BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2049 (trpA) DE:tryptophan synthase, subunit alpha (trpA)
(248 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75162 tryptophan synthase, chain alpha (trpa) PAB2049 - Py... 492 e-138
sp|Q9YGA9|TRPA_PYRKO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|1126925... 374 e-103
sp|P14637|TRPA_METVO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|150073|... 266 2e-70
gi|11499192 tryptophan synthase, subunit alpha (trpA) [Archaeogl... 261 6e-69
sp|O27697|TRPA_METTH TRYPTOPHAN SYNTHASE ALPHA CHAIN 205 6e-52
pir||A69089 tryptophan synthase, subunit alpha - Methanobacteriu... 205 6e-52
sp|P26920|TRPA_METTM TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|99115|p... 203 2e-51
pir||E64429 tryptophan synthase (EC 4.2.1.20) alpha chain - Meth... 200 1e-50
sp|Q60180|TRPA_METJA TRYPTOPHAN SYNTHASE ALPHA CHAIN 200 1e-50
pir||T01088 tryptophan synthase (EC 4.2.1.20) alpha chain T10P11... 188 5e-47
sp|P77960|TRPA_SYNY3 TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436986... 182 4e-45
pir||S59519 tryptophan synthase (EC 4.2.1.20) alpha chain - Arab... 175 5e-43
sp|O67502|TRPA_AQUAE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436992... 175 5e-43
pir||G83640 tryptophan synthase alpha chain PA0035 [imported] - ... 175 6e-43
sp|P07344|TRPA_PSEAE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|151617|... 174 8e-43
sp|Q44604|TRPA_BUCSC TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|769839|... 174 1e-42
sp|P19867|TRPA_BACST TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80228|p... 174 1e-42
pir||B35407 tryptophan synthase (EC 4.2.1.20) alpha chain - Ther... 173 2e-42
pir||TSPSAA tryptophan synthase (EC 4.2.1.20) alpha chain - Pseu... 173 2e-42
sp|P18284|TRPA_HALVO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|99230|p... 172 4e-42
sp|P42390|TRPA_MAIZE TRYPTOPHAN SYNTHASE ALPHA CHAIN PRECURSOR >... 171 7e-42
sp|P16608|TRPA_THETH TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|295402|... 171 9e-42
sp|P11081|TRPA_PSEPU TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|77776|p... 170 2e-41
sp|Q9ZJV0|TRPA_HELPJ TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436989... 167 1e-40
dbj|BAB05383.1| (AP001512) tryptophan synthase (alpha subunit) [... 165 5e-40
sp|P34816|TRPA_PSESY TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|476481|... 164 1e-39
sp|P22095|TRPA_VIBPA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|48491|e... 163 2e-39
pir||E75455 tryptophan synthase, alpha subunit - Deinococcus rad... 162 3e-39
gb|AAB60041.1| (U23495) tryptophan synthase alpha subunit [Esche... 162 5e-39
gb|AAB60056.1| (U23499) tryptophan synthase alpha subunit [Esche... 162 5e-39
gb|AAB60052.1| (U23498) tryptophan synthase alpha subunit [Esche... 161 8e-39
sp|P00928|TRPA_ECOLI TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|68329|p... 161 8e-39
gb|AAA65158.1| (U23493) tryptophan synthase alpha subunit [Esche... 161 8e-39
gb|AAB60046.1| (U23497) tryptophan synthase alpha subunit [Esche... 161 8e-39
gb|AAA65176.1| (U23500) tryptophan synthase alpha subunit [Esche... 161 1e-38
sp|P56141|TRPA_HELPY TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436990... 161 1e-38
pir||B81934 probable tryptophan synthase alpha chain NMA0879 [im... 161 1e-38
pir||D82232 tryptophan synthase, alpha chain VC1169 [imported] -... 160 2e-38
gb|AAA65170.1| (U23496) tryptophan synthase alpha subunit [Esche... 159 2e-38
sp|O68429|TRPA_BUCDN TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|3170098... 159 2e-38
sp|P00929|TRPA_SALTY TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|68330|p... 159 3e-38
pdb|1QOP|A Chain A, Crystal Structure Of Wild-Type Tryptophan Sy... 159 3e-38
gb|AAF14255.1|AF132318_5 (AF132318) alpha subunit of tryptophan ... 159 4e-38
gi|6321412 tryptophan synthetase; Trp5p [Saccharomyces cerevisia... 158 7e-38
pir||D81171 tryptophan synthase, alpha chain NMB0678 [imported] ... 157 9e-38
sp|P00930|TRPA_KLEAE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|43784|e... 157 1e-37
sp|P12291|TRPA_CAUCR TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|279953|... 157 1e-37
gi|11465848 tryptophan synthase alpha subunit [Porphyra purpurea... 157 1e-37
pdb|1A5A|A Chain A, Cryo-Crystallography Of A True Substrate, In... 157 1e-37
emb|CAA79517.1| (Z19090) tryptophan synthase; alpha subunit [Vib... 157 1e-37
sp|P13228|TRP_NEUCR TRYPTOPHAN SYNTHASE >gi|101877|pir||A32959 t... 157 2e-37
pdb|2TSY|A Chain A, Crystal Structures Of Mutant (Betak87t) Tryp... 155 3e-37
sp|Q9X4E8|TRPA_RHOSH TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|4761131... 155 3e-37
sp|P31204|TRPA_ANTSP TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|421736|... 155 4e-37
pir||TSAEAA tryptophan synthase (EC 4.2.1.20) alpha chain - Kleb... 155 6e-37
sp|P07601|TRPA_BACSU TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80434|p... 154 8e-37
sp|O22018|TRPA_CYAME TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|2541878... 154 1e-36
gb|AAF61458.1|AF139661_3 (AF139661) tryptophan synthase alpha su... 153 2e-36
gb|AAD51339.1|AF173835_3 (AF173835) tryptophan synthetase alpha ... 152 3e-36
sp|P06562|TRPA_BRELA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80601|p... 152 5e-36
sp|P17166|TRPA_LACCA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80565|p... 151 7e-36
sp|P57365|TRPA_BUCAI TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|1003895... 149 3e-35
gb|AAF91181.1|AF207903_2 (AF207903) bifunctional tryptophan synt... 148 8e-35
sp|P42389|TRPA_BUCAP TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|625651|... 148 8e-35
sp|O68816|TRPA_STRCO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7481831... 146 2e-34
sp|O13831|TRP_SCHPO TRYPTOPHAN SYNTHASE >gi|7493535|pir||T37946 ... 145 7e-34
pir||D82688 tryptophan synthase alpha chain XF1376 [imported] - ... 144 9e-34
pir||S41995 tryptophan synthase (EC 4.2.1.20) alpha chain - red ... 143 3e-33
sp|P43759|TRPA_HAEIN TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|1075399... 142 3e-33
dbj|BAA04617.1| (D17791) Tryptophan synthase alpha subunit [Cyan... 140 1e-32
sp|P50908|TRPA_THEMA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|4980629... 140 1e-32
sp|P34793|TRPA_CYACA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7484298... 139 2e-32
pir||S59050 tryptophan synthase alpha chain trpA - Thermotoga ma... 138 6e-32
gi|11465459 unknown; tryptophan synthase alpha subunit [Cyanidiu... 138 6e-32
sp|O68906|TRPA_MYCIT TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|3152734... 130 1e-29
sp|P16578|TRP_COPCI TRYPTOPHAN SYNTHASE >gi|83890|pir||JU0401 tr... 129 2e-29
sp|O06130|TRPA_MYCTU TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436996... 129 2e-29
sp|Q01997|TRPA_LACLA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|421429|... 128 6e-29
pir||PN0500 tryptophan synthase (EC 4.2.1.20) alpha chain - Baci... 118 8e-26
gb|AAC60451.1| (S65045) trpA [Bacillus subtilis, K, Peptide Part... 117 1e-25
gb|AAD41125.1|AF157826_2 (AF157826) tryptophan synthase alpha ch... 115 7e-25
gb|AAD41117.1|AF157822_2 (AF157822) tryptophan synthase alpha ch... 114 9e-25
gb|AAD41123.1|AF157825_2 (AF157825) tryptophan synthase alpha ch... 114 9e-25
gb|AAD41109.1|AF157818_2 (AF157818) tryptophan synthase alpha ch... 114 1e-24
gb|AAD41113.1|AF157820_2 (AF157820) tryptophan synthase alpha ch... 114 1e-24
gb|AAD41107.1|AF157817_2 (AF157817) tryptophan synthase alpha ch... 113 2e-24
gb|AAD41115.1|AF157821_2 (AF157821) tryptophan synthase alpha ch... 113 2e-24
gb|AAD41121.1|AF157824_2 (AF157824) tryptophan synthase alpha ch... 113 2e-24
gb|AAD41111.1|AF157819_2 (AF157819) tryptophan synthase alpha ch... 112 4e-24
pir||D81377 tryptophan synthase (EC 4.2.1.20) alpha chain Cj0349... 112 5e-24
gb|AAD41119.1|AF157823_2 (AF157823) tryptophan synthase alpha ch... 110 1e-23
emb|CAA24636.1| (V01343) put. tryptophan synthetase [Saccharomyc... 109 2e-23
gb|AAG18888.1| (AE004991) tryptophan synthase alpha chain; TrpA ... 109 3e-23
gb|AAK41172.1| Tryptophan synthase alpha chain (trpA) [Sulfolobu... 104 1e-21
sp|P50382|TRPA_SULSO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|1004320... 104 1e-21
dbj|BAA96470.1| (AB036428) hypothetical tryptophan synthase alph... 99 6e-20
sp|O84173|TRPA_CHLTR TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436995... 98 1e-19
emb|CAB63649.1| (AJ249639) tryptophan synthase alpha chain [Chla... 90 2e-17
emb|CAB63648.1| (AJ249638) tryptophan synthase alfa chain [Chlam... 90 2e-17
gb|AAD04348.1| (AF084880) mutant tryptophan synthase [Neurospora... 87 2e-16
gb|AAD04350.1| (AF084882) mutant tryptophan synthase [Neurospora... 86 4e-16
gb|AAD04349.1| (AF084881) mutant tryptophan synthase [Neurospora... 85 7e-16
gb|AAD04354.1| (AF084886) mutant tryptophan synthase [Neurospora... 83 2e-15
gb|AAD04358.1| (AF084890) mutant tryptophan synthase [Neurospora... 83 4e-15
sp|Q9Y8T3|TRPA_AERPE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436993... 81 1e-14
gb|AAD04355.1| (AF084887) mutant tryptophan synthase [Neurospora... 79 4e-14
gb|AAD04356.1| (AF084888) mutant tryptophan synthase [Neurospora... 79 6e-14
gb|AAD04357.1| (AF084889) mutant tryptophan synthase [Neurospora... 78 7e-14
>pir||E75162 tryptophan synthase, chain alpha (trpa) PAB2049 - Pyrococcus abyssi
(strain Orsay) >gi|5457890|emb|CAB49380.1| (AJ248284)
tryptophan synthase, subunit alpha (trpA) [Pyrococcus
abyssi]
Length = 248
Score = 492 bits (1252), Expect = e-138
Identities = 248/248 (100%), Positives = 248/248 (100%)
Query: 1 MFRDGSLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALK 60
MFRDGSLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALK
Sbjct: 1 MFRDGSLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALK 60
Query: 61 GGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
GGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM
Sbjct: 61 GGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF
Sbjct: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE
Sbjct: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
Query: 241 KVRELAGL 248
KVRELAGL
Sbjct: 241 KVRELAGL 248
>sp|Q9YGA9|TRPA_PYRKO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|11269250|pir||T43928 tryptophan
synthase (EC 4.2.1.20) alpha chain [imported] -
Pyrococcus kodakaraensis >gi|5545310|dbj|BAA82551.1|
(AB030011) alpha subunit of tryptophan synthase
[Thermococcus kodakaraensis]
Length = 251
Score = 374 bits (949), Expect = e-103
Identities = 178/247 (72%), Positives = 215/247 (86%), Gaps = 1/247 (0%)
Query: 1 MFRDGSLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALK 60
MF GSLIPYLTAGDPS + TL FLLA+EE++G IELGIPFSDP+ADGKTIQ+SH+RAL+
Sbjct: 1 MFEKGSLIPYLTAGDPSVEKTLEFLLAVEEFAGLIELGIPFSDPMADGKTIQESHYRALR 60
Query: 61 GGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
GFKL+ F I+REFR+HS P++LMTYYNPVFR G+++F+G+AK SG DG+L+VDLPV
Sbjct: 61 NGFKLDDTFRILREFRRHSSTPVILMTYYNPVFRTGVKKFLGEAKASGADGILVVDLPVS 120
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
HA EFL+ A+EEG+KTVFLAAPNTPDERL+EIDKASTGFVYLISLYGTTGARD++PETAF
Sbjct: 121 HAGEFLDAAKEEGLKTVFLAAPNTPDERLREIDKASTGFVYLISLYGTTGARDRLPETAF 180
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
++RA++IC NK+AVGFGVS+RE VE LL AGA+GVVVGSALI +I+ EN E+LR
Sbjct: 181 EFVRRARKICNNKLAVGFGVSRREQVEELLKAGADGVVVGSALIELIS-RSENPVEELRR 239
Query: 241 KVRELAG 247
KV EL+G
Sbjct: 240 KVAELSG 246
>sp|P14637|TRPA_METVO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|150073|gb|AAA72855.1| (M35130)
tryptophan synthase A [Methanococcus voltae]
Length = 284
Score = 266 bits (673), Expect = 2e-70
Identities = 124/243 (51%), Positives = 182/243 (74%), Gaps = 3/243 (1%)
Query: 7 LIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
L+ +L +GDP+ +ATL+F+ A++EY G IELGIPFSDPIADG TIQ+++ R+L G+K+
Sbjct: 26 LVSFLVSGDPNIEATLKFMNALDEYCGVIELGIPFSDPIADGSTIQEANVRSLSNGYKIH 85
Query: 67 HAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEFL 126
+F+++REFRK SD P+VLMTYYNP+++ G+ F+ +AK++G +G++IVDLP+ A ++
Sbjct: 86 QSFDVLREFRKFSDTPVVLMTYYNPIYKRGIENFVIQAKEAGANGLIIVDLPLDEAEQYR 145
Query: 127 EVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKRA 186
+ ++ + TVFL APNTPDERL D+AST F+Y+IS +G TGAR + F + RA
Sbjct: 146 AICKKHDMGTVFLVAPNTPDERLMYSDEASTLFLYVISTFGITGARGSFEKMTFEFIARA 205
Query: 187 KRIC-KNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEE--KLREKVR 243
K +C KNK+ VGFG+S EH E ++ GA+GV+VGSA ++II E+G++ E KL+E R
Sbjct: 206 KNLCDKNKLYVGFGISNGEHAEKIIENGADGVIVGSAFVDIIKEYGDSNETIYKLKELAR 265
Query: 244 ELA 246
EL+
Sbjct: 266 ELS 268
>gi|11499192 tryptophan synthase, subunit alpha (trpA) [Archaeoglobus fulgidus]
>gi|3334388|sp|O28673|TRPA_ARCFU TRYPTOPHAN SYNTHASE
ALPHA CHAIN >gi|7436991|pir||F69449 tryptophan synthase,
subunit alpha (trpA) homolog - Archaeoglobus fulgidus
>gi|2648961|gb|AAB89650.1| (AE000992) tryptophan
synthase, subunit alpha (trpA) [Archaeoglobus fulgidus]
Length = 248
Score = 261 bits (660), Expect = 6e-69
Identities = 133/243 (54%), Positives = 173/243 (70%), Gaps = 3/243 (1%)
Query: 4 DGSLIPYLTAGDPSAKATLRFLL-AIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGG 62
D SLI + TA P+A+ T+ F+L A E + IELG+PFSDP+ADGKTIQ+S+ RAL+
Sbjct: 3 DRSLIVFFTACYPTAEKTVEFMLTAAESGADVIELGVPFSDPVADGKTIQESYVRALRN- 61
Query: 63 FKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHA 122
F++E F I + FR SD P+VLM+YYNP++R G+ F+ KA SG+D ML+VDLP A
Sbjct: 62 FRVERVFEIAKAFRAESDKPLVLMSYYNPIYRRGVESFVEKAYSSGIDAMLVVDLPYDEA 121
Query: 123 SEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNL 182
+F+EV G+K VFLAAPNTP++RL+ +D+ S FVYL+S YG TG RD+I AF
Sbjct: 122 GDFVEVCSRTGMKNVFLAAPNTPEDRLRAMDELS-AFVYLVSTYGVTGERDRISPLAFEA 180
Query: 183 LKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKV 242
LKRAK IC+ VAVGFGVSK +HV L++AGA+GVVVGSA + +I E GE A E +R
Sbjct: 181 LKRAKGICRKPVAVGFGVSKADHVRQLISAGADGVVVGSAFVRLINEKGERATEDIRALT 240
Query: 243 REL 245
L
Sbjct: 241 ENL 243
>sp|O27697|TRPA_METTH TRYPTOPHAN SYNTHASE ALPHA CHAIN
Length = 270
Score = 205 bits (515), Expect = 6e-52
Identities = 106/251 (42%), Positives = 162/251 (64%), Gaps = 5/251 (1%)
Query: 1 MFR--DG-SLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHF 56
MFR DG + +P++ AGDP + +L + ++ + A+E+G PFSDPIADG ++Q +
Sbjct: 12 MFRRADGCAFVPFVVAGDPDMETSLEIIRTLVDAGADALEVGFPFSDPIADGTSVQGADL 71
Query: 57 RALKGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVD 116
RAL+ G E F ++ R+ + +PI L+ YYN ++R+G+ EF +A ++GV G+L D
Sbjct: 72 RALRAGMTTEKCFQLIERVREFTSIPIGLLVYYNLIYRMGVDEFYRRAAEAGVTGILAAD 131
Query: 117 LPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIP 176
LP AS+ L A + I +F+ AP T ERLK I + S+GF YL+S+ G TGAR ++
Sbjct: 132 LPPEEASDALRAAEKYDIDQIFIVAPTTGSERLKRISEVSSGFHYLVSVMGVTGARSRVE 191
Query: 177 ETAFNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEE 236
+ L+KR K V VGFGVS+ EHV ML +AGA+GV+VGSA+I++I+ + + E
Sbjct: 192 DATIELIKRVKAEGSLPVMVGFGVSRPEHVRMLRDAGADGVIVGSAIIDVISGNLGDREL 251
Query: 237 KLREKVRELAG 247
L +++ E+AG
Sbjct: 252 ML-QRIHEMAG 261
>pir||A69089 tryptophan synthase, subunit alpha - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622788|gb|AAB86132.1| (AE000924) tryptophan
synthase, subunit alpha [Methanobacterium
thermoautotrophicum]
Length = 273
Score = 205 bits (515), Expect = 6e-52
