BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1916 (lplA-like) DE:LIPOATE-PROTEIN LIGASE A RELATED (249 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||B75110 lipoate-protein ligase A related PAB1916 - Pyrococcu... 491 e-138 pir||B71024 probable lipoate protein ligase - Pyrococcus horikos... 433 e-121 dbj|BAB06531.1| (AP001516) lipoate protein ligase [Bacillus halo... 153 2e-36 pir||T46683 probable lipoate protein ligase snoP [imported] - St... 150 1e-35 pir||D69959 conserved hypothetical protein yqhM - Bacillus subti... 150 1e-35 pir||T28693 hypothetical protein - Streptomyces coelicolor >gi|3... 146 2e-34 pir||C70441 lipoate-protein ligase A - Aquifex aeolicus >gi|2983... 136 2e-31 pir||G69830 lipoate-protein ligase homolog yhfJ - Bacillus subti... 129 2e-29 dbj|BAB04402.1| (AP001509) lipoate-protein ligase [Bacillus halo... 129 2e-29 gb|AAK43260.1| Lipoate-protein ligase A (lplA-2) [Sulfolobus so... 122 4e-27 emb|CAC11654.1| (AL445064) lipoate protein ligase related protei... 121 9e-27 pir||C72462 hypothetical protein APE2342 - Aeropyrum pernix (str... 111 8e-24 gb|AAK41323.1| Lipoate protein ligase A, hypothetical (lplA-like... 111 1e-23 sp|P75394|LPLA_MYCPN PROBABLE LIPOATE-PROTEIN LIGASE A >gi|21463... 110 2e-23 gb|AAG18796.1| (AE004984) lipoate protein ligase; Lpl [Halobacte... 110 2e-23 gb|AAK41369.1| Lipoate protein ligase A, hypothetical (lplA-like... 106 3e-22 gb|AAC44341.1| (U62057) lipoate-protein ligase [Mycoplasma capri... 103 2e-21 sp|P47512|LPLA_MYCGE PROBABLE LIPOATE-PROTEIN LIGASE A >gi|13615... 101 7e-21 pir||A70467 hypothetical protein aq_1952 - Aquifex aeolicus >gi|... 99 3e-20 gb|AAK43257.1| Lipoate-protein ligase A (lplA-1) [Sulfolobus sol... 94 1e-18 sp|P54511|YQHM_BACSU HYPOTHETICAL 22.8 KD PROTEIN IN GCVT-SPOIII... 90 2e-17 sp|Q9Y9E6|LIPB_AERPE PROBABLE LIPOATE-PROTEIN LIGASE B (LIPOATE ... 89 4e-17 sp|P32099|LPLA_ECOLI LIPOATE-PROTEIN LIGASE A >gi|625729|pir||A5... 87 3e-16 gb|AAG20904.1| (AE005157) lipoate protein ligase; LipB [Halobact... 78 1e-13 pir||T39701 Lipoate-protein ligase A - fission yeast (Schizosac... 74 1e-12 pir||B70324 hypothetical protein aq_264 - Aquifex aeolicus >gi|2... 69 4e-11 pir||E82204 lipoate-protein ligase A VC1388 [imported] - Vibrio ... 69 6e-11 >pir||B75110 lipoate-protein ligase A related PAB1916 - Pyrococcus abyssi (strain Orsay) >gi|5458106|emb|CAB49595.1| (AJ248285) LIPOATE-PROTEIN LIGASE A RELATED [Pyrococcus abyssi] Length = 249 Score = 491 bits (1251), Expect = e-138 Identities = 249/249 (100%), Positives = 249/249 (100%) Query: 1 MRFIPLIVARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEK 60 MRFIPLIVARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEK Sbjct: 1 MRFIPLIVARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEK 60 Query: 61 ARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKD 120 ARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKD Sbjct: 61 ARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKD 120 Query: 121 LGLNAGFSGLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRD 180 LGLNAGFSGLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRD Sbjct: 121 LGLNAGFSGLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRD 180 Query: 181 KGIRSIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERY 240 KGIRSIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERY Sbjct: 181 KGIRSIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERY 240 Query: 241 GKDEWNFMK 249 GKDEWNFMK Sbjct: 241 GKDEWNFMK 249 >pir||B71024 probable lipoate protein ligase - Pyrococcus horikoshii >gi|3257911|dbj|BAA30594.1| (AP000006) 249aa long hypothetical lipoate protein ligase [Pyrococcus horikoshii] Length = 249 Score = 433 bits (1102), Expect = e-121 Identities = 211/249 (84%), Positives = 235/249 (93%) Query: 1 MRFIPLIVARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEK 60 MRFIPLIVARPE+QMAIDEAILIARSEG+VPDTVRLYVFKPSSVTIGRFQSVR+DV++EK Sbjct: 1 MRFIPLIVARPEVQMAIDEAILIARSEGRVPDTVRLYVFKPSSVTIGRFQSVRYDVNLEK 60 Query: 61 ARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKD 