BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1869 (thrC-2) DE:threonine synthase (thrC-2)
(439 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75118 probable threonine synthase (EC 4.2.99.2) thrc-2 PAB... 878 0.0
pir||H71013 probable threonine synthase (EC 4.2.99.2) PH1406 [si... 778 0.0
sp|Q58860|THRC_METJA PROBABLE THREONINE SYNTHASE >gi|2118306|pir... 249 6e-65
pir||G75080 threonine synthase (EC 4.2.99.2) thrc-2 PAB1677 [sim... 245 7e-64
pir||E71136 threonine synthase (EC 4.2.99.2) PH0857 [similarity]... 239 5e-62
gb|AAK41164.1| Threonine synthase (thrC-2) [Sulfolobus solfatari... 233 4e-60
pir||G69131 threonine synthase (EC 4.2.99.2) - Methanobacterium ... 224 2e-57
pir||B72455 threonine synthase (EC 4.2.99.2) APE2286 [similarity... 210 3e-53
gi|11498914 threonine synthase (thrC-2) [Archaeoglobus fulgidus]... 208 8e-53
gi|11498161 threonine synthase (thrC-1) [Archaeoglobus fulgidus]... 198 9e-50
pir||D70354 threonine synthase (EC 4.2.99.2) thrC1 [similarity] ... 193 4e-48
gb|AAG20514.1| (AE005123) threonine synthase; ThrC1 [Halobacteri... 184 2e-45
sp|Q10610|THRC_MYCTU PROBABLE THREONINE SYNTHASE >gi|7436919|pir... 183 4e-45
pir||G72364 threonine synthase (EC 4.2.99.2) TM0546 [similarity]... 181 2e-44
sp|P45837|THRC_MYCLE PROBABLE THREONINE SYNTHASE >gi|7436934|pir... 177 2e-43
sp|P04990|THRC_BACSU THREONINE SYNTHASE >gi|80429|pir||A25364 th... 174 2e-42
pir||H70338 threonine synthase (EC 4.2.99.2) - Aquifex aeolicus ... 173 5e-42
emb|CAB93053.1| (AL357432) putative threonine synthase [Streptom... 171 1e-41
sp|P09123|THRC_BRELA THREONINE SYNTHASE >gi|68321|pir||DWFKTG th... 168 9e-41
gb|AAK40813.1| Threonine synthase (thrC-1) [Sulfolobus solfatari... 162 9e-39
pir||S77200 threonine synthase (EC 4.2.99.2) 1 - Synechocystis s... 157 3e-37
dbj|BAB07140.1| (AP001518) threonine synthase [Bacillus halodurans] 155 1e-36
pir||E75172 probable threonine synthase (EC 4.2.99.2) thrc-2 PAB... 152 6e-36
pir||C71042 probable threonine synthase (EC 4.2.99.2) PH1627 [si... 149 5e-35
sp|P74193|THRC_SYNY3 THREONINE SYNTHASE >gi|7436917|pir||S75823 ... 146 4e-34
gb|AAG19064.1| (AE005005) threonine synthase; ThrC2 [Halobacteri... 145 8e-34
emb|CAC11637.1| (AL445064) threonine synthase related protein [T... 129 5e-29
gb|AAD55628.1|AC008017_1 (AC008017) Putative threonine synthase ... 128 1e-28
gb|AAF74984.1|AF082894_1 (AF082894) threonine synthase [Solanum ... 127 2e-28
pir||S71362 threonine synthase (EC 4.2.99.2) precursor - Arabido... 127 3e-28
pir||T08545 threonine synthase (EC 4.2.99.2) precursor - Arabido... 127 3e-28
gb|AAG20250.1| (AE005100) threonine synthase; ThrC3 [Halobacteri... 125 9e-28
emb|CAC11175.1| (AL445063) threonine synthase related protein [T... 122 6e-27
pir||H72628 probable threonine synthase (EC 4.2.99.2) APE1488 [s... 109 6e-23
gb|AAB40316.1| (U46200) threonine synthase [Cloning vector pDG16... 108 2e-22
gb|AAK40587.1| Threonine dehydratase catabolic (threonine deamin... 87 3e-16
sp|P46493|THD1_HAEIN THREONINE DEHYDRATASE BIOSYNTHETIC (THREONI... 84 3e-15
pir||D72386 threonine dehydratase (EC 4.2.1.16) TM0356 [similari... 81 3e-14
sp|P05792|THD2_ECOLI THREONINE DEHYDRATASE CATABOLIC (THREONINE ... 79 9e-14
gb|AAF33479.1| (AF233324) S. typhimurium threonine deaminase (IL... 76 7e-13
gb|AAA24014.1| (K03503) threonine dehydratase [Escherichia coli] 75 1e-12
gb|AAA67575.1| (M87049) threonine deaminase [Escherichia coli] 75 1e-12
gb|AAF54364.1| (AE003682) CG8129 gene product [alt 1] [Drosophil... 75 1e-12
pir||T23166 hypothetical protein K01C8.1 - Caenorhabditis elegan... 75 2e-12
sp|P04968|THD1_ECOLI THREONINE DEHYDRATASE BIOSYNTHETIC (THREONI... 74 3e-12
sp|P20506|THD1_SALTY THREONINE DEHYDRATASE BIOSYNTHETIC (THREONI... 74 4e-12
gb|AAA24024.1| (M32253) ilvA [Escherichia coli] 74 4e-12
gb|AAG20244.1| (AE005099) threonine dehydratase; IluA [Halobacte... 72 1e-11
gb|AAF59508.1| (AC024805) contains similarity to SW:THD2_ECOLI [... 71 2e-11
sp|P25306|THD1_LYCES THREONINE DEHYDRATASE BIOSYNTHETIC PRECURSO... 71 2e-11
gb|AAA34171.1| (M61914) threonine deaminase [Lycopersicon escule... 71 2e-11
gb|AAB40319.1| (U46200) threonine synthase [Cloning vector pDG16... 71 3e-11
pir||D81355 threonine dehydratase (EC 4.2.1.16) Cj0828c [importe... 71 3e-11
>pir||E75118 probable threonine synthase (EC 4.2.99.2) thrc-2 PAB1869
[similarity] - Pyrococcus abyssi (strain Orsay)
>gi|5458173|emb|CAB49662.1| (AJ248285) threonine
synthase (thrC-2) [Pyrococcus abyssi]
Length = 439
Score = 878 bits (2245), Expect = 0.0
Identities = 439/439 (100%), Positives = 439/439 (100%)
Query: 1 MKCPKCGREYKAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIVS 60
MKCPKCGREYKAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIVS
Sbjct: 1 MKCPKCGREYKAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIVS 60
Query: 61 LQEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDG 120
LQEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDG
Sbjct: 61 LQEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDG 120
Query: 121 NAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKL 180
NAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKL
Sbjct: 121 NAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKL 180
Query: 181 NGLYNVTPEENIVGLEGQKTIAFELWEEINPTHVIVPTGSGSYLYSIYKGFKELIEIGAL 240
NGLYNVTPEENIVGLEGQKTIAFELWEEINPTHVIVPTGSGSYLYSIYKGFKELIEIGAL
Sbjct: 181 NGLYNVTPEENIVGLEGQKTIAFELWEEINPTHVIVPTGSGSYLYSIYKGFKELIEIGAL 240
Query: 241 EEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVL 300
EEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVL
Sbjct: 241 EEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVL 300
Query: 301 VSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLKTGEG 360
VSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLKTGEG
Sbjct: 301 VSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLKTGEG 360
Query: 361 GREKFGIGGTKLEILKILKDGEKYAYEIWQALGKPVKYQAIHQHIKELQELGLIEEAYRR 420
GREKFGIGGTKLEILKILKDGEKYAYEIWQALGKPVKYQAIHQHIKELQELGLIEEAYRR
Sbjct: 361 GREKFGIGGTKLEILKILKDGEKYAYEIWQALGKPVKYQAIHQHIKELQELGLIEEAYRR 420
Query: 421 GKRIYYKLTEKGIRLVDNL 439
GKRIYYKLTEKGIRLVDNL
Sbjct: 421 GKRIYYKLTEKGIRLVDNL 439
>pir||H71013 probable threonine synthase (EC 4.2.99.2) PH1406 [similarity] -
Pyrococcus horikoshii >gi|3257829|dbj|BAA30512.1|
(AP000006) 440aa long hypothetical threonine synthase
[Pyrococcus horikoshii]
Length = 440
Score = 778 bits (1987), Expect = 0.0
Identities = 375/439 (85%), Positives = 416/439 (94%)
Query: 1 MKCPKCGREYKAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIVS 60
MKCP+CGREY + IPPFCICGEELEITYDYSSVD RKWK R PGVWRYKELLP+VKKI++
Sbjct: 1 MKCPRCGREYISSIPPFCICGEELEITYDYSSVDVRKWKRRNPGVWRYKELLPEVKKIIT 60
Query: 61 LQEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDG 120
LQEGGTPLIKARISE++NVDV+IKDETRNPTGSFRDRLATVAISYGLPYA NGFIVASDG
Sbjct: 61 LQEGGTPLIKARISEKINVDVYIKDETRNPTGSFRDRLATVAISYGLPYASNGFIVASDG 120
Query: 121 NAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKL 180
NAAASVAAYSARAEKECFVV+PRKVDKGKLIQMIAFGARIIRYG+SVD+AIEYA+EL+KL
Sbjct: 121 NAAASVAAYSARAEKECFVVVPRKVDKGKLIQMIAFGARIIRYGDSVDEAIEYARELSKL 180
Query: 181 NGLYNVTPEENIVGLEGQKTIAFELWEEINPTHVIVPTGSGSYLYSIYKGFKELIEIGAL 240
NGLYNVTPE+NI+GLEGQKTIAFELWEEINPTHVIVPTGSGSY+YSIYKGFKELI+IG L
Sbjct: 181 NGLYNVTPEDNIIGLEGQKTIAFELWEEINPTHVIVPTGSGSYIYSIYKGFKELIKIGVL 240
Query: 241 EEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVL 300
E+MP+LIAVQT+RCNPIA+EILG+K ECKETKALGLYV+ P+MK +VIRAI+ES GTAV+
Sbjct: 241 EKMPRLIAVQTERCNPIAAEILGLKKECKETKALGLYVKNPVMKSRVIRAIKESGGTAVV 300
Query: 301 VSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLKTGEG 360
V+E +I GE+LLANEGIFAELSSAVVMPAL+ LV G IEKGD+VVLVVTGSGLK GEG
Sbjct: 301 VNENDIITGEKLLANEGIFAELSSAVVMPALLSLVEKGIIEKGDKVVLVVTGSGLKIGEG 360
Query: 361 GREKFGIGGTKLEILKILKDGEKYAYEIWQALGKPVKYQAIHQHIKELQELGLIEEAYRR 420
GREKF IGGTKLEILKIL++ E YAYEIWQALGKP+KYQA+HQHIKELQELGLI+EAYR+
Sbjct: 361 GREKFSIGGTKLEILKILEEREMYAYEIWQALGKPLKYQAVHQHIKELQELGLIKEAYRK 420
Query: 421 GKRIYYKLTEKGIRLVDNL 439
GKRIYYKLTE+G RL++NL
Sbjct: 421 GKRIYYKLTERGRRLIENL 439
>sp|Q58860|THRC_METJA PROBABLE THREONINE SYNTHASE >gi|2118306|pir||H64482 threonine
synthase (EC 4.2.99.2) - Methanococcus jannaschii
>gi|1592106|gb|AAB99473.1| (U67587) threonine synthase
(thrC) [Methanococcus jannaschii]
Length = 405
Score = 249 bits (628), Expect = 6e-65
Identities = 155/376 (41%), Positives = 223/376 (59%), Gaps = 24/376 (6%)
Query: 2 KCPKCGREYKA-EIPPFCICGEELEITYDYSSVDTR----KWKNRRPGVWRYKELLP--K 54
+C KCG+ Y EI C CG LEI YDY + + K + R GVWRY E LP
Sbjct: 4 RCIKCGKTYDVDEIIYTCECGGLLEIIYDYEEIKDKVSEEKLRKREIGVWRYLEYLPVKD 63
Query: 55 VKKIVSLQEGGTPLIKAR-ISEQLNV-DVFIKDETRNPTGSFRDRLATVAISYGLPYAKN 112
KIVSL EGGTPL + + ++L + ++++K+E NPTGSF+DR TV ++
Sbjct: 64 ESKIVSLCEGGTPLYRCNNLEKELGIKELYVKNEGANPTGSFKDRGMTVGVTRANELGVE 123
Query: 113 GFIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAI 171
AS GN +AS+AAYSAR+ K+C V++P KV GKL Q + +GA++I+ + DDA+
Sbjct: 124 VVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVIQVKGNFDDAL 183
Query: 172 EYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSIY 228
+ K+LAK +Y + N LEGQKTIAFE+ +++N P VIVP G+ + +I+
Sbjct: 184 DMVKQLAKEKLIYLLN-SINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVGNAGNISAIW 242
Query: 229 KGFKELIEIGALEEMPKLIAVQTDRCNPI-------ASEILGIKGECKETKALGLYVREP 281
KGFKE G ++E+PK+ +Q D PI A +I+ K ET A + + P
Sbjct: 243 KGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNP--ETIATAIRIGNP 300
Query: 282 LMKPKVIRAIRESSGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVMPALIKLVNDGFI 340
+ PK + AI S G A V++EEI + ++LLA EGIF E +SA + L KL+ +G I
Sbjct: 301 VNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKKLLEEGII 360
Query: 341 EKGDRVVLVVTGSGLK 356
++ +R+V + TG GLK
Sbjct: 361 DRDERIVCITTGHGLK 376
>pir||G75080 threonine synthase (EC 4.2.99.2) thrc-2 PAB1677 [similarity] -
Pyrococcus abyssi (strain Orsay)
>gi|5458460|emb|CAB49948.1| (AJ248286) threonine
synthase (thrC-2) [Pyrococcus abyssi]
Length = 394
Score = 245 bits (619), Expect = 7e-64
Identities = 147/367 (40%), Positives = 223/367 (60%), Gaps = 13/367 (3%)
Query: 1 MKCPKCGREY-KAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIV 59
++C +CGREY + EI C CG LE+ D VDT+ + +W+Y+ LP VKKIV
Sbjct: 3 LRCIRCGREYPEDEIRYRCECGGLLEVVVDLDKVDTKIFDGNNITLWKYESWLP-VKKIV 61
Query: 60 SLQEGGTPLIKARISEQLN--VDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVA 117
SL EGGTPL + E++ +++ K+E NPTGSF+DR TV +S L A
Sbjct: 62 SLNEGGTPLYRLNNLEKILGVKELYAKNEGANPTGSFKDRGMTVGVSKALELGMKRVACA 121
