BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2030 (fum-1) DE:fumarase (fum-1)
(271 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75166 fumarase (fum-1) PAB2030 - Pyrococcus abyssi (strain... 543 e-153
pir||C71049 probable L(+)-tartrate dehydratase - Pyrococcus hori... 443 e-123
sp|Q58690|FUMA_METJA PUTATIVE FUMARATE HYDRATASE ALPHA SUBUNIT (... 240 1e-62
pir||A69099 fumarate hydratase, class I - Methanobacterium therm... 237 9e-62
gi|11498699 fumarase (fum-2) [Archaeoglobus fulgidus] >gi|743704... 226 2e-58
pir||D70453 fumarate hydratase (fumarase) - Aquifex aeolicus >gi... 193 2e-48
pir||QQECRT L(+)-tartrate dehydratase (EC 4.2.1.32), iron-depend... 168 5e-41
sp|P05847|TTDA_ECOLI L(+)-TARTRATE DEHYDRATASE ALPHA SUBUNIT (L-... 168 9e-41
dbj|BAB04613.1| (AP001510) fumarate hydratase class I (fumarase)... 156 3e-37
emb|CAA10329.1| (AJ131242) fumarate hydratase B, alpha subunit [... 152 6e-36
sp|Q04718|FUMA_BACST FUMARATE HYDRATASE CLASS I, AEROBIC (FUMARA... 146 2e-34
pir||A72364 fumarate hydratase, N-terminal subunit - Thermotoga ... 143 3e-33
pir||C82216 probable fumarate hydratase, class I VC1304 [importe... 129 3e-29
pir||A81807 fumarate hydratase (EC 4.2.1.2) NMA1812 [imported] -... 127 1e-28
pir||C81063 fumarate hydratase, class I NMB1613 [imported] - Nei... 125 7e-28
pir||A83105 probable fumarase PA4333 [imported] - Pseudomonas ae... 120 2e-26
pir||G82630 fumarate hydratase XF1855 [imported] - Xylella fasti... 111 1e-23
gb|AAA72573.1| (M16194) [Escherichia coli rpsU gene encoding rib... 102 4e-21
pir||H69228 fumarate hydratase, class I related protein - Methan... 89 6e-17
sp|P14407|FUMB_ECOLI FUMARATE HYDRATASE CLASS I, ANAEROBIC (FUMA... 81 1e-14
gb|AAA97022.1| (U14003) fumarase [Escherichia coli] 81 1e-14
pir||B44511 fumarate hydratase (EC 4.2.1.2) fumB, iron-dependent... 81 1e-14
sp|P00923|FUMA_ECOLI FUMARATE HYDRATASE CLASS I, AEROBIC (FUMARA... 81 2e-14
emb|CAC05889.1| (AL391754) fumarate hydratase class I [Streptomy... 70 2e-11
>pir||C75166 fumarase (fum-1) PAB2030 - Pyrococcus abyssi (strain Orsay)
>gi|5457920|emb|CAB49410.1| (AJ248284) fumarase (fum-1)
[Pyrococcus abyssi]
Length = 271
Score = 543 bits (1383), Expect = e-153
Identities = 271/271 (100%), Positives = 271/271 (100%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQD 60
MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQD
Sbjct: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQD 60
Query: 61 TGTPIFFVKTREINEVYEEIVEGVRMATERVPLRPNALRVIDGKVVGNVPEVHFEPGDET 120
TGTPIFFVKTREINEVYEEIVEGVRMATERVPLRPNALRVIDGKVVGNVPEVHFEPGDET
Sbjct: 61 TGTPIFFVKTREINEVYEEIVEGVRMATERVPLRPNALRVIDGKVVGNVPEVHFEPGDET 120
Query: 121 KISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGIASPAEK 180
KISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGIASPAEK
Sbjct: 121 KISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGIASPAEK 180
Query: 181 ALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALDVKVEVETRH 240
ALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALDVKVEVETRH
Sbjct: 181 ALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALDVKVEVETRH 240
Query: 241 PASYIAGVAIQCWAHRRVFLEVKGGRVRIWQ 271
PASYIAGVAIQCWAHRRVFLEVKGGRVRIWQ
Sbjct: 241 PASYIAGVAIQCWAHRRVFLEVKGGRVRIWQ 271
>pir||C71049 probable L(+)-tartrate dehydratase - Pyrococcus horikoshii
>gi|3258112|dbj|BAA30795.1| (AP000006) 272aa long
hypothetical L(+)-tartrate dehydratase [Pyrococcus
horikoshii]
Length = 272
Score = 443 bits (1126), Expect = e-123
Identities = 214/272 (78%), Positives = 243/272 (88%), Gaps = 1/272 (0%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQD 60
MRE+I E I++AVT IP+DVV AI++AL REES++AK+NL MILKAI+I ++EGIPVCQD
Sbjct: 1 MREYIKEGIKLAVTNIPEDVVSAIREALEREESKIAKYNLEMILKAIDISRKEGIPVCQD 60
Query: 61 TGTPIFFVKTR-EINEVYEEIVEGVRMATERVPLRPNALRVIDGKVVGNVPEVHFEPGDE 119
TGTPIFFV+T IN++Y+EIV+GV +AT VPLRPNAL V+DGKV+GNVPEVHFEPG E
Sbjct: 61 TGTPIFFVRTNGPINKIYDEIVKGVEIATREVPLRPNALHVLDGKVIGNVPEVHFEPGKE 120
Query: 120 TKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGIASPAE 179
T ISILMKGGGSENCTALF LTP EGLEG+K KVIEHV+SCGGKPCPPIIVGIGIASPAE
