BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1854 (aif5A) DE:POSSIBLE INITIATION FACTOR 5A (aif5A)
(138 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||H75120 translation initiation factor aIF-5A PAB1854 [simila... 276 7e-74
sp|O50089|IF5A_PYRHO INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONT... 272 1e-72
sp|O26955|IF5A_METTH INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONT... 158 2e-38
gi|11498253 translation initiation factor eIF-5A (eif5A) [Archae... 145 2e-34
emb|CAC12187.1| (AL445066) translation initiation factor eIF-5a ... 143 1e-33
sp|P56635|IF5A_PYRAE INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONT... 138 3e-32
pdb|1BKB| Initiation Factor 5a From Archebacterium Pyrobaculum... 132 1e-30
sp|Q58625|IF5A_METJA INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONT... 131 5e-30
pdb|1EIF| Eukaryotic Translation Initiation Factor 5a From Met... 131 5e-30
pir||C64453 translation initiation factor aIF-5A MJ1228 [validat... 131 5e-30
pdb|2EIF|A Chain A, Eukaryotic Translation Initiation Factor 5a ... 131 5e-30
gb|AAK41244.1| Initiation factor 5A, hypothetical (eiF5A) [Sulfo... 127 4e-29
sp|P28461|IF5A_SULAC INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONT... 125 2e-28
sp|Q9YA53|IF5A_AERPE INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONT... 123 6e-28
gb|AAG19992.1| (AE005081) translation initiation factor eIF-5A; ... 103 1e-21
sp|P56335|IF53_SOLTU INITIATION FACTOR 5A-3 (EIF-5A) (EIF-4D) >g... 73 2e-12
sp|P13651|IF5A_DICDI INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|... 72 3e-12
sp|P56336|IF54_SOLTU INITIATION FACTOR 5A-4 (EIF-5A) (EIF-4D) >g... 70 1e-11
emb|CAB95733.1| (AJ278539) eukaryotic initiation factor 5a [Leis... 70 1e-11
emb|CAB65463.1| (AJ238624) translation initiation factor 5A prec... 69 2e-11
emb|CAA69990.1| (Y08735) orf-1 [Acidianus ambivalens] 69 3e-11
sp|P56332|IF5A_MAIZE INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|... 68 4e-11
sp|P56333|IF51_SOLTU INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|... 68 4e-11
gb|AAF27938.1|AF225297_1 (AF225297) translation initiation facto... 68 4e-11
emb|CAB96075.1| (AJ252135) translation initiation factor, eIF-5A... 68 4e-11
sp|P24922|IF52_NICPL INITIATION FACTOR 5A-2 (EIF-5A) (EIF-4D) >g... 68 4e-11
gb|AAF87023.1|AC006535_1 (AC006535) T24P13.1 [Arabidopsis thaliana] 68 4e-11
gb|AAC67555.1| (AF094773) translation initiation factor 5A [Oryz... 68 5e-11
sp|P26564|IF5A_MEDSA INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|... 67 8e-11
sp|P56337|IF55_SOLTU INITIATION FACTOR 5A-5 (EIF-5A) (EIF-4D) >g... 67 8e-11
sp|Q20751|IF52_CAEEL PROBABLE INITIATION FACTOR 5A (EIF-5A) (EIF... 67 8e-11
>pir||H75120 translation initiation factor aIF-5A PAB1854 [similarity] -
Pyrococcus abyssi (strain Orsay)
>gi|5458192|emb|CAB49681.1| (AJ248285) POSSIBLE
INITIATION FACTOR 5A (aif5A) [Pyrococcus abyssi]
Length = 138
Score = 276 bits (699), Expect = 7e-74
Identities = 138/138 (100%), Positives = 138/138 (100%)
Query: 1 MGDKTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVK 60
MGDKTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVK
Sbjct: 1 MGDKTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVK 60
Query: 61 PTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVE 120
PTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVE
Sbjct: 61 PTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVE 120
Query: 121 YWETLGRVKIMRIKGEGE 138
YWETLGRVKIMRIKGEGE
Sbjct: 121 YWETLGRVKIMRIKGEGE 138
>sp|O50089|IF5A_PYRHO INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONTAINING PROTEIN)
>gi|7443513|pir||G71010 translation initiation factor
aIF-5A PH1381 [similarity] - Pyrococcus horikoshii
>gi|3257804|dbj|BAA30487.1| (AP000006) 138aa long
hypothetical translation initiation factor eIF-5a
[Pyrococcus horikoshii]
Length = 138
Score = 272 bits (689), Expect = 1e-72
Identities = 135/138 (97%), Positives = 137/138 (98%)
Query: 1 MGDKTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVK 60
MGDKTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVK
Sbjct: 1 MGDKTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVK 60
Query: 61 PTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVE 120
PTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYE FEVPIDTGVA+EIRDQLKEGINVE
Sbjct: 61 PTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYETFEVPIDTGVADEIRDQLKEGINVE 120
Query: 121 YWETLGRVKIMRIKGEGE 138
YWETLGR+KIMRIKGEGE
Sbjct: 121 YWETLGRIKIMRIKGEGE 138
>sp|O26955|IF5A_METTH INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONTAINING PROTEIN)
>gi|7443515|pir||A69216 translation initiation factor
aIF-5A MTH869 [similarity] - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621962|gb|AAB85367.1| (AE000863) translation
initiation factor, eIF-5A [Methanobacterium
thermoautotrophicum]
Length = 130
Score = 158 bits (396), Expect = 2e-38
Identities = 80/132 (60%), Positives = 99/132 (74%), Gaps = 4/132 (3%)
Query: 4 KTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTS 63
K V+V LK G+Y+IID E +I NI+ SSPGKHGSAKAR+EAVGIFD + RS VKP
Sbjct: 3 KKVVEVKTLKVGKYVIIDGEASKITNISTSSPGKHGSAKARVEAVGIFDNQKRSFVKPVD 62
Query: 64 AEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWE 123
++VD+PIIDK+TAQVIAI VQ+MD+ETYE FE PI +E+ +QL EG+ VEY E
Sbjct: 63 SKVDIPIIDKRTAQVIAIMGGDVQLMDLETYETFETPI----PDELSEQLVEGVEVEYIE 118
Query: 124 TLGRVKIMRIKG 135
LG+ K+MR KG
Sbjct: 119 ALGQRKLMRTKG 130
>gi|11498253 translation initiation factor eIF-5A (eif5A) [Archaeoglobus
fulgidus] >gi|3334234|sp|O29612|IF5A_ARCFU INITIATION
FACTOR 5A (IF-5A) (HYPUSINE-CONTAINING PROTEIN)
>gi|7443514|pir||E69330 translation initiation factor
aIF-5A AF0645 [similarity] - Archaeoglobus fulgidus
>gi|2649972|gb|AAB90593.1| (AE001060) translation
initiation factor eIF-5A (eif5A) [Archaeoglobus
fulgidus]
Length = 128
Score = 145 bits (362), Expect = 2e-34
Identities = 67/130 (51%), Positives = 103/130 (78%), Gaps = 4/130 (3%)
Query: 4 KTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTS 63
K +V+V +L+ G Y++IDDEPC I++I+VS PGKHG+AKARI+A+GIFD + RSIV+P +
Sbjct: 2 KQQVEVRQLREGGYVVIDDEPCEILSISVSKPGKHGAAKARIDAIGIFDSQKRSIVQPVT 61
Query: 64 AEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWE 123
A++ VPI+++K AQ+I++T + Q+MD+ETYE FE+ V EE++D++++G V Y E
Sbjct: 62 AKIYVPIVERKRAQIISVTGNVAQLMDLETYETFELE----VPEELKDKMEQGREVIYLE 117
Query: 124 TLGRVKIMRI 133
+LG+ KI R+
Sbjct: 118 SLGKRKIERM 127
>emb|CAC12187.1| (AL445066) translation initiation factor eIF-5a related protein
[Thermoplasma acidophilum]
Length = 129
Score = 143 bits (356), Expect = 1e-33
Identities = 67/126 (53%), Positives = 92/126 (72%), Gaps = 4/126 (3%)
Query: 6 KVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAE 65
+ +V +LK GRYI+IDD PC+IV IT+S PGKHG AK RI A+G+FD + RS+V P +
