BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2092 (TRM1) DE:N2,N2-dimethylguanosine tRNA
methyltransferase, putative
(383 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75153 probable n2,n2-dimethylguanosine tRNA methyltransfer... 764 0.0
pir||E71194 probable N2,N2-dimethylguanosine tRNA methyltransfer... 652 0.0
pir||T51778 tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) [... 623 e-177
pir||B64418 N2,N2-dimethylguanosine tRNA methyltransferase (EC 2... 228 7e-59
pir||G69025 N2,N2-dimethylguanosine tRNA methyltransferase - Met... 225 8e-58
gb|AAK41219.1| N2,N2 dimethylguanosine tRNA methyltransferase [S... 219 4e-56
pir||G70372 N2,N2-dimethylguanosine tRNA methyltransferase - Aqu... 217 2e-55
gb|AAG37224.1|AF298223_2 (AF298223) N2,N2-dimethylguanosine tRNA... 215 7e-55
gi|11498421 N2,N2-dimethylguanosine tRNA methyltransferase (trm1... 201 1e-50
gb|AAG20235.1| (AE005099) N2,N2-dimethylguanosine tRNA methyltra... 191 1e-47
pir||H72669 probable N2,N2-dimethylguanosine tRNA methyltransfer... 186 4e-46
emb|CAC12126.1| (AL445066) N2, N2-dimethylguanosine tRNA methylt... 179 4e-44
gb|AAF53189.1| (AE003636) CG6388 gene product [Drosophila melano... 164 2e-39
pir||T27530 hypothetical protein ZC376.5 - Caenorhabditis elegan... 161 1e-38
pir||T46565 tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) [... 153 2e-36
emb|CAA20101.2| (AL031158) N2,N2-dimethylguanosine tRNA methyltr... 153 3e-36
pir||T39993 N2,N2-dimethylguanosine tRNA methyltransferase - fis... 153 3e-36
pir||T51410 N2,N2-dimethylguanine tRNA methyltransferase-like pr... 153 4e-36
gb|AAD29858.1| (AF086825) N2,N2-dimethylguonasine tRNA methyltra... 152 5e-36
gi|6320325 N2,N2-dimethylguanosine-specific tRNA methyltransfera... 152 5e-36
gb|AAD33093.1|AF081560_1 (AF081560) N2,N2-dimethylguanosine-spec... 150 3e-35
gb|AAD29859.1| (AF086826) N2,N2-dimethylguonasine tRNA methyltra... 150 3e-35
gb|AAF02109.1|AC009755_2 (AC009755) putative N2,N2-dimethylguano... 149 4e-35
gb|AAG12600.1|AC068900_6 (AC068900) putative N2,N2-dimethylguano... 149 4e-35
gb|AAD33092.1|AF081559_1 (AF081559) N2,N2-dimethylguanosine-spec... 148 7e-35
gb|AAC33150.1| (AC005546) R29425_1 [Homo sapiens] 137 2e-31
gi|8923219 hypothetical protein FLJ20244 [Homo sapiens] >gi|1142... 137 2e-31
pir||T47750 N2,N2-dimethylguanosine tRNA methyltransferases-like... 133 3e-30
sp|P20300|YORZ_PYRWO HYPOTHETICAL PROTEIN IN GAPDH 5'REGION (ORF... 120 2e-26
emb|CAC00605.2| (AL359776) N2,N2-dimethylguanosine tRNA methyltr... 107 3e-22
emb|CAC00613.1| (AL109865) bG120K12.3.1 (novel protein similar t... 93 6e-18
emb|CAC00614.1| (AL109865) bG120K12.3.2 (novel protein similar t... 93 6e-18
>pir||C75153 probable n2,n2-dimethylguanosine tRNA methyltransferas PAB2092 -
Pyrococcus abyssi (strain Orsay)
>gi|5457816|emb|CAB49306.1| (AJ248284)
N2,N2-dimethylguanosine tRNA methyltransferase, putative
[Pyrococcus abyssi]
Length = 383
Score = 764 bits (1951), Expect = 0.0
Identities = 383/383 (100%), Positives = 383/383 (100%)
Query: 1 MVITLELVEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVLKPRIVLDALS 60
MVITLELVEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVLKPRIVLDALS
Sbjct: 1 MVITLELVEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVLKPRIVLDALS 60
Query: 61 ATGIRGIRFALETPAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVV 120
ATGIRGIRFALETPAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVV
Sbjct: 61 ATGIRGIRFALETPAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVV 120
Query: 121 NHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPK 180
NHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPK
Sbjct: 121 NHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPK 180
Query: 181 ACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG 240
ACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG
Sbjct: 181 ACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG 240
Query: 241 ARKGDESLENLGYIYFDESTGKFEVERSFLPSKPNAYGPVWLGPLKSQEIVEEMLEISQQ 300
ARKGDESLENLGYIYFDESTGKFEVERSFLPSKPNAYGPVWLGPLKSQEIVEEMLEISQQ
Sbjct: 241 ARKGDESLENLGYIYFDESTGKFEVERSFLPSKPNAYGPVWLGPLKSQEIVEEMLEISQQ 300
Query: 301 LSLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTH 360
LSLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTH
Sbjct: 301 LSLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTH 360
Query: 361 FSPTGVKTDAPYEVFVNVLSSAK 383
FSPTGVKTDAPYEVFVNVLSSAK
Sbjct: 361 FSPTGVKTDAPYEVFVNVLSSAK 383
>pir||E71194 probable N2,N2-dimethylguanosine tRNA methyltransferase -
Pyrococcus horikoshii >gi|3258265|dbj|BAA30948.1|
(AP000007) 381aa long hypothetical
N2,N2-dimethylguanosine tRNA methyltransferase
[Pyrococcus horikoshii]
Length = 381
Score = 652 bits (1665), Expect = 0.0
Identities = 303/378 (80%), Positives = 349/378 (92%)
Query: 2 VITLELVEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVLKPRIVLDALSA 61
++ LEL+EV EGKAKIL PKAESIYD+PVFYNPRMALNRDI VVLLN+L P+IVLDALSA
Sbjct: 1 MVNLELIEVQEGKAKILIPKAESIYDSPVFYNPRMALNRDIVVVLLNILNPKIVLDALSA 60
Query: 62 TGIRGIRFALETPAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVN 121
TGIRGIRFALETPAEE+W+NDISEDAY LMK+NV+LNF GEL ES GRA+LK EKT+V+N
Sbjct: 61 TGIRGIRFALETPAEEVWLNDISEDAYELMKRNVMLNFDGELRESKGRAILKGEKTIVIN 120
Query: 122 HDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKA 181
HDDANRLMAE+HRYFHFIDLDPFGSPMEFLDTALRS KR+GILG+TATDGAPLCGAHP+A
Sbjct: 121 HDDANRLMAERHRYFHFIDLDPFGSPMEFLDTALRSAKRRGILGVTATDGAPLCGAHPRA 180
Query: 182 CMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGA 241
C+RKYLAVPLRGELCHEVGTRILVGV+ARYAAKYDLGI+VILAYYKDHYFRAF+KLKDGA
Sbjct: 181 CLRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGIDVILAYYKDHYFRAFVKLKDGA 240
Query: 242 RKGDESLENLGYIYFDESTGKFEVERSFLPSKPNAYGPVWLGPLKSQEIVEEMLEISQQL 301
RKGDE+LE LGYIYFD+ TGKFE+E+ FLP++PNAYGPVWLGPLK ++IV +M++ ++ L
Sbjct: 241 RKGDETLEKLGYIYFDDKTGKFELEQGFLPTRPNAYGPVWLGPLKDEKIVSKMVKEAESL 300
Query: 302 SLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHF 361
SLARKKQA+KLLK++ ELD+PLFYDTH +GRRLKIE +K+EEII+ LR GY A+RTHF
Sbjct: 301 SLARKKQALKLLKMIDQELDIPLFYDTHAIGRRLKIETKKVEEIISALREQGYEATRTHF 360
Query: 362 SPTGVKTDAPYEVFVNVL 379
SPTG+KT APYEVF+ +
Sbjct: 361 SPTGIKTSAPYEVFIETI 378
>pir||T51778 tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) [validated] -
Pyrococcus furiosus >gi|3420231|gb|AAC31882.1|
(AF051912) tRNA (guanine-26,N2-N2) methyltransferase
[Pyrococcus furiosus]
Length = 381
Score = 623 bits (1589), Expect = e-177
Identities = 293/378 (77%), Positives = 346/378 (91%), Gaps = 1/378 (0%)
Query: 3 ITLELVEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVLKPRIVLDALSAT 62
+++EL EV EGKAK+L PKA++IYD+PVFYNPRMA NRD+ V+LLNVLKP+IVLDALSAT
