G +V V+EK +G +G G+ +
Sbjct: 1 MKERFDLCIIGGGVIGLSIAIHTAKKGANVLVVEKDRLGQEASGGRAGLLTTVAEGVDRH 60
Query: 55 -----FNDEANVRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNK 109
F+ E+ +++ EL +K + + + L L E+E + + +Q +
Sbjct: 61 DPYSIFSRESLQYLLQWIPELEEK--TQISSGLVKQNLLRLALHEQEAALLEDHWLMQTE 118
Query: 110 FGVPTRLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEY 169
+ ++ EEA+ + PLL +++I A +P + +P A A AR+ G + E
Sbjct: 119 TNSQIKRLSAEEARTLEPLLS-NDLIMAICSPDEYHLEPSLYLNALARMARQLGVTIREQ 177
Query: 170 TEVKGFIIENGEIKGVKTNRGT-IKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAV 228
TEV FI +N + GV TN GT I + A AW+ L++ +A KVK+P+ P K Q +
Sbjct: 178 TEVIQFIHQNNRMYGVVTNTGTQIVAETTILAAGAWSHLLSRLA--KVKVPVYPLKGQLI 235
Query: 229 ITQPIKRGT--INPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSY 286
+ +K+G +N M+ + K Y+ +G ++ G E ++ T + ++E+
Sbjct: 236 V---LKQGDTDLNVMINTSK---GYILSKPNGTVVLGATSE-KEKFNKFITAKGIQELLP 288
Query: 287 YFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGE 346
F +P LKN + WAG TPD P IG + E +Y+A G S HG +++ G
Sbjct: 289 LFD-YVPELKNWEIQHLWAGLRPATPDGQPIIGYVPEWEGWYLATGHSRHGVLLSGWTGH 347
Query: 347 MIAD 350
++A+
Sbjct: 348 LVAE 351
>gb|AAD13136.1| (AF047004) dimethylglycine dehydrogenase-like protein isoform 1;
putative sarcosine dehydrogenase [Homo sapiens]
Length = 431
Score = 132 bits (329), Expect = 6e-30
Identities = 99/356 (27%), Positives = 171/356 (47%), Gaps = 25/356 (7%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVL-EKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK G+ VL E+ + SG+T+ + Q +
Sbjct: 63 LPSTANVVVIGGGSLGCQTLYHLAKLGMSGAVLLERERLTSGTTWHTAGLLWQLRPSDVE 122
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + + + EE + + Q G LF+ + + ++ +KR + + +GV + ++
Sbjct: 123 VELLAHTRRVVSRELEEETGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVL 182
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K + PL+++ ++ + P DG DP T A A GA+++E V G +
Sbjct: 183 SPAETKTLYPLMNVDDLYGTLYVPHDGTMDPAGTCTTLARAASARGAQVIENCPVTGIRV 242
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + GV+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 243 WTDDFGVRRVAGVETQHGSIQTPCVVNCAGVWASAVGRMAG--VKVPLVAMHHAYVVTER 300
Query: 233 IKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTY--DLTPTYEF-LREVSY-YF 288
I+ G N + YL G + GYE P + +++ + F L ++ + F
Sbjct: 301 IE-GIQNMPNVRDHDASVYL--RLQGDALSVGGYEANPIFWEEVSDKFAFGLFDLDWEVF 357
Query: 289 TKII-------PALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHG 337
T+ I P L+ + T G + TPD P +G+ EL +++ GF+ G
Sbjct: 358 TQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAG 413
>gb|AAC48785.1| (AF026954) pyruvate dehydrogenase phosphatase regulatory subunit
precursor; PDPr [Bos taurus]
Length = 878
Score = 131 bits (325), Expect = 2e-29
Identities = 94/385 (24%), Positives = 181/385 (46%), Gaps = 20/385 (5%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRG-VDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP +++VVV GGGI+G S+A+ L+K G DV +LE+ + +GST C + +
Sbjct: 38 LPAQAQVVVCGGGIMGTSVAYHLSKMGWKDVVLLEQGRLAAGSTRFCAGILSTARHLAIE 97
Query: 61 VRVMKRSVELWKKYSEEYNFS--FKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLIT 118
++ S +L+ + +E + +TG +FL ++ + + KR N G+P +I+
Sbjct: 98 QKMADYSNKLYHQLEQETGIQTGYTRTGSIFLAQTQDRLISLKRINSRLNVIGIPCEIIS 157
Query: 119 PEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIE 178
P++ E+ PLL++ +++ A P D + A A A + G ++ + T + +++
Sbjct: 158 PKKVAELHPLLNVHDLVGAMHVPEDAVVSSADVALALASAASQSGVQIYDRTSILHVMVK 217
Query: 179 NGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKR--G 236
G++ GV+T++G I+ VN WA + + V IP+ +H ++T+P +
Sbjct: 218 KGQVAGVETDKGQIQCQYFVNCAGQWAYELGLCSEEPVSIPLHACEHFYLLTRPWETPLP 277
Query: 237 TINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGP------------TYDLTPTYEFLREV 284
+ P V+ G Y+ + + GGI+ G G+E P +L ++ +
Sbjct: 278 SSTPTVVDAD-GRIYI-RNWQGGILSG-GFEKNPKPIFTEGKNQLEIQNLQEDWDHFEPL 334
Query: 285 SYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAV 344
+ +P L+ L +++ TPD +G+ + Y++ G + G
Sbjct: 335 LSSLLRRMPQLETLEIVKLVNCPETFTPDMRCIMGESPSVRGYFVLVGMNSAGLSFGGGA 394
Query: 345 GEMIADLITKGKSKLPVEWYDPYRF 369
G+ +A+ + G V D RF
Sbjct: 395 GKYLAEWMVYGYPSENVWELDLKRF 419
>gi|8923708 hypothetical protein [Homo sapiens] >gi|11437505|ref|XP_006258.1|
hypothetical protein [Homo sapiens]
>gi|6048970|gb|AAF02421.1|AF103801_1 (AF103801) unknown
[Homo sapiens]
Length = 486
Score = 125 bits (312), Expect = 6e-28
Identities = 108/402 (26%), Positives = 181/402 (44%), Gaps = 50/402 (12%)
Query: 3 PERSEVVVIGGGIVGVSIAHELAK----RG-VDVTVLEKRFIGS-GSTFRCGTGIRQQFN 56
PE S+VV++GGG++G+S+A+ L K RG + V V+E+ S ST GI QQF+
Sbjct: 61 PEHSDVVIVGGGVLGLSVAYWLKKLESRRGAIRVLVVERDHTYSQASTGLSVGGICQQFS 120
Query: 57 DEANVRVMKRSVELWKKYSE--------EYNFSFKQTGYLFLLYDEEEVETFKRNIEIQN 108
N+++ S + +E + F +GYL LL E++ + N+++Q
Sbjct: 121 LPENIQLSLFSASFLRNINEYLAVVDAPPLDLRFNPSGYL-LLASEKDAAAMESNVKVQR 179
Query: 109 KFGVPTRLITPEEAKEIVPLLDISEVIAASWNPTD-GKADPFEATTAFAIKAREYGAKLL 167
+ G L++P++ + P ++ V AS+ D G DP+ K + G
Sbjct: 180 QEGAKVSLMSPDQLRNKFPWINTEGVALASYGMEDEGWFDPWCLLQGLRRKVQSLGVLFC 239
Query: 168 E--------------YTEVKGFIIENGEIKGVKTNRGT----IKTGIVVNATNAWAKLIN 209
+ T+ K +++ VK +R ++ IV+NA AW+ I
Sbjct: 240 QGEVTRFVSSSQRMLTTDDKAVVLKRIHEVHVKMDRSLEYQPVECAIVINAAGAWSAQIA 299
Query: 210 AMAGIK---------VKIPIEPYKHQAVITQ-PIKRGTINPMVISFKYGHAYLTQTFHGG 259
A+AG+ K+P+EP K + P G P+V G + +
Sbjct: 300 ALAGVGEGPPGTLQGTKLPVEPRKRYVYVWHCPQGPGLETPLVADTS-GAYFRREGLGSN 358
Query: 260 IIGGIG---YEIGPTYDLTPTYEFLREVSY-YFTKIIPALKNLLVLRTWAGYYA-KTPDS 314
+GG E +L ++F ++ + + +PA + L V WAGYY T D
Sbjct: 359 YLGGRSPTEQEEPDPANLEVDHDFFQDKVWPHLALRVPAFETLKVQSAWAGYYDYNTFDQ 418
Query: 315 NPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGK 356
N +G + + Y A GFSGHG AP +G +A+++ KG+
Sbjct: 419 NGVVGPHPLVVNMYFATGFSGHGLQQAPGIGRAVAEMVLKGR 460
>pir||E72254 conserved hypothetical protein - Thermotoga maritima (strain MSB8)
>gi|4981997|gb|AAD36502.1|AE001795_5 (AE001795)
conserved hypothetical protein [Thermotoga maritima]
Length = 479
Score = 124 bits (308), Expect = 2e-27
Identities = 102/383 (26%), Positives = 182/383 (46%), Gaps = 27/383 (7%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTVLEKRF-IGSGSTFRCGTGIRQQFND---EANVR 62
+ VVIGGG+ G IA EL K ++VT++EK +G G T + ++D +
Sbjct: 2 KAVVIGGGVFGSLIARELTKYDLEVTLIEKNLDVGWGVTKANSAVVHAGYDDPPESVRAQ 61
Query: 63 VMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVP-TRLITPEE 121
+++ S+E +F FK+ G + +++EE++ +R ++ + GVP ++ +E
Sbjct: 62 FCASGNAMYEDLSKELDFDFKRIGSFVVAFNDEELKELERLLKQGEENGVPGLTILERDE 121
Query: 122 AKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGE 181
+ P L+ EV A + PT G +P+ A A + G KL+ V GF NG
Sbjct: 122 VLSMEPNLN-PEVKYALYAPTAGITEPWMVAIAAVENAVQNGLKLVLGESVVGFEKVNGR 180
Query: 182 IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVI----TQPIKRGT 237
++ V T+RG + IV+N A I +AG + +P+ P K + ++ Q + +
Sbjct: 181 VRKVHTSRGEYEADIVINCAGLHADEIAKLAGAEY-VPLHPRKGEYILLDKKLQGLVKRV 239
Query: 238 INPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTP---------TYEFLREVSYYF 288
I P G L T GGI+ +GPT + P T E L +V +
Sbjct: 240 IFPTPTKISKGILVL-PTVDGGIL------LGPTAEDLPEEMKDRPITTREGLEKVREFT 292
Query: 289 TKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMI 348
+++P+L LV++T++G ++P + I E + ++ G APAV + +
Sbjct: 293 RRLVPSLDFSLVVKTFSGLRPESPQKDFFIKVSETVKNFVNVMATRSPGLTAAPAVAKYV 352
Query: 349 ADLITKGKSKLPVEWYDPYRFER 371
+ + + K ++P+E ++ ER
Sbjct: 353 VEELIQEKMRIPLEKKKDFKPER 375
>gb|AAG18868.1| (AE004990) sarcosine oxidase; SoxB [Halobacterium sp. NRC-1]
Length = 373
Score = 119 bits (295), Expect = 6e-26
Identities = 106/381 (27%), Positives = 168/381 (43%), Gaps = 45/381 (11%)
Query: 8 VVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVMKRS 67
V VIG G VG++ AH+LA+RGVDVTV E+ + + ST R F D + RV R+
Sbjct: 12 VAVIGAGAVGLTAAHDLAERGVDVTVYERDTVAAASTGRAAGICYDAFADAVDARVAARA 71
