BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1901 (asnA-1) DE:asparaginase (asnA)
(438 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75112 asparaginase (asna) PAB1901 - Pyrococcus abyssi (str... 882 0.0
pir||B71021 probable L-asparaginase - Pyrococcus horikoshii >gi|... 827 0.0
sp|O26802|ASPG_METTH PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDO... 468 e-131
sp|Q60331|ASPG_METJA PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDO... 433 e-120
pir||D72528 probable L-asparaginase APE2200 - Aeropyrum pernix (... 428 e-119
gi|11498487 asparaginase (asnA) [Archaeoglobus fulgidus] >gi|745... 414 e-115
gb|AAK41213.1| Asparinase (ansA) [Sulfolobus solfataricus] 412 e-114
gb|AAG20050.1| (AE005085) L-asparaginase; AnsA [Halobacterium sp... 307 2e-82
emb|CAC12101.1| (AL445066) L-asparaginase related protein [Therm... 281 9e-75
pir||F75193 asparaginase (EC 3.5.1.1) [similarity] - Pyrococcus ... 206 5e-52
pir||H71225 asparaginase (EC 3.5.1.1) [similarity] - Pyrococcus ... 189 6e-47
sp|P26900|ASG1_BACSU L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 184 1e-45
sp|P18840|ASG1_ECOLI L-ASPARAGINASE I (L-ASPARAGINE AMIDOHYDROLA... 157 2e-37
gb|AAA23446.1| (M26934) asparaginase (ansA) [Escherichia coli] 157 2e-37
pir||A82131 L-asparaginase I VC1995 [imported] - Vibrio cholerae... 154 2e-36
pir||T19500 hypothetical protein C27A7.5c - Caenorhabditis elega... 153 3e-36
pir||T19501 hypothetical protein C27A7.5a - Caenorhabditis elega... 153 3e-36
gb|AAF54458.1| (AE003684) CG8526 gene product [Drosophila melano... 150 2e-35
gb|AAF20016.1|AF116552_1 (AF116552) asparaginase [Dirofilaria im... 146 4e-34
gb|AAF45911.1| (AE003429) CG6428 gene product [Drosophila melano... 145 1e-33
gb|AAA62276.1| (U06943) chimeric immunoglobulin-L-asparaginase [... 140 3e-32
sp|P00805|ASG2_ECOLI L-ASPARAGINASE II PRECURSOR (L-ASPARAGINE A... 139 6e-32
pdb|3ECA|A Chain A, Asparaginase Type Ii (E.C.3.5.1.1) (Eca) >gi... 138 1e-31
dbj|BAA31197.1| (AB009372) Lysophospholipase [Rattus norvegicus] 137 3e-31
sp|P50286|ASPG_WOLSU L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 136 4e-31
pdb|4ECA|A Chain A, Asparaginase From E. Coli, Mutant T89v With ... 134 1e-30
sp|O25424|ASPG_HELPY L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 130 4e-29
dbj|BAB05343.1| (AP001512) L-asparaginase [Bacillus halodurans] 129 5e-29
sp|Q9ZLB9|ASPG_HELPJ L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 129 6e-29
pir||H81418 asparaginase (EC 3.5.1.1) cytoplasmic Cj0029 [import... 125 7e-28
prf||742507A asparaginase [Escherichia coli] 123 3e-27
pir||G83363 L-asparaginase I PA2253 [imported] - Pseudomonas aer... 123 4e-27
sp|P43843|ASG2_HAEIN PROBABLE L-ASPARAGINASE PERIPLASMIC PRECURS... 121 1e-26
sp|O68897|ASPG_PSEFL L-ASPARAGINASE PRECURSOR (L-ASPARAGINE AMID... 115 8e-25
sp|O34482|ASG2_BACSU PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDO... 111 2e-23
gi|6323184 nitrogen catabolite-regulated cell-wall L-asparaginas... 108 1e-22
pir||C83478 glutaminase-asparaginase PA1337 [imported] - Pseudom... 104 3e-21
sp|Q9X7E6|ASPG_MYCLE PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDO... 101 2e-20
pdb|4PGA|A Chain A, Glutaminase-Asparaginase From Pseudomonas 7a... 101 2e-20
sp|P10172|ASPQ_ACIGL GLUTAMINASE-ASPARAGINASE >gi|78095|pir||A28... 101 2e-20
emb|CAA31239.1| (X12746) put. L-asparagine aminohydrolase precur... 100 4e-20
gi|6320527 Asparaginase I, intracellular isozyme; Asp1p [Sacchar... 100 5e-20
sp|P06608|ASPG_ERWCH L-ASPARAGINASE PRECURSOR (L-ASPARAGINE AMID... 99 7e-20
pdb|1DJP|A Chain A, Crystal Structure Of Pseudomonas 7a Glutamin... 99 9e-20
sp|P10182|ASPQ_PSES7 GLUTAMINASE-ASPARAGINASE (PGA) 98 2e-19
sp|P30363|ASPG_BACLI L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE... 96 8e-19
pdb|3PGA|1 Chain 1, Glutaminase-Asparaginase (E.C.3.5.1.1) (Amid... 96 1e-18
gb|AAA34438.1| (J03926) asparaginase II precursor [Saccharomyces... 94 4e-18
gb|AAB31750.1| glutaminase-asparaginase, PGA=tetrameric bacteria... 92 9e-18
pir||G75283 asparaginase (EC 3.5.1.1) [similarity] - Deinococcus... 87 5e-16
pir||T43404 probable asparaginase (EC 3.5.1.1) [similarity] - fi... 85 1e-15
pir||T50284 asparaginase (EC 3.5.1.1) precursor [similarity] - f... 83 6e-15
emb|CAA05766.1| (AJ002933) L-asparaginase [Wolinella succinogenes] 76 7e-13
pir||A81823 asparaginase (EC 3.5.1.1) NMA1947 [imported] - Neiss... 72 1e-11
>pir||E75112 asparaginase (asna) PAB1901 - Pyrococcus abyssi (strain Orsay)
>gi|5458125|emb|CAB49614.1| (AJ248285) asparaginase
(asnA) [Pyrococcus abyssi]
Length = 438
Score = 882 bits (2254), Expect = 0.0
Identities = 438/438 (100%), Positives = 438/438 (100%)
Query: 1 MRVDEFLKERNINVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIA 60
MRVDEFLKERNINVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIA
Sbjct: 1 MRVDEFLKERNINVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIA 60
Query: 61 LEKIRRIEVLERAKVKPEVHFEALIEGKPGLPEVTIIGTGGTIASRIDYETGAVYPAFTA 120
LEKIRRIEVLERAKVKPEVHFEALIEGKPGLPEVTIIGTGGTIASRIDYETGAVYPAFTA
Sbjct: 61 LEKIRRIEVLERAKVKPEVHFEALIEGKPGLPEVTIIGTGGTIASRIDYETGAVYPAFTA 120
Query: 121 EELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDT 180
EELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDT
Sbjct: 121 EELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDT 180
Query: 181 MGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGE 240
MGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGE
Sbjct: 181 MGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGE 240
Query: 241 TGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVD 300
TGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVD
Sbjct: 241 TGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVD 300
Query: 301 DKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAV 360
DKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAV
Sbjct: 301 DKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAV 360
Query: 361 CMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMML 420
CMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMML
Sbjct: 361 CMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMML 420
Query: 421 TNYAGEITPYTRFDTYLR 438
TNYAGEITPYTRFDTYLR
Sbjct: 421 TNYAGEITPYTRFDTYLR 438
>pir||B71021 probable L-asparaginase - Pyrococcus horikoshii
>gi|3257887|dbj|BAA30570.1| (AP000006) 438aa long
hypothetical L-asparaginase [Pyrococcus horikoshii]
Length = 438
Score = 827 bits (2112), Expect = 0.0
Identities = 395/438 (90%), Positives = 426/438 (97%)
Query: 1 MRVDEFLKERNINVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIA 60
MRV+EFLKE+NIN+GDF+R+ KEEDGEEV YEGY+MPPYELS GDTLVLKLENGYNIGIA
Sbjct: 1 MRVEEFLKEKNINIGDFIRVIKEEDGEEVIYEGYVMPPYELSPGDTLVLKLENGYNIGIA 60
Query: 61 LEKIRRIEVLERAKVKPEVHFEALIEGKPGLPEVTIIGTGGTIASRIDYETGAVYPAFTA 120
L KIRR+EV+ERAKVKPE+HFEA IEGKP LP+VTIIGTGGTIASRIDYETGAVYPAFTA
Sbjct: 61 LSKIRRVEVIERAKVKPEIHFEAFIEGKPHLPDVTIIGTGGTIASRIDYETGAVYPAFTA 120
Query: 121 EELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDT 180
EELAKAVPEIFE+AN+KPKLLFNIFSEDMKPKHW+KIAHEVAK+LNSGD GVVVAHGTDT
Sbjct: 121 EELAKAVPEIFEIANIKPKLLFNIFSEDMKPKHWIKIAHEVAKSLNSGDSGVVVAHGTDT 180
Query: 181 MGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGE 240
MGYTAAALSFMLR+LGKPV+LVGAQRSSDRPSSDAAMNLICSVRM+TS+VAEVMVVMHGE
Sbjct: 181 MGYTAAALSFMLRDLGKPVILVGAQRSSDRPSSDAAMNLICSVRMSTSDVAEVMVVMHGE 240
Query: 241 TGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVD 300
TGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAK+WPNG+IEFLR DYR+RSD EV VD
Sbjct: 241 TGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKVWPNGKIEFLRDDYRRRSDSEVWVD 300
Query: 301 DKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAV 360
DK+EEKVALVKVYPGISSEII+F +DKGY+GIVIEGTGLGHTPNDIIPSI+RA EEG+AV
Sbjct: 301 DKLEEKVALVKVYPGISSEIIEFFIDKGYRGIVIEGTGLGHTPNDIIPSIQRATEEGIAV 360
Query: 361 CMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMML 420
CMTSQCIYGRVNLNVY+TGR+LLKAGVIPCEDMLPETAYVKLMWVLGHTQ+LEEVR+MML
Sbjct: 361 CMTSQCIYGRVNLNVYATGRRLLKAGVIPCEDMLPETAYVKLMWVLGHTQDLEEVRRMML 420
Query: 421 TNYAGEITPYTRFDTYLR 438
TNYAGEITPYTRFDTYLR
Sbjct: 421 TNYAGEITPYTRFDTYLR 438
>sp|O26802|ASPG_METTH PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|7451280|pir||C69194 L-asparaginase I -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2621792|gb|AAB85211.1| (AE000849) L-asparaginase I
[Methanobacterium thermoautotrophicum]
Length = 435
Score = 468 bits (1191), Expect = e-131
Identities = 241/431 (55%), Positives = 316/431 (72%), Gaps = 9/431 (2%)
Query: 2 RVDEFLKERNINVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIAL 61
R +FL+ +I+VGD V + K + VTYEG ++ + + +VLKLENGYNIG+ +
Sbjct: 6 RARKFLESASIDVGDMVLVEKPD----VTYEGMVLDRADDADDRHIVLKLENGYNIGVEI 61
Query: 62 EKIRRIEVLERAKVKPEVHFEALIEGK-PGLPEVTIIGTGGTIASRIDYETGAVYPAFTA 120
R IE+LE+ +P + + + P LP+V+II TGGT+AS IDY TGAV+PAFTA
Sbjct: 62 SDAR-IELLEKGS-EPRIELPPVEAAEDPELPDVSIISTGGTVASIIDYRTGAVHPAFTA 119
Query: 121 EELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDT 180
++L +A PE+ ++AN++ + +FNI SE+MKP++WV+ A V + G GVVVAHGTDT
Sbjct: 120 DDLLRANPELLDIANIRGRAVFNILSENMKPEYWVETARAVYGEIKDGADGVVVAHGTDT 179
Query: 181 MGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGE 240
M YT+AALSFMLR PVV GAQRSSDRPSSDA++N+ CSVR ATSE+AEV V MH
Sbjct: 180 MHYTSAALSFMLRT-PVPVVFTGAQRSSDRPSSDASLNIQCSVRAATSEIAEVTVCMHAT 238
Query: 241 TGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVD 300
D C HRG KVRKMHTSRRD FRS+N +P+A++ P+G I+ L ++YRKR +E+E+
Sbjct: 239 MDDLSCHLHRGVKVRKMHTSRRDTFRSMNALPLAEVTPDG-IKILEENYRKRGSDELELS 297
Query: 301 DKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAV 360
D++EE+VA +K YPGIS +II + +D+GY+GIVIEGTGLGH P+ +IP I A + GV V
Sbjct: 298 DRVEERVAFIKSYPGISPDIIKWHLDEGYRGIVIEGTGLGHCPDTLIPVIGEAHDMGVPV 357
Query: 361 CMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMML 420
MTSQC+ GRVN+NVYSTGR+LL+AGVIPC+DMLPE AYVK+ WVLG T + E R+MM
Sbjct: 358 AMTSQCLNGRVNMNVYSTGRRLLQAGVIPCDDMLPEVAYVKMCWVLGQTDDPEMAREMMR 417
Query: 421 TNYAGEITPYT 431
N AGEI T
Sbjct: 418 ENIAGEINERT 428
>sp|Q60331|ASPG_METJA PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|2129091|pir||D64302 L-asparaginase I - Methanococcus
jannaschii >gi|1592255|gb|AAB97996.1| (U67460)
asparaginase (ansA) [Methanococcus jannaschii]
Length = 417
Score = 433 bits (1102), Expect = e-120
Identities = 220/427 (51%), Positives = 309/427 (71%), Gaps = 12/427 (2%)
Query: 12 INVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIALEKIRRIEVLE 71
+NVGD +R+ ++ +EG ++P + + + +K++NGYN+GI E ++ IE++
Sbjct: 1 MNVGDVIRVETDKG----IFEGILLPS---TNENIITIKMKNGYNVGILKENVKNIEIIA 53
Query: 72 RAKVKPEVHFEAL-IEGKPGLPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEI 130
+ + KP+ L IE L ++I+ TGGT+AS++DY+TGAV+P+FTA++L +AVPE+
Sbjct: 54 KGE-KPKYELPPLNIEKNEKLKTISILSTGGTVASKVDYKTGAVHPSFTADDLIRAVPEL 112
Query: 131 FEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSF 190
++AN+K + + NI SE+MKP++W KIA E+ K + G G+V+AHGTDTM YTA+ALSF
Sbjct: 113 LDIANIKGRAVMNILSENMKPEYWRKIAEEIKKEIEEGADGIVIAHGTDTMSYTASALSF 172
Query: 191 MLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHR 250
M++ P++LVGAQRSSDRPSSDAA+NLI +V A + V VVMHGE+GDT+C H+