120 A+EL IPVVRRITGGGSVFHDE+GEITYSVVIS+DYHP LKNIQ+SYRFLAGPLVDALKD Sbjct: 61 AKELGIPVVRRITGGGSVFHDEYGEITYSVVISEDYHPMLKNIQDSYRFLAGPLVDALKD 120 Query: 121 LGLNAGFSGLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRD 180 LG+ A FSGLNDIVV GKKISGSAQTRR+GIILQHGT MY+TRLE+L SVLKVSKEKL+D Sbjct: 121 LGIEAEFSGLNDIVVRGKKISGSAQTRRRGIILQHGTLMYATRLEILGSVLKVSKEKLKD 180 Query: 181 KGIRSIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERY 240 KG++SI+ERVTTLEREGIK+ E YE+LRNSFFRAFPL+EGELTEYELEL ++L+EERY Sbjct: 181 KGVKSIFERVTTLEREGIKVGRNEMYEVLRNSFFRAFPLDEGELTEYELELVEKLIEERY 240 Query: 241 GKDEWNFMK 249 G D+WNF K Sbjct: 241 GNDKWNFQK 249 >dbj|BAB06531.1| (AP001516) lipoate protein ligase [Bacillus halodurans] Length = 276 Score = 153 bits (383), Expect = 2e-36 Identities = 88/274 (32%), Positives = 152/274 (55%), Gaps = 28/274 (10%) Query: 2 RFIPLIVARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKA 61 RFI P MA+DEA+L SEG +P T+R Y + P +++IG FQ V+ ++++E Sbjct: 5 RFIDSGDRDPAYNMALDEALLNWHSEGLIPPTIRFYGWNPPTLSIGYFQKVQKEINMEMV 64 Query: 62 RELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDL 121 + + VRR TGG V HD+ E+TYSV++++++ ++ E+YR ++ +++ +DL Sbjct: 65 EKHQLGFVRRPTGGRGVLHDK--ELTYSVIVTEEHPDMPASVTEAYRIISQGILEGFRDL 122 Query: 122 GLNAGFS-----------------------GLNDIVVNGKKISGSAQTRRKGIILQHGTF 158 GL+A F+ ++VV G+K++GSAQTR+KG+ILQHG+ Sbjct: 123 GLDAYFAVPKTEEEKNALKNPRSAVCFDAPSWYELVVEGRKVAGSAQTRQKGVILQHGSI 182 Query: 159 MYSTRLEVLASVLKVSKEKLRDKGIRSIYERVTTL-EREGIKLSMQETYELLRNSFFR-- 215 + + L + E++R++ R+ + + E + LSM+E + ++ F + Sbjct: 183 VLDIDEDKLFDLFNYPSERVRERMQRNFKNKAVPINELRDVPLSMEEVKKAFKDGFEKGL 242 Query: 216 AFPLEEGELTEYELELAQELVEERYGKDEWNFMK 249 + LE LT+ E +++ +ERY DEWNF K Sbjct: 243 SIKLEPYTLTDAEEAEVKQIAKERYETDEWNFRK 276 >pir||T46683 probable lipoate protein ligase snoP [imported] - Streptomyces nogalater >gi|6093260|emb|CAB59005.1| (AJ224512) SnoP [Streptomyces nogalater] Length = 354 Score = 150 bits (376), Expect = 1e-35 Identities = 88/241 (36%), Positives = 137/241 (56%), Gaps = 8/241 (3%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVR 70 P L MA+DE + + G+ P T+R++ + +V IG FQS+R++VD E A + VVR Sbjct: 111 PALHMALDEVLTAEVAAGRRPPTLRVWEWGAPAVIIGSFQSLRNEVDAEGAARHGVEVVR 170 Query: 71 RITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGL 130 RI+GGG++F + ITYS+ + + L + Q+SY +L ++ AL DLG+ A + L Sbjct: 171 RISGGGAMFVEPGNTITYSLSVPESLVQGL-SFQDSYAYLDDWVLGALGDLGIRAWYQPL 229 Query: 131 NDIVVNGKKISGSAQTR------RKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIR 184 NDI + KI+G+AQ R G +L H T Y + + VL++ +EKL DKG + Sbjct: 230 NDIATDQGKIAGAAQKRVVGPGGGPGAVLHHVTMSYDIDADKMVEVLRIGREKLSDKGTK 289 Query: 185 SIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERYGKDE 244 S +RV L R+ L + + + SF + L EG++T+ EL A+EL ++G E Sbjct: 290 SAKKRVDPLRRQ-TGLPREAVIDRMIESFRARYGLAEGKVTDEELARAEELARTKFGSTE 348 Query: 245 W 245 W Sbjct: 349 W 349 >pir||D69959 conserved hypothetical protein yqhM - Bacillus subtilis >gi|2634887|emb|CAB14384.1| (Z99116) similar to hypothetical proteins from B. subtilis [Bacillus subtilis] Length = 278 Score = 150 bits (375), Expect = 1e-35 Identities = 84/274 (30%), Positives = 148/274 (53%), Gaps = 28/274 (10%) Query: 2 RFIPLIVARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKA 61 RFI A P MA+DEA+L SE K+P +R Y + P+++++G FQ+++ +++ E Sbjct: 7 RFIDSGNASPAFNMALDEALLYWHSEKKIPPVIRFYGWNPATLSVGYFQNIKKEINFEAV 66 Query: 62 RELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDL 121 + N+ VRR TGG V HD+ E+TYSV++S+++ + E+YR ++ ++ ++L Sbjct: 67 HKYNLGFVRRPTGGRGVLHDQ--ELTYSVIVSEEHPEMPATVTEAYRVISEGILQGFRNL 124 Query: 122 GLNAGFS-----------------------GLNDIVVNGKKISGSAQTRRKGIILQHGTF 158 GL+A F+ ++VV G+K++GSAQTR+KG+ILQHG+ Sbjct: 125 GLDAYFAIPRTEKEKESLKNPRSSVCFDAPSWYELVVEGRKVAGSAQTRQKGVILQHGSI 184 Query: 159 MYSTRLEVLASVLKVSKEKLRDKGIRSIYERVTTL-EREGIKLSMQETYELLRNSFFRA- 216 + + L + E++R++ R+ + + E +++M E + + F Sbjct: 185 LLDLDEDKLFDLFLYPSERVRERMQRNFKNKAVAINELIEKRVTMDEARKAFKEGFETGL 244 Query: 217 -FPLEEGELTEYELELAQELVEERYGKDEWNFMK 249 LE EL++ EL+ L E +Y DEWN+ + Sbjct: 245 NIHLEPYELSQEELDFVHHLAETKYASDEWNYKR 278 >pir||T28693 hypothetical protein - Streptomyces coelicolor >gi|3127844|emb|CAA18910.1| (AL023496) hypothetical protein [Streptomyces coelicolor A3(2)] Length = 388 Score = 146 bits (365), Expect = 2e-34 Identities = 86/241 (35%), Positives = 138/241 (56%), Gaps = 8/241 (3%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVR 70 P L MA+DE + + G+ P T+R++ + +V IG FQS+R++VD E A I VVR Sbjct: 145 PALHMALDEVLTAEVAAGRRPPTLRVWEWGAPAVIIGSFQSLRNEVDAEGAARHGIEVVR 204 Query: 71 RITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGL 130 RI+GGG++F + ITYS+ + + L + Q+SY +L ++ AL ++G+ A + L Sbjct: 205 RISGGGAMFVEPGNTITYSLSVPEALVQGL-SFQDSYAYLDDWVLGALGEMGIRAWYQPL 263 Query: 131 NDIVVNGKKISGSAQTR------RKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIR 184 NDI + KI+G+AQ R G +L H T Y + + VL++ +EK+ DKG + Sbjct: 264 NDIATDQGKIAGAAQKRVVGPGGGPGAVLHHVTMSYDIDADKMLQVLRIGREKMSDKGTK 323 Query: 185 SIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERYGKDE 244 S +RV L R+ L+ + E + +SF + L G++T+ ELE A+EL ++ E Sbjct: 324 SAKKRVDPLRRQ-TGLAREAVIERMISSFGGRYGLSRGKVTDEELERARELARTKFSSAE 382 Query: 245 W 245 W Sbjct: 383 W 383 >pir||C70441 lipoate-protein ligase A - Aquifex aeolicus >gi|2983965|gb|AAC07512.1| (AE000748) lipoate-protein ligase A [Aquifex aeolicus] Length = 788 Score = 136 bits (339), Expect = 2e-31 Identities = 86/234 (36%), Positives = 130/234 (54%), Gaps = 6/234 (2%) Query: 15 MAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVRRITG 74 MA+D +L +SEGK+P+T+R FKP V IG Q+V +V +E + I V RRITG Sbjct: 78 MALDSVLLELKSEGKIPNTIRFLQFKPECVLIGYHQAVEQEVRLEYVQREGIEVNRRITG 137 Query: 75 GGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGLNDIV 134 GG+++ DE +I + +I+D K+ ++ R + + AL+ LG+NA F NDI Sbjct: 138 GGAIYFDEL-QIGWE-IIADRESFGNKSYEKITRTICETVAKALRKLGVNAQFRPRNDIE 195 Query: 135 VNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYERVTTLE 194 V GKKISG+ L GT + LE + L++ EKL KGI+S +R+T ++ Sbjct: 196 VEGKKISGTGGVFEGNAFLYQGTILMDVNLERMLKSLQIPVEKLTSKGIKSAEDRITWVK 255 Query: 195 RE-GIKLSMQETYELLRNSFFRAFPLEE--GELTEYELELAQELVEERYGKDEW 245 RE G +E + + + +E GELT+ ELEL +E ++ + DEW Sbjct: 256 REIGRLPEKEEVFNAILEALKEELGIEPYWGELTKEELELFEE-KKDYFKSDEW 308 >pir||G69830 lipoate-protein ligase homolog yhfJ - Bacillus subtilis >gi|2226243|emb|CAA74531.1| (Y14083) hypothetical protein [Bacillus subtilis] >gi|2633361|emb|CAB12865.1| (Z99109) similar to lipoate-protein ligase [Bacillus subtilis] Length = 331 Score = 129 bits (322), Expect = 2e-29 Identities = 82/241 (34%), Positives = 133/241 (55%), Gaps = 13/241 (5%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVR 70 P + +AI+E + + + YV +PS + IG+ Q+ +++ + E I VVR Sbjct: 12 PRINLAIEEYCV--KHLDPEQQYLLFYVNQPS-IIIGKNQNTIEEINTKYVEENGIIVVR 68 Query: 71 RITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGL 130 R++GGG+V+HD G + +S + DD + +++ P++ AL LG+ A SG Sbjct: 69 RLSGGGAVYHD-LGNLNFSFITKDD-----GDSFHNFKKFTEPVIQALHQLGVEAELSGR 122 Query: 131 NDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYERV 190 NDIVV+G+KISG+AQ KG I HGT M+ + ++ + S LKV K+K+ KGI+SI RV Sbjct: 123 NDIVVDGRKISGNAQFATKGRIFSHGTLMFDSAIDHVVSALKVKKDKIESKGIKSIRSRV 182 Query: 191 TTL-EREGIKLSMQETYELLRNSFFRAFP---LEEGELTEYELELAQELVEERYGKDEWN 246 + E K++ +E L F + E +LTE + E ++ +ERY +WN Sbjct: 183 ANISEFLDDKMTTEEFRSHLLRHIFNTNDVGNVPEYKLTEKDWETIHQISKERYQNWDWN 242 Query: 247 F 247 + Sbjct: 243 Y 243 >dbj|BAB04402.1| (AP001509) lipoate-protein ligase [Bacillus halodurans] Length = 330 Score = 129 bits (322), Expect = 2e-29 Identities = 84/245 (34%), Positives = 132/245 (53%), Gaps = 12/245 (4%) Query: 8 VARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIP 67 + P + +AI+E L + +T L+ S+ IGR Q+ +++ E I Sbjct: 9 ITDPRINLAIEEYAL---KNLDINETYLLFYINEPSIIIGRNQNTIEEINTEYVESNGIH 65 Query: 68 VVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGF 127 VVRR++GGG+V+HD G + +S + DD S N Q+ P++ AL LG+ A Sbjct: 66 VVRRLSGGGAVYHDH-GNLNFSFITKDDGE-SFSNFQK----FTDPVIKALAKLGVTAEL 119 Query: 128 SGLNDIVVN-GKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSI 186 G NDI+ + G+KISG+AQ KG + HGT ++ + ++ + S L VSK+K+ KGI+SI Sbjct: 120 KGRNDIIASDGRKISGNAQFSTKGRMFSHGTLLFDSEIDHVVSALNVSKDKIESKGIKSI 179 Query: 187 YERVTTL-EREGIKLSMQETYELLRNSFFRA-FPLEEGELTEYELELAQELVEERYGKDE 244 RV + E K+S+ + LL S F ++E +LT + EL +ERY + Sbjct: 180 RSRVANISEFLTEKISIDQFRSLLLESIFDGQANIQEYKLTADDWAEIHELSKERYQNWD 239 Query: 245 WNFMK 249 WN+ K Sbjct: 240 WNYGK 244 >gb|AAK43260.1| Lipoate-protein ligase A (lplA-2) [Sulfolobus solfataricus] Length = 264 Score = 122 bits (303), Expect = 4e-27 Identities = 85/272 (31%), Positives = 145/272 (53%), Gaps = 35/272 (12%) Query: 1 MRFIPLIVARPE---LQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVD 57 M+ + +IV P MAIDEAI++ R+ DT+RLY++ PS V++GR Q+ + Sbjct: 1 MKMLRIIVDGPRDPYYNMAIDEAIMLTRNSVNY-DTLRLYMWSPSGVSLGRGQNA-NAAY 58 Query: 58 IEKARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDA 117 ++ +EL +VRR TGGG++ H E EITYSVV+S + ++ ES +A ++ Sbjct: 59 LDNIKELGFKLVRRPTGGGALLHPEDNEITYSVVLSASNTIAKLSVDESAVEIAKGILYT 118 Query: 118 LKDLGLNAGFSGL---------------NDIVVNGKKISGSAQTRRKGIILQHGTFMYST 162 L+ LG N GL +D++V G+KISGSAQ R +LQHGT + Sbjct: 119 LQILGENVDIRGLGDMKKHDLCYLRSGSSDVIVAGRKISGSAQVRDDKALLQHGTLLLRF 178 Query: 163 RLEVLASVLK---VSKEKLRDKGIRSIYERVTTLEREGIKLSMQETYELLRNSFFRA--- 216 E+ V++ V++E L+ + + E +++ + + ++ + F +A Sbjct: 179 EPEIWLKVIRAPGVTEEFLKSRIVGLF---------EFMEVEIDQIFDAMIKGFSKALNE 229 Query: 217 FPLEEGELTEYELELAQELVEERYGKDEWNFM 248 + G LT E++L+ +L +E+Y ++WN + Sbjct: 230 IDVFMGSLTPKEIKLSNKLYKEKYSNEKWNLL 261 >emb|CAC11654.1| (AL445064) lipoate protein ligase related protein [Thermoplasma acidophilum] Length = 262 Score = 121 bits (300), Expect = 9e-27 Identities = 87/250 (34%), Positives = 132/250 (52%), Gaps = 28/250 (11%) Query: 13 LQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVRRI 72 + +A DEAI + G P + + SV IG FQ +VD++ ++ I + RR Sbjct: 16 MSLAYDEAIYRSFQYGDKP--ILRFYRHDRSVIIGYFQVAEEEVDLDYMKKNGIMLARRY 73 Query: 73 TGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGLND 132 TGGG+V+HD G++ +SVV S D +I +R + +V++L+ LGL+A LND Sbjct: 74 TGGGAVYHD-LGDLNFSVVRSSDD----MDITSMFRTMNEAVVNSLRILGLDARPGELND 128 Query: 133 ----------IVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKG 182 I+ KKI G+A RKG L H + T L++L++VLKV EK RDK Sbjct: 129 VSIPVNKKTDIMAGEKKIMGAAGAMRKGAKLWHAAMLVHTDLDMLSAVLKVPDEKFRDKI 188 Query: 183 IRSIYERVTTLEREGIKLSMQETYELLRNSFFRAF------PLEEGELTEYELELAQELV 236 +S ERV + + + +S+ E +RN+ R F E +TE E LA+EL Sbjct: 189 AKSTRERVANV-TDFVDVSIDE----VRNALIRGFSETLHIDFREDTITEKEESLARELF 243 Query: 237 EERYGKDEWN 246 +++Y +EWN Sbjct: 244 DKKYSTEEWN 253 >pir||C72462 hypothetical protein APE2342 - Aeropyrum pernix (strain K1) >gi|5106044|dbj|BAA81355.1| (AP000064) 260aa long hypothetical protein [Aeropyrum pernix] Length = 260 Score = 111 bits (275), Expect = 8e-24 Identities = 89/257 (34%), Positives = 131/257 (50%), Gaps = 32/257 (12%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVR 70 P + MAIDEAIL G P T+R+Y++ P +T+GR QSV DV++ +A + +VR Sbjct: 9 PRMNMAIDEAILRV---GAAP-TLRIYMWLPGGLTLGRRQSVE-DVNLAEAEKRGYVLVR 63 Query: 71 RITGGGSVFHDEFGEITYSVVISDDYHPSLK-NIQESYRFLAGPLVDALKDLGLN---AG 126 R TGG ++ H EITYS+V+ D HP ++ ES +AG + AL++LG+ G Sbjct: 64 RPTGGAALLHPWEREITYSLVL-DSRHPYYSLDVAESAATIAGGVALALEELGIATRVGG 122 Query: 127 FS--------------GLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLK 172 ++ G +D+VV G+K+SGSAQ R G +LQHGT + E S++ Sbjct: 123 YTPPPGVIGGLCYVRPGSSDVVVAGRKVSGSAQRRIGGRLLQHGTLLLRFDPEEWLSIIP 182 Query: 173 VSKEKLRDKGIRSIYERVTTLE-REGIKLSMQETYELLRNSFFRAFPL--EEGELTEYEL 229 V + G + R+ +E G S E + L F +A L E L E+ Sbjct: 183 V-----KGAGPSDLASRIAGIEDLAGRSYSFSEVADALVKGFSKALGLEPEPSGLEGREV 237 Query: 230 ELAQELVEERYGKDEWN 246 E A L ++Y WN Sbjct: 238 EEALRLYRKKYSSPRWN 254 >gb|AAK41323.1| Lipoate protein ligase A, hypothetical (lplA-like1) [Sulfolobus solfataricus] Length = 279 Score = 111 bits (274), Expect = 1e-23 Identities = 76/221 (34%), Positives = 120/221 (53%), Gaps = 8/221 (3%) Query: 32 DTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVRRITGGGSVFHDEFGEITYSVV 