Identities = 106/251 (42%), Positives = 162/251 (64%), Gaps = 5/251 (1%)
Query: 1 MFR--DG-SLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHF 56
MFR DG + +P++ AGDP + +L + ++ + A+E+G PFSDPIADG ++Q +
Sbjct: 15 MFRRADGCAFVPFVVAGDPDMETSLEIIRTLVDAGADALEVGFPFSDPIADGTSVQGADL 74
Query: 57 RALKGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVD 116
RAL+ G E F ++ R+ + +PI L+ YYN ++R+G+ EF +A ++GV G+L D
Sbjct: 75 RALRAGMTTEKCFQLIERVREFTSIPIGLLVYYNLIYRMGVDEFYRRAAEAGVTGILAAD 134
Query: 117 LPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIP 176
LP AS+ L A + I +F+ AP T ERLK I + S+GF YL+S+ G TGAR ++
Sbjct: 135 LPPEEASDALRAAEKYDIDQIFIVAPTTGSERLKRISEVSSGFHYLVSVMGVTGARSRVE 194
Query: 177 ETAFNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEE 236
+ L+KR K V VGFGVS+ EHV ML +AGA+GV+VGSA+I++I+ + + E
Sbjct: 195 DATIELIKRVKAEGSLPVMVGFGVSRPEHVRMLRDAGADGVIVGSAIIDVISGNLGDREL 254
Query: 237 KLREKVRELAG 247
L +++ E+AG
Sbjct: 255 ML-QRIHEMAG 264
>sp|P26920|TRPA_METTM TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|99115|pir||D40362 trpA protein
- Methanobacterium thermoautotrophicum (strain Marburg)
>gi|149750|gb|AAA73033.1| (M65060) tryptophan synthase
alpha-subunit [Methanobacterium thermoautotrophicum]
Length = 265
Score = 203 bits (511), Expect = 2e-51
Identities = 96/236 (40%), Positives = 154/236 (64%), Gaps = 1/236 (0%)
Query: 6 SLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+ +P++ AGDP + ++ + ++ + A+E+G PFSDPIADG ++Q++ RAL G
Sbjct: 14 AFVPFVVAGDPDMETSVEIIRTLVDAGADALEIGFPFSDPIADGTSVQEADLRALNSGMT 73
Query: 65 LEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
+ F ++ R+ + VP+ L+ YYN ++R+G EF +A ++GV G+L DLP A +
Sbjct: 74 TDDCFRLIERIREFTSVPVGLLVYYNLIYRMGADEFYRRAAEAGVTGILAADLPPEEAGD 133
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
L A GI +F+ AP T ++RLK I + S+GF YL+S+ G TGAR ++ E+ L++
Sbjct: 134 ALRAAERYGIDQIFIVAPTTDNQRLKMISEVSSGFHYLVSVMGVTGARSRVEESTLELIE 193
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
R K V VGFGVS+ EHV ML +AGA+GV+VGSA+I++I+++ ++ E L+E
Sbjct: 194 RVKGAGSLPVMVGFGVSQPEHVRMLADAGADGVIVGSAIIDLISKNLDDRERMLQE 249
>pir||E64429 tryptophan synthase (EC 4.2.1.20) alpha chain - Methanococcus
jannaschii >gi|1591691|gb|AAB99041.1| (U67546)
tryptophan synthase alpha subunit (trpA) [Methanococcus
jannaschii]
Length = 284
Score = 200 bits (504), Expect = 1e-50
Identities = 100/238 (42%), Positives = 153/238 (64%), Gaps = 3/238 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
+ + + GDP+ + + + L I +++ +E+GIPFSDP+ADG TIQ++ RAL G
Sbjct: 22 AFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDPVADGITIQKADVRALNSGMNP 81
Query: 66 EHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
AF + ++ K +VP V +TYYN +F++G EF+ K K++GV G+++ DLP+ A
Sbjct: 82 LKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKKCKEAGVSGIIVPDLPIEEADS 141
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
++ G+ +FL AP TPDERLK+I + +GFVY++S+ G TGAR+K+ E L+K
Sbjct: 142 LYNYCKKYGVDLIFLVAPTTPDERLKKILEKCSGFVYVVSVTGITGAREKVAEETKELIK 201
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEH-GENAEEKLREK 241
R K+ K VGFG+SKREHVE + A+G +VGSA++ I+ +H EN + K EK
Sbjct: 202 RVKKFSKIPACVGFGISKREHVEEITEI-ADGAIVGSAIVKIVEKHLDENGQIKDEEK 258
>sp|Q60180|TRPA_METJA TRYPTOPHAN SYNTHASE ALPHA CHAIN
Length = 281
Score = 200 bits (504), Expect = 1e-50
Identities = 100/238 (42%), Positives = 153/238 (64%), Gaps = 3/238 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
+ + + GDP+ + + + L I +++ +E+GIPFSDP+ADG TIQ++ RAL G
Sbjct: 19 AFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDPVADGITIQKADVRALNSGMNP 78
Query: 66 EHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
AF + ++ K +VP V +TYYN +F++G EF+ K K++GV G+++ DLP+ A
Sbjct: 79 LKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKKCKEAGVSGIIVPDLPIEEADS 138
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
++ G+ +FL AP TPDERLK+I + +GFVY++S+ G TGAR+K+ E L+K
Sbjct: 139 LYNYCKKYGVDLIFLVAPTTPDERLKKILEKCSGFVYVVSVTGITGAREKVAEETKELIK 198
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEH-GENAEEKLREK 241
R K+ K VGFG+SKREHVE + A+G +VGSA++ I+ +H EN + K EK
Sbjct: 199 RVKKFSKIPACVGFGISKREHVEEITEI-ADGAIVGSAIVKIVEKHLDENGQIKDEEK 255
>pir||T01088 tryptophan synthase (EC 4.2.1.20) alpha chain T10P11.11 -
Arabidopsis thaliana >gi|3892048|gb|AAC78257.1|AAC78257
(AC002330) putative tryptophan synthase alpha 1-like
protein [Arabidopsis thaliana]
>gi|7269021|emb|CAB80754.1| (AL161494) tryptophan
synthase alpha 1-like protein [Arabidopsis thaliana]
Length = 275
Score = 188 bits (473), Expect = 5e-47
Identities = 97/236 (41%), Positives = 143/236 (60%), Gaps = 2/236 (0%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEY-SGAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LIPY+TAGDP T + L ++ S IELG+P+SDP+ADG IQ + R+L G
Sbjct: 27 ALIPYITAGDPDLSTTAKALKVLDSCGSDIIELGVPYSDPLADGPAIQAAARRSLLKGTN 86
Query: 65 LEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
++++E PI L TYYNP+ R G+ ++ K++GV G+L+ D+P+
Sbjct: 87 FNSIISMLKEVIPQLSCPIALFTYYNPILRRGVENYMTVIKNAGVHGLLVPDVPLEETET 146
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
AR+ I+ V L P TP ER+ I +AS GF+YL+S G TG R+ + E +LL+
Sbjct: 147 LRNEARKHQIELVLLTTPTTPKERMNAIVEASEGFIYLVSSVGVTGTRESVNEKVQSLLQ 206
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+ K VAVGFG+SK EHV+ + GA+GV+VGSA++ I+ E E+ E+ L+E
Sbjct: 207 QIKEATSKPVAVGFGISKPEHVKQVAEWGADGVIVGSAMVKILGE-SESPEQGLKE 261
>sp|P77960|TRPA_SYNY3 TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436986|pir||S74721 tryptophan
synthase (EC 4.2.1.20) alpha chain - Synechocystis sp.
(strain PCC 6803) >gi|1651946|dbj|BAA16872.1| (D90901)
tryptophan synthase alpha chain [Synechocystis sp.]
Length = 264
Score = 182 bits (457), Expect = 4e-45
Identities = 97/226 (42%), Positives = 138/226 (60%), Gaps = 3/226 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGF 63
+LIP+LTAGDP AT L I + +GA IELG+P+SDP+ADG IQ + RAL+ G
Sbjct: 18 ALIPFLTAGDPDL-ATTAEALRILDRAGADLIELGVPYSDPLADGPVIQAAATRALQKGV 76
Query: 64 KLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
KL+ IVRE + PI+L TYYNP+F G+ F+ K K +GV G+++ DLP+ +
Sbjct: 77 KLDDVLAIVREVHQDIAAPIILFTYYNPIFYQGVEVFLDKIKAAGVKGLVVPDLPLEESD 136
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
LE E G++ + L AP + ER I K S GFVYL+S+ G TG R ++ LL
Sbjct: 137 RLLEATAERGLELILLVAPTSSPERQTAIAKKSQGFVYLVSVTGVTGVRTEVGSRVEALL 196
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE 229
+++ + VGFG+S+ E E + GA+ V+VGSA++ +AE
Sbjct: 197 AGMRQVTDKPIGVGFGISQPEQAEQVKAWGADAVIVGSAMVKRLAE 242
>pir||S59519 tryptophan synthase (EC 4.2.1.20) alpha chain - Arabidopsis
thaliana >gi|619753|gb|AAC49117.1| (U18993) tryptophan
synthase alpha chain [Arabidopsis thaliana]
>gi|7258367|emb|CAB77584.1| (AL138656) tryptophan
synthase alpha chain [Arabidopsis thaliana]
>gi|1585768|prf||2201482A Trp synthase:SUBUNIT=alpha
[Arabidopsis thaliana]
Length = 312
Score = 175 bits (439), Expect = 5e-43
Identities = 93/236 (39%), Positives = 137/236 (57%), Gaps = 2/236 (0%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEY-SGAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+ IPY+TAGDP T L ++ S IELG+P+SDP+ADG IQ + R+L+ G
Sbjct: 65 AFIPYITAGDPDLSTTAEALKVLDACGSDIIELGVPYSDPLADGPVIQAAATRSLERGTN 124
Query: 65 LEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
L+ ++ + PI L TYYNP+ + GL +F+ + GV G+++ D+P+
Sbjct: 125 LDSILEMLDKVVPQISCPISLFTYYNPILKRGLGKFMSSIRAVGVQGLVVPDVPLEETEM 184
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
+ A I+ V L P TP ER+K I AS GF+YL+S G TGAR + +LLK
Sbjct: 185 LRKEALNNDIELVLLTTPTTPTERMKLIVDASEGFIYLVSSIGVTGARSSVSGKVQSLLK 244
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
K VAVGFG+SK EHV+ + GA+GV+VGSA++ ++ + ++ E L+E
Sbjct: 245 DIKEATDKPVAVGFGISKPEHVKQIAGWGADGVIVGSAMVKLLGD-AKSPTEGLKE 299
>sp|O67502|TRPA_AQUAE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436992|pir||D70434 tryptophan
synthase alpha subunit - Aquifex aeolicus
>gi|2983908|gb|AAC07458.1| (AE000745) tryptophan
synthase alpha subunit [Aquifex aeolicus]
Length = 262
Score = 175 bits (439), Expect = 5e-43
Identities = 97/246 (39%), Positives = 147/246 (59%), Gaps = 7/246 (2%)
Query: 3 RDGSLIPYLTAGDPSAKATLR-FLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
R+ +L+ YL G P + +L+ F ++ + +E+G PFSDP+ADG TIQ +H ALK
Sbjct: 15 REKALVSYLMVGYPDYETSLKAFKEVLKNGTDILEIGFPFSDPVADGPTIQVAHEVALKN 74
Query: 62 GFKLEHAFNIVREFRKH-SDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G + E + RK D+P +LMTYYNP+FR+GL +F +++ G+DG ++ DLP
Sbjct: 75 GIRFEDVLELSETLRKEFPDIPFLLMTYYNPIFRIGLEKFCRLSREKGIDGFIVPDLPPE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIP-ETA 179
A E V ++ + V L AP + +R+K I +A+ Y +S+ GTTGAR+K+P E
Sbjct: 135 EAEELKAVMKKYVLSFVPLGAPTSTRKRIKLICEAADEMTYFVSVTGTTGAREKLPYERI 194
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLR 239
++ + +C V VGFGVSK+EH + + A+GVVVGSAL+ + G+ E L
Sbjct: 195 KKKVEEYRELCDKPVVVGFGVSKKEHARE-IGSFADGVVVGSALVKLA---GQKKIEDLG 250
Query: 240 EKVREL 245
V+EL
Sbjct: 251 NLVKEL 256
>pir||G83640 tryptophan synthase alpha chain PA0035 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9945856|gb|AAG03425.1|AE004443_3 (AE004443)
tryptophan synthase alpha chain [Pseudomonas aeruginosa]
Length = 268
Score = 175 bits (438), Expect = 6e-43
Identities = 95/232 (40%), Positives = 138/232 (58%), Gaps = 3/232 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ ++TAGDP ++L L + IELG+PF+DP+ADG IQ ++ RAL GG
Sbjct: 18 ALVTFVTAGDPDYASSLEILKGLPAAGADVIELGMPFTDPMADGPAIQLANIRALDGGQT 77
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +VREFR S+ P+VLM Y+NP+ G+ FI +AK+ GVDG+++VDLP H
Sbjct: 78 LARTLQMVREFRSGDSETPLVLMGYFNPIHHYGVERFIAEAKEVGVDGLIVVDLPPEHNE 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
+ A+ GI + L P T D+RL + + S+GFVY +S+ G TGA E +
Sbjct: 138 DLCHPAQAAGIDFIRLTTPTTGDQRLPTVLEGSSGFVYYVSVAGVTGANAATLEHVEEAV 197
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAE 235
R +R + +GFG+ EH + A+GVVVGSALI+ IA+ +NA+
Sbjct: 198 ARLRRHTDLPIGIGFGIRSAEHAAAVARL-ADGVVVGSALIDRIAKARDNAQ 248
>sp|P07344|TRPA_PSEAE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|151617|gb|AAA88463.1| (M15826)
tryptophan synthase alpha subunit [Pseudomonas
aeruginosa]
Length = 268
Score = 174 bits (437), Expect = 8e-43
Identities = 94/232 (40%), Positives = 139/232 (59%), Gaps = 3/232 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ ++TAGDP ++L L + IELG+PF+DP+ADG IQ ++ RAL+GG
Sbjct: 18 ALVTFVTAGDPDYASSLEILKGLPGAGADVIELGMPFTDPMADGPAIQLANIRALEGGQT 77
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +VREFR S+ P+VLM Y+NP+ G+ FI +AK+ GVDG+++VDLP H
Sbjct: 78 LARTLQMVREFRSGDSETPLVLMGYFNPIHHYGVERFIAEAKEVGVDGLIVVDLPPEHNE 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
+ A+ G+ + L P T D+RL + + S+GFVY +S+ G TGA E +
Sbjct: 138 DLCHPAQAAGLDFIRLTTPTTGDQRLPTVLEGSSGFVYYVSVAGVTGANAATLEHVEEAV 197
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAE 235
R +R + +GFG+ EH + A+GVVVGSALI+ IA+ +NA+
Sbjct: 198 ARLRRHTDLPIGIGFGIRSAEHAAAVARL-ADGVVVGSALIDRIAKARDNAQ 248
>sp|Q44604|TRPA_BUCSC TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|769839|gb|AAA92797.1| (U09185)
tryptophan synthase alpha subunit [Buchnera aphidicola]
Length = 269
Score = 174 bits (436), Expect = 1e-42
Identities = 87/247 (35%), Positives = 151/247 (60%), Gaps = 3/247 (1%)
Query: 2 FRDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALK 60
F+ G IP++ GDPS + +L+ + + IE + +ELG PFSDP++DGKTIQ++H RA
Sbjct: 14 FKKGCFIPFIILGDPSIEMSLKIIDVLIENGADGLELGFPFSDPLSDGKTIQKAHLRAFS 73
Query: 61 GGFKLEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
+ F ++++ RK ++ +PI L+ Y N +F+ G+ F K + G+D +LI DLPV
Sbjct: 74 SKMNIYLCFEMLQKIRKKYNTIPIGLLLYANLIFKFGINNFYLKCFNVGIDSVLIADLPV 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
+++F + A I +VF+ + +K+I STG++YL+S G TG KI +
Sbjct: 134 EESNDFRKCAIANNISSVFVCPHDAKKNVIKKISLYSTGYIYLLSRSGVTGTDKKIIVPS 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLR 239
NL+K K+I + + GFG+S + ++ ++ +G +GV+ GS +I +I H +N E+K+
Sbjct: 194 LNLIKNLKKITEKLLIQGFGISNSKQIQKIILSGISGVICGSVIIKLIENHFQN-EKKML 252
Query: 240 EKVRELA 246
+ ++ L+
Sbjct: 253 KNIKRLS 259
>sp|P19867|TRPA_BACST TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80228|pir||JT0525 tryptophan
synthase (EC 4.2.1.20) alpha chain - Bacillus
stearothermophilus >gi|216355|dbj|BAA00428.1| (D00539)
tryptophan synthase alpha-subunit [Bacillus
stearothermophilus] >gi|226586|prf||1603216B Trp
synthase [Bacillus stearothermophilus]
Length = 269
Score = 174 bits (436), Expect = 1e-42
Identities = 99/244 (40%), Positives = 146/244 (59%), Gaps = 8/244 (3%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
IP++ AGDPS + T+ LA+EE +GA +ELG+P+SDP+ADG TIQ++ RAL G L
Sbjct: 12 IPFIVAGDPSPEVTVDLALALEE-AGADLLELGVPYSDPLADGPTIQRAAARALAGNMTL 70
Query: 66 EHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
A ++V E RK +PI+L TYYNPV ++G F A+++G +G+LI DLP +
Sbjct: 71 PKAIHLVAEMRKKGVTIPIILFTYYNPVLQLGEESFFALARENGANGVLIPDLPFEESGP 130
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
E+ + + L AP T +R++ I + GF+Y +S G TG R+ +PE+ + +
Sbjct: 131 LRELGERFDLPLISLVAP-TSKQRIERIASVAQGFLYCVSSLGVTGMRETLPESLGDFVS 189
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGEN--AEEKLREKV 242
KR + VAVGFG+S E V ML +GVV+GSAL+ + + GE A E+ +
Sbjct: 190 EVKRHSRVPVAVGFGISTPEQVAMLKEV-CDGVVIGSALVQKVEQLGERLLAPEEKEAAI 248
Query: 243 RELA 246
E A
Sbjct: 249 AEFA 252
>pir||B35407 tryptophan synthase (EC 4.2.1.20) alpha chain - Thermus aquaticus
Length = 271
Score = 173 bits (434), Expect = 2e-42
Identities = 95/235 (40%), Positives = 143/235 (60%), Gaps = 3/235 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
+LIPYLTAG PS + L+ + + Y+ +E+G+P+SDP+ DG IQ++ AL+ G +
Sbjct: 17 ALIPYLTAGFPSREGFLQAVEEVLPYADLLEIGLPYSDPLGDGPVIQRASELALRKGMSV 76
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
+ A +VRE R ++ P+ LMTY NPV G F G K +G G+++ DLP
Sbjct: 77 QGALELVREVRALTEKPLFLMTYLNPVLAWGPERFFGLFKQAGATGVILPDLPPDEDPGL 136
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKR 185
+ +A+E G++TVFL AP + D R+ + + +TGFVY +S+ G TG R+++PE +L++R
Sbjct: 137 VRLAQEIGLETVFLLAPTSTDARIATVVRHATGFVYAVSVTGVTGMRERLPEEVKDLVRR 196
Query: 186 AKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
K VAVGFGVS + A A+GVVVGSAL+ + E G + L+E
Sbjct: 197 IKARTALPVAVGFGVSGKATAAQA--AVADGVVVGSALVRAL-EEGRSLAPLLQE 248
>pir||TSPSAA tryptophan synthase (EC 4.2.1.20) alpha chain - Pseudomonas
aeruginosa
Length = 267
Score = 173 bits (433), Expect = 2e-42
Identities = 94/232 (40%), Positives = 139/232 (59%), Gaps = 4/232 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ ++TAGDP ++L L + IELG+PF+DP+ADG IQ ++ RAL+GG
Sbjct: 18 ALVTFVTAGDPDYASSLEILKGLPGAGADVIELGMPFTDPMADGPAIQLANIRALEGGQT 77
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +VREFR S+ P+VLM Y+NP+ G+ FI +AK+ GVDG+++VDLP H
Sbjct: 78 LARTLQMVREFRSGDSETPLVLMGYFNPIHHYGVERFIAEAKEVGVDGLIVVDLPPEHNE 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
+ A+ G+ + L P T D+RL + + S+GFVY +S+ G TGA E +
Sbjct: 138 DLCHPAQAAGLDFIRLTTPTTGDQRLPTVLEGSSGFVYYVSVAGVTGANAATLEHVEEAV 197
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAE 235
R +R + +GFG+ EH + A+GVVVGSALI+ IA+ +NA+
Sbjct: 198 ARLRRHTDLPIGIGFGIRSAEHAAVA--RLADGVVVGSALIDRIAKARDNAQ 247
>sp|P18284|TRPA_HALVO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|99230|pir||A36044 tryptophan
synthase (EC 4.2.1.20) alpha chain [validated] -
Haloferax volcanii (strain WFD11)