Query: 118 SDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYAKE 176
S GN +AS+AAY+A+A E +V++P K+ GKL Q I +GA++I + DDA+ E
Sbjct: 122 STGNTSASLAAYAAKAGIEAYVLVPSGKIALGKLAQAIIYGAKVIPIKGNFDDALRVVIE 181
Query: 177 LAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN--PTHVIVPTGSGSYLYSIYKGFKEL 234
++ G+Y + N LEGQKTIA+E+++++ P +V++P G+ + +I+KGFKEL
Sbjct: 182 ASEELGIYMLN-SINPFRLEGQKTIAYEIYDQLQKVPDNVVLPVGNAGNISAIWKGFKEL 240
Query: 235 IEIGALEEMPKLIAVQTDRCNPIA---SEILGIKGECK-ETKALGLYVREPLMKPKVIRA 290
E G ++++P++I +Q + +P+A E K E ET A + + P K RA
Sbjct: 241 YEAGVIDKLPRMIGIQAEGASPLAKAWKEKRKFKPEKNPETLATAIRIGNPANWEKAWRA 300
Query: 291 IRESSGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLV 349
+ ES G +VS+EEI K +R+LA+ EG+F E +SA + LIKL+N+ +++ + VLV
Sbjct: 301 VEESGGMFEIVSDEEILKAQRILASKEGLFVEPASAASLAGLIKLLNEEKVDRDESFVLV 360
Query: 350 VTGSGLK 356
TG GLK
Sbjct: 361 TTGHGLK 367
>pir||E71136 threonine synthase (EC 4.2.99.2) PH0857 [similarity] - Pyrococcus
horikoshii >gi|3257268|dbj|BAA29951.1| (AP000003) 394aa
long hypothetical threonine synthase [Pyrococcus
horikoshii]
Length = 394
Score = 239 bits (603), Expect = 5e-62
Identities = 144/367 (39%), Positives = 220/367 (59%), Gaps = 13/367 (3%)
Query: 1 MKCPKCGREY-KAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIV 59
++C CGREY + E+ C CG LE+ D VDT + R +W+Y+ LP VKK V
Sbjct: 3 LRCVVCGREYPEDEVRYRCDCGGLLEVVIDLDKVDTSIFNGRDIKLWKYESWLP-VKKRV 61
Query: 60 SLQEGGTPLIK-ARISEQLNV-DVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVA 117
SL+EGGTPL + + + +L V +++ K+E NPTGSF+DR TV +S L + I A
Sbjct: 62 SLEEGGTPLYRLSNLENELKVKELYAKNEGANPTGSFKDRGMTVGVSKALELGMDKVICA 121
Query: 118 SDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYAKE 176
S GN +AS+AAY+A+A + +V+IP K+ GKL Q I GA++I + DDA+ E
Sbjct: 122 STGNTSASLAAYAAKAGIKAYVLIPSGKIALGKLAQAIIHGAKVIPIKGNFDDALRIVVE 181
Query: 177 LAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN--PTHVIVPTGSGSYLYSIYKGFKEL 234
++ G+Y + N LEGQKTIA+E+++++ P ++I+P G+ + +I+KGFKE+
Sbjct: 182 ASRELGIYMLN-SINPFRLEGQKTIAYEIYDQLGKVPDNIILPVGNAGNISAIWKGFKEM 240
Query: 235 IEIGALEEMPKLIAVQTDRCNPIASEILGIK----GECKETKALGLYVREPLMKPKVIRA 290
E G ++E+P++I +Q + +P+ K E ET A + + P K +A
Sbjct: 241 YEAGVIDELPRMIGIQAEGASPLVKAWKEKKKFQPEENPETVASAIRIGNPANWIKAWKA 300
Query: 291 IRESSGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLV 349
+ ES G VS+EEI + +RLLA+ EG+F E +SA + LIKL+ G I + + +L+
Sbjct: 301 VEESGGLFESVSDEEILRAQRLLASREGLFVEPASAASLAGLIKLLESGEISRNESYILI 360
Query: 350 VTGSGLK 356
TG GLK
Sbjct: 361 TTGHGLK 367
>gb|AAK41164.1| Threonine synthase (thrC-2) [Sulfolobus solfataricus]
Length = 396
Score = 233 bits (587), Expect = 4e-60
Identities = 154/371 (41%), Positives = 217/371 (57%), Gaps = 23/371 (6%)
Query: 1 MKCPKCGREYKAEIPPFCI----CGEELEITYDYSSVDTRKWKNRRPGVWRYKELLP-KV 55
MKC CG Y+ EI I CG LEI + D K R GVWRYKEL+ +
Sbjct: 1 MKCLNCG--YETEIDQSQILCPRCGGLLEIILEPPK-DFSFSKLRGRGVWRYKELIAGRY 57
Query: 56 KKIVSLQEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFI 115
K IVS+ EG TPLI+ S +N +++ K E NPTGSF+DR TVA+S + I
Sbjct: 58 KNIVSINEGNTPLIR---SSNINDNLYFKFEGLNPTGSFKDRGMTVAVSSAVSLNYKTVI 114
Query: 116 VASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYA 174
AS GN AAS AAY+ARA + F+V+P+ KV GKL Q I +G+ I+ S D A++
Sbjct: 115 AASTGNTAASAAAYAARAGIKSFIVLPKGKVALGKLAQSILYGSVILEVDGSFDVAMDAV 174
Query: 175 KELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN-PTHVIVPTGSGSYLYSIYKGFKE 233
L K + N LEGQKTIAFE+ EE+ P +VIVP G+ +Y+I+KGF E
Sbjct: 175 MRLYKDLKVVYPLNSFNPWRLEGQKTIAFEIAEELGVPDNVIVPVGNAGNIYAIWKGFNE 234
Query: 234 LIEIGALEEMPKLIAVQTDRCNPIASEILGIKG-------ECKETKALGLYVREPLMKPK 286
L++ G + +P++I +Q + +PI + I IKG E +T A + + +P+ K
Sbjct: 235 LVKTGVINTIPRMIGIQAEGASPIVTAI--IKGKDSPDFIENPDTVATAIRIGKPVNWQK 292
Query: 287 VIRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGFIEKGDR 345
++AIRES+GTA+ VS+ EI ++ LA EGI AE +SA + IK +N+ ++K ++
Sbjct: 293 AMKAIRESNGTAIAVSDSEILDAQKTLARKEGIGAEPASAAALAGYIKAINEKVVDKDEK 352
Query: 346 VVLVVTGSGLK 356
VL++TG LK
Sbjct: 353 TVLILTGHSLK 363
>pir||G69131 threonine synthase (EC 4.2.99.2) - Methanobacterium
thermoautotrophicum >gi|2621303|gb|AAB84759.1|
(AE000812) threonine synthase [Methanobacterium
thermoautotrophicum]
Length = 406
Score = 224 bits (565), Expect = 2e-57
Identities = 140/370 (37%), Positives = 212/370 (56%), Gaps = 16/370 (4%)
Query: 1 MKCPKCGREYKAEIPPFCI--CGEELEITYDYSSVDTRKWKNRRPGVWRYKELLP-KVKK 57
++C CGREY E + CG LE+ D V ++ RR +WRY+E +P +
Sbjct: 7 IRCISCGREYDLEEIIYTCRECGSVLEVECDVE-VSRDVFRGRRQNMWRYREFMPVDPSR 65
Query: 58 IVSLQEGGTPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIV 116
IVSL EGGTP ++ I +L +++++K E NPTGSF+DR +V I+ L +
Sbjct: 66 IVSLDEGGTPFVRCDTIGSELGIELYVKVEGSNPTGSFKDRGMSVGITKALELGVDTVGC 125
Query: 117 ASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYAK 175
AS GN +AS+AAY+ARA C V++P KV GKL Q + GAR++ + D+A+E
Sbjct: 126 ASTGNTSASLAAYAARAGLGCVVLLPSGKVALGKLAQAMFHGARVLSVRGNFDEALEAVT 185
Query: 176 ELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEI---NPTHVIVPTGSGSYLYSIYKGFK 232
ELA + LY + N LEGQK+I FE+ +++ +P +I+P G+ + +I+KG
Sbjct: 186 ELALMGELY-LLNSVNPFRLEGQKSIGFEIVDDLGWESPDRIILPVGNAGNISAIWKGIS 244
Query: 233 ELIEIGALEEMPKLIAVQTDRCNPIASEILGIKGECK-----ETKALGLYVREPLMKPKV 287
E G ++E P + +Q + +P+ + E + ET A + + P+ K
Sbjct: 245 EFYRAGFIDERPMMTGIQAEGASPVVEAFRKGRTEIEPVRDPETVATAIRIGAPVSYLKA 304
Query: 288 IRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGFIEKGDRV 346
+RAI ES G A V++EEI ++LLA EGI E +SA + LIKLV++G +++G+RV
Sbjct: 305 LRAIYESDGYAESVTDEEILSAQKLLARREGIGVEPASAASIAGLIKLVDEGVVDRGERV 364
Query: 347 VLVVTGSGLK 356
V VVTG LK
Sbjct: 365 VCVVTGHVLK 374
>pir||B72455 threonine synthase (EC 4.2.99.2) APE2286 [similarity] - Aeropyrum
pernix (strain K1) >gi|5105987|dbj|BAA81298.1|
(AP000064) 393aa long hypothetical threonine synthase
[Aeropyrum pernix]
Length = 393
Score = 210 bits (529), Expect = 3e-53
Identities = 136/363 (37%), Positives = 199/363 (54%), Gaps = 30/363 (8%)
Query: 3 CPKCGREYKAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIVSLQ 62
CPKCG + PF I G R GVWRY++LLP+ ++IV++
Sbjct: 16 CPKCG----GLLEPFYIDGGS----------------PRGRGVWRYRDLLPRPRRIVTMG 55
Query: 63 EGGTPLIKAR-ISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDGN 121
EG TPL+K +S + V+V+ K E NPTGSF+DR TV +S ++ + AS GN
Sbjct: 56 EGSTPLVKLENLSRAMGVEVYGKLEFLNPTGSFKDRGMTVGVSIASSFSYRLVVAASTGN 115
Query: 122 AAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKL 180
AAS+AAY+ RA ++IP+ + GKL Q+ A GA ++ S DDA++ A+ A+
Sbjct: 116 TAASMAAYARRAGLNPVILIPKGGIASGKLSQIAALGAWVLEVEGSFDDALDAARAAAEK 175
Query: 181 NGLYNVTPEENIVGLEGQKTIAFELWEEINPT-HVIVPTGSGSYLYSIYKGFKELIEIGA 239
Y++ N LEGQKTIAFE+ E+++ HV+VP G+ + +I+KGFKE +G
Sbjct: 176 GLAYSLN-SINPYRLEGQKTIAFEILEDVDRVDHVVVPVGNAGNISAIWKGFKEAGLLGL 234
Query: 240 LEEMPKLIAVQTDRCNPIASEILGIKGECK-----ETKALGLYVREPLMKPKVIRAIRES 294
P+++ VQ + +P+ + + T A + + P+ K + A+RES
Sbjct: 235 PSMRPRMVGVQAEGASPLYRAWVEKMDSVERVRNPSTIASAIRIGNPVNAAKALAAVRES 294
Query: 295 SGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGS 353
G + VS+ EI K LA NEG+ E +SA + LIKL N G I G VVL++TG
Sbjct: 295 DGLIISVSDAEILKAAATLAENEGMLVEPASAASLAGLIKLRNQGIIASGSSVVLILTGH 354
Query: 354 GLK 356
GLK
Sbjct: 355 GLK 357
>gi|11498914 threonine synthase (thrC-2) [Archaeoglobus fulgidus]
>gi|7436916|pir||C69414 threonine synthase (EC 4.2.99.2)
thrC-2 AF1316 [similarity] - Archaeoglobus fulgidus
>gi|2649262|gb|AAB89930.1| (AE001013) threonine synthase
(thrC-2) [Archaeoglobus fulgidus]
Length = 396
Score = 208 bits (525), Expect = 8e-53
Identities = 135/370 (36%), Positives = 208/370 (55%), Gaps = 13/370 (3%)
Query: 1 MKCPKCGREYKAEIPPFCICGEELEITYDYSSVDTR-KWKNRRPGVWRYKELLPKVKKIV 59
+KC +CG+E+ E C CG LE+ D V+ + VW+Y+ LLP V
Sbjct: 4 LKCIECGKEWGDENYT-CECGGLLEVEIDLDEVEIDFRLDGSNITVWKYRSLLPVKIDPV 62
Query: 60 SLQEGGTPLIKA-RISEQLNVD-VFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVA 117
+L+EGGTPL +A R+ +++ V V++K E NP+GSF+DR TV ++ L + A
Sbjct: 63 TLKEGGTPLYRAERLEKEVGVRRVYVKHEGLNPSGSFKDRGMTVGVTKALELGRKAVACA 122
Query: 118 SDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYAKE 176
S GN +AS+A Y+A+A + +V++P KV GK+ Q + GA++I + DDA+ +E
Sbjct: 123 STGNTSASMAMYAAKAGLKAYVLLPAGKVALGKVAQALMHGAKVIGIDGNFDDALRIVRE 182
Query: 177 LAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN-PTHVIVPTGSGSYLYSIYKGFKELI 235
+ +Y + N EGQKTIA+E+ +EI P V++P G+ + +IYKGFKEL
Sbjct: 183 VCSREPIY-LLNSINPFRPEGQKTIAYEIADEIGVPDRVVLPVGNAGNISAIYKGFKELR 241
Query: 236 EIGALEEMPKLIAVQTDRCNPIASEILGIKG-----ECKETKALGLYVREPLMKPKVIRA 290
E+G + MPK+ +Q + PI K E ET A + + P+ K +RA
Sbjct: 242 EVGLTDSMPKMTGIQAEGAAPIYRAFKEGKEDITPMENPETIATAIRIGNPVSAKKALRA 301
Query: 291 IRESSGTAVLVSEEEIEKGERLL-ANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLV 349
I ++G A +VS+ EI + ++ L A EGI E +SA + L KL +G ++ + +V V
Sbjct: 302 IYSTNGLAEVVSDSEIIEAQKFLAAKEGIGVEPASAASVAGLRKLAANGMLDPDETIVCV 361
Query: 350 VTGSGLKTGE 359
VTG+ LK E
Sbjct: 362 VTGNLLKDPE 371
>gi|11498161 threonine synthase (thrC-1) [Archaeoglobus fulgidus]
>gi|7436927|pir||G69318 threonine synthase (EC 4.2.99.2)
thrC-1 AF0551 [similarity] - Archaeoglobus fulgidus
>gi|2650068|gb|AAB90683.1| (AE001066) threonine synthase
(thrC-1) [Archaeoglobus fulgidus]
Length = 404
Score = 198 bits (499), Expect = 9e-50
Identities = 134/381 (35%), Positives = 199/381 (52%), Gaps = 20/381 (5%)
Query: 1 MKCPKCGREYKAEIPPFC-ICGEELEITYDYS----SVDTRKWKNRRPGVWRYKELLPKV 55
+KC CG EY E C +C LE YDY SV + R VWRY+E LP
Sbjct: 6 LKCRICGTEYPPEPLYHCSLCFGPLEAVYDYEGIKESVSKEEISKRVQSVWRYREFLPTN 65
Query: 56 KKIVSLQEGGTPLIKAR-ISEQLNV-DVFIKDETRNPTGSFRDRLATVAISYGLPYAKNG 113
V L G T I A+ + E L + ++++ D++ NPT SF+DR+ +VA++ L + +
Sbjct: 66 HDPVDLGAGFTRFIHAKNLGECLGLKNLYLLDDSTNPTYSFKDRVVSVAVTKALEFGMDV 125
Query: 114 FIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEY 173
AS GN A S+AA++A+A + F+ +P ++K K++Q + GA ++ + DDA
Sbjct: 126 VGCASTGNLAGSLAAHAAKAGLKAFIFVPAGIEKNKIVQALVHGATVVEVNGTYDDANRL 185
Query: 174 AKELAKLN---GLYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSI 227
A E+A+ N G N+ V EG KT+AFE E + P+ V+VP SG+ LYSI
Sbjct: 186 AAEIAEKNRNWGFVNINLRPYYV--EGSKTLAFEAAERLGWEAPSQVVVPVASGALLYSI 243
Query: 228 YKGFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKG----ECKETKALGLYVREPLM 283
Y+GFKEL +G ++ P+A + + E T L + P
Sbjct: 244 YRGFKELETVGLIDSAEVAFNAAQPEGFPVARAVKERRQVEPIERFSTIVHSLAIGNPAD 303
Query: 284 KPKVIRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGFIEK 342
+ S G A S+ E+ + +LLA EGIF EL+ V + ALI+LV DG I++