Sbjct: 5 EAEVRELKVGRYILIDDAPCKIVEITMSKPGKHGEAKGRIVAIGVFDNQKRSVVYPVKHK 64
Query: 66 VDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWETL 125
V +PII+KK AQV+++ + VQ+MD ET+E F +P+D EI D++K G+ V YWET+
Sbjct: 65 VKIPIIEKKNAQVLSVQNNEVQLMDSETFETFVLPLD----PEIADKIKPGMEVPYWETM 120
Query: 126 GRVKIM 131
G KIM
Sbjct: 121 GMRKIM 126
>sp|P56635|IF5A_PYRAE INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONTAINING PROTEIN)
Length = 136
Score = 138 bits (344), Expect = 3e-32
Identities = 66/129 (51%), Positives = 91/129 (70%), Gaps = 1/129 (0%)
Query: 7 VQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEV 66
V+ +LK G Y++ID EPCR+V I S GKHGSAKARI AVG+FDG R++ P A+V
Sbjct: 9 VEAGELKEGSYVVIDGEPCRVVEIEKSKTGKHGSAKARIVAVGVFDGGKRTLSLPVDAQV 68
Query: 67 DVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWETLG 126
+VPII+K TAQ+++++ D +Q+MDM Y+ EVP+ V EE + +L G VE W+ L
Sbjct: 69 EVPIIEKFTAQILSVSGDVIQLMDMRDYKTIEVPMKY-VEEEAKGRLAPGAEVEVWQILD 127
Query: 127 RVKIMRIKG 135
R KI+R+KG
Sbjct: 128 RYKIIRVKG 136
>pdb|1BKB| Initiation Factor 5a From Archebacterium Pyrobaculum Aerophilum
Length = 136
Score = 132 bits (330), Expect = 1e-30
Identities = 64/129 (49%), Positives = 88/129 (67%), Gaps = 1/129 (0%)
Query: 7 VQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEV 66
V+ +LK G Y++ID EPCR+V I S GKHGSAKARI AVG+FDG R++ P A+V
Sbjct: 9 VEAGELKEGSYVVIDGEPCRVVEIEKSKTGKHGSAKARIVAVGVFDGGKRTLSLPVDAQV 68
Query: 67 DVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWETLG 126
+VPII+K TAQ+++++ D +Q+ D Y+ EVP V EE + +L G VE W+ L
Sbjct: 69 EVPIIEKFTAQILSVSGDVIQLXDXRDYKTIEVPXKY-VEEEAKGRLAPGAEVEVWQILD 127
Query: 127 RVKIMRIKG 135
R KI+R+KG
Sbjct: 128 RYKIIRVKG 136
>sp|Q58625|IF5A_METJA INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONTAINING PROTEIN)
>gi|2826380|gb|AAB99231.1| (U67563) translation
initiation factor aIF-5A [Methanococcus jannaschii]
Length = 132
Score = 131 bits (325), Expect = 5e-30
Identities = 65/130 (50%), Positives = 90/130 (69%), Gaps = 5/130 (3%)
Query: 6 KVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAE 65
+V V LK G+Y++ID PC IV+I+VS PGKHG AKAR+ +GIF+ + V PTS++
Sbjct: 6 QVNVGSLKVGQYVMIDGVPCEIVDISVSKPGKHGGAKARVVGIGIFEKVKKEFVAPTSSK 65
Query: 66 VDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWETL 125
V+VPIID++ QV+AI D VQIMD++TYE E+PI G+ + L+ G VEY E +
Sbjct: 66 VEVPIIDRRKGQVLAIMGDMVQIMDLQTYETLELPIPEGI-----EGLEPGGEVEYIEAV 120
Query: 126 GRVKIMRIKG 135
G+ KI R+ G
Sbjct: 121 GQYKITRVIG 130
>pdb|1EIF| Eukaryotic Translation Initiation Factor 5a From Methanococcus
Jannaschii
Length = 135
Score = 131 bits (325), Expect = 5e-30
Identities = 65/130 (50%), Positives = 90/130 (69%), Gaps = 5/130 (3%)
Query: 6 KVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAE 65
+V V LK G+Y++ID PC IV+I+VS PGKHG AKAR+ +GIF+ + V PTS++
Sbjct: 9 QVNVGSLKVGQYVMIDGVPCEIVDISVSKPGKHGGAKARVVGIGIFEKVKKEFVAPTSSK 68
Query: 66 VDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWETL 125
V+VPIID++ QV+AI D VQIMD++TYE E+PI G+ + L+ G VEY E +
Sbjct: 69 VEVPIIDRRKGQVLAIMGDMVQIMDLQTYETLELPIPEGI-----EGLEPGGEVEYIEAV 123
Query: 126 GRVKIMRIKG 135
G+ KI R+ G
Sbjct: 124 GQYKITRVIG 133
>pir||C64453 translation initiation factor aIF-5A MJ1228 [validated] -
Methanococcus jannaschii
Length = 135
Score = 131 bits (325), Expect = 5e-30
Identities = 65/130 (50%), Positives = 90/130 (69%), Gaps = 5/130 (3%)