Sbjct: 121 TLISILMKGGGSENCTALFPLTPAEGLEGVKNKVIEHVQSCGGKPCPPIIVGIGIASPAE 180
Query: 180 KALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALDVKVEVETR 239
KA+ILAKKALLR+I ER+ NEKLA FE ELLEEINSLGIGPMGLGG+TTALDVKVEVETR
Sbjct: 181 KAIILAKKALLRRISERHPNEKLAKFERELLEEINSLGIGPMGLGGRTTALDVKVEVETR 240
Query: 240 HPASYIAGVAIQCWAHRRVFLEVKGGRVRIWQ 271
HPASYI GV IQCWAHRR F++V G V+IWQ
Sbjct: 241 HPASYIVGVVIQCWAHRRAFIKVSEGGVKIWQ 272
>sp|Q58690|FUMA_METJA PUTATIVE FUMARATE HYDRATASE ALPHA SUBUNIT (FUMARASE)
>gi|2127927|pir||E64461 fumarate hydratase (EC 4.2.1.2),
class I ' - Methanococcus jannaschii
>gi|1591932|gb|AAB99301.1| (U67570) tartrate
dehydratase, subunit alpha (ttdA) [Methanococcus
jannaschii]
Length = 285
Score = 240 bits (606), Expect = 1e-62
Identities = 130/274 (47%), Positives = 178/274 (64%), Gaps = 15/274 (5%)
Query: 5 IVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTGTP 64
+VE R A +P+DV A+++A ++E SE++K L I++ +I + +P+CQDTG P
Sbjct: 7 VVELFREAAIYLPEDVKNALEEAYKKESSEISKNTLKAIIENNKIAEETQVPLCQDTGVP 66
Query: 65 IFFVK------TREINEVYEEIVEGVRMATERVPLRPNALRVIDGK-----VVGNVPEVH 113
I F+K + EI ++ EEI EGV+ ATE VPLRPN + + + V N P ++
Sbjct: 67 IVFLKIGKNINSSEIMKIIEEIKEGVKKATEEVPLRPNVVHPLTRENFKTNVGLNSPFIN 126
Query: 114 FEPGD----ETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPII 169
E + E +I KG GSEN +AL L P +G+EG+K V+E + + GGKPCPPI+
Sbjct: 127 IEFDESLDREIEIIAFPKGAGSENMSALKMLKPSDGIEGIKNFVLETIANAGGKPCPPIV 186
Query: 170 VGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTA 229
VGIGI A+ AL LAKKALLRKIGER+ ++++A E+ELLE+INSLGIG MGLGG TA
Sbjct: 187 VGIGIGGTADVALKLAKKALLRKIGERHRDKEIANLEKELLEKINSLGIGAMGLGGDITA 246
Query: 230 LDVKVEVETRHPASYIAGVAIQCWAHRRVFLEVK 263
LDV +E+ H AS G+ IQCWA RR +K
Sbjct: 247 LDVFIEIAGCHTASLPVGICIQCWADRRAIKRIK 280
>pir||A69099 fumarate hydratase, class I - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622866|gb|AAB86205.1| (AE000929)
fumarate hydratase, class I [Methanobacterium
thermoautotrophicum]
Length = 281
Score = 237 bits (599), Expect = 9e-62
Identities = 126/266 (47%), Positives = 166/266 (62%), Gaps = 9/266 (3%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQD 60
+ E + R AV RIP DV AIK A +EE E+A NL IL IE+ ++ +PVCQD
Sbjct: 7 VEETVCRLFREAVIRIPPDVTFAIKKAYLQEEDEIALLNLKAILDNIELAEKMEVPVCQD 66
Query: 61 TGTPIFFVKTR--EINEVYEEIVEGVRMATERVPLRPNALRVIDGKVVGN-----VPEVH 113
TG PI FV+ E+ ++YE I GV AT VPLRPN + + + G +P++
Sbjct: 67 TGLPIVFVRMGKVEVEDLYEGIAAGVERATAEVPLRPNVVDPLTRENTGTNTGQMIPQID 126
Query: 114 FE--PGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVG 171
E D +I++ KG GSEN AL PG+G+EG+K V+ V GGKPCPP+IVG
Sbjct: 127 VEITDTDNLEITVFPKGFGSENNNALLMGLPGDGIEGIKDFVVRTVLEAGGKPCPPVIVG 186
Query: 172 IGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALD 231
+GI ++ A+ LAKKALL ++G+RN ++KLA EE++L IN G GPMG+GGKTTALD
Sbjct: 187 VGIGGSSDLAMKLAKKALLERVGDRNPDKKLASLEEDILNSINESGSGPMGMGGKTTALD 246
Query: 232 VKVEVETRHPASYIAGVAIQCWAHRR 257
VK++ H A GV IQCWA RR
Sbjct: 247 VKIKTAHTHTAGLPVGVCIQCWAARR 272
>gi|11498699 fumarase (fum-2) [Archaeoglobus fulgidus] >gi|7437045|pir||B69387
fumarase (fum-2) homolog - Archaeoglobus fulgidus
>gi|2649507|gb|AAB90147.1| (AE001028) fumarase (fum-2)
[Archaeoglobus fulgidus]
Length = 272
Score = 226 bits (570), Expect = 2e-58
Identities = 130/266 (48%), Positives = 174/266 (64%), Gaps = 18/266 (6%)
Query: 3 EHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTG 62
E ++ A++ A T +P+DVV AIK A EE+++AK NL ILK IE K+ G+P+CQDTG
Sbjct: 8 EAVISALKKAHTELPEDVVTAIKKAYDSEENDIAKRNLEAILKNIEAAKKLGVPMCQDTG 67
Query: 63 TPIFFVKT-REI---NEVYEEIVEGVRMATERVPLRPNALRVIDGKVVGN-----VPEVH 113
P+FFV+ RE+ ++ E IVEGVR AT +PLRPNA+ I + G+ +P+++
Sbjct: 68 IPVFFVEVGRELCLDFDLKEAIVEGVRKATAEIPLRPNAVHPITRENSGDNTGLGIPQIN 127
Query: 114 FE--PGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVG 171
E GDE ++ ++ KG GSEN +AL + P + ++ +K V+E VK+ GGKPCPPI VG
Sbjct: 128 VELVKGDELRMVVMPKGAGSENVSALKMMLPTQ-VDKIKDFVVETVKNAGGKPCPPIFVG 186
Query: 172 IGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALD 231
+G+ S + A LAKKALLR + E N+ FE ELLE +N LGIG MGLGGK TAL