Sbjct: 304 GIFAKEVVERSGGYAEDPSDAEVIEAVKLLARTEGIFTELAGGVTVAALIRLVEDGRIDR 363
Query: 343 GDRVVLVVTGSGLKTGEGGRE 363
++VV +TG+GLKT E E
Sbjct: 364 DEKVVAYLTGNGLKTAEAVAE 384
>pir||D70354 threonine synthase (EC 4.2.99.2) thrC1 [similarity] - Aquifex
aeolicus >gi|2983225|gb|AAC06822.1| (AE000698) threonine
synthase [Aquifex aeolicus]
Length = 409
Score = 193 bits (485), Expect = 4e-48
Identities = 139/397 (35%), Positives = 203/397 (51%), Gaps = 20/397 (5%)
Query: 1 MKCPKCGREYKAEIPPFC-ICGEELEITYDYS----SVDTRKWKNRRPGVWRYKELLPKV 55
+KC +CGREY AE C C LE+ YDY ++ K + +WRY +LLP
Sbjct: 7 LKCRECGREYPAEPIHVCEFCFGPLEVVYDYDEIKKNISREKIEKGPKSLWRYVDLLPVE 66
Query: 56 KKIVSLQEGGTPLIKA-RISEQLNV-DVFIKDETRN-PTGSFRDRLATVAISYGLPYAKN 112
V L G TPL KA ++ E L + +++IKD++ N PT SF+DR+ +VA+S + +
Sbjct: 67 NPTVGLTAGFTPLKKAEKLGEVLGLKNLYIKDDSVNHPTLSFKDRVVSVALSKAKEFGFD 126
Query: 113 GFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIE 172
AS GN A SVAA+SA+A CFV IP ++ K+I + F ++ + DD
Sbjct: 127 TAACASTGNLANSVAAHSAQAGMNCFVFIPANLETQKIIGSLVFNPTVVAVEGNYDDVNR 186
Query: 173 YAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSIYK 229
E+A G V EG KT+AFE+ E++ P V+ P SGS I+K
Sbjct: 187 LCSEIANDLGWAFVNINIRPYYAEGSKTLAFEVAEQLGWRAPDVVVAPAASGSLYTKIWK 246
Query: 230 GFKELIEIGALEEM-PKLIAVQTDRCNPIASEILG----IKGECKETKALGLYVREPLMK 284
GF EL ++G ++E+ ++ Q + C+PIA IK T A + + P
Sbjct: 247 GFNELKKVGLIDEVKTRMYGAQAEGCSPIAQAWREGRDFIKPVKPNTIAKSIAIGNPADG 306
Query: 285 PKVIRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGFIEKG 343
++ RES+G ++EEI +G +LLA EGIF E + + L K V G I
Sbjct: 307 IYALQVARESNGAWETATDEEIIEGIKLLAQTEGIFTETAGGTTIAVLKKYVEKGMISPE 366
Query: 344 DRVVLVVTGSGLKTGEGGREKFGIGGTKLEILKILKD 380
+ VV+ +TG+G KT E K T ++I LKD
Sbjct: 367 ETVVVYITGNGYKTMEVLEGKL---STPVKIKPTLKD 400
>gb|AAG20514.1| (AE005123) threonine synthase; ThrC1 [Halobacterium sp. NRC-1]
Length = 415
Score = 184 bits (462), Expect = 2e-45
Identities = 133/376 (35%), Positives = 192/376 (50%), Gaps = 26/376 (6%)
Query: 1 MKCPKCGREYK--AEIPPFCI-CGEELEITY-DYSSVDTRKWKNRRPGVWRYKELLPKVK 56
+ C +CG A++ C C LE Y DY + + GVWRY + LP V
Sbjct: 20 LACVECGGTLAPFADVVYRCPECSGLLEARYADYPGFEAFSGR----GVWRYADALP-VD 74
Query: 57 KIVSLQEGGTPLIKAR-ISEQLNV-DVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGF 114
VS+QEG TPL +A ++ V DV +K E NPTGSF+DR TV + +
Sbjct: 75 AGVSVQEGDTPLYEAPDVAAAAGVADVRVKHEGMNPTGSFKDRGMTVGVRVAERLGVDRL 134
Query: 115 IVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEY 173
AS GN +A++AAY RA+ V++P KV GK+ Q GARI + DD ++
Sbjct: 135 ACASTGNTSAALAAYGGRADTPVLVLLPAGKVAAGKIAQAALHGARICEVDGNFDDCLDV 194
Query: 174 AKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN------PTHVIVPTGSGSYLYSI 227
ELA Y + N LEGQKTIAFE+ E+ P +++P G+ ++
Sbjct: 195 VAELAARGEAYLLN-SLNPFRLEGQKTIAFEILEQSRTATGEWPDRIVLPVGNAGNTAAL 253
Query: 228 YKGFKELIEIGAL--EEMPKLIAVQTDRCNPIASEILGIKGECK-----ETKALGLYVRE 280
YK F+EL+ GA+ +EMP L VQ P+ + +G + ET+A + +
Sbjct: 254 YKAFRELVAAGAMTDDEMPALTGVQAAGAAPMVEAVEAGRGRTERWTDVETRATAIRIGN 313
Query: 281 PLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFI 340
P+ PK + + E+ GTAV VS++ I +R+LA +GI E +SA + L+ L G I
Sbjct: 314 PVNAPKALPGVYETGGTAVAVSDDRITDAQRMLAGDGIGVEPASAASVAGLLALRERGEI 373
Query: 341 EKGDRVVLVVTGSGLK 356
+RVV + TG LK
Sbjct: 374 SADERVVCLTTGHLLK 389
>sp|Q10610|THRC_MYCTU PROBABLE THREONINE SYNTHASE >gi|7436919|pir||C70773 threonine
synthase (EC 4.2.99.2) thrC [similarity] - Mycobacterium
tuberculosis (strain H37RV) >gi|1322419|emb|CAA97760.1|
(Z73419) thrC [Mycobacterium tuberculosis]
Length = 360
Score = 183 bits (459), Expect = 4e-45
Identities = 109/303 (35%), Positives = 175/303 (56%), Gaps = 8/303 (2%)
Query: 59 VSLQEGGTPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVA 117
V+L EGGTPLI A +S+Q + +K E NPTGSF+DR T+A++ L + + + A
Sbjct: 32 VTLLEGGTPLIAATNLSKQTGCTIHLKVEGLNPTGSFKDRGMTMAVTDALAHGQRAVLCA 91
Query: 118 SDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYAKE 176
S GN +AS AAY+ARA C V+IP+ K+ GKL Q + GA+II+ + DD +E A++
Sbjct: 92 STGNTSASAAAYAARAGITCAVLIPQGKIAMGKLAQAVMHGAKIIQIDGNFDDCLELARK 151
Query: 177 LAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN--PTHVIVPTGSGSYLYSIYKGFKEL 234
+A ++ N V +EGQKT AFE+ + + P +P G+ + + +KG+ E
Sbjct: 152 MAADFPTISLVNSVNPVRIEGQKTAAFEIVDVLGTAPDVHALPVGNAGNITAYWKGYTEY 211
Query: 235 IEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRES 294
++G ++++P+++ Q P+ +LG ET A + + P + A ++S
Sbjct: 212 HQLGLIDKLPRMLGTQAAGAAPL---VLGEPVSHPETIATAIRIGSPASWTSAVEAQQQS 268
Query: 295 SGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGS 353
G + S+EEI L+A EG+F E +SA + L+K ++DG++ +G VV VTG+
Sbjct: 269 KGRFLAASDEEILAAYHLVARVEGVFVEPASAASIAGLLKAIDDGWVARGSTVVCTVTGN 328
Query: 354 GLK 356
GLK
Sbjct: 329 GLK 331
>pir||G72364 threonine synthase (EC 4.2.99.2) TM0546 [similarity] - Thermotoga
maritima (strain MSB8)
>gi|4981060|gb|AAD35631.1|AE001729_11 (AE001729)
threonine synthase [Thermotoga maritima]
Length = 348
Score = 181 bits (454), Expect = 2e-44
Identities = 117/319 (36%), Positives = 177/319 (54%), Gaps = 15/319 (4%)
Query: 47 RYKELLPKVKK--IVSLQEGGTPLIK-ARISEQLNVDVFIKDETRNPTGSFRDRLATVAI 103
+Y+ LP K ++SL EG TPLI +S +L +++++K E NPTGSF+DR VA+
Sbjct: 8 KYRRFLPVTDKTPMLSLNEGNTPLIPLVNMSRELGINIYVKYEGSNPTGSFKDRGMVVAV 67
Query: 104 SYGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIR 162
+ L + AS GN +AS AAY+ARA + V+IP K+ GKL Q + +GA +++
Sbjct: 68 AKALEEGSKAIMCASTGNTSASAAAYAARAGIKAIVLIPEGKIALGKLAQAMIYGAVVLQ 127
Query: 163 YGESVDDAIEYAKELAKLNGLYNVTPEENI--VGLEGQKTIAFELWEEIN--PTHVIVPT 218
+ D +E KE + Y +T +I LEGQKT AFE+ +E+ P + +P
Sbjct: 128 VRGNFDKCLELVKE---ITSKYPITLVNSINPYRLEGQKTAAFEIVDELGDAPDYHFIPV 184
Query: 219 GSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYV 278
G+ + + + G+KE + G ++PK++ Q + PI + G E ET A + +
Sbjct: 185 GNAGNISAYWMGYKEYYQHGFSTKLPKMMGFQAEGAAPI---VRGHPIENPETVATAIRI 241
Query: 279 REPLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVND 337
P K +RA ES G +VS+EEI + +RLLA EGIF E +SA + L+K
Sbjct: 242 GNPANWEKAVRARDESGGDIDMVSDEEILRAQRLLAQKEGIFCEPASAASIAGLLKKHRQ 301
Query: 338 GFIEKGDRVVLVVTGSGLK 356
G G+ VV +TG+GLK
Sbjct: 302 GIFRGGEIVVCTLTGNGLK 320
>sp|P45837|THRC_MYCLE PROBABLE THREONINE SYNTHASE >gi|7436934|pir||T09991 threonine
synthase (EC 4.2.99.2) - Mycobacterium leprae
>gi|699329|gb|AAA63090.1| (U15186) threonine synthase
[Mycobacterium leprae]
Length = 360
Score = 177 bits (444), Expect = 2e-43
Identities = 108/303 (35%), Positives = 172/303 (56%), Gaps = 8/303 (2%)
Query: 59 VSLQEGGTPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVA 117
V+L EGGTPLI A R+SEQ + +K E NPTGSF+DR T+A++ L + + A
Sbjct: 32 VTLLEGGTPLIAAPRLSEQTGCTIHLKVEGLNPTGSFKDRGMTMAVTDALARGQRAVLCA 91
Query: 118 SDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIEYAKE 176
S GN +AS AAY+ARA C V+IP+ K+ GKL Q + GA+II+ + DD +E A++
Sbjct: 92 STGNTSASAAAYAARAGITCAVLIPQGKIAMGKLAQAVMHGAKIIQIDGNFDDCLELARK 151
Query: 177 LAKLNGLYNVTPEENIVGLEGQKTIAFELWEEINPT-HV-IVPTGSGSYLYSIYKGFKEL 234
+A + ++ N V +EGQKT FE+ + + HV +P G+ + + +KG+ E
Sbjct: 152 MAADFPMISLVNSVNPVRIEGQKTAVFEIVDALGTAPHVHALPVGNAGNITAYWKGYTEY 211
Query: 235 IEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRES 294
G ++ +P+++ Q P+ +LG ET A + + P + A ++S
Sbjct: 212 HADGLIDRLPRMLGTQAAGAAPL---VLGEPVSHPETIATAIRIGSPASWTSAVEAQQQS 268
Query: 295 SGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGS 353
G + ++EEI L+A EG+F E +SA + L+K ++ G++ +G VV +TG+
Sbjct: 269 KGRFLAATDEEILAAYHLVARAEGVFVEPASAASIAGLLKAIDGGWVARGSTVVCTITGN 328
Query: 354 GLK 356
GLK
Sbjct: 329 GLK 331
>sp|P04990|THRC_BACSU THREONINE SYNTHASE >gi|80429|pir||A25364 threonine synthase (EC
4.2.99.2) thrC - Bacillus subtilis
>gi|40211|emb|CAA28270.1| (X04603) threonine synthase
[Bacillus subtilis] >gi|2635722|emb|CAB15215.1| (Z99120)
threonine synthase [Bacillus subtilis]
Length = 352
Score = 174 bits (436), Expect = 2e-42
Identities = 113/320 (35%), Positives = 180/320 (55%), Gaps = 14/320 (4%)
Query: 45 VWRYKELLPKVKKI--VSLQEGGTPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATV 101
+ +YKE LP + ++L EG TPLI ++SEQL +++ +K E NPTGSF+DR +
Sbjct: 6 IHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIELHVKTEGVNPTGSFKDRGMVM 65
Query: 102 AISYGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARI 160
A++ + + AS GN +A+ AAY+ARA +C V+IP K+ GKL Q + +GA I
Sbjct: 66 AVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVIIPNGKIAFGKLAQAVMYGAEI 125
Query: 161 IRYGESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN--PTHVIVPT 218
I + DDA++ + + + + + V N +EGQKT AFE+ E++ P + +P
Sbjct: 126 IAIDGNFDDALKIVRSICEKSPIALVN-SVNPYRIEGQKTAAFEVCEQLGEAPDVLAIPV 184
Query: 219 GSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIA-SEILGIKGECKETKALGLY 277
G+ + + +KGFKE E +PK+ + + I +E++ E ET A +
Sbjct: 185 GNAGNITAYWKGFKEYHEKNG-TGLPKMRGFEAEGAAAIVRNEVI----ENPETIATAIR 239
Query: 278 VREPLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVMPALIKLVN 336
+ P K ++A ES+G V+++EI +L+A EG+FAE S + ++K V
Sbjct: 240 IGNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVK 299
Query: 337 DGFIEKGDRVVLVVTGSGLK 356
G I KG +VV V+TG+GLK
Sbjct: 300 SGEIPKGSKVVAVLTGNGLK 319
>pir||H70338 threonine synthase (EC 4.2.99.2) - Aquifex aeolicus
>gi|2983084|gb|AAC06690.1| (AE000689) threonine synthase
[Aquifex aeolicus]
Length = 352
Score = 173 bits (433), Expect = 5e-42
Identities = 114/327 (34%), Positives = 184/327 (55%), Gaps = 14/327 (4%)
Query: 40 NRRPGVWR-YKELLPKVKK--IVSLQEGGTPLIKA-RISEQLNVD--VFIKDETRNPTGS 93
NR G+ + YK+ LP + IV+L EG TPLI+A ++ + +++K E NPTGS
Sbjct: 2 NRWQGIIKQYKKYLPVDENTPIVTLYEGNTPLIEADNLARAIGFKGKIYLKYEGLNPTGS 61
Query: 94 FRDRLATVAISYGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQ 152
F+DR T+AIS + K I AS GN +AS AAY+ARA +V++P+ V GKL Q
Sbjct: 62 FKDRGMTLAISKAVEAGKRAVICASTGNTSASAAAYAARAGLRAYVLLPKGAVAIGKLSQ 121
Query: 153 MIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN-- 210
+ +GA+++ + DDA+ +++ + N + N +EGQKT AFE+ + +
Sbjct: 122 AMIYGAKVLAIQGTFDDALNIVRKIGE-NFPVEIVNSVNPYRIEGQKTAAFEICDTLGEA 180
Query: 211 PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKE 270
P + +P G+ + + +KGFK E G + ++P+++ Q + PI + G + +
Sbjct: 181 PDYHFIPVGNAGNITAYWKGFKIYYEEGKITKLPRMMGWQAEGAAPI---VKGYPIKNPQ 237
Query: 271 TKALGLYVREPLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVMP 329
T A + + P ++A +ES G VS+ EI +L+A+ EG+F E +SA +
Sbjct: 238 TIATAIKIGNPYSWKSALKAAQESGGKIDAVSDSEILYAYKLIASTEGVFCEPASAASVA 297
Query: 330 ALIKLVNDGFIEKGDRVVLVVTGSGLK 356
LIKLV +GF + G+ V +TG+GLK
Sbjct: 298 GLIKLVREGFFKGGEVVTCTLTGNGLK 324
>emb|CAB93053.1| (AL357432) putative threonine synthase [Streptomyces coelicolor
