BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB1912 (tfIIB) DE:transcription initiation factor IIB
(300 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75110 transcription initiation factor iib PAB1912 - Pyroco... 590 e-168
sp|O59151|TF2B_PYRHO TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG... 587 e-167
sp|Q51731|TF2B_PYRFU TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG... 561 e-159
pir||S34116 transcription factor IIB - Pyrococcus woesei 559 e-158
sp|P29095|TF2B_PYRWO TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG... 491 e-138
pdb|1D3U|B Chain B, Tata-Binding ProteinTRANSCRIPTION FACTOR (II... 372 e-102
pdb|1AIS|B Chain B, Tata-Binding ProteinTRANSCRIPTION FACTOR (II... 372 e-102
emb|CAB69073.1| (AJ271467) archaeal transcription factor B [Meth... 349 2e-95
gi|11498897 transcription initiation factor IIB [Archaeoglobus f... 349 3e-95
pir||C69218 transcription initiation factor TFIIB - Methanobacte... 345 4e-94
emb|CAC12069.1| (AL445066) transcription initiation factor IIB r... 330 1e-89
gb|AAG18850.1| (AE004988) transcription initiation factor IIB; T... 312 4e-84
gb|AAG20975.1| (AE005164) transcription initiation factor IIB; T... 303 2e-81
sp|P50387|TF2B_SULSH TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG... 299 2e-80
gb|AAK40772.1| Transcription initiation factor IIB (TFIIB) homol... 298 5e-80
gb|AAF18139.1|AF205429_1 (AF205429) archaeal transcription facto... 295 4e-79
emb|CAC12074.1| (AL445066) transcription initiation factor IIB r... 294 1e-78
gb|AAG18894.1| (AE004992) transcription initiation factor IIB; T... 289 2e-77
sp|Q58192|TF2B_METJA TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG... 286 3e-76
pir||T44261 transcription initiation factor IIB [imported] - Hal... 285 5e-76
gb|AAG19212.1| (AE005017) transcription initiation factor IIB; T... 277 8e-74
gb|AAG19313.1| (AE005026) transcription initiation factor IIB; T... 268 4e-71
pir||D71137 probable transcription initiation factor IIB - Pyroc... 237 1e-61
pir||B72475 probable transcription initiation factor IIB APE2443... 193 2e-48
gb|AAK41221.1| Transcription initiation factor IIB (TFIIB) homol... 184 1e-45
gb|AAG20319.1| (AE005105) transcription initiation factor IIB; T... 182 5e-45
gb|AAG21002.1| (AE005167) transcription initiation factor IIB; T... 174 1e-42
gi|4504193 general transcription factor IIB [Homo sapiens] >gi|1... 169 4e-41
sp|P29053|TF2B_RAT TRANSCRIPTION INITIATION FACTOR IIB (TFIIB) (... 169 4e-41
gb|AAB23144.1| (S44184) S300-II, TFIIB=transcription factor [hum... 169 4e-41
sp|P29054|TF2B_XENLA TRANSCRIPTION INITIATION FACTOR IIB (TFIIB)... 168 6e-41
sp|P29052|TF2B_DROME TRANSCRIPTION INITIATION FACTOR IIB (TFIIB)... 163 3e-39
gi|11425425 general transcription factor IIB [Homo sapiens] 150 1e-35
emb|CAA84309.1| (Z34661) AtTFIIB2 [Arabidopsis thaliana] 143 3e-33
gb|AAF02810.1|AC009400_6 (AC009400) transcription initiation fac... 143 3e-33
sp|P48512|TF2B_ARATH TRANSCRIPTION INITIATION FACTOR IIB (TFIIB)... 138 8e-32
pir||T37796 probable transcription initiation factor TFIIB subun... 138 8e-32
gb|AAF71709.1| (AF220260) transcription factor TFIIB [Drosophila... 132 4e-30
sp|P48513|TF2B_SOYBN TRANSCRIPTION INITIATION FACTOR IIB (TFIIB)... 131 1e-29
sp|P42198|TF2B_HALVA TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG... 127 2e-28
sp|Q05959|TF2B_KLULA TRANSCRIPTION INITIATION FACTOR IIB (TFIIB)... 125 5e-28
pdb|1TFB| Nmr Studies Of Human General Transcription Factor Tf... 124 1e-27
pdb|1C9B|A Chain A, Crystal Structure Of A Human Tbp Core Domain... 123 2e-27
pdb|1VOL|A Chain A, Tfiib (Human Core Domain)TBP (A.THALIANA)TAT... 123 3e-27
gi|6325343 transcription factor TFIIB homolog; Sua7p [Saccharomy... 116 4e-25
gb|AAB38876.1| (U75276) TFIIB related factor hBRF [Homo sapiens] 115 5e-25
gi|4507355 TATA box binding protein (TBP)-associated factor, RNA... 113 3e-24
gb|AAF72065.1|AF262374_1 (AF262374) RNA polymerase III transcrip... 109 4e-23
gb|AAF72066.1|AF262375_1 (AF262375) RNA polymerase III transcrip... 109 4e-23
pir||T16368 hypothetical protein F45E12.2 - Caenorhabditis elega... 108 9e-23
emb|CAB89885.1| (AL354632) transcription factor iiib 70 kd subun... 105 7e-22
sp|P46070|TF3B_KLULA TRANSCRIPTION FACTOR IIIB 70 KD SUBUNIT (TF... 102 4e-21
pir||A55483 transcription initiation factor IIIB chain BRF - yea... 102 4e-21
sp|P43072|TF3B_CANAL TRANSCRIPTION FACTOR IIIB 70 KD SUBUNIT (TF... 101 1e-20
emb|CAB16810.1| (Z99708) transcription initiation factor like pr... 100 3e-20
dbj|BAA99940.1| (AP001309) transcription initiation factor IIB (... 98 1e-19
gb|AAG01118.1|AF273333_3 (AF273333) BAC19.3 [Lycopersicon escule... 97 2e-19
gi|11437381 TATA box binding protein (TBP)-associated factor, RN... 97 3e-19
pdb|1PFT| N-Terminal Domain Of Tfiib, Nmr 96 5e-19
gi|6321685 RNA polymerase III transcription factor with homology... 95 1e-18
emb|CAC12149.1| (AL445066) transcription initiation factor IIB r... 92 7e-18
pir||T01909 transcription initiation factor IIB homolog T12H20.1... 83 4e-15
gb|AAF14044.1|AC011436_28 (AC011436) putative transcription fact... 78 9e-14
pir||E72670 hypothetical protein APE0787 - Aeropyrum pernix (str... 78 9e-14
pir||T45815 hypothetical protein F28O9.220 - Arabidopsis thalian... 74 2e-12
gb|AAD32827.1|AC007659_9 (AC007659) putative transcription facto... 73 4e-12
gb|AAD14528.1| (AC006200) putative transcription factor IIIB 70 ... 69 8e-11
>pir||E75110 transcription initiation factor iib PAB1912 - Pyrococcus abyssi
(strain Orsay) >gi|5458109|emb|CAB49598.1| (AJ248285)
transcription initiation factor IIB [Pyrococcus abyssi]
Length = 300
Score = 590 bits (1505), Expect = e-168
Identities = 300/300 (100%), Positives = 300/300 (100%)
Query: 1 MTKQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGA 60
MTKQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGA
Sbjct: 1 MTKQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGA 60
Query: 61 PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT
Sbjct: 61 PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR
Sbjct: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG
Sbjct: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPVT 300
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPVT
Sbjct: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPVT 300
>sp|O59151|TF2B_PYRHO TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG (TFIIB)
>gi|7443547|pir||E71023 probable transcription
initiation factor IIB - Pyrococcus horikoshii
>gi|3257906|dbj|BAA30589.1| (AP000006) 300aa long
hypothetical transcription initiation factor IIB
[Pyrococcus horikoshii]
Length = 300
Score = 587 bits (1497), Expect = e-167
Identities = 296/300 (98%), Positives = 300/300 (99%)
Query: 1 MTKQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGA 60
MTKQ+VCPVCGSTEFIYDPERGEIVCARCGYVIEENI+DMGPEWRAFDASQREKRSRTGA
Sbjct: 1 MTKQKVCPVCGSTEFIYDPERGEIVCARCGYVIEENIIDMGPEWRAFDASQREKRSRTGA 60
Query: 61 PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT
Sbjct: 61 PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR
Sbjct: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAY+RG
Sbjct: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYRRG 240
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPVT 300
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP+T
Sbjct: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPIT 300
>sp|Q51731|TF2B_PYRFU TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG (TFIIB)
>gi|11277774|pir||T46883 transcription factor IIB
[validated] - Pyrococcus furiosus
>gi|1206029|gb|AAC43724.1| (U48391) TFIIB [Pyrococcus
furiosus]
Length = 300
Score = 561 bits (1430), Expect = e-159
Identities = 281/299 (93%), Positives = 293/299 (97%)
Query: 1 MTKQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGA 60
M KQ+VCP C S E IYDPERGEIVCA+CGYVIEENI+DMGPEWRAFDASQRE+RSRTGA
Sbjct: 1 MNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDASQRERRSRTGA 60
Query: 61 PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
PESILLHDKGLST+IGIDRSL+GLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT
Sbjct: 61 PESILLHDKGLSTEIGIDRSLSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
AQLKLP+HVEEEAARLYREAVRKGLIRGRSIESV+AACVYAACRLLKVPRTLDEI+DIAR
Sbjct: 121 AQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVYAACRLLKVPRTLDEIADIAR 180
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
V+KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEIL+EAYKRG
Sbjct: 181 VDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILDEAYKRG 240
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPV 299
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKL IKVP+
Sbjct: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLKIKVPI 299
>pir||S34116 transcription factor IIB - Pyrococcus woesei
Length = 300
Score = 559 bits (1426), Expect = e-158
Identities = 280/299 (93%), Positives = 293/299 (97%)
Query: 1 MTKQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGA 60
+ KQ+VCP C S E IYDPERGEIVCA+CGYVIEENI+DMGPEWRAFDASQRE+RSRTGA
Sbjct: 1 VNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDASQRERRSRTGA 60
Query: 61 PESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
PESILLHDKGLST+IGIDRSL+GLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT
Sbjct: 61 PESILLHDKGLSTEIGIDRSLSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
AQLKLP+HVEEEAARLYREAVRKGLIRGRSIESV+AACVYAACRLLKVPRTLDEI+DIAR
Sbjct: 121 AQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVYAACRLLKVPRTLDEIADIAR 180
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
V+KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEIL+EAYKRG
Sbjct: 181 VDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILDEAYKRG 240
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPV 299
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKL IKVP+
Sbjct: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLKIKVPI 299
>sp|P29095|TF2B_PYRWO TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG (TFIIB)
>gi|45954|emb|CAA50006.1| (X70668) transcription factor
IIB [Pyrococcus woesei]
Length = 261
Score = 491 bits (1250), Expect = e-138
Identities = 250/260 (96%), Positives = 259/260 (99%)
Query: 40 MGPEWRAFDASQREKRSRTGAPESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRL 99
MGPEWRAFDASQRE+RSRTGAPESILLHDKGLST+IGIDRSL+GLMREKMYRLRKWQSRL
Sbjct: 1 MGPEWRAFDASQRERRSRTGAPESILLHDKGLSTEIGIDRSLSGLMREKMYRLRKWQSRL 60
Query: 100 RVSDAAERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACV 159
RVSDAAERNLAFALSELDRITAQLKLP+HVEEEAARLYREAVRKGLIRGRSIESV+AACV
Sbjct: 61 RVSDAAERNLAFALSELDRITAQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACV 120
Query: 160 YAACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADEL 219
YAACRLLKVPRTLDEI+DIARV+KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADEL
Sbjct: 121 YAACRLLKVPRTLDEIADIARVDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADEL 180
Query: 220 GLSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTE 279