Sbjct: 187 VGVGSTFDGAAKLAKKALLRNVLEMND------FELELLEAVNELGIGAMGLGGKYTALA 240
Query: 232 VKVEVETRHPASYIAGVAIQCWAHRR 257
V VE H AS V IQCWA+RR
Sbjct: 241 VLVEFGHCHTASLPVAVNIQCWANRR 266
>pir||D70453 fumarate hydratase (fumarase) - Aquifex aeolicus
>gi|2984079|gb|AAC07618.1| (AE000756) fumarate hydratase
(fumarase) [Aquifex aeolicus]
Length = 281
Score = 193 bits (486), Expect = 2e-48
Identities = 119/267 (44%), Positives = 158/267 (58%), Gaps = 11/267 (4%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQD 60
++E + EAI A +P +V A ++AL+REES + + L IL+ EI ++E +P CQD
Sbjct: 9 IKEAVKEAIIEANCVLPREVRVAFQEALKREESPIGREVLTQILENAEIAQKEKMPYCQD 68
Query: 61 TGTPIFFVKTREINEVY-----EEIVEGVRMATERVPLRPNAL--RVIDGK-VVGNVPEV 112
TG + FV + V + I +GVR ATE LR + + V + K N P +
Sbjct: 69 TGVDVIFVFLGQDVHVVGGSLEDAINQGVREATEEGYLRASMVWDPVFERKNTKDNTPAI 128
Query: 113 -HFE--PGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPII 169
H+E PGD KI + KG GSEN + L L P +G EG+K+ ++E VK G CPP
Sbjct: 129 IHYEVVPGDRVKIVVAPKGAGSENTSRLAMLKPADGWEGVKKFILETVKFAGPNACPPFT 188
Query: 170 VGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTA 229
VG+GI E LAKKALLR +G+R+EN +A EEELLEEIN +G GPMG GG TA
Sbjct: 189 VGVGIGGNFEYCAYLAKKALLRPVGKRHENPLIAKVEEELLEEINKIGWGPMGFGGTVTA 248
Query: 230 LDVKVEVETRHPASYIAGVAIQCWAHR 256
+DVKVE+ H AS V IQC A+R
Sbjct: 249 VDVKVEMYPCHIASLPVAVNIQCHANR 275
>pir||QQECRT L(+)-tartrate dehydratase (EC 4.2.1.32), iron-dependent, alpha
chain - Escherichia coli >gi|882584|gb|AAA89141.1|
(U28379) tartrate dehydratse [Escherichia coli]
>gi|1789442|gb|AAC76097.1| (AE000388) L-tartrate
dehydratase, subunit A [Escherichia coli K12]
Length = 303
Score = 168 bits (422), Expect = 5e-41
Identities = 101/273 (36%), Positives = 148/273 (53%), Gaps = 20/273 (7%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMIL----KAIEIGKREGIP 56
+ E + M TR+PDDVV+ +K E S + K + + KAI++ + P
Sbjct: 14 LTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNMQKAIDLNR----P 69
Query: 57 VCQDTGTPIFFVKTRE----INEVYEEIVEGVRMATERVPLRPNALRVID----GKVVGN 108
CQDTG +FFVK + E+ + + V AT + PLR NA+ + D GK G+
Sbjct: 70 ACQDTGEIMFFVKVGSRFPLLGELQSILKQAVEEATVKAPLRHNAVEIFDEVNTGKNTGS 129
Query: 109 -VPEVHFE---PGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKP 164
VP V ++ D+ +I + M GGG L P EG EG+ + V E++ +
Sbjct: 130 GVPWVTWDIIPDNDDAEIEVYMAGGGCTLPGRSKVLMPSEGYEGVVKFVFENISTLAVNA 189
Query: 165 CPPIIVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLG 224
CPP++VG+GIA+ E A +L++KA+LR IG R+ N K A E L E +N LGIGP GL
Sbjct: 190 CPPVLVGVGIATSVETAAVLSRKAILRPIGSRHPNPKAAELELRLEEGLNRLGIGPQGLT 249
Query: 225 GKTTALDVKVEVETRHPASYIAGVAIQCWAHRR 257
G ++ + V +E RHP++ V+ CWAHRR
Sbjct: 250 GNSSVMGVHIESAARHPSTIGVAVSTGCWAHRR 282
>sp|P05847|TTDA_ECOLI L(+)-TARTRATE DEHYDRATASE ALPHA SUBUNIT (L-TTD ALPHA)
>gi|347084|gb|AAA03061.1| (L14781) tartrate dehydratase
[Escherichia coli]
Length = 303
Score = 168 bits (420), Expect = 9e-41
Identities = 100/273 (36%), Positives = 148/273 (53%), Gaps = 20/273 (7%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMIL----KAIEIGKREGIP 56
+ E + M TR+PDDVV+ +K E S + K + + KAI++ + P
Sbjct: 14 LTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNMQKAIDLNR----P 69
Query: 57 VCQDTGTPIFFVKTRE----INEVYEEIVEGVRMATERVPLRPNALRVID----GKVVGN 108
CQDTG +FFVK + E+ + + V AT + PLR NA+ + D GK G+
Sbjct: 70 ACQDTGEIMFFVKVGSRFPLLGELQSILKQAVEEATVKAPLRHNAVEIFDEVNTGKNTGS 129
Query: 109 -VPEVHFE---PGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKP 164
VP V ++ D+ +I + M GGG L P EG EG+ + V E++ +
Sbjct: 130 GVPWVTWDIIPDNDDAEIEVYMAGGGCTLPGRSKVLMPSEGYEGVVKFVFENISTLAVNA 189
Query: 165 CPPIIVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLG 224
CPP++VG+GIA+ E A +L++KA+LR IG R+ N K A + L E +N LGIGP GL
Sbjct: 190 CPPVLVGVGIATSVETAAVLSRKAILRPIGSRHPNPKAAELDVRLEEGLNRLGIGPQGLT 249
Query: 225 GKTTALDVKVEVETRHPASYIAGVAIQCWAHRR 257
G ++ + V +E RHP++ V+ CWAHRR
Sbjct: 250 GNSSVMGVHIESAARHPSTIGVAVSTGCWAHRR 282
>dbj|BAB04613.1| (AP001510) fumarate hydratase class I (fumarase) [Bacillus
halodurans]
Length = 512
Score = 156 bits (390), Expect = 3e-37