A3(2)]
Length = 428
Score = 171 bits (429), Expect = 1e-41
Identities = 118/384 (30%), Positives = 192/384 (49%), Gaps = 31/384 (8%)
Query: 1 MKCPKCGREYKAEIPPFCICGE---ELEITYDYSSVDTRKWKNR----RPGVWRYKELLP 53
+ C +CG ++ + P C E LEI YD+S D + + R +WRY LLP
Sbjct: 23 LSCRECG--HRVPLGPVFACEECFGPLEIAYDFSDYDAEELRKRIEAGPANIWRYAPLLP 80
Query: 54 KVKKIVS---LQEGGTPLIKA-RISEQLNVD--VFIKDETRNPTGSFRDRLATVAISYGL 107
+ + + G T L++A ++ +L V +++KD++ NPT SF+DR+ AI
Sbjct: 81 VPADVATKPNINPGWTKLVQADNLARELGVTGGLYVKDDSGNPTHSFKDRVVAQAIEAAR 140
Query: 108 PYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESV 167
+ +S GN A +V A +ARA V IP +++GK++ +G ++ +
Sbjct: 141 AFGFTTLSCSSTGNLAGAVGAAAARAGFRSCVFIPHDLEQGKVVMAAVYGGELVGIEGNY 200
Query: 168 DDAIEYAKEL-----AKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTG 219
DD + EL + G NV EG KT+A+E+ E++ P ++VP
Sbjct: 201 DDVNRFCSELIGDPAGEGWGFVNVNLRPYYA--EGSKTLAYEICEQLGWRLPDQIVVPIA 258
Query: 220 SGSYLYSIYKGFKELIEIGALEEMP-KLIAVQTDRCNPIASEILG----IKGECKETKAL 274
SGS L I KG KELIE+G +E+ P K+ Q + C+P+++ ++ + T A
Sbjct: 259 SGSQLTKIDKGLKELIELGLVEDRPYKIFGAQAEGCSPVSTAYKAGHDVVRPQKPNTIAK 318
Query: 275 GLYVREPLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIK 333
L + P P V+ R + G V++E++ +LLA EGIFAE + V + K
Sbjct: 319 SLAIGNPADGPYVLDIARRTGGAVEDVTDEQVVDAIKLLARTEGIFAETAGGVTVGVTRK 378
Query: 334 LVNDGFIEKGDRVVLVVTGSGLKT 357
L+ +G ++ V++ TG GLKT
Sbjct: 379 LIENGVLDPSLTTVVLNTGDGLKT 402
>sp|P09123|THRC_BRELA THREONINE SYNTHASE >gi|68321|pir||DWFKTG threonine synthase (EC
4.2.99.2) - Corynebacterium glutamicum
>gi|541381|pir||S41448 threonine synthase (EC 4.2.99.2)
- Bacillus sp >gi|452398|emb|CAA82669.1| (Z29562)
threonine synthase [Bacillus sp.]
Length = 352
Score = 168 bits (422), Expect = 9e-41
Identities = 122/344 (35%), Positives = 179/344 (51%), Gaps = 17/344 (4%)
Query: 47 RYKELLPKVKKI--VSLQEGGTPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAI 103
+Y LP +K VSL EG TPLI IS+QL V ++ K E NPTGSF+DR +A+
Sbjct: 8 QYASYLPVNEKTPDVSLMEGNTPLIPLLNISKQLGVQLYGKYEGANPTGSFKDRGMVMAV 67
Query: 104 SYGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIR 162
+ I AS GN +AS AAY+AR +C +VIP K+ GKL Q +A+GA II
Sbjct: 68 AKAKEEGSEAIICASTGNTSASAAAYAARLGMKCIIVIPEGKIAHGKLAQAVAYGAEIIS 127
Query: 163 YGESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN--PTHVIVPTGS 220
+ DDA++ + +A + + N +EGQKT AFE+ +++ P + +P G+
Sbjct: 128 IEGNFDDALKAVRNIAAEEPI-TLVNSVNPYRIEGQKTAAFEICDQLQNAPDVLAIPVGN 186
Query: 221 GSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVRE 280
+ + +KGF E + E+ K + A+ + G E ET A + +
Sbjct: 187 AGNITAYWKGFCEYEK----EKGYKKPRIHGFEAEGAAAIVKGHVIEEPETIATAIRIGN 242
Query: 281 PLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGF 339
P + A +S G +VS+EEI RLLA EG+FAE S + +IK V G
Sbjct: 243 PASWSYAVEAAEQSHGEIDMVSDEEILHAYRLLAKTEGVFAEPGSNASLAGVIKHVESGK 302
Query: 340 IEKGDRVVLVVTGSGLKTGEGGREKFGIGGTKLEILKILKDGEK 383
I+KG+ VV V+TG+GLK + I +L+I + D E+
Sbjct: 303 IKKGETVVAVLTGNGLKDPD-----IAISSNQLDIASVSNDIEQ 341
>gb|AAK40813.1| Threonine synthase (thrC-1) [Sulfolobus solfataricus]
Length = 345
Score = 162 bits (405), Expect = 9e-39
Identities = 112/359 (31%), Positives = 184/359 (51%), Gaps = 30/359 (8%)
Query: 3 CPKCGREYKAEIPPFCI-CGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIVSL 61
C KC +E ++ C CG EI D+ KN G +++ P +K +SL
Sbjct: 10 CMKCRKERESIYEIKCNKCGGPFEILIDFEFD-----KNSERGFPYSEKIFPYIKHFISL 64
Query: 62 QEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDGN 121
EG TPLIK +++ K + NP+GS++DR A +SY S GN
Sbjct: 65 GEGRTPLIKKG-------NIWFKLDFLNPSGSYKDRGAVTLVSYLAEKGVKQISEDSSGN 117
Query: 122 AAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLN 181
A +++AAYSA A E ++ +P GKL Q+ ++GA ++R S +D + A+
Sbjct: 118 AGSAIAAYSAAAGIEAYIFVPETAKGGKLKQIESYGAHVVRVRGSREDVAKAAENSGYYY 177
Query: 182 GLYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSIYKGFKELIEIG 238
+ + P+ +G +T+A+E+ ++++ P +V +P +G+ L +YKGFK L++ G
Sbjct: 178 ASHVLQPQFR----DGIRTLAYEIVKDLDWKIPNYVFIPVSAGTLLLGVYKGFKHLLDSG 233
Query: 239 ALEEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGTA 298
+ EMPK++AVQT++ P+ ++ I K + +P ++ + ++S
Sbjct: 234 VISEMPKIVAVQTEQVMPLCAKFKKI-SYTPPDKVTSIADALVSTRPFLLDYMVKASSEC 292
Query: 299 VLVSEEEIEKGERLLANEGIFAELSSAVVMPALIKL-VNDGFIEKGDRVVLVVTGSGLK 356
++VS+ EI + + LA G+ E SSA V A K VND VLV+TGSGLK
Sbjct: 293 IVVSDNEIVEAWKELAKMGLLVEYSSATVFAAYKKYSVND--------AVLVLTGSGLK 343
>pir||S77200 threonine synthase (EC 4.2.99.2) 1 - Synechocystis sp. (strain PCC
6803) >gi|1652839|dbj|BAA17758.1| (D90908) threonine
synthase [Synechocystis sp.]
Length = 433
Score = 157 bits (392), Expect = 3e-37
Identities = 114/368 (30%), Positives = 186/368 (49%), Gaps = 20/368 (5%)
Query: 19 ICGEE---LEITYDYSSVD---TRKWKNRRPG-VWRYKELLPKV-KKIVSLQEGGTPLIK 70
IC E LE+ YDY + TR+ P +WRY+ LP K ++ + G TPL+K
Sbjct: 34 ICEETFAPLEVAYDYDLIRRHVTRETIEAGPNSIWRYRAFLPVTGKDVIDVGTGMTPLVK 93
Query: 71 A-RISEQLNV-DVFIKDETRN-PTGSFRDRLATVAISYGLPYAKNGFIVASDGNAAASVA 127
+ R++ +L + +++IK++ N PT SF+DR+ +VA++ AS GN A S A
Sbjct: 94 SHRLARRLGLKNLYIKNDAVNMPTLSFKDRVVSVALTRARELGFTTVSCASTGNLANSTA 153
Query: 128 AYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVT 187
A +A A +C V IP ++ GK++ + + ++ + D E+ G V
Sbjct: 154 AIAAHAGLDCCVFIPADLEAGKVLGTLIYNPTLMAVKGNYDQVNRLCCEVGNSYGWGFVN 213
Query: 188 PEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMP 244
EG KT+ FE+ E++ P H++ P SGS I+KGF+E I++G +++ P
Sbjct: 214 INLRPYYSEGSKTLGFEVAEQLGWKLPDHIVAPLASGSLFTKIHKGFQEFIKVGLVDDKP 273
Query: 245 -KLIAVQTDRCNPIASEILG----IKGECKETKALGLYVREPLMKPKVIRAIRESSGTAV 299
+ Q + C+PIA+ +K T A + + P + R+++G
Sbjct: 274 VRFSGAQAEGCSPIATAFQEGRDFVKPVKPNTIAKSIAIGNPADGVYALDIARKTNGNIE 333
Query: 300 LVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLKTG 358
V++ EI +G +LLA EGIF E + + L KLV G I+ + V+ +TG+GLKT
Sbjct: 334 SVNDAEIIEGIKLLAETEGIFTETAGGTTVAVLKKLVEAGKIDPEETTVVYITGNGLKTQ 393
Query: 359 EGGREKFG 366
E + G
Sbjct: 394 EAVQSHVG 401
>dbj|BAB07140.1| (AP001518) threonine synthase [Bacillus halodurans]
Length = 354
Score = 155 bits (387), Expect = 1e-36
Identities = 107/317 (33%), Positives = 170/317 (52%), Gaps = 14/317 (4%)
Query: 48 YKELLPKVKK--IVSLQEGGTPLIKAR-ISEQLNVDVFIKDETRNPTGSFRDRLATVAIS 104
YKE LP ++ +++L EG TPLI +S++ V ++K E NPTGSF+DR +A++
Sbjct: 11 YKEFLPVTEETPLLTLGEGNTPLIPLENLSKEWGVKAYVKYEGANPTGSFKDRGMVMAVA 70
Query: 105 YGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRY 163
I AS GN +A+ AAY ARA C VVIP K+ GKL Q + +GA ++
Sbjct: 71 KAKEEGSRTIICASTGNTSAAAAAYGARAGLRCIVVIPEGKIALGKLAQAVMYGAEVLEI 130
Query: 164 GESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN--PTHVIVPTGSG 221
+ D A++ + +++ + + N +EGQKT AFE+ + + P + +P G+
Sbjct: 131 KGNFDHALDIVRSISEKEPI-TLVNSVNPYRIEGQKTSAFEICDALGQAPDVLAIPVGNA 189
Query: 222 SYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIA-SEILGIKGECKETKALGLYVRE 280
+ + +KGFKE E +P++ + + I ++++ E ET A + +
Sbjct: 190 GNITAYWKGFKEYHEKKG-TGLPQMRGFEAEGAAAIVRNQVI----EEPETIATAIRIGN 244
Query: 281 PLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLA-NEGIFAELSSAVVMPALIKLVNDGF 339
P + A ES+G V++EEI +LLA EG+FAE +S + L K + G
Sbjct: 245 PASWTYAVEAAAESNGKIDEVTDEEILAAYQLLAQKEGVFAEPASCASIAGLRKQIASGE 304
Query: 340 IEKGDRVVLVVTGSGLK 356
I+KG VV V+TG+GLK
Sbjct: 305 IKKGSTVVCVLTGNGLK 321
>pir||E75172 probable threonine synthase (EC 4.2.99.2) thrc-2 PAB0369
[similarity] - Pyrococcus abyssi (strain Orsay)
>gi|5457970|emb|CAB49460.1| (AJ248284) related threonine
synthase (thrC-2) [Pyrococcus abyssi]
Length = 343
Score = 152 bits (381), Expect = 6e-36
Identities = 118/354 (33%), Positives = 176/354 (49%), Gaps = 21/354 (5%)
Query: 3 CPKCGREYKAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIV-SL 61
C KCGR + + C CG L + Y+S R + + R RY + LP + L
Sbjct: 4 CTKCGRTFDEKFLLRCTCGGTLLVKRHYTSFSPRNYLDMR----RYIDYLPVDSVFLPKL 59
Query: 62 QEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDGN 121
TP++K + D+++K + PTGSF+DR V I+ N ++ S GN
Sbjct: 60 TPAITPIVKD------SEDLYLKLDYLQPTGSFKDRGTYVTIAKLKEEGINEVVIDSSGN 113
Query: 122 AAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLN 181
A S+AAY + V + +K K+ ++ A A++ E A ++
Sbjct: 114 AGISLAAYGLSEGIKVHVFLSYDANKEKISRLSALNAKLHFVDGDRMKVHEEAIRFSRDG 173
Query: 182 GLYNVTPEENIVGLEGQKTIAFELWEEIN-PTHVIVPTGSGSYLYSIYKGFKELIEIGAL 240
L V+ N LEG KTIAFE++E+I P +V +P GSG+ I+KGFKELIE+G +
Sbjct: 174 NLTYVSHWMNPYFLEGTKTIAFEIYEQIKLPDYVFIPVGSGTAFLGIWKGFKELIEMGEI 233
Query: 241 EEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVL 300
E MP L+AVQ + E L + + K T A G+ + EP ++ +AI+ESSG +
Sbjct: 234 ESMPSLVAVQAE-----GFESLCKRSKRKNTLADGIAIPEPPRIEEMKQAIKESSGFCIS 288
Query: 301 VSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRV-VLVVTGS 353
V +EE L GI E +SAV + A K +E D V +L++TGS
Sbjct: 289 VGKEETLASLHWLRKRGILVEGTSAVTLAAYWKAKE---LELIDGVSLLILTGS 339
>pir||C71042 probable threonine synthase (EC 4.2.99.2) PH1627 [similarity] -
Pyrococcus horikoshii >gi|3258056|dbj|BAA30739.1|
(AP000006) 346aa long hypothetical threonine synthase
[Pyrococcus horikoshii]
Length = 346
Score = 149 bits (373), Expect = 5e-35
Identities = 110/358 (30%), Positives = 177/358 (48%), Gaps = 29/358 (8%)
Query: 3 CPKCGREYKAEIPPFCICGEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIV-SL 61
C KCGR+++ + C CG L + Y S RK+ + R RY + LP +K + SL
Sbjct: 7 CSKCGRKFEEKFILRCTCGGTLLVKRKYDSFAPRKFLDIR----RYIDYLPVDEKFLPSL 62
Query: 62 QEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDGN 121
G TP+ K ++ +V++K + PTGSF+DR V ++ + ++ S GN
Sbjct: 63 TPGITPISK------ISDEVYLKLDYLQPTGSFKDRGTYVTVAKIKEEGIDEVVIDSSGN 116
Query: 122 AAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLN 181
A S+A Y + V + K+ ++ + AR+ E A + ++
Sbjct: 117 AGLSLATYGMAEGIKVHVFLSYNAKPEKIARLNSVNARLHFVEGDRMRVHEEAVKFSERT 176
Query: 182 GLYNVTPEENIVGLEGQKTIAFELWEEIN-PTHVIVPTGSGSYLYSIYKGFKELIEIGAL 240
G+ + N LEG KTIAFE++E+I P ++ VP GSG+ I+KGFKEL+E+G +
Sbjct: 177 GIPYASHWLNPYFLEGTKTIAFEIYEQIGIPRYIFVPVGSGTAFLGIWKGFKELLEMGEI 236
Query: 241 EEMPKLIAVQTDRCNPIASEILGIKGECKETKAL-----GLYVREPLMKPKVIRAIRESS 295
+MP L+AVQ + G + CK +K++ G+ + P ++ A+RE+S
Sbjct: 237 NKMPSLVAVQAE----------GYESLCKRSKSVNKLADGIAIPNPPRIEEMKVALRETS 286
Query: 296 GTAVLVSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGS 353
G + V EEE G L G F E +SA + K G ++ +L++TGS
Sbjct: 287 GFCISVGEEETLAGYHWLRRNGFFIEETSATALAGYWKAREKGLVKGSS--LLLLTGS 342
>sp|P74193|THRC_SYNY3 THREONINE SYNTHASE >gi|7436917|pir||S75823 threonine synthase (EC
4.2.99.2) 2 - Synechocystis sp. (strain PCC 6803)
>gi|1653368|dbj|BAA18282.1| (D90913) threonine synthase
[Synechocystis sp.]