>gi|149044|gb|AAA72864.1| (M36177) tryptophan synthetase
A (trpA) [Haloferax volcanii]
Length = 277
Score = 172 bits (431), Expect = 4e-42
Identities = 99/253 (39%), Positives = 151/253 (59%), Gaps = 11/253 (4%)
Query: 2 FRDG-SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRAL 59
F DG + +PYL AGDP +++L ++ A+E IELG+PFS+PIA+G TIQ + R+L
Sbjct: 7 FSDGPAFVPYLAAGDPDYESSLEYVEALERGGADVIELGLPFSEPIAEGPTIQNAVVRSL 66
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVG----LREFIGKAKDSGVDGMLIV 115
+GG F V + VP+V M YYN ++R G R F+ KA + G++G ++
Sbjct: 67 EGGMTPTRFFEFVEDL--DVSVPLVCMRYYNLIYRYGDEPGPRPFVEKAAEVGIEGFVVP 124
Query: 116 DLPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKI 175
DLP A E E G+ VF+ AP T ERL I + +G+VY+ + GTTGA+ +
Sbjct: 125 DLPAEEAGPLREACDEFGLDLVFIVAPTTRGERLDRIMEQVSGYVYVQARLGTTGAQSSV 184
Query: 176 PETAFNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAE 235
+ + L+R K AVGFG+S +H E ++ +GA+G++VGSAL++I+AE EN +
Sbjct: 185 SDQTDSSLERLTDYDVPK-AVGFGISDGDHAERIVASGADGIIVGSALVDIVAEGHENGD 243
Query: 236 --EKLREKVRELA 246
E + +++ LA
Sbjct: 244 DAETVADRLETLA 256
>sp|P42390|TRPA_MAIZE TRYPTOPHAN SYNTHASE ALPHA CHAIN PRECURSOR >gi|1362207|pir||S56665
tryptophan synthase (EC 4.2.1.20) alpha chain - maize
>gi|440171|emb|CAA54131.1| (X76713) tryptophan synthase,
alpha subunit [Zea mays]
Length = 346
Score = 171 bits (429), Expect = 7e-42
Identities = 88/225 (39%), Positives = 130/225 (57%), Gaps = 5/225 (2%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+ IPY+TAGDP T L ++ IELG+P SDP DG IQ S RAL G
Sbjct: 103 AFIPYITAGDPDLATTAEALRLLDGCGADVIELGVPCSDPYIDGPIIQASVARALASGTT 162
Query: 65 LEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
++ ++RE P+VL++YY P+ L E K++GV G+++ DLP + A
Sbjct: 163 MDAVLEMLREVTPELSCPVVLLSYYKPIMSRSLAEM----KEAGVHGLIVPDLPYVAAHS 218
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
A+ ++ V L P P++R+KEI KAS GFVYL+S+ G TG R + +L++
Sbjct: 219 LWSEAKNNNLELVLLTTPAIPEDRMKEITKASEGFVYLVSVNGVTGPRANVNPRVESLIQ 278
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE 229
K++ VAVGFG+SK EHV+ + GA+GV++GSA++ + E
Sbjct: 279 EVKKVTNKPVAVGFGISKPEHVKQIAQWGADGVIIGSAMVRQLGE 323
>sp|P16608|TRPA_THETH TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|295402|gb|AAA27509.1| (M32108)
tryptophan synthetase A (gtg start codon; EC 4.2.1.20)
[Thermus thermophilus]
Length = 271
Score = 171 bits (428), Expect = 9e-42
Identities = 94/235 (40%), Positives = 142/235 (60%), Gaps = 3/235 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
+LIPYLTAG PS + L+ + + Y+ +E+G+P+SDP+ DG IQ++ AL+ G +
Sbjct: 17 ALIPYLTAGFPSREGFLQAVEEVLPYADLLEIGLPYSDPLGDGPVIQRASELALRKGMSV 76
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
+ A +VRE R ++ P+ LMTY NPV G F G K +G G+++ DLP
Sbjct: 77 QGALELVREVRALTEKPLFLMTYLNPVLAWGPERFFGLFKQAGATGVILPDLPPDEDPGL 136
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKR 185
+ +A+E G++TVFL AP + D R+ + + +TGF Y +S+ G TG R+++PE +L++R
Sbjct: 137 VRLAQEIGLETVFLLAPTSTDARIATVVRHATGFSYAVSVTGVTGMRERLPEEVKDLVRR 196
Query: 186 AKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
K VAVGFGVS + A A+GVVVGSAL+ + E G + L+E
Sbjct: 197 IKARTALPVAVGFGVSGKATAAQA--AVADGVVVGSALVRAL-EEGRSLAPLLQE 248
>sp|P11081|TRPA_PSEPU TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|77776|pir||A30768 tryptophan
synthase (EC 4.2.1.20) alpha chain - Pseudomonas putida
>gi|45750|emb|CAA31662.1| (X13299) trpA gene product (AA
1 - 269) [Pseudomonas putida]
Length = 269
Score = 170 bits (426), Expect = 2e-41
Identities = 95/226 (42%), Positives = 135/226 (59%), Gaps = 3/226 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ ++TAGDP A+L+ L + IELG+PF+DP+ADG IQ + RAL+ G
Sbjct: 18 ALVTFVTAGDPGYDASLQILKGLPAAGADVIELGMPFTDPMADGVAIQLATLRALEAGQT 77
Query: 65 LEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +VREFR + PIVLM YYNP+ R G+ +F+ +AK +GVDG++IVDLP H +
Sbjct: 78 LAKTLQMVREFRVDNHTTPIVLMGYYNPIHRFGVEKFVAEAKQAGVDGLIIVDLPPEHDA 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
E A+ GI + L P T D RL + + S+GFVY +S+ G TGA E +
Sbjct: 138 ELATPAQAAGIDFIRLTTPTTDDARLPRVLERSSGFVYYVSVAGVTGAGSATTEHVTEAI 197
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE 229
R +R ++VGFG+ E + A+GVVVGSAL++ IA+
Sbjct: 198 ARLRRHTDLPISVGFGIRTPEQAANIARL-ADGVVVGSALVDKIAQ 242
>sp|Q9ZJV0|TRPA_HELPJ TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436989|pir||B71836 tryptophan
synthase alpha chain - Helicobacter pylori (strain J99)
>gi|4155802|gb|AAD06777.1| (AE001546) TRYPTOPHAN
SYNTHASE ALPHA CHAIN [Helicobacter pylori J99]
Length = 262
Score = 167 bits (418), Expect = 1e-40
Identities = 90/245 (36%), Positives = 148/245 (59%), Gaps = 6/245 (2%)
Query: 6 SLIPYLTAGDPSAKATLRFL--LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGF 63
+ IP++T GDP+ + + + L I S A+ELG+ FSDP+ADG TIQ SH RALK
Sbjct: 17 AFIPFVTLGDPNYELSFEIVKTLIISGVS-ALELGLAFSDPVADGTTIQASHLRALKHAS 75
Query: 64 KLEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHA 122
++ F ++++ R ++ D+PI L+ Y N +F G+ F +AK+ GVD +LI D+P++
Sbjct: 76 MAKN-FQLLKKIRGYNHDIPIGLLAYANLIFSYGVDGFYAQAKECGVDSVLIADMPLIEK 134
Query: 123 SEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNL 182
++ A++ IK +F+A+PN + L+++ S G++Y ++ G TGA + A +
Sbjct: 135 ELVIKSAQKHQIKQIFIASPNASSKDLEQVATHSQGYIYTLARSGVTGASHTLENDASAI 194
Query: 183 LKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKV 242
+K K +GFG+SK+EH+ GA+GV+ GSAL+ II E+ N E+ + EK+
Sbjct: 195 IKTLKTFSSTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIEENLNN-EDAMLEKI 253
Query: 243 RELAG 247
+ G
Sbjct: 254 KGFVG 258
>dbj|BAB05383.1| (AP001512) tryptophan synthase (alpha subunit) [Bacillus
halodurans]
Length = 265
Score = 165 bits (413), Expect = 5e-40
Identities = 92/237 (38%), Positives = 138/237 (57%), Gaps = 6/237 (2%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEYSGAI-ELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
IP++TAGDP+ + T+ L +EE I ELGIP+SDP+ADG IQ + RALK LE
Sbjct: 16 IPFITAGDPNPEVTIELALTLEEAGADILELGIPYSDPLADGPVIQAASKRALKHEMTLE 75
Query: 67 HAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
A ++V + R +P+++ TY NP+ + G +F+ +A D VDG+L+ D+P
Sbjct: 76 KALSLVPKMRAQGLTIPVIVFTYVNPLLQYGEEQFVKRAADYQVDGILVPDMPFEEGEVL 135
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKR 185
+E + + L AP T ++R+++I + GFVY +S G TG RD++ L+
Sbjct: 136 QAACQEHNLSLISLVAP-TSNKRIEKIAARAQGFVYCVSSLGVTGLRDELDPRVHEFLQT 194
Query: 186 AKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGE--NAEEKLRE 240
K V VGFG+S+RE VE L A+GVVVGSA++ ++ E + N EKL +
Sbjct: 195 VKTHASVPVVVGFGISRREQVEALAE-NADGVVVGSAIVKLVGELEDELNDPEKLED 250
>sp|P34816|TRPA_PSESY TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|476481|pir||JQ2127 tryptophan
synthase (EC 4.2.1.20) alpha chain - Pseudomonas
syringae >gi|151619|gb|AAA26013.1| (M95710) tryptophan
synthase alpha subunit [Pseudomonas syringae]
Length = 269
Score = 164 bits (410), Expect = 1e-39
Identities = 91/225 (40%), Positives = 134/225 (59%), Gaps = 3/225 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ ++TAGDP +L L + IELG+PF+DP+ADG IQ ++ RAL+
Sbjct: 18 ALVTFVTAGDPGYDTSLAILKGLPAAGADVIELGMPFTDPMADGPAIQLANIRALEAKQN 77
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +VREFRK ++D P+VLM Y+NP+ + G+ +FI AK++GVDG+++VD+P H
Sbjct: 78 LLKTLQMVREFRKGNNDTPLVLMGYFNPIHKYGVPKFIADAKEAGVDGLIVVDMPPEHNG 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
E + A+ GI + L P T D RL + S+GFVY +S+ G TGA E +
Sbjct: 138 ELCDPAQAAGIDFIRLTTPTTDDVRLPTVLNGSSGFVYYVSVAGVTGAGAATLEHVEEAV 197
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIA 228
R +R +++GFG+ E + A+GVVVGSALI+ IA
Sbjct: 198 ARLRRHTDLPISIGFGIRTPEQAAAIARL-ADGVVVGSALIDHIA 241
>sp|P22095|TRPA_VIBPA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|48491|emb|CAA35036.1| (X17149)
tryptophan synthase; alpha subunit [Vibrio
parahaemolyticus]
Length = 268
Score = 163 bits (408), Expect = 2e-39
Identities = 87/243 (35%), Positives = 142/243 (57%), Gaps = 3/243 (1%)
Query: 5 GSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGF 63
G+ +P++T DP+A+ + + + +E + A+ELGIPFSDP+ADG TIQ ++ RAL G
Sbjct: 17 GAFVPFVTVCDPNAEQSYKIMETLVESGADALELGIPFSDPLADGPTIQGANIRALDSGA 76
Query: 64 KLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHA 122
+ F + + R K+ D+PI L+ Y N V+ G+ F + +G+D +LI D+P +
Sbjct: 77 TPDICFEQIGKIRAKYPDLPIGLLMYANLVYSRGIESFYERCAKAGIDSVLIADVPTNES 136
Query: 123 SEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNL 182
+EF+ A + GI +F+A P DE LK++ + G+ YL+S G TGA K ++
Sbjct: 137 AEFVAAAEKFGIHPIFIAPPTASDETLKQVSELGGGYTYLLSRAGVTGAETKANMPVDHM 196
Query: 183 LKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKV 242
L++ + +GFG+S+ V+ + AGA G + GSA++ II H E + L +K+
Sbjct: 197 LEKLNQFDAPPALLGFGISEPAQVKQAIEAGAAGAISGSAVVKIIEAHVEQPQIML-DKL 255
Query: 243 REL 245
EL
Sbjct: 256 GEL 258
>pir||E75455 tryptophan synthase, alpha subunit - Deinococcus radiodurans
(strain R1) >gi|6458667|gb|AAF10519.1|AE001947_2
(AE001947) tryptophan synthase, alpha subunit
[Deinococcus radiodurans]
Length = 270
Score = 162 bits (406), Expect = 3e-39
Identities = 88/228 (38%), Positives = 134/228 (58%), Gaps = 10/228 (4%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
+ IP++T G P A + + +E+GIP+SDP+ DG TIQ++ +AL GG
Sbjct: 30 AFIPFMTGGYPDAARFGEVAGDLLARADIMEVGIPYSDPLGDGPTIQRASEQALAGGTST 89
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
+VRE R+ D PIV+MTY NP++ VG EF+ A+++GVDG+++ DLP E
Sbjct: 90 RRTLELVRELRQKDDTPIVIMTYINPIYAVGPAEFMRLAQEAGVDGLILPDLPPDQDLEI 149
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARD-----KIPETAF 180
++A + G+ FL AP + ER+K + +A TGF+Y +S+ G TGAR+ ++P
Sbjct: 150 ADLAAQHGLAVTFLIAPTSTPERVKLVAEACTGFLYAVSVTGVTGAREGAALGEVP---- 205
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIA 228
+L A++ + V VGFGV + + A+GVVVGSA IN +A
Sbjct: 206 RMLDLARQYAQRPVVVGFGVKDAATAQQVAQV-ADGVVVGSAFINAVA 252
>gb|AAB60041.1| (U23495) tryptophan synthase alpha subunit [Escherichia coli]
Length = 268
Score = 162 bits (405), Expect = 5e-39
Identities = 84/238 (35%), Positives = 134/238 (56%), Gaps = 2/238 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLT 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKL 238
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H E+ L
Sbjct: 195 HLVAKLKEYNAAPPLQGFGISSPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKML 252
>gb|AAB60056.1| (U23499) tryptophan synthase alpha subunit [Escherichia coli]
Length = 268
Score = 162 bits (405), Expect = 5e-39
Identities = 84/238 (35%), Positives = 134/238 (56%), Gaps = 2/238 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEVLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAECEKVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKL 238
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H E+ L
Sbjct: 195 HLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKML 252
>gb|AAB60052.1| (U23498) tryptophan synthase alpha subunit [Escherichia coli]
>gi|1185401|gb|AAA87794.1| (U25884) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|1185406|gb|AAA87798.1| (U25885) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|1185411|gb|AAA87802.1| (U25886) tryptophan synthase
alpha subunit [Escherichia coli]
Length = 268
Score = 161 bits (403), Expect = 8e-39
Identities = 84/238 (35%), Positives = 134/238 (56%), Gaps = 2/238 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAECEKVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKL 238
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H E+ L
Sbjct: 195 HLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKML 252
>sp|P00928|TRPA_ECOLI TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|68329|pir||TSECA tryptophan
synthase (EC 4.2.1.20) alpha chain [validated] -
Escherichia coli >gi|43184|emb|CAA23662.1| (V00364)
reading frame trpA [Escherichia coli]
>gi|43206|emb|CAA23675.1| (V00372) trpA [Escherichia
coli] >gi|147959|gb|AAA57301.1| (J01714) tryptophan
synthase alpha subunit [Escherichia coli]
>gi|775126|gb|AAB60035.1| (U23489) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|775133|gb|AAA65140.1| (U23490) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|775140|gb|AAA65146.1| (U23491) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|775147|gb|AAA65152.1| (U23492) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|775161|gb|AAA65164.1| (U23494) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924764|gb|AAA73791.1| (U25417) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924771|gb|AAA73797.1| (U25418) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924778|gb|AAA73803.1| (U25419) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924785|gb|AAA73809.1| (U25420) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924792|gb|AAA73815.1| (U25421) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924799|gb|AAA73821.1| (U25422) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|1742049|dbj|BAA14792.1| (D90763) Tryptophan synthase
a chain (EC 4.2.1.20). [Escherichia coli]
>gi|1787514|gb|AAC74342.1| (AE000224) tryptophan
synthase, alpha protein [Escherichia coli K12]
>gi|2055397|gb|AAB53207.1| (U90436) tryptophan synthase
alpha subunit [synthetic construct]
Length = 268
Score = 161 bits (403), Expect = 8e-39
Identities = 84/238 (35%), Positives = 134/238 (56%), Gaps = 2/238 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKL 238
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H E+ L
Sbjct: 195 HLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKML 252
>gb|AAA65158.1| (U23493) tryptophan synthase alpha subunit [Escherichia coli]
Length = 268
Score = 161 bits (403), Expect = 8e-39
Identities = 84/238 (35%), Positives = 134/238 (56%), Gaps = 2/238 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKL 238
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H E+ L
Sbjct: 195 HLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKML 252
>gb|AAB60046.1| (U23497) tryptophan synthase alpha subunit [Escherichia coli]
Length = 268
Score = 161 bits (403), Expect = 8e-39
Identities = 84/238 (35%), Positives = 134/238 (56%), Gaps = 2/238 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKL 238
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H E+ L
Sbjct: 195 HLVTKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKML 252
>gb|AAA65176.1| (U23500) tryptophan synthase alpha subunit [Escherichia coli]
>gi|924806|gb|AAA73827.1| (U25423) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924813|gb|AAA73833.1| (U25425) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924820|gb|AAA73839.1| (U25426) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924827|gb|AAA73845.1| (U25427) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924834|gb|AAA73851.1| (U25428) tryptophan synthase
alpha subunit [Escherichia coli]
>gi|924841|gb|AAA73857.1| (U25429) tryptophan synthase
alpha subunit [Escherichia coli]
Length = 268
Score = 161 bits (402), Expect = 1e-38
Identities = 83/238 (34%), Positives = 134/238 (55%), Gaps = 2/238 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+P+
Sbjct: 75 GVTPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPIE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKL 238
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H E+ L
Sbjct: 195 HLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQHINEPEKML 252
>sp|P56141|TRPA_HELPY TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436990|pir||E64679 tryptophan