Query: 68 VELWKKYSEEYNFSFKQTGYL-FLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIV 126
+E +++ S F+ +T Y+ F + + + + G + V
Sbjct: 72 IERFRELSGHGAFTVAETPYVWFATEPGATADAIREQVAAMQRHGRAVEHADADALAAAV 131
Query: 127 PLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEIKGVK 186
P L ++++AA+ A A + Y + + +G I E V
Sbjct: 132 PALHTTDIVAAA-----------IARDAAVVDTTAYARLIADRARDRGVEIRTHEPASVA 180
Query: 187 TN----RGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPMV 242
T+ GT +VV A A +A V +P++PY+ QA +T + P V
Sbjct: 181 TDPVRVNGTAYDDVVV---AAGAHTARVLADAGVPVPLKPYRVQA-LTADAPAAEL-PTV 235
Query: 243 ISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLL--- 299
G Y + G++ G G E + P ++ R+ +F + A+ + L
Sbjct: 236 YDATAG--YYVRPHPEGVLAGDGTE---PVEADPD-DYGRDGDDWF---VDAMDDRLRDR 286
Query: 300 -------VLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLI 352
V R+WAG TPD +P +G++ + Y+AAG+ GHGFM APA G +AD +
Sbjct: 287 FPGYEPAVERSWAGLCTATPDRDPLLGELRD--GLYVAAGWQGHGFMRAPATGAALADDV 344
Query: 353 TKGKSKLPVEWYDPYRFERGE 373
G V +DP RF+ E
Sbjct: 345 LGGDG---VPAFDPTRFDGTE 362
>gb|AAD53399.2|AF095736_1 (AF095736) unknown [Homo sapiens]
Length = 328
Score = 116 bits (287), Expect = 5e-25
Identities = 80/280 (28%), Positives = 135/280 (47%), Gaps = 14/280 (5%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVL-EKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK G+ VL E+ + SG+T+ + Q +
Sbjct: 19 LPSTANVVVIGGGSLGCQTLYHLAKLGMSGAVLLERERLTSGTTWHTAGLLWQLRPSDVE 78
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + + + EE + + Q G LF+ + + ++ +KR + + +GV + ++
Sbjct: 79 VELLAHTRRVVSRELEEETGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVL 138
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K + PL+++ ++ + P DG DP T A A GA+++E V G +
Sbjct: 139 SPAETKTLYPLMNVDDLYGTLYVPHDGTMDPAGTCTTLARAASARGAQVIENCPVTGIRV 198
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + GV+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 199 WTDDFGVRRVAGVETQHGSIQTPCVVNCAGVWASAVGRMAG--VKVPLVAMHHAYVVTER 256
Query: 233 IKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTY 272
I+ G N + YL G + GYE P +
Sbjct: 257 IE-GIQNMPNVRDHDASVYL--RLQGDALSVGGYEANPIF 293
>gi|10955084 agaE [Agrobacterium tumefaciens] >gi|11352852|pir||T45533 agaE
protein [imported] - Agrobacterium tumefaciens plasmid
pTi15955 >gi|2665709|gb|AAB88469.1| (AF242881) agaE
[Agrobacterium tumefaciens]
Length = 437
Score = 115 bits (284), Expect = 1e-24
Identities = 74/216 (34%), Positives = 111/216 (51%), Gaps = 3/216 (1%)
Query: 1 MLPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN 60
+LPE+ +VVVIGGGIVGVS A ELA+RG+ V + EK I + R +RQ D +
Sbjct: 14 ILPEKVDVVVIGGGIVGVSTALELAERGISVALCEKGLIAGEQSGRNWGWVRQMGRDASE 73
Query: 61 VRVMKRSVELWKKYSEEY--NFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLIT 118
+ + S+ LWK + F+QTG +L + + ++ + K G+ +RL+
Sbjct: 74 IPLAVESLNLWKGLNARIGEETGFRQTGIAYLCRNTRQEAEYEAWLVHARKHGLDSRLLK 133
Query: 119 PEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIE 178
EE ++ +P + AA TDG+A+P +A A A A GA ++ V+
Sbjct: 134 KEELRQHLPGM-ADGFTAALHTSTDGRAEPSKAAPAIARGAIRAGAHIITGCAVRSIERS 192
Query: 179 NGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGI 214
G + GV T RG I VV A AW++L + GI
Sbjct: 193 GGAVSGVVTERGRIACSSVVLAGGAWSRLFSGNMGI 228
Score = 72.6 bits (175), Expect = 7e-12
Identities = 38/106 (35%), Positives = 58/106 (53%), Gaps = 3/106 (2%)
Query: 273 DLTPTYEFLREVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAG 332
D P F RE + K PA + + ++WAG TPD+ P IG + ++IA+G
Sbjct: 335 DPKPFERFNREGLAHLVKAFPAFADSRITQSWAGLIDVTPDAIPVIGPAGGIPGFFIASG 394
Query: 333 FSGHGFMMAPAVGEMIADLITKGKSKLPVEWYDPYRFERGELRTAA 378
FSGHGF + P G+++ADL+T + + P+RF+R + AA
Sbjct: 395 FSGHGFGIGPGSGKLMADLVTNAQPSVDPA---PFRFDRFKKTKAA 437
>gi|6005868 sarcosine dehydrogenase; dimethylglycine dehydrogenase-like 1 [Homo
sapiens] >gi|11429603|ref|XP_005639.1| sarcosine
dehydrogenase [Homo sapiens] >gi|5902972|gb|AAD53400.2|
(AF095737) unknown [Homo sapiens]
Length = 396
Score = 114 bits (282), Expect = 2e-24
Identities = 71/242 (29%), Positives = 123/242 (50%), Gaps = 11/242 (4%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVL-EKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK G+ VL E+ + SG+T+ + Q +
Sbjct: 63 LPSTANVVVIGGGSLGCQTLYHLAKLGMSGAVLLERERLTSGTTWHTAGLLWQLRPSDVE 122
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + + + EE + + Q G LF+ + + ++ +KR + + +GV + ++
Sbjct: 123 VELLAHTRRVVSRELEEETGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVL 182
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K + PL+++ ++ + P DG DP T A A GA+++E V G +
Sbjct: 183 SPAETKTLYPLMNVDDLYGTLYVPHDGTMDPAGTCTTLARAASARGAQVIENCPVTGIRV 242
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + GV+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 243 WTDDFGVRRVAGVETQHGSIQTPCVVNCAGVWASAVGRMAG--VKVPLVAMHHAYVVTER 300
Query: 233 IK 234
I+
Sbjct: 301 IE 302
>gb|AAF57379.1| (AE003789) CG3270 gene product [Drosophila melanogaster]
Length = 440
Score = 114 bits (282), Expect = 2e-24
Identities = 106/394 (26%), Positives = 172/394 (42%), Gaps = 44/394 (11%)
Query: 1 MLPERSEVVVIGGGIVGVSIAHELAKRGVDV-----TVLEKRFIG--SGSTFRCGTGIRQ 53
+LP V++IGGG +G S A L R + + ++ +R G S ST G+RQ
Sbjct: 27 VLPGSCGVLIIGGGGMGASSAFWLKSRALQLGRKLNVLVVERDAGYTSASTVLSVGGVRQ 86
Query: 54 QFNDEANVRVMK---RSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKF 110
QF+ N+ + V +++ + + ++ GYL +L E+ +N ++QN+
Sbjct: 87 QFSLAENIEMSLFGYNFVVNGREHLGDVDLCYQPNGYL-ILASEKGAHILAKNSKLQNEL 145
Query: 111 GVPTRLITPEEAKEIVPLLDISEV-IAASWNPTDGKADPFEATTAFAIKAREYGAKLLEY 169
G L+ PE ++ P L V + +G DP+ + KAR GA
Sbjct: 146 GARNELLGPEALRQRFPWLSTEGVELGCHGIDKEGWFDPWALLMGYKKKARALGANFANG 205
Query: 170 TEVKGFIIENGEIKGVKTNRG-----TIKTGIVVNATNAWAKLINAMAGI---------- 214
+ V ++G + G + G T+K V A A++ + +AGI
Sbjct: 206 SVVGFEWNDSGGLSGAVVDAGDVLQRTVKFDTCVLAAGAYSGQVARLAGIGDKEAKEASL 265
Query: 215 KVKIPIEPYKHQAVITQPIKR---GTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPT 271
V +P+EP K + + G P+ + T G+ G P
Sbjct: 266 SVALPVEPRKRYVYVVSTQGKNCPGLATPLTVDPDG-----TYFRRDGLCGNFLCGRSPN 320
Query: 272 YDLTPTYEFLR-EVSYYFTKI-------IPALKNLLVLRTWAGYYA-KTPDSNPAIGKIE 322
D P E L + Y+ T + +PA +++ + +WAG+Y T D+N IG+
Sbjct: 321 EDEEPECETLDVDHGYFETDVWPTLANRVPAFESVKIQSSWAGFYDHNTFDANGVIGRHP 380
Query: 323 ELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGK 356
S+ +IAAGFSGHG PAVG I++LI GK
Sbjct: 381 HYSNLFIAAGFSGHGIQQTPAVGRAISELILDGK 414
>dbj|BAB05153.1| (AP001512) glycine oxidase (sarcosine oxidase) [Bacillus
halodurans]
Length = 365
Score = 112 bits (277), Expect = 8e-24
Identities = 86/376 (22%), Positives = 174/376 (45%), Gaps = 26/376 (6%)
Query: 8 VVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN-----VR 62
++V+GGG++G+++A EL RG VTVLE+ G G G+ F++
Sbjct: 1 MIVLGGGVIGLAVAFELKVRGHQVTVLEQGLCG-GQASGAAAGMLAPFSEIGEDPNDFFH 59
Query: 63 VMKRSVEL---WKKYSEEYN---FSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRL 116
+ S+++ W++ +E + F + Q+G L +Y E + + Q FGV + +
Sbjct: 60 LAHESLKMYPDWQQAVKEISGKSFEYVQSGSLHCVYHEADFLELESRQHWQGTFGVESEI 119
Query: 117 ITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLE---YTEVK 173
++ ++ + + P + E+I A + P + + A I R+ G + + + ++
Sbjct: 120 LSQQQIRALEPNIS-DEIIGAIYYPEESHLFAPDYVKALEIGCRKLGVTIEDEVGHISIE 178
Query: 174 GFIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPI 233
++ E+ ++G + IV AWA +++P+ P + Q + +
Sbjct: 179 AWM-EHQVLRGPSGKQWAGDRLIV--CAGAWAS--EHEETFSLRVPVYPIRGQ-ICAYEV 232
Query: 234 KRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIP 293
G + +V + Y+ +G ++ G +I YD T T + ++ ++ + I P
Sbjct: 233 PAGDVRHIVYT---SQGYIVPKGNGTVVNGASEDIAG-YDTTVTEQGIKRLTNWNGHIFP 288
Query: 294 ALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLIT 353
L L TWAG T D P IG+ ++ A G +G +++P +++AD++