Length = 382
Score = 146 bits (365), Expect = 4e-34
Identities = 114/320 (35%), Positives = 170/320 (52%), Gaps = 19/320 (5%)
Query: 48 YKELLPKVKK--IVSLQEGGTPLIKA-RISEQLNVDV--FIKDETRNPTGSFRDRLATVA 102
Y+ LP + +V+L EG TPLI A I++++ DV F+K + NPTGSF+DR T+A
Sbjct: 41 YRPYLPVSDQTPVVTLLEGNTPLIPAPAIAKRIGKDVRVFVKYDGLNPTGSFKDRGMTMA 100
Query: 103 ISYGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIP-RKVDKGKLIQMIAFGARII 161
IS I AS GN +A+ AAY+ RA FV+IP V GKL Q + +GA +I
Sbjct: 101 ISKAKEAGAKAVICASTGNTSAAAAAYARRAGLRAFVIIPDGYVALGKLGQALIYGAEVI 160
Query: 162 RYGESVDDAIEYAKELAKLNGLYNVT--PEENIVGLEGQKTIAFELWEEIN--PTHVIVP 217
+ DDA+ ++L++ Y VT N LEGQKT AFE+ + + P + +P
Sbjct: 161 AIDGNFDDALTTVRQLSE---HYPVTLVNSVNPYRLEGQKTAAFEIVDVLGQAPDWLCIP 217
Query: 218 TGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKETKALGLY 277
G+ + + + GF + E+G + +P+++ Q P I G ET A +
Sbjct: 218 VGNAGNITAYWMGFCQYKELGKGDRLPRMMGFQAAGSAPF---IQGQPISHPETLATAIR 274
Query: 278 VREPLMKPKVIRAIRESSGTAVLVSEEEIEKGERLL-ANEGIFAELSSAVVMPALIKLVN 336
+ P K A RES+G V++ EI + R+L A EG+F E +SA + L+K +
Sbjct: 275 IGNPANWDKAWAASRESNGEFHPVTDAEILEAYRILAAEEGVFCEPASAASVAGLLKQKD 334
Query: 337 DGFIEKGDRVVLVVTGSGLK 356
+ G VV V+TG+GLK
Sbjct: 335 Q--VPAGATVVCVLTGNGLK 352
>gb|AAG19064.1| (AE005005) threonine synthase; ThrC2 [Halobacterium sp. NRC-1]
Length = 398
Score = 145 bits (363), Expect = 8e-34
Identities = 113/378 (29%), Positives = 181/378 (46%), Gaps = 19/378 (5%)
Query: 1 MKCPKCGREYKAEIPPFCI--CGEELEITYDYSS--VDTRKWKNRR-PGVWRYKELLPKV 55
++C CG + A CG L+ YD + VD + RR +WRY ELLP
Sbjct: 10 LECVDCGESFAAAAATHRCPECGGILDPAYDLENLDVDAETFGARRFDSLWRYAELLPFT 69
Query: 56 K-KIVSLQEGGTPLIKAR-ISEQLNV-DVFIKDETRNPTGSFRDRLATVAISYGLPYAKN 112
+ VS+ EG T L+ ++++L V +++IKDE RNPTG+FRDR TVA++ + +
Sbjct: 70 RGSAVSMDEGATALVPCPGLADELGVGELYIKDEGRNPTGTFRDRGQTVAMTAAAQHGAS 129
Query: 113 GFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIE 172
+AS GNA S AY+ RA + V +P + + G + + DA
Sbjct: 130 EVALASTGNAGQSAGAYAGRAGLDAHVFLPSRSGFTTKAMVNVHGGDMNVVQGRIGDANA 189
Query: 173 YAKELAKLNG--LYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSI 227
++ +G Y++ EG+KT+ +E E+++ P HV+ PTG G L +
Sbjct: 190 AFEDAMDEHGEAWYSLQAFATPYRHEGKKTLLYETVEQLDWETPDHVVYPTGDGGGLVGM 249
Query: 228 YKGFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKG-----ECKETKALGLYVREPL 282
+K EL E+G ++P + A Q + C PI + + +T G+ + +P
Sbjct: 250 WKAATELRELGITADVPAMYAAQAEGCAPIVRAFQEGRDAHEPWDTPDTICGGIEIPDPA 309
Query: 283 MKPKVIRAIRESSGTAVLVSEEEI-EKGERLLANEGIFAELSSAVVMPALIKLVNDGFIE 341
V+ A+RES+G AV S+EEI E G + + G + A +L +
Sbjct: 310 GSRLVLEALRESNGGAVAASDEEILEAGTAVASRAGPEMGATCAAAAAGAWELADRDAFG 369
Query: 342 KGDRVVLVVTGSGLKTGE 359
+ D VV+V TG+ K +
Sbjct: 370 EDDTVVVVSTGTANKAAD 387
>emb|CAC11637.1| (AL445064) threonine synthase related protein [Thermoplasma
acidophilum]
Length = 334
Score = 129 bits (322), Expect = 5e-29
Identities = 101/349 (28%), Positives = 170/349 (47%), Gaps = 39/349 (11%)
Query: 21 GEELEITYDYSSVDTRKWKNRRPGVWRYKELLPKVKKIVSLQEGGTPLIKARISEQLNVD 80
GEEL S + R + R + ++ P +K ++SL E TP+ D
Sbjct: 9 GEELRCKSCGSPFEIRTGFHYRENI---EDNFPYIKNMISLGESETPIFHGD-------D 58
Query: 81 VFIKDETRNPTGSFRDR--------LATVAISYGLPYAKNGFIVASDGNAAASVAAYSAR 132
+K + PT S++DR +A + SYG+ S GNA AS+AAY +
Sbjct: 59 FDLKLDYMLPTFSYKDRGSRILISHIADIRDSYGITRISED----SSGNAGASIAAYGSA 114
Query: 133 AEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENI 192
A + +P V K Q+ A+GA +I+ S +D A+ + + PE
Sbjct: 115 AGLAVDIYVPETVAGRKFSQIQAYGANVIKVKGSREDVQSAAERSESFYASHVLRPEFR- 173
Query: 193 VGLEGQKTIAFELWEEIN--PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQ 250
+G +T+A+E++ + + P ++ +P +G+ L +Y G + L+E G + EMP ++AVQ
Sbjct: 174 ---DGIRTLAYEIYRQYDRMPRNIYIPVSAGTLLLGLYSGLRHLMESGEIAEMPAIVAVQ 230
Query: 251 TDRCNPIASEILGIKGECK---ETKALGLYVREPLMKPKVIRAIRESSGTAVLVSEEEIE 307
T+ +P+ ++I G+ + + A L R+P++ K+ + I E G + VS++EI
Sbjct: 231 TEAVSPLCAKINGLNYDPDNKLSSIADALVSRKPVLLQKMYQVISE-YGRCMTVSDDEII 289
Query: 308 KGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLK 356
+ LA +G E SSA V A K + + +LV+TGSGLK
Sbjct: 290 EARNRLAKKGFLVEYSSATVYAAFRK-------KPQENSLLVLTGSGLK 331
>gb|AAD55628.1|AC008017_1 (AC008017) Putative threonine synthase [Arabidopsis thaliana]
Length = 516
Score = 128 bits (319), Expect = 1e-28
Identities = 114/376 (30%), Positives = 183/376 (48%), Gaps = 45/376 (11%)
Query: 21 GEELEITYDYSSV------------DTRKWKNRRP---GVWRYKE-LLPKVKK--IVSLQ 62
G L++ +D++++ D+R K P GVW KE +LP++ IVS
Sbjct: 99 GALLDVQHDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAF 158
Query: 63 EGGTPLIKA-RISEQ---LNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKN-----G 113
EG + L A R +Q +N D+++K + TGSF+D +V +S K G
Sbjct: 159 EGNSNLFWAERFGKQYLQMN-DLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIG 217
Query: 114 FIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARIIRYGESVDDAIE 172
AS G+ +A+++AY A A V +P K+ +L+Q IA GA ++ D +
Sbjct: 218 VGCASTGDTSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMH 277
Query: 173 YAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSIYK 229
+E+ +Y + N + LEGQKT A E+ ++ N P VIVP G+ +Y+ YK
Sbjct: 278 LIREVTAELPIY-LANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYK 336
Query: 230 GFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKE---------TKALGLYVRE 280
GF E+G ++ +P+L+ Q NP+ L K KE T A + + +
Sbjct: 337 GFHMCKELGLVDRIPRLVCAQAANANPL---YLHYKSGFKEDFNPLKANTTFASAIQIGD 393
Query: 281 PLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFI 340
P+ + + A+++S+G +EEE+ L + G+F + V + AL+KL G I
Sbjct: 394 PVSIDRAVYALKKSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVI 453
Query: 341 EKGDRVVLVVTGSGLK 356
DR V+V T GLK
Sbjct: 454 GANDRTVVVSTAHGLK 469
>gb|AAF74984.1|AF082894_1 (AF082894) threonine synthase [Solanum tuberosum]
Length = 519
Score = 127 bits (317), Expect = 2e-28
Identities = 110/355 (30%), Positives = 173/355 (47%), Gaps = 30/355 (8%)
Query: 28 YDYSSVDTRKWKNRRP---GVWRYKE-LLPKVKK--IVSLQEGGTPLIKA-RISEQL--N 78
Y S D+R K P GVW KE +LP++ IVS EG + L A R +Q
Sbjct: 120 YWRSLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGM 179
Query: 79 VDVFIKDETRNPTGSFRDRLATVAISYGLPYAKN-----GFIVASDGNAAASVAAYSARA 133
D+++K + TGSF+D TV +S K G AS G+ +A+++AY A A
Sbjct: 180 TDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCASA 239
Query: 134 EKECFVVIP-RKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENI 192
V +P K+ +L+Q IA GA ++ D ++ +E+ +Y + N
Sbjct: 240 GIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIY-LANSLNS 298
Query: 193 VGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAV 249
+ LEGQKT A E+ ++ + P VIVP G+ +Y+ YKGF+ E+G ++ +P+L+
Sbjct: 299 LRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKELGLVDRIPRLVCA 358
Query: 250 QTDRCNPIASEILGIKGECKETK--------ALGLYVREPLMKPKVIRAIRESSGTAVLV 301
Q NP+ L K K+ K A + + +P+ + + A+++ +G
Sbjct: 359 QAANANPL---YLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFALQQCNGIVEEA 415
Query: 302 SEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLK 356
+EEE+ + G+F + V + AL KL N G I DR V+V T GLK
Sbjct: 416 TEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVSTAHGLK 470
>pir||S71362 threonine synthase (EC 4.2.99.2) precursor - Arabidopsis thaliana
(fragment) >gi|1448917|gb|AAB04607.1| (L41666) threonine
synthase [Arabidopsis thaliana]
Length = 525
Score = 127 bits (315), Expect = 3e-28
Identities = 108/349 (30%), Positives = 172/349 (48%), Gaps = 30/349 (8%)
Query: 34 DTRKWKNRRP---GVWRYKE-LLPKVKK--IVSLQEGGTPLIKA-RISEQL--NVDVFIK 84
D+R K+ P GVW KE +LP++ IVS EG + L A R +Q D+++K
Sbjct: 132 DSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVK 191
Query: 85 DETRNPTGSFRDRLATVAISYGLPYAKN-----GFIVASDGNAAASVAAYSARAEKECFV 139
+ TGSF+D TV +S K G AS G+ +A+++AY A A V
Sbjct: 192 HCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAYCASAGIPSIV 251
Query: 140 VIP-RKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQ 198
+P K+ +L+Q IA GA ++ D ++ +E+ +Y + N + LEGQ
Sbjct: 252 FLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAELPIY-LANSLNSLRLEGQ 310
Query: 199 KTIAFELWEEIN---PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCN 255
KT A E+ ++ + P VIVP G+ +Y+ YKGFK E+G ++ +P+++ Q N
Sbjct: 311 KTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGLVDRIPRMVCAQAANAN 370
Query: 256 PIASEILGIKGECKETK--------ALGLYVREPLMKPKVIRAIRESSGTAVLVSEEEIE 307
P+ L K K+ K A + + +P+ + + A+++ +G +EEE+
Sbjct: 371 PL---YLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKCNGIVEEATEEELM 427
Query: 308 KGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLK 356
+ G+F + V + AL KL N G I DR V+V T GLK
Sbjct: 428 DAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHGLK 476
>pir||T08545 threonine synthase (EC 4.2.99.2) precursor - Arabidopsis thaliana
>gi|4850369|dbj|BAA77707.1| (AB027151) threonine
synthase [Arabidopsis thaliana]
>gi|4914408|emb|CAB43659.1| (AL050352) threonine
synthase [Arabidopsis thaliana]
>gi|7269883|emb|CAB79742.1| (AL161575) threonine
synthase [Arabidopsis thaliana]
Length = 526
Score = 127 bits (315), Expect = 3e-28
Identities = 108/349 (30%), Positives = 172/349 (48%), Gaps = 30/349 (8%)
Query: 34 DTRKWKNRRP---GVWRYKE-LLPKVKK--IVSLQEGGTPLIKA-RISEQL--NVDVFIK 84
D+R K+ P GVW KE +LP++ IVS EG + L A R +Q D+++K
Sbjct: 133 DSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVK 192
Query: 85 DETRNPTGSFRDRLATVAISYGLPYAKN-----GFIVASDGNAAASVAAYSARAEKECFV 139
+ TGSF+D TV +S K G AS G+ +A+++AY A A V
Sbjct: 193 HCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDTSAALSAYCASAGIPSIV 252
Query: 140 VIP-RKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQ 198
+P K+ +L+Q IA GA ++ D ++ +E+ +Y + N + LEGQ
Sbjct: 253 FLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAELPIY-LANSLNSLRLEGQ 311
Query: 199 KTIAFELWEEIN---PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCN 255