Sbjct: 1 MSMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSAT 60
Query: 63 GIRGIRFALETPAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNH 122
GIRGIRFALETPAEEIWMNDI+E AY LMKKNVLLNFKG L+E+ RA+ + EKT+V+N+
Sbjct: 61 GIRGIRFALETPAEEIWMNDINELAYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINN 120
Query: 123 DDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKAC 182
DDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILG+TATDGAPLCGAHPKAC
Sbjct: 121 DDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKAC 180
Query: 183 MRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGAR 242
+RKYLAVPLRGELCHEVGTRILVGV+ARYAAKYDLG+EV+LAYYKDHYFRAF+KLKDGA+
Sbjct: 181 LRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDHYFRAFVKLKDGAK 240
Query: 243 KGDESLENLGYIYFDESTGKFEVERSFLPSKPNAYGPVWLGPLKSQEIVEEMLE-ISQQL 301
KGDE+LENLGY+YFDE TG+FEVE+SFL ++PNAYGP+WLGPLK++++V EMLE +
Sbjct: 241 KGDETLENLGYVYFDEKTGRFEVEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGF 300
Query: 302 SLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHF 361
+A ++ +KLL +L +ELD+PLFYDTH LG+RLKIE +K+ EII EL+S+GY A+RTHF
Sbjct: 301 EVANYREVLKLLHMLHEELDIPLFYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHF 360
Query: 362 SPTGVKTDAPYEVFVNVL 379
SPTG+KT+APYEVFV V+
Sbjct: 361 SPTGIKTNAPYEVFVEVM 378
>pir||B64418 N2,N2-dimethylguanosine tRNA methyltransferase (EC 2.1.1.-) -
Methanococcus jannaschii >gi|1591613|gb|AAB98948.1|
(U67538) N2,N2-dimethylguanosine tRNA methyltransferase
(trm1) [Methanococcus jannaschii]
Length = 374
Score = 228 bits (576), Expect = 7e-59
Identities = 130/355 (36%), Positives = 210/355 (58%), Gaps = 34/355 (9%)
Query: 30 VFYNPRMALNRDIAVVL----LNVLKPR---IVLDALSATGIRGIRFALETPAE---EIW 79
VFYNPRM RDI++ + LN+ R + DAL+ +GIRG+R+A E +++
Sbjct: 24 VFYNPRMKTCRDISIAVIQAFLNLYHKRDKFYIADALAGSGIRGLRYAKELEFNGELKVF 83
Query: 80 MNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEKHRYFHFI 139
+NDI+ AY + N LN E+E + V ++DAN +++ R+F+ +
Sbjct: 84 LNDINPKAYEKIINNAKLN---EIENID------------VFNEDANTFLSKHFRFFNVV 128
Query: 140 DLDPFGSPMEFLDTALRS-VKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHE 198
D+DPFGSP +++ A+R+ V R G+L +TATD A LCG K+C+RKYLA PL G CHE
Sbjct: 129 DIDPFGSPAPYVEQAIRALVTRNGLLCLTATDTAALCGRSKKSCLRKYLAYPLFGRDCHE 188
Query: 199 VGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDESLENLGYIYFDE 258
R+LVG V R A KY+L ++ + + DHY R ++ GA++ D+ E LGY+ +
Sbjct: 189 FALRVLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEMLGYV---K 245
Query: 259 STGKFEVERSFLPSKPNAY-GPVWLGPLKSQEIVEEMLEISQQLSLARKKQAVKLLKILK 317
++ + F + GP+++G L + +VEE L+I+++ + ++ +K+L +K
Sbjct: 246 DVNGIKIIKKFEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKREFS--ERVLKILNAIK 303
Query: 318 DELDVPLF--YDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDA 370
E + YDTH +G+ LKI +++IIN+L+ +G+ A TH++P G+KTDA
Sbjct: 304 GESAINQVGCYDTHQIGKMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDA 358
>pir||G69025 N2,N2-dimethylguanosine tRNA methyltransferase - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622298|gb|AAB85679.1| (AE000887)
N2,N2-dimethylguanosine tRNA methyltransferase
[Methanobacterium thermoautotrophicum]
Length = 380
Score = 225 bits (567), Expect = 8e-58
Identities = 131/381 (34%), Positives = 198/381 (51%), Gaps = 33/381 (8%)
Query: 7 LVEVLEGKAKILTPKAESIYD-APVFYNPRMALNRDIAVVLLNVLKPRI-----VLDALS 60
++ V EG I P + APVFYNP M NRD++VV L V + + V D S
Sbjct: 1 MITVNEGSVTIRVPDFSKVSARAPVFYNPAMEFNRDVSVVALQVFQRLLGGEISVADTFS 60
Query: 61 ATGIRGIRFALETPA-EEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLV 119
+GIR IR+ +E E + NDI+ A +K N ++N +
Sbjct: 61 GSGIRAIRYLVEVEGVSEAFANDINPLAVECIKNNSVIN----------------SVSPE 104
Query: 120 VNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHP 179
V+ +DA+ + H F ID+DPFG+P F+D+A S + +L +TATD + LCG +
Sbjct: 105 VSREDASIFLRSNHGRFDVIDIDPFGTPAPFMDSAAASARNNSLLAVTATDTSSLCGTYI 164
Query: 180 KACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKD 239
K C+RKY + PL+ E CHE G RIL G A A+Y V+L++ HY R +I+++
Sbjct: 165 KPCLRKYSSRPLKTEYCHETGLRILAGFTAMNLARYRKAASVLLSHSSQHYMRLYIRVRR 224
Query: 240 GARKGDESLENLGYIYFDESTGKFEVERSFLPSK---------PNAYGPVWLGPLKSQEI 290
GAR+ DES+ N+G++ E F P K + GP+W+G ++ +
Sbjct: 225 GARRADESIRNIGFMLHCFRCLHHEHVNGFAPLKRECPHCGAEMDVAGPLWVGDIQDSKF 284
Query: 291 VEEMLEISQQLSLARKKQAVKLLKILKDELDVPL-FYDTHGLGRRLKIEARKIEEIINEL 349
+ EM+ + L +KLLK DE +P FYD H + +L A + ++++ L
Sbjct: 285 IGEMIGEVENKELNTAGDVLKLLKGCLDEAGMPPGFYDIHEVCSKLGRSAPPLRDVMDGL 344
Query: 350 RSLGYRASRTHFSPTGVKTDA 370
+ G+R SRTH PTG++TDA
Sbjct: 345 EAAGFRVSRTHIRPTGIRTDA 365
>gb|AAK41219.1| N2,N2 dimethylguanosine tRNA methyltransferase [Sulfolobus
solfataricus]
Length = 378
Score = 219 bits (552), Expect = 4e-56
Identities = 142/399 (35%), Positives = 231/399 (57%), Gaps = 41/399 (10%)
Query: 5 LELVEVLEGKAKILTPK-----AESIYD---APVFYNPRMALNRDIAVVLLNVLKPRIVL 56
++L EV EGK +I P E +D APVFYNP+M NRD++V+++++LKP+I++
Sbjct: 1 MKLKEVTEGKVRIFVPDPTGYMVEGKFDPSWAPVFYNPKMTFNRDLSVIVVSILKPKIIV 60
Query: 57 DALSATGIRGIRFALET-PAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSE 115
DALSATGIRGIR+ +E+ +E++ +ND + +A +L++ N ++NG K
Sbjct: 61 DALSATGIRGIRYYVESWKSEQLILNDKNPNATSLIQINA---------KNNGIENAK-- 109
Query: 116 KTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLC 175
+ + DAN L+ E +ID+DPFGSP F+ ++L + R GI+ TATD +PL
Sbjct: 110 ----IYNKDANALLYEVKS--DYIDIDPFGSPAPFILSSLNAATRNGIVAFTATDLSPLE 163
Query: 176 GAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFI 235
G+ P +C RKY A+ + E+G R+L+G + R AA + + + ++Y D+Y+R F+
Sbjct: 164 GSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREAAILEKTVYPLFSFYADYYYRLFV 223
Query: 236 KLKDGARKGDESLENLGYIYFDESTG---KFEVERSFLPSKP------NAYGPVWLGPLK 286
++++GARK D+++ N YF E + VE + P + GP+++GPL
Sbjct: 224 RVENGARKADDNI-NKHLKYFGECPRCGFQTFVEENCKTKCPVCGEIFSIIGPLYIGPLY 282
Query: 287 SQEIVEEMLEISQQLS-LARKKQAVKLLKILKDELDVP-LFYDTHGLGRRLKIEARKIEE 344
S E ++ ++++ + L + KLL +++ E +FY+ L +LKI A I
Sbjct: 283 SMEFLKRIMDLYSNFNYLTSFNRIQKLLNVIEKEARFKNVFYNISKLASKLKISA--IPP 340
Query: 345 IINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVLSSAK 383
I + L LG ASRTHF+PTG++TD YE + S +
Sbjct: 341 IESILECLG-DASRTHFAPTGIRTDKEYEEITKCIKSLR 378
>pir||G70372 N2,N2-dimethylguanosine tRNA methyltransferase - Aquifex aeolicus
>gi|2983391|gb|AAC06976.1| (AE000710)
N2,N2-dimethylguanosine tRNA methyltransferase [Aquifex
aeolicus]
Length = 392
Score = 217 bits (546), Expect = 2e-55
Identities = 144/394 (36%), Positives = 210/394 (52%), Gaps = 38/394 (9%)
Query: 10 VLEGKAKILTPKAESIY--DAPVFYNPRMALNRDIAVVLLNVL-----KPRIVLDALSAT 62
V EG AKI+ P+ D PVFYNPRM +NRD+AV+ L L +P V D LSA+
Sbjct: 4 VQEGIAKIIVPEIPKTVSSDMPVFYNPRMRVNRDLAVLGLEYLCKKLGRPVKVADPLSAS 63