Sbjct: 289 FLNELTPFHTWAGLRPATQDGYPMIGETKKAPHVVFATGHYRNGILLSPITAKVVADVVE 348
Query: 354 KGKSKLPVEWYDPYRF 369
K+++P+ +DP RF
Sbjct: 349 GKKTEVPLTMFDPMRF 364
>gb|AAB03674.1| (U62481) dimethylglycine dehydrogenase-like protein [Rattus
norvegicus] >gi|1449112|gb|AAB04667.1| (L79910)
dimethylglycine dehydrogenase [Rattus norvegicus]
Length = 278
Score = 108 bits (267), Expect = 1e-22
Identities = 67/242 (27%), Positives = 121/242 (49%), Gaps = 11/242 (4%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVD-VTVLEKRFIGSGSTFRCGTGIRQQFNDEAN 60
LP + VVVIGGG +G + LAK GV V +R + SG+T+ + Q +
Sbjct: 24 LPSTANVVVIGGGSLGCQTLYHLAKLGVSGVGAAGERAMTSGTTWHTAGLLWQLRPSDVE 83
Query: 61 VRVMKRSVELWKKYSEE---YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLI 117
V ++ + ++ EE + + Q G F+ +++ ++ +KR + + +G+ + ++
Sbjct: 84 VELLAHTRQVVSHDLEEETGLHTGWIQNGGFFIASNQQRLDEYKRLMSLGKAYGIESHVL 143
Query: 118 TPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFII 177
+P E K++ PL+++ ++ + P DG DP T A GA+++E V G +
Sbjct: 144 SPAETKDLYPLMNVDDLYGTLYVPRDGTMDPAGTCTTLTRAAVARGAQVIENCAVTGIRV 203
Query: 178 ENGE-----IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP 232
+ + V+T G+I+T VVN WA + MAG VK+P+ H V+T+
Sbjct: 204 RTDDFGVRRVTAVETQHGSIQTPCVVNCAGVWASSVGRMAG--VKVPLVAMHHAYVVTER 261
Query: 233 IK 234
I+
Sbjct: 262 IE 263
>pir||B75218 glycerol-3-phosphate dehydrogenase (glpa) PAB0183 - Pyrococcus
abyssi (strain Orsay) >gi|5457702|emb|CAB49193.1|
(AJ248283) glycerol-3-phosphate dehydrogenase (glpA)
[Pyrococcus abyssi]
Length = 497
Score = 108 bits (266), Expect = 2e-22
Identities = 103/380 (27%), Positives = 172/380 (45%), Gaps = 18/380 (4%)
Query: 5 RSEVVVIGGGIVGVSIAHELAK-RGVDVTVLEKR-FIGSGSTFRCGTGIRQQFNDEANVR 62
R++V +IG GI G SIA L+K ++V ++EK +G G + I ++D+
Sbjct: 4 RTKVAIIGAGITGASIARVLSKYENLEVHLIEKNPDVGWGVSKANTAIIHGGYDDDPEKY 63
Query: 63 VMK-----RSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPT-RL 116
M+ + +W ++ ++ G L + +EE+ + ++ +E K GVP R+
Sbjct: 64 PMRARFCVKGNRIWHEWVKQLEIPHVWNGALVVALEEEDFDELEKLLERGIKNGVPEMRI 123
Query: 117 ITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFI 176
+ EE ++ P L+ + A W P G+ P A A A G K T+VKG
Sbjct: 124 VDKEELFQLEPGLN-RNALGALWVPIVGQIAPIPAVIALVENAVANGVKTHLETKVKGIK 182
Query: 177 IENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQ---PI 233
++ GE++G++TN G I+ I++NA +A I+ M G+ I P K + I P
Sbjct: 183 VKRGEVRGLETNDGFIEADIIINAAGLYADEISRMVGLDY-FEIRPRKGEYWIFDEGIPG 241
Query: 234 KRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGP--TYDLTPTYEFLREVSYYFTKI 291
+ + P G T+ +IG ++ P + T E L EV K+
Sbjct: 242 PKRVLFPTPTPISKGIVVTTEISGHLMIGPNAKDLSPEEKENTATTREGLDEVWEGAKKL 301
Query: 292 IPAL-KNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIAD 350
P L V+RT+AG + + I EE+ + AG G APA+ +A+
Sbjct: 302 WPNLPPRSKVIRTFAGLRPEPTGGDFIIRAEEEVWGFINVAGIRSPGLTSAPAIAYYVAE 361
Query: 351 LITKGKSKLPVEW--YDPYR 368
LI + +E ++PYR
Sbjct: 362 LIERELDVKLIEKKNWNPYR 381
>gb|AAF45943.1| (AE003430) CG3626 gene product [Drosophila melanogaster]
Length = 777
Score = 108 bits (266), Expect = 2e-22
Identities = 67/273 (24%), Positives = 126/273 (45%), Gaps = 14/273 (5%)
Query: 110 FGVPTRLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEY 169
+G+ +++PE+ + LL + + W P DG DP A+ I+A+ G +++E+
Sbjct: 14 WGMHCEILSPEQCAQHCELLSLDGIEGGLWIPEDGVCDPQLVCQAYMIEAQRLGVRIVEH 73
Query: 170 TEVKGFIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVI 229
+K E+G+++ V+T G ++ VN T WA+ + ++ VK+P++ +H +
Sbjct: 74 CAIKKIHSEHGKVRSVETTAGDVECEYFVNCTGFWAREVGTLSKPVVKVPLKAVEHHYLH 133
Query: 230 TQPIK-RGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYD------------LTP 276
T+PI+ P V F G + + + GG E Y+ P
Sbjct: 134 TKPIEGLSPDTPFVRDFD-GRIFFRECEGHILAGGFEREAKMVYEDGVIPLSQTARQHPP 192
Query: 277 TYEFLREVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGH 336
++ E+ +P+ ++ + R +PD +G+ E+ +YY+AAG
Sbjct: 193 DWDHFHELLDALLLRVPSFRDATLDRLTNSLQVFSPDCKWILGEAPEIQNYYVAAGNKTM 252
Query: 337 GFMMAPAVGEMIADLITKGKSKLPVEWYDPYRF 369
G + +G ++ DLITKG + L + D RF
Sbjct: 253 GVSASGGIGRVLTDLITKGSTYLDLHILDISRF 285
>sp|O31616|GLOX_BACSU GLYCINE OXIDASE >gi|7475812|pir||B69845 sarcosine oxidase homolog
yjbR - Bacillus subtilis >gi|2633521|emb|CAB13024.1|
(Z99110) similar to sarcosine oxidase [Bacillus
subtilis]
Length = 369
Score = 106 bits (261), Expect = 6e-22
Identities = 104/382 (27%), Positives = 166/382 (43%), Gaps = 37/382 (9%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGI---------RQQFND 57
E VVIGGGI+G +IA+ LAK + + E +G G T G+ R F D
Sbjct: 6 EAVVIGGGIIGSAIAYYLAKENKNTALFESGTMG-GRTTSAAAGMLGAHAECEERDAFFD 64
Query: 58 EANVRVMKRSVELWKKYSEE-YNFSF----KQTGYLF-LLYDEEEVETFKRNIEIQNKFG 111
A S L+K EE Y S + G +F L + EE+V ++ ++ +
Sbjct: 65 FA-----MHSQRLYKGLGEELYALSGVDIRQHNGGMFKLAFSEEDVLQLRQMDDLDS--- 116
Query: 112 VPTRLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTE 171
+ EE E P ++ AS+ D +P+ A+ A+ GA++ E+T
Sbjct: 117 --VSWYSKEEVLEKEPYAS-GDIFGASFIQDDVHVEPYFVCKAYVKAAKMLGAEIFEHTP 173
Query: 172 VKGFIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQ 231
V + +GE +KT G + VV A+ W+ + G+ + +V
Sbjct: 174 VL-HVERDGEALFIKTPSGDVWANHVVVASGVWSGMFFKQLGLNNAFLPVKGECLSVWND 232
Query: 232 PIKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKI 291
I P+ + + H Y+ G ++ G + G + TP L V +
Sbjct: 233 DI------PLTKTLYHDHCYIVPRKSGRLVVGATMKPGD-WSETPDLGGLESVMKKAKTM 285
Query: 292 IPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADL 351
+PA++N+ V R WAG T D P IG+ E S AAG +G ++APA G +I+DL
Sbjct: 286 LPAIQNMKVDRFWAGLRPGTKDGKPYIGRHPEDSRILFAAGHFRNGILLAPATGALISDL 345
Query: 352 ITKGKSKLPVEWYDPYRFERGE 373
I ++ +W +R +R E
Sbjct: 346 IM--NKEVNQDWLHAFRIDRKE 365
>pir||H83370 hydrogen cyanide synthase HcnC PA2195 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|6644364|gb|AAF21030.1|AF208523_3 (AF208523) HcnC
[Pseudomonas aeruginosa]
>gi|9948217|gb|AAG05583.1|AE004646_4 (AE004646) hydrogen
cyanide synthase HcnC [Pseudomonas aeruginosa]
Length = 417
Score = 104 bits (257), Expect = 2e-21
Identities = 93/408 (22%), Positives = 176/408 (42%), Gaps = 51/408 (12%)
Query: 7 EVVVIGGGIVGVSIAHELAKRG-VDVTVLEKR--------------FIGSGSTFRCGTGI 51
++V+ GGG++G S A++L++RG + + V++ + IG CG
Sbjct: 6 DIVIAGGGVIGASCAYQLSRRGNLRIAVVDDKRPGNATRASAGGLWAIGESVGLGCGVIF 65
Query: 52 RQQFNDEANVRVMKRSV--------------------------ELWKKYSEEY--NFSFK 83
+ + +V EL ++ E + +F F+
Sbjct: 66 FRMMSSRNRREAQGAAVAVDASTPHILPPAFFDLALQSNALYPELHRELIERHGMDFKFE 125
Query: 84 QTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIVPLLDISEVIAASWNPTD 143
+TG +++ D+E+ + + + R + EE + P + + A + D
Sbjct: 126 RTGLKYVIQDDEDRQYAEHIVAQIPHLAEQVRWLDREELRRAEPAVSHAAHGALEFL-CD 184
Query: 144 GKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEIKGVKT-NRGTIKTGIVVNATN 202
+ PF A+ AR+ G +LL T V G + + I GV+T N G + ++NA
Sbjct: 185 HQVSPFRLADAYLEAARQNGVELLPGTNVTGVLRQGRRISGVRTDNAGVLHCRTLINAAG 244
Query: 203 AWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPMVISFKYGHAYLTQTFHGGIIG 262
AWA ++ MA + +IP++P K Q V+T+ + R + S Y+ Q +G I+
Sbjct: 245 AWAAELSEMATGR-RIPVKPVKGQIVLTERMPRLLNGCLTTS----DCYMAQKDNGEILI 299
Query: 263 GIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIE 322
G E +D++ T+ + + + +P L+ + + RTWAG +PD P +G +
Sbjct: 300 GSTTE-DKGFDVSNTFPEIAGLVQGAVRCVPELQQVNLKRTWAGLRPGSPDELPILGPVA 358
Query: 323 ELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLPVEWYDPYRFE 370
E+ Y A G G + + G ++ L+ + L + + RF+
Sbjct: 359 EVEGYLNACGHFRTGILTSAITGVLLDRLVHEETLPLDIAPFLAARFQ 406
>gb|AAK42656.1| Glycerol-3-phosphate dehydrogenase chain A (glpA) [Sulfolobus
solfataricus]
Length = 445
Score = 104 bits (256), Expect = 2e-21
Identities = 107/379 (28%), Positives = 178/379 (46%), Gaps = 34/379 (8%)
Query: 5 RSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFR----CGTGIRQQFNDEAN 60
+S V VIGGG+ G+ A +LA RG+DVT++++ IG G++ + +G R D +
Sbjct: 17 KSTVTVIGGGVSGLFTALDLALRGIDVTLVDRGDIGYGTSGKFHGLLHSGARYAVTDPDS 76