synthase, alpha subunit - Helicobacter pylori (strain
26695) >gi|2314444|gb|AAD08322.1| (AE000632) tryptophan
synthase, alpha subunit (trpA) [Helicobacter pylori
26695]
Length = 262
Score = 161 bits (402), Expect = 1e-38
Identities = 87/245 (35%), Positives = 147/245 (59%), Gaps = 6/245 (2%)
Query: 6 SLIPYLTAGDPSAKATLRFL--LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGF 63
+ IP++T GDP+ + + + L I S A+ELG+ FSDP+ADG TIQ SH RALK
Sbjct: 17 AFIPFVTLGDPNYELSFEIIKTLIISGVS-ALELGLAFSDPVADGITIQASHLRALKHAS 75
Query: 64 KLEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHA 122
++ F ++++ R ++ ++PI L+ Y N +F G+ F +AK+ G+D +LI D+P++
Sbjct: 76 MAKN-FQLLKKIRDYNHNIPIGLLAYANLIFSYGVDGFYAQAKECGIDSVLIADMPLIEK 134
Query: 123 SEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNL 182
++ A++ IK +F+A+PN + L+++ S G++Y ++ G TGA + + +
Sbjct: 135 ELVIKSAQKHQIKQIFIASPNASSKDLEQVATHSQGYIYALARSGVTGASRILENDSSAI 194
Query: 183 LKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKV 242
+K K +GFG+SK+EH+ GA+GV+ GSAL+ II E+ N E + EK+
Sbjct: 195 IKTLKAFSPTPALLGFGISKKEHITNAKGMGADGVICGSALVKIIEENLNN-ENAMLEKI 253
Query: 243 RELAG 247
+ G
Sbjct: 254 KGFIG 258
>pir||B81934 probable tryptophan synthase alpha chain NMA0879 [imported] -
Neisseria meningitidis (group A strain Z2491)
>gi|7379594|emb|CAB84159.1| (AL162754) putative
tryptophan synthase alpha chain [Neisseria meningitidis
Z2491]
Length = 261
Score = 161 bits (402), Expect = 1e-38
Identities = 89/242 (36%), Positives = 134/242 (54%), Gaps = 3/242 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LIPY+T GDP + TL + + + +ELG+PFSDP+ADG IQ++ RAL G
Sbjct: 16 ALIPYITVGDPDIRTTLALMHGMVANGADILELGVPFSDPMADGPVIQRAAERALANGIS 75
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L ++VR+FR+ + P+VLM Y NPV ++G REF +A +GVDG+L VD PV
Sbjct: 76 LRDVLDVVRKFRETDTQTPVVLMGYLNPVHKMGYREFAQEAAKAGVDGVLTVDSPVETID 135
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
++ G+ +FL AP T ++R+K I + + GFVY +SL G TGA + +
Sbjct: 136 PLYRELKDNGVDCIFLIAPTTTEDRIKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKI 195
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKVR 243
+ + + VGFG+S E + A V+VGS ++ I + N + V+
Sbjct: 196 EYLHQYIDIPIGVGFGISNAESARK-IGRVAEAVIVGSRIVKEIENNAGNEAAVVGALVK 254
Query: 244 EL 245
EL
Sbjct: 255 EL 256
>pir||D82232 tryptophan synthase, alpha chain VC1169 [imported] - Vibrio
cholerae (group O1 strain N16961)
>gi|9655645|gb|AAF94328.1| (AE004197) tryptophan
synthase, alpha subunit [Vibrio cholerae]
Length = 268
Score = 160 bits (400), Expect = 2e-38
Identities = 83/240 (34%), Positives = 140/240 (57%), Gaps = 2/240 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
+ G+ +P++T GDP+ + +L + I+ + A+ELG+PFSDP+ADG TIQ ++ RAL
Sbjct: 15 QQGAFVPFVTIGDPNPEQSLAIMQTLIDAGADALELGMPFSDPLADGPTIQGANLRALAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
+ F ++ + R ++ + PI L+ Y N V+ G+ +F + + +GVD +LI D+P
Sbjct: 75 KTTPDICFELIAQIRARNPETPIGLLMYANLVYARGIDDFYQRCQKAGVDSVLIADVPTN 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
+ F+ A + GI+ +F+A P DE L+ + + G+ YL+S G TGA K
Sbjct: 135 ESQPFVAAAEKFGIQPIFIAPPTASDETLRAVAQLGKGYTYLLSRAGVTGAETKANMPVH 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
LL+R ++ +GFG+S+ V+ + AGA G + GSA++ II H +N ++L E
Sbjct: 195 ALLERLQQFDAPPALLGFGISEPAQVKQAIEAGAAGAISGSAVVKIIETHLDNPAKQLTE 254
>gb|AAA65170.1| (U23496) tryptophan synthase alpha subunit [Escherichia coli]
Length = 268
Score = 159 bits (399), Expect = 2e-38
Identities = 82/230 (35%), Positives = 131/230 (56%), Gaps = 2/230 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 KEGAFVPFVTLGDPGIEQSLKIIDTLIEAGADALELGIPFSDPLADGPTIQNATLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ EF + + GVD +L+ D+PV
Sbjct: 75 GVPPAQCFEMLALIRQKHPTIPIGLLMYANLVFNKGIDEFYAQCEKVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A + +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEH 230
+L+ + K GFG+S + V+ ++AGA G + GSA++ II +H
Sbjct: 195 HLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISGSAIVKIIEQH 244
>sp|O68429|TRPA_BUCDN TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|3170098|gb|AAC27736.1|
(AF038565) tryptophan synthase small subunit [Buchnera
aphidicola]
Length = 270
Score = 159 bits (399), Expect = 2e-38
Identities = 82/230 (35%), Positives = 138/230 (59%), Gaps = 2/230 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G +P++ GDPS +L+ + + I+ + A+ELGIPFSDP+ADGKTIQ+++ RAL
Sbjct: 15 KEGCFVPFVVLGDPSLDTSLKIINILIQNGADALELGIPFSDPLADGKTIQKANLRALSQ 74
Query: 62 GFKLEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
+ F ++ RK H+ +PI L+ Y N V+ GL F K + SGVD +LI D+P+
Sbjct: 75 KNNIFQYFKEIKNLRKKHTQIPIGLLIYANLVYNQGLDNFYLKCRKSGVDSVLIADVPIE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
+ F A + I ++F+ PN D+ L I + G++Y++S G TG ++ +
Sbjct: 135 ESEIFYTTANKYKISSIFICPPNADDDLLYRISLYAKGYIYVLSRPGVTGIENQNFFVSG 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEH 230
+ +K+ K+ + GFG+S V+ +++G +GV+ GSA+INII ++
Sbjct: 195 DFIKKIKKYNSVPLLQGFGISNSIQVKQAISSGLSGVICGSAIINIIEKY 244
>sp|P00929|TRPA_SALTY TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|68330|pir||TSEBAT tryptophan
synthase (EC 4.2.1.20) alpha chain - Salmonella
typhimurium >gi|11513799|pdb|1QOQ|A Chain A, Crystal
Structure Of Wild-Type Tryptophan Synthase Complexed
With Indole Glycerol Phosphate >gi|6730203|pdb|1CW2|A
Chain A, Crystal Structure Of The Complex Of Bacterial
Tryptophan Synthase With The Transition State Analogue
Inhibitor 4-(2- Hydroxyphenylsulfinyl)-Butylphosphonic
Acid >gi|6980600|pdb|1C8V|A Chain A, Crystal Structure
Of The Complex Of Bacterial Tryptophan Synthase With The
Transition State Analogue Inhibitor 4-(2-
Hydroxyphenylthio)-Butylphosphonic Acid
>gi|6980564|pdb|1C29|A Chain A, Crystal Structure Of The
Complex Of Bacterial Tryptophan Synthase With The
Transition State Analogue Inhibitor 4-(2-
Hydroxyphenylthio)-1-Butenylphosphonic Acid
>gi|6730159|pdb|1C9D|A Chain A, Crystal Structure Of The
Complex Of Bacterial Tryptophan Synthase With The
Transition State Analogue Inhibitor 4-(2-
Hydroxy-4-Fluorophenylthio)-Butylphosphonic Acid
>gi|6730206|pdb|1CX9|A Chain A, Crystal Structure Of The
Complex Of Bacterial Tryptophan Synthase With The
Transition State Analogue Inhibitor 4-(2-
Aminophenylthio)-Butylphosphonic Acid
>gi|2098383|pdb|2TRS|A Chain A, Crystal Structures Of
Mutant (Betak87t) Tryptophan Synthase Alpha2 Beta2
Complex With Ligands Bound To The Active Sites Of The
Alpha And Beta Subunits Reveal Ligand-Induced
Conformational Changes >gi|4699587|pdb|1A50|A Chain A,
Crystal Structure Of Wild-Type Tryptophan Synthase
Complexed With 5-Fluoroindole Propanol Phosphate
>gi|4699590|pdb|1A5S|A Chain A, Crystal Structure Of
Wild-Type Tryptophan Synthase Complexed With
5-Fluoroindole Propanol Phosphate And L-Ser Bound As
Amino Acrylate To The Beta Site >gi|1431671|pdb|1UBS|A
Chain A, Tryptophan Synthase (E.C.4.2.1.20) With A
Mutation Of Lys 87 ->thr In The B Subunit And In The
Presence Of Ligand L-Serine >gi|1421254|pdb|1TTQ|A Chain
A, Tryptophan Synthase (E.C.4.2.1.20) In The Presence Of
Potassium At Room Temperature >gi|1421252|pdb|1TTP|A
Chain A, Tryptophan Synthase (E.C.4.2.1.20) In The
Presence Of Cesium, Room Temperature
>gi|2098381|pdb|2TYS|A Chain A, Crystal Structures Of
Mutant (Betak87t) Tryptophan Synthase Alpha2 Beta2
Complex With Ligands Bound To The Active Sites Of The
Alpha And Beta Subunits Reveal Ligand-Induced
Conformational Changes >gi|4699723|pdb|1BKS|A Chain A,
Tryptophan Synthase (E.C.4.2.1.20) From Salmonella
Typhimurium >gi|4699619|pdb|2WSY|A Chain A, Crystal
Structure Of Wild-Type Tryptophan Synthase
>gi|47940|emb|CAA24666.1| (V01376) reading frame (trpA)
[Salmonella typhimurium] >gi|154386|gb|AAA27235.1|
(J01810) trpa [Salmonella typhimurium]
Length = 268
Score = 159 bits (398), Expect = 3e-38
Identities = 82/240 (34%), Positives = 135/240 (56%), Gaps = 2/240 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
R+G+ +P++T GDP + +L+ + I+ + A+ELG+PFSDP+ADG TIQ ++ RA
Sbjct: 15 REGAFVPFVTLGDPGIEQSLKIIDTLIDAGADALELGVPFSDPLADGPTIQNANLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ F + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIREKHPTIPIGLLMYANLVFNNGIDAFYARCEQVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A I +F+ PN D+ L+++ G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNIAPIFICPPNADDDLLRQVASYGRGYTYLLSRSGVTGAENRGALPLH 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+L+++ K GFG+S E V + AGA G + GSA++ II ++ + ++ L E
Sbjct: 195 HLIEKLKEYHAAPALQGFGISSPEQVSAAVRAGAAGAISGSAIVKIIEKNLASPKQMLAE 254
>pdb|1QOP|A Chain A, Crystal Structure Of Wild-Type Tryptophan Synthase
Complexed With Indole Propanol Phosphate
Length = 268
Score = 159 bits (398), Expect = 3e-38
Identities = 82/240 (34%), Positives = 135/240 (56%), Gaps = 2/240 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
R+G+ +P++T GDP + +L+ + I+ + A+ELG+PFSDP+ADG TIQ ++ RA
Sbjct: 15 REGAFVPFVTLGDPGIEQSLKIIDTLIDAGADALELGVPFSDPLADGPTIQNANLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ F + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLAIIREKHPTIPIGLLMYANLVFNNGIDAFYARCEQVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A I +F+ PN D+ L+++ G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNIAPIFICPPNADDDLLRQVASYGRGYTYLLSRSGVTGAENRGALPLH 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+L+++ K GFG+S E V + AGA G + GSA++ II ++ + ++ L E
Sbjct: 195 HLIEKLKEYHAAPALQGFGISSPEQVSAAVRAGAAGAISGSAIVKIIEKNLASPKQMLAE 254
>gb|AAF14255.1|AF132318_5 (AF132318) alpha subunit of tryptophan synthetase [Buchnera
aphidicola]
Length = 270
Score = 159 bits (397), Expect = 4e-38
Identities = 84/241 (34%), Positives = 138/241 (56%), Gaps = 2/241 (0%)
Query: 2 FRDGSLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALK 60
F+ G IP++ GDPS +L+ + A IE + +ELGIPFSDPIADG+ IQ+++ RA
Sbjct: 14 FKKGCFIPFVVLGDPSIDMSLKIINALIENGADGLELGIPFSDPIADGEIIQKANLRAFN 73
Query: 61 GGFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
L F+I+ E R KH +PI L+ Y N +F+ G+ +F K G+D +LI DLP+
Sbjct: 74 SKINLHKCFDILSEIREKHQTIPIGLLLYSNLIFKFGINKFYLKCYKIGIDSILIADLPI 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
+ F + A I VF+ P+ +K I S G++YL+S G TG +I
Sbjct: 134 EESYVFRKYAVINNILPVFICPPDAKKNVIKNIAIHSQGYIYLLSRSGVTGINQEIMIPP 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLR 239
+L+ + K++ K + GFGVS ++ ++ +G +GV+ GS + +I + ++ ++ ++
Sbjct: 194 LSLINKLKKLTKTPLIQGFGVSYPYQIQKIILSGISGVICGSIIAKLIENYFQDDDKLIK 253
Query: 240 E 240
E
Sbjct: 254 E 254
>gi|6321412 tryptophan synthetase; Trp5p [Saccharomyces cerevisiae]
>gi|136373|sp|P00931|TRP_YEAST TRYPTOPHAN SYNTHASE
>gi|68333|pir||TSBYAB tryptophan synthase (EC 4.2.1.20)
- yeast (Saccharomyces cerevisiae)
>gi|4674|emb|CAA24635.1| (V01342) trp5 [Saccharomyces
cerevisiae] >gi|1322496|emb|CAA96727.1| (Z72548) ORF
YGL026c [Saccharomyces cerevisiae]
Length = 707
Score = 158 bits (395), Expect = 7e-38
Identities = 89/230 (38%), Positives = 133/230 (57%), Gaps = 4/230 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYS-GAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ ++TAG P+ K T+ L ++ IELG+PFSDPIADG TIQ S+ AL+ G
Sbjct: 19 ALVTFMTAGYPTVKDTVPILKGFQDGGVDIIELGMPFSDPIADGPTIQLSNTVALQNGVT 78
Query: 65 LEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +V + R VPI+LM YYNP+ G FI A +G +G +IVDLP A
Sbjct: 79 LPQTLEMVSQARNEGVTVPIILMGYYNPILNYGEERFIQDAAKAGANGFIIVDLPPEEAL 138
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
+ + G+ + L AP+T DERL+ + + FVY++S GTTG + + L+
Sbjct: 139 KVRNYINDNGLSLIPLVAPSTTDERLELLSHIADSFVYVVSRMGTTGVQSSVASDLDELI 198
Query: 184 KRAKRICKN-KVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGE 232
R ++ K+ +AVGFGVS REH + + + A+GVV+GS ++ + + E
Sbjct: 199 SRVRKYTKDTPLAVGFGVSTREHFQS-VGSVADGVVIGSKIVTLCGDAPE 247
>pir||D81171 tryptophan synthase, alpha chain NMB0678 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7225905|gb|AAF41096.1| (AE002422) tryptophan
synthase, alpha subunit [Neisseria meningitidis MC58]
Length = 261
Score = 157 bits (394), Expect = 9e-38
Identities = 88/242 (36%), Positives = 134/242 (55%), Gaps = 3/242 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LI Y+T GDP + TL + + + +ELG+PFSDP+ADG IQ++ RAL G
Sbjct: 16 ALIAYITVGDPDIRTTLALMHGMVANGADILELGVPFSDPMADGPVIQRAAERALANGIS 75
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L ++VR+FR+ + P+VLM Y NPV ++G REF +A +GVDG+L VD PV
Sbjct: 76 LRDVLDVVRKFRETDTQTPVVLMGYLNPVHKMGYREFAQEAAKAGVDGVLTVDSPVETID 135
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
++ G+ +FL AP T ++R+K I + + GFVY +SL G TGA + +
Sbjct: 136 PLYRELKDNGVDCIFLIAPTTTEDRIKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKI 195
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKVR 243
+ + + VGFG+S E + A+ V+VGS ++ I + N + V+
Sbjct: 196 EYLHQYIDIPIGVGFGISNAESARK-IGRVADAVIVGSRIVKEIENNTGNEAAAVGALVK 254
Query: 244 EL 245
EL
Sbjct: 255 EL 256
>sp|P00930|TRPA_KLEAE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|43784|emb|CAA23902.1| (V00630)
reading frame trpA [Klebsiella aerogenes]
>gi|149328|gb|AAA25145.1| (J01738) trpa [Klebsiella
aerogenes]
Length = 269
Score = 157 bits (393), Expect = 1e-37
Identities = 85/240 (35%), Positives = 132/240 (54%), Gaps = 2/240 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G+ +P++T GDP + +L+ + A IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 QEGAFVPFVTLGDPGPEQSLKIIDALIEGGADALELGIPFSDPLADGPTIQGAALRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ F + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLAAIRQKHPTIPIGLLMYANLVFSPGIDAFYAQCARVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A I +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAAMRHNIAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLH 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+L+++ GFG+S E V ++AGA G + GSA++ II H + + L E
Sbjct: 195 HLVEKLAEYHAAPPLQGFGISAPEQVSAAIDAGAAGAISGSAIVKIIERHLDEPQTMLDE 254
>sp|P12291|TRPA_CAUCR TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|279953|pir||D43664 tryptophan
synthase (EC 4.2.1.20) alpha chain - Caulobacter
crescentus >gi|144288|gb|AAA23058.1| (M19129) tryptophan
synthase A protein (trpA; gtg start codon; EC 4.2.1.20)
[Caulobacter crescentus]
Length = 275
Score = 157 bits (393), Expect = 1e-37
Identities = 97/245 (39%), Positives = 134/245 (54%), Gaps = 8/245 (3%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
+ Y+ AGDP A TL L + +GA IELG PFSDP+A+G TIQ++ R LK G L
Sbjct: 22 VTYVMAGDPDAATTLSVLKGLPA-AGADLIELGFPFSDPMAEGPTIQRAAQRGLKSGMTL 80
Query: 66 EHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
+ ++V FR+ D PI+LM Y NPV G F A +GVDG++IVD P A
Sbjct: 81 QGTLDLVAAFREGDQDTPIILMGYLNPVLNKGFETFAALAAKAGVDGLIIVDCPPEEADP 140
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
+ EGI + LAAP T D RL + + ++GFVY +S+ G TG + ++
Sbjct: 141 LSDALEAEGIALIRLAAPTTDDARLPMVVRRTSGFVYYVSVAGVTGVKSADAADVAPAVE 200
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINII---AEHGENAEEKLREK 241
R ++ VAVGFG+ + + A+ VVGSAL++ I A+ EN EK+ K
Sbjct: 201 RLRKASGLPVAVGFGIRTPDQAAAVAKV-ADAAVVGSALVDEIESAAQLNENVTEKVLLK 259
Query: 242 VRELA 246
ELA
Sbjct: 260 ASELA 264
>gi|11465848 tryptophan synthase alpha subunit [Porphyra purpurea]
>gi|1717756|sp|P51382|TRPA_PORPU TRYPTOPHAN SYNTHASE
ALPHA CHAIN >gi|2148111|pir||S73303 tryptophan synthase