Query: 6 KVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAE 65
+V V LK G+Y++ID PC IV+I+VS PGKHG AKAR+ +GIF+ + V PTS++
Sbjct: 9 QVNVGSLKVGQYVMIDGVPCEIVDISVSKPGKHGGAKARVVGIGIFEKVKKEFVAPTSSK 68
Query: 66 VDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWETL 125
V+VPIID++ QV+AI D VQIMD++TYE E+PI G+ + L+ G VEY E +
Sbjct: 69 VEVPIIDRRKGQVLAIMGDMVQIMDLQTYETLELPIPEGI-----EGLEPGGEVEYIEAV 123
Query: 126 GRVKIMRIKG 135
G+ KI R+ G
Sbjct: 124 GQYKITRVIG 133
>pdb|2EIF|A Chain A, Eukaryotic Translation Initiation Factor 5a From
Methanococcus Jannaschii
Length = 136
Score = 131 bits (325), Expect = 5e-30
Identities = 65/130 (50%), Positives = 90/130 (69%), Gaps = 5/130 (3%)
Query: 6 KVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAE 65
+V V LK G+Y++ID PC IV+I+VS PGKHG AKAR+ +GIF+ + V PTS++
Sbjct: 10 QVNVGSLKVGQYVMIDGVPCEIVDISVSKPGKHGGAKARVVGIGIFEKVKKEFVAPTSSK 69
Query: 66 VDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWETL 125
V+VPIID++ QV+AI D VQIMD++TYE E+PI G+ + L+ G VEY E +
Sbjct: 70 VEVPIIDRRKGQVLAIMGDMVQIMDLQTYETLELPIPEGI-----EGLEPGGEVEYIEAV 124
Query: 126 GRVKIMRIKG 135
G+ KI R+ G
Sbjct: 125 GQYKITRVIG 134
>gb|AAK41244.1| Initiation factor 5A, hypothetical (eiF5A) [Sulfolobus
solfataricus]
Length = 131
Score = 127 bits (317), Expect = 4e-29
Identities = 57/130 (43%), Positives = 88/130 (66%), Gaps = 2/130 (1%)
Query: 5 TKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSA 64
T V +LK G Y++ID EPCR+V +T + GKHGSAKA + A+G+F G ++++ P
Sbjct: 4 TYTTVGELKVGSYVVIDGEPCRVVEVTKAKTGKHGSAKANVVAIGVFSGAKKTLMAPVDQ 63
Query: 65 EVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYWET 124
+V+VPII+K Q+IA + +Q+MD+E+YE FE I+ +E+ ++K +EYWE
Sbjct: 64 QVEVPIIEKHIGQIIADMGNKIQVMDLESYETFE--IEKPTEDELASKIKPNAELEYWEI 121
Query: 125 LGRVKIMRIK 134
+GR KI+R+K
Sbjct: 122 MGRRKIVRVK 131
>sp|P28461|IF5A_SULAC INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONTAINING PROTEIN) (SHP)
>gi|282685|pir||S22380 translation initiation factor
aIF-5A [similarity] - Sulfolobus acidocaldarius
>gi|46593|emb|CAA44842.1| (X63132) hypusine-containing
protein [Sulfolobus acidocaldarius]
Length = 135
Score = 125 bits (312), Expect = 2e-28
Identities = 60/137 (43%), Positives = 90/137 (64%), Gaps = 5/137 (3%)
Query: 1 MGDKTKVQ---VSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRS 57
MG + +Q V LK G Y++ID EPCR+V IT + GKHGSAKA + A+G+F G+ RS
Sbjct: 1 MGIQMSIQYTTVGDLKVGSYVMIDGEPCRVVEITKAKTGKHGSAKANVVAIGLFTGQKRS 60
Query: 58 IVKPTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGI 117
++ P +V+VPII+K Q++A D + IMD+E+YE F+ ++ EI +++ G
Sbjct: 61 LMAPVDQQVEVPIIEKHVGQILADKGDNLTIMDLESYETFD--LEKPTENEIVSKIRPGA 118
Query: 118 NVEYWETLGRVKIMRIK 134
+EYW +GR KI+R+K
Sbjct: 119 EIEYWSVMGRRKIVRVK 135
>sp|Q9YA53|IF5A_AERPE INITIATION FACTOR 5A (IF-5A) (HYPUSINE-CONTAINING PROTEIN)
>gi|7521153|pir||H72513 translation initiation factor
aIF-5a APE2085 [similarity] - Aeropyrum pernix (strain
K1) >gi|5105784|dbj|BAA81096.1| (AP000063) 148aa long
hypothetical initiation factor 5A [Aeropyrum pernix]
Length = 148
Score = 123 bits (307), Expect = 6e-28
Identities = 60/125 (48%), Positives = 85/125 (68%), Gaps = 2/125 (1%)
Query: 12 LKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVDVPII 71
LK G YI+ID EPCRIV ++ + GKHGSAKA + A+ +F G+ +S+V P V +P+I
Sbjct: 20 LKKGSYIVIDGEPCRIVEMSRAKTGKHGSAKAHVVAICVFSGQKKSLVAPVDTRVQIPVI 79
Query: 72 DKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEE--IRDQLKEGINVEYWETLGRVK 129