Sbjct: 173 MVK-ADVPIILVGAQRSSDRPSSDAALNLISAVLAAREPIKGVYVVMHGESGDTFCYLHK 231
Query: 251 GTKVRKMHTSRRDAFRSINDVPIAKIWP-NGEIEFLRKDYRKRSDEEVEVDDKIEEKVAL 309
G KVRK H+SRRDAF+SIN +P+AKI P EI +L++ + + ++VE++ +EEKVAL
Sbjct: 232 GVKVRKCHSSRRDAFKSINSIPVAKINPFTKEIIYLQEVEKSDNSKKVEINTNLEEKVAL 291
Query: 310 VKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYG 369
+KVYPG+ EII F VDK YKGIV+EGTGLGH P I I+ A ++GV V MT+Q I G
Sbjct: 292 IKVYPGMDGEIIRFYVDKEYKGIVLEGTGLGHAPEYIFEHIKYATDKGVVVVMTTQTING 351
Query: 370 RVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEITP 429
RVN+NVYS GR+L K GVI CEDM PE A VKLM++LG+ + EEV+K++ N GEI
Sbjct: 352 RVNMNVYSNGRELQKLGVIGCEDMPPEVALVKLMYLLGNYEP-EEVKKLINKNLVGEIEY 410
Query: 430 YTRFDTY 436
+RFD Y
Sbjct: 411 RSRFDAY 417
>pir||D72528 probable L-asparaginase APE2200 - Aeropyrum pernix (strain K1)
>gi|5105900|dbj|BAA81212.1| (AP000063) 427aa long
hypothetical L-asparaginase [Aeropyrum pernix]
Length = 427
Score = 428 bits (1089), Expect = e-119
Identities = 223/408 (54%), Positives = 286/408 (69%), Gaps = 5/408 (1%)
Query: 26 GEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIALEKIRRIEVLERAKVKPEVHFEALI 85
G+ + G +MP +E S DT+V+KL NGYNIG+ + + I V + L+
Sbjct: 6 GDGSVFRGVLMPKHETSHPDTVVIKLGNGYNIGVLVGEGDDIVVKGSLRPGTPGALVPLL 65
Query: 86 EG--KPGLPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFN 143
E +P V IIG GGTIASR+DYETGAV P A ELA +PE+ A+++ + LF+
Sbjct: 66 EEPLQPAEERVYIIGAGGTIASRVDYETGAVKPYLDASELATTIPELQRYASIEAEQLFS 125
Query: 144 IFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFML-RNLGKPVVLV 202
I SEDMKP W I A+ L +G GVVVAHGTDTM +TA+ALSF + L PV+L
Sbjct: 126 ILSEDMKPSMWEAIVDRAARVLEAGYDGVVVAHGTDTMAFTASALSFAFHKGLPSPVILT 185
Query: 203 GAQRSSDRPSSDAAMNLICSVRMAT-SEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSR 261
G+QRSSDRPSSDAA NL SV A+ + AEV VVMHGETGDTY LAHRG +V+KMH+SR
Sbjct: 186 GSQRSSDRPSSDAAFNLTASVLAASRAPFAEVAVVMHGETGDTYALAHRGVRVKKMHSSR 245
Query: 262 RDAFRSINDVPIAKIWP-NGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEI 320
RDAF+S+ND P+A+I+P G +E LR DYR+R + +EVD+ EE+VALVK +PG+ SE+
Sbjct: 246 RDAFQSVNDKPLARIYPFEGRVEMLRDDYRRRGESGLEVDNGFEERVALVKHFPGLISEV 305
Query: 321 IDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGR 380
ID L+D+G+KGIV+EGTG GH +D I SIERA ++GV + +T+Q ++GRVNLNVYSTGR
Sbjct: 306 IDALLDRGFKGIVVEGTGFGHVSSDAIKSIERARDQGVPIVITTQTVFGRVNLNVYSTGR 365
Query: 381 KLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEIT 428
K+L AG IP DM E AY KL W+L T+ LE VRKM N AGE++
Sbjct: 366 KMLAAGAIPAGDMTSEAAYAKLSWILARTRELEVVRKMFQRNLAGEVS 413
>gi|11498487 asparaginase (asnA) [Archaeoglobus fulgidus]
>gi|7451281|pir||B69360 asparaginase (asnA) homolog -
Archaeoglobus fulgidus >gi|2649722|gb|AAB90360.1|
(AE001043) asparaginase (asnA) [Archaeoglobus fulgidus]
Length = 418
Score = 414 bits (1054), Expect = e-115
Identities = 216/405 (53%), Positives = 293/405 (72%), Gaps = 17/405 (4%)
Query: 31 YEGYIMPPYELSAGDTLVLKLENGYNIGIALEKIRRIEVLERAKVKP-EVHFEALIEGKP 89
+EG +MP + VLKL+NGYN+G + E+LE +V+P E H L++ +
Sbjct: 29 FEGIVMPSFT----GNFVLKLDNGYNVGF-----KEYELLEVLEVEPFEPHLPELVK-RE 78
Query: 90 GLPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDM 149
GLP+V II TGGTIAS++DY TGAV FTAEE+A VPE+ E+ NV +LL+NI SE+M
Sbjct: 79 GLPDVKIISTGGTIASKVDYRTGAVTSQFTAEEIASEVPELTEICNVDAELLYNILSENM 138
Query: 150 KPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSD 209
KP++W+++A V KAL + GV++ HGTDTM ++AAALSFML + KPVV VGAQRSSD
Sbjct: 139 KPENWIELARHVYKALKDHE-GVIITHGTDTMHFSAAALSFML-STPKPVVFVGAQRSSD 196
Query: 210 RPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSIN 269
RPSSDAAMNL+C+ + AT ++ EV+V MHG T D YCL HRG KVRK HTSRRDAF+S+N
Sbjct: 197 RPSSDAAMNLLCAAKAATEDIGEVVVCMHGSTSDDYCLVHRGVKVRKNHTSRRDAFQSVN 256
Query: 270 DVPIAKI-WPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKG 328
PI +I +P+ +E+L YR R + E+++ D++E KV L+K +PG+SS+I+++ KG
Sbjct: 257 AKPIGRIDYPSLSVEWLSWRYR-RGERELKLTDRLERKVVLIKFFPGLSSDILEYYHSKG 315
Query: 329 YKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVI 388
Y+G VIEGTGLGH D I ++ R E+ V V MTSQC++GRV VY TGR +L+AGVI
Sbjct: 316 YRGFVIEGTGLGHVSTDWIDTLRRVCEDSVVV-MTSQCLWGRVCDRVYDTGRDILRAGVI 374
Query: 389 PCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEITPYTRF 433
EDMLPE A +KLMW+LG+ ++EE ++M+ + AGEI P T++
Sbjct: 375 EGEDMLPEVALIKLMWLLGN-YSIEEAKEMVKKSVAGEIEPTTQY 418
>gb|AAK41213.1| Asparinase (ansA) [Sulfolobus solfataricus]
Length = 444
Score = 412 bits (1047), Expect = e-114
Identities = 219/444 (49%), Positives = 301/444 (67%), Gaps = 24/444 (5%)
Query: 5 EFLKERNINVGDFVRITKEEDGEEVTYEGYIMPPYELSAGDTL-VLKLENGYNIGIALEK 63
+ LK NI GD + I K + + G ++P Y S D + V+KL+NGYNIGI+++
Sbjct: 11 DILKNLNIEEGDLIEIKKGD----LRIRGILLPSY--SKDDRIFVIKLDNGYNIGISIDN 64
Query: 64 IRRIEVLERAKVKP------EVHFEALIEGKPGLPEVTIIGTGGTIASRIDYETGAVYPA 117
I I+ +E+ K EVH A E+ II TGGTI SR++YETGAV PA
Sbjct: 65 ITEIKPIEKKSSKDRESERREVHNGAK-------SEIKIISTGGTIVSRVEYETGAVRPA 117
Query: 118 FTAEELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHG 177
T EE+ + +PEI E+A V ++LF+I SE+MKP+ WVKIA V KA + G+ G+V+AHG
Sbjct: 118 LTTEEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAESVKKAFDEGNTGIVIAHG 177
Query: 178 TDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMAT-SEVAEVMVV 236
TDTM YTA+AL+F LR+L PVVLVG+QRSSDRPSSD+A+NL+ +V A + EV+V
Sbjct: 178 TDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVN 237
Query: 237 MHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKI-WPNGEIEFLRKDYRKRSDE 295
MH E+ DTY L HRG KVRKMH+SRRDAF+S+ND P+AK+ W ++ L + Y + DE
Sbjct: 238 MHAESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDE 297
Query: 296 EVEVDDKIEEKVALVKVYPGISSEIID-FLVDKGYKGIVIEGTGLGHTPNDIIPSIERAV 354
+ +D K + +V L+ YPG+ + ++ L + +GI+I GTGLGHT +D I +A
Sbjct: 298 TL-LDAKFDNRVFLLYYYPGLDRDFLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKAT 356
Query: 355 EEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEE 414
++G+ + MT+QC++GRVN+NVY+TGR+LL AGV P EDMLPE A VKLMWVL H Q+LE+
Sbjct: 357 KDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVALVKLMWVLAHEQDLEK 416
Query: 415 VRKMMLTNYAGEITPYTRFDTYLR 438
++ +M+TN GEI P D + R
Sbjct: 417 IQNLMITNLVGEINPRHTLDLFPR 440
>gb|AAG20050.1| (AE005085) L-asparaginase; AnsA [Halobacterium sp. NRC-1]
Length = 427
Score = 307 bits (778), Expect = 2e-82
Identities = 183/432 (42%), Positives = 252/432 (57%), Gaps = 28/432 (6%)
Query: 15 GDFVRITKEEDGEEVTYEGYIMPPYELSAGDTLVLKLENGYNIGIALEKIRRIEVLERAK 74
GD VR+T + ++EG ++P + D LV+KL++GYN+G+ I+VL+
Sbjct: 6 GDRVRVTHGD----ASHEGVLVPS---PSDDHLVVKLDSGYNVGVDTADAD-IDVLDADA 57
Query: 75 VKP-----EVHFEALIEGKPGLPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPE 129
V E + +E LP + +I TGGTIAS +DY TGAV F AE++ +AVP+
Sbjct: 58 VTVDGDTGEDAAGSTVEFDDDLPTIALISTGGTIASTVDYRTGAVTAQFDAEDVLRAVPD 117
Query: 130 IFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALS 189
+ AN + +++ NI SE+M+P W +A V + + +G GVVV HGTDTM ++A+ALS
Sbjct: 118 LAGRANYRGRVVANILSENMEPSIWQDLAAAVREEIEAGADGVVVMHGTDTMQFSASALS 177
Query: 190 FMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAH 249
FML PVV G+QRS+DRPSSD MN +C+V A S+VAEVMV MH D C H
Sbjct: 178 FMLET-PVPVVFTGSQRSADRPSSDNVMNAVCAVEAAKSDVAEVMVCMHATESDDRCALH 236
Query: 250 RGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYR---------KRSDEEVEVD 300
RGT+VRK HTSRRDAF ++ P+ + + E D R R D ++V
Sbjct: 237 RGTRVRKTHTSRRDAFETVGATPLGYVDYDAASEAATADARGVTVEGAHAARGDATLDVA 296
Query: 301 DKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAV 360
+E V LVK PG++ ++ +G G+VIEGTGLGH +D+ +I V++G V
Sbjct: 297 SALEPAVELVKFTPGMNESLL--AACEGSAGVVIEGTGLGHVHSDLTDTIGSLVDDGTTV 354
Query: 361 CMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMML 420
MTSQC+ GRV VY TGR LL AGV+ D LP TA VKLMW L N ++ M
Sbjct: 355 VMTSQCLEGRVCDRVYDTGRDLLAAGVVEAGDTLPGTAKVKLMWALA---NADDPEAAMQ 411
Query: 421 TNYAGEITPYTR 432
+ AG +T +R
Sbjct: 412 ESVAGALTTQSR 423
>emb|CAC12101.1| (AL445066) L-asparaginase related protein [Thermoplasma
acidophilum]
Length = 409
Score = 281 bits (712), Expect = 9e-75
Identities = 162/381 (42%), Positives = 235/381 (61%), Gaps = 10/381 (2%)
Query: 47 LVLKLENGYNIGIALEKIRRIEVLERAKVKPEVHFEALIEGKPGLPEVTIIGTGGTIASR 106
+ LK NGYN+ ++ IE + + PE +E G +++++ TGGTIASR
Sbjct: 25 ITLKASNGYNMTFDRSEVEFIE--RKKEESPERKSIEAVEKGQGDRKISVLATGGTIASR 82
Query: 107 IDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALN 166
+DYETGAV P + EL +I V K + N FSE++KP W++I VA +
Sbjct: 83 VDYETGAVSPV-SDPELIFGGSDILTRFTVAVKPILNEFSENLKPADWIRIGQAVADESS 141
Query: 167 SGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMA 226
D GVVVAHGTDTM YT++AL+FM + PVV VGAQRSSDRPSSD+ N+ ++ A
Sbjct: 142 EAD-GVVVAHGTDTMAYTSSALAFMFERMRVPVVFVGAQRSSDRPSSDSRENMQAAINFA 200
Query: 227 TSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLR 286
+++ EV + MH T D + R + RKMHTSRRDAF SI P+A+ +G ++FL
Sbjct: 201 GTDLGEVGISMHASTSDGHVSLLRSVRSRKMHTSRRDAFESIGIPPLAEY--DGSVKFL- 257
Query: 287 KDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDI 346
DYR+ SD VE ++++V+++ +PG+++ ++ ++ + + +VI GTGLGH D+
Sbjct: 258 IDYRRVSD-TVEFRPDLDDRVSMIYFHPGLNAGDLENMIAEKH-AVVILGTGLGHMAKDL 315
Query: 347 IPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVL 406
IP +++ +G M SQCIYG +LNVYSTGR+LL AGVI +M+PE AYVK M++L
Sbjct: 316 IPVVKKYTADGNYAIMASQCIYGSTDLNVYSTGRELLAAGVIEAGNMVPEVAYVKAMYLL 375
Query: 407 GHTQNLEEVRKMMLTNYAGEI 427
G + + R + N GEI
Sbjct: 376 GQYPH-DLFRDLFRKNMRGEI 395
>pir||F75193 asparaginase (EC 3.5.1.1) [similarity] - Pyrococcus abyssi (strain
Orsay) >gi|5457506|emb|CAB48997.1| (AJ248283)
asparaginase related protein [Pyrococcus abyssi]
Length = 340
Score = 206 bits (518), Expect = 5e-52
Identities = 130/354 (36%), Positives = 197/354 (54%), Gaps = 41/354 (11%)
Query: 90 GLPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKL--------L 141
GL + IIG GGTIAS + E+G E A ++ EI E++ ++ K+ L
Sbjct: 10 GLMSLLIIGMGGTIAS-VKGESGY--------ESALSIEEIIEISGIREKIGSSIDTIDL 60
Query: 142 FNIFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVV 200
N+ S ++P W +A E+ + N DY G+V+ HGTDTM YTA+ LSFMLRN P+V
Sbjct: 61 MNVDSTLIQPSDWKTLAEEIER--NIWDYDGIVITHGTDTMAYTASMLSFMLRNPPVPIV 118
Query: 201 LVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTS 260
G+ + +SDA +NL +++ A + + + + +G+ L R +KVR M+
Sbjct: 119 FTGSMLPASEKNSDAPLNLYTALKFAETGIRGIYIAFNGKV----MLGVRASKVRSMNF- 173
Query: 261 RRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEI 320
DAF SIN IAK+ + +I + + Y +E D + KV ++K+ PG+S ++
Sbjct: 174 --DAFESINYPLIAKLEDDIKILHIPRIY----GDEFSSDFRYNHKVLVLKLIPGLSGDV 227
Query: 321 IDFLVDKGYKGIVIEGTGLGHTPN------DIIPSIERAVEEGVAVCMTSQCIYGRVNLN 374
+ + GY+GIVIEG G+G P ++I I R + + +T+Q +Y V+L
Sbjct: 228 FEAALKIGYRGIVIEGYGVGGIPYRGTNLFEVISKISREIP----IVLTTQALYDGVDLE 283
Query: 375 VYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEIT 428
Y GR L AG+IP DM E KLMW+LGHT+ +EEVR++M N AGE+T
Sbjct: 284 RYKVGRMALNAGIIPAGDMTKEATVTKLMWILGHTRKIEEVRELMGKNLAGELT 337
>pir||H71225 asparaginase (EC 3.5.1.1) [similarity] - Pyrococcus horikoshii
>gi|3256452|dbj|BAA29135.1| (AP000001) 328aa long
hypothetical L-asparaginase [Pyrococcus horikoshii]
Length = 328
Score = 189 bits (475), Expect = 6e-47
Identities = 122/339 (35%), Positives = 188/339 (54%), Gaps = 20/339 (5%)