Identities = 99/291 (34%), Positives = 151/291 (51%), Gaps = 21/291 (7%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEV-AKFNLNMILKAIEIGKREGIPVCQ 59
++E + + I T +P+DV AI +A +E + A +L+ I + I++ P+CQ
Sbjct: 4 LQESMYQLIVETSTNLPNDVRRAIAEAKAKENAGTRAALSLSTITENIKMADENISPICQ 63
Query: 60 DTGTPIFFVKTRE-IN--EVYEEIVEGVRMATERVPLRPNALRVIDGKVVGN-----VPE 111
DTG P F +K +N E+ + I + AT+ LRPN++ + GK GN P
Sbjct: 64 DTGMPTFEIKVPVGVNQIEMKKAIYAAIEQATKDGKLRPNSVDSLTGKNSGNNLGEGTPV 123
Query: 112 VHFEPGDETKIS--ILMKGGGSENCTALFTLTP--------GEGLEGLKRKVIEHVKSCG 161
+HFE +E I +++KGGG EN ++L G L+G+++ ++ V
Sbjct: 124 IHFEQWEEDYIDARLILKGGGCENKNIQYSLPAELEGLGRAGRDLDGIRKCIMHSVYQAQ 183
Query: 162 GKPCPPIIVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPM 221
G+ C +G+GI + LAK+ L RK+ + N N+ L EE +LE N LGIG M
Sbjct: 184 GQGCSAGFIGVGIGGDRTTSYSLAKEQLFRKVDDVNPNDDLRKLEEYVLENANKLGIGTM 243
Query: 222 GLGGKTTALDVKVEVETRHPASYIAGVAIQCWAHRR--VFLEVKGGRVRIW 270
G GG+ T L KV R PAS+ VA CWA RR V L+ + G ++ W
Sbjct: 244 GFGGEATLLGCKVGAMNRLPASFFVSVAYNCWAFRRLGVELDAETGEIKKW 294
>emb|CAA10329.1| (AJ131242) fumarate hydratase B, alpha subunit [Wolinella
succinogenes]
Length = 281
Score = 152 bits (379), Expect = 6e-36
Identities = 96/268 (35%), Positives = 134/268 (49%), Gaps = 17/268 (6%)
Query: 3 EHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTG 62
E + + + A + D+ A K A E S + K L I++ +I KRE +P+CQDTG
Sbjct: 11 EAVAKLCKEACYYVTPDMYAAFKKAEETEVSPLGKHILGRIIENADIAKREDMPICQDTG 70
Query: 63 TPIFFVKTREINEVYEEIVEGVRMATERVPLRPNALRVIDGKVVG-----------NVPE 111
+ FV+ + ++ +E A LR VVG N P
Sbjct: 71 MTVVFVEIGQEVKIEGGYIEDAINAGVAKGYTEGYLRK---SVVGEPLFNRANTKDNTPA 127
Query: 112 V---HFEPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPI 168
V PGD+ K+ + KG GSEN + L L P +GLEG+K+ +E VK G CPP+
Sbjct: 128 VIHTRIVPGDKLKLKVCPKGFGSENKSVLKMLVPADGLEGVKKVFLEAVKLAGPNACPPM 187
Query: 169 IVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTT 228
++G+GI EKA ILAKKA +R + NE+ A E++LLE + G+GP GLGG T
Sbjct: 188 VIGVGIGGTMEKAAILAKKAAVRSVDSYNEHPDYAQLEKDLLEMACATGVGPQGLGGINT 247
Query: 229 ALDVKVEVETRHPASYIAGVAIQCWAHR 256
A+ V +E H A V I C A R
Sbjct: 248 AVKVNIEWYPTHIAGLPVAVNINCHAAR 275
>sp|Q04718|FUMA_BACST FUMARATE HYDRATASE CLASS I, AEROBIC (FUMARASE)
>gi|477033|pir||A47692 fumarate hydratase (EC 4.2.1.2),
iron-dependent - Bacillus stearothermophilus
>gi|142944|gb|AAA72317.1| (L05611) fumarase [Bacillus
stearothermophilus]
Length = 514
Score = 146 bits (366), Expect = 2e-34
Identities = 97/289 (33%), Positives = 147/289 (50%), Gaps = 21/289 (7%)
Query: 3 EHIVEAIRMAVTRIPDDVVEAIKDALRREESEV-AKFNLNMILKAIEIGKREGIPVCQDT 61
E + + I T++P DV AI A +RE + A +L I + I++ P+CQDT
Sbjct: 6 ESMYQLIVETSTKLPKDVRRAILKAKQRENAGTRAAMSLATITENIKMADENVSPICQDT 65
Query: 62 GTPIFFVKTRE-INEVY--EEIVEGVRMATERVPLRPNALRVIDGKVVGN-----VPEVH 113
G P F +K IN++ E I + + AT+ LRPN++ + G+ G+ +P V
Sbjct: 66 GLPTFKIKVPVGINQIQIKETIKKAIAQATKDGKLRPNSVDSLTGENSGDNLGEGLPVVK 125
Query: 114 FEPG--DETKISILMKGGGSENCTALFTLT--------PGEGLEGLKRKVIEHVKSCGGK 163
FE D + +++KGGG EN ++L G L+G+++ ++ V G+
Sbjct: 126 FEQWEKDYMDVRLILKGGGCENKNIQYSLPCELEGLGRAGRDLDGIRKCILHAVYQAQGQ 185
Query: 164 PCPPIIVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGL 223
C +G+GI LAK+ L R++ + N NE+L EE ++E N LGIG MG
Sbjct: 186 GCSAGFIGVGIGGDRSAGYDLAKEQLFREVDDVNPNEELRQLEEYIMENANKLGIGTMGF 245
Query: 224 GGKTTALDVKVEVETRHPASYIAGVAIQCWAHRR--VFLEVKGGRVRIW 270
GG+TT L KV R PAS+ VA CWA RR V ++ G + W
Sbjct: 246 GGETTLLGCKVGAMHRIPASFFVSVAYNCWAFRRLGVHIDPNTGEIIKW 294
>pir||A72364 fumarate hydratase, N-terminal subunit - Thermotoga maritima
(strain MSB8) >gi|4981054|gb|AAD35625.1|AE001729_5
(AE001729) fumarate hydratase, N-terminal subunit
[Thermotoga maritima]
Length = 272
Score = 143 bits (356), Expect = 3e-33
Identities = 92/268 (34%), Positives = 139/268 (51%), Gaps = 15/268 (5%)
Query: 1 MREHIV-EAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQ 59
+ +H++ E I+ A+ + +++ + R E + ++I + + K E +P+CQ
Sbjct: 2 INKHVISEKIKKAIVEANIKINPEVEEIIERYEGPFS----DIIKENYRVSKEEKLPLCQ 57
Query: 60 DTGTPIFFVKTREINEVYEEIVEGVRMATERV----PLRPNAL------RVIDGKVVGNV 109
DTG FFV ++ E I E + A V P R + + R+ G +
Sbjct: 58 DTGIVEFFVFIGHEVKLEEPIEETLNRAVGEVYSEYPFRYSVVSDPLFERINTGTNTPAI 117