(EC 4.2.1.20) alpha chain - red alga (Porphyra
purpurea) chloroplast >gi|1276848|gb|AAC08268.1|
(U38804) tryptophan synthase alpha subunit [Porphyra
purpurea]
Length = 273
Score = 157 bits (393), Expect = 1e-37
Identities = 81/236 (34%), Positives = 137/236 (57%), Gaps = 3/236 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LIP++TAGDP +T + L ++++ IELG+P+SDP+ADG IQ + RALK
Sbjct: 26 ALIPFITAGDPDLVSTSKALKILDQHGADIIELGLPYSDPLADGPIIQAASSRALKQSIN 85
Query: 65 LEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
L + ++V + PIVL TYYNPV +G+ FI +G+ G+LI DLP+ +
Sbjct: 86 LNNILDMVNITSSNIVAPIVLFTYYNPVLNLGINNFISAISRAGIKGLLIPDLPIEESDY 145
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
+ V + I+ +FL +P + ER+ +I + + G +YL+S G TG + ++ L +
Sbjct: 146 IISVCKLFNIELIFLLSPTSSIERINKIVEQAPGCIYLVSTTGVTGQKPELTGKLKRLTE 205
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
K++ + + +GFG+S E ++ + NG+V+GSA + ++ + EE LR+
Sbjct: 206 TIKKMTQKPIILGFGISTAEQIKEIKGWNINGIVIGSAFVKRLS--SDFPEEGLRK 259
>pdb|1A5A|A Chain A, Cryo-Crystallography Of A True Substrate,
Indole-3-Glycerol Phosphate, Bound To A Mutant
(Alphad60n) Tryptophan Synthase Alpha2beta2 Complex
Reveals The Correct Orientation Of Active Site Alpha Glu
49 >gi|3659998|pdb|1BEU|A Chain A, Trp Synthase
(D60n-Ipp-Ser) With K+ >gi|3212367|pdb|1A5B|A Chain A,
Cryo-Crystallography Of A True Substrate,
Indole-3-Glycerol Phosphate, Bound To A Mutant (Alpha
D60n) Tryptophan Synthase Alpha2beta2 Complex Reveals
The Correct Orientation Of Active Site Alpha Glu 49
Length = 268
Score = 157 bits (393), Expect = 1e-37
Identities = 81/240 (33%), Positives = 135/240 (55%), Gaps = 2/240 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
R+G+ +P++T GDP + +L+ + I+ + A+ELG+PFSDP+A+G TIQ ++ RA
Sbjct: 15 REGAFVPFVTLGDPGIEQSLKIIDTLIDAGADALELGVPFSDPLANGPTIQNANLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ F + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIREKHPTIPIGLLMYANLVFNNGIDAFYARCEQVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A I +F+ PN D+ L+++ G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAALRHNIAPIFICPPNADDDLLRQVASYGRGYTYLLSRSGVTGAENRGALPLH 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+L+++ K GFG+S E V + AGA G + GSA++ II ++ + ++ L E
Sbjct: 195 HLIEKLKEYHAAPALQGFGISSPEQVSAAVRAGAAGAISGSAIVKIIEKNLASPKQMLAE 254
>emb|CAA79517.1| (Z19090) tryptophan synthase; alpha subunit [Vibrio metschnikovii]
Length = 268
Score = 157 bits (393), Expect = 1e-37
Identities = 86/237 (36%), Positives = 133/237 (55%), Gaps = 2/237 (0%)
Query: 5 GSLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGF 63
G+ +P++T GDP+ + +LR + +E + A+ELGIPFSDP+ADG TIQ + AL G
Sbjct: 17 GAFVPFVTIGDPNPQQSLRIMQTLVEAGADALELGIPFSDPLADGPTIQGA-ISALDSGT 75
Query: 64 KLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
K F + + D+PI L+ Y N V+ G+ F + + +GVD +LI D+P ++
Sbjct: 76 KPIRCFEADQAPIRAQDIPIYLLMYANLVYARGIDNFYQRCQQAGVDSVLIADVPTNESA 135
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
EF A++ I +F+A P DE L+ + + +G+ YL+S G TGA K LL
Sbjct: 136 EFGPAAKKYAIHPIFIAPPTASDETLQSVAELGSGYTYLLSRSGVTGAETKANMPVHALL 195
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+R + +GFG+S+ E V+ + +GA G + GSA++ II H E L E
Sbjct: 196 ERLNQFSAPPRRLGFGISEPEQVKQAIESGAAGAISGSAVVKIIEHHLAKPEAMLAE 252
>sp|P13228|TRP_NEUCR TRYPTOPHAN SYNTHASE >gi|101877|pir||A32959 tryptophan synthase (EC
4.2.1.20) - Neurospora crassa >gi|168916|gb|AAA33616.1|
(J04594) tryptophan synthetase [Neurospora crassa]
Length = 708
Score = 157 bits (392), Expect = 2e-37
Identities = 93/235 (39%), Positives = 135/235 (56%), Gaps = 4/235 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ Y+TAG P + T LLA+E+ IELG+PF+DPIADG TIQ ++ AL+ G
Sbjct: 18 ALVTYVTAGFPHPEQTPDILLAMEKGGADVIELGVPFTDPIADGPTIQTANTIALQHGVT 77
Query: 65 LEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L+ +VR+ R+ P++LM YYNP+ G + K++GV+G +IVDLP A
Sbjct: 78 LQSTLQMVRDARQRGLKAPVMLMGYYNPLLSYGEERLLNDCKEAGVNGFIIVDLPPEEAV 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
F ++ G+ V L AP T D R++ + + + F+Y++S G TGA + LL
Sbjct: 138 SFRQLCTRGGLSYVPLIAPATSDARMRVLCQLADSFIYVVSRQGVTGASGTLNANLPELL 197
Query: 184 KRAKRICKNK-VAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEK 237
R K+ NK AVGFGVS +H + A A+GVVVGS +I I + + E K
Sbjct: 198 ARVKKYSGNKPAAVGFGVSTHDHFTQ-VGAIADGVVVGSMIITTIQKAAKGEEVK 251
>pdb|2TSY|A Chain A, Crystal Structures Of Mutant (Betak87t) Tryptophan
Synthase Alpha2 Beta2 Complex With Ligands Bound To The
Active Sites Of The Alpha And Beta Subunits Reveal
Ligand-Induced Conformational Changes
Length = 262
Score = 155 bits (389), Expect = 3e-37
Identities = 82/240 (34%), Positives = 134/240 (55%), Gaps = 8/240 (3%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
R+G+ +P++T GDP + +L+ + I+ + A+ELG+PFSDP+ADG TIQ ++ RA
Sbjct: 15 REGAFVPFVTLGDPGIEQSLKIIDTLIDAGADALELGVPFSDPLADGPTIQNANLRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ F + + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLALIREKHPTIPIGLLMYANLVFNNGIDAFYARCEQVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A I +F+ PN D+ L+++ G+ YL+S G TGA +
Sbjct: 135 ESAPFRQAALRHNIAPIFICPPNADDDLLRQVASYGRGYTYLLSRSGVTGAEN------H 188
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+L+++ K GFG+S E V + AGA G + GSA++ II ++ + ++ L E
Sbjct: 189 HLIEKLKEYHAAPALQGFGISSPEQVSAAVRAGAAGAISGSAIVKIIEKNLASPKQMLAE 248
>sp|Q9X4E8|TRPA_RHOSH TRYPTOPHAN SYNTHASE ALPHA CHAIN
>gi|4761131|gb|AAD29264.1|AF107094_1 (AF107094)
tryptophan synthase alpha chain [Rhodobacter
sphaeroides]
Length = 263
Score = 155 bits (389), Expect = 3e-37
Identities = 90/237 (37%), Positives = 138/237 (57%), Gaps = 13/237 (5%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYS-GAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+ + Y+ AGDP + +L + + E IELG+PF+DP+ADG TIQ + RAL+GG
Sbjct: 18 AFVAYIMAGDPDLETSLAVMRGLPEAGVDIIELGMPFTDPMADGPTIQTAGQRALEGGQT 77
Query: 65 LEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +VR FR ++++ PIV+M YYNP++ G+ F+ +A ++G+DG+++VDLP +
Sbjct: 78 LTRTLEMVRAFRAENAETPIVMMGYYNPIYARGVETFLAEATEAGIDGLIVVDLPPEEDA 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTG-----ARDKIPET 178
E A+ G+ + LA P T RL ++ + ++GFVY +S+ G TG A D PE
Sbjct: 138 ELCLPAQAAGLNFIRLATPTTDSRRLPKVLQNTSGFVYYVSITGITGAAAAQAADVAPEV 197
Query: 179 AFNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAE 235
A R K V VGFG++ E + L A+G VVGSA++ ++ E AE
Sbjct: 198 A-----RIKAATDLPVIVGFGITTPEAAQDLAGI-ADGCVVGSAIVKLVGEGRPVAE 248
>sp|P31204|TRPA_ANTSP TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|421736|pir||S31843 tryptophan
synthase (EC 4.2.1.20) alpha chain - red alga
(Antithamnion sp.) chloroplast >gi|11331|emb|CAA79757.1|
(Z21642) tryptophane synthase [Antithamnion sp.]
Length = 263
Score = 155 bits (388), Expect = 4e-37
Identities = 76/220 (34%), Positives = 123/220 (55%), Gaps = 1/220 (0%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LIP++TAG P T++ L ++ IELGIP+SD +ADG IQ S AL+GG
Sbjct: 16 ALIPFITAGYPDINTTIQALYELDSQGADIIELGIPYSDALADGSVIQHSSLIALQGGTY 75
Query: 65 LEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
++ +I+ + PI+++ YYNP+ + G+ +FI + G G+++ DLP+ E
Sbjct: 76 IDQVLHILEVVSTKLNTPIIILPYYNPILKRGIEKFIKQISLMGAKGLIVPDLPLEETDE 135
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
+ + + I+ V AP + +R+ I K S G +YL+S G TG RD I L
Sbjct: 136 LIVICNDNQIELVLFVAPTSSMKRINSISKKSPGCIYLVSSTGVTGVRDDIDIKVMELSN 195
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALI 224
K++ + +GFG+S EH++ ++ +GVVVGSA +
Sbjct: 196 YIKKVSNKFIMLGFGISTPEHIKKIMKWNIDGVVVGSAFV 235
>pir||TSAEAA tryptophan synthase (EC 4.2.1.20) alpha chain - Klebsiella
pneumoniae
Length = 269
Score = 155 bits (387), Expect = 6e-37
Identities = 84/240 (35%), Positives = 131/240 (54%), Gaps = 2/240 (0%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++ + +P++T GDP + +L+ + A IE + A+ELGIPFSDP+ADG TIQ + RA
Sbjct: 15 QEAAFVPFVTLGDPGPEQSLKIIDALIEGGADALELGIPFSDPLADGPTIQGAALRAFAA 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G F ++ R KH +PI L+ Y N VF G+ F + GVD +L+ D+PV
Sbjct: 75 GVTPAQCFEMLAAIRQKHPTIPIGLLMYANLVFSPGIDAFYAQCARVGVDSVLVADVPVE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
++ F + A I +F+ PN D+ L++I G+ YL+S G TGA ++
Sbjct: 135 ESAPFRQAAMRHNIAPIFICPPNADDDLLRQIASYGRGYTYLLSRAGVTGAENRAALPLH 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+L+++ GFG+S E V ++AGA G + GSA++ II H + + L E
Sbjct: 195 HLVEKLAEYHAAPPLQGFGISAPEQVSAAIDAGAAGAISGSAIVKIIERHLDEPQTMLDE 254
>sp|P07601|TRPA_BACSU TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80434|pir||F22794 tryptophan
synthase (EC 4.2.1.20) alpha chain trpA - Bacillus
subtilis >gi|143773|gb|AAA22870.1| (K01391) TrpA protein
[Bacillus subtilis] >gi|143813|gb|AAA20866.1| (M80245)
TrpA [Bacillus subtilis] >gi|2634681|emb|CAB14179.1|
(Z99115) tryptophan synthase (alpha subunit) [Bacillus
subtilis] >gi|224466|prf||1106178B protein trpA
[Bacillus subtilis]
Length = 267
Score = 154 bits (386), Expect = 8e-37
Identities = 82/224 (36%), Positives = 135/224 (59%), Gaps = 4/224 (1%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
IP++TAGDP + ++ ++++ A+ELG+ +SDP+ADG IQ++ RAL G +
Sbjct: 14 IPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQRASKRALDQGMNIV 73
Query: 67 HAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
A + E +K+ ++PI+L TYYNPV ++ F +++ +DG+L+ DLP+ ++
Sbjct: 74 KAIELGGEMKKNGVNIPIILFTYYNPVLQLNKEYFFALLRENHIDGLLVPDLPLEESNSL 133
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKR 185
E + + + L AP T + RLK I + + GFVY +S G TG R++ + + ++
Sbjct: 134 QEECKSHEVTYISLVAP-TSESRLKTIIEQAEGFVYCVSSLGVTGVRNEFNSSVYPFIRT 192
Query: 186 AKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE 229
K + VAVGFG+S RE V + +N ++GVVVGSAL+ I E
Sbjct: 193 VKNLSTVPVAVGFGISNREQV-IKMNEISDGVVVGSALVRKIEE 235
>sp|O22018|TRPA_CYAME TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|2541878|dbj|BAA22814.1|
(D63675) tryptophan synthase alpha subunit
[Cyanidioschyzon merolae]
Length = 243
Score = 154 bits (385), Expect = 1e-36
Identities = 75/226 (33%), Positives = 137/226 (60%), Gaps = 2/226 (0%)
Query: 7 LIPYLTAGDPSAKATLRFLLAI-EEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
LI YLTAG P T ++ + ++ + IE+G+P+SDP+ADG +Q++ +AL GF L
Sbjct: 2 LIAYLTAGAPDINTTKEAVMKLAKKGADVIEIGVPYSDPLADGAILQKASKQALMNGFHL 61
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
+H +N++ E + +VP+V++ YYN ++ G+ +++ K V G+++ DLP +
Sbjct: 62 DHLWNLLSEVNE-IEVPLVILAYYNQIWHYGVEKWVKKLVAHYVKGLIVPDLPYEESKTL 120
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKR 185
++ G+ ++L +P T R +E+ +A ++Y+IS G TG + + L+
Sbjct: 121 RQICDRYGLNIIWLISPTTNKTRAQELARACKDWIYVISRTGVTGLETEFDKQIPKLIGE 180
Query: 186 AKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHG 231
K++ K +A+GFG+SK E V+++ + GA+GV++GSA + I+ E G
Sbjct: 181 LKKVTKAPIALGFGISKSEQVKLVKSWGADGVIIGSACMQILLEKG 226
>gb|AAF61458.1|AF139661_3 (AF139661) tryptophan synthase alpha subunit [Azospirillum
brasilense]
Length = 284
Score = 153 bits (383), Expect = 2e-36
Identities = 98/252 (38%), Positives = 141/252 (55%), Gaps = 14/252 (5%)
Query: 7 LIPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGFK 64
L+ ++TAGDP + T R +L +GA IELG+PFSDP+ADG IQ + RAL G
Sbjct: 29 LVTFITAGDPDLE-TCRAVLHGLPAAGADLIELGLPFSDPMADGPAIQAASLRALHAGTT 87
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
++VR FR+ +D P++LM YYNP+ G+ F+ A ++GVDG+++VDLP
Sbjct: 88 ARKTLDLVRGFRETDADTPVILMGYYNPIHAYGVDRFLADAIEAGVDGLIVVDLPPEEDE 147
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
E A + G+ + LA P T D+RL + + ++GFVY +S+ G TGA +
Sbjct: 148 ELCIPALKAGVNFIRLATPTTDDKRLPAVLQNTSGFVYYVSIAGITGAASADNAAVGAAV 207
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREK-- 241
+R KR VAVGFG+ E + A+ VVGSA++ +A G +A+ K R
Sbjct: 208 ERLKRHTDLPVAVGFGIKTPEQAAEVARV-ADAAVVGSAIVTRLA-GGLDADGKARPGLA 265
Query: 242 ------VRELAG 247
VRELAG
Sbjct: 266 EDVLGFVRELAG 277
>gb|AAD51339.1|AF173835_3 (AF173835) tryptophan synthetase alpha subunit [Zymomonas mobilis
subsp. pomaceae]
Length = 274
Score = 152 bits (381), Expect = 3e-36
Identities = 91/233 (39%), Positives = 131/233 (56%), Gaps = 3/233 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYS-GAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LI +LTAGDPS +AT L ++ E IELG+PFSDP+ADG IQ+++ RAL
Sbjct: 18 ALIAFLTAGDPSIEATPALLDSLVENGVDIIELGMPFSDPMADGPAIQKANLRALAAKTG 77
Query: 65 LEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
F I EFRK H + P++LM Y NP+ G F AKD+GVDG++ VD+P +
Sbjct: 78 YNDIFRIATEFRKRHPETPLILMGYANPMTFKGSDSFAASAKDAGVDGIICVDIPPEEDA 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
RE+G+ + LA P + + RLK I ++GF+Y +S+ G TG + + L
Sbjct: 138 TLGVAVREKGMDMIRLATPTSDEARLKIILDGASGFLYYVSVAGVTGLQQATEASIDAAL 197
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEE 236
R K + VAVGFG+ E + A+ VVVGSA ++ I + N ++
Sbjct: 198 DRIKSVTDLPVAVGFGIRSPEQAAAIAKK-ADAVVVGSAFVSCIEKAVTNRQD 249
>sp|P06562|TRPA_BRELA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80601|pir||G24723 tryptophan
synthase (EC 4.2.1.20) alpha chain - Corynebacterium
glutamicum >gi|39598|emb|CAA28628.1| (X04960) trpA (AA
1-281) [Corynebacterium glutamicum]
Length = 281
Score = 152 bits (379), Expect = 5e-36
Identities = 85/248 (34%), Positives = 139/248 (55%), Gaps = 7/248 (2%)
Query: 4 DGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGG 62
+G+ +P++ DPS + + + AIE + A+ELG+PFSDP+ADG T+ +SH RAL GG
Sbjct: 17 EGAFVPFIMLSDPSPEEAFQIISTAIERGADALELGVPFSDPVADGPTVAESHLRALDGG 76
Query: 63 FKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMH 121
++ A ++ R + +VPI ++ Y N F GL F + ++G D +L+ D+PV
Sbjct: 77 ATVDSALEQIKRVRAAYPEVPIGMLIYGNVPFTRGLDRFYQEFAEAGADSILLPDVPVRE 136
Query: 122 ASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGA-RDKIPETAF 180
+ F A GI +++A N ++ L+ + AS G++Y IS G TG R+ +
Sbjct: 137 GAPFSAAA---GIDPIYIAPANASEKTLEGVSAASKGYIYAISRDGVTGTERESSTDGLS 193
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
++ K+ + +GFG+S +HV + AGA+G + GSA+ IIA H E E
Sbjct: 194 AVVDNIKKFDGAPILLGFGISSPQHVADAIAAGASGAITGSAITKIIASHCE-GEHPNPS 252
Query: 241 KVRELAGL 248
+R++ GL
Sbjct: 253 TIRDMDGL 260
>sp|P17166|TRPA_LACCA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|80565|pir||JS0344 tryptophan
synthase (EC 4.2.1.20) alpha chain - Lactobacillus casei
>gi|216760|dbj|BAA00387.1| (D00496) trpA protein
[Lactobacillus casei]
Length = 266
Score = 151 bits (378), Expect = 7e-36
Identities = 85/241 (35%), Positives = 140/241 (57%), Gaps = 8/241 (3%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
IP++ A DP + T++ ++A+ + GA +ELGIPFSDP+ADG IQ + RA +
Sbjct: 17 IPFIVADDPDFETTVKNVVALAK-GGADIVELGIPFSDPVADGPVIQAADLRAFAANVRT 75
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
+ F+IV RK + VPIV +TY N VF+ G F+ + D V G++I DLP E
Sbjct: 76 KTVFDIVEAARKETAVPIVFLTYLNIVFKYGYDAFLKRCADLNVAGLVIPDLPYESRDEI 135
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKR 185
+ +A + GI + L P T R+++I K+++GF+Y++S G TG RD+ L+
Sbjct: 136 VPIAEKYGIDIIPLITP-TSGHRIEKIAKSASGFIYVVSSMGITGERDEFFAGLKALVAE 194
Query: 186 AKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAE---EKLREKV 242
K+ A+GFG+ E + + A+GV++GSA+++++A+ + A EK +++
Sbjct: 195 IKQYTNVPTAIGFGIHTPEQAQTMAGI-ADGVIIGSAIVDLVAKEKQQAPAAIEKFTKQI 253
Query: 243 R 243
R
Sbjct: 254 R 254
>sp|P57365|TRPA_BUCAI TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|10038952|dbj|BAB12987.1|
(AP001118) tryptophan synthase alpha chain [Buchnera sp.