Query: 94 VTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMKPKH 153
+ I+G GGTIAS + E G Y + + + I A ++ + L N+ S ++P
Sbjct: 3 ILILGMGGTIAS-VKGERG--YESALSVSKILKLAGISSEAKIEARDLMNVDSTLIQPSD 59
Query: 154 WVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSS 213
W ++A E+ K + D G+V+ HGTDTM Y+A+ LSFMLRN P+VL G+ +S
Sbjct: 60 WERLAKEIEKEVWEYD-GIVITHGTDTMAYSASMLSFMLRNPPIPIVLTGSMLPITEKNS 118
Query: 214 DAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPI 273
DA NL ++ + + + +G+ L R +K+R M DAF SIN +
Sbjct: 119 DAPFNLRTALEFVKLGIRGIYIAFNGKV----MLGVRASKIRSMGF---DAFESINYPNV 171
Query: 274 AKIWPNGEIEFLR-KDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGI 332
A+I + ++ L D+ +E D K E KV ++K+ PG+S +I+ + GYKGI
Sbjct: 172 AEI-KDDKLRILHIPDFY---GDEFFSDIKYEPKVLVIKLIPGLSGDIVREALRLGYKGI 227
Query: 333 VIEGTGLGHTP---NDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIP 389
++EG G+G P D+ + ++ + + V +T+Q IY V+L Y GR L+AGVIP
Sbjct: 228 ILEGYGVGGIPYRGTDLFEVVS-SISKRIPVVLTTQAIYDGVDLQRYKVGRIALEAGVIP 286
Query: 390 CEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEIT 428
DM E KLMW+LGHT+N+EEV+++M N GE+T
Sbjct: 287 AGDMTKEATITKLMWILGHTKNIEEVKQLMGKNITGELT 325
>sp|P26900|ASG1_BACSU L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE) >gi|98238|pir||A39440
asparaginase (EC 3.5.1.1) ansA - Bacillus subtilis
>gi|142517|gb|AAA22243.1| (M63264) L-asparaginase
[Bacillus subtilis] >gi|1303987|dbj|BAA12642.1| (D84432)
AnsA [Bacillus subtilis] >gi|2634793|emb|CAB14290.1|
(Z99116) L-asparaginase [Bacillus subtilis]
Length = 329
Score = 184 bits (463), Expect = 1e-45
Identities = 119/340 (35%), Positives = 189/340 (55%), Gaps = 16/340 (4%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMK 150
+ ++ ++ TGGTIAS ++ E G P A+EL V ++ ++ + L NI S +M+
Sbjct: 1 MKKLLMLTTGGTIAS-VEGENGLA-PGVKADELLSYVSKLDNDYTMETQSLMNIDSTNMQ 58
Query: 151 PKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDR 210
P++WV+IA V + ++ D G V+ HGTDTM YT+AALS+ML++ KP+V+ G+Q
Sbjct: 59 PEYWVEIAEAVKENYDAYD-GFVITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITF 117
Query: 211 PSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSIND 270
+DA N+ ++R A V V VV G +GT+ K+ T DAF SIN
Sbjct: 118 QKTDAKKNITDAIRFACEGVGGVYVVFDGR-------VIQGTRAIKLRTKSYDAFESINY 170
Query: 271 VPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYK 330
IA I +G IE+ K + ++ VD + V L+K++PG+ E+ D L YK
Sbjct: 171 PYIAFINEDG-IEY-NKQVTEPENDTFTVDTSLCTDVCLLKLHPGLKPEMFDALKSM-YK 227
Query: 331 GIVIEGTGLGHTP---NDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGV 387
GIVIE G G P DI+ + +E G+ V +T+QC+ ++++Y GR++ + +
Sbjct: 228 GIVIESYGSGGVPFEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLI 287
Query: 388 IPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEI 427
I +M E KLMW LG + +L V+++M T A ++
Sbjct: 288 IRSRNMNTEAIVPKLMWALGQSSDLPVVKRIMETPIADDV 327
>sp|P18840|ASG1_ECOLI L-ASPARAGINASE I (L-ASPARAGINE AMIDOHYDROLASE I) (L-ASNASE I)
>gi|7428261|pir||XDEC1 asparaginase (EC 3.5.1.1) I -
Escherichia coli >gi|1742878|dbj|BAA15558.1| (D90820)
L-asparaginase I (EC 3.5.1.1) (L-asparagine
amidohydrolase I). [Escherichia coli]
>gi|1788065|gb|AAC74837.1| (AE000271) cytoplasmic
L-asparaginase I [Escherichia coli K12]
Length = 338
Score = 157 bits (394), Expect = 2e-37
Identities = 95/287 (33%), Positives = 151/287 (52%), Gaps = 13/287 (4%)
Query: 146 SEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQ 205
S DM P+ W IA ++ + D G V+ HGTDTM YTA+ALSFML NLGKPV++ G+Q
Sbjct: 60 SSDMTPEDWQHIAEDIKAHYDDYD-GFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQ 118
Query: 206 RSSDRPSSDAAMNLICSVRMATS-EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDA 264
SD +NL+ ++ +A + + EV + + +RG + K H DA
Sbjct: 119 IPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRL-------YRGNRTTKAHADGFDA 171
Query: 265 FRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFL 324
F S N P+ + + I L + E+ V + + +V +YPGIS++++
Sbjct: 172 FASPNLPPLLEAGIH--IRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNF 229
Query: 325 VDKGYKGIVIEGTGLGHTPND--IIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKL 382
+ + K +++ G+G+ P + + ++ A + G+ V +QC+ G+VN+ Y+TG L
Sbjct: 230 LRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNAL 289
Query: 383 LKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEITP 429
AGVI DM E KL ++L + E +RK M N GE+TP
Sbjct: 290 AHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTP 336
>gb|AAA23446.1| (M26934) asparaginase (ansA) [Escherichia coli]
Length = 322
Score = 157 bits (394), Expect = 2e-37
Identities = 95/287 (33%), Positives = 151/287 (52%), Gaps = 13/287 (4%)
Query: 146 SEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQ 205
S DM P+ W IA ++ + D G V+ HGTDTM YTA+ALSFML NLGKPV++ G+Q
Sbjct: 44 SSDMTPEDWQHIAEDIKAHYDDYD-GFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQ 102
Query: 206 RSSDRPSSDAAMNLICSVRMATS-EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDA 264
SD +NL+ ++ +A + + EV + + +RG + K H DA
Sbjct: 103 IPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRL-------YRGNRTTKAHADGFDA 155
Query: 265 FRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFL 324
F S N P+ + + I L + E+ V + + +V +YPGIS++++
Sbjct: 156 FASPNLPPLLEAGIH--IRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNF 213
Query: 325 VDKGYKGIVIEGTGLGHTPND--IIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKL 382
+ + K +++ G+G+ P + + ++ A + G+ V +QC+ G+VN+ Y+TG L
Sbjct: 214 LRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNAL 273
Query: 383 LKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEITP 429
AGVI DM E KL ++L + E +RK M N GE+TP
Sbjct: 274 AHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTP 320
>pir||A82131 L-asparaginase I VC1995 [imported] - Vibrio cholerae (group O1
strain N16961) >gi|9656537|gb|AAF95143.1| (AE004274)
L-asparaginase I [Vibrio cholerae]
Length = 337
Score = 154 bits (385), Expect = 2e-36
Identities = 115/346 (33%), Positives = 174/346 (50%), Gaps = 26/346 (7%)
Query: 94 VTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKL-LFNIF------- 145
+ I TGGTI + F ++LA ++PE +P++ LF I
Sbjct: 6 IYIAYTGGTIGMKKSDHGYVPVAGFMEKQLA-SMPEFH-----RPEMPLFTIHEYDPLMD 59
Query: 146 SEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQ 205
S DM P W IA ++A + D G V+ HGTDTM YTA+ALSFM NLGKPV++ G+Q
Sbjct: 60 SSDMTPADWQLIADDIAANYDKYD-GFVILHGTDTMAYTASALSFMFENLGKPVIVTGSQ 118
Query: 206 RSSDRPSSDAAMNLICSVRMATS-EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDA 264
SD NL+ ++ +A + + EV + + RG + RK H A
Sbjct: 119 IPLADLRSDGQANLLNALHVAANYPINEVTLFFNNRL-------MRGNRSRKSHADGFSA 171
Query: 265 FRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFL 324
F S N P+ + N E+ K K S E +V+ + + ++ +YPGIS E+I
Sbjct: 172 FSSPNLPPLLEAGINIELSTNVKVDEKPSGE-FKVNPITPQPIGVITMYPGISHEVIRNT 230
Query: 325 VDKGYKGIVIEGTGLGHTPN--DIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKL 382
+ + +++ G+G+ P +++ ++ A E GV V +QC+ G+VN+ Y+TG L
Sbjct: 231 LLQPVNAMILLTFGVGNAPQNPELLAQLKAASERGVIVVNLTQCLAGKVNMGGYATGCAL 290
Query: 383 LKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGEIT 428
AGVI DM PE A KL ++L + EEV+ M GE+T
Sbjct: 291 ADAGVISGYDMTPEAALAKLHYLLSQNLSYEEVKAKMQQVLRGEMT 336
>pir||T19500 hypothetical protein C27A7.5c - Caenorhabditis elegans
>gi|3874556|emb|CAB02790.1| (Z81041) contains similarity
to Pfam domain: PF00023 (Ank repeat), Score=65.5,
E-value=3.7e-16, N=2; PF00710 (Asparaginase),
Score=174.7, E-value=5.1e-49, N=1~cDNA EST yk153h8.5
comes from this gene~cDNA EST yk430c7.3 comes from this
gene [Caenorhabdit>
Length = 715
Score = 153 bits (384), Expect = 3e-36
Identities = 124/400 (31%), Positives = 194/400 (48%), Gaps = 47/400 (11%)
Query: 64 IRRIEVLERAKVKPEVHFEALIEGKPGLPE--VTIIGTGGTIASRIDYETGAVYPAFTAE 121
+R I E E I LPE V ++ TGGTI + P + E
Sbjct: 92 LRNISATELVLEVQETAGTQQIGAPSALPESRVLVLYTGGTIGMKTTDGVYCPVPGYLPE 151
Query: 122 ELAKAVP-------------------EIFEVANVKPKLLFNIF-------SEDMKPKHWV 155
L P + V N+K ++++ I S DM W+
Sbjct: 152 VLRDIPPLNDRRYIEESYSNVAVRPYSLPPVRNMKKRVVYWIVEYEPLLDSCDMTFDDWI 211
Query: 156 KIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDA 215
+IA ++ KA + D G VV HGTDT+ YTA+ALSFM+ NLGKPV++ G+Q SD
Sbjct: 212 RIATDIKKAYHKYD-GFVVLHGTDTLAYTASALSFMMENLGKPVIITGSQIPVAEVRSDG 270
Query: 216 AMNLICSVRMATS-EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIA 274
NLI ++ A + ++ EV V + + RG + K+ S +AF S N P+A
Sbjct: 271 MENLIGALITAGNFDIPEVCVYFNNK-------LMRGNRTVKLDNSALEAFDSPNMHPLA 323
Query: 275 KIWPNGEIEFLRKDYRKRSD--EEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGI 332
++ N ++ + D RSD V + + V +++++P ++ E + + +G
Sbjct: 324 QMAINIKVNY---DSIFRSDMVAAFTVHENLCRDVGMLRIFPSMTIESVRAFLQPPTRGC 380
Query: 333 VIEGTGLGHTP---NDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIP 389
+++ G G+ P DII +++ A+ GV V SQC+ G+V++N Y+TG+ L GVIP
Sbjct: 381 ILQTFGSGNMPTRRQDIIMALKEAIARGVMVVNCSQCLKGQVDVN-YATGKILYDIGVIP 439
Query: 390 CEDMLPETAYVKLMWVLGHTQ-NLEEVRKMMLTNYAGEIT 428
DM E A KL +VLG + +L R M+ +N GE+T
Sbjct: 440 GSDMTSEAAMAKLCYVLGKDEWDLPMKRSMLQSNLRGEMT 479
>pir||T19501 hypothetical protein C27A7.5a - Caenorhabditis elegans
>gi|3874557|emb|CAB02791.1| (Z81041) predicted using
Genefinder~Similarity to E.coli L-asparaginase
(SW:P18840), contains similarity to Pfam domain: PF00023
(Ank repeat), Score=65.5, E-value=3.7e-16, N=2; PF00710
(Asparaginase), Score=174.7, E-value=5.1e-49, N=1~cDNA
EST yk9f7.3 >
Length = 713
Score = 153 bits (384), Expect = 3e-36
Identities = 124/400 (31%), Positives = 194/400 (48%), Gaps = 47/400 (11%)
Query: 64 IRRIEVLERAKVKPEVHFEALIEGKPGLPE--VTIIGTGGTIASRIDYETGAVYPAFTAE 121
+R I E E I LPE V ++ TGGTI + P + E
Sbjct: 90 LRNISATELVLEVQETAGTQQIGAPSALPESRVLVLYTGGTIGMKTTDGVYCPVPGYLPE 149
Query: 122 ELAKAVP-------------------EIFEVANVKPKLLFNIF-------SEDMKPKHWV 155
L P + V N+K ++++ I S DM W+
Sbjct: 150 VLRDIPPLNDRRYIEESYSNVAVRPYSLPPVRNMKKRVVYWIVEYEPLLDSCDMTFDDWI 209
Query: 156 KIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDA 215
+IA ++ KA + D G VV HGTDT+ YTA+ALSFM+ NLGKPV++ G+Q SD
Sbjct: 210 RIATDIKKAYHKYD-GFVVLHGTDTLAYTASALSFMMENLGKPVIITGSQIPVAEVRSDG 268
Query: 216 AMNLICSVRMATS-EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVPIA 274
NLI ++ A + ++ EV V + + RG + K+ S +AF S N P+A
Sbjct: 269 MENLIGALITAGNFDIPEVCVYFNNK-------LMRGNRTVKLDNSALEAFDSPNMHPLA 321
Query: 275 KIWPNGEIEFLRKDYRKRSD--EEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGI 332
++ N ++ + D RSD V + + V +++++P ++ E + + +G
Sbjct: 322 QMAINIKVNY---DSIFRSDMVAAFTVHENLCRDVGMLRIFPSMTIESVRAFLQPPTRGC 378
Query: 333 VIEGTGLGHTP---NDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIP 389
+++ G G+ P DII +++ A+ GV V SQC+ G+V++N Y+TG+ L GVIP
Sbjct: 379 ILQTFGSGNMPTRRQDIIMALKEAIARGVMVVNCSQCLKGQVDVN-YATGKILYDIGVIP 437
Query: 390 CEDMLPETAYVKLMWVLGHTQ-NLEEVRKMMLTNYAGEIT 428
DM E A KL +VLG + +L R M+ +N GE+T
Sbjct: 438 GSDMTSEAAMAKLCYVLGKDEWDLPMKRSMLQSNLRGEMT 477
>gb|AAF54458.1| (AE003684) CG8526 gene product [Drosophila melanogaster]
Length = 605
Score = 150 bits (376), Expect = 2e-35
Identities = 95/280 (33%), Positives = 156/280 (54%), Gaps = 15/280 (5%)
Query: 154 WVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSS 213
W +IA+EV K S D G V+ HGTDT+ Y+A+AL+FML +L KPVV GAQ S