91 +T+ ++ K V +G Q V +VD+E +++ IPVVRR GGG+V GE Y +V Sbjct: 33 NTLLVFSVKEPFVNVGIHQEVWLEVDLEFTKKMKIPVVRRDLGGGTVVITP-GEHDYFIV 91 Query: 92 ISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGLNDIVVNGKKISGSAQTRRKGI 151 + + P +N E Y+ P+V+ L+ GL A DIVVNGKKISG+ Sbjct: 92 VRQEDAP--RNPSELYKKFLTPIVNVLRSYGLKADLRD-QDIVVNGKKISGNGAMTHGNS 148 Query: 152 ILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYERVTTLERE-GIKLSMQETYELLR 210 I+ G + S +++++ ++V EK RDK + + E +T+LERE G +E + L+ Sbjct: 149 IVIAGNILLSLDIDLISKCIRVPTEKFRDKMAKDMSEWLTSLERELGYIPPKEEINKKLK 208 Query: 211 NSFFR--AFPLEEGELTEYELELAQELVEERYGKDEWNFMK 249 +F + E+ LT E+EL ++L E+ K+EW F K Sbjct: 209 EAFEKELGIKFEDSTLTPEEIELWEKLASEK-AKEEWIFYK 248 >sp|P75394|LPLA_MYCPN PROBABLE LIPOATE-PROTEIN LIGASE A >gi|2146329|pir||S73775 lipoate protein ligase lplA - Mycoplasma pneumoniae (strain ATCC 29342) >gi|1674137|gb|AAB96097.1| (AE000044) lipoate protein ligase [Mycoplasma pneumoniae] Length = 339 Score = 110 bits (272), Expect = 2e-23 Identities = 69/241 (28%), Positives = 122/241 (49%), Gaps = 10/241 (4%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVF--KPSSVTIGRFQSVRHDVDIEKARELNIPV 68 P A++E +L +E K + +++ F +++ +GR Q+ +V++ + + + + Sbjct: 13 PYFNAALEEWLL---TEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNL 69 Query: 69 VRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFS 128 RR +GGG+VFHD G I +S+++ + K ++ +Y +V L +G+ A F Sbjct: 70 FRRFSGGGAVFHD-MGNICFSIILPK----AKKEMENAYEETTRNVVKFLNSVGVPAQFH 124 Query: 129 GLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYE 188 G ND+ + GKK SG A+ K +L HGT ++ T LA L V K K+ KGI S+ + Sbjct: 125 GRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQK 184 Query: 189 RVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERYGKDEWNFM 248 RV ++ LS E + F E L E +++ ++ +E + +WNF Sbjct: 185 RVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWDWNFG 244 Query: 249 K 249 K Sbjct: 245 K 245 >gb|AAG18796.1| (AE004984) lipoate protein ligase; Lpl [Halobacterium sp. NRC-1] Length = 272 Score = 110 bits (272), Expect = 2e-23 Identities = 77/253 (30%), Positives = 123/253 (48%), Gaps = 30/253 (11%) Query: 13 LQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVRRI 72 +QMA+DE ++G P TVR+Y + P ++++G ++ VD A + I V RR Sbjct: 20 MQMALDEIAAQTAADGG-PRTVRVYTWTPDTLSMG-YRQATDSVDWAAAADAGIDVTRRQ 77 Query: 73 TGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSG--- 129 TGGG ++HD +I+YS+V D P ++ ++Y L P+ DA LG++A F+ Sbjct: 78 TGGGGIYHDAHADISYSIVAPADELPG--DLMDTYELLCTPVFDAFDRLGIDARFTDTEQ 135 Query: 130 ---------------LNDIVVN--GKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLK 172 +DIV G+KISG+AQ RR ++QHG+ ++ E SV Sbjct: 136 PALHQPACYLRALNPAHDIVAGDPGRKISGNAQYRRADAVIQHGSLSFALDAERHLSVFS 195 Query: 173 VSKEKLRDKGIRSIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELA 232 D + E VTT+E + + + + + +EGE T+ EL A Sbjct: 196 DP-----DTTPAAFREHVTTIEAQA-GVDRADAVTAVERALGEWADADEGEWTDAELARA 249 Query: 233 QELVEERYGKDEW 245 ++L E+Y D W Sbjct: 250 RDLAAEKYAADAW 262 >gb|AAK41369.1| Lipoate protein ligase A, hypothetical (lplA-like2) [Sulfolobus solfataricus] Length = 344 Score = 106 bits (261), Expect = 3e-22 Identities = 73/239 (30%), Positives = 126/239 (52%), Gaps = 14/239 (5%) Query: 15 MAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVRRITG 74 +A +EA+L++ + G P +R +F P +V +G Q+V +V+IE+ ++ + RR TG Sbjct: 17 LAGEEALLLSVANGSQP-ILRFVIFDPPAVLVGYHQAVEQEVNIEEVKKRGWEIGRRPTG 75 Query: 75 GGSVFHDEFGEITYSVVISDD---YHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGLN 131 GG++ + ++ + + ++D Y P + + + A ++ AL LG+ A F N Sbjct: 76 GGTIIMGPW-QLGWEIYANNDLLGYTP-----ENAIKIGAEGVIRALDKLGIKASFRPKN 129 Query: 132 DIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYERVT 191 D+ VNG+KISG I GT + ++ + SVL++S EKL+DK R +R+T Sbjct: 130 DVEVNGRKISGIGAFSEGNYIAVTGTILLDFDVDAMISVLRLSSEKLKDKLARDFKDRLT 189 Query: 192 TLERE-GIKLSMQETYELLRNSFFR--AFPLEEGELTEYELELAQELVEERYGKDEWNF 247 + +E L M+E ++ R+SF L + E+E + QEL +Y EW F Sbjct: 190 WVNKELKRSLDMEELIKISRDSFAEVLGIKLSDDYYNEFEKKTIQELA-LKYSSPEWIF 247 >gb|AAC44341.1| (U62057) lipoate-protein ligase [Mycoplasma capricolum] Length = 334 Score = 103 bits (255), Expect = 2e-21 Identities = 67/248 (27%), Positives = 127/248 (51%), Gaps = 24/248 (9%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVR 70 P + +AI+E + K + + + +++ +GR Q+ ++++E A++ N+ +V+ Sbjct: 12 PAMNLAIEEYLTY---HYKAKEPIVFFWQNANTIVVGRNQNAFAEINLEAAQKDNVKIVK 68 Query: 71 RITGGGSVFHDEFGEITYSVVISD-----DYHPSLKNIQESYRFLAGPLVDALKDLGLNA 125 R TGGG+V+ D G + YS+++ + DY +L+ P++ L +NA Sbjct: 69 RNTGGGTVYQD-LGNVCYSLIVDNSTDDVDYQKALQ-----------PIITYLNQKNINA 116 Query: 126 GFSGLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRS 185 FSG ND+V++G K+SG+AQ + L HGT ++ L + L V EKL+ + IRS Sbjct: 117 MFSGRNDMVIDGYKVSGNAQLKTNEKTLVHGTLLFDVDLSKMPKYLVVDPEKLKHQQIRS 176 Query: 186 IYERVTTLER----EGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERYG 241 RV ++ I + + + +S+ + ++ ELT+ E + Q E ++ Sbjct: 177 KPARVRNIKEFFKDINIDIDLSTFINDVVSSYVKNEKIKWIELTDQEKQYIQSRKETKFD 236 Query: 242 KDEWNFMK 249 + +W F K Sbjct: 237 QWDWTFGK 244 >sp|P47512|LPLA_MYCGE PROBABLE LIPOATE-PROTEIN LIGASE A >gi|1361583|pir||H64229 hypothetical protein homolog MG270 - Mycoplasma genitalium >gi|1045964|gb|AAC71492.1| (U39706) lipoate protein ligase (lplA) [Mycoplasma genitalium] Length = 336 Score = 101 bits (250), Expect = 7e-21 Identities = 70/244 (28%), Positives = 122/244 (49%), Gaps = 10/244 (4%) Query: 8 VARPELQMAIDEAILIARSEGKVPDTVRLYVF--KPSSVTIGRFQSVRHDVDIEKARELN 65 V P A++E +L +E + + V++ F +++ +GR Q+ +V++++ Sbjct: 10 VFNPYFNAALEEWLL---TEFRKNELVKVIYFWQNANTIVVGRNQNTYAEVNLKELESDK 66 Query: 66 IPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNA 125 + + RR +GGG+VFHD G I +S+++ + K ++ +Y +V L L + A Sbjct: 67 VNLFRRFSGGGAVFHD-LGNICFSIILPR----TGKVMENAYEQTTRNVVKFLNSLNVPA 121 Query: 126 GFSGLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRS 185 F G ND+ +N KK SG A+ K +L HGT ++ T LA L V K K+ KG+ S Sbjct: 122 VFHGRNDLEINNKKFSGLAEYIAKDRLLVHGTLLFDTDFSKLAKYLNVDKTKIASKGVDS 181 Query: 186 IYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVEERYGKDEW 245 + +RV ++ + + E + N F E LT+ L ++ +E + EW Sbjct: 182 VAKRVVNVKEYLPNWTTAKFLEEMINFFTVTEKAETIVLTKDALAKVEKRAKEHFQSWEW 241 Query: 246 NFMK 249 NF K Sbjct: 242 NFGK 245 >pir||A70467 hypothetical protein aq_1952 - Aquifex aeolicus >gi|2984208|gb|AAC07738.1| (AE000765) putative protein [Aquifex aeolicus] Length = 345 Score = 99.5 bits (244), Expect = 3e-20 Identities = 62/198 (31%), Positives = 108/198 (54%), Gaps = 7/198 (3%) Query: 45 TIGRFQSVRHDVDIEKARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQ 104 ++G F + +D+EK ++L IPV+RR GGG+V E ++ Y +++ P + Sbjct: 40 SVGYFDKTQEILDLEKCKKLGIPVIRREVGGGAVLLHE-NQVFYQLILKKSRAPF--KVI 96 Query: 105 ESYRFLAGPLVDALKDLGLNAGFSGLNDIVV--NGKKISGSAQTRRKGIILQHGTFMYST 162 ++Y+ + P+++ + LG+ + +ND+VV N KKISG + + G + Sbjct: 97 DAYKKFSQPVINVYRRLGVEVEYRPINDLVVKRNKKKISGQGAADIEDSFVFVGGILLKF 156 Query: 163 RLEVLASVLKVSKEKLRDKGIRSIYERVTTLEREGIKL-SMQETYELLRNSFFRAFPLE- 220 +++A + ++ +EK RDK +S+ E VT +ERE +L S +E E L F + P E Sbjct: 157 NTKLMAEIFRIPEEKFRDKVHKSLEENVTWVERETGRLPSYEEVEEFLVEEFSKVIPFEG 216 Query: 221 EGELTEYELELAQELVEE 238 E + E +ELA +L EE Sbjct: 217 EEPVPEEAIELADKLKEE 234 >gb|AAK43257.1| Lipoate-protein ligase A (lplA-1) [Sulfolobus solfataricus] Length = 247 Score = 94.4 bits (231), Expect = 1e-18 Identities = 75/248 (30%), Positives = 122/248 (48%), Gaps = 23/248 (9%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVR 70 P L +A+DEA+L+ K +R++ SV +G S+ +V++E ++ ++ + R Sbjct: 14 PYLNVAMDEALLL---HSKYVPILRIWR-NEKSVVLGILSSISDEVNLEMIKKYDVKLAR 69 Query: 71 RITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGL 130 RI+GGGSVFHD G I Y+ + S I Y +L + A+++L +N Sbjct: 70 RISGGGSVFHD-MGNINYTFITSGQ-----GGIDYLYGYLLRGTISAIENL-VNDRVEVY 122 Query: 131 N--DIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYE 188 N DI G KISG+A + L HGT + S+ L++L VL + + R K I I Sbjct: 123 NETDIAFKGYKISGNAGYINEDKYLLHGTLLVSSNLDILHKVLIIPPKNYRKKDINMIKY 182 Query: 189 RVTTLEREGIKLSMQETYELLRNSFFRAF-------PLEEGELTEYELELAQELVEERYG 241 RV+ L + TY+ +F R F ++++ EL+LA L E+Y Sbjct: 183 RVSNLSN---LVKGDITYDEAIEAFIRGFSSLLRCDDYFFDDISDEELKLAMRLANEKYS 239 Query: 242 KDEWNFMK 249 K E+ + + Sbjct: 240 KKEFIYKR 247 >sp|P54511|YQHM_BACSU HYPOTHETICAL 22.8 KD PROTEIN IN GCVT-SPOIIIAA INTERGENIC REGION >gi|1303894|dbj|BAA12550.1| (D84432) YqhM [Bacillus subtilis] Length = 196 Score = 90.5 bits (221), Expect = 2e-17 Identities = 54/198 (27%), Positives = 101/198 (50%), Gaps = 28/198 (14%) Query: 78 VFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFS--------- 128 + HD+ E+TYSV++S+++ + E+YR ++ ++ ++LGL+A F+ Sbjct: 1 MLHDQ--ELTYSVIVSEEHPEMPATVTEAYRVISEGILQGFRNLGLDAYFAIPRTEKEKE 58 Query: 129 --------------GLNDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVS 174 ++VV G+K++GSAQTR+KG+ILQHG+ + + L + Sbjct: 59 SLKNPRSSVCFDAPSWYELVVEGRKVAGSAQTRQKGVILQHGSILLDLDEDKLFDLFLYP 118 Query: 175 KEKLRDKGIRSIYERVTTL-EREGIKLSMQETYELLRNSFFRA--FPLEEGELTEYELEL 231 E++R++ R+ + + E +++M E + + F LE EL++ EL+ Sbjct: 119 SERVRERMQRNFKNKAVAINELIEKRVTMDEARKAFKEGFETGLNIHLEPYELSQEELDF 178 Query: 232 AQELVEERYGKDEWNFMK 249 L E +Y DEWN+ + Sbjct: 179 VHHLAETKYASDEWNYKR 196 >sp|Q9Y9E6|LIPB_AERPE PROBABLE LIPOATE-PROTEIN LIGASE B (LIPOATE BIOSYNTHESIS PROTEIN B) >gi|7521166|pir||B72462 probable lipoate-protein ligase A APE2341 - Aeropyrum pernix (strain K1) >gi|5106043|dbj|BAA81354.1| (AP000064) 264aa long hypothetical lipoate-protein ligase A [Aeropyrum pernix] Length = 264 Score = 89.3 bits (218), Expect = 4e-17 Identities = 67/236 (28%), Positives = 113/236 (47%), Gaps = 12/236 (5%) Query: 11 PELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVR 70 P +A++EA+L +E + RL+V P S+ +G V +V+IE+AR +PVVR Sbjct: 10 PHFNIALEEALLEESAEHGIA-IARLWV-NPDSIIVGYTSDVGREVNIEQARAEGVPVVR 67 Query: 71 RITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGL 130 RI+GGG+VFHD G + SV Y P + E+Y + ++ L LG+ Sbjct: 68 RISGGGAVFHD-LGNMNVSV-----YIPRRLGVDEAYALVTSIILKTLHRLGIEGRVENG 121 Query: 131 NDIVVNGKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYERV 190 ND+ V K+SGSA R L H T + +T + ++ ++ + + Sbjct: 122 NDVAVGPWKVSGSAAAIRARATLAHATLLLTTDPSRIRRLVIPQLHRVERGEVTPVKYNP 181 Query: 191 TTLER-EGIKLSMQETYELLRNSFFRAFPLEEG---ELTEYELELAQELVEERYGK 242 +LER G ++ + + LL S A E ++ + + A+EL + +Y + Sbjct: 182 NSLERITGERMEVWQAARLLEESVKHALGEPENVGRDILQEAVIRARELCKTKYSQ 237 >sp|P32099|LPLA_ECOLI LIPOATE-PROTEIN LIGASE A >gi|625729|pir||A54035 lipoate--protein ligase (EC 6.3.4.-) A [validated] - Escherichia coli >gi|504496|gb|AAA21740.1| (L27665) lipoate-protein ligase A [Escherichia coli] >gi|537226|gb|AAA97282.1| (U14003) yjjF gene product [Escherichia coli] >gi|1790846|gb|AAC77339.1| (AE000508) lipoate-protein ligase A [Escherichia coli K12] Length = 338 Score = 86.6 bits (211), Expect = 3e-16 Identities = 75/251 (29%), Positives = 121/251 (47%), Gaps = 32/251 (12%) Query: 11 PELQMAIDEAILIARSEGKVPDTVR-LYVFKPS-SVTIGRFQSVRHDVDIEKARELNIPV 68 P +A++E I ++P T R L++++ + +V IGR Q+ + + + E N+ + Sbjct: 14 PWFNLAVEECIF-----RQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRL 68 Query: 69 VRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFS 128 RR +GGG+VFHD G ++ + + K I S +++AL LG++A S Sbjct: 69 ARRSSGGGAVFHD-LGNTCFTFMAGKPEYD--KTISTSI------VLNALNALGVSAEAS 119 Query: 129 GLNDIVVN----GKKISGSAQTRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIR 184 G ND+VV +K+SGSA K HGT + + L LA+ L K+KL KGI Sbjct: 120 GRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKGIT 179 Query: 185 SIYERVTTLEREGIKLSMQETYELLRNSFFRAFPLEEGELTEYELELAQELVE------- 237 S+ RVT L ++ ++ E + +FF + GE E E+ + + Sbjct: 180 SVRSRVTNLTELLPGITHEQVCEAITEAFFAHY----GERVEAEIISPNKTPDLPNFAET 235 Query: 238 -ERYGKDEWNF 247 R EWNF Sbjct: 236 FARQSSWEWNF 246 >gb|AAG20904.1| (AE005157) lipoate protein ligase; LipB [Halobacterium sp. NRC-1] Length = 169 Score = 77.6 bits (188), Expect = 1e-13 Identities = 48/160 (30%), Positives = 80/160 (50%), Gaps = 3/160 (1%) Query: 86 ITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGLNDIVVNGKKISGSAQ 145 +TYS+ + + P ++ +SY +L +DAL+ +G++A + LNDIV K+ G+AQ Sbjct: 9 