GLSEKVRRRAIEIL+EAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTE
Sbjct: 181 GLSEKVRRRAIEILDEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTE 240
Query: 280 VTVRNRYKELVEKLGIKVPV 299
VTVRNRYKELVEKL IKVP+
Sbjct: 241 VTVRNRYKELVEKLKIKVPI 260
>pdb|1D3U|B Chain B, Tata-Binding ProteinTRANSCRIPTION FACTOR (II)BBRE+TATA-
Box Complex From Pyrococcus Woesei
Length = 201
Score = 372 bits (944), Expect = e-102
Identities = 192/199 (96%), Positives = 198/199 (99%)
Query: 101 VSDAAERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVY 160
VSDAAERNLAFALSELDRITAQLKLP+HVEEEAARLYREAVRKGLIRGRSIESV+AACVY
Sbjct: 2 VSDAAERNLAFALSELDRITAQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVY 61
Query: 161 AACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELG 220
AACRLLKVPRTLDEI+DIARV+KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELG
Sbjct: 62 AACRLLKVPRTLDEIADIARVDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELG 121
Query: 221 LSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEV 280
LSEKVRRRAIEIL+EAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEV
Sbjct: 122 LSEKVRRRAIEILDEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEV 181
Query: 281 TVRNRYKELVEKLGIKVPV 299
TVRNRYKELVEKL IKVP+
Sbjct: 182 TVRNRYKELVEKLKIKVPI 200
>pdb|1AIS|B Chain B, Tata-Binding ProteinTRANSCRIPTION FACTOR (II)BTATA-Box
Complex From Pyrococcus Woesei
Length = 200
Score = 372 bits (944), Expect = e-102
Identities = 192/199 (96%), Positives = 198/199 (99%)
Query: 101 VSDAAERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVY 160
VSDAAERNLAFALSELDRITAQLKLP+HVEEEAARLYREAVRKGLIRGRSIESV+AACVY
Sbjct: 1 VSDAAERNLAFALSELDRITAQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVY 60
Query: 161 AACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELG 220
AACRLLKVPRTLDEI+DIARV+KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELG
Sbjct: 61 AACRLLKVPRTLDEIADIARVDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELG 120
Query: 221 LSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEV 280
LSEKVRRRAIEIL+EAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEV
Sbjct: 121 LSEKVRRRAIEILDEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEV 180
Query: 281 TVRNRYKELVEKLGIKVPV 299
TVRNRYKELVEKL IKVP+
Sbjct: 181 TVRNRYKELVEKLKIKVPI 199
>emb|CAB69073.1| (AJ271467) archaeal transcription factor B [Methanococcus
thermolithotrophicus]
Length = 339
Score = 349 bits (887), Expect = 2e-95
Identities = 182/303 (60%), Positives = 226/303 (74%), Gaps = 12/303 (3%)
Query: 3 KQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPE 62
++ +CP+CGS I D ER EIVC CG V+++N+ D+GPEWRAFD QR KRSR G P
Sbjct: 39 EELICPMCGSKNIIKDYERAEIVCETCGCVLQQNLFDVGPEWRAFDHEQRVKRSRVGPPM 98
Query: 63 SILLHDKGLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSEL 116
+ +HDKGLST I + ++ R ++YRLRKWQ R+RVSDA+ERNLAFALSEL
Sbjct: 99 TYTIHDKGLSTVIDWRNKDSYGKDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSEL 158
Query: 117 DRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEIS 176
DRI ++L LP++V E AA LYR AV KGLIRGRSIE V AA +YAACR KVPRTLDEI+
Sbjct: 159 DRIASKLGLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIA 218
Query: 177 DIARVEKKEIGRSYRFIARNLN--LTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILE 234
+ +RV++KEIGR+YRFI+R LN LTP P DYV +FA EL L +V +AI IL+
Sbjct: 219 EGSRVDRKEIGRTYRFISRELNIRLTP----TNPIDYVPRFASELKLPGEVESKAISILQ 274
Query: 235 EAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLG 294
+A ++GLTSG+ P G+ AAA+YIAS+L G +RTQREVA+VA VTEVT+RNRYKEL E L
Sbjct: 275 KANEKGLTSGRGPTGVAAAAIYIASVLHGTRRTQREVADVAGVTEVTIRNRYKELTEHLD 334
Query: 295 IKV 297
I V
Sbjct: 335 IDV 337
>gi|11498897 transcription initiation factor IIB [Archaeoglobus fulgidus]
>gi|7443549|pir||B69412 transcription initiation factor
IIB homolog - Archaeoglobus fulgidus
>gi|2649280|gb|AAB89947.1| (AE001014) transcription
initiation factor IIB [Archaeoglobus fulgidus]
Length = 326
Score = 349 bits (885), Expect = 3e-95
Identities = 177/298 (59%), Positives = 224/298 (74%), Gaps = 8/298 (2%)
Query: 6 VCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESIL 65
VCP CGS I D RGE +C CG VIE+ +D GPEWRAFD+ QR+KRSR GAP +
Sbjct: 29 VCPECGSPRLIRDYRRGEFICQDCGLVIEDTYIDAGPEWRAFDSEQRDKRSRVGAPVTYT 88
Query: 66 LHDKGLSTDIG------IDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRI 119
+HDKGLST I ++++ R +++RLRKWQ R+R+S+A ERNLAFALSELDR+
Sbjct: 89 IHDKGLSTIIDWSNKDYYGKAISVRNRAQLFRLRKWQRRIRISNATERNLAFALSELDRM 148
Query: 120 TAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIA 179
+ L LPK V E AA +YR+AV K LIRGRSIE V+AA +YAACR VPRTLDEI+ +
Sbjct: 149 ASALGLPKSVRETAAVIYRKAVEKNLIRGRSIEGVVAAALYAACRQAGVPRTLDEIATYS 208
Query: 180 RVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKR 239
RV++KEIGR+YRFI R L L K + P DY+ +F LGLS +V+++AIEI+++A +R
Sbjct: 209 RVDRKEIGRTYRFITRELGL--KLMPTSPADYIPRFCAALGLSGEVQKKAIEIIKKAEER 266
Query: 240 GLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKV 297
LTSG+ P G+ AAALY+AS+L GE+RTQREVAEVA VTEVT+RNRYKEL EKLGI++
Sbjct: 267 ELTSGRGPTGVAAAALYVASILLGERRTQREVAEVAGVTEVTIRNRYKELAEKLGIEI 324
>pir||C69218 transcription initiation factor TFIIB - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2621979|gb|AAB85383.1| (AE000864) transcription
initiation factor TFIIB [Methanobacterium
thermoautotrophicum]
Length = 310
Score = 345 bits (875), Expect = 4e-94
Identities = 177/301 (58%), Positives = 222/301 (72%), Gaps = 8/301 (2%)
Query: 3 KQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPE 62
K+ CP CGS + D ER EIVC +CG VI++N+VDMGPEWRAFD QR+KR+R GAP
Sbjct: 10 KETKCPECGSDDLRGDYERAEIVCGKCGLVIDDNLVDMGPEWRAFDHEQRDKRTRVGAPI 69
Query: 63 SILLHDKGLSTDIG------IDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSEL 116
+ +HDKGLST I R + R + YRLRKWQ ++R+S A ERNLAFALSEL
Sbjct: 70 TYTIHDKGLSTMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSEL 129
Query: 117 DRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEIS 176
DR +++L LP+ V E A+ +YR AV LIRGRSIE V+AA +YAACR VPRTLDEI+
Sbjct: 130 DRDSSRLGLPRSVREAASMVYRRAVENKLIRGRSIEGVVAASLYAACRKCNVPRTLDEIA 189
Query: 177 DIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEA 236
+++RV KKE+GR+YRF+ R LN+ K P DYV +FA ELGLS +V+ +AIEI+E A
Sbjct: 190 EVSRVSKKEVGRTYRFLTRELNI--KLPPTSPVDYVPRFASELGLSGEVQSKAIEIIEMA 247
Query: 237 YKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIK 296
+ GLTSG+ P G+ AAALYIAS+L GE +TQR+VAEVA VTEVT+RNRYKEL E+L +
Sbjct: 248 MENGLTSGRGPTGVAAAALYIASVLLGEHKTQRDVAEVAGVTEVTIRNRYKELTEQLDLG 307
Query: 297 V 297
V
Sbjct: 308 V 308
>emb|CAC12069.1| (AL445066) transcription initiation factor IIB related protein
[Thermoplasma acidophilum]
Length = 312
Score = 330 bits (837), Expect = 1e-89
Identities = 167/299 (55%), Positives = 214/299 (70%), Gaps = 12/299 (4%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP CGST I D E GE+VC CG VIE+ +D GPEWRAFD+ Q E R+R G+P + +
Sbjct: 16 CPECGSTNLIRDYEHGELVCGECGAVIEDAYIDQGPEWRAFDSEQNESRARAGSPMTYTI 75
Query: 67 HDKGLSTDIG------IDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
HDKGLSTDI RS+ R ++YRLRKWQ R++VS+AAERNL+ AL EL+R+
Sbjct: 76 HDKGLSTDISWKNKDSYGRSIPTRNRAQLYRLRKWQKRIKVSNAAERNLSQALQELERMA 135
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
+ L +P V+E AA +YR+AV++ +IRGRSIE V+A +YAACR+ VPRTL EI+ + R
Sbjct: 136 SNLSIPDDVKETAAVIYRKAVKQNMIRGRSIEGVVAGALYAACRITNVPRTLGEIASVTR 195
Query: 181 VEKKEIGRSYRFIAR--NLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYK 238
V+KKEIGR+YR ++R LN+ P K DY+++F +L LS R +A+EIL A
Sbjct: 196 VKKKEIGRTYRIMSRYLKLNIMPS----KAEDYISRFCSKLKLSMDTRNKALEILRSAEN 251
Query: 239 RGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKV 297
GLTSGK P G+ AAA+YIASL+ GE+RTQR VAEVA VTEVT+RNRYKEL EKL + V
Sbjct: 252 AGLTSGKGPTGVAAAAIYIASLMTGERRTQRAVAEVAGVTEVTIRNRYKELTEKLQLNV 310
>gb|AAG18850.1| (AE004988) transcription initiation factor IIB; TfbG [Halobacterium
sp. NRC-1]
Length = 323
Score = 312 bits (790), Expect = 4e-84
Identities = 156/297 (52%), Positives = 212/297 (70%), Gaps = 8/297 (2%)
Query: 5 RVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESI 64
R CP CGS + +R E+VC CG V+EE +D GPEWRAF+ +R+++SR GAP +
Sbjct: 27 RECPECGSDNLVKSSDRAELVCNDCGLVVEEEQIDPGPEWRAFNHQERQEKSRVGAPTTQ 86
Query: 65 LLHDKGLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDR 118
+HDKGL+T I RS++ R +M+RLRKWQ R+R DA ERNL FALSE+DR
Sbjct: 87 TMHDKGLTTTIDWKDKDAYGRSISSKKRSQMHRLRKWQERIRTKDAGERNLQFALSEIDR 146
Query: 119 ITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDI 178
+ + L +P+ V E A+ +YR A+++ LIRGRSIE V + +YAACR +PR+L+EIS++
Sbjct: 147 MASALGVPRSVREVASVIYRRALKEDLIRGRSIEGVATSALYAACRKEGIPRSLEEISEV 206
Query: 179 ARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYK 238
+RVE+KEIGR+YR+I++ L L K V P YV +F EL L+E+V+ +A EI+E +
Sbjct: 207 SRVERKEIGRTYRYISQELGLEMKP--VDPKKYVPRFCSELELTEEVQSKANEIIETTAE 264
Query: 239 RGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGI 295
GL SGKSP G AAA+Y ASLL EK+TQREVA+VA+VTEVT+RNRY+E +E +GI
Sbjct: 265 EGLLSGKSPTGYAAAAIYAASLLCNEKKTQREVADVAQVTEVTIRNRYQEQIEAMGI 321
>gb|AAG20975.1| (AE005164) transcription initiation factor IIB; TfbC [Halobacterium
sp. NRC-1]
Length = 317
Score = 303 bits (768), Expect = 2e-81
Identities = 155/300 (51%), Positives = 215/300 (71%), Gaps = 16/300 (5%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP C S I D + E VC CG V+E++ +D GPEWRAF++S+R+++SR GAP + +
Sbjct: 25 CPECNSANVITD--QSERVCEDCGLVLEDDQIDHGPEWRAFNSSERDQKSRVGAPTTKTM 82
Query: 67 HDKGLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
HDKGL+T I RSL R +M RLRKWQ R+R DA ERNL FALSE+DR+
Sbjct: 83 HDKGLTTQIDWKDKDAYGRSLDAKKRNQMNRLRKWQERIRTKDAGERNLQFALSEIDRMA 142
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
+ L +P+ V E A+ +YR A+++ LIRGRSIE V AC+YAACR +PRTL+E++++AR
Sbjct: 143 SALGVPRSVREVASVIYRRALKEDLIRGRSIEGVATACLYAACRQEGIPRTLEEVTEVAR 202
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTD---YVNKFADELGLSEKVRRRAIEILEEAY 237
+++KEIGR+YR++A+ L+L ++PTD Y+ +FA +L LSE+V +A EI++ +
Sbjct: 203 IDQKEIGRTYRYVAQELSLE-----IQPTDPKEYLPRFASDLELSEEVIAKAREIIDTSA 257
Query: 238 KRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKV 297
++GL SGKSP+G AAA+Y ASLL EK+TQREVA VA VTEVT+RNRY+E +E +G V
Sbjct: 258 EQGLLSGKSPSGFAAAAIYAASLLCNEKKTQREVANVANVTEVTIRNRYQEQIEAMGFGV 317
>sp|P50387|TF2B_SULSH TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG (TFIIB)
>gi|11277776|pir||T46894 transcription factor TFIIB
homolog TFB [imported] - Sulfolobus shibatae
>gi|836952|gb|AAA81380.1| (U20899) transcription factor
TFIIB homolog [Sulfolobus shibatae]
Length = 309
Score = 299 bits (758), Expect = 2e-80
Identities = 153/295 (51%), Positives = 207/295 (69%), Gaps = 8/295 (2%)
Query: 10 CGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILLHDK 69
C + I+D ERGE +C+ G V+E+ I+D GPEWRAF ++EKRSR G P + +HD+
Sbjct: 15 CPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDR 74
Query: 70 GLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQL 123
GLST I + R+L R + R RKWQ R R+ + +RNLA A++EL+RI L