Query: 110 PEVHFEPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPII 169
+ G +I L+KGGGSEN T L +TP +E +K VIEH+K G K CPP+
Sbjct: 118 VHIFQVKGKSLEIRFLVKGGGSENLTVLRMMTPTSDVEKVKEFVIEHIKEHGAKACPPLH 177
Query: 170 VGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTA 229
VGIGI A+KAL+L+K AL + ERN++ K A E+EL +E+N LGIG GLG T
Sbjct: 178 VGIGIGGTADKALLLSKLALTKSFKERNKDPKYAGLEKELEKELNFLGIGFQGLGKGITV 237
Query: 230 LDVKVEVETRHPASYIAGVAIQCWAHRR 257
V +E H A+ V++ C+ RR
Sbjct: 238 YSVHIEHFPTHIATLPVAVSVDCYLCRR 265
>pir||C82216 probable fumarate hydratase, class I VC1304 [imported] - Vibrio
cholerae (group O1 strain N16961)
>gi|9655793|gb|AAF94463.1| (AE004210) fumarate
hydratase, class I, putative [Vibrio cholerae]
Length = 505
Score = 129 bits (322), Expect = 3e-29
Identities = 95/283 (33%), Positives = 136/283 (47%), Gaps = 29/283 (10%)
Query: 5 IVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTGTP 64
+ +A++ P D V+A++ A +REES+ AK ++ IL + P+CQDTG
Sbjct: 13 VADALQYISYYHPLDFVKALEKAYQREESQAAKDSIAQILINSRMSAEGHRPICQDTGIV 72
Query: 65 IFFVKT-------REINEVYEEIVEGVRMATERV--PLRPNALRVIDGKVVGN------V 109
FV V + + EGVR A PLR + L GK + V
Sbjct: 73 TCFVNIGMGVQWDSTDMTVQQMVDEGVRQAYTNPDNPLRASVLMDPAGKRINTKDNTPAV 132
Query: 110 PEVHFEPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPII 169
++ PGD+ I I KGGGSEN T + L P + + K + + G CPP +
Sbjct: 133 VHINMVPGDKVDIQIAAKGGGSENKTKMVMLNPSDDIAEWVEKTVPLM---GAGWCPPGM 189
Query: 170 VGIGIASPAEKALILAKKALLRKI--------GERNENEKLAMFEEELLEEINSLGIGPM 221
+GIGI AEKA +LAK+AL+ I G +N E+L + ++ +N LGIG
Sbjct: 190 LGIGIGGTAEKAAVLAKEALMEHIDIHELIERGPQNAEEELRL---DIFNRVNKLGIGAQ 246
Query: 222 GLGGKTTALDVKVEVETRHPASYIAGVAIQCWAHRRVFLEVKG 264
GLGG TT +DVK++ H AS + C A R V + G
Sbjct: 247 GLGGLTTVVDVKIKSAPTHAASKPVCMIPNCAATRHVHFTLDG 289
>pir||A81807 fumarate hydratase (EC 4.2.1.2) NMA1812 [imported] - Neisseria
meningitidis (group A strain Z2491)
>gi|7380451|emb|CAB85037.1| (AL162757) fumarate
hydratase [Neisseria meningitidis Z2491]
Length = 546
Score = 127 bits (317), Expect = 1e-28
Identities = 98/285 (34%), Positives = 140/285 (48%), Gaps = 26/285 (9%)
Query: 3 EHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTG 62
+ I +A + P D ++A+ A ++EE+ AK + IL + P+CQDTG
Sbjct: 50 QSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAENNRPICQDTG 109
Query: 63 TPIFFVKTR-----EINEVYEEIV-EGVRMAT--ERVPLRPNALRVIDGK---VVGNVPE 111
F+K + + EE+V EGVR A E LR + L GK N P
Sbjct: 110 IATVFLKVGMDVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGKRQNTKDNTPA 169
Query: 112 V---HFEPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPI 168
V PGD+ +++ KGGGSEN + L L P + + V++ + + G CPP
Sbjct: 170 VIHMSIVPGDKVEVTCAAKGGGSENKSKLAMLNPSDNIVDW---VLKTIPTMGAGWCPPG 226
Query: 169 IVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEE---------ELLEEINSLGIG 219
I+GIGI EKA+++AK++L+ I + EK A E EL E++N+LGIG
Sbjct: 227 ILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEKVNALGIG 286
Query: 220 PMGLGGKTTALDVKVEVETRHPASYIAGVAIQCWAHRRVFLEVKG 264
GLGG TT LDVK+ H AS + C A R V E+ G
Sbjct: 287 AQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDG 331
>pir||C81063 fumarate hydratase, class I NMB1613 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7226863|gb|AAF41965.1| (AE002511) fumarate
hydratase, class I [Neisseria meningitidis MC58]
Length = 507
Score = 125 bits (310), Expect = 7e-28
Identities = 97/285 (34%), Positives = 139/285 (48%), Gaps = 26/285 (9%)
Query: 3 EHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTG 62
+ I +A + P D ++A+ A ++EE+ AK + IL + P+CQDTG
Sbjct: 11 QSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAENNRPICQDTG 70
Query: 63 TPIFFVKTR-----EINEVYEEIV-EGVRMAT--ERVPLRPNALRVIDGK---VVGNVPE 111
F+K + + EE+V EGVR A E LR + L GK N P
Sbjct: 71 IATVFLKVGMNVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGKRQNTKDNTPA 130
Query: 112 V---HFEPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPPI 168
V PG + +++ KGGGSEN + L L P + + V++ + + G CPP
Sbjct: 131 VIHMSIVPGGKVEVTCAAKGGGSENKSKLAMLNPSDNIVDW---VLKTIPTMGAGWCPPG 187
Query: 169 IVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEE---------ELLEEINSLGIG 219