Query: 63 GIRGIRFALETPA-EEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVN 121
GIR IRF LET E+ + NDIS A +MK+N LN E ++
Sbjct: 64 GIRAIRFLLETSCVEKAYANDISSKAIEIMKENFKLNNI-------------PEDRYEIH 110
Query: 122 HDDANRLMAEKHRY-FHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPK 180
+AN + ++ + F ++DLDPFG+P+ F+++ S+KR GIL +TATD APL G +PK
Sbjct: 111 GMEANFFLRKEWGFGFDYVDLDPFGTPVPFIESVALSMKRGGILSLTATDTAPLSGTYPK 170
Query: 181 ACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG 240
CMR+Y+A PLR E HEVG RIL+ V AA+YD+ + I AY HYF+ F + G
Sbjct: 171 TCMRRYMARPLRNEFKHEVGIRILIKKVIELAAQYDIAMIPIFAYSHLHYFKLFFVKERG 230
Query: 241 ARKGDESLENLGYIYF---------DESTGKFEVERSFLPSKPNAYGPVWLGPLKSQEIV 291
K D+ +E GYI + KF+ + SK + GP+W+G L +E
Sbjct: 231 VEKVDKLIEQFGYIQYCFNCMNREVVTDLYKFKEKCPHCGSKFHIGGPLWIGKLWDEEFT 290
Query: 292 EEMLEISQQLSLARKKQAVKLLKILKDE--LDVPLFYDTHGLGRRLKIEARKIEEIINEL 349
+ E +Q+ +K+ ++LK++K+E L FY L ++K+ A+ I +
Sbjct: 291 NFLYEEAQKRE-EIEKETKRILKLIKEESQLQTVGFYVLSKLAEKVKLPAQPPIRIAVKF 349
Query: 350 RSLGYRASRTHFSPTGVKTDAPYEVFVNVLSSAK 383
+ RTHF G +T+ +E + + K
Sbjct: 350 ----FNGVRTHFVGDGFRTNLSFEEVMKKMEELK 379
>gb|AAG37224.1|AF298223_2 (AF298223) N2,N2-dimethylguanosine tRNA methyltransferase
[Methanococcus maripaludis]
Length = 373
Score = 215 bits (542), Expect = 7e-55
Identities = 131/385 (34%), Positives = 219/385 (56%), Gaps = 36/385 (9%)
Query: 10 VLEGKAKILTPKAESIYDAP-VFYNPRMALNRDIAVVLLNVLKPR------IVLDALSAT 62
+ EG+ K++ P+ ++ VFYNP M NRDI+V ++ ++ D L +
Sbjct: 4 ISEGETKLMVPEESTLSKKDTVFYNPVMETNRDISVSVVQSFLDDFKRDEFLMCDPLGGS 63
Query: 63 GIRGIRFALETPAE---EIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLV 119
G RGIR+A E ++ + DI+ A ++K+N+ LN ELE +
Sbjct: 64 GARGIRYAKELKFNGDLKVSIGDINPSAVKMIKENLKLN---ELEN------------VE 108
Query: 120 VNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALR-SVKRKGILGITATDGAPLCGAH 178
V H+DAN L+++ + F+ +DLDPFGSP+ +LD+ +R S+ + G+L +TATD A LCGA+
Sbjct: 109 VFHEDANVLLSKNFKVFNVVDLDPFGSPVPYLDSGIRASLTKGGLLCMTATDTAVLCGAY 168
Query: 179 PKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLK 238
K C+RKY AVPL+G+ E+ R+++G + A+KYD+G++ I ++ DHY R F+ +
Sbjct: 169 RKTCIRKYNAVPLKGD--KELAVRLMIGYAVKMASKYDIGLKPIFSHVTDHYARTFMVTE 226
Query: 239 DGARKGDESLENLGYIYFDESTGKFEVERSFLPSKPNAY-GPVWLGPLKSQEIVEEMLEI 297
GA K D ++ENLGYI D F ++F Y GP +LG + + IV+ L
Sbjct: 227 RGAGKADSAIENLGYIRQDSEQKSF---KTFEEGSEKGYAGPFYLGEISDKNIVQNALNT 283
Query: 298 SQQLSLARKKQAVKLLKILKDELDVPLF--YDTHGLGRRLKIEARKIEEIINELRSLGYR 355
++ + + K+AV +L+++ E ++ +D H L +K + +I+ L+ G++
Sbjct: 284 AKTRNYS--KRAVNILEMISRESEINQVGCFDIHELCSFIKKLVPPVNDIMENLKENGFK 341
Query: 356 ASRTHFSPTGVKTDAPYEVFVNVLS 380
+R H++P G+KTDA V ++S
Sbjct: 342 VTRVHYNPYGLKTDAELSDLVVLIS 366
>gi|11498421 N2,N2-dimethylguanosine tRNA methyltransferase (trm1)
[Archaeoglobus fulgidus] >gi|7450759|pir||G69351
N2,N2-dimethylguanosine tRNA methyltransferase (trm1)
homolog - Archaeoglobus fulgidus
>gi|2649794|gb|AAB90427.1| (AE001048)
N2,N2-dimethylguanosine tRNA methyltransferase (trm1)
[Archaeoglobus fulgidus]
Length = 349
Score = 201 bits (506), Expect = 1e-50
Identities = 132/384 (34%), Positives = 203/384 (52%), Gaps = 45/384 (11%)
Query: 8 VEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVLKPRIVLDALSATGIRGI 67
+EV EG+A++ K E VFYNPRM RD+ +++ + + DALSA+GIRGI
Sbjct: 1 MEVEEGRARV---KVEG-----VFYNPRMRFCRDLDMLVFATMDSKEYFDALSASGIRGI 52
Query: 68 RFALETPAEEIWMNDISEDAYNLMKKNVLLN-FKGELEESNGRAVLKSEKTLVVNHDDAN 126
R ALE + ++ ND+S A ++++N+ N GE+ + AV++
Sbjct: 53 RAALEAGKKAVF-NDVSPKAVKVIEENLRENGVSGEVINGDAAAVMRQ------------ 99
Query: 127 RLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKY 186
R F ID+DPFGSP F+D+A S KR L +TATD A LCG+ + ++KY
Sbjct: 100 -------RAFEHIDIDPFGSPAPFMDSACFSAKR--YLSVTATDTAALCGSATNSGLKKY 150
Query: 187 LAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDE 246
A ++ ++ HEVG R+L+G V R A KY+ + ++++ ++HY+R K+K +
Sbjct: 151 GAFAVKTDVYHEVGLRMLIGFVVREATKYEKALFPLISWVREHYYRVHFKIKKSTAMSAK 210
Query: 247 SLENLGYIYFDESTGKFEV-------ERSFLPSKPNAYGPVWLGPLKSQEIVEEMLEISQ 299
E +GY+ + + + +V ER K + GP+WLG LK ++ E++ E
Sbjct: 211 VYEKMGYLAYCSTCLRKKVLGMGEGAERCECGGKFSLIGPIWLGELKQRDFAEKVAE--- 267
Query: 300 QLSLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRT 359
A K L KIL E+D P Y L + + + ++ EL+ LGY ASRT
Sbjct: 268 ---KAEGKLRAFLEKILA-EIDAPTAYSLPALAKIHSLTLPPTDVVVEELKKLGYEASRT 323
Query: 360 HFSPTGVKTDAPYEVFVNVLSSAK 383
H+ VKTDA +V V +L S K
Sbjct: 324 HYCGFCVKTDADRDVVVKILRSRK 347
>gb|AAG20235.1| (AE005099) N2,N2-dimethylguanosine tRNA methyltransferase; Trm1
[Halobacterium sp. NRC-1]
Length = 371
Score = 191 bits (480), Expect = 1e-47
Identities = 128/381 (33%), Positives = 188/381 (48%), Gaps = 40/381 (10%)
Query: 8 VEVLEGKAKILTPKAESIYDAP---VFYNPRMALNRDIAVVLLNVLKPR-----IVLDAL 59
++V EG + P A + VF+N LNRD+ V L + R LDA+
Sbjct: 1 MDVSEGGVTVSVPTASGGGEGAADDVFFNATQELNRDLTVATLAAFREREPRAASYLDAM 60
Query: 60 SATGIRGIRFALETPAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLV 119
+A+GIRG+R A ++ M D+ DA L N+ N +G V +
Sbjct: 61 TASGIRGVRAA--NAGWDVTMADVDADAVELATSNLARN------GLDGEVVAR------ 106
Query: 120 VNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHP 179
DAN L+ + R +D+DPFGSPM F D A + + ++ +TATD APLCGAH
Sbjct: 107 ----DANSLLHDHDRVLDVVDIDPFGSPMPFADAAFANARN--LVCVTATDTAPLCGAHF 160
Query: 180 KACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKD 239
++ +RKY A P E+G RIL+G +AR AA+YD+G+ IL++ DHY R +++L
Sbjct: 161 ESGVRKYSATPRNTNYHAEMGLRILLGALARTAARYDVGVTPILSHVSDHYVRTYLELDH 220
Query: 240 GARKGDESLENLGYIYF-------DESTGKFEVERSFLP----SKPNAYGPVWLGPLKSQ 288
A + ++E +G++Y D G R+ P + GP+WLGP +
Sbjct: 221 RATDANAAVEQVGHVYHCQQCLSRDHEYGLVAAPRAECPRCGGHQVLTAGPLWLGPAHEE 280
Query: 289 EIVEEMLEISQQLSLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINE 348
+ E + QA LL+ ++ EL P YD H L + A + E +++
Sbjct: 281 AFAASVRE-QLTREMGEASQAFHLLQTIEGELHEPTHYDQHRLYSQWGEPAVGMAEFLDQ 339
Query: 349 LRSLGYRASRTHFSPTGVKTD 369
LR G RASRTHF T KTD
Sbjct: 340 LRDAGLRASRTHFGGTTFKTD 360
>pir||H72669 probable N2,N2-dimethylguanosine tRNA methyltransferase APE0782 -
Aeropyrum pernix (strain K1) >gi|5104445|dbj|BAA79760.1|
(AP000060) 409aa long hypothetical
N2,N2-dimethylguanosine tRNA methyltransferase
[Aeropyrum pernix]
Length = 409
Score = 186 bits (467), Expect = 4e-46
Identities = 120/364 (32%), Positives = 189/364 (50%), Gaps = 31/364 (8%)
Query: 30 VFYNPRMALNRDIAVVLLNVLKPR-------IVLDALSATGIRGIRFALETPAEEIWMND 82
VFYNP M NRD++VV + L+ + DA + G+RG+R+A+E ++ MND
Sbjct: 42 VFYNPAMEFNRDVSVVAASALRRTGLLTRSGVAFDAHAGVGVRGVRYAVEAGYVKVIMND 101
Query: 83 ISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEKHRYFHFIDLD 142
I+ A L LN + E + E ++ H L E+ ID+D
Sbjct: 102 INPKASML----AALNARANGLEPGSYMIFNKESNSLMFH-----LSRERPTPVSLIDID 152