APS]
Length = 269
Score = 149 bits (372), Expect = 3e-35
Identities = 77/248 (31%), Positives = 147/248 (59%), Gaps = 3/248 (1%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G IP++ GDPS + +++ + I+ + A+E+GIPFSDP+ADG T+Q+S+ RAL
Sbjct: 15 KEGCFIPFVVLGDPSLETSIKIIETLIKSGADALEIGIPFSDPLADGPTVQKSNLRALSK 74
Query: 62 GFKLEHAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
F I+++ RK + +PI ++ Y N V+ G+ F + +SG+D +LI D+P+
Sbjct: 75 NNTFFEYFKILKQLRKKNKKLPIGILIYANLVYNQGIDNFYFQCFNSGLDSVLIADVPIE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
+ F +A + I +F+ P+ + L +I + GF+Y++S G TG ++
Sbjct: 135 ESKIFYNIANKYKINPIFICPPDADADFLYKISLYAQGFIYVLSRPGVTGIKNNTIALPR 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+ + + K+ + GFG+S + ++ +++G +GV+ GSA+INII ++ N E+K+ +
Sbjct: 195 DFINKIKKYNSVPLLQGFGISNPKQIKEAISSGLSGVICGSAIINIIEKY-LNQEKKMIK 253
Query: 241 KVRELAGL 248
++++ L
Sbjct: 254 EIKKFTHL 261
>gb|AAF91181.1|AF207903_2 (AF207903) bifunctional tryptophan synthase TRPB [Aspergillus
nidulans]
Length = 723
Score = 148 bits (369), Expect = 8e-35
Identities = 90/247 (36%), Positives = 137/247 (55%), Gaps = 7/247 (2%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGF 63
+L+ Y+TAG P+ + ++ LL +E GA IELG+PF+DPIADG TIQ+++ +AL G
Sbjct: 18 ALVAYITAGYPTIEESVDILLGLEN-GGADIIELGVPFTDPIADGPTIQKANTKALANGV 76
Query: 64 KLEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHA 122
+ +V+ R PI+LM YYNP+ G + K+ GV+G ++VDLP A
Sbjct: 77 TVSTVLEMVQHQRNRGLKAPIMLMGYYNPMLSYGEERMLRDCKEVGVNGFIMVDLPPEEA 136
Query: 123 SEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNL 182
F ++ G+ V L AP T + R+K + K + F+Y++S G TGA + L
Sbjct: 137 VRFRDLCTSTGLSYVPLIAPATSESRMKLLCKIADSFIYVVSRMGVTGATGTLSSNIPEL 196
Query: 183 LKRAKRICKN-KVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE-HGENAEEKLRE 240
L R + N A+GFGVS REH + + + GVV+GS +I ++ E A +K E
Sbjct: 197 LARVHKWSGNVPTALGFGVSTREHF-LSVQEISEGVVIGSQIITVLGEAPAGQAAQKAEE 255
Query: 241 KVRELAG 247
+ + G
Sbjct: 256 YLSSVTG 262
>sp|P42389|TRPA_BUCAP TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|625651|pir||D49897 tryptophan
synthase (EC 4.2.1.20) alpha chain - Buchnera aphidicola
>gi|396659|emb|CAA79501.1| (Z19055) alpha subunit of
tryptophan synthetase [Buchnera aphidicola]
Length = 271
Score = 148 bits (369), Expect = 8e-35
Identities = 78/243 (32%), Positives = 137/243 (56%), Gaps = 3/243 (1%)
Query: 3 RDGSLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKG 61
++G +P++ GDPS + +L+ + IE + A+ELGIPFSDP+ADG IQ++ RAL
Sbjct: 15 KEGCFVPFVVIGDPSLEMSLKIIETLIENGADALELGIPFSDPLADGPIIQKASLRALYK 74
Query: 62 GFKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
F ++ R K+ +PI ++ Y N V+ G++ F + SG+D +LI D+P+
Sbjct: 75 KNTFLQCFELIENIRNKYLHLPIGILLYANLVYNYGIKNFYYQCFRSGLDSVLIADIPIE 134
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
F ++A + I ++F+ P+ D L +I + G++YL+S G TG K +
Sbjct: 135 EYEPFYKIANQYNINSIFICPPDADDSLLSKISLYAKGYIYLLSRPGVTGIEKKTISLSN 194
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
+++ K+ + GFG+ ++ + +G NGV+ GSA+I+II E N E+K+
Sbjct: 195 IFIEKIKKYNSLPLLQGFGIENPTQIQKSILSGTNGVICGSAIIDII-EKNLNKEKKMIN 253
Query: 241 KVR 243
+++
Sbjct: 254 EIK 256
>sp|O68816|TRPA_STRCO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7481831|pir||T35065 tryptophan
synthase alpha chain - Streptomyces coelicolor
>gi|3169558|gb|AAC63503.1| (AF054585) tryptophan
synthase alpha subunit [Streptomyces coelicolor A3(2)]
>gi|5596781|emb|CAB51428.1| (AL096884) tryptophan
synthase alpha subunit [Streptomyces coelicolor A3(2)]
Length = 271
Score = 146 bits (366), Expect = 2e-34
Identities = 86/242 (35%), Positives = 135/242 (55%), Gaps = 4/242 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFL-LAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LI YL AG P+ + + A++ + +E+G+P SDP+ DG IQ + AL+GG +
Sbjct: 22 ALIAYLPAGFPTVTGGIEAVKAALDGGADVVEVGLPHSDPVLDGPVIQTADDIALRGGVR 81
Query: 65 LEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
+ VRE + + PI++MTY+NP+ R G+ F + ++G G ++ DLPV ++
Sbjct: 82 IADVMRTVREAHEATGKPILVMTYWNPIDRYGVERFTAELAEAGGAGCILPDLPVQESAL 141
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
+ E A + G+ TVF+ AP++ D RL EI +GFVY SL G TG R + A +L++
Sbjct: 142 WREHADKHGLATVFVVAPSSRDARLAEITAVGSGFVYAASLMGVTGTRASVGAQAEDLVR 201
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKVRE 244
R + V VG GVS + A+GV+VGSA + + + ++A E VR
Sbjct: 202 RTRATTDTPVCVGLGVSNAAQAAEVAGF-ADGVIVGSAFVKRMLDAPDDAAG--LEGVRA 258
Query: 245 LA 246
LA
Sbjct: 259 LA 260
>sp|O13831|TRP_SCHPO TRYPTOPHAN SYNTHASE >gi|7493535|pir||T37946 tryptophan synthase (EC
4.2.1.20) - fission yeast (Schizosaccharomyces pombe)
>gi|2388914|emb|CAB11651.1| (Z98974) tryptophan synthase
(EC 4.2.1.20) [Schizosaccharomyces pombe]
Length = 697
Score = 145 bits (361), Expect = 7e-34
Identities = 90/235 (38%), Positives = 136/235 (57%), Gaps = 8/235 (3%)
Query: 7 LIPYLTAGDPSAKATLRFLLAIEEY-SGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
L+ ++T G P+ T++ + ++ +G IELGIPFSD +ADG TI + + ALK L
Sbjct: 20 LVTFVTCGFPNVDETIKIMQGLQNGGAGIIELGIPFSDAVADGPTICKGNEIALKNNITL 79
Query: 66 EHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
E F V+ R +PI+LM YYNP+F G + I KAK+ G +G +IVDLP A
Sbjct: 80 EKVFETVKLARDAGVTIPIILMGYYNPIFSYGDAKTIQKAKEVGANGFIIVDLPPEEAVG 139
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTG--ARDKIPETAFNL 182
F E +++G+ V L AP+T D R++ + + F+Y++S G+TG A I L
Sbjct: 140 FREECKKQGVSFVPLVAPSTTDRRMELLASVADSFIYVVSRMGSTGSSATGVINTALPQL 199
Query: 183 LKRAKRIC-KNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINII--AEHGENA 234
+R ++ +AVGFGV+ EH + + ++GVVVGS +I++I AE G A
Sbjct: 200 CQRVRKFAGDTPLAVGFGVNTSEHFHQ-VGSVSDGVVVGSKIIDLILKAEPGTAA 253
>pir||D82688 tryptophan synthase alpha chain XF1376 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9106381|gb|AAF84185.1|AE003969_2 (AE003969)
tryptophan synthase alpha chain [Xylella fastidiosa]
Length = 268
Score = 144 bits (360), Expect = 9e-34
Identities = 86/226 (38%), Positives = 127/226 (56%), Gaps = 3/226 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+LIP++TAGDPS +A + + A + + IELG+PFSDP+ADG IQ S RAL+ G
Sbjct: 18 ALIPFITAGDPSLEAAVPVMHALVRAGADVIELGVPFSDPMADGPVIQHSSERALQRGVG 77
Query: 65 LEHAFNIVREFRKHSDV-PIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L + V FR+ V P+VLM Y NP+ G+ F A GVDG+L+VDLP A
Sbjct: 78 LAYVLQTVDVFRQSDAVTPVVLMGYLNPLEIYGIARFTQHAVACGVDGVLLVDLPPEEAD 137
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
E + G+ + LA+P T RL + + G++Y +S G TGA ++A + L
Sbjct: 138 EIRPIFSAAGLALIVLASPTTSASRLARLSGVAQGYLYYVSFSGVTGADRLDAQSAGDRL 197
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE 229
+ + + V VGFG+ ++ A+GVVVGSAL+ +++
Sbjct: 198 RGLRAQTQVPVVVGFGIRDAASAAVMA-VDADGVVVGSALVTALSD 242
>pir||S41995 tryptophan synthase (EC 4.2.1.20) alpha chain - red alga
(Cyanidium caldarium) chloroplast
>gi|742477|prf||2010216A Trp synthase:SUBUNIT=alpha
[Cyanidium caldarium]
Length = 242
Score = 143 bits (356), Expect = 3e-33
Identities = 70/225 (31%), Positives = 130/225 (57%), Gaps = 3/225 (1%)
Query: 8 IPYLTAGDPSAKATLRFLLAI-EEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I YLTAG P T + LL + + + IE+G+P+SDP+ADG +Q++ +ALK GF+LE
Sbjct: 3 IAYLTAGAPDLNTTKQALLNLANDGADVIEIGVPYSDPLADGMILQKASQQALKNGFRLE 62
Query: 67 HAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEFL 126
++++ E VP+V++ YYN +F G+ +++ + V +++ DLP A+
Sbjct: 63 QLWHLLAEIEL--PVPVVILAYYNQIFHYGVEKWVTTLVNLKVKALIVPDLPYEEAAILR 120
Query: 127 EVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKRA 186
+ ++L +P TP R K++ A ++YL+S G TG N++
Sbjct: 121 AACARHHLHMIWLISPTTPKVRAKQLALACDDWIYLVSRTGVTGVDAHFDNQIPNMIAEL 180
Query: 187 KRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHG 231
K++ +A+GFG+ +++ ++++ + GA+GV++G+A + I+ E G
Sbjct: 181 KQVTTTPIALGFGIHQKQQLQLVKSWGADGVIIGTACMQILLEKG 225
>sp|P43759|TRPA_HAEIN TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|1075399|pir||A64123 tryptophan
synthase (EC 4.2.1.20) alpha chain - Haemophilus
influenzae (strain Rd KW20) >gi|1574270|gb|AAC23079.1|
(U32822) tryptophan synthase alpha subunit (trpA)
[Haemophilus influenzae Rd]
Length = 268
Score = 142 bits (355), Expect = 3e-33
Identities = 74/240 (30%), Positives = 137/240 (56%), Gaps = 3/240 (1%)
Query: 4 DGSLIPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGG 62
+G+ +P++T DP+ + + ++ + A+ELG PFSDP+ DG IQ ++ RAL G
Sbjct: 16 EGAFVPFVTLCDPTFDRSFEIICTLVDNGADALELGFPFSDPLLDGPVIQAANNRALTAG 75
Query: 63 FKLEHAFNIVREFR-KHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMH 121
E + ++ + R K+ ++PI L+ N +F GL F + + GVD +L+ D+P++
Sbjct: 76 HSSEDSLKLLEKVRSKYPEIPISLLLCANLIFAKGLDAFYQRCAEVGVDAVLVADIPLLA 135
Query: 122 ASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFN 181
++++ A++ GI+ VF+ PN ++ ++ + K S G+ YL+S G T A ++ +
Sbjct: 136 KGDYVQTAKKHGIQPVFICPPNADEKTIQGVAKNSEGYTYLVSRAGVTSAENQAHAANLD 195
Query: 182 -LLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLRE 240
L+++ K + GFG+++ E V+ L+ G G + GSA + II + +N E+ L E
Sbjct: 196 TLVEKLKAHNAPPILQGFGIAQPEQVKEALSLGTAGAISGSATVKIIERNLDNHEQCLAE 255
>dbj|BAA04617.1| (D17791) Tryptophan synthase alpha subunit [Cyanidium caldarium]
Length = 242
Score = 140 bits (350), Expect = 1e-32
Identities = 69/225 (30%), Positives = 129/225 (56%), Gaps = 3/225 (1%)
Query: 8 IPYLTAGDPSAKATLRFLLAI-EEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I YLTAG P T + LL + + + IE+G+P+SDP+ADG +Q++ +ALK GF+LE
Sbjct: 3 IAYLTAGAPDLNTTKQALLNLANDGADVIEIGVPYSDPLADGMILQKASQQALKNGFRLE 62
Query: 67 HAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEFL 126
++++ E VP+V++ YYN +F G+ +++ + V +++ DLP A+
Sbjct: 63 QLWHLLAEIEL--PVPVVILAYYNQIFHYGVEKWVTTLVNLKVKALIVPDLPYEEAAILR 120
Query: 127 EVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKRA 186
+ ++L +P P R K++ A ++YL+S G TG N++
Sbjct: 121 AACARHHLHMIWLISPTLPKVRAKQLALACDDWIYLVSRTGVTGVDAHFDNQIPNMIAEL 180
Query: 187 KRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHG 231
K++ +A+GFG+ +++ ++++ + GA+GV++G+A + I+ E G
Sbjct: 181 KQVTTTPIALGFGIHQKQQLQLVKSWGADGVIIGTACMQILLEKG 225
>sp|P50908|TRPA_THEMA TRYPTOPHAN SYNTHASE ALPHA CHAIN
>gi|4980629|gb|AAD35230.1|AE001699_7 (AE001699)
tryptophan synthase, alpha subunit [Thermotoga maritima]
Length = 239
Score = 140 bits (350), Expect = 1e-32
Identities = 89/244 (36%), Positives = 136/244 (55%), Gaps = 14/244 (5%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEYS-GAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I Y+ AG P + T + L+A+ E +E+G+PFSDP+ADG IQ +H AL+ G ++
Sbjct: 5 IAYIPAGFPDLETTRKILIALNELGITGVEIGVPFSDPVADGPVIQLAHSVALRNGVTIK 64
Query: 67 HAFNIVREFRKHSDVPIVLMTYYNPV--FRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
++ E D + LM+Y NP+ + G + + + K GV G++I DLP+
Sbjct: 65 KILEMLSEI--SVDYDLYLMSYLNPIVNYPEGKEKLLDELKKLGVKGLIIPDLPLREVKN 122
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
+++A V APNT DE + I+ FVY IS YG TG R+ +P + +K
Sbjct: 123 -VDIA----YPIVPFVAPNTKDEEIDLINSVQAPFVYYISRYGVTGEREDLPFA--DHIK 175
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEH-GENAEEKLREKVR 243
R K K + VGFG+S+ E V+ + A+GV+VGSAL+ I+ E+ + K+ EKV+
Sbjct: 176 RVKERIKLPLFVGFGISRHEQVKKVWEI-ADGVIVGSALVRIMEENPKDEIPRKVVEKVK 234
Query: 244 ELAG 247
EL G
Sbjct: 235 ELLG 238
>sp|P34793|TRPA_CYACA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7484298|pir||T14365 probable
tryptophan synthase (EC 4.2.1.20) alpha chain - red alga
(Cyanidium caldarium) chloroplast
>gi|2541888|dbj|BAA22823.1| (D63676) tryptophan synthase
alpha subunit [Cyanidium caldarium]
Length = 242
Score = 139 bits (348), Expect = 2e-32
Identities = 69/225 (30%), Positives = 129/225 (56%), Gaps = 3/225 (1%)
Query: 8 IPYLTAGDPSAKATLRFLLAI-EEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I YLTAG P T + LL + + + IE+G+P+SDP+ADG +Q++ +ALK F+LE
Sbjct: 3 IAYLTAGAPDLNTTKQALLNLANDGADVIEIGVPYSDPLADGMILQKASQQALKNRFRLE 62
Query: 67 HAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEFL 126
++++ E VP+V++ YYN +F G+ +++ + V +++ DLP A+
Sbjct: 63 QLWHLLAEIEL--PVPVVILAYYNQIFHYGVEKWVTTLVNLKVKALIVPDLPYEEAAILR 120
Query: 127 EVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKRA 186
+ ++L +P TP R K++ A ++YL+S G TG N++
Sbjct: 121 AACARHHLHMIWLISPTTPKVRAKQLALACDDWIYLVSRTGVTGVDAHFDNQIPNMIAEL 180
Query: 187 KRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHG 231
K++ +A+GFG+ +++ ++++ + GA+GV++G+A + I+ E G
Sbjct: 181 KQVTTTPIALGFGIHQKQQLQLVKSWGADGVIIGTACMQILLEKG 225
>pir||S59050 tryptophan synthase alpha chain trpA - Thermotoga maritima (strain
MSB8) >gi|1054862|emb|CAA63392.1| (X92729) tryptophan
synthase alpha-subunit [Thermotoga maritima]
Length = 239
Score = 138 bits (344), Expect = 6e-32
Identities = 88/244 (36%), Positives = 135/244 (55%), Gaps = 14/244 (5%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEYS-GAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I Y+ AG P + T + L+A+ E +E+G+PFSDP+ADG IQ +H AL+ G ++
Sbjct: 5 IAYIPAGFPDLETTRKILIALNELGITGVEIGVPFSDPVADGPVIQLAHSVALRNGVTIK 64
Query: 67 HAFNIVREFRKHSDVPIVLMTYYNPV--FRVGLREFIGKAKDSGVDGMLIVDLPVMHASE 124
++ E D + LM+Y NP+ + G + + + K GV G++I DLP+
Sbjct: 65 KILEMLSEI--SVDYDLYLMSYLNPIVNYPEGKEKLLDELKKLGVKGLIIPDLPLREVKN 122
Query: 125 FLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLK 184
+++A V APNT DE + I+ FVY IS YG TG R+ +P + +K
Sbjct: 123 -VDIA----YPIVPFVAPNTKDEEIDLINSVQAPFVYYISRYGVTGEREDLPFA--DHIK 175
Query: 185 RAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEH-GENAEEKLREKVR 243
R K K + VG G+S+ E V+ + A+GV+VGSAL+ I+ E+ + K+ EKV+
Sbjct: 176 RVKERIKLPLFVGLGISRHEQVKKVWEI-ADGVIVGSALVRIMEENPKDEIPRKVVEKVK 234
Query: 244 ELAG 247
EL G
Sbjct: 235 ELLG 238
>gi|11465459 unknown; tryptophan synthase alpha subunit [Cyanidium caldarium]
>gi|6466362|gb|AAF12944.1|AF022186_67 (AF022186)