Query: 61 VRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPE 120
R + ++ K + + K TG +FL +++++ + I+ NK G+ +++I +
Sbjct: 77 ARECIQENKIISKIAPH---TVKDTGGVFLGITDDDLQFSETFIKALNKVGIESKVIDVK 133
Query: 121 EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG 180
E + P ++ + A W P D ++ + AI A GAK+L Y EV I EN
Sbjct: 134 EVLQKEPFIN-RDTKMAIWVP-DKVVYGYDLLASVAITASLNGAKILTYNEVVEIIRENN 191
Query: 181 EIKGVK------TNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIK 234
+KGVK N IK+ I+VN W+ I MAG++ +IPI P V+ K
Sbjct: 192 NVKGVKVLDKINNNTNVIKSDIIVNTAGPWSFNIIKMAGLE-EIPIMPTAGIIVVFD--K 248
Query: 235 RGTINPMVISFKYGHA---YLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKI 291
R +N MVI+ + + I+G I + T + E + + +
Sbjct: 249 R--VNNMVINRLRPPSDGDIIVPYADSSILGTTATIIEDPDNFTISDEDIAMLVNEGAYL 306
Query: 292 IPALKNLLVLRTWAGY--YAKTPDSNPAIGKIEELSDYYIAAGFS------GHGFMMAPA 343
IP LKN+ V+R++A K+ S + + D+ G S G F
Sbjct: 307 IPKLKNMRVVRSYASVRPLIKSEVSAREASRDFRIIDHEKENGLSGLVSVIGGKFTTGRL 366
Query: 344 VGEMIADLITKGKSKLPVE 362
GE +ADL++ SKL ++
Sbjct: 367 AGERVADLVS---SKLGIK 382
>sp|P43799|GLPA_HAEIN ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT A (G-3-P
DEHYDROGENASE) >gi|1074156|pir||E64086
glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) chain
A, anaerobic - Haemophilus influenzae (strain Rd KW20)
>gi|1573687|gb|AAC22345.1| (U32751) anaerobic
glycerol-3-phosphate dehydrogenase, subunit A (glpA)
[Haemophilus influenzae Rd]
Length = 563
Score = 101 bits (248), Expect = 2e-20
Identities = 96/321 (29%), Positives = 150/321 (45%), Gaps = 36/321 (11%)
Query: 6 SEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFR----CGTGIRQQFNDEANV 61
++V++IGGG G IA + A RG+D +LE+R I +G+T R +G R ND+ +
Sbjct: 19 TDVIIIGGGATGAGIARDCALRGIDCILLERRDIATGATGRNHGLLHSGARYAVNDQESA 78
Query: 62 RVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEE 121
+ + +K + +T LF+ E+ ++ K +E K G+ + I PE
Sbjct: 79 EECIKENRILRKIARH---CVDETEGLFITLPEDSLDYQKTFLESCAKSGIDAQAIDPEL 135
Query: 122 AKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGE 181
AK + P ++ +++ A P DG DPF T + + A E GAK+ Y EVK I E G+
Sbjct: 136 AKIMEPSVN-PDLVGAVVVP-DGSIDPFRLTASNMMDATENGAKMFTYCEVKNLIREGGK 193
Query: 182 IKGVKT----NRGTIK--TGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKR 235
+ GV NR + +VVNA W + I A +K+K + P K + +
Sbjct: 194 VIGVNAYDHKNRRERQFFAPLVVNAGGIWGQGIAEYADLKIK--MFPAKGALL----VMG 247
Query: 236 GTINPMVISFKYGHAYLTQTFHGGIIGGIG-------YEIGPTYDLTPTYEFLREVSYYF 288
IN +VI+ A G I IG Y+ ++TP EV F
Sbjct: 248 HRINKLVINRCRKPADADILVPGDTICVIGTTSSRIPYDQIDNMEVTP-----EEVDILF 302
Query: 289 ---TKIIPALKNLLVLRTWAG 306
K+ P+L++ VLR +AG
Sbjct: 303 REGEKLAPSLRHTRVLRAYAG 323
>emb|CAB63337.1| (AL032631) contains similarity to Pfam domain: PF01571 (Glycine
cleavage T-protein (aminomethyl transferase)),
Score=250.7, E-value=6.4e-72, N=1~cDNA EST EMBL:Z14769
comes from this gene~cDNA EST EMBL:M89219 comes from
this gene~cDNA EST yk17f7.3 comes>
Length = 872
Score = 101 bits (248), Expect = 2e-20
Identities = 100/392 (25%), Positives = 163/392 (41%), Gaps = 43/392 (10%)
Query: 6 SEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQ---FNDEANVR 62
++VVV GGGI G SIA+ LAKRG V ++EK IG G+ + D +
Sbjct: 22 ADVVVCGGGISGTSIAYHLAKRGKKVALVEKDSIGCSGATGLSAGLVSSPIFWQDTSLQA 81
Query: 63 VMKRSVELWKKYSEEYNFSFKQTGYLFLLYD-EEEV---ETFKRNIEIQNKFGVPTRLIT 118
+ + S++L+ + F + + G +L E+ + R + +K + L
Sbjct: 82 IAQASLDLYSHLATTCKFRYIKCGRTYLASSMANEILLRRMYSRGVVHNDKVEL---LDC 138
Query: 119 PEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEV-KGFII 177
E E P L +V A ++P D DP A+ A++YGA + E V + I
Sbjct: 139 QSEMLERWPFLQTEDVQLALFSPEDVALDPVALCQHLALIAKDYGALIYESNPVLEVHIG 198
Query: 178 ENGEIKGVKTNRGTIKTGIVVNATNAWA--KLINAMAGIKVKIPIEPYKHQAVITQPIKR 235
+ ++ GV T G I+T V+A WA L+ A+ V+ P + + T +
Sbjct: 199 DEKQVYGVSTKMGFIETSHFVDAAGIWAGSHLVKALPHQHVQTAAYPCTYSYIHTSKLPT 258
Query: 236 GTINPMVISFK--YGHAYLTQTFHGGIIGGIGYE-IGP----TYDLTP------------ 276
G+++ M F G+ L T + G E I P T TP
Sbjct: 259 GSVSDMTPIFNDLDGNVMLRTTSFKTLCAGFAEESIRPLARQTGSATPWQHPEPDWNTFG 318
Query: 277 -TYEFLREVSYYFTKIIPALKNLLVLRTW----------AGYYAKTPDSNPAIGKIEELS 325
F +Y T P L+ L+ G A TPD P IG+ +
Sbjct: 319 MALRFTAGRQFYKTAYHPNLEKLISRCPMLGDCNHGDLIVGMEAYTPDKLPTIGESSQAK 378
Query: 326 DYYIAAGFSGHGFMMAPAVGEMIADLITKGKS 357
Y++ G +G G +A +G+++ +L+ + +S
Sbjct: 379 GYWVMCGMNGQGLSLAGGLGKILGELMCESQS 410
>gb|AAC38596.1| (AF053760) hydrogen cyanide synthase HcnC [Pseudomonas fluorescens]
Length = 417
Score = 99.5 bits (244), Expect = 6e-20
Identities = 94/407 (23%), Positives = 175/407 (42%), Gaps = 51/407 (12%)
Query: 7 EVVVIGGGIVGVSIAHELAKR-GVDVTVLEKRFIGSGST--------------------- 44
+VV+ GGG++G S A++L+KR + V +++ + G+ S
Sbjct: 6 DVVIAGGGVIGASCAYQLSKRKDLKVALIDAKRPGNASRASAGGLWAIGESVGLGCGVIF 65
Query: 45 FRCGTGIRQQ-------FNDEANVRVMKRSV--------ELWKKYSEEY------NFSFK 83
FR + R++ D + ++ +S EL+ + E +F F+
Sbjct: 66 FRMMSANRKREAQGSAVVVDSSTPHILPQSFFDFALQSNELYPRLHRELMGLHNMDFKFE 125
Query: 84 QTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIVPLLDISEVIAASWNPTD 143
QTG F++YDEE+ + + R + + P + E A D
Sbjct: 126 QTGLKFVIYDEEDRLYAEHIVGCIPHLSDQVRWLDQAALRASEPNVS-HEAQGALEFLCD 184
Query: 144 GKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEIKGVKTN-RGTIKTGIVVNATN 202
+ +PF T A+ AR+ G + T V G + + + GVKT+ G + ++NA
Sbjct: 185 HQVNPFRLTDAYTEGARQNGVDVYFNTNVTGVLHQGNRVSGVKTDVAGLFRCTTLINAAG 244
Query: 203 AWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPMVISFKYGHAYLTQTFHGGIIG 262
AWA ++ A ++IP++P K Q ++T+ + + + S Y+ Q +G I+
Sbjct: 245 AWAAELSLQA-TGIEIPVKPVKGQILLTERMPKLLNGCLTTS----DCYMAQKDNGEILI 299
Query: 263 GIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIE 322
G E +D+T TY + + + +P L ++ + R WAG +PD P +G ++
Sbjct: 300 GSTTE-DKGFDVTTTYPEINGLVQGAVRCVPELAHVNLKRCWAGLRPGSPDELPILGPMD 358
Query: 323 ELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLPVEWYDPYRF 369
+ Y A G G + + G ++ L+ + L + + RF
Sbjct: 359 GVEGYLNACGHFRTGILTSAITGVLLDKLVNEEALPLDITPFLARRF 405
>gb|AAG20146.1| (AE005092) glycerol-3-phosphate dehydrogenase chain A; GpdA2
[Halobacterium sp. NRC-1]
Length = 570
Score = 98.3 bits (241), Expect = 1e-19
Identities = 69/238 (28%), Positives = 118/238 (48%), Gaps = 28/238 (11%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFR----CGTGIRQQFND 57
+ + VVV+GGG G +A +LA RG+ VT++E+ + G+T R +G R +D
Sbjct: 1 MAQEPHVVVVGGGSTGAGVARDLAMRGLGVTLVEQGNLTHGTTGRMHGLLHSGGRYAVSD 60
Query: 58 EANVR--VMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTR 115
+A+ R +++ V E +TG LF+ E+ + F+ + +PT+
Sbjct: 61 QASARECIIENRV-----LREIATHCVDETGGLFVKRPEDSEQYFQEKLAGCEACDIPTK 115
Query: 116 LITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGF 175
+++ EA+++ P L +V A W P D DPF A A A+E+GA++ ++ V
Sbjct: 116 VLSGAEARQLEPHL-ADDVEKAIWVP-DAAIDPFRLVVANAASAQEHGARIETHSTVTDV 173
Query: 176 IIENGEIKGVKTNRGT---------------IKTGIVVNATNAWAKLINAMAGIKVKI 218
++E+G + GV+ T I+ VVNA AWA + MAG+ V++
Sbjct: 174 VVEDGAVVGVEVEHATGAGKRVHGTEGGTEVIRADHVVNAAGAWAGRLGEMAGVDVEV 231
>pir||JC7176 L-amino acid deaminase (EC 3.5.4.-) - Proteus vulgaris
>gi|7007412|dbj|BAA90864.1| (AB030003) L-amino acid
deaminase [Proteus vulgaris]
Length = 471
Score = 96.7 bits (237), Expect = 4e-19
Identities = 107/426 (25%), Positives = 177/426 (41%), Gaps = 63/426 (14%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFI-GSGSTFRCGTGIRQQFNDEAN 60
LP++++VVV+G GI+G+ A L +RG+ V ++EK I G S+ G I + DE
Sbjct: 51 LPKQADVVVVGAGILGIMTAINLVERGLSVVIVEKGNIAGEQSSRFYGQAISYKMPDETF 110
Query: 61 VRVMKRSVELWKKYSEEYNF--SFKQTGYLFLLYDEEEVETFKRNIEIQNK-----FGVP 113
+ + W++ + + +++ G + + DEE++ ++ I+ ++K
Sbjct: 111 L-LHHLGKHRWREMNAKVGIDTTYRTQGRVEVPLDEEDLVNVRKWIDERSKNVGSDIPFK 169
Query: 114 TRLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVK 173
TR+I E + + IA + G DP AT A A++ G ++ +
Sbjct: 170 TRIIEGAELNQRLRGATTDWKIAG-FEEDSGSFDPEVATFVMAEYAKKMGVRIYTQCAAR 228