KT A E+ ++ + P VIVP G+ +Y+ YKGFK E+G ++ +P+++ Q N
Sbjct: 312 KTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGLVDRIPRMVCAQAANAN 371
Query: 256 PIASEILGIKGECKETK--------ALGLYVREPLMKPKVIRAIRESSGTAVLVSEEEIE 307
P+ L K K+ K A + + +P+ + + A+++ +G +EEE+
Sbjct: 372 PL---YLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKCNGIVEEATEEELM 428
Query: 308 KGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLK 356
+ G+F + V + AL KL N G I DR V+V T GLK
Sbjct: 429 DAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHGLK 477
>gb|AAG20250.1| (AE005100) threonine synthase; ThrC3 [Halobacterium sp. NRC-1]
Length = 363
Score = 125 bits (311), Expect = 9e-28
Identities = 106/369 (28%), Positives = 157/369 (41%), Gaps = 26/369 (7%)
Query: 3 CPKCGREYKA--EIPPFCIC-------GEELEITYDYSSVDTRKWKNRRPGVWRYKELLP 53
C CG Y+ E P C E T D ++VD G+W + E LP
Sbjct: 4 CRSCGATYETGPEEPWRCRACEHAVDFAERPRATGDPTAVDPTA------GLWAFSEFLP 57
Query: 54 -KVKKIVSLQEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKN 112
VSL EG TPL+ A D K + P+GS++DR A V ++ +
Sbjct: 58 VAADDAVSLGEGWTPLVSAS-----EWDAEFKLDYVCPSGSYKDRGAAVLMTRAAALGVD 112
Query: 113 GFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIE 172
+ S GNA A+VA Y+ARA + + +P KL M A GA + E
Sbjct: 113 RVVEDSSGNAGAAVAQYAARAGIDAEIYVPADAPAAKLDAMEAAGATTVPVAGGRRAVTE 172
Query: 173 YAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEIN---PTHVIVPTGSGSYLYSIYK 229
A + + + + G T+A+E+ + P V+VP G G+ L Y+
Sbjct: 173 ACVSEAVGSDAWYASHAWSPSFYAGTATVAYEIAAQRGWRAPDAVVVPVGHGTLLLGAYR 232
Query: 230 GFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKG--ECKETKALGLYVREPLMKPKV 287
GF+ L G +E MP+L+ VQ P+A+ + G + A G+ +R P +V
Sbjct: 233 GFRALAAGGWIESMPRLLGVQAAGVEPVAAAVNGSASDEDAVNEAADGIQIRSPARLDQV 292
Query: 288 IRAIRESSGTAVLVSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVV 347
A+ + G V E L G++ E +SAV + L G + D VV
Sbjct: 293 AAAVAATDGDVVACDESATTDALASLHEAGLYVEPTSAVAVAGLAAYRARGVVGDSDDVV 352
Query: 348 LVVTGSGLK 356
+ +TGSGLK
Sbjct: 353 VALTGSGLK 361
>emb|CAC11175.1| (AL445063) threonine synthase related protein [Thermoplasma
acidophilum]
Length = 316
Score = 122 bits (304), Expect = 6e-27
Identities = 91/310 (29%), Positives = 165/310 (52%), Gaps = 30/310 (9%)
Query: 64 GGTPLIKA-RISEQLNVD-VFIKDETRNPTGSFRDRLATVAISYGLPYAKNGFIVASDGN 121
G TP IKA + +L+++ +F+K E N TG+ +DR++ + + NG V + GN
Sbjct: 13 GRTPTIKAYALGNKLHLNNLFLKFEGANATGTQKDRISEAHVINAMRGGYNGIAVGTCGN 72
Query: 122 AAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLN 181
AS+A ++ + + ++ IPR + M + A I+ Y ++ +E+ ++ +K N
Sbjct: 73 YGASIAYFAHQYGVKSYIGIPRSYSHDRTDFMQSEQAEILYYDGKYEETVEFIRDFSKDN 132
Query: 182 GLYNVTPEENIVGLEGQ--KTIAFELWEEI--NPTHVIVPTGSGSYLYSIYKGFKELIEI 237
GLY+ +P ++ TIAFE++E + P++VIVP G+G+ L IY GF+ + +
Sbjct: 133 GLYDASPGSANSSIDKMMYATIAFEIYEALLTVPSYVIVPVGNGTTLAGIYHGFRRMYDE 192
Query: 238 GALEEMPKLIAVQTDRCNPIA-------SEILGIK-GECKETKALGLYVREPLMKPK--- 286
G ++ +P+++ V T NPI S ++ + E +ET + EPL+ +
Sbjct: 193 GVIDHLPRMVGVSTSGGNPIVDSWRHGYSRVVDLNPEEIRETS-----INEPLVSYRSFD 247
Query: 287 ---VIRAIRESSGTAVLVSEEEIEKGERLL-ANEGIFAELSSAVVMPALIKLVNDGFIEK 342
+ AIRE+ G A+ VS+EE+ L+ + EG+ +SA + A+ L +++
Sbjct: 248 GQDALSAIRETHGRAMYVSDEEMVYYSNLIYSAEGLNPLPASASAVAAIGHLK----VKQ 303
Query: 343 GDRVVLVVTG 352
+ VV V+TG
Sbjct: 304 DETVVSVITG 313
>pir||H72628 probable threonine synthase (EC 4.2.99.2) APE1488 [similarity] -
Aeropyrum pernix (strain K1) >gi|5105172|dbj|BAA80486.1|
(AP000061) 345aa long hypothetical threonine synthase
[Aeropyrum pernix]
Length = 345
Score = 109 bits (270), Expect = 6e-23
Identities = 98/308 (31%), Positives = 145/308 (46%), Gaps = 22/308 (7%)
Query: 52 LPKVKKIVSLQEGGTPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK 111
LP++ + L EG TPL++A V+ K E NP+GSF+DR ++
Sbjct: 44 LPRLPPV--LGEGSTPLVEA------GEGVYAKLEYLNPSGSFKDRGVGYSLRMASLLGY 95
Query: 112 NGFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAI 171
+ +V S GN S A YSAR + V +PR GK + A GA ++ ES ++A
Sbjct: 96 DCVVVDSSGNTGLSTALYSARLGLKARVYVPRGASPGKKALIRATGAELVE-AESREEAA 154
Query: 172 EYAKELAKLNGLYNVTPEENIVGLEGQKTIAFEL-WEEINPTHVIVPTGSGSYLYSIYKG 230
A+ A+ ++V N + +EG K++ EL E T V+ P SG+ L IY+G
Sbjct: 155 RLAE--AEAEKCFHVAHPTNPLFIEGVKSLGRELAGEAAGDTDVLAPVSSGTLLLGIYRG 212
Query: 231 FKELIEIGALEEMPKLIAVQTDRCNPIASEI--LGIKGECKETKALGLYVREPLMKPKVI 288
+E E G +L+AVQ+ + + LG G + L + P ++
Sbjct: 213 LEE--ERGRF----RLVAVQSPAAYSLKGLVPELGWVGGVEGRLLDALLLARPPRLEEMA 266
Query: 289 RAIRESSGTAVLVSEEEIEKGERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVL 348
RA+RES G V+V + I R G E SSA V A L ++ R +L
Sbjct: 267 RAVRESGGGVVVVGDGAIPGAVREAVQRGFLVEPSSAAVFAAHEVLREKKWL--SGRSLL 324
Query: 349 VVTGSGLK 356
V+TGSGLK
Sbjct: 325 VLTGSGLK 332
>gb|AAB40316.1| (U46200) threonine synthase [Cloning vector pDG1663]
>gi|1185595|gb|AAB40324.1| (U46201) threonine synthase
[Cloning vector pDG1664] >gi|1185603|gb|AAB40331.1|
(U46202) threonine synthase [Cloning vector pDG1729]
>gi|1185612|gb|AAB40339.1| (U46203) threonine synthase
[Cloning vector pDG1731]
Length = 167
Score = 108 bits (266), Expect = 2e-22
Identities = 63/163 (38%), Positives = 97/163 (58%), Gaps = 5/163 (3%)
Query: 45 VWRYKELLPKVKKI--VSLQEGGTPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATV 101
+ +YKE LP + ++L EG TPLI ++SEQL +++ +K E NPTGSF+DR +
Sbjct: 6 IHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIELHVKTEGVNPTGSFKDRGMVM 65
Query: 102 AISYGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPR-KVDKGKLIQMIAFGARI 160
A++ + + AS GN +A+ AAY+ARA +C V+IP K+ GKL Q + +GA I
Sbjct: 66 AVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVIIPNGKIAFGKLAQAVMYGAEI 125
Query: 161 IRYGESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQKTIAF 203
I + DDA++ + + + + + V N +EGQKT AF
Sbjct: 126 IAIDGNFDDALKIVRSICEKSPIALVN-SVNPYRIEGQKTAAF 167
>gb|AAK40587.1| Threonine dehydratase catabolic (threonine deaminase) (tdcB)
[Sulfolobus solfataricus]
Length = 405
Score = 87.4 bits (213), Expect = 3e-16
Identities = 90/309 (29%), Positives = 144/309 (46%), Gaps = 22/309 (7%)
Query: 54 KVKKIVSLQEGGTPL-IKARISEQLNVDVFIKDETRNPTGSFRDRLA-TVAISYGLPYAK 111
K++K V + TP+ S +N V++K E TGSF+ R A +S K
Sbjct: 16 KIEKYVHI----TPIDYSTTFSRIINAKVYLKLENLQKTGSFKVRGAFNKLLSLKEEEKK 71
Query: 112 NGFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAI 171
NG I S GN A VA ++ + +V+P K + ++GA ++ YG+ + +++
Sbjct: 72 NGVIAVSAGNHAQGVAYAASTLNIKSTIVMPETAPASKYLATKSYGAEVVLYGKYLHESM 131
Query: 172 EYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEINPTHVIVPTGSGSYLYSIYKGF 231
+ A+EL + GL V P ++ + GQ TI EL+ +I P +VI+P G G + I
Sbjct: 132 KKAEELIQNTGLIFVHPYSDLDVITGQGTIGLELY-DIEPDYVIIPIGGGGLISGISIAL 190
Query: 232 KELIEIGALEEMPKLIAVQTDRCNPI-ASEILG--IKGECKETKALGLYVREPLMKPKVI 288
K K+I VQ+ + S+ LG ++ E + A G+ V+ P
Sbjct: 191 KYRF------PNVKIIGVQSSSSPSMKVSKDLGRLVEIEPSYSIADGILVKSP--SELTF 242
Query: 289 RAIRESSGTAVLVSEEEI-EKGERLLANEGIFAELSSAVVMPALIK-LVNDGFIEKGDRV 346
I E VLV +EEI E LL AE + A + +LI V I+K +V
Sbjct: 243 SIINELVDDIVLVDDEEIAEAIVLLLERSKTLAEGAGAAALASLISGKVKVNGIDK--KV 300
Query: 347 VLVVTGSGL 355
+ +V+G +
Sbjct: 301 ISLVSGGNI 309
>sp|P46493|THD1_HAEIN THREONINE DEHYDRATASE BIOSYNTHETIC (THREONINE DEAMINASE)
>gi|1573745|gb|AAC22398.1| (U32757) threonine deaminase
(ilvA) [Haemophilus influenzae Rd]
Length = 513
Score = 84.3 bits (205), Expect = 3e-15
Identities = 83/323 (25%), Positives = 146/323 (44%), Gaps = 16/323 (4%)
Query: 40 NRRPGVWRYKELLPKVKKIVSLQEGGTPLIK-ARISEQLNVDVFIKDETRNPTGSFRDRL 98
N +P Y + K+ V TPL K ++SE+L+ +++IK E R P SF+ R
Sbjct: 7 NPQPSQSDYINAIVKLGSRVYEAAQVTPLQKMGKLSERLHNNIWIKREDRQPVNSFKLRG 66
Query: 99 ATVAIS-YGLPYAKNGFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFG 157
A IS G I AS GN A VA + + + +V+P+ K+ + FG
Sbjct: 67 AYAMISSLSAEQKAAGVIAASAGNHAQGVALSAKQLGLKALIVMPQNTPSIKVDAVRGFG 126
Query: 158 ARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEI-NPTHVIV 216
++ +G + D+A A EL+K + + P ++ + + GQ T+A E+ +++ + +V V
Sbjct: 127 GEVLLHGANFDEAKAKAIELSKEKNMTFIPPFDHPLVIAGQGTLAMEMLQQVADLDYVFV 186
Query: 217 PTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIASEILGIKGECKETKALGL 276
G G G + I + MP++ + + + + KGE + +GL
Sbjct: 187 QVGGG--------GLAAGVAILLKQFMPEIKIIGVESKDSACLKAALDKGEPTDLTHIGL 238
Query: 277 YVREPLMK---PKVIRAIRESSGTAVLVSEEEIEKGER-LLANEGIFAELSSAVVMPALI 332
+ +K + R ++ VLV +E+ + L N AE S A+ + L
Sbjct: 239 FADGVAVKRIGDETFRLCQQYLDDMVLVDSDEVCAAMKDLFENVRAVAEPSGALGLAGLK 298
Query: 333 KLVNDGFIEKGDRVVLVVTGSGL 355
K V IE G + +++G+ L
Sbjct: 299 KYVKQNHIE-GKNMAAILSGANL 320
>pir||D72386 threonine dehydratase (EC 4.2.1.16) TM0356 [similarity] -
Thermotoga maritima (strain MSB8)
>gi|4980859|gb|AAD35443.1|AE001716_6 (AE001716)
threonine dehydratase catabolic [Thermotoga maritima]
Length = 401
Score = 80.8 bits (196), Expect = 3e-14
Identities = 76/289 (26%), Positives = 132/289 (45%), Gaps = 18/289 (6%)
Query: 73 ISEQLNVDVFIKDETRNPTGSFRDRLATVAISY-GLPYAKNGFIVASDGNAAASVAAYSA 131
+SE ++++K E TGSF+ R A I++ K G + AS GN A VA +
Sbjct: 29 LSEVTGGEIYLKMENLQKTGSFKIRGAYNKIAHLSEEERKRGVVAASAGNHAQGVALAAQ 88
Query: 132 RAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEEN 191
+V+PR K+ + GA++I G D+A E A + + G V P +
Sbjct: 89 IFGIPATIVMPRYAPLSKITKTRNLGAQVILEGNIFDEAYEAALRIQEKTGAVFVHPFND 148
Query: 192 IVGLEGQKTIAFELWEEINPTH-VIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQ 250
+ GQ TI E+ E++ V+VP G G + + + I ++ K+I VQ
Sbjct: 149 PHVIAGQGTIGLEIMEDLPDVEVVVVPVGGGGLISGV------SVAIKSMNPEVKVIGVQ 202
Query: 251 TDRCNPIASEI---LGIKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVLVSEEEIE 307
T+ + + + + E K T A G+ V++P +++ V V+EEEI
Sbjct: 203 TENMPSMIASLRRGRAERVEGKPTLADGIAVKKP--GDLTFELVKKYVDEMVAVNEEEIA 260
Query: 308 KGERLLANEG-IFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGL 355
L + + AE + AV + A++ ++ KG +V +V++G +
Sbjct: 261 DAILFLLEQAKVVAEGAGAVGVAAVLNKLD----VKGKKVAIVISGGNI 305