Sbjct: 99 WKRIANEVGKTYKSYD-GFVILHGTDTLAYSASALAFMLESLNKPVVFTGAQIPIFEARS 157
Query: 214 DAAMNLICSVRMATS-EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSINDVP 272
D N + ++ +A + + EV+V + RG + K+++ A S N P
Sbjct: 158 DGRENFLGALLIAGNYSIPEVLVFFGNK-------ILRGCRSTKLNSDSFHALDSPNFAP 210
Query: 273 IAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGI 332
+ + N EI R+ +R + + + ++E+ VAL+++YPGIS+ ++ ++ + KG+
Sbjct: 211 LGRASVNIEIN-SRQIFRPCNIKPFSLHSELEKNVALLRIYPGISASVVQAILKEPTKGV 269
Query: 333 VIEGTGLGHTP---NDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIP 389
V++ G G+ P D++ + AV GV + +QC G V N+Y T + L++ GVIP
Sbjct: 270 VLQTFGAGNFPVNREDLLDELREAVHRGVIIVNITQCSAGMV-ANIYETSQGLMEVGVIP 328
Query: 390 CEDMLPETAYVKLMWVLGHTQ-NLEEVRKMMLTNYAGEIT 428
DM E A+ KL +VL + ++ +K+ML + GE+T
Sbjct: 329 GYDMTQEAAFTKLAYVLSKPEWDIPNKKKVMLLSLRGELT 368
>gb|AAF20016.1|AF116552_1 (AF116552) asparaginase [Dirofilaria immitis]
Length = 590
Score = 146 bits (365), Expect = 4e-34
Identities = 97/288 (33%), Positives = 155/288 (53%), Gaps = 15/288 (5%)
Query: 146 SEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQ 205
S DM W+ I ++ +A + G V+ HGTDT+ YTA ALSFML N+ KP+V+ GAQ
Sbjct: 86 SSDMTFDDWIHIGKDIQRAYDQY-VGFVILHGTDTLAYTACALSFMLENVRKPIVITGAQ 144
Query: 206 RSSDRPSSDAAMNLICSVRMATS-EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDA 264
SD NLI ++ +A + ++ EV V + + RG + K+ DA
Sbjct: 145 IPVCEVRSDGRENLIGALIIAANYDIPEVTVYFNNK-------LFRGNRTVKIDNRSMDA 197
Query: 265 FRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFL 324
F S N +PIA + + ++ + +R S V D++ V L++++P +S E +
Sbjct: 198 FESPNMLPIAYMDVDIKVNY-DSIFRSPSMAPFVVHDQLCRNVGLLRIFPSMSIENVRAS 256
Query: 325 VDKGYKGIVIEGTGLGHTPN---DIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRK 381
+ +G+V++ G G+ P+ DII +++AV+ G + SQC+ G+V+++ Y TG+
Sbjct: 257 LQAPIEGVVLQTFGAGNMPSHRTDIIDELKKAVDRGCIIINCSQCVRGQVDIH-YLTGKV 315
Query: 382 LLKAGVIPCEDMLPETAYVKLMWVLG-HTQNLEEVRKMMLTNYAGEIT 428
L G+IP DM E A KL +VL L E + MM+ N GE+T
Sbjct: 316 LYDMGIIPGSDMTAEAALTKLSYVLSKDCWELVEKKAMMVKNIRGELT 363
>gb|AAF45911.1| (AE003429) CG6428 gene product [Drosophila melanogaster]
Length = 631
Score = 145 bits (361), Expect = 1e-33
Identities = 96/303 (31%), Positives = 160/303 (52%), Gaps = 17/303 (5%)
Query: 130 IFEVANVKPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALS 189
I++++ P L S +M W +IA ++ ++ D G VV HGTDT+ YTA+ALS
Sbjct: 120 IYQISEYTPLL----DSSNMTMNDWARIASDIQQSYEFFD-GFVVLHGTDTLSYTASALS 174
Query: 190 FMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAH 249
FML NLGK V++ G+Q +D N ++ +A + + + + G
Sbjct: 175 FMLENLGKTVIITGSQIPIFETRTDGKDNFTSALIIAGNYIIPEVCIFFGHK------LM 228
Query: 250 RGTKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVAL 309
RG + K+ ++ DAF S N P+A+I + +++ R +R + E V ++E V L
Sbjct: 229 RGNRTVKVSSNALDAFDSPNVPPLARIGIDVNVDY-RLIFRPCTIERFCVHLNLDENVGL 287
Query: 310 VKVYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPN---DIIPSIERAVEEGVAVCMTSQC 366
++++P IS + +G+V++ G G+ P+ D+I + A E GV + +QC
Sbjct: 288 LRIFPSISHSTFRAFLAPPIRGVVLQSFGSGNIPSNRKDLIDELRAAGERGVIIINCTQC 347
Query: 367 IYGRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQ-NLEEVRKMMLTNYAG 425
G V +Y TG+ L GVIP DM PE A KL +V+G + +L+ ++MM ++ G
Sbjct: 348 PNGTV-AEIYDTGKVLCDVGVIPGFDMTPEAALAKLAYVIGKEEWSLDMKKQMMQSSLRG 406
Query: 426 EIT 428
E+T
Sbjct: 407 ELT 409
>gb|AAA62276.1| (U06943) chimeric immunoglobulin-L-asparaginase [synthetic
construct]
Length = 615
Score = 140 bits (349), Expect = 3e-32
Identities = 109/339 (32%), Positives = 165/339 (48%), Gaps = 20/339 (5%)
Query: 87 GKPGLPEVTIIGTGGTIASRIDYETGAVYPA--FTAEELAKAVPEIFEVANVKPKLLFNI 144
G LP +TI+ TGGTIA D T + Y E L AVP++ ++ANVK + + NI
Sbjct: 286 GSRSLPNITILATGGTIAGGGDSATKSNYTVGKVGVENLVNAVPQLKDIANVKGEQVVNI 345
Query: 145 FSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGA 204
S+DM W+ +A ++ + D G V+ HGTDTM TA L ++ KPVV+VGA
Sbjct: 346 GSQDMNDNVWLTLAKKINTDCDKTD-GFVITHGTDTMEETAYFLDLTVK-CDKPVVMVGA 403
Query: 205 QRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSR 261
R S S+D NL +V A + + V+VVM+ D G V K +T+
Sbjct: 404 MRPSTSMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLD-------GRDVTKTNTTD 456
Query: 262 RDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEE--KVALVKVYPGISSE 319
F+S+N P+ I NG+I++ R RK + + K+ E KV +V Y S
Sbjct: 457 VATFKSVNYGPLGYI-HNGKIDYQRTPARKHTSDTPFDVSKLNELPKVGIVYNYANASDL 515
Query: 320 IIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTG 379
LVD GY GIV G G G+ + ++ A + G AV +S+ G +
Sbjct: 516 PAKALVDAGYDGIVSAGVGNGNLYKSVFDTLATAAKTGTAVVRSSRVPTGATTQDAEVDD 575
Query: 380 RKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
K G + + P+ A V L L T++ ++++++
Sbjct: 576 AKY---GFVASGTLNPQKARVLLQLALTQTKDPQQIQQI 611
>sp|P00805|ASG2_ECOLI L-ASPARAGINASE II PRECURSOR (L-ASPARAGINE AMIDOHYDROLASE II)
(L-ASNASE II) (COLASPASE) >gi|67754|pir||XDEC
asparaginase (EC 3.5.1.1) II precursor [validated] -
Escherichia coli >gi|145277|gb|AAA23445.1| (M34234)
L-asparaginase II (ansB) (EC 3.5.1.1) [Escherichia coli]
>gi|146597|gb|AAA24062.1| (M34277) L-asparaginase II
(ansB) precursor [Escherichia coli]
>gi|882486|gb|AAA69124.1| (U28377) L-asparaginase
[Escherichia coli] >gi|1789327|gb|AAC75994.1| (AE000378)
periplasmic L-asparaginase II [Escherichia coli K12]
Length = 348
Score = 139 bits (347), Expect = 6e-32
Identities = 112/347 (32%), Positives = 169/347 (48%), Gaps = 24/347 (6%)
Query: 83 ALIEGKPG----LPEVTIIGTGGTIASRIDYETGAVYPA--FTAEELAKAVPEIFEVANV 136
AL+ G G LP +TI+ TGGTIA D T + Y E L AVP++ ++ANV
Sbjct: 11 ALVMGFSGAALALPNITILATGGTIAGGGDSATKSNYTVGKVGVENLVNAVPQLKDIANV 70
Query: 137 KPKLLFNIFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLG 196
K + + NI S+DM W+ +A ++ + D G V+ HGTDTM TA L ++
Sbjct: 71 KGEQVVNIGSQDMNDNVWLTLAKKINTDCDKTD-GFVITHGTDTMEETAYFLDLTVK-CD 128
Query: 197 KPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTK 253
KPVV+VGA R S S+D NL +V A + + V+VVM+ D G
Sbjct: 129 KPVVMVGAMRPSTSMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLD-------GRD 181
Query: 254 VRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEE--KVALVK 311
V K +T+ F+S+N P+ I NG+I++ R RK + + K+ E KV +V
Sbjct: 182 VTKTNTTDVATFKSVNYGPLGYI-HNGKIDYQRTPARKHTSDTPFDVSKLNELPKVGIVY 240
Query: 312 VYPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRV 371
Y S LVD GY GIV G G G+ + ++ A + G AV +S+ G
Sbjct: 241 NYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVFDTLATAAKTGTAVVRSSRVPTGAT 300
Query: 372 NLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
+ K G + + P+ A V L L T++ ++++++
Sbjct: 301 TQDAEVDDAKY---GFVASGTLNPQKARVLLQLALTQTKDPQQIQQI 344
>pdb|3ECA|A Chain A, Asparaginase Type Ii (E.C.3.5.1.1) (Eca)
>gi|443455|pdb|3ECA|B Chain B, Asparaginase Type Ii
(E.C.3.5.1.1) (Eca) >gi|443457|pdb|3ECA|C Chain C,
Asparaginase Type Ii (E.C.3.5.1.1) (Eca)
>gi|443459|pdb|3ECA|D Chain D, Asparaginase Type Ii
(E.C.3.5.1.1) (Eca)
Length = 326
Score = 138 bits (345), Expect = 1e-31
Identities = 108/335 (32%), Positives = 164/335 (48%), Gaps = 20/335 (5%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPA--FTAEELAKAVPEIFEVANVKPKLLFNIFSED 148
LP +TI+ TGGTIA D T + Y E L AVP++ ++ANVK + + NI S+D
Sbjct: 1 LPNITILATGGTIAGGGDSATKSNYTVGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQD 60
Query: 149 MKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSS 208
M W+ +A ++ + D G V+ HGTDTM TA L ++ KPVV+VGA R S
Sbjct: 61 MNDNVWLTLAKKINTDCDKTD-GFVITHGTDTMEETAYFLDLTVK-CDKPVVMVGAMRPS 118
Query: 209 DRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAF 265
S+D NL +V A + + V+VVM+ D G V K +T+ F
Sbjct: 119 TSMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLD-------GRDVTKTNTTDVATF 171
Query: 266 RSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEE--KVALVKVYPGISSEIIDF 323
+S+N P+ I NG+I++ R RK + + K+ E KV +V Y S
Sbjct: 172 KSVNYGPLGYI-HNGKIDYQRTPARKHTSDTPFDVSKLNELPKVGIVYNYANASDLPAKA 230
Query: 324 LVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLL 383
LVD GY GIV G G G+ + ++ A + G AV +S+ G + K
Sbjct: 231 LVDAGYDGIVSAGVGNGNLYKSVFDTLATAAKTGTAVVRSSRVPTGATTQDAEVDDAKY- 289
Query: 384 KAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
G + + P+ A V L L T++ ++++++
Sbjct: 290 --GFVASGTLNPQKARVLLQLALTQTKDPQQIQQI 322
>dbj|BAA31197.1| (AB009372) Lysophospholipase [Rattus norvegicus]
Length = 564
Score = 137 bits (341), Expect = 3e-31
Identities = 116/367 (31%), Positives = 174/367 (46%), Gaps = 42/367 (11%)
Query: 97 IGTGGTIASRIDYETGAVYPA------------FTAEELAKA--VPE---IFEVANVKPK 139
I TGGTI R E G + P EE A+A +PE + A+ +
Sbjct: 14 IYTGGTIGMRS--EGGVLVPGRGLAAVLRTLHMLHDEEYARAHSLPEDTLVLPPASSDQR 71
Query: 140 LLFNIF-------SEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFML 192
+++ + S DM WV+IA + + G VV HGTDTM + A+ LSFML
Sbjct: 72 IIYKVLECQPLFDSSDMTITEWVQIAQTIERHYTQYQ-GFVVIHGTDTMAFAASVLSFML 130
Query: 193 RNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSEV-AEVMVVMHGETGDTYCLAHRG 251
NL KPV+L GAQ SD NL+ ++ MA V EV + + RG
Sbjct: 131 ENLQKPVILTGAQVPIHELWSDGRENLLGALLMAGQYVIPEVCLFFQNQ-------LFRG 183
Query: 252 TKVRKMHTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRS-DEEVEVDDKIEEKVALV 310
+ K+ R AF S N P+A + ++ R+ RK S + V +E V L+
Sbjct: 184 NRTTKVDARRFAAFCSPNLPPLATV--GADVTINRELVRKASWKSHLVVHSNMEPDVGLL 241
Query: 311 KVYPGISSEIIDFLVDKGYKGIVIE--GTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIY 368
++YPGI + ++ + KG+V+E G+G G T D+I + A E G+ + + C+
Sbjct: 242 RLYPGIPASLVRTFLQPPLKGVVMETFGSGNGPTKPDLIQELRAAAERGLIIVNCTHCLQ 301
Query: 369 GRVNLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHT-QNLEEVRKMMLTNYAGEI 427
G V + Y+ G + AG+I DM E A KL +VLG +L + +K++ + GE+
Sbjct: 302 GAVTSD-YAPGMAMAGAGIISGFDMTSEAALAKLSYVLGQPGLSLSDRKKLLAKDLRGEM 360
Query: 428 TPYTRFD 434
T T D
Sbjct: 361 TLPTTDD 367
>sp|P50286|ASPG_WOLSU L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|1075504|pir||S52788 asparaginase (EC 3.5.1.1) -
Wolinella succinogenes >gi|7435922|pir||S74205
asparaginase (EC 3.5.1.1) - Wolinella succinogenes
>gi|2098425|pdb|1WSA|B Chain B, Structure Of
L-Asparaginase Ii Precursor >gi|2098424|pdb|1WSA|A Chain
A, Structure Of L-Asparaginase Ii Precursor
>gi|758652|emb|CAA58658.1| (X83689) L-asparaginase
[Wolinella succinogenes] >gi|895919|emb|CAA61503.1|
(X89215) asparaginase [Wolinella succinogenes]
Length = 330
Score = 136 bits (340), Expect = 4e-31
Identities = 106/342 (30%), Positives = 175/342 (50%), Gaps = 28/342 (8%)
Query: 92 PEVTIIGTGGTIAS------RIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIF 145
P+VTI+ TGGTIA + Y GAV T ++L AVP I ++A +K + + +I
Sbjct: 4 PQVTILATGGTIAGSGESSVKSSYSAGAV----TVDKLLAAVPAINDLATIKGEQISSIG 59
Query: 146 SEDMKPKHWVKIAHEVAKALNSGD-YGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGA 204
S++M K W+K+A V + L + V++ HGTDTM TA L+ +++ KPVVLVGA
Sbjct: 60 SQEMTGKVWLKLAKRVNELLAQKETEAVIITHGTDTMEETAFFLNLTVKS-QKPVVLVGA 118
Query: 205 QRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSR 261
R S+D MNL +V +A ++ + V++VM+ E H + K++T+
Sbjct: 119 MRPGSSMSADGPMNLYNAVNVAINKASTNKGVVIVMNDE-------IHAAREATKLNTTA 171
Query: 262 RDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEE--KVALVKVYPGISSE 319
+AF S N I ++ G++E+ + R + KIEE +V ++ +P +