+K+ QV+A D VQIMD+ETY+ FEV G EE + +L+ G+ VEYW +G+ K
Sbjct: 80 EKRLGQVLADMGDMVQIMDLETYDTFEVEKPGGNEEEEQLAAKLQPGVTVEYWLIMGKPK 139
Query: 130 IMRIK 134
I+RI+
Sbjct: 140 IIRIR 144
>gb|AAG19992.1| (AE005081) translation initiation factor eIF-5A; Eif5a
[Halobacterium sp. NRC-1]
Length = 127
Score = 103 bits (254), Expect = 1e-21
Identities = 55/130 (42%), Positives = 85/130 (65%), Gaps = 7/130 (5%)
Query: 4 KTKVQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTS 63
K + +V L+ G Y++++D C+I + + PGKHGSAKARIEA G+FDGK RS+ +P
Sbjct: 3 KEQKEVRDLQEGNYVMMEDAACQINAYSTAKPGKHGSAKARIEAEGVFDGKKRSLSQPVD 62
Query: 64 AEVDVPIIDKKTAQVIAITPDTV-QIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVEYW 122
A++ VPI+++K Q+++ DTV Q+MD+ETYE + + E+ Q E N+EY
Sbjct: 63 AKIWVPIVNRKQGQIVSKESDTVAQVMDLETYE----TVTMQIPGELDIQADE--NIEYL 116
Query: 123 ETLGRVKIMR 132
E G+ KI++
Sbjct: 117 EFEGQRKILQ 126
>sp|P56335|IF53_SOLTU INITIATION FACTOR 5A-3 (EIF-5A) (EIF-4D) >gi|7443511|pir||T07133
translation initiation factor eIF-5A.3 [similarity] -
potato >gi|2225881|dbj|BAA20877.1| (AB004824) eukaryotic
initiation factor 5A3 [Solanum tuberosum]
Length = 160
Score = 72.6 bits (175), Expect = 2e-12
Identities = 41/135 (30%), Positives = 69/135 (50%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ YI+I PC++V ++ S GKHG AK A+ IF+GK + P+S D
Sbjct: 23 QAGTIRKNGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCD 82
Query: 68 VPIIDKKTAQVIAITPDTVQIMDME---TYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D + E T + +P D + ++++D +EG + V
Sbjct: 83 VPHVNRTDYQLIDISEDGFVSLLTESGNTKDDLRLPTDESLLKQVKDGFQEGKDLVVSVM 142
Query: 123 ETLGRVKIMRIKGEG 137
+G +I IK G
Sbjct: 143 SAMGEEQINAIKDIG 157
>sp|P13651|IF5A_DICDI INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|72891|pir||FIDOA
translation initiation factor eIF-5A [validated] - slime
mold (Dictyostelium discoideum) >gi|7279|emb|CAA33095.1|
(X14970) eIF-4D (AA 1-169) [Dictyostelium discoideum]
>gi|226313|prf||1506341A initiation factor eIF4D
[Dictyostelium discoideum]
Length = 169
Score = 71.8 bits (173), Expect = 3e-12
Identities = 42/132 (31%), Positives = 70/132 (52%), Gaps = 5/132 (3%)
Query: 7 VQVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEV 66
+Q S L+ +++I PC+IV+++ S GKHG AK I A+ IF GK + P++ +
Sbjct: 35 IQCSALRKNGFVVIKGFPCKIVDMSTSKTGKHGHAKVNITAIDIFTGKKYEEICPSTHNI 94
Query: 67 DVPIIDKKTAQVIAITPDTVQIMDM--ETYEMFEVPIDTGVAEEIRDQLKEGIN--VEYW 122
DVP + +K V+ + + ++D E E +P D + +EI LKEG V
Sbjct: 95 DVPNVSRKEYTVMDVQDGYLSLLDAGGEVKEDLALPED-DIGKEITQMLKEGKEPLVSVI 153
Query: 123 ETLGRVKIMRIK 134
LG+ ++ +K
Sbjct: 154 SALGKEGVVSVK 165
>sp|P56336|IF54_SOLTU INITIATION FACTOR 5A-4 (EIF-5A) (EIF-4D)
>gi|2225883|dbj|BAA20878.1| (AB004825) eukaryotic
initiation factor 5A4 [Solanum tuberosum]
Length = 159
Score = 69.9 bits (168), Expect = 1e-11
Identities = 40/135 (29%), Positives = 67/135 (49%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ YI+I PC++V ++ S GKHG AK A+ IF GK + P+S D
Sbjct: 23 QAGTIRKSGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCD 82
Query: 68 VPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D ++ + T + +P D + +I+D EG + V
Sbjct: 83 VPHVNRTDYQLIDISEDGFVSLLTDNGNTKDDLRLPTDDSLLSQIKDGFAEGKDLVVSVM 142
Query: 123 ETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 143 SAMGEEQINALKDIG 157
>emb|CAB95733.1| (AJ278539) eukaryotic initiation factor 5a [Leishmania infantum]