I+GIGI EKA+++AK++L+ I + EK A E EL E++N+LGIG
Sbjct: 188 ILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEKVNALGIG 247
Query: 220 PMGLGGKTTALDVKVEVETRHPASYIAGVAIQCWAHRRVFLEVKG 264
GLGG TT LDVK+ H AS + C A R V E+ G
Sbjct: 248 AQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDG 292
>pir||A83105 probable fumarase PA4333 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9950558|gb|AAG07721.1|AE004849_8
(AE004849) probable fumarase [Pseudomonas aeruginosa]
Length = 507
Score = 120 bits (297), Expect = 2e-26
Identities = 96/286 (33%), Positives = 139/286 (48%), Gaps = 31/286 (10%)
Query: 3 EHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTG 62
+ + +A++ P D ++A+ +A REES A+ ++ IL + P+CQDTG
Sbjct: 11 QSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCATGHRPICQDTG 70
Query: 63 TPIFFVKTR-------EINEVYEEIVEGVRMA---TERVPLRPNALRVIDG---KVVGNV 109
FV+ V + I EGVR A E V LR + L G N
Sbjct: 71 IVTVFVRVGMDVRWDGATMSVDDMINEGVRRAYNLPENV-LRASILADPAGARKNTKDNT 129
Query: 110 PEV-HFE--PGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCP 166
P V H+ PGD+ ++ + KGGGSEN + + L P + + V++ V + G CP
Sbjct: 130 PAVIHYSIVPGDKVEVDVAAKGGGSENKSKMAMLNPSDSIVDW---VLKTVPTMGAGWCP 186
Query: 167 PIIVGIGIASPAEKALILAKKALLRKI--------GERNENEKLAMFEEELLEEINSLGI 218
P ++GIGI AEKA ++AK+ L+ I G +N E+L + EL E++N LGI
Sbjct: 187 PGMLGIGIGGTAEKAAVMAKEVLMDPIDIHELKARGPQNRIEELRL---ELFEKVNQLGI 243
Query: 219 GPMGLGGKTTALDVKVEVETRHPASYIAGVAIQCWAHRRVFLEVKG 264
G GLGG TT LDVK+ H AS + C A R + G
Sbjct: 244 GAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDG 289
>pir||G82630 fumarate hydratase XF1855 [imported] - Xylella fastidiosa (strain
9a5c) >gi|9106941|gb|AAF84661.1|AE004006_9 (AE004006)
fumarate hydratase [Xylella fastidiosa]
Length = 551
Score = 111 bits (274), Expect = 1e-23
Identities = 91/285 (31%), Positives = 133/285 (45%), Gaps = 29/285 (10%)
Query: 3 EHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTG 62
+ I +A++ P D ++ + A REES AK + IL + P+CQDTG
Sbjct: 57 QSIADALQYISYYHPVDYIKTLVLAYEREESPAAKDAMAQILINSRMCAEGRRPICQDTG 116
Query: 63 TPIFF------VKTREINEVYEEIV-EGVRMATERVP--LRPNALRVIDGKVVGN----- 108
F V+ ++ EE+V EGVR A LR + L GK
Sbjct: 117 IVTVFLSIGMEVRWDDVTMDVEEMVNEGVRRAYRHPDNTLRASVLADPAGKRCNTRDNTP 176
Query: 109 -VPEVHFEPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKPCPP 167
V V G ++ + KGGGSE + L P + + V++ V + G CPP
Sbjct: 177 AVVNVKVVSGGHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDW---VLDTVPTMGAGWCPP 233
Query: 168 IIVGIGIASPAEKALILAKKALLRKI--------GERNENEKLAMFEEELLEEINSLGIG 219
++GIGI AEKA++LAK+AL+ I G N E+L + EL E++N+LGIG
Sbjct: 234 GMLGIGIGGTAEKAMLLAKEALMEPIDIQDLMVRGPSNRAEELRL---ELYEKVNALGIG 290
Query: 220 PMGLGGKTTALDVKVEVETRHPASYIAGVAIQCWAHRRVFLEVKG 264
G+GG TT LD+KV+ H A+ + C + R + G
Sbjct: 291 AQGVGGVTTVLDIKVKDYPTHAANLPVAMIPNCASTRHAHFTLDG 335
>gb|AAA72573.1| (M16194) [Escherichia coli rpsU gene encoding ribosomal protein
S21, partial cds, with 5' flank encoding three
unidentified proteins, complete cds..], gene products
>gi|225552|prf||1306285A ORF z1,rpsU upstream
[Escherichia coli]
Length = 291
Score = 102 bits (252), Expect = 4e-21
Identities = 71/217 (32%), Positives = 109/217 (49%), Gaps = 21/217 (9%)
Query: 1 MREHIVEAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMIL----KAIEIGKREGIP 56
+ E + M TR+PDDVV+ +K E S + K + + KAI++ + P
Sbjct: 13 LTEIVANFTAMISTRMPDDVVDKLKQLKDAETSSMGKIIYHTMFDNMQKAIDLNR----P 68
Query: 57 VCQDTGTPIFFVKTRE----INEVYEEIVEGVRMATERVPLRPNALRVID----GKVVGN 108
CQDTG +FFVK + E+ + + V AT + PLR NA+ + D GK G+
Sbjct: 69 ACQDTGEIMFFVKVGSRFPLLGELQSILKQAVEEATVKAPLRHNAVEIFDEVNTGKNTGS 128
Query: 109 -VPEVHFE---PGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKSCGGKP 164
VP V ++ D+ +I + M GGG L P EG EG+ + V E++ +
Sbjct: 129 GVPWVTWDIIPDNDDAEIEVYMAGGGCTLPGRSKVLMPSEGYEGVVKFVFENISTLAVNA 188
Query: 165 CPPIIVGIGIASPAEKALILAKKALLRKIGERNENEK 201
CPP++VG+ + E A +L++KA+LR IG K
Sbjct: 189 CPPVLVGVA-SHLGETAAVLSRKAILRPIGRPPSQSK 224
>pir||H69228 fumarate hydratase, class I related protein - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622061|gb|AAB85459.1| (AE000870) fumarate