Sbjct: 172 VNAFASPNTGKIGTVY-YGKVEYFTQSVRPHTLASEFDISKIEELPRVDILYAHPDDTDV 230
Query: 320 IIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTG 379
+++ + G KGI+ G G G+ ++E+A + GV V +S+ G
Sbjct: 231 LVNAALQAGAKGIIHAGMGNGNPFPLTQNALEKAAKSGVVVARSSRVGSGSTTQEAEVDD 290
Query: 380 RKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLT 421
+KL G + E + P+ A V LM L T + E ++K+ T
Sbjct: 291 KKL---GFVATESLNPQKARVLLMLALTKTSDREAIQKIFST 329
>pdb|4ECA|A Chain A, Asparaginase From E. Coli, Mutant T89v With Covalently
Bound Aspartate >gi|2392783|pdb|4ECA|B Chain B,
Asparaginase From E. Coli, Mutant T89v With Covalently
Bound Aspartate >gi|2392784|pdb|4ECA|C Chain C,
Asparaginase From E. Coli, Mutant T89v With Covalently
Bound Aspartate >gi|2392785|pdb|4ECA|D Chain D,
Asparaginase From E. Coli, Mutant T89v With Covalently
Bound Aspartate
Length = 326
Score = 134 bits (335), Expect = 1e-30
Identities = 106/335 (31%), Positives = 162/335 (47%), Gaps = 20/335 (5%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPA--FTAEELAKAVPEIFEVANVKPKLLFNIFSED 148
LP +TI+ TGG IA D T + Y E L AVP++ ++ANVK + + NI S+D
Sbjct: 1 LPNITILATGGXIAGGGDSATKSNYTVGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQD 60
Query: 149 MKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSS 208
M W+ +A ++ + D G V+ HG DTM TA L ++ KPVV+VGA R S
Sbjct: 61 MNDNVWLTLAKKINTDCDKTD-GFVITHGVDTMEETAYFLDLTVK-CDKPVVMVGAMRPS 118
Query: 209 DRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAF 265
S+D NL +V A + + V+VVM+ D G V K +T+ F
Sbjct: 119 TSMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLD-------GRDVTKTNTTDVATF 171
Query: 266 RSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEE--KVALVKVYPGISSEIIDF 323
+S+N P+ I NG+I++ R RK + + K+ E KV +V Y S
Sbjct: 172 KSVNYGPLGYI-HNGKIDYQRTPARKHTSDTPFDVSKLNELPKVGIVYNYANASDLPAKA 230
Query: 324 LVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLL 383
LVD GY GIV G G G+ + ++ A + G AV +S+ G + K
Sbjct: 231 LVDAGYDGIVSAGVGNGNLYKSVFDTLATAAKTGTAVVRSSRVPTGATTQDAEVDDAKY- 289
Query: 384 KAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
G + + P+ A V L L T++ ++++++
Sbjct: 290 --GFVASGTLNPQKARVLLQLALTQTKDPQQIQQI 322
>sp|O25424|ASPG_HELPY L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|7435923|pir||C64610 asparaginase (EC 3.5.1.1) II
[similarity] - Helicobacter pylori (strain 26695)
>gi|2313847|gb|AAD07772.1| (AE000585) L-asparaginase II
(ansB) [Helicobacter pylori 26695]
Length = 330
Score = 130 bits (323), Expect = 4e-29
Identities = 103/341 (30%), Positives = 180/341 (52%), Gaps = 32/341 (9%)
Query: 91 LPEVTIIGTGGTIA------SRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNI 144
LP + ++ TGGTIA S Y++G + +EL KA+P + +A ++ + + NI
Sbjct: 5 LPTIALLATGGTIAGSGASASLGSYKSGEL----GIKELLKAIPSLNRLARIQGEQISNI 60
Query: 145 FSEDMKPKHWVKIAHEVAKAL-NSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVG 203
S+DM + W K+A + L +S GVV+ HGTDT+ +A L+ +LR+ KPVVLVG
Sbjct: 61 GSQDMNEEVWFKLAKRAQELLDDSRIQGVVITHGTDTLEESAYFLNLVLRST-KPVVLVG 119
Query: 204 AQRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTS 260
A R++ S+D A+NL +V +A +E + V+VVM D + R +V K HT+
Sbjct: 120 AMRNAASLSADGALNLYNAVSVALNEKSANKGVLVVM-----DDNIFSAR--EVIKTHTT 172
Query: 261 RRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIE---EKVALVKVYPGIS 317
F+++N I ++ G+ + + RK + E +++ KV ++ + G++
Sbjct: 173 HTSTFKALNSGAIGSVY-YGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMT 231
Query: 318 SEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYS 377
++ ++ KG+VI G G G+ + +++ A + GV + +S RVN +
Sbjct: 232 PDLFQASLNSHAKGVVIAGVGNGNVSAGFLKAMQEASQMGVVIVRSS-----RVNSGEIT 286
Query: 378 TGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
+G KA I +++ P+ A V L L T N E++++M
Sbjct: 287 SGEIDDKA-FITSDNLNPQKARVLLQLALTKTNNKEKIQEM 326
>dbj|BAB05343.1| (AP001512) L-asparaginase [Bacillus halodurans]
Length = 322
Score = 129 bits (322), Expect = 5e-29
Identities = 103/335 (30%), Positives = 166/335 (48%), Gaps = 23/335 (6%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMK 150
+ +V +I TGGTIA D E G V P T L V + +A+++ NI S M
Sbjct: 1 MKKVLVIHTGGTIAMHED-EKGGVQPKET-NPLFATVESLTSIASIEVDDFLNIPSPHMT 58
Query: 151 PKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSD 209
P+ ++A + + + + GVV+ HGTDT+ TA L +L + PVV+ GA RSS+
Sbjct: 59 PELMFQLAERLKSRVGNESFDGVVITHGTDTLEETAYLLDLLL-DWEVPVVVTGAMRSSN 117
Query: 210 RPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFR 266
+D N I +V+ A ++ A+ V+VV + E H V K HTS F+
Sbjct: 118 ELGADGPHNFISAVKTAATDEAKGKGVLVVFNDEI-------HTAKNVTKTHTSNVATFQ 170
Query: 267 SINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVD 326
S PI + G + F + S +E I+ +V L+K Y G+ ++D + D
Sbjct: 171 SPQYGPIGIVTKRG-VTF----HHAPSYKESYTVSSIDHRVVLLKAYAGMDGSVVDAIAD 225
Query: 327 KGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYS---TGRKLL 383
G G+VIE G G+ P ++PSI+R + + V + S+ + G V Y+ GR L
Sbjct: 226 TGIDGLVIEAFGQGNLPPAVVPSIKRLHQANIPVVLVSRSVSGIVQ-ETYAYEGGGRHLK 284
Query: 384 KAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
GVI + + A +KL+ L T + ++++++
Sbjct: 285 DLGVIFTNGLNGQKARLKLLVALELTTDRKKLQEI 319
>sp|Q9ZLB9|ASPG_HELPJ L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|7435926|pir||D71904 asparaginase (EC 3.5.1.1) II
[similarity] - Helicobacter pylori (strain J99)
>gi|4155215|gb|AAD06238.1| (AE001498) L-asparaginase II
[Helicobacter pylori J99]
Length = 332
Score = 129 bits (321), Expect = 6e-29
Identities = 101/338 (29%), Positives = 178/338 (51%), Gaps = 24/338 (7%)
Query: 91 LPEVTIIGTGGTIA-SRIDYETGAVYPA-FTAEELAKAVPEIFEVANVKPKLLFNIFSED 148
LP + ++ TGGTIA S +D G+ +EL KA+P + ++A ++ + + NI S+D
Sbjct: 5 LPTIALLATGGTIAGSGVDASLGSYKSGELGVKELLKAIPSLNKIARIQGEQVSNIGSQD 64
Query: 149 MKPKHWVKIAHEVAKAL-NSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRS 207
M + W K+A + L +S GVV+ HGTDT+ +A L+ +L + KPVVLVGA R+
Sbjct: 65 MNEEIWFKLAQRAQELLDDSRIQGVVITHGTDTLEESAYFLNLVLHST-KPVVLVGAMRN 123
Query: 208 SDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRDA 264
+ S+D A+NL +V +A +E + V+VVM DT R +V K HT+
Sbjct: 124 ASSLSADGALNLYYAVSVAVNEKSANKGVLVVM----DDT---IFRVREVVKTHTTHIST 176
Query: 265 FRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIE---EKVALVKVYPGISSEII 321
F+++N I ++ G+ + + RK + E +++ KV ++ + G++ ++
Sbjct: 177 FKALNSGAIGSVY-YGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDLF 235
Query: 322 DFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRK 381
++ KG+VI G G G+ + +++ A + GV + +S RV ++G
Sbjct: 236 QASLNSHAKGVVIAGVGNGNVSAGFLKAMQEASQMGVVIVRSS-----RVGSGGVTSGEI 290
Query: 382 LLKA-GVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
KA G I +++ P+ A V L L T + ++++M
Sbjct: 291 DDKAYGFITSDNLNPQKARVLLQLALTKTNDKAKIQEM 328
>pir||H81418 asparaginase (EC 3.5.1.1) cytoplasmic Cj0029 [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6967534|emb|CAB72522.1| (AL139074) cytoplasmic
L-asparaginase [Campylobacter jejuni]
Length = 331
Score = 125 bits (312), Expect = 7e-28
Identities = 108/343 (31%), Positives = 177/343 (51%), Gaps = 25/343 (7%)
Query: 88 KPGLPEVTIIGTGGTIASRIDYE---TGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNI 144
K + I+GTGGTIA ID TG A + L KAVP+I ++A++ + + NI
Sbjct: 2 KKAKSRIAILGTGGTIAGFIDSTIATTGYAAGAIDIDVLIKAVPQIRDLADISWEQIANI 61
Query: 145 FSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGA 204
S +M + W+++A ++AK G GVV+ HGTDTM TA L+ +++ KPVVLVGA
Sbjct: 62 DSSNMCDEIWLRLAKKIAKLFAEGIDGVVITHGTDTMEETAYFLNLTIKS-DKPVVLVGA 120
Query: 205 QRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSR 261
R S S+D NL +V + ++ A+ VMV ++ + L+ RG V K H+
Sbjct: 121 MRPSTAISADGPKNLYNAVALVVNKEAKNKGVMVAINDK-----ILSARG--VVKTHSLN 173
Query: 262 RDAFRSINDVPIAKIWPNGEIEFLR---KDYRKRSDEEVEVDDKIEEKVALVKVYPGISS 318
DAF S + + I +G++ F K + K + +V + KV ++ Y S
Sbjct: 174 VDAFSSPDFGDLGYI-VDGKVFFYNNVIKAHTKNAPFDVSKLTSL-PKVDILYSYSNDGS 231
Query: 319 EI-IDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYS 377
+ L + G KGIV+ G+G G + ++ +++G+ V ++S+ + G V ++
Sbjct: 232 GVAAKALFEHGTKGIVVAGSGAGSIHKNQKDVLKELLKKGLKVVVSSRVVAGCVAVSDSD 291
Query: 378 TGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMML 420
K G I ED+ P+ A V LM L T + +++++ L
Sbjct: 292 E-----KLGFISAEDLNPQKARVLLMLALTKTSDPKKIQEYFL 329
>prf||742507A asparaginase [Escherichia coli]
Length = 321
Score = 123 bits (307), Expect = 3e-27
Identities = 95/274 (34%), Positives = 138/274 (49%), Gaps = 14/274 (5%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPA--FTAEELAKAVPEIFEVANVKPKLLFNIFSED 148
LP +TI+ TGGTIA D T + Y A E L AVP++ ++ANVK + + NI S+D
Sbjct: 1 LPNITILATGGTIAGGGDSATKSNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQD 60
Query: 149 MKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSS 208
M W+ +A ++ + D G V+ HGTDTM TA L ++ KP V+VGA R S
Sbjct: 61 MNDDVWLTLAKKINTDCDKTD-GFVITHGTDTMEETAYFLDLTVK-CDKP-VMVGAMRPS 117
Query: 209 DRPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSI 268
S+D NL +V A A V++ +T G V K +T+ F+S+
Sbjct: 118 TSMSADGPFNLYNAVTAADKASANRGVLVMNDT------VLDGRDVTKTNTTDVATFKSV 171
Query: 269 NDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEE--KVALVKVYPGISSEIIDFLVD 326
N P+ I +G+I++ R RK + + K+ E KV +V Y S LVD
Sbjct: 172 NYGPLGYI-HDGKIDYQRTPARKHTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVD 230
Query: 327 KGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAV 360
GY GIV G G G+ + ++ A ++G AV
Sbjct: 231 AGYDGIVSAGVGDGNLYKTVFDTLATAAKDGTAV 264
>pir||G83363 L-asparaginase I PA2253 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9948280|gb|AAG05641.1|AE004651_3
(AE004651) L-asparaginase I [Pseudomonas aeruginosa]
Length = 328
Score = 123 bits (306), Expect = 4e-27
Identities = 98/340 (28%), Positives = 164/340 (47%), Gaps = 32/340 (9%)
Query: 96 IIGTGGTIASRIDYETGAVYPAFTA------EELAKAVPEIFEVANVKPKLLFNIFSEDM 149
++ TGGTI + A F A + L A + A ++P L S +M
Sbjct: 9 VLYTGGTIGMLQTPQGLAPAGGFEARMRDHLQSLGDAPDLRWRFAELQPPL----DSANM 64
Query: 150 KPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSD 209
+W+ + + AL++G GV++ HGTDT+ Y+AAALSF+L +L PV+L G+ +
Sbjct: 65 TQGNWLAMRDAIVAALDAGHDGVLLLHGTDTLAYSAAALSFLLLDLPIPVLLTGSMLPAG 124
Query: 210 RPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSIN 269
P SDA NL+ ++R+ A + V + L H G +V K+ R DAF +
Sbjct: 125 APGSDAWDNLVGALRVLQEGHARGVQVYFND-----ALLH-GARVSKL---RSDAFDAFA 175
Query: 270 DVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGY 329
++P + + + YR+ + E +A++ +YPG+ + + L+D G
Sbjct: 176 ELPRPRHAEHAPVP-AALGYRQ---------PRREVNLAVLPLYPGLRAAHLRGLLDSGV 225
Query: 330 KGIVIEGTGLGHTPND---IIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAG 386
+ +++E G G P+D ++ + A GV + SQC YG+V VY+ G +L AG
Sbjct: 226 EALLLECYGSGTGPSDDEELLALLREAHARGVLLAAVSQCAYGQVEFGVYAAGSRLRDAG 285
Query: 387 VIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAGE 426
++ M E A KL +LG +E ++ + GE
Sbjct: 286 LLSAGGMTREAALGKLFGLLGAGLGRDEAQRWFALDLCGE 325
>sp|P43843|ASG2_HAEIN PROBABLE L-ASPARAGINASE PERIPLASMIC PRECURSOR
>gi|1074950|pir||A64090 asparaginase (EC 3.5.1.1) II
precursor - Haemophilus influenzae (strain Rd KW20)
>gi|1573752|gb|AAC22403.1| (U32758) L-asparaginase II
(ansB) [Haemophilus influenzae Rd]
Length = 349
Score = 121 bits (301), Expect = 1e-26