Query: 143 PFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPL-RGELCHEVGT 201
P+GSP F+D AL + ++ +TATD A L G +A +R+Y+ + + + E G
Sbjct: 153 PYGSPAPFVDAALALSGKGTVVAMTATDLAVLEGGKARAAVRRYMLRSVSKTPVSKETGL 212
Query: 202 RILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDESL-ENLGYIYFDEST 260
R+L+G VAR AA +D ++ +LAYY DHY+R ++ ++ GAR+ D L ENLG + + T
Sbjct: 213 RVLLGYVARVAAAHDKAVKPLLAYYADHYYRVYVAVERGARRSDSMLEENLGRLVYCPET 272
Query: 261 GKFEVERSFLPSKPNAYG--------PVWLGPLKSQEIVEEMLEIS-QQLSLARKKQAVK 311
G + S+ +A G P W+G L Q +E ML I+ + + L + + K
Sbjct: 273 G-VALALSYAEDPASACGGSYVVAADPAWIGSLGDQAFLEAMLNIAVEAVWLGTRPRVEK 331
Query: 312 LLKILKDELDV---PLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKT 368
LL L E + ++ + + ++ K +++ LRS+GYRA THFS GV+T
Sbjct: 332 LLNTLHGEAPLSPRSIYVSLTSVASKARVNTPKKSKVVELLRSMGYRAVATHFSGEGVRT 391
Query: 369 DAPY 372
DAP+
Sbjct: 392 DAPW 395
>emb|CAC12126.1| (AL445066) N2, N2-dimethylguanosine tRNA methyltransferase related
protein [Thermoplasma acidophilum]
Length = 344
Score = 179 bits (450), Expect = 4e-44
Identities = 120/375 (32%), Positives = 186/375 (49%), Gaps = 48/375 (12%)
Query: 10 VLEGKAKILTPKAESIYDAPV-----FYNPRMALNRDIAVVLLNVLKPRIVLDALSATGI 64
V EG A+IL P+ +Y P FYN +NRDI + + + R VLD TGI
Sbjct: 3 VREGSAEILVPE---VYHGPGKRGTGFYNADQKINRDITIEFIKKMGIRNVLDGFGGTGI 59
Query: 65 RGIRFALETPAEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDD 124
RGIR + ET + + ++++S D+Y L++ NV E NG + V+N
Sbjct: 60 RGIRISKETDSA-VTISEVSPDSYRLIRDNV---------ERNG------SQASVINDSF 103
Query: 125 ANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMR 184
L +H + ++D+DP+GSP+ +LD AL VKR G LG+TATD + L G+ P
Sbjct: 104 ECVL---QHGAYEYVDVDPYGSPVPYLDAALMGVKRNGFLGVTATDQSALTGSVPHKTRI 160
Query: 185 KYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGARKG 244
+Y A+ HE+G R+L+G +A+ AA I+ +++ + HY+R F++++ G
Sbjct: 161 RYDALIKNDTFRHEMGIRLLIGYMAKRAASLGRFIDPLISIWHGHYYRVFVRVRKGFEGA 220
Query: 245 DESLENLGYIYFDESTGKFEVERSFLPSKPNAYGPVWLGPLKSQEIVEEMLEISQQLSLA 304
++NLGY+ K + GPVW G L+ + E +L +
Sbjct: 221 GRMMQNLGYV------NKHNLLSGIYQDMDE--GPVWKGNLQDNAVAEAVLASAGH---- 268
Query: 305 RKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPT 364
K E + LF D + R IE +I+ L S G+ ASRT FSPT
Sbjct: 269 ---------KAFNPEENRLLFCDLTDIARARHTSLPDIESVIDALSSSGHAASRTMFSPT 319
Query: 365 GVKTDAPYEVFVNVL 379
G+KTDA ++ +++
Sbjct: 320 GIKTDASCDLVESLM 334
>gb|AAF53189.1| (AE003636) CG6388 gene product [Drosophila melanogaster]
Length = 578
Score = 164 bits (410), Expect = 2e-39
Identities = 126/443 (28%), Positives = 202/443 (45%), Gaps = 97/443 (21%)
Query: 22 AESIYDAPVFYNPRMALNRDIAVVLLNVLKPRI--------------------------- 54
AE + VFYNP NRD+++ LNV + R+
Sbjct: 25 AEIVSGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALKKQRKKVKEQEDEKTTPV 84
Query: 55 ------------------VLDALSATGIRGIRFALETPA-EEIWMNDISEDAYNLMKKNV 95
+L+AL+ATG+R IR+A E +I ND+S A + N+
Sbjct: 85 PEDPPVYEAGTRYEDGLRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNI 144
Query: 96 LLNFKGELEESNGRAVLKSEKTLVVNHDDANRLM---AEKHRYFHFIDLDPFGSPMEFLD 152
N K E+ + +H DA LM + + F +DLDP+G P FLD
Sbjct: 145 RHN--------------KVEELIEPSHSDAMTLMYLSTQPEKRFDAVDLDPYGCPNRFLD 190
Query: 153 TALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYA 212
A++ + G+L +TATD A L G P+AC KY +VPLR + CHE+ RIL+ + +A
Sbjct: 191 GAMQCLVDGGLLLVTATDMAVLAGNAPEACYVKYGSVPLRMKCCHEMALRILLHCIESHA 250
Query: 213 AKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDESLENLGYIYF---------------- 256
+Y IE +L+ D Y R F+++ G + S+ +IY
Sbjct: 251 NRYGKYIEPLLSISADFYIRIFVRVYVGQAQCKLSMSKQSWIYQCTGCETFTLQPLGITK 310
Query: 257 -DESTGKFEVERSFLPSKP-------------NAYGPVWLGPLKSQEIVEEMLEISQQL- 301
+ + G + + +P+ P + GP+W P+ + E V+++L Q+
Sbjct: 311 PNPTAGNPQQLKFGIPTGPAVNSQCEHCGHRHHLGGPIWSAPIHNPEFVQDLLTAVQETT 370
Query: 302 --SLARKKQAVKLLKILKDEL-DVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASR 358
SL +++ V +L ++++EL DVPL+Y L LK+E + + + + GYR S
Sbjct: 371 LQSLGTQRRIVGVLSMVQEELQDVPLYYTPDKLCCVLKLEIVPMLKFRSAILHAGYRVSY 430
Query: 359 THFSPTGVKTDAPYEVFVNVLSS 381
+H S +KT+AP V ++L S
Sbjct: 431 SHASKNSLKTNAPPAVLWDILRS 453
>pir||T27530 hypothetical protein ZC376.5 - Caenorhabditis elegans
>gi|11359792|pir||T43053 N2,N2-dimethylguanosine tRNA
methyltransferase (EC 2.1.1.-) - Caenorhabditis elegans
>gi|3881410|emb|CAB00881.1| (Z77136) contains similarity
to Pfam domain: PF02005 (N2, N2-dimethylguanosine tRNA
methyltransferase), Score=935.5, E-value=4.8e-278,
N=1~cDNA EST EMBL:T02427 comes from this gene~cDNA EST
EMBL:T00178 comes from this gene~cDNA EST yk646c5.3
comes from> >gi|4249621|gb|AAD13737.1| (AF059608)
N2,N2-dimethylguanosine tRNA methyltransferase
[Caenorhabditis elegans]
Length = 526
Score = 161 bits (403), Expect = 1e-38
Identities = 123/442 (27%), Positives = 208/442 (46%), Gaps = 90/442 (20%)
Query: 6 ELVEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVL--------------- 50
E+ + EG+AK+ + PVFYNP NRD+ V +L
Sbjct: 20 EVTVIQEGQAKVG-------FHGPVFYNPVQEFNRDLTVTVLRQFSADHQKWAEEQKQLK 72
Query: 51 --------KPRI---------VLDALSATGIRGIRFALETP-AEEIWMNDISEDAYNLMK 92
K ++ +LDALSA+G+R +RF+ E P I ND S++A ++
Sbjct: 73 TEEEPPKKKNKLAINEDGKIRILDALSASGLRALRFSKEVPNVGFIMANDFSDNAVASIQ 132
Query: 93 KNVLLNFKGELEESN-GRAVLKSEKTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFL 151
+NV LN ++ E++ G AV+ ++ H ++ FH +DLDP+G+ FL
Sbjct: 133 ENVKLNGVEDIVEAHFGDAVMT-----MMEHRGIDKR-------FHAVDLDPYGTASTFL 180
Query: 152 DTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARY 211
D+A++ V +GIL +T TD A LCG P+AC KY AV R + CHEVG RIL+ +
Sbjct: 181 DSAVQCVADRGILMVTCTDMAVLCGNTPEACYNKYDAVTTRMKCCHEVGLRILLRAIDSA 240
Query: 212 AAKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDESLENLGYIYF--------------- 256
A +Y IE +++ D Y R F+++ GA + +S +G +
Sbjct: 241 ANRYTRYIEPLVSISVDFYVRVFVRVHTGAFQAKQSGTKVGTVLVCSGCHSMEPLVLLKR 300
Query: 257 ----DESTGKFEVERSFLPSKPN----------AYGPVWLGPLKSQEIVEEMLE------ 296
+S R + N GP++L P+ S+ V +LE
Sbjct: 301 GEGNQQSKYSIPTVRHSISGPGNRCIHCLLPLHQIGPIYLAPIHSKPFVTSLLERLKSTP 360
Query: 297 ISQQLSLARKKQAVKLLKILKDELDVPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRA 356
+++L + Q V L ++ +ELD L+Y+ + + +K+ K + + + + + G++
Sbjct: 361 EAERLGTHGRLQGV--LTMVNEELDDVLYYEHNQMANVVKVSVPKSQSVRSAILNAGFKV 418
Query: 357 SRTHFSPTGVKTDAPYEVFVNV 378
S +H +P +KT+AP + ++
Sbjct: 419 SGSHCNPRAIKTNAPMHLLWDI 440
>pir||T46565 tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) [imported] -
fission yeast (Schizosaccharomyces pombe)
>gi|3393025|emb|CAA11801.1| (AJ224000) tRNA
(Guanine-N2-)-Methyltransferase [Schizosaccharomyces
pombe]
Length = 548
Score = 153 bits (384), Expect = 2e-36
Identities = 116/360 (32%), Positives = 174/360 (48%), Gaps = 47/360 (13%)
Query: 55 VLDALSATGIRGIRFALETP-AEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLK 113
