BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB1991 (phnP) DE:ATP-BINDING PROTEIN PHNP (PHNP) (249 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||H75174 ATP-binding protein phnp (phnp) PAB1991 - Pyrococcus... 518 e-146 pir||G71039 probable PHNP protein - Pyrococcus horikoshii >gi|32... 432 e-120 gi|11498089 ATP-binding protein PhnP (phnP) [Archaeoglobus fulgi... 103 2e-21 sp|P16692|PHNP_ECOLI PHNP PROTEIN >gi|96286|pir||H35719 phnP pro... 101 9e-21 pir||F83223 conserved hypothetical protein PA3372 [imported] - P... 88 9e-17 pir||E71450 hypothetical protein PH0260 - Pyrococcus horikoshii ... 85 1e-15 pir||D70166 phnP protein (phnP) homolog - Lyme disease spirochet... 82 5e-15 pir||E71649 hypothetical protein RP869 - Rickettsia prowazekii >... 69 7e-11 >pir||H75174 ATP-binding protein phnp (phnp) PAB1991 - Pyrococcus abyssi (strain Orsay) >gi|5457989|emb|CAB49479.1| (AJ248284) ATP-BINDING PROTEIN PHNP (PHNP) [Pyrococcus abyssi] Length = 249 Score = 518 bits (1319), Expect = e-146 Identities = 249/249 (100%), Positives = 249/249 (100%) Query: 1 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIRE 60 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIRE Sbjct: 1 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIRE 60 Query: 61 FLNTPIDAIFLTHWHHDHIYGLYKLRWIARETELYAPRGHADALILQDPKNLKPNIIRAG 120 FLNTPIDAIFLTHWHHDHIYGLYKLRWIARETELYAPRGHADALILQDPKNLKPNIIRAG Sbjct: 61 FLNTPIDAIFLTHWHHDHIYGLYKLRWIARETELYAPRGHADALILQDPKNLKPNIIRAG 120 Query: 121 DKIKINGLKITAVKLNHQVETLGYIIEENGKNVAVLYDTKGLPRETEIILEKLSPLRLAI 180 DKIKINGLKITAVKLNHQVETLGYIIEENGKNVAVLYDTKGLPRETEIILEKLSPLRLAI Sbjct: 121 DKIKINGLKITAVKLNHQVETLGYIIEENGKNVAVLYDTKGLPRETEIILEKLSPLRLAI 180 Query: 181 VDATYPPGFNDPYHNNVDEAGEMGIKYAERVVLSHISHKNLPFLELVNYVRRKWDDRVLV 240 VDATYPPGFNDPYHNNVDEAGEMGIKYAERVVLSHISHKNLPFLELVNYVRRKWDDRVLV Sbjct: 181 VDATYPPGFNDPYHNNVDEAGEMGIKYAERVVLSHISHKNLPFLELVNYVRRKWDDRVLV 240 Query: 241 AYDGMVFYV 249 AYDGMVFYV Sbjct: 241 AYDGMVFYV 249 >pir||G71039 probable PHNP protein - Pyrococcus horikoshii >gi|3258036|dbj|BAA30719.1| (AP000006) 249aa long hypothetical PHNP protein [Pyrococcus horikoshii] Length = 249 Score = 432 bits (1099), Expect = e-120 Identities = 200/249 (80%), Positives = 223/249 (89%) Query: 1 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIRE 60 MI+YFIGTGGSEGIPVHLC+C +CNEARKF FAQRRPSTLA+IG GEV+L DVGTDIRE Sbjct: 1 MIIYFIGTGGSEGIPVHLCDCNTCNEARKFRFAQRRPSTLAVIGENGEVILFDVGTDIRE 60 Query: 61 FLNTPIDAIFLTHWHHDHIYGLYKLRWIARETELYAPRGHADALILQDPKNLKPNIIRAG 120 FLN P+DAIFLTHWHHDHIYGLYKLRWIARET LYAP GHADALILQDPKNL+P II+AG Sbjct: 61 FLNVPLDAIFLTHWHHDHIYGLYKLRWIARETNLYAPEGHADALILQDPKNLRPKIIKAG 120 Query: 121 