Identities = 99/334 (29%), Positives = 162/334 (47%), Gaps = 20/334 (5%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPA--FTAEELAKAVPEIFEVANVKPKLLFNIFSED 148
LP +TI+ TGGTIA + Y A + + L +AVPE+ +AN+K + + I S+D
Sbjct: 24 LPNITILATGGTIAGSGQSSVNSAYKAGQLSIDTLIEAVPEMKNIANIKGEQIVKIGSQD 83
Query: 149 MKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSS 208
M + W+K+A + S D G V+ HGTDTM TA L ++ KPVVLVGA R +
Sbjct: 84 MNDEVWLKLAKAINAQCKSTD-GFVITHGTDTMEETAYFLDLTVK-CEKPVVLVGAMRPA 141
Query: 209 DRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAF 265
S+D +NL +V +A + + V+V M+ E L R V K T+ F
Sbjct: 142 TEKSADGPLNLYNAVVVAADKKSSGRGVLVAMNNEV-----LGAR--DVTKTSTTAVQTF 194
Query: 266 RSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEE--KVALVKVYPGISSEIIDF 323
S N + I N ++++ R K + +K++ KV ++ Y E ++
Sbjct: 195 HSPNYGSLGYI-HNSKVDYERSPESKHTINTPFNVEKLDSLPKVGIIYAYSNAPVEPLNA 253
Query: 324 LVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLL 383
L++ GY+GIV G G G+ + +E+A ++ V V +S+ G + K
Sbjct: 254 LLNAGYQGIVSAGVGNGNVNAAHLDRLEKAAKDSVVVVRSSRVPTGYTTRDAEVDDSKY- 312
Query: 384 KAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRK 417
G + + P+ A V L L T++ + +++
Sbjct: 313 --GFVASGTLNPQKARVLLQLALTQTKDPKVIQQ 344
>sp|O68897|ASPG_PSEFL L-ASPARAGINASE PRECURSOR (L-ASPARAGINE AMIDOHYDROLASE) (L-ASNASE)
>gi|3044187|gb|AAC33155.1| (AF056495)
glutaminase-asparaginase precursor [Pseudomonas
fluorescens]
Length = 362
Score = 115 bits (286), Expect = 8e-25
Identities = 96/342 (28%), Positives = 162/342 (47%), Gaps = 19/342 (5%)
Query: 85 IEGKPGLPEVTIIGTGGTIASR-IDYETGAVYPAFTA--EELAKAVPEIFEVANVKPKLL 141
+E K L V I+ TGGTIA A Y A E+L VPE+ ++ANV+ + +
Sbjct: 28 VETKTKLANVVILATGGTIAGAGASAANSATYQAAKVGIEQLIAGVPELSQIANVRGEQV 87
Query: 142 FNIFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVV 200
I SE + ++ +++ VA+ +S D G+V+ HGTDT+ TA L+ ++ KP++
Sbjct: 88 MQIASESINNENLLQLGRRVAELADSKDVDGIVITHGTDTLEETAYFLN-LVEKTDKPII 146
Query: 201 LVGAQRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKM 257
+VG+ R S+D +NL +V +A S+ A V+V M+ E G V KM
Sbjct: 147 VVGSMRPGTAMSADGMLNLYNAVAVAGSKDARGKGVLVTMNDE-------IQSGRDVSKM 199
Query: 258 HTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDD-KIEEKVALVKVYPGI 316
+ +AF+S P+ + F R D E ++ K V + Y +
Sbjct: 200 INIKTEAFKSPWG-PLGMVVEGKSYWFRLPAKRHTMDSEFDIKTIKSLPDVEIAYGYGNV 258
Query: 317 SSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVY 376
S + L G K I+ GTG G + ++P+++ ++GV + +S G L
Sbjct: 259 SDTAVKALAQAGAKAIIHAGTGNGSVSSKVVPALQELRKQGVQIIRSSHVNAGGFVLR-- 316
Query: 377 STGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
+ + K + D+ P+ A + M L TQ+ +E+++M
Sbjct: 317 NAEQPDDKYDWVVAHDLNPQKARILAMVALTKTQDSKELQRM 358
>sp|O34482|ASG2_BACSU PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|7435924|pir||F69754 asparaginase (EC 3.5.1.1) yccC
[similarity] - Bacillus subtilis
>gi|2415720|dbj|BAA22230.1| (AB000617) YccC [Bacillus
subtilis] >gi|2632555|emb|CAB12063.1| (Z99105) similar
to asparaginase [Bacillus subtilis]
Length = 375
Score = 111 bits (275), Expect = 2e-23
Identities = 102/344 (29%), Positives = 166/344 (47%), Gaps = 36/344 (10%)
Query: 88 KPGLPEVTIIGTGGTIA-------SRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKL 140
K LP + I+ TGGTIA S +Y+ G V E L +AVPE+ ++ANV +
Sbjct: 47 KKDLPNIRILATGGTIAGADQSKTSTTEYKAGVV----GVESLIEAVPEMKDIANVSGEQ 102
Query: 141 LFNIFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPV 199
+ N+ S ++ K +K+A + L S D G+VV HGTDT+ TA L+ +++ KPV
Sbjct: 103 IVNVGSTNIDNKILLKLAKRINHLLASDDVDGIVVTHGTDTLEETAYFLNLTVKS-DKPV 161
Query: 200 VLVGAQRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRK 256
V+VG+ R S S+D NL +V++A + A+ +VV++ V K
Sbjct: 162 VIVGSMRPSTAISADGPSNLYNAVKVAGAPEAKGKGTLVVLNDRIAS-------ARYVTK 214
Query: 257 MHTSRRDAFRS----INDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKV 312
+T+ D F+S I+ N EI + + K +D V D++ + V ++
Sbjct: 215 TNTTTTDTFKSEEMGFVGTIADDIYFNNEI---TRKHTKDTDFSVSNLDELPQ-VDIIYG 270
Query: 313 YPGISSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVN 372
Y S + D V G KGIV G+G G + + AV++GV V +++ G V
Sbjct: 271 YQNDGSYLFDAAVKAGAKGIVFAGSGNGSLSDAAEKGADSAVKKGVTVVRSTRTGNGVVT 330
Query: 373 LNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVR 416
N + LL + + P+ A + LM L T + ++++
Sbjct: 331 PNQDYAEKDLLAS-----NSLNPQKARMLLMLALTKTNDPQKIQ 369
>gi|6323184 nitrogen catabolite-regulated cell-wall L-asparaginase II; Asp3-1p
[Saccharomyces cerevisiae] >gi|6323186|ref|NP_013258.1|
nitrogen catabolite-regulated cell-wall L-asparaginase
II; Asp3-2p [Saccharomyces cerevisiae]
>gi|6323187|ref|NP_013259.1| nitrogen
catabolite-regulated cell-wall L-asparaginase II;
Asp3-3p [Saccharomyces cerevisiae]
>gi|6323189|ref|NP_013261.1| nitrogen
catabolite-regulated cell-wall L-asparaginase II;
Asp3-4p [Saccharomyces cerevisiae]
>gi|2506203|sp|P11163|ASG2_YEAST L-ASPARAGINASE II
PRECURSOR (L-ASPARAGINE AMIDOHYDROLASE II) (ASP II)
>gi|2131183|pir||S68471 asparaginase (EC 3.5.1.1) II
precursor - yeast (Saccharomyces cerevisiae)
>gi|1234848|gb|AAB67479.1| (U51921) L-Asparaginase II
(Swiss Prot. accession number P11163). Note that this
ORF is longer at the 3' end than ASP3 in GenBank
accession number J03926. This gene is included in four
3.6 kb repeats present in this cosmid at the junction
with rDNA [> >gi|1234849|gb|AAB67481.1| (U51921)
L-Asparaginase II (Swiss Prot. accession number P11163).
Note that this ORF is longer at the 3' end than ASP3 in
GenBank accession number J03926. This gene is included
in four 3.6 kb repeats in this cosmid at the junction
with rDNA [Saccharo> >gi|1234850|gb|AAB67482.1| (U51921)
L-Asparaginase II (Swiss Prot. accession number P11163).
Note that this ORF is longer at the 3' end than ASP3 in
GenBank accession number J03926. This gene is included
in four 3.6 kb repeats present in this cosmid at the
junction with rDNA [> >gi|1234851|gb|AAB67484.1|
(U51921) L-Asparaginase II (Swiss Prot. accession number
P11163). Note that this ORF is longer at the 3' end
than ASP3 in GenBank accession number J03926. This gene
is included in four 3.6 kb repeats present in this
cosmid at the junction with rDNA [>
Length = 362
Score = 108 bits (267), Expect = 1e-22
Identities = 97/355 (27%), Positives = 160/355 (44%), Gaps = 34/355 (9%)
Query: 86 EGKPGLPEVTIIGTGGTIASR--IDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFN 143
E LP + I GTGGTIAS+ T T +L +AVP + E AN+ + N
Sbjct: 27 EKNSSLPSIKIFGTGGTIASKGSTSATTAGYSVGLTVNDLIEAVPSLAEKANLDYLQVSN 86
Query: 144 IFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLV 202
+ S + H + + H +++AL S DY G VV HGTDTM TA L + N KPV +
Sbjct: 87 VGSNSLNYTHLIPLYHGISEALASDDYAGAVVTHGTDTMEETAFFLDLTI-NSEKPVCIA 145
Query: 203 GAQRSSDRPSSDAAMNLICSVRMATSEVA---EVMVVMHGETGDTYCLAHRGTKVRKMHT 259
GA R + S+D MNL +V +A SE + M+ ++ G KM+
Sbjct: 146 GAMRPATATSADGPMNLYQAVSIAASEKSLGRGTMITLNDRIAS-------GFWTTKMNA 198
Query: 260 SRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIE----EKVALVKVYPG 315
+ D FR+ ++ + N ++EF + + ++ + + +V ++ Y G
Sbjct: 199 NSLDTFRA-DEQGYLGYFSNDDVEFYYPPVKPNGWQFFDISNLTDPSEIPEVIILYSYQG 257
Query: 316 ISSE-IIDFLVDKGYKGIVIEGTGLGH-TPNDIIPSIERAVEEGVAVCMTSQCIYGRV-- 371
++ E I+ + D G KGIV+ G+G G T I + + E G+ + + + G V
Sbjct: 258 LNPELIVKAVKDLGAKGIVLAGSGAGSWTATGSIVNEQLYEEYGIPIVHSRRTADGTVPP 317
Query: 372 -NLNVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAG 425
+ Y+ G L P+ + + L L ++++R + Y G
Sbjct: 318 DDAPEYAIGSGYLN----------PQKSRILLQLCLYSGYGMDQIRSVFSGVYGG 362
>pir||C83478 glutaminase-asparaginase PA1337 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9947277|gb|AAG04726.1|AE004563_3
(AE004563) glutaminase-asparaginase [Pseudomonas
aeruginosa]
Length = 362
Score = 104 bits (256), Expect = 3e-21
Identities = 94/338 (27%), Positives = 162/338 (47%), Gaps = 23/338 (6%)
Query: 91 LPEVTIIGTGGTIASR-IDYETGAVYPA--FTAEELAKAVPEIFEVANVKPKLLFNIFSE 147
L V I+ TGGTIA A Y A ++L +VP++ ++ANV+ + +F I SE
Sbjct: 34 LSNVVILATGGTIAGAGASAANSATYTAAKVPVDQLLASVPQLKDIANVRGEQVFQIASE 93
Query: 148 DMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQR 206
++ +++ VAK +S D G+V+ HGTDT+ TA L+ ++ + KP+V+VG+ R
Sbjct: 94 SFTNENLLELGKTVAKLADSDDVDGIVITHGTDTLEETAYFLT-LVEHTEKPIVVVGSMR 152
Query: 207 SSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRD 263
S+D +NL +V +A + A V++ M+ E G KM + +
Sbjct: 153 PGTAMSADGMLNLYNAVAVAGDKSARGKGVLITMNDE-------ILSGRDASKMVNIKTE 205
Query: 264 AFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKV---YPGISSEI 320
AF+S P+ + G+ + R KR E D K +A V++ Y +S
Sbjct: 206 AFKSPWG-PLGMV-VEGKSYWFRAPV-KRHTVNSEFDIKQISALAPVEIAYSYGNVSDTA 262
Query: 321 IDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGR 380
L G K I+ GTG G P ++P+++ ++GV + +S G L + +
Sbjct: 263 YKALAQAGAKAIIHAGTGNGSVPARVVPTLQELRKQGVQIIRSSHVNAGGFVLR--NAEQ 320
Query: 381 KLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
K I D+ P+ A + + TQ+ +E++++
Sbjct: 321 PDDKNDWIVAHDLNPQKARILAAVAMTKTQDSKELQRI 358
>sp|Q9X7E6|ASPG_MYCLE PROBABLE L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE)
>gi|4539132|emb|CAB39578.1| (AL049478) putative
L-asparaginase or L-glutaminase [Mycobacterium leprae]
Length = 310
Score = 101 bits (249), Expect = 2e-20
Identities = 90/327 (27%), Positives = 141/327 (42%), Gaps = 24/327 (7%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMK 150
+ +T+I TGGTI++ D++ G PA++ EL V +V + L + S +
Sbjct: 1 MSRLTVIATGGTISTSTDHD-GVRRPAYSGTELTAHVAVDVDVVD-----LMTVDSSQLI 54
Query: 151 PKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDR 210
P W +I V A +G GVVV HGTDTM TA L PVVL GA RS+D
Sbjct: 55 PTDWDRIRAAVHTATRNGARGVVVTHGTDTMEETALWLDLTYAG-NVPVVLTGAMRSADA 113
Query: 211 PSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRSIND 270
P++D NL ++ +A S A + V+ G + +RK T F
Sbjct: 114 PNADGPTNLREALAVAASPAARGVGVLVSFAGRVL----QPLGLRKAATQDLSGF----- 164
Query: 271 VPIAKIWPNGEIEFLRKDYRKRS--DEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKG 328
++ F + R + D +V +V Y G S +D V G
Sbjct: 165 --AGELLGTSSSGFALTAAKTRPYLGDLCAAD---APRVDIVAAYLGSDSVALDAYVSTG 219
Query: 329 YKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVI 388
+GIV+E G G+ ++ + R G+ + ++++ G V+ Y G L++AG +
Sbjct: 220 ARGIVLEALGSGNAGAAVVDGVRRHCHNGIVIAVSTRVPEGHVSAG-YGPGHDLVEAGAV 278
Query: 389 PCEDMLPETAYVKLMWVLGHTQNLEEV 415
+ P V LM L + +V
Sbjct: 279 LVPWLRPSQVRVLLMAALAANLPIADV 305
>pdb|4PGA|A Chain A, Glutaminase-Asparaginase From Pseudomonas 7a
>gi|2392796|pdb|4PGA|B Chain B, Glutaminase-Asparaginase
From Pseudomonas 7a
Length = 337
Score = 101 bits (249), Expect = 2e-20
Identities = 87/342 (25%), Positives = 159/342 (46%), Gaps = 19/342 (5%)
Query: 85 IEGKPGLPEVTIIGTGGTIASR-IDYETGAVYPAFTA--EELAKAVPEIFEVANVKPKLL 141
+E + L V I+ TGGTIA A Y A ++L VPE+ ++ANV+ + +
Sbjct: 3 VENQQKLANVVILATGGTIAGAGASAANSATYQAAKVGVDKLIAGVPELADLANVRGEQV 62
Query: 142 FNIFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVV 200
I SE + +K+ VA+ +S D G+V+ HGTDT+ TA L+ +++ KP+V
Sbjct: 63 MQIASESITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETAYFLN-LVQKTDKPIV 121
Query: 201 LVGAQRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKM 257
+VG+ R S+D +NL +V +A+++ + V+V M+ E G V K
Sbjct: 122 VVGSMRPGTAMSADGMLNLYNAVAVASNKDSRGKGVLVTMNDE-------IQSGRDVSKS 174
Query: 258 HTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDD-KIEEKVALVKVYPGI 316