Length = 166
Score = 69.9 bits (168), Expect = 1e-11
Identities = 42/138 (30%), Positives = 71/138 (51%), Gaps = 11/138 (7%)
Query: 12 LKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVDVPII 71
LK G Y+ I+ PC++++++VS GKHG AK I A IF G P++ V+VP +
Sbjct: 28 LKKGGYVCINGRPCKVIDLSVSKTGKHGHAKVSIVATDIFTGNRLEDQAPSTHNVEVPFV 87
Query: 72 DKKTAQVIAITPDT-------VQIMD--METYEMFEVPIDTGVAEEIRDQLKEG--INVE 120
T V+ I P+ + +MD E+ E ++P D +A +I++Q G + V
Sbjct: 88 KTFTYSVLDIQPNEDPSLPSHLSLMDDEGESREDLDMPPDVALATQIKEQFDSGKEVLVV 147
Query: 121 YWETLGRVKIMRIKGEGE 138
+G ++++ K E
Sbjct: 148 VVSAMGTEQVLQTKNAAE 165
>emb|CAB65463.1| (AJ238624) translation initiation factor 5A precursor protein
(eIF-5A) [Senecio vernalis]
Length = 159
Score = 69.1 bits (166), Expect = 2e-11
Identities = 39/135 (28%), Positives = 70/135 (50%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ G +I+I + C++V ++ S GKHG AK A+ IF+GK + P+S D
Sbjct: 23 QAGTIRKGGHIVIKNRACKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCD 82
Query: 68 VPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D ++ + T + ++P D + +I+D EG + V
Sbjct: 83 VPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDALLTQIKDGFGEGKDLVVSVM 142
Query: 123 ETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 143 SAMGEEQICALKDIG 157
>emb|CAA69990.1| (Y08735) orf-1 [Acidianus ambivalens]
Length = 74
Score = 68.7 bits (165), Expect = 3e-11
Identities = 30/74 (40%), Positives = 49/74 (65%), Gaps = 2/74 (2%)
Query: 61 PTSAEVDVPIIDKKTAQVIAITPDTVQIMDMETYEMFEVPIDTGVAEEIRDQLKEGINVE 120
P +V++PII+K Q++A D +Q+MDM+TYE FE ++ E++ +++ G VE
Sbjct: 3 PVDDQVEIPIIEKHVGQILADMGDKIQVMDMDTYETFE--MEKPKEEDLASKIRPGAEVE 60
Query: 121 YWETLGRVKIMRIK 134
YW +GR KI+R+K
Sbjct: 61 YWTIMGRRKIVRVK 74
>sp|P56332|IF5A_MAIZE INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|7443512|pir||T01355
translation initiation factor eIF-5A [similarity] -
maize >gi|1546919|emb|CAA69225.1| (Y07920) translation
initiation factor 5A [Zea mays]
>gi|2668738|gb|AAB88614.1| (AF034943) translation
initiation factor 5A [Zea mays]
Length = 160
Score = 68.3 bits (164), Expect = 4e-11
Identities = 37/135 (27%), Positives = 69/135 (50%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ +I+I + PC++V ++ S GKHG AK A+ IF+GK + P+S D
Sbjct: 24 QAGTVRKNGFIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCD 83
Query: 68 VPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
+P +++ Q+I I+ D ++ D T + +P D + +I++ + G + V
Sbjct: 84 IPHVNRTEYQLIDISEDGFVSLLTSDGNTKDDLRLPTDETLVAQIKEGFESGKDLVVTVQ 143
Query: 123 ETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 144 SAMGEEQICALKDVG 158
>sp|P56333|IF51_SOLTU INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|2225879|dbj|BAA20876.1|
(AB004823) eukaryotic initiation factor 5A2 [Solanum
tuberosum] >gi|2225887|dbj|BAA20880.1| (AB004827)
eukaryotic initiation factor 5A1 [Solanum tuberosum]
Length = 160
Score = 68.3 bits (164), Expect = 4e-11
Identities = 37/135 (27%), Positives = 68/135 (49%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ YI+I PC++V ++ S GKHG AK A+ IF+GK + P+S D
Sbjct: 23 QAGTIRKSGYIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCD 82
Query: 68 VPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D ++ + T + +P D + +++ +EG + +
Sbjct: 83 VPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDALLNQVKGGFEEGKDLVLSVM 142