hydratase, class I related protein [Methanobacterium
thermoautotrophicum]
Length = 280
Score = 88.9 bits (217), Expect = 6e-17
Identities = 75/252 (29%), Positives = 115/252 (44%), Gaps = 30/252 (11%)
Query: 7 EAIRMAVTRIPDDVVEAIKDALRREESEVAKFNLNMILKAIEIGKREGIPVCQDTGTPIF 66
+AI A T DV+ A + AL EE E + + L ++++ I +RE P+C DTG P
Sbjct: 9 DAIIQASTTYSPDVLRAYERALEVEEDERSAWILELLIENARIAERERRPLCDDTGIPHL 68
Query: 67 FVKTREINEV----YEEIVEGVRMATERVPLRPNALRVIDGKVV----------GNVPEV 112
V+ E + + + GV +P RP A+R + + + +P
Sbjct: 69 IVEVGEDASLPAGFFNHLKRGVEEGLRELPGRPMAVRGDEVERIEQSRGLYSDPAKLPPA 128
Query: 113 HF----EPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEHVKS----CGGKP 164
F PG+ +I +LM GGG E + + +VI +K+ G P
Sbjct: 129 PFTVDSAPGEGVRIHVLMLGGGPEIRARTRRIFHRHSYRKVFEEVITWMKTEVPMLGCTP 188
Query: 165 CPPIIVGIGIASPAEKALILAKKALLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLG 224
C P I GIG + A +LR + N +E+ + EE + E +NS G GP+GLG
Sbjct: 189 CIPAI-GIG------RTHFEATGLMLRAMATGNLDEQ-SWIEEHITESLNSTGTGPLGLG 240
Query: 225 GKTTALDVKVEV 236
G TTAL ++V
Sbjct: 241 GSTTALGAMIKV 252
>sp|P14407|FUMB_ECOLI FUMARATE HYDRATASE CLASS I, ANAEROBIC (FUMARASE)
>gi|146048|gb|AAA23827.1| (M27058) anaerobic class I
fumarase (EC 4.2.1.2) [Escherichia coli]
Length = 548
Score = 81.1 bits (197), Expect = 1e-14
Identities = 85/266 (31%), Positives = 120/266 (44%), Gaps = 35/266 (13%)
Query: 31 EESEVAKFNLNMILKAIEIGKREGIPVCQDTGTPIFFVKTREINEVY------EEIVEGV 84
E SE K+ L+ EI + +P CQDTGT I K + V+ E + +GV
Sbjct: 78 EASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQ--RVWTGGGDEETLSKGV 135
Query: 85 RMATERVPLRPNALRVID-GKVVG---NVP---EVHFEPGDETKISILMKGGGSENCTAL 137
LR + +D K V N+P +++ GDE K + KGGGS N T L
Sbjct: 136 YNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYL 195
Query: 138 F-----TLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGI-ASPAEKALILAKKA--- 188
+ LTPG+ LK ++E +++ G CPP + I + AE L K A
Sbjct: 196 YQETKALLTPGK----LKNFLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAH 251
Query: 189 ----LLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALDVKVEVETRHPASY 244
L + E + + E+ELLEE LG+G GGK A D++V RH AS
Sbjct: 252 YYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQ-FGGKYFAHDIRVIRLPRHGASC 310
Query: 245 IAGVAIQCWAHRRVFLEVKGGRVRIW 270
G+ + C A R + + K R IW
Sbjct: 311 PVGMGVSCSADRNI--KAKINREGIW 334
>gb|AAA97022.1| (U14003) fumarase [Escherichia coli]
Length = 548
Score = 81.1 bits (197), Expect = 1e-14
Identities = 85/266 (31%), Positives = 120/266 (44%), Gaps = 35/266 (13%)
Query: 31 EESEVAKFNLNMILKAIEIGKREGIPVCQDTGTPIFFVKTREINEVY------EEIVEGV 84
E SE K+ L+ EI + +P CQDTGT I K + V+ E + +GV
Sbjct: 78 EASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQ--RVWTGGGDEETLSKGV 135
Query: 85 RMATERVPLRPNALRVID-GKVVG---NVP---EVHFEPGDETKISILMKGGGSENCTAL 137
LR + +D K V N+P +++ GDE K + KGGGS N T L
Sbjct: 136 YNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYL 195
Query: 138 F-----TLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGI-ASPAEKALILAKKA--- 188
+ LTPG+ LK ++E +++ G CPP + I + AE L K A
Sbjct: 196 YQETKALLTPGK----LKNFLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAH 251
Query: 189 ----LLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALDVKVEVETRHPASY 244
L + E + + E+ELLEE LG+G GGK A D++V RH AS
Sbjct: 252 YYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQ-FGGKYFAHDIRVIRLPRHGASC 310
Query: 245 IAGVAIQCWAHRRVFLEVKGGRVRIW 270
G+ + C A R + + K R IW
Sbjct: 311 PVGMGVSCSADRNI--KAKINREGIW 334
>pir||B44511 fumarate hydratase (EC 4.2.1.2) fumB, iron-dependent - Escherichia
coli >gi|1790564|gb|AAC77083.1| (AE000485) fumarase B=
fumarate hydratase Class I; anaerobic isozyme
[Escherichia coli K12]
Length = 548
Score = 81.1 bits (197), Expect = 1e-14
Identities = 85/266 (31%), Positives = 120/266 (44%), Gaps = 35/266 (13%)
Query: 31 EESEVAKFNLNMILKAIEIGKREGIPVCQDTGTPIFFVKTREINEVY------EEIVEGV 84
E SE K+ L+ EI + +P CQDTGT I K + V+ E + +GV
Sbjct: 78 EASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQ--RVWTGGGDEETLSKGV 135
Query: 85 RMATERVPLRPNALRVID-GKVVG---NVP---EVHFEPGDETKISILMKGGGSENCTAL 137