Query: 174 GFIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEP-YKHQAVIT-Q 231
G + G I V T +G IKT VV A W++L M + V +P P Y+ Q +I+
Sbjct: 229 GLETQAGVISDVVTEKGAIKTSQVVVAGGVWSRLF--MQNLNVDVPTLPAYQSQQLISGS 286
Query: 232 PIKRG-------------------TINPMVI-------SFKYGHAYLTQ----------T 255
P G +P VI SF YG+ YL +
Sbjct: 287 PTAPGGNVALPGGIFFREQADGTYATSPRVIVAPVVKESFTYGYKYLPLLALPDFPVHIS 346
Query: 256 FHGGIIGGIGYEIGPTYDLTPTYEFLR---------EVSYYFTKI---IPALKNLLVLRT 303
+ +I D +E R E++ K+ PA K ++
Sbjct: 347 LNEQLINSFMQSTHWNLDEVSPFEQFRNMTALPDLPELNASLEKLKAEFPAFKESKLIDQ 406
Query: 304 WAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLPVEW 363
W+G A PD NP I +++E I +G G +P E+ ADL+ K L +
Sbjct: 407 WSGAMAIAPDENPIISEVKEYPGLVINTA-TGWGMTESPVSAELTADLLLGKKPVLDPKP 465
Query: 364 YDPYRF 369
+ YRF
Sbjct: 466 FSLYRF 471
>sp|P13032|GLPA_ECOLI ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT A (G-3-P
DEHYDROGENASE) >gi|65976|pir||DEECNA
glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) chain
A, anaerobic [validated] - Escherichia coli
>gi|1788574|gb|AAC75301.1| (AE000314)
sn-glycerol-3-phosphate dehydrogenase (anaerobic), large
subunit [Escherichia coli K12]
>gi|1799588|dbj|BAA16060.1| (D90855)
glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) chain
A, anaerobic [Escherichia coli]
Length = 542
Score = 87.8 bits (214), Expect = 2e-16
Identities = 60/225 (26%), Positives = 105/225 (46%), Gaps = 15/225 (6%)
Query: 4 ERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFR----CGTGIRQQFNDEA 59
+ S+V++IGGG G IA + A RG+ V ++E+ I +G+T R +G R D
Sbjct: 7 QSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAE 66
Query: 60 NVRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
+ R ++ K+ + + T LF+ E+++ I + G+ I P
Sbjct: 67 SARECISENQILKRIARH---CVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDP 123
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIEN 179
++A+ I P ++ + + DG DPF T A + A+E+GA +L EV G I E
Sbjct: 124 QQARIIEPA--VNPALIGAVKVPDGTVDPFRLTAANMLDAKEHGAVILTAHEVTGLIREG 181
Query: 180 GEIKGVKTNR------GTIKTGIVVNATNAWAKLINAMAGIKVKI 218
+ GV+ + +VVNA W + I A +++++
Sbjct: 182 ATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRM 226
>gb|AAA83864.1| (M20938) glycerol-3-phosphate dehydrogenase subunit A [Escherichia
coli]
Length = 542
Score = 87.8 bits (214), Expect = 2e-16
Identities = 60/225 (26%), Positives = 105/225 (46%), Gaps = 15/225 (6%)
Query: 4 ERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFR----CGTGIRQQFNDEA 59
+ S+V++IGGG G IA + A RG+ V ++E+ I +G+T R +G R D
Sbjct: 7 QSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAE 66
Query: 60 NVRVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
+ R ++ K+ + + T LF+ E+++ I + G+ I P
Sbjct: 67 SARECISENQILKRIARH---CVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDP 123
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIEN 179
++A+ I P ++ + + DG DPF T A + A+E+GA +L EV G I E
Sbjct: 124 QQARIIEPA--VNPALIGAVKVPDGTVDPFRLTAANMLDAKEHGAVILTAHEVTGLIREG 181
Query: 180 GEIKGVKTNR------GTIKTGIVVNATNAWAKLINAMAGIKVKI 218
+ GV+ + +VVNA W + I A +++++
Sbjct: 182 ATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRM 226
>pir||H70361 conserved hypothetical protein aq_707 - Aquifex aeolicus
>gi|2983300|gb|AAC06892.1| (AE000703) hypothetical
protein [Aquifex aeolicus]
Length = 353
Score = 87.4 bits (213), Expect = 3e-16
Identities = 102/379 (26%), Positives = 158/379 (40%), Gaps = 43/379 (11%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTVL--EKR----FIGSGSTFRCGTGIRQQFNDEAN 60
E++VIGGG++G+SI EL G DV +L +KR ++ G G+R F
Sbjct: 2 EILVIGGGVIGLSIGVELLLAGHDVRLLVRDKREGASWVAGGMLAPFSEGLRGDF----- 56
Query: 61 VRVMKRSVELWKKYSEEY-----NFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTR 115
+R S+ LW Y E F G L + +DE+E + + +++ ++ V
Sbjct: 57 LRFSVESLRLWHDYLERLKEISGRKVFFSQGILRVAFDEKEEKKLREDVKFYSQ-EVCQN 115
Query: 116 LIT--PEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVK 173
LI+ EE + P L SEV +G D E A + + G +++ VK
Sbjct: 116 LISYDAEELLKEFPYLS-SEVRYGVIYGDEGNVDTEELMEALYVAFEKLGGEIINEDAVK 174
Query: 174 GFIIENGE-IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAV-ITQ 231
I+ NGE I+ V V AT AW K P+ P K Q + +
Sbjct: 175 --ILRNGERIEKVFGFFEEFTADYYVFATGAWLK-------EHFGFPVFPVKGQILRVEA 225
Query: 232 PIKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKI 291
P+K + AY+ +IG G +D T E ++++S
Sbjct: 226 PLKDYVVYS-------SRAYIIPREKDLLIGATTENAG--FDTKTTLEGVKKLSEGAISS 276
Query: 292 IPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADL 351
+P LK +L G+ TPD P E S Y G +G ++APA E+ L
Sbjct: 277 VPQLKEAQLLEVRVGFRPGTPDELPVFYFGENFSVY---GGHYRNGILLAPATAEVALKL 333
Query: 352 ITKGKSKLPVEWYDPYRFE 370
I +G+ + + PYRF+
Sbjct: 334 IDQGEVSEYFKTFSPYRFK 352
>gb|AAA86752.1| (U35383) amino acid deaminase [Proteus mirabilis]
Length = 473
Score = 84.3 bits (205), Expect = 2e-15
Identities = 107/425 (25%), Positives = 170/425 (39%), Gaps = 60/425 (14%)
Query: 2 LPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANV 61
LP+ ++VV+IG GI G+ A LA+RG+ VT+LEK I + R + I
Sbjct: 51 LPKEADVVIIGAGIQGIMTAINLAERGMSVTILEKGQIAGEQSGRAYSQIISYQTSPEIF 110
Query: 62 RVMKRSVELWKKYSEEY--NFSFKQTGYLFLLYDEEEVETFKRNIEIQNK---FGVP--T 114
+ LW+ +E+ + S++ G + L DE+ ++ + I+ + F P T
Sbjct: 111 PLHHYGKILWRGMNEKIGADTSYRTQGRVEALADEKALDKAQAWIKTAKEAAGFDTPLNT 170
Query: 115 RLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKG 174
R+I EE + +AA + G DP T A A A++ G K+ V+G
Sbjct: 171 RIIKGEELSNRLVGAQTPWTVAA-FEEDSGSVDPETGTPALARYAKQIGVKIYTNCAVRG 229
Query: 175 FIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQP-I 233
G+I V + +G IKT VV A W++L GI + Q V P
Sbjct: 230 IETAGGKISDVVSEKGAIKTSQVVLAGGIWSRLFMGNMGIDIPTLNVYLSQQRVSGVPGA 289
Query: 234 KRGTIN-PMVISFK-------------YGHAYLTQTFHGG-----IIGG----IGYEIG- 269
RG ++ P I F+ + + + +F G ++GG + + IG
Sbjct: 290 PRGNVHLPNGIHFREQADGTYAVAPRIFTSSIVKDSFLLGPKFMHLLGGGELPLEFSIGE 349
Query: 270 --------PTY----DLTPTYEF-----------LREVSYYFTKIIPALKNLLVLRTWAG 306
PT + TP +F L V P + V+ W
Sbjct: 350 DLFNSFKMPTSWNLDEKTPFEQFRVATATQNTQHLDAVFQRMKTEFPVFEKSEVVERWGA 409
Query: 307 YYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKLPVEWYDP 366
+ T D P I +++E I + G PA GE+ AD++ K PV P
Sbjct: 410 VVSPTFDELPIISEVKEYPGLVINTA-TVWGMTEGPAAGEVTADIV---MGKKPVIDPTP 465
Query: 367 YRFER 371
+ +R
Sbjct: 466 FSLDR 470
>pir||C82001 D-amino-acid dehydrogenase (EC 1.4.99.1) small subunit NMA0092
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7378865|emb|CAB83408.1| (AL162752) D-amino
acid dehydrogenase small subunit [Neisseria meningitidis
Z2491]
Length = 418
Score = 84.3 bits (205), Expect = 2e-15
Identities = 96/406 (23%), Positives = 165/406 (39%), Gaps = 66/406 (16%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTV--------LEKRFIGSGST-------------- 44
+V+V+G G+ GVS A LA+ G +VTV +E F +G
Sbjct: 2 KVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANAGQLSYGYTTPWAAPGIP 61
Query: 45 ---------------FRCG----------------TGIRQQFNDEANVRVMKRSVELWKK 73
FR T R Q N E VR+ + S E++++
Sbjct: 62 TKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQHCTAARYQINKERMVRMSEYSREMFRR 121
Query: 74 YSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIVP-LLD 130
+ + NF ++ G L + +EVE K++I + ++GVP R + PEE E P L
Sbjct: 122 FEAQTGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERYGVPYRRLKPEECAEFEPALAR 181
Query: 131 ISEVIAASWN-PTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG-EIKGVKTN 188
++ IA + P D D T +E G + + + I NG IK V+T
Sbjct: 182 VTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVR-FHFNQTISRIDHNGLRIKTVETE 240
Query: 189 RGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPMVISFKYG 248
G + VV A +++ + +A + + +PI P K + +T P+ P+
Sbjct: 241 TGRFEADAVVCALGCFSRTV--LAQVDLNLPIYPVKGYS-LTLPVTNSDGAPVSTVLDES 297
Query: 249 HAYLTQTFHGGI-IGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLVLRTWAGY 307