+L+ALSATG+R IR+A E P + I ND+ E+A ++KNV N ++
Sbjct: 127 ILEALSATGLRSIRYAKELPNVKRILANDLLENAVKTIEKNVNYNNVSDI---------- 176
Query: 114 SEKTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAP 173
++ N DAN +M ++ IDLDP+GS FLD A++SV + G+L IT TD A
Sbjct: 177 ----VIPNKGDANAVMHMNKFHYDVIDLDPYGSAAPFLDAAVQSVSKDGLLCITCTDSAV 232
Query: 174 LCG-AHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFR 232
L G A+P+ C Y LR CHE R L+ +A AAKY I+ +L+ D YFR
Sbjct: 233 LAGNAYPEKCFSNYGGSSLRSNFCHEQAVRHLLYAIAASAAKYGRAIKPLLSLSIDFYFR 292
Query: 233 AF--IKLKDGARKGDESLENLGY------IYFDESTGKFEVER----------SFLPSKP 274
F IK K K +S L Y + ++ GK R S P
Sbjct: 293 VFVQIKAKPVLVKNLQSQSLLIYHCSGCGTFAEQPLGKTSPGRLPGTTKFSNASGPPVSA 352
Query: 275 NAY---------GPVWLGPLKSQEIVEEMLEISQQLS---LARKKQAVKLLKILKDEL-D 321
N GP+W GPL E +++M I++ L K++ + +L + +EL D
Sbjct: 353 NCEHCGYVHHVGGPLWGGPLHDAEFLKKMRAIAEDLDPEVYGTKRRILGMLALADEELPD 412
Query: 322 VPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVLSS 381
VP ++ + L+ ++ ++ L + GYR S +H +KT+AP+ +VL S
Sbjct: 413 VPFYFVLSQICSVLRSQSPPQNIFVSALLNAGYRVSGSHAKSNAIKTNAPWSFVWDVLRS 472
>emb|CAA20101.2| (AL031158) N2,N2-dimethylguanosine tRNA methyltransferase
[Schizosaccharomyces pombe]
Length = 548
Score = 153 bits (383), Expect = 3e-36
Identities = 116/360 (32%), Positives = 174/360 (48%), Gaps = 47/360 (13%)
Query: 55 VLDALSATGIRGIRFALETP-AEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLK 113
+L+ALSATG+R IR+A E P + I ND+ E+A ++KNV N ++
Sbjct: 127 ILEALSATGLRSIRYAKELPNVKRILANDLLENAVKTIEKNVNYNNVSDI---------- 176
Query: 114 SEKTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAP 173
++ N DAN +M ++ IDLDP+GS FLD A++SV + G+L IT TD A
Sbjct: 177 ----VIPNKGDANAVMHMNKFHYDVIDLDPYGSAAPFLDAAVQSVSKDGLLCITCTDSAV 232
Query: 174 LCG-AHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFR 232
L G A+P+ C Y LR CHE R L+ +A AAKY I+ +L+ D YFR
Sbjct: 233 LAGNAYPEKCFSNYGGSSLRSNFCHEQAVRHLLYAIAASAAKYGRAIKPLLSLSIDFYFR 292
Query: 233 AF--IKLKDGARKGDESLENLGY------IYFDESTGKFEVER----------SFLPSKP 274
F IK K K +S L Y + ++ GK R S P
Sbjct: 293 VFVQIKAKPVLVKNLQSQSLLIYHCSGCGSFAEQPLGKTSPGRLPGTTKFSNASGPPVSA 352
Query: 275 NAY---------GPVWLGPLKSQEIVEEMLEISQQLS---LARKKQAVKLLKILKDEL-D 321
N GP+W GPL E +++M I++ L K++ + +L + +EL D
Sbjct: 353 NCEHCGYVHHVGGPLWGGPLHDAEFLKKMRAIAEDLDPEVYGTKRRILGMLALADEELPD 412
Query: 322 VPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVLSS 381
VP ++ + L+ ++ ++ L + GYR S +H +KT+AP+ +VL S
Sbjct: 413 VPFYFVLSQICSVLRSQSPPQNIFVSALLNAGYRVSGSHAKSNAIKTNAPWSFVWDVLRS 472
>pir||T39993 N2,N2-dimethylguanosine tRNA methyltransferase - fission yeast
(Schizosaccharomyces pombe)
Length = 524
Score = 153 bits (383), Expect = 3e-36
Identities = 116/360 (32%), Positives = 174/360 (48%), Gaps = 47/360 (13%)
Query: 55 VLDALSATGIRGIRFALETP-AEEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLK 113
+L+ALSATG+R IR+A E P + I ND+ E+A ++KNV N ++
Sbjct: 103 ILEALSATGLRSIRYAKELPNVKRILANDLLENAVKTIEKNVNYNNVSDI---------- 152
Query: 114 SEKTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAP 173
++ N DAN +M ++ IDLDP+GS FLD A++SV + G+L IT TD A
Sbjct: 153 ----VIPNKGDANAVMHMNKFHYDVIDLDPYGSAAPFLDAAVQSVSKDGLLCITCTDSAV 208
Query: 174 LCG-AHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFR 232
L G A+P+ C Y LR CHE R L+ +A AAKY I+ +L+ D YFR
Sbjct: 209 LAGNAYPEKCFSNYGGSSLRSNFCHEQAVRHLLYAIAASAAKYGRAIKPLLSLSIDFYFR 268
Query: 233 AF--IKLKDGARKGDESLENLGY------IYFDESTGKFEVER----------SFLPSKP 274
F IK K K +S L Y + ++ GK R S P
Sbjct: 269 VFVQIKAKPVLVKNLQSQSLLIYHCSGCGSFAEQPLGKTSPGRLPGTTKFSNASGPPVSA 328
Query: 275 NAY---------GPVWLGPLKSQEIVEEMLEISQQLS---LARKKQAVKLLKILKDEL-D 321
N GP+W GPL E +++M I++ L K++ + +L + +EL D
Sbjct: 329 NCEHCGYVHHVGGPLWGGPLHDAEFLKKMRAIAEDLDPEVYGTKRRILGMLALADEELPD 388
Query: 322 VPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVLSS 381
VP ++ + L+ ++ ++ L + GYR S +H +KT+AP+ +VL S
Sbjct: 389 VPFYFVLSQICSVLRSQSPPQNIFVSALLNAGYRVSGSHAKSNAIKTNAPWSFVWDVLRS 448
>pir||T51410 N2,N2-dimethylguanine tRNA methyltransferase-like protein -
Arabidopsis thaliana >gi|9755626|emb|CAC01780.1|
(AL391144) N2, N2-dimethylguanine tRNA
methyltransferase-like protein [Arabidopsis thaliana]
Length = 684
Score = 153 bits (382), Expect = 4e-36
Identities = 105/356 (29%), Positives = 169/356 (46%), Gaps = 42/356 (11%)
Query: 50 LKPRIVLDALSATGIRGIRFALETPA-EEIWMNDISEDAYNLMKKNVLLNFKGELEESNG 108
LKP VL+ALSA+G+R +R+A E ++ D + ++N+ F G + S
Sbjct: 217 LKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNI--KFNGLMSTSKV 274
Query: 109 RAVLKSEKTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITA 168
+ L + +++H + F +DLDP+G+P FLD+A++SV G+L TA
Sbjct: 275 ESHLTDARVHMLSHP----------KDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTA 324
Query: 169 TDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKD 228
TD A LCGA+ + C KY + PL+G+ CHE+ RIL+ + +A +Y I +L+ D
Sbjct: 325 TDMAVLCGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMD 384
Query: 229 HYFRAFIKLKDGARKGDESLENLGYIYFDESTGKFEVER-----------SFLP------ 271
Y R F+++ A + L Y+Y F ++ +LP
Sbjct: 385 FYVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVV 444
Query: 272 --------SKPNAYGPVWLGPLKSQEIVEEMLEISQQLSLARKKQAVKLLKILKDELDVP 323
K N GP+W P+ QE V +L + + K + +I + DVP
Sbjct: 445 PQDCTHCGKKYNMGGPIWSAPIHDQEWVNSILNGVKSM----KDRYPAYDRICAELPDVP 500
Query: 324 LFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
LF H L LK + + + + YR S +H +P G+KTDAP E+ +++
Sbjct: 501 LFLSLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIM 556
>gb|AAD29858.1| (AF086825) N2,N2-dimethylguonasine tRNA methyltransferase
[Saccharomyces cerevisiae]
Length = 554
Score = 152 bits (381), Expect = 5e-36
Identities = 130/464 (28%), Positives = 209/464 (45%), Gaps = 113/464 (24%)
Query: 10 VLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLL---------------------- 47
V EGKA+IL PK E+ VFYNP NRD++V +
Sbjct: 29 VKEGKAEILFPKKET-----VFYNPIQQFNRDLSVTCIKAWDNLYGEECGQKRNNKKSKK 83
Query: 48 ----------------------------NVLKPRI-VLDALSATGIRGIRFALETP-AEE 77
N +P I +L+ALSATG+R IR+A E P E
Sbjct: 84 KRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVRE 143
Query: 78 IWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEK---HR 134
+ ND+ +A +K+NV N E + N DDAN LM +
Sbjct: 144 VIANDLLPEAVESIKRNVEYN--------------SVENIVKPNLDDANVLMYRNKASNN 189
Query: 135 YFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCG-AHPKACMRKY-LAVPLR 192
FH IDLDP+G+ F+D A++S++ G++ +T TD + L G +P+ C Y A +
Sbjct: 190 KFHVIDLDPYGTVTPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVS 249
Query: 193 GELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG------------ 240
E HE R+++ ++ + AAKY +E +L+ D Y R F+K+K