unknown; tryptophan synthase alpha subunit [Cyanidium
caldarium]
Length = 266
Score = 138 bits (344), Expect = 6e-32
Identities = 68/224 (30%), Positives = 125/224 (55%), Gaps = 1/224 (0%)
Query: 7 LIPYLTAGDPSAKATLRFLLAIEEY-SGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
L+P+++ G P+ + + ++A+++ + IELGIP+SDP+ADG IQ ++ +A+K G +
Sbjct: 17 LLPFVSLGTPNTQINKQAIIAMDKNGANIIELGIPYSDPVADGPVIQDAYNKAIKNGVNI 76
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
AF I+ + PI++ YYN + G+ +F+ K V G+++ DLP +
Sbjct: 77 RKAFKILMNLKGKIKSPIIVFIYYNQLLNYGINKFLEKLIQLEVQGIIVPDLPYDESQIL 136
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKR 185
+ I + L A + R+K+I + + GF+YLIS G TG K+ ++K
Sbjct: 137 KKKCTINNIALISLIALTSSFSRIKKIARNAEGFLYLISKTGVTGGTGKLMNKLKIIIKT 196
Query: 186 AKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE 229
+++ V VGFG++ R ++ L+ +NG+V+GS + I+ +
Sbjct: 197 IQKLTSKPVVVGFGINSRRQIKQLIEWNSNGIVIGSPCVQILLQ 240
>sp|O68906|TRPA_MYCIT TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|3152734|gb|AAC17135.1|
(AF057042) tryptophan synthase alpha subunit
[Mycobacterium intracellulare]
Length = 271
Score = 130 bits (324), Expect = 1e-29
Identities = 84/239 (35%), Positives = 124/239 (51%), Gaps = 5/239 (2%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGF 63
+LI YL G P ++ ++A+ E SG +E+G+P+SDP DG TI ++ AL GG
Sbjct: 25 ALIGYLPTGYPDVTTSVHAMVALVE-SGCDIVEVGVPYSDPGMDGPTIVKATEAALHGGV 83
Query: 64 KLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
++ V +V MTY+NPV R G+ F +G G++ DL A
Sbjct: 84 RVRDTLAAVEAISAAGGHAVV-MTYWNPVLRYGIDAFARDLASAGGYGLITPDLIPDEAG 142
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
++L + E + +FL AP++ +RL +AS GFVY S G TGARD + A L+
Sbjct: 143 QWLAASEEHRLDRIFLVAPSSTPQRLAMTVEASRGFVYAASTMGVTGARDVVSNAAPELV 202
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKV 242
R K I V VG GV E + A+GV+VGSAL++ + + G A L E++
Sbjct: 203 GRVKEISDIPVGVGLGVRSGEQAAQ-IGGYADGVIVGSALVSALGDGGLTALRALTEEL 260
>sp|P16578|TRP_COPCI TRYPTOPHAN SYNTHASE >gi|83890|pir||JU0401 tryptophan synthase (EC
4.2.1.20) - inky cap (Coprinus cinereus)
>gi|167217|gb|AAA51432.1| (M91661) tryptophan synthetase
[Coprinus cinereus]
Length = 702
Score = 129 bits (322), Expect = 2e-29
Identities = 87/239 (36%), Positives = 130/239 (53%), Gaps = 8/239 (3%)
Query: 16 PSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGFKLEHAFNIVR 73
P + T+ LLA++ GA IELGIPFSDPIADG IQ+++ ALK +R
Sbjct: 25 PKKEDTVPVLLALQA-GGADIIELGIPFSDPIADGPVIQEANTVALKNDIDYPTVLGQIR 83
Query: 74 EFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEFLEVAREE 132
E R+ P++LM YYNP+ G + I A ++G +G ++VDLP A F +
Sbjct: 84 EARQQGLTAPVLLMGYYNPMLAYGEDKAIQDAAEAGANGFIMVDLPPEEAIAFRQKCAAS 143
Query: 133 GIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTG--ARDKIPETAFNLLKRAKRIC 190
+ V L AP+T +R++ + + F+Y++S GTTG A + E +L R +
Sbjct: 144 NLSYVPLIAPSTTLKRIQFLASIADSFIYVVSKMGTTGSSANVAVNEELPTILSRIREYT 203
Query: 191 KNKVAVGFGVSKREHVEMLLNAGANGVVV-GSALINIIAEHGENAEEKLREK-VRELAG 247
+AV FGV+ R+ + +AGA+GVVV GS ++N I GE + E RE++G
Sbjct: 204 HVPLAVEFGVATRDQFNYVADAGADGVVVIGSRIVNAIKAAGEGQVPQFVENYCREVSG 262
>sp|O06130|TRPA_MYCTU TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436996|pir||C70557 probable
trpA protein - Mycobacterium tuberculosis (strain H37RV)
>gi|2113897|emb|CAB08890.1| (Z95554) trpA [Mycobacterium
tuberculosis]
Length = 270
Score = 129 bits (322), Expect = 2e-29
Identities = 84/226 (37%), Positives = 121/226 (53%), Gaps = 5/226 (2%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSGA--IELGIPFSDPIADGKTIQQSHFRALKGGF 63
+LI YL G P A++ + A+ E SG IE+G+P+SDP DG TI ++ AL+GG
Sbjct: 26 ALIGYLPTGYPDVPASVAAMTALVE-SGCDIIEVGVPYSDPGMDGPTIARATEAALRGGV 84
Query: 64 KLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
++ V E + V+MTY+NPV R G+ F +G G++ DL A
Sbjct: 85 RVRDTLAAV-EAISIAGGRAVVMTYWNPVLRYGVDAFARDLAAAGGLGLITPDLIPDEAQ 143
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLL 183
++L + E + +FL AP++ ERL +AS GFVY S G TGARD + + A L+
Sbjct: 144 QWLAASEEHRLDRIFLVAPSSTPERLAATVEASRGFVYAASTMGVTGARDAVSQAAPELV 203
Query: 184 KRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE 229
R K + V VG GV R + A+GV+VGSAL+ + E
Sbjct: 204 GRVKAVSDIPVGVGLGVRSRAQAAQIAQY-ADGVIVGSALVTALTE 248
>sp|Q01997|TRPA_LACLA TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|421429|pir||S35130 tryptophan
synthase (EC 4.2.1.20) alpha chain - Lactococcus lactis
subsp. lactis >gi|149522|gb|AAA25229.1| (M87483)
tryptophan synthase alpha subunit [Lactococcus lactis]
Length = 253
Score = 128 bits (319), Expect = 6e-29
Identities = 79/243 (32%), Positives = 124/243 (50%), Gaps = 10/243 (4%)
Query: 3 RDGSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFR 57
+ + IPY+ AGD K T++ L + S AIE+G+PFSDP+ADG I+Q+ R
Sbjct: 11 KKNNFIPYIMAGDHEKGLEGLKETIQLLE--QAGSSAIEIGVPFSDPVADGPVIEQAGLR 68
Query: 58 ALKGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDL 117
AL L ++ + VP+V+MTY+NPV++ G+ +F+ + + V G++I DL
Sbjct: 69 ALARNVSLSSILETLKTI--DTKVPLVIMTYFNPVYQFGIEKFVAALEKTPVKGLIIPDL 126
Query: 118 PVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPE 177
P H ++ I V L + TP R KE+ + GF+Y +++ G TG +
Sbjct: 127 PKEHEDYIKPFINDKDICLVPLVSLTTPLSRQKELVADAEGFIYAVAINGVTGKENAYSN 186
Query: 178 TAFNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEK 237
LK + V GFG+S V+ N ++GV+VGS ++ + E EN K
Sbjct: 187 QLDQHLKALSSLTDVPVLTGFGISTLSDVDR-FNKVSSGVIVGSKIVRDLHEGKENEVIK 245
Query: 238 LRE 240
E
Sbjct: 246 FIE 248
>pir||PN0500 tryptophan synthase (EC 4.2.1.20) alpha chain - Bacillus subtilis
(fragment)
Length = 180
Score = 118 bits (292), Expect = 8e-26
Identities = 59/169 (34%), Positives = 103/169 (60%), Gaps = 3/169 (1%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEY-SGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
IP++TAGDP + ++ +++ + A+E+G+P++DP+ADG IQ++ RAL+ G +
Sbjct: 12 IPFITAGDPLPEISVELAKSLQNAGASALEIGVPYTDPLADGPVIQRASKRALENGMNIV 71
Query: 67 HAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
A + + +K+ +PI+L TYYNPV + F +++ +DG+L+ DLP+ ++
Sbjct: 72 KAIELGGKMKKNGVHIPIILFTYYNPVLELEKECFFALLRENDIDGLLVPDLPLEESALL 131
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDK 174
+ ++E I + L AP T + RLK I + + GFVY +S G TG R +
Sbjct: 132 QKTCKKENIAYISLVAP-TSENRLKIITEQAGGFVYCVSSLGVTGVRSE 179
>gb|AAC60451.1| (S65045) trpA [Bacillus subtilis, K, Peptide Partial, 180 aa]
Length = 180
Score = 117 bits (291), Expect = 1e-25
Identities = 59/169 (34%), Positives = 103/169 (60%), Gaps = 3/169 (1%)
Query: 8 IPYLTAGDPSAKATLRFLLAIEEY-SGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
IP++TAGDP + ++ ++E + A+E+G+P++DP+ADG I+++ RAL+ G +
Sbjct: 12 IPFITAGDPLPEISVELAKSLENAGASALEIGVPYTDPLADGPVIERASKRALENGMNIV 71
Query: 67 HAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
A + + +K+ +PI+L TYYNPV + F +++ +DG+L+ DLP+ ++
Sbjct: 72 KAIELGGKMKKNGVHIPIILFTYYNPVLELEKECFFALLRENDIDGLLVPDLPLEESALL 131
Query: 126 LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDK 174
+ ++E I + L AP T + RLK I + + GFVY +S G TG R +
Sbjct: 132 EKTCKKENIAYISLVAP-TSENRLKIITEQAGGFVYCVSSLGVTGVRSE 179
>gb|AAD41125.1|AF157826_2 (AF157826) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 115 bits (284), Expect = 7e-25
Identities = 69/208 (33%), Positives = 108/208 (51%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLAETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ ++VP+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEVPLVIMTYFNPLFQYGVENFVKDLVDTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLADTDIALIPLVSLTTGLERQKELIEGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + VE
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADVE 221
>gb|AAD41117.1|AF157822_2 (AF157822) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 114 bits (283), Expect = 9e-25
Identities = 67/208 (32%), Positives = 109/208 (52%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLGETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ + +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIK--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>gb|AAD41123.1|AF157825_2 (AF157825) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 114 bits (283), Expect = 9e-25
Identities = 67/208 (32%), Positives = 109/208 (52%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLGETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ + +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIK--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLADTDITLIPLVSLTTGIERQKELIEGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>gb|AAD41109.1|AF157818_2 (AF157818) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 114 bits (282), Expect = 1e-24
Identities = 67/208 (32%), Positives = 108/208 (51%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLAETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLADTDITLIPLVSLTTGIERQKELIEGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>gb|AAD41113.1|AF157820_2 (AF157820) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 114 bits (282), Expect = 1e-24
Identities = 67/208 (32%), Positives = 108/208 (51%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLAETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>gb|AAD41107.1|AF157817_2 (AF157817) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 113 bits (281), Expect = 2e-24
Identities = 66/208 (31%), Positives = 108/208 (51%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLAETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GF+Y +++ G TG
Sbjct: 134 EHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFIYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>gb|AAD41115.1|AF157821_2 (AF157821) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 113 bits (281), Expect = 2e-24
Identities = 67/208 (32%), Positives = 108/208 (51%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLAETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQVDLE 221
>gb|AAD41121.1|AF157824_2 (AF157824) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 113 bits (281), Expect = 2e-24
Identities = 66/208 (31%), Positives = 108/208 (51%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLAETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GF+Y +++ G TG
Sbjct: 134 EHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFIYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>gb|AAD41111.1|AF157819_2 (AF157819) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 112 bits (278), Expect = 4e-24
Identities = 67/208 (32%), Positives = 106/208 (50%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLAETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ +++P+V+MTY+NP+F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ I + L + T ER KE+ + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLANTDIALIPLVSLTTGIERQKELIAGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>pir||D81377 tryptophan synthase (EC 4.2.1.20) alpha chain Cj0349 [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6967824|emb|CAB74186.1| (AL139075) tryptophan
synthase alpha chain [Campylobacter jejuni]
Length = 249
Score = 112 bits (277), Expect = 5e-24
Identities = 60/224 (26%), Positives = 117/224 (51%), Gaps = 4/224 (1%)
Query: 2 FRDGSLIPYLTAGDPSAKATLRFLLAIEEYS-GAIELGIPFSDPIADGKTIQQSHFRALK 60
+++ + + Y G P+ + + FL +++ +ELG+ +SDPIADG+ I + AL
Sbjct: 8 YKENANVAYTVLGYPNLQTSEAFLQRLDQSPIDILELGVAYSDPIADGEIIADAAKIALD 67
Query: 61 GGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM 120
G + F ++ + +V M YYN +F GL +F+ KAK G+ +++ +L
Sbjct: 68 QGVDIHSVFELLARIKTKK--ALVFMVYYNLIFSYGLEKFVKKAKSLGICALIVPELSFE 125
Query: 121 HASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAF 180
+ + ++ I + L + TP ER+K++ K + GF+YL++ G TG +
Sbjct: 126 ESDDLIKECERYNIALITLVSVTTPKERVKKLVKHAKGFIYLLASIGITGTKSVEEAILQ 185
Query: 181 NLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALI 224
+ +K + + VGFG+ + V+ + A+GV+VG++++
Sbjct: 186 DKVKEIRSFTNLPIFVGFGIQNNQDVKRMRKV-ADGVIVGTSIV 228
>gb|AAD41119.1|AF157823_2 (AF157823) tryptophan synthase alpha chain [Streptococcus
pneumoniae]
Length = 224
Score = 110 bits (273), Expect = 1e-23
Identities = 66/208 (31%), Positives = 107/208 (50%), Gaps = 9/208 (4%)
Query: 5 GSLIPYLTAGD-----PSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRAL 59
G +PY+ AGD T+ FL + AIE+GIPFSDP+ADG I+++ R+L
Sbjct: 18 GIFVPYIMAGDHEKGLDGLGETIHFLEDLGV--SAIEVGIPFSDPVADGPVIEEAGLRSL 75
Query: 60 KGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPV 119
G + ++ +++P+V+MTY+N +F+ G+ F+ D+ V G++I DLP
Sbjct: 76 AHGTSTQALVETLKTIE--TEIPLVIMTYFNSLFQYGVENFVKDLADTAVKGLIIPDLPH 133
Query: 120 MHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETA 179
HA+ + I + L + T ER KE+ + + GFVY +++ G TG
Sbjct: 134 EHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFVYAVAINGVTGKSGNYRADL 193
Query: 180 FNLLKRAKRICKNKVAVGFGVSKREHVE 207
L + ++ V GFGVS + +E
Sbjct: 194 DKHLAQLHQVADIPVLTGFGVSSQADLE 221
>emb|CAA24636.1| (V01343) put. tryptophan synthetase [Saccharomyces cerevisiae]
Length = 173
Score = 109 bits (271), Expect = 2e-23
Identities = 61/147 (41%), Positives = 85/147 (57%), Gaps = 2/147 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYS-GAIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ ++TAG P+ K T+ L ++ IELG+PFSDPIADG TIQ S+ AL+ G
Sbjct: 19 ALVTFMTAGYPTVKDTVPILKGFQDGGVDIIELGMPFSDPIADGPTIQLSNTVALQNGVT 78
Query: 65 LEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHAS 123
L +V + R VPI+LM YYNP+ G FI A +G +G +IVDLP A
Sbjct: 79 LPQTLEMVSQARNEGVTVPIILMGYYNPILNYGEERFIQDAAKAGANGFIIVDLPPEEAL 138
Query: 124 EFLEVAREEGIKTVFLAAPNTPDERLK 150
+ + G+ + L AP+T DERL+
Sbjct: 139 KVRNYINDNGLSLIPLVAPSTTDERLE 165
>gb|AAG18888.1| (AE004991) tryptophan synthase alpha chain; TrpA [Halobacterium sp.