+ F+ I +GG+ G Y + + ++ + P +L W+G
Sbjct: 298 YKVAITRFNNRIRVGGMAELSG--YAIKLPEKRRETLALVVNDLFPEGGDLNQTLFWSGL 355
Query: 308 YAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLIT 353
TPDS P IG+ + ++ G G+ M+ ++ AD+++
Sbjct: 356 RPMTPDSTPLIGR-TRFDNLFLNTGHGTLGWTMSLGSAKLTADIVS 400
>sp|O32159|YURR_BACSU HYPOTHETICAL 39.4 KD OXIDOREDUCTASE IN HOM-MRGA INTERGENIC REGION
>gi|7475684|pir||A70019 opine catabolism homolog yurR -
Bacillus subtilis >gi|2635760|emb|CAB15253.1| (Z99120)
similar to opine catabolism [Bacillus subtilis]
Length = 372
Score = 83.5 bits (203), Expect = 4e-15
Identities = 93/385 (24%), Positives = 155/385 (40%), Gaps = 49/385 (12%)
Query: 9 VVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGT---GIRQQFNDE------A 59
+++G GI+G S A+ LAK G VTV++++ G + G + Q+ N +
Sbjct: 5 IIVGAGILGASTAYHLAKTGARVTVIDRKEPGQATDAAAGIVCPWLSQRRNQDWYQLAKG 64
Query: 60 NVRVMKRSVELWKKYSEEYNFSFKQTGYLFL-----LYDEEEVETFKRNIEIQNKFGVPT 114
R K + +K E + +K+ G + + D+ E +KR E + G T
Sbjct: 65 GARYYKDLIHQLEKDGES-DTGYKRVGAISIHTDASKLDKMEERAYKRR-EDAPEIGDIT 122
Query: 115 RLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEA---TTAFAIKAREYGAKLLEYTE 171
RL + E K++ P+L AD +E+ + A + R LL E
Sbjct: 123 RL-SASETKKLFPIL----------------ADGYESVHISGAARVNGRALCRSLLSAAE 165
Query: 172 VKG---------FIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEP 222
+G + ENG + GV+T+ V+ AWA I GI ++ +
Sbjct: 166 KRGATVIKGNASLLFENGTVTGVQTDTKQFAADAVIVTAGAWANEILKPLGIHFQVSFQ- 224
Query: 223 YKHQAVITQPIKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYD-LTPTYEFL 281
K Q + + T + V+ Y+ +G I+ G +E D L T
Sbjct: 225 -KAQIMHFEMTDADTGSWPVV-MPPSDQYILSFDNGRIVAGATHENDAGLDDLRVTAGGQ 282
Query: 282 REVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMA 341
EV + P L + + T G+ TP P +G + + Y A G G M
Sbjct: 283 HEVLSKALAVAPGLADAAAVETRVGFRPFTPGFLPVVGAVPNVQGLYAANGLGASGLTMG 342
Query: 342 PAVGEMIADLITKGKSKLPVEWYDP 366
P +G +A L+ +++L + YDP
Sbjct: 343 PFLGAELAKLVLGKQTELDLSPYDP 367
>pir||B83078 probable D-amino acid oxidase PA4548 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9950792|gb|AAG07936.1|AE004868_7 (AE004868) probable
D-amino acid oxidase [Pseudomonas aeruginosa]
Length = 364
Score = 83.1 bits (202), Expect = 5e-15
Identities = 85/354 (24%), Positives = 156/354 (44%), Gaps = 19/354 (5%)
Query: 8 VVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVMKRS 67
VVV+G G++G+ A ELA G+ VT++E+ G +++ G + + + V +
Sbjct: 5 VVVVGAGVIGLLTARELALAGLRVTLVERGESGREASWAGGGIVSPLYPWRYSPAVTALA 64
Query: 68 VELWKKYSEEYNFSFKQTGY--------LFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
Y +TG L+ L +++ E ++ K P + +
Sbjct: 65 HWSQDFYPALGQRLLDETGLDPEVHTVGLYWLDLDDQTEA----LQWARKHTRPLKEVPI 120
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIEN 179
EEA VP L A + +P A + A + +L E TEV+G++ +
Sbjct: 121 EEAYAAVPGLGAGFQRAVYMSGVANVRNPRLARSLRASLQQFANLELHEQTEVRGWLRDG 180
Query: 180 GEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTIN 239
+ GV T+RG I+ V+ A AW+ + G++ +P+ P K Q ++ +
Sbjct: 181 DRVVGVATSRGEIRGDKVLLAAGAWSGELLKPLGLE--LPVVPVKGQMILYKCA--ADFL 236
Query: 240 PMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLL 299
P ++ K +A + H +IG G +D TPT E + +++P L ++
Sbjct: 237 PRMVLAKGRYAIPRRDGHI-LIGSTLEHSG--FDKTPTDEAQESLRASAAELLPELADMQ 293
Query: 300 VLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLIT 353
+ WAG +P+ P IG + ++ G +G ++APA ++ADL++
Sbjct: 294 PVAHWAGLRPGSPEGIPYIGPVPGFDGLWLNTGHYRNGLVLAPASCRLLADLMS 347
>sp|P33642|YFIT_PSEAE HYPOTHETICAL 39.5 KD OXIDOREDUCTASE IN FIMT 3'REGION (DADA*) (ORFZ)
>gi|1161219|gb|AAB39269.1| (L48934) homolgous to D-amino
acid dehydrogenase enzyme [Pseudomonas aeruginosa]
Length = 364
Score = 83.1 bits (202), Expect = 5e-15
Identities = 85/354 (24%), Positives = 156/354 (44%), Gaps = 19/354 (5%)
Query: 8 VVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEANVRVMKRS 67
VVV+G G++G+ A +LA G+ VT++E+ G +++ G + + N V +
Sbjct: 5 VVVVGAGVIGLLTARKLAIAGLRVTLVERGESGREASWAGGGIVSPLYPWRYNPAVTALA 64
Query: 68 VELWKKYSEEYNFSFKQTGY--------LFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
Y +TG L+ L +++ E ++ K P + +
Sbjct: 65 HWSQDFYPALGQRLLDETGLDPEVHTVGLYWLDLDDQTEA----LQWAPKHTRPLKEVPI 120
Query: 120 EEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIEN 179
EEA VP L A + +P A + A + +L E TEV+G++ +
Sbjct: 121 EEAYAAVPGLGAGFQRAVYMSGVANVRNPRLARSLRASLQQFANLELHEQTEVRGWLRDG 180
Query: 180 GEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTIN 239
+ GV T+RG I+ V+ A AW+ + G++ +P+ P K Q ++ +
Sbjct: 181 DRVVGVATSRGEIRGDKVLLAAGAWSGELLKPLGLE--LPVVPVKGQMILYKCA--ADFL 236
Query: 240 PMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLL 299
P ++ K +A + H +IG G +D TPT E + +++P L ++
Sbjct: 237 PRMVLAKGRYAIPRRDGHI-LIGSTLEHSG--FDKTPTDEAQESLRVSVAELLPELADMQ 293
Query: 300 VLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLIT 353
+ WAG +P+ P IG + ++ G +G ++APA ++ADL++
Sbjct: 294 PVAHWAGLRPGSPEGIPYIGPVPGFDGLWLNTGHYRNGLVLAPASCRLLADLMS 347
>sp|Q52671|SAOX_RHOCA SARCOSINE OXIDASE BETA SUBUNIT (SARCOSINE OXIDASE SUBUNIT B)
>gi|1353872|gb|AAB01814.1| (U46071) sarcosine oxidase
[Rhodobacter capsulatus]
Length = 208
Score = 82.7 bits (201), Expect = 6e-15
Identities = 55/177 (31%), Positives = 93/177 (52%), Gaps = 8/177 (4%)
Query: 4 ERSEVVVIGGGIVGVSIAHELAK-RGV-DVTVLEKRFIGSGSTFRCGTGIRQQFNDEANV 61
+R +V+V+G G G++ A+ L K G+ +V ++EK ++G G+T R T IR + + +
Sbjct: 31 KRYDVIVVGAGGHGLATAYYLGKVHGIKNVAIIEKGWLGGGNTGRNTTIIRSNYLQDPSA 90
Query: 62 RVMKRSVELWKKYSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITP 119
+ +++ L++ S++ YN F G L L E E+ +KR + N V TR I P
Sbjct: 91 AIYEKARSLYEGLSQDLNYNVMFSPRGLLMLAQSEHEMRGYKRTVYANNLQNVETRWIEP 150
Query: 120 EEAKEIVPLLDIS----EVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEV 172
+E K++VP+L+I+ V+ A G A +A + G LL+ TEV
Sbjct: 151 KEVKKLVPILNIAGPRYPVLGALLQEKGGTARHDAVAWGYARACSDMGMDLLQNTEV 207
>pir||A81228 D-amino acid dehydrogenase, small chain NMB0176 [imported] -
Neisseria meningitidis (group B strain MD58)
>gi|7225395|gb|AAF40633.1| (AE002375) D-amino acid
dehydrogenase, small subunit [Neisseria meningitidis
MC58]
Length = 418
Score = 82.3 bits (200), Expect = 8e-15
Identities = 95/406 (23%), Positives = 165/406 (40%), Gaps = 66/406 (16%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTV--------LEKRFIGSGST-------------- 44
+V+V+G G+ GVS A LA+ G +VTV +E F +G
Sbjct: 2 KVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANAGQLSYGYTTPWAAPGIP 61
Query: 45 ---------------FRCGTGIRQ----------------QFNDEANVRVMKRSVELWKK 73
FR + Q Q N E VR+ + S E++++
Sbjct: 62 TKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQTNKERMVRISEYSREMFRR 121
Query: 74 YSEE--YNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIVP-LLD 130
+ + NF ++ G L + +EVE K++I + ++GVP R + PEE E P L
Sbjct: 122 FEAQTGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERYGVPYRRLKPEECAEFEPALAR 181
Query: 131 ISEVIAASWN-PTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG-EIKGVKTN 188
++ IA + P D D T +E G + + + I NG IK V+T
Sbjct: 182 VTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVR-FHFNQNISRIDHNGLRIKTVETE 240
Query: 189 RGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINPMVISFKYG 248
G + VV A +++ + +A + + +PI P K + +T P+ P+
Sbjct: 241 TGRFEADAVVCALGCFSRTV--LAQLDLNLPIYPVKGYS-LTLPVTNSDGAPVSTVLDES 297
Query: 249 HAYLTQTFHGGI-IGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLVLRTWAGY 307
+ F I +GG+ G Y++ + ++ + P +L W+G
Sbjct: 298 YKVAITRFDNRIRVGGMAELSG--YEIKLPEKRRETLALVVNDLFPEGGDLSQASFWSGL 355
Query: 308 YAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLIT 353
TPDS P IG+ + ++ G G+ M+ ++ AD+++
Sbjct: 356 RPMTPDSTPLIGR-TRFDNLFLNTGHGTLGWTMSLGSAKLTADIVS 400
>pir||S76505 hypothetical protein - Synechocystis sp. (strain PCC 6803)
>gi|1001620|dbj|BAA10351.1| (D64002) ThiG protein
[Synechocystis sp.]