Sbjct: 75 GLSTLIDWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLL 134
Query: 124 KLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEK 183
LPK V++EAA +YR+AV KGL+RGRSIESV+AA +YAACR +K+ RTLDEI+ + +
Sbjct: 135 NLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAAAIYAACRRMKLARTLDEIAQYTKANR 194
Query: 184 KEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTS 243
KE+ R YR + R L+++ P DYV + A+ LGLS +V + A EI+++A GLT+
Sbjct: 195 KEVARCYRLLLRELDVSVP--VSDPKDYVTRIANLLGLSGRVMKTAAEIIDKAKGSGLTA 252
Query: 244 GKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP 298
GK PAGL AAA+YIASLL E+RTQ+E+A+VA VTEVTVRNRYKEL ++L I +P
Sbjct: 253 GKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIP 307
>gb|AAK40772.1| Transcription initiation factor IIB (TFIIB) homolog (TFB-1)
[Sulfolobus solfataricus]
Length = 309
Score = 298 bits (755), Expect = 5e-80
Identities = 153/295 (51%), Positives = 206/295 (68%), Gaps = 8/295 (2%)
Query: 10 CGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILLHDK 69
C + I+D ERGE +C+ G V+E+ I+D GPEWRAF ++EKRSR G P + +HD+
Sbjct: 15 CPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDR 74
Query: 70 GLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQL 123
GLST I + R+L R + R RKWQ R R+ + +RNLA A++EL+RI L
Sbjct: 75 GLSTLIDWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLL 134
Query: 124 KLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEK 183
LPK V++EAA +YR+AV KGL+RGRSIESV+AA +YAACR +K+ RTLDEI+ + +
Sbjct: 135 NLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAAAIYAACRRMKLARTLDEIAQYTKANR 194
Query: 184 KEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTS 243
KE+ R YR + R L+++ P DYV + A+ LGLS V + A EI+++A GLT+
Sbjct: 195 KEVARCYRLLLRELDVSVP--VSDPKDYVTRIANLLGLSGAVMKTAAEIIDKAKGSGLTA 252
Query: 244 GKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP 298
GK PAGL AAA+YIASLL E+RTQ+E+A+VA VTEVTVRNRYKEL ++L I +P
Sbjct: 253 GKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIP 307
>gb|AAF18139.1|AF205429_1 (AF205429) archaeal transcription factor B [Sulfolobus
acidocaldarius]
Length = 309
Score = 295 bits (747), Expect = 4e-79
Identities = 153/296 (51%), Positives = 203/296 (67%), Gaps = 8/296 (2%)
Query: 9 VCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILLHD 68
+C + I+D ERGE +C G VIEE I+D GPEWRAF ++EKRSR G P + +HD
Sbjct: 12 LCPPDKIIFDEERGEYICTETGEVIEERIIDQGPEWRAFTPEEKEKRSRVGGPLNQTIHD 71
Query: 69 KGLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQ 122
G+ST I + RSL R ++ R RKWQ R R+ + +RNLA A++EL+RI
Sbjct: 72 MGISTVIDWKDKDAMGRSLDPKRRLEVLRWRKWQIRTRIQSSIDRNLAQAMNELERIGNL 131
Query: 123 LKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVE 182
L LPK V++EAA +YR+AV KGL+RGRSIESV+AA +YAACR +K+ RTLDEI+ +
Sbjct: 132 LNLPKAVKDEAALIYRKAVEKGLVRGRSIESVVAASIYAACRRMKMARTLDEIAQFTKAN 191
Query: 183 KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLT 242
+KE+ R YR I R L++ + P DYV + L LS + A EI+E+A GLT
Sbjct: 192 RKEVARCYRLILRELDI--EVPVSDPKDYVTRIGTLLSLSGITMKHAAEIIEKAKNSGLT 249
Query: 243 SGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP 298
+GK PAGL AAA+YIA+LL E+RTQ+E+A+VA VTEVTVRNRYKEL ++L I++P
Sbjct: 250 AGKDPAGLAAAAIYIAALLNDERRTQKEIAQVAGVTEVTVRNRYKELTQELKIQIP 305
>emb|CAC12074.1| (AL445066) transcription initiation factor IIB related protein
[Thermoplasma acidophilum]
Length = 307
Score = 294 bits (744), Expect = 1e-78
Identities = 145/297 (48%), Positives = 208/297 (69%), Gaps = 8/297 (2%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP C S I D E GE++CA CG VIE+ +D GPEWRAFD+ Q E+R+RTG+P + L
Sbjct: 11 CPECNSEHLIRDYEHGELICADCGAVIEDAYIDQGPEWRAFDSDQDERRARTGSPMTYLS 70
Query: 67 HDKGLSTDIG------IDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
HDKGL+T+I + + R ++YR+RKW R+RVS+AAERNL+ AL L+ I
Sbjct: 71 HDKGLATEISWSNKDYYGKRIPHKNRAQIYRVRKWHQRIRVSNAAERNLSLALQLLNDIG 130
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
A+L +PK ++E AA +YR+AV K LIRGRSIES++ A +YAACR + +PRTLDEIS +
Sbjct: 131 AKLGIPKDIKETAALIYRKAVEKNLIRGRSIESIVCASIYAACRKVNIPRTLDEISKASE 190
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
V KK+IG++YR +A+ L+L K P Y+++F ++L L ++ + +I+ +A G
Sbjct: 191 VNKKKIGKAYRHLAKELDLNLKP--TTPFSYISQFCNKLDLDKQAIVISEDIVRQAMSMG 248
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKV 297
++SGK P G+ AAA+YIAS+ G+ RTQ+E+A ++ VTEVT+RNRYKE+ + L I +
Sbjct: 249 ISSGKGPTGIAAAAIYIASVKVGKPRTQKEIARISGVTEVTIRNRYKEISKALNISI 305
>gb|AAG18894.1| (AE004992) transcription initiation factor IIB; TfbF [Halobacterium
sp. NRC-1]
Length = 319
Score = 289 bits (733), Expect = 2e-77
Identities = 150/291 (51%), Positives = 197/291 (67%), Gaps = 9/291 (3%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP CG + D E GE VCA CG V+EE+ +D GPEWRAFD+ +++++SR GAP + +
Sbjct: 25 CPECGGL-VVNDEEHGESVCADCGLVVEEDGIDRGPEWRAFDSKEKDEKSRVGAPTTNTM 83
Query: 67 HDKGLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
HDKGLST+I SL+ R+KM RLRKW R R DA ERNL AL E+DR+
Sbjct: 84 HDKGLSTNIDWRDKDAYGNSLSSNQRQKMQRLRKWNERFRTRDAKERNLKQALGEIDRMA 143
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
+ L LP +V E A+ +YR A+ L+ GRSIE V +CVYAA R VPR+LDEI+D++R
Sbjct: 144 SALGLPDNVRETASVIYRRALEDDLLPGRSIEGVATSCVYAAARQAGVPRSLDEIADVSR 203
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
VEK EI R+YR++ R L L P YV +FA +LGLS++ RA E+L+ A +G
Sbjct: 204 VEKAEIARTYRYVVRELGLEVAP--ADPESYVPRFASDLGLSDEASHRARELLKTAKDKG 261
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVE 291
+ SGKSP GL AAA+Y A+LL EK TQ +V+EVA ++EVT+RNRY EL+E
Sbjct: 262 VHSGKSPVGLAAAAVYAAALLTNEKTTQAKVSEVADISEVTIRNRYHELLE 312
>sp|Q58192|TF2B_METJA TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG (TFIIB)
>gi|2129309|pir||F64397 transcription initiation factor
IIB homolog (intein-containing) - Methanococcus
jannaschii >gi|1591481|gb|AAB98771.1| (U67522)
transcription initiation factor IIB (TFIIB)
[Methanococcus jannaschii]
Length = 673
Score = 286 bits (723), Expect = 3e-76
Identities = 152/240 (63%), Positives = 186/240 (77%), Gaps = 12/240 (5%)
Query: 66 LHDKGLSTDI------GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRI 119
+HDKGLST I + L+ R ++YRLRKWQ R+RVSDAAERNLAFALSELDRI
Sbjct: 436 IHDKGLSTVIDWRNKDSYGKDLSANKRAQLYRLRKWQRRIRVSDAAERNLAFALSELDRI 495
Query: 120 TAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIA 179
T++L LP+HV E AA +YR AV KGLIRGRSIE V+AA +YAACR +VPRTLDEI++ +
Sbjct: 496 TSKLGLPRHVRENAAIIYRGAVEKGLIRGRSIEGVVAAAIYAACRRCRVPRTLDEIAEAS 555
Query: 180 RVEKKEIGRSYRFIARNLN--LTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAY 237
RV++KEIGR+YRF+AR LN LTP P DYV +FA ELGL +V +AI+IL++A
Sbjct: 556 RVDRKEIGRTYRFLARELNIKLTP----TNPIDYVPRFASELGLPGEVESKAIQILQQAA 611
Query: 238 KRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKV 297
++GLTSG+ P G+ AAA+YIAS+L G +RTQREVAEVA VTEVT+RNRYKEL E L I V
Sbjct: 612 EKGLTSGRGPTGVAAAAIYIASVLLGCRRTQREVAEVAGVTEVTIRNRYKELTEHLDIDV 671
Score = 84.3 bits (205), Expect = 2e-15
Identities = 36/56 (64%), Positives = 43/56 (76%)
Query: 6 VCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAP 61
VCP+CGS E + D ER EIVCA+CG VI+E + D+GPEWRAFD Q+ KR R GAP
Sbjct: 41 VCPICGSKEVVKDYERAEIVCAKCGCVIKEKLFDIGPEWRAFDHEQKIKRCRVGAP 96
>pir||T44261 transcription initiation factor IIB [imported] - Haloferax volcanii
>gi|5410473|gb|AAD43074.1|AF143693_1 (AF143693)
transcription factor B [Haloferax volcanii]
Length = 332
Score = 285 bits (721), Expect = 5e-76
Identities = 146/296 (49%), Positives = 203/296 (68%), Gaps = 15/296 (5%)
Query: 5 RVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESI 64
+VCP C S + D E GE +C CG V+E+ +VD GPEWRAFD+++R+ +SR GAP +
Sbjct: 35 QVCPEC-SGHLVTDEEHGETICEDCGLVVEDTVVDRGPEWRAFDSAERDSKSRVGAPTTK 93
Query: 65 LLHDKGLSTDIG------IDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDR 118
++HDKGLST+IG +SL+ RE+M RLR W R R D+ ERNL AL E+DR
Sbjct: 94 MMHDKGLSTNIGWQNKDAYGKSLSPRQREQMQRLRTWNERFRTRDSKERNLKQALGEIDR 153
Query: 119 ITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDI 178
+ + L LP++V E A+ +YR A+ L+ GRSIE V + +YA+ R+ PR+LDE+ +
Sbjct: 154 MASALGLPENVRETASVIYRRALNDDLLPGRSIEGVATSALYASARMAGTPRSLDELEKV 213
Query: 179 ARVEKKEIGRSYRFIARNLNLTPKKLFVKPTD---YVNKFADELGLSEKVRRRAIEILEE 235
+RV+K E+ R+YR+I R L KL +KP D YV +FA ELGLS++ R+A ++L +
Sbjct: 214 SRVDKMELTRTYRYIVREL-----KLEIKPADPEQYVPRFASELGLSDEAERQARQLLRD 268
Query: 236 AYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVE 291
A + G+ SGKSP GL AAA+Y A+LL EK TQ EV+ VA ++EVT+RNRYKEL+E
Sbjct: 269 AKETGIHSGKSPVGLAAAAVYAAALLTNEKVTQSEVSTVADISEVTIRNRYKELLE 324
>gb|AAG19212.1| (AE005017) transcription initiation factor IIB; TfbB [Halobacterium
sp. NRC-1]
Length = 325
Score = 277 bits (702), Expect = 8e-74
Identities = 140/291 (48%), Positives = 198/291 (67%), Gaps = 9/291 (3%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP CG + I D E GE VC CG V+EEN +D GPEWRAF++++++++SR GAP + ++
Sbjct: 32 CPECGG-QVIDDEEHGESVCVDCGLVVEENGIDRGPEWRAFNSTEKDEKSRVGAPTTNMM 90
Query: 67 HDKGLSTDIG------IDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
HDKGLST+IG SL+ R+KM RLRKW R R ++ ERNL AL E++R+
Sbjct: 91 HDKGLSTNIGWQDKDAYGNSLSSNQRQKMQRLRKWNERFRTRNSKERNLKQALGEIERMA 150
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
+ L LPK V E A+ +YR A+ + L+ GRSIE V A +YA+ R L PR++DE+++++R
Sbjct: 151 SALGLPKEVRETASVIYRRALSEDLLPGRSIEGVATAALYASARQLSTPRSIDEVANVSR 210
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
+++ E R+YR+I R L+L P YV +FA +L L ++V RR+ E++ A G
Sbjct: 211 IDEMEFKRTYRYIVRELSLEVAP--ADPAQYVPRFASDLDLPDEVERRSRELISNAQADG 268
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVE 291
+TSGKSP GL AAA+Y +SLL K TQ EV+EV V+EVT+RNRY+EL+E
Sbjct: 269 VTSGKSPVGLAAAAIYASSLLTNHKVTQSEVSEVTDVSEVTIRNRYQELLE 319
>gb|AAG19313.1| (AE005026) transcription initiation factor IIB; TfbD [Halobacterium
sp. NRC-1]
Length = 328
Score = 268 bits (679), Expect = 4e-71
Identities = 139/297 (46%), Positives = 196/297 (65%), Gaps = 15/297 (5%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP C + + D +RGE C CG V+EE+ +D GPEWRAF+A +++++SR GAP + ++
Sbjct: 32 CPECNG-DLVTDEDRGETTCGECGLVVEEDGIDHGPEWRAFNAQEQDEKSRVGAPTTNMM 90
Query: 67 HDKGLSTDIG------IDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT 120
HDKGLST+IG +L+G R KM RLRKW R R ++ ERNL AL E++R+
Sbjct: 91 HDKGLSTNIGWQDKDAYGNTLSGRQRRKMQRLRKWNERFRTRNSKERNLKQALGEIERMA 150
Query: 121 AQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR 180
+ LP V E A+ +YR A+ + L+ GRSIE V + +YAA R PR+LDE++ ++R
Sbjct: 151 SAFGLPDSVRETASVIYRRALGEDLLPGRSIEGVATSALYAAARQANTPRSLDEVASVSR 210
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTD---YVNKFADELGLSEKVRRRAIEILEEAY 237
V++ EI R+YR++AR L+L V PTD YV +F +L LS V RRA ++L A
Sbjct: 211 VDRGEIARTYRYVARELSLE-----VAPTDPASYVPRFCSDLELSGDVERRARDLLAAAA 265
Query: 238 KRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLG 294
G+TSGKSP GL AA++Y A+LL E+ TQ EV+ VA V+EVT+RNRY E+++ G
Sbjct: 266 DAGITSGKSPVGLAAASVYAAALLTNERVTQNEVSTVANVSEVTIRNRYHEILDAGG 322
>pir||D71137 probable transcription initiation factor IIB - Pyrococcus