+ +AF+S P+ + F R + E ++ +V + Y +
Sbjct: 175 INIKTEAFKSAWG-PLGMVVEGKSYWFRLPAKRHTVNSEFDIKQISSLPQVDIAYSYGNV 233
Query: 317 SSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVY 376
+ L G K ++ GTG G + ++P++++ + G + +S G L
Sbjct: 234 TDTAYKALAQNGAKALIHAGTGNGSVSSRVVPALQQLRKNGTQIIRSSHVNQGGFVLR-- 291
Query: 377 STGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
+ + K + D+ PE A + M + TQ+ +E++++
Sbjct: 292 NAEQPDDKNDWVVAHDLNPEKARILAMVAMTKTQDSKELQRI 333
>sp|P10172|ASPQ_ACIGL GLUTAMINASE-ASPARAGINASE >gi|78095|pir||A28063
glutamin-(asparagin-)ase (EC 3.5.1.38) - Acinetobacter
calcoaceticus >gi|809177|pdb|1AGX|
Glutaminase-Asparaginase (E.C.3.5.1.1)
Length = 331
Score = 101 bits (248), Expect = 2e-20
Identities = 96/337 (28%), Positives = 155/337 (45%), Gaps = 25/337 (7%)
Query: 94 VTIIGTGGTIASRIDYETG-AVYPA--FTAEELAKAVPEIFEVANVKPKLLFNIFSEDMK 150
V I+ TGGTIA T A Y A + L KAVP++ ++AN+ + SE +
Sbjct: 4 VVIVATGGTIAGAGASSTNSATYSAAKVPVDALIKAVPQVNDLANITGIQALQVASESIT 63
Query: 151 PKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSD 209
K + +A +V + GVV+ HGTDTM TA L+ ++ KP+VLVG+ R S
Sbjct: 64 DKELLSLARQVNDLVKKPSVNGVVITHGTDTMEETAFFLNLVVHT-DKPIVLVGSMRPST 122
Query: 210 RPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFR 266
S+D +NL +V +A+S A+ VMV+M+ D+ A TK +HT AF
Sbjct: 123 ALSADGPLNLYSAVALASSNEAKNKGVMVLMN----DSIFAARDVTKGINIHT---HAF- 174
Query: 267 SINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIE----EKVALVKVYPGISSEIID 322
++ G+ + R +K ++ +KI+ V +V + +
Sbjct: 175 -VSQWGALGTLVEGKPYWFRSSVKKHTNNSEFNIEKIQGDALPGVQIVYGSDNMMPDAYQ 233
Query: 323 FLVDKGYKGIVIEGTGLGHTPNDIIPSIERA-VEEGVAVCMTSQCIYGRVNLNVYSTGRK 381
G K I+ GTG G N ++P + + E+G+ + +S+ G V N K
Sbjct: 234 AFAKAGVKAIIHAGTGNGSMANYLVPEVRKLHDEQGLQIVRSSRVAQGFVLRNAEQPDDK 293
Query: 382 LLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
G I D+ P+ A + + L T + +E++ M
Sbjct: 294 Y---GWIAAHDLNPQKARLLMALALTKTNDAKEIQNM 327
>emb|CAA31239.1| (X12746) put. L-asparagine aminohydrolase precursor [Erwinia
chrysanthemi]
Length = 348
Score = 100 bits (246), Expect = 4e-20
Identities = 94/319 (29%), Positives = 145/319 (44%), Gaps = 45/319 (14%)
Query: 91 LPEVTIIGTGGTIASRI---DYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSE 147
LP + I+ TGGTIA TG A + L AVPE+ ++ANVK + N+ SE
Sbjct: 25 LPNIVILATGGTIAGSAATGTQTTGYKAGALGVDTLINAVPEVKKLANVKGEQFSNMASE 84
Query: 148 DMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQR 206
+M +K++ V + L D GVV+ HGTDT+ +A L +++ KPVV V A R
Sbjct: 85 NMTGDVVLKLSQRVNELLARDDVDGVVITHGTDTVEESAYFLHLTVKS-DKPVVFVAAMR 143
Query: 207 SSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRD 263
+ S+D MNL+ +VR+A + + VMVV++ G + K + S D
Sbjct: 144 PATAISADGPMNLLEAVRVAGDKQSRGRGVMVVINDRIGS-------ARYITKTNASTLD 196
Query: 264 AFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIE-------EKVALVKVYPGI 316
FR+ + + I N R Y+ R D+ + KV ++ Y
Sbjct: 197 TFRANEEGYLGVIIGN------RIYYQNRIDKLHTTRSVFDVRGLTSLPKVDILYGYQDD 250
Query: 317 SSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVY 376
+ D + G KGIV G G G I + +A+E+GV V +++
Sbjct: 251 PEYLYDAAIQHGVKGIVYAGMGAGSVSVRGIAGMRKALEKGVVVMRSTR----------- 299
Query: 377 STGRKLLKAGVIPCEDMLP 395
TG G++P ++ LP
Sbjct: 300 -TGN-----GIVPPDEELP 312
>gi|6320527 Asparaginase I, intracellular isozyme; Asp1p [Saccharomyces
cerevisiae] >gi|728900|sp|P38986|ASG1_YEAST
L-ASPARAGINASE I (L-ASPARAGINE AMIDOHYDROLASE I) (ASP I)
>gi|626102|pir||S48513 asparaginase (EC 3.5.1.1),
cytosolic - yeast (Saccharomyces cerevisiae)
>gi|510198|emb|CAA81794.1| (Z27406) intracellular
isozyme of L-asparaginase [Saccharomyces cerevisiae]
>gi|914995|gb|AAB64757.1| (U32517) Asp1p: Asparaginase I
[Saccharomyces cerevisiae]
Length = 381
Score = 99.8 bits (245), Expect = 5e-20
Identities = 90/345 (26%), Positives = 163/345 (47%), Gaps = 26/345 (7%)
Query: 91 LPEVTIIGTGGTIASRI--DYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSED 148
LP + I+GTGGTIAS+ +T + T ++L A+P+I +V +++ + L N+ S+D
Sbjct: 53 LPRIKILGTGGTIASKAIDSSQTAGYHVDLTIQDLLDAIPDISKVCDIEYEQLCNVDSKD 112
Query: 149 MKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSS 208
+ KI V+++L + D G+V+ HGTDT+ TA + + P+V VG+ R S
Sbjct: 113 INEDILYKIYKGVSESLQAFD-GIVITHGTDTLSETAFFIESTIDAGDVPIVFVGSMRPS 171
Query: 209 DRPSSDAAMNL---ICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAF 265
S+D MNL IC S V+V ++ + Y + K + + D+F
Sbjct: 172 TSVSADGPMNLYQAICIASNPKSRGRGVLVSLNDQISSGY-------YITKTNANSLDSF 224
Query: 266 RSINDVPIAKIWPNGEIEFLRKDYRKRSDE--EVEVDDKIEE--KVALVKVYPGISSEII 321
++ + + N EI + + + ++ VD K + +V ++ + I+
Sbjct: 225 -NVRQGYLGN-FVNNEIHYYYPPVKPQGCHKFKLRVDGKHFKLPEVCILYAHQAFPPAIV 282
Query: 322 DFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNL-NVYSTGR 380
+ + DK Y GIV+ G G P ++ E ++ + + + + + G V + NV G
Sbjct: 283 NLVADK-YDGIVLATMGAGSLPEEV---NETCMKLSLPIVYSKRSMDGMVPIANVPKKGS 338
Query: 381 KLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKMMLTNYAG 425
K + +I + PE + + L L LEE++ + Y G
Sbjct: 339 K--EDNLIASGYLSPEKSRILLQLCLAGNYTLEEIKHVFTGVYGG 381
>sp|P06608|ASPG_ERWCH L-ASPARAGINASE PRECURSOR (L-ASPARAGINE AMIDOHYDROLASE) (L-ASNASE)
>gi|78258|pir||A26054 asparaginase (EC 3.5.1.1)
precursor - Erwinia chrysanthemi
>gi|4185897|emb|CAA32884.1| (X14777) L-asparaginase
[Erwinia chrysanthemi]
Length = 348
Score = 99.5 bits (244), Expect = 7e-20
Identities = 93/319 (29%), Positives = 145/319 (45%), Gaps = 45/319 (14%)
Query: 91 LPEVTIIGTGGTIASRI---DYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSE 147
LP + I+ TGGTIA TG A + L AVPE+ ++ANVK + N+ SE
Sbjct: 25 LPNIVILATGGTIAGSAATGTQTTGYKAGALGVDTLINAVPEVKKLANVKGEQFSNMASE 84
Query: 148 DMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQR 206
+M +K++ V + L D GVV+ HGTDT+ +A L +++ KPVV V A R
Sbjct: 85 NMTGDVVLKLSQRVNELLARDDVDGVVITHGTDTVEESAYFLHLTVKS-DKPVVFVAAMR 143
Query: 207 SSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRD 263
+ S+D MNL+ +VR+A + + VMVV++ G + K + S D
Sbjct: 144 PATAISADGPMNLLEAVRVAGDKQSRGRGVMVVLNDRIGS-------ARYITKTNASTLD 196
Query: 264 AFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIE-------EKVALVKVYPGI 316
F++ + + I N R Y+ R D+ + KV ++ Y
Sbjct: 197 TFKANEEGYLGVIIGN------RIYYQNRIDKLHTTRSVFDVRGLTSLPKVDILYGYQDD 250
Query: 317 SSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVY 376
+ D + G KGIV G G G I + +A+E+GV V +++
Sbjct: 251 PEYLYDAAIQHGVKGIVYAGMGAGSVSVRGIAGMRKAMEKGVVVIRSTR----------- 299
Query: 377 STGRKLLKAGVIPCEDMLP 395
TG G++P ++ LP
Sbjct: 300 -TGN-----GIVPPDEELP 312
>pdb|1DJP|A Chain A, Crystal Structure Of Pseudomonas 7a Glutaminase-
Asparaginase With The Inhibitor Don Covalently Bound In
The Active Site >gi|6980435|pdb|1DJP|B Chain B, Crystal
Structure Of Pseudomonas 7a Glutaminase- Asparaginase
With The Inhibitor Don Covalently Bound In The Active
Site >gi|6980432|pdb|1DJO|A Chain A, Crystal Structure
Of Pseudomonas 7a Glutaminase- Asparaginase With The
Inhibitor Donv Covalently Bound In The Active Site
>gi|6980433|pdb|1DJO|B Chain B, Crystal Structure Of
Pseudomonas 7a Glutaminase- Asparaginase With The
Inhibitor Donv Covalently Bound In The Active Site
Length = 330
Score = 99.1 bits (243), Expect = 9e-20
Identities = 86/336 (25%), Positives = 156/336 (45%), Gaps = 19/336 (5%)
Query: 91 LPEVTIIGTGGTIASR-IDYETGAVYPAFTA--EELAKAVPEIFEVANVKPKLLFNIFSE 147
L V I+ TGGTIA A Y A ++L VPE+ ++ANV+ + + I SE
Sbjct: 2 LANVVILATGGTIAGAGASAANSATYQAAKVGVDKLIAGVPELADLANVRGEQVMQIASE 61
Query: 148 DMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQR 206
+ +K+ VA+ +S D G+V+ HGTDT+ TA L+ +++ KP+V+VG+ R
Sbjct: 62 SITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETAYFLN-LVQKTDKPIVVVGSMR 120
Query: 207 SSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRD 263
S+D +NL +V +A+++ + V+V M+ E G V K + +
Sbjct: 121 PGTAMSADGMLNLYNAVAVASNKDSRGKGVLVTMNDE-------IQSGRDVSKSINIKTE 173
Query: 264 AFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDD-KIEEKVALVKVYPGISSEIID 322
AF+S P+ + F R + E ++ +V + Y ++
Sbjct: 174 AFKSAWG-PLGMVVEGKSYWFRLPAKRHTVNSEFDIKQISSLPQVDIAYSYGNVTDTAYK 232
Query: 323 FLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKL 382
L G K ++ GTG G + ++P++++ + G + +S G L + +
Sbjct: 233 ALAQNGAKALIHAGTGNGSVSSRVVPALQQLRKNGTQIIRSSHVNQGGFVLR--NAEQPD 290
Query: 383 LKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
K + D+ PE A + M + TQ+ +E++++
Sbjct: 291 DKNDWVVAHDLNPEKARILAMVAMTKTQDSKELQRI 326
>sp|P10182|ASPQ_PSES7 GLUTAMINASE-ASPARAGINASE (PGA)
Length = 337
Score = 97.9 bits (240), Expect = 2e-19
Identities = 88/342 (25%), Positives = 155/342 (44%), Gaps = 19/342 (5%)
Query: 85 IEGKPGLPEVTIIGTGGTIASR-IDYETGAVYPAFTA--EELAKAVPEIFEVANVKPKLL 141
+E + L V I+ TGGTIA A Y A ++L VPE+ ++ANV+ + +
Sbjct: 3 VENQQKLANVVILATGGTIAGAGASAANSATYQAAKVGVDKLIAGVPELADLANVRGEQV 62
Query: 142 FNIFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVV 200
I SE + +K+ VA+ +S D G+V+ HGTDT+ TA L L N KP+V
Sbjct: 63 MQIASESITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETAYFLDLTL-NTDKPIV 121
Query: 201 LVGAQRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKM 257
+VG+ R S+D +NL +V +A+++ + V+V M+ E G V K
Sbjct: 122 VVGSMRPGTAMSADGMLNLYNAVAVASNKDSRGKGVLVTMNDE-------IQSGRDVSKS 174
Query: 258 HTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDD-KIEEKVALVKVYPGI 316
+ +AF+S P+ + F R + E ++ +V + Y +
Sbjct: 175 INIKTEAFKSAWG-PLGMVVEGKSYWFRLPAKRHTVNSEFDIKQISSLPQVDIAYSYGNV 233
Query: 317 SSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVY 376
+ L G K ++ GTG G + + P+++ + G + +S G L
Sbjct: 234 TDTAYKALAQNGAKALIHAGTGNGSVSSRLTPALQTLRKTGTQIIRSSHVNQGGFVLR-- 291
Query: 377 STGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
+ + K + D+ PE A + + + TQ+ +E++++
Sbjct: 292 NAEQPDDKNDWVVAHDLNPEKARILVELAMVKTQDSKELQRI 333
>sp|P30363|ASPG_BACLI L-ASPARAGINASE (L-ASPARAGINE AMIDOHYDROLASE) >gi|98122|pir||S18999
asparaginase (EC 3.5.1.1) - Bacillus licheniformis
>gi|49272|emb|CAA77574.1| (Z11497) Asparaginase
[Bacillus licheniformis]
Length = 322
Score = 96.0 bits (235), Expect = 8e-19
Identities = 93/326 (28%), Positives = 152/326 (46%), Gaps = 32/326 (9%)
Query: 93 EVTIIGTGGTIASRIDYETGAVYPAFTAEELAK--AVPEIFEVANVKPKLLFNIFSEDMK 150
+V +I TGGTIASR A + ELA+ ++PE ++ +V P F + S +
Sbjct: 4 KVALITTGGTIASRKTESGRLAAGAISGPELAEMCSLPEDVQI-DVYPA--FQLPSMHIT 60
Query: 151 PKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSD 209
+H +++ + + G Y G VV HGTDT+ TA L + + +PVV+ G+QR+ +
Sbjct: 61 FQHLLELKQTIERVFQDGGYDGAVVTHGTDTLEETAYFLDLTIED-ERPVVVTGSQRAPE 119
Query: 210 RPSSDAAMNLICSVRMATS---EVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFR 266
+ +DA N+ +V A S + A +VV + V+K+H S F
Sbjct: 120 QQGTDAYTNIRHAVYTACSPDIKGAGTVVVFNERI-------FNARYVKKVHASNLQGF- 171
Query: 267 SINDVPIAKIWPNGEIEFLRKD----YRKRSDEEVEVDDKIEEKVALVKVYPGISSEIID 322
++ G + + D Y+K +V + V +VK Y + I
Sbjct: 172 --------DVFGFGYLGIIDNDKVYVYQKLLKRDVHQLQRPLPAVDIVKCYLDGDGKFIR 223
Query: 323 FLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRK- 381
V +G +GIV+EG G G P +++ IE+A+ +GV + +T+ G V G
Sbjct: 224 AAVREGVEGIVLEGVGRGQVPPNMMADIEQALNQGVYIVITTSAEEGEVYTTYDYAGSSY 283
Query: 382 -LLKAGVIPCEDMLPETAYVKLMWVL 406
L K GVI +D + A +KL +L
Sbjct: 284 DLAKKGVILGKDYDSKKARMKLAVLL 309