Length = 656
Score = 81.1 bits (197), Expect = 2e-14
Identities = 91/373 (24%), Positives = 161/373 (42%), Gaps = 28/373 (7%)
Query: 6 SEVVVIGGGIVGVSIAHELA--KRGVDVTVLEKRFIGSGSTFRCG--TGIRQQFNDEANV 61
S+V++IGGGI+G++IA EL ++ + VTVL + F + S G +Q +
Sbjct: 5 SDVLIIGGGIIGLAIAVELKLKQKRLQVTVLSRDFAQAASHAAAGMLAPHAEQIAPGPML 64
Query: 62 RVMKRSVELWKKYSEEYN-FSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPE 120
+ S + ++ E+ + +TGY E N + + + E
Sbjct: 65 DLCLASRWRYGEWVEKLEQLTGMETGYNPCGILSPVFEAPHGNSSTNSAW------LDQE 118
Query: 121 EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG 180
+ P L +VI W+P DG+ D + +A A+ G ++ E V+ +G
Sbjct: 119 TIRYYQPGLG-EDVIGGWWHPDDGQVDNRKLVSALRQAAQSLGVQIQEGVTVQAIAQRHG 177
Query: 181 EIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP 240
++ V T++G+ + V A +WAK + + +P+ P K Q + + + GT P
Sbjct: 178 QVTAVLTDQGSFQADSYVLANGSWAKEL-------LPLPVFPVKGQMMALR-MPAGTHQP 229
Query: 241 MVIS-FKYG-HAYLTQTFHGGIIGGIGYEIGPTYDLTP--TYEFLREVSYYFTKIIPALK 296
+ +G YL G +I G E D P T + ++ + ++ PAL
Sbjct: 230 YPLQRVLFGPQTYLVPRRDGRLIVGATSE---QVDWQPHNTPQGIQTLLGRAIRLFPALG 286
Query: 297 NLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGK 356
+ + W G+ TPD P +G + +A G +G ++AP +I+DLI K
Sbjct: 287 DWAIEDFWWGFRPGTPDEQPFLG-YGPCDNLILAIGHYRNGILLAPITAALISDLILDQK 345
Query: 357 SKLPVEWYDPYRF 369
+ + P RF
Sbjct: 346 VSPLIHAFSPQRF 358
>gb|AAA96395.1| (U38892) N-terminal and C-terminal ends are similar to the proteins
ThiG and D-amino acid dehydrogenase small subunits from
Escherichia coli, respectively; Method: conceptual
translation supplied by author; ORF656 [Synechocystis
sp.]
Length = 656
Score = 81.1 bits (197), Expect = 2e-14
Identities = 91/373 (24%), Positives = 161/373 (42%), Gaps = 28/373 (7%)
Query: 6 SEVVVIGGGIVGVSIAHELA--KRGVDVTVLEKRFIGSGSTFRCG--TGIRQQFNDEANV 61
S+V++IGGGI+G++IA EL ++ + VTVL + F + S G +Q +
Sbjct: 5 SDVLIIGGGIIGLAIAVELKLKQKRLQVTVLSRDFAQAASHAAAGMLAPHAEQIAPGPML 64
Query: 62 RVMKRSVELWKKYSEEYN-FSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPE 120
+ S + ++ E+ + +TGY E N + + + E
Sbjct: 65 DLCLASRWRYGEWVEKLEQLTGMETGYNPCGILSPVFEAPHGNSSTNSAW------LDQE 118
Query: 121 EAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENG 180
+ P L +VI W+P DG+ D + +A A+ G ++ E V+ +G
Sbjct: 119 TIRYYQPGLG-EDVIGGWWHPDDGQVDNRKLVSALRQAAQSLGVQIQEGVTVQAIAQRHG 177
Query: 181 EIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP 240
++ V T++G+ + V A +WAK + + +P+ P K Q + + + GT P
Sbjct: 178 QVTAVLTDQGSFQADSYVLANGSWAKEL-------LPLPVFPVKGQMMALR-MPAGTHQP 229
Query: 241 MVIS-FKYG-HAYLTQTFHGGIIGGIGYEIGPTYDLTP--TYEFLREVSYYFTKIIPALK 296
+ +G YL G +I G E D P T + ++ + ++ PAL
Sbjct: 230 YPLQRVLFGPQTYLVPRRDGRLIVGATSE---QVDWQPHNTPQGIQTLLGRAIRLFPALG 286
Query: 297 NLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGK 356
+ + W G+ TPD P +G + +A G +G ++AP +I+DLI K
Sbjct: 287 DWAIEDFWWGFRPGTPDEQPFLG-YGPCDNLILAIGHYRNGILLAPITAALISDLILDQK 345
Query: 357 SKLPVEWYDPYRF 369
+ + P RF
Sbjct: 346 VSPLIHAFSPQRF 358
>emb|CAC11717.1| (AL445064) sarcosine oxidase related protein [Thermoplasma
acidophilum]
Length = 427
Score = 79.2 bits (192), Expect = 7e-14
Identities = 102/418 (24%), Positives = 166/418 (39%), Gaps = 68/418 (16%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVD--VTVLEK-RFIGSGSTFRCGTGIRQQFNDEANVRV 63
+V +IG GIVG+S A L+++ D + V++K G+T + G R F+ + + ++
Sbjct: 14 DVAIIGSGIVGLSTAFHLSEKHSDLKIAVIDKFHTFAQGNTGKSAAGFRDVFSSDTSFKL 73
Query: 64 MKRSVELWKKYSE--EYNFSFKQTGYLFLLYDE---EEVETFKRNIEIQN-------KFG 111
S+ + + + K GYLFL+ ++ + +E R +I+ G
Sbjct: 74 SSSSIRFYDHVQKILGIDLGMKHVGYLFLMDNDSGSDVMEEIGRKTKIEEVDLDALESMG 133
Query: 112 VPTRLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTE 171
+ + T E KE + L DI A G +P + + + + L TE
Sbjct: 134 ISIKPDT--EVKEAMGLEDIHRAYLAH---NAGIMEPDKIAAFYHSQCVNRNIEFLFDTE 188
Query: 172 VKG---------------FIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKV 216
V+ FI ++ I +KT+RG I + AT+ W + GI
Sbjct: 189 VQSLNLVPKKPLNYPGEPFIWQDKTIGSIKTSRGEISADTFILATDVWTNFLTDPVGIDS 248
Query: 217 KIPIEPYKHQA----------VITQPIKRGTINPMVISFKYGHAYLT---QTFHGGIIGG 263
I P K Q V+ + + P + K + + +TF G+
Sbjct: 249 H--IRPKKRQLFKIRNEFIEDVVGKKVLATESFPFTVFPKGVYVRPSPGEKTFWAGVADD 306
Query: 264 IGYEIGPTYDLTP-----TYEFLREVSYYFTKIIPALKNLLVLRTWAGYYA-KTPDSNPA 317
IG + D TY + + Y I A + WAGYY+ T D NP
Sbjct: 307 IGRDFSFVEDPEAEPQFYTYNVYQVLQAYMKPITSA----SITGMWAGYYSYNTIDGNPY 362
Query: 318 IGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLIT-KGKSKLPVEWYDPYRFERGEL 374
I + L +A G SG G M A+G ++A L + K+KL YD R +L
Sbjct: 363 IFRDLNL---IVATGTSGSGIMKGDAIGRVVAALYDGEEKAKL----YDGMRINTADL 413
>pir||E82422 anaerobic glycerol-3-phosphate dehydrogenase, chain A VCA0747
[imported] - Vibrio cholerae (group O1 strain N16961)
>gi|9658168|gb|AAF96645.1| (AE004403) anaerobic
glycerol-3-phosphate dehydrogenase, subunit A [Vibrio
cholerae]
Length = 547
Score = 76.5 bits (185), Expect = 5e-13
Identities = 58/223 (26%), Positives = 103/223 (46%), Gaps = 15/223 (6%)
Query: 6 SEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFR----CGTGIRQQFNDEANV 61
++VV+IGGG G I + A RG+ ++EK I +G+T R +G R D +
Sbjct: 9 TDVVIIGGGATGTGILRDCALRGLRCILVEKDDIAAGTTGRNHGLLHSGARYAVTDSESA 68
Query: 62 RVMKRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEE 121
R + ++ K + + T LF+ E+++ + I + G+ T+ +TP+E
Sbjct: 69 RECIQENKILKSIARR---CVEATDGLFITLPEDDLGFQESFIHACSDAGIETQRLTPKE 125
Query: 122 AKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGE 181
A + P +++ + + DG DPF A + A+E+GA++ T V I E
Sbjct: 126 ALLLEP--NVNPALIGAVKVPDGTLDPFRLCAANVLDAKEHGARMFNRTIVTQLIREGDT 183
Query: 182 IKGVKTN------RGTIKTGIVVNATNAWAKLINAMAGIKVKI 218
+ GV+ + I V+NA W + I A + +K+
Sbjct: 184 VLGVRCQHLGSGAKFDIFAQQVINAAGIWGQNICEYADLNIKM 226
>sp|P40854|SAOX_STRSQ SARCOSINE OXIDASE >gi|80855|pir||JS0671 sarcosine oxidase (EC
1.5.3.1) precursor - Streptomyces sp
>gi|217024|dbj|BAA01473.1| (D10623) sarcosine oxidase
[Streptomyces sp.]