horikoshii >gi|3257275|dbj|BAA29958.1| (AP000003) 208aa
long hypothetical transcription initiation factor IIB
[Pyrococcus horikoshii]
Length = 208
Score = 237 bits (599), Expect = 1e-61
Identities = 114/214 (53%), Positives = 162/214 (75%), Gaps = 6/214 (2%)
Query: 84 LMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRK 143
++REK++RL+ R+ + + E+ RI++QL LPKHVE EA R+YR+ ++
Sbjct: 1 MVREKIHRLK------RLDSFGNKTEKLGVEEISRISSQLCLPKHVEREAVRIYRKLIKS 54
Query: 144 GLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKK 203
G+ +GRSIESV AAC+Y +CRL KVPRTLDEI+ +A+ +KK I R YR + + L L+ K
Sbjct: 55 GVTKGRSIESVAAACIYISCRLYKVPRTLDEIAKVAKEDKKVIARVYRLVVKKLGLSSKD 114
Query: 204 LFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEG 263
+ ++P Y++KFADEL +SE+V+RRA+ +L EA +G+TSGK+P GL A+ LYIASLLEG
Sbjct: 115 MLIRPEYYIDKFADELEVSERVKRRALRLLNEAKDKGITSGKNPLGLAASILYIASLLEG 174
Query: 264 EKRTQREVAEVARVTEVTVRNRYKELVEKLGIKV 297
E+RTQ+E+A VA +TEVT+RNRYKELV++L I+V
Sbjct: 175 ERRTQKEIARVAGITEVTIRNRYKELVKELRIRV 208
>pir||B72475 probable transcription initiation factor IIB APE2443 - Aeropyrum
pernix (strain K1) >gi|5106147|dbj|BAA81458.1|
(AP000064) 325aa long hypothetical transcription
initiation factor IIB [Aeropyrum pernix]
Length = 325
Score = 193 bits (486), Expect = 2e-48
Identities = 106/288 (36%), Positives = 176/288 (60%), Gaps = 9/288 (3%)
Query: 16 IYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILLHDKGLSTDI 75
+ DP RG +CA G +I E+I+ + +A+ +R++++ G P H G+ +
Sbjct: 28 VTDPVRGLKICADTGEIIGEDIIGTESDVKAYTPEERQQKTHYGGPLKYSHHYMGVEASL 87
Query: 76 GI--DRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEEEA 133
D G+ + K+ R + R + ++NL ALS ++ + +++ +P+ V E+A
Sbjct: 88 EHPRDHGPKGIKQRKILPRRPPRLSARPLTSVDKNLQTALSLINEVASRMGMPEIVVEDA 147
Query: 134 ARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEI-----SDIARVEKKEIGR 188
+++YREA+ KGL RGRSIES++AA +YAA R+ +P +L +I ++ ++++ R
Sbjct: 148 SKIYREAMEKGLTRGRSIESIVAASLYAASRIHGLPHSLTDIIKAMKGNVDAETRRDVAR 207
Query: 189 SYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKSPA 248
SYR + R+LN+ K KP ++V LGL E V AI+I++ + K+GLT+GK P
Sbjct: 208 SYRLLVRDLNI--KIPVRKPENFVYTIISALGLPEHVAIEAIKIIDLSRKKGLTAGKDPG 265
Query: 249 GLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIK 296
GL AA+Y+A+L G ++TQ+E+A V VTEVT+RNRYKE+ + LGI+
Sbjct: 266 GLAGAAVYLAALKHGIRKTQKEIAHVVGVTEVTIRNRYKEIAQALGIE 313
>gb|AAK41221.1| Transcription initiation factor IIB (TFIIB) homolog (TFB-2)
[Sulfolobus solfataricus]
Length = 293
Score = 184 bits (462), Expect = 1e-45
Identities = 106/293 (36%), Positives = 170/293 (57%), Gaps = 6/293 (2%)
Query: 7 CPVCGSTEFI-YDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESIL 65
CP C + I YD E+G VC C VIE++ VD GP+WRA++A R ++ R G+P +
Sbjct: 3 CPYCKTDNAITYDVEKGMYVCTNCASVIEDSAVDPGPDWRAYNAKDRNEKERVGSPSTPK 62
Query: 66 LHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKL 125
+HD G T IG R+ L KM R+ Q+++RVS ++ L LS L+ +++L+L
Sbjct: 63 VHDWGFHTIIGYGRAKDRLKTLKMQRM---QNKIRVSPK-DKKLVTLLSILNDESSKLEL 118
Query: 126 PKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKE 185
P+HV+E A+ + R+ V GL + ++I A +Y +C++ +PR L E + E
Sbjct: 119 PEHVKETASLIIRKMVETGLTKRIDQYTLIVAALYYSCQVNNIPRHLQEFKVRYSISSSE 178
Query: 186 IGRSYRFIARNLNLTPK-KLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSG 244
+ + + N P + +KP +Y+ K +L L + +A E+++ +K+GLTSG
Sbjct: 179 FWSALKRVQYVANSIPGFRPKIKPAEYIPKILYKLNLPPIIGTKASELVDLMHKQGLTSG 238
Query: 245 KSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKV 297
K L AA++Y+ S L K+TQ+EVA+ +TEVT+RNRYK++V+ I V
Sbjct: 239 KGYLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIVV 291
>gb|AAG20319.1| (AE005105) transcription initiation factor IIB; TfbA [Halobacterium
sp. NRC-1]
Length = 287
Score = 182 bits (457), Expect = 5e-45
Identities = 105/285 (36%), Positives = 150/285 (51%), Gaps = 5/285 (1%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP C T D E VCA CG V+ + +D GPEWR F RTGAP +
Sbjct: 7 CPECDGTIRETDTEH---VCADCGLVVTDAPIDHGPEWRTFSDDPDHAPERTGAPLTRSR 63
Query: 67 HDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLP 126
HD+GLST+IG LTG R K RLR+ R + A+ N + +E+ R+ +
Sbjct: 64 HDRGLSTEIGYSARLTGRKRRKFARLRRQHKRATIPSKADYNRIYGFTEIRRLIGTFGIG 123
Query: 127 KHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEI 186
+ ++A L+ A GL+ GR+IE AA VYA CR V RTLDEI+ AR + E+
Sbjct: 124 TGLRDQACTLFASAQTAGLLHGRTIEGFAAAGVYATCRTNGVTRTLDEIAAAARATRPEL 183
Query: 187 GRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKS 246
+Y + R+L L + P +Y+ ++AD L L ++ R+A E++ GL +G++
Sbjct: 184 TTAYDAMNRDLGLPTGP--IDPREYLPRYADALSLPARIERQARELVAALETDGLVAGRN 241
Query: 247 PAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVE 291
P+G+ A LY A+ TQ A VA VT VT+R Y+ L +
Sbjct: 242 PSGVAAGCLYTAATETDHAVTQSAAAAVADVTPVTLRATYQNLTD 286
>gb|AAG21002.1| (AE005167) transcription initiation factor IIB; TfbE [Halobacterium
sp. NRC-1]
Length = 307
Score = 174 bits (436), Expect = 1e-42
Identities = 106/290 (36%), Positives = 158/290 (53%), Gaps = 15/290 (5%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP CG E VCA CG +I+E +D GPEW DA + RTGAP +
Sbjct: 23 CPECGGP---VRTNSAETVCADCGLIIDEQSIDRGPEWHRDDA---DTAKRTGAPLTPAR 76
Query: 67 HDKGLSTDIGIDRSLTGLM-----REKMYRLRKWQSRLRVSDAAERNLAFALSELDRITA 121
HD+GLST IG + G R ++ R+R+ QSR R ERNL L+E+ RI +
Sbjct: 77 HDRGLSTVIGSGQDARGTALSSEKRRRLARMRREQSRGRWRSTKERNLGHGLTEIRRIAS 136
Query: 122 QLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARV 181
L L V ++A +L+R A + L++GRSIE++ AA V+ ACR +D+++ +A+V
Sbjct: 137 ALGLADSVRDQACQLFRTAQNERLLKGRSIEAMAAASVFGACRCNGESWLIDDVAPMAQV 196
Query: 182 EKKEIGRSYRFIARNLNL-TPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
+ + +Y + L L TP V +V + A +LG ++ VRRRA E++ +A G
Sbjct: 197 GQNRVENAYTVLNEELGLPTPP---VSLDQFVPRLASDLGCTDVVRRRAEELVVQAVDAG 253
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELV 290
+T+G P+G AA LY+A+ + TQ + A A VT T+R+ L+
Sbjct: 254 ITTGVHPSGFAAACLYMAACVHDAPLTQADAAAAAGVTVETIRSHRDRLL 303
>gi|4504193 general transcription factor IIB [Homo sapiens]
>gi|135629|sp|Q00403|TF2B_HUMAN TRANSCRIPTION INITIATION
FACTOR IIB (TFIIB) >gi|2144953|pir||TWHU2B transcription
initiation factor IIB [validated] - human
>gi|37058|emb|CAA41958.1| (X59268) IIB protein [Homo
sapiens] >gi|339490|gb|AAA61149.1| (M76766)
transcription factor [Homo sapiens]
>gi|228040|prf||1715332A transcription initiation factor
IIB [Homo sapiens]
Length = 316
Score = 169 bits (423), Expect = 4e-41
Identities = 91/282 (32%), Positives = 158/282 (55%), Gaps = 7/282 (2%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKR-SRTGAPESIL 65
CP + D G+++C CG V+ + ++D+G EWR F + K SR G ++ L
Sbjct: 15 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPL 74
Query: 66 LHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKL 125
L D LST IG TG + K+Q+R R +++R + A E+ + ++ L
Sbjct: 75 LSDGDLSTMIGKG---TGAASFDEFGNSKYQNR-RTMSSSDRAMMNAFKEITTMADRINL 130
Query: 126 PKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKE 185
P+++ + L+++ + ++GR+ +++ +AC+Y ACR VPRT EI ++R+ KKE
Sbjct: 131 PRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE 190
Query: 186 IGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGK 245
IGR ++ I + L + + D++++F L L ++V+ A I +A + L G+
Sbjct: 191 IGRCFKLILKALETSVD--LITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGR 248
Query: 246 SPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYK 287
SP + AAA+Y+AS EKRTQ+E+ ++A V +VT+R Y+
Sbjct: 249 SPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYR 290
>sp|P29053|TF2B_RAT TRANSCRIPTION INITIATION FACTOR IIB (TFIIB) (RNA POLYMERASE II
ALPHA INITIATION FACTOR) >gi|111357|pir||S26299
transcription initiation factor IIB - rat
>gi|55624|emb|CAA46766.1| (X65948) alpha initiation
factor [Rattus norvegicus]
Length = 316
Score = 169 bits (423), Expect = 4e-41
Identities = 91/282 (32%), Positives = 158/282 (55%), Gaps = 7/282 (2%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKR-SRTGAPESIL 65
CP + D G+++C CG V+ + ++D+G EWR F + K SR G ++ L
Sbjct: 15 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPL 74
Query: 66 LHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKL 125
L D LST IG TG + K+Q+R R +++R + A E+ + ++ L
Sbjct: 75 LSDGDLSTMIGKG---TGAASFDEFGNSKYQNR-RTMSSSDRAMMNAFKEITTMADRINL 130
Query: 126 PKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKE 185
P+++ + L+++ + ++GR+ +++ +AC+Y ACR VPRT EI ++R+ KKE
Sbjct: 131 PRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE 190
Query: 186 IGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGK 245
IGR ++ I + L + + D++++F L L ++V+ A I +A + L G+
Sbjct: 191 IGRCFKLILKALETSVD--LITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGR 248
Query: 246 SPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYK 287
SP + AAA+Y+AS EKRTQ+E+ ++A V +VT+R Y+
Sbjct: 249 SPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYR 290
>gb|AAB23144.1| (S44184) S300-II, TFIIB=transcription factor [human, Peptide
Partial, 311 aa] [Homo sapiens]
Length = 311
Score = 169 bits (423), Expect = 4e-41
Identities = 91/282 (32%), Positives = 158/282 (55%), Gaps = 7/282 (2%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKR-SRTGAPESIL 65
CP + D G+++C CG V+ + ++D+G EWR F + K SR G ++ L
Sbjct: 10 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPL 69
Query: 66 LHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKL 125
L D LST IG TG + K+Q+R R +++R + A E+ + ++ L
Sbjct: 70 LSDGDLSTMIGKG---TGAASFDEFGNSKYQNR-RTMSSSDRAMMNAFKEITTMADRINL 125
Query: 126 PKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKE 185
P+++ + L+++ + ++GR+ +++ +AC+Y ACR VPRT EI ++R+ KKE
Sbjct: 126 PRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKE 185
Query: 186 IGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGK 245
IGR ++ I + L + + D++++F L L ++V+ A I +A + L G+
Sbjct: 186 IGRCFKLILKALETSVD--LITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGR 243
Query: 246 SPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYK 287
SP + AAA+Y+AS EKRTQ+E+ ++A V +VT+R Y+
Sbjct: 244 SPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYR 285
>sp|P29054|TF2B_XENLA TRANSCRIPTION INITIATION FACTOR IIB (TFIIB) >gi|104198|pir||S20071
transcription initiation factor IIB - African clawed
frog >gi|65129|emb|CAA44668.1| (X62868) TFIIB [Xenopus
laevis]
Length = 316
Score = 168 bits (422), Expect = 6e-41
Identities = 90/282 (31%), Positives = 159/282 (55%), Gaps = 7/282 (2%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKR-SRTGAPESIL 65
CP + D G+++C+ CG V+ + ++D+G EWR F + SR G ++ L
Sbjct: 15 CPNHPDALLVEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNDKAAADPSRVGDAQNPL 74
Query: 66 LHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKL 125
L L+T IG TG + K+Q+R R +++R + A E+ ++ ++ L
Sbjct: 75 LSGGDLTTMIGKG---TGSASFDEFGNSKYQNR-RTMSSSDRAMMNAFKEITNMSDRINL 130
Query: 126 PKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKE 185
P+++ + L+++ + ++GRS +++ +AC+Y ACR VPRT EI ++R+ KKE
Sbjct: 131 PRNIIDRTNNLFKQVYEQKSLKGRSNDAIASACLYIACRQEGVPRTFKEICAVSRISKKE 190