DKIKINGLKITAVKLNHQVETLGYIIEENGKNVAVLYDTKGLPRETEIILEKLSPLRLAI 180 +KI+I +K+T+VKLNHQ+ETLGYIIEE+GK VA+LYDTKGLP ET LE +SPLR+AI Sbjct: 121 EKIRIGDIKVTSVKLNHQIETLGYIIEEDGKRVAILYDTKGLPPETREYLEDISPLRVAI 180 Query: 181 VDATYPPGFNDPYHNNVDEAGEMGIKYAERVVLSHISHKNLPFLELVNYVRRKWDDRVLV 240 VDATYPPGF DPYHNNV+EA EM I AER+VLSHISHKNLPFL+LVNY R+ W +VLV Sbjct: 181 VDATYPPGFVDPYHNNVNEAVEMSIDIAERIVLSHISHKNLPFLKLVNYTRKMWGGKVLV 240 Query: 241 AYDGMVFYV 249 AYDGMVFYV Sbjct: 241 AYDGMVFYV 249 >gi|11498089 ATP-binding protein PhnP (phnP) [Archaeoglobus fulgidus] >gi|7482914|pir||F69309 ATP-binding protein PhnP (phnP) homolog - Archaeoglobus fulgidus >gi|2650153|gb|AAB90764.1| (AE001071) ATP-binding protein PhnP (phnP) [Archaeoglobus fulgidus] Length = 254 Score = 103 bits (254), Expect = 2e-21 Identities = 75/225 (33%), Positives = 112/225 (49%), Gaps = 12/225 (5%) Query: 1 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIRE 60 MIV +GTG S G P+ C+CR+C +AR+ G+ ++R S + + N G+ VLID D+R Sbjct: 1 MIVTLLGTGDSPGTPILNCHCRTCEDARRKGWERKRFSVM--VQNNGKTVLIDTSPDLRR 58 Query: 61 FL----NTPIDAIFLTHWHHDHIYGLYKLRWIARETELYA-PRGHADALILQDPKNLKPN 115 L +DA+ TH H DH G + + ++Y+ P+ H D + KP Sbjct: 59 QLLDCGVEKVDAVIWTHCHFDHFGGFGEFYRVQNNVKVYSTPQIHEDIGHFMKFMSYKPV 118 Query: 116 IIRAGDKIKINGLKITAVKLNH-QVETLGYIIEENGKNVAVLYDTKGLPRETEIILEKLS 174 + A + I G+ T + +NH V +G +E G V V DT R E L ++ Sbjct: 119 EVEAFENFTIAGMDFTLLPVNHPPVSAVGVKVEWKGYKVVVSGDTN--VRIPEATLREMQ 176 Query: 175 PLRLAIVDATYPPGFNDPYHNNVDEAGEMGIKY-AERVVLSHISH 218 L IV+A P G H N EA E+ + A +VVL+H+ H Sbjct: 177 NPDLLIVEALAPTG-RFRKHMNAKEALELAKRLNAGKVVLTHLGH 220 >sp|P16692|PHNP_ECOLI PHNP PROTEIN >gi|96286|pir||H35719 phnP protein - Escherichia coli >gi|147213|gb|AAA24356.1| (J05260) phnP protein [Escherichia coli] >gi|216605|dbj|BAA14276.1| (D90227) phnP [Escherichia coli] >gi|536936|gb|AAA96991.1| (U14003) phnP gene product [Escherichia coli] >gi|1790530|gb|AAC77053.1| (AE000482) phosphonate metabolism [Escherichia coli K12] Length = 252 Score = 101 bits (249), Expect = 9e-21 Identities = 69/226 (30%), Positives = 113/226 (49%), Gaps = 21/226 (9%) Query: 7 GTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIREFLNTP- 65 GTGG++G+P C C +C AR+ +R+P + ++ + LID G +P Sbjct: 9 GTGGAQGVPAWGCECAACARARRSPQYRRQPCS-GVVKFNDAITLIDAGLHDLADRWSPG 67 Query: 66 -IDAIFLTHWHHDHIYGLYKLRW-IARETELYAP---RGHADAL----ILQDPKNLKPNI 116 LTH+H DH+ GL+ LRW + +Y P +G D +L ++P + Sbjct: 68 SFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPPDEQGCDDLFKHPGLLDFSHTVEPFV 127 Query: 117 IRAGDKIKINGLKITAVKLNHQVETLGYIIEENGKNVAVLYDTKGLPRETEIILEKLSPL 176 + + GL++T + LNH T GY++E VA L DT GLP +T L P Sbjct: 128 V-----FDLQGLQVTPLPLNHSKLTFGYLLETAHSRVAWLSDTAGLPEKTLKFLRNNQP- 