Length = 389
Score = 74.5 bits (180), Expect = 2e-12
Identities = 93/386 (24%), Positives = 153/386 (39%), Gaps = 37/386 (9%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTVLEKRF-IGSGSTFRCGTGIRQQ--FNDEANVRV 63
+V+VIG G +G + AH L+ RG V LEK + + + G+ I +Q F D A V +
Sbjct: 6 DVIVIGLGGMGSAAAHHLSARGARVLGLEKFGPVHNRGSSHGGSRITRQSYFEDPAYVPL 65
Query: 64 MKRSVELWKKYSEEYNFSFKQTGYLF--LLYDEEEVETFKRNIEIQNKFGVPTRLITPEE 121
+ R+ EL Y E + + L ++ + T ++ ++ + ++ +E
Sbjct: 66 LLRAYEL---YEELERATGRNVATLCGGVMAGPPDSRTVSGSLRSATEWDLAHEMLDAKE 122
Query: 122 AKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGE 181
+ P L + A + G P A A GA+L V +
Sbjct: 123 IRRRFPTLAPDDDEVALFEAKAGLLRPENMVAAHLQLATRQGAELRFEEPVLRWEPYRDG 182
Query: 182 IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKI---------------PIEPYKHQ 226
++ V T T G +V AWA + A G+ + + P P +H
Sbjct: 183 VR-VHTGENTYTAGQLVICPGAWAPQLLADIGVPITVERQIMYWFQPKGGTGPFVPERHP 241
Query: 227 AVITQPIKRGTINPMVISFKYGHAYLTQTFHGGIIGGI--GYEIGP-TYDLTPTYEFLRE 283
I + + YG + G + G P T D T +R
Sbjct: 242 VYIWEDADGVQV--------YGFPAIDGPEKGAKVAFFRKGQHTTPETIDRTVHAHEVRA 293
Query: 284 VSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYY-IAAGFSGHGFMMAP 342
++ + + +IP L L+ Y+ TPD + I + + +A GFSGHGF P
Sbjct: 294 MADHMSALIPDLPGTF-LKAATCMYSNTPDEHFVIARHPAHPESVTVACGFSGHGFKFVP 352
Query: 343 AVGEMIADLITKGKSKLPVEWYDPYR 368
VGE++ADL G + P+ +DP R
Sbjct: 353 VVGEILADLALTGATAHPIGLFDPAR 378
>gb|AAC60412.1| (S39464) sarcosine oxidase {EC 1.5.3.1} [Streptomyces sp.,
KB210-8SY, Peptide, 389 aa]
Length = 389
Score = 74.5 bits (180), Expect = 2e-12
Identities = 93/386 (24%), Positives = 153/386 (39%), Gaps = 37/386 (9%)
Query: 7 EVVVIGGGIVGVSIAHELAKRGVDVTVLEKRF-IGSGSTFRCGTGIRQQ--FNDEANVRV 63
+V+VIG G +G + AH L+ RG V LEK + + + G+ I +Q F D A V +
Sbjct: 6 DVIVIGLGGMGSAAAHHLSARGARVLGLEKFGPVHNRGSSHGGSRITRQSYFEDPAYVPL 65
Query: 64 MKRSVELWKKYSEEYNFSFKQTGYLF--LLYDEEEVETFKRNIEIQNKFGVPTRLITPEE 121
+ R+ EL Y E + + L ++ + T ++ ++ + ++ +E
Sbjct: 66 LLRAYEL---YEELERATGRNVATLCGGVMAGPPDSRTVSGSLRSATEWDLAHEMLDAKE 122
Query: 122 AKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGE 181
+ P L + A + G P A A GA+L V +
Sbjct: 123 IRRRFPTLAPDDDEVALFEAKAGLLRPENMVAAHLQLATRQGAELRFEEPVLRWEPYRDG 182
Query: 182 IKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKI---------------PIEPYKHQ 226
++ V T T G +V AWA + A G+ + + P P +H
Sbjct: 183 VR-VHTGENTYTAGQLVICPGAWAPQLLADIGVPITVERQIMYWFQPKGGTGPFVPERHP 241
Query: 227 AVITQPIKRGTINPMVISFKYGHAYLTQTFHGGIIGGI--GYEIGP-TYDLTPTYEFLRE 283
I + + YG + G + G P T D T +R
Sbjct: 242 VYIWEDADGVQV--------YGFPAIDGPEKGAKVAFFRKGQHTTPETIDRTVHAHEVRA 293
Query: 284 VSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYY-IAAGFSGHGFMMAP 342
++ + + +IP L L+ Y+ TPD + I + + +A GFSGHGF P
Sbjct: 294 MADHMSALIPDLPGTF-LKAATCMYSNTPDEHFVIARHPAHPESVTVACGFSGHGFKFVP 352
Query: 343 AVGEMIADLITKGKSKLPVEWYDPYR 368
VGE++ADL G + P+ +DP R
Sbjct: 353 VVGEILADLALTGATAHPIGLFDPAR 378
>sp|P29011|DADA_ECOLI D-AMINO ACID DEHYDROGENASE SMALL SUBUNIT >gi|1073354|pir||B53383
D-amino-acid dehydrogenase (EC 1.4.99.1) small chain -
Escherichia coli >gi|145703|gb|AAC36880.1| (L02948)
D-amino acid dehydrogenase [Escherichia coli]
>gi|1651591|dbj|BAA36044.1| (D90753) D-amino acid
dehydrogenase small subunit (EC 1.4.99.1). [Escherichia
coli] >gi|1787438|gb|AAC74273.1| (AE000217) D-amino acid
dehydrogenase subunit [Escherichia coli K12]
Length = 432
Score = 73.7 bits (178), Expect = 3e-12
Identities = 81/318 (25%), Positives = 132/318 (41%), Gaps = 14/318 (4%)
Query: 61 VRVMKRSVELWKKYSEEYNFSF--KQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLIT 118
VR+ + S + K E N + +Q G L L E++ E R+I + GVP +L+
Sbjct: 109 VRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLE 168
Query: 119 PEEAKEIVPLL-DISEVIAASWN-PTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFI 176
E+ P L +++ + P D D T A A + G K T V +
Sbjct: 169 SSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLL 228
Query: 177 IENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRG 236
+ +I GVK IK V A +++ + + GI V IP+ P K + +T PI +
Sbjct: 229 CDGEQIYGVKCGDEVIKADAYVMAFGSYSTAM--LKGI-VDIPVYPLKGYS-LTIPIAQE 284
Query: 237 TINPMVISFKYGHAYLTQTFHGGI-IGGIGYEIG-PTYDLTPTYEFLREVSYYFTKIIPA 294
P+ + F I +GG+ +G T L P E L V + P
Sbjct: 285 DGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV---VRDLYPR 341
Query: 295 LKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITK 354
++ W G TPD P +G+ + ++ G G+ MA G++++DL++
Sbjct: 342 GGHVEQATFWTGLRPMTPDGTPVVGRTR-FKNLWLNTGHGTLGWTMACGSGQLLSDLLSG 400
Query: 355 GKSKLPVEWYDPYRFERG 372
+P E R+ RG
Sbjct: 401 RTPAIPYEDLSVARYSRG 418
>gb|AAG09254.1|AF176664_3 (AF176664) iminodiacetate oxidase [EDTA-degrading bacterium BNC1]
Length = 371
Score = 73.4 bits (177), Expect = 4e-12
Identities = 85/381 (22%), Positives = 152/381 (39%), Gaps = 28/381 (7%)
Query: 8 VVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFRCGTGIRQQFNDEAN----VRV 63
V++IG GI+G S A+ LA+ G V ++++ G + G G+ + EA+ +
Sbjct: 3 VLIIGAGILGASAAYHLARLGAQVEIIDQNHPGKATL--AGAGVVCPWATEADDPDWYLL 60
Query: 64 MKRSVELWKKYSEEYN------FSFKQTGYLFLLYDEEEVETFK----RNIEIQNKFGVP 113
R + EE + + G L L D ++T + R I+ + G
Sbjct: 61 YARGARYYGTLIEELRGQGETELGYSRVGALVLAEDRARLDTIEGRISRRIKDAPEAGTV 120
Query: 114 TRLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLL-EYTEV 172
RL E + PL D E I P + D + A GA L +Y +
Sbjct: 121 RRLGAGEAKRLFPPLRDDLEAIHI---PGGARVDGRLLAASMLRVAISSGATLRNDYVSL 177
Query: 173 KGFIIENGEIKGVKTNRGTIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAV-ITQ 231
+ + +G + + ++ I ++ AWA I A+ G++ P+ P K Q + +
Sbjct: 178 R---LNDGRAECLGSDGRPIPADEIIVTAGAWAAQILALLGLRH--PVVPQKGQIIHLHL 232
Query: 232 PIKRGTINPMVISFKYGHAYLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKI 291
P + P+V+ Y+ ++ G E G +D T EV I
Sbjct: 233 PGVATSGWPVVLPMN--SYYMLAFDDSRVVVGATREDGSGFDYRVTARGQLEVLQAGLGI 290
Query: 292 IPALKNLLVLRTWAGYYAKTPDSNPAIGKIEELSDYYIAAGFSGHGFMMAPAVGEMIADL 351
P L + + T G+ P +G++ +++ I G G + P G ++A +
Sbjct: 291 APGLADATHIETRVGFRPAGSAMRPILGRVPQIAGLTIGNGLGASGLTVGPFAGHLLAGV 350
Query: 352 ITKGKSKLPVEWYDPYRFERG 372
+ +++P+E Y P E G
Sbjct: 351 VMGEPAEVPLERYSPTGPEAG 371
>emb|CAB53548.1| (AJ249196) D-alanine dehydrogenase [Rhizobium leguminosarum bv.
viciae]
Length = 416
Score = 73.0 bits (176), Expect = 6e-12
Identities = 68/281 (24%), Positives = 123/281 (43%), Gaps = 12/281 (4%)
Query: 83 KQTGYLFLLYDEEEVETFKRNIEIQNKFGVPTRLITPEEAKEIVPLLDI--SEVIAASWN 140
+ G L L ++++E ++++ G+P ++ + P L +++
Sbjct: 133 RMQGTLQLFRTQQQLEASAKDVKALAADGIPYEVLDRDGCIRFEPALKHVRDKIVGGLLT 192
Query: 141 PTDGKADPFEATTAFAIKAREYGAKLLEYTEVKGFIIENGEIKGVKTNRGTIKTGIVVNA 200
P D D F+ T A A KA G + T +K +E G ++GV T+R + VV A
Sbjct: 193 PKDETGDCFKFTNALAAKAEALGVRFAYGTTIKALDVEAGRVRGVITDRERMSAEAVVVA 252
Query: 201 TNAWAKLINAMAGIKVKIPIEPYKHQAVITQPIKRGTINP--MVISFKYGHAYLTQTFHG 258
+++ L+ GI ++P+ P K + +T PI + P V+ Y A +T+
Sbjct: 253 LGSYSPLLLKPLGI--RLPVYPVKGYS-LTIPIADASRAPESTVMDETYKIA-ITRLGDR 308
Query: 259 GIIGGIGYEIGPTYDLTPTYEFLREVSYYFTKIIPALKNLLVLRTWAGYYAKTPDSNPAI 318
+GG+ G T DL E Y T + P ++ W+G TPD P I
Sbjct: 309 IRVGGMAEISGYTNDLGLARRSTLE--YSVTDLFPG-GDISKASFWSGLRPMTPDGTPVI 365
Query: 319 GKIEELSDYYIAAGFSGHGFMMAPAVGEMIADLITKGKSKL 359
G +++ ++ G G+ M+ +I DL+ G+ ++
Sbjct: 366 GP-TKVAGLFLNTGHGTLGWTMSTGSARLIGDLVGGGQPEI 405
>emb|CAC11772.1| (AL445064) glycerol-3-phosphate dehydrogenase related protein
[Thermoplasma acidophilum]
Length = 422
Score = 69.5 bits (167), Expect = 6e-11
Identities = 91/373 (24%), Positives = 154/373 (40%), Gaps = 41/373 (10%)
Query: 1 MLPERSEVVVIGGGIVGVSIAHELAKRGVDVTVLEKRFIGSGSTFR----CGTGIRQQFN 56
M+ ++ VIG GI G+ A+ LA G DVTV E+ I SG++ R +G R N
Sbjct: 1 MMSMSEKIAVIGAGINGIFAAYYLAYAGYDVTVFERGDIDSGTSGRFHGMLHSGARYATN 60
Query: 57 DEANVRVM----KRSVELWKKYSEEYNFSFKQTGYLFLLYDEEEVETFKRNIEIQNKFGV 112
DE + R +R + +Y E TG FL +++E E + + G+
Sbjct: 61 DEVSARECIVENRRISNIGHRYVE-------NTGGYFLAANDDEAEFGDVLFDACRRAGI 113
Query: 113 PTRLITPEEAKEIVPLLDISEVIAASWNPTDGKADPFEATTAFAIKAREYGAKLLEYTEV 172
I EE P L + + D +E + A A G K+ Y++V
Sbjct: 114 DISEIPIEEFARDNPQLKNAR---RAMRVPDKVIRSYEFVVSVAAAAHLMGTKIRTYSDV 170
Query: 173 KGFIIENGEIKGVKTNRG----TIKTGIVVNATNAWAKLINAMAGIKVKIPIEPYKHQAV 228
K IE + G++ R + K VVNAT ++ I +G+K + P+ P V
Sbjct: 171 KKIDIEGNSVTGIEYERSGKEISEKFDAVVNATGPFSGKILERSGLK-QTPVMPSAGIMV 229
Query: 229 ITQPIKRGTINPMVISFKYGHA---YLTQTFHGGIIGGIGYEIGPTYDLTPTYEFLREVS 285
+ + G ++ +++ + L + I+G + P E ++ +
Sbjct: 230 VLE----GRVSTAILNRMREPSDADILLPYWSNSILGTSAVVVEDPDSFRPEPEDMQMMI 285
Query: 286 YYFTKIIPALKNLLVLRTW---------AGYYAKTPDSNPAIGKIEELSDYYIAAGFSGH 336
++++PA+K + + R + AG A+T + I E+L D ++ G
Sbjct: 286 DDMSELVPAVKIMPIKRYYYSVRPLIEDAGEDARTASRSFRIVGEEDLDDTLLSV--VGG 343
Query: 337 GFMMAPAVGEMIA 349
F VGE IA
Sbjct: 344 KFTTGRLVGEEIA 356
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.139 0.406
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 148384517
Number of Sequences: 2977
Number of extensions: 6461764
Number of successful extensions: 17084
Number of sequences better than 1.0e-10: 59
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 53
Number of HSP's that attempted gapping in prelim test: 16905
Number of HSP's gapped (non-prelim): 76
length of query: 382
length of database: 189,106,746
effective HSP length: 55
effective length of query: 327
effective length of database: 156,189,961
effective search space: 51074117247
effective search space used: 51074117247
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)