LR + +D K V N+P +++ GDE K + KGGGS N T L
Sbjct: 136 YNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYL 195
Query: 138 F-----TLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGI-ASPAEKALILAKKA--- 188
+ LTPG+ LK ++E +++ G CPP + I + AE L K A
Sbjct: 196 YQETKALLTPGK----LKNFLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAH 251
Query: 189 ----LLRKIGERNENEKLAMFEEELLEEINSLGIGPMGLGGKTTALDVKVEVETRHPASY 244
L + E + + E+ELLEE LG+G GGK A D++V RH AS
Sbjct: 252 YYDELPTEGNEHGQAFRDVQLEQELLEEAQKLGLGAQ-FGGKYFAHDIRVIRLPRHGASC 310
Query: 245 IAGVAIQCWAHRRVFLEVKGGRVRIW 270
G+ + C A R + + K R IW
Sbjct: 311 PVGMGVSCSADRNI--KAKINREGIW 334
>sp|P00923|FUMA_ECOLI FUMARATE HYDRATASE CLASS I, AEROBIC (FUMARASE)
>gi|68292|pir||UFECAQ fumarate hydratase (EC 4.2.1.2)
fumA, iron-dependent - Escherichia coli
>gi|41511|emb|CAA25204.1| (X00522) fumarase [Escherichia
coli] >gi|1742662|dbj|BAA15360.1| (D90804) Fumarate
hydratase (EC 4.2.1.2) FumA, iron-dependent [Escherichia
coli] >gi|1742667|dbj|BAA15364.1| (D90805) Fumarate
hydratase (EC 4.2.1.2) FumA, iron-dependent [Escherichia
coli] >gi|1787897|gb|AAC74684.1| (AE000256) fumarase A =
fumarate hydratase Class I; aerobic isozyme [Escherichia
coli K12]
Length = 548
Score = 80.8 bits (196), Expect = 2e-14
Identities = 84/275 (30%), Positives = 119/275 (42%), Gaps = 34/275 (12%)
Query: 22 EAIKDALRREE-SEVAKFNLNMILKAIEIGKREGIPVCQDTGTPIFFVKTRE-------- 72
+ + D LR E SE K+ L+ +I + +P CQDTGT I K +
Sbjct: 68 QQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGD 127
Query: 73 ----INEVYEEIVEGVRMATERVPLRPNALRVIDGKVVGNVPEVHFEPGDETKISILMKG 128
VY +E ++ PL V G + +++ GDE K + KG
Sbjct: 128 EAALARGVYNTYIEDNLRYSQNAPL-DMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKG 186
Query: 129 GGSENCTALF-----TLTPGEGLEGLKRKVIEHVKSCGGKPCPPIIVGIGI-ASPAEKAL 182
GGS N T L+ LTPG+ LK ++E +++ G CPP + I + AE L
Sbjct: 187 GGSANKTYLYQETKALLTPGK----LKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNL 242
Query: 183 ILAKKALLRKIGE--RNENEKLAMF-----EEELLEEINSLGIGPMGLGGKTTALDVKVE 235
K A + E NE F E+ELL E +LG+G GGK A D++V
Sbjct: 243 KTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQ-FGGKYFAHDIRVI 301
Query: 236 VETRHPASYIAGVAIQCWAHRRVFLEVKGGRVRIW 270
RH AS G+ + C A R + + K R IW
Sbjct: 302 RLPRHGASCPVGMGVSCSADRNI--KAKINRQGIW 334
>emb|CAC05889.1| (AL391754) fumarate hydratase class I [Streptomyces coelicolor]
Length = 558
Score = 70.2 bits (169), Expect = 2e-11
Identities = 82/285 (28%), Positives = 123/285 (42%), Gaps = 30/285 (10%)
Query: 7 EAIRMAVTRIPDDVVEAIKDA----LRR-----EESEVAKFNLNMILKAIEIGKREGIPV 57
EA+R D+ ++ A LRR E S KF +LK I +P+
Sbjct: 46 EALRKLAEEAVHDIQHYLRPAHLAQLRRIIDDPEASGNDKFVALDLLKNANIAAAGVLPM 105
Query: 58 CQDTGTPIFFVKTRE--INEVYEE--IVEGVRMATERVPLRPNALRVI----DGKVVGNV 109
CQDTGT I K + + E +E + G+ A + + LR + + + + N+
Sbjct: 106 CQDTGTAIVMGKRGQNVLTEGGDEEALSRGIYDAYKNLNLRYSQMAPLTMWEEKNTGSNL 165
Query: 110 P---EVHFEPGDETKISILMKGGGSENCTALFTLTPGEGLEGLKRKVIEH-VKSCGGKPC 165
P E++ G K + KGGGS N + L+ T E K +E ++S G C
Sbjct: 166 PAQIELYATDGGAYKFLFMAKGGGSANKSFLYQETKAVLNESSMMKFLEEKIRSLGTAAC 225
Query: 166 PPIIVGIGIA-SPAEKALILAKKALLRKIGE-RNENEKLAM------FEEELLEEINSLG 217
PP + I + + AE AL AK A + E E +L EE++ E +G
Sbjct: 226 PPYHLAIVVGGTSAEYALKTAKYASAHYLDEIPAEGSELGHGFRDKELEEKVFELTQKIG 285
Query: 218 IGPMGLGGKTTALDVKVEVETRHPASYIAGVAIQCWAHRRVFLEV 262
IG GGK DV+V RH AS +A+ C A R+ ++
Sbjct: 286 IGAQ-FGGKYFCHDVRVVRLPRHGASCPVAIAVSCSADRQAVAKI 329
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.139 0.401
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99927761
Number of Sequences: 2977
Number of extensions: 4316377
Number of successful extensions: 10684
Number of sequences better than 1.0e-10: 24
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 10611
Number of HSP's gapped (non-prelim): 27
length of query: 271
length of database: 189,106,746
effective HSP length: 55
effective length of query: 216
effective length of database: 156,189,961
effective search space: 33737031576
effective search space used: 33737031576
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 164 (68.3 bits)