>sp|P05792|THD2_ECOLI THREONINE DEHYDRATASE CATABOLIC (THREONINE DEAMINASE)
>gi|68320|pir||DWECTD threonine dehydratase (EC
4.2.1.16), biodegradative [validated] - Escherichia coli
>gi|43043|emb|CAA32593.1| (X14430) tdcB gene product (AA
1 - 329) [Escherichia coli] >gi|147923|gb|AAA24660.1|
(M21312) threonine dehydratase 2 (EC 4.2.1.16)
[Escherichia coli] >gi|606058|gb|AAA57921.1| (U18997)
catabolic threonine dehydratase [Escherichia coli]
>gi|1789505|gb|AAC76152.1| (AE000393) threonine
dehydratase, catabolic [Escherichia coli K12]
>gi|228767|prf||1811219A Thr deaminase [Escherichia
coli]
Length = 329
Score = 79.2 bits (192), Expect = 9e-14
Identities = 75/288 (26%), Positives = 129/288 (44%), Gaps = 18/288 (6%)
Query: 74 SEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK-NGFIVASDGNAAASVAAYSAR 132
SE+ ++F+K E TGSF+ R A +S K G + S GN A V+ A
Sbjct: 37 SERCKGEIFLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAM 96
Query: 133 AEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENI 192
+ VV+P+ K K+ + A ++ +G++ +D I E+ ++ G + P ++
Sbjct: 97 LGIDGKVVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDP 156
Query: 193 VGLEGQKTIAFELWEEI-NPTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQT 251
+ GQ TI E+ E++ + +VIVP G G + I + I ++ ++I VQ+
Sbjct: 157 KVIAGQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGI------AVAIKSINPTIRVIGVQS 210
Query: 252 DRCNPIASEILGIKGECKE-----TKALGLYVREPLMKPKVIRAIRESSGTAVLVSEEEI 306
+ + +A+ GE T A G V P +RE VLVSE+EI
Sbjct: 211 ENVHGMAASF--HSGEITTHRTTGTLADGCDVSRP--GNLTYEIVRELVDDIVLVSEDEI 266
Query: 307 EKGE-RLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGS 353
L+ + E + A+ AL+ D +I+ V ++ G+
Sbjct: 267 RNSMIALIQRNKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGN 314
>gb|AAF33479.1| (AF233324) S. typhimurium threonine deaminase (ILVA) (SP:P20506);
contains similarity to Pfam families PF00291
(Pyridoxal-phosphate dependent enzyme, score=467.9,
E=8.4e-137, N=1) and PF00585 (C-terminal domain of
Threonine dehydratase, score=329.2, E=>
Length = 514
Score = 76.1 bits (184), Expect = 7e-13
Identities = 81/299 (27%), Positives = 140/299 (46%), Gaps = 20/299 (6%)
Query: 66 TPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK--NGFIVASDGNA 122
TPL K ++S +L+ + +K E R P SF+ R A A+ GL + +G I AS GN
Sbjct: 32 TPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGA-YAMMAGLTEEQKAHGVITASAGNH 90
Query: 123 AASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNG 182
A VA SAR + +V+P+ K+ + FG ++ +G + D+A A ELA+ G
Sbjct: 91 AQGVAFSSARLGVKSLIVMPKATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELAQQQG 150
Query: 183 LYNVTPEENIVGLEGQKTIAFE-LWEEINPTHVIVPTGSGSYLYSIYKGFKEL---IEIG 238
V P ++ + + GQ T+A E L ++ + V VP G G + K+L I++
Sbjct: 151 FTWVPPFDHPMVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVI 210
Query: 239 ALE-EMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGT 297
A+E E + + +P+ +G+ E K +G + R +E
Sbjct: 211 AVEAEDSACLKAALEAGHPVDLPRVGLFAEGVAVKRIG---------DETFRLCQEYLDD 261
Query: 298 AVLVSEEEIEKGER-LLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGL 355
+ V + I + L + AE S A+ + + K + I +G+R+ V++G+ +
Sbjct: 262 IITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIAQHNI-RGERLAHVLSGANV 319
>gb|AAA24014.1| (K03503) threonine dehydratase [Escherichia coli]
Length = 514
Score = 75.3 bits (182), Expect = 1e-12
Identities = 80/299 (26%), Positives = 140/299 (46%), Gaps = 20/299 (6%)
Query: 66 TPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK--NGFIVASDGNA 122
TPL K ++S +L+ + +K E R P SF+ R A A+ GL + +G I AS GN
Sbjct: 32 TPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGA-YAMMAGLTEEQKAHGVITASAGNH 90
Query: 123 AASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNG 182
A VA SAR + +V+P K+ ++ FG ++ +G + D+A A EL++ G
Sbjct: 91 AQGVAFSSARLGVKALIVMPTATADIKVDRLRGFGGEVLLHGANFDEAKRKAIELSQQQG 150
Query: 183 LYNVTPEENIVGLEGQKTIAFE-LWEEINPTHVIVPTGSGSYLYSIYKGFKEL---IEIG 238
V P ++ + + GQ T+A E L ++ + V VP G G + K+L I++
Sbjct: 151 FTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAACVAVLIKQLMPQIKVI 210
Query: 239 ALE-EMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGT 297
A+E E + D +P+ +G+ E K +G + R +E
Sbjct: 211 AVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIG---------DETFRLCQEYLDD 261
Query: 298 AVLVSEEEIEKGER-LLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGL 355
+ V + I + L + AE S A+ + + K + I +G+R+ +++G+ +
Sbjct: 262 IITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNI-RGERLAHILSGANV 319
>gb|AAA67575.1| (M87049) threonine deaminase [Escherichia coli]
Length = 514
Score = 75.3 bits (182), Expect = 1e-12
Identities = 80/299 (26%), Positives = 139/299 (45%), Gaps = 20/299 (6%)
Query: 66 TPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK--NGFIVASDGNA 122
TPL K ++S +L+ + +K E R P SF+ R A A+ GL + +G I AS GN
Sbjct: 32 TPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGA-YAMMAGLTEEQKAHGVITASAGNH 90
Query: 123 AASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNG 182
A VA SAR + +V+P K+ + FG ++ +G + D+A A EL++ G
Sbjct: 91 AQGVAFSSARLGVKALIVMPTATADIKVDAVXGFGGEVLLHGANFDEAKRKAIELSQQQG 150
Query: 183 LYNVTPEENIVGLEGQKTIAFE-LWEEINPTHVIVPTGSGSYLYSIYKGFKEL---IEIG 238
V P ++ + + GQ T+A E L ++ + V VP G G + K+L I++
Sbjct: 151 FTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVI 210
Query: 239 ALE-EMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGT 297
A+E E + D +P+ +G+ E K +G + R +E
Sbjct: 211 AVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIG---------DETFRLCQEYLDD 261
Query: 298 AVLVSEEEIEKGER-LLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGL 355
+ V + I + L + AE S A+ + + K + I +G+R+ +++G+ +
Sbjct: 262 IITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNI-RGERLAHILSGANV 319
>gb|AAF54364.1| (AE003682) CG8129 gene product [alt 1] [Drosophila melanogaster]
Length = 469
Score = 75.3 bits (182), Expect = 1e-12
Identities = 80/303 (26%), Positives = 133/303 (43%), Gaps = 16/303 (5%)
Query: 66 TPLIKARISEQLNVDVFIKDETRNPTGSFRDRLATVAI-SYGLPYAKNGFIVASDGNAAA 124
TP K+ S+ +++++K + TGSF++R A A+ S + G I AS GN A
Sbjct: 78 TPCPKSTSSDLYGMELYLKKDFLQYTGSFKERGARYALLSLTEEQKRTGVISASLGNHAQ 137
Query: 125 SVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLY 184
++ + + VV+P+ K+ + + AR+I G + +A A +++ GL
Sbjct: 138 ALCYHGWKLNIPVTVVMPKAAPIMKIQKCRNYKARVIVDGNDMGEAKSLAMRMSREEGLL 197
Query: 185 NVTPEENIVGLEGQKTIAFELWEEI-NPTHVIVPTGSGSYLYSIYKGFKELIEIGALEEM 243
V ++ + GQ TI E+ E++ P V+VP G G + I K AL
Sbjct: 198 YVNGYDHPHIMAGQGTIGLEILEQVPEPDAVVVPVGGGGLIAGIATAVK------ALSPK 251
Query: 244 PKLIAVQTDRCNPIASEILG---IKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVL 300
K+I V++++C + I K T A GL V P + V+
Sbjct: 252 TKIIGVESEKCASFTRAMENDGPIHTPIKNTLADGLAV--PKVGYNAYATAMPLIDRMVV 309
Query: 301 VSEEEIEKG-ERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLKTGE 359
V EE I RL+ E E + + A++ D KG +VV+++ G + T
Sbjct: 310 VKEEWIAVAILRLVEEEKCVVEGAGGAGLAAILAGHLDEL--KGKKVVVLLCGGNIDTTV 367
Query: 360 GGR 362
GR
Sbjct: 368 FGR 370
>pir||T23166 hypothetical protein K01C8.1 - Caenorhabditis elegans
>gi|3878113|emb|CAA88854.1| (Z49068) contains similarity
to Pfam domain: PF00291 (Pyridoxal-phosphate dependent
enzyme), Score=292.1, E-value=2.3e-84, N=1; PF01842 (ACT
domain), Score=38.9, E-value=3.8e-08, N=1~cDNA EST
EMBL:M89183 comes from this gene~cDNA EST yk195g4.5
comes fr>
Length = 512
Score = 74.5 bits (180), Expect = 2e-12
Identities = 84/314 (26%), Positives = 143/314 (44%), Gaps = 24/314 (7%)
Query: 66 TPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAIS-YGLPYAKNGFIVASDGNAA 123
TP +++ ++S + +D++ K E TGSF++R A A+S + K G I AS GN A
Sbjct: 118 TPCVRSLQLSSKCEMDLYFKKEYLQVTGSFKERGARYALSKMAEQFKKAGVIAASAGNHA 177
Query: 124 ASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGL 183
+++ + + VV+P K+ + GA +I GES+ A ++A AK N L
Sbjct: 178 LALSYHGQQMGIPVTVVMPVIAPLMKIQFCRSLGATVILKGESIAVAKDFALRHAKENHL 237
Query: 184 YNVTPEENIVGLEGQKTIAFELWEEI-NPTHVIVPTGSGSYLYSIYKGFKEL-------I 235
+ + I L GQ TI E+ +++ + ++VP G G + I K L +
Sbjct: 238 KYINGYDAIDILAGQGTIGLEILDQVPDVDTILVPVGGGGLIAGIATAVKTLKPDVHIYV 297
Query: 236 EIGALEEMPKLIAVQTDRCNPIASEILG----IKGECKETKALGLYVREPLMKPKVIRAI 291
+ K+ ++++ C P +E I + K + A GL V P + +
Sbjct: 298 SSFLIIANFKVTGIESETC-PSFTEAYEAGHIITSQAKASLADGLAV--PTVGGNSLETA 354
Query: 292 RESSGTAVLVSEEEIEKG-ERLLANEGIFAELSSAVVMPALIKLVNDGFIE--KGDRVVL 348
+ + V EE I RLL E E AV + A++ +G + KG +VV
Sbjct: 355 KGLVDKVITVKEESIALSILRLLEVEKAVVEGGGAVGLAAIL----EGKVPELKGKKVVS 410
Query: 349 VVTGSGLKTGEGGR 362
+++G + T GR
Sbjct: 411 ILSGGNIDTTVLGR 424
>sp|P04968|THD1_ECOLI THREONINE DEHYDRATASE BIOSYNTHETIC (THREONINE DEAMINASE)
>gi|68318|pir||DWECTS threonine dehydratase (EC
4.2.1.16), biosynthetic - Escherichia coli
>gi|3891839|pdb|1TDJ| Threonine Deaminase
(Biosynthetic) From E. Coli >gi|146462|gb|AAB59054.1|
(M10313) threonine deaminase [Escherichia coli]
>gi|288533|emb|CAA28577.1| (X04890) threonine deaminase
[Escherichia coli] >gi|1790207|gb|AAC77492.1| (AE000453)
threonine deaminase (dehydratase) [Escherichia coli K12]
>gi|225743|prf||1312306B gene ilvGMEDA cluster
[Escherichia coli]
Length = 514
Score = 74.1 bits (179), Expect = 3e-12
Identities = 80/299 (26%), Positives = 139/299 (45%), Gaps = 20/299 (6%)
Query: 66 TPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK--NGFIVASDGNA 122
TPL K ++S +L+ + +K E R P SF+ R A A+ GL + +G I AS GN
Sbjct: 32 TPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGA-YAMMAGLTEEQKAHGVITASAGNH 90
Query: 123 AASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNG 182
A VA SAR + +V+P K+ + FG ++ +G + D+A A EL++ G
Sbjct: 91 AQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQG 150
Query: 183 LYNVTPEENIVGLEGQKTIAFE-LWEEINPTHVIVPTGSGSYLYSIYKGFKEL---IEIG 238
V P ++ + + GQ T+A E L ++ + V VP G G + K+L I++