Query: 186 IGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGK 245
IGR ++ I + L + D++++F LGL+++V+ A I +A + L G+
Sbjct: 191 IGRCFKLILKALETNVD--LITTGDFMSRFCSNLGLTKQVQMAATHIARKAVELDLVPGR 248
Query: 246 SPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYK 287
SP + AAA+Y+AS EKRTQ+E+ ++A V +VT+R Y+
Sbjct: 249 SPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYR 290
>sp|P29052|TF2B_DROME TRANSCRIPTION INITIATION FACTOR IIB (TFIIB) >gi|539450|pir||A42695
transcription initiation factor IIB - fruit fly
(Drosophila melanogaster) >gi|158538|gb|AAA28929.1|
(M91081) RNA polymerase II transcription factor
[Drosophila melanogaster] >gi|158540|gb|AAA28930.1|
(M88164) transcription factor IIB [Drosophila
melanogaster] >gi|414282|gb|AAA68626.1| (U02879)
transcription factor IIB [Drosophila melanogaster]
>gi|1016268|gb|AAA79093.1| (U35148) transcription factor
IIB [Drosophila melanogaster] >gi|7297699|gb|AAF52951.1|
(AE003628) TfIIB gene product [Drosophila melanogaster]
Length = 315
Score = 163 bits (408), Expect = 3e-39
Identities = 92/282 (32%), Positives = 154/282 (53%), Gaps = 7/282 (2%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQRE-KRSRTGAPESIL 65
C + I D G+++C+ CG V+ + ++D+G EWR F + SR G PE+ L
Sbjct: 14 CYAHPESPLIEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNEKSGVDPSRVGGPENPL 73
Query: 66 LHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKL 125
L LST IG TG + K+Q+R R +++R+L A E+ + ++ L
Sbjct: 74 LSGGDLSTIIGPG---TGSASFDAFGAPKYQNR-RTMSSSDRSLISAFKEISSMADRINL 129
Query: 126 PKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKE 185
PK + + A L+++ ++GRS ++ +AC+Y ACR VPRT EI ++++ KKE
Sbjct: 130 PKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKE 189
Query: 186 IGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGK 245
IGR ++ + L + + D++ +F L L V+R A I ++A + + G+
Sbjct: 190 IGRCFKLTLKALETSVD--LITTADFMCRFCANLDLPNMVQRAATHIAKKAVEMDIVPGR 247
Query: 246 SPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYK 287
SP + AAA+Y+AS KR+Q+E+ ++A V +VT+R YK
Sbjct: 248 SPISVAAAAIYMASQASEHKRSQKEIGDIAGVADVTIRQSYK 289
>gi|11425425 general transcription factor IIB [Homo sapiens]
Length = 278
Score = 150 bits (376), Expect = 1e-35
Identities = 83/254 (32%), Positives = 145/254 (56%), Gaps = 7/254 (2%)
Query: 35 ENIVDMGPEWRAFDASQREKR-SRTGAPESILLHDKGLSTDIGIDRSLTGLMREKMYRLR 93
+ ++D+G EWR F + K SR G ++ LL D LST IG TG +
Sbjct: 5 DRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKG---TGAASFDEFGNS 61
Query: 94 KWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIES 153
K+Q+R R +++R + A E+ + ++ LP+++ + L+++ + ++GR+ ++
Sbjct: 62 KYQNR-RTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDA 120
Query: 154 VIAACVYAACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVN 213
+ +AC+Y ACR VPRT EI ++R+ KKEIGR ++ I + L + + D+++
Sbjct: 121 IASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVD--LITTGDFMS 178
Query: 214 KFADELGLSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAE 273
+F L L ++V+ A I +A + L G+SP + AAA+Y+AS EKRTQ+E+ +
Sbjct: 179 RFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGD 238
Query: 274 VARVTEVTVRNRYK 287
+A V +VT+R Y+
Sbjct: 239 IAGVADVTIRQSYR 252
>emb|CAA84309.1| (Z34661) AtTFIIB2 [Arabidopsis thaliana]
Length = 301
Score = 143 bits (356), Expect = 3e-33
Identities = 91/292 (31%), Positives = 150/292 (51%), Gaps = 13/292 (4%)
Query: 13 TEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAF-DASQREKRSRTGAPESILLHDKGL 71
TE ++D G+ VC+ CG V+E + +D EWR F + S R G P + LL D GL
Sbjct: 2 TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGL 61
Query: 72 STDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEE 131
+T I +G L +WQ+R +R L A + + +L L +++
Sbjct: 62 TTVISKPNGSSGDFLSSS--LGRWQNR---GSNPDRGLIVAFKTIATMADRLGLVATIKD 116
Query: 132 EAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR-VEKKEIGRSY 190
A +Y+ + RGR+ ++++AAC+Y ACR PRT+ EI +A KKEIGR+
Sbjct: 117 RANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAK 176
Query: 191 RFIARNLNLTPKKLFVKPT----DYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKS 246
+I + L L +L T D++ +F LG++ + + A E ++++ + + +S
Sbjct: 177 EYIVKQLGLETGQLVEMGTIHAGDFMRRFCSNLGMTNQTVKAAQESVQKSEEFDIR--RS 234
Query: 247 PAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP 298
P + AA +YI + L EK+ R+++ V E T+RN YK+L L +P
Sbjct: 235 PISIAAAVIYIITQLSDEKKPLRDISVATGVAEGTIRNSYKDLYPHLSKIIP 286
>gb|AAF02810.1|AC009400_6 (AC009400) transcription initiation factor IIB (TFIIB) [Arabidopsis
thaliana]
Length = 312
Score = 143 bits (356), Expect = 3e-33
Identities = 91/292 (31%), Positives = 150/292 (51%), Gaps = 13/292 (4%)
Query: 13 TEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAF-DASQREKRSRTGAPESILLHDKGL 71
TE ++D G+ VC+ CG V+E + +D EWR F + S R G P + LL D GL
Sbjct: 13 TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGL 72
Query: 72 STDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEE 131
+T I +G L +WQ+R +R L A + + +L L +++
Sbjct: 73 TTVISKPNGSSGDFLSSS--LGRWQNR---GSNPDRGLIVAFKTIATMADRLGLVATIKD 127
Query: 132 EAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR-VEKKEIGRSY 190
A +Y+ + RGR+ ++++AAC+Y ACR PRT+ EI +A KKEIGR+
Sbjct: 128 RANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAK 187
Query: 191 RFIARNLNLTPKKLFVKPT----DYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKS 246
+I + L L +L T D++ +F LG++ + + A E ++++ + + +S
Sbjct: 188 EYIVKQLGLETGQLVEMGTIHAGDFMRRFCSNLGMTNQTVKAAQESVQKSEEFDIR--RS 245
Query: 247 PAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP 298
P + AA +YI + L EK+ R+++ V E T+RN YK+L L +P
Sbjct: 246 PISIAAAVIYIITQLSDEKKPLRDISVATGVAEGTIRNSYKDLYPHLSKIIP 297
>sp|P48512|TF2B_ARATH TRANSCRIPTION INITIATION FACTOR IIB (TFIIB) >gi|7443550|pir||T00819
transcription initiation factor IIB - Arabidopsis
thaliana >gi|945085|gb|AAB09755.1| (U31096)
transcription factor TFIIB [Arabidopsis thaliana]
>gi|2618697|gb|AAB84344.1| (AC002510) transcription
factor IIB (TFIIB) [Arabidopsis thaliana]
Length = 312
Score = 138 bits (344), Expect = 8e-32
Identities = 90/292 (30%), Positives = 147/292 (49%), Gaps = 13/292 (4%)
Query: 13 TEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAF-DASQREKRSRTGAPESILLHDKGL 71
TE + D G+ +C+ CG V+E + +D EWR F + S +R G P + LL D L
Sbjct: 13 TELVVDHSAGDTLCSECGLVLESHSIDETSEWRTFANESSNSDPNRVGGPTNPLLADSAL 72
Query: 72 STDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEE 131
+T I +G L +WQ+R SD R L A + ++ +L L +++
Sbjct: 73 TTVIAKPNGSSGDFLSSS--LGRWQNRNSNSD---RGLIQAFKTIATMSERLGLVATIKD 127
Query: 132 EAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR-VEKKEIGRSY 190
A LY+ + RGR+ +++ AAC+Y ACR PRT+ EI IA KKEIGR+
Sbjct: 128 RANELYKRLEDQKSSRGRNQDALYAACLYIACRQEDKPRTIKEICVIANGATKKEIGRAK 187
Query: 191 RFIARNLNLTPKKLF----VKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKS 246
+I + L L P + + D++ +F L +S + A E ++++ + + +S
Sbjct: 188 DYIVKTLGLEPGQSVDLGTIHAGDFMRRFCSNLAMSNHAVKAAQEAVQKSEEFDIR--RS 245
Query: 247 PAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP 298
P + A +YI + L +K+T ++++ V E T+RN YK+L L P
Sbjct: 246 PISIAAVVIYIITQLSDDKKTLKDISHATGVAEGTIRNSYKDLYPHLSKIAP 297
>pir||T37796 probable transcription initiation factor TFIIB subunit - fission
yeast (Schizosaccharomyces pombe)
>gi|2330704|emb|CAB11044.1| (Z98529) transcription
initiation factor iib [Schizosaccharomyces pombe]
Length = 340
Score = 138 bits (344), Expect = 8e-32
Identities = 98/308 (31%), Positives = 148/308 (47%), Gaps = 27/308 (8%)
Query: 1 MTKQRVCPVCGST--EFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKR--S 56
++ + +C C + + G+ VC CG V+ + I+D EWR F S S
Sbjct: 14 LSVKMICSECREDPPNLVEEFSSGDTVCGSCGLVLGDRIIDTRSEWRTFSNSDEASGDPS 73
Query: 57 RTGAPESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSEL 116
R G + LL+ L T I G M L K Q R V E+NL A E+
Sbjct: 74 RVGKVANPLLNGSQLDTTIS-SYDGAGAM------LAKAQGR-SVQVRGEKNLLTAYKEI 125
Query: 117 DRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEIS 176
+ + LPK + + A +LY+ ++G+S +S+IAAC+Y ACR KVPRT EI
Sbjct: 126 GAMCDAISLPKVIADTAKQLYKRVDDHKALKGKSSQSIIAACIYIACRQGKVPRTFMEIC 185
Query: 177 DIARVEKKEIGRSYRFIARNLN----LTPKKLFVKPTDYVN-----------KFADELGL 221
+ V KKEIGR Y+ + R L L +K +Y+ +F + L L
Sbjct: 186 TLTNVPKKEIGRVYKTLQRMLTEGGALHNSVDALKGHEYIQSSSTSAEDLMVRFCNRLML 245
Query: 222 SEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVT 281
V+ A E+ A +G +G+SP + A+ +Y+ S L G +T +E++EV V++ T
Sbjct: 246 PMSVQSAAAELARRAGLQGTLAGRSPISIAASGIYMISALMGYPKTPKEISEVTGVSDST 305
Query: 282 VRNRYKEL 289
+R YK L
Sbjct: 306 IRIAYKLL 313
>gb|AAF71709.1| (AF220260) transcription factor TFIIB [Drosophila virilis]
Length = 293
Score = 132 bits (330), Expect = 4e-30
Identities = 85/281 (30%), Positives = 143/281 (50%), Gaps = 27/281 (9%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
C + I D G+++C+ CG V+ + AF G PE+ LL
Sbjct: 14 CYAHPESPLIEDYRAGDMICSECGLVVGD----------AF-----------GGPENPLL 52
Query: 67 HDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLP 126
LST IG TG + K+ +R R +++R+L A E+ + ++ LP
Sbjct: 53 SGGHLSTIIGPG---TGSASFDAFGAPKYPNR-RTMRSSDRSLISAFKEISSMADRINLP 108
Query: 127 KHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEI 186
K + + A L+++ ++GRS ++ +AC+Y ACR VPRT EI ++++ KKEI
Sbjct: 109 KTIVDRANTLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSKISKKEI 168
Query: 187 GRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKS 246
GR ++ + L + + D++ +F L L V+R A I ++A + + G+S
Sbjct: 169 GRCFKLTLKALETSVD--LITTADFMCRFCANLDLPNMVQRAATHIAKKAVEMDIVPGRS 226
Query: 247 PAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYK 287
P + AAA+Y+AS KR+Q+E+ ++A V +VT+R YK
Sbjct: 227 PISVAAAAIYMASQASEHKRSQKEIGDIAGVADVTIRQSYK 267
>sp|P48513|TF2B_SOYBN TRANSCRIPTION INITIATION FACTOR IIB (TFIIB) >gi|7443551|pir||T06440
probable transcription initiation factor TFIIB - soybean
>gi|945087|gb|AAB09756.1| (U31097) transcription factor
TFIIB [Glycine max]
Length = 313
Score = 131 bits (326), Expect = 1e-29
Identities = 87/292 (29%), Positives = 148/292 (49%), Gaps = 12/292 (4%)
Query: 13 TEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAF-DASQREKRSRTGAPESILLHDKGL 71
TE ++D G+ VC+ CG V+E + +D EWR F + S +R G P + LL D GL
Sbjct: 13 TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPNRVGGPSNPLLTDGGL 72
Query: 72 STDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEE 131
ST I G L +WQ+R +R L A + ++ +L L +++
Sbjct: 73 STVIAKPNGGGG-GEFLSSSLGRWQNR---GSNPDRALIQAFKTIATMSDRLGLVATIKD 128
Query: 132 EAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR-VEKKEIGRSY 190
A +Y+ + RGR+ ++++AAC+Y ACR PRT+ EI +A KKEIGR+
Sbjct: 129 RANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAK 188
Query: 191 RFIARNLNLTPKKLFVKPT----DYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKS 246
+I + L L T D++ +F L ++ + + A E ++++ + + +S
Sbjct: 189 EYIVKQLGLENGNAVEMGTIHAGDFMRRFCSNLCMNNQAVKAAQEAVQKSEEFDIR--RS 246
Query: 247 PAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVP 298
P + AA +YI + L +K+ ++++ V E T+RN YK+L + +P
Sbjct: 247 PISIAAAVIYIITQLSDDKKPLKDISLATGVAEGTIRNSYKDLYPHVSKIIP 298