NRC-1]
Length = 204
Score = 109 bits (270), Expect = 3e-23
Identities = 64/177 (36%), Positives = 101/177 (56%), Gaps = 7/177 (3%)
Query: 76 RKHSDVPIVLMTYYNPVFRVGLRE----FIGKAKDSGVDGMLIVDLPVMHASEFLEVARE 131
R +DVP+V MTYYN +F+ G R F+ A ++GV G ++ DLPV + E R
Sbjct: 12 RIDADVPVVCMTYYNLLFQYGDRAGPAAFVSAAAEAGVSGFVVPDLPVDESGPLREACRA 71
Query: 132 EGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKRAKRICK 191
G+ VF+ AP T +R + + +TGFVY+ GTTGAR+++ + L +
Sbjct: 72 HGLDLVFVVAPTTTADRRERMLDLTTGFVYVQGRVGTTGAREEVSAATPDALAALQHTDI 131
Query: 192 NKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAE--HGENAEEKLREKVRELA 246
K AVGFGVS E + +GA+GV+VGSA ++ +A+ ++ + +++R+LA
Sbjct: 132 PK-AVGFGVSSGEQAREITASGADGVIVGSAYVDTVADGVADDDPPSVVADRLRDLA 187
>gb|AAK41172.1| Tryptophan synthase alpha chain (trpA) [Sulfolobus solfataricus]
Length = 245
Score = 104 bits (256), Expect = 1e-21
Identities = 70/243 (28%), Positives = 126/243 (51%), Gaps = 13/243 (5%)
Query: 7 LIPYLTAGDPSAKATLRFLL-AIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
L+ Y+T G P+ ++ F++ A+E + +ELGIP DG I++S+ + K
Sbjct: 7 LVVYMTLGYPNVQSFKDFIIGAVENGADILELGIPPKYAKYDGPVIRKSYDKV-----KG 61
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
+ ++ + RK VPI+ +TY + L F+ KD +DG+L DL + + +
Sbjct: 62 LDIWPLIEDIRKDVGVPIIALTYLED-WVDQLENFLNMIKDVKLDGILFPDLLIDYIDDL 120
Query: 126 LEV---AREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNL 182
++ + +G+K V +P+ PD + ++ K S F+Y + TTG IP + L
Sbjct: 121 DKIDGIIKNKGLKNVIFTSPSVPDLLIHKVSKISDLFLYY-GVRPTTGV--PIPVSVKQL 177
Query: 183 LKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKV 242
+ R + + +NK+ VGFG+S + L+AGA+G+ +G+ I I +G + L +K
Sbjct: 178 INRVRNLVENKLIVGFGLSSESDLRDALSAGADGIAIGTVFIEEIERNGVKSAINLVKKF 237
Query: 243 REL 245
R +
Sbjct: 238 RAI 240
>sp|P50382|TRPA_SULSO TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|1004320|emb|CAA90308.1|
(Z50014) tryptophan synthase component a [Sulfolobus
solfataricus]
Length = 243
Score = 104 bits (256), Expect = 1e-21
Identities = 70/243 (28%), Positives = 126/243 (51%), Gaps = 13/243 (5%)
Query: 7 LIPYLTAGDPSAKATLRFLL-AIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKL 65
L+ Y+T G P+ ++ F++ A+E + +ELGIP DG I++S+ + K
Sbjct: 5 LVVYMTLGYPNVQSFKDFIIGAVENGADILELGIPPKYAKYDGPVIRKSYDKV-----KG 59
Query: 66 EHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVMHASEF 125
+ ++ + RK VPI+ +TY + L F+ KD +DG+L DL + + +
Sbjct: 60 LDIWPLIEDIRKDVGVPIIALTYLED-WVDQLENFLNMIKDVKLDGILFPDLLIDYIDDL 118
Query: 126 LEV---AREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNL 182
++ + +G+K V +P+ PD + ++ K S F+Y + TTG IP + L
Sbjct: 119 DKIDGIIKNKGLKNVIFTSPSVPDLLIHKVSKISDLFLYY-GVRPTTGV--PIPVSVKQL 175
Query: 183 LKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIAEHGENAEEKLREKV 242
+ R + + +NK+ VGFG+S + L+AGA+G+ +G+ I I +G + L +K
Sbjct: 176 INRVRNLVENKLIVGFGLSSESDLRDALSAGADGIAIGTVFIEEIERNGVKSAINLVKKF 235
Query: 243 REL 245
R +
Sbjct: 236 RAI 238
>dbj|BAA96470.1| (AB036428) hypothetical tryptophan synthase alpha chain protein
[Streptococcus mutans]
Length = 202
Score = 98.7 bits (242), Expect = 6e-20
Identities = 63/187 (33%), Positives = 97/187 (51%), Gaps = 3/187 (1%)
Query: 43 DPIADGKTIQQSHFRALKGGFKLEHAFNIVREFRKHSDVPIVLMTYYNPVFRVGLREFIG 102
DP+ADG I+ + R+L G L A I R K + VP+V+MTY+NPVF+ G+ F+
Sbjct: 1 DPVADGPVIELAGQRSLVKGTSL--ASIIARLQEKKTQVPLVIMTYFNPVFQYGVETFVA 58
Query: 103 KAKDSGVDGMLIVDLPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYL 162
+++ V G++I DLP S + V L + T ER KE+ + + GFVY
Sbjct: 59 DLQNTSVKGLIIPDLPHEQESFIKPYLENLDLALVPLVSLTTGLERQKELIEDAQGFVYA 118
Query: 163 ISLYGTTGARDKIPETAFNLLKRAKRICKNKVAVGFGVSKREHVEMLLNAGANGVVVGSA 222
+++ G TG + LK I + V GFGVS ++ N ++GV+VGS
Sbjct: 119 VAINGVTGKTGNYRDDLDKHLKHLTEIAQIPVLTGFGVSTLADIKR-FNQVSDGVIVGSK 177
Query: 223 LINIIAE 229
++ + E
Sbjct: 178 IVKGLHE 184
>sp|O84173|TRPA_CHLTR TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436995|pir||B71547 probable
tryptophane synthase (alpha chain) - Chlamydia
trachomatis (serotype D, strain UW3/Cx)
>gi|3328575|gb|AAC67762.1| (AE001291) Tryptophan
Synthase (Alpha Chain) [Chlamydia trachomatis]
Length = 253
Score = 97.9 bits (240), Expect = 1e-19
Identities = 62/176 (35%), Positives = 91/176 (51%), Gaps = 7/176 (3%)
Query: 8 IPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I YLTAGD T+ A I+ +ELG PFSDP+AD IQ SH RAL E
Sbjct: 15 IGYLTAGDGGTSYTIEAAKALIQGGVDILELGFPFSDPVADNPEIQVSHDRALAENLTSE 74
Query: 67 HAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM----H 121
IV R + +VP++L +YYNP+ + L +++ + KD+G++G+ ++DLP
Sbjct: 75 TLLEIVEGIRAFNQEVPLILYSYYNPLLQRDL-DYLRRLKDAGINGVCVIDLPAPLSHGE 133
Query: 122 ASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPE 177
S F E G+ + L + T ER+ I + + GF+Y I T + I E
Sbjct: 134 KSPFFEDLLAVGLDPILLISAGTTPERMSLIQEYARGFLYYIPCQATRDSEVGIKE 189
>emb|CAB63649.1| (AJ249639) tryptophan synthase alpha chain [Chlamydia trachomatis]
Length = 183
Score = 90.5 bits (221), Expect = 2e-17
Identities = 57/161 (35%), Positives = 87/161 (53%), Gaps = 6/161 (3%)
Query: 8 IPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I YLTAGD T+ A I+ +ELG PFSDP+AD IQ SH RAL E
Sbjct: 15 IGYLTAGDGGTSYTIEAAKALIQGGVDILELGFPFSDPVADNPEIQVSHDRALAENLTSE 74
Query: 67 HAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDL--PVMHAS 123
IV R + +VP++L +YYNP+ + L +++ + KD+G++G+ ++DL P+ H
Sbjct: 75 TLLEIVEGIRAFNQEVPLILYSYYNPLLQRDL-DYLRRLKDAGINGVCVIDLPAPLSHGE 133
Query: 124 EF-LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLI 163
+ E G+ + L + T ER+ I + + G +Y I
Sbjct: 134 KSPFEDLLAVGLDPILLISAGTTPERMSLIQEHARGLLYYI 174
>emb|CAB63648.1| (AJ249638) tryptophan synthase alfa chain [Chlamydia trachomatis]
Length = 183
Score = 90.1 bits (220), Expect = 2e-17
Identities = 57/161 (35%), Positives = 87/161 (53%), Gaps = 6/161 (3%)
Query: 8 IPYLTAGDPSAKATLRFLLA-IEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLE 66
I YLTAGD T+ A I+ +ELG PFSDP+AD IQ SH RAL E
Sbjct: 15 IGYLTAGDGGTSYTIEAAKALIQGGVDILELGFPFSDPVADDPEIQVSHDRALAENLTSE 74
Query: 67 HAFNIVREFRK-HSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDL--PVMHAS 123
IV R + +VP++L +YYNP+ + L +++ + KD+G++G+ ++DL P+ H
Sbjct: 75 TLLEIVEGIRAFNQEVPLILYSYYNPLLQRDL-DYLRRLKDAGINGVCVIDLPAPLSHGE 133
Query: 124 EF-LEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLI 163
+ E G+ + L + T ER+ I + + G +Y I
Sbjct: 134 KSPFEDLLAVGLDPILLISAGTTPERMSLIQEHARGLLYYI 174
>gb|AAD04348.1| (AF084880) mutant tryptophan synthase [Neurospora crassa]
Length = 129
Score = 87.0 bits (212), Expect = 2e-16
Identities = 46/112 (41%), Positives = 70/112 (62%), Gaps = 2/112 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ Y+TAG P + T LLA+E+ I+LG+PF+DPIADG TIQ ++ AL+ G
Sbjct: 18 ALVTYVTAGFPHPEQTPDILLAMEKGGADVIKLGVPFTDPIADGPTIQTANTIALQHGVT 77
Query: 65 LEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIV 115
L+ +VR+ R+ P++LM YYNP+ G + K++GV+G +IV
Sbjct: 78 LQSTLQMVRDARQRGLKAPVMLMGYYNPLLSYGEERLLNDCKEAGVNGFIIV 129
>gb|AAD04350.1| (AF084882) mutant tryptophan synthase [Neurospora crassa]
>gi|4151269|gb|AAD04351.1| (AF084883) mutant tryptophan
synthase [Neurospora crassa] >gi|4151271|gb|AAD04352.1|
(AF084884) mutant tryptophan synthase [Neurospora
crassa] >gi|4151273|gb|AAD04353.1| (AF084885) mutant
tryptophan synthase [Neurospora crassa]
Length = 129
Score = 85.8 bits (209), Expect = 4e-16
Identities = 46/112 (41%), Positives = 69/112 (61%), Gaps = 2/112 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ Y+TAG P + T LLA+E+ IELG+PF+DPIAD TIQ ++ AL+ G
Sbjct: 18 ALVTYVTAGFPHPEQTPDILLAMEKGGADVIELGVPFTDPIADDPTIQTANTIALQHGVT 77
Query: 65 LEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIV 115
L+ +VR+ R+ P++LM YYNP+ G + K++GV+G +IV
Sbjct: 78 LQSTLQMVRDARQRGLKAPVMLMGYYNPLLSYGEERLLNDCKEAGVNGFIIV 129
>gb|AAD04349.1| (AF084881) mutant tryptophan synthase [Neurospora crassa]
Length = 129
Score = 85.0 bits (207), Expect = 7e-16
Identities = 46/112 (41%), Positives = 69/112 (61%), Gaps = 2/112 (1%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFRALKGGFK 64
+L+ Y+TAG P + T LLA+E+ IEL +PF+DPIADG TIQ ++ AL+ G
Sbjct: 18 ALVTYVTAGFPHPEQTPDILLAMEKGGADVIELVVPFTDPIADGPTIQTANTIALQHGVT 77
Query: 65 LEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIV 115
L+ +VR+ R+ P++LM YYNP+ G + K++GV+G +IV
Sbjct: 78 LQSTLQMVRDARQRGLKAPVMLMGYYNPLLSYGEERLLNDCKEAGVNGFIIV 129
>gb|AAD04354.1| (AF084886) mutant tryptophan synthase [Neurospora crassa]
Length = 131
Score = 83.5 bits (203), Expect = 2e-15
Identities = 47/114 (41%), Positives = 69/114 (60%), Gaps = 4/114 (3%)
Query: 6 SLIPYLTAGDPSAKATLRFLLAIEEYSG-AIELGIPFSDPIADGKTIQQSHFR--ALKGG 62
+L+ Y+TAG P + T LLA+E+ IELG+PF+DPIADG TIQ + AL+ G
Sbjct: 18 ALVTYVTAGFPHPEQTPDILLAMEKGGADVIELGVPFTDPIADGPTIQTATANTIALQHG 77
Query: 63 FKLEHAFNIVREFRKHS-DVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIV 115
L+ +VR+ R+ P++LM YYNP+ G + K++GV+G +IV
Sbjct: 78 VTLQSTLQMVRDARQRGLKAPVMLMGYYNPLLSYGEERLLNDCKEAGVNGFIIV 131
>gb|AAD04358.1| (AF084890) mutant tryptophan synthase [Neurospora crassa]
Length = 193
Score = 82.7 bits (201), Expect = 4e-15
Identities = 50/134 (37%), Positives = 74/134 (54%), Gaps = 2/134 (1%)
Query: 109 VDGMLIVDLPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGT 168
V+G +IVDLP A F ++ G+ V L AP T D R++ + + + F+Y++S G
Sbjct: 1 VNGFIIVDLPPEEAVSFRQLCTRGGLSYVPLIAPATSDARMRVLCQLADSFIYVVSRQGV 60
Query: 169 TGARDKIPETAFNLLKRAKRICKNK-VAVGFGVSKREHVEMLLNAGANGVVVGSALINII 227
TGA + LL R K+ NK AVGFGVS +H + A A+GVVVGS +I I
Sbjct: 61 TGASGTLNANLPELLARVKKYSGNKPAAVGFGVSTHDHFTQ-VGAIADGVVVGSMIITTI 119
Query: 228 AEHGENAEEKLREK 241
+ + E K +++
Sbjct: 120 QKAAKGEEVKAKQE 133
>sp|Q9Y8T3|TRPA_AERPE TRYPTOPHAN SYNTHASE ALPHA CHAIN >gi|7436993|pir||G72488 probable
tryptophan synthase alpha chain APE2550 - Aeropyrum
pernix (strain K1) >gi|5106256|dbj|BAA81567.1|
(AP000064) 252aa long hypothetical tryptophan synthase
alpha chain [Aeropyrum pernix]
Length = 252
Score = 81.1 bits (197), Expect = 1e-14
Identities = 61/223 (27%), Positives = 101/223 (44%), Gaps = 8/223 (3%)
Query: 10 YLTAGDPSAKATLRFLLAIEEYSGAIELGIPFSDPIADGKTIQQSHFRALKGGFKLEHAF 69
YLT PS L ++ +ELGIP P+ DG TI+ +H +A++ G+
Sbjct: 11 YLTIAWPSPDTFLEIASTLKGCVDYLELGIPTPKPLYDGPTIRLTHLKAVESGYSGPKTL 70
Query: 70 NIVREFRKHSDVPIVLMTYYNPVFRVGLREFIGKAKDSGVDGMLIVDLPVM---HASEFL 126
++ E + + VP ++M Y E + +A G +L DLP H ++
Sbjct: 71 SLAEEASQEAGVPYIVMAYATEQ-PWSFSEVLREASRKGALSVLPPDLPFELPGHVEWYV 129
Query: 127 EVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGTTGARDKIPETAFNLLKRA 186
E +R G++ A+P P L + +YL L TG K+P +K A
Sbjct: 130 EESRRLGMEPSLFASPKFPHRWLDRYRRLDPLLIYL-GLQPATGV--KLPLAFLRNVKTA 186
Query: 187 KRIC-KNKVAVGFGVSKREHVEMLLNAGANGVVVGSALINIIA 228
++I + + GF + E +L AGA+ VVVGS + +++
Sbjct: 187 RKIVGQVYMLAGFSIKSPEDALRVLEAGADAVVVGSEVARLVS 229
>gb|AAD04355.1| (AF084887) mutant tryptophan synthase [Neurospora crassa]
Length = 193
Score = 79.2 bits (192), Expect = 4e-14
Identities = 49/130 (37%), Positives = 71/130 (53%), Gaps = 2/130 (1%)
Query: 109 VDGMLIVDLPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGT 168
V+G +IVDLP A F ++ G+ V L AP T D R++ + + + F++++S G
Sbjct: 1 VNGFIIVDLPPEEAVSFRQLCTRGGLSYVPLIAPATSDARMRVLCQLADSFIHVVSRQGV 60
Query: 169 TGARDKIPETAFNLLKRAKRICKNK-VAVGFGVSKREHVEMLLNAGANGVVVGSALINII 227
TGA + LL R K+ NK AVGFGVS +H + A A+GVVVGS +I I
Sbjct: 61 TGASGTLNANLPELLARVKKYSGNKPAAVGFGVSTHDHFTQ-VGAIADGVVVGSMIITTI 119
Query: 228 AEHGENAEEK 237
+ + E K
Sbjct: 120 QKAAKGEEVK 129
>gb|AAD04356.1| (AF084888) mutant tryptophan synthase [Neurospora crassa]
Length = 193
Score = 78.8 bits (191), Expect = 6e-14
Identities = 49/130 (37%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
Query: 109 VDGMLIVDLPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGT 168
V+G +IVDLP A F ++ G+ V L AP T D R++ + + + F+Y++S G
Sbjct: 1 VNGFIIVDLPPEEAVSFRQLCTRGGLSYVPLIAPATSDARMRVLCQLADSFIYVVSRQGV 60
Query: 169 TGARDKIPETAFNLLKRAKRICKNK-VAVGFGVSKREHVEMLLNAGANGVVVGSALINII 227
GA + LL R K+ NK AVGFGVS +H + A A+GVVVGS +I I
Sbjct: 61 IGASGTLNANLPELLARVKKYSGNKPAAVGFGVSTHDHFTQ-VGAIADGVVVGSMIITTI 119
Query: 228 AEHGENAEEK 237
+ + E K
Sbjct: 120 QKAAKGEEVK 129
>gb|AAD04357.1| (AF084889) mutant tryptophan synthase [Neurospora crassa]
Length = 193
Score = 78.4 bits (190), Expect = 7e-14
Identities = 49/130 (37%), Positives = 70/130 (53%), Gaps = 2/130 (1%)
Query: 109 VDGMLIVDLPVMHASEFLEVAREEGIKTVFLAAPNTPDERLKEIDKASTGFVYLISLYGT 168
V+G +IVDLP A F ++ G+ V L AP T D R++ + + + F+Y++S G
Sbjct: 1 VNGFIIVDLPPEEAVSFRQLCTRGGLSYVPLIAPATSDARMRVLCQLADSFIYVVSRQGV 60
Query: 169 TGARDKIPETAFNLLKRAKRICKNK-VAVGFGVSKREHVEMLLNAGANGVVVGSALINII 227
TGA + LL R K+ NK AV FGVS +H + A A+GVVVGS +I I
Sbjct: 61 TGASGTLNANLPELLARVKKYSGNKPAAVDFGVSTHDHFTQ-VGAIADGVVVGSMIITTI 119
Query: 228 AEHGENAEEK 237
+ + E K
Sbjct: 120 QKAAKGEEVK 129
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.140 0.393
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88839084
Number of Sequences: 2977
Number of extensions: 3629514
Number of successful extensions: 9127
Number of sequences better than 1.0e-10: 108
Number of HSP's better than 0.0 without gapping: 82
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 8824
Number of HSP's gapped (non-prelim): 109
length of query: 248
length of database: 189,106,746
effective HSP length: 56
effective length of query: 192
effective length of database: 155,591,474
effective search space: 29873563008
effective search space used: 29873563008
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 164 (68.3 bits)