>pdb|3PGA|1 Chain 1, Glutaminase-Asparaginase (E.C.3.5.1.1) (Amidohydrolase,
Asparaginase) >gi|809339|pdb|3PGA|2 Chain 2,
Glutaminase-Asparaginase (E.C.3.5.1.1) (Amidohydrolase,
Asparaginase) >gi|809340|pdb|3PGA|3 Chain 3,
Glutaminase-Asparaginase (E.C.3.5.1.1) (Amidohydrolase,
Asparaginase) >gi|809341|pdb|3PGA|4 Chain 4,
Glutaminase-Asparaginase (E.C.3.5.1.1) (Amidohydrolase,
Asparaginase)
Length = 337
Score = 95.6 bits (234), Expect = 1e-18
Identities = 87/336 (25%), Positives = 152/336 (44%), Gaps = 19/336 (5%)
Query: 91 LPEVTIIGTGGTIASR-IDYETGAVYPAFTA--EELAKAVPEIFEVANVKPKLLFNIFSE 147
L V I+ TGGTIA A Y A ++L VPE+ ++ANV+ + + I SE
Sbjct: 9 LANVVILATGGTIAGAGASAANSATYQAAKVGVDKLIAGVPELADLANVRGEQVMQIASE 68
Query: 148 DMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQR 206
+ +K+ VA+ +S D G+V+ HGTDT+ TA L L N KP+V+VG+ R
Sbjct: 69 SITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETAYFLDLTL-NTDKPIVVVGSMR 127
Query: 207 SSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRD 263
S+D +NL +V +A+++ + V+V M+ E G V K + +
Sbjct: 128 PGTAMSADGMLNLYNAVAVASNKDSRGKGVLVTMNDE-------IQSGRDVSKSINIKTE 180
Query: 264 AFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDD-KIEEKVALVKVYPGISSEIID 322
AF+S P+ + F R + E ++ +V + Y ++
Sbjct: 181 AFKSAWG-PLGMVVEGKSYWFRLPAKRHTVNSEFDIKQISSLPQVDIAYSYGNVTDTAYK 239
Query: 323 FLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKL 382
L G K ++ GTG G + + P+++ + G + +S G L + +
Sbjct: 240 ALAQNGAKALIHAGTGNGSVSSRLTPALQTLRKTGTQIIRSSHVNQGGFVLR--NAEQPD 297
Query: 383 LKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
K + D+ PE A + + + TQ+ +E++++
Sbjct: 298 DKNDWVVAHDLNPEKARILVELAMVKTQDSKELQRI 333
>gb|AAA34438.1| (J03926) asparaginase II precursor [Saccharomyces cerevisiae]
Length = 257
Score = 93.6 bits (229), Expect = 4e-18
Identities = 68/205 (33%), Positives = 100/205 (48%), Gaps = 15/205 (7%)
Query: 86 EGKPGLPEVTIIGTGGTIASR--IDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFN 143
E LP + I GTGGTIAS+ T T +L +AVP + E AN+ + N
Sbjct: 27 EKNSSLPSIKIFGTGGTIASKGSTSATTAGYSVGLTVNDLIEAVPSLAEKANLDYLQVSN 86
Query: 144 IFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVVLV 202
+ S + H + + H +++AL S DY G VV HGTDTM TA L + N KPV +
Sbjct: 87 VGSNSLNYTHLIPLYHGISEALASDDYAGAVVTHGTDTMEETAFFLDLTI-NSEKPVCIA 145
Query: 203 GAQRSSDRPSSDAAMNLICSVRMATSEVA---EVMVVMHGETGDTYCLAHRGTKVRKMHT 259
GA R + S+D MNL +V +A SE + M+ ++ G KM+
Sbjct: 146 GAMRPATATSADGPMNLYQAVSIAASEKSLGRGTMITLNDRIAS-------GFWTTKMNA 198
Query: 260 SRRDAFRSINDVPIAKIWPNGEIEF 284
+ D FR+ ++ + N ++EF
Sbjct: 199 NSLDTFRA-DEQGYLGYFSNDDVEF 222
>gb|AAB31750.1| glutaminase-asparaginase, PGA=tetrameric bacterial amidohydrolase
[Pseudomonas, 7A, Peptide, 337 aa]
Length = 337
Score = 92.4 bits (226), Expect = 9e-18
Identities = 87/342 (25%), Positives = 151/342 (43%), Gaps = 19/342 (5%)
Query: 85 IEGKPGLPEVTIIGTGGTIA-SRIDYETGAVYPAFTA--EELAKAVPEIFEVANVKPKLL 141
+E + L V I+ TGGTIA S A Y A ++L VPE+ ++ANV+ + +
Sbjct: 3 VENQQKLANVVILATGGTIAGSGASAANSATYQAAKVGVDKLIAGVPELADLANVRGEQV 62
Query: 142 FNIFSEDMKPKHWVKIAHEVAKALNSGDY-GVVVAHGTDTMGYTAAALSFMLRNLGKPVV 200
I SE + +K+ VA+ +S D G+V+ HGTDT+ TA L L N KP+V
Sbjct: 63 MQIASESITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETAYFLDLTL-NTDKPIV 121
Query: 201 LVGAQRSSDRPSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKM 257
+VG+ R S+D +NL +V +A+++ + V+V M+ E G V K
Sbjct: 122 VVGSMRPGTAMSADGMLNLYSAVAVASNKDSRGKGVLVTMNDE-------IQSGRDVSKS 174
Query: 258 HTSRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDD-KIEEKVALVKVYPGI 316
+ +AF S P+ + F R + E ++ V + Y +
Sbjct: 175 INIKTEAFLSAWG-PLGMVVEGKSYWFRLPALRHTVNSEFDIKQISSLPGVDIAYSYGNV 233
Query: 317 SSEIIDFLVDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVY 376
+ L G I+ GTG G + + P ++ + G + +S G L
Sbjct: 234 TDTAYKALAQNGALAIIHAGTGNGSVSSRLTPRLQTLRKTGTQIIRSSHVNQGGFVLR-- 291
Query: 377 STGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
+ + K + D+ P+ A + + + T + +E++++
Sbjct: 292 NAEQPDDKNDWVVAHDLNPQKARILVELAMVKTNDSKEIQRI 333
>pir||G75283 asparaginase (EC 3.5.1.1) [similarity] - Deinococcus radiodurans
(strain R1) >gi|6460166|gb|AAF11899.1|AE002066_3
(AE002066) L-asparaginase [Deinococcus radiodurans]
Length = 347
Score = 86.6 bits (211), Expect = 5e-16
Identities = 93/339 (27%), Positives = 152/339 (44%), Gaps = 38/339 (11%)
Query: 91 LPEVTIIGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMK 150
LP + +I TGGTIASR + + P + A+P + E V FN+ S +
Sbjct: 30 LPRLALIHTGGTIASRPSPDGRGLTP-----QTPPALPGL-EGVQVSEHQPFNLPSPHVT 83
Query: 151 PKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDR 210
P H ++AH + + L G VVV HGTDT+ TA L L G PVVL G+ R ++
Sbjct: 84 PAHMQQLAHLIEQ-LAGGHDAVVVTHGTDTLEETAFFLHLCL-PAGLPVVLTGSMRHAEE 141
Query: 211 PSSDAAMNLICSVRMATSEVAE---VMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRS 267
S D NL+ + ++A +VV G+ D V K+HTS DAF
Sbjct: 142 VSWDGPGNLLDAAQVALCPQTAGRGPLVVFGGDIFD-------ARTVTKVHTSAVDAFGG 194
Query: 268 INDVPIAKI---WPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFL 324
PI +I ++ + + + + V +E +V ++ Y G E
Sbjct: 195 YPG-PIGRIDRTAAGPQVHYFARPEPRPTFRPV----TLERRVEILYAYAGWQGEGYAGA 249
Query: 325 VDKGYKGIVIEGTGLGHTPNDIIPSIERAVEEGVAVCMTSQC-----IYGRVNLNVYSTG 379
+++ G+VI G G+ P +++P I A ++ V + + +YG G
Sbjct: 250 LERA-DGLVIAALGTGNLPPELLPLI-AATDKPVVLATRTHAGPILPVYGYA-----GGG 302
Query: 380 RKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRKM 418
L++AG IP + A + L+ +L + E++R++
Sbjct: 303 ATLVEAGAIPASFLNAHKARLLLLVLLNLGASREDIRRV 341
>pir||T43404 probable asparaginase (EC 3.5.1.1) [similarity] - fission yeast
(Schizosaccharomyces pombe) >gi|1906376|emb|CAA72692.1|
(Y11944) L-asparaginase [Schizosaccharomyces pombe]
>gi|7024420|emb|CAB75867.1| (AL157811) l-asparaginase
precursor [Schizosaccharomyces pombe]
Length = 360
Score = 85.4 bits (208), Expect = 1e-15
Identities = 75/260 (28%), Positives = 119/260 (44%), Gaps = 25/260 (9%)
Query: 91 LPEVTIIGTGGTIAS-------RIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFN 143
LP VTI GGTIA +DY G+V A L AVP I +N++ + N
Sbjct: 38 LPNVTIFAMGGTIAGYASSSTETVDYAAGSVGIA----TLVDAVPAIKNFSNIRGVQVTN 93
Query: 144 IFSEDMKPKHWVKIAHEVAKALNSGD-YGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLV 202
+ SE++ P + + + + + D +G+VV HGTD++ TA L M N KP+V+V
Sbjct: 94 VGSEELTPANVLNLTQLILAEVAKPDVHGIVVTHGTDSLEETAMFLD-MTVNTTKPIVVV 152
Query: 203 GAQRSSDRPSSDAAMNLICSVRMATSEVA---EVMVVMHGETGDTYCLAHRGTKVRKMHT 259
GA R S S+D MNL+ +V +A S + M++++ G + K +
Sbjct: 153 GAMRPSTAISADGPMNLLNAVVVAASNRSIGRGTMILLNDRIGSAF-------YTTKTNG 205
Query: 260 SRRDAFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKIE-EKVALVKVYPGISS 318
+ D F+S + I + F +V + E V ++ Y G++
Sbjct: 206 NMLDTFKSYEAGFLGMIL-DQRPHFFYSPATPTGKVHFDVSNTTELPAVEILYGYQGLNP 264
Query: 319 EIIDFLVDKGYKGIVIEGTG 338
+ VD G KG+V+ G G
Sbjct: 265 NLAKAAVDLGAKGLVLAGMG 284
>pir||T50284 asparaginase (EC 3.5.1.1) precursor [similarity] - fission yeast
(Schizosaccharomyces pombe) >gi|6478618|gb|AAF13924.1|
(AF192974) unknown [Schizosaccharomyces pombe]
>gi|6742162|emb|CAB69634.1| (AL137130) L-asparaginase
precursor [Schizosaccharomyces pombe]
Length = 356
Score = 83.1 bits (202), Expect = 6e-15
Identities = 95/387 (24%), Positives = 171/387 (43%), Gaps = 69/387 (17%)
Query: 58 GIALEKIRRIEVLERAKVKPEVHFEALIEGKPGLPEVTIIGTGGTIASR-------IDYE 110
GI L + ++ +L + K K + AL LP VT+ GGTIA ++Y
Sbjct: 9 GIFLVLLCQLRLLSKRKSK-STPYNAL------LPNVTVFAMGGTIAGCANSSLEIVNYI 61
Query: 111 TGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIFSEDMKPKHWVKIAHEV-AKALNSGD 169
G+V E+L +AVP I +AN+ + N+ SE++ P +K+A + A+
Sbjct: 62 PGSV----GIEKLIEAVPAIKAIANINGVQVTNMGSENLTPADVLKLAKLILAEVAKPNV 117
Query: 170 YGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGAQRSSDRPSSDAAMNLICSVRMATSE 229
+G+V+ HGTD++ TA L + + KP+V+VGA R S +D MNL+ +V +A+S
Sbjct: 118 HGIVITHGTDSLEETAMFLDLTI-STAKPIVVVGAMRPSTAIGADGPMNLLNAVAVASSN 176
Query: 230 VA---EVMVVMHGETGDTYCLAHRGTKVRKMHTSRRDAFRS---------INDVP---IA 274
+ +V+++ G + K + + D F+S +N P +
Sbjct: 177 QSMGRGTLVLLNDRIGSAF-------YTTKTNGNTLDTFKSYEAGSLGIVLNQKPFYFFS 229
Query: 275 KIWPNGEIEFLRKDYRKRSDEEVEVDDKIEEKVALVKVYPGISSEIIDFLVDKGYKGIVI 334
P G++ F + ++ +V ++ Y G++ ++ + V G KG+V+
Sbjct: 230 PAVPTGKVFFDIYNIKQL------------PRVDILYGYQGLNPKLAESAVHLGAKGLVL 277
Query: 335 EGTGL---GHTPNDIIPSIERAVEEGVAVCMTSQCIYGRVNLNVYSTGRKLLKAGVIPCE 391
G N++I S+ R E + V +Y YS+ L IP
Sbjct: 278 AAMGATSWTDDGNEVISSLIR--EHNIPV------VYSHRTAEGYSSNSCL----GIPSY 325
Query: 392 DMLPETAYVKLMWVLGHTQNLEEVRKM 418
+ P+ A LM + ++ ++ +
Sbjct: 326 FLNPQKARYMLMLAISSGYSIRDIEDL 352
>emb|CAA05766.1| (AJ002933) L-asparaginase [Wolinella succinogenes]
Length = 119
Score = 76.1 bits (184), Expect = 7e-13
Identities = 49/120 (40%), Positives = 72/120 (59%), Gaps = 12/120 (10%)
Query: 92 PEVTIIGTGGTIAS------RIDYETGAVYPAFTAEELAKAVPEIFEVANVKPKLLFNIF 145
P+VTI+ TGGTIA + Y GAV T ++L AVP I ++A +K + + +I
Sbjct: 4 PQVTILATGGTIAGSGESSVKSSYSAGAV----TVDKLLAAVPAINDLATIKGEQISSIG 59
Query: 146 SEDMKPKHWVKIAHEVAKALNSGD-YGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVGA 204
S++M K W+K+A V + L + V++ HGTDTM TA L+ +++ KPVVLVGA
Sbjct: 60 SQEMTGKVWLKLAKRVNELLAQKETEAVIITHGTDTMEETAFFLNLTVKS-QKPVVLVGA 118
>pir||A81823 asparaginase (EC 3.5.1.1) NMA1947 [imported] - Neisseria
meningitidis (group A strain Z2491)
>gi|7380579|emb|CAB85167.1| (AL162757) L-asparaginase
[Neisseria meningitidis Z2491]
Length = 329
Score = 72.2 bits (174), Expect = 1e-11
Identities = 73/284 (25%), Positives = 124/284 (42%), Gaps = 23/284 (8%)
Query: 144 IFSEDMKPKHWVKIAHEVAKALNSGDYGVVVAHGTDTMGYTAAALSFMLRNLGKPVVLVG 203
I S ++ ++W +A AL D GV+V HGTDT+ YTA + L+ L KP+VL G
Sbjct: 52 IDSSAVQLQNWCAWLDIIAAALPQYD-GVLVLHGTDTLAYTANLFALALQGLDKPIVLTG 110
Query: 204 AQRSSDRPSSDAAMNLICSVRMATSEVAEVMVVMHGETGDTYCLAHRGTKVRKMHTSRRD 263
+Q + SDA NL +V + + ++ G+ + K+ T
Sbjct: 111 SQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGK-------LYPAVGSSKVSTETAA 163
Query: 264 AFRSINDVPIAKIWPNGEIEFLRKDYRKRSDEEVEVDDKI-------EEKVALVKVYPGI 316
F + + IA+ W + + + R S + V D + + K+A+ + PG
Sbjct: 164 GFDNPHFGVIAE-WDETQGWY---NLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPGF 219
Query: 317 S-SEIIDFLVDKGYKGIVIEGTGLGHTPND--IIPSIERAVEEGVAVCMTSQCIYGRVNL 373
+ E+ D L + ++++ G G+TP D I +++ ++G + SQ GR
Sbjct: 220 AVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR-TA 278
Query: 374 NVYSTGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTQNLEEVRK 417
VY+ G +G+I ETA + + EEV K
Sbjct: 279 AVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEK 322
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.137 0.394
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 165125187
Number of Sequences: 2977
Number of extensions: 7125974
Number of successful extensions: 18436
Number of sequences better than 1.0e-10: 54
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 43
Number of HSP's that attempted gapping in prelim test: 18232
Number of HSP's gapped (non-prelim): 58
length of query: 438
length of database: 189,106,746
effective HSP length: 57
effective length of query: 381
effective length of database: 154,992,987
effective search space: 59052328047
effective search space used: 59052328047
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 166 (69.1 bits)