>sp|P42198|TF2B_HALVA TRANSCRIPTION INITIATION FACTOR IIB HOMOLOG (TFIIB)
>gi|1364140|pir||S55298 transcription initiation factor
IIB - Haloarcula vallismortis (fragment)
>gi|516324|emb|CAA84551.1| (Z35308) transcription factor
IIB homolog [Haloarcula vallismortis]
Length = 114
Score = 127 bits (316), Expect = 2e-28
Identities = 66/115 (57%), Positives = 88/115 (76%), Gaps = 2/115 (1%)
Query: 181 VEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
VE+KEIGR+YR++A+ L L + V P YV +FA EL LSE+V+ +A EI++ ++G
Sbjct: 1 VEQKEIGRTYRYVAQELELKMEP--VDPKQYVPRFASELELSEEVQSKANEIIDTTAEQG 58
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGI 295
L SGKSP G AAA+Y ASLL EK+TQREVA+VA+VTEVT+RNRY+E +E +GI
Sbjct: 59 LLSGKSPTGYAAAAIYAASLLCNEKKTQREVADVAQVTEVTIRNRYQEQIEAMGI 113
>sp|Q05959|TF2B_KLULA TRANSCRIPTION INITIATION FACTOR IIB (TFIIB) >gi|479623|pir||S34953
transcription initiation factor IIB - yeast
(Kluyveromyces marxianus var. lactis)
>gi|173289|gb|AAA35258.1| (L11606) human TFIIB homologue
protein [Kluyveromyces lactis]
Length = 357
Score = 125 bits (312), Expect = 5e-28
Identities = 90/305 (29%), Positives = 148/305 (48%), Gaps = 28/305 (9%)
Query: 7 CPVCGSTEFIYDPE------RGEIVCARCGYVIEENIVDMGPEWRAF--DASQREKRSRT 58
CP C +Y P+ G+IVCA CG V+ + IVD EWR F D + SR
Sbjct: 28 CPECK----VYPPKVVERFSEGDIVCALCGLVLSDRIVDTRSEWRTFSNDDQNGDDPSRV 83
Query: 59 GAPESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDR 118
G + LL L+T IG + + + R QS+ + D + L A +++
Sbjct: 84 GEASNPLLDGNHLTTRIGQAQGSDARVTRDLNRA---QSK-SIVDKKDNELQAAYAKITM 139
Query: 119 ITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDI 178
+ +LPK V++ A Y+ + +++G+S ES++A+ + CR +V R+ EI +
Sbjct: 140 MCDAAELPKIVKDCAKEAYKLCFEERVLKGKSQESIMASVILVGCRRAEVGRSFKEILSL 199
Query: 179 ARVEKKEIGRSYRFIARNL---------NLTPKKLFVKPTD---YVNKFADELGLSEKVR 226
V KKEIG+++ I L N+ + T ++ +F LGLS +V
Sbjct: 200 TNVRKKEIGKTFTIIKNILREKNDRGFANIDTSNITTGQTSAETFIPRFCSHLGLSVQVA 259
Query: 227 RRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRY 286
A I + + + +G+SP + AAA+Y+A+LL + +++ +VTE TV+ Y
Sbjct: 260 NAAEYIAKHSKDVNVLAGRSPITIAAAAIYMATLLFKLNISPTRISQTLQVTEGTVKGGY 319
Query: 287 KELVE 291
K L E
Sbjct: 320 KALYE 324
>pdb|1TFB| Nmr Studies Of Human General Transcription Factor Tfiib: Dynamics
And Interaction With Vp16 Activation Domain, 20
Structures
Length = 208
Score = 124 bits (308), Expect = 1e-27
Identities = 60/183 (32%), Positives = 109/183 (58%), Gaps = 2/183 (1%)
Query: 105 AERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACR 164
A R + A E+ + ++ LP+++ + L+++ + ++GR+ +++ +AC+Y ACR
Sbjct: 2 ASRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACR 61
Query: 165 LLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK 224
VPRT EI ++R+ KKEIGR ++ I + L + + D++++F L L ++
Sbjct: 62 QEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVD--LITTGDFMSRFCSNLCLPKQ 119
Query: 225 VRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRN 284
V+ A I +A + L G+SP + AAA+Y+AS EKRTQ+E+ ++A V +VT+R
Sbjct: 120 VQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQ 179
Query: 285 RYK 287
Y+
Sbjct: 180 SYR 182
>pdb|1C9B|A Chain A, Crystal Structure Of A Human Tbp Core Domain-Human Tfiib
Core Domain Complex Bound To An Extended, Modified
Adenoviral Major Late Promoter (Admlp)
>gi|6980709|pdb|1C9B|E Chain E, Crystal Structure Of A
Human Tbp Core Domain-Human Tfiib Core Domain Complex
Bound To An Extended, Modified Adenoviral Major Late
Promoter (Admlp) >gi|6980711|pdb|1C9B|I Chain I, Crystal
Structure Of A Human Tbp Core Domain-Human Tfiib Core
Domain Complex Bound To An Extended, Modified Adenoviral
Major Late Promoter (Admlp) >gi|6980713|pdb|1C9B|M Chain
M, Crystal Structure Of A Human Tbp Core Domain-Human
Tfiib Core Domain Complex Bound To An Extended, Modified
Adenoviral Major Late Promoter (Admlp)
>gi|6980715|pdb|1C9B|Q Chain Q, Crystal Structure Of A
Human Tbp Core Domain-Human Tfiib Core Domain Complex
Bound To An Extended, Modified Adenoviral Major Late
Promoter (Admlp)
Length = 207
Score = 123 bits (307), Expect = 2e-27
Identities = 59/183 (32%), Positives = 110/183 (59%), Gaps = 2/183 (1%)
Query: 105 AERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACR 164
++R + A E+ + ++ LP+++ + L+++ + ++GR+ +++ +AC+Y ACR
Sbjct: 1 SDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACR 60
Query: 165 LLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK 224
VPRT EI ++R+ KKEIGR ++ I + L + + D++++F L L ++
Sbjct: 61 QEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVD--LITTGDFMSRFCSNLCLPKQ 118
Query: 225 VRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRN 284
V+ A I +A + L G+SP + AAA+Y+AS EKRTQ+E+ ++A V +VT+R
Sbjct: 119 VQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQ 178
Query: 285 RYK 287
Y+
Sbjct: 179 SYR 181
>pdb|1VOL|A Chain A, Tfiib (Human Core Domain)TBP (A.THALIANA)TATA ELEMENT
Ternary Complex
Length = 204
Score = 123 bits (305), Expect = 3e-27
Identities = 60/176 (34%), Positives = 106/176 (60%), Gaps = 2/176 (1%)
Query: 112 ALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRT 171
A E+ + ++ LP++ + L+R+A + ++GR+ +++ +AC+Y ACR VPRT
Sbjct: 5 AFKEITTMADRINLPRNKVDRTNNLFRQAYEQKSLKGRANDAIASACLYIACRQEGVPRT 64
Query: 172 LDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIE 231
EI ++R+ KKEIGR ++ I + L + + D++++F L L ++V+ A
Sbjct: 65 FKEICAVSRISKKEIGRCFKLILKALETSVD--LITTGDFMSRFCSNLCLPKQVQMAATH 122
Query: 232 ILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYK 287
I +A + L G+SP + AAA+Y+AS EKRTQ+E+ ++A V +VT+R Y+
Sbjct: 123 IARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYR 178
>gi|6325343 transcription factor TFIIB homolog; Sua7p [Saccharomyces
cerevisiae] >gi|135633|sp|P29055|TF2B_YEAST
TRANSCRIPTION INITIATION FACTOR IIB (TFIIB)
(TRANSCRIPTION FACTOR E) >gi|283283|pir||S26707
transcription initiation factor IIB - yeast
(Saccharomyces cerevisiae) >gi|172777|gb|AAA35126.1|
(M81380) Sua7 [Saccharomyces cerevisiae]
>gi|1230692|gb|AAB68135.1| (U51033) Transcription
initiation factor IIB (Swiss Prot. accession number
P29055) [Saccharomyces cerevisiae]
Length = 345
Score = 116 bits (287), Expect = 4e-25
Identities = 89/303 (29%), Positives = 144/303 (47%), Gaps = 26/303 (8%)
Query: 7 CPVCGSTEFIYDPE------RGEIVCARCGYVIEENIVDMGPEWRAF--DASQREKRSRT 58
CP C +Y P+ G++VCA CG V+ + +VD EWR F D + SR
Sbjct: 24 CPECK----VYPPKIVERFSEGDVVCALCGLVLSDKLVDTRSEWRTFSNDDHNGDDPSRV 79
Query: 59 GAPESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDR 118
G + LL LST IG + T + K L K Q + V D + + A +++
Sbjct: 80 GEASNPLLDGNNLSTRIGKGET-TDMRFTK--ELNKAQGK-NVMDKKDNEVQAAFAKITM 135
Query: 119 ITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDI 178
+ +LPK V++ A Y+ + ++G+S+ES++AA + CR +V RT EI +
Sbjct: 136 LCDAAELPKIVKDCAKEAYKLCHDEKTLKGKSMESIMAASILIGCRRAEVARTFKEIQSL 195
Query: 179 ARVEKKEIGRSYRFIARNLNLTPKKLFVK-PTD---------YVNKFADELGLSEKVRRR 228
V+ KE G++ + L + F+K TD Y+ +F LGL +V
Sbjct: 196 IHVKTKEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIPRFCSHLGLPMQVTTS 255
Query: 229 AIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
A ++ + +GKSP + ++Y+ LL T +V + +VTE T+++ YK
Sbjct: 256 AEYTAKKCKEIKEIAGKSPITIAVVSIYLNILLFQIPITAAKVGQTLQVTEGTIKSGYKI 315
Query: 289 LVE 291
L E
Sbjct: 316 LYE 318
>gb|AAB38876.1| (U75276) TFIIB related factor hBRF [Homo sapiens]
Length = 677
Score = 115 bits (286), Expect = 5e-25
Identities = 85/287 (29%), Positives = 138/287 (47%), Gaps = 27/287 (9%)
Query: 5 RVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESI 64
RVC CG T+ D RG+ VC CG V+E+NI+ + F S S G + +
Sbjct: 4 RVCRGCGGTDIELDAARGDAVCTACGSVLEDNIIVSEVQ---FVESSGGGSSAVG--QFV 58
Query: 65 LLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLK 124
L G + +G G + + +SR + R++ + QL+
Sbjct: 59 SLDGAGKTPTLG------GGFHVNLGK----ESRAQTLQNGRRHI-------HHLGNQLQ 101
Query: 125 LPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKK 184
L +H + A ++ AV + L RGR + VIAAC+Y CR P L ++SD+ +V
Sbjct: 102 LNQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVY 161
Query: 185 EIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK---VRRRAIEILEEAYKRGL 241
+G+++ +AR L + + P Y+ +FA L EK V A+ +L+ + +
Sbjct: 162 VLGKTFLLLARELCINAPA--IDPCLYIPRFAHLLEFGEKNHEVSMTALRLLQRMKRDWM 219
Query: 242 TSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
+G+ P+GL AAL +A+ + +RT +EV V +V E T+R R E
Sbjct: 220 HTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTE 266
>gi|4507355 TATA box binding protein (TBP)-associated factor, RNA polymerase
III, GTF3B subunit 2; TATA box binding protein
(TBP)-associated factor, RNA polymerase III, C, 90kD;
general transcription factor IIIB, 90kD [Homo sapiens]
>gi|2136306|pir||I39065 transcription initiation factor
IIIB 90K chain - human >gi|927598|gb|AAC50170.1|
(U28838) TFIIIB 90 kDa subunit [Homo sapiens]
>gi|1581601|prf||2116442A transcription factor IIIB
[Homo sapiens]
Length = 675
Score = 113 bits (279), Expect = 3e-24
Identities = 84/287 (29%), Positives = 136/287 (47%), Gaps = 27/287 (9%)
Query: 5 RVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESI 64
RVC CG T+ D RG+ VC CG V+E+NI+ + F S S G + +
Sbjct: 4 RVCRGCGGTDIELDAARGDAVCTACGSVLEDNIIVSEVQ---FVESSGGGSSAVG--QFV 58
Query: 65 LLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLK 124
L G + +G G + + +SR + R++ + QL+
Sbjct: 59 SLDGAGKTPTLG------GGFHVNLGK----ESRAQTLQDGRRHI-------HHLGNQLQ 101
Query: 125 LPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKK 184
L +H + A ++ AV + L RGR + VIAAC+Y CR P L +SD+ +V
Sbjct: 102 LNQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLVLSDLLQVNVY 161
Query: 185 EIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK---VRRRAIEILEEAYKRGL 241
+G+++ +AR L + + P Y+ +FA L EK V A+ +L+ + +
Sbjct: 162 VLGKTFLLLARELCINAPA--IDPCLYIPRFAHLLEFGEKNHEVSMTALRLLQRMKRDWM 219
Query: 242 TSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
+G+ P+GL AL +A+ + +RT +EV V +V E T+R R E
Sbjct: 220 HTGRGPSGLCGGALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTE 266
>gb|AAF72065.1|AF262374_1 (AF262374) RNA polymerase III transcription factor BRF [Drosophila
melanogaster]
Length = 662
Score = 109 bits (270), Expect = 4e-23
Identities = 78/288 (27%), Positives = 130/288 (45%), Gaps = 27/288 (9%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
C CGS E D RG+ VC CG V+E++++ ++ + + +
Sbjct: 7 CRNCGSNEIEEDNARGDRVCMNCGSVLEDSLIVSEVQF------EEVGHGAAAIGQFVSA 60
Query: 67 HDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLP 126
G +T+ G + G +++ E + A ++ + QL+L
Sbjct: 61 ESSGGATNYGYGKFQVG----------------SGTESREVTIKKAKKDITLLCQQLQLS 104
Query: 127 KHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEI 186
+H + A ++ A+ + L RGR + AACVY CR L +ISD+ ++ E+
Sbjct: 105 QHYADTALNFFKMALGRHLTRGRKSTHIYAACVYMTCRTEGTSHLLIDISDVQQICSYEL 164
Query: 187 GRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK---VRRRAIEILEEAYKRGLTS 243
GR+Y ++ L + L P Y+ +FA+ L L K V A+ I++ K + S
Sbjct: 165 GRTYLKLSHALCINIPSL--DPCLYIMRFANRLQLGAKTHEVSMTALRIVQRMKKDCMHS 222
Query: 244 GKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVE 291