Sbjct: 250 HESTHESALRLVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTM 309
Query: 241 -----ARKGDESLENLGYIYFDESTG-----KFEVER--------SFLPSKPNAYGPVWL 282
+R G + LG I E K+ V + F + GP++
Sbjct: 310 TTYHCSRCGSYHNQPLGRISQREGRNNKTFTKYSVAQGPPVDTKCKFCEGTYHLAGPMYA 369
Query: 283 GPLKSQEIVEEMLEIS------QQLSLARKKQAVKLLKILKDEL-DVPLFYDTHGLGRRL 335
GPL ++E +EE+L I+ Q + +K+ +L + K+EL D P ++ + + +
Sbjct: 370 GPLHNKEFIEEVLRINKEEHRDQDDTYGTRKRIEGMLSLAKNELSDSPFYFSPNHIASVI 429
Query: 336 KIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
K++ +++++ L SLG+ S TH P+ +KT+AP++ V+
Sbjct: 430 KLQVPPLKKVVAGLGSLGFECSLTHAQPSSLKTNAPWDAIWYVM 473
>gi|6320325 N2,N2-dimethylguanosine-specific tRNA methyltransferase; Trm1p
[Saccharomyces cerevisiae]
>gi|136242|sp|P15565|TRM1_YEAST N2,N2-DIMETHYLGUANOSINE
TRNA METHYLTRANSFERASE PRECURSOR >gi|83343|pir||A28323
N2,N2-dimethylguanine tRNA methyltransferase (EC
2.1.1.-) [validated] - yeast (Saccharomyces cerevisiae)
>gi|172940|gb|AAA35150.1| (M17193) tRNA
dimethyltransferase [Saccharomyces cerevisiae]
>gi|747894|emb|CAA88673.1| (Z48758) Trm1p [Saccharomyces
cerevisiae]
Length = 570
Score = 152 bits (381), Expect = 5e-36
Identities = 130/464 (28%), Positives = 209/464 (45%), Gaps = 113/464 (24%)
Query: 10 VLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLL---------------------- 47
V EGKA+IL PK E+ VFYNP NRD++V +
Sbjct: 45 VKEGKAEILFPKKET-----VFYNPIQQFNRDLSVTCIKAWDNLYGEECGQKRNNKKSKK 99
Query: 48 ----------------------------NVLKPRI-VLDALSATGIRGIRFALETP-AEE 77
N +P I +L+ALSATG+R IR+A E P E
Sbjct: 100 KRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVRE 159
Query: 78 IWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEK---HR 134
+ ND+ +A +K+NV N E + N DDAN LM +
Sbjct: 160 VIANDLLPEAVESIKRNVEYN--------------SVENIVKPNLDDANVLMYRNKATNN 205
Query: 135 YFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCG-AHPKACMRKY-LAVPLR 192
FH IDLDP+G+ F+D A++S++ G++ +T TD + L G +P+ C Y A +
Sbjct: 206 KFHVIDLDPYGTVTPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVS 265
Query: 193 GELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG------------ 240
E HE R+++ ++ + AAKY +E +L+ D Y R F+K+K
Sbjct: 266 HESTHESALRLVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTM 325
Query: 241 -----ARKGDESLENLGYIYFDESTG-----KFEVER--------SFLPSKPNAYGPVWL 282
+R G + LG I E K+ V + F + GP++
Sbjct: 326 TTYHCSRCGSYHNQPLGRISQREGRNNKTFTKYSVAQGPPVDTKCKFCEGTYHLAGPMYA 385
Query: 283 GPLKSQEIVEEMLEIS------QQLSLARKKQAVKLLKILKDEL-DVPLFYDTHGLGRRL 335
GPL ++E +EE+L I+ Q + +K+ +L + K+EL D P ++ + + +
Sbjct: 386 GPLHNKEFIEEVLRINKEEHRDQDDTYGTRKRIEGMLSLAKNELSDSPFYFSPNHIASVI 445
Query: 336 KIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
K++ +++++ L SLG+ S TH P+ +KT+AP++ V+
Sbjct: 446 KLQVPPLKKVVAGLGSLGFECSLTHAQPSSLKTNAPWDAIWYVM 489
>gb|AAD33093.1|AF081560_1 (AF081560) N2,N2-dimethylguanosine-specific tRNA methyltransferase
[Saccharomyces cerevisiae]
Length = 570
Score = 150 bits (375), Expect = 3e-35
Identities = 129/464 (27%), Positives = 207/464 (43%), Gaps = 113/464 (24%)
Query: 10 VLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLL---------------------- 47
V EGKA+IL PK E+ VFYNP NRD++V +
Sbjct: 45 VKEGKAEILFPKKET-----VFYNPIQRFNRDLSVTCIKAWDNLYGEECGQKRNNKKSKK 99
Query: 48 ----------------------------NVLKPRI-VLDALSATGIRGIRFALETP-AEE 77
N +P I +L+ALSATG+R IR+A E P E
Sbjct: 100 KRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVRE 159
Query: 78 IWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEK---HR 134
+ ND+ +A +K+N N E + N DDAN LM +
Sbjct: 160 VIANDLLPEAVESIKRNAEYN--------------SVENIVKPNLDDANVLMYRNKASNN 205
Query: 135 YFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCG-AHPKACMRKY-LAVPLR 192
FH IDLDP+G+ F+D A++S++ G++ +T TD + L G +P+ C Y A +
Sbjct: 206 KFHVIDLDPYGTVTPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVS 265
Query: 193 GELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG------------ 240
E HE R+++ ++ + AAKY +E +L+ D Y R F+K+K
Sbjct: 266 HESTHESALRLVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTM 325
Query: 241 -----ARKGDESLENLGYIYFDESTG-----KFEVER--------SFLPSKPNAYGPVWL 282
+R G + LG I E K+ V + F + GP++
Sbjct: 326 TTYHCSRCGSYHNQPLGRISQREGRNNKTFTKYSVAQGPPVDTKCKFCEGTYHLAGPMYA 385
Query: 283 GPLKSQEIVEEMLEIS------QQLSLARKKQAVKLLKILKDEL-DVPLFYDTHGLGRRL 335
GPL ++E +EEML I+ Q + +K+ +L + K+EL D P ++ + + +
Sbjct: 386 GPLHNKEFIEEMLRINKEEHRDQDDTYGTRKRIEGMLSLAKNELSDSPFYFSPNHIASVI 445
Query: 336 KIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
K++ +++++ L SLG+ TH P+ +KT+AP++ V+
Sbjct: 446 KLQVPPLKKVVAGLGSLGFECLLTHAQPSSLKTNAPWDAIWYVM 489
>gb|AAD29859.1| (AF086826) N2,N2-dimethylguonasine tRNA methyltransferase
[Saccharomyces cerevisiae]
Length = 554
Score = 150 bits (375), Expect = 3e-35
Identities = 129/464 (27%), Positives = 208/464 (44%), Gaps = 113/464 (24%)
Query: 10 VLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLL---------------------- 47
V EGKA+IL PK E+ VFYNP NRD++V +
Sbjct: 29 VKEGKAEILFPKKET-----VFYNPIQQFNRDLSVTCIKAWDNLYGEECGQKRNNKKSKK 83
Query: 48 ----------------------------NVLKPRI-VLDALSATGIRGIRFALETP-AEE 77
N +P I +L+ALSATG+R IR+A E P E
Sbjct: 84 KRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVRE 143
Query: 78 IWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEK---HR 134
+ ND+ +A +K+NV N E + N DDAN LM +
Sbjct: 144 VIANDLLPEAVESIKRNVEYN--------------SVENIVKPNLDDANVLMYRNKASNN 189
Query: 135 YFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCG-AHPKACMRKY-LAVPLR 192
FH IDLDP+G+ F+D A++S++ G++ +T TD + L G +P+ C Y A +
Sbjct: 190 KFHVIDLDPYGTVTPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVS 249
Query: 193 GELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG------------ 240
E HE R+++ ++ + AAKY +E +L+ D Y R F+K+K
Sbjct: 250 HESTHESALRLVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTM 309
Query: 241 -----ARKGDESLENLGYIYFDESTG-----KFEVER--------SFLPSKPNAYGPVWL 282
+R G + LG I E K+ V + F + GP++
Sbjct: 310 TTYHCSRCGSYHNQPLGRISQREGRNNKTFTKYSVAQGPPVDTKCKFCEGTYHLAGPMYA 369
Query: 283 GPLKSQEIVEEMLEIS------QQLSLARKKQAVKLLKILKDEL-DVPLFYDTHGLGRRL 335
GPL ++E +EE+L I+ Q + +K+ +L + K+EL D P ++ + + +
Sbjct: 370 GPLHNKEFIEEVLRINKEEHRDQDDTYGTRKRIEGMLSLAKNELSDSPFYFSPNHIASVI 429
Query: 336 KIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
K++ +++++ L SLG+ TH P+ +KT+AP++ V+
Sbjct: 430 KLQVPPLKKVVAGLGSLGFECLLTHAQPSSLKTNAPWDAIWYVM 473
>gb|AAF02109.1|AC009755_2 (AC009755) putative N2,N2-dimethylguanosine tRNA methyltransferase
[Arabidopsis thaliana]
Length = 562
Score = 149 bits (373), Expect = 4e-35
Identities = 108/355 (30%), Positives = 169/355 (47%), Gaps = 41/355 (11%)
Query: 54 IVLDALSATGIRGIRFALETPA-EEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVL 112