Sbjct: 151 FTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVI 210
Query: 239 ALE-EMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGT 297
A+E E + D +P+ +G+ E K +G + R +E
Sbjct: 211 AVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIG---------DETFRLCQEYLDD 261
Query: 298 AVLVSEEEIEKGER-LLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGL 355
+ V + I + L + AE S A+ + + K + I +G+R+ +++G+ +
Sbjct: 262 IITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNI-RGERLAHILSGANV 319
>sp|P20506|THD1_SALTY THREONINE DEHYDRATASE BIOSYNTHETIC (THREONINE DEAMINASE)
>gi|68319|pir||DWEBTT threonine dehydratase (EC
4.2.1.16), biosynthetic - Salmonella typhimurium
>gi|514966|gb|AAA27150.1| (M26670) threonine deaminase
[Salmonella typhimurium]
Length = 514
Score = 73.7 bits (178), Expect = 4e-12
Identities = 80/299 (26%), Positives = 139/299 (45%), Gaps = 20/299 (6%)
Query: 66 TPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK--NGFIVASDGNA 122
TPL K ++S +L+ + +K E R P SF+ R A A+ GL + +G I AS GN
Sbjct: 32 TPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGA-YAMMTGLTEEQKAHGVITASAGNH 90
Query: 123 AASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNG 182
A VA SAR + +V+P+ K+ + G ++ +G + D+A A ELA+ G
Sbjct: 91 AQGVAFSSARLGVKSLIVMPKATADIKVDAVRGLGGEVLLHGANFDEAKAKAIELAQQQG 150
Query: 183 LYNVTPEENIVGLEGQKTIAFE-LWEEINPTHVIVPTGSGSYLYSIYKGFKEL---IEIG 238
V P ++ + + GQ T+A E L ++ + V VP G G + K+L I++
Sbjct: 151 FTWVPPFDHPMVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVI 210
Query: 239 ALE-EMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGT 297
A+E E + + +P+ +G+ E K +G + R +E
Sbjct: 211 AVEAEDSACLKAALEAGHPVDLPRVGLFAEGVAVKRIG---------DETFRLCQEYLDD 261
Query: 298 AVLVSEEEIEKGER-LLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGL 355
+ V + I + L + AE S A+ + + K + I +G+R+ V++G+ +
Sbjct: 262 IITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIAQHNI-RGERLAHVLSGANV 319
>gb|AAA24024.1| (M32253) ilvA [Escherichia coli]
Length = 514
Score = 73.7 bits (178), Expect = 4e-12
Identities = 80/299 (26%), Positives = 139/299 (45%), Gaps = 20/299 (6%)
Query: 66 TPLIKA-RISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAK--NGFIVASDGNA 122
TPL K ++S +L+ + +K E R P SF+ R A A+ GL + +G I AS GN
Sbjct: 32 TPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGA-YAMMAGLTEEQKAHGVITASAGNH 90
Query: 123 AASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNG 182
A VA SAR + +V+P K+ + FG ++ +G + D+A A EL++ G
Sbjct: 91 AQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQG 150
Query: 183 LYNVTPEENIVGLEGQKTIAFE-LWEEINPTHVIVPTGSGSYLYSIYKGFKEL---IEIG 238
V P ++ + + GQ T+A E L ++ + V VP G G + K+L I++
Sbjct: 151 FTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVI 210
Query: 239 ALE-EMPKLIAVQTDRCNPIASEILGIKGECKETKALGLYVREPLMKPKVIRAIRESSGT 297
A+E E + D +P+ +G+ E K +G + R +E
Sbjct: 211 AVEAEDSACLKAALDAGHPVDLPRVGLFAEGVGVKRIG---------DETFRLCQEYLDD 261
Query: 298 AVLVSEEEIEKGER-LLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGL 355
+ V + I + L + AE S A+ + + K + I +G+R+ +++G+ +
Sbjct: 262 IITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNI-RGERLAHILSGANV 319
>gb|AAG20244.1| (AE005099) threonine dehydratase; IluA [Halobacterium sp. NRC-1]
Length = 495
Score = 72.2 bits (174), Expect = 1e-11
Identities = 80/291 (27%), Positives = 130/291 (44%), Gaps = 22/291 (7%)
Query: 74 SEQLNVDVFIKDETRNPTGSFRDRLATVAI-SYGLPYAKNGFIVASDGNAAASVAAYSAR 132
S+ DV K E TGSF+ R AT I + G + AS GN A VA ++R
Sbjct: 115 SDLTGADVRPKLECFQRTGSFKIRGATNRIRTLSADQQDAGVVTASAGNHAQGVALAASR 174
Query: 133 AEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEENI 192
+ + VV+P K+ ++GA ++ +G DDA +A EL G V ++
Sbjct: 175 SGVDSKVVMPESAPISKIKATKSYGAEVVLHGADYDDAQAHAHELEAAEGRTYVHAFDDE 234
Query: 193 VGLEGQKTIAFELWEEINPT--HVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQ 250
+ GQ T+ E+ + PT V+VP G G + + K G L+++ ++I VQ
Sbjct: 235 YIMAGQGTLGLEIAADC-PTVDTVVVPIGGGGLISGVATALK-----GELDDV-RVIGVQ 287
Query: 251 TDRCNPIASEILGIKGECK-----ETKALGLYVREPLMKPKVIRAIRESSGTAVLVSEEE 305
+ + +A + KG+ + +T A G+ VR+ + + I+E V VS++E
Sbjct: 288 AEGASTVARSL--DKGQPQAVDHVDTIADGIAVRQ--VGAQTFPVIQEHVDEVVTVSDDE 343
Query: 306 IEKGERLLANEGIFAELSSAVVMPALIKLVNDGF-IEKGDRVVLVVTGSGL 355
I LL G L AL ++ D F G+ +V + G +
Sbjct: 344 IATALVLLLERG--KTLVEGAGATALAAVLEDKFEYADGETIVPALCGGNI 392
>gb|AAF59508.1| (AC024805) contains similarity to SW:THD2_ECOLI [Caenorhabditis
elegans]
Length = 368
Score = 71.4 bits (172), Expect = 2e-11
Identities = 76/298 (25%), Positives = 134/298 (44%), Gaps = 20/298 (6%)
Query: 73 ISEQLNVDVFIKDETRNPTGSFRDRLATVAISYGLPYAKN--GFIVASDGNAAASVAAYS 130
+S+ ++++++K E TGSF++R A A+ LP K G AS GN A +++ +
Sbjct: 69 LSKLFDMNIYLKMEVNQDTGSFKERGARYALQ-NLPEEKKKAGVYAASAGNHALALSLHG 127
Query: 131 ARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELA-KLNGLYNVTPE 189
+ E VV+P+ K+ + GA I+ GE + ++ E A +A NG Y +
Sbjct: 128 KQLGVEVNVVMPKIAPLMKINRCQDLGANILVQGEDIAESREIALRMAHDSNGTY-INGY 186
Query: 190 ENIVGLEGQKTIAFELWEEIN-PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIA 248
++ L G T E+ E+I P ++VP G G + + K AL ++I
Sbjct: 187 DHYDILAGAGTTGIEILEQIQMPDAILVPVGGGGLVAGVATAVK------ALSPTTEVIG 240
Query: 249 VQTDRCNPIASEILG---IKGECKETKALGLYVREPLMKPKVIRAIRESSGTAVLVSEEE 305
V ++ C I + + + T A GL V P ++ + V E +
Sbjct: 241 VVSETCQAIVKSLQAGHPVYTPTRPTLADGLAV--PNAGVNAFASMIGKVDRVISVPEND 298
Query: 306 IEKG-ERLLANEGIFAELSSAVVMPALIKLVNDGFIEKGDRVVLVVTGSGLKTGEGGR 362
I RL+ NE + E + A+ + A++ + KG VV +++G + + GR
Sbjct: 299 IAVAILRLIENEKVVCEGAGAIGIGAILSGKLNHL--KGKTVVSILSGGNIDSTSLGR 354
>sp|P25306|THD1_LYCES THREONINE DEHYDRATASE BIOSYNTHETIC PRECURSOR (THREONINE DEAMINASE)
>gi|100257|pir||A38628 threonine dehydratase (EC
4.2.1.16) - tomato >gi|170460|gb|AAA68097.1| (M61915)
threonine deaminase [Lycopersicon esculentum]
Length = 595
Score = 71.0 bits (171), Expect = 2e-11
Identities = 48/165 (29%), Positives = 84/165 (50%), Gaps = 2/165 (1%)
Query: 72 RISEQLNVDVFIKDETRNPTGSFRDRLATVAIS-YGLPYAKNGFIVASDGNAAASVAAYS 130
++S++L V+ +IK E + SF+ R A +S G I AS GN A VA
Sbjct: 120 KLSDRLGVNFYIKREDKQRVFSFKLRGAYNMMSNLSREELDKGVITASAGNHAQGVALAG 179
Query: 131 ARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEE 190
R +V+P + K+ + A G ++ YG++ D+A +A EL++ +GL + P +
Sbjct: 180 QRLNCVAKIVMPTTTPQIKIDAVRALGGDVVLYGKTFDEAQTHALELSEKDGLKYIPPFD 239
Query: 191 NIVGLEGQKTIAFELWEEINPTH-VIVPTGSGSYLYSIYKGFKEL 234
+ ++GQ TI E+ ++ H V +P G G + + FK++
Sbjct: 240 DPGVIKGQGTIGTEINRQLKDIHAVFIPVGGGGLIAGVATFFKQI 284
>gb|AAA34171.1| (M61914) threonine deaminase [Lycopersicon esculentum]
Length = 594
Score = 71.0 bits (171), Expect = 2e-11
Identities = 48/165 (29%), Positives = 84/165 (50%), Gaps = 2/165 (1%)
Query: 72 RISEQLNVDVFIKDETRNPTGSFRDRLATVAIS-YGLPYAKNGFIVASDGNAAASVAAYS 130
++S++L V+ +IK E + SF+ R A +S G I AS GN A VA
Sbjct: 119 KLSDRLGVNFYIKREDKQRVFSFKLRGAYNMMSNLSREELDKGVITASAGNHAQGVALAG 178
Query: 131 ARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAIEYAKELAKLNGLYNVTPEE 190
R +V+P + K+ + A G ++ YG++ D+A +A EL++ +GL + P +
Sbjct: 179 QRLNCVAKIVMPTTTPQIKIDAVRALGGDVVLYGKTFDEAQTHALELSEKDGLKYIPPFD 238
Query: 191 NIVGLEGQKTIAFELWEEINPTH-VIVPTGSGSYLYSIYKGFKEL 234
+ ++GQ TI E+ ++ H V +P G G + + FK++
Sbjct: 239 DPGVIKGQGTIGTEINRQLKDIHAVFIPVGGGGLIAGVATFFKQI 283
>gb|AAB40319.1| (U46200) threonine synthase [Cloning vector pDG1663]
>gi|1185597|gb|AAB40326.1| (U46201) threonine synthase
[Cloning vector pDG1664]
Length = 185
Score = 70.6 bits (170), Expect = 3e-11
Identities = 48/148 (32%), Positives = 78/148 (52%), Gaps = 7/148 (4%)
Query: 211 PTHVIVPTGSGSYLYSIYKGFKELIEIGALEEMPKLIAVQTDRCNPIA-SEILGIKGECK 269
P + +P G+ + + +KGFKE E +PK+ + + I +E++ E
Sbjct: 10 PDVLAIPVGNAGNITAYWKGFKEYHEKNGTG-LPKMRGFEAEGAAAIVRNEVI----ENP 64
Query: 270 ETKALGLYVREPLMKPKVIRAIRESSGTAVLVSEEEIEKGERLLAN-EGIFAELSSAVVM 328
ET A + + P K ++A ES+G V+++EI +L+A EG+FAE S +
Sbjct: 65 ETIATAIRIGNPASWDKAVKAAEESNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASI 124
Query: 329 PALIKLVNDGFIEKGDRVVLVVTGSGLK 356
++K V G I KG +VV V+TG+GLK
Sbjct: 125 AGVLKQVKSGEIPKGSKVVAVLTGNGLK 152
>pir||D81355 threonine dehydratase (EC 4.2.1.16) Cj0828c [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6968274|emb|CAB73093.1| (AL139076) threonine
dehydratase biosynthetic [Campylobacter jejuni]
Length = 403
Score = 70.6 bits (170), Expect = 3e-11
Identities = 52/184 (28%), Positives = 91/184 (49%), Gaps = 3/184 (1%)
Query: 54 KVKKIVSLQEGGTPLIKAR-ISEQLNVDVFIKDETRNPTGSFRDRLATVAIS-YGLPYAK 111
K K+ +S TP I + +S+ ++++K+E TG+++ R A I+ +
Sbjct: 9 KAKQQISGFVNKTPFIYSSFLSDICQSEIYLKNENLQITGAYKIRGAYNKIANLSTEQKQ 68
Query: 112 NGFIVASDGNAAASVAAYSARAEKECFVVIPRKVDKGKLIQMIAFGARIIRYGESVDDAI 171
+G I AS GN A VA + + + +V+P K+ A GA +I G++ D+A
Sbjct: 69 HGVIAASAGNHAQGVAISAKKFSIKAVIVMPESTPLLKVSATKALGAEVILRGDNFDEAY 128
Query: 172 EYAKELAKLNGLYNVTPEENIVGLEGQKTIAFELWEEINPTHVIV-PTGSGSYLYSIYKG 230
+A AK N L + P E+ + GQ T+ E+ +EI+ +I+ P G G + I
Sbjct: 129 AFATSYAKENNLSFIHPFEDEFVMAGQGTLMLEMLDEISDLDMIIAPVGGGGLISGISST 188
Query: 231 FKEL 234
K++
Sbjct: 189 AKQI 192
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.138 0.396
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 166576943
Number of Sequences: 2977
Number of extensions: 7328156
Number of successful extensions: 19189
Number of sequences better than 1.0e-10: 53
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 50
Number of HSP's that attempted gapping in prelim test: 19008
Number of HSP's gapped (non-prelim): 59
length of query: 439
length of database: 189,106,746
effective HSP length: 57
effective length of query: 382
effective length of database: 154,992,987
effective search space: 59207321034
effective search space used: 59207321034
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 166 (69.1 bits)