G+ P GL AAL IA+ + RT +V V ++ E T+R R E E
Sbjct: 223 GRRPTGLCGAALLIAARMHDFSRTMLDVIGVVKIHESTLRKRLSEFAE 270
>gb|AAF72066.1|AF262375_1 (AF262375) RNA polymerase III transcription factor BRF [Drosophila
melanogaster]
Length = 662
Score = 109 bits (270), Expect = 4e-23
Identities = 78/288 (27%), Positives = 130/288 (45%), Gaps = 27/288 (9%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
C CGS E D RG+ VC CG V+E++++ ++ + + +
Sbjct: 7 CRNCGSNEIEEDNARGDRVCMNCGSVLEDSLIVSEVQF------EEVGHGAAAIGQFVSA 60
Query: 67 HDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLP 126
G +T+ G + G +++ E + A ++ + QL+L
Sbjct: 61 ESSGGATNYGYGKFQVG----------------SGTESREVTIKKAKKDITLLCQQLQLS 104
Query: 127 KHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEI 186
+H + A ++ A+ + L RGR + AACVY CR L +ISD+ ++ E+
Sbjct: 105 QHYADTALNFFKMALGRHLTRGRKSTHIYAACVYMTCRTEGTSHLLIDISDVQQICSYEL 164
Query: 187 GRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK---VRRRAIEILEEAYKRGLTS 243
GR+Y ++ L + L P Y+ +FA+ L L K V A+ I++ K + S
Sbjct: 165 GRTYLKLSHALCINIPSL--DPCLYIMRFANRLQLGAKTHEVSMTALRIVQRMKKDCMHS 222
Query: 244 GKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVE 291
G+ P GL AAL IA+ + RT +V V ++ E T+R R E E
Sbjct: 223 GRRPTGLCGAALLIAARMHDFSRTMLDVIGVVKIHESTLRKRLSEFAE 270
>pir||T16368 hypothetical protein F45E12.2 - Caenorhabditis elegans
>gi|868262|gb|AAA68790.1| (U29536) similar to
transcription initiation factor IIB [Caenorhabditis
elegans]
Length = 759
Score = 108 bits (267), Expect = 9e-23
Identities = 72/287 (25%), Positives = 131/287 (45%), Gaps = 31/287 (10%)
Query: 5 RVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESI 64
R C CGS+E D RG+ C CG V+EE+IV ++ + + R G
Sbjct: 3 RTCSNCGSSEIDEDAARGDATCTACGTVLEESIV----------VTENQFQERAGGSGHT 52
Query: 65 LLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLK 124
L+ + +S++ + G+ + ++ E A +D + +QL+
Sbjct: 53 LV-GQFVSSERAAANNFNGMGSQ---------------ESREMTYAKGRKVIDELGSQLR 96
Query: 125 LPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKK 184
+ H A Y+ V + L RGR+ SV+A C+Y CRL L + SD+ ++
Sbjct: 97 INSHCMNTAFNFYKMCVSRNLTRGRNRSSVVAVCMYITCRLENTAHLLLDFSDVTQINVF 156
Query: 185 EIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK---VRRRAIEILEEAYKRGL 241
++GR+ +++R+L + P Y+ +FA L +K V A +++ + +
Sbjct: 157 DLGRNLNYLSRSLRINLPS--TDPCLYIMRFACVLDFGDKQKEVVNLATRLVQRMKRDWM 214
Query: 242 TSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
++G+ P G+ AAL IA+ R+ ++ V ++E +R R E
Sbjct: 215 STGRRPTGICGAALLIAARSLNFNRSINDIVRVVHISEGVIRKRLDE 261
>emb|CAB89885.1| (AL354632) transcription factor iiib 70 kd subunit
[Schizosaccharomyces pombe]
Length = 500
Score = 105 bits (259), Expect = 7e-22
Identities = 74/285 (25%), Positives = 128/285 (43%), Gaps = 28/285 (9%)
Query: 7 CPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILL 66
CP CGST F D G C +CG V+E+ DA E + + ++
Sbjct: 11 CPNCGSTTFESDTASGNTYCTQCGVVVEQ------------DAIVSEVTFGEASTGAAVV 58
Query: 67 HDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLP 126
+S D R+ G R + ++ E +A + + LKL
Sbjct: 59 QGSLVSNDQTHARTFGGPYRNQ-----------GSVESRELTIANGRRRISALAIALKLN 107
Query: 127 KHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEI 186
+ E A R + A+ I+GR + V+A+C+Y CR+ K L + SDI ++ ++
Sbjct: 108 ERHIEAAVRYFTLAINNNFIKGRRSQYVVASCLYIVCRISKTSHMLIDFSDILQINVFKL 167
Query: 187 GRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGL---SEKVRRRAIEILEEAYKRGLTS 243
G ++ + R L P + P+ Y+++FA L + +V AI ++ + +
Sbjct: 168 GSTFLKLCRVLR--PNLPLLDPSLYISRFASLLEFGPETHRVANDAIRLVARMNRDWMQI 225
Query: 244 GKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
G+ PAG+ A L IA+ + +R+ REV V +V ++T++ R E
Sbjct: 226 GRRPAGICGACLLIAARMNNFRRSVREVVHVVKVADITIQKRLDE 270
>sp|P46070|TF3B_KLULA TRANSCRIPTION FACTOR IIIB 70 KD SUBUNIT (TFIIIB) (B-RELATED FACTOR)
(BRF1) >gi|619493|emb|CAA87399.1| (Z47203) TFIIB related
subunit of TFIIIB (BRF1) [Kluyveromyces lactis]
Length = 556
Score = 102 bits (253), Expect = 4e-21
Identities = 78/300 (26%), Positives = 134/300 (44%), Gaps = 31/300 (10%)
Query: 5 RVCPVCGSTEFIYDPER--GEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPE 62
R C CGST+F+ D E++C CG V EEN + AF + GA
Sbjct: 10 RKCKNCGSTDFVRDISNTTNELICKVCGLVTEENSI---VSELAFGEASNGAAVIQGA-- 64
Query: 63 SILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQ 122
++ + Q+ L + E L A +L ++
Sbjct: 65 -----------------FVSANQAHPTFMSHSGQNALM---SRETTLNNARRKLKAVSYA 104
Query: 123 LKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVE 182
L +P++V + A + YR A+ ++GR ++VIAAC+Y ACR + L + S +V
Sbjct: 105 LNIPEYVTDAAFQWYRLALSNNFVQGRKSQNVIAACLYIACRKERTHHMLIDFSSRLQVS 164
Query: 183 KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSE---KVRRRAIEILEEAYKR 239
IG ++ +A+ L + K P+ ++ FA++L L + KV R A+++ + +
Sbjct: 165 VYSIGATFLKLAKKLQIV-KLPLADPSLFIQHFAEKLELGDKKIKVIRDAVKLAQTMSRD 223
Query: 240 GLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPV 299
+ G+ PAG+ A L +A + +RT E+ ++ V E T++ R E K+ V
Sbjct: 224 WMYEGRRPAGIAGACLLLACRMNNLRRTHSEIVAISHVAEETLQQRLNEFKNTTSAKLSV 283
>pir||A55483 transcription initiation factor IIIB chain BRF - yeast
(Kluyveromyces marxianus var. lactis)
Length = 556
Score = 102 bits (253), Expect = 4e-21
Identities = 78/300 (26%), Positives = 134/300 (44%), Gaps = 31/300 (10%)
Query: 5 RVCPVCGSTEFIYDPER--GEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPE 62
R C CGST+F+ D E++C CG V EEN + AF + GA
Sbjct: 10 RKCKNCGSTDFVRDISNTTNELICKVCGLVTEENSI---VSELAFGEASNGAAVIQGA-- 64
Query: 63 SILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQ 122
++ + Q+ L + E L A +L ++
Sbjct: 65 -----------------FVSANQAHPTFMSHSGQNALM---SRETTLNNARRKLKAVSYA 104
Query: 123 LKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVE 182
L +P++V + A + YR A+ ++GR ++VIAAC+Y ACR + L + S +V
Sbjct: 105 LNIPEYVTDAAFQWYRLALSNNFVQGRKSQNVIAACLYIACRKERTHHMLIDFSSRLQVS 164
Query: 183 KKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSE---KVRRRAIEILEEAYKR 239
IG ++ +A+ L + K P+ ++ FA++L L + KV R A+++ + +
Sbjct: 165 VYSIGATFLKLAKKLQIV-KLPLADPSLFIQHFAEKLELGDKKIKVIRDAVKLAQTMSRD 223
Query: 240 GLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPV 299
+ G+ PAG+ A L +A + +RT E+ ++ V E T++ R E K+ V
Sbjct: 224 WMYEGRRPAGIAGACLLLACRMNNLRRTHSEIVAISHVAEETLQQRLNEFKNTTSAKLSV 283
>sp|P43072|TF3B_CANAL TRANSCRIPTION FACTOR IIIB 70 KD SUBUNIT (TFIIIB) (B-RELATED FACTOR)
(BRF) >gi|1076945|pir||B55483 transcription initiation
factor IIIB chain BRF - yeast (Candida albicans)
>gi|619452|emb|CAA87398.1| (Z47202) TFIIB related
subunit of TFIIIB (BRF1) [Candida albicans]
Length = 553
Score = 101 bits (248), Expect = 1e-20
Identities = 71/292 (24%), Positives = 135/292 (45%), Gaps = 34/292 (11%)
Query: 3 KQRVCPVCGSTEFIYD--PERGEIVCARCGYVIEEN-IVDMGPEWRAFDASQREKRSRTG 59
KQ+ C CG T+F + G++ C RCG V+EEN IV + + + + G
Sbjct: 6 KQQKCKTCGHTQFDVNRYTAAGDVSCLRCGTVLEENPIVSEVQFGESSSGAAMVQGAMVG 65
Query: 60 APESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRI 119
A D+ +T G R ++ E+ L+ ++ RI
Sbjct: 66 A-------DQARATFAG--------------------GRQNAMESREQTLSNGKRKIKRI 98
Query: 120 TAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIA 179
A LK+P ++ E A +R A+ ++GR +V+A C+Y ACR + L + S
Sbjct: 99 AAALKIPDYIAEAAGEWFRLALTLNFVQGRRSNNVLATCLYVACRKERTHHMLIDFSSRL 158
Query: 180 RVEKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKR 239
++ +G ++ + + L++T L P+ ++ F ++L +K + A + ++ A++
Sbjct: 159 QISVYSLGATFLKMVKALHITSLPL-ADPSLFIQHFVEKLDFKDKATKVAKDAVKLAHRM 217
Query: 240 G---LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
+ G+ PAG+ A + +A+ + +R+ E+ V+ V E T++ R E
Sbjct: 218 AADWIHEGRRPAGIAGACVLLAARMNNFRRSHAEIVAVSHVGEETLQRRLNE 269
>emb|CAB16810.1| (Z99708) transcription initiation factor like protein [Arabidopsis
thaliana] >gi|7270613|emb|CAB80331.1| (AL161589)
transcription initiation factor like protein
[Arabidopsis thaliana]
Length = 503
Score = 100 bits (246), Expect = 3e-20
Identities = 63/205 (30%), Positives = 107/205 (51%), Gaps = 1/205 (0%)
Query: 87 EKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLI 146
E+ L S S NL + +D + + L L + E A +L+R+ +
Sbjct: 109 ERTLELASSTSNSNSSTVVVDNLRAYMQIID-VASILGLDCDISEHAFQLFRDCCSATCL 167
Query: 147 RGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFV 206
R RS+E++ AC+ A R + PRTL EIS A V++KEIG+ + + L L+
Sbjct: 168 RNRSVEALATACLVQAIREAQEPRTLQEISIAANVQQKEIGKYIKILGEALQLSQPINSN 227
Query: 207 KPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKR 266
+ ++ +F L L++ + A I E + + ++P + AAA+Y+A LE +++
Sbjct: 228 SISVHMPRFCTLLQLNKSAQELATHIGEVVINKCFCTRRNPISISAAAIYLACQLEDKRK 287
Query: 267 TQREVAEVARVTEVTVRNRYKELVE 291
TQ E+ ++ +TEVT+R YKEL+E
Sbjct: 288 TQAEICKITGLTEVTLRKVYKELLE 312
>dbj|BAA99940.1| (AP001309) transcription initiation factor IIB (TFIIB)-like
[Arabidopsis thaliana]
Length = 336
Score = 98.3 bits (241), Expect = 1e-19
Identities = 75/290 (25%), Positives = 138/290 (46%), Gaps = 15/290 (5%)
Query: 13 TEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAF---DASQREKRSRTGAPESILLHDK 69
T + D G+ +C+ CG V+E +I++ EWR F D +R GA + L
Sbjct: 13 TIMVVDHSSGDTICSECGLVLEAHIIEYSQEWRTFASDDNHSDRDPNRVGAATNPFLKSG 72
Query: 70 GLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHV 129
L T I + + K +++ +V + E + A E+ R+T L L +
Sbjct: 73 DLVTIIEKPKETASSVLSKDDISTLFRAHNQVKNHEEDLIKQAFEEIQRMTDALDLDIVI 132
Query: 130 EEEAARL---YREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIAR-VEKKE 185
A + Y L RG+ + ++ AA V ACR L++ RTL EI+++A V+KK+
Sbjct: 133 NSRACEIVSKYDGHANTKLRRGKKLNAICAASVSTACRELQLSRTLKEIAEVANGVDKKD 192
Query: 186 IGRSYRFIARNL-----NLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRG 240
I + I R L +++ + + + V +F +L +S++ E +E+A
Sbjct: 193 IRKESLVIKRVLESHQTSVSASQAIINTGELVRRFCSKLDISQREIMAIPEAVEKA--EN 250
Query: 241 LTSGKSPAGLVAAALY-IASLLEGEKRTQREVAEVARVTEVTVRNRYKEL 289
++P ++AA ++ I+ + + ++ RE+ VA V E T++N K++
Sbjct: 251 FDIRRNPKSVLAAIIFMISHISQTNRKPIREIGIVAEVVENTIKNSVKDM 300
>gb|AAG01118.1|AF273333_3 (AF273333) BAC19.3 [Lycopersicon esculentum]
Length = 425
Score = 97.1 bits (238), Expect = 2e-19
Identities = 61/205 (29%), Positives = 107/205 (51%), Gaps = 1/205 (0%)
Query: 87 EKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLI 146
E++ + S S NL L +D + + L+L + + A +L+R+ +
Sbjct: 109 ERVLEMTSTSSSSSSSSVVVENLRAYLQIID-VASILRLDSDISDHAFQLFRDCSSATCL 167
Query: 147 RGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLFV 206
R RS+E++ A + A R + PRTL EIS A + +KEIG+ + + L L+
Sbjct: 168 RNRSVEALATAALVHAIREAQEPRTLQEISVAANLPQKEIGKYIKILGEALQLSQPINSN 227
Query: 207 KPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKR 266
+ ++ +F L L++ + A I E + + ++P + AAA+Y+A LE +++
Sbjct: 228 SISVHMPRFCTLLQLNKSAQELATHIGEVIINKCFCTRRNPISISAAAIYLACQLEDKRK 287
Query: 267 TQREVAEVARVTEVTVRNRYKELVE 291
TQ E+ +V +TEVT+R YKEL+E
Sbjct: 288 TQAEICKVTGLTEVTLRKVYKELLE 312
>gi|11437381 TATA box binding protein (TBP)-associated factor, RNA polymerase
III, GTF3B subunit 2 [Homo sapiens]
Length = 695
Score = 96.7 bits (237), Expect = 3e-19
Identities = 57/170 (33%), Positives = 93/170 (54%), Gaps = 5/170 (2%)