++ ALSA+G+R +R+A E ++ D + ++N+ F G + A+
Sbjct: 96 LIFQALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNI--KFNGSV------AIS 147
Query: 113 KSEKTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGA 172
K E +H DA M + F +DLDP+GSP FLD+A++SV G+L TATD A
Sbjct: 148 KVES----HHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMA 203
Query: 173 PLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFR 232
LCG + + C KY + PLR + CHE+ RIL+ + +A +Y I +L+ D Y R
Sbjct: 204 VLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVR 263
Query: 233 AFIKLKDGARKGDESLENLGYIYFDESTGKFEVE---RS--------FLPS--------- 272
F+++ A + L Y+Y F ++ RS +LP+
Sbjct: 264 VFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDC 323
Query: 273 -----KPNAYGPVWLGPLKSQEIVEEMLEISQQLS---LARKKQAVKLLKILKDELDVPL 324
K N GP+W P+ E V +L + + A + + L + ++ LDVPL
Sbjct: 324 NHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPL 383
Query: 325 FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
F H L LK + + + + YR S TH +P G+KTDAP EV +++
Sbjct: 384 FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIM 438
>gb|AAG12600.1|AC068900_6 (AC068900) putative N2,N2-dimethylguanosine tRNA methyltransferase;
628-4023 [Arabidopsis thaliana]
Length = 599
Score = 149 bits (373), Expect = 4e-35
Identities = 108/355 (30%), Positives = 169/355 (47%), Gaps = 41/355 (11%)
Query: 54 IVLDALSATGIRGIRFALETPA-EEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVL 112
++ ALSA+G+R +R+A E ++ D + ++N+ F G + A+
Sbjct: 133 LIFQALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNI--KFNGSV------AIS 184
Query: 113 KSEKTLVVNHDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGA 172
K E +H DA M + F +DLDP+GSP FLD+A++SV G+L TATD A
Sbjct: 185 KVES----HHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMA 240
Query: 173 PLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFR 232
LCG + + C KY + PLR + CHE+ RIL+ + +A +Y I +L+ D Y R
Sbjct: 241 VLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVR 300
Query: 233 AFIKLKDGARKGDESLENLGYIYFDESTGKFEVE---RS--------FLPS--------- 272
F+++ A + L Y+Y F ++ RS +LP+
Sbjct: 301 VFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDC 360
Query: 273 -----KPNAYGPVWLGPLKSQEIVEEMLEISQQLS---LARKKQAVKLLKILKDELDVPL 324
K N GP+W P+ E V +L + + A + + L + ++ LDVPL
Sbjct: 361 NHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPL 420
Query: 325 FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
F H L LK + + + + YR S TH +P G+KTDAP EV +++
Sbjct: 421 FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIM 475
>gb|AAD33092.1|AF081559_1 (AF081559) N2,N2-dimethylguanosine-specific tRNA methyltransferase
[Saccharomyces cerevisiae]
Length = 570
Score = 148 bits (371), Expect = 7e-35
Identities = 129/464 (27%), Positives = 207/464 (43%), Gaps = 113/464 (24%)
Query: 10 VLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLL---------------------- 47
V EGKA+IL PK E+ VFYNP NRD++V +
Sbjct: 45 VKEGKAEILFPKKET-----VFYNPIQQFNRDLSVTCIKAWDNLYGEECGQKRNNKKSKK 99
Query: 48 ----------------------------NVLKPRI-VLDALSATGIRGIRFALETP-AEE 77
N +P I +L+ALSATG+R IR+A E P E
Sbjct: 100 KRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVRE 159
Query: 78 IWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEK---HR 134
+ ND+ +A +K+NV N E + N DDAN LM +
Sbjct: 160 VIANDLLPEAVESIKRNVEYN--------------SVENIVKPNLDDANVLMYRNKASNN 205
Query: 135 YFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCG-AHPKACMRKY-LAVPLR 192
FH IDLDP+G+ F+D A++S++ G++ +T TD + L G +P+ C Y A +
Sbjct: 206 KFHVIDLDPYGTVTPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVS 265
Query: 193 GELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDG------------ 240
E HE R+++ ++ + AAKY +E +L+ D Y R F+K+K
Sbjct: 266 HESTHESALRLVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTM 325
Query: 241 -----ARKGDESLENLGYIYFDESTG-----KFEVER--------SFLPSKPNAYGPVWL 282
+R G + LG I E K+ V + F + GP++
Sbjct: 326 TTYHCSRCGSYHNQPLGRISQREGRNNKTFTKYSVAQGPPVDTKCKFCEGTYHLAGPMYA 385
Query: 283 GPLKSQEIVEEMLEIS------QQLSLARKKQAVKLLKILKDEL-DVPLFYDTHGLGRRL 335
GPL ++E +EE+L I+ Q + +K+ +L + K EL D P ++ + + +
Sbjct: 386 GPLHNKEFIEEVLRINKEEHRDQDDTYGTRKRIEGMLSLAKYELSDSPFYFSPNHIASVI 445
Query: 336 KIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
K++ +++++ L SLG+ TH P+ +KT+AP++ V+
Sbjct: 446 KLQVPPLKKVVAGLGSLGFECLLTHAQPSSLKTNAPWDAIWYVM 489
>gb|AAC33150.1| (AC005546) R29425_1 [Homo sapiens]
Length = 656
Score = 137 bits (342), Expect = 2e-31
Identities = 103/358 (28%), Positives = 163/358 (44%), Gaps = 47/358 (13%)
Query: 55 VLDALSATGIRGIRFALETPA-EEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLK 113
VL+ L+A+G+R IRFALE P + ND S A +L+++NV LN L + +
Sbjct: 156 VLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPS------ 209
Query: 114 SEKTLVVNHDDANRLMAEKHRY---FHFIDLDPFGSPMEFLDTALRSVKRKGILGITATD 170
DA LM + R F IDLDP+GSP FLD A+++V G+L +T TD
Sbjct: 210 --------QADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTD 261
Query: 171 GAPLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHY 230
A L G + C KY A+ L+ CHE+ RI++ + A Y + +L+ D Y
Sbjct: 262 MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFY 321
Query: 231 FRAFIKLKDGARKGDESLENLGYIYFDESTGKFEVER----SFLPS-------------- 272
R F+++ G K S ++ G F ++R S +PS
Sbjct: 322 VRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVT 381
Query: 273 --------KPNAYGPVWLGPLKSQEIVEEMLE-ISQQLSLARKKQAVK-LLKILKDEL-D 321
+ GP+W P+ + V +LE +S + ++ +L ++ +EL D
Sbjct: 382 PECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPD 441
Query: 322 VPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
VPL+Y L + + ++ + L +R S +H VKTDAP +++
Sbjct: 442 VPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIM 499
>gi|8923219 hypothetical protein FLJ20244 [Homo sapiens]
>gi|11425929|ref|XP_009198.1| hypothetical protein
FLJ20244 [Homo sapiens] >gi|7020206|dbj|BAA91031.1|
(AK000251) unnamed protein product [Homo sapiens]
>gi|11066198|gb|AAG28495.1| (AF196479)
N2,N2-dimethylguanosine tRNA methyltransferase [Homo
sapiens]
Length = 659
Score = 137 bits (342), Expect = 2e-31
Identities = 103/358 (28%), Positives = 163/358 (44%), Gaps = 47/358 (13%)
Query: 55 VLDALSATGIRGIRFALETPA-EEIWMNDISEDAYNLMKKNVLLNFKGELEESNGRAVLK 113
VL+ L+A+G+R IRFALE P + ND S A +L+++NV LN L + +
Sbjct: 156 VLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPS------ 209
Query: 114 SEKTLVVNHDDANRLMAEKHRY---FHFIDLDPFGSPMEFLDTALRSVKRKGILGITATD 170
DA LM + R F IDLDP+GSP FLD A+++V G+L +T TD
Sbjct: 210 --------QADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTD 261
Query: 171 GAPLCGAHPKACMRKYLAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHY 230
A L G + C KY A+ L+ CHE+ RI++ + A Y + +L+ D Y