Query: 123 ETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 143 SAMGEEQICAVKDIG 157
>gb|AAF27938.1|AF225297_1 (AF225297) translation initiation factor 5A [Euphorbia esula]
Length = 156
Score = 68.3 bits (164), Expect = 4e-11
Identities = 38/135 (28%), Positives = 68/135 (50%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ YI+I + PC++V ++ S GKHG AK + IF+GK + P+S D
Sbjct: 20 QAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVGIDIFNGKKLEDIVPSSHNCD 79
Query: 68 VPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP + + Q+I I+ D ++ + T + +P D + +I+D +G + V
Sbjct: 80 VPHVTRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDESLLSQIKDGFGDGKDLVVTVM 139
Query: 123 ETLGRVKIMRIKGEG 137
++G +I +K G
Sbjct: 140 SSMGEEQICALKDIG 154
>emb|CAB96075.1| (AJ252135) translation initiation factor, eIF-5A [Oryza sativa]
Length = 161
Score = 68.3 bits (164), Expect = 4e-11
Identities = 40/135 (29%), Positives = 68/135 (49%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ +I+I + PC++V ++ S GKHG AK A+ IF+GK + P+S D
Sbjct: 24 QAGTIRKTGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCD 83
Query: 68 VPIIDKKTAQVIAITPDTVQIMDME---TYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D + E T + +P D + +I+D EG + V
Sbjct: 84 VPHVNRTDYQLIDISEDGFVSLLTESGGTKDDLRLPSDEALLTQIKDGFAEGKDLIVTVM 143
Query: 123 ETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 144 SAMGEEQICALKDIG 158
>sp|P24922|IF52_NICPL INITIATION FACTOR 5A-2 (EIF-5A) (EIF-4D) >gi|100278|pir||S21059
translation initiation factor eIF-5A.2 [similarity] -
curled-leaved tobacco >gi|19702|emb|CAA45104.1| (X63542)
eukaryotic initiation factor 5A (2) [Nicotiana
plumbaginifolia]
Length = 159
Score = 68.3 bits (164), Expect = 4e-11
Identities = 39/135 (28%), Positives = 67/135 (48%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ +I+I PC++V ++ S GKHG AK A+ IF GK + P+S D
Sbjct: 23 QAGTIRKNGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCD 82
Query: 68 VPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D ++ + T + +P D + +I+D EG + V
Sbjct: 83 VPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDNLLTQIKDGFAEGKDLVVSVM 142
Query: 123 ETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 143 SAMGEEQICALKDIG 157
>gb|AAF87023.1|AC006535_1 (AC006535) T24P13.1 [Arabidopsis thaliana]
Length = 216
Score = 68.3 bits (164), Expect = 4e-11
Identities = 40/131 (30%), Positives = 68/131 (51%), Gaps = 5/131 (3%)
Query: 12 LKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVDVPII 71
++ G +I+I + PC++V ++ S GKHG AK A+ IF K + P+S DVP +
Sbjct: 26 IRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTAKKLEDIVPSSHNCDVPHV 85
Query: 72 DKKTAQVIAITPDTVQIMDME---TYEMFEVPIDTGVAEEIRDQLKEG--INVEYWETLG 126
++ Q+I IT D + + T + ++P D G+ ++R EG I V ++G
Sbjct: 86 NRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRLGFDEGKDIVVSVMSSMG 145
Query: 127 RVKIMRIKGEG 137
+I +K G
Sbjct: 146 EEQICAVKEVG 156
>gb|AAC67555.1| (AF094773) translation initiation factor 5A [Oryza sativa]
Length = 160
Score = 67.9 bits (163), Expect = 5e-11
Identities = 40/135 (29%), Positives = 67/135 (49%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ YI+I + PC++V ++ S GKHG AK A+ IF+GK + P+S D
Sbjct: 24 QAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCD 83
Query: 68 VPIIDKKTAQVIAITPDTVQIMDME---TYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D + E T + +P D + +I+ EG + V
Sbjct: 84 VPHVNRTEYQLIDISEDGFVSLLTESGNTKDDLRLPTDDSLLGQIKTGFGEGKDLVVTVM 143