LTYSLYLPPEDVPD--DVADSYVYLDQWAIDALQAVGIDASYEPLNDIVHADGKLGGAAQ 66 Query: 146 TRRKGIILQHGTFMYSTRLEVLASVLKVSKEKLRDKGIRSIYERVTTLEREGIKLSMQET 205 R G +L H T Y +E + VL++ +EKL DK + S +RV + + S Sbjct: 67 LRTDGAVLHHTTMSYDLDIERMLRVLRIGEEKLSDKAVASAEKRVAVM-TDHTDASRATI 125 Query: 206 YELLRNSFFRAFPLEEGELTEYELELAQELVEERYGKDEW 245 + L + F G L+ E A+ L E++ W Sbjct: 126 VDALVAAIGDTFETRRGSLSAATREAARTLAAEQFDTAAW 165 >pir||T39701 Lipoate-protein ligase A - fission yeast (Schizosaccharomyces pombe) >gi|2257523|dbj|BAA21417.1| (AB004537) LIPOATE-PROTEIN LIGASE A [Schizosaccharomyces pombe] >gi|5679728|emb|CAB51768.1| (AL109652) Lipoate-protein ligase A [Schizosaccharomyces pombe] Length = 363 Score = 74.1 bits (179), Expect = 1e-12 Identities = 54/169 (31%), Positives = 91/169 (52%), Gaps = 15/169 (8%) Query: 36 LYVFKPSSVTIGRFQSVRHDVDIEKARELNIPVVRRITGGGSVFHDEFGEITYSVVISDD 95 LY PS V IGR Q+ + +++ R+ + ++RR +GGG+VFHD FG + YSV+++ + Sbjct: 57 LYTNSPS-VIIGRNQNPWVEANVKLCRDNFVNIIRRKSGGGTVFHD-FGNLNYSVLMNRE 114 Query: 96 YHPSLKNIQESYRFLAGPLVDALKDLGLNAGFSGLNDIVV--NGKKISGSAQTRRKGIIL 153 +N A ++ AL++LG++A + +DIV+ + +KISGSA + Sbjct: 115 EFSHTEN--------ASIMIQALRNLGVHARLNQRHDIVLAQSQRKISGSAYKISRNRCY 166 Query: 154 QHGTFMYSTRLEVLASVLKVSKEKLRDKGI---RSIYERVTTLEREGIK 199 HGT + ++ LE + L+ + KG+ RS L+ E IK Sbjct: 167 HHGTMLLNSDLEGVREYLRSPSTGILSKGVSSTRSPVSNTKLLKAEFIK 215 >pir||B70324 hypothetical protein aq_264 - Aquifex aeolicus >gi|2982970|gb|AAC06584.1| (AE000681) putative protein [Aquifex aeolicus] Length = 217 Score = 69.5 bits (167), Expect = 4e-11 Identities = 62/230 (26%), Positives = 99/230 (42%), Gaps = 43/230 (18%) Query: 1 MRFIPLIVARPELQMAIDEAILIARSEGKVPDTVRLYVFKPSSVTIGRFQSVRHDVDIEK 60 MRF+ + PE M IDE + I G++ RLY +K V++GR Q + Sbjct: 1 MRFLGFGIFSPEENMKIDEELQIKLENGEIEPCFRLYKWKGVCVSLGRNQ---------E 51 Query: 61 ARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKNIQESYRFLAGPLVDALKD 120 +E + VVRR TGGG++ H ++++ +V DY +N YR ++ + K+ Sbjct: 52 EKEFPVKVVRRPTGGGALLHG--WDLSFCIV---DYKNG-RNFMRFYREVSKMFYNIFKE 105 Query: 121 LGLNAGFSGLNDIVV-----------------NGKKISGSAQTRRKGIILQHGTFMYSTR 163 G+N F + GKK+ A K L HG+ Sbjct: 106 FGVNLKFERNKSYSLQTYYCYFFPTFGELKTEEGKKVVAIAMRELKNTFLLHGSVYVDFD 165 Query: 164 LEVLASVLKVSKEKLRDKGIRSIYERVTTLEREGIKLSMQETYELLRNSF 213 + +L V +E+LR +RVTTL+ GI +E ++ L S+ Sbjct: 166 YNYASKILNVKEEELR--------KRVTTLKELGIS---EEDFKTLLCSY 204 >pir||E82204 lipoate-protein ligase A VC1388 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9655884|gb|AAF94546.1| (AE004218) lipoate-protein ligase A [Vibrio cholerae] Length = 338 Score = 68.7 bits (165), Expect = 6e-11 Identities = 58/213 (27%), Positives = 95/213 (44%), Gaps = 17/213 (7%) Query: 43 SVTIGRFQSVRHDVDIEKARELNIPVVRRITGGGSVFHDEFGEITYSVVISDDYHPSLKN 102 +V IGR Q+ + ++ + + + RR TGGG+VFHD G ++ + P Sbjct: 43 TVVIGRAQNPWRECKTDRMEQDKVKLARRQTGGGAVFHD-LGNTNFTFMAGK---PEYDK 98 Query: 103 IQESYRFLAGPLVDALKDLGLNAGFSGLNDIVVNG----KKISGSAQTRRKGIILQHGTF 158 + LAG L+ LG++ +G ND+V+ +K SGSA HGT Sbjct: 99 EVSTKIVLAG-----LQKLGIHGVANGRNDLVLEDEQGIRKFSGSAYRETLDRGFHHGTL 153 Query: 159 MYSTRLEVLASVLKVSKEKLRDKGIRSIYERVTTLEREGIKLSMQETYELLRNSFFRAFP 218 + S L LA L +KL+ KGI S+ RV L + Q+ E + ++ + Sbjct: 154 LLSADLNRLADYLNPDLKKLQAKGITSVKSRVINLNTVKADIEHQQVCEAIMQAYCEHYQ 213 Query: 219 LE-EGELTEYELELAQELVEERYGKD---EWNF 247 + E EL + E+++ + +WNF Sbjct: 214 QQVEPELISPQSFFDLPGFEQKFAQQSSWDWNF 246 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.319 0.139 0.384 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 85951599 Number of Sequences: 2977 Number of extensions: 3415414 Number of successful extensions: 8873 Number of sequences better than 1.0e-10: 27 Number of HSP's better than 0.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 8807 Number of HSP's gapped (non-prelim): 27 length of query: 249 length of database: 189,106,746 effective HSP length: 57 effective length of query: 192 effective length of database: 154,992,987 effective search space: 29758653504 effective search space used: 29758653504 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 164 (68.3 bits)