Query: 122 QLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARV 181
QL+L +H + A ++ AV + L RGR + VIAAC+Y CR P L ++SD+ +V
Sbjct: 19 QLQLNQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQV 78
Query: 182 EKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEK---VRRRAIEILEEAYK 238
+G+++ +AR L + + P Y+ +FA L EK V A+ +L+ +
Sbjct: 79 NVYVLGKTFLLLARELCINAPA--IDPCLYIPRFAHLLEFGEKNHEVSMTALRLLQRMKR 136
Query: 239 RGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
+ +G+ P+GL AAL +A+ + +RT +EV V +V E T+R R E
Sbjct: 137 DWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTE 186
>pdb|1PFT| N-Terminal Domain Of Tfiib, Nmr
Length = 50
Score = 96.0 bits (235), Expect = 5e-19
Identities = 40/49 (81%), Positives = 44/49 (89%)
Query: 1 MTKQRVCPVCGSTEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDA 49
+ KQ+VCP C S E IYDPERGEIVCA+CGYVIEENI+DMGPEWRAFDA
Sbjct: 2 VNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDA 50
>gi|6321685 RNA polymerase III transcription factor with homology to TFIIB;
Brf1p [Saccharomyces cerevisiae]
>gi|135646|sp|P29056|TF3B_YEAST TRANSCRIPTION FACTOR
IIIB 70 KD SUBUNIT (TFIIIB) (B-RELATED FACTOR) (BRF)
>gi|322950|pir||A44072 transcription factor IIIB chain
BRF1 - yeast (Saccharomyces cerevisiae)
>gi|172897|gb|AAB04945.1| (M91073) transcription factor
III B component [Saccharomyces cerevisiae]
>gi|172905|gb|AAA35148.1| (L00630) transcription factor
IIIB [Saccharomyces cerevisiae]
>gi|258516|gb|AAB23865.1| RNA polymerase III
transcription factor 4, PCF4, pol III transcription
initiation factor 4=TFIIB homolog [Saccharomyces
cerevisiae, Peptide, 596 aa] >gi|1323446|emb|CAA97275.1|
(Z73031) ORF YGR246c [Saccharomyces cerevisiae]
>gi|1702956|emb|CAA68968.1| (Y07703) BRF1 [Saccharomyces
cerevisiae]
Length = 596
Score = 94.8 bits (232), Expect = 1e-18
Identities = 75/299 (25%), Positives = 135/299 (45%), Gaps = 34/299 (11%)
Query: 6 VCPVCGSTEFIYDPERG--EIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPES 63
VC C TEF D ++VC CG V E+N + + T A +
Sbjct: 3 VCKNCHGTEFERDLSNANNDLVCKACGVVSEDNPIVSEVTF-----------GETSAGAA 51
Query: 64 ILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQL 123
++ +G G + G S L +A N A +L ++ L
Sbjct: 52 VV---QGSFIGAGQSHAAFG-----------GSSALESREATLNN---ARRKLRAVSYAL 94
Query: 124 KLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEK 183
+P+++ + A + Y+ A+ ++GR ++VIA+C+Y ACR K L + S +V
Sbjct: 95 HIPEYITDAAFQWYKLALANNFVQGRRSQNVIASCLYVACRKEKTHHMLIDFSSRLQVSV 154
Query: 184 KEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSE---KVRRRAIEILEEAYKRG 240
IG ++ + + L++T L P+ ++ FA++L L++ KV + A+++ + K
Sbjct: 155 YSIGATFLKMVKKLHITELPL-ADPSLFIQHFAEKLDLADKKIKVVKDAVKLAQRMSKDW 213
Query: 241 LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGIKVPV 299
+ G+ PAG+ A + +A + +RT E+ V+ V E T++ R E K+ V
Sbjct: 214 MFEGRRPAGIAGACILLACRMNNLRRTHTEIVAVSHVAEETLQQRLNEFKNTKAAKLSV 272
>emb|CAC12149.1| (AL445066) transcription initiation factor IIB related protein
[Thermoplasma acidophilum]
Length = 228
Score = 92.1 bits (225), Expect = 7e-18
Identities = 63/225 (28%), Positives = 117/225 (52%), Gaps = 8/225 (3%)
Query: 77 IDRSLTGLMREKMY-----RLRKWQS-RLRVSDAAERNLAFALSELDRITAQLKLPKHVE 130
++R T L R+++ RL++++ R+ + + + NL L R LP++V
Sbjct: 1 MERPETDLDRDELVSSFLGRLKRYKGLRIPENISKDENLNRIAPYLKRSGLMADLPENVI 60
Query: 131 EEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEIGRSY 190
+A +LY E+ +G+ RGR +V+AAC+ A R P TL I+ + + R+
Sbjct: 61 VDALKLYLESSSRGVSRGRGCVAVLAACLLIASRRQANPMTLKYIARSMHTDPSRVWRTE 120
Query: 191 RFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILEEAYKRGLTSGKSPAGL 250
I +N+ TP + +P D+V ++++ L + VR AI ++++ + K+P+ +
Sbjct: 121 NAIVKNMK-TPS-ISARPMDFVERYSELLNVDNDVRSEAIALVQQFSREMRIMRKAPSTI 178
Query: 251 VAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGI 295
+A+Y+AS + QR V + +TEVT+ YKE+ E L +
Sbjct: 179 AISAIYLASERLSKHLVQRYVVKKTGITEVTLIKTYKEMAEYLSM 223
>pir||T01909 transcription initiation factor IIB homolog T12H20.13 - Arabidopsis
thaliana >gi|3600041|gb|AAC35529.1| (AF080119) contains
similarity to transcription factor TFIIB repeat domains
(Pfam: transcript_fac2.hmm, score: 49.16) [Arabidopsis
thaliana] >gi|4539446|emb|CAB40034.1| (AL049523)
putative protein [Arabidopsis thaliana]
>gi|7267766|emb|CAB81169.1| (AL161518) putative protein
[Arabidopsis thaliana]
Length = 182
Score = 83.1 bits (202), Expect = 4e-15
Identities = 52/194 (26%), Positives = 95/194 (48%), Gaps = 20/194 (10%)
Query: 1 MTKQRVCPVCGS-TEFIYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTG 59
M + +C C TE + + + G+ +C CG+VIE NI+D D + ++G
Sbjct: 1 MKEDGICLECKRPTETVVNYKNGDTICIECGHVIENNIID------DLDGASTNPNLKSG 54
Query: 60 APESILLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRI 119
+I+ G S+ + +LR+ Q+ + + E + A +E++R+
Sbjct: 55 HLPTIIFKLSGKSSSLAS-------------KLRRTQNEMIKNKQEEDVIKIAYAEIERM 101
Query: 120 TAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIA 179
T L L + A ++ + +K L G+S+ + AA V ACR + +P+TL EIS +A
Sbjct: 102 TEALGLTFGISNTACKILSKLDKKNLRGGKSLRGLCAASVSRACRQVNIPKTLKEISAVA 161
Query: 180 RVEKKEIGRSYRFI 193
V+ KEI ++ + +
Sbjct: 162 NVDMKEINKAVKLL 175
>gb|AAF14044.1|AC011436_28 (AC011436) putative transcription factor [Arabidopsis thaliana]
Length = 600
Score = 78.4 bits (190), Expect = 9e-14
Identities = 60/233 (25%), Positives = 111/233 (46%), Gaps = 11/233 (4%)
Query: 65 LLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLK 124
+L + ST++ ++ G + +R QS ++ + ER A EL + L
Sbjct: 29 ILENFHFSTEVTFVKNAAGQSQASGNIVRSVQSG--ITSSRERRFRIARDELMNLKDALG 86
Query: 125 LPKHVEEE---AARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARV 181
+ ++ AA+ + AV + +GR E V A+C+Y CR L + L + S RV
Sbjct: 87 IGDERDDVIVIAAKFFEMAVEQNFTKGRRTELVQASCLYLTCRELNIALLLIDFSSYLRV 146
Query: 182 EKKEIGRSYRFIARNLNLTPKKLF---VKPTDYVNKFADEL--GLSEK-VRRRAIEILEE 235
E+G Y + L L + + V P+ ++++F++ L G + K V A +I+
Sbjct: 147 SVYELGSVYLQLCEMLYLVENRNYEKLVDPSIFMDRFSNSLLKGKNNKDVVATARDIIAS 206
Query: 236 AYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
+ + +G+ P+G+ AALY A+L G K ++ ++ + + E T+ R E
Sbjct: 207 MKRDWIQTGRKPSGICGAALYTAALSHGIKCSKTDIVNIVHICEATLTKRLIE 259
>pir||E72670 hypothetical protein APE0787 - Aeropyrum pernix (strain K1)
>gi|5104450|dbj|BAA79765.1| (AP000060) 308aa long
hypothetical protein [Aeropyrum pernix]
Length = 308
Score = 78.4 bits (190), Expect = 9e-14
Identities = 75/304 (24%), Positives = 132/304 (42%), Gaps = 29/304 (9%)
Query: 16 IYDPERGEIVCARCGYVIEENIVDMGPEWRAFDASQREKRSRTGAPESILLHDKGLSTDI 75
I PE GE VC G V+ E+ E+ A S+ T P S HD + +
Sbjct: 14 IVTPE-GEWVCLDTGEVLRESSF---AEYIA--VSRHSSTPETATPVSFKTHDMNIGARM 67
Query: 76 GIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPKHVEEEAAR 135
S +R + + S + S+ AL +L + LK+P V E AA
Sbjct: 68 SDFSSARNPLRRSLRTSKYATSSISRSEVPTIT---ALQKLKEMVEILKIPTSVHETAAL 124
Query: 136 LYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEIGRSYRFIAR 195
+ R + R +E +AA + A + +P + EI+ A VE + + ++ +++
Sbjct: 125 ILRSYLDSVSRRPGRVEQYVAASIAKAVEIHGIPISSGEIAKAAGVEVESVQKAILQMSQ 184
Query: 196 N--LNLTPKKLFVKPT------------DYVNKFADELGLSEKVRRRAIEILEEAYKRGL 241
+ +K K +++N+ LGL +V+R A+E ++ + K G
Sbjct: 185 TDVVKSLVRKSIAKARFRGYSTKMRRIEEFINRLVTNLGLDPEVKRIALEFVKVSLKAGS 244
Query: 242 TS------GKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLGI 295
+ GK A L AAA+Y+A+ L +Q+++A + + E +R Y+ L++ + I
Sbjct: 245 GASIKTFHGKKSAALAAAAVYLAARLLNYDVSQKKIASILELKESNIRKHYRFLIDNVAI 304
Query: 296 KVPV 299
V V
Sbjct: 305 IVYV 308
>pir||T45815 hypothetical protein F28O9.220 - Arabidopsis thaliana
>gi|6735316|emb|CAB68143.1| (AL137080) putative protein
[Arabidopsis thaliana]
Length = 360
Score = 74.1 bits (179), Expect = 2e-12
Identities = 50/193 (25%), Positives = 93/193 (47%), Gaps = 5/193 (2%)
Query: 108 NLAFALSELDRITAQLKLPKHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLK 167
N A ++ ++ I+ LKLP ++ +A +++ V + RG+ + AAC+Y ACR
Sbjct: 156 NKALSIVSIEAISNGLKLPATIKGQANEIFK--VVESYARGKERNVLFAACIYIACRDND 213
Query: 168 VPRTLDEISDIA-RVEKKEIGRSYRFIARNLNLTPK-KLFVKPTDYVNKFADELGLSEKV 225
+ RT+ EIS A + +I + FIA L + + ++ +++ +F L ++
Sbjct: 214 MTRTMREISRFANKASISDISETVGFIAEKLEINKNWYMSIETANFIKRFCSIFRLDKEA 273
Query: 226 RRRAIEILEEAYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNR 285
A+E E +Y ++P + A +Y+ + L EK + + E V E T++
Sbjct: 274 VEAALEAAE-SYDYMTNGRRAPVSVAAGIVYVIARLSYEKHLLKGLIEATGVAENTIKGT 332
Query: 286 YKELVEKLGIKVP 298
Y +L L VP
Sbjct: 333 YGDLYPNLPTIVP 345
>gb|AAD32827.1|AC007659_9 (AC007659) putative transcription factor IIIB 70 KD subunit
(TFIIIB) [Arabidopsis thaliana]
Length = 565
Score = 73.0 bits (176), Expect = 4e-12
Identities = 57/233 (24%), Positives = 109/233 (46%), Gaps = 11/233 (4%)
Query: 65 LLHDKGLSTDIGIDRSLTGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLK 124
+L + ST++ ++ G + L+ QS + S + ER + A EL + L
Sbjct: 29 ILENFNFSTEVTFVKNAAGQSQASGNILKSVQSGM--SSSRERIIRKATDELMNLRDALG 86
Query: 125 LPKHVEEE---AARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEISDIARV 181
+ ++ A+ +R A+ +GRS E V ++C+Y CR K+ L + S RV
Sbjct: 87 IGDDRDDVIVMASNFFRIALDHNFTKGRSKELVFSSCLYLTCRQFKLAVLLIDFSSYLRV 146
Query: 182 EKKEIGRSYRFIARNLNLTPK---KLFVKPTDYVNKFADEL---GLSEKVRRRAIEILEE 235
++G Y + L +T + V P+ ++ +F++ L + K+ A I+
Sbjct: 147 SVYDLGSVYLQLCDMLYITENHNYEKLVDPSIFIPRFSNMLLKGAHNNKLVLTATHIIAS 206
Query: 236 AYKRGLTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
+ + +G+ P+G+ AALY A+L G K ++ ++ + + E T+ R E
Sbjct: 207 MKRDWMQTGRKPSGICGAALYTAALSHGIKCSKTDIVNIVHICEATLTKRLIE 259
>gb|AAD14528.1| (AC006200) putative transcription factor IIIB 70 KD subunit
(TFIIIB) [Arabidopsis thaliana]
Length = 257
Score = 68.7 bits (165), Expect = 8e-11
Identities = 41/150 (27%), Positives = 75/150 (49%), Gaps = 8/150 (5%)
Query: 147 RGRSIESVIAACVYAACRLLKVPRTLDEISDIARVEKKEIGRSYRFIARNLNLTPKKLF- 205
+GR E V ++C+Y ACR +K+ + S RV E+G Y + L L K +
Sbjct: 7 KGRRAELVQSSCLYLACRDMKISLLFIDFSSYLRVSVYELGSVYLQLCEMLYLVQNKNYE 66
Query: 206 --VKPTDYVNKFADEL-----GLSEKVRRRAIEILEEAYKRGLTSGKSPAGLVAAALYIA 258
V P+ ++ +F + L ++ V A I+ + + +G+ P+G+ AA+Y+A
Sbjct: 67 ELVDPSIFIPRFTNSLLKGAHAKAKDVANTAKNIISSMKRDWIQTGRKPSGICGAAIYMA 126
Query: 259 SLLEGEKRTQREVAEVARVTEVTVRNRYKE 288
+L G ++ ++A+V + E T+ R E
Sbjct: 127 ALSHGIMYSRADIAKVVHMCEATITKRLNE 156
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.136 0.380
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99986196
Number of Sequences: 2977
Number of extensions: 3840058
Number of successful extensions: 12011
Number of sequences better than 1.0e-10: 67
Number of HSP's better than 0.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 30
Number of HSP's that attempted gapping in prelim test: 11771
Number of HSP's gapped (non-prelim): 115
length of query: 300
length of database: 189,106,746
effective HSP length: 58
effective length of query: 242
effective length of database: 154,394,500
effective search space: 37363469000
effective search space used: 37363469000
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 165 (68.7 bits)