181 Query: 177 RLAIVDATYPPGFNDPYHNNVDEAGEMGIKY---AERVVLSHISHK 219 ++ ++D ++PP + P N+ D + + + RV+L+HISH+ Sbjct: 182 QVMVMDCSHPPRADAP-RNHCDLNTVLALNQVIRSPRVILTHISHQ 226 >pir||F83223 conserved hypothetical protein PA3372 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9949507|gb|AAG06760.1|AE004759_7 (AE004759) conserved hypothetical protein [Pseudomonas aeruginosa] Length = 256 Score = 88.2 bits (215), Expect = 9e-17 Identities = 78/233 (33%), Positives = 115/233 (48%), Gaps = 20/233 (8%) Query: 1 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVG-TDIR 59 M + +GTG + +PV+ C+C +C AR +RRP + A++ G+ LID G D+ Sbjct: 1 MRLTLLGTGDARQVPVYGCDCPACRNARADAGLRRRPCS-ALLECAGQRWLIDSGLVDLC 59 Query: 60 E-FLNTPIDAIFLTHWHHDHIYGLYKLRWIARETELYAPRGHADALILQDPKNLKPNII- 117 E F +D I TH+H DH GL LRW + + G AD L D P I+ Sbjct: 60 ERFPPHSLDGILQTHYHADHAQGLLHLRW--GQGLVIPVHGPADPEGLADLYK-HPGILD 116 Query: 118 -----RAGDKIKINGLKITAVKLNHQVETLGYIIE---ENG--KNVAVLYDTKGLPRETE 167 A ++ + L TA+ L H T GY++E E G + +A L DT GLP ++ Sbjct: 117 FSQPFGAFERRQWGALAATALPLVHSKPTFGYLLEGASEEGTIRRLAYLTDTVGLPADSA 176 Query: 168 IILEKLSPLRLAIVDATYPPGFNDP-YHNNVDEAGE-MGIKYAERVVLSHISH 218 L +PL L ++D + PP P HN++ A E + R VL+H+ H Sbjct: 177 SRL-LAAPLDLLVLDCSMPPQERPPRNHNDLTRALECIESLRPRRAVLTHVGH 228 >pir||E71450 hypothetical protein PH0260 - Pyrococcus horikoshii >gi|3256649|dbj|BAA29332.1| (AP000001) 257aa long hypothetical protein [Pyrococcus horikoshii] Length = 257 Score = 84.7 bits (206), Expect = 1e-15 Identities = 71/240 (29%), Positives = 116/240 (47%), Gaps = 27/240 (11%) Query: 1 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIR- 59 M V +G+G G P LC C +C AR +R ++ I G +LID G D+ Sbjct: 1 MKVIILGSGSYSGTPKPLCTCENCTRARINPAFRRTRFSVYIEGG----ILIDPGPDLHY 56 Query: 60 --EFLNTPIDAIFLTHWHHDHIYGLYKLRWIARETELYAPRG------------HADALI 105 E LN ++ I +TH H DHI+GL + + T P G +++A + Sbjct: 57 HLEKLNKEVETILITHAHFDHIFGLPDAQVFKKLTIASNPLGIKVARSLAMLAFNSEAPL 116 Query: 106 LQDPKNLKPNIIRAGDKIKINGLKITAVKLNHQVETL-GYIIEENGKNVAVLYDT-KGLP 163 D ++ ++ ++ K+NG+K+ + H +E G++IE K VA+ DT + Sbjct: 117 GNDWDYVE---LKFWNEYKVNGVKVIHFPVVHSIEMAGGFLIERKNKTVAITGDTGPEII 173 Query: 164 RETEIILEKLSPLRLAIVDATYPPGFNDPYHNNVDEAGEMG-IKYAERVVLSHISHKNLP 222 R++E+I + + L I + T+ H V++A E+ I AE+ V +HISH N P Sbjct: 174 RDSEVI-DLIKGSDLLISEMTHKESI-PKTHLGVNDAIELAKIVKAEQTVFAHISHSNYP 231 >pir||D70166 phnP protein (phnP) homolog - Lyme disease spirochete >gi|2688455|gb|AAC66903.1| (AE001155) phnP protein (phnP) [Borrelia burgdorferi] Length = 253 Score = 82.3 bits (200), Expect = 5e-15 Identities = 76/269 (28%), Positives = 122/269 (45%), Gaps = 36/269 (13%) Query: 1 MIVYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIRE 60 M+ F+GTG S G+P+ C+C+ C + + +R S+ + + G +LID G DIR+ Sbjct: 1 MVGTFLGTGASSGVPMLNCSCKVCTSS--YSKNKRLRSSFFLESSSGIKLLIDTGPDIRQ 58 Query: 61 FLNTP----IDAIFLTHWHHDHIYGLYKLRWIAR--ETELYA------------PRGHAD 102 L +D + TH H+DHI G +++ R +YA P Sbjct: 59 QLLREKIDRLDLVLYTHEHYDHIMGFDDIKFYTRCAPLNIYARDTAMAHIRNAFPYNFTS 118 Query: 103 ALILQDPKNLKPNIIRAGDKIKINGLKITAVKLNH-QVETLGYIIEENGKNVAVLYDTKG 161 L ++ N+IR + LKI + L H + +LGY + N+A L D K Sbjct: 119 KPSLSGKADIIANVIRDFEPFFFKSLKIVPIPLIHGDIISLGYRV----GNLAYLTDVKF 174 Query: 162 LPRETEIILEKLSPLRLAIVDATYPPGFNDPYHNNVDEAGEMGIKYAERV-VLSHISHKN 220 +P E + L L L I+DA P H N EA + K ++ +HI+H Sbjct: 175 IP---EASYDYLKNLDLLIIDAMRIK--PHPAHLNFSEAFCVIKKINPKISYFTHIAHDI 229 Query: 221 LPFLELVNYVRRKWDDRVLVAYDGMVFYV 249 + E +Y+ + D + +AYDG+ Y+ Sbjct: 230 MH--EEFDYLEK---DNIYLAYDGLKIYI 253 >pir||E71649 hypothetical protein RP869 - Rickettsia prowazekii >gi|3861394|emb|CAA15293.1| (AJ235273) unknown [Rickettsia prowazekii] Length = 249 Score = 68.7 bits (165), Expect = 7e-11 Identities = 69/263 (26%), Positives = 113/263 (42%), Gaps = 33/263 (12%) Query: 3 VYFIGTGGSEGIPVHLCNCRSCNEARKFGFAQRRPSTLAIIGNKGEVVLIDVGTDIREFL 62 V +G G S G+PV C C +C K+ +R + I ++ +L+D G DIR L Sbjct: 4 VTVLGCGASIGVPVIGCECSTCTSPSKY---NKRTRSAIYINDENSQILVDFGFDIRNQL 60 Query: 63 ----NTPIDAIFLTHWHHDHIYGLYKLRWIA----RETELYAPRGHADAL------ILQD 108 +D LTH H DH+ G+ LR + E+Y L + Sbjct: 61 LREKINKLDCAILTHGHSDHVNGIDDLRVFTFMQDKPFEIYTDYNSVAQLHAKFDYLFNL 120 Query: 109 PKNLKPNIIRAGDKIKINGLKITAVKLNH-QVETLGYIIEENGKNVAVLYDTKGLPRETE 167 K L I DKIKIN +++ + +H +++LG I + D G P E+E Sbjct: 121 CKLLATKSINFFDKIKINTIEVQFFRQHHGPIDSLGLRIGD----FVYSPDVIGFPPESE 176 Query: 168 IILEKLSPLRLAIVDATYPPGFNDPYHNNVDEAGEMGIKY-AERVVLSHISHKNLPFLEL 226 L+ + L +D ++P H +D+ KY E ++L+++ H + + E Sbjct: 177 KFLKDIKIWILDCMDYR-----SNPNHAGLDKVLAWREKYNPEEILLTNMRH-TIDYHE- 229 Query: 227 VNYVRRKWDDRVLVAYDGMVFYV 249 +++ + V YDG F V Sbjct: 230 ---IKKVLPNNVKPLYDGYKFMV 249 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.322 0.143 0.435 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 100897886 Number of Sequences: 2977 Number of extensions: 4365428 Number of successful extensions: 9768 Number of sequences better than 1.0e-10: 8 Number of HSP's better than 0.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 9748 Number of HSP's gapped (non-prelim): 8 length of query: 249 length of database: 189,106,746 effective HSP length: 51 effective length of query: 198 effective length of database: 158,583,909 effective search space: 31399613982 effective search space used: 31399613982 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.9 bits) S2: 164 (68.3 bits)