Query: 123 ETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 144 SAMGEEQICALKDIG 158
>sp|P26564|IF5A_MEDSA INITIATION FACTOR 5A (EIF-5A) (EIF-4D) >gi|72894|pir||FIAAA
translation initiation factor eIF-5A [similarity] -
alfalfa >gi|19601|emb|CAA42065.1| (X59441) eukaryotic
translation initiation factor 4D [Medicago sativa]
Length = 161
Score = 67.1 bits (161), Expect = 8e-11
Identities = 39/136 (28%), Positives = 69/136 (50%), Gaps = 6/136 (4%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDG-KVRSIVKPTSAEV 66
Q ++ YI+I + PC++V ++ S GKHG AK A+ IF K+ + P+S
Sbjct: 23 QAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTSKKLEEVYVPSSHNC 82
Query: 67 DVPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEY 121
DVP +++ Q+I I+ D ++ + T + ++P D + +I+D EG + V
Sbjct: 83 DVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDSLLTQIKDGFAEGKDLVVSV 142
Query: 122 WETLGRVKIMRIKGEG 137
+G +I +K G
Sbjct: 143 MSAMGEEQICALKDIG 158
>sp|P56337|IF55_SOLTU INITIATION FACTOR 5A-5 (EIF-5A) (EIF-4D)
>gi|2225885|dbj|BAA20879.1| (AB004826) eukaryotic
initiation factor 5A5 [Solanum tuberosum]
Length = 159
Score = 67.1 bits (161), Expect = 8e-11
Identities = 38/135 (28%), Positives = 68/135 (50%), Gaps = 5/135 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q ++ +I+I + PC++V ++ S GKHG AK A+ IF GK + P+S D
Sbjct: 23 QAGTIRKSGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTGKKLEDIVPSSHNCD 82
Query: 68 VPIIDKKTAQVIAITPD---TVQIMDMETYEMFEVPIDTGVAEEIRDQLKEG--INVEYW 122
VP +++ Q+I I+ D ++ + T + +P D + +++D EG + +
Sbjct: 83 VPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTDDTLLAQVKDGFAEGKDLVLSVM 142
Query: 123 ETLGRVKIMRIKGEG 137
+G +I IK G
Sbjct: 143 SAMGEEQICGIKDIG 157
>sp|Q20751|IF52_CAEEL PROBABLE INITIATION FACTOR 5A (EIF-5A) (EIF-4D)
>gi|7504148|pir||T22628 translation initiation factor
eIF-5A F54C9.1 [similarity] - Caenorhabditis elegans
>gi|3877497|emb|CAA90247.1| (Z49967) contains similarity
to Pfam domain: PF01287 (Eukaryotic initiation factor 5A
hypusine (eIF-5A)), Score=348.5, E-value=2.4e-101,
N=1~cDNA EST yk112c12.5 comes from this gene; cDNA EST
yk74e6.5 comes from this gene~cDNA EST yk83d2.5 comes
from t>
Length = 161
Score = 67.1 bits (161), Expect = 8e-11
Identities = 35/107 (32%), Positives = 61/107 (56%), Gaps = 4/107 (3%)
Query: 8 QVSKLKPGRYIIIDDEPCRIVNITVSSPGKHGSAKARIEAVGIFDGKVRSIVKPTSAEVD 67
Q S L+ +++I PC+IV ++ S GKHG AK + A+ IF K + P++ +D
Sbjct: 25 QCSALRKNEHVMIKGRPCKIVEMSTSKTGKHGHAKVHMVAIDIFTSKKLEDICPSTHNMD 84
Query: 68 VPIIDKKTAQVIAITPDTVQIMDMETYEM---FEVPIDTGVAEEIRD 111
VP++ ++ ++AI +MD E+ E ++P DT + ++IRD
Sbjct: 85 VPVVKRREYLLMAIDDGYCSLMDPESCEQKDDLKLP-DTELGQQIRD 130
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.316 0.137 0.381
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47543936
Number of Sequences: 2977
Number of extensions: 1662182
Number of successful extensions: 3091
Number of sequences better than 1.0e-10: 31
Number of HSP's better than 0.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 3065
Number of HSP's gapped (non-prelim): 31
length of query: 138
length of database: 189,106,746
effective HSP length: 55
effective length of query: 83
effective length of database: 156,189,961
effective search space: 12963766763
effective search space used: 12963766763
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 42 (22.0 bits)
S2: 161 (67.1 bits)