Sbjct: 262 MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFY 321
Query: 231 FRAFIKLKDGARKGDESLENLGYIYFDESTGKFEVER----SFLPS-------------- 272
R F+++ G K S ++ G F ++R S +PS
Sbjct: 322 VRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVT 381
Query: 273 --------KPNAYGPVWLGPLKSQEIVEEMLE-ISQQLSLARKKQAVK-LLKILKDEL-D 321
+ GP+W P+ + V +LE +S + ++ +L ++ +EL D
Sbjct: 382 PECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPD 441
Query: 322 VPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFSPTGVKTDAPYEVFVNVL 379
VPL+Y L + + ++ + L +R S +H VKTDAP +++
Sbjct: 442 VPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIM 499
>pir||T47750 N2,N2-dimethylguanosine tRNA methyltransferases-like protein -
Arabidopsis thaliana >gi|7572931|emb|CAB87432.1|
(AL163763) N2, N2-dimethylguanosine tRNA
methyltransferases-like protein [Arabidopsis thaliana]
Length = 433
Score = 133 bits (332), Expect = 3e-30
Identities = 106/376 (28%), Positives = 170/376 (45%), Gaps = 41/376 (10%)
Query: 31 FYNPRMALNRDIAVVLLNVLKPRI----VLDALSATGIRGIRFALETPAEEIWMNDISED 86
F+ A RD+ V+ + K VLDA+ GIR +R+ +E A+ + ND ++D
Sbjct: 64 FFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMANDANDD 123
Query: 87 AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEKHR---YFHFIDLDP 143
++ N+ ++E G E+ VV H AN+ M E++ +F ID+D
Sbjct: 124 NRRVITDNL-----SKVERGTG-----DERRWVVTHMLANKAMIERYMVADFFDMIDIDS 173
Query: 144 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 203
FGS FL A +++ G+L +T+TDG G P + Y A +E+G R+
Sbjct: 174 FGSDSSFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRM 233
Query: 204 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 261
L+G R AA + + +YY H FR +++ G D N G++ G
Sbjct: 234 LIGGAVREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHED---RNYGFVTHCNLCG 290
Query: 262 KFEVER-------------SFLPSKPNAYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 305
R + S GP+WLGPL V EMLE++++ +S
Sbjct: 291 HSHTLRFDELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGT 350
Query: 306 KKQAVKLLKILKDELD---VPLFYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 362
KLL I+ +E D P + + R K+ + ++++++ L GY ASR+H
Sbjct: 351 GMDLDKLLSIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHII 410
Query: 363 PTGVKTDAPYEVFVNV 378
P +KTD P FV +
Sbjct: 411 PNALKTDCPMSHFVRI 426
>sp|P20300|YORZ_PYRWO HYPOTHETICAL PROTEIN IN GAPDH 5'REGION (ORF Z)
>gi|81091|pir||S10650 hypothetical protein Z -
Pyrococcus woesei (fragment)
Length = 73
Score = 120 bits (299), Expect = 2e-26
Identities = 58/73 (79%), Positives = 68/73 (92%)
Query: 3 ITLELVEVLEGKAKILTPKAESIYDAPVFYNPRMALNRDIAVVLLNVLKPRIVLDALSAT 62
+++EL EV EGKAK+L PKA++IYD+PVFYNPRMA NRD+ V+LLNVLKP+IVLDALSAT
Sbjct: 1 MSMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSAT 60
Query: 63 GIRGIRFALETPA 75
GIRGIRFALETPA
Sbjct: 61 GIRGIRFALETPA 73
>emb|CAC00605.2| (AL359776) N2,N2-dimethylguanosine tRNA methyltransferase
[Leishmania major] >gi|11071774|emb|CAC14618.1|
(AL449144) N2,N2-dimethylguanosine tRNA
methyltransferase [Leishmania major]
Length = 718
Score = 107 bits (264), Expect = 3e-22
Identities = 84/305 (27%), Positives = 126/305 (40%), Gaps = 76/305 (24%)
Query: 25 IYDAPVFYNPRMALNRDIAVVLLNVL------KPR---------IVLDALSATGIRGIRF 69
+ D VFYNP +NRD+++ ++ V +PR +L+ALSATG+R IR+
Sbjct: 62 LVDQAVFYNPAQVVNRDLSISVIEVFSRLRLTEPRRRGGTNEGITILEALSATGLRAIRY 121
Query: 70 ALE-TPAEEIWMNDISEDAYNLMKKNVLLN--------------------FKGELEESNG 108
E T I ND+ DA + + +N N + GE E G
Sbjct: 122 YKEITNVRYIIANDMDADAVDCIVRNCAYNGVPVQYPTLMANMPAAAIELYAGEAEAEKG 181
Query: 109 --------RAVLKSEKTLVVNHDDANRLMAE----------------------------- 131
R +++ ++ N DDAN LM
Sbjct: 182 ESATGASPRVGVRAGGAILPNLDDANDLMFRLAMNSNAHPGKRVCLVDAPAETSATASLR 241
Query: 132 ---KHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLA 188
+ +DLDP+GS FLD A R +K G++ +T+TD A LCG KY +
Sbjct: 242 PLLQQELMDVVDLDPYGSASPFLDGAFRCIKEGGLMLVTSTDSAILCGNFADTAHAKYSS 301
Query: 189 VPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDESL 248
+P + CHE R L+ V R A ++ I +L+ + D Y R F ++ + S
Sbjct: 302 MPYKAAHCHEAAVRTLLACVERVANRHQKFIVPLLSLHIDFYVRCFFRVYKQPAETKLSA 361
Query: 249 ENLGY 253
LGY
Sbjct: 362 CKLGY 366
>emb|CAC00613.1| (AL109865) bG120K12.3.1 (novel protein similar to archaeal, yeast
and worm N2,N2-dimethylguanosine tRNA methyltransferase
(isoform 1)) [Homo sapiens]
Length = 446
Score = 92.8 bits (227), Expect = 6e-18
Identities = 60/188 (31%), Positives = 101/188 (52%), Gaps = 10/188 (5%)
Query: 75 AEEIWMNDISEDAYNLMKKNVLLN-----FKGELEESNGRAVLKSEKTL---VVNHDDAN 126
A ++ +ND++E++ L+++N LN + +E + + + EK L V DAN
Sbjct: 13 AVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDAN 72
Query: 127 RLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKY 186
LM R F FI LDPFG+ + +LD+A R+++ GI+ +T+TD + L R Y
Sbjct: 73 VLM--HLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHY 130
Query: 187 LAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDE 246
+R E E+ RI+V VAR AA+ + GIEV+ A +H+ +++ G DE
Sbjct: 131 GCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADE 190
Query: 247 SLENLGYI 254
+ + + Y+
Sbjct: 191 TAKKIQYL 198
>emb|CAC00614.1| (AL109865) bG120K12.3.2 (novel protein similar to archaeal, yeast
and worm N2,N2-dimethylguanosine tRNA methyltransferase
(isoform 2)) [Homo sapiens]
Length = 301
Score = 92.8 bits (227), Expect = 6e-18
Identities = 60/188 (31%), Positives = 101/188 (52%), Gaps = 10/188 (5%)
Query: 75 AEEIWMNDISEDAYNLMKKNVLLN-----FKGELEESNGRAVLKSEKTL---VVNHDDAN 126
A ++ +ND++E++ L+++N LN + +E + + + EK L V DAN
Sbjct: 13 AVKVTINDLNENSVTLIQENCHLNKLKVVVDSKEKEKSDDILEEGEKNLGNIKVTKMDAN 72
Query: 127 RLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKY 186
LM R F FI LDPFG+ + +LD+A R+++ GI+ +T+TD + L R Y
Sbjct: 73 VLM--HLRSFDFIHLDPFGTSVNYLDSAFRNIRNLGIVSVTSTDISSLYAKAQHVARRHY 130
Query: 187 LAVPLRGELCHEVGTRILVGVVARYAAKYDLGIEVILAYYKDHYFRAFIKLKDGARKGDE 246
+R E E+ RI+V VAR AA+ + GIEV+ A +H+ +++ G DE
Sbjct: 131 GCNIVRTEYYKELAARIVVAAVARAAARCNKGIEVLFAVALEHFVLVVVRVLRGPTSADE 190
Query: 247 SLENLGYI 254
+ + + Y+
Sbjct: 191 TAKKIQYL 198
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.320 0.139 0.397
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 137589890
Number of Sequences: 2977
Number of extensions: 5641656
Number of successful extensions: 13621
Number of sequences better than 1.0e-10: 32
Number of HSP's better than 0.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 13478
Number of HSP's gapped (non-prelim): 59
length of query: 383
length of database: 189,106,746
effective HSP length: 57
effective length of query: 326
effective length of database: 154,992,987
effective search space: 50527713762
effective search space used: 50527713762
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 166 (69.1 bits)